LOCUS       DATMSK010000001         5939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_211231,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000001 DATMSK010000000
VERSION     DATMSK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211231"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VNZ84_00005"
     CDS             complement(<1..428)
                     /locus_tag="VNZ84_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HWU65359.1"
                     /translation="MQHLKAIFTTLCLLLPYTLHAADLSGLWQTTDDRTGKPRSLVRI
                     VENAGEYSAVVEKGLLATDTGEAVCDKCTDERKGQKIVGMTIAKHLKPSSKSHVYEDG
                     EILDPENGKTYKCKMTLSPNGNELEVRGFIGFSLLGRSQT"
     gene            complement(441..1988)
                     /gene="ubiB"
                     /locus_tag="VNZ84_00010"
     CDS             complement(441..1988)
                     /gene="ubiB"
                     /locus_tag="VNZ84_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HWU65360.1"
                     /translation="MHWLRLFHIIGVLVWYRLDIFFIRDEQPGIAHRLLNLLFFWRHA
                     PEKRAVRLRLALEKLGPIFVKFGQMLSTRRDLLPPDVADELTKLQDQVPPFAYAQVEA
                     IIQEAFSAPLATVYAEFNITPVASASVAQVHFATLHSGEQVAVKVLRPGIAAIIHRDI
                     ALLRTLAWWLKKLSSEGRRLKPEEMVDEFARHTEHELDLTLEAAHCAQLGRNFSDRRL
                     LVPTVYWDYSRKQVMTMQRMFGTPVRDTASLAAYGINLTQLAHEGVEVFFTQVFRDGF
                     FHADMHPGNIQVVTEGPDKGKFIALDFGIMGSLTPTDQYYLARNFLAFFNRDYRDVAV
                     AHIESGWVPADTNVEALETAVRAICEPIFEKPLKDISFGRTLLSLFQMSRRFGVNIQP
                     QLIMLQKTLLNIEGLGRELDPNIDLWHSAKPFLKRWMSEQIGWRSLLKSAKRELPYIL
                     THAAEMPRLFEQYLRNQTELSKQQSRFDALLTSQHRQATWQKWATTAIIVLVLLQFAS
                     LFLLFSH"
     gene            complement(1991..2584)
                     /locus_tag="VNZ84_00015"
     CDS             complement(1991..2584)
                     /locus_tag="VNZ84_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWU65361.1"
                     /translation="MFKPLIQFVLQHLMQQNSWTAPLLQPYAHKNILLDFKVAQVMLT
                     ILSHGDLAIAADSASADATIHLPPSLAMRLLRQDPLAHSLIKIEGDATLGMEVGKILS
                     AIRWDVEDDLSKVVGDIAAYQVVQLGQEKLQRWQTQAKNLGEMLVEYWQEERPIIAKK
                     THIEQFNRSVDALREDTDRLQQRVEKLLAADTPHESQ"
     gene            complement(2588..3355)
                     /gene="ubiE"
                     /locus_tag="VNZ84_00020"
     CDS             complement(2588..3355)
                     /gene="ubiE"
                     /locus_tag="VNZ84_00020"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HWU65362.1"
                     /translation="MQQNTDPQKTTHFGFETVAETDKQKKVGEVFHSVAQKYDIMNDV
                     MSAGMHRLWKRFAVDVSGVGPGDKVLDIAGGSGDLSRLFAKKVNTQSAVNPGTVILTD
                     INASMLGVGRDRMIDAGLSIPATQCNAEVLPFADETFNCVIVAFGLRNMTHKDVALRE
                     MQRVLKPGGRLLVLEFSKVWQPLEKLYDTYSFKLLPKFGKWIANDEPSYRYLAESIRM
                     HPDQETLKQMMHEASFGKVDYYNLTAGVVALHKGIKL"
     gene            complement(3339..3734)
                     /locus_tag="VNZ84_00025"
     CDS             complement(3339..3734)
                     /locus_tag="VNZ84_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWU65363.1"
                     /translation="MSGLHPDCPTPTLIQLHQGSHLLEIHFDNHTECMLSCEFLRVYS
                     PSAEVRGHGAGQEVLQLDKENVNITGIEPVGRYAIKLIFDDGHDTGLYSWDYLYYLAQ
                     HYESLWQDYVTKLSMAGHTRKEPKHAAKH"
     gene            3968..5923
                     /locus_tag="VNZ84_00030"
     CDS             3968..5923
                     /locus_tag="VNZ84_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HWU65364.1"
                     /translation="MENNRLAQQFDLYTRWRQSIADVLGEYRRWLADKQLSDVQIEER
                     IQQQLNRLREDKLNVAFVAEFSRGKSELINAIFFSGYGHRLLPSGAGRTTMCPTELRY
                     DTGKPVSLSLLPIETSTHQISISEYRRTPQAWSVVEFDAHSRESMVEAFKEVSRTRRV
                     TVEEAQSLGLYNPDQPDDAMLIGQDGLLEIPCWRYAVINFPHPLLKQGLVILDTPGLN
                     AIGAEPELTMSMLPNAHAVLFILGADTGVTKSEMEVWRRYISGARWKQKGRMAVLNKI
                     DGLWDPLKTDDEVETELYRQLQNTAELLGLPRDQILPTSAQKGLLAKVKGDQQLLHKS
                     RLLQLERNLSDELIPAKQEIVRESIQGEIEDMMQQTRITLETRLQSVLDQAEELRCLQ
                     GKNEDVIAQMMRKVKQDKVNFEVGLQRYQALRSVFSVQSNQVFHHLGLPALKSKVRET
                     RDTMMKAAFTKTMRQAMDDFFSDLKRRMMDADVHVHEIKKMMEAMYEKFSKEHGLLQK
                     TPPPFSTSRYLKALNKLETIYRDQFNTTFNMIAHEKLTLTSKFFETLASHVILEYEAA
                     NRDTESWLKAVIAPMESQMREHHVQLKRRVESIKRIYQATDTLEERIADLEQVELSIR
                     QQLAELEQLNGKAMFALAHEEVIVQAA"
BASE COUNT         1492 a         1415 c         1534 g         1498 t
ORIGIN      
        1 caggtctgtg agcggcccag caatgaaaaa ccgataaaac cacggacttc cagctcatta
       61 ccattcgggc tcaaggtcat tttgcatttg taggtcttgc cgttttcagg gtcaaggatt
      121 tcaccatcct cgtaaacatg actcttgctt gaaggtttca agtgtttggc gattgtcatg
      181 ccaacaattt tctgaccttt gcgttcgtca gtgcacttgt cacagactgc ttcacccgtg
      241 tcggtagcga gcaggccttt ttcaacaacg gcactgtatt caccagcatt ttcaacaata
      301 cggaccagcg atctcggttt gccggtgcga tcatccgtgg tttgccaaag gccactgagg
      361 tccgcggcat gcagggtata ggggagtaat aaacaaaggg tggtgaatat tgctttgagg
      421 tgttgcatgg ttaatctcct ttagtggcta aacagcagga ataaacttgc aaattgcagt
      481 aataccagca cgatgatggc ggttgtggcc cacttttgcc aagtggcttg ccggtgctgt
      541 gaggtgagca gggcatcgaa gcggctttgt tgcttgctga gctcggtttg gttgcgcaag
      601 tattgctcga acaggcgtgg catttcagcg gcatgggtca ggatataagg caattcccgt
      661 ttggctgatt tgagcaggct acgccagccg atctgttcac tcatccagcg tttgaggaaa
      721 ggcttggcgc tatgccataa atcaatattc gggtcgagtt cgcggcctaa cccttcgata
      781 ttgagcaagg ttttttgcag cataatcagt tgcggctgga tgttgacgcc aaatcggcgt
      841 gacatctgga acaggctcag caaggtgcgg ccaaaagaaa tatccttgag cggtttttca
      901 aaaatgggct cgcaaatcgc acgtaccgca gtttccaggg cttcaacatt ggtatccgcc
      961 ggtacccagc cagattcaat atgggcgaca gcgacatcgc ggtaatcgcg attgaaaaat
     1021 gccagaaagt tgcgcgcaag atagtactgg tcagtcggtg tcaggctacc catgatgcca
     1081 aagtcgagcg caatgaactt gcctttatcc ggcccttccg tgaccacctg gatattgcca
     1141 gggtgcatat cggcatgaaa aaaaccatcg cggaacactt gtgtaaaaaa gacttccaca
     1201 ccttcgtgcg ccagttgggt caggttaatg ccataagccg ccagtgaggc ggtatcgcga
     1261 actggggtac caaacatccg ctgcatggtc atgacttgtt tgcgtgagta atcccagtaa
     1321 accgtcggca ccagcagtcg gcggtcactg aaattgcggc ctagttgagc gcagtgtgca
     1381 gcttccaacg ttaaatccag ttcatgttcg gtatggcgcg caaactcatc taccatttct
     1441 tccggcttca ggcggcggcc ttcactggat agttttttga gccaccaagc cagtgtgcgc
     1501 aacaaggcga tgtctcggtg aataatggca gcaatgcctg ggcgcaggac cttgaccgca
     1561 acctgttcgc cgctgtgtag cgtggcaaag tgcacttgcg cgacggaggc gctggcaacc
     1621 ggcgtgatgt tgaattcggc atataccgtt gcaagcggtg cagaaaaggc ttcctgaata
     1681 atggcctcaa cctgtgcata ggcaaaaggg ggtacctgat cctgcagttt ggttaactca
     1741 tccgccacat ctggcggcaa aagatctcgg cgtgtggata gcatctggcc gaatttgacg
     1801 aaaataggcc ccagtttttc cagtgccaac cgcaagcgta cagcgcgctt ttctggcgca
     1861 tgccgccaga aaaagagtag gttgagtagt cgatgagcga tgccaggctg ttcatcgcgg
     1921 ataaaaaaga tatcaaggcg gtaccagacc agtacgccga taatatgaaa caaacgtaac
     1981 caatgcattc ttactggctt tcgtgagggg tgtcggcagc aagcagtttc tcaacacgct
     2041 gttgcagcct gtcagtgtct tctcgcagcg catcgacaga acggttaaat tgttcgatat
     2101 gcgttttttt ggcaatgatg ggacgctctt cctgccaata ttccaccagc atctcgccca
     2161 ggtttttggc ttgtgtttgc cagcgttgca gtttttcctg tcctagttgt accacctggt
     2221 aagcagcaat atctccgacg actttactga ggtcgtcttc gacatcccag cggatggcgg
     2281 acaatatctt accgacttcc atgcccagcg tcgcatcgcc ttcaatttta atcaggctgt
     2341 gggccaatgg gtcttgcctg agcaggcgca tcgccaggct gggtggcaga tggatggtgg
     2401 catcagcgct ggctgaatca gctgcaattg caaggtctcc atgactcaga atagtcagca
     2461 tgacctgtgc gaccttgaag tccaagagga tgtttttatg cgcataaggc tggagtagcg
     2521 gcgccgtcca gctattttgc tgcatcaagt gctgcagcac gaattgaatc agtggtttaa
     2581 acatgggtta gagtttaatc cccttatgca gggcaaccac gcccgcagtc aggttgtaat
     2641 aatcaacttt gccaaagctc gcttcatgca tcatttgttt gagcgtttcc tggtctgggt
     2701 gcatgcgtat gctttctgcc aggtaacggt aacttggctc gtcattcgca atccatttgc
     2761 caaacttggg cagcaactta aatgaatagg tgtcgtacag tttctctagc ggttgccaga
     2821 ctttggagaa ctctagtacc agcaggcgac cgcctggttt gagcacacgt tgcatttcgc
     2881 gcagggcaac atctttatga gtcatattac gcaaaccaaa ggcgacgatc acgcaattga
     2941 atgtttcatc tgcaaatggc aggacttcag cattacattg tgtagcagga atgctcagcc
     3001 ctgcatctat catgcggtcg cggccaaccc cgagcatgga agcattaatg tccgtcagga
     3061 tcactgtgcc agggttcacg gcagattgcg tgtttacttt tttagcaaac aggcgggaca
     3121 aatcgccgct gccgccagca atgtccaaca ctttatcgcc aggacccacg ccgctgacat
     3181 ccaccgcaaa gcgtttccat aaacggtgca tgcctgccga catcacgtcg ttcatgatgt
     3241 cgtatttttg tgcgacagaa tgaaatactt caccaacttt tttctgtttg tccgtttcgg
     3301 caaccgtctc aaagccgaag tgagtggttt tttgcggatc agtgttttgc tgcatgtttg
     3361 ggttccttac gggtgtggcc agccatggac agttttgtca catagtcttg ccacaggctt
     3421 tcataatgtt gtgccagata atacaaataa tcccacgaat agaggccggt atcatgaccg
     3481 tcatcaaaga tcagtttgat tgcatagcgg ccgaccggtt caatgccagt gatgtttaca
     3541 ttttccttat ccagttgcag tacttcctgg cctgcaccat gtccacgcac ctcagcagaa
     3601 ggcgagtaga cgcgtaaaaa ttcgcaagaa agcatgcatt cggtatggtt gtcgaagtga
     3661 atttccagca ggtgcgatcc ttgatgcagt tgtatcaggg tcggggtagg gcaatcaggg
     3721 tgaagaccgc tcatagtatt gaagaaatgg tggattgagt cgataacttt agttatgcgc
     3781 atgatagcag atgcatgcat tcagcgcata aaccaacatt gttgtatctt atggttgata
     3841 attcatttta tttatgaatt aatagctata cctgcatgat ttatttgaac ttttattatt
     3901 tcatgataga ttactaaaaa aacagataat catgaatagg ttaccttatc actgttaagc
     3961 aggtcctatg gaaaataaca gactggcaca acaatttgat ttgtacacac gctggcgtca
     4021 atcgattgct gatgtcttgg gggagtatcg gcgctggttg gccgataagc agctcagcga
     4081 tgtgcaaata gaagagcgta tacagcagca attaaaccgc ttgcgtgaag ataagctgaa
     4141 tgtggcattt gtcgcggagt tttcgcgcgg caagtccgag cttatcaacg caatcttctt
     4201 ttccggttac gggcaccgtt tgctgccttc tggcgccggg cgtactacca tgtgccccac
     4261 cgagttgcgc tatgacactg gcaaaccagt gagtttatcg ttattgccaa tagaaaccag
     4321 cacgcaccag atttccatca gtgaataccg ccgtacccca caggcatggt ctgtggttga
     4381 gtttgatgca cattcacgcg aaagtatggt tgaggcattt aaagaggtca gccgcacacg
     4441 tcgtgtcact gtagaagagg cacaatcgtt aggcttatac aatcccgatc agccggatga
     4501 tgccatgctc attggccagg atggtctgct tgaaatccct tgctggcgct acgccgtgat
     4561 taacttcccg catccattgc tcaaacaggg cttggtgatt ctggataccc cagggttgaa
     4621 tgcgattggt gcagaaccag agttaaccat gagcatgtta ccgaacgcgc atgccgtatt
     4681 gtttatcttg ggcgcggata ccggtgtcac taagtctgaa atggaagtct ggcgccgcta
     4741 tatcagtggt gcgcgctgga agcaaaaagg ccgcatggct gtactcaaca agattgacgg
     4801 cttatgggat ccgctcaaaa cagatgatga agtggaaact gagctttacc gtcaattaca
     4861 aaacacagcc gaattgctag gcttgccccg cgaccagatt ttgccgacct ccgcccagaa
     4921 aggcttgctg gctaaggtca aaggcgatca acagttattg cacaagagtc gcttgctgca
     4981 actggaaaga aatctttccg atgaactgat tcctgccaaa caggagattg tgcgtgaaag
     5041 catacagggt gaaattgagg acatgatgca gcaaacgcgc atcacgcttg aaacccgttt
     5101 gcaaagcgtc cttgatcagg ccgaagagct gcgttgccta cagggcaaga atgaagatgt
     5161 gattgcgcag atgatgcgta aggtcaagca ggataaagtc aattttgaag ttggcctgca
     5221 gcgctaccag gcattgcgta gtgtgttttc tgtgcagtcc aatcaggtat tccaccatct
     5281 gggcttaccg gcactcaaga gcaaagtgcg cgaaacgcgt gacaccatga tgaaagccgc
     5341 ctttaccaaa accatgcggc aggcgatgga tgattttttc agtgacctca agcgccgcat
     5401 gatggatgcc gatgtccatg tgcatgaaat caagaaaatg atggaggcca tgtatgagaa
     5461 gttttccaaa gagcatgggc tgttgcaaaa gacgccgccg ccattttcaa cctcacgcta
     5521 tttaaaagca ctcaataaac tggaaacaat ttaccgcgac cagtttaata ctaccttcaa
     5581 tatgattgcc cacgagaaac tgacgctcac cagcaagttt tttgaaaccc tggccagtca
     5641 tgtgatcctg gaatatgaag cggctaaccg cgatactgaa agttggctaa aagccgtgat
     5701 tgcgccgatg gaaagccaga tgcgtgaaca tcatgtacag ctcaaacgcc gtgtcgaaag
     5761 catcaaacgt atttaccagg caaccgacac gttggaagag cgtatcgctg atctggaaca
     5821 ggtggaatta tcaatccgcc agcaattggc agaactggaa cagctcaatg gcaaagccat
     5881 gtttgcgtta gcgcatgagg aagtgattgt gcaggctgct taaggacgct cctataaat
//
LOCUS       DATMSK010000002         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_398974,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000002 DATMSK010000000
VERSION     DATMSK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398974"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VNZ84_00035"
     CDS             <1..420
                     /locus_tag="VNZ84_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWU65365.1"
                     /translation="TPPVFAIYSVLLVTRWLRDEIGGLHNVAKRNALKAACLYQVLDD
                     HADFYHGRAAVADRSTMNVVFNLPNAEIEQAFLQAAQQAGFSGLNGHRSIGGIRASIY
                     NGLEVAAAEQLAEFMQTFRKCYKPSTPRATGTVAAYA"
     gene            complement(442..819)
                     /locus_tag="VNZ84_00040"
     CDS             complement(442..819)
                     /locus_tag="VNZ84_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65366.1"
                     /translation="MKLIKVSAIVLAVFTVILTIVFFTWWAFKDEAVFGSEKFDQTRW
                     MELSSSVQKDCRRGDMAYDLKNNILIRGAKKEAVMALLGRPSLEDANALEYDLGKCMH
                     VYHALRIYFDPDERLTHSQISSH"
     gene            complement(840..1301)
                     /gene="nrdR"
                     /locus_tag="VNZ84_00045"
     CDS             complement(840..1301)
                     /gene="nrdR"
                     /locus_tag="VNZ84_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWU65367.1"
                     /translation="MKCPFCGADDTQVVDSRLNEEGNSIRRRRRCQVCDKRFTTYETA
                     ELHLPQVVKQNGTREEFKREKLRVSFTRALHKRPVPTEYVDKAMDRIIQKVLGLGVRE
                     VPARLLGETVMQELKQMDQVAYIRFASVYRSFSDVDDFHNAIRDLDVTDSD"
     gene            complement(1323..2570)
                     /gene="glyA"
                     /locus_tag="VNZ84_00050"
     CDS             complement(1323..2570)
                     /gene="glyA"
                     /locus_tag="VNZ84_00050"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWU65368.1"
                     /translation="MFSFSRNLSVADPALAQMISSEVERQHQHIELIASENYTSPAVM
                     EAQGSQLTNKYAEGYPGKRFYGGCEFVDQVEQLAIDRLKALYGAEYANVQPHSGSQAN
                     QAVYFSILKPGDTVMGMNLGHGGHLTHGSPANLSGKLFNIVPYGLNDKEEIDYDEMER
                     IAVECKPKLLIGGASAYALRFDWARMADIAKKVGAYFMVDMAHYSGLIAASVYPNPVP
                     HADFVTSTTHKTLRGPRGGIIMAKAEFEKSINSNVFPSLQGGPLMHVIAGKATAFLEA
                     AQPEFKVYQEQVLKNASAMAKTLAARGLRIISGRTESHVFLVDLRPKGLTGKAADAFL
                     GQAHITVNKNAIPNDPESPFVTSGIRIGSPAITTRGFKEAEAEQVANLIADVLDNPES
                     AEVITAVKAKVHALTDKFPVYGK"
     gene            complement(2611..>2795)
                     /locus_tag="VNZ84_00055"
     CDS             complement(2611..>2795)
                     /locus_tag="VNZ84_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65369.1"
                     /translation="VVLWAAASSASAEFYAPVWCRLSRGGAPVSCVRKKGAGAGKNKE
                     AVFTCMLKVHAGVASW"
BASE COUNT          675 a          749 c          683 g          688 t
ORIGIN      
        1 accccaccgg tgtttgctat ctacagcgta ttactggtta cccgctggct acgggatgaa
       61 atcggtggtt tgcacaatgt ggctaaacgc aatgcgctga aagcggcctg tttataccag
      121 gtgttggatg accatgctga tttctaccat ggccgtgccg cggttgctga ccgctcaaca
      181 atgaacgtgg tatttaattt gccgaatgca gaaatcgaac aagcgttttt gcaggccgct
      241 cagcaggctg gattttcggg actgaatgga caccgtagca tcggtggcat ccgtgcctcg
      301 atttacaatg gactggaagt ggctgccgct gaacagctgg cagagtttat gcaaacgttc
      361 cgcaaatgtt ataagccctc tacaccacgc gctaccggaa ccgtcgccgc atacgcttaa
      421 agcgccttac acttataacc cttagtgcga agaaatctgg ctatgggtca agcgctcatc
      481 cggatcaaaa tagatacgta atgcatggta aacgtgcatg cacttgccga ggtcatattc
      541 aagcgcattg gcatcctcca ggctagggcg tccgagcagt gccatcacgg cttctttttt
      601 ggcgccacgg atcagaatgt tatttttcag gtcgtaagcc atatctccac ggcggcaatc
      661 tttttgtaca ctgctggaaa gctccatcca gcgtgtctgg tcaaactttt cgctgccaaa
      721 caccgcttca tctttaaatg cccaccaggt aaaaaataca atggtcagga tgaccgtgaa
      781 taccgccagt acaatcgcag acactttgat cagtttcatg cacgctgcct ttcagctggt
      841 taatcgctat cagtcacatc cagatcacgg atggcattat ggaaatcatc cacatccgag
      901 aaacttcggt agaccgaagc aaaacggata taagccacct gatccatttg tttaagctct
      961 tgcatcacgg tttcacccag cagccgggct ggcacttcac gcacccctaa accgagcact
     1021 ttctggatga tacggtccat ggccttgtcg acatattcag taggcacagg acgtttgtgt
     1081 agtgcgcggg taaatgatac gcgcagcttt tcacgcttga actcttcccg cgtaccgttt
     1141 tgcttgacca cctgcggcag gtgtaactct gcggtttcat aggtggtaaa acgcttgtcg
     1201 cacacctggc agcggcgacg acggcggata gaattccctt cctcattgag gcgggaatcc
     1261 accacctgtg tatcatcagc accgcaaaac ggacatttca tggctgaaag tcgccggaga
     1321 aattatttgc cgtagaccgg gaatttatcc gtcaaggcat gtaccttggc tttcaccgca
     1381 gtaatcactt ccgcactttc cggattgtcc agtacatcag caatcaagtt agccacttgc
     1441 tcggcttcag cctctttgaa gccacgggtg gtaatcgccg gtgaaccgat acggataccg
     1501 gaagtcacga atggactctc agggtcgtta ggaatcgcat tcttgttcac ggtaatgtgc
     1561 gcctggccca ggaaggcgtc agccgcttta ccagtcaagc ctttaggacg caggtcaacc
     1621 aggaatacgt gtgactcagt gcggccggaa ataatgcgca agcctcttgc agccaatgtc
     1681 ttggccatgg cactggcgtt tttcaatacc tgttcctgat ataccttgaa ctcaggttgt
     1741 gccgcttcca ggaaagcagt ggctttaccg gcgatcacgt gcatcaacgg gccaccttgc
     1801 aggcttggga acacattgga gttaatggat ttctcaaatt ctgctttggc catgatgatg
     1861 ccgccacgtg gaccacgcaa tgttttgtga gtggtggaag tcacgaaatc agcatgtggc
     1921 accgggttag gatacacact cgcagcgatc aggccggaat aatgtgccat atcgaccatg
     1981 aagtaagcac cgaccttttt ggcaatgtct gccatacgtg cccagtcaaa acgcaaggcg
     2041 tacgcagagg ccccaccgat caacagtttt ggtttgcatt caaccgcgat acgttccatc
     2101 tcgtcataat caatctcttc tttgtcgttc aggccataag gcacgatatt gaacaactta
     2161 ccagacaggt tagccgggga accatgtgtc aagtgaccgc cgtgacccag gttcatcccc
     2221 atcacggtat cgcctggttt caggatagag aaatatactg cctggttagc ctgtgaaccg
     2281 gagtgtggtt gcacgttagc gtattctgcg ccgtacaacg ccttcaggcg gtcaatcgcc
     2341 aattgctcca cctggtcaac aaactcacag ccgccataga aacgcttgcc tggataacct
     2401 tccgcatact tgttggtcag ctgggagcct tgtgcttcca tgactgccgg gctggtgtag
     2461 ttttctgatg cgatcagctc gatatgctga tgctgacgct ctacttcgga agaaatcatt
     2521 tgggccagag ctgggtctgc aacgcttaag tttcttgaaa agctaaacat gacgatcaag
     2581 tttccgtatt cacaaaaata aagcgggatt ttaccatgag gcgacccccg catgcacctt
     2641 tagcatgcac gtgaatacag cttctttatt tttgccagcg cctgcccctt ttttgcgcac
     2701 gcaagaaaca ggtgcgccgc cccggctcaa cctgcaccaa accggtgcat aaaattcagc
     2761 agatgctgaa ctggcagcag cccacaacac aacaa
//
LOCUS       DATMSK010000003         5844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_93962,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000003 DATMSK010000000
VERSION     DATMSK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93962"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..242)
                     /locus_tag="VNZ84_00060"
     CDS             complement(69..242)
                     /locus_tag="VNZ84_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HWU65370.1"
                     /translation="MLYYALLFLVISFIAGLLGFRGIESGAATISKVFFFIFLLLAIG
                     IALAAVLGISLFA"
     gene            569..1060
                     /locus_tag="VNZ84_00065"
     CDS             569..1060
                     /locus_tag="VNZ84_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65371.1"
                     /translation="MRLTYHPQFTGYQLGLLILALFMPTAVLAASDSAKVISAPVQKS
                     MNKAETRSKVIALLNMDEVLPDCQQQVTMTKVKTVLYSESGLTPEKIGVDWQGGKFDI
                     ATNVGENPVLSLDEIHAANQFIKVGKRYLIHFQLCGDGSQHSLINMYAEQSPARPARK
                     IKT"
     gene            1373..1828
                     /locus_tag="VNZ84_00070"
     CDS             1373..1828
                     /locus_tag="VNZ84_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HWU65372.1"
                     /translation="MDRKVFFSDGGIIISDSRFIASGYIYNIRDITSVRLGVIEAPRW
                     LGIFGMGSGLVLMLMEDTLFVVGGCFIALGVMVGVFSKTRYAVILETATGEHRVFSDT
                     SKHYIERVICALDAAMVHRVTSSIAERVGGFDNANESSISLPMSAPLIE"
     gene            complement(1872..2261)
                     /locus_tag="VNZ84_00075"
     CDS             complement(1872..2261)
                     /locus_tag="VNZ84_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65373.1"
                     /translation="MHEKEFVAKDGLTYTLRFSQTGEEILILRDREQVGVIDLALRKE
                     AKGAESFMITVLDLEKCKRIGIGEASLKFHKEIFHRPLRANGDVREGRHSGENQVTKE
                     GIGFIGNMRKKGVIELIDFHGAFSEDE"
     gene            2537..2917
                     /locus_tag="VNZ84_00080"
     CDS             2537..2917
                     /locus_tag="VNZ84_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HWU65374.1"
                     /translation="MEEAIYFSHQGVSVSNSRFFINGKTISIHNVSTVERRVLKPQLG
                     FARICILGGIALLLGSGSLPLVGIFSMIYGGVLWYLATPRYLVVIHTPAGEYHAYMSE
                     NSVDAENVMTALNVAIALKGDTRQ"
     gene            3134..4954
                     /locus_tag="VNZ84_00085"
     CDS             3134..4954
                     /locus_tag="VNZ84_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWU65375.1"
                     /translation="MATASTQPLKEPVIDPLPGMGAIPHDEGVAFRVWAPNAQRVSVI
                     GSFNDWQAGLDECISEGNGYWYIDMANAKPGDEYQFHLINGEQQLQRIDPYAKIVTNS
                     AGNGVIPPLDFFAPPPVQFQMPPWNELVIYELHIGTFNAKGEHVGTFDTAIERLPYLK
                     ALGINAVEVMPIAEFAGDYSWGYNPAHPYAVESAYGGPEAFKRFVAAAHEIGIAVIVD
                     VVYNHFGPSDISLWQFDGWQENNKGGIYFYNDWRSATPWGDTRPDYGRGEVRKYIFDN
                     AMMWLDDYHADGLRYDMTLYIRSVEASAQNLIAEGWSLLQWINSEVETRYPGKITIAE
                     DLQNSAELTLKREDGGAHFGSQWDAGFVHPVRAALIEIDDAHRSMAAVRDALLNQYNQ
                     DAFQRVIYTESHDEVANGKQRLVTEIDPSEQPGRYAIKRSCLGAALVMTAPGIPMLFQ
                     GQEFLRDQWFRDDRPIDWQRAEDYSDITALYRDLIGLRLNQQGYSAGLSGQHVAVYHV
                     NDHDKLIAMHRWKEGGRGDDVVVVFNFAEGRKDNYLIGLPYGGEWKLRFNSDATLYNA
                     QLDETPSTDISARQESHDGYEFSATITIGAYACLIYTREQ"
     gene            5126..5737
                     /locus_tag="VNZ84_00090"
     CDS             5126..5737
                     /locus_tag="VNZ84_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65376.1"
                     /translation="MAVNSINAYSALQTQSTSSVQQLKQTVNALDSALKSGDLQSAKD
                     AYAKLSQLKTIDSNSPLFTIGQALKKDDIQSALQVVQTNAADKTTPLAWQQLAKLTAG
                     TNTTESTLSANNKISSLLNYLASGSGDATSTSTNYTPGIINQVISQMNSVSSTVSLGS
                     STSAETTELYSQLAQQIKNNPQLGAILVERLKAQSSNGVFSYL"
BASE COUNT         1547 a         1311 c         1454 g         1532 t
ORIGIN      
        1 ctcgcttaaa gcaagggtgc gtcgtgaaga tataaataca gagtgcgttc tatccgtact
       61 acttcaattt aagcaaacag gctgatgccc aatacggctg ccaaggcaat accaatcgct
      121 aacaacagga aaataaagaa aaatactttt gaaatcgtgg cagcgccaga ctcaatgcct
      181 ctaaatccca gcaacccagc gataaaagaa atgactagaa acaacagcgc gtagtaaagc
      241 atagattggc cccttcatta acattgaaca tggagccatc ttaaaaggat ctcgccaatt
      301 aacatatcag cgattcactt gaaaattggt cagaatgtac ctacgcaaag gggatgttaa
      361 acttggcagc cagccagtgc attgacggtg aggtggaaaa atatcttacg tgcaagggtt
      421 aatccgcgta tattaacaag actgatcgcg ccatagctgt acatcatttg caggcggtta
      481 agtgagtgga tactcactat tgctagtcgc taaatacacg aaggcatttc actttcacct
      541 tattcaagcc tcaggaaagg agggttgtat gcgtttgact tatcatccgc aattcaccgg
      601 ctatcaatta gggttgctta tactggcatt gttcatgcca acggctgtat tggctgccag
      661 tgacagtgcc aaggtgatca gcgcgccggt acaaaaaagc atgaataagg cagagaccag
      721 gtcaaaagtc attgcgctgt tgaacatgga tgaagtgctg cctgattgcc agcaacaagt
      781 caccatgacc aaagtgaaaa cggtgcttta cagtgaaagc gggctgacac cggaaaaaat
      841 cggcgtagac tggcaagggg gcaagtttga tattgctacc aatgtcggtg aaaacccggt
      901 gttatccctg gacgagatcc atgcggcaaa ccagtttatt aaggttggca aacgttacct
      961 gatccatttt cagctatgcg gtgacggtag ccagcacagt ttgattaata tgtacgctga
     1021 acagtcacct gcacgcccag cacgtaaaat caaaacctga gttcgacgca tctaactaga
     1081 aagcatgcac cttctgcttt tacatatgca aataatgcac tgctctgccc tatacgcgcg
     1141 tatagtgaac ctgattgttt aatcgtcttg gtctcaactg cttcctttgg ttgcagtatg
     1201 aggcctttca atgtgctgtc tttgcttaaa aacctccctc gtgctttcca agtggtgttt
     1261 ttacttaagc aacaatagtt tcacaacgta acacgcatgc gtgtgggcat ctgggccgct
     1321 actgtttcat tccttcattg cctgctttta ataaagataa aaggagcatg ttatggatag
     1381 aaaagtattt ttcagcgatg gcggtatcat tattagtgac agccgattta tcgcgagcgg
     1441 ctatatatac aatatccggg atatcacttc tgtcaggctg ggggtgatcg aggcgccgcg
     1501 atggttggga atattcggca tgggctcagg tttggtactg atgctcatgg aagatacttt
     1561 atttgtcgtg gggggctgtt tcatcgcatt aggtgtgatg gttggagtat tttccaaaac
     1621 ccgatatgcc gtgatcctgg agactgcgac aggggagcat cgtgtttttt cagataccag
     1681 caagcattat atcgagaggg taatttgtgc tctggatgct gcaatggttc accgtgtgac
     1741 atcatcgata gcggagcggg ttggcgggtt tgacaatgcg aatgagtcaa gcatatcgtt
     1801 gccaatgtca gcgcctttga ttgaataatg ggtaggggat gaatgttatg gcgctgtcat
     1861 tcatcttcgc tttattcgtc ttcactgaag gcaccatgaa aatcaatcag ctcaatcacc
     1921 ccttttttac gcatgtttcc tataaagcct atcccttcct tggtgacctg gttttcgccg
     1981 gaatgcctgc cttctctcac atccccattg gcgcgtagtg gtcggtgaaa aatttcctta
     2041 tgaaatttca gggaggcttc ccctatgccg attcttttgc acttttccaa atccagcacg
     2101 gtaatcataa agctttcggc cccttttgct tccttgcgga gcgctaaatc aatgacgcca
     2161 acttgttccc tatcgcgcag gatcaagatt tcctcgccgg tctgactgaa gcgaagggtg
     2221 tatgttaggc catctttggc tacaaattct ttttcatgca tatgaaatcc ttttgcggtg
     2281 cccatgcata gtgggcgtca tgaaggcatg atagcgccta gtgtgcatcc atgtttgttt
     2341 ggtgacttaa ttttacaaaa tgggatgcca gtaacaatgc gctagctagg atttaatcgt
     2401 ttttccaaac cggtttccag gggggggcaa cgcaatgacc gtgggtaaat aggatggagt
     2461 gttgaattgt tatcaactgt taacaataaa ctatgttatg ttagcattac tgtattgatt
     2521 gcaatggaga tatgacatgg aagaagccat atacttcagc catcaaggcg tgagtgttag
     2581 taatagccgt tttttcatta acggtaaaac aatttctatc cataacgtca gtacggttga
     2641 acgtagagtg ctcaagcccc agcttggctt tgctcggata tgtattctcg gggggatagc
     2701 attgctattg ggcagtggga gcttacctct ggtaggtatc ttttcaatga tatatggggg
     2761 cgtgctatgg tatctggcca cgcccagata cctagtggtg attcatacac cagcaggcga
     2821 atatcatgcc tacatgagtg agaatagtgt ggacgcggag aatgtgatga ccgcgctgaa
     2881 tgtggcgatt gcgctaaaag gggatacccg gcaatgagag tccagtgtct actaaaacgc
     2941 gcaaggggcg tcatgccaag tgccctgggt aggcccaaat gcttgtaatc cacgctttcg
     3001 gttctgtttg cttgacagaa aatactgaca gcataatcag actgaatcgc cttcttttgc
     3061 ggcagttgat tttattacat tcttggtaat tgcaccgttt ctatagagcc tatcaattcc
     3121 aggaggtccc tttatggcta ctgccagcac gcaacctttg aaagaacctg tcattgaccc
     3181 actgcctggt atgggcgcta tcccgcacga tgaaggggtc gctttccggg tctgggcgcc
     3241 aaatgcacaa cgggtttctg tgattgggtc atttaacgac tggcaagcag gcctggatga
     3301 gtgcatcagt gaaggcaacg gctattggta tattgacatg gctaacgcca agccggggga
     3361 tgaataccag tttcacctga tcaatggcga acaacaatta caacgcatcg acccttacgc
     3421 caaaatcgtc accaattctg caggcaacgg tgtgattcca ccgcttgatt tttttgcacc
     3481 acccccagtt caatttcaga tgccgccctg gaatgaactg gtcatttatg agctgcacat
     3541 cggtacattt aatgccaaag gcgagcatgt agggacattt gataccgcta ttgagcggtt
     3601 gccatacctg aaggcactgg gcattaatgc cgttgaagtc atgccgattg ccgagtttgc
     3661 tggcgattat tcctggggct ataacccggc gcatccctat gccgtggaaa gtgcttatgg
     3721 cgggcctgag gctttcaaac ggtttgtcgc cgcagcacat gagatcggca tcgctgtcat
     3781 tgtcgatgtc gtgtataacc acttcggtcc ctcggatatt agcctgtggc aatttgatgg
     3841 ctggcaggaa aacaataagg gcggcattta tttttacaac gattggcgct ctgccacgcc
     3901 ttggggcgat acacgtccgg attatgggcg tggggaagtg cgcaaataca tttttgacaa
     3961 tgccatgatg tggctggatg attaccacgc cgacggactg cgttatgaca tgacactgta
     4021 tatccgtagt gtagaagcca gcgcacaaaa cctgattgct gaaggctgga gtttgctgca
     4081 atggattaac agtgaagttg agacgcgcta tccaggcaaa atcaccattg ctgaagacct
     4141 gcaaaatagc gctgaactga cactcaaacg cgaggatggt ggcgcccatt ttggcagtca
     4201 gtgggatgcg ggatttgtac atccggtgcg ggcggcattg atcgaaatag atgatgccca
     4261 tcgctccatg gctgcagtac gcgatgccct actcaatcaa tataatcagg atgcatttca
     4321 gcgcgtgatt tatacggagt cgcatgatga ggttgcgaat ggtaaacagc gcctggtgac
     4381 tgaaatcgac ccgagtgaac agcccggccg ctatgcgatc aagcgttctt gcctgggtgc
     4441 tgcactagtc atgaccgcgc caggcatccc catgttgttc caaggacaag agtttctacg
     4501 tgaccagtgg ttccgcgatg accgtcctat tgactggcag cgtgcagagg attattcgga
     4561 tatcaccgcc ctgtatcgag acctgatcgg acttcgcttg aaccagcaag ggtattctgc
     4621 cgggctgagc ggacaacatg ttgctgtgta tcatgtcaat gaccacgata aactgattgc
     4681 catgcatcgc tggaaagaag gtggcagggg tgatgacgtg gtggttgtat tcaactttgc
     4741 cgaaggccgt aaagacaatt atctgatcgg cttgccgtat ggcggagagt ggaaattacg
     4801 tttcaacagt gatgccacgc tgtataacgc gcaactggat gaaacgccct caacagatat
     4861 ttctgcgcgg caagaaagcc atgatggcta cgagttttct gccaccatta cgataggcgc
     4921 gtatgcctgc ctgatctaca cacgcgaaca ataactggtg aagattgcaa tgtgccgtat
     4981 gccctgctga ggcaatcaag tagtgtgctt tagcatgctt ggtttggtaa ttggcgggta
     5041 ttcatgttgt tgattgccat aataaaagtg gcatcacgga tttatattct cctctatatc
     5101 ttaaatgtag atcaaagggt gtgacatggc ggtaaacagc attaatgcct acagtgcctt
     5161 gcaaacgcaa agcacttcca gcgtgcaaca gctcaaacaa actgtgaatg ctcttgattc
     5221 agcgttgaaa tcaggcgatt tgcaaagcgc taaagatgct tacgcaaaac taagccagct
     5281 gaaaacgata gacagcaata gtccactttt cacgattggg caggcgctca aaaaagacga
     5341 tattcaatcg gctttacagg tggtacaaac caatgcggct gataagacga ctccgttagc
     5401 atggcagcaa ttggccaaac tcacagccgg gaccaacacc acagagagta ctttgtcagc
     5461 gaataacaaa atttccagcc tgcttaatta tttagcctcc ggaagtggag atgctacatc
     5521 aacctctaca aactacacac ctgggatcat taaccaagtg atatctcaaa tgaatagcgt
     5581 cagtagcacg gtctcattgg gaagcagcac gtccgctgaa accacagagt tgtactccca
     5641 attggcgcaa caaatcaaaa ataatccgca gctgggtgcg atacttgtcg agcgcttgaa
     5701 agcacaatct tccaatggcg tattcagcta tttataaaga cctgtagcgc agagtatttg
     5761 tgaaggattg aagccatcaa aataaacgtt gaatagctgt cgtttccggc agccattcaa
     5821 tcttcaattg caagagatac aaag
//
LOCUS       DATMSK010000004         4305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_211319,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000004 DATMSK010000000
VERSION     DATMSK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211319"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VNZ84_00095"
     CDS             complement(<1..774)
                     /locus_tag="VNZ84_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWU65377.1"
                     /translation="MQTRHSEQKEPMTYLQLFNIKIDVLDMEGSVARIFTWLDADACD
                     CHYVVTPNVDHVVKLSENASFKAAYDQASLVVADGKPVVLASKLLGKPLPGTVPGSDL
                     VPALFSHAQTRHSKELRVYLLGAGPGVAERAAEQIHAQWPNVRVVGLYSPPMGFEKSP
                     EECKKICAQINQTQADLLVIGLGAPKQELWVAKYQSELQVKAALCVGATIDFLAGEKP
                     RAPMWMRRCGVEWLHRMASEPKRLVSRYLNDAVVFPRLFL"
     gene            complement(1018..2199)
                     /locus_tag="VNZ84_00100"
     CDS             complement(1018..2199)
                     /locus_tag="VNZ84_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWU65378.1"
                     /translation="MQYIDLLWFVVGLVLFVPTSYFCLQIVMAFFHAPVTAASTTATP
                     SLAILVPAHNESNGIGVTLKSLLAQVDLPASIIVIADNCTDDTADVARQYGVTVIERQ
                     DDLKRGKGFALDFGMQYLKSILQSPEIVMIVDADCWVGPHAIPTLAQTAREHQRPVQA
                     LYLMVSPDASALKTRLAEFAWVIKNQVRPLGLRVMQGPCQLMGTGMAFPWAFISNADL
                     ANGHLVEDMKLGLDAANDGHAPVFCHEALVKSAFAANEAGADSQRTRWEHGHISMIFN
                     TGLPLVWKGLRTRNAELVAMALDICIPPLALLVMCLSAYILISALLAVFGYAGSLVLG
                     GTMFVLLFLAVMAAWAKFGRHAIPLWMLVYAPLYALRKIPLYLKYLVKRQVEWVRSQR
                     D"
     gene            complement(2324..3538)
                     /locus_tag="VNZ84_00105"
     CDS             complement(2324..3538)
                     /locus_tag="VNZ84_00105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU65379.1"
                     /translation="MKVAYFINQHPLVSTSFIRREINAIERAGHPVFRTAIRGWASPV
                     VDQGDFRERDMTRYVMLLSWPARLWVVCKTFLKMPLAFIRTLMMSLKLGFKSEKFFAY
                     SLIYFSHACLLRQWYAEKEIDHVHAHFGTNSTEVLMYCKMLGGPDYSFTVHGPEEFDK
                     NKLIHLKEKVQHARFVIAISAFTKSQLFRWVDFNDWSKINEVHCGLEREFYEVSPVPV
                     PDTHRLVCVGRLCEQKGQLLLVEAVALLAARGKKVELVLAGDGEMRTEIESQIHKHQL
                     GGQITITGWITSAQVREYMQHSSAMILPSFAEGLPVVVMEAMALKRPVLTTYVAGIPE
                     LVVHGKTGWLFPAGDVQGLADVIEEFMATPVAELSAMGEQGYARVVARHAVDTEVQKL
                     LHLFQALPATQH"
     gene            complement(3535..>4305)
                     /locus_tag="VNZ84_00110"
     CDS             complement(3535..>4305)
                     /locus_tag="VNZ84_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWU65380.1"
                     /translation="FYSVLMILIGFYLWKSQQFTEKSLLKKLLPFIVLAMVGMLMLTQ
                     TRSAWVAFFAFFTFYALFMERKYIPVLLVLPVIGLAIPEIRDRLLDLTSGNQVVAYAK
                     LNSYAWRKYLWDSALHWMQVSKYLTGYGADAFLHYSPMFFPLSTDKPTAAHNVYVQTF
                     FDLGLFGTLAYIWLFFKTGQTILRNVIHDKLLKTIALISIASYLMYSYSDNLLGYLNF
                     NWYFWFFLGATIGWQYRIKPVESPETSSSSVTTASGSV"
BASE COUNT         1153 a         1164 c         1030 g          958 t
ORIGIN      
        1 caagaatagt cgtgggaata caacagcatc attcagatac cgggatacca gtcttttcgg
       61 ctcagaggcc atgcggtgca gccactccac cccgcatcga cgcatccaca tcggagcacg
      121 tggtttctca cctgccagaa aatcaatggt cgcgcctacg cagagcgcgg ctttaacctg
      181 caactccgac tgatatttag ccacccataa ttcctgcttg ggtgccccca accctattac
      241 cagaagatca gcttgcgtct ggttaatctg agcgcaaatc ttcttacatt cctcagggct
      301 tttctcaaag cccatgggcg ggctatacaa acccactaca cgcacattgg gccattgggc
      361 atgaatttgc tcggcagcgc gttctgctac gccaggaccg gcgcccagca aatagacgcg
      421 caactccttg ctatgccgtg tttgtgcgtg gctgaacaag gcaggaacca gatcactccc
      481 tggaacggtg ccaggcaagg gcttccccaa caatttagaa gccagcacca caggcttgcc
      541 atccgctact accagggaag cctgatcata agccgctttg aaagaagcgt tttcactcag
      601 cttgaccaca tgatccacat tcggcgttac cacgtagtgg caatcgcatg catcagcatc
      661 gagccaggtg aatatgcgag ccacactgcc ctccatatcc agcacgtcaa tcttgatatt
      721 aaaaagctgg agatatgtca tcggttcctt ttgttcacta tgacgagttt gcatgcggtg
      781 tcgatcagtt gtcggttatc agttaagcaa atagcaggcc tgcgttactc cgtcggcctg
      841 aaataaatta cagtttataa acagaggcct caaatccgcc atgggcgcca cattccagcc
      901 tttgtctcca aacagcgacg tttaaaaaag gctggagaaa atagtctttt caaacaagct
      961 acccatcgtt atttcgaccg tctccaatca gcgacaagat cagaaaatgg gcaattatca
     1021 atcgcgttgc gatcgcaccc actccacttg gcgcttgacc agatatttca ggtacaaggg
     1081 gatctttctg agcgcataca acggcgcata caccagcatc cacaagggaa tggcgtgtcg
     1141 tccaaactta gcccatgccg ccatcactgc caggaacaga agcacgaaca ttgtgccccc
     1201 aagcaccaat gagccagcat aaccgaatac tgccagcagc gcagatatca gaatgtatgc
     1261 gcttaaacac atcaccagca acgccagggg aggaatacaa atatccagtg ccattgccac
     1321 aagctcggca ttgcgggtac gcaagccctt ccatacgaga ggaagtcccg tattaaaaat
     1381 catgctgatg tgcccatgtt cccagcgcgt gcgctgggaa tccgccccgg cctcattcgc
     1441 ggcaaatgca cttttgacca atgcctcatg gcaaaaaaca ggggcatggc catcattggc
     1501 ggcgtcgagg cctagcttca tgtcttcaac cagatgaccg ttggcaagat cagcgttgga
     1561 aataaatgcc cagggaaatg ccatgcctgt gcccatcaac tgacatggcc cttgcatgac
     1621 acgcaatccc aatggacgta cctggttttt aatgacccag gcaaactcag ccaaccgtgt
     1681 cttgagcgcg ctggcgtcgg gcgacaccat aaggtaaagc gcctgtacag ggcgctgatg
     1741 ctccctggcg gtttgcgcca gtgtgggaat ggcatgtggc ccaacccagc aatccgcatc
     1801 aacaatcatc acaatctcgg gggactgcaa aatgcttttg aggtattgca tgccaaaatc
     1861 cagcgcgaag cctttgcctc gcttgagatc gtcctgacgc tcgatgacgg tcacaccgta
     1921 ttgccgggca acatccgccg tatcgtcagt acagttatcc gcaatcacaa tgatgcttgc
     1981 gggtaagtca acttgcgcca gcagggattt gagcgtgacg ccaatgccat tggattcatt
     2041 gtgtgcagga accaatatgg ccaacgaagg cgttgctgtc gtagacgctg cggttactgg
     2101 cgcatgaaaa aatgccatga caatttgcag gcagaaatag ctggtcggaa caaacaggac
     2161 taggccgaca acgaaccata agagatcaat gtattgcatg aatcagtgtc acctattaat
     2221 cacgttcagt ctaaggataa agatagtcgt tacctgccag tcacattgga atatctgagc
     2281 gactggagca tttagcatgc gccacccggc gatgcatgga cgactaatgc tgggttgcgg
     2341 gcaatgcctg aaacaagtgc agcaactttt gtacctcggt gtccaccgca tgacgggcaa
     2401 ccacacgagc atatccctgc tcccccatgg cggacagttc tgctaccggc gttgccatga
     2461 attcctcaat gacgtcggcc agcccttgca catcgcccgc gggaaacaac catcccgttt
     2521 tgccatggac aacgagttca gggatgcccg caacataggt ggtaagcaca ggccgtttga
     2581 gtgccatggc ctccatgacc accacaggga ggccttcagc gaagcttggc aatatcatcg
     2641 cactggaatg ttgcatgtat tcacggactt gtgcactggt aatccagccg gtaatcgtaa
     2701 tctgcccccc caactggtgc ttgtgtattt gagattcaat ttcagtgcgc atttcaccgt
     2761 cacccgccag caccagctcc acttttttac cccgggcagc cagcaaggcg accgcctcta
     2821 cgagcaacaa ttggcctttc tgctcacaca agcggcccac acaaaccaga cgatgcgtgt
     2881 cggggaccgg caccgggctt acctcataaa attctctttc cagaccgcaa tggacttcgt
     2941 tgatttttga ccagtcgttg aagtctaccc agcgaaatag ctggctcttg gtaaaggcac
     3001 taatggcgat gacaaacctg gcgtgttgca ctttttcctt aaggtgaatc agtttgtttt
     3061 tatcaaactc ttccgggcca tgcacggtaa aactgtagtc gggtccgccc agcatcttgc
     3121 agtacatcag cacctcggtt gaatttgtcc caaaatgcgc gtgcacatgg tcgatctctt
     3181 tctcggcgta ccactgtctt aacaggcagg catgtgaaaa atagatcagg ctgtaggcaa
     3241 aaaacttttc tgacttgaag cccaacttca gggacatcat aagggttctg ataaatgcca
     3301 atggcatttt caaaaaggtt ttgcatacca cccacaaacg ggcaggccag ctaagcagca
     3361 tcacatagcg tgtcatgtca cgctcgcgaa aatctccctg atcgactacc ggacttgccc
     3421 agccgcgaat ggcagttctg aaaaccggat ggccggcacg ttcaatagcg ttaatttcgc
     3481 gacgaataaa actcgtactc accaaagggt gttgattaat aaagtaggcg accttcacac
     3541 gcttcccgat gctgtcgtga cagatgatga actggtttct ggcgactcca ctggtttgat
     3601 tctgtactgc caaccaatgg tagcccccag gaagaaccag aaataccagt tgaagttcag
     3661 gtaacccaac aaattatctg agtaagagta catcagataa cttgcgatgg agatgagtgc
     3721 aatagttttc aataacttat catgaatcac gttccgtaat atcgtttgcc ccgtcttgaa
     3781 aaagagccag atataagcca gcgtgccaaa gaggcccaga tcaaaaaatg tttgcacata
     3841 aacattatgc gccgctgtgg gtttgtcggt agatagcggg aagaacatgg gtgagtagtg
     3901 caagaatgca tctgccccat agccagtgag gtatttggag acttgcatcc aatgcaatgc
     3961 cgagtcccac aagtatttgc gccaggcata ggaattcaac ttggcatagg cgaccacctg
     4021 gttaccagag gtaaggtcga gtaatcgatc tcggatttca ggaatcgcca ggccaataac
     4081 ggggagcacc agcaatacag ggatgtactt gcgctccatg aacaaagcgt aaaaggtaaa
     4141 gaaggcaaag aaggcgaccc atgcactccg ggtctgcgta agcatgagca tgcccaccat
     4201 ggccagcacg ataaagggca gcagtttttt cagcagggac ttttcagtga actgctggct
     4261 tttccacaaa taaaaaccaa tcaaaatcat caaaacgcta taaaa
//
LOCUS       DATMSK010000005        14212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_375899,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000005 DATMSK010000000
VERSION     DATMSK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 14212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14212
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375899"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /locus_tag="VNZ84_00115"
     CDS             complement(<1..91)
                     /locus_tag="VNZ84_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-binding protein"
                     /protein_id="HWU65381.1"
                     /translation="MAQVEKTVLVMHSSAAMFQLVDAVEDYPSF"
     gene            173..628
                     /gene="smpB"
                     /locus_tag="VNZ84_00120"
     CDS             173..628
                     /gene="smpB"
                     /locus_tag="VNZ84_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWU65382.1"
                     /translation="MTIAQNKKAFFDYFIEEKYEAGIVLEGWEVKAIRDNRINLKESY
                     VVIMRGEIYLIGCHVTPLGSASTHVRPDAIRTRKLLLHNEEIAKLIGKVERSGYTIVP
                     LDMHFTRGRIKVQIGLAKGKKQHDKRDVEREKDWKREQSQLLKNKARER"
     gene            complement(638..1477)
                     /locus_tag="VNZ84_00125"
     CDS             complement(638..1477)
                     /locus_tag="VNZ84_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWU65383.1"
                     /translation="MTTYQSNAPSLWQSQLPKPNATSHKYSRGYVLVQGGYPTTGAAR
                     LAAFAAARTGAGITAIAVPEAALPIYASQLLSIMAKPYSTIEELETLIKDKRIGALLI
                     GPGAGVTEATYHTTLTMLDTGKPVVIDADALTAFAAQATQLKDAIKAPCIMTPHDGEF
                     ERLFGVAPARQRNDRIQQTLTAARQTGAVILLKGAQTVIAAPDGRVIVNEDAPATLAT
                     AGAGDVLAGIITSLLSQGMPALEAAAAGVWIHSEAARMFGIGLIADDLPDFIPAALAQ
                     LLA"
     gene            1609..3243
                     /locus_tag="VNZ84_00130"
                     /pseudo
     CDS             1609..3243
                     /locus_tag="VNZ84_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882041.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
     gene            3268..4107
                     /gene="kdsA"
                     /locus_tag="VNZ84_00135"
     CDS             3268..4107
                     /gene="kdsA"
                     /locus_tag="VNZ84_00135"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HWU65384.1"
                     /translation="MKLCGFDVGIEHPLFLIAGPCVIESKQMAIDTAGQLKEIAARVG
                     VPFIYKSSYDKANRSSTKTFRGFGMEEGLKILDEVRAQIGVPILTDVHTEEQVPHVAA
                     VVDVLQTPAFLCRQTDFIVACAQSGKPVNIKKGQFLAPGDMKQVVNKAKEANGGIDNI
                     MVCERGASFGYNTLVSDMRGLAIMRETNCPVVFDATHSVQQPGGQGDKSGGQREFVPV
                     LARAAVASGIAGVFMETHPDPSKALSDGPNAWPLGKMEDLLHLLVNLDKLVKKAGFAE
                     QTL"
     gene            4147..5433
                     /gene="eno"
                     /locus_tag="VNZ84_00140"
     CDS             4147..5433
                     /gene="eno"
                     /locus_tag="VNZ84_00140"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWU65385.1"
                     /translation="MSAIVDIIAREIMDSRGNPTVEVDVLLESGVIGRAAVPSGASTG
                     AKEAVELRDGDKSRYLGKGVLNAIENVNTEITEAIIGLDAEEQSFIDKTLIELDGTEN
                     KDRLGANAILGVSMACARAAAEESGLPLYRYLGGSAFMQLPTPMMNIINGGAHADNSV
                     DIQEFMIVPAGLPTFREALRAGAEVFHALKKTLHAKGLATTVGDEGGFAPNLPSNESA
                     LQLIMESIEAAGYEPGKDIYLGLDCASTEFHKDGKYHLASEGAALTSAEFCDYLATLA
                     GKYPIITIEDGMDEFDWNGWDLLTQRLGKTTQLVGDDLFVTNPKILREGIQKGVANSV
                     LIKVNQIGTLTETFQTIEMAKRANYTAVVSHRSGETEDTTIADISVATNALQIKTGSL
                     CRSERIAKYNQLLRIEEELGDATSYAGLSAFYQLFK"
     gene            5465..5893
                     /gene="ftsB"
                     /locus_tag="VNZ84_00145"
     CDS             5465..5893
                     /gene="ftsB"
                     /locus_tag="VNZ84_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HWU65386.1"
                     /translation="MRKLTVVLVVLIALLQYPLWLGKGSWLRVWQYAQQIEAHEKRNE
                     YYRQRNETLRAEVRDLKQGQSAIEERARSELGLVKQDEVFFQVIQNKQPGLSNKPTSP
                     LDAKPAAAKQLEAKPVDAKPAMVKPPDPNHDVPEHFPEAQ"
     gene            complement(5933..6934)
                     /locus_tag="VNZ84_00150"
     CDS             complement(5933..6934)
                     /locus_tag="VNZ84_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HWU65387.1"
                     /translation="MSHPATAWQQFLISQGAQFNPEGQASFPAVTANAHVFDLSATGL
                     IAISGADSQTFLQGQVTNDIKLLAQSAQFNGYCTPKGRLLALFYSFILEDVIYLQCPR
                     DLIPDLVKRLRMYVLRSKVLVEDASDRLLTVGLASDKLSTSIEHLPATPHQLASTNLG
                     ILIRLADAGNQQRALLVIQSEGAQACWQSLVATFTPSSTSAWEALEIQAGIPQVYAAT
                     KEQFVPQMVNLDALNGINFKKGCYTGQEIVARTHYLGKVKRRTLLAQLSSEQAPQAGD
                     ILQDAQQQEAGQLVRVAPATDRGWWVLAECRLEAKEAGTISWQGQPLAFKELPYALA"
     gene            7068..7451
                     /locus_tag="VNZ84_00155"
     CDS             7068..7451
                     /locus_tag="VNZ84_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65388.1"
                     /translation="MLVMISLWWTLPFAWAAVGMGVYGIASGWQWTQLAATSWKESIQ
                     TLRVDVYGQMTVTDRAGQTYQASVLPDSVVHPWCLILHLQLLSIAEDSAVESSSHHQR
                     ILLLPDQADSKTFSALRVWLRWGQD"
     gene            complement(7544..8581)
                     /locus_tag="VNZ84_00160"
     CDS             complement(7544..8581)
                     /locus_tag="VNZ84_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWU65389.1"
                     /translation="MTKKWLTFCLKHRDTLLLGIAMLLLLGAALQPSVPIKRNIHTYL
                     LIADITQSMNAVDMTLDHAPASRLDYTKRMMHDIIAQLPCQTRISIGLFAGDAVAALY
                     TPLHVCKHFSAIHQTIDHLDWRMAWTGNSQLRQSVPMTAKLLRQMPEETKAVIFTDGD
                     EAPLLHAFNTRNLKDFQGSDDFYFVGIGTDKGTGIPKLTETNQMIGYWSNESFAVQPN
                     IAQVTASKSSGRDDSVAVSDYDRFISRRATEYLKHLSTEIGGHYLDGDDPNAVLQKIQ
                     QHSSPRYEIIAYPIDWLLAILAGLCIISAYVNPGSKRLIQQRLRKRFSSIREVRLFKP
                     RHDIAHDNHLP"
     gene            complement(8645..9676)
                     /locus_tag="VNZ84_00165"
     CDS             complement(8645..9676)
                     /locus_tag="VNZ84_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWU65390.1"
                     /translation="MKIWLRHFIKHRDTALLALALLFLLGAILRPSIPFKHNIYAYFL
                     IADISQSMNAEDMMLHGKKVSRMAYMQNMMHEVVASLPCGTKVSVGMFAGNSVAAMYA
                     PIEVCNNFAAIQDTIAHLDWRMAWSGNSRVRESLFVTAKVVRAMPEPAQVLYFTDGEE
                     APKLHAFNTKNLDEFQGGNDWLFVGIGTEEGVAIPKLSPDNQVIGYWSNESFAMQPGI
                     AQISESTLGVRDDNVAFGDHDRYVSKLASEYLESLTKEIGAKFVKGDSTYTVLKAMKE
                     QKPARRDIAPLPLDWLLATLAGLCILATYASHHPWRQIKQNLHLYRREIQSRFFVKSE
                     AVAHDNHFQ"
     gene            complement(9729..10268)
                     /locus_tag="VNZ84_00170"
     CDS             complement(9729..10268)
                     /locus_tag="VNZ84_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65391.1"
                     /translation="MALSVNKLVIGLIVVSAAAVVTIAYSWQRIQQVEAFNQAVTTAK
                     SPQTDQQSFEAKYAVAYWLGKNERYKESTLLFTKLIEQANASQRAAILHNVGNMFFLR
                     AIQMTGGNDNSTRDEIEYLLSQAATSYKSALKADNSNWGTRRNYDRVLGLLPEKPTPG
                     VGESDNPGLIMGNIPNGLP"
     gene            complement(10253..11239)
                     /locus_tag="VNZ84_00175"
     CDS             complement(10253..11239)
                     /locus_tag="VNZ84_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HWU65392.1"
                     /translation="MAFTHPWLLWALPLALLPLLLERSHSKHYSWVGLLPPDPLSKTI
                     GFLLKVLAVISIVFIILGLAGPHSLEQQVERTGIGAQIGLVLDRSASMDDPFAGDKDG
                     NVGETKSVAAARLMTKFVQSRQNDMMGLITFSNSAMYVLPLTENKEAILSAIQATAGN
                     SLFQTNIGGGLTGAVELFKDMPDSGSRAIILISDGAGRVSDMVQQKLRDWLQRYNIGL
                     YWIVLRQPGGITIFDPKYTESGYDGPMPTEVLLYQFFQTLRTPFNAYEASDPKSLEAA
                     IADINSKEKKPIQYLEKIPGHDYTNVCYGIAFVMIAALLAVKYLEIHTWRSA"
     gene            complement(11239..12171)
                     /locus_tag="VNZ84_00180"
     CDS             complement(11239..12171)
                     /locus_tag="VNZ84_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65393.1"
                     /translation="MSASFNSVADVVLPSVSSKFVSVKEINPNRDAGFVVGDKLQRTM
                     ILTVKQPYELIKESLPIVGYEHRYRGQVSGIELTAIDVEEAHKSQQTVYTIHLEYQVF
                     KSGRVAKPAILRGEIVKLRQQGSRDIKQFRMPSFNFRTSPLSVYGEVNLKNEMSPFVA
                     PLQLHADKEWLALKVAAGVLVVCLLGLVYILGASTWLPRMGGAFAKAYRKIGKLPDDN
                     QGLQQAISTLHEAMHQVAGHSVFGNGIDHFLQQKPGFAAARPEIERFFALSRQVFFDA
                     NTPLDLGVPTREWLKKFCRHMRDCERGLKPEVKA"
     gene            complement(12232..13089)
                     /locus_tag="VNZ84_00185"
     CDS             complement(12232..13089)
                     /locus_tag="VNZ84_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWU65394.1"
                     /translation="MQTIKEFSYHTGWRSRSRRPGRHKSNQRGMGMEFRGHTTLLSYP
                     DPRRIDIRQTIRDPMEQVYVRIFNQKSATPVFVMCDMSGSMQYGTPHTKLVTAAEITR
                     SVAQSACRNTDPVGFIGFDDALHDEWLCTLSSRPHSMFDLADRLEDHLPNEVGAGALL
                     DSARLLPRERSLIFMVSDFHMPLEQLEAALLLMLRHHIVPVVLWNSTEYKQLPDFGIT
                     SINDPETGERRTLFLRASYRQRILEAFEARRQAIEDIFMRFDMQPFFVEDRFDADLLT
                     EYFHQYVPA"
     gene            complement(13133..14149)
                     /locus_tag="VNZ84_00190"
     CDS             complement(13133..14149)
                     /locus_tag="VNZ84_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWU65395.1"
                     /translation="MQEQQLNDWRTHALTLESEVNKVVVGQETPVRLITTAVFARGHV
                     LLEGDVGVGKTTLLRAFTRGIGGGYQRIEGTIDLMPNDLVYHTYLGEDGKPHVSPGPL
                     LMSGQDLSIFFFNEINRARPQVHSLMLRVMAERTLTAFNKEHHFPHMLVFADRNQVER
                     EETFDIPSAARDRFMMEIPIVVPPAHDIMESLIFDTRFHDVDQLISNVPADILKFDQL
                     NSFAKVIQENIQASPALRKYALDLWLATKDPVAYGVKVSNVDMKRLVLSGASPRGMGM
                     LLRAARVNAWLNNRAAVVPEDIHAVFHETIAHRLVFSPVYEMRRTEIAREMLSGIVNA
                     IAAP"
BASE COUNT         3433 a         3410 c         3825 g         3544 t
ORIGIN      
        1 ggaagctcgg gtagtcttct acagcgtcca ccaactgaaa catggctgca ctggaatgca
       61 tgactaaaac ggttttttct acttgtgcca tggtgaatga atcgtaccgg aaggaaacag
      121 ggttttgagt taaaatgcta ttttaagtga tttaagcctg cataccagcg ttatgaccat
      181 cgcgcaaaat aaaaaagcgt tttttgacta ctttatcgaa gaaaaatacg aagccggtat
      241 tgtgctcgaa ggttgggaag tcaaagccat tcgcgataac cgcattaact tgaaagagtc
      301 ttatgtggtc atcatgcgtg gggagattta cctgattggc tgtcacgtga cgccattggg
      361 ctcggcttct acgcatgtcc gtccagatgc gattcgtacg cgcaaactgt tattgcataa
      421 cgaagagatt gcaaaattga ttggtaaggt ggagcgctct ggttatacca ttgtgccact
      481 agacatgcat ttcacccgtg gccgtatcaa ggtgcagatt ggccttgcca agggtaaaaa
      541 gcaacatgac aagcgcgatg ttgagcgcga aaaagactgg aagcgtgagc aaagccagtt
      601 actgaaaaac aaggccagag aacgatagag gctgctttta agccagtaat tgtgcaagtg
      661 cggccgggat aaagtccggg agatcatccg caattaatcc aataccaaac attctggccg
      721 cttcactatg tatccacacc ccggcggctg cggcttctaa tgctggcatg ccttgtgaca
      781 gcaggctggt aatgatgccc gctaatacat ctccagcacc cgctgtggct aatgtagccg
      841 gtgcgtcttc attcacgatc actcggccgt ctggtgcggc aatcaccgtt tgtgcgcctt
      901 tgagcaggat gacagccccg gtttgcctgg cggcagtgag tgtttgctga atgcgatcat
      961 tacgttgcct ggcaggagcg acgccaaaca ggcgttcaaa ttcgccatcg tgtggtgtca
     1021 tgatgcaggg cgccttaatg gcgtctttca attgtgtcgc ctgcgctgca aaggcggtga
     1081 gtgcatctgc atctatcact accggtttgc cagtatcgag catggtaagc gtggtgtggt
     1141 atgttgcctc tgttacccct gcgccggggc caatcagcaa ggcgcctatg cgtttatctt
     1201 tgatcagcgt ttctagctcc tcaatagtgg aatagggctt ggccataatg ctgagtagct
     1261 ggctggcgta aatagggagt gcggcttcag gcactgcgat ggcagtgatg ccggcacctg
     1321 tgcgtgctgc ggcaaatgca gccagccgtg cggccccggt agtagggtat ccaccttgta
     1381 ccaatacata gccgcggcta tatttgtggc tagtcgcatt cggtttggga agttgtgatt
     1441 gccaaagtga tggggcgttt gattggtatg tagtcatgcg ccaattgtat gcccgagagg
     1501 cgtgattggc gagcatccct ctgattttat cggttacgtt cattgtgggc ggaagggctt
     1561 tgcggtaaaa tgttctcccg tctgtaacaa tctcaaagta ccttatccat gaccaaatat
     1621 gtattcgtaa ccggcggtgt tgtttcttct cttggaaaag gtatcgcagc cgccagtctt
     1681 ggcgcaattc ttgaatcacg tggcatcaaa gtgaccatgt tgaaactgga tccttatatc
     1741 aatgtagatc caggcaccat gtcgccattt cagcatggtg aagtatttgt aaccgatgat
     1801 ggtgctgaaa ccgatcttga tcttgggcat tacgagcgct ttattagcag caaaatgtcc
     1861 aagcgcaata acttcacgac aggccagatt tatgaaaccg tgattaaaaa agagcgtcgt
     1921 ggcgaatatc tgggtaaaac cgtgcaagtc attccgcaca tcaccgatga aatcaaggcc
     1981 catgtcaaac gcggtgccga aggggcagat gtggcgattg tggaagtcgg tggtaccgta
     2041 ggtgacattg aataactgcc tttcctggaa gccatccgcc agatggggat tgaagagggc
     2101 aggaataatg cctgctttat ccatttgact ttattgccct ggattccaac agcaggcgag
     2161 ctgaaaacca agccaaccca gcactcagtc aaagagttgc gtgaaatcgg tatccagccg
     2221 gatatcctga tgtgccgtgc cgagcgtgaa atccctgaag atgaaaaacg caagattgcc
     2281 ttgtttacca atgtggctta tgaggctgtg attaccgcca tcgatagcga ctccatttac
     2341 aaaattcctg gcttgctgca tgcgcagatg ctggatgaaa ttgtgtgcca taagctcaat
     2401 atcctggcga aagcagctga tttgtctgcc tggcaaaaaa ttacttatgc acaggccaac
     2461 ccgaaagaaa tcgtcgatat tgcctttgtg ggtaaatatg tcgacctgac agagtcatat
     2521 aaatccctga ccgaagcatt gattcatgcc gggatccata ccgagtccaa agtcaagatt
     2581 cactatattg acagtgaaga cattgaaaaa actggcacag atggccttaa aggcatggat
     2641 gcgatcctga tccctggcgg ttttggcgta cgcggcaccg aaggtaagat tgctgcgatc
     2701 cagtatgcac gtgaaaacaa agtgccttac ctaggtatct gtttggggat gcagctggct
     2761 gtgattgaat atgcgcgtca tgtggctggt cttaaagatg ccaacagtac cgagtttaac
     2821 cctaaagccg agcacaagct gatcggcctg attgatgagt ggcaggatgc ttccggcaaa
     2881 attgagaagc gtgatgaaaa ctctgaccta ggcggtacca tgcgtttggg cgcgcaaagc
     2941 tgtcctatcg tgccaaacac gctggcagcc agcatttatg gggctgaggt gaatgagcgt
     3001 caccgtcatc gttatgaagt caataaccac tatgtcgacc agttgaaagc cgctggtctg
     3061 gtggtttctg cacgtacgcc acgggaagag ttgtgcgaga tgattgagct gccacaaacc
     3121 gtgcacccat ggttcgtggc ttgccagttc caccctgagt tcacgtccaa tccgcgtagt
     3181 ggtcacccat tgtttaaagc ttatgtacag gccgccctcg cacagaaagc ctccaaaaaa
     3241 tagcgcctaa gaaaataagg aatactcatg aagttatgtg gttttgacgt tggtatcgag
     3301 catccgctgt ttttgattgc gggcccatgc gtcatcgaat ccaagcaaat ggcgattgat
     3361 accgctggcc agttgaaaga aatcgctgcg cgtgtgggtg tgccgttcat ttacaaatct
     3421 tcctacgaca aagccaaccg tagttcgacc aagacgttcc gcggttttgg catggaagag
     3481 ggcctcaaaa ttctggatga ggtgcgcgcg caaattggcg tgcctatcct gaccgatgtg
     3541 catactgagg agcaggtgcc acacgtcgct gcggttgtcg atgtgctgca aaccccggcc
     3601 tttctgtgcc gccagactga ttttattgtc gcttgtgcgc aatccggtaa gccggtgaat
     3661 atcaaaaaag gccaattcct ggcaccaggc gacatgaaac aagtggtcaa taaggccaaa
     3721 gaggccaatg gtggcatcga caacattatg gtgtgcgagc gtggtgcgtc ttttggctac
     3781 aacaccttgg tatcagatat gcgtggcttg gccatcatgc gcgaaaccaa ctgcccggta
     3841 gtttttgatg ccacccactc ggtgcaacag ccaggcggcc agggcgacaa gagtggcggc
     3901 cagcgtgaat tcgtgccggt gctggcacgt gcggcggttg ccagtggtat tgccggcgtg
     3961 tttatggaaa cacacccaga cccgagcaaa gccttgtcag atggtccaaa cgcctggcct
     4021 ttaggcaaaa tggaagactt actgcatttg ctggttaatt tggacaagct ggtaaaaaaa
     4081 gccggttttg ccgaacaaac cctataacta catccctttt tatttaaagt tagataagga
     4141 taaatcatga gcgctattgt agatattatc gcccgcgaaa ttatggattc acgtggcaac
     4201 ccgacggtgg aagtcgatgt gttgctggaa agcggtgtca tcggccgtgc tgccgtccct
     4261 tcaggtgctt ctactggtgc caaagaagct gtagagctgc gtgatggcga caagagccgt
     4321 tacctgggca aaggcgttct gaacgcgatt gaaaacgtca atactgaaat cactgaagcg
     4381 attattggcc tggatgctga agagcaaagt tttatcgaca aaactttgat cgagctggat
     4441 ggtacagaaa acaaagaccg tctgggtgcg aatgcgattc tgggtgtgtc catggcttgt
     4501 gcacgcgcag cggctgaaga gagtggtttg cctttgtacc gctacctggg tggctcagca
     4561 ttcatgcaat tgccaacgcc tatgatgaat atcatcaacg gtggcgcgca cgcggacaac
     4621 tctgtagata tccaagagtt catgattgtg ccagctggtt tgccaacttt ccgtgaagcc
     4681 ttgcgtgcag gcgctgaagt attccacgca ttgaagaaaa ccttgcatgc caaaggcctg
     4741 gcaacgaccg tcggtgatga aggtgggttt gcacctaacc tgccatccaa tgaatctgca
     4801 ttgcaattga ttatggaatc gattgaggcg gcaggttacg agccaggtaa ggacatttac
     4861 ctgggcctgg attgcgccag taccgaattc cataaagatg gcaaatatca cctggcatca
     4921 gaaggtgctg ccctgacttc tgccgagttc tgtgattacc tggcaaccct ggcgggcaaa
     4981 tatcctatca tcaccatcga agacggcatg gacgagttcg actggaatgg ctgggatctg
     5041 ctgacacagc gcctgggcaa aaccacgcag ctggtaggtg atgacctatt cgtgactaac
     5101 ccgaaaattc tgcgtgaagg catccagaaa ggtgtggcta actctgtgct gatcaaggtc
     5161 aaccagatcg gtaccctgac tgagactttc cagaccattg agatggcgaa acgtgcgaac
     5221 tacaccgctg tggtttctca ccgttctggt gaaactgaag atacgacgat tgcggatatt
     5281 tctgtcgcaa ccaatgcact gcaaatcaaa actggttctc tgtgtcgttc cgagcgtatt
     5341 gccaagtaca accagttgct gcgtattgaa gaagagctcg gcgatgcaac cagctatgct
     5401 ggcttgtccg cgttctacca gttattcaag taactgtttt cagccagatc actgataggt
     5461 caacgtgcgt aaattgacgg tcgtattggt ggtcctgatt gccctgctgc aatatccgtt
     5521 gtggctgggc aaaggcagct ggttgcgtgt ctggcaatat gcgcagcaga tcgaggcgca
     5581 tgaaaaacgc aacgaatatt accggcaacg taacgaaacc ctgcgtgcgg aagtgcgtga
     5641 cttgaaacag gggcaatctg cgatagagga gcgggcccgt agcgagttgg gtctggtcaa
     5701 gcaggatgaa gtattctttc aggtgatcca gaataaacaa ccggggctgt caaataagcc
     5761 aacttctcca ctagacgcaa agccagctgc tgcaaaacag ttagaggcta agccggtgga
     5821 tgccaagcca gcaatggtaa agccacctga tcccaatcac gatgttccgg aacattttcc
     5881 agaggcacaa taaaaaaggc gctacaagcg ccttttttat tcatgcgcat ttttatgcta
     5941 aggcataagg cagttcttta aaggccaatg gttgcccttg ccaactaatc gtgccagctt
     6001 ctttggcttc caggcggcat tcagccaaca cccaccagcc acgatctgtt gcaggtgcaa
     6061 cgcgtaccaa ctgtccggcc tcctgctgtt gggcatcttg caatatatcg cctgcttgtg
     6121 gtgcttgttc gctagaaagt tgcgccagca gggtacggcg tttgactttg ccaaggtaat
     6181 gcgtacgcgc tacgatttcc tggcctgtgt agcagccttt cttgaaattg atgccgttga
     6241 gtgcatccag gttgaccatt tgtgggacga actgctcctt ggttgcggca tagacctgtg
     6301 gaatgcctgc ttgtatttcc agtgcttccc acgcagatgt gctacttgga gtgaatgttg
     6361 ccactaatga ctgccagcac gcttgagctc cttcactctg aataaccagc agggcgcgtt
     6421 gctgattgcc cgcatcagcc aggcgtatca ggatgccaag gttggttgag gccagttgat
     6481 gtggcgtcgc aggcaggtgt tcaattgagg ttgagagctt gtcgctggcg agcccaacag
     6541 taagcaggcg gtcgctggca tcttcaacca gaaccttgct gcgtaataca tacatgcgta
     6601 agcgcttaac caagtcgggg attaagtcac gcgggcattg caggtaaatc acatcttcca
     6661 gtatgaagct gtagaacaat gccaataagc gtcccttagg ggtgcaatag ccattaaatt
     6721 gcgccgattg tgcaagcagt ttaatatcgt tggtgacctg gccctgcaaa aaagtctggc
     6781 tatctgctcc gctgatggca atcaaaccgg tggcagagag atcgaacacg tgtgcatttg
     6841 cggtcacggc cggaaaagag gcttgtccct ctggattgaa ttgagcgcct tgcgagatta
     6901 aaaactgttg ccatgccgtt gctgggtggg acataaataa ggtttccttc caggcttttg
     6961 ctatctagcc ttgggtaaat acaatgaaaa aagctatttt atcaagtgaa attgatttgt
     7021 gcttgcggcc atcaactctt ttatggggct ggtgggggct gttttcaatg ctggtgatga
     7081 tcagcctatg gtggacatta ccgtttgcgt gggcggctgt cgggatggga gtctatggca
     7141 tcgcaagcgg ctggcagtgg acgcaactag cggcgacatc atggaaggaa agcatccaga
     7201 cactgcgtgt ggatgtttat gggcagatga ctgtcacgga ccgggcagga cagacctatc
     7261 aagccagtgt gctcccggat agcgtcgtgc atccgtggtg tctgattttg catctgcaac
     7321 tgctatccat agctgaggat tcggcggttg aatcttcgtc acatcatcaa cgtatcctgc
     7381 tgctaccaga ccaggcagat agcaaaacct tttcagcatt acgggtctgg ttacgctggg
     7441 ggcaggatta gtggcggcgg ttgatgccag cttactcatg agagcttata accatgtcgg
     7501 gcaatcgcgt tcaagttgct tggaaagttg gctgaaagca gggttaaggc aagtggttat
     7561 cgtgcgcaat atcatgcctg ggtttaaaca ggcgcacctc tcttatgctg gagaatcgct
     7621 tgcgtagtct ctgctggatc agccgtttgg agccagggtt tacatacgca ctgataatgc
     7681 ataatccagc caggatggcg agtagccaat cgatggggta ggcgatgatt tcatagcggg
     7741 gtgaagagtg ttgctggatt ttttgcaata ctgcattggg atcgtcgcca tcaagataat
     7801 gtccgccaat ttctgtagac aggtgtttta ggtactcggt cgcccgccgg gaaataaagc
     7861 ggtcatagtc agatacagca acactgtcat cccggccact gcttttgctg gcagtgactt
     7921 gggcaatatt gggttgcacg gcaaaactct cgttggacca gtaaccgatc atctgattag
     7981 tttcggtcag tttgggaatc cctgtgcctt tatctgtccc tatccctaca aagtaaaagt
     8041 catccgaacc ctgaaaatcc ttgaggttgc gggtgttgaa tgcgtgcagt aggggcgctt
     8101 catcgccatc cgtaaagatg actgctttgg tttcctcggg catttgcctg agcaacttgg
     8161 cggtcatggg cacactctgg cggagctgac tgttaccggt ccatgccatg cgccagtcca
     8221 ggtgatctat ggtctggtga atggcagaaa aatgcttgca aacgtgtagc ggcgtataga
     8281 gcgctgccac ggcatcgcca gcaaacaggc caatactgat gcgtgtttgg caaggcagct
     8341 gcgcgatgat gtcgtgcatc atgcgcttgg tgtaatcaag gcggctggca ggtgcatgat
     8401 ccagtgtcat gtctaccgca ttcatgcttt gggtaatatc tgcaatcaac aggtaggtat
     8461 ggatattacg cttgatcgga acagaaggct gcaaggcagc ccccagcaat agcagcattg
     8521 cgatgccaag caataatgtg tcgcgatgtt tcaggcaaaa ggttagccat tttttggtca
     8581 tggtcatgtc ttctgtttgc ctgcatcgct ggtctaggat atcagagggg cgaggcgtta
     8641 acgattattg aaagtggttg tcgtgggcga cggcctcgct ttttacaaaa aatctagact
     8701 gaatctcacg acggtataaa tgcagattct gcttgatttg ccgccatgga tgatggctcg
     8761 cataggtcgc gagaatacat aaaccggcca gggttgctag cagccaatcc aagggcaagg
     8821 gggcaatgtc acggcgggcc ggtttttgct ccttcatggc cttgagtact gtgtaggtac
     8881 tatcaccttt aacaaatttt gcgccaattt ccttggtcag gctttccaga tattcgctgg
     8941 ccagtttgga gacatagcgg tcatggtcac caaatgcgac gttgtcgtct cgcacgccca
     9001 aggtgctttc ggagatctgt gcgataccag gctgcatggc aaaactctca ttagaccagt
     9061 agccaatgac ctggttatcc ggactcagtt tcggaatcgc cacgccttct tctgtgccga
     9121 tgccgacaaa cagccaatca ttaccgcctt gaaactcatc caggttttta gtgttgaaag
     9181 cgtgcagttt gggggcttct tcgccgtcgg tgaaatagag cacctgcgcc ggttccggca
     9241 tggcacgtac cactttagca gtcacaaaca ggctttcacg aacgcggctg ttaccagacc
     9301 atgccatgcg ccagtcgagg tgggcaatgg tatcctggat cgccgcaaag ttattgcaca
     9361 cctcaatcgg ggcgtacatg gcggcgaccg aattgccggc aaacatgcct acgctgactt
     9421 tggtaccaca gggcaaggag gcgaccactt catgcatcat attttgcatg taggccatgc
     9481 gcgagacttt tttgccatgc agcatcatgt cctctgcatt catgctttgc gagatatcag
     9541 caatcaggaa gtacgcataa atattatgct tgaaaggaat gctgggccgt aaaatggctc
     9601 ccagcagaaa tagcagcgct agggccagta gagccgtgtc gcgatgcttg ataaagtgtc
     9661 ttagccagat tttcatgcca tctcagtcaa tatgttcagg tccaggtgca aggcagaaaa
     9721 gctgcctgtt atggcaatcc attgggaata ttacccatga ttaatccggg gttgtcagat
     9781 tcgcctacgc ctggtgttgg cttttcaggt aacaagccca gtacgcggtc atagttgcgg
     9841 cgcgtgcccc agttactgtt gtctgctttg agtgcggatt tataagaagt agccgcttgc
     9901 gacaacaggt attcgatttc atcgcgcgtg ctgttgtcgt tgccaccggt catctgtatt
     9961 gcacgcaaga aaaacatatt gccgacattg tgcaagatag ccgcgcgctg gctggcattc
    10021 gcttgttcta tgagtttggt aaacagtagc gtggactcct tgtaacgctc atttttaccc
    10081 agccaataag caaccgcata cttggcttca aaactttgct ggtccgtttg cgggcttttg
    10141 gccgtcgtca ccgcctggtt aaacgcttcg acctgttgga tgcgttgcca actgtaagcg
    10201 atggtgacca cagcggcggc agatactact atcaggccaa taacgagttt gtttacgctg
    10261 agcgccatgt atgaatctcc aaatatttaa ccgccagcag ggctgcaatc atgacgaatg
    10321 caatcccgta acacacattg gtgtaatcat ggccaggaat tttttccaga tattgaatcg
    10381 gttttttttc tttgctgtta atgtcagcaa tcgctgcttc cagagacttg ggatctgagg
    10441 cctcgtaagc attaaaaggg gtgcgtagtg tctggaaaaa ctggtaaagc aacacttcgg
    10501 tgggcatagg gccatcgtag ccgctctcgg tgtatttggg gtcaaaaatg gtaatgcccc
    10561 caggttggcg cagcacaatc cagtacaggc caatgttgta gcgttgcagc cagtcacgca
    10621 gtttttgttg caccatatca ctgacacggc cagcaccgtc agaaatcagg ataatcgcgc
    10681 gtgagccaga gtcaggcata tccttgaaca gctccactgc acccgttaat cccccaccaa
    10741 tattggtttg aaacagcgag ttcccggcgg tggcctgaat ggctgacaag atggcttctt
    10801 tattttctgt cagaggtaac acatacatcg ccgaattact gaaagtgatc aggcccatca
    10861 tgtcattctg gcggctttgc acaaacttgg tcatcaggcg ggctgctgct acggacttgg
    10921 tttcacctac attaccatct ttgtcgccgg caaacgggtc atccatactg gcactgcggt
    10981 ccagcaccag gccgatctgt gcaccgatac ctgtacgctc cacttgttgc tccagactat
    11041 gcggtccggc caggccaaga ataatgaaaa cgatgctgat aacggctaat accttgagta
    11101 agaaccctat ggttttagac agcggatctg gtggcagcag gcctacccag gaatagtgct
    11161 tgctgtggga gcgttccagc aataacggaa gcagtgccag aggcagtgcc cacaacagcc
    11221 atggatgtgt aaatgccatt atgcttttac ctctggcttg aggccacgct cgcagtcgcg
    11281 catatggcgg cagaattttt tcagccattc acgtgttggt acacccaggt ccagcggcgt
    11341 gttggcatca aaaaatacct gacgggataa ggcaaaaaag cgttcaattt ctggcctggc
    11401 agcggcaaag cccggttttt gctgcaagaa atgatcaatg ccgttgccga atacgctgtg
    11461 accggccact tgatgcatcg cttcatgcag cgtggatatc gcctgttgca ggccttggtt
    11521 gtcgtctggc aatttgccaa tcttgcggta agctttggca aaggcgccgc ccatacgggg
    11581 taaccaggta cttgcgccca ggatatagac cagacctagt aagcaaacta caagaacccc
    11641 cgccgccacc ttgagtgcca gccattcttt atcagcatgc agttgcaggg gagcaacaaa
    11701 tgggctcatc tcatttttta aattgacttc accatagact gaaagcggtg aggtcctgaa
    11761 gttgaacgaa ggcatgcgga attgtttgat gtctctactg ccttgttgcc gcagtttgac
    11821 aatttcgcct ctgaggatgg ctggtttagc cacgcggccg gatttaaata cctggtattc
    11881 caagtgaatc gtgtacaccg tttgctggct tttgtgcgct tcttctacat cgatcgcagt
    11941 cagttcgatg ccagacactt ggccacggta acggtgctcg taaccgacaa tgggcagcga
    12001 ttccttgatc agctcatagg gttgcttgac cgttaaaatc atggtgcgtt gcagtttgtc
    12061 tcccacgaca aagcctgcat cgcggttagg attaatctct ttgacactga caaatttact
    12121 gcttaccgat ggcaaaacca catcggcaac actgttgaac gaggcagaca agagactggc
    12181 tgctaatatt aaacacaata gaggatggcg cgctttcatg ggtcgctttc tttaggcagg
    12241 cacgtactga tggaagtatt cggtgagtaa gtccgcatcg aatcggtctt ccacaaaaaa
    12301 cggctgcata tcaaatctca taaaaatatc ttcaatggcc tgacggcggg cttcaaatgc
    12361 ctccaggatg cgttgacggt aagaggcgcg taaaaaaagc gtccgacgtt cgccggtttc
    12421 cgggtcattg atactcgtga tgccaaagtc tggcaactgc ttgtattcag tactgttcca
    12481 taacaccacg ggcacaatat gatggcgtag cataagtagc agcgcggctt ccagctgctc
    12541 aagtggcata tggaaatcag acaccatgaa aatcagggag cgttcacgtg gcagtaacct
    12601 tgcgctatca agtaaggcgc cagcaccgac ttcattaggt aagtggtctt caagccggtc
    12661 agccaggtca aacatgctgt gcgggcggga agacagtgta cataaccact cgtcatgtaa
    12721 cgcatcatca aagccgataa aaccgaccgg atctgtattg cgacaggctg actgtgccac
    12781 cgagcgcgtg atttcggcgg ccgtgaccag cttggtgtgc ggtgtaccgt actgcatgga
    12841 gccagacatg tcgcacatga caaacacagg cgtggcgctt ttctggttga aaatacgcac
    12901 atatacctgc tccatcggat cacgtatcgt ctggcggata tcaatccggc gtggatccgg
    12961 ataggacagc agcgtggtat gccctctaaa ctccatcccc atgccacgct ggttgctctt
    13021 gtggcgacca gggcggcgtg agcgggagcg ccagccggtg tggtagctga attccttaat
    13081 tgtctgcatc acttttcagc actcgttatt ctgcacatca gcaggctatc aatcaaggcg
    13141 cggcaatcgc gttgacgatg cctgacagca tttcacgtgc gatttccgta cgacgcattt
    13201 cgtagactgg gctgaatacc aggcggtggg cgattgtttc gtggaataca gcatgaatat
    13261 cttctggcac taccgcggcg cggttattga gccaggcatt gacacgggca gcacgtagca
    13321 acatacccat accacgtggg ctggcaccac tcagcaccag gcgtttcatg tctacattag
    13381 agactttgac gccataggcc accggatctt tggtggccag ccacaggtcg agcgcatatt
    13441 tgcgcagggc agggctggcc tggatatttt cctggatcac cttggcaaag ctgttgagct
    13501 ggtcaaactt tagaatgtcg gccggcacat ttgagatcag ttggtctaca tcgtggaagc
    13561 gcgtatcaaa aatcagtgac tccataatgt cgtgtgcagg gggaaccaca atcgggattt
    13621 ccatcatgaa acggtcacgc gcagcagatg ggatatcaaa ggtttcttca cgctccacct
    13681 ggttgcggtc tgcaaatacc agcatatgcg ggaagtgatg ctctttgtta aaggctgtca
    13741 gtgtacgttc cgccatcaca cgcagcatca atgagtgcac ttgtggtcgc gcacggttga
    13801 tttcgttaaa gaagaagatc gaaagatctt gaccggacat cagcaatggt ccggggctga
    13861 cgtgaggttt gccatcttca cctagatagg tgtggtaaac caaatcgtta ggcatcaggt
    13921 cgatggtgcc ttcaatccgc tggtaaccgc caccaatgcc acgggtgaat gcgcgcagta
    13981 aagtcgtttt accgacaccc acgtcacctt ccagcaacac gtggccgcgt gcaaatactg
    14041 cggtggtaat caggcgtacc ggagtttcct gaccgacgac gactttgttg acttctgact
    14101 cgagtgtgag cgcatgggta cgccaatcgt tgagttgttg ttcttgcatg cctgatttcc
    14161 tgatgatttg aatatattaa gtggtttcga ttgtaaccaa attgtaactt ca
//
LOCUS       DATMSK010000006         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_141354,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000006 DATMSK010000000
VERSION     DATMSK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141354"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VNZ84_00195"
     CDS             complement(<1..1120)
                     /locus_tag="VNZ84_00195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWU65396.1"
                     /translation="MVNKTRESTIKALEFDPHDLACEELRHIYRGKNSSKLIVFFSGF
                     VAASMLAWDIGWQYAGIWYAWLLIAHTWHWWLGRLDFLSANVSIELARINRHIMAAAM
                     AGLGWGTLSIALPLLSRSSKAAILIIMLIAVIVKLPRLVVFLPIFFAFAAGVYVPLLL
                     TTPFLDFETLHMVLVMLIVVGVTLWASAREIRKILIDVLLKQVSSEQASWEDRLTGIG
                     NRRSFDAHLATTWMQASRAKVPCSLMMVDVDFFKKFNDTYGHQAGDDCLRQVASALDG
                     CVRRAGDSVTRYGGEEFAVVLFHTSLNDARNFSDHMLAAIRKLNIPHEQSPHKIVTIS
                     IGIATMMPTAEDDMTTFIEQADKSLYRAKENGRNRAEWH"
     gene            complement(1357..1896)
                     /gene="ribA"
                     /locus_tag="VNZ84_00200"
                     /pseudo
     CDS             complement(1357..1896)
                     /gene="ribA"
                     /locus_tag="VNZ84_00200"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
     gene            complement(1854..2021)
                     /locus_tag="VNZ84_00205"
     CDS             complement(1854..2021)
                     /locus_tag="VNZ84_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65397.1"
                     /translation="MHSNNQRLKAAPPALQPPVTLTASASFPPNTVLTFYTHLKTIER
                     VLSMLRWCWAT"
     gene            complement(2008..3048)
                     /locus_tag="VNZ84_00210"
     CDS             complement(2008..3048)
                     /locus_tag="VNZ84_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWU65398.1"
                     /translation="MPNSRAMSTSIRATAQVFEDPKSIALLNRIKQIAPSDANVLIIG
                     ETGTGKELIARHVHALSYRKSRPFVAVNCGAFSETLVESELFGHEKGAFTGAVTAKAG
                     WFEEANGGTLFLDEIGDLPLSIQVKLLRVLQEREVVRLGARKPTPIDVRLIAATNVRL
                     EEAVLAGNFREDLFYRLRVAHLELPTLRNRPGDILPLADYFVAEYTRRLGYASIRLDD
                     SAKQKLLQHSWPGNIRELENVIHHSLLICRNNVVRNEDLHLSSISLHRPKTALQVLPH
                     ETEADSLEASLLQLFESGKDQLYDYVEDTLVSAAYQHCHHNQVQTAKLLGLSRNVLRA
                     KLIKMGAINALK"
BASE COUNT          766 a          755 c          776 g          814 t
ORIGIN      
        1 tgtgccattc agctcggtta cggccgtttt ccttggctcg gtacagcgat ttatccgctt
       61 gttcgatgaa tgttgtcatg tcatcctcgg ccgttggcat catcgtcgcg atgccgatag
      121 aaatagtgac aatcttatgt ggtgattgct catgcgggat gtttaatttg cggatggccg
      181 ctagcatatg atcactgaaa ttacgggcgt cattcagtga agtgtggaat agcacaacgg
      241 caaattcttc accaccgtag cgtgtgacag agtcacctgc gcgacgtacg catccatcaa
      301 gtgcggatgc cacttggcgc aggcaatcat ctccagcctg atgaccgtag gtatcattga
      361 attttttaaa aaaatcgaca tccaccatca tgagtgaaca aggtactttt gcgcgtgagg
      421 cttgcatcca ggttgttgcc aggtgggcat caaaacttcg gcggttgcca ataccggtta
      481 acctgtcttc ccaagaggcc tgttcagagg acacttgctt tagcagaaca tcaatcagga
      541 ttttgcgtat ttcgcgtgcc gaagcccaca aagtaacacc tacaacgatc agcatgacca
      601 acaccatatg cagggtttca aaatccagaa atggagttgt tagcaaaagg gggacgtaca
      661 cccctgccgc aaaggcaaag aaaataggta agaatacaac caggcgtggc aatttcacga
      721 tcacggcgat cagcatgata atcagaatgg ctgctttgct ggagcggcta agtagtggaa
      781 gtgcgataga cagggtaccc caacccaatc cagccatcgc tgctgccatg atatggcggt
      841 taatacgcgc caactcaata ctgacattgg ctgagaggaa gtctaggcgt cccaaccacc
      901 agtgccaagt atgggcaata agcagccatg cgtaccatat tccggcatat tgccaaccga
      961 tatcccacgc cagcatgctg gcagcgacga atccgctgaa aaatacaatt aacttgctac
     1021 tgtttttgcc gcggtaaata tgacgcagtt cctcacaggc cagatcatgc gggtcgaatt
     1081 ctaaagcctt tatggtactt tctcttgttt tgttgaccaa attcgatcct gttccaacat
     1141 gctattgcaa gccactcatt cgagacgatg agtgattagt tgtatttatt atgctgtatt
     1201 tgtaggaatg aatatagtca gcaggctttc attttttaaa ataaaaaaat aaaacgcgac
     1261 attagtacaa tttaattgac aatggcgtgc tgtggttctg tttatcagtt tgcgccaaag
     1321 acatgattca tgccgatctc aatgttactt gtatcgttat gaaaagatat tggtatcaga
     1381 aagcaagctg agctgatgcc ccagtttatg ctttttggca gctaggtagc ctgcgttatg
     1441 taggtgactt gcgacctcaa gtgacaacac cgcttccacg gaaatgccct tatccattag
     1501 tgcttttgat ttgtcaggat tattactaag caagcggata gagtaaatct gtaaatgatg
     1561 caagatgctc gcggcaatgc tgtagtctcg gccatctact ggtaatccca gcgcttgatt
     1621 ggcttctact gtatccaggc cttgctcttg cagtgcataa gcgctgattt tattgctcag
     1681 cccgatgccg cggccttcgt gcccgcgcag gtagatgatc actccttgcc cctgtcgttg
     1741 tatcagttgc tgcgcttgct tgaattgctc tccacaatcg cagcgttgtg agccaaaaat
     1801 gtccccagtc agacattcgg aatgcacgcg tgtgagtatg ctggtgtggc cagttacgtc
     1861 gcccagcacc agcgcaacat gctcaatact cgttctatgg tcttcaaatg tgtgtaaaag
     1921 gtaagtaccg tattgggtgg gaagctggcg ctggctgtta gggtgaccgg cggttgtaaa
     1981 gcgggaggag ctgcttttag gcgctgatta tttgagtgca ttgatcgctc ccattttaat
     2041 cagtttggcg cgcagcacat tgcggcttaa gcccagcagc ttggcagttt gtacctggtt
     2101 gtggtggcaa tgctgataag ctgcactcac cagggtatct tccacatagt catagagctg
     2161 gtctttgcct gattcaaaca actgcaacaa cgaagcttcc agtgaatcgg cctctgtttc
     2221 gtgtggcaaa acctgcaatg cggttttggg acggtgtagt gaaatcgagg acaaatgcaa
     2281 atcctcattg cgcactacgt tgttgcggca aatcagcaag gagtgatgaa tcacattttc
     2341 cagttcgcgt atattgcctg gccaactgtg ttgtaataat ttttgtttgg ccgagtcatc
     2401 gaggcggatg ctggcatacc caagccttcg cgtatattca gctacaaaat aatctgctag
     2461 gggcaagata tcaccgggcc ggttgcgtag ggtcggcagt tccagatggg caacgcgaag
     2521 gcggtagaat aaatcttccc tgaagttacc ggccagcacc gcttcttcga ggcgtacgtt
     2581 ggttgctgca atcagccgca catcaatcgg ggttggtttg cgcgcaccca gtcgcaccac
     2641 ttcccgctct tgtagcacac gtaacagttt cacctggatg ctcagcggta aatcgccaat
     2701 ctcgtctaaa aacagcgtgc cgccattggc ttcttcaaac cagcctgctt tggcagtgac
     2761 cgcaccggta aaagcgcctt tttcatggcc gaacaattca ctctcaacca gcgtttccga
     2821 gaaggcaccg caattcacgg cgacaaacgg cctggattta cggtaactca aggcatgcac
     2881 atggcgggca atcagttcct tacccgtgcc ggtctcgccg atgatcagga cattggcatc
     2941 actgggggca atttgcttga tgcgattgag caaggcaatc gacttcggat cttcaaacac
     3001 ctgggcggta gcccgaatcg aagtcgacat cgcccgcgag ttaggcatgg ttaacagccg
     3061 ctgttgctca aactgcgtat cgtgttcagt ctcgtaaaaa tcgtttgcta g
//
LOCUS       DATMSK010000007         9894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_235216,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000007 DATMSK010000000
VERSION     DATMSK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235216"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VNZ84_00215"
     CDS             complement(<1..972)
                     /locus_tag="VNZ84_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWU65399.1"
                     /translation="MLAFPFVTVQAEESVLPDQAQTMTEVKVKAQKTADIPSEKTKSY
                     TVPASTTATKMSQSLKDTPQSISVVTRTLMDDFHLNNINDVLDYATGIKVERVEPSRN
                     YYTARGSDITNFQIDGIGVPFAYGLVFGDIDTAVYDRIEVLRGANGLMTGAGNPSATI
                     NFIRKRPTVDTQAKISAGVGSWDYRRLDADVSGSLNEAGSVRGRLIAASQDGNSYLDR
                     YSTERNVLSGIIEMDLSEQTMLTVGHTFQQHNADGVMWGSLPLLYSDGSKRHYSRSAS
                     TSPDWTYWHTKNNVTFAELQHIFDNEWKLKVQATRRETNAISKLFYVY"
     gene            complement(1159..1962)
                     /locus_tag="VNZ84_00220"
     CDS             complement(1159..1962)
                     /locus_tag="VNZ84_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU65400.1"
                     /translation="MTTLPAVSELLALERVTVSLGRQSFGPFTLSIRPGEQVAILGPS
                     GAGKSTLLKLLARELMPTSGQAHFDQRPLSDWSLADLSRRRAILPQSHEVAFGLPVEL
                     VIGLGRVARTYDSNLNSIVQQAAGQACAMHLLGRRFDTLSGGEKARVHMARVFAQLWD
                     AEQGLLLVDEPLAALDPGLQFELMDAINTFASRRGHAILAILHDINQALQGFERLILI
                     KHGKLVGDYQTSHVSLSLLEALYGVCLVCATTDEGELIVAPSLKRNVPA"
     gene            complement(1965..3041)
                     /locus_tag="VNZ84_00225"
     CDS             complement(1965..3041)
                     /locus_tag="VNZ84_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWU65401.1"
                     /translation="MTSFISPSSRMARTICGLPQQFASQPAWLLSLLLLLGIWAATAM
                     GAVTVTWQDWLAVLQSSDQPLSGSAYVLWHLRLPRILFAVLIGATLALSGALAQGLFR
                     NPLADPGLLGVTSGAACAAALTIVLLASSGIDVPVALRYWLLPTTAFAGAVAVCFILD
                     TVARWLTADSIAGLLLAGIALNALAAAVIGLCTYLATDEQLRTLSFWTLGSLAGANWT
                     LVGALLATLMLAFWALKQLTHTMNALALGEAAAAHVGVDVRALRVRVIMLVALLSGFA
                     VAWCGMIGFIGLVAPHIVRLLVGPDQRRLAPLAMLVGAMLLLLADTAARTVAIPAEIP
                     VGIFTALLGAPFFLVLLARMRRGV"
     gene            complement(3085..4005)
                     /locus_tag="VNZ84_00230"
     CDS             complement(3085..4005)
                     /locus_tag="VNZ84_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter substrate-binding protein"
                     /protein_id="HWU65402.1"
                     /translation="MSYVFNKQDENTPSSRRTFLGRMGAAVTVVCLPQLAFGNEAGKK
                     TARRIVSVGGGLTEILYALGAEKELVGVDTTSLYPQAATKLPSVGYARALSSEGILAL
                     SPTLVIATEDAGPPAVLRQIVTAGVPVSVLSANDKFEGVIERVRHVGALIEQTTRASQ
                     LEAQLKQDWQRARAPILARKTSAPRVLFILSHSPAQIMVGGRKTSADAMIAYAGARNA
                     VDGFDGFKPLTPEAVIAAQPDIVLFTEQGMSVVGGMDGALKLPGLAQTPAGQKRRIVA
                     FEAMFMLGFGPRLPQALSALDQALSNAMQS"
     gene            complement(4076..4624)
                     /locus_tag="VNZ84_00235"
     CDS             complement(4076..4624)
                     /locus_tag="VNZ84_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWU65403.1"
                     /translation="MSDTEKLQAVGAEAAAFPTQFQSIQLATTNAQGIPEASYAAYVQ
                     HEHKFYVYVSELATHCANLRDTGRCSVMFIEGEKDAAHLFARKRLVFQCGAQEVSRED
                     ARFDLVMGLFFEKFGQFMDVISKLTDFRLIELTPAHGSYVSGFAKAYHLNGQDLTQVM
                     HRNDQGHTTPDKTTQQKMDQLA"
     gene            complement(4810..5016)
                     /locus_tag="VNZ84_00240"
     CDS             complement(4810..5016)
                     /locus_tag="VNZ84_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65404.1"
                     /translation="MVTHLFMPNKKLPQIFFWPMLIALVSLAGLIFALVYDDAREYLA
                     NAAIALPVLVALFFYWVQPLFSRR"
     gene            complement(5058..6197)
                     /locus_tag="VNZ84_00245"
     CDS             complement(5058..6197)
                     /locus_tag="VNZ84_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HWU65405.1"
                     /translation="MSKQSSIRTWAWVHKWSSLVCTVFMLLLCLTGLPLIFHHEIGDW
                     LGNHIETPEIAYQGPMASMDAMLAAAKARQPGMVPLYMYREEDEPMQWVISMAKTATA
                     DSGFKQVVLDARTAKVLGEPPVEEGFMYIMFKLHVDLFAGLPGMLFLGLMGFLLLVAI
                     VSGVVLYAPFMRKLAFGEIRRQRGKKLKRLDVHNFLGIVTLSWALVVAATGVINAWSD
                     LLVKYWQFDQMSQMIAPYKNLPPPDRFASLQASVKVAQQTEPDMQLGFIAFPGTAYAS
                     PHHYGIFMRGDSPLTKRLFKPVLIDARTATLTDSRELPWYLVALLISQPLHFGDYGGM
                     TLKWLWAVLDVITIVVLWTGLMLWWKKRHQYVPDIRSRITLSEAY"
     gene            complement(6224..6640)
                     /locus_tag="VNZ84_00250"
     CDS             complement(6224..6640)
                     /locus_tag="VNZ84_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWU65406.1"
                     /translation="MGQNPFSAAEHTQPMSEINTTPLVDVMLVLLVIFIITAPLLTHA
                     VKIDLPQATSQPLEEKPEVIDLAVDAEGKVFWNDSEIQVADLAARMSEEAGKQQQPEL
                     QIRADKNTRYQVLAEVLAKAQTAGMKKVGFVSQPQP"
     gene            complement(6640..7383)
                     /locus_tag="VNZ84_00255"
     CDS             complement(6640..7383)
                     /locus_tag="VNZ84_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWU65407.1"
                     /translation="MQTTSGLAFITEGGVVSVLVALILLTMSVLSWYFILVKSWQVFK
                     RKRDVSAYVRQFWRKATLQEALQEAQLQSPAFRLALTAVESTQHHQQYASQSLAAACS
                     QDEFIARQLRRTLSQEQAALESGLSVLASVGSVAPFVGLFGTVWGIYHALTAIGQSGV
                     ATIDKVAGPVGEALIMTAIGLAVAIPAVLAYNAFIKANRGIMAELESFAQDLHTLLTT
                     GAPLVIKAGSKPANVTELHAKVANKGGNV"
     gene            complement(7430..8293)
                     /locus_tag="VNZ84_00260"
     CDS             complement(7430..8293)
                     /locus_tag="VNZ84_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWU65408.1"
                     /translation="MLQRESLRGLSLKPERASAPRLPELSLHRRQLGELAGKFRWSVL
                     ATEDWLSRDNRQGWHGLGMLAVVALHGAVIAWLVMHEEPVIVEPKPEPMTVSLITLPA
                     KKEEAPAPEVVPIIKKQPVVKTVVKPKETPKPIERPAPVVERIVETTPEQPKFEASTQ
                     PSAQPLEAPAAAAPSKPAPPAAAPKQEVVEEKVEPPKFGVAYLNNPAPEYPRLSQRAG
                     EEGRVLMKVLVSTEGRPESVEVINSSGFERLDKAAVNAVKQWRFEPARKGGKALSAYV
                     NVPLSFSLTQN"
     gene            complement(8372..8599)
                     /locus_tag="VNZ84_00265"
     CDS             complement(8372..8599)
                     /locus_tag="VNZ84_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HWU65409.1"
                     /translation="MTNRLQSSLGEVQNATGVSAQRMPTIDAQQLLGEAGVLLIMHGG
                     EAYQLTRTKQNKLLLTKCQAFMAADLVQQKQ"
     gene            complement(8798..9415)
                     /gene="rpsD"
                     /locus_tag="VNZ84_00270"
     CDS             complement(8798..9415)
                     /gene="rpsD"
                     /locus_tag="VNZ84_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWU65410.1"
                     /translation="MARTTGPRLKIMRALGVELPGLSRKSIENRPNPPGQHGQQASRR
                     RRSDFAVKLVEKQKIRFNYGVSEKQMRRLMLEARKGSEPTGDKLMSLLERRLDNVVFR
                     AGFAPTGIAARQLVNHGHVLLNGKSVNIASVRLKVGDVVSLKEKSRKIPMVVESLATP
                     SLTVPEWLSVEAASVSAKIGHLPSIEDVPFPIDVQQVVEYYANRV"
     gene            complement(9467..9775)
                     /locus_tag="VNZ84_00275"
     CDS             complement(9467..9775)
                     /locus_tag="VNZ84_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65411.1"
                     /translation="MQIDYAAQASDFFDPDLPTASAVLVSLSCVATRYAANPSEDLAL
                     LGCSLAQTLTAPEYAESGLIQSAAQQLLQDWQALLHAHQEFAMQQAVTDFVTQPATLQ
                     "
BASE COUNT         2509 a         2766 c         2340 g         2279 t
ORIGIN      
        1 atacacataa aacaacttgg aaatcgcatt ggtttccctt ctagttgctt gcaccttcag
       61 tttccactca ttatcaaaga tgtgctgcaa ctcggcaaaa gtgacattat tctttgtatg
      121 ccagtaggtc caatccggtg aagtggatgc tgagcgtgaa tagtggcgct ttgaaccatc
      181 agaatatagc agtggcaagc tgccccacat gactccgtca gcgttgtgct gttgaaacgt
      241 gtgtccaacc gtcagcatcg tttgctctga caaatccatt tcaataattc cactaagcac
      301 attgcgttca gtggagtagc gatccagata ggagttgcca tcctggcttg cggcaattaa
      361 acggccacgc acagatcccg cttcatttaa actccctgaa acatccgcat ccagcctgcg
      421 ataatcccaa ctaccaacac ctgccgatat tttggcttgc gtatccactg ttggacgctt
      481 gcgaataaag ttaatggttg ctgaaggatt acccgctcca gtcattaaac cattagcacc
      541 acgcaaaact tcaatccggt cataaacagc ggtatcaata tcaccaaata ccagaccgta
      601 agcaaatgga accccgatgc catcaatctg aaagtttgta atatctgaac cacgtgccgt
      661 gtagtagttt ctgctcggtt caacccgttc aaccttgata ccggttgcgt aatccaatac
      721 atcgttgatg ttgttcaaat ggaagtcatc catcaaagtt cttgtcacta ctgaaataga
      781 ttgcggcgta tccttaagtg actggctcat tttagtcgca gtagttgagg cgggaacagt
      841 gtaggacttg gtcttttctg aaggaatatc tgcagtcttt tgcgccttta ctttcacttc
      901 cgtcatggtt tgcgcttggt caggcaggac ggattcttca gcttgcacag taacaaatgg
      961 gaaagccagc atcaacgcta gcgaagtcgt attaatacga aacgagttca ttcaaagctc
     1021 cagtaattaa tcgctggcta caggagaaat aactcgttgg cttgcactaa gctttaaatg
     1081 ataacaatta tcatttaaag cttaaagaga ggttatttaa aagtgttgta tcgaactgta
     1141 tccttttatt acttaacgct aagctggaac gttcctcttc aaactaggtg caacgatcaa
     1201 ttcgccttcg tcggttgttg cacagaccag gcagacgcca tacaacgctt caagcaatga
     1261 aagcgagaca tgtgatgttt gataatcacc aaccaacttg ccatgcttaa tcaatatcaa
     1321 gcgttcaaag ccttgcaagg cctggttaat atcatgcagg attgccaaaa tggcatgtcc
     1381 gcgacgggag gcaaaggtat taatcgcgtc catcaactcg aactgcaaac ctggatccag
     1441 cgccgctaag ggctcatcaa caagcagcag accttgttct gcatcccata actgggcaaa
     1501 taccctggcc atgtgtacgc gtgctttttc accaccagag agggtgtcaa aacgtcggcc
     1561 aagcaggtgc attgcacaag cctgcccggc agcttgctgc acaatactgt ttaaattgga
     1621 atcatacgtc cgggcaacgc gcccaaggcc gatcaccagc tcaacaggca ggccaaatgc
     1681 cacctcatgg gattgcggca aaatggcacg gcggcgactc aggtcagcga gggaccaatc
     1741 acttaaaggg cgttgatcaa agtgtgcttg cccggaagtg ggcatcaact cacgcgccag
     1801 cagtttgagt aaagtggatt taccagcgcc actcgggccc aaaatagcga cctgctcacc
     1861 tggccgaatg gatagcgtaa aggggccaaa cgactgcctg cccagcgaca ctgtcactcg
     1921 ctcaagcgca agcaattcag aaacggcagg caaagttgtc atgctcaaac gcctctgcgc
     1981 attctggcca gcaacaccag aaaaaacggc gcacccagga gtgctgtaaa aataccgacc
     2041 ggaatttcag caggaatcgc caccgttctg gcggcagtat ctgccagcag caacaacatg
     2101 gcgcccacca acatcgccaa aggagccaag cggcgctggt ctggcccaac cagcaggcgc
     2161 acgatgtgtg gcgctaccaa gccaataaac ccgatcatgc cgcaccaggc gactgcgaag
     2221 ccgcttaaca aggccaccag cataatcacg cgtacccgta atgcacgcac atcgacgcct
     2281 acatgtgcag ctgcagcttc gcccagtgct aaggcattca tggtatgggt gagttgtttg
     2341 agtgcccaga acgcaagcat gagtgtagcc agcaatgccc ccaccaatgt ccaattggcc
     2401 ccagctaggg agcctaacgt ccagaatgac aaggtccgta gctgctcgtc tgtggccaga
     2461 tatgtacata agccgatcac ggcggctgca agtgcattga gtgcaatacc agcgagcaat
     2521 aagccagcga tggagtccgc cgtgagccag cgcgcgacgg tatccaggat aaaacaaacg
     2581 gcaaccgcgc cagcaaaggc tgtggttggt aagagccagt aacgcaaagc gacaggcacg
     2641 tctataccac tgctggcaag caacacaatg gtgagggctg ccgcgcaggc agcaccactc
     2701 gtcacaccca ataagccggg atccgccagc ggattacgga acaggccttg tgccagcgcg
     2761 ccagacagtg ccagcgttgc gccaatgagt actgcaaaca ggatacgtgg cagccggaga
     2821 tgccataaga catatgcgct gccagatagc ggctgatcac tgctttgcag cacggccagc
     2881 caatcctgcc aggtgacggt gactgctccc atagcggtag ccgcccagat gcccaatagt
     2941 agtaacaatg agagtagcca tgctggttga ctggcaaatt gctgcggcaa tccgcagatg
     3001 gttctggcca tacgggatga cggggaaatg aatgatgtca tattgatcga ctacgcggct
     3061 aagtggtgat tctgcttacg ccaactagct ttgcatggca ttgctgagcg cctggtccaa
     3121 ggctgacaag gcttgcggta aacgcgggcc aaagcccagc atgaacatcg cctcgaatgc
     3181 cacaatgcgt cgcttttgcc cggcaggcgt ctgcgccagg ccaggcaatt tcagcgcacc
     3241 atccatcccg ccaaccacac tcatcccctg ctcggtgaac agcacaatat caggctgtgc
     3301 agcaatcacc gcttctgggg ttaatggctt gaatccgtca aagccatcca ccgcattgcg
     3361 cgcgcctgca taagcaatca tggcatcagc gctagttttt ctaccaccca ccatgatttg
     3421 tgccggacta tgcgacaaaa taaacagaac ccttggtgca gatgttttac gcgcaagaat
     3481 aggcgcgcgc gcacgctgcc agtcttgctt taattgcgcc tccaactgac tggctcgtgt
     3541 agtttgctcg atcaacgccc cgacatgcct cacgcgctct atcacccctt caaatttgtc
     3601 attagcagac aacactgaca ccggcacacc agccgtcact atctgccgta ataccgctgg
     3661 aggccctgcg tcttcagtcg cgatcaccag tgtcgggctt aaagccagaa taccttcact
     3721 cgaaagggct ctggcataac ccacactggg caactttgtc gccgcttgcg gatagagcga
     3781 ggtggtatcc actcccacta actctttttc agcgcccaag gcatacaaaa tttctgtgag
     3841 cccgccacca acgctcacaa tacggcgtgc cgtttttttg cctgcttcat tgccaaaagc
     3901 aagttgtggc aaacatacca cggtcaccgc agcgcccatc cggcctaaaa acgtacgccg
     3961 tgacgatggc gtattctcat cttgtttgtt aaatacgtaa gacatgaaaa ctagaccctt
     4021 ggtaaacgtt cgatcaatgc agccaaaggc tgaccaggta gtggcaagtt catggtcagg
     4081 ccaattgatc cattttttgc tgggtcgttt tatccggggt ggtgtgcccc tgatcattac
     4141 ggtgcatcac ttgcgtcagg tcttgcccat tcaagtgata ggctttggca aaaccagata
     4201 catagctgcc gtgggcaggg gttaactcaa tcagacggaa gtccgtcagc ttgctgatga
     4261 cgtccatgaa ttgaccaaac ttttcaaaga acaaccccat gaccaaatca aatctggcgt
     4321 cctccctgct gacttcttgt gctccacatt ggaacaccag gcgctttctg gcaaacaaat
     4381 gcgcggcatc cttctccccc tcgataaaca tgacggaaca gcgaccagtg tcgcgcaggt
     4441 tagcgcaatg tgtcgccagt tcactcacat acacatagaa cttgtgttcg tgttgcacat
     4501 aagcggcata actcgcttct ggaatacctt gtgcgttggt ggttgccaac tgtatcgact
     4561 ggaactgggt aggaaaagct gcggcttctg cgccaacagc ctgtaatttt tcagtatcac
     4621 tcatgtaaaa cttctccctt tatgcatcat gctagaggca ttgatcaatt aaccacataa
     4681 tacatgataa tcattctcat ttacaacaag tgaaatatca aaatattgat aggctagcta
     4741 acaaaaggga taaaaaactg aaagaaggtg agctgactga cgcatggcgg acagtatccg
     4801 ccgactacct taacggcggg agaacagtgg ctgtacccag tagaaaaaga gtgctaccag
     4861 cacagggagc gcaatggccg cattggccag gtactcacgc gcgtcatcgt agaccagcgc
     4921 gaagatgagc ccagccaggg agaccagtgc gatcagcatc ggccagaaaa agatttgagg
     4981 cagttttttg ttcggcataa ataaatgtgt gaccatgatt gaccccttac ggacccaata
     5041 cgcatagaac gactgtgtta ataggcctca ctcagtgtaa ttcttgaacg aatatccggc
     5101 acgtactggt ggcgcttttt ccaccacagc atcagcccgg tccacaacac gacaatggtg
     5161 atgacatcca gtactgccca caaccatttc aatgtcatgc cgccataatc cccaaaatgc
     5221 aggggctgcg agatcaataa tgccactaga taccacggca attcacggct atctgttaac
     5281 gtcgccgtgc gggcatcaat gagcactggt ttaaacaggc gtttggtcag gggcgagtcg
     5341 ccgcgcatga aaatgccata atgatgcggg ctggcgtaag ccgtgcctgg gaaggcaata
     5401 aatcccaatt gcatgtcagg ctctgtctgc tgcgctacct ttacagaagc ctgtaatgag
     5461 gcaaacctgt ctggcggcgg taagtttttg tatggcgcga tcatttgcga catctggtca
     5521 aactgccagt acttaaccag caaatcagac caagcattga tcacaccggt tgctgcaacc
     5581 accaacgccc agctcagggt cacaatacca agaaaattat gcacatccag tcgcttgagc
     5641 tttttgccac gctgccgacg aatttcgcca aacgccagtt tgcgcataaa tggcgcatac
     5701 aaaacgacgc ctgacacaat agctaccagc agcaaaaaac ccatcaagcc cagaaacaac
     5761 atgcctggca acccggcgaa taagtcgaca tgcaacttga acatgatgta catgaagcct
     5821 tcctctaccg gcggctctcc aagtaccttt gccgtacgtg catccagcac cacttgcttg
     5881 aatccactat cagccgtggc ggtttttgcc atgctgatca cccattgcat cggctcgtct
     5941 tcttcacggt acatatataa gggcaccatg cctggctgcc tggctttggc tgcggccagc
     6001 atggcatcca tactggccat cggcccctgg taggcaattt caggcgtctc gatatgattg
     6061 cccagccaat cgcctatttc atgatgaaaa atcagcggca acccggtcag gcataacaac
     6121 agcataaata ccgtacagac caggctggac catttgtgta cccaagccca ggtacggata
     6181 gaagactgct tcgacatcaa agaaccttac aggatgattt cagttacggc tgtggctggc
     6241 tgacaaaacc cacttttttc atgccagcgg tttgcgcttt tgccaatact tcggccagca
     6301 cctggtaacg cgtatttttg tcagcacgga tctgtaattc tggctgctgc tgtttgccag
     6361 cctcctctga catccgcgca gcgaggtcag ccacctggat ctcactgtca ttccagaaaa
     6421 ccttgccttc agcatctact gccaagtcga tcacttctgg tttttcctct agcggctgcg
     6481 acgtcgcctg tggcaggtcg attttgactg cgtgcgtcag caatggggcg gtgataatga
     6541 agatcaccag caacaccagc atgacatcca ccagcggcgt ggtgttgatt tcactcatgg
     6601 gttgcgtgtg ctcagccgca ctgaatggat tttggcccat catacattgc cacctttatt
     6661 cgccactttg gcatgcaatt ctgtcacgtt tgctggcttg ctaccggctt taatcaccag
     6721 cggcgcacct gtagtcaaca gggtatgcaa gtcttgtgca aagctttcta gctcggccat
     6781 gatgccgcgg ttggccttga tgaatgcgtt gtaagccaac acggcaggaa tcgccaccgc
     6841 cagaccaatc gccgtcataa tcaaggcttc acccactggg ccagcgactt tatcaatggt
     6901 tgctacgcct gactggccaa tcgccgtcag cgcgtgataa atgccccaca cggtaccgaa
     6961 caagcccacg aatggggcca cgctacccac agaagccagc acggacaaac cgctttccaa
     7021 tgccgcctgc tcttgcgaca aggtacggcg caactggcgt gcgataaact catcctggct
     7081 gcaagctgcc gctaatgact ggctggcata ttgctggtga tgctgggtgg actccacagc
     7141 ggtcaatgcc agacggaatg ccggggactg cagctgcgcc tcctgcaatg cttcctgcaa
     7201 ggtggccttg cgccagaact ggcggacata agcggacaca tcacgcttgc gtttgaacac
     7261 ctgccaggat ttcacgagga taaagtacca gctcagtacc gacatggtca gtaagatcag
     7321 tgccactaac accgacacca caccgccttc ggtgataaaa gccaaaccac ttgttgtttg
     7381 cattaaaaat tccctttcac tgaaaacttg cttgatattt gaaacttgct taattctggg
     7441 tcagcgagaa tgacaagggt acattcacgt atgcgctcaa tgccttgcca cctttacgcg
     7501 caggctcaaa acgccattgc ttgacggcgt tcacagctgc cttgtccagg cgctcaaacc
     7561 cactactatt aatcacttct acagactctg gccgtccttc ggtagagacc agcactttca
     7621 tgagcaccct gccctcttca ccagcacgct gtgacaaacg agggtattca ggcgcaggat
     7681 tattgaggta agcaacccca aattttggcg gctccacttt ttcctcaacc acttcctgct
     7741 tgggcgccgc agcgggcggt gccggcttac tcggcgcagc cgccgcgggc gcctctaatg
     7801 gctgtgcaga tggctgcgta ctggcctcaa actttggctg ttctggcgtg gtttctacaa
     7861 tacgctccac aacgggtgcc ggccgctcta tgggtttagg cgtttcttta ggcttcacca
     7921 ccgttttaac gaccggttgt tttttgatga tgggcacgac ttctggcgca ggtgcttctt
     7981 cttttttagc cggcagtgta atcagggaga ctgtcatcgg ctcaggcttt ggctcaacga
     8041 tcactggctc ttcgtgcata accagccagg caatcacggc accatgcaac gccaccacag
     8101 ccagcatacc aaggccatgc cagccctggc gattgtcgcg tgacagccag tcttcagtcg
     8161 ccaataccga ccagcggaac ttgccggcca gttcacccag ttggcggcgg tgtaaactta
     8221 actcaggcaa ccgaggcgca gacgcacgct caggctttaa cgacaaccct cgcaaggact
     8281 cacgttgcag cattagcata ctcctctgac taaatcacaa aaggcaacgc cagggttgcc
     8341 atcattactc atatatttca cgcaaaaacc tctattgctt ttgctgaacc aaatcggctg
     8401 ccataaacgc ctggcatttg gtcaacaata atttattctg cttggtacgc gtcaattgat
     8461 aagcctcgcc accatgcata atcaacaaca caccagcctc gcccaacaac tgctgggcat
     8521 ctatcgtcgg catccgctgt gccgaaacac cggtcgcatt ttgcacctca ccgagtgaag
     8581 attgcaagcg gttagtcatt tgaatccatt ctaaaaaaag gccgacttac ttgcataaag
     8641 caacgcggca tcagatttca aggaacaccc ttaccaggga tgatttggcg catgaacgat
     8701 cacgcctatt tgagcaatga catgttgggg ttaggagcat tgctcaaatg ctttgcccgc
     8761 aaaccttaac gacagagcgt taaggctgct tgcagaatta aacgcggttt gcgtagtact
     8821 caaccacttg ctgcacatca atcgggaacg gcacatcttc aatcgatggc aaatggccga
     8881 tttttgcaga caccgaagct gcttctaccg ataaccactc tggcaccgtc aagctaggcg
     8941 ttgccaagga ctccaccacc atcgggatct tgcggctttt ctctttcaag ctcaccacat
     9001 cccccacttt caggcgcaca gaagcaatat tgaccgactt gccgttcagc aatacatgtc
     9061 catgattcac cagttggcgc gcagcgattc cggtaggtgc aaacccggca cggaacacca
     9121 cattgtccaa gcggcgctcc agcaatgaca tcaacttgtc acccgtcggc tcactgcctt
     9181 tacgtgcttc cagcatcagc ctacgcatct gcttttcact cacaccatag ttaaaacgaa
     9241 tcttttgttt ctctaccaac ttaactgcaa agtcagaacg gcgacgacgg gacgcctgct
     9301 gaccgtgctg accaggtggg ttaggacggt tttcaatcga cttgcgtgac agtccgggca
     9361 actctacgcc caatgcgcgc ataatcttga gtctgggtcc tgtggttctt gccatgaatc
     9421 tagtgccttt ctttgtttat ctttttatta cttattcaac tgccggttat tgcagcgtgg
     9481 ctggttgcgt tacaaaatca gtcaccgcct gctgcatggc aaattcctga tgcgcatgca
     9541 gcaatgcctg ccagtcctgt agcaattgct gggcggcaga ctggatcaat ccactctctg
     9601 catactccgg cgccgtcagg gtctgtgcca ggctgcagcc cagcaaagcc agatcctcag
     9661 aaggatttgc cgcataacgc gttgccacac aacttaaaga aaccagtact gcacttgcgg
     9721 tcggcaggtc cggatcaaaa aaatcactgg cttgggctgc gtaatcaatc tgcattgcgg
     9781 gatgactttc gttcaatcgt ttatgaaaat gataatgttt ctcatttagg atgtcaacca
     9841 ttgtttgcaa ttcgatacat acctttatat acaccttgcc atccacacca caaa
//
LOCUS       DATMSK010000008         6035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_352478,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000008 DATMSK010000000
VERSION     DATMSK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352478"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1264)
                     /locus_tag="VNZ84_00280"
     CDS             complement(197..1264)
                     /locus_tag="VNZ84_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWU65412.1"
                     /translation="MWYQETGQFITEYRLDKRFFRLKQERWAFAALLAFAFLVVPALG
                     NDYWFSAILIPLLVLSLAGLGLNLLTGYAGQLSLGSAAFMAVGAFATYNFHLRLEGLP
                     LLASLILGGLTAGGVGLVFGLPSLRIKGFYLIVATLAAQFFVQWVFTTFSWFSNNSSS
                     GVITAPPLHILGADLSTPAGRYLLTLTVVVALGWLAKTIVSGELGRKWMAVRDMDTAA
                     DVIGISVPKTKLLAFAISSFYLGIAGALWAFTYLGTVEPHGFDLSRSFQILFIIIIGG
                     MGSILGNFLGAAFIVLFPILLSQISTSLLAGAIDPGQLENFQKMIFGALIIFFLIKEP
                     NGLAKVWQTLKQRLRVWPLRF"
     gene            complement(1290..2171)
                     /locus_tag="VNZ84_00285"
     CDS             complement(1290..2171)
                     /locus_tag="VNZ84_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007246480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWU65413.1"
                     /translation="MSFFFEVLTGGLLAGVMYSLVAIGFVLIYKASGVFNFAQGSMVL
                     FAALTFVSLVERGVPFWGAFVLTLLTMAVLAWLIEWLVLRPLVNRSPITLFMATLGLS
                     YIVEGLAQAVWGAQVHALEIGINDEPYEVFGILLSQFDLFAAAIAGGLVALLGVLFNK
                     TRIGIALRAVADDQLAALAVGIHLQRLWLIVWAVAGFVGLVAGMLWGARVGVQFSLSL
                     IVLKALPVLIIGGFTSIEGAIVGGLIVGASEKLAEVFIGPVIGGGIENWFPYVLAMLF
                     LQVRPYGLFGDKAIERV"
     gene            complement(2194..2982)
                     /locus_tag="VNZ84_00290"
     CDS             complement(2194..2982)
                     /locus_tag="VNZ84_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU65414.1"
                     /translation="MSSTQPAPRPLLTLEGISLSFKGVKAITNISFAVNEGEICALIG
                     PNGAGKSSLLNVINGVYKPDEGSIRYQDALRSAMHPQWAARRGVARTFQNIALFKGMS
                     VLDNVLTGTNLKVKSSWLEQVFNVGRAPADALSQRQKAERVIAFLKLQPWRNSIVGSL
                     PYGLQKRVELARGLAAEPQLLLLDEPMAGMNADEKAEMSRFIRETNREFGTTIVLIEH
                     DIGVVMTLSDHVVVLDYGKKIGDGTPDEVRANPEVIAAYLGTKH"
     gene            complement(2979..4133)
                     /locus_tag="VNZ84_00295"
     CDS             complement(2979..4133)
                     /locus_tag="VNZ84_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWU65415.1"
                     /translation="MAETLPHLLTLQAVERPHRVAIRHKQLGLWQQSSWLTVQEAVIK
                     AAQYLQQQGFRQGDTLFLLSEPRPEALLLSLAAHWLGGVAAPLDPAFAQPQLLDLLHT
                     LRPQFVFAEAQAQVDQVLAAKPDCAVVIYADARGLAGYQHPALVSYAQVVATPEIKAI
                     ASAVTADEIAFRFYRLNTAYALEYSEVSHAELLRNGRQLVTQEALTAHEEALAARAFA
                     ASGHAKYLLSPWLLAGFTLNFPENLATRDTDRRELGPTLVAGTATTYQRLYALTQSRW
                     PLPGSWQHRVVAWALRAAKTRLPLIAAFARWLVILPLQDVLGLRRTRVPLLVGESLSP
                     EATQFFADIGILVRAWPEATAWQASQLQVIPHQSHEAHAFWGFSKWKAAI"
     gene            complement(4156..5244)
                     /gene="sfnG"
                     /locus_tag="VNZ84_00300"
     CDS             complement(4156..5244)
                     /gene="sfnG"
                     /locus_tag="VNZ84_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfone monooxygenase SfnG"
                     /protein_id="HWU65416.1"
                     /translation="MSTENIKFAYWVPNVSGGLVVSNIEQRTSWDIDYNRKLAQIAEK
                     AGFEYALSQIRFTAGYGAEFQHESVSFSHALLAATTKLKVIAAILPGPWNPVLAAKQL
                     ATIDILTQGRIAVNIVSGWFRGEFTAIGEHWLDHDERYRRSREFIEALKGIWTQDNFT
                     YRGDFYRFSNYSLKPKPLQQPHPEIFQGGSSRAARDNAAAVSDWYFTNGNTVEGIKAQ
                     VDDIRAKAAANKHHVKIGVNAFVIARDTEEEAKAVLKEIIDKANPEAVNAFGHEVKNA
                     GAASPEREGNWAKSSFEDLVQYNDGFKTNLIGTPQQIAERIVALKAVGVDLVLTGFLH
                     FQEEVAYFGEKVLPLVRELEAKTLAKAA"
     gene            complement(5272..5883)
                     /gene="msuE"
                     /locus_tag="VNZ84_00305"
     CDS             complement(5272..5883)
                     /gene="msuE"
                     /locus_tag="VNZ84_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HWU65417.1"
                     /translation="MQTTLVTDISGVMMSRPLKVTAVSGSYKLPSRTAALVQAITQKL
                     GQQIPIDLHVVELSEIAGSLVASYDPKALPVNVQQDIQAIETADLLVVATPVYRASYG
                     GLFKHLFDLVHLEALVDVPVLLAATGGTERHALIIDHELRPLFSFFQAVTLPIGVYAV
                     EADFENYQIKSEAVKARIDLAVERALPLLKHRELLSQEKLLAI"
BASE COUNT         1560 a         1741 c         1474 g         1260 t
ORIGIN      
        1 gccagccaca gccagcgatg tttgcttaaa aatttcactt aaatactcct taaaaatata
       61 aaaaccatta ttaatcgtta tttgttatat aaaaggccga acttacctca atcaatacat
      121 gttgcctctc ctttatttaa cgggattgcc cggtctaaaa tccttgacgc ttagcttcaa
      181 tactttgggt tgaggattag aaacgtagcg gccagacccg cagccgttgt ttgagggttt
      241 gccagacctt ggccaagcca ttgggctctt tgatcaggaa aaaaatgatc aaggcgccaa
      301 aaatcatttt ctggaaattt tccaattggc ctggatcaat cgccccagcc aacaagcttg
      361 tcgatatctg agaaagcaga ataggaaaca acacgataaa agccgcgccg agaaaattac
      421 ccagaatact gcccatgccg ccgataataa tgatgaacag gatttggaag gaacgtgaca
      481 aatcaaagcc atgcggctca acggtgccca ggtaagtgaa cgcccacaat gcaccggcaa
      541 tccctaagta aaatgagctg atcgcaaaag ccagcaattt agtttttggt accgaaatcc
      601 caatcacgtc ggcagcggta tccatgtcgc gtaccgccat ccacttgcgt ccaagttcgc
      661 cactgacgat ggtttttgcc agccagccca gcgccacgac gacggttaag gtcagcaggt
      721 agcggcccgc cggggtactc aaatctgcgc ccaagatgtg caagggcggt gccgtaatga
      781 cgccggatga agagttgttg gaaaaccagg agaatgtggt gaatacccat tgcacaaaaa
      841 actgtgccgc caacgtcgcc acaatcaggt aaaagccctt gatgcgcaaa ctcggcaacc
      901 cgaacaccag accgacgccg cctgccgtca acccacccaa aatcaggctg gcaagcaatg
      961 gcaagccctc tagccgcagg tgaaagttgt aggtggcaaa cgcacccacc gccataaaag
     1021 ccgctgagcc taaagagagc tggccagcgt agccagtgag caaattcaag cccaacccgg
     1081 ccagcgacag caccagcaac gggatgagga tggcgctaaa ccagtaatcg ttgccaaggg
     1141 cggggaccac cagaaaggcg aatgccagta aggccgcaaa cgcccagcgc tcctgcttga
     1201 ggcggaaaaa ccgtttatcc agcctatact cggtaataaa ttgtcctgtt tcttgatacc
     1261 acatgctaag cctgcctgct ctctattttc taaacacgtt caatcgcttt gtcgccaaac
     1321 aacccataag gcctgacctg cagaaacaac atcgctaaca cgtaagggaa ccagttttca
     1381 atgccgccgc caatcacggg gccaatgaaa acctcggcca gtttttcgct ggcgccgacg
     1441 atcaaaccac cgactatcgc accttcaatc gaggtaaaac cgccaataat cagcacaggc
     1501 aacgccttga gcacaatcag cgacagtgag aactgcacac cgacgcgtgc gccccacaac
     1561 atgccagcga ccaggccgac aaaaccggcc accgcccaca caatcagcca caagcgttgc
     1621 agatggatgc ccaccgccaa cgctgccagt tggtcatctg ccacggcgcg caaggcaatg
     1681 ccaatgcggg ttttattgaa gagaaccccg agcagggcga ctaggccacc ggcaatggcc
     1741 gccgcgaaca aatcaaattg cgacagcaaa ataccgaaca cttcatacgg ctcgtcattg
     1801 atgccaatct ccagcgcatg cacttgtgcg ccccacacgg cttgtgccag cccctcgacg
     1861 atataactca ggcccagggt cgccatgaac aaggtaattg gcgaacggtt caccagcggc
     1921 cgcaacacca gccattcaat cagccaggcc agcaccgcca tggtcagcaa ggtcagcaca
     1981 aaggcacccc agaacggcac gccgcgttcg accaggctga caaaggtcaa tgccgcgaac
     2041 agcaccatag agccctgggc aaaattaaac acgccagagg ccttgtagat cagcacaaag
     2101 ccgatggcga ccagcgaata catcacaccc gccagcaagc caccggtcag gacttcgaaa
     2161 aagaaactca tctcaattga tccttgtatt gcctcagtgt ttggtgccca ggtaagcggc
     2221 aatcacctcc gggttggcgc gcacttcatc gggcgtgcca tcgccaattt tcttgccata
     2281 atccagcacc accacatggt ctgacagtgt catcaccacg ccgatatcat gctcaatcag
     2341 cacaatcgtg gtgccaaact cgcggttggt ttcacggata aaccggctca tctccgcctt
     2401 ttcatcggcg ttcatgccag ccatcggttc atccagcaac agcagttgag gctcggcagc
     2461 cagcccacga gccagctcga cgcgcttttg caagccatag ggcaaactgc ccacaatact
     2521 gttacgccag ggttgcagtt tgagaaaagc aatcacacgc tctgcttttt gccgttgtga
     2581 caacgcatcc gcaggtgcgc gacctacgtt aaatacctgc tccagccaac tgcttttgac
     2641 cttgaggttg gtgccggtca gcacgttgtc gagcactgac atgcctttaa acaaggcaat
     2701 attctgaaat gtgcgtgcca caccgcgtct ggcggcccat tgcggatgca tggcactgcg
     2761 taacgcatcc tggtagcgga tgctgccttc atcgggttta tagacgccat taatcacatt
     2821 gagtaatgag cttttgcccg cgccattcgg gccgatcaaa gcgcaaattt cgccctcatt
     2881 gacggcaaaa ctgatattgg tgatggcttt cacgccctta aaagacaatg aaataccctc
     2941 taaggtcaac aatggtctag gtgcaggttg ggtagaactc atatcgcagc cttccatttg
     3001 gagaatcccc aaaaggcatg ggcctcatgt gactgatgcg gaatgacttg caactggctg
     3061 gcttgccagg cagtggcttc cggccaggcc cgcacgagga tcccaatgtc agcaaaaaac
     3121 tgtgtcgcct cgggtgacaa ggattcgccc accagcaagg gcacacgggt acgccgcaga
     3181 cctagcacat cctgcaaggg cagtataacc agccagcgtg caaaggcagc gattagcggc
     3241 agccgggttt tggctgcacg caaggcccaa gccacaaccc gatgctgcca gctaccgggc
     3301 aagggccagc gggattgggt gagggcatac aggcgctggt aagtcgttgc cgtacctgcc
     3361 accagcgtag ggccaagctc acgcctatcg gtgtcacgtg tcgccaggtt ctctggaaaa
     3421 ttgagggtaa agcccgccaa tagccacggc gaaagcaggt atttggcatg gccgctggca
     3481 gcaaatgcgc gggcggccaa ggcctcttca tgcgcagtca gtgcctcctg agtgaccagt
     3541 tggcggccat tcctgagtag ttcggcatgg ctcacctcac tgtattccag cgcataggcg
     3601 gtattcaaac ggtaaaagcg gaaggcaatt tcatcagccg ttaccgcaga cgcaatggct
     3661 ttaatttctg gcgtcgccac cacctgtgca tagctaacga gtgcaggatg ctgatacccg
     3721 gccaagccgc gcgcatcggc ataaatcacc acggcgcaat ccggcttggc agccaatact
     3781 tgatccacct gtgcctgtgc ttcggcaaac acaaactgcg gccgtagggt atgcaacaaa
     3841 tccagtaact gcggttgcgc aaaggccggg tccagcggtg cggccacgcc gcccagccaa
     3901 tgggcagcca gcgacaacag caaggcctcg ggccgcggct cactcaacaa aaataaagtg
     3961 tcgccctggc gaaaaccttg ttgctgcaaa tactgcgcgg cctttatgac ggcctcttgc
     4021 accgtgagcc aagaagactg ttgccagagg cccagctgtt tatgcctgat tgcgacacga
     4081 tgcggccgtt ccacggcttg caacgtcagc aagtggggta aagtctctgc cattgcctgc
     4141 ttcctgccgt ttcagttaag ccgctttggc caaggtttta gcttccagct cacgcaccaa
     4201 tggcagcact ttttcaccaa aataagcgac ctcttcctgg aagtgcaaaa agccagtcaa
     4261 caccagatca accccaacgg ccttcaaagc gacaatacgc tcggcaattt gctggggtgt
     4321 gcctatcaaa ttggttttaa agccatcgtt atactgcacc aggtcttcaa agctcgattt
     4381 cgcccagtta ccttcacgct ctggactggc agcaccggca tttttcactt catgaccaaa
     4441 cgcattcacc gcttcagggt tggctttatc aataatttcc ttcaataccg ctttggcttc
     4501 ttcttcggta tcgcgggcaa tcacaaaggc attgaccccg attttgacgt gatgcttatt
     4561 cgccgcagcc ttggcgcgga tatcgtccac ctgggccttg atcccctcta ccgtattgcc
     4621 gttggtgaaa taccagtctg agacggcggc cgcattatcc cgcgcggcac gtgaactacc
     4681 gccctggaaa atttcaggat gtggctgctg caaaggttta ggcttgagtg agtagttact
     4741 aaagcggtaa aaatcgcctc tgtaagtaaa gttatcctgg gtccagatcc ccttgagtgc
     4801 ctcaatgaac tcgcgcgaac ggcggtagcg ctcgtcgtga tccagccaat gctcgccaat
     4861 cgccgtaaac tctccacgga accagccgga gacgatattc accgcaatcc gtccctgggt
     4921 gagaatatca atggtcgcca attgcttggc ggccagcacc ggattccagg ggccaggcaa
     4981 aatggcggca atcactttta gcttggtggt cgccgccagc aaggcatgcg aaaaagatac
     5041 cgactcatgc tggaactcgg ccccataccc cgcagtaaaa cggatttggc tcaaggcata
     5101 ttcaaatccg gccttttcgg caatttgtgc cagcttgcgg ttgtaatcga tatcccaact
     5161 ggtgcgctgc tcaatgttac tgaccaccag gccaccactg acattaggca cccaataagc
     5221 aaatttaatg ttttctgtac tcatctttta tctctccaaa agtcactgga tttaaatggc
     5281 taataatttt tcctggctca acagctcgcg atgcttgagc aaaggcagcg cacgctctac
     5341 cgcaaggtca atgcgcgctt tcaccgcctc actcttgatt tgataatttt caaaatccgc
     5401 ttcaaccgca tatacgccga ttggcaacgt gactgcctgg aagaaactga ataatggacg
     5461 cagctcatga tcaataatca aggcatggcg ctctgtcccg ccagtcgcgg ccaggagcac
     5521 aggcacatcc accaaggctt cgaggtgcac cagatcaaac aaatgcttga ataaaccacc
     5581 ataagaagcg cggtaaaccg gagtcgccac caccagcaaa tcagcggttt caatcgcctg
     5641 gatatcttgt tgcacgttga ctggcaacgc tttgggatcg taggaagcga ccaggctgcc
     5701 ggcgatttcg cttaactcca ccacgtgcag atcaatcggg atttgctgcc ccaacttttg
     5761 cgtgatcgcc tgcaccagcg cagcggttct agaaggtaat ttatagctgc cggagacggc
     5821 agtgactttt aaaggacggc tcatcatgac tcctgaaata tcggtgacta atgtcgtttg
     5881 cacgaaaact cgcaaacgac tcgcttgcta gagtattagc accatccatg ccagggaata
     5941 atatggttat atttcaatca cttatataaa aaaatccctt ttaataggtg ttggattcca
     6001 gcgctagttg ctgatcaact gctgctaaat cagca
//
LOCUS       DATMSK010000009         5487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_376035,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000009 DATMSK010000000
VERSION     DATMSK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376035"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="VNZ84_00310"
     CDS             <1..1785
                     /locus_tag="VNZ84_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylosucrase"
                     /protein_id="HWU65418.1"
                     /translation="QLAHLISVLAEQYRKRSPALKEQDLLREQDHNWFMHQKWVGMAL
                     YCEGFAGTLADMQDKLPYLQELGVNMVHIMPICDCPAENNDGGYAVRDYKKIDPRYGT
                     QKDLDQLITLIQQREMLLALDVVVNHTSDEHEWAKKAKQGDATYQDYYYMFDDRTLPD
                     IYESAMPEVFPEQAPGSFTFNEDTQKWVMTVFNHYQWDLNYSNPAVFIEMLDVILFWA
                     NKGVDILRLDAVAFLWKKMGSTCQNEREAHLILQLMKDCCQVAAPGVLFIAEAIVSPT
                     EIIKYFGEDAINAKECEIAYNATLMALLWDAVATKQTQLLYKGIEGLPEKLERATWLN
                     YIRCHDDIGLGFSDESIIAAGFDPVSHRRFLVDYYTGNYADSNAKGFAFGVNKKTGDA
                     RISGALASLAGLETALQKQDAAAIENALQTITLLHNIIMAFGGMPLLYYGDAVGTLND
                     DAYLLDSSKMNDNRWAHRPTLNWEQMDRRLQKGTIEQRIFSAIQHLISVRKEISAFAD
                     LNDRTLIALENPHVIAFSREVSSGMHARVLVIANFDVHAHAVPIQPFRQKGWFKEAMV
                     TDLCSGQTIPIANEALNIPKLSAYWLST"
     gene            1809..2678
                     /locus_tag="VNZ84_00315"
     CDS             1809..2678
                     /locus_tag="VNZ84_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWU65419.1"
                     /translation="MTWLTHLGIENTTEVLILIKTLLRIFLIVAMAYAFSYLSKKIIS
                     RLRDHLIVRSNNNIEEIKRMNTLSMVLRYIVTTVIMAITVVEILHELGISIAPVLAAA
                     GVVGLAVGFGAQSLVKDYFNGFFLLLENQIRKDDVVEIAGKAGLVEEITLRYIKMRDY
                     EGNVHYVPNGQITTVTNRSRDFAYAVMDIRVGYDENIAKVMQIMHEVGAAIVIDEALK
                     DKVLDKLEIAGVDNLAESAVIIRCRIKVRPLEQWTIKREYLKRIKNAFDAQEIEIPFP
                     HLTVYQGQGQLAA"
     gene            complement(2717..3190)
                     /locus_tag="VNZ84_00320"
     CDS             complement(2717..3190)
                     /locus_tag="VNZ84_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65420.1"
                     /translation="MDTFSHALWGYGLFGFRKYPKFAALMGAMPDLISFGALFVMRLL
                     DGTYTWGKPALATLPSWIYPAYTVGHSFVIAFAVIGLVAIWHKDIAFAMLGWPFHITL
                     DFPFHSLQYFPTPMFWPISDFRIDGIPWSHWYIWFPNVAGIIILLAYRYRQKRKA"
     gene            complement(3209..4561)
                     /gene="glmM"
                     /locus_tag="VNZ84_00325"
     CDS             complement(3209..4561)
                     /gene="glmM"
                     /locus_tag="VNZ84_00325"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWU65421.1"
                     /translation="MSKQYFGTDGIRGRVGESVITPEFVMKLGYAAGRVLANHEQLPA
                     GNRPTVLIGKDTRISGYMLEASLEAGLSAAGVDVLLTGPMPTPAVAYLTRALRAQAGI
                     VISASHNPYYDNGIKFFSAQGTKLADDIEYAIEAELAKPMQVMESAQLGKAKRVDDAA
                     GRYVEFCKSTFPNHLDLRGLKIVLDCAHGATYHVAPDVFHELGADVIAIGNQPDGLNI
                     NEKVGSTHPEALQKAVVEHQADLGIAFDGDGDRVMMVDGAGNLLDGDQLLYIIAMGLY
                     AKGKLKGGVAGTLMTNLALEHALQKHQIPFGRAKVGDRYVLELLQDRNWKLGGENSGH
                     ILTLDKHTSGDAIIAALQVLQSVRESGKTLQQLGADLTLYPQVLINVTTKQKLDLNQA
                     AVQSAVTQAEQVLQGEGRVLLRASGTEPKIRVMVEGLNKNLVQSQAEELAAVIRKLAA
                     "
     gene            complement(4605..5429)
                     /gene="folP"
                     /locus_tag="VNZ84_00330"
     CDS             complement(4605..5429)
                     /gene="folP"
                     /locus_tag="VNZ84_00330"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWU65422.1"
                     /translation="MLFHCGRFRLHFDSPKVMGIVNVTPDSFSDGGQFNQVDAAVAHA
                     LELIAQGADVLDIGGESTRPNATPVTLDEELRRVIPVIEALVAHDIPVPISIDTYKPA
                     VMRAAIAAGASIVNDVRGLQEDGALEAVVNSDAGVCIMHMQGTPQTMQIAPHYEDVVA
                     EVKRFLRTQRDTCLAAGIANERIMLDPGFGFGKTRAHNIALAKGLPQLLDLNCPLLVG
                     LSRKSVLGQMTGNDVDARLYASVAAAVISAMQGAHLLRVHDVKATAEALKVVAALQ"
BASE COUNT         1423 a         1404 c         1327 g         1333 t
ORIGIN      
        1 caactggcac atttaatttc ggtgctggcc gagcaatatc gcaaacgctc tcccgcactg
       61 aaggagcagg acttgttacg cgagcaagac cataactggt tcatgcatca aaaatgggtg
      121 ggcatggcgc tctattgcga aggctttgcc ggcacgctgg ccgacatgca ggacaaactg
      181 ccttatttgc aggagctggg tgtgaatatg gtgcatatca tgccgatctg tgactgcccg
      241 gcagaaaaca atgatggtgg ctatgccgtg cgtgattaca aaaaaatcga tccgcgctat
      301 ggcacacaaa aagatttgga ccagttaatc accctcatac aacaacgcga aatgttactg
      361 gcattggacg tcgtggttaa ccacacctca gatgagcatg aatgggccaa aaaagccaaa
      421 cagggagatg ccacctacca ggactactac tacatgttcg acgaccgcac gctgccggac
      481 atttatgaaa gtgccatgcc tgaagtattc cccgagcagg cgcctggtag ttttaccttt
      541 aatgaagaca cgcagaaatg ggtgatgacg gtgttcaacc attaccagtg ggacttgaac
      601 tacagcaatc cggccgtgtt tatcgaaatg ctggatgtca ttttgttttg ggccaacaaa
      661 ggcgtagata tcttgcgcct ggatgcagtg gcttttctct ggaaaaaaat gggcagcacc
      721 tgccaaaatg aacgtgaagc ccatttgatc ttgcaattga tgaaagattg ctgccaggtc
      781 gcggcacctg gtgtgctctt tattgctgag gcgattgtct ctccgactga aatcatcaag
      841 tattttggcg aagatgccat aaacgccaaa gaatgtgaaa tcgcctataa cgcgacgctc
      901 atggcgttgc tgtgggatgc ggtggccacc aaacaaacgc agttacttta taaaggcatt
      961 gaaggcttgc cggagaaact ggaacgtgcg acctggctga attatattcg ctgtcacgat
     1021 gatatcggtt tgggattcag tgatgaatcc attattgccg ctggttttga cccggtatcc
     1081 caccgccgct ttctggtcga ttattacact ggcaattatg ccgactcgaa tgccaaaggg
     1141 tttgcctttg gcgtcaataa aaaaacgggc gatgcgcgaa tttcaggcgc cctcgcctcc
     1201 ctcgccggac tggaaaccgc attgcaaaag caagacgcgg cggcgattga aaatgccctg
     1261 cagaccatca cgcttttgca taacatcatc atggcttttg gtggcatgcc cttactttat
     1321 tatggggatg ccgtgggcac gctcaatgac gatgcctacc tgcttgattc ttccaaaatg
     1381 aatgacaatc gttgggcaca ccgcccgacc ctcaactggg agcaaatgga taggcgcctg
     1441 caaaaaggga cgatagaaca acggatattc agtgccatcc agcatttaat cagcgtgcgc
     1501 aaagaaatct ctgcgtttgc tgacctcaac gatcgtacgc tgatcgcgct ggaaaatccg
     1561 cacgtcatcg cttttagccg tgaagtgtct tccgggatgc atgcgcgtgt cttggtgatc
     1621 gccaactttg atgtacacgc acacgccgtt cctatccaac cattcaggca aaaaggctgg
     1681 ttcaaagagg cgatggtgac tgacttgtgt agcggacaaa ccattcctat tgcaaacgaa
     1741 gcattaaaca tccccaaact gagtgcctac tggctctcaa cctaatttta caaaggagca
     1801 tccaccacat gacttggctg acacatctgg gcatcgaaaa cacgaccgag gttttgattt
     1861 tgataaaaac cctgctgcgt atctttttga tcgttgccat ggcttatgca tttagctact
     1921 taagtaaaaa aatcattagc cgcttgcgtg atcacctgat tgtcagaagc aataataaca
     1981 tcgaggaaat caagcgtatg aatacgctaa gcatggtgtt gcgctacatt gtgacgaccg
     2041 tcattatggc cattaccgtc gtagaaatcc tgcatgaact tggcatctcc atcgcacctg
     2101 tattggcggc tgccggggta gtcggcctgg cagttggttt tggcgcgcaa agcctggtca
     2161 aggattattt caatggattc tttttattgc tggaaaacca gattcgtaaa gatgatgtgg
     2221 tcgagattgc tggtaaagcc ggcctggtgg aagaaattac cttgcgctat atcaaaatgc
     2281 gtgattacga aggcaatgtc cattatgtgc caaatggcca gattacaacg gtaaccaatc
     2341 ggtcgcgcga ctttgcttac gccgtgatgg atatccgcgt aggctacgat gaaaatatcg
     2401 ccaaagtgat gcagatcatg catgaggtgg gtgcggccat tgtcattgat gaagccctga
     2461 aagataaggt attggataag cttgaaattg ccggggtaga caacctggca gaatcagcgg
     2521 tgattatccg ttgccgcatc aaggtccggc cactggagca atggacgatc aagcgcgaat
     2581 atcttaaacg gattaaaaat gcatttgatg ctcaggaaat tgaaattccc tttccacatc
     2641 tcaccgttta tcaaggccag gggcaactgg cggcttaata caaaacgggc gataagcccg
     2701 ttttttatgt cttagttcac gctttgcgtt tttgcctgta tcgataagcc agcaggataa
     2761 tgatgccagc tacattcggg aaccagatat accagtgaga ccaagggata ccatctatcc
     2821 tgaaatctga aatcggccag aacatcggcg ttgggaagta ttgcaggcta tggaacggaa
     2881 aatcgagggt aatgtggaac ggccagccca gcatggcaaa cgcgatatcc ttgtgccaga
     2941 tcgccaccag gccaatcacg gcaaatgcaa tcacaaagct atgccctacc gtgtatgcag
     3001 gataaatcca gctgggcaat gttgccagtg caggcttacc ccaagtgtag gtgccatcca
     3061 atagtcgcat cacaaacaag gcaccaaaag aaatcaggtc tggcatggcc cccatgagtg
     3121 ccgcgaattt tggatatttg cggaatccaa acagtccgta tccccataag gcgtgactaa
     3181 aggtatccat ggaaggctcc cgataaggtt aagcagccag tttgcggatc acggcagcca
     3241 gctcttcagc ttgagattgc accaagtttt tattcaagcc ctccaccatg acgcggatct
     3301 tgggttcggt accggaggca cgtaacaaca cacgcccttc tccttgtaac acttgttcag
     3361 cctgggtcac tgcggattgc acagcagcct ggttcaagtc tagcttttgt ttagtggtca
     3421 cgttaatcaa cacttgcgga tacagggtta aatcggcccc taattgttgc aaggttttgc
     3481 cgctctcccg cacggattgc aacacttgta atgctgcaat gatggcatcg ccactggtgt
     3541 gtttatccag ggtgaggata tggcctgaat tctcaccacc gagtttccag ttccggtctt
     3601 ggagcagctc cagcacatag cggtcaccta ctttggcgcg accaaatgga atctggtgtt
     3661 tttgcaaggc gtgctctaat gccaggttgg tcatcaaagt gcccgcaacc ccccctttga
     3721 gtttgccttt tgcgtataat cccatggcaa tgatatagag cagctggtca ccatcgagta
     3781 ggttgccagc accatcaacc atcataacgc ggtcaccgtc accatcaaag gcaatgccta
     3841 agtctgcctg gtgctcaacc acggcttttt gcagggcttc aggatgtgtt gaacccactt
     3901 tttcattgat atttaagcca tccggctggt taccgatagc aattacatcc gcacctaatt
     3961 catgaaagac atccggggcg acgtgataag tggcaccatg tgcacaatcc agcacaattt
     4021 tcaggccgcg caaatccagg tggttaggga aagtgctttt acagaattcc acataccgac
     4081 cagccgcatc gtctacgcgc ttagccttgc ctagctgtgc cgactccatg acttgcatgg
     4141 gtttcgccag ctctgcctca atggcatatt caatatcatc agccagtttg gtcccctgtg
     4201 cagagaaaaa tttgatgcca ttgtcatagt atgggttatg cgaagccgag atcacaatgc
     4261 cggcctgtgc acgcagggca cgcgttaaat atgccacggc aggcgtcggc attggcccgg
     4321 tcaacaacac atccacaccg gccgctgata gtccggcctc cagtgaggcc tccagcatat
     4381 aaccggaaat acgcgtatct ttacctatga gtacggtcgg acggttaccc gcaggtagtt
     4441 gttcatgatt ggctaatacg cgaccggcag catagcccag cttcatgaca aactcagggg
     4501 taatgactga ctccccaaca cgtccgcgga taccatctgt tccaaaatac tgtttactca
     4561 tcgctctaat ctttctctca ctcattgtgc tcttgttatt ttgtttactg caatgccgca
     4621 acgactttaa gcgcttctgc agttgccttt acatcatgta cacgcaacaa gtgcgctccc
     4681 tgcatggcac tgatgacagc agcagcaacg ctggcataca ggcgtgcatc cacatcgttg
     4741 cctgtcattt gtcccaggac cgatttacgt gacaatccca ccagcaacgg gcaattgagg
     4801 tccagtaact gcggcaagcc tttcgctaag gcaatattat gcgccctggt tttgccaaag
     4861 ccaaaaccag gatccagcat aatccgctca ttcgcaattc ccgctgccag gcaggtatca
     4921 cgctgcgtgc gtaaaaaacg ctttacctcc gcaaccacat cctcataatg tggggcgatt
     4981 tgcatggtct gcggggtgcc ctgcatatgc atgatacata cacctgcatc gctatttact
     5041 accgcctcca gtgcgccatc ctcctgcaag ccacgtacat cattcacaat actggcccct
     5101 gcagcaatgg cagcacgcat cacggccggc ttatatgtat cgatagaaat aggtaccgga
     5161 atatcatgtg caaccagtgc ctcaatcaca ggaatcactc gccgtaattc ttcatctagt
     5221 gtgacgggag ttgcgttagg ccgggtagac tctccaccaa tatccaacac atctgcaccc
     5281 tgcgcaatta attcaagtgc atgtgcaacc gcggcatcta cctggttaaa ctgtccccca
     5341 tcagaaaaag agtctggtgt gacattcacg atccccatca ctttggggga atcgaaatgg
     5401 aggcgaaagc gaccgcagtg aaacagcata agatagtgag ttccccaaaa aacaaaggct
     5461 ggcaaatgcc agccttgtta ttttaac
//
LOCUS       DATMSK010000010         5050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_141583,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000010 DATMSK010000000
VERSION     DATMSK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141583"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VNZ84_00335"
     CDS             complement(<1..389)
                     /locus_tag="VNZ84_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HWU65423.1"
                     /translation="METTQYFNKTEKDTLLECLLYICRHNRLATTRDALTSGLPLEDG
                     KLNPELFKRSAQRLRLDVSVKQEAIPVLLLQPQVNITLLLNNNRACQLLKVDDSQHFA
                     QVVFPDLSPEPVTMSMADLVAQYTGYA"
     gene            complement(527..2455)
                     /locus_tag="VNZ84_00340"
     CDS             complement(527..2455)
                     /locus_tag="VNZ84_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU65424.1"
                     /translation="MSLVKQIRIAISVIILMVAAGCLFLSVYDDHRFMAEQLQKKNND
                     NANGLAITLSNIPKDSVTVELIISAQFDMGHYRRIAIISPENKVMIERVNHNPHSVAP
                     AWFQSVFSVSIQPGIANIQSGWQQYGTLVLESEPSLAYDQLWETSRHAVLWTLIVAFM
                     SYVVSGFWLKKILKPLDDVIAQAEALGERKYITIAEPATEEFRKLVRTMNTLSDRVKS
                     MVTAESERLNTFNQQINYDEVTGLMNQSHFTNTASVALKNEGFVEGMFIMVQLRNLPE
                     IDKQLGYSVTNQLIKKVADTVQQQMGNYHDVVCGRLSGGVFGIFSREPLDDFAVTTEL
                     KLMLEKLSAAQQLLQFQFVLTHTKTKKGDDFVDVHRVMQFILDLSEDQAAVPMRVMNA
                     NSIVTSRNNYLNQWKEQFLLAIQLKQIKLVRFPVMKRDQHLYHYECPLRLRIAEGEQW
                     LAAGAFIDWASQLDMIQTLDGLALEYAVDMLATNNDNLSVNVSAAAMRSDEYRDKLQA
                     LILTVNHPARLSFEVTEEAAFGDFVKFREFVHLVKSLGCMIGVEHVVTRLAQLGDLHE
                     LGLDYIKFDASLIRDIHLRPQQQSLMRGLCMMVRTMGIVPIAEGVQDQQELDTLNAIG
                     IEAVTGPFVQEDAQFGVL"
     gene            complement(2571..3245)
                     /locus_tag="VNZ84_00345"
     CDS             complement(2571..3245)
                     /locus_tag="VNZ84_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HWU65425.1"
                     /translation="MRRHVHSPLVRLLTGICLALSLLWTPILADDYVLAMLEKIRSRY
                     GEEAYQNVMRLNELFTQISGASEMTKVNMVNDFTNQHVLFVDDFSLWGVQDYWATPLE
                     TFGKGAGDCEDFSIAKYTLLKKLGVSQDKLRLTYVRARMSSGAILPHMVLTYYATPTS
                     DPLVLDNLNFELLPASRRGDLSPIFSFNDKGLFVANNPNMRAGSPSNISKWRDVLTRM
                     RQDGLE"
     gene            complement(3315..3437)
                     /locus_tag="VNZ84_00350"
     CDS             complement(3315..3437)
                     /locus_tag="VNZ84_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65426.1"
                     /translation="MSKKLLDSKDDILPNPASLVFVENGFLLMTMGDAQAEVVD"
     gene            3436..4809
                     /gene="purB"
                     /locus_tag="VNZ84_00355"
     CDS             3436..4809
                     /gene="purB"
                     /locus_tag="VNZ84_00355"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWU65427.1"
                     /translation="MTSLTTLNALSPLDGRYQSKLDPLRPFFSEYALIKYRALVEVAW
                     LKALSAAPELSEIAPFSAETVAELDAAIATFSEADATQVKAIEARTNHDVKALEYWLK
                     EKFDGNHEIKKASEFIHFACTSEDINNLSHALMLKSARDSVMLPNLQTVVGRLIELAQ
                     ALADQPMLSRTHGQTASPTTMGKELANVVYRLQRQLKQLQTNEVLGKINGAVGNFNAH
                     LSAYPDFDWESFAKRFVESLGLTYNPMTIQIEPHDYMAELYDTLSRINTILIDLNRDI
                     WGYISVGYFKQKVKAGEIGSSTMPHKVNPIDFENSEGNLGLANAVLRHLAEKLPISRW
                     QRDLTDSTVLRNMGVAFGYALLGYDSCLRGLNKLEVNPQRLLEDLDNSWEVLAEPIQT
                     VMRRYGIENPYEQLKELTRGKGGINKQSLHAFIETLQIPADAKKALLALTPATYTGKA
                     SQLVKHI"
BASE COUNT         1380 a         1324 c         1101 g         1245 t
ORIGIN      
        1 aaggcgtagc cggtgtattg cgcgaccaag tccgccatgc tcattgtcac aggctctggc
       61 gacagatcag gaaaaaccac ttgggcaaaa tgctggctgt catcaacctt taataactga
      121 caggcacgat tgttattgag caacagtgtg atattcacct gcggttgcag tagcaataca
      181 ggaatggctt cttgcttaac actcacatcc aggcgtaaac gttgagccga gcgcttaaac
      241 aattcagggt tgagcttgcc atcttccaag ggcaacccgg aagttaaagc atcacgggtc
      301 gtcgccagac ggttgtggcg gcaaatataa agtagacact ctaaaagggt gtctttctcg
      361 gttttattaa aatattgcgt cgtttccatg gcagatgact tgttattatc cttatccgag
      421 caaaatcagg cgctgattgg ccgatgcttg attctagcga ccatcaagca aaatcaaaac
      481 ggtgagtata tggcataaca cccccgaatt acaaaaattg acactcttac aacaccccaa
      541 attgggcgtc ttcctgtacg aatgggccgg tcacagcttc aatgccaatc gcattcaggg
      601 tatcaagctc ttgctggtct tgtacgcctt cagcaatcgg cacaatcccc atcgtgcgta
      661 ccatcatgca caaacctcgc atcagcgatt gctgctgtgg acgcagatga atatcgcgaa
      721 tcagtgaggc atcaaacttg atgtaatcca atccaagctc atgcaaatcg cccagctgtg
      781 ccaatcgagt gaccacatgc tcaacaccta tcatgcagcc cagggatttc acgagatgta
      841 caaactccct gaatttcaca aaatcgccaa aagcagcttc ttcagtcact tcaaaactta
      901 agcgggctgg atggttgact gtcaatatca atgcttgcaa cttatccctg tactcatcac
      961 tgcgcatggc ggcagcagag acattcacac tcaggttatc attatttgtc gccagcatat
     1021 cgaccgcgta ttccaacgcc aaaccgtcca gtgtttgaat catatcgagc tggctcgccc
     1081 aatcgataaa tgcgccagca gccaaccatt gttccccttc agctatacgc aaacgtaatg
     1141 ggcactcata gtggtacaaa tgttgatccc gtttcatcac cgggaagcga accagtttga
     1201 tctgcttgag ctgaatggcg agcaggaatt gctctttcca ttgattaagg taattattcc
     1261 ttgaggtcac aatgctgttg gcattcatga cacgcatagg cacagcagcc tggtcttcag
     1321 agagatcaag aataaactgc atcacgcgat ggacatctac aaagtcatcg ccttttttgg
     1381 tttttgtgtg cgtgaggaca aactggaact gcagtagttg ttgcgccgcg ctaagcttct
     1441 ccagcatcag tttcaactcg gttgtcactg caaaatcatc cagcggctcc cggctgaaaa
     1501 taccgaaaac acctccggaa agacgtccac aaacgacatc atggtaattt cccatctgct
     1561 gctgaacagt atctgccact tttttaatca gctggtttgt cacgctatag cccaattgct
     1621 tgtcaatttc cggcaaattg cgcaattgca ccatgataaa catgccttct acgaaccctt
     1681 cattcttcag ggcgactgac gcagtattgg tgaagtgtga ctgattcatc aaaccagtga
     1741 cttcatcata attaatctgc tggttgaaag tgttcaggcg ctctgactcg gccgtgacca
     1801 tggatttcac gcggtcggac agcgtattca tggtccgcac cagcttccgg aattcctctg
     1861 tcgccggttc ggcaatcgta atgtatttgc gctcacccaa tgcctcagct tgcgcaatca
     1921 catcatccag tggcttgaga attttcttca gccaaaagcc tgaaaccaca tagctcatga
     1981 aagccacaat caatgtccac aacaccgcat gacgtgaagt ttcccataac tggtcatacg
     2041 ccagcgacgg ctcactttcg agcaccaaag tgccatattg ctgccaacca gattggatat
     2101 tggcgatccc cggctggata ctgacagaaa atactgattg gaaccaggca ggtgccacag
     2161 aatgcgggtt gtgattaacc cgttcaatca tgaccttatt ctccggagaa ataatggcaa
     2221 tccggcggta atgccccata tcgaactggg cactgatgat caactccaca gtgacactgt
     2281 ctttgggaat attggaaagt gtgattgcaa ggccattggc attatcgtta ttttttttct
     2341 gcaattgttc agccataaat cgatggtcat cgtatacgct taagaacaag catccagccg
     2401 ccaccattaa aataatgaca gaaatcgcaa ttcgtatttg ttttaccaaa gacatttgaa
     2461 agccctctta ctttcctgga gtagatccgg cactagcccg atgtcaacgc aagccatgct
     2521 tagcctagca ttaacccccc ataataacca cacccctaac aggtttttaa tcattccagc
     2581 ccatcttgcc gcatccgcgt caacacatca cgccatttgg aaatattgga aggagaacct
     2641 gcccgcatat ttggattatt tgcaacaaac aaccctttat cattaaaact gaatattggt
     2701 gacaaatcgc cacgcctgga cgctggcaat aattcaaaat taaggttatc caataccaat
     2761 ggatcactgg ttggcgttgc ataataggtc aacaccatat gtggcaggat agcgccactg
     2821 gacatgcgtg cccggacata ggtcaaccgc aatttgtctt gcgacacacc aagctttttc
     2881 agcaaggtgt acttggcaat actaaaatcc tcacaatcac cagcaccttt gccaaacgtc
     2941 tccagcgggg tggcccaata atcttgcacg ccccacaggc tgaaatcgtc gacaaataat
     3001 acatgctggt tggtgaagtc attcaccatg ttcactttgg tcatttcgct ggcgccacta
     3061 atctgcgtaa acaattcatt caggcgcatg acgttctggt aagcctcttc gccgtagcgc
     3121 gacctgatct tttccagcat tgccagcaca taatcatccg ccaggatcgg tgtccatagc
     3181 aggctcaatg ccaggcatat gcctgtcagc agtcgaacca aaggtgaatg tacgtggcgt
     3241 ctcatgtgaa atgtattccc agtgagcgat tatgtcatgg caaaatcaag cacaatttac
     3301 caaacaaagt ggttttaatc gacaacttca gcctgcgcgt ctcccattgt catcaataga
     3361 aaaccatttt caacaaagac aagcgaggct ggattgggta aaatatcgtc ttttgaatca
     3421 agaagttttt tactcatgac gtctctcacc acgctgaatg cactatcgcc actggatggc
     3481 cgctatcaat caaaactcga tcctttgcgc ccgtttttta gtgaatatgc actgattaag
     3541 tatagagcac tggttgaagt cgcatggtta aaagcactga gtgctgcacc tgaactgtcc
     3601 gaaattgccc cgttcagcgc agaaacagtc gccgaactgg atgccgcgat cgcgacattt
     3661 agcgaggcag atgccaccca ggtaaaagcc atcgaggcgc gcactaatca cgacgtcaag
     3721 gcactggaat attggctaaa agagaaattc gacggtaacc atgaaatcaa aaaggccagc
     3781 gaatttatcc actttgcctg tacatcagaa gacattaata acctgtcaca tgcactgatg
     3841 ctaaaatctg cacgcgacag cgtgatgtta cctaacttgc agactgtggt tggccgcctg
     3901 atcgaactgg cacaggcatt ggccgatcag cctatgttat cccgcacaca tggccaaacg
     3961 gccagcccga ccaccatggg caaagaactg gcgaacgtgg tttaccgttt gcaacgccag
     4021 ctcaagcaac tgcaaaccaa cgaagtattg ggcaagatca atggtgcagt gggtaacttt
     4081 aatgcgcact tatctgccta tccggatttc gattgggaaa gctttgccaa gcgctttgtg
     4141 gaatcactgg ggctgaccta taacccgatg accatccaga ttgagccaca cgactatatg
     4201 gctgaactgt atgacaccct gtcacgcatc aataccattt tgatcgacct taaccgcgac
     4261 atctggggct atatttctgt cggctacttc aaacagaaag tcaaggcagg tgaaatcggc
     4321 tcctctacca tgccacacaa agtcaacccg attgactttg aaaactcgga aggcaacctc
     4381 ggcctggcca atgccgttct gcgccacctc gccgagaagc ttccgatctc ccgctggcaa
     4441 cgagacctga cggattccac cgttctgcgt aatatgggtg tggcctttgg ttatgccctg
     4501 cttggctacg attcctgcct gcgcggcttg aataaactcg aagttaaccc gcaacgcttg
     4561 ctggaagact tggacaatag ctgggaagtc ctggccgagc ctatccagac ggtgatgcgc
     4621 cgttatggca tcgaaaatcc ttacgagcaa ttgaaggaat tgacccgtgg taaaggcggc
     4681 atcaacaagc aatctttgca tgcctttatt gaaaccctgc aaatccccgc agatgccaaa
     4741 aaagcattgc tggcgctcac accagcaact tataccggca aagcaagtca actggtaaaa
     4801 catatttaaa tgttgccgat ttgatccggg attttcttgc ttaccatgac aaacgtatca
     4861 ctttagagaa agcagcgcgt cacgtgatgc gctgcttaaa gttaaagtgc gtgcgcttaa
     4921 tgcactttaa ccttgcctta tttctgacct ctaccacggc gctggcacag gaagcatcgt
     4981 ggttcgaggt caaacccggc aacacctatc aaacacgctt ctcactggtc acggactcgc
     5041 caccagaaaa
//
LOCUS       DATMSK010000011         4161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_212004,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000011 DATMSK010000000
VERSION     DATMSK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212004"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..737)
                     /locus_tag="VNZ84_00360"
     CDS             complement(12..737)
                     /locus_tag="VNZ84_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWU65428.1"
                     /translation="MTSSKLPSMVQPELLDGDDVTHESWRAFEIMAEFVGATESLKAI
                     MPAVSIFGSARTKPDHPYYLLTEEIARLLSDSGFSVLSGGGPGIMEAANKGAFPGRGA
                     SVGLNIKLPFEQHGNPYQDIGISFKYFFMRKVMFVKYASAFVVMPGGFGTLDELMEAI
                     TLVQTGKSRKIPIILVGKQYWAGLVDWLSNTLLAEGMASPADMDLIQIIDEPQQIVEA
                     IFKHYETTGFELTPAERKMQLYL"
     gene            833..3592
                     /gene="polA"
                     /locus_tag="VNZ84_00365"
     CDS             833..3592
                     /gene="polA"
                     /locus_tag="VNZ84_00365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWU65429.1"
                     /translation="MKTLLLVDGSSYLYRAFHAMPDLRNSANEPVGAIQGVLNMLRRL
                     HKDYPSDYSACVFDAKGKTFRDDLYPEYKANRASMPDDLRAQIEPLYETIRAMGWPLI
                     IEEGVEADDVIGALAKQAEKEGIKTIISTGDKDITQLVNEHITVVNTMRDAFRKTDDV
                     LDIAGVENKFGIPPERIIDYLILVGDTADNVPGVEKVGPKTAVKWLTEYGTLDNIIAN
                     ADNIKGVVGENLRKSLSWLPTARELITIRCDIGIREHFNELIPQAPDKVKLLALFDHF
                     EFRTWKRELDKLGDGVPSTASNTTSETVAGPASQRTEYTPDTRRQYQAIFTEAELESW
                     LSRLQQAELLCFDTETTSLDPMQAKIVGASFSVTPGEAAYIPLTHDYFDAPAQLPLNQ
                     TLEKLKPILENPAIKKVGQNLKYDMHVLANHGIHLQGIAHDTLLESYVFESHKGHGMD
                     ELSDRHLGIEPIPFEAVAGKGAKQVSFNQVTVEQATEYAAEDADITLQLHHALYPQVA
                     ESSKLEFIYQQVEMPSMLVLQRMERNGVLIDSHMLNRQSNEIGLRLMELEKQAFELAG
                     QPFNLGSPKQLQEILFGKLGIKPLKKTPSGVPSTDEDVLQELALDYPLPKVLLEYRGL
                     AKLKSTYTDKLPKMINAQTGRVHTSYNQAVAITGRLASSDPNLQNIPVRTAEGRRIRE
                     AFIAPTGSHIVSADYSQIELRIMAHLSQDAGMLTAFANNEDIHRATAAEIFGVDKSTV
                     DNEQRRYAKVINFGLIYGMSAFGLAQNLNIERSAAQSYIERYFARYPGVRKYMNDTRE
                     LAKQQGYVETIFGRRLWVPEINSPNGNRRAGAERAAINAPMQGTAADLIKLAMIAVDK
                     WLIEEKLQTKLIMQVHDELVLEVPDSELALVQQTLPQLMQSVAKLDVPLLAEVGTGSN
                     WESAH"
     gene            3661..3879
                     /locus_tag="VNZ84_00370"
     CDS             3661..3879
                     /locus_tag="VNZ84_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65430.1"
                     /translation="MKSSLMIGIILIALGAAGLVFKGFSYESKETVAKIGSLEASADI
                     TKDVEIPQALSIIVLAAGVAMVLVGLKK"
BASE COUNT         1133 a         1066 c         1009 g          953 t
ORIGIN      
        1 aaatgagtca tttagaggta aagctgcatt ttgcgttctg ccggggtgag ttcaaagcca
       61 gtcgtttcat agtgtttaaa aatggcttcg acaatctgct gcggttcatc aatgatctga
      121 atcaggtcca tatctgcggg agaagccatg ccctctgcta acagcgtatt gcttaaccag
      181 tccaccaggc ctgcccaata ttgcttgcca accaggataa tcggaatctt gcggctctta
      241 ccggtctgca ccaaggtgat ggcttccatc aactcatcca gcgtaccaaa gccacctggc
      301 atcacgacaa aggcgctggc atatttgaca aacataacct tgcgcataaa aaagtattta
      361 aagctaatgc caatatcctg gtaaggattg ccatgttgct caaacgggag tttgatattc
      421 aaccccacac tggcaccacg accagggaac gcccctttat tggccgcctc cataatgccc
      481 ggaccgcctc cggacaacac actgaatcca gagtctgata acaaacgtgc aatttcttcg
      541 gtgagcaggt aatacggatg gtcgggcttg gtacgtgcac tgccaaaaat cgacaccgcc
      601 ggcataattg ccttaaggct ttcggttgcg ccgacaaact cggccatgat ttcaaacgca
      661 cgccacgatt catgggtgac atcatcgcca tcaagtaact ctggctgcac catgcttggt
      721 agctttgagc tggtcataca acggtccttc atattacaaa ccaaattagt gtacatgaag
      781 tcgctttgaa atgcgtaaaa tacgccattg atttgattca agagaacgca caatgaaaac
      841 cttgcttttg gtagatggct ccagttacct gtatcgcgcc tttcatgcca tgccggattt
      901 acgcaactca gccaatgaac ccgtgggggc gattcaaggc gtactgaaca tgctgcgccg
      961 cctgcataag gactatccat ctgattacag tgcctgcgta ttcgatgcca aaggcaaaac
     1021 attccgcgat gatttgtatc ccgaatacaa agccaatcgc gccagcatgc cggatgattt
     1081 gcgcgcgcag atcgaaccgc tttatgaaac catacgcgcc atgggctggc cgctgattat
     1141 tgaagaaggt gtggaagcag acgatgtgat tggcgctctc gccaaacagg cagaaaaaga
     1201 aggcatcaaa accattattt ctaccggtga caaagacatc acccagctgg tcaatgagca
     1261 tatcactgtc gtgaatacca tgcgcgatgc tttccgcaaa accgatgatg tgctggatat
     1321 cgctggcgtg gaaaacaagt ttggtatccc ccctgaaaga attattgatt acctgattct
     1381 ggtcggtgat accgctgaca acgtgccagg cgtggaaaaa gtcggcccaa aaacagcggt
     1441 aaaatggcta accgaatacg gcacactgga taacattatt gccaacgctg acaatattaa
     1501 aggcgtggtc ggtgaaaacc tacgcaaatc cttgtcctgg ttgccaactg cacgcgagtt
     1561 gatcaccata cgctgtgata ttggtatccg cgaacacttt aatgagctga tcccgcaagc
     1621 gccagacaag gtcaaactac tggctttgtt tgatcatttt gaattccgta cctggaaacg
     1681 cgagctggac aaacttggtg atggcgttcc ctccactgcg tcaaacacaa catcagaaac
     1741 cgttgcgggc ccagccagcc agcgtactga atatacacct gacacacgtc gccagtacca
     1801 ggcaattttc acagaagcgg agctggagag ttggttatct cgactacagc aagcagaact
     1861 gctgtgtttc gatacagaaa ccacttcact cgaccccatg caggcaaaaa tcgttggcgc
     1921 gtcattcagc gtcacgcctg gtgaagcggc ttatattcca ttaacacacg actattttga
     1981 tgcaccagcg cagttgccac tcaatcaaac gctggaaaaa ctgaagccta ttctggaaaa
     2041 tccagccatc aagaaggtcg gccagaatct caaatacgat atgcatgtac tggccaatca
     2101 tggtattcat ctgcaaggca ttgcgcacga cacactgctc gaatcctatg tgttcgagag
     2161 ccataaaggc catggcatgg acgaattgag cgaccgccac ctcggtattg aacctatccc
     2221 gtttgaggca gtggctggca aaggcgccaa acaggtgagc tttaatcagg tgacggttga
     2281 acaagccact gaatatgccg cagaagatgc cgacattacc ttgcaactgc atcatgcgct
     2341 ttatccgcag gtggcagaat cttccaagct ggaatttatc taccaacagg ttgaaatgcc
     2401 cagcatgctg gtgttacagc gcatggaacg caatggcgta ctgattgaca gccatatgct
     2461 gaatcgccaa agcaacgaga tcggcttgcg gctcatggaa ctggagaaac aggcatttga
     2521 actggcaggc caacccttta atcttggcag ccctaaacag ctacaggaaa tcctgttcgg
     2581 caaactgggg atcaagccgc ttaagaaaac accatccggg gtgccgtcta ccgatgaaga
     2641 tgtgttgcag gaattggcgc tggattaccc gttgccaaaa gtgctactgg aatatcgtgg
     2701 cttagccaaa ctgaaatcca cttacaccga caagttgccc aaaatgatta atgcgcaaac
     2761 cgggcgcgta cacaccagct acaaccaggc cgtcgccatt acaggccgcc tggcaagctc
     2821 agaccccaac ttgcaaaaca ttccggtgcg tactgccgaa ggtcgccgta tccgtgaagc
     2881 gtttattgcg ccgactgggt cacacattgt ctcggcggac tattcgcaga ttgaattgcg
     2941 catcatggcg catctatcac aggatgcagg catgctcact gcatttgcca acaacgaaga
     3001 tattcatcgt gccacagcgg ccgaaatctt tggggtggac aaatccacgg tagataacga
     3061 acagcgccgt tatgccaaag tcattaattt tggattgatt tatggcatga gtgcctttgg
     3121 cctggcgcaa aacctcaata tcgaacgcag cgccgcgcaa agctatatcg agcgttactt
     3181 tgcacgctat ccaggcgtac gcaaatatat gaatgacaca cgcgagcttg ccaagcagca
     3241 gggttatgtt gaaaccattt tcggccgccg cctgtgggta cctgaaatca acagcccaaa
     3301 cggcaatcgc cgcgcaggtg ctgagcgcgc cgccattaat gcgcccatgc aaggcacggc
     3361 tgccgacctg attaaactgg ccatgattgc cgtagacaaa tggttaattg aagaaaagct
     3421 gcaaaccaag ctgattatgc aggtacacga tgaactggta ctcgaagtgc ccgactcgga
     3481 actggcactg gtccagcaaa ccttaccgca actcatgcaa agtgttgcca aactcgatgt
     3541 ccccttactg gcagaggtgg gaactggcag caactgggaa agtgcgcatt aaacccatcg
     3601 cttatgctat ggtcatattt caatgattca gtcaccaaaa atcaaccaca ggggaacaca
     3661 atgaaatcat cactcatgat aggcattatt ctgattgcac taggtgcagc cggactggta
     3721 ttcaaaggct ttagctacga gagcaaggaa accgtcgcca aaattggctc gctggaagct
     3781 agcgctgaca tcaccaaaga tgtcgaaatt ccacaggcac tcagtatcat tgtgctggcc
     3841 gctggcgttg ccatggtact cgtaggttta aaaaaataat cctgaaagat ctgcttgcca
     3901 gtcttttctg acaggatttt cctacaatct ctgacagatt cgcttcacag ttctggttag
     3961 cataagcgct catcacaaca acaagggagt acacacatgc tttggacgat tgtcgtaggt
     4021 attatcgcgg gttggttggc aggcttaatt atgaaaggtg atggctacgg cctgatctgg
     4081 gatctgattc tggggattgc tggcgcagtt gttggcggct ggctgtttgg cgcactcggc
     4141 ctgagtgcca gtggcggcat g
//
LOCUS       DATMSK010000012         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_235620,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000012 DATMSK010000000
VERSION     DATMSK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235620"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..542
                     /locus_tag="VNZ84_00375"
     CDS             33..542
                     /locus_tag="VNZ84_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWU65431.1"
                     /translation="MKPVHPIKRWLKHAWSHPRHVKALFPQHSLKQIELVIAQSERHH
                     AGEIRVVIESGLPSYAILRGLTPRQRAINIFGHLNIWDTEHNNGVLIYLLLADHDVEI
                     VADRGIHRHVGDAGWQQICQHMEGMFRSGQFESGVVYGIQAIGEHLAKHFPPEAVPRN
                     ELSNRVVVL"
     gene            649..1155
                     /locus_tag="VNZ84_00380"
     CDS             649..1155
                     /locus_tag="VNZ84_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65432.1"
                     /translation="MQSDTLSLTPAEWDNINRLFTPLPQTPEAERTAIAQAVGVLEDI
                     VGTKIGTAADRAGTFNNSDYLHQQDCNDEAINTTTYMRLMQQAGLLRFHDILDTRTRK
                     FFFTGWPHTAAVIREQASQAEYAVDSWFYDNGYPATIVPMSIWKEGYIPEDSPIVQRP
                     QQKDPPTE"
     gene            1223..2932
                     /locus_tag="VNZ84_00385"
     CDS             1223..2932
                     /locus_tag="VNZ84_00385"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWU65433.1"
                     /translation="MRASQFFIATQKEAPSDAELISHKLMVRAGLIKKLGSGLYSWMP
                     LGLRVLRKVENIVRDEMNKAGALELLMPAVQPKELWEETGRWAVFGPQMLKIQDRHER
                     DFCFGPTHEEVITDIARKEISSYKQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMKDA
                     YSFHTSADCLVNTYNTMHTAYSNVFTRLGLKFRAVKADSGAIGGDGSQEFHVLADSGE
                     DALAYCEQSDYAANVELAEAVATGSRAAATETMREVDTPKQTTCEDVAKLLEVPVSKT
                     VKSVALMVDDVFTLVLLRGDHQLNEVKFGKVEGVKEFRLATEEEIRANLNCPPGFIGP
                     VGVNANVRVVADRTVALMSDFVCGANKPKFHLAGVNFGRDLPEPAIVADIRNVVEGDA
                     SPDGKGMLSLCRGIEVGHIFQLRTKYSEAMQATYLDENGQTQVMEMGCYGIGVSRIVG
                     AAIEQGNDDKGIIFPLSMAPFSVVICPIGLDKSDAVKQAAYDLYAQLQQAGVDVLLDD
                     RGERPGVMFAESDLMGIPHRVVIGDRGLAEGKVEYKGRTDAEAQNLQVTECVTFLRDK
                     LGQ"
     gene            2997..3629
                     /locus_tag="VNZ84_00390"
     CDS             2997..3629
                     /locus_tag="VNZ84_00390"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWU65434.1"
                     /translation="MKKVFFLSFVLVLVGLCAWSAPTMAGNQQEEPLANSVKALMQKS
                     VSDLAAPRLLFDSEQAAQQWLQEMSARLAKRVPDTQYREDFLKSVHYEATRAGLDPQM
                     VLGLIQVESGFKKYAVSSVGARGYMQVMPFWVRAIGTNEHNLFDMRLNLRYGCTILRH
                     YLDIERGDYYRALGRYNGSLGKPEYPNLVVGAWRNHWHYAPSIKMEIASQ"
BASE COUNT          891 a          849 c         1024 g          908 t
ORIGIN      
        1 tctggtgact ggtaaggggc ttttgaaatt atatgaaacc tgtacatcca attaaacgtt
       61 ggctcaagca tgcctggagc catccgagac atgtgaaggc cctgtttccg cagcactcgc
      121 tcaagcagat tgaactggtg attgcgcaaa gtgagcgtca tcacgcgggt gaaatccgtg
      181 tggtgattga gtccggcttg cccagctatg ctattctgcg aggcttgacg ccaaggcaac
      241 gcgcgatcaa catcttcggg catctcaata tctgggatac agaacataat aatggcgtgc
      301 tgatttattt gttgctggct gatcacgatg tggaaattgt tgctgaccgc ggtattcatc
      361 ggcacgttgg cgacgctggc tggcaacaga tttgccagca tatggaaggg atgttcagaa
      421 gtgggcagtt tgagtctggt gtggtgtatg gcatacaagc gattggtgag catttggcaa
      481 agcattttcc accggaggcc gttccacgta acgaattatc taaccgggtg gttgtgcttt
      541 agtttttgtg tagcgctacc tgcccatgcg gccctgaaag atatctaccg tatctaccat
      601 accactccct cactcaatgc ctttgaaatt tgccaaggtg gcggatgtgt gcaatctgat
      661 actcttagcc tcactcctgc tgagtgggat aacattaacc ggttattcac acccttgccg
      721 caaaccccag aggccgagcg aacagccatt gcccaggctg tcggcgtact cgaagacatc
      781 gtgggcacca agataggcac ggcggctgac cgtgccggga cattcaataa tagtgactat
      841 ctgcatcaac aagactgtaa cgatgaagcc atcaatacca caacttatat gcgcctgatg
      901 cagcaggcag ggttgctccg ctttcacgat attctggata cgcgtacaag aaagttcttt
      961 tttactggct ggccgcatac ggccgctgtc atccgcgagc aagcttctca ggcagaatat
     1021 gcagttgata gctggtttta cgataatggc tatccggcaa cgattgtgcc tatgtctatc
     1081 tggaaagagg gctatattcc ggaagacagc cccatcgtgc aacgaccaca gcaaaaagac
     1141 ccgccgacag agtaggcgcg agtgacgccg ccttagaata aggctaaaat cgcgctttta
     1201 gatttcaatt gaatacattt ttatgcgcgc ctcacaattt ttcattgcca cccaaaaaga
     1261 agccccttcc gatgccgaac tgatcagcca caaactgatg gtgcgtgccg ggctgatcaa
     1321 aaagctgggc agtggtttgt atagttggat gccactgggt ttgcgcgtgt tgcgcaaagt
     1381 ggaaaatatc gtgcgcgacg agatgaacaa ggcgggtgca ctggagttgc tgatgcctgc
     1441 cgtacagcca aaagaattgt gggaagagac cggccgctgg gcggtgtttg ggccacaaat
     1501 gctcaagatt caggaccgac atgaacgtga cttctgtttt ggccctacgc atgaagaagt
     1561 gattactgac attgcgcgca aggaaatcag cagctataaa caattgccgc tcaattttta
     1621 ccagatccag accaagttcc gtgacgaaat ccggccgcgt tttggcgtga tgcgcgcacg
     1681 cgaattccta atgaaagatg cctactcttt ccataccagc gcggactgcc tggtgaatac
     1741 ttacaacacc atgcacacgg cttacagcaa tgtgtttacg cgcctgggcc tgaagttccg
     1801 tgcagtgaag gccgactctg gcgccattgg aggtgatggt tcgcaagagt ttcacgtctt
     1861 ggccgatagt ggcgaagacg cattagctta ttgcgagcag tctgattatg cggccaacgt
     1921 ggagctggcc gaagcggttg cgactggcag ccgtgctgcg gcgactgaaa ccatgcgtga
     1981 agtggatacg cccaaacaaa cgacttgtga agatgtcgct aaactgctgg aagtgcctgt
     2041 gagcaaaacg gttaaatctg tcgcattgat ggtagatgac gtgtttacgc tggtattgtt
     2101 gcgtggcgac catcagctca atgaagtgaa atttggcaag gtcgagggcg taaaggagtt
     2161 tcgtctggcg accgaagaag aaattcgagc caacttgaac tgcccacctg gctttattgg
     2221 cccggttggc gtcaacgcca atgtgcgtgt agtggctgac agaaccgtgg cgctcatgag
     2281 cgactttgtc tgtggcgcca ataaacctaa atttcacttg gcgggcgtca attttggccg
     2341 tgatttacct gaaccagcca tcgtggcaga tatccgcaat gttgtcgaag gcgatgccag
     2401 cccggatggc aaaggcatgt tgagtttatg ccgtggcatt gaagtgggcc acatttttca
     2461 attgcgtacc aagtattccg aagccatgca ggcgacttac ctggacgaaa atggtcagac
     2521 gcaagtgatg gaaatgggct gttatggcat cggcgtatca cgcattgtag gcgcagcgat
     2581 tgagcaaggt aacgatgaca agggcattat tttcccgctg agtatggcac cattctcagt
     2641 cgtgatttgc ccgattggcc tggacaagag cgacgcagtc aaacaagctg catatgacct
     2701 ctatgcccag ttgcaacaag ccggtgtgga tgtgctgctg gatgaccgtg gtgagcgccc
     2761 aggtgtcatg tttgccgaaa gtgacctcat gggcatccca catcgcgtgg tgattgggga
     2821 tcgtggtttg gcagagggta aagtcgaata caaaggtcgt accgacgctg aggcacaaaa
     2881 cttgcaagtg actgaatgtg tgaccttttt gcgtgacaaa cttggtcagt aattagaggg
     2941 agatcatccc tctgttttgc gcttttgatg cgctcattgt gcttaaaatg gacgggatga
     3001 aaaaagtatt ttttctttct tttgtgctgg tgttagtggg cctgtgtgca tggtctgctc
     3061 ctaccatggc tggcaaccag caagaggaac cgctagccaa tagcgtcaag gcgctgatgc
     3121 aaaagtctgt cagcgacttg gcggcaccta gattgttatt tgactctgag caagcagcac
     3181 agcagtggtt gcaagagatg tctgcccggt tggccaagcg ggtccctgat acgcaatatc
     3241 gcgaagactt cctgaagtcc gttcattatg aagcaacacg tgctgggtta gacccacaaa
     3301 tggtgctagg tttgattcag gtggaaagtg ggtttaaaaa atatgcagtt tccagcgtgg
     3361 gtgcccgtgg ttatatgcag gtgatgccat tctgggtgag agctataggt accaatgagc
     3421 ataacttgtt tgatatgcgt ttaaacctgc gctacggctg cacgatttta cgccattatc
     3481 tggatattga acgtggcgat tactatcggg cacttgggcg ttataacggc agtctgggta
     3541 agccggaata tcctaacctg gtggtggggg cctggcgtaa ccattggcat tacgctcctt
     3601 ccatcaagat ggagattgct agccagtagc cgtaagggtg acaataaaaa accgcgccat
     3661 ggcgcggttt tt
//
LOCUS       DATMSK010000013        10161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_282706,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000013 DATMSK010000000
VERSION     DATMSK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282706"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..845
                     /gene="rpiA"
                     /locus_tag="VNZ84_00395"
     CDS             171..845
                     /gene="rpiA"
                     /locus_tag="VNZ84_00395"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HWU65435.1"
                     /translation="MNDKQAVALEAASYVKNDMIVGLGTGSTANYFIEALAERQKTEG
                     LRVTTVASSNISMIKAQSVGLTVISLAQISQIDLYVDGADEITPDNTLLKGRGYDLVM
                     EKLLAKAAKEFIVVADKSKLVDRIGTHYPIPIEVMPFAWQAAKRSIEAIGGVGDLRPN
                     TAKDGLCISSYGSLVLDMRFDASLDAHTLNALLNNVPGVVEHGIFAKLAKTILIADQG
                     KVETRV"
     gene            858..2330
                     /gene="mltF"
                     /locus_tag="VNZ84_00400"
     CDS             858..2330
                     /gene="mltF"
                     /locus_tag="VNZ84_00400"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HWU65436.1"
                     /translation="MWGYSSKSVRQVWLKVMLATTLCAALLSCSDNNAPKQLVHAKPK
                     PDPYAIRFVTINSPSTYFIDSQNNYAGLEYDLAKRFVEFLGPPYSLKFIVVDSFDKVF
                     PTLLRGDADIAAADISITAARKRQVLFGPAFNQVQSMVVFNRDNNKEPTKAEALLQKR
                     IVLPHSTSFVERMRELQSKLPALTWRETDKLSSEQLIEAVARNEIDFTVADNHLLAIM
                     QYNYPQLDAGMMLGQTDKIAWALNKLNGEERLKQVEAFFKKIEKDGTLRNLLDRYHGY
                     TKRLKPVDITAFLSKSQTLLPKYVRLFKNAQEITDIDWRLLAALSYQESHWDNYSTSP
                     TNVRGLMMLTEQTANMMNVTDRLDPKQSVVAGAKFFLWMKDRIPTRIPEPDRTFMTLA
                     AYNNGVGHLEDARIIAQRLGLNPDSWADVKRGYQLLNDPAYYVNARHGYCSCGAPVIY
                     TELVRSYYQIMQKYFPAYDPGVDPYKIADIKMPWLLAKAS"
     gene            complement(2349..3623)
                     /gene="serS"
                     /locus_tag="VNZ84_00405"
     CDS             complement(2349..3623)
                     /gene="serS"
                     /locus_tag="VNZ84_00405"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWU65437.1"
                     /translation="MLDIQQLRNDLDTVVARLKARKFEFPADEFKALEAQRKTIQTNT
                     ENLQAKRNAASKQIGIAKSKGEDASAILAEVAGLGDELKAAEAQLNEIQASMQALMLN
                     LPNLPHESVPVGQDETQNLEVRRWGSPRSFDFEVKDHADVGAPLGLDFDTGAKLSGAR
                     FTLMKGQIAKLHRAIAQFMLDTQTDKHGYTECYTPYMVNRETLVGTGQLPKFEEDLFA
                     TPHNDSKLYLIPTSEVTLTNTVRDEIVALDTLPIKLTAHTPCFRSEAGSYGRDTKGMI
                     RQHQFDKVEMVQIVHPETSYQALEDMVGHAEAILQALELPYRVVSLCTGDMGFGAAKT
                     YDLEVWLPAQNTYREISSVSNCEAFQARRLQARFRNANGKPELLHTLNGSGLAVGRTL
                     VAVLENNQNADGSVTIPKALQPYMGGKTVITA"
     gene            complement(3623..4948)
                     /locus_tag="VNZ84_00410"
     CDS             complement(3623..4948)
                     /locus_tag="VNZ84_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWU65438.1"
                     /translation="MSSLFEPQTPSSVPLAEQLRPKTLDEVVGQTHLLGEGKPLRRAF
                     ESGKLPSMILWGPPGVGKTTLARLIAQTADAEFVPLSAVLSGIKDIREAVERAELTLQ
                     QTGRNTILFVDEVHRFNKGQQDAFLPFVESGLITFIGATTENPSFEVNSALLSRAQVF
                     VLNALTEAELAELLERARLKVASTVSLTDEVKEQVLAYADGDARRLLNFAEGLFNGAV
                     AAKVTEIDESFLQSTMASKMRRFDKGGEAFYDQISALHKSVRGSNPDAALYWLMRMLD
                     GGADPLYLARRIIRMAWEDIGLADPRAMQIANDAALTFERLGSPEGELALAQAVIYLA
                     VAAKSNAGYMAYNQAKAFVAQDKSRMVPLHLRNAPTKLMKALDYGKEYRYAHNEPGAY
                     AAGEQYFPDDLQPQQFYQPTPRGLEGKIGEKLNHLKELDQQYLHSKAKK"
     gene            complement(4968..5591)
                     /gene="lolA"
                     /locus_tag="VNZ84_00415"
     CDS             complement(4968..5591)
                     /gene="lolA"
                     /locus_tag="VNZ84_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HWU65439.1"
                     /translation="MSKVLNKVLLAVCSLCVISTAFADGVDDLKQFYQSTQAMRANFK
                     QTVFDQKGRKLQEVNGTMLLQRPNKFRWDYQKPYEQQIISDGKQVFLFDVDLQQVTVR
                     TVDKVLGTSPAALLAGGPNVEETFRLTRLLDFEGMQRVQAKPKQKDSGFQVVIISFDH
                     SKLAEMRLVDAFGQSTHIVFSNVEVNPSLSTNQFLFAIPKGVDVVGE"
     gene            complement(5584..7896)
                     /locus_tag="VNZ84_00420"
     CDS             complement(5584..7896)
                     /locus_tag="VNZ84_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWU65440.1"
                     /translation="MFFNKKKVANVRLEKVSTEKQLRDAKLVREVAWIALAAFGVFLA
                     VILATYSHEDPGWTHAVGESDTIHNAGGAVGAWVTDILLSLFGFSSWWFVVLAFFFVW
                     LMYQRLHLETGEKNEWWLNGVGFALMLLASAALESGHLVSFPADFPLKQGGVIGSGID
                     HLLRNMFGFAGSTMLLLVLFATAFSLFTGWSLISMSEKLGEWVIQFYQWSLLKYQDSQ
                     DRKVGRQALQAREEFVEAERKRIEDRKPVEITVPEVVIEPSERVIKEKQAVLFETIAQ
                     EGLPALHLLDAPNQSVELQSAETLDFTSRLIERKLMDFGIEVKVVVAQPGPVITRYEL
                     DPAPGVKGSQITNLAKDLARALSVISVRVVETIPGKSYMGLEIPNPKRQIVSLSEIMG
                     SQAYADLNSPLAIAMGKDIAGKPIVADLAKMPHVLVAGTTGSGKSVAINALILSLLYK
                     SEASRVRMILIDPKMLELSVYEGIPHLLAPVVTDMRQAANALNWCVAEMERRYKLMST
                     LGVRNLAGYNQKIQDAKKQGQSIPHPFSLTPDEPEPLDEMPLIVVVIDELADLMMVVG
                     KKVEELIARLAQKARASGIHLVLATQRPSVDVITGLIKANVPTRVAFQVSSKIDSRTI
                     LDQMGAETLLGQGDMLYLPPGSGYPQRIHGAFVSDGEVHNVVNFLKAQGEPNYIEGIL
                     TNETEGGDLDGVMGGDDAGGEKDPLYDEAVAIVLKTRRASISSVQRQLRIGYNRAARL
                     IEDMERAGLVSAMQSNGNREVLVKAGGDHE"
     gene            8007..8468
                     /locus_tag="VNZ84_00425"
     CDS             8007..8468
                     /locus_tag="VNZ84_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HWU65441.1"
                     /translation="MDIGINNEDRQQIAEGLSKLLADTYTLYLKTHYFHWNVTGPMFN
                     TLHLMFETQYTELALAVDLVAERIRSLGVYAPGTYAQFSKLTSIDETVDVPKANDMIR
                     ELVAGHEAVCRTARSVFPAAEKASDEATSDLLTQRLQLHEKTAWMLRSLLE"
     gene            8561..9511
                     /gene="trxB"
                     /locus_tag="VNZ84_00430"
     CDS             8561..9511
                     /gene="trxB"
                     /locus_tag="VNZ84_00430"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWU65442.1"
                     /translation="MATKHARLMILGSGPAGYTAAVYAARANLNPVLITGLAQGGQLM
                     TTTEVDNWPADANGVMGPELMARFQQHAERFNTEMIFDHIHTTHLKEKPIRLVGDNGE
                     YTCDALIIATGASAKYLGLPSEEAFAGKGVSACATCDGFFYRNQEVAVIGGGNTAVEE
                     ALYLANIASKVTLVHRRDKFKAEAILVDKLMDRVKEGKIVLETFHTLDEVLGDNTGVT
                     GMRVKDVNSGNTKDIALKGVFIAIGHQPNTQIFEGQLEMEGGYIVTQTGRQGNFTATN
                     IPGVFAAGDVQDHIYRQAVTSAGTGCMAALDAERYLTSLE"
     gene            9576..10121
                     /locus_tag="VNZ84_00435"
     CDS             9576..10121
                     /locus_tag="VNZ84_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HWU65443.1"
                     /translation="MPQEHPDQDENDISLLHEAVKGARPVKTNRISHAPKKPKPIPRQ
                     LIRDEQQALVDSLSDDYIPAHELESGEELLYLREGQSPMVLSKLRRGHWVVQAHIDLH
                     GLISDEARLYVSEFIADCKKRGLRCVRIVHGKGLGSRNKEPVLKHKLRNWLMQKDEVI
                     AYAQARANDGGSGAVIVLLKA"
BASE COUNT         2599 a         2668 c         2480 g         2414 t
ORIGIN      
        1 gtatttctag aaatgcacat tgcgatcccc actttacgag tgaggccagg caaggcgcga
       61 ttaagaattt ttgataccag aataggtcat aggttgaaag gatgcttatg cgcgccatgg
      121 caaacaaaag tattgaggga aatgccccaa accagcacgg agaataaacg atgaatgaca
      181 aacaagccgt tgcgctggaa gctgccagct acgtcaagaa cgatatgatt gtaggattgg
      241 gcacaggatc tacagccaat tattttattg aagcccttgc agagcgccaa aaaactgaag
      301 gcctgcgggt aaccaccgtc gccagctcca atatcagcat gatcaaagcg caaagcgtcg
      361 gcctgaccgt gatcagcctg gctcaaatca gccaaataga tctttatgtg gatggcgccg
      421 acgaaatcac acctgacaat accttgctca agggacgtgg ctatgacctg gtgatggaga
      481 aattactggc taaagccgcc aaagaattta ttgtggtcgc ggacaagagc aagctggtag
      541 accgtatcgg cacgcattac ccgattccga tcgaagtgat gccatttgcc tggcaagcag
      601 ccaaacgtag cattgaagcg atcggtggcg taggcgatct gcgccccaat acggccaaag
      661 atggcttatg tatcagcagc tatggcagct tggtactgga tatgcgtttt gatgccagcc
      721 tggatgccca taccttgaat gccttgctca ataacgttcc tggtgttgtc gaacatggca
      781 ttttcgctaa actggccaag acgatactga ttgcagatca aggcaaggtc gagacacgcg
      841 tttaagcgct agcgcgaatg tggggctatt caagcaagtc tgtgcggcag gtatggctca
      901 aagtcatgct ggccaccaca ctatgtgctg cgctgctgtc atgtagtgac aacaatgcac
      961 ccaaacaact ggtacatgca aagcctaaac cagaccctta tgccatccgc ttcgtcacca
     1021 tcaatagccc cagcacttat ttcatcgata gccagaacaa ttacgcaggg cttgaatacg
     1081 accttgcgaa acggtttgtc gagtttttag gcccgcccta ttcccttaaa tttatcgttg
     1141 tcgatagttt tgacaaggta tttccaaccc tgttgagagg ggacgccgat attgctgcag
     1201 cagatatcag cattaccgct gcgcgtaaac gccaggtact atttggccct gcattcaacc
     1261 aggtgcaatc catggtggta tttaaccggg acaacaataa agagccgacc aaggccgagg
     1321 cgttattaca aaaacgtatc gttctccctc atagcaccag ttttgtggag cgtatgcgtg
     1381 agctgcaatc caaacttccc gcattgacct ggagggaaac cgacaaactc agttcggaac
     1441 aactgatcga agcggtcgcg cgtaatgaga tcgactttac cgtcgcggat aatcacctgc
     1501 tggccatcat gcaatacaac tatccacaac tggatgccgg catgatgctg gggcagacgg
     1561 acaaaattgc atgggcgctc aataaattaa acggggaaga gcggcttaaa caggtggaag
     1621 cctttttcaa gaaaattgag aaagacggta ccttgcgtaa cttgctggac cgctaccatg
     1681 gttataccaa acgcttgaaa cctgtagata tcactgcatt tttaagcaaa tctcaaacac
     1741 ttttgcccaa atatgtgcgc ctgttcaaaa atgcacagga aatcaccgac atagactggc
     1801 gcttgctggc tgcccttagt tatcaggaat cacactggga taactacagc acctccccaa
     1861 ccaatgtgcg cggcctcatg atgctcaccg aacaaaccgc caacatgatg aatgtcactg
     1921 accgcctgga tcccaaacaa agcgtcgttg ccggtgccaa attcttccta tggatgaaag
     1981 accgcatccc aacacgcatt cccgagccag accgcacttt tatgacttta gctgcctaca
     2041 ataatggcgt tggtcatctt gaagatgcgc gcattatcgc gcaacggtta gggctgaatc
     2101 cagacagctg ggccgatgtg aaacgtggtt accagttact taacgaccca gcctactatg
     2161 tgaatgccag acatggttat tgcagctgtg gcgcacccgt gatttatacc gagctggtca
     2221 ggagctacta ccagatcatg caaaaatact tcccggctta tgacccaggc gtagaccctt
     2281 acaagattgc agacataaaa atgccctggt tgttagccaa ggcatcttga acacaactag
     2341 cagacgaatc aagcggtaat gactgtttta ccgcccatgt aaggttgcag cgctttagga
     2401 atggtcacac tgccatccgc attctgatta ttttccagca cggccaccag cgtacggcct
     2461 acggccaggc cagaaccatt tagggtatgc aataactctg gcttaccatt agcattgcgg
     2521 aaacgtgctt gcaaacgacg cgcctggaaa gcttcgcagt tagaaacgga agagatttca
     2581 cggtaagtgt tctgcgcggg caaccagact tcgaggtcat aggttttggc agcgccaaag
     2641 cccatatcgc cagtacataa cgagaccaca cggtatggca actccagcgc ttgcagaatg
     2701 gcttcggcat ggccgaccat gtcttccaat gcctggtaac tagtctcagg atgtacaatc
     2761 tgcaccattt caactttatc aaactggtgc tggcggatca tgcccttggt atcgcgacca
     2821 taagagcccg cttctgaacg gaaacaaggc gtgtgggcag tcagtttaat cggcaaggtg
     2881 tccaacgcca cgatttcatc acgcacggta ttggtcagcg tgacttcaga agttggaatc
     2941 agatagagct tgctgtcatt gtgtggtgtg gcgaacaagt cttcttcaaa ctttggcaat
     3001 tggccggtgc caaccaaggt ttcacggtta accatatacg gcgtatagca ctcagtataa
     3061 ccatgcttgt cggtttgcgt atccagcata aactgggcaa tcgcacggtg cagtttggca
     3121 atctggcctt tcatcaaggt aaagcgtgcg cctgacaatt tggcacccgt atcaaaatcc
     3181 aggcccaatg gcgcacccac atcggcatgg tctttaacct caaaatcaaa gctgcgcgga
     3241 gatccccagc ggcgtacctc taagttttgt gtttcatcct ggccgactgg cacgctttca
     3301 tgtggcaagt taggcagatt caacatcagc gcctgcatac tggcctggat ttcattcaac
     3361 tgtgcttctg cggcttttag ctcatcccct aaaccagcca cttctgccaa aatggcactg
     3421 gcgtcttcgc ctttggattt ggcaatgcca atctgcttgg aagcggcatt acgcttggct
     3481 tgcagattct cggtattggt ctggatggtt ttgcgttgcg cttccagagc tttgaactca
     3541 tccgccggaa actcgaactt gcgtgccttc aaacgcgcga ctaccgtatc cagatcattt
     3601 ctaagttgtt gaatatctaa cattattttt tcgctttgct atgtaagtat tgctggtcta
     3661 actctttaag gtgattcagc ttttcgccta ttttaccttc caatccacgt ggtgtgggct
     3721 ggtaaaactg ttgcggttgc aaatcatccg ggaagtattg ttcacccgcg gcataggcgc
     3781 caggctcatt atgggcataa cggtattcct taccgtaatc gagcgctttc atgagtttgg
     3841 ttggcgcatt tcttaaatgc aaaggcacca tacgggattt atcctgtgcg acaaacgctt
     3901 tggcctggtt ataagccata tacccggcat tactcttggc tgctaccgcc aggtaaatca
     3961 cggcttgtgc cagtgccagc tcgccttcag gactgcccaa acgctcgaaa gtcaacgctg
     4021 catcattggc aatctgcatg gcacgcggat cagccaggcc gatgtcttcc caggccatgc
     4081 ggataattct gcgggcaaga tacaatgggt ctgcgccgcc atccagcatg cgcatgagcc
     4141 agtataacgc agcatccgga ttggatccgc gcaccgattt atgcaaggca gaaatctggt
     4201 cataaaacgc ctcaccacct ttatcaaagc ggcgcatctt gcttgccatg gttgactgca
     4261 aaaaggactc atcaatctca gtcaccttgg cggccactgc cccattgaac aagccttcag
     4321 caaagttaag caagcgtctg gcatcgccat cggcataggc taatacctgc tctttcacct
     4381 catctgtcag cgacacggta gaggccactt tcaagcgtgc acgctccaat aactcggcca
     4441 actctgcttc agtcaaggcg ttgagtacaa agacctgtgc acggcttaat aacgcgctat
     4501 tcacctcaaa tgacggattt tcagtcgtcg cgccaataaa ggtgatcaag ccactttcga
     4561 caaatggcaa aaaggcatcc tgctggcctt tgttaaagcg gtgcacttca tcgacaaaca
     4621 atatggtatt gcggccagtt tgctgcaagg taagttctgc acgttcgacg gcttcacgga
     4681 tatctttaat gccggacaac accgctgaca atggtacaaa ttcagcatca gccgtttgtg
     4741 caatcaggcg cgcaagtgtg gttttgccga cccctggcgg cccccacaaa atcatcgaag
     4801 gcaatttgcc cgactcaaat gcacggcgca gaggcttacc ttcaccaagc aaatgcgttt
     4861 gcccgaccac ctcatcaagc gttttcgggc ggagctgctc tgctagcggc acagaggaag
     4921 gtgtttgtgg ctcaaacagg gaggacatga cgaatgatgc gtagcgatta ttcgcctacc
     4981 acatccaccc ctttgggaat ggcgaacagg aactggttgg tgctcaggct aggattcact
     5041 tctacattgg aaaatacaat atgggtgctc tggccaaacg catccaccag gcgcatttcg
     5101 gccagcttgc tatggtcaaa tgagataatg acgacctgga aaccactatc cttttgcttg
     5161 ggcttggctt gcacgcgctg catgccttca aagtccagca gtcgtgtgag acggaaggtt
     5221 tcttcaacat tcggcccccc tgccagcaag gcggctgggc tggtgcctag cactttgtct
     5281 acggtgcgta cggtcacttg ctgtaaatcg acatcaaaca ggaatacctg cttgccatcg
     5341 ctgatgattt gctgctcata aggtttttga taatcccagc gaaatttatt cgggcgctgc
     5401 aacagcatgg tgccattgac ctcttgcagc ttgcgccctt tctggtcaaa cacggtttgc
     5461 ttgaagttgg cacgcatggc ctgtgtgctt tggtagaact gttttaaatc atccacgcca
     5521 tcggcaaatg ccgttgaaat gacacacaga ctgcatactg cgagcagcac tttattcaat
     5581 actttactca tggtctcctc cggctttcac taatacctca cggttgccat tactttgcat
     5641 ggcagagacc agtccggcac gttccatatc ttcaattaat cgggctgcgc ggttgtaacc
     5701 aatgcgcaat tgacgctgta ccgatgaaat cgaggcccgg cgtgttttca acacaatggc
     5761 tactgcttcg tcatagagtg gatctttctc accaccagca tcgtcgccac ccattacacc
     5821 atccaggtcg ccgccttcgg tctcattggt gagaatgcct tcaatatagt ttggttcgcc
     5881 ctgcgccttg aggaagttca ccacattatg cacttcgcca tcgctgacaa aggcaccatg
     5941 gatacgttgt gggtaaccgc tgccaggtgg caagtagagc atatcgccct gccccagcaa
     6001 ggtttcggcc cccatctgat ccaggatagt acgcgagtca atcttgctcg atacctggaa
     6061 ggccacacgg gtaggcacgt tggctttaat caggccggta atcacatcta cagaaggccg
     6121 ttgtgtcgcc agtaccaggt ggatacctga ggcacgcgct ttttgtgcca gacgggcaat
     6181 caactcctcc accttcttac cgacgaccat catgaggtca gccaattcat cgatcacaac
     6241 gacgatcaat ggcatttcgt ccagtggttc cggttcatcc ggtgtcaggc tgaatggatg
     6301 cggaatggat tggccttgct ttttcgcgtc ctgaattttc tggttatagc ctgccaggtt
     6361 acgtacaccc aaagtagaca tgagcttgta acggcgttcc atctcggcca cacaccagtt
     6421 caatgcattc gccgcctgac gcatatcggt cactacaggg gccagcagat gcggaatgcc
     6481 ttcatagacg gacaactcca gcatttttgg gtcaatcagg atcatgcgta cacgactggc
     6541 ttcggattta tacagcaagc tcagaatcaa ggcgttaatc gcgactgatt taccggaacc
     6601 agtggtaccc gccaccagta cgtgcggcat ttttgccagg tcagccacaa tcggcttacc
     6661 ggcaatgtct ttccccatgg caatcgccag cggggagttc aagtcggcat aggcctggct
     6721 acccataatt tcagacaagg atacgatttg acgtttagga ttggggatct ccaggcccat
     6781 gtagcttttg ccgggaatgg tttcgaccac gcgcacactg atcacggaca aggcacgtgc
     6841 caaatctttg gccaggttgg tgatctggct gcctttgacg ccgggtgccg gatccagctc
     6901 ataacgcgta atgaccggac ctggctgcgc cacgaccact ttaacctcaa tgccgaaatc
     6961 catcagtttg cgctcgatca ggcgcgaggt gaagtccaag gtctctgccg attgcaactc
     7021 tacagactgg ttaggcgcat ccagcagatg cagggccggc aacccttcct gagcgatagt
     7081 ctcgaacagt accgcctgtt tctccttgat cacgcgttca cttggctcta tcaccacttc
     7141 gggcacggtg atctctaccg gcttgcggtc ttcaatacgt ttacgctcag cttctacaaa
     7201 ctcttcacgt gcctgcaagg cctgacgccc cactttgcgg tcctggctat cctggtactt
     7261 gagcaagctc cactggtaaa actggatcac ccactcgccc agtttctcac tcatggaaat
     7321 caaagaccag cccgtaaaca ggctgaaggc cgtagcaaac aataccaata acagcatggt
     7381 tgagccggca aagccaaaca tattacgcag caaatgatct atgccactac caatgacacc
     7441 gccctgcttg agcggaaagt cagccgggaa actgaccaga tgcccagact ccagcgcagc
     7501 agaggccagc aacatgagcg caaagccgac gccgttcaac caccattcgt ttttttcgcc
     7561 agtttccagg tgcaaacgct ggtacatcaa ccagacaaag aaaaaagcca gcaccacaaa
     7621 ccaccaggag gaaaaaccaa acagtgatag caggatatcc gttacccatg cgccaacggc
     7681 tcccccagca ttatggatgg tgtcactttc accaactgca tgggtccagc caggatcttc
     7741 atgtgaataa gtggccagaa tcacagccag aaacacgccg aacgcggcca gggcaatcca
     7801 tgcgacttca cgcaccagct tggcatcccg cagctgcttt tcggtcgaaa ctttttcaag
     7861 ccggacgtta gcaacttttt tcttattaaa aaacaatatg ttgtctcttt ctttgcaaga
     7921 gtgaacttaa atgattataa caaaatcaaa ttgcagactg tcattctagc ttttaaaata
     7981 cattccacga caataaggag acatccatgg acattggtat caacaacgaa gaccgtcagc
     8041 agattgcaga gggcttatca aaattactgg cagatacgta cactctgtat ctgaaaacac
     8101 attactttca ctggaatgtg accgggccga tgttcaacac cttgcacctg atgtttgaaa
     8161 cacagtacac cgagctggca ttagcggtag acctggtggc tgaacgtatc cgctcattgg
     8221 gcgtatatgc acctgggacc tatgcgcagt tctccaaact gacgtcgatt gatgaaacgg
     8281 tcgatgtgcc caaggcgaat gacatgatcc gcgagctggt tgctggtcat gaagccgttt
     8341 gccgtaccgc gcgcagtgta ttcccagccg ctgaaaaagc ctcagatgaa gccacttctg
     8401 atttactgac acaacgtctg cagttgcacg agaaaacggc atggatgctg cgcagcttgt
     8461 tggaataatg ctctagcact tttcttcagg atgggcattt aaatatagat tgcccatccc
     8521 catctatata aaaataattc accatattgg aaatctcatc atggcaacaa aacatgcccg
     8581 tttaatgatt cttggctctg gtcctgcggg ttacactgca gcggtctacg cagcgcgagc
     8641 taaccttaat ccggtgttaa ttaccggcct ggctcagggc ggccagctga tgaccaccac
     8701 cgaagtcgat aactggccag ccgatgccaa tggtgtgatg gggccagagc tcatggcacg
     8761 ctttcagcaa catgctgagc gctttaacac cgagatgatt tttgatcata tccacaccac
     8821 acatctgaaa gaaaaaccga tccgcctggt gggagataac ggtgaatata cgtgcgatgc
     8881 cttgatcatt gctaccggcg cgtcggccaa atatctgggc ttacctagtg aggaagcctt
     8941 tgcaggtaaa ggcgtctcgg cctgtgccac ctgtgacggt tttttctacc gcaaccagga
     9001 agtcgctgtc attggcggcg gcaatacggc tgtggaagaa gcgctgtacc tggccaacat
     9061 tgccagcaag gtcaccctgg tacaccgccg cgacaaattc aaggcggaag ccatcctggt
     9121 tgataaattg atggaccgtg tgaaagaagg caaaattgtt ctggaaacgt tccatacgct
     9181 ggatgaagta ttgggtgaca ataccggtgt gactggcatg cgcgtgaaag atgttaattc
     9241 cggcaacacc aaggatattg cgcttaaagg cgtatttatt gcgattggtc accagccgaa
     9301 tacccaaatt tttgaaggcc agctggaaat ggaaggcggc tatatcgtga cgcaaaccgg
     9361 tcgccagggt aacttcaccg ccaccaatat tcctggtgtg tttgctgccg gcgacgtaca
     9421 agaccatatt taccgccagg ccgtgactag tgcaggtaca ggttgcatgg ctgcattgga
     9481 tgcggagcgc tatttgacca gcctggaata aatccacgta gctaagaaat gaagaagcgc
     9541 accccggtgc gcttttttta ttttagaatg aatatatgcc acaagaacac ccggaccaag
     9601 acgaaaatga tatttcactg ttacatgaag cagtgaaagg tgcgcgtccg gtaaaaacca
     9661 atcgcatttc acatgcgcca aaaaagccga aacccattcc aaggcagttg atccgagatg
     9721 agcaacaagc cttagttgat tcacttagtg acgactacat cccggcccac gaactcgaaa
     9781 gcggcgaaga gttgctgtat ttgcgtgaag gccagtcgcc catggtatta agcaagttac
     9841 ggcgcggaca ctgggtcgtg caagcgcata tcgatttgca tggtctgatc agcgacgagg
     9901 caagattgta tgtgtctgaa tttattgctg attgcaaaaa gcgcggcttg cgctgtgtac
     9961 ggattgtgca cggcaaaggc ttgggttcgc gcaataaaga acctgtactc aagcacaaat
    10021 tgcgtaactg gctgatgcaa aaggatgaag tgattgccta tgcccaggcc cgtgccaatg
    10081 atggcggtag tggcgcagtg atcgtgcttc tgaaagcatg aataaaaacg cgatgcagga
    10141 gactgcatcg cgttcgccgt t
//
LOCUS       DATMSK010000014         8321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_399920,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000014 DATMSK010000000
VERSION     DATMSK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399920"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VNZ84_00440"
     CDS             <1..1468
                     /locus_tag="VNZ84_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWU65444.1"
                     /translation="PQVSGMQMTIADIRQRALQNNLQLKVAKMDPELAKQSLREEQAK
                     FDQVIFAYAKYAEKDTPAMSGDKVVIKSDNPLLDGELAKLNLDAQKKEFWDIEAGVKV
                     PLRTGGTVTLSAPLENRQSLGKFASDQYRSALRFSFSQPLLRNAGQQVNEASIRVAEL
                     DRDSVQLKTRLQAIRIVATVDKAYWELYEAWAALDVRKNQFEYASQNLEMVKRRVQEG
                     LTAAVEVNRAQIGVADRMEALIVAETNLKLAQRQLQFLLNDLPDDQERQSPWVPTTVP
                     SLVKFEFDRAHLIRDALDSRIELLEQELKLSADLIQIDYLQNQTLPLFTLDYQYGALS
                     GSGGRFGDIGQDLFDGRYRDWSVGLKFEMPVSNEARKAKLDRAIAQRMQRLTTKTLQS
                     LTVKREIHDALDKVEQNWQRIIAARQQVLVAGINYEAELKQFNEGLRTMTEVLETLTR
                     LGEAQLKEIRAISDYQVSLVDMAYATGTVLGHGQISLQ"
     gene            complement(1472..2113)
                     /locus_tag="VNZ84_00445"
     CDS             complement(1472..2113)
                     /locus_tag="VNZ84_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65445.1"
                     /translation="MFDDFPTDLRQEIAQSAARLMFEEGISDYGRAKRKAVKLLGIHQ
                     DAPLPTNGEIDSALREYHALYADETDAAHLLDLRHAALHTMQLLKKFNPHLIGSVLEG
                     TAGPYADTDIHVFAESAKELEIYLLNQNVPYRLDEQQFRMSDRPGKQKHDMVRKRVPV
                     LLVETRFGTQRISVFEADDIRTAPRKNGEKSGIQRMNLQQLSDVLFPGKVVNE"
     gene            complement(2139..4565)
                     /locus_tag="VNZ84_00450"
     CDS             complement(2139..4565)
                     /locus_tag="VNZ84_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWU65446.1"
                     /translation="MNSRNWLQSLLFLSITGSLIAAALLVLVISILYPSLPSLDALTD
                     YHPKLPLRIYDAQGGLIAEFGQERRAFVPIEQTPKVMKDAILAIEDRRFYQHNGIDTT
                     GILRAIRNNLTGRGHEGASTITMQVAKNFFSGPDAPRNIWTKIKEAMLAIKIENAISK
                     DKILELYLNQIYLGERAYGFATASKTYFDKDLKQLNLAEAALLAGLPKAPSKYDPYIH
                     PDYAIRRQHEVLRDMHRYGLISTQELEQAMNTPLKFQSSRRQIQVLGSEYVTEIVRAS
                     LYKRYGEKAYTSGLRVYTTINNKHQAAANNAVIQGIINFELRHGFRGAEQIISLPAGK
                     LKASEAAELLRDFVTYNQFVPALVTKVEPNLLTLVTKRGTVLTLKDKSLLLVKNHMPP
                     AKRTPLSLVPGAVIRVYQQNDNWLVVQLPEVESGFMALNPETGQVQALIGGFSFQRNK
                     YNHVTQGRRQPGSSFKPFIYSAALEKGYSPASVFEDAPISFTSTETGSNSSWEPRNYD
                     EKYAGPMRLREALAQSKNTISIRLMNAIGARYAQDYIRKFGFAPKDHPAYLSTALGAG
                     STSVWQMAAAYATFANSGYQVHPYLIEKIIDSRGKVIQAVQAGKHVSQARVIDPRNAF
                     IITSMLQDVVRDGTARAALSLRRPDIAGKTGTTNDQFDAWFAGYHPKEVAVAWVGYDN
                     PHNLGRGETGGRAALPIWISYMQQTLPGLPVYQYKVPDGIVQLKVDAYTGAQVGEDDP
                     GIYEYFYEEHLPQSTPENLPGLFDPEGEQPPEDNEPSFLERLIGEHSGGSNDDRKLPS
                     NPAAKLLSPN"
     gene            4776..5849
                     /locus_tag="VNZ84_00455"
     CDS             4776..5849
                     /locus_tag="VNZ84_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HWU65447.1"
                     /translation="MSLGFLQRKKTSFIGVDVSATAVKMVELSAVGKQQFRLDGYANV
                     TLPKGVISDGNINDLTQVAETLRSAWRLLGTRARHAVLALPTAAVISKRVIMPAGMSE
                     EDLELQVESEANQYIPFPLEEMNLDFQVLGPMSKNAEEVEVLIVAAKKEKIDDRVAVA
                     EEAGLKVSIMDVEAYATETAYRQILKQLPQHSKKTITMILDIGAHVTHVNVMVGTHSV
                     YAREQAFGGAMLSQEIQRRFGLSPEESEIAKRQGGLPESYEQEVLQPFLQNLAGEAAR
                     AVSFFTNSTQYNKVDHLLLAGGVSATEGLAALIEQKTGVNTLVANAFQGMVLANKIKP
                     SQLATDAPALMIACGLALRGVNA"
     gene            5846..6439
                     /locus_tag="VNZ84_00460"
     CDS             5846..6439
                     /locus_tag="VNZ84_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HWU65448.1"
                     /translation="MIKVNLLPHRQIRRAERQREFGLMASLVAVAAAAILFVSWSYIH
                     NKIQAQQARNQRLQDEMLRLDKEIAVIGTLKEQIKHVLERKQIVEGLQSDRNQAVLIL
                     DELARQLPEGAFLKSVKQLGDEIELKGVADTNARIATLVHNLSDSPIMHSPNLVEIKA
                     NTNALGVKEYEFVLRVSLKREVAQEGADKQKPMPAKG"
     gene            6446..7090
                     /locus_tag="VNZ84_00465"
     CDS             6446..7090
                     /locus_tag="VNZ84_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HWU65449.1"
                     /translation="MQLSDFNNIDLKTAGNLPMPMKAVLLSILAAALLVLGYVFLLSP
                     NLKELKAEKEKEQGLRDEYMAKKIQAVKIKAYEQQVVEIQRTFGTLLKQLPDKAEMDR
                     LLTDINQAGLSQGLSFEGFVPMPEVFAEFYAEKPINIKVLGHYHELGAFVTEVAKLSR
                     IVTLHNLQIQAAGKDDKQPHKDMLVMEAVAKTYRYLDADEVAARKAQESKAGKK"
     gene            7087..7617
                     /locus_tag="VNZ84_00470"
     CDS             7087..7617
                     /locus_tag="VNZ84_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HWU65450.1"
                     /translation="MMRKQTLSSFIPVLLLAACGASQDDDLTKFMQDADQTLVAPVEP
                     LPEVQGFSPKQYNADGVLHDPFVPRKAAVQNTHQPDLNRPKEPLEAYPLENLKFVGVL
                     SKKNSMFATIQTPDNMVYQVRPGSHLGEKFGVVTELAENRHSLKYELKVKETIQDPVS
                     GEWSQQMTTLELQEHQ"
     gene            7617..>8321
                     /locus_tag="VNZ84_00475"
     CDS             7617..>8321
                     /locus_tag="VNZ84_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HWU65451.1"
                     /translation="MKKLTCLVARVAFLSSVLAAPIWGLAAELPASANQLQAVDVTAL
                     PGGRLNVQLQLSQPLASAPLSFTLSNPPRIVFDLPNAVNATGKNVLPVNQGVLKSVQV
                     AQASDRSRVVLNLSKPVQHQLQIQDQALLVGLTQDGADAPVAAAVAAKRFAEPEVAAV
                     HKIQKIDFVRGKNGEGRILVDLSDSRVGVNVRNAGKTVVVDFANTELPEELQRRLNVV
                     NFNTPVLYVDALRHHHQ"
BASE COUNT         2052 a         1952 c         2278 g         2039 t
ORIGIN      
        1 gccccaagtt tccggtatgc aaatgaccat tgcggatatt cgccagcggg ccttgcaaaa
       61 taatttgcaa cttaaggtgg caaagatgga tcctgagctt gccaaacagt cattgcgtga
      121 agagcaagcc aagtttgacc aggtgatctt tgcctatgcc aagtatgcgg aaaaagatac
      181 accggccatg tcgggtgata aggtagtgat caaatcggac aatccgctac tcgatggtga
      241 attggccaag ttgaatctag atgcccagaa aaaggagttt tgggatattg aggccggcgt
      301 aaaagtgcca ttgcgtactg gtggcacggt gacgctgagt gcgccgctag agaaccgaca
      361 gagcctgggt aaatttgcca gtgaccagta ccgtagtgct ttgcgttttt cattcagcca
      421 gcctttactg cgtaatgcgg ggcagcaggt caatgaagcc agcatccgtg ttgcagaact
      481 cgatcgtgac agtgtacaac tgaaaaccag gttgcaggcc atacggattg ttgcgacggt
      541 ggacaaagct tattgggagc tctacgaagc ttgggctgca ttagacgttc ggaaaaacca
      601 gtttgaatat gccagccaaa acctggagat ggtgaaacgc cgtgtacagg aaggcttaac
      661 ggctgctgtg gaagtgaatc gtgcgcaaat tggggtggct gaccggatgg aggcgctaat
      721 tgtcgctgaa accaatctca agctggcgca gcggcaatta caatttttgc tgaatgattt
      781 gcctgatgac caggagaggc aatctccctg ggtaccaaca acggtaccca gtctggtcaa
      841 gtttgaattt gaccgcgcgc acctgatccg tgatgcactc gatagccgta tcgaattgct
      901 tgaacaagaa ctcaagctaa gcgctgacct gatccagatt gattatctgc aaaaccagac
      961 cttgcctttg tttacactgg attatcaata tggtgcgttg tctggatctg gtggtcgatt
     1021 tggcgatatc gggcaagatt tgtttgatgg ccgttaccgt gactggtcgg tcggtttaaa
     1081 gtttgagatg ccagtcagca atgaggctcg taaagccaaa ctggatcgcg cgattgccca
     1141 gcgtatgcag cgattgacca ctaaaacgtt gcaaagcctg accgtcaaac gcgaaattca
     1201 tgatgccctg gataaggtgg agcaaaactg gcaacgcatt attgcagccc gtcagcaggt
     1261 gctggtggcg ggcattaact acgaggctga gctcaagcag tttaatgaag gtctgcgcac
     1321 catgacggag gtgctggaaa cattgacccg gttgggcgag gcgcaactca aggaaattcg
     1381 tgcgatcagt gactatcagg tgtcactggt ggatatggcg tatgcgaccg ggaccgtgtt
     1441 gggacacgga cagatcagct tgcaataggc gttattcgtt gactactttg ccaggaaaca
     1501 gcacgtctga taactgctgt aagttcatgc gttgaatccc tgacttttcc ccgtttttac
     1561 gtggggccgt gcgaatatcg tcggcttcaa acacactgat gcgctgtgtt ccgaaacggg
     1621 tttctaccag caacactggc acccgtttgc gcaccatatc atgcttctgt ttgcccggtc
     1681 gatcagacat tctgaactgt tgttcgtcta gccggtaagg aacattctgg ttaagcaggt
     1741 aaatttctag ttctttggca ctttcagcaa acacgtgaat gtccgtgtct gcataagggc
     1801 cagctgtgcc ttcaagcaca gagccgatta aatgcggatt aaactttttg agtagctgca
     1861 tagtatgcaa ggcagcatgg cgcaaatcga gtaaatgtgc ggcatcggtc tcgtcggcat
     1921 acaaggcatg atattcgcgc aaggcgctat caatttcacc attggttggt agaggggcgt
     1981 cctgatgtat gcccagtaat ttgaccgcct tgcgtttggc acgcccatag tcactgatgc
     2041 cttcttcaaa catcaggcgc gctgcggatt gtgcaatttc ttgccgcagg tcagttggaa
     2101 aatcgtcaaa cataccttgg gcctttgcaa taaagtatct agttagggct tagcaatttt
     2161 gcggccggat ttgagggcag cttcctgtca tcgttactgc ctccgctatg ttcgccaatc
     2221 agtctttcca ggaaagatgg ctcattgtct tcgggcggct gctcaccttc tgggtcaaac
     2281 aggccgggca ggttttctgg tgtcgattga ggcaggtgtt cttcgtaaaa atactcgtag
     2341 atgcccggat catcttcacc gacttgcgcg ccggtatagg cgtctacttt tagttgtacg
     2401 atgccatcag gcactttgta ctggtagaca ggcagcccag gaagggtttg ctgcatataa
     2461 ctaatccaga tcggtaaagc agcacggcca ccggtttcac cgcgtcccag gttgtgcgga
     2521 ttgtcataac cgacccaggc gaccgcgact tcttttgggt gatagccggc aaaccaggca
     2581 tcaaactggt cgtttgtggt accggttttg cctgcaatgt cagggcgtcg cagcgacaat
     2641 gccgccctag ccgtgccatc acgcacaaca tcttgcagca tggaggtaat gatgaaggca
     2701 ttccgtgggt caatgacgcg cgcttgcgac acatgtttgc ccgcctgcac agcttgaatc
     2761 accttgccgc ggctatcgat gatcttttca atcaggtagg ggtgtacctg gtagccgcta
     2821 ttggcaaagg ttgcataggc cgctgccatt tgccagacag aggttgagcc agcacccagt
     2881 gccgttgaca gataggccgg gtggtctttg ggagcgaagc cgaacttgcg gatatagtcc
     2941 tgcgcatagc gtgcgccgat ggcgttcatc aaacggatag aaatcgtatt ttttgactgt
     3001 gccagcgctt cacgcaaacg catcgggcct gcatattttt catcgtagtt acgcggttcc
     3061 cagctgctgt tgctgccggt ttcggtcgag gtaaagctga taggcgcatc ttcgaacacg
     3121 ctggccgggc tgtagccttt ttcgagcgca gcagagtaaa taaatggttt gaagcttgac
     3181 ccaggctggc ggcggccctg ggtgacatgg ttatatttat tgcgctggaa gctaaaccca
     3241 ccaatcaaag cctgtacttg tccggtctct ggattgagcg ccatgaagcc gctttcaact
     3301 tctggtaatt gcaccaccag ccagttgtca ttctgctggt aaacacggat aacagcacca
     3361 ggtaccaatg ataaaggggt gcgtttggct ggtggcatat ggtttttcac tagcaataac
     3421 gatttgtcct ttaaagtcag caccgtaccg cgtttggtga ccaaagtgag caggttgggc
     3481 tcaaccttgg tgaccaaggc gggcacaaat tgattgtagg tgacaaaatc gcgtaacaat
     3541 tcagccgctt cactggcctt caatttaccc gctggcaagg aaatgatttg ttcggcacca
     3601 cggaaaccgt gcctcaattc aaagttgata atgccctgaa tgacagcgtt attggctgct
     3661 gcctgatgtt tgttgttgat ggtggtgtaa acacgcaggc cactggtata agccttctca
     3721 ccataacgct tatacaggct tgccctgaca atttctgtaa catactcact gcccagtact
     3781 tgaatttgcc gcctggatga ctggaatttt aatggcgtat tcatcgcctg ttctagctcc
     3841 tgtgtcgaga tcagtccata gcgatgcata tcgcgtagta cctcgtgctg gcgcctgatg
     3901 gcatagtcag gatggatata cggatcgtat ttgctcggcg ctttgggcag accagccagc
     3961 agggcggctt ctgccaggtt gagttgcttt aagtctttat caaaatacgt cttggaggct
     4021 gttgcaaagc cataagcgcg ctcacccagg taaatctggt tgaggtaaag ctccagaatt
     4081 ttgtctttgc tgatagcatt ttcaatcttg atagccagca tggcctcttt gatcttggtc
     4141 cagatattac gcggtgcatc ggggccacta aaaaagtttt tggcaacctg catggtgatc
     4201 gtactagcac cttcatggcc tcgcccggtg aggttgttgc ggatggcgcg caggatgcct
     4261 gtcgtatcaa taccgttatg ttggtaaaaa cggcggtctt caatcgccag gatggcgtct
     4321 ttcatgacct tgggtgtttg ctcaatcggg acaaaggcgc ggcgttcctg gccaaactcg
     4381 gcaatcaggc cgccttgggc atcataaatg cgcaacggca atttcgggtg gtagtccgtg
     4441 agtgcgtcca gtgacggcaa tgatgggtag agaatactga tcactaacac cagcagggcg
     4501 gcggcgatca ggctgccggt aatggatagg aaaagaaggg actgcaacca gtttcttgaa
     4561 ttcatgcttc ttgctgccat cgtgagaatt acctatgaat tgtaatcttt ttttgctgat
     4621 tcattggcag ttaattatgg aatcatgcaa ataatcagca ttcttctggc tgttatctgg
     4681 cttgggaaat tgcatgcaag gcatgaagcg cgtgagttgg catggttgat gcatgcaccc
     4741 acggggattt aaataattca aaaagggcaa taccgttgag tctgggcttt ttacaacgca
     4801 aaaaaaccag ttttattggt gtagatgtca gtgcaaccgc cgttaagatg gtggagttga
     4861 gcgctgtcgg taagcaacag ttccgtctgg atggctatgc caatgtgacc ctgcccaaag
     4921 gggtcatcag cgatggcaat atcaatgacc tgacgcaggt ggccgagacg ctccgttctg
     4981 cctggcgttt gctgggaacg cgcgcgcgtc atgcggtgct cgcattgcca acggctgccg
     5041 tcatttccaa gcgcgtgatt atgccggccg gtatgagcga agaagattta gagttgcagg
     5101 tggaatctga agccaatcag tatattccgt ttccgctcga agaaatgaac cttgactttc
     5161 aggtgttggg acccatgagt aaaaatgccg aagaagtgga agtgctgatt gtggcggcca
     5221 aaaaggaaaa gattgacgat cgcgtggccg ttgcagagga agcgggacta aaagtcagca
     5281 tcatggatgt agaagcctac gcgacagaga ctgcctatcg acaaatcctc aaacagttac
     5341 cgcaacacag taaaaaaacg atcaccatga ttctcgatat tggcgcacat gtcacgcatg
     5401 tgaatgtcat ggttggtacc cattcagtct atgcccgtga gcaagcgttc ggtggtgcga
     5461 tgttatcaca ggaaatccag cgccgtttcg gcctttcgcc agaggaaagt gaaattgcca
     5521 agcgtcaggg tggcttgcca gaatcctatg agcaggaggt tttacaacct ttcttgcaaa
     5581 acctggcggg cgaggctgca cgagctgtct ctttctttac caactccacc caatacaaca
     5641 aggtcgatca tctgctgctg gccggtggcg tctccgcaac tgagggtttg gctgccctca
     5701 tcgaacaaaa aaccggtgtg aataccctgg tcgccaatgc cttccagggc atggtgctgg
     5761 ccaacaaaat caaaccttcc cagctggcaa ccgatgcgcc agcactgatg attgcctgtg
     5821 gtttggcgtt gcggggggtg aatgcatgat taaagtcaac ctgttgccgc atcggcaaat
     5881 caggcgtgcc gaacgccagc gcgagttcgg cctcatggct tcattggtgg ccgttgcggc
     5941 ggcagccatc ctgttcgtga gctggagcta tatccacaac aaaattcagg cccaacaggc
     6001 gcgtaaccag cgattgcagg atgaaatgct acgcctggac aaagagattg ctgtgattgg
     6061 tactttgaaa gagcaaatta aacatgtact tgagcgtaaa cagattgtgg aaggtttgca
     6121 gagcgaccgt aaccaggcgg tgttgatttt ggatgagctg gccagacagc taccagaagg
     6181 agcctttctc aaaagcgtca agcaactggg agacgagatt gagctcaagg gagtggcgga
     6241 taccaatgcc cgtattgcga cgctggtaca taacctcagt gactctccta ttatgcacag
     6301 tcctaacctg gttgaaatca aggcgaacac caatgcgctg ggcgttaaag aatatgagtt
     6361 tgtattacgg gtgtcattga agcgtgaagt cgctcaagaa ggcgccgaca agcagaagcc
     6421 catgccagcc aaaggataac caagcatgca actttctgat tttaataata ttgatctaaa
     6481 aacggccggt aatctaccga tgccaatgaa agcggtattg ctgtctatcc tggcagcagc
     6541 attgctggtg ctgggctatg tgttcctgtt gagccccaac ctcaaggagt tgaaggctga
     6601 aaaggaaaag gagcaaggct tgcgggatga atacatggct aaaaaaattc aagccgtgaa
     6661 gatcaaggca tatgagcaac aggtggtgga gattcaacgt acctttggta ctttgctcaa
     6721 gcagctgcca gataaagccg agatggatcg cttgctgacc gatattaacc aggcgggatt
     6781 gagtcagggc ctttcgtttg aaggctttgt gcctatgccg gaagtattcg ccgagttcta
     6841 cgctgaaaag cccatcaata ttaaagtgct gggccactat catgagctgg gcgcatttgt
     6901 gactgaggtc gccaaactat cccgtattgt gaccctacac aatctgcaaa tccaggctgc
     6961 cggtaaagat gacaagcagc cgcacaagga catgctggtc atggaggctg tcgcaaaaac
     7021 ctaccgttac ctggatgcgg atgaagtggc tgcacgcaaa gcacaagaga gcaaggcggg
     7081 gaaaaaatga tgcgtaaaca gaccttgtct tccttcattc cggtactgct tctggcagcc
     7141 tgtggggcat cacaggatga tgatctgacc aagtttatgc aggatgcaga tcaaacgctg
     7201 gttgctccgg tagaaccctt gccggaagtg cagggctttt ctcccaagca atataacgct
     7261 gatggtgtgt tgcacgaccc gtttgtgccc cgcaaggcgg cagtacaaaa cactcaccag
     7321 cctgacttaa atcgccccaa ggagccattg gaggcatatc cactggaaaa ccttaagttt
     7381 gtaggggttc tgagcaaaaa gaacagcatg tttgccacca tacaaacacc agacaacatg
     7441 gtgtaccagg tcagacctgg ttcacatttg ggtgaaaaat ttggggtggt cactgaactg
     7501 gcagaaaacc gccatagctt gaaatacgaa ttaaaagtaa aagagacgat acaagacccc
     7561 gtcagtggtg agtggtctca gcaaatgaca acattggaat tgcaggaaca tcaatgatga
     7621 aaaaattaac atgccttgtt gcccgcgtgg cattcttgtc gtcagtgttg gcggcaccca
     7681 tatggggtct cgcggctgaa ttgccagcct cagccaacca gttgcaggcg gtcgatgtga
     7741 cggcgttgcc cggtggccgg ttgaatgtgc aactgcaatt gtcccagcca ttggcctctg
     7801 cgccactcag cttcaccttg agcaatccgc ccaggatcgt gtttgatctg cccaatgcgg
     7861 tgaatgcaac cggcaagaat gtgttgccag taaaccaagg tgtcttgaag tctgtgcaag
     7921 tggcacaggc gagtgaccgc agccgtgtcg tgttgaactt gtccaagccc gtacagcatc
     7981 aattacagat ccaagaccag gcattactgg ttggattgac acaagacggt gctgatgcgc
     8041 cagttgcagc tgcggtagct gccaaacgtt ttgctgagcc tgaagtcgcg gcagtacata
     8101 aaatccagaa gatagatttt gtgcgcggca aaaatggtga gggtcgtatt ttagtggatt
     8161 tgtctgatag ccgcgttggc gtcaatgtgc gtaatgctgg taaaaccgtg gtggtagatt
     8221 ttgccaacac tgagctgcca gaagagctgc agcgccgttt gaacgtggtg aatttcaata
     8281 ccccagtgtt gtatgtggat gccttacgcc atcatcatca g
//
LOCUS       DATMSK010000015         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_306136,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000015 DATMSK010000000
VERSION     DATMSK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306136"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1098)
                     /gene="apbC"
                     /locus_tag="VNZ84_00480"
     CDS             complement(10..1098)
                     /gene="apbC"
                     /locus_tag="VNZ84_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HWU65452.1"
                     /translation="MAISELLIQSTLKLCIDPNTGKDFISGKSIKKIQVSGNDVSLDV
                     VLGYPAQTQLDAIRQQVSEALASIEGIGKTTVNVGCRIVAHSVQRGVNLLPNVKNVIA
                     VASGKGGVGKSTTSVNLALALAAEGATVGLLDADIYGPSQPQMLGISGRPDSADGKSI
                     EPMRAHGIQAMSIGFLVDTDTPMVWRGPMVTGALEQLLRDTRWQDLDYLVIDLPPGTG
                     DIQLTLAQKIPVTGAIIVTTPQDIALLDARKGLKMFEKVGIPILGIVENMNTHICSNC
                     GHEEHIFGAGGGAVMAKDYNVDLLGSLPLDINIRMQADGGQPTVVAAPDSAISKTYRE
                     IARKAASKIAIASLDHSAKFPNIVIQKT"
     gene            complement(1144..2187)
                     /locus_tag="VNZ84_00485"
     CDS             complement(1144..2187)
                     /locus_tag="VNZ84_00485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWU65453.1"
                     /translation="MLIVTDTFQQTNGVSTTYKNLRRVAKSRNLRFRVLHPSLFRWMP
                     LPFYPEIQLNLQPFRLWRTLNRMQPTQLHIATEGLMGLVARCWCKLHQKPYTTAYHTR
                     FPEYLALMWRIPARWTYAYLRWFHRGALTTFVTTESMRQTLMKQGFHHLVIWSRGVSE
                     QLMSASPETSSASKLRVLNVGRVSKEKNLDALCVHQDEFEITIVGDGPYLNELRQKYP
                     RVHFLGYRYGQALAELYTQNDVFAFPSRTDTFGIVMIEAMCNGLPVAAFNVTGPCDVI
                     EQGFSGVLHHDLQQAILRCQSLDRQAIRLKARGLWSWSHCFDTFHHHYQKSAERTTHL
                     PCQKQHDERTSLR"
BASE COUNT          536 a          560 c          596 g          573 t
ORIGIN      
        1 atggaatggt tacgttttct ggatcacaat gtttgggaat tttgcactat ggtccagact
       61 ggcaatcgcg attttgctgg cggctttacg tgcaatttcc cggtaggttt ttgaaatggc
      121 gctatccggt gcagccacta cggtgggttg gccgccgtcg gcttgcatgc ggatattgat
      181 atccagcggt aagctaccca gcaagtccac attgtaatct ttcgccatga ctgcgccacc
      241 acccgcacca aaaatatgtt cttcgtggcc gcagttggag caaatgtggg tgttcatgtt
      301 ttcgacgatg ccgagaatcg ggatgccaac tttctcaaac atcttcaagc ctttacgcgc
      361 gtcaagcaag gcaatgtctt gtggggttgt gacaataatc gcacccgtca caggaatttt
      421 ttgcgccagt gttaattgaa tatcacctgt acccggtggc aggtcaatca ccaaataatc
      481 caggtcttgc cagcgtgtat cgcgtaacag ttgttctaaa gcgccagtca ccatggggcc
      541 gcgccagacc atgggcgtat cagtatccac caggaagcca atactcatcg cctgaatgcc
      601 atgcgcacgc atcggttcta tgcttttgcc atctgcactg tccgggcggc cagaaatgcc
      661 gagcatctgc ggttggcttg ggccgtaaat gtcggcatcc agcaagccta cggtagcccc
      721 ctcggcggca agcgccaggg caaggttgac agaggtggtt gatttgccga caccaccttt
      781 accagaggca acggcgatca cgtttttcac attcggcagc aaattgacgc cacgttgcac
      841 gctgtgcgca acgatgcggc aacccacatt gacggtcgtt ttgcctatgc cttcaatact
      901 agccaaggct tcactcactt gctggcggat ggcgtccagt tgggtttggg cagggtagcc
      961 tagcaccaca tcgaggctga cgtcgttgcc gctgacctga atttttttaa tggatttgcc
     1021 actgataaaa tctttacccg tattcgggtc tatgcacagt tttaaggtgc tttgaatgag
     1081 taattcgctg atggccatgc ctgagattcc tgccgtaatg cctgactaat tagatgaggt
     1141 attttaccgc aatgatgtgc gttcgtcatg ttgcttctgg cagggaaggt gagtcgtgcg
     1201 ctcagcagat ttctggtagt ggtggtgaaa ggtatcaaag cagtgagacc agctccagag
     1261 gccgcgtgct ttaaggcgga tcgcttgcct gtccagtgac tggcaacgca agatggcctg
     1321 ttggagatcg tggtgcagca cgccggaaaa gccttgttcg atcacgtcac acgggccggt
     1381 gacgttgaaa gccgccacgg gcaagccatt acacatagcc tctatcatca caatgccaaa
     1441 agtatcagtg cgcgaaggga aggcaaacac atcattctgt gtatacaact cggctaatgc
     1501 ctggccatag cggtaaccca ggaaatgaac acgtggatat ttttgcctta actcatttaa
     1561 gtaaggacca tcaccaacga tagtgatttc aaattcgtcc tggtgcacac acaaggcatc
     1621 taaattcttt tctttactca cacggccgac attgagtaca cgtagtttgc tagcggatga
     1681 tgtctctggt gatgcactca taagttgctc tgacacaccg cgcgaccaga tcaccagatg
     1741 atgaaaccct tgcttcatta atgtctgtcg catgctttcc gtggtgacaa acgtggtcaa
     1801 cgcaccgcga tgaaaccagc gtaaataagc ataagtccag cgtgctggaa tacgccacat
     1861 cagtgccagg tattcgggga aacgtgtgtg ataagccgtg gtatatggtt tttgatgcag
     1921 cttgcaccag catcgtgcca ccaggcccat caagccttca gtcgcgatat gtagttgtgt
     1981 tggttgcatg cgattgagcg tgcgccacag cctgaaaggt tgaagattga gttgtatttc
     2041 cggataaaac ggcaacggca tccagcgaaa caggctggga tgcaagaccc tgaagcggag
     2101 gttgcgcgac tttgcgacac gccgcaggtt tttataagtg gtcgaaacgc cattggtttg
     2161 ctggaaggtg tcggtcacaa tcaacattac tattacgctc caaatagtct ggttgaacta
     2221 tcttgccaac gctgcgtgac gggtttgtga acatgaacat ttaaa
//
LOCUS       DATMSK010000016         4455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_423519,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000016 DATMSK010000000
VERSION     DATMSK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423519"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VNZ84_00490"
     CDS             complement(<1..322)
                     /locus_tag="VNZ84_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HWU65454.1"
                     /translation="MLSVCRTSLFFLLSMGIAQAGTEQVRETKSELIFNLALLSQAPN
                     PRAQDTYVICAFEDDIGYLKATALESQKLQNLQIFLVTVKTATDVKRCNLLYIQDYIP
                     QKPLE"
     gene            complement(563..2068)
                     /locus_tag="VNZ84_00495"
     CDS             complement(563..2068)
                     /locus_tag="VNZ84_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWU65455.1"
                     /translation="MAASLSQEINTLIQQLNQGQFEAVIQRARPLAVQHPREFILHHL
                     LALALDQTQQYASAAQAYEKALQLNPNAAELWFNYAIVQTQLNALPAAEQAYRKAIAL
                     NPQFFEAHGNLGTVLQKQGRLDEATASYQQGLRIQPQDARGHFNMGTVLRDKGQLQAA
                     VQSYERAIALFPNYTDAYNNLGETWRDQGDMTKAVQYYQEALQRNPQHPGANYNMGEF
                     LYLAKRFEEAIPHFQASQLDDWQSRVLYCLYRAEKFAEFKQLRHEILNQGPHTAPFIA
                     TLSTHYSLNHGEPDPYQFCPDSLSFVYHRSLPELAPGSALLTELLENIAQADIAERKQ
                     ARLTNGKQSAGNLFKRPEAAFRALGELVRREFGQYHAQFKQADCALITHFPETLEFTS
                     SWYVKMQKGGHLDAHIHEIGWISGAVYLAMPETSDPKEGAFEYGTHGDQYPQLHDNFP
                     TRFIKPNVGDIVLFPSSLFHRTIPFNADAERICVAFDLKPPAHLATARSQY"
     gene            complement(2168..3469)
                     /locus_tag="VNZ84_00500"
     CDS             complement(2168..3469)
                     /locus_tag="VNZ84_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HWU65456.1"
                     /translation="MQPALWDALYQQWQSAKERLLAYRYCTPWFVIHTTWQGVTKHRL
                     MGLAAALSFYALFALIPLIVLIFFLISHLVYSSDYAIVKLAILTSNLLPEFSQRIMIE
                     VYNNTRTKVAWGVVGFLILLWTITPLAAHMRSSFHLIASRNDVASFWHKKFRDVVAVL
                     GVLLVFFLFTAAGFVLESIIVFLSNHLPSAWTNDLGSLLSLALTTLLIAGFYRTFCPL
                     TVSWRHLLLSALLTASVWMLMRPAFGLFLSVNKNFGAVFGSMKALFVSISWLYLNFAV
                     FLIGIELMVTLKQKEVLLLKGLFDDIPNQQHYLQALMQTYGKTYIQGEHVFREGDAGH
                     HYYMVVDGTLHLTQQDSDGSEKTLRILQYGDFFGDISLFSAQPVTASAIVVSPTASVI
                     EIVAECMENLLLEDPNIAVRLLKQLSHHAQIKPALQQSTTY"
     gene            complement(3494..4444)
                     /gene="pip"
                     /locus_tag="VNZ84_00505"
     CDS             complement(3494..4444)
                     /gene="pip"
                     /locus_tag="VNZ84_00505"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HWU65457.1"
                     /translation="MTATSLYPECQPYEHALIPVSSKHSIYVEQCGNPEGVPVIFLHG
                     GPGSGCNPGQRRFFDPDHYRIILFDQRGCGSSQPAGEIKENTTQDLIADIETIRHQLQ
                     IDRWHVFGGSWGSTLALAYANSHPTPVISLALRGIFLSRRQEIDWFLGQVALFFPEAW
                     QTLLMPVPASHRENVLDYYAKQVFQEDTSISIPAAIRWNAFESSIMSLIPKTDSSEVN
                     GAVELARARVQIHYIQHLCFIDGDQLLQTAPTRLAAIPTTIVQGRYDMVCPPQTAWEL
                     AQALPHATFIVVADAGHSAMEPGTCQALLSATEQYKRLTP"
BASE COUNT         1130 a          994 c         1167 g         1164 t
ORIGIN      
        1 attctagcgg cttttgcggg atatagtctt gaatatataa cagattgcag cgtttaacgt
       61 ctgtggctgt ttttactgtg accagaaaaa tctgtaaatt ctgcagtttt tgtgattcca
      121 gcgccgtggc ttttaagtag cctatatcat cttcaaatgc gcaaataaca taagtgtctt
      181 gcgcccgggg attcggtgcc tggctgagga gagccaaatt aaaaatcagt tcgctttttg
      241 tctcacgtac ctgttctgtg ccagcttgag caatgcccat gctaagcaaa aaaaataaac
      301 ttgtgcggca aactgaaagc atattgatct gaccccatca agtgagttga agtaatcgtg
      361 cgctgaggat ggctttggtt cagtaattgg ggagcaagat catttaaatg tttcttgcca
      421 gacccttaag aatagctgtt tcctcaataa ggcatcatac cttgcgccat agaatctaca
      481 atcggcaaaa ttgataaatt ctgtatcagt ttgataaggt gctctgctgt ctgaaagcgc
      541 agtaagttgc ggtgggtgat ttttagtact ggctacgtgc ggtagcgaga tgtgctggag
      601 gttttaagtc aaatgctaca caaatgcgct ctgcatccgc attgaaggga atcgtcctgt
      661 ggaacagtga ggatggaaac agtacgatgt cgcctacgtt cggcttgatg aagcgggtgg
      721 ggaaattgtc atgtaattgt ggatactgat caccgtgagt accgtattca aaagcacctt
      781 ctttcggatc cgaggtttct ggcatcgcca ggtaaacggc accacttatc cagccgattt
      841 catggatatg ggcgtcaaga tgccctccct tttgcatttt gacataccac gagctggtga
      901 actccagcgt ttccggaaag tgcgtgatca gtgcacaatc ggcttgcttg aattgtgcgt
      961 ggtattggcc gaactcgcgc ctcacaagtt ctcccaaggc tctgaaggcc gcttcaggcc
     1021 gcttgaataa gttgccggcg gattgtttgc cgttggtgag gcgtgcctgt ttacgctcag
     1081 cgatgtctgc ttgcgcaatg ttttccagta actccgttaa caatgcgctg ccgggtgcca
     1141 gctctggcag gctgcggtga tatacaaatg acaggctatc cgggcaaaat tggtaaggat
     1201 ctggttcgcc atgattgagg ctgtaatggg tggacagcgt tgcaataaat ggtgcggtat
     1261 gcggaccttg attgagaatc tcatgacgta gttgcttgaa ttctgcaaac ttctctgcgc
     1321 ggtataaaca ataaagtacg cgtgattgcc aatcatccag ctgggaagcc tggaagtgag
     1381 gaatcgcttc ttcaaagcgc ttggccagat acaaaaattc gcccatgttg taattagcac
     1441 cagggtgctg cggattgcgt tgcagggctt cttgataata ttgcaccgcc ttagtcatat
     1501 cgccctggtc gcgccaggtt tcgcccaggt tgttgtaagc atcggtgtag tttggaaata
     1561 gtgcgatcgc gcgctcataa ctttgtacgg ctgcctgcag ctgtccttta tcgcgtaaca
     1621 ccgtgcccat attgaagtgc ccccgtgcat cctgtggctg gatacgcaag ccctgctggt
     1681 aactggctgt ggcttcgtcc agtcggccct gtttctgtag cactgttccc aggttgccat
     1741 gtgcttcaaa aaattgcgga ttcagtgcaa tcgctttgcg gtaagcctgt tcggcggcag
     1801 gcagtgcatt tagctgcgtt tgtacaatgg catagttaaa ccatagttct gctgcatttg
     1861 ggttcagctg cagggctttt tcataggctt gtgcagcaga tgcatactgc tgtgtctgat
     1921 ccagcgccag cgctaacaaa tggtggagga taaactctct gggatgctgt acagctaatg
     1981 gtcgggcacg ctgaatcacg gcttcaaatt gaccctgatt cagttgttga atcaatgtat
     2041 taatttcttg tgaaagggag gcagccatga tgcaaaattc caaatatagt tacaattttg
     2101 taacaaatat tacaatttcg caatgattgt ctgtgtggat cgagtaaacg tgtcgttgtg
     2161 agtactatta gtaggtggtt gattgctgta gtgcaggctt tatttgagcg tgatgggaaa
     2221 gttgttttag caaacgcacg gcaatgttag ggtcttcgag taagaggttt tccatgcact
     2281 cggcgactat ttcaatcacg gatgctgttg gcgaaaccac tatcgcactc gccgttactg
     2341 gctgcgcaga gaatagcgaa atgtcgccga aaaagtcgcc atactgcaag atgcgtaatg
     2401 ttttttcgct gccgtctgaa tcttgttgag tcaaatgcag cgtgccgtcg acgaccatgt
     2461 agtaatgatg gcctgcatcc ccttctctaa acacatgttc accttggata taggttttgc
     2521 cgtaggtttg catcagggct tgcaaataat gctgttgatt agggatgtca tcaaataagc
     2581 ctttgaggag tagcacctct ttttgcttaa gggtcaccat caactcaatg ccaatcagga
     2641 ataccgcaaa attcaggtaa agccagctaa tagacacaaa caaggctttc atgctgccaa
     2701 acacggcgcc aaaattcttg ttcacactta aaaataaacc aaaagctggc cgcatcagca
     2761 tccatacgct ggcagtcaat aacgcagaaa gtaataagtg gcgccaactc acagtgagtg
     2821 ggcaaaaggt gcgatagaaa ccggcaatca acagtgtagt caaggcgagt gataacaagg
     2881 agccgaggtc attggtccat gcagagggta agtggttgga taggaaaaca ataatcgatt
     2941 ccagtacaaa gcctgcggca gtgaacagga agaagactag caatacgccc aatacagcca
     3001 ccacatcgcg aaacttttta tgccaaaacg aagcaacatc attgcggctt gcgatcaggt
     3061 gaaaactgga gcgcatgtgc gcagccaaag gcgtaatcgt ccatagcaaa atcaaaaagc
     3121 caaccactcc ccaggctact ttggtacgcg tattgttgta aacctcaatc atgatgcgct
     3181 ggctaaattc gggtaacagg ttgctggtga gtatagccag cttcacaatg gcataatcag
     3241 atgagtacac caagtggctg attaaaaaga aaatcagcac gatgaggggg atcagtgcaa
     3301 acaaggcgta aaaggagagt gccgcagcca gacccatcag tcggtgtttt gtgacgccct
     3361 gccaggttgt gtgtatgaca aaccagggtg tgcagtagcg ataggccagt agccgttctt
     3421 tggcactttg ccattgctga tataacgcgt cccataatgc aggttgcata tgctgtttct
     3481 acgatatcaa tgactatggc gtgagccgct tgtattgctc agtggcactc agcagggctt
     3541 ggcaggtgcc gggttccatt gcagagtgac cagcatccgc taccacgatg aacgtggcat
     3601 gaggcaatgc ctgcgcgagt tcccatgcag tctgtggggg acataccatg tcatagcgcc
     3661 cttgcacaat cgtcgtggga atcgcagcca gtcgagtcgg ggctgtttgt agtaattggt
     3721 caccatcaat aaagcataaa tgttgtatgt aatggatttg tactcgcgcg cgtgccaatt
     3781 cgacagcacc atttacctcg ctgctgtcag ttttaggaat caggctcatg atgcttgatt
     3841 cgaaggcatt ccagcggatg gccgccggaa tactgataga ggtatcttct tgaaacacct
     3901 gcttggcgta gtaatccagg acattttccc ggtggctggc tggtaccggc atcagtaaag
     3961 tctgccatgc ctctgggaaa aacagggcta cttgtccaag aaaccagtcg atctcctggc
     4021 gcctacttaa gaaaattccg cgcaaggcca gactgatgac gggggtgggg tgactattgg
     4081 cgtaggctag ggcgagcgtg ctgccccagg agccaccaaa tacatgccaa cggtcaatct
     4141 gtaactgatg gcgtatggtc tcaatatctg cgatcaggtc ttgagtggta ttttccttga
     4201 tttcgcccgc aggctggcta ctgccacaac cacgctgatc aaataggata atgcggtaat
     4261 gatcaggatc gaagaagcgc ctttgccctg gattgcatcc actgcctggg ccgccatgca
     4321 aaaaaatgac aggtacccct tcagggtttc cacattgttc aacatagatg gagtgcttgg
     4381 aactgaccgg aatcagggcg tgctcatagg gttggcattc gggataaaga gaggtggcag
     4441 tcataaaatg ttaat
//
LOCUS       DATMSK010000017         5100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_235828,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000017 DATMSK010000000
VERSION     DATMSK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235828"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VNZ84_00510"
     CDS             <1..169
                     /locus_tag="VNZ84_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HWU65458.1"
                     /translation="LNKQLYKQGLKRMFLHSSETRIAHPLTGEPLHLVAPLPVELEQF
                     LQQQRQLQLVK"
     gene            222..875
                     /locus_tag="VNZ84_00515"
     CDS             222..875
                     /locus_tag="VNZ84_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWU65459.1"
                     /translation="MKQFDLVVWDWDGTIADSTGLISQALMKAAEQAGLPELSETRAR
                     SIIGLGLRESIEVLYGDIPEFQAQALATNYNRNYYANENNILLFAGIAELIASIDKKG
                     CKQAVATGKGRRGLNTALQLSGLSRCFNATKTVDECFSKPHPQMLDELMDELVTMPER
                     TLMIGDSQYDIQMGKNAGVVTAAVSYGSQTADHLQQYSPDYMFHDVATLSAWLLEQT"
     gene            872..1219
                     /locus_tag="VNZ84_00520"
     CDS             872..1219
                     /locus_tag="VNZ84_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWU65460.1"
                     /translation="MSDAVTFNSEELHEKGDGLRFAVPVLGPHATGFVVRFEGKPYAY
                     VNRCAHVPVELDWEHGKFFNMTQDWIICATHGAMYSPQTGHCVMGPCQGKSLSSIPVT
                     EEDGVIMVHLDQF"
     gene            1302..2282
                     /locus_tag="VNZ84_00525"
     CDS             1302..2282
                     /locus_tag="VNZ84_00525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HWU65461.1"
                     /translation="MQEEHQQPSDSKSSSQPPDWAVHTLEKIALAGLQEQKTARRWAV
                     FFKSLSFIFLLLLLLMVLGVIGQNKNASTAHTALIDIAGVIEPGGQVNADAVISSLND
                     AYDSKGTKGIILRINSPGGSPVQAGIINDEIHRQRKLHPEIPVYAVVEDICASGGYYI
                     AVAADKIFVDKASIVGSIGVLMDGYGFTELMKKVGVERRLLTAGSNKGMLDPFLPVNP
                     KQQAFAQSMLDQIHQQFITVVREGRGARLKENEETFSGLFWNGEESIKLGLADAQGSA
                     EYVAREVIKQADIVDFTYQDTVVDRFAKKLGASMAQELGLNAKGLMPSLR"
     gene            complement(2343..3776)
                     /gene="der"
                     /locus_tag="VNZ84_00530"
     CDS             complement(2343..3776)
                     /gene="der"
                     /locus_tag="VNZ84_00530"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWU65462.1"
                     /translation="MLPTIVLVGRPNVGKSTLFNRLTKTRDALVADLPGLTRDRHYGR
                     GLGASQPYLVIDTGGFEPTADTGILKEMAKQTLLAIDEADVIIFLVDARAGLTPQEFT
                     IAQTLRKAQRPVLLAVNKTEGMRKAIVSADFHELGMGEPLSISSAHGEGVRDLIELAL
                     ETISDKEIENSEPVNPDNPRIAIVGRPNVGKSTLVNALLGEDRVIAFDQPGTTRDSIE
                     IALEKNGKQYTIIDTAGVRKRGKVFEAVEKFSVIKTMQAIEDANVAILVVDAKEGITE
                     QDAHVAAYIVDAGRALVVAINKWDGLQDDEREWIKREIDRKLQFLDFAKFHYISALRK
                     KGLNELLGSVDVAFKAAMAKLATPQLTRVLGEATTQHQPPISKGIRPKLRYAHQGGTN
                     PPIVVVHGNHIDGVKASYTRYLEGIFRKAFQLVGTPLRVQYHQGENPFDKEEERKKGE
                     GLVTMRRRKNEMRAKMKQRNEQNKTKK"
     gene            complement(3776..4990)
                     /gene="bamB"
                     /locus_tag="VNZ84_00535"
     CDS             complement(3776..4990)
                     /gene="bamB"
                     /locus_tag="VNZ84_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HWU65463.1"
                     /translation="MKFSFSALRNQPQSLKHVTLAALCLLGSGLSACTAFNDVKTNLS
                     ESIFGAEPANPPVELEDIKSSYEMRVLWSTDVGEAGRFTYRPVLADNLIYAVNAEGSI
                     RQLSAETGKTVWETELGEPISSGAGLGGGLVLVGTSKGHLYALNVNGKKLWSAILSSE
                     IQGQPRYYDGTVIVRTSDHHIYGIDAADGRRKWSYERATPSLSLKTQAGIVVDGGAVY
                     AGFPGGKLIAIRADNGKLVWETTVAQPKGVTEIERIADITSLPFVDGPLVYVVAYQGK
                     VAAVDRQRGQIVWNRDISSYVGLTAENNKVYLSHTIGSVYSLDANNGKTFWRQGNLGY
                     RNLTVPLPLSNVVAVGDLEGYIHLLSQDDGSFVGRTQLGSKPIMSLIADSKPNQFFAQ
                     SRDGHLYAVTYK"
BASE COUNT         1250 a         1179 c         1315 g         1356 t
ORIGIN      
        1 actgaataaa cagttataca aacagggttt gaagcgcatg tttttgcatt ccagtgaaac
       61 tcgtattgcg catccattga ctggcgaacc tctacatctg gttgcgccgt tacctgtaga
      121 gctagaacag tttttgcaac aacaacgtca acttcagttg gtaaagtagt aaatacagca
      181 tttattgatg tctcaaaaat agcatttagg aaagtcactc agtgaaacaa tttgatttag
      241 tagtttggga ttgggatggc acgatcgctg actctaccgg cctgatttca caggcgttaa
      301 tgaaggctgc cgagcaggcc gggttgcccg aactcagtga aacgcgtgca cgcagtatta
      361 ttggtttggg cttgcgagag tcgatagaag tactttatgg tgatattcct gaatttcagg
      421 cacaggcgct cgctacaaac tacaaccgta actattatgc gaacgaaaat aatatattac
      481 tgtttgctgg tattgccgag ctcattgcca gcatagataa aaagggttgt aaacaggcag
      541 tggcaacagg aaaagggcgg cgagggttaa atacggcttt gcaactttca ggattatcca
      601 ggtgtttcaa tgccaccaaa acggtggatg agtgcttttc caaaccgcat ccacaaatgc
      661 tggatgaact catggatgaa ctggtgacta tgcctgagcg tacgctcatg ataggcgatt
      721 cgcaatatga catacaaatg ggtaaaaatg ccggcgtagt cacagctgcc gtcagttatg
      781 gctcgcaaac agctgaccat ttacagcagt attccccgga ttacatgttt catgatgtgg
      841 caacattaag cgcctggtta ctggagcaga catgagtgat gctgtgacat ttaacagtga
      901 agagttgcat gaaaaagggg acggcttgcg ttttgctgtg cccgtgcttg ggccgcatgc
      961 gactgggttt gtcgtccgct ttgaaggcaa gccttatgct tatgtgaatc gttgcgcaca
     1021 tgtaccggtg gaactggact gggaacatgg caagtttttt aatatgaccc aagactggat
     1081 tatctgcgct acacatgggg ccatgtattc gccacaaacc gggcattgcg tcatggggcc
     1141 atgccagggg aagagcttgt catcaattcc agtgacagag gaagacggtg tcatcatggt
     1201 ccatctggat cagttttaag gcgatatacc ggttaaaaat agagcgttag taacccgata
     1261 aaattgttag aataaccaaa actattgacg aaagttgaat tatgcaagaa gaacatcagc
     1321 agccatctga ttcaaaatcc agctcacaac cgcctgattg ggcagtgcat actctagaga
     1381 aaattgcctt agctggatta caggaacaaa aaacggccag acgctgggcg gtttttttta
     1441 aatccttgag ttttattttc ctgctcctct tgctgctgat ggtgctgggg gttatcgggc
     1501 agaacaaaaa tgcgtctacc gcgcatacgg ccttgattga tattgcaggt gtgattgagc
     1561 ctggcggcca ggtgaatgcc gatgccgtga tcagcagcct aaacgatgcc tatgatagca
     1621 aaggtaccaa aggcatcatc ttgcgtatta atagccctgg tggcagccct gtgcaagccg
     1681 ggattatcaa tgatgaaatt caccgccaac gcaaactgca tcccgagatc ccggtctacg
     1741 ccgtggtcga agatatttgt gcttccggcg gatattatat tgccgttgct gctgacaaga
     1801 tttttgtcga caaagccagt atcgttggtt ctatcggggt gttaatggat ggttatggat
     1861 tcactgagct catgaagaag gtcggcgttg aacgtcgttt gcttacagca ggttccaata
     1921 aaggcatgct ggatccattc ttgccagtta atcctaagca acaagcattt gcacaaagta
     1981 tgttggacca gattcaccag caatttatca cggtcgtgcg ggagggcagg ggtgcgcgtc
     2041 tgaaagaaaa tgaagaaacc tttagcggtt tgttctggaa tggtgaagaa agtatcaagt
     2101 tgggtctggc agatgctcaa gggagtgccg aatatgtcgc acgcgaagtc attaagcagg
     2161 cggatattgt tgattttact tatcaggata ccgttgttga ccgcttcgca aaaaaattgg
     2221 gcgccagtat ggcacaagag ttgggcttga atgcgaaagg cttaatgcca tccttaaggt
     2281 agtagaaaag cgcaatagaa aaaggggcag tcatgcccct tttttattta gtgtgttatc
     2341 agttattttt tagttttatt ctgctcgtta cgctgcttca ttttggcgcg catctcgttc
     2401 ttacgacgac gcatggtcac caggccttcg ccttttttgc gctcttcttc tttatcgaac
     2461 gggttctcgc cttgatggta ttgcacgcgt aacggtgtgc ctaccagctg gaaggctttg
     2521 cggaaaatgc cttcgagata acgagtatag ctcgctttga cgccatcgat atggttacca
     2581 tgtaccacca caattggcgg attggtgccg ccctgatgtg catagcgcag tttggggcgg
     2641 atgcctttgc taattggggg ctggtgttgt gtagtcgctt cacctaggac acgtgtcagt
     2701 tgtggggttg ccaatttggc catggctgct ttgaacgcca cgtctaccga gcccagtaat
     2761 tcattcaaac cttttttacg caaagccgag atgtagtgga acttggcaaa atccaggaat
     2821 tgcagtttgc ggtcaatctc tcgtttgatc cactcgcgtt catcatcctg aagcccgtcc
     2881 catttgttga tggcgaccac taatgcgcgg ccagcgtcga caatgtaggc ggcgacatgc
     2941 gcatcttgtt ccgtaatacc ttctttggca tccaccacca gaatcgccac attagcatct
     3001 tcaatggctt gcatggtctt gatgacggag aacttttcta ccgcttcaaa aactttaccg
     3061 cgcttgcgta caccggcggt atcaataatc gtgtattgct tgccattttt ttcaagggca
     3121 atttcaatcg agtcacgtgt ggtgccaggc tggtcaaacg caatcacgcg gtcttcacca
     3181 agtaaggcat tcaccaaagt cgattttcca acattgggtc gacctacgat ggcgattctt
     3241 ggattgtctg gattgactgg ttcagagttt tctatttctt tgtcgctgat ggtttccagt
     3301 gccagctcaa tcaggtcgcg tacaccttca ccatgagcag atgaaatgga cagcggttcg
     3361 cccataccta gttcgtgaaa gtctgcactg acaatggctt tgcgcatgcc ttctgtcttg
     3421 tttacggcca gcaggacagg gcgttgtgct ttacgcaggg tctgcgcaat ggtgaattcc
     3481 tgaggagtta atcctgcgcg ggcatcgacg agaaagataa taacatcggc ttcgtcaatg
     3541 gctaacaagg tttgtttggc catttccttc aggatgccgg tgtcagcagt tggttcaaag
     3601 ccaccggtat caatgaccag gtatggctgg cttgctccca ggccgcggcc gtagtggcgg
     3661 tcgcgggtca agccgggcaa atcggcaacc agcgcatcac gcgttttggt caggcgatta
     3721 aataatgtgg atttgccgac atttggccgg ccaacaagta cgatggtagg taacattatt
     3781 tgtatgtgac tgcgtataga tggccgtcac gcgattgcgc aaagaactga ttaggttttg
     3841 aatcagcaat cagtgacatg atgggtttgg aacccaactg tgttcggcca acaaaactgc
     3901 catcatcctg gctgagtaaa tgaatatagc cttccaggtc acccacagct acgacattac
     3961 tcagggggag tggcacggtc aggttgcggt atcccagatt gccctgacgc cagaaggttt
     4021 taccattatt ggcgtcaagt gaatagactg agcctatggt atggcttaaa tagactttgt
     4081 tgttttcagc agtcaggcca acataactgg agatatcacg gttccacacg atttggccac
     4141 gctgacggtc aaccgcagcc actttgccct ggtaagccac aacatagacc aatgggccat
     4201 caacgaatgg caggctggta atgtcggcga tacgctcaat ttctgtcacg cctttaggct
     4261 gggcaacggt ggtctcccag accagtttgc cattgtcggc gcggattgcg ataagcttgc
     4321 cgccaggaaa gccggcatag actgcgccgc catccacgac aattcctgcc tgtgtcttga
     4381 gcgacagtga aggggtcgcg cgctcatagg accatttgcg gcggccatct gctgcgtcaa
     4441 tcccgtaaat atggtgatcg ctcgtacgta caatgaccgt gccatcataa tagcggggct
     4501 gaccctgaat ttcgctactc aggatggcgc tccaaagttt cttgccgttc acgttcaggg
     4561 cgtacaaatg acctttgctg gtgccgacca ataccaggcc gccacccaaa cctgcaccgc
     4621 tgctaatggg ttcgccaagt tcggtttccc aaactgtttt gcccgtttca gcagatagtt
     4681 gcctgatgct gccttcggca ttcacggcat aaatcaggtt gtctgccagc actggtctgt
     4741 aagtaaaacg tccagcttcg cccacatcag tcgaccagag cacacgcatt tcatatgaag
     4801 acttgatgtc ctcgagctct actggcgggt tggcaggctc tgccccaaaa atactttcac
     4861 tgaggttggt tttaacatca ttgaaagccg tgcatgcaga tagcccgctg cctaataagc
     4921 ataaggcggc gagcgtgaca tgtttgagtg attgtggctg attgcgcagg gcagagaagc
     4981 taaacttcat atatcgttac cctagggcat ccagcttttg ctgggtcagg taaaacagtt
     5041 tgccttcagg atccagacct aacaaggctt tgttataagc ctctttggct tctttttcct
//
LOCUS       DATMSK010000018         6014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_212278,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000018 DATMSK010000000
VERSION     DATMSK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212278"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..417
                     /locus_tag="VNZ84_00540"
     CDS             277..417
                     /locus_tag="VNZ84_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65464.1"
                     /translation="MCFYGPLSAAPSSAIGVGDVGQRLSEFRGGLRSVQAARASFVGR
                     PR"
     gene            complement(556..1446)
                     /gene="ftsX"
                     /locus_tag="VNZ84_00545"
     CDS             complement(556..1446)
                     /gene="ftsX"
                     /locus_tag="VNZ84_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWU65465.1"
                     /translation="MKNWLNQHALAIDLVLQRLKSHWLNTITICMVIGITVTLPGLLF
                     VALDNLQDMSRSIKQDAQISAFLKPAVNANIVQRLINDVEALPQVKEVRFVTKEEAIK
                     QLGQQFLQKDLLADLPQNPLPDALFITLTDTQPASVKPVQDLLQKRGEVDELVIDSVW
                     IERLHSMLVLGKRIALIFASLLGIAMITVISNTVRMQVLTHEAEIEVSRLIGATHSFI
                     RRPFLYMGGAYGLGGGLIAVALLFLIVWNLRQPVQLLANNYQSNFQLVFHLSNVGGLM
                     LVATTAIGWIAAFIALTQKR"
     gene            complement(1468..2145)
                     /gene="ftsE"
                     /locus_tag="VNZ84_00550"
     CDS             complement(1468..2145)
                     /gene="ftsE"
                     /locus_tag="VNZ84_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWU65466.1"
                     /translation="MIAFNNVSMRYPNSAHLFRQVSFQIDKGDFVLVTGHSGVGKSTL
                     LKLMAGIEHPSSGHIMINEQDISKLHPNAVPYLRRRFGLVFQDHKLLYDRNSFDNVAL
                     PLSIQGITGEEAARRVRAALDKVGLLNKEKAMPITLSGGEQQRLAIARAVVSRPSIVI
                     ADEPTGGLDQAASLEIMQLLKAFNDVGVTVVIAMHDVAQVSTIAYRTLHIDQQTISTM
                     PAQTAML"
     gene            complement(2160..3146)
                     /gene="ftsY"
                     /locus_tag="VNZ84_00555"
     CDS             complement(2160..3146)
                     /gene="ftsY"
                     /locus_tag="VNZ84_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWU65467.1"
                     /translation="MFNPFAKKSDPVTPPATASASLSWTERLKNGLAKTRQQLGGQLS
                     ALFGGGKINEEIYEELETILLTSDVGINATQYLLEQLRNRVKKQDLQDTVALKQALKE
                     TLLELLGPLESPLDVSNHQPYVMMMVGVNGAGKTTTIGKLAKYLQAQGHSVLIAAGDT
                     FRAAAREQLQVWGERNDVHVIASESGDPAAVIYDAVNSAIAKKIDVVIADTAGRLPTQ
                     LHLMDEIKKVQRVIDKALPGAPHEILLVLDANTGQNAVNQLKAFHEALGVTGLALTKL
                     DGTAKGGVIAAIAYLSQQSSSKTPVRFVGVGESIDDLRPFNANDFIDALIDD"
     gene            3349..4731
                     /locus_tag="VNZ84_00560"
     CDS             3349..4731
                     /locus_tag="VNZ84_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWU65468.1"
                     /translation="MKKTVSLAGLLALCMSTHAVVAQADIEQKTLKNGLKVIVQEDHR
                     APVVVSQVWYRAGSLDEVNGKTGVAHVLEHMMFKGTKKVPVGMFSRQIAAAGGKENAF
                     TSKDYTCYFQQLEKSRLPLSFQLESDRMANLQITDAEFAKEIEVVKEERRWRTEDKPQ
                     SRVNEQFESSVYRAHPYGRPVVGFMNDLENMTADDAREWYRNWYAPNNATVVVVGDVK
                     AKEVFALAEKTFGQLKAKPLPVRKPQVEPAQIGERRAMVKAPAKLPYFVMGFHTPVLK
                     NGVAYSEQDWEPYALEVLSSILSGNDASRLNQKLVREQAIALEIDTGYDSTNRGQTST
                     FEVAASPSEGVSQEDLIKAIWAQIEEVKNHGVTAAELHRIKAAVIAADVYKRDSMFYQ
                     AMQIGQLETMHYPLSLLQNNAARVQAVTSEQVQAVAKKYLVPDQMTLVSLDPQPIDPN
                     QKPAGRPHQH"
     gene            4781..>6014
                     /locus_tag="VNZ84_00565"
     CDS             4781..>6014
                     /locus_tag="VNZ84_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWU65469.1"
                     /translation="MNVFVLFRTVLLALALQAYAMNVFAALNIQHWTTAEGSEVYFVE
                     NHSLPMMDVSINFRAGSAYDTLETSGAAAVTHHLMSLGAGGLDEETLTNQFADIGAVL
                     GGSFDADRASFKVRMLTSEQQAALDTFMLVLHKPDFPQNVLVREQARIVAALQEAETE
                     PDSIAQKAFMQSVYGMHPYSMDQNGEISTVQSLTVAQLREFYQTYYTAKSAVIAMMGD
                     LTTEQAKQIAERLSAGLPQGPAVAAIPPVKELNVPSIKKFPHPAVQAHILIGQPGNMR
                     GDPDFFPLYVGNYILGGGGFVSRLTEEVREKRGLAYSVYSYFMPMSQLGPFQIGLQTK
                     KEQADEALKLVMETVQVFVEKGVTEQELAAAKDNLIGGFPLRIDSNSKILEYLNMIGF
                     YKLPLDYLDNFNQHVGQVT"
BASE COUNT         1472 a         1469 c         1628 g         1445 t
ORIGIN      
        1 cgatctcgtc cagcgaaact cgccctagcg gcttgcaaag aacgcaagcc accgcggaac
       61 tcggacagcc gctggccgaa tactcccacg ctgattgcgc tactcggcgc ggcagatagg
      121 ggcccgttaa aacccaccac gatgaaacgt tcgttgtgta tgttaaattc agggcgctag
      181 ataacttttc actttaaagc cgaaattact aaccatttgg ttttttcatg gtgatcgagc
      241 ggggcgcaat gcaaagaatc gcactaaagt gaaagcgtgt gtttttatgg gcccctatct
      301 gccgcgccga gtagcgcaat tggcgtggga gatgtcggcc agcggctgtc cgagttccgc
      361 ggtggcttgc gtagtgtgca agccgctagg gcgagtttcg ttggccgccc acgttgatga
      421 gcaacgcagg aaataagcgg aagctagggt gtcttgttct ttgccttctt tcttatggac
      481 aagcataaga aagaacggtt gcctagaggc gaaaagaagc gttactcaca tagcaacaga
      541 agaaaacgga gattgctatc gcttctgcgt caatgcaata aacgccgcaa tccacccgat
      601 ggccgtggtt gccaccagca ttagtccacc gacattagac aagtgaaata ccagctggaa
      661 attactttga tagttattag ccaacaactg caccggttgt cttaaattcc atacaatcaa
      721 aaacagcaag gccactgcaa tcaggccgcc acccagccca taagcgccac ccatgtaaag
      781 aaaaggacga cggataaagc tgtgcgttgc acctatgagt cgactgactt caatttcggc
      841 ttcatgcgtc agcacctgca tgcgaacggt gttgctgatg actgtaatca tggcaatccc
      901 cagcaggctg gcaaaaatca acgcaatgcg tttgccaaga accagcatgg agtgcaggcg
      961 ctcaatccag acactgtcga tcaccagctc gtcaacttcg ccgcgttttt gcaataggtc
     1021 ctgcaccggt ttgactgagg ctggctgggt atccgtcagt gtgataaata gcgcgtccgg
     1081 cagcgggttt tgcggcaagt ctgccagcag gtctttttgc aagaactgtt gacccaattg
     1141 cttgatggct tcttccttgg tgacgaagcg gacttctttt acctgaggta aagcttcaac
     1201 atcgttaatc aggcgctgaa caatattcgc attgaccgct ggtttcagga aggcgctgat
     1261 ttgcgcatcc tgtttgatcg aacgggacat atcctgaagg ttatccagcg cgacaaagag
     1321 caggccgggc agggtgacgg tgatgccgat caccatgcag atggtaatcg tattcagcca
     1381 gtgcgatttc aggcgttgca agaccaggtc tatggccagt gcgtgttggt taagccagtt
     1441 tttcatgcag ctcttctcct tgtctggcta taacatggcc gtctgtgccg gcatggtgct
     1501 gatggtttgc tggtcaatat gcaaggtgcg gtaagcaatg gtgctgacct gggcgacgtc
     1561 atgcatggca atgacgaccg tgacgccgac atcgttaaac gctttgagca gttgcatgat
     1621 ttcgagcgag gcagcctggt caagcccacc ggttggctca tctgcaatca caatcgatgg
     1681 gcggctgacc acggctcgtg caatcgcaag ccgttgttgt tcgccgccag acaaagtgat
     1741 aggcatggct ttttctttat taagcaggcc gactttatct aatgctgcac gcacacggcg
     1801 ggcggcttct tcaccagtaa tgccttgtat gctgagtggc agggcgacat tatcaaagct
     1861 gttgcggtca taaagtagtt tatgatcctg gaatactagg ccaaaacggc ggcgcagata
     1921 tggcacagcg ttgggatgca gcttgctaat gtcctgctca ttgatcatga tgtggccact
     1981 ggaggggtgt tctatgcctg ccataagttt aagcagtgta cttttgccaa cccctgagtg
     2041 gccggttacc agcacaaaat cgcctttatc gatctgaaaa ctgacctggc gaaacaaatg
     2101 cgcgctattg gggtagcgca tgctgacgtt gttaaaagca atcataggcg actctatggt
     2161 tagtcatcaa ttaaagcgtc aataaaatca ttggcattaa acggtcgtaa atcatcaatg
     2221 ctttcgccga cgccaacaaa gcgtaccggc gtcttgctcg acgattgctg gctcaagtag
     2281 gcaatcgcgg cgatgacgcc gcctttggcc gtgccgtcca gcttggttaa ggccaggcct
     2341 gtgacgccca gtgcttcgtg gaatgctttg agctggttga cggcattctg gccggtattg
     2401 gcatccagta ccagcaggat ttcatgcggt gcgcctggca aagctttatc aatcacacgt
     2461 tgcaccttct tgatttcgtc catcaggtgc aactgggttg gcaggcggcc agccgtatcg
     2521 gcaattacga catcaatttt tttggcgatg gctgaattga ccgcatcata aatgacggcg
     2581 gcgggatcac cactctcgga ggcgatgaca tgcacatcat tacgctcacc ccatacctgc
     2641 aattgttcac gtgcggcagc gcggaaagta tcgcctgccg caatcagcac gctgtggcct
     2701 tgtgcctgca ggtatttggc cagcttgcct atggtggtgg ttttgccagc accgttcacg
     2761 ccgaccatca tcatgacata aggttgatga ttgctgacat ccaatggtga ttcgagtggt
     2821 cccagcaact ccagcaaagt ttccttcaat gcctgtttga gggcaacggt gtcctgcagg
     2881 tcctgctttt tgacccggtt gcgcagttgc tccagcagat actgcgtcgc gtttatgccg
     2941 acatctgaag tgagcaggat ggtttccagc tcttcataaa tctcttcatt aattttgcca
     3001 ccaccgaaca gggcactcaa ttgccctcct aactgttggc gtgttttggc cagaccgttt
     3061 ttcagacgtt cggtccagct caggctggca gacgctgtag cagggggtgt gactgggtcg
     3121 gattttttag caaaaggatt aaacatgatg gctgaaaact cggtaatatg gttattttaa
     3181 gccgaatcga cgattcccgc cacggcaagc ttgtggtgcc agcgtgcgga tacggttgat
     3241 tcaagcagtg gcgaacttag tcattgatgc atcagtcaca cacaaggttt caaatccaat
     3301 tcgttcattg atccctaata attgatctta agataaaaaa tgtatcccat gaaaaaaacg
     3361 gtttccctgg ctgggttact cgcactttgt atgagcacac atgcggtggt ggcgcaggcc
     3421 gatattgaac aaaaaacctt aaaaaacggt ttgaaagtca tcgtacagga agaccaccgt
     3481 gcacccgtcg tcgtgtccca ggtctggtac cgtgctggct cactggatga ggtgaatggc
     3541 aaaaccggcg ttgcacacgt gcttgaacat atgatgttca aagggaccaa aaaggtcccg
     3601 gtcggtatgt tttcgagaca aattgccgct gccggcggta aggaaaacgc cttcacgagc
     3661 aaagattaca cgtgctattt tcagcagctg gaaaaatcca ggttgcctct gtctttccag
     3721 ctggaatcag accgtatggc caatctgcaa attacagatg ccgagtttgc caaggaaatt
     3781 gaagtggtga aagaggagcg ccgctggcgt accgaggata agccacaatc gcgcgtcaat
     3841 gaacagtttg agtcttcggt ttaccgggca catccctatg gccgtccggt ggtgggtttt
     3901 atgaatgacc ttgaaaacat gaccgcagac gatgcgcgcg aatggtaccg caactggtat
     3961 gcgcctaata atgcaacagt ggtggtagtg ggcgacgtca aggcgaagga ggtatttgcc
     4021 ctggctgaaa agacatttgg tcaattgaaa gccaagcctt tgcccgtacg taaaccacag
     4081 gtggaaccag cacaaattgg cgaacgccgt gccatggtca aggcgcctgc caagttgcct
     4141 tattttgtga tgggctttca tacaccagta ttgaaaaatg gtgtggcata tagcgagcag
     4201 gattgggagc cttatgcgct ggaagtctta tccagcatcc tgagtggcaa tgacgcttcc
     4261 cgcctgaacc agaaactggt acgtgagcag gcaattgcgc ttgaaattga tactggctat
     4321 gacagcacta accgcggcca gacttcaact tttgaggtcg cggcttctcc gagtgagggt
     4381 gtttcacaag aggatttgat caaggcgatc tgggcacaga ttgaggaagt caagaatcac
     4441 ggggtaaccg cagcagagtt gcaccgtatc aaggcggcag tgattgccgc cgatgtctat
     4501 aaacgtgatt ctatgtttta ccaggcgatg cagatcggac aattggaaac catgcattac
     4561 ccgctgtcgc tgttgcagaa taatgctgcc agggtgcaag ccgtcacctc ggagcaggtg
     4621 caggccgttg ccaaaaaata cctggtacca gaccagatga cgctggtcag cctggatccg
     4681 caaccgatag atcccaatca aaaacctgcg ggtcggccgc atcaacacta aatcatctct
     4741 aattgcagtt acctggtagg caggtgaatc aaggaaccac atgaacgtat ttgttttatt
     4801 tagaaccgtt ttgctggcac tggcattaca ggcgtatgcc atgaacgtgt ttgcagccct
     4861 gaatatccag cattggacca cagcagaggg cagtgaagtg tatttcgtag aaaatcactc
     4921 attgcccatg atggatgtca gcattaattt ccgcgctggc agtgcgtatg acacgcttga
     4981 aaccagcggt gctgcggcgg tgacgcatca cttgatgtcg ctcggcgcag gtgggctgga
     5041 cgaagaaaca ttgactaacc agtttgctga tattggcgcg gtgttaggcg gcagttttga
     5101 tgccgatcgc gccagcttca aagtgcgcat gctgaccagt gaacaacagg ccgcgctgga
     5161 tacctttatg ctggtgttac acaagccgga ttttccacaa aatgtgctgg tacgcgagca
     5221 ggcaaggatc gtggccgcat tgcaggaagc tgaaaccgag ccggacagca ttgcgcagaa
     5281 agcctttatg cagtcggtat atggcatgca tccgtacagc atggaccaga acggtgaaat
     5341 ctctacggtt cagtcgctga cggttgccca actgcgcgag ttttaccaaa cgtattacac
     5401 ggccaagtct gctgtcattg ccatgatggg ggatttgacg acagagcaag ccaaacagat
     5461 tgccgaacgc ttaagtgccg ggttgccgca agggcctgca gtagccgcta ttccaccggt
     5521 caaagaactt aatgtgccca gtatcaagaa attcccgcat cccgccgtgc aagcccatat
     5581 cctgatcggc cagcccggca atatgcgtgg cgacccggat ttctttcctt tgtacgtggg
     5641 taattatatt ttgggtggcg gtggatttgt ctcgcgcctg actgaagagg tacgtgaaaa
     5701 acgtggcctg gcttacagtg tttatagcta cttcatgccc atgagccagc tgggcccatt
     5761 ccagattggt ttgcaaacca agaaagagca ggcggatgag gcactgaagc tggtaatgga
     5821 aacggtacag gtctttgtcg aaaaaggggt gaccgagcaa gagcttgctg ccgcaaaaga
     5881 taacttgata gggggctttc cgctgcgtat agacagcaac agcaagattc ttgagtacct
     5941 caacatgatc ggcttttaca agctgccttt ggattacctg gataatttta accagcatgt
     6001 ggggcaggtg actg
//
LOCUS       DATMSK010000019        14187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_236210,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000019 DATMSK010000000
VERSION     DATMSK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 14187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14187
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236210"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VNZ84_00570"
     CDS             <1..159
                     /locus_tag="VNZ84_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU65470.1"
                     /translation="AWHTHMVNHGVRITETPKTHRDGSRGFYCLDSVGHLLEFIYHPP
                     IVRHVQGL"
     gene            complement(224..541)
                     /locus_tag="VNZ84_00575"
     CDS             complement(224..541)
                     /locus_tag="VNZ84_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HWU65471.1"
                     /translation="MQKFSDNLLTLDSADHVKRIELYDENNQPAGVIENKPGTQGSIK
                     VYHHLFKMFGEINTDAAVEGLALYAEHTEDAEDHPGKHPNIDRLLNVLDKEQTLAIKV
                     IIE"
     gene            complement(567..1211)
                     /gene="coq7"
                     /locus_tag="VNZ84_00580"
     CDS             complement(567..1211)
                     /gene="coq7"
                     /locus_tag="VNZ84_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HWU65472.1"
                     /translation="MLTLPPVTLDKAIAVMDMGLRTVFSSAMASRPNPAAGTINEVPL
                     TEEERKHAAALMRINHVGEVCAQALYSGQALVSRDPANVEALKQASREEVDHLAWCEQ
                     RITELGGRKSLLNPLWYGASFGLGVTVGLLGDKWNLGFLAETEHQVSAHLHSHLHELP
                     AQDHASRAIVAQMKTDEEQHAHTAEQLGGAALPGPVKLAMRLASKVMTRTAYHL"
     gene            complement(1278..2705)
                     /locus_tag="VNZ84_00585"
     CDS             complement(1278..2705)
                     /locus_tag="VNZ84_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWU65473.1"
                     /translation="MRSKFEKFGLLGAGVFVGILVSINISAWADRTSVSQLPIDELRV
                     FAEVFAKVKSDYVEPVEDKKLINEALSGMLQGLDPHSTFMDADAYKELQAGTQGEFGG
                     LGIEVAMEDGLVKVVTPIEDSPAYKAGLKSGDLIMKLDDTQVRGLTLNDAVKRMRGTP
                     DTKITLTVLRKGEDKPLTFTLTRAIIKAQSVKNKWIEQDYGYVRITQFQERTGEDLAK
                     ALKTLLAENKKPLKGLVLDLRNNPGGLLDAAVGVSAAFLPKDDLVVYTEGRVVDSKMR
                     LNATPMDYARRGKSDYLKDLPEEFKTVPIVVLINAGSASASEIVAGALQDHKRATIMG
                     IQSFGKGSVQTILPMNNGSAIKLTTARYFTPNGRSIQAKGIVPDVIVDDGSDPSLSLH
                     EADLKRHLSNPTDAKNNADSKAVDEEKSRAAAEKLKEEKHAETRGPIEPASKDDFQFT
                     QAMNLLKGLPVQKAPEPAKVSTAKP"
     gene            2879..3175
                     /locus_tag="VNZ84_00590"
     CDS             2879..3175
                     /locus_tag="VNZ84_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HWU65474.1"
                     /translation="MQKPIVEIEFCTQCRWLLRAAWLAQELLTTFDTDLKSVSLVPGT
                     GGILEVRCDGRVIFSRKEMGRFPEAKELKQFIRDLVDPERSLGHSDSPVKGGGE"
     gene            complement(3193..3747)
                     /locus_tag="VNZ84_00595"
     CDS             complement(3193..3747)
                     /locus_tag="VNZ84_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HWU65475.1"
                     /translation="MNKSFIRLAALLVSVLLCQCKSVAAPSDDKRWQNAYSYSQEQVM
                     ENQSGVAGSWSEKVEEIMISALSLTGVDYKYGGRSPETGFDCSGFVQYVFKHAAQISL
                     PPSARTISEMGDAVKREDLQPGDLVFFNTLRSAFSHVGIYVGNNQFIHAPRAGAKVRV
                     ESMDERYWRTRYNGAKRLEVEASK"
     gene            3930..5882
                     /locus_tag="VNZ84_00600"
     CDS             3930..5882
                     /locus_tag="VNZ84_00600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HWU65476.1"
                     /translation="MSLQQQVSSAFSENGCLAQAMPAFRSRPQQQEMAQAIAEAIEQQ
                     QQLVAEAGTGTGKTYAYLVPALLSGGKVIISTGTKTLQDQLYQRDLPAVREALKVPVS
                     VAMLKGRSNYICHYHLQRASQEGRFQSREDAQYIPILQSFAENSQSGDKAELVEVPEQ
                     ATVWQQVTSTRDNCVGQDCQFYKQCFVMEARKQALAADVVVVNHHLFFADVMLRDEGV
                     AELLPSANTVVFDEAHQLPEVAGLFFGEDISTSQLMDLARDSQMAYLSLAKDCVALSE
                     AVPPLEKACRDFRLVFQFEGSRMPAQKAQTLKNFDDTYQRMLESLSTLTTVLETQAGR
                     DPLLEKCWQRGEALFALLDAWLKGNHANLVRWVEVFSQSVQLHATPLSVADGFGKQLN
                     AQPRAWIFTSATLAVKSDFSHYQGQMGLLDAQTRHWQSPFDYGEQALLYVPTGMPEPN
                     SSAYAAAVAAVSLPVLKASRGRAFVLCTSLRAMREVHALLRQAFEENGLDYPLLMQGE
                     SNRTELLDRFRTHGNAVLVGSQSFWEGVDVRGEALSCVIIDKLPFAPPDDPVLAARID
                     KMNAEGKNAFMEYQLPYAVITLKQGAGRLIRDEQDLGVLMICDPRLVDKPYGRRIWQS
                     LPPFRRTRVQQEVEDFFTLINGAAQP"
     gene            5879..6280
                     /gene="arfB"
                     /locus_tag="VNZ84_00605"
     CDS             5879..6280
                     /gene="arfB"
                     /locus_tag="VNZ84_00605"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWU65477.1"
                     /translation="MIDIPTHEYVLSAIRAQGAGGQNVNKVSSAIHLRFDILASSLSE
                     AIKHRLLHSHDQRITAEGVLVIKAQQYRTQEANKRDAIRRLHAIVEAASHVHAVRYAT
                     RPTLASKKRRVAGKIQRGLVKRGRGRVVEEG"
     gene            complement(6300..7781)
                     /gene="lnt"
                     /locus_tag="VNZ84_00610"
     CDS             complement(6300..7781)
                     /gene="lnt"
                     /locus_tag="VNZ84_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWU65478.1"
                     /translation="MWLSQTSLWQRTILALLLGTLTVFGFAPFGLFWLPWLTLSGLLW
                     LWHRAATAGQVFKLGLAFGLGFYVFGIYWIYISLHTFGGMPWWFAGFSTFCLCAFMAL
                     FPALAGYLAKKMRHLLWAAPILWALSDWIRSWIFTGFPWLTLGYSQAPESPLAGFLPL
                     IGVYGVSALVMLLATCLLVIITQPKPLPAALVTTALLVGGSLLTQVPWTHQTGEPVDV
                     ALLQGNIGQTIKWSPEHAEHTLKQYLDMVRQTQAHLIVLPETALPVLVEQLAPAYLDA
                     LKQHAQSQHGDLLVGVVESKQGEYFNSAISLGTAPSQSYSKSHLVPFGEYIPLKGVFG
                     WIYRDWLHMPLSDLSRGTSKQPLLIAGQKVGVNICYEDVFGEEIAQQLPEAELLVNIS
                     NDAWYGQSFAAEQHMQFSQVRAMETGRMVLRSTNTGATAIIDKNGQVLQHAPHDEAAI
                     LVGKAQSYQGTTPYVWWGNWAFLVLSIGSLVLIQLKKARPDMF"
     gene            complement(7787..8638)
                     /locus_tag="VNZ84_00615"
     CDS             complement(7787..8638)
                     /locus_tag="VNZ84_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWU65479.1"
                     /translation="MAKLIICADDYAQSPAIDDAILMLIERGVLTATSCMTLSPLWPD
                     SAKALTPQLRRLADIGVHLDFTQFSQTIRYRHPLLVLRSLSGTLHKQHVVANIQQQLD
                     AFEQALDTPPDYVDGHLHIHQLPIIREALISVLQQRYGHLPVAERPWLRISSPPVGSG
                     LKARIIHWLGAEKLKRLAHAAGFRVSPALLGVYDFEGNAATYQAHWMHWVKQLKQLTF
                     TPTLDMDLPPVLMCHPARPTPKVDPDDPIAIARMVEWQVMQSADFSAWLAMTDIQPVK
                     GTMKGLS"
     gene            complement(8648..9022)
                     /locus_tag="VNZ84_00620"
     CDS             complement(8648..9022)
                     /locus_tag="VNZ84_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWU65480.1"
                     /translation="MKASVLGFVSVGALAAAVHYVAALLAHDYGLQPATANWIGFLCA
                     FPVSYLGHRHWSFRGTQASHVAAFFKFLAVALLGFLGNQVLLWLALTYTLLPFWLALG
                     IVMVIVAVSTWLLSRFWAFNHA"
     gene            complement(9034..10026)
                     /locus_tag="VNZ84_00625"
     CDS             complement(9034..10026)
                     /locus_tag="VNZ84_00625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWU65481.1"
                     /translation="MTHTDDDRQTAQPHITVMVPAYNEEKNIARTLQNISSVLQKMSP
                     YWDMVVVDDGSRDQTSQVVQSLVESCRVTLVRFSRNFGKENAISAGLSYAKGDLVICM
                     DADGQHASELMLTMLEKWREGYDMVYAVRQDREQESSFKLWGSRLFYRMMSASTHVEI
                     PRDAGDFRLMNRKVVDALCALPERNRFMKGIYAWVGYKSIGIPYTPLPRLHGESAYSK
                     LKLIALAWTGITGFSVLPLRLASLTGATLALLAFAYGTVVVIDALFLHESVPGWLTVV
                     ASMMFFAGVQLLFIGILGEYLARVYDEVKGRPPYIVAEVVQPAMTAKTPDTSAS"
     gene            complement(10072..11700)
                     /locus_tag="VNZ84_00630"
     CDS             complement(10072..11700)
                     /locus_tag="VNZ84_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65482.1"
                     /translation="MTLATSHPQSPAIYRWLMRLFWLGLFIWVALTFRQHGISNDEYV
                     QHTYGQMLLDWYQSGFKDQDAFHYRNLYLYGGLFDIIAAAIDPYIPIMIWDIRHLLSA
                     LFGLLGFYGVAKFADLLGGQRLSLLAMTLLLLTVSWSGTMFTHTKDIPFATCMLWALY
                     ATALVVRDMENVSGRAIVLLGFAVGGALGLRVGGAFAVIYLILLVVGRVLLDHHQERR
                     PILSRLLRMVVVLLPAAVLAFVFMAMCWPWSVIEADNLLEAAKSFSHFDFNMQTIANG
                     VVYNISEVPRSYLFQYLAVKLPEAALLGLFALPVLCLCCRQALCIADRPTRMVMYTLL
                     VGTLFPLVFVMDDRPALYNGVRHFTFLLPLLAILAAWCITAVWQALANSHLIKPSFYQ
                     AIWAGVMTGLMLVTLVDMIQLHPYQYVRLNRLAGDQTQAQFKWEGDYWSSALRAATEQ
                     LTEMNLPKRDQPYWIGACVDTPQIEPYLDGRFSVTRRWEEADFYLATTNMHCHEVMKG
                     KWIGGVQRQGMWLAVIKDRRQLQGADRLGTPAKN"
     gene            complement(11825..12529)
                     /gene="phoB"
                     /locus_tag="VNZ84_00635"
     CDS             complement(11825..12529)
                     /gene="phoB"
                     /locus_tag="VNZ84_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HWU65483.1"
                     /translation="MAAANILVVEDEPAIQELLALNIKQAGHHAIRATSVEHAQMLLR
                     ETMPDLILLDWMLPGMSGIEFARRLKSDSLTKEVPIIMLTARGDEFDKVRGLEVGADD
                     YVTKPFSPRELNARIKAVLRRRAPQMTDDPIEIAGLHLDPTTHRVTGHNVHIPLGPTE
                     FKLLHFFMTNPERVHSRTQLLDKVWGDRVFVEDRTVDVHIRRLRNALVASKHEEMIQT
                     VRGSGYRFSVHTDPGN"
     gene            12698..>14187
                     /locus_tag="VNZ84_00640"
     CDS             12698..>14187
                     /locus_tag="VNZ84_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HWU65484.1"
                     /translation="MLINLSKEHITAGNHFNRWLVPPAALAVHLSIGMAYGFSVFWKP
                     LGNALTGPDGKPVEACAAGASTFGDKLAGTAKALFATDCNWTQFDLGWMYTLFFVLLG
                     CSAALWGGWLERAGPRKAGLVAMLCWCGGLLLSALGVYQHQLWMMWLGSGVIGGIGLG
                     LGYISPVSTLIKWFPDRRGMATGLAIMGFGGGAMIGSPLATKLMSIFATPASPGVWQT
                     FVVLALIYAVFMTLGSLGYRVPAANWKPADWTPPDVQHNRLVASRHVHLNNAHKTPQF
                     WLLWLILCMNVSAAIGIIGAAAPMLQETFGGVLIGQPSLGFAEIKADEALTAAAAAVG
                     AGFVGLISLFNIFGRIGWATSSDKLGRRNTYFIFFVLGIMMYVCGTYAANHHQLALFL
                     LVFCIIASMYGGGFATIPAYLADLFGTQFVGAIHGRLLTAWSTAGILGPVIVNYMHDV
                     RLEAQVPYADIYAPIFMTLAGLLGVGFIANLLVRPVADKYFMSDSE"
BASE COUNT         3358 a         3578 c         3772 g         3479 t
ORIGIN      
        1 gcctggcata cgcacatggt gaatcatggc gtacgcatta cagaaacacc taaaacacat
       61 cgcgatggct cacgcggatt ttattgcctg gacagtgtcg ggcatttgct ggagtttatt
      121 taccacccgc cgattgtgcg gcatgtgcaa gggctgtgag tattgcaagc atgaaaaaag
      181 cctccattaa gtggaggctt ttttgtgagc ttaattcggg cgattattcg ataatgactt
      241 tgattgccag tgtttgctct ttgtccagca cattgagcaa acggtcaatg ttgggatgtt
      301 tgcccgggtg gtcttcggca tcttcggtat gttcggcata cagggctaat ccctctactg
      361 ccgcatcagt attgatttca ccaaacatct tgaacagatg atggtatacc ttgatggagc
      421 cctgggtgcc cggtttgttc tcaatcacgc cggctggctg attgttttca tcatacagtt
      481 caatgcgttt tacgtggtcc gcactgtcca gggtcagaag attgtcacta aatttttgca
      541 tggttgactc taggttacta caaatcttac aagtgatagg cggtacgcgt catcactttt
      601 gacgccaatc gcatggccaa ctttaccggg cctggcagcg cagcgccacc cagttgctct
      661 gcagtgtggg cgtgttgttc ttcatcagtt ttcatctgcg caacaatcgc ccgagaggcg
      721 tgatcctggg ccggcagttc gtgtaagtga ctatgtaaat gcgcagatac ctggtgttca
      781 gtttcagcca ggaagccgag gttccatttg tcgccgagca ggcctacagt gacgcctaag
      841 ccaaagctgg caccgtacca caatgggttg agcaagcttt tgcggccgcc cagttctgta
      901 atacgttgtt cgcaccatgc caggtggtcc acttcttcgc gagaggcttg tttgagtgcc
      961 tcgacgttgg ccggatcacg cgaaaccagc gcttggccgc tatacaaggc ctgtgcacag
     1021 acttccccca catggttgat gcgcatgagt gctgcagcgt gtttgcgttc ttcttccgtc
     1081 aaaggcactt cattgatagt accggccgca ggattggggc gactggccat tgccgaactg
     1141 aacactgtac gcaagcccat gtccatcacc gcaatggctt tgtccaatgt tactggcggt
     1201 agtgtcaaca tcatcgctcc ctctttattt acccggctta tactctggat tagcagtgca
     1261 ggccggggtt catcacgtta tggttttgcg gtgctcactt tggcaggttc tggcgctttc
     1321 tggacaggta ggcccttgag caagttcatc gcctgggtga actggaagtc atccttgctg
     1381 gcaggctcaa tcgggccacg ggtttcagca tgtttttctt ccttgagttt ttcagcggct
     1441 gcacgggatt tttcttcatc tacagctttg ctgtcagcat tgtttttggc atccgtcgga
     1501 ttggacaaat gacgtttcaa atctgcttcg tgcaagctga ggcttggatc agaaccatca
     1561 tcaacgatca catcggggac gatacctttg gcctggatag agcggccatt cggtgtaaag
     1621 taacgcgcag tggtcagctt gattgcgctg ccgttgttca taggcaaaat ggtttgcaca
     1681 gagcctttac caaaactctg gatacccatg atcgtggcgc gcttgtgatc ttgcagtgca
     1741 cccgccacaa tttctgatgc agaagcagag cctgcattga tcaataccac gataggcacg
     1801 gttttgaact cttcaggcaa gtctttcaag tagtctgatt tgccgcggcg tgcataatcc
     1861 ataggcgtcg cattcagtcg catttttgag tcaactacac gaccctctgt gtacaccacc
     1921 aggtcgtctt ttggcaagaa ggctgctgaa acgcccactg cggcatccag taagccgcca
     1981 ggattgttac gcaggtctaa cactaaacct ttcaacggtt ttttgttttc agccagcaag
     2041 gtttttaagg ctttggccaa gtcttcgccc gtacgttcct ggaactgggt gatacgcaca
     2101 taaccatagt cctgttcaat ccatttgttt ttcacgcttt gcgctttgat aatggcacgt
     2161 gtcagtgtga aggtcagtgg cttgtcttca cctttacgca gcacggtcaa tgtgattttc
     2221 gtatctggtg taccgcgcat acgtttgaca gcgtcattca gcgtgaggcc gcgcacctgg
     2281 gtgtcatcca gtttcataat caggtcacct gatttaaggc ctgccttgta agccgggctg
     2341 tcttcaatcg gggtgaccac tttcaccagg ccatcttcca tggccacttc tatgcctaac
     2401 ccaccaaatt caccctgggt gccggcttgc aattccttgt aggcgtcggc atccataaag
     2461 gtagagtgcg gatccaggcc ttgtagcatg cctgataacg cttcattgat aagttttttg
     2521 tcttctacgg gttctacgta gtcagatttt actttggcga acacttcggc aaatacacgc
     2581 agctcgtcta tgggtaactg agataccgag gtccggtctg cccaggcaga gatgttgatg
     2641 ctaactagaa tgcctacaaa tacgcctgca cctagcaagc cgaacttttc aaatttggaa
     2701 cgcatacaat gccttgtgtg atcaattaag cgaagaatat tactgtatta agagtcaagt
     2761 tgcatcaatg gatgtcagcc tgtttgcaga gagtaggtag aatgccttta agttcatttt
     2821 ccgctatttc tgtaaaattg actatacttt tcacgcaaat aaatgtttag agagaaacat
     2881 gcaaaagcct atcgtagaaa ttgaattttg tacacagtgc cgttggttgt tgcgagctgc
     2941 ctggttggcg caagagctac ttaccacgtt tgatactgat ttaaagtccg ttagcctggt
     3001 gcctggcaca ggtgggatac ttgaggtgcg ctgtgatggc cgtgtgattt tttcacgtaa
     3061 ggagatgggg cggtttcctg aggcgaagga gttgaaacag ttcatccgtg atctggtaga
     3121 tccggaaaga tcactggggc acagtgatag ccctgtcaaa gggggcggtg aataaagcga
     3181 gtgggctggt tgttatttgg acgcttcaac ttctaggcgt ttggcaccat tgtagcgcgt
     3241 gcgccagtag cgttcgtcca tgctctcaac acggactttt gcgcctgctc tgggcgcgtg
     3301 tatgaattgg ttgttgccga cgtaaatgcc aacgtgtgaa aacgcagatc gcaaggtatt
     3361 aaagaacacc agatcaccgg gttgcaaatc ttcgcgtttg acggcatccc ccatttcact
     3421 gatggtccgc gctgaaggtg gcagggaaat ctgggcggca tgtttgaaga cgtactggac
     3481 aaaaccactg caatcaaaac cggtctcggg gctgcggcca ccgtatttat agtccacacc
     3541 ggtcaggctt agtgcgctga tcatgatttc ttctactttc tcagaccagc taccggcaac
     3601 gcctgactga ttttccatga cctgttcttg cgaatagcta tacgcgtttt gccagcgttt
     3661 atcatcgctg ggcgcggcaa cacttttgca ttgacatagc aacacgctga ccagtaaagc
     3721 agccagccga atgaacgatt tattcatcac aatcaaaggc ttggaaatcg gggttggatt
     3781 gtaaaaggat agatacggcc taaaccttgc catactttaa tggcagatgc aagtatttgg
     3841 ccgtttttgc ggtgtttgtg atcatcgtca agcgtgccgg tttgttgaaa aagcctgcga
     3901 tttgctcgtg ctcgtgggat aattaaaata tgtctttaca gcaacaagtc agttccgctt
     3961 tttcagaaaa tgggtgtttg gcgcaagcca tgcctgcttt tcgtagccgt ccgcagcagc
     4021 aggaaatggc gcaagccatt gccgaagcca ttgaacagca acagcaactg gtggccgagg
     4081 ctggtaccgg cactggcaaa acctatgctt acctggtgcc tgcgctgtta tcgggcggta
     4141 aggtgattat ttcaacaggc accaagacgc tgcaagacca gctgtaccaa cgcgatttgc
     4201 cggccgtgcg cgaggcgctc aaagtacctg tgtctgtggc catgctcaag ggccgttcaa
     4261 attatatctg ccactatcat ttgcagcgtg ccagccagga aggccgtttc cagtcgcgag
     4321 aagatgcgca atacattcct attctgcaaa gttttgccga gaatagccaa agcggtgaca
     4381 aggcagaact ggtcgaagtg ccagaacaag ccactgtctg gcagcaggtg acctcgacgc
     4441 gcgacaactg cgtgggacag gattgccaat tttacaagca gtgttttgtg atggaggcgc
     4501 gcaaacaagc cctggcggcg gatgtggttg tggtcaacca tcacctgttt tttgccgatg
     4561 tcatgctgcg tgacgagggt gtggccgaat tgctacccag cgccaataca gtcgtatttg
     4621 acgaggcgca tcagctcccg gaagtggctg gtctgttttt cggtgaagat atttctactt
     4681 cgcaactcat ggatctggca cgggacagcc aaatggctta cctgagtctg gcgaaagatt
     4741 gcgttgccct cagcgaggcc gtgcccccgc tggaaaaagc ttgccgcgat ttcaggctgg
     4801 tattccagtt tgaaggctca cggatgccgg cgcaaaaagc gcaaacgctt aaaaattttg
     4861 atgacactta tcagcgcatg ctggaaagct taagcacatt gacgactgtc ctagaaacgc
     4921 aggctggccg tgacccattg ctggaaaaat gctggcaacg cggagaagcg ctatttgcat
     4981 tactggatgc ctggttgaaa ggcaatcatg ccaatctggt acgctgggta gaggtgttta
     5041 gccaaagtgt acagttgcat gctaccccgc ttagcgtggc cgatgggttc ggcaagcagt
     5101 tgaatgcgca gccgcgtgcc tggattttta cttcggccac cttggcggtg aaaagcgact
     5161 tcagccatta ccaagggcag atgggcttgc tggatgcgca aaccaggcac tggcaaagcc
     5221 cgttcgatta tggcgagcaa gccttgctgt atgtgccaac gggcatgccc gagcccaata
     5281 gcagtgctta tgccgcagca gtggctgccg tgagtctgcc ggtccttaaa gccagtcgtg
     5341 gccgtgcatt tgtgctttgc acctcgctca gggcaatgcg cgaagtgcat gccctgctcc
     5401 ggcaggcgtt tgaagagaat ggcctcgatt atcccttgct tatgcaaggc gaaagtaacc
     5461 gtaccgaact attggaccgt ttccgtacgc atggcaatgc cgtactggtc ggttcacaaa
     5521 gtttctggga aggcgtggat gtgcgtggtg aggcattgtc atgcgtcatt atcgacaagt
     5581 tgccatttgc gccgcctgat gatccggtgc tggccgcacg catcgacaag atgaatgccg
     5641 aaggcaagaa tgcttttatg gagtatcagc tgccatacgc ggtgattacc cttaagcagg
     5701 gtgccgggcg cttgattcgc gacgagcagg atttaggggt gttgatgatt tgtgacccgc
     5761 gcctggtcga taaaccttac ggccgtcgca tctggcaaag tttgcctcct ttcaggcgta
     5821 cgcgcgtgca gcaagaggtt gaggattttt ttaccttgat taatggcgcc gctcagccat
     5881 gatagatatt cctacccacg agtatgtact gagtgccatc cgggcacagg gggcgggtgg
     5941 gcaaaacgtc aataaagtgt cttctgccat tcatttacgg tttgatattc tagcttccag
     6001 cctgagtgag gcgatcaagc accgtttgtt gcatagtcat gaccagcgga ttacggctga
     6061 aggtgtgttg gtgatcaagg cgcagcaata ccggacacaa gaagccaata aacgggatgc
     6121 gattaggcgg ttgcatgcga ttgttgaggc tgcgagccat gtgcatgcag tcagatacgc
     6181 gacaagaccg acattggcgt cgaaaaaacg gcgtgtcgca ggcaaaatac aacgcggatt
     6241 agtgaagcgg gggcggggca gggtggtcga agaggggtga ccagacgctg ctctagccat
     6301 cagaacatat caggtcgcgc cttcttcagc tgaatcagca ccagactgcc tatacttagc
     6361 accagaaacg cccagtttcc ccaccaaaca taaggcgttg taccctggta actttgtgct
     6421 ttgccgacca gtattgcggc ttcatcatgc ggtgcgtgtt ggagtacctg accgttcttg
     6481 tcaatgatgg cggttgcccc tgtgtttgta ctgcgcaaca ccatgcgccc ggtttccatg
     6541 gcacgtacct ggctaaactg catatgttgc tctgccgcaa acgattgtcc ataccaggca
     6601 tcattgctga tattgaccag tagttcggcc tcaggcagtt gctgggcgat ttcttcacca
     6661 aatacgtctt cgtaacaaat gttcacgcct actttctgac cggcaatcag tagcggttgc
     6721 ttggaagtgc cacgtgacaa gtcacttagt ggcatgtgta accagtcacg gtaaatccag
     6781 ccgaatacac ctttgagcgg aatatattcg ccaaagggga ccaggtggct tttggaatag
     6841 gattgcgaag gtgctgtccc caggctgatg gcgctgttga agtactctcc ctgtttggac
     6901 tccaccacac ccacgagtaa atcgccatgc tggctttggg catgttgctt cagcgcatcc
     6961 aagtaggcgg gagcgagttg ttcaacgagg actggcagtg ctgtttcagg caagacgatc
     7021 aaatgagcct gtgtttgccg caccatgtcg aggtattgtt tcagggtgtg ttctgcatgc
     7081 tcgggtgacc atttgatggt ctgcccaatg ttcccttgta gcaaggccac atccactggc
     7141 tcacctgttt ggtgtgtcca tgggacttgt gtcaacaggc tgccgccgac cagtagcgcg
     7201 gtcgtgacca atgcggctgg caatggtttt ggttgggtga tgatcacaag caaacacgtt
     7261 gcaagcagca tcaccaatgc cgaaacacca tagaccccaa tcagaggtaa aaagcctgcg
     7321 agtggactct ccggagcctg gctgtaaccg agggtcagcc aggggaatcc ggtaaaaatc
     7381 cagctacgta tccaatccga caaggcccat aagatggggg ctgcccaaag caaatgccgc
     7441 attttcttgg ccaggtagcc tgcaagtgcc gggaacagtg ccataaatgc acataggcaa
     7501 aaagtagaga accctgcaaa ccaccatggc atgccgccaa aagtatgcag gctgatataa
     7561 atccagtaga taccaaacac ataaaagccc aggccaaatg ccagtcccag tttaaagacc
     7621 tggccagcag tggctgcccg gtgccataac cagagcaggc cggacagcgt cagccagggt
     7681 agccagaaca gtccaaacgg tgcaaacccg aacacagtga gtgtgcctaa taacagcgcc
     7741 agtattgtcc gttgccaaag agatgtttgg ctcaaccaca ctaaattcaa cttaagcctt
     7801 tcattgtgcc ttttactggc tgaatatctg tcatggctaa ccaggcagaa aagtctgccg
     7861 attgcatcac ttgccactcc accattctgg cgatagcaat cggatcgtca ggatcgacct
     7921 tgggggttgg ccgtgccgga tgacacatca gcacaggcgg caaatccatg tcgagtgttg
     7981 gtgtgaatgt caattgcttg agttgcttca cccagtgcat ccaatgcgcc tggtaagtcg
     8041 cggcatttcc ctcaaaatca tataccccca gtaacgctgg tgagacgcgg aagcctgcgg
     8101 catgcgcaag gcgtttcaat ttttctgccc ctaaccaatg gatgatacgg gctttcaggc
     8161 cactgcctac aggcgggctg gaaatgcgta gccatggccg ttcagcaacg ggtaaatgac
     8221 catagcgttg ctgcagcaca ctgatcagcg cctcgcggat gattggcagc tgatgtatgt
     8281 gtaaatgtcc atcgacgtaa tccggtggtg tgtctagcgc ctgttcaaat gcgtctagtt
     8341 gctgctgaat attcgcgacc acgtgctgtt tgtgaagcgt accagacagg ctgcgtaata
     8401 ccagtaatgg atggcgatag cgtatggtct gggagaactg cgtgaaatcc aggtgcacac
     8461 cgatatccgc caggcgacgc agttgcggtg ttaacgcttt cgcactgtca ggccacaacg
     8521 ggcttaaggt catgcaggag gtggccgtca gtacacctcg ttctatgagc atcaggatgg
     8581 cgtcatcaat cgcaggtgat tgagcatagt catcggcaca gataatcaac ttggccatga
     8641 atatccttta cgcatgatta aacgcccaga atcggctaag tagccaggtg ctcaccgcaa
     8701 cgataaccat cacgatgcct aatgccagcc agaaaggtaa gagagtgtag gttagcgcca
     8761 gccagagcaa aacctgatta cccaaaaagc cgagtaaagc cactgccaga aacttgaaaa
     8821 aagcggccac gtggctggcc tgtgtcccgc gaaatgacca atgacgatgt ccgagataac
     8881 tgaccgggaa ggcacataaa aatcctatcc agttcgcagt tgcaggttgg aggccgtagt
     8941 catgcgccag taaggccgcg acataatgca cggctgcggc caacgcacct acactgacaa
     9001 atcccagaac tgatgctttc atgcagtcat caattaggat gcgctggtgt caggcgtctt
     9061 cgccgtcatt gccggttgaa cgacttctgc cacgatgtaa ggggggcgtc ctttgacttc
     9121 gtcgtagacg cgcgccaggt actcgcccaa aatgccgata aacaacagtt gcacccccgc
     9181 aaaaaacatc atgctggcga ccaccgttag ccagccaggg acagactcat gcaggaatag
     9241 tgcatcaatc acgaccaccg taccataagc aaacgccagc aaggccaagg tggctccggt
     9301 caaactggct aaacgtaaag gtagcaccga gaacccggta attcctgtcc aggccaatgc
     9361 aatcaatttg agttttgaat aggcactctc gccatgcagg cgtggcagtg gcgtgtatgg
     9421 aatgccgatc gacttgtagc cgacccaggc atagatgcct ttcatgaagc ggttacgttc
     9481 gggcagggcg cacaaggcat ccaccacctt gcggttcatc aggcggaagt ccccggcatc
     9541 gcgcgggatt tcgacatgtg tactcgcact catcatacgg tagaacagtc gcgagcccca
     9601 cagtttgaac gaagactcct gctcacgatc ctggcggacg gcgtagacca tgtcgtagcc
     9661 ttcacgccac ttttccagca tggtcagcat aagctcagag gcatgctgac catcagcatc
     9721 catgcagatg accaagtcac ctttggcata actcaagccg gcactgatgg cattttcctt
     9781 gccaaagtta cgcgaaaacc tgaccagggt gactctgcat gactcgacta atgattgaac
     9841 tacctgggaa gtctggtcac gactgccatc atcaacgacc accatgtccc agtatggaga
     9901 cattttctgc aatacactgg agatattttg caaagtgcgc gcgatatttt tttcttcatt
     9961 gtacgcaggt accatgaccg tgatatgcgg ctgggcggtt tggcgatcgt cgtctgtatg
    10021 agtcatggca tcacgtggag gatatactgc agctattgta acggattggc actagttttt
    10081 ggcaggcgtg cccaggcgat cagcaccttg taattgacga cggtctttaa tcaccgccaa
    10141 ccacattccc tggcgttgca cgccaccgat ccatttgcct ttcatgacct catggcaatg
    10201 catattggtt gtggccaggt aaaagtcagc ttcttcccaa cgccgtgtga cactgaaacg
    10261 gccatccagg taaggctcta tctgaggggt gtcaacacac gcgcctatcc agtaaggttg
    10321 gtcccgctta ggtaagttca tttctgttaa ctgttctgtc gcagcacgta gtgcactgga
    10381 ccaataatca ccttcccact tgaattgcgc ctgggtttga tctccggcca ggcgatttaa
    10441 cctgacatat tgatagggat gtagctggat catatcgacc aatgtcacca gcattaaccc
    10501 cgtcatgaca cccgcccaga tggcctggta gaaagacggt ttgatcaggt ggctgttcgc
    10561 cagggcctgc caaacggcag tgatgcacca ggcggcaagg atcgccagga gcggcaataa
    10621 aaaagtaaag tggcgtacgc cattataaag cgcaggccgg tcatccatca cgaataccaa
    10681 ggggaacagg gtgccgacta ataaggtgta catgaccatg cgcgtcggcc ggtcagcgat
    10741 acagagtgcc tgccggcaac acaggcaaag cacaggcaaa gcgaataagc caagcaaggc
    10801 ggcttctggc agtttgacag caaggtattg gaacaagtag ctgcgaggaa cttcactgat
    10861 gttgtagacc acgccattcg caatggtctg catattaaaa tcaaaatggg agaacgattt
    10921 ggcagcctct agcaggttgt ctgcctcaat cacgctccac ggccagcaca tggccataaa
    10981 cacaaatgcg aggacggctg caggcagcaa aaccaccacc atgcgcagta aacgtgacag
    11041 gataggccta cgctcctgat ggtggtcgag caatacgcgg ccaactacca gcaagatcag
    11101 gtaaatgaca gcaaaggcac caccgacacg caatcccagc gcgccaccga cggcaaaacc
    11161 aagcaggaca atcgctcggc cactgacatt ctccatatcg cggaccacca aggcggtagc
    11221 atacagtgcc cataacatac atgtcgcaaa tgggatgtcc ttggtgtgtg taaacatggt
    11281 ccctgaccat gacacagtga ggagtaacag tgtcattgcg agtagcgaca ggcgctggcc
    11341 cccgagcaag tccgcaaact tggcaacacc ataaaaaccc aacaggccaa aaagcgccga
    11401 gagtaaatga cggatatccc agatcataat gggaatatag gggtctatcg ccgccgcaat
    11461 aatgtcgaac agaccaccgt ataaataaag attccggtaa tggaatgcat cctggtcctt
    11521 aaagcccgac tggtaccagt cgagcagcat ctggccgtag gtgtgctgca catattcatc
    11581 attgctgatg ccatgctgac ggaaagtgag tgctacccaa ataaacagtc ccagccaaaa
    11641 caggcgcatc aaccagcgat aaatagctgg cgattgagga tgggaagtgg caagtgtcat
    11701 agggggcatt cattgtgaaa aatcaacacg cgcaagcgta tcacgacacc ctgccggaga
    11761 atagagcgtg agcagcctca cgctcaaaaa atagtgagtg aattgaactg gtggaacgac
    11821 ttgattagtt accggggtcc gtatgcacgg aaaaacgata accggaaccg cgcaccgttt
    11881 gaatcatttc ttcatgtttg gatgccacca aagcattacg caaacgacgg atatgtacgt
    11941 ccacagtacg gtcttccaca aacacgcggt cgccccatac tttgtccagc agttgagtac
    12001 ggctgtgcac acgctcaggg ttggtcataa agaaatgcag taatttaaat tcggttggtc
    12061 ccagcgggat atgcacattg tgaccagtca cgcgatgtgt ggtcggatcc aggtgcaaac
    12121 cggcgatttc gatcgggtca tcggtcattt gcggtgcgcg tctgcgcagg accgccttga
    12181 tacgtgcatt gagttcgcgt gggctgaatg gcttggtcac atagtcatct gcacctactt
    12241 ccaaaccacg caccttgtca aactcatcac cgcgcgcggt cagcatgatg atgggtactt
    12301 ccttggtcag actgtcagat ttcagtctgc gcgcaaattc aatgccactc atgccaggca
    12361 acatccagtc cagcaagatc aggtccggca tggtttcacg taacaacatt tgtgcatgtt
    12421 caacgctggt ggcacggatg gcatgatgcc ccgcctgttt gatattcaac gccagcaact
    12481 cttgaatcgc cggttcgtct tctaccacca aaatattcgc tgcagccatg acactccctt
    12541 caagcaatat tgagatcaaa gtattgattt aatgctcgcc agtttatgaa tgaattatga
    12601 cagtttaatg acaaactcac ataaatttaa atttgctttt attgcctttt gggctaattt
    12661 gtaacaagat gttacggcaa aaaataaagg ggttttgatc ttgattaatc tttccaaaga
    12721 acatatcaca gctggcaatc atttcaatcg ctggctggta ccaccagctg cgctggctgt
    12781 acatctttcc attggcatgg cttatgggtt cagcgtattc tggaagccgc taggcaatgc
    12841 attgactggt ccagacggta agcctgtaga ggcttgcgcc gccggggcaa gtaccttcgg
    12901 tgacaaactc gcagggacag caaaagcact cttcgctact gactgtaact ggactcagtt
    12961 tgacctcggt tggatgtata ccctgttctt cgtactgttg gggtgttcgg ctgcgctgtg
    13021 gggagggtgg ctggagcggg ctggcccacg caaagccggc ttggtagcga tgttgtgctg
    13081 gtgtggtgga ttactgctct cagccctggg tgtttaccag caccagttat ggatgatgtg
    13141 gctgggtagt ggcgtcattg gcggtatagg gcttggactg ggctatattt cgccggtctc
    13201 taccctgatc aaatggtttc ctgaccgccg tggtatggcg accggcctcg cgattatggg
    13261 ctttggtggt ggggctatga taggttcgcc actggccacc aagctcatga gcatctttgc
    13321 gacgcctgca tcacctggtg tctggcagac ttttgtcgta ttggcactga tttatgccgt
    13381 attcatgact ttaggctcac tgggctatcg tgtgccggca gccaactgga agccagcaga
    13441 ctggacgccg ccagatgtac aacataaccg tctggtcgcg tcccgccatg tccacctgaa
    13501 caatgcacat aaaacaccac aattctggct gctgtggtta attctatgca tgaacgtgtc
    13561 agcggccatt ggcattattg gtgccgcagc gcccatgttg caggaaacct ttggtggggt
    13621 gctgattggt caaccatcac tcggatttgc cgaaattaaa gctgacgaag cgttgacggc
    13681 agccgcggcg gcggttggtg ctggctttgt aggcctgatc tcgctgttta acatttttgg
    13741 ccgcattggc tgggccactt cttccgataa actgggccgc aggaatactt atttcatctt
    13801 ctttgtgctt ggcatcatga tgtatgtctg tggtacttac gcagccaatc accatcagtt
    13861 ggccttgttc ctgctggtgt tttgcattat tgcctccatg tatggcggcg gttttgctac
    13921 tattcctgca taccttgctg acctgttcgg tacgcagttt gtaggtgcca ttcatggccg
    13981 gttactgacc gcctggtcca ctgccggtat tcttggccca gtgattgtta actatatgca
    14041 tgacgtcagg ctcgaggcgc aggtgcccta tgcagatata tatgccccta ttttcatgac
    14101 attggcaggt ttgctggggg ttggtttcat cgccaacttg ttggtaagac ccgttgcaga
    14161 caaatatttt atgtctgaca gtgagat
//
LOCUS       DATMSK010000020         4924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_259673,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000020 DATMSK010000000
VERSION     DATMSK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259673"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VNZ84_00645"
     CDS             complement(<1..751)
                     /locus_tag="VNZ84_00645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWU65485.1"
                     /translation="MLIDTHCHLDASEFSADRDQIWQEAQAEGVRALVIPAVMQSTFQ
                     PIMDWCARHPQTGFALGWHPLFVDQAPDDALQQMHEAVAAVLAGPKAQQLLAIGEIGL
                     DFYVTRENEAKQIALFEGQLQIAKQFDLPVILHVRAAVDSVLKYLRKHHIHQGIAHAF
                     NGSLQQATIFAELGFKLGFGGAMTWPRALKIRKLAAEVPLEHVVLETDSPDIPPVWLG
                     HQGRNSPGQLKRIAEEMALIRGLEVSQLIEIT"
     gene            complement(755..1846)
                     /gene="ribD"
                     /locus_tag="VNZ84_00650"
     CDS             complement(755..1846)
                     /gene="ribD"
                     /locus_tag="VNZ84_00650"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWU65486.1"
                     /translation="MSQSTQDIEWMTHALRLAARGLYTTTPNPRVGCVIVNDGQLVGE
                     GAHLKAGEPHAEVHALRAAGVQARGATAYVTLEPCSHFGRTPPCADALVEAGVSRVVV
                     AMQDPNPLVSGNGIARLRAQGITVVVGIGEAQARLLNPGFIQRMTAQRPYVRLKVAAS
                     LDGRTALANGTSQWITSGDARKDVHHWRAQSCAIVTGIGSILKDDSSLTVREVATTRQ
                     PLRVIVDSQLRIPLNARVLQDGHALIAYAHADVDKLEALQAMGVRTLHAPDVRGQVDL
                     SLLMKNLVTVPCNEILVEAGATLNGAFLQSGFVDELLLYYAPKLMGHTARGMFALPDM
                     TQMADVRELRVLEVRQFGQDLRLQAKLQP"
     gene            2061..4205
                     /locus_tag="VNZ84_00655"
     CDS             2061..4205
                     /locus_tag="VNZ84_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65487.1"
                     /translation="MIYIADPNPWLALYAQPLHTRVTPPTADLQSFLSAYGRSQQLTC
                     ALASATQDLEADLVEALAGLPESLLNILSKSVLGIFFADGLGASGLTDMVALGDGSNN
                     IGFIIVLDSKAFTHASANAWASWKENSAFIPDQQVQIDVRIADQHSDHRVQAMRFLLL
                     HEFGHVLATMTDLCPKEWTLPLPKLAGHFSKFSWQTSGSKSIRPEQNFPHREKISFYR
                     QPALHASQALDLYRDLARTAFPTLYASMDVQEDFAECFATYMHTQVLGQPYTVQVKVK
                     DVAVFNSDDFWASPRASDKQRYMHHLLQSLEHNNAGYTFQGLAPFLRMNLNGSDLRAH
                     GQSLLVQANQDQENAILWMNLATVFLCLKMRDMGLAIQEQALSLQRLYIRPANCRPAK
                     FRLLMIMTPGDLSENTPIDCLLENSPVELLYYYATTEQPLPSPLPAHDAVMVGLSDSQ
                     ANRALLLAVQASLEGHTKPVINPPHLLPNVNRDQLSTLLQGVAGLDMPRTHRLSRSQV
                     VTRLSMGNHSTHNNPDALPGFPFIIRPVGSQAGNDLARVTSTSELAEYLRTVPSTDFF
                     VSRFVDYSSQDGQFRKYRIAMLHGQPFICHMAISSHWMVHYVNAGMYDSADKRAEEGL
                     FMQDFSSFATRHQAALAAIYQRLQLDYLCIDCAETAAGDLLVFEIDHIMAVHAMDSAN
                     LFPFKATHIAKLQQAFEQYLYALKTQPLLESA"
     gene            4202..>4924
                     /gene="serC"
                     /locus_tag="VNZ84_00660"
     CDS             4202..>4924
                     /gene="serC"
                     /locus_tag="VNZ84_00660"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HWU65488.1"
                     /translation="MMKLNANQLNFSGGPGALPESVLQQLQAAMLCVPEVGLSILGIS
                     HRSQWFAQVVQELEENIRHLMGLPDNTRVLMMQGGATQQFSMLPMNLLRHGESADYIH
                     SGYWSGKAIHEAEKSGQLNLAWSGKADQFSRLPSDAELQLSKDAAYFHYISNETVEGL
                     QFHRVPGRDDVPRICDMSSDLLSAPGEFNRYAMIYAHAQKNIGPAGVTLVILRDDFMQ
                     TLPKDLPGFINYHAQADAHSIYN"
BASE COUNT         1200 a         1425 c         1175 g         1124 t
ORIGIN      
        1 ctgtgatttc aatgagttgc gaaacttcca agccacgaat taaggccatt tcctcggcta
       61 ttctttttaa ttgacctgga ctattcctgc cctggtgacc cagccaaacg gggggaatat
      121 ccggggaatc cgtctccaaa accacatgct ctaatggcac ctctgccgcc aatttgcgaa
      181 ttttgagtgc gcgtggccaa gtcattgcac cgccaaagcc caatttaaaa ccgagctctg
      241 caaatattgt tgcctgctgc aggctgccat taaatgcatg cgcaatcccc tgatgtatat
      301 gatgcttacg caagtatttg agcacactgt ccactgctgc ccgcacatgc aaaatgaccg
      361 gcaggtcaaa ctgcttggca atctgtaact gcccttcaaa tagcgcaatc tgcttggctt
      421 cattctcgcg cgtcacataa aaatccaggc cgatctcccc tatcgccagc aactgctgcg
      481 cctttggccc agctaacacc gcagcgactg cctcatgcat ttgctgcaaa gcgtcatcag
      541 gcgcctgatc cacaaacagg ggatgccacc ccagcgcaaa gccggtttgc gggtggcgtg
      601 cgcaccaatc catgatgggt tgaaaagtgg attgcatcac agccggaatc accagcgcac
      661 gcaccccttc ggcttgcgcc tcttgccaga tctggtcacg atcggcagaa aattcgctgg
      721 catctagatg gcaatgtgta tcaatcagca taagtcaggg ttgcaattta gcctggagac
      781 gcaggtcctg gccaaattgt cgcacttcaa gcacacgcag ctcacgcacg tctgccatct
      841 gtgtcatgtc tggcaaggca aacatgccgc gtgcagtatg gcccatcagt ttgggagcgt
      901 aatacagcaa tagttcatcg acaaagccac tttgcagaaa agccccattg agcgtcgctc
      961 cagcctcaac cagtatttcg ttgcaaggca cggtgaccag gttcttcatc aatagagaca
     1021 ggtcgacctg tccgcgaaca tcaggtgcat gcagtgtacg cacacccata gcttgcaagg
     1081 cctccagctt atcgacatcc gcatgcgcat aagctatcaa ggcatgaccg tcctgcaata
     1141 ccctggcatt cagcggaatg cgtaactgac tatccactat cacgcgcaaa ggttgcctgg
     1201 ttgtcgccac ctccctcacc gtcaatgagg aatcatcctt aaggatactg ccaatgccgg
     1261 tcacaatggc gcagctttgc gcacgccagt gatgtacatc tttacgcgca tctccagacg
     1321 taatccactg actcgtacca ttagccaacg ctgtacgacc atccaggctg gcagcaacct
     1381 tcagtcgcac ataggggcgc tgcgctgtca tacgctggat aaacccagga ttcagtaacc
     1441 gggcttgtgc ttcaccaatc ccgacgacca ccgtgatgcc ctgcgcacgt aaccttgcaa
     1501 tgccgtttcc ggaaaccaag ggattgggat cctgcatggc caccactacc cggcttaccc
     1561 cggcttctac cagagcatcc gcgcatggcg gcgtacgccc aaaatgacta catggctcca
     1621 gtgtcacata ggctgtcgcc ccgcgcgcct gcacgcctgc tgcacgcaag gcatgaactt
     1681 ctgcatgcgg ctcccctgcc ttcagatgcg ccccttcgcc gaccagctgg ccatcgttaa
     1741 caatcacgca acccacgcgc ggattagggg tagtggtata caggccacgc gctgccagac
     1801 gcaaggcatg cgtcatccat tcaatatctt gtgtactttg tgacatagac tgccatgata
     1861 caaaaaaaca tggcctcgcg gttttttgtg atgtcctcaa gcagcaagtt cgccataacc
     1921 caccggcatg acctaagaca ttcgactgac gcatttctta agcaaaaaac ggcgattaaa
     1981 cttggctaat tgcagaatac aatggttttt gccagttttt ttgcggcaac tcctcgcgcg
     2041 tgcgggcgct acactagggt atgatttata tcgccgaccc caatccctgg ctggccctgt
     2101 atgcacaacc attgcataca cgtgtcactc cacccactgc agacttacag tcgtttctat
     2161 cggcatacgg ccgatcgcaa cagttaacct gcgccctcgc ctcagcgacc caggacttag
     2221 aggctgacct cgtagaagcg ctggctggct tgccagaaag cctgttgaat atcctcagca
     2281 agtctgtttt gggaattttc tttgcagatg gcctgggagc atccggctta actgatatgg
     2341 tcgcgttggg agatggtagc aacaatatcg gattcatcat cgtgctggat agcaaggcgt
     2401 tcacgcatgc atccgccaat gcatgggcaa gctggaaaga gaattctgcc tttatccctg
     2461 accagcaggt gcaaatcgat gtgcgcattg cggatcaaca tagcgatcat cgcgttcaag
     2521 ccatgcgatt cctgttacta cacgaatttg gccatgtcct ggccaccatg acagatttat
     2581 gccccaagga gtggacgctt cccctgccca agctggcagg acactttagc aaattcagtt
     2641 ggcaaacgag cggaagcaaa tctatccgcc cggaacagaa ctttccccat cgagaaaaaa
     2701 tctcgtttta ccgccaaccg gcactgcatg ccagccaggc actggatctt taccgcgact
     2761 tagccagaac cgctttcccc accctgtatg ccagcatgga tgtacaggaa gactttgccg
     2821 agtgcttcgc cacctatatg catacacaag tgctggggca gccctatacc gtgcaggtca
     2881 aggtaaagga tgttgcagta tttaacagcg atgatttctg ggcctcgcct cgtgccagcg
     2941 acaagcaacg ctacatgcat cacttgctgc agtcattgga acacaacaac gcaggataca
     3001 ctttccaggg gctggcacct ttcctgcgca tgaacctgaa tggttcagac ttgcgtgcac
     3061 atgggcaatc gttgctggta caggccaacc aggatcagga aaatgccatc ctgtggatga
     3121 acctggccac ggtattttta tgcctcaaga tgcgagacat gggattagcc atccaggaac
     3181 aggcgctctc actacaacgc ctctacattc gcccagccaa ttgtcggcct gcaaagtttc
     3241 gcctgctcat gatcatgacg cccggtgatc tttctgaaaa tacaccgatt gattgcttat
     3301 tggaaaacag cccggtagag ctgctctatt actacgcgac gacagagcaa cctctgccat
     3361 cacctctacc ggcccacgat gccgtgatgg tcgggctatc agatagccag gccaaccgtg
     3421 cgctgttgct ggcggttcaa gccagcctgg agggacatac aaaaccggtt atcaacccac
     3481 cacacttgct acccaacgta aaccgcgatc aattgagtac gctgttacag ggagtcgccg
     3541 ggctggatat gcctcgtact caccgccttt cgcgcagcca ggtagtgaca cgtttaagca
     3601 tgggcaacca cagtacccac aacaatccgg atgcattacc aggctttcca ttcattatcc
     3661 gccctgtcgg ttctcaagcc ggtaatgacc tggcaagagt caccagcaca tctgaactgg
     3721 cagagtattt aaggaccgtc ccaagtaccg atttctttgt atcgcgcttt gttgactaca
     3781 gcagccagga tggccagttc cgcaaatata gaatcgccat gctgcacggc caaccattta
     3841 tttgtcatat ggccatttct agccactgga tggtgcatta cgttaatgcg ggcatgtacg
     3901 actctgccga taaacgcgcc gaagaaggtc tgttcatgca ggacttctcc agtttcgcca
     3961 cgcgccatca agcggctttg gccgcaatct accagcgttt gcaacttgac tatctgtgca
     4021 tagactgcgc tgaaacagcc gctggtgact tactggtatt tgagatcgac catatcatgg
     4081 cggtgcatgc gatggattcg gccaacctgt tcccgttcaa ggcgacgcac attgccaaat
     4141 tgcaacaggc ttttgaacag tatttatatg cattaaagac gcaaccttta ttggagagcg
     4201 catgatgaaa ctcaatgcca atcaattgaa tttttctggt gggccagggg cattaccaga
     4261 gagtgttctg caacaactcc aggcggccat gctctgtgtg cccgaagtcg gattgtcgat
     4321 tttaggcatt agccatcgct cgcaatggtt tgcccaagtg gtacaggaac tcgaagagaa
     4381 catccgccac ctgatgggtt tacctgacaa tacacgcgtg ctcatgatgc aaggcggcgc
     4441 cacgcagcaa ttctccatgc tgcccatgaa cctgctacga catggcgaat ctgcagacta
     4501 catccacagc ggctactgga gtggcaaagc catccacgaa gcagaaaagt ctggccagct
     4561 caacctggca tggagtggca aggctgacca gtttagccgc ctgcccagcg atgctgagct
     4621 gcaactctca aaagatgctg cctattttca ctatatctca aatgaaacgg tagaaggttt
     4681 gcagtttcac cgcgtgccag gcagggacga cgtcccacgt atctgcgata tgtcatcaga
     4741 cctattatcg gcacctggtg aattcaaccg ctatgccatg atctacgcac acgcgcaaaa
     4801 aaacattggc ccggcaggcg tgacgttggt cattctgcgt gatgatttta tgcagacact
     4861 gcccaaggac ttacctggct ttatcaacta ccatgctcag gcggatgccc attcaattta
     4921 caac
//
LOCUS       DATMSK010000021         7542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_1693, whole
            genome shotgun sequence.
ACCESSION   DATMSK010000021 DATMSK010000000
VERSION     DATMSK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2036
                     /locus_tag="VNZ84_00665"
     CDS             <1..2036
                     /locus_tag="VNZ84_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU65489.1"
                     /translation="YRLDTGNQILRAFTLYQGQFHRAVKKGNLQTLGLVERGMATISQ
                     AEITALETKWKGSHLQHGVYPKQVIWALALTLLVAATLSLLVYMLRVAVKRKTAEIES
                     KREELELERLRLTDIIDATRAGTWEWNIQTGECQFNHRWAEMLGYTLNALAPFNAEMV
                     KALTHPEDWKTAMALVERHLKGEMDFYECDIRMRHQDGHWVWITDRGRLISRTITGEP
                     WIMRGTHIDITDKKQADAVIWQQANYDSLTQLPNRHYFHQQIKEALAAAIDTQQRLTL
                     LLLDLDRFKEVNDSLGHKRGDELLKEVGQRIRQCVLPPATIARLGGDEFAIILPEALQ
                     ADLQALCNKLMQDISAAYILGGERIFVTVSIGISQYPEDAVNAEEMIQHADQAMYDAK
                     GRGRNSLRFFSLAIRDALSQRVSLARDLRTALENGELRDYYQPIVNLRSGKIVKAEAL
                     MRWQHPERGLISPALFIPVAEETGDIIAMGDWIFNQAAQHAAQWESLVSDDAFAISVN
                     KSPVQFVDKMGAKNDWVHALRSMGVAGKRIVVEITEGLLLNPDPVVEERLLQYRDMGI
                     QVAIDDFGTGYSSLSYLSKFDIDYLKIDQSFTRNLNAGNESYVLCEAIIVMAHKLGLK
                     VIAEGVETALQRDLLIAIHCDYAQGFFYSPPIPAEAFEQMLVQQSREEAAAAK"
     gene            complement(2233..3696)
                     /locus_tag="VNZ84_00670"
     CDS             complement(2233..3696)
                     /locus_tag="VNZ84_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWU65490.1"
                     /translation="MTFKHFFKLALLPLTISQAFAAQAAEQSSTTAQESPSLVSRFMN
                     DDNPFRINFDLGDHPSYFQVYGLVDIGLTHINHSLPENYELANNFYPYAGAKKTSRVT
                     SRTNWVNGGWQGSRLGFKGEVAKLQAWDHDFKFIYQFEAGFNTLDMRLHDAAQTLADN
                     SGTNANSSVSANSSMNGELFTRQAWAGIDGGSWGKLTYGTQYNPFFEITVTYDPNGKA
                     DSFSPLGESGTVGGGGGVSENSRMKNSLKYANVYELESHDKINYAVINQFGNSAGTAH
                     GNGYTTQLGYESSAFGVQLAYDRFFDSVKSGTAAAGNPLANDTIAVSLYNTQAVLLTG
                     RWMPTKDLKIIGGAEWYQLQPSSSPSIGYNSIYDQTVFGGVATSALKPGYKQDNYVYY
                     LGANFDFAQRAPALAGLTASFGYYLTKSDAIEGPTVSTNSQAKIDTWTAMVDYKINKR
                     FDTYLAYTTNHFSGDKYPSATNYTNVTSYGAGLRMKF"
     gene            complement(3888..5171)
                     /locus_tag="VNZ84_00675"
     CDS             complement(3888..5171)
                     /locus_tag="VNZ84_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HWU65491.1"
                     /translation="MLRLKLSYLSLGLILSAYQLNLHAAGFALFEQGASGLGNAYAGA
                     SAVAEDASTIFFNPAGMTYLDGTQIVGALHLIKTNGQFDNKNSVPVAAYAPGGEGGDL
                     GSLAAIPNFFYKQDINEQFKLGLGISTPFGLKTEYDKNWLGRFQAVKSELKTININPS
                     LAWKLNDQWSLGFGISAMWAQAELTSAVNLSGFGLPESSVKNKGEDWGFGYNLGAIYQ
                     ITPDTRLGLSFRSKVEQHLKGEASSPFTLANGNPARTLNTDITADLTLPETLSFSSFS
                     RLDDRWDLLADITWTRWSQFKALSIMRDNGSNSAVGTTQEHWNNTLRYSLGLNYRYSD
                     AVKLRTGIAYDEEAIDNNHRTARIPGNDRIWLSFGASWQYSPQTRFDAGYAHLFIKEA
                     TIYDDQQTPAPSKGLIAGKYDGSADILSVQFTHLF"
     gene            complement(5254..7329)
                     /locus_tag="VNZ84_00680"
     CDS             complement(5254..7329)
                     /locus_tag="VNZ84_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU65492.1"
                     /translation="MIRLLPHLKIAHKLLLTELVMTLSMLLVFIVFAFGSHYHSLRSH
                     LIRDILVQMNVVANSIGPAVMFGEVSSAAKTLEPLTDNQNIRSIIILNQQQKPLQSLH
                     PEHPRAPAVWQWLYPYFHDMEVETPIIVNEKTVGTLHATIKQEQIVDVLISFAWSNAI
                     AVLAAALAGCLIMWRLNRNIVDPIHALQSFVSNITRNHHYDQRVKITSADEIGMLGHD
                     INNMLESIRQRDTQLQTELEHRKIIEEKLQFLAHYDKNTQLLNRHAFEEAITRKITAT
                     PDDRKHVYLLLLDLDRFKIVNDSFGHGTGDKLLQQVAHRLRGNLSQKDAIFRLGGDEF
                     AILLDASGRPAIEQVCKRIIQTISAQFIVDDHEIHIGVSIGIALLRPNFDSKTEVMKN
                     ADVALYQAKDAGRNTFRFYSETDEANTSELRTLREELHFALQNQELELYYQPIIHTQL
                     LKTIGFEALLRWRHPTKGLLTPDQFINLAEGSGLIISIGAWVIHQSLKQLAMWQEMFD
                     SDLFMNVNISSRQLDDDSLSNTIRHALDFYRVSPRTLNLEITESMVMRNIEAAKQILY
                     SLKQLGIGIAIDDFGIGHSSMNYLKQLPVDILKIDKQFTAGIPQDKVDIAIVDALVAL
                     AVSLNLKVVAEGVESTIQFEYLQYKLCHHVQGYLFSAALSASQTTIFMAEFPESIPAT
                     FRKIAGSTT"
     gene            complement(7337..>7542)
                     /locus_tag="VNZ84_00685"
     CDS             complement(7337..>7542)
                     /locus_tag="VNZ84_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HWU65493.1"
                     /translation="RLELQQVMQKDSVLTVVHSGNKFSEHGHVEISEQDKRYQFALHL
                     TSAKQAGIVFDTRLMNLATHIIN"
BASE COUNT         1898 a         1672 c         1959 g         2013 t
ORIGIN      
        1 tctaccgcct agacactggc aaccagattt tgcgggcatt tacgctatac cagggccaat
       61 ttcaccgcgc ggtaaaaaaa ggcaatttgc agacgctagg cttggttgag cgtggtatgg
      121 caacgattag ccaggcagaa ataacagccc tggagactaa atggaagggc agtcatttac
      181 agcatggtgt ctatcccaaa caggtgattt gggcattagc actcacctta ttagtcgcgg
      241 cgacactctc gttgctcgtt tatatgttac gcgtcgcggt aaaaagaaaa acggcagaga
      301 tcgaaagtaa acgcgaagag ttggagcttg aacgtttgcg gttgaccgac attattgatg
      361 cgactcgggc cgggacgtgg gaatggaata tacaaacagg tgagtgccaa tttaaccacc
      421 gttgggcaga aatgttaggg tatacattga acgcgttggc tccgtttaat gctgagatgg
      481 tgaaagcatt aacgcatcct gaagactgga aaaccgccat ggcactggtc gaacgccatc
      541 tcaaggggga gatggatttt tatgagtgtg atatccggat gcggcatcag gatggacatt
      601 gggtatggat cactgatcgt ggcaggctca tttcacgtac tatcaccggc gagccctgga
      661 tcatgcgtgg tacacacatt gatattaccg ataaaaagca ggcagatgcc gtgatttggc
      721 agcaggcaaa ctatgatagc ctgacccaac tccctaaccg gcattatttt caccagcaaa
      781 tcaaggaggc gttagctgcc gcgatagaca cacagcaacg cttaacctta ttgctgcttg
      841 atttggatcg cttcaaggag gtgaatgaca gccttggcca caaacgtgga gatgagctgc
      901 tgaaagaggt cgggcaacga attcgtcaat gcgtactgcc gccagcaacc attgccaggt
      961 tgggcggtga cgagtttgcc attattttgc cggaagcttt acaggcggat ttgcaagcct
     1021 tgtgtaacaa gttgatgcaa gatatttccg ctgcttatat tttaggtggc gagcgcatat
     1081 ttgtgaccgt gagtattggt atcagccaat atcctgaaga tgccgtcaat gccgaagaaa
     1141 tgattcagca tgcggaccag gcgatgtacg atgccaaagg ccgtggccgt aatagcctgc
     1201 ggtttttttc attggcaatc cgtgacgcct tgtcacagcg cgtgagcctt gcgcgcgatt
     1261 tgcgtacagc acttgagaat ggcgagttgc gcgattatta ccagcctatc gtcaatttgc
     1321 gtagcggaaa aattgtgaaa gcagaagcgt tgatgcgttg gcagcacccg gaacgtggcc
     1381 tgatcagtcc ggcgctgttt attccagtgg ccgaagaaac cggcgatatt attgccatgg
     1441 gtgactggat ttttaaccag gcggcgcaac atgcagcgca gtgggagtca ctggtaagcg
     1501 atgatgcctt cgcgataagt gtcaataaat cgccagtaca gtttgttgac aagatgggtg
     1561 caaaaaatga ctgggtacat gccttgcgta gcatgggcgt ggcaggtaaa cgtattgttg
     1621 tggaaattac cgaagggtta ctcctcaacc cggatcccgt ggttgaagaa cgattactac
     1681 agtatcgtga catggggata caggtggcca ttgatgattt tggcactggg tattcttcac
     1741 tctcgtacct gagtaaattc gatattgatt acctaaagat tgaccaatca ttcacgcgaa
     1801 acttgaatgc cggtaatgaa agctatgtgc tgtgtgaggc cattattgtg atggcgcaca
     1861 aacttggcct caaggtaatt gctgagggtg tggaaacggc gttgcagcgg gatttgctga
     1921 tcgctattca ttgcgattat gcacaggggt ttttctattc tccgccgatc ccggcagagg
     1981 cttttgaaca aatgctggta cagcaatcac gtgaagaggc agcggctgcc aaataagaac
     2041 ctacttttcc caacatgcca caccgtttag gatggcattt gcaatagttt ttagtatgat
     2101 gatatttgct cgtaggggct gaagcatgtt ggtttgcaga cgtaaatacc ttgtttgatt
     2161 agcccggttc gcgcactacc aataaaaaag actcaagtga ttacttgagt cttttttgtt
     2221 ttaagttgga ctttagaact tcatacgtaa gccggcacca tagctggtga catttgtgta
     2281 gttggtggca cttggatatt tgtcacctga aaaatggttg gttgtataag ccagataagt
     2341 gtcgaaacgc ttattgattt tgtagtctac catcgcggtc caggtgtcaa ttttggcttg
     2401 cgagttggtt gaaaccgtag ggccttcaat cgcatccgat ttggtcaggt aataaccgaa
     2461 agaggcagtc aggccagcca gtgcaggggc acgttgcgca aagtcaaagt tggcacccag
     2521 gtaatacaca tagttatcct gcttgtagcc aggttttaat gccgaagtgg ccacgccacc
     2581 gaacacggtc tgatcataaa tgctgttgta accaatcgaa ggggaggagg atggttgtaa
     2641 ctggtaccac tccgcaccac caatgatttt caggtctttt gttggcatcc atctgccggt
     2701 aagcaagaca gcttgagtgt tatacaagga cacagcaatg gtgtcatttg ccaatgggtt
     2761 accagctgca gcagtgccgg attttacaga gtcaaaaaat ctgtcatagg ccaattgaac
     2821 gccgaatgcg ctgctttcgt agcccaattg cgtggtgtag ccattgccgt gcgcagtacc
     2881 agccgaatta ccaaactgat tgatgactgc ataattaatt ttgtcatggc tttccaactc
     2941 gtacacattg gcgtacttga gcgagttttt catgcgtgag ttttcagaca caccgccacc
     3001 gccacccacc gtaccgcttt cacccaatgg tgaaaaactg tccgccttgc cgttagggtc
     3061 ataggtgacc gtgatttcaa agaacgggtt gtactgggta ccataggtta atttacccca
     3121 tgagccgcca tcgatcccgg cccaggcttg gcgcgtaaac aattcgccgt tcatggaaga
     3181 gttagcgctg acgctggagt tagcattcgt acctgagtta tcagccaatg tttgtgccgc
     3241 atcatgtaag cgcatatcca gcgtattaaa gccggcttca aactggtaaa tgaacttgaa
     3301 gtcatgatcc catgcttgca gcttagccac ttcgcccttg aaacccaggc gtgaaccttg
     3361 ccaaccacca ttcacccagt ttgtacggga ggtcactctg ctggtttttt ttgcgcctgc
     3421 ataaggataa aagttattgg ctaactcgta gttctcaggc agggagtggt tgatatgcgt
     3481 cagaccaata tctaccaggc cataaacttg aaagtaagaa ggatgatcgc ccaggtcgaa
     3541 gttgattcgg aatgggttgt cgtcattcat aaaacgggaa acaagagagg gtgactcttg
     3601 cgcagtggta ctggattgtt ctgccgcctg ggctgcaaat gcttggctga tggttaacgg
     3661 cagtaatgcc agtttgaaaa agtgcttgaa ggtcatcctc tatccttgta aacagtattg
     3721 cgggttattt cagaacaggg attttggagt ttttgtgtga aggttacgtg acatcaagct
     3781 tacacgaaac ttacatcatg aatttgcgca ttttaatccc cggataacca accatacgga
     3841 tgaaggtgtt aagggctggt tatcagagtt tctaggattg gtgcgggtta gaacaggtgt
     3901 gtaaattgca cgctgaggat atctgcactg ccatcgtatt tgccagcaat cagcccttta
     3961 ctcggcgcag gtgtttgctg gtcatcataa attgtcgctt ctttgataaa aagatgggcg
     4021 taacctgcat caaatctagt ctgcggacta tattgccagc tggcgccaaa agataaccaa
     4081 atgcgatcat tgcccgggat gcgcgctgtc ctgtgattat tatcgatcgc ttcttcgtca
     4141 taggcgatcc cggtacgcag ttttactgca tctgaatagc ggtaattcag gcctaacgag
     4201 tatcgcaatg tgttgttcca gtgttcttgc gttgtgccga ctgccgaatt ggaaccatta
     4261 tcgcgcataa tgctgagtgc tttaaactgg ctccaccgag tccaggtgat atctgccagc
     4321 aaatcccaac ggtcatccag gcggctaaag ctactgaagg aaagtgtttc cggtaacgtc
     4381 aggtcggcag tgatgtcagt gttaagcgtg cgtgccggat tgccattggc taaagtgaaa
     4441 ggcgatgagg cctcgccttt gagatgttgt tccacttttg accggaaaga gagccctaag
     4501 cgggtatcgg gcgtgatttg gtaaatagct cccaggttgt aaccaaatcc ccaatcctct
     4561 cctttatttt tgaccgatga ttcaggtaag ccgaaccctg acaggttaac ggcgctggtt
     4621 agctccgcct gggcccacat ggccgaaatg ccgaaaccca atgaccattg atcattgagt
     4681 ttccaggcca gtgatgggtt gatgttaatg gtcttcagtt cagacttaac cgcttgaaaa
     4741 cggcccaacc agtttttgtc atactcggtc ttgaggccga agggtgtgct aatgccaagg
     4801 cctaatttga attgctcatt aatatcctgc ttgtaaaaga agttgggaat agccgccagg
     4861 ctacctaaat ccccaccttc accaccaggt gcatatgctg cgactggcac actattttta
     4921 ttgtcaaact gaccattggt tttaatcaaa tgcaaggcac ctacgatttg ggtgccatcc
     4981 aggtaagtca tgccagccgg gttgaaaaaa atcgtgctgg catcttctgc aacggctgaa
     5041 gctccggcat acgcatttcc caggccactg gcgccttgtt cgaacaaggc aaagcctgct
     5101 gcatggaggt ttaattgata agctgaaagt atgaggccca gtgataggta agatagtttt
     5161 aagcgcaaca tgtgtttccc ctaggtatga ttttttatat gtttttagtt aaatctattt
     5221 ttaaagattt gctgactcat cgcctttcaa tcatcaggtg gtagagccag caattttacg
     5281 gaacgttgct ggaatgcttt ccggaaactc cgccataaaa atcgtggtct ggctggcaga
     5341 taaggctgcg ctgaacaagt agccttgcac gtgatggcag agtttgtatt gcaaatattc
     5401 aaactgaatc gtactttcca caccctcagc aaccactttc aggttcaggc tgacggccaa
     5461 tgcgaccagg gcatcgacaa tggcgatatc cactttgtct tgcggaatac cagcagtaaa
     5521 ttgcttgtca attttgagga tgtcgaccgg cagctgcttg aggtagttca tggatgaatg
     5581 gccgatgcca aagtcatcga tggcaatccc gatgcctaat tgctttaagg agtaaaggat
     5641 ttgtttggct gcctcaatgt tacgcatgac catggattca gtgatttcca ggttgagagt
     5701 gcgtggcgaa acacggtaaa aatccagggc gtggcgtatg gtgtttgaca ggctatcatc
     5761 atcgagttgg cgtgaagaga tattcacatt catgaatagg tcactgtcaa acatttcttg
     5821 ccacatggcc aattgcttga gtgattgatg tatgacccag gcgccgatgc tgataatcag
     5881 tccagaacct tcagccaggt tgataaattg gtctggggtc agcagtccct tggtcgggtg
     5941 gcgccatctg agcaaggctt caaagccaat ggttttcagt aattgggtat ggataatcgg
     6001 ctggtaataa agctctagct cttgattttg cagcgcaaaa tgtaattctt cacgcagggt
     6061 acgtagctcg gaagtattgg cctcatccgt ttcactgtaa aaacggaagg tatttcgccc
     6121 agcatccttg gcctggtaga gcgctacgtc agcatttttc atgacttcgg tcttggagtc
     6181 aaagtttggc ctgagcaagg caatcccaat gctgacgccg atatggatct catggtcatc
     6241 gacaataaat tgggccgaga tggtttggat aatacgtttg caaacctgct cgatggcagg
     6301 gcgtcccgag gcatccagca agatggcaaa ctcatctccg ccaagacgga agattgcatc
     6361 tttctgggat agattgccgc gcaagcggtg ggcgacttgt tgcaagagtt tgtcgccagt
     6421 cccgtggcca aatgaatcat tgacgatttt aaagcggtcc agatccagta gcaggaggta
     6481 aacatgcttg cggtcatccg gtgtagcagt gattttgcgg gtaatggctt cttcaaatgc
     6541 gtggcgatta agcaattggg tattcttatc gtaatgcgcc aggaactgca gtttttcctc
     6601 gatgatcttg cgatgttcca actccgtctg taattgcgta tcacgttggc gaatgctttc
     6661 cagcatattg ttaatatcat gccccagcat gcctatctca tctgcgctgg taattttgac
     6721 gcgttggtca taatggtgat tgcgcgtgat gttgctgaca aagctttgca gggcgtgaat
     6781 cgggtcaacg atattacggt tcaggcgcca cataatcagg cagccggcca aggctgcagc
     6841 caggacagca atagcgtttg accaggcaaa gctgatcagc acatccacaa tctgctcttg
     6901 cttaatggta gcatgcaggg tacctacggt tttttcattc acaataatcg gtgtttccac
     6961 ttccatatcg tgaaaataag gataaagcca ttgccataca gcgggtgctc gcgggtgttc
     7021 tggatgcagg ctttgtaggg gtttttgctg ctggttcagg atgataatgc tgcggatatt
     7081 ttggttgtca gtcagaggtt ccagggtttt agctgctgaa ctcacttcac caaacatcac
     7141 tgcaggccca atggaattag cgacgacatt catttgcacc aggatgtcgc ggataagatg
     7201 cgaacgcagg ctgtggtagt ggctaccaaa tgcaaagaca ataaagacca gcagcatgct
     7261 caaggtcatc accagctcgg tgagtaacaa cttatgtgca attttgaggt gcggtaacag
     7321 tcggatcata caagtatcaa ttaataatat gggtcgccag gttcatcagg cgagtatcaa
     7381 acacgatgcc tgcctgtttt gctgaggtca ggtgtagtgc gaactggtag cgcttgtcct
     7441 gctcactgat ttcgacatgg ccgtgctcag aaaatttatt accgctatgc accacagtta
     7501 ataccgaatc cttctgcatg acctgctgta attctagccg ct
//
LOCUS       DATMSK010000022        11910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_236465,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000022 DATMSK010000000
VERSION     DATMSK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236465"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VNZ84_00690"
     CDS             <1..344
                     /locus_tag="VNZ84_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWU65494.1"
                     /translation="QQLAKNLFLSGHRTPWRKAQEAIITFMLEKLMSKRRILEIYLNV
                     IEWGDGVFGAEAAARHYFHGNARTLGPAQAARLAAMIPNPRFYDVHRSTRYLGRHTGT
                     IQARMRLVKLP"
     gene            complement(490..3324)
                     /gene="uvrA"
                     /locus_tag="VNZ84_00695"
     CDS             complement(490..3324)
                     /gene="uvrA"
                     /locus_tag="VNZ84_00695"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWU65495.1"
                     /translation="MEFIKIRGARTHNLKNINLDIPRNQLVVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLARMDKPDVDLIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLYARAGDPECPDHGIKLEAQTVSQMVDSVLKLPEDTKLMILAPVVSNRKG
                     EQLDLFDTLKAQGFVRLRIDGKIYEMDSLPQLAKTTKHSVDVVVDRLKVREDMKQRIA
                     ESFETALRLADGKAIALEMDTEKEHLFSAKFSCPVCDYSLAELEPRLFSFNNPMGACP
                     KCDGLGQVTFFDPKRVVAFPHLSLASGAIKGWDKRNQFYFQMLASLSQHYNFDLEAPF
                     ETLPEETQQILLYGSGREQITFKYLNERGTFFSKSHTFEGIINNLQRRYRESDSTAVR
                     DELAKYINATACPDCGGTRLRREARHVKVGERNIHQICEVPLKQALHFFETLQLSGQK
                     LAIADKIVKEIESRLKFLTNVGLDYLSLSRSAETLSGGEAQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNDRLLETLKRLRDLGNSVIVVEHDQDAILLADFIVDIGPGAGEHG
                     GQIVSSGTAAEIQADPNSLTGQYISGKKQIQFKLPRTQPDPARMIKLNGTTGNNLKNV
                     SIEIPVGLLTCVTGVSGSGKSTLINDTLYRAVATHLYGSSTEAAPHDSIEGLEFFDKV
                     VDVDQSPIGRTPRSNPATYTGLFTPIRDLFASVPESRARGYGPGRYSFNVKGGRCEAC
                     QGDGVIRVEMHFLPDVYVPCDVCKGQRYNRETLEIQFKGKNIHEVLEMTVEQAHQFFN
                     AQPVIERKLKTLLDVGLGYITLGQSATTLSGGEAQRVKLSLELSKRDTGRTLYILDEP
                     TTGLHFADIQLLLDVIHRLRDAGNTVVIIEHNLDVIKTADWLIDMGPEGGDGGGTVVG
                     VGAPETLAKNTASYTGRYLTPLLEHLAVKG"
     gene            3402..4796
                     /locus_tag="VNZ84_00700"
     CDS             3402..4796
                     /locus_tag="VNZ84_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU65496.1"
                     /translation="MQFSEKMTRIETRATAALASIYGLRMLGMFLILPIFAIYAETLQ
                     GGDNHALIGLALGAYGFMQVIFQLPFGMASDRFGRKKLIYLGLIMFAVGSVFAATAQD
                     IYMVIIGRAIQGAGAISAVVTALVADSTREEHRTQAMAMIGATIGVTFAVSLVAGPIL
                     NQWIGVSGIFWLTAALSIAAIAVVKFAVPEPLHAHFHSDAQAAPAKIREVLRDTQLLR
                     LNFGTFCLHGAQMAMFMVVPFAIKNSTGMNENTHWQIYLPVLVLSFILMVPAIIYAEK
                     KAKLKPVFVTAVGLMLLTQVAFIFSLNSLWGIVATLFSYFVAFNILEASLPSLISKMA
                     PVAAKGTAMGVHNTAQSFGMFLGAALGGWLSHSYGSATVFVFCAALMLIWFALALGMQ
                     APPSVKTKMFHLQAGYNDEQAAKLVQDIRAMEGVYEAVAIPSETMLMVKLENQRSKEF
                     QATLSQAIMNRIGE"
     gene            4804..5328
                     /gene="ssb"
                     /locus_tag="VNZ84_00705"
     CDS             4804..5328
                     /gene="ssb"
                     /locus_tag="VNZ84_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWU65497.1"
                     /translation="MASVNKVILMGNLGRDPEVRYMPNGEAVANFSIATTENWKDKSG
                     VRQEKTEWHNIVMYRRLAEIAGEYLKKGRPVYIEGRLQTRKWEKDGVTRYSTEIVADQ
                     MQMLGTGREGGSAAFDGGDMDQGGGMDDYNQAPPRQQSAMGGGNAGGANRNAFQPASK
                     PAGNFDDFDDNIPF"
     gene            complement(5425..7050)
                     /locus_tag="VNZ84_00710"
     CDS             complement(5425..7050)
                     /locus_tag="VNZ84_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HWU65498.1"
                     /translation="MTPSRANHQYQRGAIGLVGVMLLLSAVLLTALVLDSGRLWMQKK
                     QLQSIADLAAISASRHLGCSADLSNVTQMAQLAAANNGFDGQLSASPNLVLLGRLQTV
                     QGIRQFTADGSHGAVFVRATREVPSSLVAGGLFGGTIILRAEAVSASDPPLAAFSAGS
                     FTASLSSEQSTLLNGLLGGMLGAPLNLDVLAYRGIAETKITLQNLLSVSGQVGSLQEL
                     LNTNMQIGDLLELVANAAGTNGTVDLQALSAIQEIAAVAVKNPPVKLGDVLALTTPDT
                     NAAATVALNALSLITTAVMIANGTNAVTLPLGLNISSITNVNAQIIIVEPPQLIIGPA
                     AGQGTLCTVARTAQVRARVGVLVNIPLLASVDLALNVEVAQGSASLRSIQQNDGESEV
                     EIEGSPGIAALTLSNNAGTGPARVSTLLNIPVATIGLNLPLQPTAAQMLEFSVPNPVR
                     NYLPQTQTISSPLGSSLANVLGQANTLDVTVLSILNLGLLNNVISTIISPLLSEIGRV
                     LLDPLLNLLGIRIGGMDITLEDLQYRQVKPLAI"
     gene            complement(7031..7198)
                     /locus_tag="VNZ84_00715"
     CDS             complement(7031..7198)
                     /locus_tag="VNZ84_00715"
                     /inference="COORDINATES: protein motif:HMM:NF023687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3613 domain-containing protein"
                     /protein_id="HWU65499.1"
                     /translation="MSPVQQWLELQRSGKSASAQPQPAPGEVMEKVHQRYLKSFEKPI
                     PEFYTHDPVTR"
     gene            complement(7327..8016)
                     /locus_tag="VNZ84_00720"
     CDS             complement(7327..8016)
                     /locus_tag="VNZ84_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWU65500.1"
                     /translation="MNMISKSIILAWVLIHSCSDAFSADTNHDSVRHLKTTIDKACTA
                     ISAEESTKLGLIQQILAEGKPHAALAHLDASGIKHEQAALLRANALRQTGRVGQAQII
                     YDQLTTSCVSGYAYRGLGLIASTAGQIQESLSYLEAASRALPTEHTIRNDHGYLLMQS
                     GNYQLALHEFLTALELAPDYRQAANNLVLLLYRQGDTVKAEAFAKQFGMVAEEMQQLK
                     KMTHASNGTMP"
     gene            complement(8016..8930)
                     /locus_tag="VNZ84_00725"
     CDS             complement(8016..8930)
                     /locus_tag="VNZ84_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWU65501.1"
                     /translation="MNIFWLIATILMLLAAGFLLYSAQKDNHPKHIHRRFREALQQEG
                     IHISARFTPLNLTEIIQATLAKISHSEMEEMQHLLLQAGWPGAKAIFTFLLLAWLLPV
                     AFGLLAAFYAILTQNSAVNVLFHLVFAFAATFVLIRRLLRWKAQKRREAIRKEVIPFL
                     HLLRMLFEAGLSLEHILLVIEQQGRDLIPNLAKELEGVIKRIHTGQDRADALVEMAAP
                     LEVTELNDTIAMLKQVTRYGGNVRDSLAEYTTLTEQRQVSQLREYVSKLSAKMTIVMI
                     LFLFPALMAFLAGPGFIGLSKALKGVGG"
     gene            complement(8933..9850)
                     /locus_tag="VNZ84_00730"
     CDS             complement(8933..9850)
                     /locus_tag="VNZ84_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWU65502.1"
                     /translation="MSVLAVSFIVGAIALLLLAIWMLKHSKEARFRERMMHNFREVLP
                     YGTTVSGMAPGIPPPFWHVLKIRASIYAGFELRKKHAVVIPLAFISVGLLGWLTYGLL
                     GATLTFFITVFVFGFLLPYNRLRRRQAQIIAQIPLFIDQVLRSLSTGRSLESAIRFGA
                     GEAPPPLRYVLDRVIRAADLGADMVESLAEAAKLHDLRELNLIALSMRISNNYGSSPR
                     EMLDSVVRMIRQQELARRELSAMTGETRMSAWVLGLTPLVIAVYIMLMNPSYLDILLQ
                     DPTGQSMMTTALVLQGMGAFILWRMLRSV"
     gene            complement(9847..11112)
                     /locus_tag="VNZ84_00735"
     CDS             complement(9847..11112)
                     /locus_tag="VNZ84_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HWU65503.1"
                     /translation="MHVQLLGFDYGMQQAQDYQELKFQLHQHLIAEIERDHIGLLNLV
                     RNEIATYVQEKVIAYVADRHLPISGYDLENLVEEVVDELAGYGPLESLIRDDRINDIL
                     VNGPKKIFIERDGRLESTTLRFIDDDHVLRVIRRILAHLGRRIDEASPMVDARLPDGS
                     RVNAIIPPLALDGPCLSIRKFRKQPLLSADLLQYGTVDNEMLAFLTAAVERRCNLIVS
                     GGTGAGKTTLLNVLSRFIPHGERIVTIEDAAELQLEHDHVVRLETRPSNIDDSGEITA
                     RELVKNSLRMRPDRIVLGEVRGEEVMDVLQAMNTGHDGCMSTVHANSPEDAMLRFEML
                     AGLAKFQGTESTLRKMIAAAIELVVQIARTANGQRKVVSITEIVGIRDGHFVLNELYA
                     YDTNTGHFSHPGVRPCNQKLQDLPFRRPL"
     gene            complement(11125..>11910)
                     /locus_tag="VNZ84_00740"
     CDS             complement(11125..>11910)
                     /locus_tag="VNZ84_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65504.1"
                     /translation="VISARPGSDSPMLALHLALAVQETEPTLLLDLGVPHGDAMLYLG
                     LTSSYSFIDAIRSLRRIDSTLIQTGFGKHKSGLTVLSMPEDPWVGAQFTAADVSVLLR
                     SLRRHFPRIVVNLGGMVGSDFLMLLLGNADQIVLLVEQSVPSCRQNMQLIKHLREEKI
                     LLTNAGVVVDRYLSKLPPDAESIAQSFGLPLLATLSPSGMARLATMNSGESMFELSPH
                     DPYSLSVRKLAEKLLEAPIPQTENKQGMLHKLISRVTAKSEKF"
BASE COUNT         2898 a         2927 c         3019 g         3066 t
ORIGIN      
        1 gtcagcaatt agccaaaaac ttgtttttat ccggacaccg tacgccctgg cgtaaggcgc
       61 aagaggcaat cattaccttc atgctggaga agctcatgag taagcgccgc atcctcgaaa
      121 tttatctgaa tgtaattgaa tggggggatg gtgtatttgg tgccgaagcg gcggccagac
      181 attactttca tggcaatgcc aggaccctgg gaccagcaca ggcggcgcgg ttggccgcca
      241 tgatccccaa tcctcgcttc tatgatgttc atcgcagtac acgctacctg ggccgccata
      301 cagggaccat acaggcgcgt atgcgattgg tgaaattacc ctgattttaa ggctttgttt
      361 gggttattga accaaaatgc cgccacatac aatgtttggt ataaatatat gtatgtggct
      421 aaaccacatc cgtattgatg cgccactgca tgaatgctgg tgcctcaggg ggccggttgc
      481 atgttgacct tagcccttaa cagcgagatg ttccaacaag ggtgtgaggt aacggccagt
      541 ataactggcc gtattttttg ccagtgtctc gggcgcacct acacctacca ccgtcccgcc
      601 gccatcgcca ccttcaggac ccatgtcgat cagccagtca gcggttttaa tgacatccag
      661 gttatgctca ataatcacca cggtgttgcc cgcatcgcgc agtctgtgaa tcacatccag
      721 cagtaattga atatctgcaa aatgcaggcc ggtcgtcggt tcatccagga tatagagcgt
      781 acgcccggta tcacgcttgc tgagttcgag agacagctta acgcgttgtg cttcaccgcc
      841 tgacagcgtg gtcgcggatt gtcccagcgt aatgtagccc aggcccacgt cgagcagcgt
      901 tttcagtttg cgctcaatca cgggttgcgc attaaaaaat tgatgcgcct gttccacggt
      961 catttccaac acttcatgga tgttcttgcc cttgaactga atttctaacg tttcgcggtt
     1021 atagcgttgg cctttgcaca catcgcaagg cacatataca tctggcaaga agtgcatttc
     1081 aacacggatc acaccatcgc cttggcaagc ctcgcagcgg ccgcccttga cgttgaatga
     1141 gtaacgccct ggaccataac cgcgcgcacg gctttccggt acgctggcaa acaagtcacg
     1201 aataggtgtg aataaaccgg tataggtggc cgggttagag cgtggcgtac ggccgatagg
     1261 gctctggtcg acatcgacta ctttgtcgaa aaactctaag ccttcaatgc tgtcatgcgg
     1321 tgctgcttca gtgctgctac catacaaatg tgtggcgacg gcacgataca gcgtatcgtt
     1381 gattaatgtc gattttccgc tgccggaaac gcctgtcacg caggtgagta gtcccaccgg
     1441 aatttcgata ctgacgtttt tcaggttatt acccgttgtg ccgttcagtt taatcatgcg
     1501 tgccgggtct ggctgcgtgc gaggtaactt gaactgaatt tgttttttgc cgctgatata
     1561 ttgaccggtc aaggaattcg ggtcagcctg aatttcggcc gcagtaccag aagagacaat
     1621 ctggccaccg tgttcaccag ctccagggcc gatgtccaca ataaagtcgg ccagtaaaat
     1681 cgcatcctgg tcatgctcta cgacaatcac gctgttaccg aggtcccgca ggcgtttcaa
     1741 tgtttccagc aaacggtcgt tgtcgcgctg atgcaggccg atagaaggtt catccagcac
     1801 atacatgaca ccggtcaagc cgctgccaat ttgcgaagcc aggcggatac gctgcgcttc
     1861 gccacccgat aaggtttctg ccgagcgcga cagcgacaaa taatccagac ccacattggt
     1921 aaggaacttc aggcggcttt caatttcctt gacgatttta tcggcaatgg ccagtttctg
     1981 gccgctcagt tgcaaggtct caaaaaagtg caatgcctgt ttcaaaggca cttcacaaat
     2041 ctggtgaata ttgcgctcac caacttttac gtgacgggct tccctgcgta agcgggtacc
     2101 tccacaatcc ggacaagcgg tagcattgat gtatttagcc agttcgtcac gtacggcagt
     2161 agagtcactt tcgcggtaac ggcgttgcag gttattgata atgccttcaa aagtatgtga
     2221 cttgctaaag aaggtcccgc gctcattcag gtatttgaat gtgatttgct cacggccaga
     2281 gccatacaac agaatttgct gggtctcttc cggcaaggtt tcaaatggcg cttcaaggtc
     2341 aaagttgtaa tgttggctga ggctagccag catctggaag tagaactggt tgcgcttatc
     2401 ccaaccttta atggcaccgc tagcaagcga taaatgcgga aaagcaacta cgcgcttggg
     2461 gtcaaaaaac gtgacctggc ccaaaccatc acatttgggg caagcgccca tggggttatt
     2521 aaaactgaat aaacgcggct caagctctgc cagtgagtag tcacacaccg ggcaggagaa
     2581 cttggctgag aacaagtgtt ctttttcagt gtccatttcc agcgcgatcg ctttgccgtc
     2641 ggccaggcgc aatgcggttt caaaagactc tgcaatacgc tgcttcatgt cttcccgcac
     2701 tttgaggcgg tcgaccacca cgtccacgct atgtttagtg gttttggcca attgcggcag
     2761 gctatccatt tcgtagatct tgccatcgat gcgcaggcgt acaaaccctt gtgccttgag
     2821 cgtatcaaac aaatccagct gttcaccttt acgattcgat accacaggcg ccaagatcat
     2881 cagtttggtg tcttcaggca acttgaggac actgtccacc atctggctaa ccgtttgtgc
     2941 ttcgagtttg atgccatgat ccgggcattc ggggtcgccc gcacgtgcat agagcagacg
     3001 caggtagtcg tggatttcag tcaccgtgcc aacggtggag cgagggttgt gcgaggtgga
     3061 tttttgctcg atggaaatcg caggcgacaa gccctcaatc agatcaacat caggtttgtc
     3121 catgcgcgcc aggaattggc gcgcataggc tgataagctt tcgacgtagc ggcgctgtcc
     3181 ttctgcatag agcgtatcaa aggccaatga tgattttcca gaaccggaga ggccggtcac
     3241 tacaaccagc tggttgcgcg gaatatccag attgatgttc ttcagattat gggtgcgcgc
     3301 cccgcgaatt ttgataaatt ccatgatact ttcacgaaca gacagccaac ctgataatat
     3361 acgcaattat tcaaaattaa gccaagtttt cttacagatt catgcagttc tccgaaaaaa
     3421 tgacccgaat agaaacgcgt gccactgctg cattggcgtc tatttacggg ctccgtatgt
     3481 tgggcatgtt tttgattctg cctatttttg cgatttatgc cgaaaccttg caaggcggtg
     3541 ataaccatgc cttgattggg ctggcactgg gtgcctatgg ttttatgcag gtgattttcc
     3601 agttaccgtt tggcatggca tctgatcgct ttggccgtaa aaaactgatt tacctgggct
     3661 tgatcatgtt tgcggtgggc agtgtgtttg ccgcaaccgc acaagacatt tacatggtga
     3721 ttatcggtcg cgcgattcag ggggcgggtg ctatttctgc ggtggtgact gcgctggtgg
     3781 ccgattccac gcgtgaagaa caccgcacac aagccatggc gatgataggc gctaccattg
     3841 gtgttacttt cgcggtgtcg ctagtggctg ggccaattct gaatcagtgg ataggcgtat
     3901 ccggtatttt ctggctgacg gcagccctgt cgattgcggc gattgcggtg gtgaaatttg
     3961 ccgtgccaga gccgttacat gcacactttc actcagatgc gcaggcagcg ccagccaaaa
     4021 ttcgtgaagt actacgagat acccaattgt tgcgcctgaa ttttggcacg ttttgcctgc
     4081 atggcgcaca aatggccatg ttcatggtgg tgccgtttgc gattaaaaat agcacgggca
     4141 tgaatgaaaa tacccattgg caaatttatc tgccggtgtt ggtgctctcg tttatcctga
     4201 tggtccctgc cattatttat gccgagaaaa aggctaaatt gaaaccagtg tttgtcaccg
     4261 cagtaggtct catgttgctg acacaagttg cctttatatt cagtctaaat agcttgtggg
     4321 ggattgtcgc gaccttgttc tcgtattttg tcgcgtttaa tattcttgaa gcctccctgc
     4381 cctccctgat ttcaaaaatg gctccggtgg ctgccaaagg caccgccatg ggcgtgcaca
     4441 ataccgccca gtcattcggg atgtttctgg gcgcagccct gggtggctgg ttatcacaca
     4501 gctatggcag tgccacggtc tttgtgttct gcgcagcatt aatgttgatc tggtttgcac
     4561 tggcacttgg catgcaagca ccccccagtg ttaaaacaaa aatgttccat ttgcaagcgg
     4621 ggtataatga tgaacaggct gcaaaactgg tgcaggatat acgcgccatg gagggcgtct
     4681 atgaagccgt tgccattccc tcggaaacca tgctcatggt aaagttggaa aatcagcgca
     4741 gcaaagaatt tcaggcgact ttgtcgcaag caatcatgaa tcggataggg gagtagtgac
     4801 actatggcat cagtcaacaa agtgattttg atgggcaatc tggggcgtga cccggaagtg
     4861 cgctatatgc caaacggtga agccgtggct aactttagta ttgccaccac cgaaaactgg
     4921 aaagacaaat ccggcgtgcg ccaggaaaaa accgagtggc ataacattgt catgtaccgc
     4981 cgcttggccg aaatcgcagg cgaatacctg aaaaaaggcc gccctgtcta catcgagggc
     5041 cgtttacaaa cccgcaaatg ggaaaaagat ggcgtgactc gctatagtac cgagatcgtc
     5101 gctgaccaga tgcaaatgct gggaaccggc cgtgaaggcg gtagtgccgc gtttgatggt
     5161 ggcgatatgg atcaaggcgg cgggatggat gattacaacc aggcacctcc acgccagcaa
     5221 tcggccatgg gtggcggtaa tgcgggtggc gcgaaccgca acgcattcca gccagctagc
     5281 aaaccagctg gcaattttga cgattttgat gacaatattc ctttttaaca gtcaccaaag
     5341 caattatgta aataactatt tggagccaat atatttggct ccattttttt gtttacatga
     5401 tcttcagtga ggcatactgt ttttctatat tgccaatggc ttcacctggc gatattgcag
     5461 atcctctaag gtaatatcca tgcccccgat acgaatccct aataagttga ggagagggtc
     5521 tagtaatact ctgcctattt ctgataatag cgggctgata atcgtggaga tcacgttgtt
     5581 caatagccca agattcaaga tgctcaatac ggtcacatcc aacgtgtttg cttgccctag
     5641 gacatttgct aaactgcttc caagcgggct ggaaattgtt tgtgtttgtg gtaaataatt
     5701 acggacgggg ttgggcactg aaaactccag catttgcgct gcagttggct ggagaggaag
     5761 attcagccca attgttgcaa caggaatatt cagtaatgtg gagactcttg ctgggcccgt
     5821 tccagcattg ttgctgagag ttaatgcggc aatccctggg ctgccttcta tttcgacttc
     5881 cgactcccca tcattttgtt gaatactacg caagcttgca gacccttgcg ctacctcgac
     5941 gttcagtgcc agatcgacac ttgcaagcag aggaatattc accagtacac ctacacgtgc
     6001 tctgacctgt gccgttcttg caacggtgca taatgtgcct tgtccagcgg caggccctat
     6061 aatcaattga ggtggctcga cgataatgat ttgagcattt acgttggtaa tagaagagat
     6121 attcaagcct aaaggcaggg taactgcatt tgtaccattg gcaatcataa cggctgttgt
     6181 gattaaagac agagcattga gtgcgactgt agcagctgca ttagtatcag gcgttgtaag
     6241 ggcaagcaca tcccctagtt taaccggagg atttttcact gctactgcgg caatctcttg
     6301 tatggctgat aatgcttgca aatcaacggt cccgtttgtc ccggcagcat tggcgactag
     6361 ctccagtaaa tctccaattt gcatattggt gttcaacaat tcttgcaatg aaccgacttg
     6421 cccggaaact gagagcaggt tttgtaatgt gattttagtt tctgcgatgc cacgataggc
     6481 aagcacatcc aggttgagtg gtgcgcccaa catgccacct aatagaccat ttaataatgt
     6541 ggattgttcg ctactgaggc tggccgtaaa gctccctgca ctgaatgctg cgagtggtgg
     6601 atcggatgct gagacggctt cagcgcgcaa aatgatggtt ccgccaaata aaccgccagc
     6661 caccagacta cttggtacct ctctggtcgc ccttacaaac acagcgccat gactgccatc
     6721 tgcagtaaat tgccgtatgc cttgtaccgt ttgtagtcgc cccagcaaga ctaggttagg
     6781 gcttgcagaa agttgtccgt cgaagccatt attggcagca gccagttgcg ccatttgggt
     6841 cacgtttgaa agatcggcgc tacatcccag atgacgagag gcgctgatag cagctagatc
     6901 ggcgatggac tgcagctgtt ttttctgcat ccaaagacga ccgctgtcta gcaccaaagc
     6961 agttaaaagc actgcgctta ataacagcat tactccaaca agtccgatag ctccgcgttg
     7021 atattgatga ttagcgcgtg acggggtcat gggtatagaa ctcgggaatg ggcttctcaa
     7081 agctcttaag gtaacgctga tgcacttttt ccatcacttc gccgggagcc ggctgtggct
     7141 gcgctgaggc ggatttgcca gagcgttgca attccagcca ttgttgtaca ggtgacaact
     7201 cgtgcactgc tgttgatggt atggcttgtt gtgttgttat ctcctcgcca tttgctatgg
     7261 cactactgag attgaaaaat ccaaataata tgagaaaaaa ttgtttcatg atggccacct
     7321 tccggtttag ggcattgtgc cgttgcttgc gtgtgtcatt ttcttgagtt gttgcatttc
     7381 ttcggcaacc attccaaatt gcttcgcaaa ggcctcggcc ttaacggtat caccttgccg
     7441 gtaaaggagt agaacaaggt tattggccgc ttgtcgataa tcaggggcaa gttccagagc
     7501 cgtcaaaaac tcatgtaggg caagttggta gttgcctgat tgcataagca gatacccgtg
     7561 atcatttcgt atggtgtgtt cggtgggcag tgcgcggctg gcagcttcaa gatagctgag
     7621 agattcttgt atttggccag ctgtgctggc aatcagtccc aagccacgat aggcatagcc
     7681 tgacacgcaa cttgtggtta actgatcata aattatttgt gcttgcccta cccgtccggt
     7741 ttgacgcaat gcatttgctc gtagcaacgc tgcttgttca tgtttgatcc cggatgcatc
     7801 aaggtgtgca agggctgcat gtggcttgcc ttcagccaat atttgctgaa tcaggccgag
     7861 tttggtggac tcttcagcag atatcgctgt acaagctttg tctatcgtcg tcttgaggtg
     7921 ccgtacagag tcgtggttgg tgtcggcgga aaaggcatct gagcagctat ggatcagtac
     7981 ccatgccagt attatcgatt ttgagatcat attcattatc caccgactcc ttttaacgct
     8041 ttgcttagcc caataaaacc aggtcctgca aggaatgcca tgagcgccgg gaacaaaaac
     8101 aggatcatga cgatggtcat ttttgcagat agtttgctga catattcgcg caattgtgaa
     8161 acctggcgct gctcagttaa tgttgtatat tcggccaatg aatcgcggac attaccgcca
     8221 taacgtgtca cctgcttaag catggcaatc gtgtcattca actcggtcac ctctagtggt
     8281 gccgccattt ctaccaacgc atctgccctg tcctgtcctg tgtgtatacg tttgatcact
     8341 ccctccagtt ccttggcaag gttgggaatc aggtcacgac cttgttgctc aataaccagc
     8401 aaaatatgct ccaaagacag gcctgcttca aagagcatac ggagcaggtg taaaaatgga
     8461 atgacttctt tgcgaatagc ttcacgtctt ttttgtgctt tccaacgtag taaacggcgt
     8521 atcaggacaa atgttgctgc aaaagcaaac actaaatgga atagtacatt caccgcggag
     8581 ttctgcgtca atatggcata aaaggcagcc agcaggccga acgctaccgg cagcagccag
     8641 gccaatagca gaaacgtgaa gatggccttg gctcctggcc agccagcctg taagagtaag
     8701 tgttgcattt cctccatttc gctatgtgag atcttggcga gcgtggcctg gatgatttct
     8761 gtcaaattga gtggggtgaa tcgtgctgag atgtgaatac cttcttgttg caaagcttca
     8821 cgaaaacgcc ggtgaatatg cttgggatga ttgtcttttt gcgcactgta gagtaaaaac
     8881 ccggcggcca gcagcatgag tatggtggcg atcaaccaaa agatattcat ctctagacgc
     8941 tccgcaacat ccgccagaga atgaatgcgc ccatcccttg cagcacaagg gctgttgtca
     9001 tcatggattg accggtagga tcctgtagta ggatgtccag atagctcgga ttcatgagca
     9061 tgatgtagac cgcaatgaca aggggcgtta atccgagcac ccaggctgac attcttgttt
     9121 caccagtcat ggcggataat tcccgacgtg ccagctcctg ctgccgtatc atcctgacca
     9181 cgctgtccag catctcacgc gggctgctac catagttatt gctgatacgc atggacaagg
     9241 caatcaggtt tagttcacgc aagtcatgta gctttgccgc ttctgccagg ctctctacca
     9301 tatctgcgcc tagatcagct gcgcgaatca cacgatccag tacatagcgt agtggcggag
     9361 gcgcttcgcc tgcaccaaag cggatagcgc tttccaggct acgtccggta ctcaggctac
     9421 gtagtacctg atcaataaaa agaggaatct gggcaatgat ttgtgcctgg cggcggcgca
     9481 aacggttgta gggcagcaaa aagccgaaca caaaaaccgt tataaaaaac gtcagtgtgg
     9541 cccctagtaa accatatgtc agccatccga gcagtcccac agaaataaag gccagcggga
     9601 tgacaacagc atgtttcttt cgtaactcaa atccggcata gatagaggcg cgaattttca
     9661 acacatgcca aaaaggtggc ggtatacctg gggccattcc cgaaaccgtc gtgccataag
     9721 gcaacacctc ccggaaatta tgcatcatgc gttcacggaa gcgggcctct ttgctatgtt
     9781 tcagcatcca gatagccagc agcaataatg cgatagctcc cacaatgaag gagaccgcta
     9841 agacactcat agcggtctcc tgaaaggtaa atcctgcaat ttctgattgc atggtctcac
     9901 gccagggtgg gagaagtggc ctgtgtttgt gtcataggca tagagttcat tcagcacaaa
     9961 gtgaccgtcg cgtatgccga caatctcagt aatcgaaacg actttgcgtt gtccgttagc
    10021 tgtacgggca atttgtacga caagctcaat ggctgcagca atcattttgc gtaatgtact
    10081 ttctgtgcct tgaaacttgg ctaatcctgc caacatttca aagcgcagca tggcatcttc
    10141 cgggctattt gcatgtaccg tactcatgca tccgtcatgt cctgtattca tggcctggag
    10201 tacatccatg acctcctcgc cgcgtacctc acctaacacg atgcggtcag gccgcatgcg
    10261 taaagagttt ttcactagct cgcgcgctgt gatttcgccg ctgtcgtcaa tattcgacgg
    10321 cctggtttca aggcgtacta cgtggtcgtg ttccaattgc aattcggctg cgtcctcgat
    10381 agtcacaatt cgctcgccgt ggggaataaa acggctcaaa acattcaata aagtggtttt
    10441 tcccgcacca gtaccaccac tgacgatgag gttgcaacga cgttcaacgg ccgcagtcag
    10501 aaatgccagc atttcattgt ccaccgtgcc atattgcaac aggtcagcag agagcaaggg
    10561 ctgtttacgg aatttacgga tagataggca tgggccatcc aatgcgagtg gcgggataat
    10621 cgcgtttacg cgactaccat ccggtagccg cgcatctacc attgggctgg cttcgtcaat
    10681 gcgccttccc aagtgcgcga gaatacgccg gatgacgcgt aacacatggt catcatctat
    10741 gaaccgtaaa gtcgttgatt cgagtctgcc atcccgctct ataaaaattt tcttgggtcc
    10801 attaacaaga atgtcattaa tgcggtcatc ccttatcaaa ctctcaagag ggccgtatcc
    10861 cgctaactca tctacgactt cttcgaccag attctctaaa tcatagccac tgataggtag
    10921 gtggcgatca gcgacgtaag caatcacttt ctcttggaca taagtagcga tttcattacg
    10981 tacaagattg agcaatccaa tatgatcgcg ttcaatttcc gcgatcagat gctggtgtag
    11041 ctgaaactta agctcttggt aatcttgtgc ttgttgcatg ccataatcaa atccaagtaa
    11101 ttgaacgtgc atcatgcaat ccttttaaaa tttctcactt tttgcagtta ctctagaaat
    11161 cagcttgtga agcatgcctt gtttattctc agtttggggg atgggtgctt ccaataactt
    11221 ttcggccagt ttgcgtacac ttaagctgta agggtcatgt ggtgataatt caaacatact
    11281 ttcgccagag ttcatggtgg ccaggcgtgc catgcctgaa ggcgagagtg tggccagcaa
    11341 tggcaagcca aaagattgag cgatgctttc ggcatcgggt ggtagttttg aaagatagcg
    11401 atccaccacc acacctgcat tggtcaacag aatcttttcc tcacgtaagt gcttgattag
    11461 ttgcatattc tgcctgcaac tggggacgct ctgttcaact agcaaaacaa tttgatcagc
    11521 attaccgagt agcaacatta aaaaatccga gccgaccatt ccccccaggt ttactacaat
    11581 tcgtggaaaa tgccggcgta ggctacgcaa aagcacgctg acatctgcag ctgtgaattg
    11641 cgcacccacc caagggtctt caggcattga gagtaccgta aggccactct tgtgcttgcc
    11701 aaaaccagtt tgaatcaagg tggagtctat tctgcgcagg ctgcggatgg catcgataaa
    11761 actgtaggaa gacgtcaggc ccaggtatag catggcatcc ccatgaggca caccgagatc
    11821 tagcaggagt gttggctctg tttcttgcac tgccaaggcg aggtgtagtg ccagcatggg
    11881 ggagtcacta cctggccgcg cactgataac
//
LOCUS       DATMSK010000023         7197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_142980,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000023 DATMSK010000000
VERSION     DATMSK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142980"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VNZ84_00745"
     CDS             complement(<1..426)
                     /locus_tag="VNZ84_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWU65505.1"
                     /translation="MSSVRYCVNGKLVSGIAPTNRGFAYGDGIFRTMRLLNGDLLDWP
                     LHYQTLVADCSKIQIVCPSAELLMQEFKHLMATGEGDELASGIVKIIITRGDGARGYA
                     PPAICEPTRVLIHSPLPVYAPEIYHTGVALYTCETRLASQ"
     gene            628..1077
                     /locus_tag="VNZ84_00750"
     CDS             628..1077
                     /locus_tag="VNZ84_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65506.1"
                     /translation="MSVSNIQSNLSALLNKAYNTDSSATSASDAISQLVKETSSSTDN
                     SAAASASSLVTLGGADTTTGTYTAKGLMQQLRQYQLSNASLLFGNSDDSSQDNTGLTG
                     LMGTSSDTDLASMSEDWVSTISENPGKASVMVASTQNDTLKTILGNN"
     gene            complement(1100..1594)
                     /locus_tag="VNZ84_00755"
     CDS             complement(1100..1594)
                     /locus_tag="VNZ84_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWU65507.1"
                     /translation="MVANVHSPVTDLLKQHEIDFQVIEIPLNEDKKPIRHLEELLSAQ
                     GLDPQCVVRSVVFKGENSGFCLLAVAGGGRADWALLRAQLNERKCRMAEYDEVPEATG
                     YVVGAVPPIALPASLPVLVDSSVSRYETVVIGSGVLGFALALRSADLLQLLGDKVQGH
                     FVKE"
     gene            complement(1642..1995)
                     /locus_tag="VNZ84_00760"
     CDS             complement(1642..1995)
                     /locus_tag="VNZ84_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HWU65508.1"
                     /translation="MTDSIDALRTRVNQFVEERDWAQFHSPKNLAMAMIVEAAEVVEH
                     FQWMTEADSRQLDDATKDKVGQELADTFVYLMRIAEVCGIDLIAAANAKIELNAQKYP
                     ADRCKGSNAKYTEYQ"
     gene            complement(2028..3215)
                     /locus_tag="VNZ84_00765"
     CDS             complement(2028..3215)
                     /locus_tag="VNZ84_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HWU65509.1"
                     /translation="MANFLNQYFATCPRGLEAILVEELKQGGARDIKATEGGASFSGE
                     LSVCYHANLHSRVATRILMQVGRGKYIKEDDFYQGALKINWPNWFDVKHSMMVKVTAV
                     RSPLKSLEFITLRVKDAICDKFRQVVGSRPNIDTREPAVRVHVYLTSDNYQLYVDTSG
                     LPLYQRGNRKNSIEAPLRENLAAGILKLTGWQPGQVLLDPMCGSGTFLLEAVMMALNI
                     APGLNRNFGFEKLKNFESDTFRKIRNAAAKAVKPVTFQSIYGSDMDLRAVRVTKQNLE
                     MAGWLEAVQLTHWEFTQVVPPAESGVLVANPPYGVRIGEDETLAALYPQMAATLKQKF
                     SGWNTYFLTNDLRLPKLMRLSPSKKTPLYNGPLECRLFEIKMVAGSNRREKADSPAVD
                     DVQ"
     gene            complement(3208..3999)
                     /locus_tag="VNZ84_00770"
     CDS             complement(3208..3999)
                     /locus_tag="VNZ84_00770"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HWU65510.1"
                     /translation="MFKFNRKIHKAVSDFEMVEVTETDGVRSMHLGSPTIQSSMKVKD
                     PYALVLAYSWGVMSSLLFKPDASKLVLVGLGGGSIAKYVWKYCPQIQQTVIELNPQVI
                     QVARSHFFVPDNDERLQIIEGDGIAYIQQHPGQCDWLMMDAFGSNGLPPDFCTQSFFD
                     DCADALTPNGLFTINLWGSDKKCDIYMQRMEQTFDDRVLMMPTGKPGNIIVFGFKPEL
                     AMPDLATLRKRAQEAMQSHHINFIDLIDRLQGANPNNGKEFTLGG"
     gene            complement(3999..5894)
                     /locus_tag="VNZ84_00775"
     CDS             complement(3999..5894)
                     /locus_tag="VNZ84_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWU65511.1"
                     /translation="MNVFYEEDGGFKVASIMSEADSSMQVEASSGKRSKIKTANVLMR
                     FEGSLSGFLESAQAEADTLDTDFLWECCGESEFSFEALAEDYYGGKPSRQQAAAIAIK
                     LHGAPMYFYRKGKGHYKAAPDDTLKAALAGMEKKRQQAELMAQMVVQLKAGTLPEGMA
                     AKLDQLLYAPDKNGMEWKALDQAAAELKKLPIQVLKDAGAIPSVHDYHLGAFLREYFA
                     HGTEFPPFSLADIPQDLPVSEVQAFSIDDSTTTEIDDALSVTILANGNTQVGIHIAAP
                     ALGVTPHEALDNEVMKRLSTVYMPGHKITMLPEVAIRPFSLDAGETKPALSLYLEVAP
                     DFTIVHQHSRLERVQIADNLRHESLEPYFNETTLEQDSGHPLWSSLVFLFRFAESLEK
                     ARGKYDPTRPQPVDYNFYVTDGIVSIINRQRGSPMDKLVAELMIAANSQWGLLLAEHG
                     VPGIYRAQNGGKVYMTTKAEGHQGLGVAQYAWCTSPLRRAVDLINQRQLISVLTLQEP
                     VYAANGDEIVGHMRNFDQTYNAYNEFQTRMERYWCLQYLIQEGVGEIDGTVWRENLVR
                     LESLPFMTKVHSLPVMKPGSKVRLAVQKVDTLLMELECKFLHLIEEVTQEAPEAGTNP
                     DELAESA"
     gene            6016..>7197
                     /locus_tag="VNZ84_00780"
     CDS             6016..>7197
                     /locus_tag="VNZ84_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HWU65512.1"
                     /translation="MKPLNFDNRFTRELPGDPITDNYTRQVSDCLWSAVQPTPVKNPQ
                     LLAYSPEVAHLLGLSAEDMHDPALIQALGGNGALDGMFTYSTRYGGHQFGHWAGQLGD
                     GRAIYLGELIHANQRYELQLKGAGETPYSRSADGRAVLRSSLREFLCSEAMHHLGVPT
                     TRALSLVTTGEPVVRDMFYDGNPQFEPGAIVCRVSPSFTRFGHFEILASRQEFSLLKR
                     LIGFTLDRDFTDWWPGTGLTLDKSNPSQAMIEAWFAEVCSRTATMIAHWMRVGFVHGV
                     MNTDNMSILGLTIDYGPYGWIDNFDPGWTPNTTDAQGRRYCFGRQPDIARWNLERLAE
                     ALSTILPDNIGLAEGIRLFDQVYIDALTTALSNKFGFDQWQDGDGDLINEIFELMQRS
                     EV"
BASE COUNT         1797 a         1945 c         1688 g         1767 t
ORIGIN      
        1 ctggctggcc aatcgcgtct cacaggtata taaagcgacc cctgtgtgat agatctccgg
       61 agcatataca ggcaacggac tatgtatcaa gacacgggtg ggctcacaaa tggcaggtgg
      121 cgcatagcca cgtgccccat ccccacgcgt aatgatgatt tttacaatcc cggaagccag
      181 ttcatcccct tctcccgtgg ccatcaagtg cttgaattcc tgcatgagga gttcagccga
      241 cgggcacaca atctggattt tgctgcaatc tgccaccagt gtctggtaat gcagcggcca
      301 atccagtaga tcaccgttga gcaggcgcat ggtacgaaaa ataccgtcac cgtaggcaaa
      361 accacggtta gtcggggcaa tgccggagac caatttacca ttgacgcaat aacggactga
      421 gctcatgtca tgcagtgaag aaagtgaagt cgaacaaggc tgaaacctta ccataaatcg
      481 gcactggcag ccctgttatc tcatgaaaat ccggccatct aacacgacac aaacggcgaa
      541 ataaaggatt ttctaaagtt tattaggaca atgccgttaa cgtgttaagg cgtaaggtat
      601 ccatattaaa tttaacgagg gatggcaatg tcagtttcca acattcaaag taacctctct
      661 gcattactga ataaggcata taacaccgat tccagcgcca cgtctgccag cgatgctatc
      721 agccagctgg ttaaagaaac aagctcatcc acagacaact cagctgcggc ttccgccagc
      781 tcccttgtga ccttgggtgg tgcagacacg accactggca cttatacagc caaaggcctg
      841 atgcagcaat tgcggcaata tcaattgagc aatgcttcct tgctgtttgg taatagtgat
      901 gactcaagtc aggacaacac cggtttgact ggtttaatgg gcacttcgtc cgatactgac
      961 ctcgcaagca tgagtgagga ctgggtgagc accattagcg agaatcctgg caaagcatcc
     1021 gtcatggtgg caagcacaca gaacgacacg ctgaaaacta tcttaggtaa taactagctt
     1081 tcacctgctt taaaaccgct tattccttga cgaaatgacc ctgcactttg tcgcccaaca
     1141 attgcagcag gtcagcagac cttaatgcca gggcaaaccc taatacgcca ctccctatga
     1201 ccacggtctc ataccgtgac acactgctat cgaccaacac aggcaaagac gctggcaagg
     1261 caatgggcgg cacggccccc acgacataac cggtggcttc cggtacttcg tcatactcag
     1321 ccattcggca ttttcgctcg ttcaactgtg ctcgcaataa agcccagtct gcgcgaccgc
     1381 caccagcaac ggctaataag caaaacccac tgttttcacc tttgaacacg acgctgcgca
     1441 ccacacattg tggatccagt ccctgcgcag aaagcaactc ttccaaatgc cggataggtt
     1501 ttttatcttc gtttaatgga atttcaatca cttgaaaatc gatctcgtgc tgtttgagca
     1561 aatccgtcac tggtgaatgg acattcgcca ccatggtcaa ccatccttta attacaaatc
     1621 actataaatg acaaatataa cttattgata ttctgtatat tttgcattac tacccttgca
     1681 tctatctgcc gggtattttt gggcattcaa ctcaatcttt gcattcgctg cagcaatcaa
     1741 gtctatgcca cagacttcag caatccgcat caggtaaaca aaggtatccg ccagttcctg
     1801 gccgactttg tcttttgtgg catcatcgag ctggcgacta tcggcttcgg tcatccattg
     1861 aaaatgctcg accacttccg ctgcttccac aatcatggcc atggcgagat ttttcggcga
     1921 atgaaactgc gcccagtcgc gctcctccac aaactgattc acacgcgtac gcaatgcatc
     1981 tattgaatct gtcatttcgc tttcctgctt aaacgattgg atacgggtta ttgaacatca
     2041 tccactgccg gagaatcagc cttttcgcgg cggttactcc ctgccaccat tttaatttca
     2101 aatagccggc attccagcgg cccattgtac aaaggtgttt tcttgctggg gctcaaacgc
     2161 atgagcttgg gcaggcgtaa gtcattagtc aggaagtagg tattccagcc agagaatttc
     2221 tgtttaagtg tcgcagccat ttgcgggtac agtgcagcca gcgtttcatc ctcaccgata
     2281 cgtacaccat agggtggatt agccaccagt acgccactct cggcgggtgg cactacctgg
     2341 gtgaattccc agtgagtcag ttgcacggcc tccagccaac ctgccatctc caggttttgc
     2401 ttagtcacac gtacagcacg caaatccatg tctgagccgt agatgctttg gaaagtcaca
     2461 ggtttgaccg ctttagctgc cgcattgcga attttgcgga aggtatctga ctcaaagttt
     2521 ttgagtttct caaaaccgaa attacggttc aggcctggcg caatattcag tgccatcatc
     2581 acggcctcaa gcaaaaaggt cccgctaccg cacatggggt caagcaggac ttgaccgggt
     2641 tgccagccag tgagttttaa aatacctgcc gccaggtttt cacgcaaagg cgcttcaata
     2701 ctgtttttac ggttaccgcg ctgataaagc ggaagacctg aagtatcgac ataaagctgg
     2761 tagttatcgc tggtgagata cacatgcacg cggacagcgg gttcacgcgt atcaatattc
     2821 gggcggctgc ccaccacttg gcggaacttg tcgcagatgg cgtctttcac acgtagcgta
     2881 ataaattcca ggcttttcaa cgggctgcgc acagcagtca ccttgaccat catgctatgt
     2941 tttacatcaa accagtttgg ccagttgatt tttaacgcac cctggtaaaa atcatcttcc
     3001 ttgatgtatt tgccacgccc aacttgcatc aggatacgtg tcgccacacg tgagtgcagg
     3061 ttggcgtgat aacacacgga caactcgccg ctaaagctgg caccaccttc agtggctttg
     3121 atgtcgcgcg cgccgccctg cttgagctct tcaacaagga tagcttccag gccacgtggg
     3181 caggttgcga aatattgatt cagaaaatta gccaccgagt gtgaactcct tgccgttatt
     3241 gggattagcc ccctgtaaac ggtcgattaa atcgataaaa ttaatgtgat gagactgcat
     3301 cgcttcttgt gcgcgcttac gtaacgttgc taggtccggc atggcaagct ccggtttaaa
     3361 accaaataca atgatattgc caggtttgcc agtcggcatc attaatacac ggtcatcaaa
     3421 cgtttgctcc atgcgctgca tgtagatatc acacttcttg tcagagcccc aaaggttaat
     3481 cgtaaacagg ccattggggg tcagtgcatc cgcgcaatca tcaaagaaag actgtgtgca
     3541 aaaatctggc ggtaagccgt tactgccaaa cgcatccatc atcagccagt cgcattgccc
     3601 tggatgctgc tggatataag caataccgtc accttcgata atctgtaaac gctcatcatt
     3661 gtcaggcaca aaaaaatgac tacgtgccac ttgaatcact tgcggattca gttcaatcac
     3721 agtttgctgg atctgcggac aatatttcca cacatatttg gcaatcgagc caccccccaa
     3781 accaaccaat accaacttgc tggcatctgg cttaaacaac aggctactca tgacgcccca
     3841 tgaataagcc aacaccaggg catagggatc tttgactttc atgctgcttt gtatggtggg
     3901 agaacccagg tgcatggagc gtacgccatc agtctcggtc acctcaacca tttcaaagtc
     3961 agacacagcc ttgtggattt ttcggttgaa cttaaacatt atgcactttc tgccagttcg
     4021 tctggattgg tgccggcttc aggcgcctct tgtgtcactt cttcaatcag gtgcaagaac
     4081 ttgcactcca gttccatgag caaggtatct actttctgca cggccaaccg cactttgctg
     4141 ccaggcttca tgaccggcaa gctgtgcacc ttggtcataa agggcaaact ttcaagacgc
     4201 accaggtttt cacgccatac cgtgccatca atctcgccta ccccttcctg gatcaggtat
     4261 tgcaaacacc agtagcgctc catgcgggtc tggaattcat tataggcgtt atacgtctgg
     4321 tcaaaattac gcatatggcc gacgatttca tcgccattag ccgcatatac gggttcttgt
     4381 agtgttagca cgctaatcaa ttgtcgctgg ttaattaaat ctacggccct gcgcaatggg
     4441 gaagtacacc aggcatattg cgccacgccc agcccttgat gcccttcggc tttggttgtc
     4501 atatacacct tgccaccatt ttgtgcacgg taaatgccgg gaacgccatg ctcggccagc
     4561 aacaagcccc actggctatt ggcggcaatc atcagctcgg ctaccagttt atccatgggc
     4621 gagccacgct gacggttgat aatgctgaca atcccatccg tgacatagaa gttgtaatcc
     4681 actggctgtg gccgcgtcgg gtcatactta ccccgcgcct tttccaggct ctcggcaaaa
     4741 cggaacagga acaccagtga tgaccataag ggatgtccgc tatcttgctc cagggtggtc
     4801 tcgttaaaat aaggctccag tgactcatgg cgcagattat cagcaatctg cacgcgctcc
     4861 agacggctat gctgatgcac gatagtgaaa tcaggcgcga cttccaggta gagcgacaag
     4921 gcaggcttgg tttcccccgc atccaggctg aatggccgga tggcgacctc tggcaacatg
     4981 gtaattttat gcccgggcat atacacggtc gacaggcgct tcatcacctc attatccaac
     5041 gcctcatgcg gtgtcacacc caatgctggc gcagcaatat gaatacccac ctgtgtattg
     5101 ccattggcta agatggtcac agataaggca tcatcaatct cggtggttgt actgtcatca
     5161 atactaaatg cctgcacctc agacactggc agatcctgtg gaatatccgc caatgaaaat
     5221 ggcgggaatt cagtcccatg ggcaaaatac tcgcgtaaaa aagcgcccag gtgataatca
     5281 tgtaccgaag gaatcgcgcc agcatccttt aatacctgga taggtaattt cttaagctcg
     5341 gcggctgcct ggtccaaggc tttccactcc atgccatttt tgtcaggcgc atacaataac
     5401 tggtcaagtt tggcagccat accctctggt agcgtgcccg ctttaagttg cacaaccatt
     5461 tgcgccatga gctcggcctg ctggcgtttt ttttccatac ccgccagtgc tgccttcaag
     5521 gtgtcatctg gcgcggcttt ataatgcccc ttacccttgc ggtaaaaata catgggcgca
     5581 ccgtgtaatt taatggcaat cgccgcggcc tgttgccggc taggcttacc gccataataa
     5641 tcttcagcca gcgcttcaaa gctgaattca gactcgccac aacactccca caagaagtct
     5701 gtatccaatg tgtcggcctc tgcctgtgcg gattcaagaa aaccggacaa gctgccctca
     5761 aaccgcatca atacgttagc cgttttgatt ttgctgcgtt tgccgctgct ggcttcgacc
     5821 tgcatgctgc tgtcggcttc agacatgata gaagcgacct tgaagccacc gtcttcttcg
     5881 taaaaaacat tcatgtggga aattaaatgt gtggaaatca cactgcgagt attcaaaacg
     5941 ctattttacc cgagaagcac agactttttt ggcaagcact gaactttagc cgaggataat
     6001 gactaaaata agaggatgaa gcctttaaac tttgacaacc gatttacccg cgaactgccg
     6061 ggcgacccca tcacagacaa ctatacacgc caggtcagtg attgcctgtg gtcagcggtt
     6121 cagccaacac cggtcaaaaa cccgcaactg ctggcatata gccccgaagt cgctcacctg
     6181 ctcggcttat ctgccgagga catgcacgac ccggcgctga tccaggcgct gggaggcaat
     6241 ggcgcattgg acggcatgtt tacttattcg acccgttacg gcggtcacca gttcggacat
     6301 tgggcgggcc aattaggcga tggccgggcc atttacctgg gtgaattgat ccatgccaat
     6361 cagcgctatg aattgcagct gaaaggggcc ggcgaaaccc cctactcccg ttcagccgat
     6421 ggccgcgcgg ttttacgttc cagcctgcgt gaatttttat gctctgaagc tatgcatcac
     6481 ctgggcgtgc caacgacgcg tgccttaagc ctggtcacga caggagagcc tgtggtacgg
     6541 gatatgtttt acgacggcaa cccgcagttt gaaccaggtg caattgtatg tcgcgtatcc
     6601 ccctcgttta cgcgctttgg ccattttgaa attttggcca gcaggcaaga attttccctg
     6661 ttaaagcgct tgatcggttt taccctggac cgtgatttta ctgactggtg gcctggcact
     6721 ggcttgacgc tagacaaaag caacccatca caagccatga ttgaagcctg gtttgccgaa
     6781 gtatgctcac gtaccgccac catgatcgca cactggatgc gcgtcggctt tgtgcatggc
     6841 gtgatgaata cagacaatat gtctatcctt ggcctgacca ttgattacgg cccctatggc
     6901 tggatagata actttgaccc gggctggaca ccaaacacga ctgacgcaca aggccgacgc
     6961 tattgttttg gccgccagcc agatattgcg cgatggaacc tggagcgcct ggccgaagcc
     7021 ttgagtacga tattgcctga caatattggc ctggccgaag gcatacgcct gtttgaccaa
     7081 gtctatattg atgcgctgac cacggcgcta tccaataaat ttggctttga ccagtggcaa
     7141 gatggcgatg gagatctgat caacgagatt tttgagctca tgcaacgctc agaagta
//
LOCUS       DATMSK010000024         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_236900,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000024 DATMSK010000000
VERSION     DATMSK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236900"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VNZ84_00785"
     CDS             <1..961
                     /locus_tag="VNZ84_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWU65513.1"
                     /translation="NQAAALDRVNTYLAANPKNAEALFMRGVILVELGKRDDAIKAFT
                     ELTEKFPNLPEPYNNLAVLYADQGQYDKARKALESAIKTHPSYATAHENLGDIYARLA
                     SEAYDKALKLDTSNSRAQNKLAMITDLFGGKRTTAARTVTPAPSVPATQSPATTSPAN
                     PAVNNPPTTQPQPTATPPVATPPVAEAKKPAAVEPKPAEEKPAATQERAVLDAVNAWA
                     DAWSAQNVERYLDSYAKDFKTPGGESRKQWESTRRDRVSRPSKISVKLNNLSVKIESD
                     TAAKVTFRQVYKATGLDANSNKTLNMVKEGSRWVIQQERVGKF"
     gene            1064..2296
                     /locus_tag="VNZ84_00790"
     CDS             1064..2296
                     /locus_tag="VNZ84_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWU65514.1"
                     /translation="MRSLRKYCYIAVVSAALLPWNATAVNHEALTARLFVQNKSTSPV
                     ERMLVQTLQSIKEGRLQQALDYVDQLLAISPNFALAHLIHGDLLSAKARILNNFGDAN
                     VADTTRIQDFKEEAEIRIRNYFDRNRGLTQPNMLVELSEKQSHVLFVDTARSRLYVYE
                     KGEGDVLKYTADYYVTIGKNGIGKKDQGDKRTPIGVYFASRKLNRPLPDLYGDAAYPL
                     NYPNEIDSYERKTGSGIWIHGTPRDTYSRPPRASDGCVVLSNPDMKALQDILQAGNTP
                     VVIGVNFQWVNPESLRKQDRAKADLRSAIERWRKDWASQNTDAYLSHYTNDFFYSEGG
                     LSKWASYKRQIQATKPKVSINLSEVSMFTYPNSTRNMVVVDFEQEYLSPALRNVMKKR
                     QYWVQENGEWKILYEGSA"
     gene            2329..2865
                     /locus_tag="VNZ84_00795"
     CDS             2329..2865
                     /locus_tag="VNZ84_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWU65515.1"
                     /translation="MLITVGVANAATQVLFETNQGNFTVEVYADKAPKTVENFLQYVK
                     DGFYSNTIFHRVIGRFMIQGGGFDRELNEKPTRAPIVNESNNGLLNATGTLAMARTPD
                     PNSATAQFFVNVADNQFLDYTSPEPDRIGYCVFGKVISGMDVVFKISNLPTGNQRGFS
                     DVPIRTVIIKRASIINGK"
BASE COUNT          831 a          766 c          695 g          633 t
ORIGIN      
        1 gaatcaggca gcagcgctag accgcgtaaa cacttacctg gcggccaatc ctaaaaatgc
       61 cgaagccctg tttatgcgtg gcgtcattct ggtggaactg ggcaagcgcg atgatgccat
      121 caaggcattt acagagctca ccgagaagtt cccaaacttg ccagagcctt ataacaacct
      181 ggcggtactc tatgctgacc aaggccagta cgacaaagcc cgaaaagccc tggaatcagc
      241 aatcaagact cacccaagct atgcaactgc ccatgaaaac ctgggggaca tttatgcgcg
      301 actagcttca gaagcgtatg acaaggcact caagttagac acctccaaca gtcgcgccca
      361 aaacaaactg gccatgatca ccgatttatt tggtggcaaa cgtactacgg cagcgcgcac
      421 tgtcacgcca gcgccttcag taccggctac gcaatcaccg gcaaccacaa gcccggcaaa
      481 tcctgctgta aacaatccac caacgacaca gccgcagcca acagccacac ctccggttgc
      541 aacgccgcct gtggctgaag ccaagaaacc tgcagcagtg gagcctaaac cagcagaaga
      601 aaaacctgcc gccactcagg agcgtgccgt actggatgcg gtcaacgctt gggcagacgc
      661 atggtcggcc caaaatgtgg aacgttatct ggatagttat gccaaagact tcaaaacacc
      721 aggtggcgaa tcgcgcaagc aatgggagtc caccagacgt gaccgtgtca gccgcccaag
      781 caaaatatca gtcaagctga ataacctctc ggtaaaaatc gagagtgata cagccgcaaa
      841 agtcactttc cgccaagtgt ataaagccac tggcctggat gcgaattcga ataaaaccct
      901 gaacatggtc aaagaaggaa gccgctgggt cattcagcaa gaacgcgtgg gcaagttcta
      961 agcccattgc aagttgagcg acacctgtaa aagcattaaa taagcagcga atttggctgt
     1021 ttaagcacgt aagatttgat ttaaacaaat gtataatatc ggcatgcgta gtttaagaaa
     1081 gtactgctat attgcggttg tcagtgctgc acttttgcct tggaatgcaa ccgcagttaa
     1141 ccacgaggcg ttaactgcgc gtttgttcgt ccaaaacaag agcaccagcc cggttgaacg
     1201 catgctggtg caaaccctgc aatccatcaa agagggtcgt ttgcaacagg cattggacta
     1261 tgtcgaccaa ctgctggcca tttccccaaa ctttgcgctt gcccacctga ttcatggcga
     1321 tttactctcc gccaaagcca ggatattaaa caatttcggt gacgctaatg tcgccgatac
     1381 cacccgcatc caggatttca aagaagaggc agaaatccgt atccgcaact actttgaccg
     1441 caaccgcggc ttaacccagc ccaacatgct ggttgaactg tcagaaaagc aaagccacgt
     1501 cctttttgtt gacactgccc gctcccgtct ttatgtttat gaaaaaggcg aaggggacgt
     1561 attgaagtat accgctgact actatgtcac cattggtaaa aatgggatag gtaaaaaaga
     1621 ccagggggac aaacgtaccc ctataggcgt ctatttcgcc tcacgcaaac tcaatcgccc
     1681 gctgccagat ttgtatggcg atgctgctta cccgctcaac tatcccaatg aaatcgacag
     1741 ctatgagcgc aaaaccggct ccggcatctg gatacacggc acgccacggg atacatacag
     1801 ccgtccgccg cgcgccagtg atggttgcgt cgtgctgtcc aacccggaca tgaaggcgct
     1861 gcaagacata ctgcaggctg gcaatacgcc tgtggtgatt ggtgtcaatt tccaatgggt
     1921 caatccagag tcgctgcgta agcaggaccg tgcgaaagcg gatttacggt ctgcaataga
     1981 gcgctggcgc aaagactggg cctctcaaaa taccgatgcc tacctgtctc actacaccaa
     2041 cgatttcttt tactcagaag gcggcctctc caaatgggcg agctataaac gccagattca
     2101 ggccaccaag cccaaggttt ccatcaacct ttctgaagtc agcatgttca cttatccgaa
     2161 ctctacgaga aacatggtgg tcgtagattt tgagcaggaa tacttgagcc ctgccctgcg
     2221 taatgtcatg aaaaaacggc agtactgggt gcaggaaaat ggtgaatgga aaattttgta
     2281 cgaaggatca gcttgatgcg aatgttgtct tggatagtag gttgctgcat gttaatcaca
     2341 gtaggcgttg ccaatgcggc gacacaggtg ctatttgaaa ccaaccaggg taactttacc
     2401 gtggaggtgt atgccgacaa agcgccaaaa acagtggaaa actttttgca gtatgtgaag
     2461 gatggctttt atagcaacac cattttccac cgcgtgattg gtcgctttat gattcagggc
     2521 ggtggttttg accgtgaact taatgaaaaa cctacccgcg cacctatcgt gaatgaatcc
     2581 aacaatggct tgttgaatgc gacaggaacg cttgccatgg caagaacgcc agatcccaac
     2641 tcagccacgg cacaattttt tgtcaatgtg gcagacaacc agttcctcga ttacaccagc
     2701 ccggaacctg acagaatcgg ctactgtgta tttggcaaag ttatctccgg catggatgtc
     2761 gtatttaaaa tcagcaactt gcctacaggc aaccagcgcg gtttttcaga tgtaccaatc
     2821 cgcaccgtga tcatcaagcg tgccagcatt attaacggaa aataatttcc aaccctcaat
     2881 acaacggaga atattttggt aaaactgact accaattttg gcgat
//
LOCUS       DATMSK010000025         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_49355,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000025 DATMSK010000000
VERSION     DATMSK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49355"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="VNZ84_00800"
     CDS             <1..118
                     /locus_tag="VNZ84_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HWU65516.1"
                     /translation="GYGLGIRWRSPIGPVGADIAYGEETKEYRLHFNLGVNF"
     gene            127..3414
                     /locus_tag="VNZ84_00805"
     CDS             127..3414
                     /locus_tag="VNZ84_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HWU65517.1"
                     /translation="MKTLKQLLNILLTSVLCLSLIITPQLVWAANVMLVTSLKEFETV
                     LGNASLKVEGIDSSMRLGVTPTGELTVSHFRAKQVTLRFKQPTADAPSPSPKSEPAPL
                     PARIPVPLPFYLLSGQIDHLTIIQGESISHITQIHFDLDADNQALQFRIAEAHSPWGK
                     VSTHFQMQNATPFALSGWLDVQQTRGELPYHLRTELHGDLTRIEIAANHHYQPQARPF
                     AIVPANGQTEDMLQINAVIGLDQTRQSHLLCHLHQFQAKHIHPQLAGHMDLKIVADGS
                     LTEQGEIVASVDAGDSRLQGQQLTVRGTASLQGAQLLNMDLLAQLDKNTLTMHTDQDA
                     SSHMGSTLTWQANLSNLAQLMPGFSGQIKGTGKVQQSTEGFATDYQLSGQQLQLPQGL
                     ILTAFEAEGQASNRAQASLENHIKLRGLSQRNAQGTDSPPIDAELNLTGTLATHQLSL
                     QIKDSDPLLQRNLKLALEGNWQNDRWQAQLTQLQDDDGKVFQLVNPASLSWQTGQGFQ
                     LKNLVIHALTGRLQLDHLHYRPPQAANPGTQQSAEKVQFSSQGSLKDFPVQAILAWLI
                     PEHTEALPADHLRVSGQWQLALDEQLNGSLRLERASGDWQTYDNNQMHWQGLGIHTLF
                     ADINAKNNRVSVQSSIHADHAIDLQFAADTIMTPTQNGIVIQRSAPLSAQLKANIGQL
                     AWLSKLMPELQPAGQLSIDAQANGIISQPQLQGDIQGKALALHMPSQGVWLEQGHLDA
                     KLAGDQITVDQLHFAGKSGELNGKGQLTFKPDHWQLEAMMQLNKLQALSRVDRWVQVS
                     GDTRVIMTSEQATLSGKLKIHKGLFELPKADKPKLDEDVVIESPQASKPEPASRFAFK
                     DFSLDFGDKPLVLPFKESEQFMLRGQGLNGALSGKMQLNGMLDDLAAAGTLEITGTYI
                     AYGQSLNIETGRLIFSGKLPNIGLDILATRQVESTKVGIQINGSLQTPQLKLVSTPET
                     SNENKVSLLVLGQPMSQVGSSDMAMLSVAAGALLSQGDSVSLQTKIAQAVGLDSIDVR
                     GTGPTNYAVSVGKRINRNLVVGYEKSIVGLLNVGKLTYQLTKRISIETRTGSDNALDV
                     FYGFSFN"
     gene            3431..>4017
                     /locus_tag="VNZ84_00810"
     CDS             3431..>4017
                     /locus_tag="VNZ84_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWU65518.1"
                     /translation="MTTDKINPRLAVLIDADNTFNVIPIIDALFEEISKFGDASIRRI
                     YGDWTQHQLSRWKEVLPKHAIQPMQQYANTKGKNATDSALIIDAMDLLYTAPLDGVCI
                     VSSDSDFTRLAIRFRESGKQVYGFGEAKTPESLRAACNQFVFIEILSQDKKISAEETD
                     TFTNNDKILTEKPDTPGKPSLAIPEIVPLSPVVKK"
BASE COUNT         1141 a         1047 c          944 g          885 t
ORIGIN      
        1 cggctatggg ctggggatac gctggcgcag tcctatcggt cctgtaggtg ccgatatcgc
       61 ttatggcgag gaaaccaagg aataccgttt gcacttcaac ctgggcgtaa atttctgatg
      121 ccactcgtga aaactttaaa gcaactactc aacatactgt tgaccagtgt gctgtgttta
      181 tcactgatca tcacaccgca attggtatgg gcggccaatg tcatgctagt gaccagcctc
      241 aaggaatttg aaaccgtcct gggcaatgcc agcctgaaag tagaaggcat agactccagc
      301 atgcgtttgg gcgtcacacc cacgggggag cttactgtct cgcatttcag ggccaagcaa
      361 gtcaccttgc gttttaagca gcctacagcg gatgcccctt caccctctcc gaaatctgaa
      421 ccagcccctt tacctgcccg tattcctgtt ccattacctt tttatttgct atccggacag
      481 atagaccatc tcacaattat tcaaggcgaa agtatctcgc acatcacaca aattcacttt
      541 gatctggacg ctgataacca ggccttgcaa ttccgcattg ccgaagcaca tagcccgtgg
      601 ggcaaggtca gtacgcattt ccagatgcag aatgcgactc cttttgcgct ctctggctgg
      661 ctggatgtac aacaaacacg cggagaactc ccctaccatt tgcgtaccga attgcatggt
      721 gacctgacac gtattgaaat agcggccaac catcattatc aaccacaagc tagacctttc
      781 gcgattgtgc cggcaaacgg gcagacggaa gatatgctgc aaatcaacgc tgtcattggc
      841 ctggatcaaa cacggcaaag ccacttgtta tgccatctgc accagtttca agctaagcat
      901 atccacccac aactggcggg tcacatggat ttaaaaatcg tcgctgatgg cagcctgacc
      961 gaacaaggcg agattgtcgc ctctgtagat gcaggagata gccgtttgca aggacaacag
     1021 ttgaccgttc gcggcactgc cagccttcaa ggtgcacaac tgctaaacat ggatctgttg
     1081 gcacaattgg ataaaaatac actgaccatg cacacagatc aggatgcctc gtcgcatatg
     1141 ggcagtacac tgacatggca agccaacctg agtaatctgg cacaattgat gccaggattc
     1201 tccgggcaga tcaaaggcac aggcaaggtg cagcaatcta cagaggggtt tgccacagat
     1261 taccagctgt ccgggcaaca attacaactc ccacaagggt tgatcctgac cgcctttgag
     1321 gctgagggac aagcctccaa ccgtgcacag gcctcattgg aaaaccacat caagctacgt
     1381 ggtttgtcac agagaaatgc tcaagggact gacagccccc cgattgatgc cgaattaaat
     1441 ctgacaggca cattggcaac gcatcaattg tcgctccaaa tcaaggacag tgaccctttg
     1501 ttgcaacgca acctaaaatt ggcgctggaa ggcaactggc agaacgaccg ctggcaggca
     1561 caactgacgc aattacaaga tgatgatggc aaggtattcc agttggtaaa tcctgccagc
     1621 ctgagctggc aaactggaca aggtttccag ctgaaaaacc tggtcattca tgcgctcaca
     1681 ggccgattac aactcgatca cttgcattac cgcccaccac aagcggcgaa tcctggcaca
     1741 cagcaatctg ccgaaaaagt acagttcagc agccaaggct cactgaaaga cttcccagtc
     1801 caggcaatcc tggcatggct gatacctgag catacagaag ccctgcctgc agaccatttg
     1861 cgcgtatcgg ggcaatggca actggcgctg gatgaacaac ttaatggaag cctacgcttg
     1921 gaacgtgcct caggagactg gcaaacctac gacaacaacc agatgcattg gcaagggcta
     1981 ggcatacaca ccttattcgc tgatatcaac gccaaaaaca atcgtgtcag tgttcaaagc
     2041 agcattcatg cagatcacgc catcgacctt caattcgctg ctgacacgat catgacaccc
     2101 acgcaaaatg gtattgtgat tcaacgctca gcgccgctgt cagcacaact caaagccaat
     2161 attggtcaat tagcctggtt gtccaaactc atgcctgaac ttcagccagc cggccagtta
     2221 agcatagatg cccaggccaa tggcataatc tcccagccac agctgcaagg tgatatacag
     2281 ggtaaagcat tggccctgca tatgccgtcc caaggcgtct ggctggaaca aggtcacctg
     2341 gatgccaagt tggcgggtga tcagatcacg gttgaccagc tccactttgc aggaaaaagt
     2401 ggagagttaa atggcaaagg tcaactcaca ttcaagccag atcactggca actagaggcc
     2461 atgatgcaat tgaacaagct acaggcgctt tccagagtag atcgctgggt acaagtcagt
     2521 ggtgacaccc gcgtgatcat gaccagtgag caagcgacat taagcggcaa gctgaaaatc
     2581 cataaagggc tgttcgagct accaaaagca gacaaaccaa agctggatga agacgtggtg
     2641 attgaatcgc cacaagccag caaacctgaa cctgcttcca ggttcgcatt taaagacttc
     2701 tcactcgact ttggtgacaa accactggta ttgccgttta aagagtcgga gcaatttatg
     2761 ttgcgtggtc aaggcttgaa cggcgcactc agtggcaaga tgcagttgaa tggcatgctc
     2821 gatgacctgg ccgcagcagg cacactggaa atcacgggca cttatatcgc ctacggccaa
     2881 tcattgaata tcgaaaccgg ccgcctcatc ttcagcggca aactgcccaa tattggcctg
     2941 gatatcctgg cgacaaggca ggttgaaagc accaaagtcg gcattcaaat caatggcagc
     3001 ctccaaacgc cccagttaaa actggtatcg acaccggaga ccagcaatga aaacaaagtc
     3061 tcgctattag tattggggca gcccatgtcg caagtgggaa gtagtgacat ggccatgctt
     3121 tccgtcgcag cgggggcatt gctctcacaa ggggattctg tttccttgca aaccaaaatt
     3181 gcccaggctg ttggcctcga cagcatagat gttcgcggca ccggaccaac caactatgcc
     3241 gtcagtgtcg gtaagcgtat taaccgcaat ctggtcgttg gctacgaaaa aagcattgtc
     3301 ggcctgttaa atgttggtaa actcacttac caactgacta aacgtatttc tatcgaaacc
     3361 aggaccgggt ctgataacgc actggatgtg ttttatggct tcagctttaa ctaattgagt
     3421 gcttgaacgt atgaccacag acaaaatcaa cccaagatta gccgtactta tcgatgcgga
     3481 taatacattc aacgtaattc ccatcatcga cgcactgttt gaagagattt ccaaattcgg
     3541 cgatgctagc atccgccgta tctatgggga ctggactcaa catcagttaa gtcgctggaa
     3601 agaagtacta ccaaagcacg ctatacaacc tatgcagcaa tatgccaata ccaagggcaa
     3661 gaacgcaacc gacagtgcgc taatcattga tgcaatggat ttactgtata ccgcaccact
     3721 ggatggcgta tgtatcgttt caagtgatag tgacttcacc agattagcga tccgctttcg
     3781 tgagtctggt aaacaggttt atggatttgg tgaagctaaa actcctgaat cattgcgtgc
     3841 ggcctgtaac caatttgtat ttattgagat tttgtcacaa gacaaaaaaa tatctgcaga
     3901 agaaactgac accttcacaa ataacgataa aatcctcacg gaaaaacctg atactccagg
     3961 aaaaccgtca cttgctattc ctgaaatagt gccactcagt ccagtggtca agaaaag
//
LOCUS       DATMSK010000026        11984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_72587,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000026 DATMSK010000000
VERSION     DATMSK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11984
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72587"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..236)
                     /gene="pqqA"
                     /locus_tag="VNZ84_00815"
     CDS             complement(165..236)
                     /gene="pqqA"
                     /locus_tag="VNZ84_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HWU65519.1"
                     /translation="MWTKPAATEMRFGFEVTMYVMNK"
     gene            complement(435..1073)
                     /locus_tag="VNZ84_00820"
     CDS             complement(435..1073)
                     /locus_tag="VNZ84_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU65520.1"
                     /translation="MIVKVVLVDDHTVVRSGLRRLLESHKSIEIVAEADTGEMAYQVY
                     GEVLPDVILMDISMPGMGGLEAAKRILQRYPQAKIVIFSMHEAVSFAAQALKTGVKGY
                     VTKTGVADDLVQAVLDVSKGRTFLSQDVAQKVALQTLIGESNPLQQLTSREFEVFRLL
                     AEGKRVEDVADMLKISQKTVANYYTLIKQKLSVNSPVEMVRLAMKHGLIDTE"
     gene            complement(1070..2446)
                     /locus_tag="VNZ84_00825"
     CDS             complement(1070..2446)
                     /locus_tag="VNZ84_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU65521.1"
                     /translation="MKSFSLQFKLNLIITLLLLGLLLTWAVSVIQNAREDVLAEVDST
                     TNLVMHLLDAQIAHYMTDFGWLSRSGEGEVSVFRLEELTNIRHLKVDFFDVSGRLRES
                     NRSTRVEHAGEDPPIWFVRLLDISSVLNEPKRKNIILNGRLIGELVITPDPSFELREI
                     WDDTSGLLAIVGLFGLSVLLFVYWTVRATFKPVKQIIHGLNQIEHGEYQSRLPGFEQV
                     ELSEISDKFNKMASTLEQSIRNNHRLTQQIIHLQEEERKKLARDLHDEIGQYLTAVHI
                     DASAMVAAKKLTVARESAKAIAQITRQMMFTIQDLLARLRPRVLDELGLSLALAELIH
                     HWHDRNRSIMVVNEISNHLTQPDESVAVTVYRILQECLTNISKHAAANRVTIAVSDKA
                     GGLYLLVEDDGRGFDPAVPSRGYGLAGMQERVEGLMGTMQITSAPGQGTRIEVHFQNI
                     GKFQDTAP"
     gene            complement(2614..3576)
                     /locus_tag="VNZ84_00830"
     CDS             complement(2614..3576)
                     /locus_tag="VNZ84_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWU65522.1"
                     /translation="MQTKLSNLILAGALASFCSNVFSQPLAYVPNEKDGTVTVIDTST
                     DQVINTLPKKGKLGKKVQAAAIHPSKQKLYVVVRDKNAVSVVDTQLGKQTALIKVGDE
                     PEGIDISPDGKWLAACLEEENAVSLVDLQTNKLKHTIKTQGKNPEHCVFSPDQQWLLA
                     SNEESNNVDVINLNTLKSEHLIASTKHPRGVGFSPDGKWAYVANEAASLLEIVSTADW
                     KVQANIKVGLRSNGVKVNADGSRIYVSNGGDHNLSVIDTASRQIIATISVGQRPWNMA
                     LTPDGKKLYVANGRSNNVSVIDTQTNTKLKDIPVGALPWGVVIR"
     gene            complement(3756..4706)
                     /locus_tag="VNZ84_00835"
     CDS             complement(3756..4706)
                     /locus_tag="VNZ84_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWU65523.1"
                     /translation="MIKTLCALLGMSLGLCVQAADYAYITNQGEDSVSVVDLQLQQVV
                     NTIKVGKAPVGVAVDKVNQKVYVSNVEGRSLSVIDMQRQQLANTIALPIAPVGITVSH
                     RGDRAYVADWYHDTLLVVATDDGRIIAQVKVGNAPAGMQLSLDDATLYVANRDSNDVS
                     VVDTRQNKERRRIPVGQHPFGLSLSPSGRQLFVVNVYSNSVSVVDTHDDKVVTTLAVG
                     DHPYCATTDHRGQRLFVTNTGEDSVTVIDLASRRTTGKIRVPGTPEGIAYDAVRQQIL
                     VASWMDNELAVIDDSSQVLKSAIKVGSQPRAFGQLIGHAP"
     gene            complement(4873..5265)
                     /locus_tag="VNZ84_00840"
     CDS             complement(4873..5265)
                     /locus_tag="VNZ84_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWU65524.1"
                     /translation="MHSNPIPQLPVAIDDQQQFNQHVAGHDFVVLLFTAKWCDPCQAF
                     YPAFNEVASQYPSISFAIADIDVATDLAANFQVAQVPALMVVRERVIIDRVTGAMHAH
                     ELQHHLQMWQAFDMTAINEHFSQSTSIA"
     gene            complement(5269..5640)
                     /locus_tag="VNZ84_00845"
     CDS             complement(5269..5640)
                     /locus_tag="VNZ84_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65525.1"
                     /translation="MRVSLPFLFLVCLGVHPLCQAEGAVLELVRLVETDDSLCLAHGG
                     KMVSLQLTEAALPVQEGPVEVWVDRWFMQVQTADHTRHVLTSDKPETELGCSHSIAGP
                     QHWTLATIKRLPVAGAPYRKE"
     gene            complement(5650..6243)
                     /locus_tag="VNZ84_00850"
     CDS             complement(5650..6243)
                     /locus_tag="VNZ84_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWU65526.1"
                     /translation="MKWSLLLAGLLLSANVWAHGPTPQKTDESIQVGASAESVWKRIS
                     APCALAQWHPQVTACEAVGDKQQKLTLKNGKHLLQEIDEVAAADMTVSYRLSGDIDLE
                     ALPVSSLNGKIKVSADGSNAKVVWTARYYRAFTGNEPPAGQDDESAKSAVDAFVKEGL
                     QGLKDGRNAAANSSGWRHKVCVAMRPVFKSLGISVCA"
     gene            complement(6266..7366)
                     /locus_tag="VNZ84_00855"
     CDS             complement(6266..7366)
                     /locus_tag="VNZ84_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HWU65527.1"
                     /translation="MAKRFLGPLAARLRRWMPRWLMAGKRQADWRQRCLFLMLVLLTL
                     ILFKPHWVLPNRVYDWYFVVDITQSMNVSDYQLSGKSVSRLQMAKQSIRKTLSALPCG
                     SQVALGMFTERNVVTVVKPVEVCSHFAALDQTVASMDWRMAWAADSFIAHGVYNALEQ
                     TPNLGKHMRLMFFTDGQQAPPANPKYMPVYAGKPGEVQGYLVGMGKPTPSKIPKLDEK
                     NAVSGYWEQEEVQRFGNFGMAETLSVLAMEQGQHDRNAGHGPGNTLLENAHLSALDAQ
                     NLRRLGDATGLHVVRLDDVAQGSSWATGLGMTAWRWADTDLRPWLAWPVGVAWMVYLA
                     LTFAGRGWLGMFGRAWRGARVRLIKPSGKPSL"
     gene            complement(7366..7968)
                     /locus_tag="VNZ84_00860"
     CDS             complement(7366..7968)
                     /locus_tag="VNZ84_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65528.1"
                     /translation="MNAKLAGVMRRGLLWASVIIVISLVMLARQLIELYQAQIYRSSL
                     EPHVLITQPMLGANQTPQALFANAVFYDNQLKPEQALTQYAAVASRVGEDDPLRKQAY
                     FNSGNVYMRTATRLLEQRGLPAWDEAGPLVALAKESYQKALRIAPEWSEVKYNYHLAL
                     RLAPTTHGMSGPQQYEDEQIKAEQEPSGWPAMPGNPRGMP"
     gene            complement(7965..8996)
                     /locus_tag="VNZ84_00865"
     CDS             complement(7965..8996)
                     /locus_tag="VNZ84_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HWU65529.1"
                     /translation="MWHDGTQQLLLATGYGEHWGLTQLWPLLLLPLAMLPWLLRGQQS
                     FAYPALQGLPEDPLSAWLDRLWKMALSLLMLSLSLGLAGPYLKSQMIEKVGRGAHVMI
                     VLDRSASMNEPFVKKGDAQARVPKMEVARNVLKTLVAQGHDDLMGMVTFSTSPILAAP
                     LGNDRAFVQAALDATEAGGMGFTAVARGLGMALDYFADQPVTGARAMVLVSDGGAHLD
                     GKTQDMLRAMFMRQQASLYWIYLRSESGVSLKQSLAEGENIDAYPEYALHEYFQTLNV
                     PYRIYEAENPEAVMAAVRDISTLKNKPTRYLEPVARQDLSHYAYFLAWLAAVLLVAMF
                     GLELKQWQS"
     gene            complement(8999..9925)
                     /locus_tag="VNZ84_00870"
     CDS             complement(8999..9925)
                     /locus_tag="VNZ84_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65530.1"
                     /translation="MNAGLHTHQNTTCRVWLAALVLLQLGSGQACAAVTTEGMQVRAE
                     SATRETDYTLGSIARQHLTLHVPLGFELDPGSLPLPAQNEAIELRDAHWTTTDSGNER
                     LIQLEIDWQIFAAGDTVKTIPLKNLHLEFKRDQQRLMVDVPADKVIVSSLLPARIDAE
                     HVKLYPDAPLPEWSLSNQLWKLAGWLCLLGLATLYLCWYMGWIQLPQEKRMPFRQAWR
                     AIRALPEGHASSLQAMCLISRAMDQFAGYAITTENLPRLLTMHPALDTYSESLRQFYQ
                     AVQHSFFAGQQASLSLRELKKLAKQLSQVEIS"
     gene            complement(9922..10854)
                     /locus_tag="VNZ84_00875"
     CDS             complement(9922..10854)
                     /locus_tag="VNZ84_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWU65531.1"
                     /translation="MGLSSVSADLSSPAPEVHATFEPRLFFYNINWRSRSYHAGATLT
                     GHHGNGSDFAGFAPLLSCPDPKRIDIRASVRSTPRQWLVRTYLERAAIHVYAVVDLSS
                     SMRFQGHDQLRQQVTEFVSSLAWSATRQGDAFGMRGAHDTPQAVWQVMPTFQPATAQG
                     VAQKITRYWQDTAAGEKGHGAAGIPAAIESLGARRALVFLLSDFYWPDALMQQVLKAA
                     QSHDVVPVVLRDRAATTGLPAFGWARLRDMETGEERSWMLRRRLHQQIADYGQQQAQA
                     LDAQFLKAGMRAPLWLRPGWRAEDISRHLMETMA"
     gene            complement(10878..11897)
                     /locus_tag="VNZ84_00880"
     CDS             complement(10878..11897)
                     /locus_tag="VNZ84_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWU65532.1"
                     /translation="MENKVDLPAWRERALAFEQALNSVVVGMPEQIRALAIAIFSRGH
                     VLLEGDVGVGKTTLLRAAAHLLGGGYQRIEGAIDLMPSDFLYHAYIDEQGQAAVKPGP
                     LLQHDEALSVFFFNEINRARPQAHALFLRLMAERSVNAFNREYHFPHLTVFADRNRLE
                     REETFEIPAAARDRFLMEISIKAPDARDLQRALMFDTRFYDVDRLVDGLPVGLTPYAE
                     LNQVAEAVQASVRAEVPLQDYAVDLWRTLKDPQAAGIQLPDVDMSRLLAGGASPRGMA
                     MLMRSGRTRAWLEGRDYLTPDDIRAVWPFVVTHRVFFNGAYALQHERLAPMLLQAVLD
                     KVSAP"
BASE COUNT         2767 a         3489 c         2991 g         2737 t
ORIGIN      
        1 tctagtgatt gtctggtttt ttcaatttcg ccagcaattc ttgcgcgctt atcgatcagc
       61 caatttaatg aaggtggaat tctgttcgcc ataacagtat tccaccatat tcctttctct
      121 tatttaagtg caactgatag cttatggcga atgccgcaat aaacttattt gttcatgacg
      181 tacattgtta cttcaaagcc gaaacgcatt tcggtagcag ctggtttagt ccacataatg
      241 atctcctttt taatttcccg aaccgacacc cgtctggtcc gtgtgaatgt attatcggct
      301 tgtgaactca aaatcctgct attagggatc atgaacaagg tttagggaat ttcatgaaac
      361 agccctcatg gcgtaagcat cagaaaaggt tttgcctacc ccagtgcacg acacatgtgc
      421 actcaagtca cctcttattc agtatcaatt aagccatgtt tcatggccag tctcaccatc
      481 tctaccgggc tattcacaga cagcttttgt ttaatcagcg tgtaataatt ggcaactgtt
      541 ttctggctga ttttcagcat atcggccaca tcttctaccc gcttgccttc tgccagtaaa
      601 cggaacactt caaactcacg tgaggtgagt tgttgcagcg ggttactttc gcctatcagc
      661 gtttgcaagg ccactttttg cgccacgtcc tggctcagga aagtgcggcc tttcgaaaca
      721 tccagtaccg cttgcaccag gtcatcagcc acgcctgtct tggtgacata gcccttgacg
      781 ccggttttaa gtgcctgggc cgcaaaagaa actgcctcat gcatggaaaa gatgacaatt
      841 tttgcctgcg gatagcgctg caaaatacgc ttggcggctt ccagcccgcc catgcctggc
      901 atggagatat ccatcagaat cacatcgggt aagacttcgc catacacttg atacgccatt
      961 tcaccagtat ccgcttcggc cacgatttct attgatttgt ggctttccag caaacggcgc
     1021 aaaccagagc gcacaacggt gtgatcatcg actaaaacaa ccttgactat catggcgcag
     1081 tatcctgaaa ttttcctata ttctgaaagt gtacttctat gcgtgtccct tgaccgggtg
     1141 cactcgtaat ttgcatggtg cccatcagcc cttctacccg ttcctgcatg cccgccaggc
     1201 cataaccacg gctgggcacg gctgggtcaa atccgcggcc atcatcttcg accagcaagt
     1261 acaatccgcc tgccttatcg ctcactgcta tggtcacccg gttagcggct gcatgcttgc
     1321 tgatattggt caagcactcc tgcaggatac ggtaaaccgt gacggcgacc gactcgtccg
     1381 gctgggtcaa atgattggaa atctcgttga cgaccatgat gctgcggttg cggtcatgcc
     1441 aatggtgaat cagctcggcc aatgccaggc tcaggcccaa ttcatccaat acgcgtggac
     1501 gcaaccgtgc cagcaaatcc tggatggtga acatcatttg ccgggtgatt tgtgcaatcg
     1561 ccttggcact ttcacgcgca acggtgagtt tctttgccgc aaccatggca ctggcgtcaa
     1621 tatgaacggc cgtcaggtac tggccgattt catcatgcag gtcacgtgcc agttttttgc
     1681 gctcttcttc ctgcaagtga atgatctgct gcgtcaggcg gtgattgttg cggatacttt
     1741 gctccagcgt actagccatc ttgttgaatt tatcgctgat ttcgctgagt tcaacctgct
     1801 caaaaccagg caggcggctc tgatactccc catgttcaat ctggtttaag ccatggataa
     1861 tctgcttaac cggtttaaac gtcgcccgca ccgtccaata cacaaacaac agcacactga
     1921 gcccgaacaa gccgacaatc gccagcaagc ctgaagtatc gtcccagatt tcacgcaact
     1981 cgaaactggg gtcaggcgta atgaccaact cgccgatcaa ccgcccattg aggatgatat
     2041 ttttgcgttt gggttcatta agcacactgg aaatgtcgag caacctgaca aaccagatcg
     2101 gtggatcttc acccgcatgc tcaacgcgcg tagagcggtt agactccctt aagcgaccac
     2161 tcacatcaaa aaaatccacc ttcaggtgtc gaatattggt caactcctcc agcctgaaaa
     2221 cagagacttc cccttccccg ctacggctta gccaaccaaa gtctgtcatg taatgcgcaa
     2281 tctgcgcatc cagcaggtgc atcaccagat tggtagtgga gtcgacttct gccaggacat
     2341 cttcccgcgc attctgaatg actgaaactg cccacgtcag taacaaccca agcagtaaca
     2401 gggtaataat cagattgagc ttgaactgta ggctgaacga tttcataaca ccgggctgat
     2461 tctaaagttt attctgcaaa aacgatcggg aatttttccc gacacatgca caaacaccca
     2521 ttataggagg actgtgcgca aatacgaacc cgcgtgccat accgcgaagc taagacgcgc
     2581 caaatacaat ggcgcgcctc accagattaa atattaccgg atcaccactc cccaaggcag
     2641 tgccccaact gggatatcct tgagtttagt attcgtttgc gtgtcgatca cagagacatt
     2701 attgctgcgc ccattggcga catagagttt tttgccgtcc ggcgttagtg ccatattcca
     2761 cgggcgctgg ccgacggaga tggtggcaat gatttgacgg ctggcggtgt caatgacact
     2821 gagattatga tcgccaccat tgctgacata aatacggctg ccatcggcat tgaccttaac
     2881 cccgttggag cgcaggccga ccttgatatt ggcttgtact ttccagtcgg cggtggagac
     2941 aatctctagc aggctggcgg cttcatttgc gacataagcc catttgccat cgggactaaa
     3001 accaacgccg cgcgggtgtt tggtggatgc gatcaggtgt tcggatttga gcgtgttgag
     3061 attaatcaca tccacgttgt tgctctcttc gttgctggcg agcagccatt gctggtcggg
     3121 tgaaaatacg cagtgctctg ggtttttgcc ctgggttttg atggtgtgtt tgagcttgtt
     3181 ggtttgcagg tctaccagcg agaccgcgtt ttcttcttcc aggcaggcgg ccagccactt
     3241 gccatcgggt gaaatatcaa taccttcggg ttcgtcacca actttgatca gggcggtttg
     3301 tttgccgagc tgggtgtcta ccaccgagac ggcgttttta tcgcgcacga cgacgtagag
     3361 tttttgcttg ctggggtgta tggcggcggc ctgcactttt ttgccgagct tgcctttttt
     3421 gggtagcgtg ttgatgactt ggtcggtgct ggtatcaatc accgtcacag tgccgtcttt
     3481 ttcattgggc acataagcga ggggttggga aaaaacgttt gaacagaagc tggccagcgc
     3541 gccggccagt atgaggtttg atagttttgt ttgcatagag cgtccttcaa aaaaatcggc
     3601 cagttgttgc aacaactggc ctgaacatgg agatatactt tatgcaaaat caattaaatt
     3661 caaaatttaa atgatgcttg catcataagt gttcaggatg atcatccata gcggcatgct
     3721 tcccgattta cccaggataa attcccgacg aaaaatcacg gtgcatgccc gataagctgt
     3781 ccaaatgcgc ggggttgcga ccctacctta atcgcggact tcaatacctg cgacgagtcg
     3841 tcaatcacgg ccagctcgtt atccatccaa ctggcaacca agatctgctg ccgcacggca
     3901 tcgtaagcaa tcccttccgg tgtgcctggc acgcgaatct tgcctgtggt tcgacggctc
     3961 gccagatcaa tcaccgtgac agagtcttcc ccggtattgg ttacaaacag gcgctgccca
     4021 cgatgatcgg ttgtcgcgca ataagggtga tcgcccaccg ccagggttgt caccactttg
     4081 tcatcatgcg tatccacgac gctcacggag tttgaataca cattcaccac aaacaattgc
     4141 cggccactcg gactcaggct tagcccgaaa ggatgctgac ccacagggat cctgcgcctc
     4201 tccttgtttt gtcgcgtatc caccacgctc acgtcattac tatcacgatt tgccacatac
     4261 aatgtcgcat catccaggct aagctgcatg cccgctggcg cgttacccac cttgacttgc
     4321 gcgatgatgc gcccgtcatc tgttgccacc accagcaggg tgtcgtggta ccagtccgcc
     4381 acataggcac gatcaccgcg gtggctcacc gtgatgccta ctggggcaat cggcaaggca
     4441 atggtgtttg ccagttgctg ccgttgcata tcaatcacgg agagcgaacg cccttcgaca
     4501 ttgctgacat aaaccttttg attaacctta tccacggcca cgcctaccgg ggctttccca
     4561 accttgatgg tattcaccac ttgctgcaat tgcaggtcaa ccaccgaaac agagtcctct
     4621 ccctgattgg tgatataggc atagtctgca gcctgcacac acaggcccag actcataccc
     4681 aatagagcgc acaacgtttt aatcatggag aacatccgtt tttgccaccg gagatggact
     4741 tgactgccat tgccaaactc gtgctgctac tataagcatt tgtctgacaa ttggagtgag
     4801 actccctccc gcaggtgtac aggaattctt cacctgcgat tcgggtaaag gctcccggca
     4861 tggcgctgcc ggttaagcga tggaagtgga ctggctaaag tgttcgttaa tcgccgtcat
     4921 gtcaaaggcc tgccacatct ggagatggtg ttgcaactcg tgggcatgca tggcaccggt
     4981 gacccgatca ataatcacgc gttcgcgcac caccatcaat gcgggtactt gcgccacctg
     5041 gaaattggcg gccaggtcgg tggccacatc aatatctgca atagcaaacg aaatggaagg
     5101 atactgagac gccacctcgt taaatgcagg ataaaaagcc tggcacgggt cgcaccactt
     5161 ggcggtaaac agtaacacca caaaatcgtg gccggccaca tgctggttaa actgctgctg
     5221 gtcatcaatc gcgaccggca gctgagggat agggttggaa tgcatgcatt actccttacg
     5281 atagggggca cctgccaccg gcaggcgctt gatggtagcc agggtccagt gttgcggtcc
     5341 ggcgatacta tgcgaacagc ccagctcagt ttccggcttg tccgaagtca gtacgtgcct
     5401 ggtgtggtct gcggtttgca cctgcataaa ccagcggtct acccatactt ccaccggacc
     5461 ttcttgcacc ggcaaggccg cctcggtcag ctgtaatgac accatttttc caccatgcgc
     5521 caggcagagt gaatcatccg tctcaaccag tcgaaccaat tcaagcacag caccttccgc
     5581 ctggcacaaa gggtgaacac ccaaacacac aaggaataaa aagggcagac ttacacgcat
     5641 gcgaaccctt taagcacaga ctgagatacc caggcttttg aacactggcc gcatcgccac
     5701 acacaccttg tggcgccagc cagacgaatt ggccgccgca ttacggccat ctttcaggcc
     5761 ctgcaaaccc tctttcacaa atgcgtctac ggcacttttc gctgactcgt catcttggcc
     5821 agcaggcggt tcatttccgg taaacgcacg gtaataacgt gccgtccaga ccactttggc
     5881 attgctgcca tcggcactta ccttgatttt gccgttgagt gaactgacag gcagcgcctc
     5941 aagatcaata tcgccagaca agcggtaaga caccgtcatg tcggcagcag caacctcatc
     6001 aatctcttgc agcaagtgct tgccattttt gagggtgagc ttttgctgtt tatcgcccac
     6061 tgcctcacag gcagtcactt gtggatgcca ttgtgcaaga gcacagggtg cactgatacg
     6121 cttccagacc gactctgccg atgcaccgac ctggatggac tcgtcagttt tctgcggtgt
     6181 cgggccatgc gcccacacat tcgcactcaa caacaatcca gccagtaata aagaccactt
     6241 catgtttact ccttctcgat aaaatctata gggatggttt gccagagggc ttgatcagcc
     6301 gcacccttgc gccgcgccaa gcgcgaccga acatgcccag ccagccacgc ccggcaaacg
     6361 tcagggccag gtaaaccatc caggccacac ccaccggcca ggccaaccag ggcctgagat
     6421 cagtatcggc ccagcgccag gcggtcattc ccagcccggt cgcccagctg cttccttgtg
     6481 ccacatcatc caggcggaca acgtgcaagc ctgtcgcatc gcccaggcgg cgcaggttct
     6541 gcgcgtccag cgccgacaag tgcgcatttt ccagcaaggt gttgcctggc ccatgtccgg
     6601 cattccggtc atgctggcct tgctccatgg ctaacaccga cagcgtttct gccatgccaa
     6661 aattcccaaa gcgctgcacc tcctcttgct cccaataacc ggagaccgca ttcttttcat
     6721 ccagcttggg gattttgctg ggcgtaggct tacccatccc gaccagatac ccctggacct
     6781 caccaggctt gcctgcgtag accggcatat atttcggatt tgcgggtggc gcttgctggc
     6841 cgtcggtaaa aaacatcagg cgcatgtgtt tgcctaaatt tggcgtttgc tcaagtgcgt
     6901 tgtacacacc atgggcaatg aaggagtcgg cggcccaagc catgcgccag tccatgctgg
     6961 ccactgtctg gtcgagcgca gcgaaatggc tgcagacttc gaccggtttg acgacagtaa
     7021 ccacattgcg ctcggtaaac atgcccaggg ctacttgcga gccacaaggc aaggctgaca
     7081 gcgttttccg tatgctctgc ttagccattt gcaagcggct cacgctttta ccggacagct
     7141 ggtaatcaga cacattcatg ctctgagtaa tatccaccac aaaataccag tcgtacacgc
     7201 ggttgggcag cacccaatgt ggtttgaaca aaatcaatgt gagcaatacc agcatcagga
     7261 acaggcaacg ctggcgccaa tccgcctgtc gtttccctgc catcaaccag cgaggcatcc
     7321 agcgtctgag acgcgcagcc agtggcccca aaaaacgctt cgccatcagg gcattcctcg
     7381 tggattgcca ggcatggctg gccagccgct gggctcttgc tcggccttga tttgctcatc
     7441 ttcgtattgc tgtggcccgg acatcccatg cgtggtgggt gccagccgta gtgccaggtg
     7501 gtagttatat ttcacttccg accattcagg cgctatgcgt aaagccttct ggtaactttc
     7561 cttggccagc gccaccagcg gcccggcttc gtcccaggct ggcaggcccc gctgctcaag
     7621 caaacgcgta gccgtccgca tatacacatt gccagagttg aaataggcct gtttacgcaa
     7681 gggatcatcc tcccctaccc ggctcgccac cgcagcatat tgggtcagcg cctgctcagg
     7741 cttgagctgg ttgtcataaa acacggcatt ggcaaacagc gcctgcgggg tctggtttgc
     7801 ccccagcatg ggttgggtaa tcagtacgtg cggttccagg ctgctacgat aaatctgggc
     7861 ctggtaaagt tcgatcaact gcctggccag catgaccagt gaaatcacga tgatcacgct
     7921 tgcccaaagc agcccacgcc gcatgacacc tgccagtttt gcattcatga ctgccactgt
     7981 ttcaactcca atccaaacat tgctaccagc aacacggctg ccagccatgc cagaaagtag
     8041 gcatagtggc tcaagtcctg ccgtgcgact ggttcgaggt aacgggtagg tttattttta
     8101 agggttgaga tatcgcgcac ggcagccatc actgcctccg gattttcagc ctcataaata
     8161 cggtaaggca cattcaaggt ctggaaatac tcgtgcaggg catactcggg ataagcatca
     8221 atattttcac cttctgccag gctctgtttc aggctgacgc cactttccga acgcaggtaa
     8281 atccagtaca gcgaggcctg ctggcgcatg aacatagcgc gcaacatgtc ctgggtttta
     8341 ccatccaggt gtgccccgcc atccgagacc aacaccatgg cgcgtgcgcc cgtgactggc
     8401 tggtcggcaa agtaatcaag cgccataccc aggccacgcg ccacggctgt gaatcccatg
     8461 ccgccggcct cagtggcatc cagtgcagcc tgcacaaagg cacggtcatt acccagcggc
     8521 gcagcaagaa taggtgaggt actgaaggtc accatgccca ttaagtcgtc atgcccctgg
     8581 gcgaccagcg tcttcaatac attgcgcgcc acctccatct tggggaccct cgcctgggca
     8641 tctccctttt tgacaaacgg ctcattcatg ctggcactgc gatccagcac aatcatgaca
     8701 tgtgcaccac gccccacttt ttctatcatt tgtgacttca ggtaaggtcc ggccaatccc
     8761 aggctcagcg agagcatcag caggctcagt gccatcttcc atagccgatc caaccaggca
     8821 gacaaagggt cttccggcaa cccttgcaac gccggatagg caaaactctg ctgccctcgc
     8881 aacagccatg gcaacatcgc cagtggcagt agcagcaagg gccagagctg cgtcagtccc
     8941 cagtgttcgc cataccccgt cgccagcaat aattgctgcg tgccgtcatg ccacatactc
     9001 aggaaatctc cacctggctc agttgtttgg ccagcttttt caactcacgc aacgacaaac
     9061 ttgcttgttg cccggcaaaa aaactgtgct gcaccgcttg ataaaactga cgcagcgatt
     9121 cgctgtaggt atccagcgca ggatgcatgg tcagcagtct tggcaggttt tcggtagtga
     9181 tggcataccc ggcaaattgg tccatggcgc ggctaatcag gcacatggct tgcaaggaac
     9241 ttgcatggcc ttctggtaac gcccggattg cccgccaggc ttgcctgaag ggcatgcgtt
     9301 tttcctgggg taactgaatc cagcccatgt accaacacag gtacagcgtc gccagcccca
     9361 ataagcacaa ccagccagcc agtttccaca actggttgga cagggaccac tctggcaatg
     9421 gggcatccgg atacaacttg acatgctcgg catcaatacg cgcaggcagc aggctggaaa
     9481 caatcacctt gtcagcgggc acatccacca ttagccgctg ctggtcacgc ttgaattcca
     9541 gatgcaggtt tttcaatgga atggttttca cggtatcgcc cgccgcaaaa atctgccagt
     9601 caatttccag ctgaatcagg cgctcgttgc ctgaatcagt ggtcgtccag tgtgcgtccc
     9661 gcaattcaat cgcttcgttt tgggcaggta gtggcaggct gccggggtcc agttcgaatc
     9721 ccaaaggaac atgcagggtc aggtgttgcc gggcgatgct gcccagcgtg taatctgttt
     9781 cgcgggtcgc actttcggca cgaacttgca tgccctccgt ggtgaccgca gcacatgcct
     9841 gacctgagcc taactgcagt aaaaccaggg cagccaacca cacccggcat gttgtattct
     9901 gatgggtatg caatccggca ttcatgccat ggtctccatt aagtgacggc tgatgtcttc
     9961 agcccgccag cccggacgca accacaaggg tgcacgcatg ccagccttta aaaattgggc
    10021 atccaatgcc tgtgcctgct gctggccata atcggcaatt tgttgatgca gccggcgcct
    10081 gagcatccag ctgcgctctt cgccagtttc catatcccgc aaacgtgccc agccaaaggc
    10141 aggtagtccc gtggtcgctg cacggtcccg caacaccacg gggaccacat catgcgattg
    10201 cgcggccttg agtacttgtt gcatcagggc atcaggccag tagaagtctg acagcaggaa
    10261 taccagcgcc cgccgtgccc ccagcgattc aatcgcagcc ggaatgccag cggccccatg
    10321 tcctttttcg ccagctgctg tatcttgcca gtaccgcgtg attttctgtg ccacgccctg
    10381 cgccgttgca ggctgaaacg tcggcatgac ctgccagacc gcttggggcg tgtcatgcgc
    10441 cccccgcata ccaaaggcat cgccctgacg ggtagcagac caggccaatg aagacacaaa
    10501 ttctgtcacc tgctggcgca gctgatcatg cccttgaaac cgcatggagc ttgagagatc
    10561 taccacggca tacacatgaa tggccgcgcg ttcgagataa gtcctgacca accattgccg
    10621 gggcgtgcta cgcacactgg cacgaatatc aatgcgcttg ggatccgggc aggataacaa
    10681 cggggcaaaa ccggcaaaat cgctgccatt gccatgatgg ccggtcaggg tagcacccgc
    10741 gtggtaggac cttgatcgcc agttgatatt gtaaaaaaac aaccggggtt caaacgtcgc
    10801 gtgcacttcc ggcgcagggc tggagaggtc tgcagacact gaggaaagtc ccatgaaaaa
    10861 cggcctcacc tattcgttta aggtgcagaa actttatcta gcaccgcttg cagcaacatc
    10921 ggtgccaaac gttcgtgctg cagcgcatag gcgccgttga agaatacccg gtgcgtcacc
    10981 acaaatggcc acacggcacg gatatcgtcc ggcgtcagat aatcccggcc ttccagccag
    11041 gcgcgcgtac gcccgctacg catcagcatc gccatgccgc gtggactggc gccacctgcc
    11101 aacaatcttg acatatcgac atccggcaat tgaatcccgg ctgcctgggg atctttgagc
    11161 gtgcgccaca aatccacggc gtaatcctgt aacggcactt ctgcccgcac agaggcctgc
    11221 accgcttctg ccacttggtt taactccgca taaggcgtta atccaaccgg caacccatca
    11281 accaaacggt caacatcata aaaacgcgta tcaaacatca gggcgcgttg caggtcacgg
    11341 gcatccgggg ccttgatcga aatttccatc aagaaacggt cgcgagccgc ggccgggatt
    11401 tcaaaggtct cttcacgttc taaacggttt ctatctgcaa acacggtcaa atgcgggaaa
    11461 tggtattcac gattaaaggc attgaccgaa cgctcggcca tcaggcgcaa gaacaaggca
    11521 tgtgcctgtg ggcgggcacg gttgatttcg ttaaaaaaga aaacggataa agcctcatca
    11581 tgctgcaata acggccccgg cttgactgcg gcctgaccct gctcatcaat ataggcatga
    11641 tagagaaagt cactgggcat caggtcaatc gccccttcaa tacgttggta accgccacca
    11701 agcaaatgcg ccgcagcgcg tagcagggta gttttaccaa cacccacatc accttccagc
    11761 aatacatgcc cacgcgaaaa aatcgcaatg gccagggcgc ggatttgctc cggcatcccc
    11821 accacaacac tgttcaatgc ttgctcaaac gccagcgcac gttcacgcca tgctggcaga
    11881 tccactttat tttccatact tattccatga tccgggatga aacaggccac tgtcgactgc
    11941 ctgcaatcga caccggtttg ccacatacac ggggatgtga aaac
//
LOCUS       DATMSK010000027        12470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_213316,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000027 DATMSK010000000
VERSION     DATMSK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213316"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..3933
                     /gene="purL"
                     /locus_tag="VNZ84_00885"
                     /gene_synonym="purI"
     CDS             22..3933
                     /gene="purL"
                     /locus_tag="VNZ84_00885"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HWU65533.1"
                     /translation="MSSQAHFFSLRGSAALSQFRLDKLYSALNTSAPNIAHIYTEFVH
                     FAFSETPLSTTEQDTLRQILTYGPKTDIESPSGELLLVIPRIGTISPWASRATDIAHN
                     CGLGSLLRLERGIAYYMTSKNGQPLTDAEKQALRAAIQDRMTETVVYSLADAEKLYHH
                     ADPKPLSCIDILAGGKVALEAANNDMGLALSPDEVDYLLENYRKMGRNPTDVELMMFA
                     QANSEHCRHKIFNADWVIDGVQQELSLFNMIRNTHKLNPGHTVVAYSDNSSIVAGQKT
                     KRFYPSADQSYQFVEDDMHYLMKVETHNHPTAISPFAGAATGAGGEIRDEGATGIGSK
                     PKAGLAGFSVSNLNLPGFEQPWETNYGKPGRIASPLQIMIDGPLGGAAYNNEFGRPNI
                     AGYFRTFELETAGADGKAEVRGYHKPIMLAGGVGNISDSHSHKNSIPAGAALIQLGGP
                     AMLIGLGGGAASSMDTGANTENLDFDSVQRGNPELQRRAQEVIDRCWQMGDKNPILSI
                     HDVGAGGISNAFPELVNDAKVGAVFQLRDVHNEEPGMSPRELWSNEAQERYVLAVTQD
                     QLPLFEALCERERCPFAVVGVTTEERHLTVQDRHFANNPVDMDLSVLLGKPPKMLRDV
                     KSTKKSLTAFDTSKVDLHDAVARVLRLPGVADKTFLITIGDRTVTGLIARDQMVGPWQ
                     VPVSNVAVTCAGFETNIGEAFAIGEKAPLALIDAPASGRMAIGEAITNIAAASISDIS
                     NLKLSANWMAPAGHAGEDAALYATVKTVGMELCPALGISIPVGKDSMSMKTVWQDNGE
                     NKAVTAPISLVISAFANTPDVRKTMTPQLRTDLGDSELILIDLGNGRNRMGGSALAQV
                     YKQLGNAVPDVDQPAQLKAFFTAIQQLNTDGKLLAYHDRSDGGLFVTLAEMAFAGHCG
                     LNVDLSSFGNDAISTLFNEELGAVVQVRAADAAAVTAQLQQTLGACVHRIGQVNAGHD
                     IVIKQGNTQYQASRIDLHRMWSETTYRMQSLRDNPTCAQQEYDRILNADDAGLHAQLT
                     FDLNDNIAAPYINTGKRPNMAILREQGVNGQTEMAAAFDRAGFNSVDVHMSDVISGRV
                     SLKDFAGLVACGGFSYGDVLGAGEGWAKSILFNSRARDEFSAFFDRADTFALGVCNGC
                     QMMSNLHSIIPGADHWPHFVRNKSEQFEARVALVEILPSPSIFFNGMVGSRMPIAVAH
                     GEGFTEFTDASAVTSVLQQQLATVRYVDHAAQATETYPFNPNGSPQGLTGFTTQDGRF
                     SIMMPHPERVFRTVQHSWHPDGWQEDGPWMRMFRNARKFAG"
     gene            4014..4499
                     /locus_tag="VNZ84_00890"
     CDS             4014..4499
                     /locus_tag="VNZ84_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWU65534.1"
                     /translation="MKSVKFLVALFSLSFALQAFALSDEEYMEFTEALTGGNVKVVKK
                     FVEADPTLVKQKFFAWEPLQMAASKGRLDVVKYLVSKGADLNYVHPASHNTAFHMSAF
                     IGDTATMKYLASAGADVNIKLKGDVSLVRYFKEEGNEKMVDLLTELGTKNDGCQEEKC
                     F"
     gene            4659..5015
                     /locus_tag="VNZ84_00895"
     CDS             4659..5015
                     /locus_tag="VNZ84_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWU65535.1"
                     /translation="MQRLTVTLLWFFFTFATSVAQAEDPSTVTTRTLAASCAACHGPD
                     GNSLGGTPTLAALNQQYFVQRMQGFKDGSVSATVMHHHAKGLTTEEITALAAYFAQQP
                     RQTPHSLPHQSFLGAQ"
     gene            5015..6298
                     /locus_tag="VNZ84_00900"
     CDS             5015..6298
                     /locus_tag="VNZ84_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU65536.1"
                     /translation="MKPDTTALTPSRRQFVRSVIYGGLAFYGLPAFARASKPPLGHVV
                     VVGAGFAGLTAAKYLREWSLGNLKVTIIEPNPQFISCPQSNLVLGGSRSLDQLSFSYD
                     ITRKQHDLQWIRDSVIAIDMLNKQVKLTRGSVSYDRLVLAPGVDFNYGKIPGMPAPVD
                     AIPHAWKAGKQTLQLRQQLEDMPDGGLFVMTIPSGPYRCPPGPYERACQVAHYFKQHK
                     PRSKVMILDANADIVSKKGLFLQSFNGAYQGMIEYHNNSEILQLDTSSKTIKTDFETV
                     KADVLNVIPPQLAGKVAQAAGLNNVDNRWCEVDFLTYASSLQPDVHIIGDSISAGLPK
                     SAHMATSHAKVCAAAIIDLQSGNVPNPMPVFANTCYSFVDDQQAMHVANVYRYDPAKK
                     SMVSAEGGGVSAKASVQEGEYAQAWAQNIWADTLT"
     gene            6295..7509
                     /locus_tag="VNZ84_00905"
     CDS             6295..7509
                     /locus_tag="VNZ84_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWU65537.1"
                     /translation="MTTAQAQTSPANKVSRFLLMVLAGLCMAIGLHYAVAQTPAVDAA
                     LSTLGVPAGLKIEQFSDVSAWGAPRMLALDTQGRLLVSLSDTGRILRLNQQGEAEVIT
                     QGLNAPHGLALLGDDLLVAEQTGVVKLPKQGDGWGAPQPLIRNLPSGGHSLKTVKVSP
                     DGYIFINVGSSCNVCLEDNPLRATLLRYTADGRPAGAVLTVGRHAQSAIWSTGLRNTQ
                     GFTWHPGTGDMFATNNGADNRSAIKNGKTDDDLPPEHLNKIEAGKHYGWPYCWGDPGQ
                     PGQLFQDPNFTGEPDICKQAQAPALTMPAHSTPIGITFLQHSRLPTQIQQDAIIALHG
                     SWNRKQPSGYALLRVQFRNQLPVAAVPFISGWLQGQKAWGRPVDVIVGGDGWLYVSDD
                     LTGWIYRIRSTQ"
     gene            7550..8134
                     /locus_tag="VNZ84_00910"
     CDS             7550..8134
                     /locus_tag="VNZ84_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65538.1"
                     /translation="MKRILLSLSSALCLGWIGLSQAGAQPSLVIQVPEAFYQHPVRLL
                     NPYLNYWHNRAEAAETVGLSSFQSKNYTTSSCEAEAKGQALVVIEPNMFYNPQTGIFY
                     SEITAKVYNQPAADSALGKPLMMVKGQGQSRGWLTYNVEYFTHQSYQQAFDQVIQQLQ
                     QSPAFQQSVEQAPVQAYQALCNSIDTLAQPKAFF"
     gene            8143..9027
                     /locus_tag="VNZ84_00915"
     CDS             8143..9027
                     /locus_tag="VNZ84_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWU65539.1"
                     /translation="MLSNRHHRRTLFSSILASFLMITGSTGYSHGLEPVSGLLTPESA
                     VQAADGKIYVSEINGFGQDGDGQIRVIDHGKSSVLVQGLDDPKGLVIIGNDLYIADKS
                     RVLRIALNQETASAEVFLAATDFPKVPQFLNDLSADANGNLYISDSGDILGTGKGGAI
                     YKVTPQRKLSLLLDGNIDARILAPNGLLADAKGEHLLLVDFTSGVLYNYNQVTKKLEE
                     LANGFGGGDGIVKQANGNIYVSDWKNGKVFRVDPQHKVSLIKEGYQSAADIALTQDDH
                     HLLVPDMKAGKLEIIDLR"
     gene            complement(9158..9343)
                     /locus_tag="VNZ84_00920"
     CDS             complement(9158..9343)
                     /locus_tag="VNZ84_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65540.1"
                     /translation="MKKTMLLILGIIFSVCVWAGTNPSQHDQVEMQKHLKVAKHAGAQ
                     PVVTPQKEADKKTAQTK"
     gene            9538..10215
                     /locus_tag="VNZ84_00925"
     CDS             9538..10215
                     /locus_tag="VNZ84_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU65541.1"
                     /translation="MRILIVEDDLVIANGLVRALAQSQYAVDHVTDGKQALRAIQDDV
                     YDLVILDLGLPTMDGLQLLSHLRQQANPIAVLILTAREDVATRVEALDLGADDFLTKP
                     FHLEELEARVRALIRRSKLGNNLPLQCGNLKLDVANKLASINEQPITLSARELNLLET
                     LMMRMGKIIAKEQIMESLCNWDETLGDNAIEVYVHRLRKKIEPSGAHIRTVRGLGYML
                     ESEHLNP"
     gene            10225..11667
                     /locus_tag="VNZ84_00930"
     CDS             10225..11667
                     /locus_tag="VNZ84_00930"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWU65542.1"
                     /translation="MMGPRSLKNQLRLWLLLTMLLVLGVSAALSYSRALHYATQAYDQ
                     ALLRTVLALADEVIIDANNEVKIEIPEVASHLLSYNEGDRIYIRISAPNGKLVFGEAN
                     LPPPAQAPAGNQQVYYNSHYKNEAIRAVTFGLPESNASNALNILITMAETTGKRDAMV
                     AEMVEEMLLPQVLMMILAAIVIELGIHFALKPMQDLRESLHQRSHRDLSPLDTSASPL
                     ELQPLLQAMNALLSRVKAGIQQQQQFIADASHQLRTPIAGLQTQAELALRSHPPAEVQ
                     EPLNYMLRSTTRLSHLIQRLLSLARTDAASSQLVFKPVSLTSIIYDECARWVGPASDK
                     QIELEVLIETQQDQVLGDALMLSEMLNNLLENAIQYTPEQGLLRIRLWQAGKHLLLQV
                     ADSGVGIPADQTALIFERFHRLDPNQGNGCGLGLAIVAEIAEHHNASISVAQGLQHPI
                     THHLGSQFTVKFERYDNLPENPRATSTDAR"
     gene            11618..12427
                     /locus_tag="VNZ84_00935"
     CDS             11618..12427
                     /locus_tag="VNZ84_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWU65543.1"
                     /translation="MTTCLRTLALQALTLDDPQQKCTQVQLLWDATLAGTLYVNAPLS
                     LQTPEHAIPGRPAKPLLVPPKHVQRRAMHTAEGRAALIHALAHIEFNAINLALDAVWR
                     FTGMPEDYYRDWLQVAKEEAYHFSLLREHLQSMGFDYGDFDAHNSLWEMVEKTQSDVL
                     ARMALVPRTLEARGLDATPPLKQKLMQAGDHAAAAILDIILRDEIGHVTIGNRWFNFL
                     CDQRHINPMHTFEQLCLQFKAPKLKPPFNLAARKQAGFSELELAYLDGLTA"
BASE COUNT         3155 a         3278 c         3162 g         2875 t
ORIGIN      
        1 attgaaaaat ccaaccgtca catgtccagt caagcgcatt tctttagcct gcgcggcagc
       61 gccgccctct cgcaattccg tctcgataaa ctgtacagcg cacttaacac cagcgctccg
      121 aatatcgccc atatttatac cgaatttgtg catttcgcat ttagcgaaac cccgctgagc
      181 acgaccgagc aagatacgct acgccaaatc ctgacttacg ggccgaaaac tgacattgaa
      241 tccccaagcg gcgaactgct gctggtgatt cctcgcatag gcaccatttc gccctgggcc
      301 tcacgtgcga ctgacattgc tcacaactgt gggttgggaa gcctcttgcg cctggagcgc
      361 ggcattgcct actatatgac cagcaaaaac ggtcagccac tgaccgatgc tgaaaaacag
      421 gcattgcgcg cagccattca ggaccgcatg acggaaaccg tggtgtacag cctagcggac
      481 gccgaaaaac tgtatcacca tgccgacccc aaaccactgt cctgtattga tattctggct
      541 ggtggcaaag tggcgctgga agctgccaac aacgacatgg gcctggcttt gtcgccggac
      601 gaagtcgatt acctgctgga aaactaccgc aagatgggcc gcaacccgac ggatgtggaa
      661 cttatgatgt ttgcgcaagc caactctgag cattgccgtc acaaaatttt caacgcggac
      721 tgggtaattg atggcgtaca gcaagagctg tcactgttca acatgatccg caatacgcac
      781 aagctgaacc caggccatac tgtcgtcgca tactcagaca actcgtctat cgtagcaggc
      841 cagaaaacca agcgtttcta tccctctgca gaccaaagct atcagttcgt agaagacgac
      901 atgcactacc tgatgaaggt agaaacgcat aaccacccga cggcgatttc accatttgcc
      961 ggcgctgcca caggtgcagg tggcgagatc cgcgatgaag gtgcgaccgg cattggctcc
     1021 aaacccaaag caggcttggc tggtttttcg gtctcgaacc tgaacctgcc aggtttcgag
     1081 caaccctggg aaaccaatta cggcaaacct ggccgtatcg ccagcccact gcaaatcatg
     1141 atagacggcc cattgggcgg cgcggcctac aacaacgaat ttggccgccc taatattgcc
     1201 ggttacttcc gtacgtttga actggaaacg gctggcgctg atggcaaagc tgaagtgcgc
     1261 ggttaccaca agccgattat gctggcgggt ggtgtcggta acatttccga tagccatagc
     1321 cataaaaaca gtattcctgc cggtgctgcc ctgattcaac tgggcggccc tgccatgttg
     1381 attggcctgg gcggtggcgc ggcctccagc atggatactg gcgccaacac cgagaacctg
     1441 gactttgact ctgtgcagcg aggcaaccct gaactgcaac gtcgcgcgca agaggtgatt
     1501 gaccgttgct ggcagatggg tgataagaac ccaatcctga gtattcacga tgtgggtgct
     1561 ggtggcatct ccaacgcttt ccctgaactg gtgaacgacg ccaaagtcgg cgcggtattc
     1621 cagttgcgcg atgtgcataa tgaagagcct ggcatgagcc cacgcgaatt gtggagcaac
     1681 gaggcacaag agcgttatgt attggcagtg actcaagacc agctgccgtt gttcgaagcc
     1741 ctctgcgagc gtgaacgctg cccgtttgcg gtcgtcggcg tgaccactga agagcgccat
     1801 ttaactgtgc aagaccgcca cttcgctaac aatccagtcg atatggattt gtccgtgtta
     1861 ttgggtaaac caccaaaaat gctgcgcgat gtgaaatcaa ccaagaaatc actcacagca
     1921 tttgatacca gcaaggttga tttacacgat gccgtggcgc gtgtattgcg tttgcctggt
     1981 gtggctgaca aaaccttcct gattaccatc ggcgatcgta ccgtgaccgg cctgattgca
     2041 cgcgaccaga tggtaggccc atggcaagtg ccggtcagca acgtggccgt gacctgtgcc
     2101 ggttttgaaa ccaacatcgg cgaagcgttt gccatcggtg aaaaagcacc actggcattg
     2161 attgatgcac cggcttctgg ccgcatggcg attggcgaag ccatcaccaa cattgctgcc
     2221 gcctcgatca gcgacatcag caacctcaaa ttatctgcca actggatggc ccctgctggc
     2281 catgctggtg aagatgccgc gctatatgcc accgtcaaaa ccgtcggtat ggagttatgt
     2341 ccggccctgg gtatcagtat tccggtgggc aaagactcca tgagcatgaa gaccgtttgg
     2401 caagacaacg gcgaaaacaa agctgtgact gcgccgatct cactggtcat ttctgcgttt
     2461 gccaacacgc cagatgtgcg caaaacaatg actccgcaac tgcgcactga tttgggcgat
     2521 agtgagctga tcctgatcga cctcggcaat ggccgtaacc gcatgggcgg ctcagcattg
     2581 gcgcaagttt acaaacaact gggcaatgcc gtccctgacg ttgaccagcc agcacaattg
     2641 aaagcgtttt tcacggcaat ccagcaactg aatacagacg gcaaactgct ggcttaccat
     2701 gaccgctctg acggtggcct gtttgtgaca ctggctgaaa tggctttcgc gggtcattgt
     2761 ggccttaacg tcgatttgtc cagttttggg aatgacgcga tcagcacctt gtttaacgaa
     2821 gaactgggcg ctgtggtaca agtacgtgcc gcagatgccg cagcggttac cgcacaactg
     2881 caacagacgc tgggcgcctg cgtgcaccgc attggccagg tcaacgccgg ccatgacatt
     2941 gtcatcaaac agggcaatac gcaataccag gcctcacgca ttgatttgca ccgcatgtgg
     3001 tctgaaacca cttaccgcat gcaaagcctg cgtgataacc cgacctgtgc ccagcaagag
     3061 tacgaccgca ttcttaatgc cgacgatgcc ggcctgcatg cccaactgac gtttgacctg
     3121 aacgacaata tcgctgcgcc ttatatcaac actggcaagc gcccgaacat ggctatcttg
     3181 cgtgagcaag gcgtgaacgg ccagaccgaa atggccgctg cgtttgaccg tgcaggcttt
     3241 aacagcgtgg acgtgcatat gagcgacgtc atcagtggcc gtgtgtcatt gaaggatttt
     3301 gctggcctgg tggcttgtgg cggcttctct tacggtgatg tgctcggtgc cggtgaaggt
     3361 tgggcgaagt ccatcctgtt taacagccgt gcgcgtgatg aattcagcgc attctttgac
     3421 cgtgcggata cctttgcgct aggcgtttgt aacggctgcc agatgatgag caacctgcac
     3481 agcattatcc caggcgctga ccactggcca catttcgtgc gcaacaagtc cgaacagttc
     3541 gaagctcgcg tggccctggt cgagatttta ccttctcctt ccatcttctt taacggcatg
     3601 gtaggcagcc gtatgccaat tgcggttgca catggtgaag gttttacgga atttactgat
     3661 gcgagcgcgg tcacaagtgt gctacaacag caactggcaa ctgtgcgtta tgtggatcat
     3721 gctgcacaag cgaccgaaac atacccgttt aacccgaatg gttcgccaca aggattgacc
     3781 ggatttacca cgcaagacgg ccgcttcagt atcatgatgc cgcatccgga acgggtattc
     3841 aggactgtgc agcattcctg gcatcctgat ggatggcagg aagatggtcc atggatgcgt
     3901 atgttccgta acgcacgcaa gtttgcgggt taagcgaaat cattgcgttt atgctttcgc
     3961 cattaaaaca aagttgttat ttttaaaaat gaaatcaggt gtttggagag attatgaaat
     4021 cagttaagtt tttagtggcc ttgttctcac tgtcatttgc gttacaggca tttgccttgt
     4081 ccgatgagga gtacatggaa tttaccgaag ccctgacagg tggtaatgtt aaagtggtca
     4141 aaaagtttgt tgaagccgac ccaacactag tgaagcagaa attctttgcc tgggagccat
     4201 tacagatggc cgcctccaaa ggcagactgg acgtggtgaa gtatctggta tctaaaggtg
     4261 cagatctgaa ctacgtacac ccagccagcc ataatacagc gttccatatg tcggccttta
     4321 tcggggacac tgccaccatg aaatacctgg cttctgcagg tgctgatgtc aacataaaac
     4381 ttaaaggcga tgtttcactg gtacgttact tcaaagaaga aggcaacgag aaaatggtgg
     4441 acttgctgac agagcttggc acaaaaaatg atggttgtca ggaagagaaa tgcttttaat
     4501 cgtctgtgag tagcaacgat atcaatgcaa aacggtcatg agattcatgg ccgtttttgt
     4561 tttaagtgcc agtttggctg gcaagtgtaa ccatagtcac gcacacgtac tgcctattga
     4621 gaataatgtg acaccaccaa caataaaccg ggggaagtat gcaacgttta actgtcacgc
     4681 ttttatggtt tttcttcact ttcgccacat ctgtggctca agcggaggat ccgtccacag
     4741 tcaccacccg cacgctggca gcctcatgcg ctgcttgcca tggcccggat ggaaacagcc
     4801 tgggtggaac gccaacgcta gctgcgctta accagcaata ttttgtccag cgcatgcagg
     4861 ggtttaaaga tggttctgtc agcgctaccg tgatgcacca ccatgccaag ggcttaacta
     4921 cagaggaaat cacggcgttg gcggcttatt tcgcacaaca accaaggcaa acaccgcatt
     4981 ccttaccgca ccaatccttt ctgggagcgc aataatgaaa ccggatacta ctgctttaac
     5041 gccctcccgc agacaattcg tacgctcagt catttatggt ggtctcgctt tttatggctt
     5101 gcccgctttt gcgcgtgcca gtaagccgcc attggggcat gtcgtggtgg tcggtgctgg
     5161 ctttgctggc ctgactgcag ccaaatacct gcgcgagtgg agtttgggca acctgaaggt
     5221 cacgatcatt gaaccgaatc cgcagttcat ttcctgcccg caaagcaacc tagtgctagg
     5281 tggtagtcgt agcctagacc agctaagctt cagctatgac atcacacgta aacagcatga
     5341 cttgcaatgg atacgagata gcgtgatcgc aatcgatatg ctgaataagc aggtcaaact
     5401 cacacgggga agcgtcagct atgaccgcct ggtactggcc cccggtgtag atttcaatta
     5461 tggcaagatt ccgggtatgc ctgcaccggt cgacgctatc ccacatgcct ggaaagctgg
     5521 taagcaaacc ttgcaattac ggcaacaact ggaagacatg cccgatggtg gcttgttcgt
     5581 catgaccatc cccagcgggc cttatcgttg cccgccagga ccttatgaac gcgcttgcca
     5641 agtggcacat tatttcaagc aacacaaacc gcgcagcaaa gtgatgatcc tggatgcgaa
     5701 cgcagatatt gtatcgaaaa aaggactatt cctgcaaagc tttaatggcg cttaccaggg
     5761 catgattgaa taccataaca acagcgagat tttacaactc gacaccagca gcaaaaccat
     5821 taaaactgac tttgaaacag tcaaggccga tgttctcaat gtcattccgc cacagctggc
     5881 aggcaaagta gcacaagcag cgggccttaa taatgttgat aaccgctggt gcgaggtgga
     5941 ctttttgacc tatgcttcca gcctgcaacc cgatgtgcat attatcgggg acagtatttc
     6001 ggccggcttg ccgaaatcgg cccatatggc gacctcacat gccaaagtat gtgctgcagc
     6061 cattatcgac ttgcagtcgg gaaatgtacc taatccaatg cccgtgtttg ccaatacttg
     6121 ctacagcttt gttgatgacc aacaggccat gcatgtggcc aatgtttacc gctatgatcc
     6181 ggccaaaaaa tccatggtgt cggctgaagg tggtggcgta tctgccaaag cctcggtgca
     6241 ggaaggcgaa tatgcacagg cctgggcaca aaacatctgg gcggatacat tgacatgacc
     6301 acagcgcaag ctcaaacgtc tcccgctaat aaagtctccc gtttcctgct aatggtgctg
     6361 gcaggactgt gtatggcgat tggattacat tacgctgtcg cacaaacgcc tgccgtggat
     6421 gctgcattat ccactctggg cgtgcctgcc ggactcaaga ttgagcagtt cagcgatgtc
     6481 agtgcctggg gtgcaccgcg gatgctggcg ctggatacgc agggacggct gcttgtcagc
     6541 ctcagcgata ccggccgcat tctacgcctc aatcaacaag gcgaagcaga ggtcattaca
     6601 caaggcctca atgcgccgca tgggctggca ttgctggggg acgatttgct ggtggctgag
     6661 caaactggtg tggtcaagct gccaaagcaa ggcgatggct ggggagcgcc acaaccactg
     6721 atccgcaact tacccagtgg tggacatagc cttaaaacag ttaaagtctc accagatggt
     6781 tatattttta tcaatgtggg ctccagttgt aacgtttgcc tggaggataa cccactccgc
     6841 gccaccctgt tacgttatac ggcagatggc cgtcctgccg gcgcggtgct caccgtcggc
     6901 cgccacgcgc aaagtgctat ctggagcacc ggattgcgta atacacaagg gtttacctgg
     6961 catccgggaa ccggagacat gtttgccacc aacaatggtg cagataaccg cagcgccatt
     7021 aaaaatggca aaacggatga tgacctgccg cctgaacatc tcaataaaat cgaagcggga
     7081 aaacactatg gctggcccta ttgctggggc gaccccggcc agccagggca attattccag
     7141 gaccccaact ttactggcga gcctgatatc tgcaaacagg cacaggcacc tgctttgacc
     7201 atgcctgcgc acagcacacc tatcggcatc acattcctgc agcacagccg cctgccaacg
     7261 caaatacaac aagatgcaat tatcgccctg cacggctcct ggaaccgcaa acaacccagc
     7321 ggctatgcct tgctgcgtgt acaattcagg aaccaactgc ctgttgcagc cgtcccattc
     7381 attagtggct ggctacaagg tcaaaaagcc tggggaaggc cagtggacgt gattgtcggt
     7441 ggcgatggct ggctctatgt ttcagatgac ctgaccggct ggatttaccg aatacgcagt
     7501 acgcaataaa agccttgaat ggctaacgaa tatttttcag gagcaaatga tgaaacgcat
     7561 tttattgtcg cttagcagtg cgctttgcct gggctggatt ggcctgagcc aggccggtgc
     7621 gcaaccttcc ctggtgatcc aagtcccgga agctttttat cagcatccgg tacgcctgtt
     7681 gaatccttat ttgaattact ggcacaatcg ggctgaagct gccgaaacag ttgggctcag
     7741 cagcttccag tcaaaaaact acaccacctc cagctgtgag gcagaagcca aaggccaggc
     7801 gctggtcgtg atagaaccaa acatgtttta caacccgcaa accggtatct tttacagcga
     7861 gattaccgcg aaggtctata accaacccgc ggctgacagc gcactgggca agccactaat
     7921 gatggtgaaa ggccaaggcc aatcacgcgg ctggctgacc tacaatgtag aatatttcac
     7981 tcatcagtct tatcagcagg cgtttgacca agtcattcag caattgcagc aaagcccggc
     8041 tttccagcag tccgttgagc aggctccagt acaagcctac caggcattat gcaatagcat
     8101 agacaccttg gcccaaccaa aagccttttt ctaggagcgt ttatgttgtc aaaccgtcac
     8161 catcgccgta cattattttc cagtatcctg gcgtcctttt taatgattac tggcagtacc
     8221 ggttacagcc atggattgga acccgtgagt ggtttattga cgccagaatc tgcggtgcaa
     8281 gctgctgatg gcaaaatcta tgtttccgaa atcaatggct ttggccaaga tggtgatggc
     8341 cagatccgcg tgatcgatca tggcaagtct tcagtattgg tacaggggct ggacgacccc
     8401 aaagggttgg tgattatagg aaatgattta tatattgctg ataaatcccg cgtactgcgt
     8461 attgccttaa atcaagagac ggctagtgcc gaagtattcc tggcagcgac agactttccc
     8521 aaagtaccac agttcttaaa tgacttgagt gcagatgcga atggcaatct gtatatcagc
     8581 gacagtggtg atatcttggg cacaggcaaa ggaggcgcca tttacaaagt cacacctcaa
     8641 cgcaaactca gcttgctgct cgatggcaat atcgacgcgc gcatcctggc ccccaatggc
     8701 ttgctggcgg atgccaaagg cgaacacttg ttactggtcg acttcacttc tggcgtactt
     8761 tacaactaca atcaagtgac caagaaactg gaagagcttg caaacggctt tggcggcgga
     8821 gatggtattg ttaagcaggc caatggcaat atttatgtca gtgactggaa aaatggcaaa
     8881 gtattccgcg ttgacccgca gcacaaagtc agtttaatta aagaaggtta tcagtctgca
     8941 gccgatattg ccctgacaca agatgaccat cacttgctgg tcccagacat gaaagctggc
     9001 aaattggaaa ttattgattt acgttgattt tggcagatgc gtagtcgata gataaaaaaa
     9061 accacgcttg cgcgtggtta agtgagagga tacacacgaa actgctgatt gcctcaacgc
     9121 actgcaggga attcagcacg cttaggcaac ttcacagtta cttggtttgc gcagtttttt
     9181 tgtcagcctc cttttggggg gtgaccactg gctgcgcgcc agcgtgtttg gctactttca
     9241 agtgtttttg catttcaacc tgatcatgct gcgaaggatt cgtccccgcc cagacacaga
     9301 ctgaaaaaat aattcctaaa atgagtaaca tggttttttt catgctcgct cctttactga
     9361 cgtgctcatt tctcttttat gattatttgg cattgcaggc atcatctgcc tacgccttgc
     9421 acgatacgcc catctcctta cagctaactt acgcctggct tgcagtaaac ttacaacgca
     9481 gaaaacccat aaacattgag ttacactggc agcattaaaa cccttttggt gaaagcaatg
     9541 cgtatattaa ttgtggaaga tgatttggtg attgcaaatg ggctggttcg tgcattggca
     9601 caatcacaat acgcagtgga tcatgtgaca gatggtaaac aggcgttacg cgccattcag
     9661 gatgacgtct acgacctggt catcctggac ctgggactcc ccaccatgga tggcctgcag
     9721 ttactatctc acctccgcca gcaagccaac ccgattgccg tactgatttt gactgcacgt
     9781 gaagatgtgg ccacccgcgt agaggccctt gatctgggcg cagacgactt cctgaccaag
     9841 ccatttcatc tggaagaact agaagcacgc gtacgcgcat tgatccgccg cagcaagctg
     9901 ggcaataact tgccattaca atgcggcaat ttgaaactgg atgtggccaa taagctggcc
     9961 agcattaatg aacagcctat taccctctcc gcacgagagc tgaatctgct ggaaacgctg
    10021 atgatgcgca tgggaaaaat catcgccaaa gagcagatca tggaatcctt atgcaactgg
    10081 gatgaaactc tgggtgacaa tgcgattgaa gtgtatgtcc accgtttgcg taagaaaatc
    10141 gaacccagtg gcgcacatat ccgtaccgta cgaggcctgg gttacatgtt ggaatccgag
    10201 cacctaaacc cttaaaatac cttaatgatg ggaccgcgtt cacttaaaaa ccaactccgc
    10261 ctatggctgt tactgacgat gctgctggta ttgggtgtct ctgcggctct ctcctactcc
    10321 cgcgcactgc attacgccac gcaagcttac gaccaagcct tattacgcac cgtgctcgcc
    10381 ctggctgatg aagtgattat tgatgccaac aatgaagtca aaatcgagat tcctgaagtc
    10441 gccagccact tattaagtta taacgagggt gaccgcattt acatacgcat cagtgcaccc
    10501 aatggtaaat tggtatttgg tgaagccaac ttgcctccac cagctcaggc accagcgggg
    10561 aaccagcagg tttattacaa tagccattac aaaaatgaag cgattagggc agtcactttt
    10621 ggcctgcctg aaagcaacgc ctcaaatgcg ctgaatatat tgatcaccat ggctgaaacg
    10681 actggcaaac gtgatgccat ggtcgccgag atggtggaag aaatgctgct accgcaagtg
    10741 ctgatgatga ttctggcggc cattgtcatt gaattaggca tacacttcgc actcaagcca
    10801 atgcaagacc tcagggaatc tttacaccaa cgctcccatc gtgacctcag cccgctggat
    10861 acgagcgctt cgccattgga gttgcaaccg ctgctacaag ccatgaatgc gctactgtcg
    10921 cgggtaaagg cagggataca acagcagcag caatttattg ccgatgcttc gcaccagctc
    10981 cgcacaccga tcgcaggctt gcagacccag gcggaattgg cactcagaag ccatccgcca
    11041 gcggaagtgc aggagccatt aaattacatg ctgcgtagca cgacgcgttt gtcgcatctt
    11101 attcaacgct tgctaagcct tgcccggaca gatgccgcca gtagccagct ggttttcaaa
    11161 ccggtttcac taaccagtat tatttatgac gaatgcgcac gctgggtggg tccggcatca
    11221 gacaaacaaa tagaactgga agtactgatt gaaactcagc aggaccaggt attaggcgat
    11281 gccttgatgc tttctgaaat gcttaacaac ttgctggaaa acgctatcca gtacacacct
    11341 gaacaaggct tgttaagaat tcgcctctgg caagcaggta aacatttatt actacaggtg
    11401 gctgacagcg gggtcggtat tccggccgac caaactgcct taattttcga acgtttccac
    11461 cggctggacc ccaaccaagg caatggttgt ggacttggcc tggccatcgt tgccgaaatc
    11521 gcagagcacc acaatgccag cattagcgtg gcacagggac tgcaacatcc aattacccac
    11581 cacttgggca gccaattcac ggtaaaattc gagcgctatg acaacctgcc tgagaaccct
    11641 cgcgctacaa gcactgacgc tcgatgaccc gcaacaaaaa tgtacgcaag tccagttatt
    11701 gtgggatgcc acattggcag gaacgctata cgtcaatgcc ccgctcagct tgcaaacacc
    11761 agaacatgcg attcctggtc gaccagcgaa gcctctccta gtcccaccca aacatgtcca
    11821 gcgtcgtgcc atgcataccg ccgaaggacg tgccgcgctc attcatgcgc tagcacatat
    11881 cgagtttaac gctatcaacc ttgcgcttga cgccgtctgg cgctttactg gcatgcctga
    11941 agattattac cgggactggt tacaggtggc caaagaagag gcttaccact tctcgctatt
    12001 gcgtgagcat ttgcaaagca tggggtttga ctatggtgat tttgatgccc ataacagcct
    12061 gtgggagatg gtagaaaaga cgcagtctga tgtattggca cgcatggctc ttgtgccccg
    12121 cacactggag gcacgtggat tggatgctac cccgccactc aaacaaaagt tgatgcaggc
    12181 gggtgatcac gccgcagctg cgattctgga tattattctg cgtgacgaaa tcggtcatgt
    12241 gaccataggc aaccgctggt tcaattttct ttgcgaccaa cgccacatca acccgatgca
    12301 tacatttgaa cagctttgtt tgcaatttaa agcgccaaaa ttaaaaccgc catttaatct
    12361 tgcagctcgc aaacaggcgg ggttcagtga gctcgaactt gcttatcttg atggcttaac
    12421 agcctgatcc cccgaaaaaa tattacagct gcatgttagg ttgagctgat
//
LOCUS       DATMSK010000028         6368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_378409,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000028 DATMSK010000000
VERSION     DATMSK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378409"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1128
                     /gene="mtnA"
                     /locus_tag="VNZ84_00940"
     CDS             61..1128
                     /gene="mtnA"
                     /locus_tag="VNZ84_00940"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HWU65544.1"
                     /translation="MRIHEQHFRTIWQEANAVCLIDQTRLPFEFVTVKVTTLPEICHA
                     IRSMQVRGAPLIGAAAAYGVALGIQETATDEHLGAVVSQLIATRPTAVNLAWAATRMQ
                     QQLKGLPVLARAAAAWQMAAAIADEDVAQNLAIGQHGLALIEAKATVNPRQAVNILTH
                     CNAGWLATVDGGTALAPIYAAHDAGLNVHVWVDETRPRNQGASLTAWELAQHGVPHTV
                     IADNAGGHLMQHGQVDMVIVGADRVSRHGDVCNKIGTYLKALAAFDQDIPFYAAVPTP
                     TIDWQLARGADIEIEMRDADEVAYMQGQAVDKQLHRIRVIPHESPALNPAFDVTPARL
                     VTGIITERGVFTPAQLRSLEP"
     gene            1130..1771
                     /locus_tag="VNZ84_00945"
     CDS             1130..1771
                     /locus_tag="VNZ84_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWU65545.1"
                     /translation="MTAPIPAEQLLQAAQKMAHHGLNKGTSGNASIRLDDGFLITPSG
                     VPAEQLTAGSMVYVDWQGVPEAGKKPSSEWRFHLDILLARQEVQAVVHCHSMFATTIA
                     CMGRHVPPFHYMIATVGGDDIRCAPYALFGTQALSDNAVTALQDRKACLLAHHGMISI
                     GNSLSQALAIAVEVENLCEQYWRLLQLDEPHLLTPEQMRDVHAQFKGYGQWGK"
     gene            complement(1776..2270)
                     /locus_tag="VNZ84_00950"
     CDS             complement(1776..2270)
                     /locus_tag="VNZ84_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65546.1"
                     /translation="MKHTPQSLAWQFCQGIYVNIVDAVLWQKTFEYIAQLEDDSAFQK
                     AKIATLWAHYGGDLRAKIRDDGLILHVLRKTMPSCRDDGRTLYRGESWFLFDGDQIGF
                     CWTPSQTIATSYAKGLNAVDSGGILLKCHAPAEAILAVENDVYICDPTHLLRMTTLEL
                     FPKP"
     gene            complement(2313..3041)
                     /locus_tag="VNZ84_00955"
     CDS             complement(2313..3041)
                     /locus_tag="VNZ84_00955"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HWU65547.1"
                     /translation="MTLPNDAPLQKLQFYVTTAYPCGYLPKKLAQSLIATPQNLVDGT
                     VYSGLIQQGFRRSGKFAYRPHCEHCNACISVRLPVNEFQSTRSQQRAFKKHAELQVSI
                     VEACYDEEHFALYKAYQAQRHHHGPDHPADQEDEAAQYQQFLCQSNVDSLMVEFRDAQ
                     YTLKMVSVIDIVMDGLSAVYTFYDANDKASYGTYSILWQIEWAKQLNLPYVYLGYWIA
                     NSQKMAYKQQFQPQEKLVDGEWIR"
     gene            complement(3028..3726)
                     /gene="aat"
                     /locus_tag="VNZ84_00960"
     CDS             complement(3028..3726)
                     /gene="aat"
                     /locus_tag="VNZ84_00960"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWU65548.1"
                     /translation="MHEQYYPLPEGPVLILGPETPFPPLQRALTEPNGLIAIGGDLSC
                     PRLLSAYTQGIFPWFSPGEPILWWSPDPRMVLFPQQLKINRSLRKVIQQQPFEIRFNT
                     SFKSVMEACAKTPRPGQPGTWISSEMIEAYQQLHHLGYAHSVEAWQHAQLVGGCYGVK
                     IGRMFYGESMFHHVSNASKVAFAHLVQWLNTQQVGMIDCQMHTPLLAGFGAYEIPRDR
                     FIAQLNELVQYDAT"
     gene            complement(3732..>6368)
                     /locus_tag="VNZ84_00965"
     CDS             complement(3732..>6368)
                     /locus_tag="VNZ84_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU65549.1"
                     /translation="LLVTARTLVSEHVLSSIALLKQYASVDGPPNIDGVTDMLGTTVP
                     NLRFGENAQTGRTILVDSVTRIGSGTATIFVKRGQQFLRIATNVKKADGSNAFGTELN
                     PYGKAIESLLKDQTYYGVVDILGEAYLSRYEPMHDEKGKLIGAWYVGYKLDFNALDQA
                     IRQWGFMEKGFVAITDGHGKIRFLTEGVSPSQALSALEDKQHQWKKVTKDVPEWDFQI
                     NILYPVKEAYWSALGAVSPVMIIAFSLTLIVVMVVISGLQRFVLNPLGGDPDTAKQLL
                     MRIESGDFAEDDTAAPPDTLIANMLKMRRRLREMVSQIQASANRLQVSASVFEHAHDA
                     IFITDSHGLIVQCNPSFSIVTGYFLADAIGQLPATLGIACQLPDFFQSLYTGPQAFQA
                     WKGEVWNRHQQGHDYLVELELSPVLGQAGEFQYYVGLFSDITHAKAQQNMLEHLAYHD
                     ALTQLPNRVLFSSRLQQALLEAERQQSLVAICYIDLDDFKIVNDQYGHDYGDRLLLQL
                     ATRLLGILKPQDTLARIGGDEFAMLLCGGRTQAEYTRLLKKLLAAIEKPFTVDKFKFS
                     ISASIGYTLYPIDHNPPDTLLRHADHAMYHAKTHGGHQYHLFDLASAQQSQQQQVLLR
                     DLLNAIKNNHIKLVYQPQICMQTGKLFCFEALLRWQHPIRGLLFPKDFLDAVEHTGLI
                     IDIGNWVIAESLRQLTEWQDHGLRTQVSVNIAAQHLMHKQFPDQLATLFKKFDRIQPA
                     QLHIEITESAAISDFDRVKRVIQQCQSLGVAFSIDDFGSGYSSLIYLRRLPVNIIKID
                     QSFVHNMLSNQEDMAVIRGVITLSREFGRKVVAEGVEKAEQARILADLGCDYAQGFGI
                     ARGMPGHKVIEWSSKHDPYHFS"
BASE COUNT         1513 a         1568 c         1711 g         1576 t
ORIGIN      
        1 aaaagcaagc ggttttacaa actttacaag aattattctc aattgcctgg catccctgat
       61 atgcgtattc atgaacaaca ttttcgtacc atttggcagg aagcgaatgc tgtctgcctg
      121 attgatcaaa cccggttacc ctttgagttt gtcaccgtga aggtgacgac attgccggag
      181 atttgtcatg caatccgtag catgcaagta cgcggtgcac ccttgattgg tgcagctgca
      241 gcttatgggg ttgcattagg catacaggaa acggctactg acgagcattt gggtgcagtg
      301 gtgagccagt tgattgccac caggcctacc gctgtcaact tggcttgggc ggcgacacgc
      361 atgcaacaac agttgaaagg attgccggta ttggcacgtg ccgctgccgc atggcaaatg
      421 gcggcggcca ttgctgatga agatgtggct cagaacctgg cgatcggcca gcatggactg
      481 gcattgatag aagcaaaagc gaccgtgaat ccacggcagg cggtcaatat cctgacccat
      541 tgcaatgccg ggtggttggc taccgtcgat ggtgggacag cgctcgcgcc catttacgca
      601 gcacacgatg ccgggctgaa tgtccatgta tgggtcgatg aaacgcgtcc gcgcaatcag
      661 ggcgccagcc tgacggcatg ggagctggca caacacggtg tgccgcacac ggtgatagcg
      721 gataatgcgg gagggcattt gatgcagcat gggcaagtcg atatggtgat tgtgggtgca
      781 gaccgggtat cccgtcatgg agatgtctgc aataagatag gtacctattt gaaagcgttg
      841 gccgcctttg accaggatat tcctttttac gcggcggtgc ctaccccgac gatagactgg
      901 caattggccc gtggtgccga tatcgagatt gaaatgcgtg atgctgatga agtggcctat
      961 atgcaaggcc aggcggtgga caagcagttg caccgcatcc gtgtgatccc gcatgaaagt
     1021 ccggcattaa atcctgcatt tgatgtaaca ccggcccggc tggttaccgg catcattacc
     1081 gaacggggtg tgtttacgcc tgcgcagcta cgatcactgg agccctgaca tgacagcccc
     1141 tatcccggca gaacagttac tgcaagcggc gcagaaaatg gcgcaccacg gactgaataa
     1201 aggcacttcc ggtaatgcca gcatccgttt ggatgatggc ttcttgatca cgccttccgg
     1261 ggtcccggct gaacagctca cagctggcag tatggtgtat gtggattggc agggtgtgcc
     1321 agaagccggt aaaaagcctt ccagcgagtg gcgtttccac cttgatattt tattggcgag
     1381 gcaggaggta caggctgtag tacattgcca cagcatgttt gcaacgacca ttgcctgtat
     1441 ggggcgtcat gttcccccat ttcattacat gatagcgacg gtgggcggtg atgatattcg
     1501 ttgcgcgccg tatgccttat ttggcacgca agccttgtcc gataatgctg ttactgcttt
     1561 gcaggaccgc aaggcttgcc tgttggcaca ccatggcatg atttccattg gcaatagcct
     1621 gtcgcaggcc ctggcaattg cggtagaagt tgaaaacctg tgcgaacaat attggcggtt
     1681 attacagttg gatgagccgc acttgttgac cccagagcaa atgcgtgacg tacatgccca
     1741 gttcaagggg tatggtcagt ggggaaaata aagggtcatg gcttgggaaa taattccagg
     1801 gtggtcatcc gcaacaggtg tgtcgggtca cagatataaa cgtcattctc gacagccagg
     1861 atggcttcgg ccggcgcgtg acacttaagc aaaatcccgc ctgaatcgac tgcattcagg
     1921 cccttggcat agctggtagc aatggtttgc gaaggcgtcc agcaaaagcc gatctggtca
     1981 ccgtcaaaca gaaaccagct ttcgccacgg tataaagtcc ggccatcatc acggcaagat
     2041 ggcatggttt ttctgagcac atgtaatatc agcccatcgt ccctgatttt tgcccgtaga
     2101 tcgccaccat agtgcgccca cagtgtggct attttggctt tttggaaagc cgaatcgtct
     2161 tcaagctgag cgatatattc aaaggttttc tgccatagca cggcatcaac gatattgacg
     2221 taaatcccct gacagaattg ccaggcgagg gattggggcg tgtgtttcat ggggatgtta
     2281 caaatgcatg gagatcgcag gcgtgtcagc cgttagcgga tccactcgcc atcgaccagt
     2341 ttttcctgcg gctggaattg ctgtttataa gccattttct ggctgttggc gatccagtaa
     2401 cccaagtata cataaggcag gtttagttgt ttggcccatt caatttgcca caagatgcta
     2461 taggtgccat aactggcttt atcgttagca tcgtaaaagg tatagaccgc agacaatccg
     2521 tccataacaa tgtcgatgac actgaccatt ttaagggtgt attgcgcatc ccgaaactca
     2581 accatcaggc tgtctacatt gctctggcac aagaactgtt gatattgtgc agcctcgtct
     2641 tcctggtcag ccggatgatc cgggccatga tggtgacgct gtgcttgata ggccttgtat
     2701 agtgcaaagt gctcttcgtc atagcaggct tcgacaatcg agacttgtaa ttcggcatgc
     2761 ttcttgaagg cacgctgttg gctgcgggtc gactggaatt cgttcacagg caggcgtacg
     2821 gaaatacagg cattgcaatg ttcacaatgc gggcggtagg caaacttgcc actacgtctg
     2881 aagccttgtt ggatcaatcc gctgtagacc gtgccatcca ccaggttttg cggcgtagca
     2941 atcaggcttt gtgccagttt cttgggcaga tagccacacg ggtaggcagt ggtgacataa
     3001 aactgcagtt tttgcagcgg ggcatcatta ggtagcgtca tattgcacta gctcgttcaa
     3061 ttgcgcgata aatcggtctc tcggaatttc atatgcacca aagcccgcca acagcggtgt
     3121 atgcatctga caatctatca taccgacttg ttgcgtgttt aaccattgca ccaggtgggc
     3181 aaatgcgacc ttggaggcat tgctcacatg gtgaaacatg ctttcaccat aaaacatacg
     3241 gccaattttg acaccgtagc agcctcccac cagctgcgca tgttgccagg cttctacgct
     3301 atgcgcgtaa cctagatgat gtaattgttg atacgcctca atcatttcgc tggaaatcca
     3361 ggtgcccggc tggccaggac gaggcgtttt cgcgcatgcc tccatcacgc ttttgaagct
     3421 ggtattaaat cgaatctcaa aaggttgttg ctgaatgact ttacgcaatg agcggttgat
     3481 cttgagttgt tgtggaaaca gcaccatgcg cggatcagga ctccaccaga gaataggctc
     3541 tcccgggctg aaccagggaa aaataccttg tgtataggca gagagcagcc tggggcaaga
     3601 aaggtcaccg ccaatcgcaa tcaatccatt gggttcggtg agggcccgct gcaagggtgg
     3661 aaacggcgtt tcaggaccca ggatgagtac tggtccctca ggcagagggt agtattgttc
     3721 atgcataggt gttaactaaa atgataaggg tcgtgcttgc ttgaccattc tataaccttg
     3781 tgaccaggca tgccacgcgc gatgccgaat ccttgggcat aatcacaacc cagatcagcc
     3841 aaaatacgcg cctgctctgc tttttctacc ccttcggcaa caaccttgcg gccaaattcg
     3901 cggctcaggg taatcacgcc ccggatcact gccatatctt cctggttact gagcatgttg
     3961 tgcacaaagg actggtctat cttgatgatg ttgaccggta ggcgacgcaa ataaatcaga
     4021 gaagaatagc ccgaaccaaa atcatcaatc gagaaggcga cacccagcga ttggcattgc
     4081 tggatcacac gcttgacgcg gtcaaagtca ctgatggctg cactttccgt aatctcgatg
     4141 tgcaattgtg ctggctgaat tcggtcgaat tttttaaaga gtgtcgccaa ttgatccggg
     4201 aattgtttgt gcatcaaatg ctgtgccgcg atattgaccg agacttgtgt gcgtagccca
     4261 tgatcttgcc attcggttaa ctggcgcaga ctttctgcaa tcacccagtt tccgatatca
     4321 ataatcaggc cggtgtgctc aacggcgtcc agaaagtctt tggggaaaag caggccacgt
     4381 atcgggtgtt gccagcggag caaggcttca aagcaaaaca gcttgcctgt ctgcatgcat
     4441 atctgtggct ggtaaaccag cttgatatga ttgtttttga tcgcgttgag caagtctcgc
     4501 agaagcactt gctgttgttg cgactgttgt gccgatgcca ggtcaaacag gtggtattgg
     4561 tgaccaccat gtgttttggc gtgatacatc gcatggtcgg cgtgacgcag caacgtgtct
     4621 ggcggattgt gatcgatcgg atacagggtg tagccaatac tggcagaaat cgagaatttg
     4681 aatttatcga ctgtaaacgg cttttcaatc gcagccagca attttttgag caaacgcgtg
     4741 tattcagcct gtgtgcgacc accgcaaagc aacatggcaa attcatcacc acctattcga
     4801 gccagtgtgt cctgcggttt gagtatgcct agcagcctgg ttgccagttg caaaagcaac
     4861 ctgtcaccat aatcatggcc atattggtca ttcacaattt tgaagtcgtc gagatcaata
     4921 taacaaatcg cgaccagtga ttgctggcgc tccgcttcca gtaaggcttg ctgcaagcgg
     4981 ctggagaaca gtacgcggtt tggcagttgc gtgagtgcat cgtggtatgc caggtgctcc
     5041 agcatgtttt gctgcgcttt ggcatgcgtg atgtccgaaa acaacccgac ataatattga
     5101 aactcacccg cctgaccgag taccggtgac agttccaatt cgaccaggta atcatgtcct
     5161 tgctggtggc gattccatac ttcccctttc catgcctgaa aagcctgcgg gccggtgtac
     5221 agggattgaa aaaagtccgg tagctgacag gcaatgccca gtgttgcggg taactggcca
     5281 atggcatcgg ccaggaagta gccggtcaca atactaaagg aaggattgca ttgcacaatc
     5341 aggccatggg aatcggtaat gaagatggca tcatgtgcgt gttcaaacac gctggcagaa
     5401 acttgcagcc gattggcgct ggcttggatt tggctcacca tctcacgcag tcgccgccgc
     5461 atttttaaca tattggcgat cagtgtatcg ggcggtgctg ccgtatcatc ctctgcaaag
     5521 tcgccacttt ctatacgcat gagtagctgt ttcgccgtgt ccggatcgcc gccaagcgga
     5581 tttaacacaa aacgttgcag accgctgatc acgaccatga cgacaatcag ggtcagactg
     5641 aaagcgataa tcatcacagg tgaaactgca cctaacgctg accagtaagc ttccttgacc
     5701 gggtacagga tattaatctg gaaatcccat tccggcacat ccttggtcac tttcttccat
     5761 tgatgctgtt tgtcttccag tgcggacaac gcttgtgatg gactgacgcc ctctgtcagg
     5821 aaacggattt tcccgtgacc atccgtgatg gcgacaaagc ctttttccat gaatccccat
     5881 tgacggatgg cttggtccag ggcattgaaa tcgagcttat agcccacgta ccaggcgcca
     5941 atcaatttgc ctttttcgtc atgcatgggt tcgtagcgcg agaggtaagc ctctccaaga
     6001 atatctacca caccgtaata cgtttggtcc ttgagcaggc tttcaatcgc cttgccgtaa
     6061 gggtttaatt ctgtaccaaa tgcattactg ccatccgctt ttttgacatt ggtggcaata
     6121 cgcaaaaact gctgaccgcg tttgacaaaa atagtggccg tgccgctgcc aatacgggtg
     6181 accgaatcta ccagaatggt gcggccggtt tgtgcatttt caccaaagcg caggttgggg
     6241 acggtagtcc ccagcatatc tgtcacgccg tcgatgttgg gtggtccatc caccgaagca
     6301 tattgtttga gtaaggcaat actggagagc acatgttcac tgaccagcgt tctggcggtg
     6361 actagcag
//
LOCUS       DATMSK010000029         5530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_120067,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000029 DATMSK010000000
VERSION     DATMSK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120067"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /gene="flgL"
                     /locus_tag="VNZ84_00970"
     CDS             <1..802
                     /gene="flgL"
                     /locus_tag="VNZ84_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HWU65550.1"
                     /translation="VAAARLLELNTAKDLNANFGSTRKTAETLLQTYETSLSNVTDTI
                     QAIQSSVIAAGNGTYTDSERTKVANELQGYMDTLLNLANSKDSQGNYMYAGYATQTVP
                     FELDTSTTPNRYVYKANSTRTELQVDIQSKMSVTYTGDQVFGPDGANVFKTLQDMITL
                     LKTPITDSTTQATYSAGVASTIDQMQGWLNNVLDARSEIGSNLNFIDTMNTTGDSLDY
                     QYDASISELQDLDYAQALSDISKKTSILQAAQKAFVATSNISLFSLIS"
     gene            complement(855..1622)
                     /gene="fliR"
                     /locus_tag="VNZ84_00975"
     CDS             complement(855..1622)
                     /gene="fliR"
                     /locus_tag="VNZ84_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HWU65551.1"
                     /translation="MLNIHASDLQLWIVQLLWPLTRILGCLTVVPLFSHPAIPKRIKL
                     AIGFVITLAIVPSIQVPAIEVFSWQGMLSLLAQFLIGISMGFVMRLLFTAVEMAGFMM
                     GMSMGLGFASFYDPQAHGQTIAVGQFLTMLALLVFMSLDGHLIVVAVMLQSFETMPIG
                     LEHWQLNSQSIATLGGHIFSQGLMLSLPVVASLLITNMALGILTKAAPQFNIFGIGFP
                     ITLSVGFLMTLLSLPSMVSPISRWLVDAQQLMLQVLH"
     gene            complement(1638..1907)
                     /gene="fliQ"
                     /locus_tag="VNZ84_00980"
     CDS             complement(1638..1907)
                     /gene="fliQ"
                     /locus_tag="VNZ84_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HWU65552.1"
                     /translation="MSPEQVVSLGSEAMQTTLMVAAPVLGTALIIGLIVSIFQAATQI
                     NEQTLSFIPKLVGVMAVLLLAGHWMLMTMVDYMHHVFTMIPQMVN"
     gene            complement(1939..2706)
                     /gene="fliP"
                     /locus_tag="VNZ84_00985"
     CDS             complement(1939..2706)
                     /gene="fliP"
                     /locus_tag="VNZ84_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882539.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HWU65553.1"
                     /translation="MRVFSVLKSIFSLLLPVALLWAVPQWSLAAEGLPIGTVTPAAGG
                     GQDYTLSLQTLILLTSLTFIPAAMLMMTGFTRIVIVLSLLRQALGTQSAPSNQVLIGL
                     ALFITFFVMGPVFDKIYTDAYQPLSESKITMQEALQRGVLPLKGFMLKQTRESDLAMF
                     ANMAHTETIQTPDEVPLRILVPAFMTSELKTAFQIGFAIYIPFLIIDMVVASILMAMG
                     MMMMSPSIVSLPFKLMLFVLVDGWQLILGSLAQSFVI"
     gene            complement(2820..3332)
                     /gene="fliO"
                     /locus_tag="VNZ84_00990"
     CDS             complement(2820..3332)
                     /gene="fliO"
                     /locus_tag="VNZ84_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HWU65554.1"
                     /translation="MSYCSKLLFALFCSMMTTGVAWAAAPAVAAAAVPTPGDSLGRMV
                     FGTVIVLLVIAAIAWLLRRVMPGQTMAQRGVIKQVGGLQLSPRERVVVLEVAGRWLVV
                     GINAGQMTALGEVDPMVTETKATETPAEEGATLSTVSGIDPEASFAVRLQQAMQATLS
                     QTVKSFKNKP"
     gene            complement(3355..3789)
                     /gene="fliN"
                     /locus_tag="VNZ84_00995"
     CDS             complement(3355..3789)
                     /gene="fliN"
                     /locus_tag="VNZ84_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HWU65555.1"
                     /translation="MAAMGVSDPGDVQTIAEDDWGAALAEQTQAESSALQEQTSKTAV
                     FNEFSANNKLEGTQNDIDFILDIPVQLTVELGRTKISIKNLLQLAQGSVVELDGMAGE
                     PMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRKLNR"
     gene            complement(3840..4841)
                     /gene="fliM"
                     /locus_tag="VNZ84_01000"
     CDS             complement(3840..4841)
                     /gene="fliM"
                     /locus_tag="VNZ84_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HWU65556.1"
                     /translation="MSADKFLSQDEIDALLKGVEGDDDSDSSGKDEAGVRTYNLATQE
                     RIVRGRMATLEMINERYARLLRVELFNFLRRTVEVSVGPVRIIKYTDFIRNLVVPTNL
                     NLIHVSPLRGTGVLVLDPTLVFLVVDNMFGGDGRFHTRVEGRDFTATEQRIIQRILNI
                     MFEAYEKSWAPVYPVKFEYLRSEMNTQFANIATPNEVVVAITFNIELGPNSGEMHFCV
                     PYSMVEPIKDLLTSPLQGENLGGDKRWVKLMTQQIQAAEVEIVANLATRKMTVADLLE
                     LKEGDVIPINISDSIEGQIDNIPVMDCRYGVFNGQYALKVEKLIRADGSQEQLQGES"
     gene            complement(4873..5460)
                     /locus_tag="VNZ84_01005"
     CDS             complement(4873..5460)
                     /locus_tag="VNZ84_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HWU65557.1"
                     /translation="MAQEPAKAEGAAEAAPKSKKKLIVILAVVLALAAGGGAAAFFLM
                     KKPEAHQAKGKHGDKAEKAEGADDEAISEEAADEGEEESGGHADPKTAPTYVQFETFT
                     VNLLPDPDEKFLQLDLTIEVKGAELAEKLKAQMPALRNRVLLLLTSKKASDISTPEGK
                     AQLSAELLAELKKPLTAHGKPVKVNQVLFTSFVIQ"
BASE COUNT         1490 a         1467 c         1268 g         1305 t
ORIGIN      
        1 tgtcgctgcg gccagattat tagagctcaa caccgcaaaa gatctgaacg cgaactttgg
       61 tagcacgcga aaaacagcag aaacattgct gcaaacttac gaaaccagtc tttctaatgt
      121 cactgatacc attcaagcca ttcagtcatc cgtgattgca gcaggaaacg gcacatacac
      181 agattcagaa cgtacaaaag tggctaatga gttgcaaggt tacatggata cgttattgaa
      241 cctggctaac agtaaagatt cccagggtaa ttacatgtac gcaggctatg cgacgcaaac
      301 agtgcctttt gaattagata cgtcaaccac tcccaatcgt tatgtataca aagcgaattc
      361 aacgcggaca gaattacagg tagacataca aagtaaaatg agtgtgacct acacagggga
      421 ccaagttttt gggccagatg gcgcaaatgt ctttaaaacc ctgcaggaca tgatcacgtt
      481 attgaagact cccattacag acagcacgac acaagccact tattctgctg gtgtcgcctc
      541 tacgattgat caaatgcagg gttggttgaa taatgtactg gatgcgcgct cggaaattgg
      601 tagcaatctt aattttattg ataccatgaa caccacgggc gacagtctgg actatcagta
      661 tgatgcctct atttctgagt tgcaggattt ggattatgct caggcgttat ctgacatctc
      721 caagaaaaca tcgatactgc aggcagcgca aaaagccttt gtcgccacat caaacatctc
      781 gctatttagc ttgatttcct aatagacaga ggccgcgcaa agaactggcg cggcccttag
      841 atgcttctct ctgattaatg cagcacttgc aacataagct gctgagcatc caccagccat
      901 cggctgatag ggctgaccat tgaaggcagg cttaacagtg tcatcaaaaa acccaccgaa
      961 agcgtgatcg ggaacccaat accgaaaatg ttgaattgtg gcgcggcctt ggtcaaaatg
     1021 cctagcgcca tgttggtgat caacaggctg gcaaccacgg gtaatgacaa catcaatcct
     1081 tgcgaaaaga tatgcccacc cagtgtggcg atggactggc tattgagttg ccagtgctca
     1141 agcccgatgg gcatggtttc aaaactctgc agcatcacag ccaccacgat caggtgacca
     1201 tccaggctca taaacaccaa tagcgccagc atcgttaaaa actggccaac ggcaatggtt
     1261 tgcccatgcg cttgcggatc ataaaagctg gcgaagccta gccccatact catacccatc
     1321 ataaaaccag ccatttccac cgcagtaaac agcaggcgca tgacaaagcc catactgatc
     1381 ccaatcagga attgtgccag caaggaaagc atcccttgcc aggaaaatac ctcaatggcc
     1441 gggacctgga tagaagggac aatagcgagg gtaatgacga agcctatcgc aagcttgata
     1501 cgtttgggaa tggccggatg gctaaatagt ggaacgactg tcaggcaacc taatatgcgc
     1561 gtcagtggcc acagcagctg aacgatccat aattgcagat ctgaagcatg gatattcagc
     1621 atcgcgtgtg caacccctta gttcaccatt tggggaatca tggtaaacac atggtgcata
     1681 taatccacca tggtcatcag catccagtgg cctgccaaca ataacacagc catcactcct
     1741 accagtttgg ggatgaatga caatgtctgt tcattgattt gcgtggccgc ttggaaaata
     1801 ctgacaatca aaccgatgat cagcgcggtc cctaatactg gcgctgccac catgagcgtg
     1861 gtttgcatcg cctcgctgcc caatgaaaca acttgttctg gactcatggt ctactcctga
     1921 aattctaaat aagaggattt aaataacaaa gctttgcgcc aacgaaccta gtatcagttg
     1981 ccagccatcg accaggacaa acaacatgag cttgaacggc agcgacacaa tagagggaga
     2041 catcatcatc atccccatgg ccatgaggat actggcgacc accatgtcga taattaaaaa
     2101 cgggatataa atggcaaagc cgatctggaa agcagttttc agctcactgg tcataaatgc
     2161 cggcaccaat atacgcaatg gcacctcatc tggcgtttga attgtttcgg tatgggccat
     2221 gttggcaaac atggccaaat cactctcacg tgtttgcttg agcatgaaac cttttagcgg
     2281 caacacgcct cgctgcaatg cctcctgcat ggtgatcttg ctttcagaca agggctggta
     2341 agcgtcggta taaattttgt cgaataccgg tcccatcaca aaaaatgtaa tgaacagtgc
     2401 caaaccaatc agcacctggt tggaaggcgc agactgcgtg ccgagtgctt gcctgagcaa
     2461 ggacaacaca atcacgattc tggtaaagcc cgtcatcatg agcatggctg ccgggataaa
     2521 tgtcagtgaa gtaagaagga tcagcgtctg caggctgagc gtatagtctt gcccaccacc
     2581 tgccgccggg gtcactgtgc caatagggag gccttctgct gccaaagacc attgtggcac
     2641 agcccatagc agggcaaccg gcaacaacaa agagaaaata gactttaaaa ctgaaaatac
     2701 ccgcatgtac atggcacccg aaacttgctt gtcctgccgt gaggccaggc acaagtccat
     2761 caataagtag ggttatagta cgctagggca gattaaatca aaggttggaa atgaacgact
     2821 caaggcttgt ttttgaagga tttgacagtt tgggacaatg tcgcctgcat ggcttgctgc
     2881 aagcgtacag caaaagaagc ctccggatcg atcccgctca ctgtagaaag cgtagcgccc
     2941 tcttctgctg gcgtttcagt ggcctttgtt tcagtgacca tagggtcaac ttcaccgagg
     3001 gcggtcattt gcccggcatt aatcccaacc accagccatc ggccagccac ctccaggacg
     3061 actacgcgct ccctggggct aagctgcaaa ccacccactt gtttgatcac gccacgttgt
     3121 gccatggttt gtcctggcat gacacgccgt aacagccagg caatcgcggc aatcaccagc
     3181 aagacaatca cagtaccaaa caccatgcgg cccaggctgt ccccaggagt cggcacggca
     3241 gcagcggcca cagcaggcgc agctgcccaa gccaccccag tggtcatcat gctgcaaaat
     3301 aaggcaaaca acagcttgct gcaataactc atgacaccct aatcagaaat cacattaacg
     3361 gttcaatttg cggatacgct cggctggcgt aatgatatcg gtcaggcgga taccaaactt
     3421 gtcattgacc actaccacct cgccttgggc aatcaggcag ccattcacga gtacatccat
     3481 aggctcaccg gccataccat ccagctctac cacggagccc tgggccaatt gcaacaaatt
     3541 cttgatcgag attttggtgc gccctaactc taccgtcaac tgtaccggga tatccaggat
     3601 gaaatcaata tcattctgtg tcccttccag cttgttgtta gcactaaact cgttaaacac
     3661 agcggttttg ctggtttgct cttgcagcgc agaagactca gcctgggttt gttcagcgag
     3721 cgccgcaccc cagtcatcct ccgcgatggt ctgtacatcc ccgggatcag acactcccat
     3781 ggcagccatc gctgcttcca tatcatcatc tgcaccgtta gtggcgggat ccatagccat
     3841 tatgactctc cttgtaattg ctcctgacta ccgtcagcgc gaattaactt ttcaaccttg
     3901 agggcatatt gcccgttaaa cactccataa cgacaatcca tgactggaat gttgtcaatc
     3961 tgtccttcaa tactgtcgct gatattaatc gggatcacat ccccttcctt cagttccagc
     4021 agatcagcca ccgtcatctt gcgggtagcc aggtttgcaa cgatctcgac ttctgccgcc
     4081 tggatctgtt gggtcatcaa tttgacccag cgcttatcac cccccaggtt ttcaccttgt
     4141 aatggggatg tcagcaagtc tttaatcggc tccaccatgg aatatggcac gcagaaatgc
     4201 atttcaccgc tattagggcc tagctcaata ttgaatgtga tggcgacgac cacttcattg
     4261 ggcgtagcaa tattggcgaa ctgcgtattc atttccgaac gcaaatactc gaacttgacc
     4321 gggtacacag gtgcccagga cttttcgtat gcctcaaaca taatattgag gatgcgctgg
     4381 ataatccgtt gttcagtagc cgtaaaatca cgcccttcaa cacgggtatg aaagcggcca
     4441 tcgccaccga acatattgtc taccaccagg aatacaagcg tagggtccag cacaagcaca
     4501 cccgtaccgc gcaagggact cacatgaatc aagttaagat tggtcggtac gaccaggtta
     4561 cggatgaagt ccgtatactt gatgatcctc acagggccga ctgacacttc gaccgtgcga
     4621 cgtaaaaagt taaataactc tacacgcagt aatcgcgcat aacgctcatt aatcatctcc
     4681 agcgtggcca tacggccgcg cacgatgcgc tcttgcgtcg ccaggttata ggtgcggaca
     4741 ccagcttcgt ccttaccaga cgaatcactg tcgtcatcgc cctcaacacc cttgagcagc
     4801 gcatcaattt cgtcttgtga taaaaacttg tcagcggaca tgaggattca ctttacacaa
     4861 caggctttgc cattactgga tcacgaagga ggtaaacaat acctgattaa ctttgaccgg
     4921 tttgccatgg gccgttaatg gctttttcaa ttctgccagc aactccgcac ttagctgtgc
     4981 tttgccttct ggcgtagaaa tatcagaggc ttttttgctg gtcagcaata gcaatacgcg
     5041 gttacgtaag gcaggcattt gcgctttcaa cttctcggcc agctcggctc cttttacttc
     5101 aatggtcaaa tccagttgca agaatttttc atccggatct ggcaacaggt tgacggtaaa
     5161 tgtttcaaac tgcacataag tcggtgcggt tttagggtca gcatgaccac cagattcctc
     5221 ttcgccttca tcggcagcct cttcgctgat cgcttcatca tcggcacctt cggctttttc
     5281 agctttatca ccatgcttac ccttggcttg atgcgcctcg ggtttcttca tcaggaaaaa
     5341 agcagcagcg ccgccaccag cagccagtgc cagtacaacg gccaatatca caatcagttt
     5401 tttcttggat ttgggtgcag cctctgctgc gccttctgct tttgctggtt cttgagccat
     5461 gacacaaacc tttcttacgt gaaaccttaa gtgtttatga tgacattatg cgcaaaactg
     5521 cggtttggag
//
LOCUS       DATMSK010000030        10325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_96410,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000030 DATMSK010000000
VERSION     DATMSK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96410"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VNZ84_01010"
     CDS             complement(<1..266)
                     /locus_tag="VNZ84_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWU65558.1"
                     /translation="MFEITKKSIPFGAHTLTLETGEIARQSDAAVIVSYGDTVVLVAV
                     TAKREVKEGQDFFPLTVDYQEKTYAAGRIPGGFFKREGRPSEKET"
     gene            complement(583..852)
                     /gene="rpsO"
                     /locus_tag="VNZ84_01015"
     CDS             complement(583..852)
                     /gene="rpsO"
                     /locus_tag="VNZ84_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWU65559.1"
                     /translation="MAITAEQSAKIIKEYQRSANDTASPEVQVALLTFRITYLTEHFK
                     ANKKDNHSRRGLLALVSQRRRLLDYLKGKDVSRYQTLIERLNLRK"
     gene            complement(1033..1926)
                     /locus_tag="VNZ84_01020"
     CDS             complement(1033..1926)
                     /locus_tag="VNZ84_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWU65560.1"
                     /translation="MSNSIMILGEVLGDVFPEQTIIGGAPYNVARHCHAFGLDVHLLS
                     AVGKDALGQRILAEMRDFGLPTGGVQESATLPTGQVMVHFEKEGHRFEILDQQAYDDM
                     QWPPVEALTVKHPPRLVYFGTLALRHAAMQSLAAQWLKQCEAATVFCDINLRAPWYDE
                     ASVRLVLQASHILKINHEELPEVCQLLGLPKSPDINDQARHLLMAFNLREMFVTCGEQ
                     GSFWLNDYGDGFRAPPIRLQSPLVDSVGAGDAYTSIVIRGLLAGWSRQTILPRAAAFA
                     ASICGIRGAVPDDLEFYKDFI"
     gene            complement(1931..3259)
                     /gene="chrA"
                     /locus_tag="VNZ84_01025"
     CDS             complement(1931..3259)
                     /gene="chrA"
                     /locus_tag="VNZ84_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HWU65561.1"
                     /translation="MSASNITQAQTAPVRPTPAVFWRYWFRLGCISVGGPAAQIALMH
                     QDLVVEKRWISDARFLHALNYCMVLPGPEAQQMAVYMGWLLYGVWGGIVAGSLFILPA
                     FALLVALSWMYMQWGSQPEVVALLYGVKPLVVALIFSAAWRLAKRVLHASWLWALFLL
                     ALCLHVLDVPFPTIILLAGVVGWLMQQYWPMRVQPQTSHSHVASTSHLPCVIDDHTSP
                     AVIGPIQPLIVWTLGIAALLGAGGLMGLVLCYGWPHVFSQLALFFSKAAFLTFGGAYA
                     VLPYVFDASVQHFHWLNALQMLDGLALGESTPGPLIMVVTYIGFVAGWQAGLPMPTWQ
                     AGLIGAGVVTWFTFLPSFALVFLGAPWMERARQQWRWAAPLHAISAAVVGGIVALATT
                     LGKHVVWPAGLDWVAAGMVLLALWLLLKMRVSILWLLPGFMALGWLFKLG"
     gene            3399..3474
                     /locus_tag="VNZ84_01030"
     tRNA            3399..3474
                     /locus_tag="VNZ84_01030"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3432..3434,aa:Phe,seq:gaa)
     gene            3517..3834
                     /locus_tag="VNZ84_01035"
     CDS             3517..3834
                     /locus_tag="VNZ84_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWU65562.1"
                     /translation="MSLKHLTPDELHPLLSDPSLLLVDIRNDHELVSGILPGALHVPL
                     GNLAAVFDQFPQDKKIVFYCRSGVRSVAAGEFALAQGRTDVSHLAGGILAWANAGMPI
                     QAA"
     gene            3855..4088
                     /locus_tag="VNZ84_01040"
     CDS             3855..4088
                     /locus_tag="VNZ84_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWU65563.1"
                     /translation="MQFDKEVDARNLNCPLPILRCKKALNELAAEQVLKITATDPGSK
                     KDFDAFCRQTGHTLLDMQEAEGVFTFLIRKRSQ"
     gene            complement(4445..5575)
                     /locus_tag="VNZ84_01045"
     CDS             complement(4445..5575)
                     /locus_tag="VNZ84_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65564.1"
                     /translation="MQMLNSPLARRTRMSFLLLALVATVSACNKQEEPAAEAAAPATE
                     SAAGAVAYVSTQDAGVNVIDLATMQVTKQFDVKAQGPRGLAVTDDGKQLVVATRENGS
                     VSVIDTATGEVVKQIEVGKNPEFVRVSGNYAFVSSEPSAKAGPPPKPGAKPEEDDDDD
                     DEEKIPAKIAVIDLSKGEKIREITGGPETEGLEFSADGKQLVITNEADNTVTVHNIET
                     GELVKTIPTHEYGDRPRGIKVSPDGNTYLATLEFGNKFMVMDKDFNVVRTVDTAETPY
                     GISYDSKGERIFVATNKAKLLQVFDAKGFEKIKEVPTGNRCWHFSFTPDNKNVLLACG
                     KSDAVFVIDADKLEVTNQVEVKNMPWGVVTFPKAMGSLESVK"
     gene            complement(5715..7226)
                     /gene="ilvA"
                     /locus_tag="VNZ84_01050"
     CDS             complement(5715..7226)
                     /gene="ilvA"
                     /locus_tag="VNZ84_01050"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HWU65565.1"
                     /translation="MTINYREKIEHSQVYAVARHTPLDEMPNLSARFKNRILLKREDM
                     QPVFSFKLRGAYNKMANLSPEALARGVICASAGNHAQGVALSAQKMGCRAVIVMPTTT
                     PAIKVNAVRSRGAEVVLIGDSYSDAYAHALEVEKNEGLTFVHPYDDPDVIAGQGTIAL
                     EVVQDHPEPIEAIFCCVGGGGLLAGIAAYIKAVRPEIKVIGVEAQDAEAMTESLKTGQ
                     RVMLEQVGLFADGAAVKQVGEHTFALVQQYVDEMIVVDNDEVCAAIKDVFEDTRSILE
                     PAGALALAGLKAYSEKYQLQGKTMIAVASGANMNFDRLRFIAERAELGEHREAVFAVT
                     IPETPGAFKAFCRLLGNRSITEFNYRFSDAKEAHIFVGIAVHNPAEAAEIASKLEQAG
                     LPTLNLSDNEMAKLHLRHLVGGHAPQAQNEVVYRFEFPEKPGALMNFLESMGHNWNIS
                     LFHYRNHGADFGRVLVGMQVPPEDKVAFAQFLQQLGYPCWDESQNPAYRLFLG"
     gene            7322..7984
                     /gene="rpiA"
                     /locus_tag="VNZ84_01055"
     CDS             7322..7984
                     /gene="rpiA"
                     /locus_tag="VNZ84_01055"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HWU65566.1"
                     /translation="MDKAQQDALKLEVAKAAVTYVKDGIIGVGTGSTANFFIDELAKV
                     KHKITGAVASSEATAQRLRNHGIEVFDLNSVDSLDIYVDGADEITEHMHMLKGGGGAL
                     TREKIVAAVAKSFICICDESKFVPVLGKFPLPVEVLPMARSHVARELVKLGGQPQLRD
                     FTTDNGNLILDVHGLTISDPIDTEAKINQIVGVVTNGLFAARPANVLLLATPNGVKTL
                     TK"
     gene            8072..8776
                     /gene="phoU"
                     /locus_tag="VNZ84_01060"
     CDS             8072..8776
                     /gene="phoU"
                     /locus_tag="VNZ84_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWU65567.1"
                     /translation="MQFEHTSKQYDVELESVRAKVLEMGGLVEQQIVNALEALTSADV
                     NLAKDVMERDTRVNALEVQVDEDCSHIIARRQPAARDLRMIMMMVKTITDLERIGDEA
                     TKIARTAQRIYEQDRMYKPRFNEIKSMVAIVREMLRTSLDSFARLDISQTVEVAKQDE
                     QVDDQFRAAMRQLITFMLEDPRTISMSLEVLFVAKAIERIGDHAKNISEYVVYMVKGK
                     DIRHTSLEDIQRETQS"
     gene            complement(8840..9112)
                     /locus_tag="VNZ84_01065"
     CDS             complement(8840..9112)
                     /locus_tag="VNZ84_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HWU65568.1"
                     /translation="MARMVQCVKLGKELEGMDFPPYPGELGKKIYMHVSKEAWAGWLK
                     QQTMLVNENRLSLADPSARKYLAEQTEKYFFGDGADVASGYVPPQS"
     gene            9190..9585
                     /locus_tag="VNZ84_01070"
     CDS             9190..9585
                     /locus_tag="VNZ84_01070"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HWU65569.1"
                     /translation="MQNNQSSSHQESPFKGKTGIRRLINAFGYSMDGFKAAYQHEDAF
                     RQEVWLSLVLIPLAFYLETEALHRILMVGSVLLVMMVELLNSAVEAVVDRVSIERHAL
                     AKRAKDIGSAAVLLALINLGVVWGLILLG"
     gene            complement(9673..>10325)
                     /locus_tag="VNZ84_01075"
     CDS             complement(9673..>10325)
                     /locus_tag="VNZ84_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWU65570.1"
                     /translation="LYANGEHVATGQFEVGNANLNKEISNGLDAQLKWKENGHSITLG
                     AYATRFQNFIGLFNSGATDPGTGLAIANFAAVPALFKGLELEGKFALNDEWTLKMRGD
                     YVHAKDTRNNQYLPRISPLRLGGGLDYRLGNWNARVDVLHAFKQNDVAENELKTNGYT
                     NLSALVAYKLPVKYHVELFAKANNLLNDEIREHASFLKDISPAGARSVLVGARADF"
BASE COUNT         2656 a         2832 c         2373 g         2464 t
ORIGIN      
        1 gtttcttttt cagaaggacg gccttcacgt ttgaagaagc cacctggaat acggccagcc
       61 gcataggttt tttcttgata atcaacagtt aatggaaaga aatcctggcc ctctttcact
      121 tcgcgcttgg ccgtcacagc caccagcaca accgtatcgc catagctaac gatcactgca
      181 gcatcagatt ggcgtgcaat ttcacctgtt tccaatgtca gggtatgcgc accaaacggg
      241 atgctttttt tagtaatttc aaacatattt ttccttttct ctaccatggc tattcactac
      301 ctaatagcca atctcaataa tgaaaacaca gcattcgatg attgcggcta agcgatattc
      361 attgccttgc ctaacgcgca accgctgtat ttcctgatgt atggtgactt ccatcaacac
      421 gctgcatcta tggaaccagt tgcctgaccc agttacaacg cactcacaga agccaccgcg
      481 ttttggtgat tttttaaagc aaaaaagccg aggcaatcac catgccatcg gcttttatct
      541 cgttaaaagt gaacaggatt aagtgtcaca ctttcaacca aattatttac gcaggttcaa
      601 gcgctcgatc agcgtttgat agcggctaac atctttaccc ttgaggtagt ccagcaaacg
      661 acgacgctgg ctaaccaatg ccaacagacc acggcgtgag tggttgtctt ttttgttggc
      721 tttgaagtgc tcggtcaggt aagtgatacg gaatgtcaac aaagccactt gtacttctgg
      781 gcttgctgta tcgttagcac tacgttgata ttctttaatg atttttgcgg attgttctgc
      841 agtaatcgcc atctttattt tccttcagtt agcgtcaatg tctcagtagc caagcaaccc
      901 atttgctcac cacaccgaca ttcaatttac atgaaccgcg cattatagcg gcaatgccaa
      961 tacttgcaaa ggcattttta gcattaagtt tctgtttttg cttatttttt gccagccttg
     1021 cgcagggaag cgctaaatga agtctttgta aaactcaaga tcatcaggca ctgcgccacg
     1081 gatgccacag atagaggcag cgaacgctgc ggcgcgtggc aagatggttt gccgtgacca
     1141 gccagccagt aacccacgaa tcacaataga cgtatatgca tcaccggcgc ctacgctatc
     1201 caccagcggt gactgtagcc gtatcggcgg tgcccgaaaa ccatcaccgt aatcgttcag
     1261 ccagaaactg ccttgctcac cacaggtgac aaacatttcg cgaagattaa atgccatcag
     1321 caaatgcctg gcttggtcat tgatatccgg cgacttgggc aatcctaata actgacatac
     1381 ttccggcaat tcttcatggt taatcttgag aatatgtgag gcttgtaaca ctaggcgcac
     1441 actggcttcg tcataccatg gcgcccgcaa gttgatatcg cagaacaccg tggcagcctc
     1501 acattgcttt agccattgcg ccgccagcga ctgcatggcc gcatgccgga gggcaagcgt
     1561 gccaaaatat accagcctgg gcggatgctt gacggtgagc gcctccactg gcggccactg
     1621 catatcatcg taggcttgct ggtcaaggat ctcgaaacga tgcccctctt tttcaaaatg
     1681 caccatcacc tgcccggtcg gcaaggtcgc tgactcttgt acccctccgg taggcaaacc
     1741 aaaatcacgc atctctgcca agatacgttg acccagcgcg tccttaccga cagccgagag
     1801 caaatgcacg tccaggccaa acgcatggca gtgcctggcc acattgtatg gcgcaccacc
     1861 gatgatggtt tgttcaggga agacatcccc caatacctcg cccagaatca tgatggaatt
     1921 cgacataccc ttatcctaat ttgaataacc aacccaatgc cataaaccct ggtaacaacc
     1981 aaagaataga cacacgcatt tttaacagca accaaagtgc cagcaacacc attcccgctg
     2041 ccacccagtc aagccctgcg ggccacacaa catgcttacc aagagtggtc gccaaggcaa
     2101 caataccgcc cacaaccgcc gcactaatcg catgtaaggg ggcagcccag cgccactgct
     2161 gacgcgcacg ttccatccac ggcgcaccca aaaacaccag ggcaaaactg ggcaaaaacg
     2221 tgaaccaagt caccactccg gcgccgatca agcctgcttg ccaggtgggc atgggtaacc
     2281 cggcctgcca gcctgccaca aagccaatat aggtcacgac cataatcaag gggccaggcg
     2341 tgctttcgcc taacgccaag ccatccagca tttgcagcgc atttaaccag tgaaaatgtt
     2401 gcacactggc atcaaacaca taaggcaaca ccgcataggc gccgccaaaa gtcagaaacg
     2461 cagccttgct aaaaaacaag gctaactggc tgaacacatg cggccatccg tagcaaagta
     2521 cgagccccat caatccgcca gcaccgagca gcgccgcaat acccagcgtc caaacgatca
     2581 aaggctgtat agggccgatc actgccgggc tggtatggtc atcaatcaca caaggtaaat
     2641 gcgacgtaga ggccacgtga ctgtgtgatg tttgaggttg gactcgcatc ggccaatatt
     2701 gttgcatgag ccaacccacc accccggcca acaggatgat tgtcggaaac ggcacatcca
     2761 gcacatgcag gcataaagcc agtaaaaaca gagcccatag ccagctagca tgcaatactc
     2821 gcttggccag gcgccaggca gcgctaaata tcagcgcaac cacaagaggc ttcacaccat
     2881 acaataacgc cacgacttcg ggctgactac cccattgcat atacatccag gataatgcaa
     2941 ccaacaacgc aaatgcgggc aatataaaca aactgcctgc gacaatgcca ccccataccc
     3001 catacaataa ccagcccata tacaccgcca tttgctgcgc ttcagggcca ggcagcacca
     3061 tgcaataatt caatgcatgt aaaaagcgcg catctgaaat ccaacgcttt tctaccacca
     3121 gatcctggtg catcaaggca atttgtgcgg ccggcccacc aacgctgata cagcccagcc
     3181 gaaaccagta cctccagaag acggccgggg tcgggcgcac aggtgctgtt tgtgcttgcg
     3241 tgatattgct ggcagacatg ccaatccctc tttaataaaa tcgataccag tgctggcttg
     3301 cgaagcatag caggattttt tttatctttt gtgtgcattc ttgatgttct ttgctataat
     3361 gcgcgtcttg cgattcggga ctagcccaaa ttgctgttgg ccagatagct cagtcggtag
     3421 agcagaggat tgaaaatcct cgtgtcggca gttcgattct gcctctggcc accatttctt
     3481 accgcctctc ggcgcttctt ttttctgggt ttgttgatgt cattaaaaca cttaacaccg
     3541 gatgaattac accccttact gagcgaccca tcgttgctgc tggtcgatat ccgcaatgac
     3601 catgaactgg tttccggaat attacctgga gcactgcatg taccgttagg caacctggct
     3661 gccgtatttg accagtttcc acaagacaag aagattgttt tttattgccg aagcggtgtg
     3721 cgctcggttg ctgctggcga gtttgcgctc gcccaaggcc ggaccgatgt atcgcatctg
     3781 gcaggcggaa tactcgcctg ggcaaatgca ggcatgccca tacaggccgc ttgatttatc
     3841 catcaggaac atccatgcaa ttcgataaag aagtagatgc ccgcaacctg aactgtccac
     3901 tgcctatttt gcgctgtaaa aaagctttga atgagctggc agccgaacaa gtactgaaaa
     3961 tcacagcaac agatccagga tcaaaaaaag attttgatgc cttttgccgc caaaccggcc
     4021 acaccttgct agacatgcaa gaagcagaag gcgtatttac ctttctcatc cgcaagcgca
     4081 gccagtaact ccccgtccac agatgagcgc ttaggcacca actgcgccgc accttgctca
     4141 cttaattcca cacaaaaaaa gggcttccga agaagccctg aggaacgacg ctaacccttg
     4201 ggttaacgtg gtctgcatac cgaacaacga ttgctaagct ggcatactca gggacttaac
     4261 ctgactgaca gacttgtcgg catggcttcc gacaagccgc acactccaga cgcaatcatc
     4321 gctatcttaa gtatgtttac ggctaccgga gactaaactt tagggcgatc agcaaacatt
     4381 taaataaaaa aagcccgcca atactggcgg gcttttcgca gattgagcgc acgtggcgct
     4441 gaatttactt gacgctttcc agactgccca tcgctttagg gaatgtcacc acaccccaag
     4501 gcatgttttt cacttccact tggttagtga cttccagctt gtcggcatca atcacaaata
     4561 ccgcgtcaga tttaccacag gccaacagga cgttcttgtt gtccggggta aagctgaaat
     4621 gccagcaacg gttgccagta ggcacttcct tgattttttc aaagcccttg gcatcaaaca
     4681 cttgcagcaa cttggcttta ttggtcgcaa caaaaatacg ctcacccttg ctgtcatagg
     4741 agataccata tggcgtctca gcggtatcga cggtgcgcac tacattgaag tccttatcca
     4801 tcaccatgaa tttgttaccg aactccagcg ttgccaggta ggtattgcca tcaggagaca
     4861 ctttaatgcc gcgcggccga tcaccatact cgtgcgtagg aatggttttc accagctcac
     4921 cggtctcaat gttatgcaca gtcaccgtgt tatcggcttc attggtaatc accaactgtt
     4981 tgccatcggc cgagaactcc aaaccttctg tctccgggcc gcccgtgatt tcacggattt
     5041 tttcaccctt gcttaaatcg atcaccgcaa ttttggccgg aattttctct tcatcatcgt
     5101 catcatcgtc ctcttcaggc ttggcacctg gttttggagg cgggcctgct tttgcgctag
     5161 gctcggatga gacaaacgca taattaccac tgacgcgaac aaactcaggg ttcttaccta
     5221 cctcaatttg cttcaccact tcgcctgtgg cggtatcaat cacagaaaca ctgccatttt
     5281 cacgggtggc aaccaccagt tgcttgccat catctgtgac tgccaggcca cgcggaccct
     5341 gcgctttgac atcgaactgc ttggtgacct gcatggtcgc caggtcaatc acgttgacac
     5401 ccgcatcttg tgtagagaca tatgccactg caccagcagc actttcggtg gcaggtgccg
     5461 cagcctctgc agcaggctct tcctgcttgt tacaagcgga caccgttgca accaacgcca
     5521 gcaataagaa gctcatgcgc gtacgacgcg ctaaaggtga attcaacatt tgcatctatc
     5581 tcctgatttt ttattaaagc ttatgactcc agcaagaatc attctcacgc gcaaaccctt
     5641 tgattcgagc attccacgcg acattacgac caaaacggct gcaaacaacc accaacccgg
     5701 tccatttcag ccacctaccc caaaaacagg cggtaggccg gattctgact ttcatcccaa
     5761 caagggtaac ccagctgctg cagaaattgc gcaaatgcca ctttatcctc tggcggcacc
     5821 tgcatcccca ccagcacacg gccaaaatct gcgccatgat tgcggtagtg gaacaaactg
     5881 atgttccaat tgtgacccat ggattccaga aagttcatta atgcaccagg tttctcgggg
     5941 aactcaaaac gatacaccac ttcattttgt gcctgcggcg catgtccgcc gaccaaatgg
     6001 cgcaaatgca gtttggccat ttcgttatca ctgagattca gtgtcggcaa ccctgcttgc
     6061 tccagcttac tggcaatctc agctgcctcc gccgggttat gcacggcgat accgacaaaa
     6121 atgtgggctt ctttggcatc cgagaatcga tagttgaatt cggtgatact acggttgcct
     6181 aacaggcggc aaaacgcctt gaatgcgccc ggtgtttctg gaatggtcac cgcaaatacc
     6241 gcttcacggt gctcgcccaa ctcagcacgt tcggcaataa agcgcaggcg gtcaaagttc
     6301 atgtttgcac ccgaggccac cgcaatcatg gttttacctt gcaactggta tttttcgcta
     6361 tacgctttta acccagccaa tgccagagcg ccagcgggtt cgagaatact gcgcgtatcc
     6421 tcaaacacat ccttgatcgc agcgcagacc tcgtcattat cgacgacgat catttcgtcg
     6481 acatattgct gcaccagggc gaatgtatgc tcacccactt gcttaaccgc tgcaccatct
     6541 gcaaacaagc ccacttgttc cagcatgaca cgctgcccgg ttttcaatga ttcggtcatc
     6601 gcttcagcat cctgtgcctc tacgccgatg actttaatct cgggccgcac tgccttgata
     6661 tacgccgcaa tcccggccag caagccacca ccgccaacac agcagaaaat cgcctctatc
     6721 ggttcaggat gatcctgcac cacttccagc gcaatcgtgc cttggcctgc aatcacatcc
     6781 ggatcatcat acgggtgtac aaaggtcagg ccttcgtttt tttcaacctc cagagcgtgc
     6841 gcataagcat cggaatagct gtcgccaatt aacacgactt cggcgccacg tgaacgcacg
     6901 gcattgacct tgattgccgg tgttgttgtc ggcatcacaa tcacggcgcg gcaacccatt
     6961 ttttgcgcac tgagcgcaac gccttgtgcg tggttaccgg cactggcgca aatcacacca
     7021 cgcgccaatg cctctggcga aaggttagcc attttgttat aggcaccacg cagcttgaac
     7081 gaaaacacgg gttgcatgtc ttctcgcttg agcaatatcc ggtttttaaa gcgtgctgac
     7141 aggttaggca tttcatccag gggcgtgtgc ctggccacag catagacttg ggagtgttcg
     7201 attttttcgc ggtagttgat ggtcatgaaa tgcgtttgga ttttaaaacc gcatggttgc
     7261 agtgtatgat agagaattgt ttgtattata aagaattaat caaggattga agcagatggc
     7321 gatggataaa gcacaacaag acgcactgaa actggaagtg gcgaaagcgg ctgtgactta
     7381 tgtaaaagat ggcattatcg gtgtgggtac cggttcaacg gccaactttt ttattgatga
     7441 actggccaag gtaaaacaca aaatcactgg cgcagtcgcc agctccgaag caaccgcgca
     7501 acgcctgcgt aaccacggca tcgaagtgtt tgacctgaat agcgtcgata gcctcgatat
     7561 ttacgtagat ggcgctgacg aaattactga acacatgcat atgcttaaag gtggcggcgg
     7621 tgcgttgacc cgcgaaaaaa tcgtggctgc cgtggcaaaa tcctttatct gtatctgtga
     7681 cgaaagcaag ttcgtgccag tattgggcaa gtttccgtta ccagtagaag tattaccgat
     7741 ggcacgcagt catgtggcac gtgaactggt caagctgggt ggtcagccac aattgcgtga
     7801 cttcactacc gataacggca acctgattct ggatgtccac ggcctgacca tcagtgaccc
     7861 tatcgacacg gaagccaaaa tcaaccagat cgttggcgtg gtcaccaatg gtttgttcgc
     7921 tgcccgccca gccaacgtgt tgttgttggc aacgccaaac ggcgtcaaaa ccctgaccaa
     7981 gtaattgcaa agtgatcgcg ctaaaaatta tcatactgtt gtcatattgt gctggtaacc
     8041 tgcatcggca caataattag gaaaatttgt catgcaattt gaacatacct ctaagcaata
     8101 tgatgttgag ctggaatcgg ttcgcgccaa ggttctggaa atgggagggc ttgttgagca
     8161 gcagattgtc aatgcactgg aggcactgac cagtgccgac gtgaatctgg cgaaagatgt
     8221 gatggagcgt gacacccgtg tcaacgcgct ggaagtgcag gtcgatgaag attgcagcca
     8281 catcattgcc cgcagacagc ctgctgcccg tgatttacgc atgattatga tgatggtgaa
     8341 aaccatcacc gacctggaac gtattggtga tgaagcgacc aagattgccc gcactgcgca
     8401 gcgtatttat gagcaggacc gcatgtacaa accgcgcttc aacgagatca aatccatggt
     8461 cgcgattgtg cgtgaaatgc tgcgcacctc tctggactca tttgcgcgcc tggacatcag
     8521 ccagaccgta gaggtggcca agcaggacga gcaggttgat gaccaattcc gcgcagccat
     8581 gcgtcagctg attactttta tgctggagga tccacgcacc atttccatgt cactggaagt
     8641 gttgtttgtc gccaaggcga ttgaacgtat cggcgaccat gccaagaaca tctcggaata
     8701 tgtggtgtat atggtcaaag gtaaagacat tcgccatacc agcctggaag acatccagcg
     8761 cgagacgcag tcttaaccgc aagataataa aaaagcactc ccaggagtgc ttttttgttt
     8821 gatcaatcca atgaaacaat caagattgtg gcggcacata accactggca acatcggcac
     8881 catcgccaaa aaagtatttt tcggtttgct cagccaaata cttgcgggca ctggggtctg
     8941 ccaggctcag gcggttctca ttgaccagca tggtctgctg cttaagccag ccagcccatg
     9001 cctctttgga cacgtgcata taaattttct tgcccaactc tcctgggtaa ggtggaaaat
     9061 ccatgccctc cagctccttg cccagtttta cacattgaac cattcttgcc atgttcgatc
     9121 ctcactacac tgctaaaagc gctatcatag cgcatgttaa aatgtgatgt cgcaatttag
     9181 agataaacca tgcaaaacaa tcaatcatcc agccaccagg aaagcccgtt taaaggcaaa
     9241 actggcattc gccgtttaat caatgccttt ggctattcca tggacggctt taaagccgcg
     9301 tatcaacacg aggatgcctt ccgccaggag gtgtggctgt cactggtatt aattccactg
     9361 gcgttttacc tggaaactga agccttgcac cgcatactca tggtcggtag cgtactgctg
     9421 gtgatgatgg tggagctgtt aaattcagcg gtagaggcgg tggttgaccg tgtctcgatc
     9481 gagcgtcatg cattggcaaa gcgcgccaaa gatattggca gtgcagcggt attactcgcg
     9541 ctgattaacc tgggcgttgt ttggggattg attctgctcg gctaagccag atcgtcaggc
     9601 acatacaata aaaaaggttg gcattgccaa ccttttttat ttagctaagg tttctcttaa
     9661 ctttccagac cattaaaaat cggccctggc acccaccaga acagatcgtg cacctgccgg
     9721 tgaaatatcc ttgagaaaag acgcgtgctc gcgtatctca tcattaagca ggttatttgc
     9781 cttggcaaac aattccacat gatatttgac cggaagctta tacgcgacca atgcgcttaa
     9841 gttggtatag ccattggttt tcagctcatt ttctgccaca tcattttgct tgaacgcatg
     9901 caacacatcg acgcgcgcat tccagttacc caagcggtag tccaggccgc caccgagacg
     9961 taaaggcgag attcgcggca aatactggtt attacgcgta tccttggcat gcacgtaatc
    10021 accacgcatt tttaatgtcc actcgtcatt cagggcaaac ttgccttcga gctccaagcc
    10081 cttaaacaac gccggcactg cagcgaaatt cgcaatggcc agcccggtgc ctggatctgt
    10141 tgcacccgag ttaaacaggc caataaaatt ctggaaacgt gtggcatacg cccccaaagt
    10201 aatactgtgc ccattttctt tccacttcaa ttgcgcatcc aacccattcg agatttcttt
    10261 attcagatta gcgttaccga cttcaaactg gccggtggcc acatgctcac cattggcata
    10321 taact
//
LOCUS       DATMSK010000031         6771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_26228,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000031 DATMSK010000000
VERSION     DATMSK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26228"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1031)
                     /locus_tag="VNZ84_01080"
     CDS             complement(15..1031)
                     /locus_tag="VNZ84_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing serine
                     protease family protein"
                     /protein_id="HWU65571.1"
                     /translation="MKFFLLAVLALATATANAYNQQALMEAYFGVVMIRGYNQDGGMA
                     YGSGVVVADNAVVTNCHVMRATKQPWVSRGEDSYPITTVRADAWHDLCLVTTHNMPFK
                     PVARGNSLSLTRGQEITAIGHSNGVPAPITSVGEIQGLYPTPSGNMIRSGAKFLMGAS
                     GSGLFDMEGKLIGINTFKTAGHGGSIHFALPVEWLETLEKMPASTEFPVSGKALWEED
                     EDKKPFYMRAAVPESRQDWPALERISTLWTQQEVASTDAWFSLGISQLSLNKLEAAAI
                     AFDKAVELDQQFVEAWFRRGEVAQKLGDLQTVHTVETRLQQIDPLLVTAYQAYLGCTQ
                     DCKK"
     gene            1188..3221
                     /gene="uvrB"
                     /locus_tag="VNZ84_01085"
     CDS             1188..3221
                     /gene="uvrB"
                     /locus_tag="VNZ84_01085"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWU65572.1"
                     /translation="MIVTFPGSKYELHQPFPPAGDQPQAIEKLTQGINDGLKFQTLLG
                     VTGSGKTYTMANVIARTGKPAIVMAPNKTLAAQLYSEFREFFPNNAVEYFVSYYDYYQ
                     PEAYVPSRDLFIEKDSSINDHIEQMRLSATKALLEREDAVIVATVSAIYGIGDPGEYH
                     GMVLHIHQGKKIAQKDIVAHLIAMQYDRNDFDFGRGCFRVRGDIIDVFPSENSETAVR
                     ISLFDDEIDTIQLFDPLTGQVFNKIHNFRIFPSSHYVTAREATVRAMDTIKEELRQRV
                     SFFLSENKLVEAQRIEQRTRFDLEMLNEIGFCKGIENYSRHLTGRNPGDPPPTLIDYL
                     PEQALMIIDESHVTVPQIGGMYLGDRSRKANLVDYGWRLPSAMDNRPLKFEEFERIMR
                     QCVFVSATPANYEKEHQQQVVEMIARPTGLVDPEITVKPADTQVDDLLGEIKLRVEVG
                     ERVLATTLTKRMAEDLTDYLSEHGVKVRYLHSDIDTVERVEIIRDLRLGEFDVLVGIN
                     LLREGLDIPEVSLVAVLDADKEGFLRSERSLIQTAGRAARNLNGRVIFYANTITRSMK
                     LAMDEAERRRKIQVAFNAEHGITPKGVTKRIKDIIESVYDKDEAQRERKAAQEQANYE
                     SMSEKTLTKELKRIEKAMHDAAKNLEFEKAAQYRDQLKKLKLQAFGAEVEDLK"
     gene            3218..3484
                     /locus_tag="VNZ84_01090"
     CDS             3218..3484
                     /locus_tag="VNZ84_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HWU65573.1"
                     /translation="MMTLNLCIGSIAAICTTLAFVPQVIQSWRTRDLSGISLPMYTIF
                     TTGVLLWLIYGILIRDWPVIIANAVTAILASVVLLLKLKSVIYD"
     gene            3535..4404
                     /gene="galU"
                     /locus_tag="VNZ84_01095"
     CDS             3535..4404
                     /gene="galU"
                     /locus_tag="VNZ84_01095"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HWU65574.1"
                     /translation="MKKITKAVFPVAGLGTRFLPATKASPKEMLPVVDKPLIQYAAEE
                     AIAAGATELIFITGRNKRSIADHFDKAYELEAELEAAGKQKLLDIVHSILPKGVSCVF
                     IRQSQALGLGHAVLCAKPVVGDEPFAVLLADDLLCGDESITKQMVDVYNEHQCSILGI
                     ETVAPEETSSYGVVDAIAESDDLLEVKGMVEKPKPEEAPSNLAVVGRYVLSPRIFDCL
                     EHVKPGKGGEIQLTDGISALMQYEKVKAYRYKGKRYDCGSKLGFMQANVELALKHPEI
                     ADGFKAYLKTLNV"
     gene            4412..4957
                     /locus_tag="VNZ84_01100"
     CDS             4412..4957
                     /locus_tag="VNZ84_01100"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HWU65575.1"
                     /translation="MSSPQVQPPSFWMAHSDVVFTESEVNAAIDRMALQLQEKITAES
                     VVLLCVMRGGLYLAGQLMGRLSLPARLDYLQANRYQGTAGHEIVWSKQPELDLNGQTV
                     IVLDDILDEGVTLAAVEQYCRQAGASQVITAVLTEKDNGLQKPLKADVVGLIVPNRYV
                     FGCGMDVYGWWRNLPEIRALT"
     gene            complement(5043..6488)
                     /locus_tag="VNZ84_01105"
     CDS             complement(5043..6488)
                     /locus_tag="VNZ84_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HWU65576.1"
                     /translation="MSEHTCSPGCNHGHENEAVVKEEFHEARRSFLKDAFAMGSSTAA
                     LGALGITMSPSAFAQSAKVGTGRASHFYVPANDKTVHWGFFSKSLKPLIEVESGDYVT
                     LETLTHHANDDIERMVRGDPGAESVFYWDSKRKGVDRRGIGAMDAKVGAGGGLGVHIC
                     TGPVAIKGAEPGDILEVRIVDVVPRASANPLYKGKAFGSNAAAWWGFHYGDTVEEPKK
                     REVITIYEVDATGERNYASAVYNFRWTPQTDPFGVVHPIIDYPGVPVDHKTIHENHNV
                     LKNVRVPIRPHFGTMGVAPAEADMVNSIPPSYTGGNIDNWRIGKGATLYYPVSVAGAL
                     FSVGDPHASQGDSELCGTAIECSLTGTFQLILHKKASLPGTALESLKHPLLETQDEWL
                     LHGFSYPNYLADLGPDAQNSIFTKSSVDLALRDAYRKMRDFLMGTQGLSEDEAISLMS
                     IAVDFGITQVVDGNWGVHAVVKKSIFPPRKT"
BASE COUNT         1622 a         1613 c         1808 g         1728 t
ORIGIN      
        1 gtttttatct cgccttattt tttgcagtct tgtgtgcagc cgagatatgc ctggtaagcc
       61 gtcaccagta aagggtcaat ttgttgcagc cgggtttcaa ctgtgtgcac ggtttgcagg
      121 tcgcctagct tttgggcaac ctcgccgcgc ctgaaccagg cttcaacgaa ttgttgatcc
      181 agttcaacag ctttatcgaa agcaatggcg gctgcctcca acttgttgag ggacagttgt
      241 gatatgccca ggctaaacca tgcatctgta ctggccactt cttgctgcgt ccataaggtg
      301 ctgattcttt ccagcgccgg ccagtcttgg cgagactccg gcaccgctgc acgcatataa
      361 aaaggttttt tgtcttcgtc ttcttcccac agggctttac cactgaccgg aaactcggtg
      421 ctggcaggca tcttctccag ggtttccagc cattcaaccg gcagtgcaaa gtgtatgcta
      481 ccgccgtgac ctgccgtttt aaaggtgttg atgccgatga gttttccctc catgtcgaat
      541 aggccgctgc cgctggcgcc catgaggaat tttgcaccgc tgcgtatcat attgccggag
      601 ggcgttggat aaagtccctg gatttcgccg acagaggtga tgggggctgg aacgccattg
      661 gaatgcccaa tggcagtgat ttcttgcccg cgtgtgagcg acaggctgtt gccgcgtgct
      721 acgggcttga atggcatatt gtgcgtggtg accaggcaga ggtcgtgcca ggcatctgca
      781 cggacggtgg tgatggggta gctatcttcg ccgcgtgaga cccagggttg cttggtggca
      841 cgcataacgt ggcagttggt gacaacggca ttgtctgcga cgacgacgcc agaaccataa
      901 gccatgccac catcctggtt gtagccacgt atcatcacga caccaaaata agcctccatg
      961 agggcttgct ggttgtaagc atttgcggtt gcagtggcga gtgccagcac tgccaataag
     1021 aaaaatttca cttcatcccc catgcctgat ttgtgtttgt tcaactgact ggttcttgtg
     1081 gtaaaagttc atgcgcaaat aaagcatcaa gaatgtaaat gatacgcccg tgacggcaaa
     1141 aacagttaaa atagaattta taccattctt gtttccagat tgtttttgtg atcgtcactt
     1201 tccctggcag taaatacgaa ttgcaccagc ctttccctcc cgctggcgac cagccacaag
     1261 cgattgagaa gttgacgcaa ggtatcaacg atggcctgaa gttccaaacc ctgttggggg
     1321 tgaccggctc cggtaagacc tacaccatgg ccaatgtgat tgcacgtacg ggtaaaccgg
     1381 cgattgtcat ggcccccaat aaaacactgg ctgcgcagct gtatagtgaa ttcagggagt
     1441 tttttcccaa taatgcggtc gagtactttg tgagttacta cgactactat cagccagaag
     1501 cctatgtacc ttcgcgcgac ctgtttatcg aaaaagattc cagcatcaat gatcacattg
     1561 agcaaatgcg tttgtcagcc actaaggcct tactggagcg cgaggatgcg gtgattgtgg
     1621 cgaccgtgtc ggcaatctat ggtattggtg atccgggtga ataccacggc atggtgctgc
     1681 atatccatca gggtaaaaaa attgcgcaaa aagacatcgt ggcgcacctg attgccatgc
     1741 aatatgatcg taacgatttt gattttggcc gtggatgttt ccgtgtccgt ggcgatatta
     1801 ttgatgtgtt cccctcagaa aacagtgaaa ccgccgtacg tatcagcttg tttgatgatg
     1861 aaatcgatac catccagttg tttgacccgc tcacggggca ggtgtttaac aagattcata
     1921 acttccgtat tttcccttcc agccattatg tgacagcgcg cgaagccact gtgcgtgcga
     1981 tggataccat taaagaggaa ctgcgccagc gcgtgagttt tttcctgagt gaaaacaaac
     2041 tggtggaagc tcagcgtatt gaacagcgta cgcgctttga cctggaaatg ctgaatgaaa
     2101 tcggtttttg caaaggcatt gaaaactata gccgtcatct gaccggacgc aatcctggtg
     2161 atccgccgcc gaccctgatt gactatttgc ccgagcaggc cttgatgatt attgacgaaa
     2221 gccatgtcac cgtgccccag attggcggca tgtacctggg ggaccgctcg cgtaaagcca
     2281 acctggtgga ttacggctgg cgcctgccat cagccatgga taaccgaccg ctcaagtttg
     2341 aagagtttga gcgcattatg cgccaatgcg tatttgtgtc tgctaccccg gctaattacg
     2401 agaaggaaca tcagcagcaa gtggtggaaa tgatcgcgcg tccgaccggg ctagtagatc
     2461 ctgaaatcac cgtcaaacct gctgatacgc aagtggatga tttgcttggt gaaatcaagt
     2521 tgcgtgtaga ggttggcgag cgtgtgctgg caacaacgct gaccaaacgg atggcagagg
     2581 acctgacaga ttacctgagc gaacacggcg tgaaggtgcg ctacttgcac agtgacattg
     2641 atacggtaga gcgggtcgag attatccgtg atttacgcct tggcgagttt gatgtgctgg
     2701 tgggaattaa tttgctgcgc gaaggtctgg atattccaga agtttcgctg gtggccgtcc
     2761 tggatgcgga caaggaaggc tttctgcgct ccgagcgctc cttgatccag acagctggcc
     2821 gggccgcacg taacctcaac ggtcgggtga ttttttacgc caataccatc acacgcagta
     2881 tgaagctggc gatggacgaa gcggagcgca gacgtaaaat ccaggtggca tttaatgccg
     2941 aacatggcat tacgcctaaa ggggtgacta aacgcatcaa ggatattatt gagagtgttt
     3001 atgataaaga cgaggcccag cgcgagcgta aagccgcgca ggagcaggcg aattatgaaa
     3061 gcatgagcga gaaaacgctg accaaggagc tgaagcggat tgagaaagcc atgcatgacg
     3121 ccgccaagaa ccttgagttc gagaaagctg cccaataccg tgatcagttg aagaaactta
     3181 agttacaagc ctttggtgca gaagtagaag acttgaaatg atgacgttaa atctttgtat
     3241 tggctcgatt gcagccatat gtactacgct agcatttgtg ccacaagtga ttcaatcgtg
     3301 gcgtacacgg gatttaagcg gcatttcatt acccatgtat acgattttta ccactggcgt
     3361 attgctatgg ctgatctacg gcatattgat ccgggattgg ccagtgatta ttgccaatgc
     3421 agtgacggcc atactggcga gtgtggttct tttgttaaaa ttgaaatctg tgatctatga
     3481 ctgatgtcag atttcaaaaa atatttttat gactactttc attaggatac gaccatgaaa
     3541 aaaattacaa aagcagtgtt cccggttgcc ggtctcggca cccgtttctt gccggcaact
     3601 aaagccagcc cgaaggaaat gctgcctgtc gttgataaac cgctgattca atatgcggct
     3661 gaagaagcga ttgctgcggg cgcgaccgag ctgatcttta ttaccggtcg taacaagcgc
     3721 tcgattgcag accattttga caaagcttat gagctggaag ctgagctcga agccgcaggc
     3781 aagcaaaagt tgctggatat tgtgcatagc attttgccta agggcgtgag ctgcgtattt
     3841 atccgccagt cacaggcgct gggcttgggc cacgcagttt tgtgtgccaa acctgtggtg
     3901 ggtgacgagc catttgccgt gttgctggcc gatgacctgt tgtgcggcga tgaatccatc
     3961 accaagcaga tggtggatgt gtataacgaa caccagtgct ctatcctggg gattgaaacg
     4021 gtagcacctg aagaaaccag tagttacggt gtggtggatg cgattgccga aagtgacgac
     4081 ctgcttgaag ttaaaggcat ggtggaaaaa ccaaaaccgg aagaagcgcc ttctaacctg
     4141 gccgtcgttg gccgttatgt gctgagccca cgtatttttg attgcctgga gcacgttaag
     4201 ccgggtaagg gcggggaaat ccagctgacg gatggtattt ctgccctgat gcaatatgaa
     4261 aaagtaaaag cttatcgtta caagggtaaa cggtatgatt gcggtagcaa actgggcttt
     4321 atgcaagcca acgtggagct ggcgttaaaa catccggaaa tcgcagacgg ctttaaagca
     4381 taccttaaaa cgctgaacgt ctaatcttca tatgtcctcg ccacaggtac agccgccgtc
     4441 gttctggatg gcgcattccg atgttgtgtt tacagaatcg gaagtcaatg ctgccatcga
     4501 tcgcatggca ctccaattgc aggagaaaat cacggcagaa tctgtcgtgt tgttgtgtgt
     4561 gatgcgaggt ggcctgtatc tggcagggca gttgatgggc cgtttgtccc tgccggcacg
     4621 gcttgattat ttacaagcca atcgctatca gggcactgcc ggccatgaaa tcgtctggag
     4681 caagcagcct gagctggatt taaacgggca aacggtcatt gtgctggatg atatcctgga
     4741 tgaaggcgtg acattggctg cggtcgagca atattgccga caagccggtg ctagtcaggt
     4801 gatcacagct gtgcttacag agaaggataa tggtttgcaa aagccgttaa aagcggatgt
     4861 ggtcggactg attgtaccta accgctatgt attcggttgt gggatggatg tatatggctg
     4921 gtggcggaat ttgccagaaa ttcgtgcctt aacctaatac cattctagcg acttatttca
     4981 tcaccttaag gctgctacgg cactgttact acataccgta gcagccctgt aggcatgtaa
     5041 atttaagtct tgcgcggcgg aaagatactt ttcttcacga cggcgtgtac gccccagtta
     5101 ccatcaacca cctgggtaat gccaaagtcc accgcaattg acatgagtga aatggcttcg
     5161 tcttcactta atccttgtgt ccccattaaa aagtcgcgca ttttccggta agcgtcacgc
     5221 aaggctaaat ctactgagga tttggtaaaa atactgtttt gagcatcagg tcccaggtca
     5281 gccaggtaat ttgggtagct gaagccgtgc agtaaccatt cgtcttgtgt ttccagcaac
     5341 ggatgtttca ggctttccag tgcagtgcct gggaggctgg cctttttgtg caaaatgagc
     5401 tggaaggtcc ctgtgaggga gcactcaatc gctgtcccgc ataattcaga gtcgccttgt
     5461 gacgcatgcg ggtcgcccac cgaaaataat gccccggcaa ccgacacagg gtagtacagg
     5521 gtggcgcctt tgccgatgcg ccagttatca atgttgcctc cggtatagct tggtggaatg
     5581 gagttgacca tatccgcttc agcaggcgca acccccatgg tgccaaagtg cgggcggata
     5641 ggcacacgta catttttaag gacgttgtga ttttcgtgga tggttttgtg gtccacgggt
     5701 accccagggt aatcaatgat ggggtgcacc acgccaaacg ggtctgtttg tggcgtccag
     5761 cggaagttat acaccgcgct ggcatagttt cgttcacctg tggcatccac ctcgtagata
     5821 gtgatgactt cacgtttttt tggttcttcc accgtgtcgc cataatgaaa tccccaccag
     5881 gcggctgcat tactgccaaa agccttgcct ttgtagaggg gattggcgct cgccctgggc
     5941 acgacatcaa caatacgtac ttctaatata tccccaggtt ccgcgccttt aatggccact
     6001 ggcccggtgc aaatatgcac gcccagcccg ccacctgcgc ctactttggc atccatggct
     6061 ccgatgccac gacggtctac ccctttacgc ttactgtccc agtaaaatac actttctgca
     6121 ccggggtccc cgcgtaccat gcgttcaata tcgtcattgg cgtggtgggt aagcgtttca
     6181 agtgttacat aatcccctga ctctacttca atcaatggct tgagtgattt actgaaaaat
     6241 ccccaatgca ctgttttatc gtttgcgggg acatagaagt ggctggcgcg cccggtaccg
     6301 actttggcgc tttgggcaaa tgcgctcggt gacatggtga tgcccaaggc acccagggct
     6361 gccgtactac tccccatggc aaatgcatct ttgagaaaag atcttcttgc ctcgtgaaac
     6421 tcttctttga ccactgcctc attttcatgg ccatgattgc aaccgggact acaagtatgt
     6481 tcagacatat tgaaaaactc cactttgttt aagaatgcgc aaatgagttt ttattcaaga
     6541 ccgcggtcga tctcgaatgg ttgatcacga ttgaatgaca aggcttgcca ggcattaatg
     6601 aactaatatg ttcttgaatg tccctgtatt ctgatctgtc tcaagctggt gcaagggcgc
     6661 tcagtgcatg cgatctaaat ccatcatcaa tgatatgccg taagttagtt gtggcttgca
     6721 tgttgataaa accactgcat gcatgcgctg ccaagcgtgt tcgatggcac t
//
LOCUS       DATMSK010000032         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_237694,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000032 DATMSK010000000
VERSION     DATMSK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237694"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="VNZ84_01110"
     CDS             complement(<1..108)
                     /locus_tag="VNZ84_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HWU65577.1"
                     /translation="MNKLLIALLLSCVCVSAYAGPQQEKMKTCNADAKTK"
     gene            complement(245..2179)
                     /locus_tag="VNZ84_01115"
     CDS             complement(245..2179)
                     /locus_tag="VNZ84_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWU65578.1"
                     /translation="MKNQLINCLIGSIFATLASSAWADINDVRANLSKLHVPEGFHVD
                     VYAEVPGARQMALGTNGNVYVGTRGNKVYAVVDKNKDHKADQVVTILDDLNVGNGVAM
                     VDGHLYVAEQHRITRYAAPDFDLTLPFKAMREVVYDKLPNKAHHGWRYLTTGPDNKLY
                     VTVGAPCNICDPAGIEASIIRMNVDGSQVETFAKGVRNSVGMDFQPGTNTLFFTDNGV
                     DLLGADVPHDELNAAPKAGLHFGFPYFAGGDARDPNWKDKTPPAVTKPVAEFQAHSAN
                     LGFKFYTGKQFPAEYQGNAVIAQHGSWNRKVPVGYQLVRVTFDDQHQVKETKPFIDGW
                     MSAEGEVWGRPNDVLQLPDGSLLVSDDYNGVIYRVSYDGKTQSTATTAAEANTLSGFV
                     MPESVFATPEGVTYLSEIGEFGKLGDGKITRIAADGTRTTLADGLNDPKGLDVFDGQL
                     YVADMDRVVRIDTHSGTQTVLAAATAFPQKPVFLNDIEIDGLGNIYVSDSGDDHGNGA
                     GIFKISPAGKVTQVLRAKAGIQRPNGLLMDGPDRLLVADFGTGKLFSVQIGSKKTTVS
                     LLNQGFGGADGLIRDAHGLLYVSDWAGGNVWQLVEPKATPQRIIQGYQSAADISLSAD
                     GKSLLIPDMKAGTLHSFPIH"
     gene            2368..3336
                     /locus_tag="VNZ84_01120"
     CDS             2368..3336
                     /locus_tag="VNZ84_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWU65579.1"
                     /translation="MSSGECWHAHDRDRTITLLSALTPTEGFTLTALDGVKLMRWNRA
                     IPRSPVFYEPSIVFVCQGRKIGYLGGEVYQYNPQQFLILSVPLPFESETIASAEEPLL
                     AISIRINLSMVSELLLSLTHDAPVNINGKSGISSTPLDMRLSNAVVRLLECLQSRTEA
                     AVLGQSIIREIHYRILQSPQASAILAAHSTHNNMGKISKALRLIHAEYANELNVDKLA
                     AESAMSVPSFHAAFRDVTATSPIQYLKKTRLHKARLLMVQDGINATLAATKVGYESAS
                     QFSREFKRMFGKSPMEEVTAVRSALITSPGEFVANQRVDQYVTVQQ"
     gene            3519..>4395
                     /locus_tag="VNZ84_01125"
     CDS             3519..>4395
                     /locus_tag="VNZ84_01125"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWU65580.1"
                     /translation="MKTAAYGVQNATSPVAPMSIDRREAGPHDVLIDIEYCGICHSDI
                     HQARNEWGGGSLYPMVPGHEIVGRVKSIGSHVTKFKLGQLVGVGCMVDSCRHCEECHA
                     GEEQYCDSSVFTYNAKDPKHGGLTFGGYAERITVDENFVVSVPENLDTKAVAPLLCAG
                     ITLYSPLKHWNVQPGQKVGIIGLGGLGHMGVKLAAAMGAHVVMITTSASKAADATRLG
                     AHEVLISKDADDMAKHAASFDLIIDTVPVAHDLNPYIGLLKRDKTIVLVGPLDPVEVH
                     GANLITKRRHVAGSLI"
BASE COUNT         1058 a         1090 c         1139 g         1108 t
ORIGIN      
        1 tttagttttc gcgtctgcat tacaggtttt cattttttcc tgttgcgggc cggcgtaggc
       61 agatacacag acacaggaca gcaacaatgc aatcagcaat ttattcatga aagtctccaa
      121 acagaaagta aacgttattc gaatttgagg ccgcctgcag gcgaacccgt actctcattt
      181 tgacacagag cgaaatgctt ggggaataaa aaagcccagt gttttaccgg gctttttgag
      241 cagcttaatg aatcgggaag ctgtgtaatg tgcctgcctt catatcagga atcaggagcg
      301 atttaccatc ggcggacaga ctaatgtcgg ccgcagattg atagccctgg ataatgcgtt
      361 gcggcgtggc tttaggttct accagttgcc agacattacc acctgcccaa tcactgacat
      421 acaacaagcc atgtgcatcg cggatcaggc catcagcacc accgaaaccc tgattgagca
      481 agctcacagt ggttttcttg cttccaatct ggacactgaa cagtttgcct gttccaaagt
      541 cggccaccag taagcggtca gggccgtcca tgagcaagcc attcgggcgt tggatgccgg
      601 ctttggctct cagcacttgc gtgactttgc ctgctgggga aattttgaag atgccagcgc
      661 cattaccgtg gtcatcaccg ctgtcagata cataaatgtt gccaaggcca tcgatctcga
      721 tgtcattcag gaacacaggc ttttgtggga acgcagtggc tgctgccaat acggtttgtg
      781 taccactatg cgtatcaatg cggacgacac ggtccatgtc ggccacatac aattgaccat
      841 caaacacatc caggcctttt gggtcgttta agccatcggc cagagtcgtg cgcgtgccat
      901 ctgcagcaat gcgtgtaatt ttgccatcgc ccagtttgcc aaattcaccg atctcagaga
      961 gatatgtcac gccttcaggc gtggcaaaaa cagattccgg catcacaaaa ccgcttaagg
     1021 tattagcttc ggcagctgtg gttgcagtgc tctgagtttt accatcgtag ctgacgcgat
     1081 aaatgacgcc gttgtagtca tccgatacga gcaaagagcc atctggtaat tgcaacacat
     1141 cgttagggcg gccccagact tcaccttcag cactcatcca gccatcaata aatggtttgg
     1201 tttctttcac ttgatgctgg tcgtcgaatg tcacgcgcac cagttggtag ccaacaggga
     1261 ctttacggtt ccaggagcca tgttgtgcaa tcacggcatt gccttgatac tcggccggaa
     1321 actgtttgcc agtgtaaaac ttgaagccga ggttggcgct atgcgcctga aactctgcca
     1381 caggtttggt cactgcaggt ggtgttttgt ctttccagtt tgggtcgcgt gcatcgcccc
     1441 cagcaaaata gggaaagcca aagtgcaacc cggccttagg tgcggcgttg agttcgtcat
     1501 gcggcacatc agcacccaac aggtctacgc cattgtcggt gaagaataag gtgttggtgc
     1561 ctggctggaa gtccatgccg acggagttac gaacgccttt ggcaaaggtc tcaacctgac
     1621 tgccgtcgac attcatgcgg ataatagagg cttcaatgcc tgcggggtcg caaatgttgc
     1681 atggtgcacc gacggtcacg tacagtttgt tgtccgggcc ggtggtcaga tagcgccagc
     1741 catgatgagc cttgttgggt aatttgtcat agaccacctc acgcatggct ttaaaaggca
     1801 gggtcaagtc aaagtcgggt gctgcatagc gtgtaatgcg atgctgttca gccacataca
     1861 gatggccatc gaccatggca acgccgttac ccacattcag gtcgtctaaa atagtgacca
     1921 cctgatctgc tttgtgatcc ttgtttttat ccaccacggc atatactttg ttgccacgtg
     1981 tgccgacgta gacattgcca ttagtgccca atgccatttg gcgagcgccg gggacctctg
     2041 catacacgtc tacatgaaac ccttccggta catgtagttt gcttaagttg gcacgcacat
     2101 cgttaatatc tgcccaggct gagctggcca gcgttgcaaa tatggaaccg atcaggcaat
     2161 tgataagttg attcttcatg acgactatcc ctttctccaa gaatattgca atcaatctaa
     2221 ttgcgatatg cggtacagtg taagcagtca aggtgcggct ggaaagttaa aaaatatgaa
     2281 atgattgcct aatcctattt gcatattggt gcttaaagtc atttagtcgt aatatgaatg
     2341 cctaattatt ttgtttgtgg acatattatg agtagtggtg aatgttggca tgcccatgac
     2401 cgtgatcgca caattacctt gctgtcagca ctgacgccaa cagaaggctt taccctgact
     2461 gcgctggatg gcgtcaagct gatgcgttgg aatcgcgcga ttccgcgtag tcctgtgttt
     2521 tatgagccca gtattgtctt tgtatgccag ggtcgcaaaa tcggctacct ggggggcgaa
     2581 gtctaccaat acaatccgca gcagttcctg attctttcgg tgccactgcc gtttgaaagc
     2641 gagacaatcg cctcggcaga agagcctttg ctggcgattt ccatccgcat caacctatcg
     2701 atggtgtctg agttactttt aagtctgacg catgatgccc ccgtcaatat caatggcaaa
     2761 agcggtatta gctcgacccc gctggatatg cgcttgtcga atgcggtggt caggttgctg
     2821 gagtgcctgc aatccagaac cgaggcagcc gtattggggc aatcaattat ccgtgagatt
     2881 cattaccgta tcctgcaatc gccacaagca tctgccattc tggcggccca tagtacccac
     2941 aacaatatgg gcaaaatcag caaggcactc aggttgattc atgccgaata tgccaatgag
     3001 ctcaatgtgg acaaactggc agcagagtct gccatgagtg tgccttcttt tcatgcagcg
     3061 ttcagggatg tgacggctac ttcgccgatt cagtatttga aaaaaacacg cttgcataag
     3121 gctcgcctgc tcatggtgca agacgggatt aatgcaacct tggccgcgac caaagttggc
     3181 tacgagagcg cctcacagtt cagtcgcgag ttcaagcgca tgtttggcaa aagcccgatg
     3241 gaggaggtga ctgctgtacg cagcgcattg atcacatcgc ccggcgaatt tgtggctaat
     3301 cagagggttg accagtatgt gactgttcag cagtaatacg gacattatga caatcaaatc
     3361 acgatttctt tgtgtcaata cgcctatatc aaagaggatt aggcaagcat ttgataaaag
     3421 caggcaagcg tttttgagag accttgttta gactatttgg caatacagtg atgttggtgc
     3481 cactggttaa tatctaatta atagatgatg gagatgctat gaaaacagcc gcctatggag
     3541 tacaaaatgc gacctcaccg gttgcgccca tgagtattga tcgccgcgaa gcagggccgc
     3601 acgacgtcct gattgatatc gagtactgtg gcatctgcca ctcggatatt caccaggcac
     3661 gtaacgaatg gggtggtggt tcgctgtatc ccatggtgcc tggccacgaa attgtcggcc
     3721 gcgtgaaaag tatcggcagt catgtcacca agttcaagct ggggcaattg gttggagtcg
     3781 gctgtatggt cgactcctgc cgccactgcg aagagtgtca tgctggtgaa gagcaatatt
     3841 gcgatagctc ggtatttacc tacaacgcca aagaccccaa acatggtggc ctgacctttg
     3901 gtggctatgc cgagcgtatt accgtcgatg aaaattttgt ggtcagcgtg ccggaaaacc
     3961 tggataccaa ggccgtagcg ccattattgt gtgcaggcat caccttgtac tcgccgctca
     4021 agcactggaa tgtacaaccc ggccaaaaag tcggcattat cggtctcggt ggcttgggcc
     4081 atatgggcgt caaactggcc gccgccatgg gtgcgcatgt ggtgatgatt accacctccg
     4141 ccagtaaagc ggctgatgcc acccgtttgg gtgcgcatga agtgttgatc tctaaagatg
     4201 ctgatgacat ggccaagcat gccgccagtt ttgacctgat tattgatacc gtgccagtcg
     4261 cacatgacct gaatccttac atcggcctgc tcaaacgcga taaaaccatc gtgctggtcg
     4321 gcccgctgga tccggtcgaa gtgcatggcg ccaacctgat taccaagcgt cgccatgtgg
     4381 ccggttctct gattg
//
LOCUS       DATMSK010000033         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_190555,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000033 DATMSK010000000
VERSION     DATMSK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190555"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2466)
                     /locus_tag="VNZ84_01130"
     CDS             complement(<1..2466)
                     /locus_tag="VNZ84_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWU65581.1"
                     /translation="MYPSDQPDEIDPRKTHAFDAVLDAEIRGMSQTRQEVNRNNLIGL
                     AFSGGGIRSATLNLGILQGLAKKGLLPKFDYLSTVSGGGYIGAWFSSLVKHNSHVNTV
                     QAELVLSTQPQGEAGRQPTPTEKAILWLRSYSNYLTPKTGLFTGDTLSALAQWMANTL
                     LNQVLLLCVLICLMMTVIFLSHSDIHPALVSTFTLNIDLFSGLLNNAWQTTMGQTGIG
                     LAIASLLASFYFSRQACLSADHARYPLIAFICGVAGFTLLLWACPLQSWQDILSYPAG
                     NHESHIRPNYWIGLGVPVVSLLISAGLVAIMGLGGRWIDVSTREWWHRIGGINLGFCV
                     IWLALFGCALYIPYLIHQFWGNPDNTTKSLAGVGSWLLAFLTSKLGKSQATGPESPNS
                     LKGKLTYLLPYIVVALILLVSGYLSFSLYISPHRSTWFFGALLVVVVGSWRIDINVFS
                     LHNFYRNRLTRCYLGATNNARKPNTFTGFDNQDDLPMSALRGQRPTHIVNTAVNISDG
                     SQLAWQQRQAASFMFSPYYCGFKLPDNEVWYDTYHYTGKGGPELGSLVSTSGAAASPN
                     MGYHTNPVMGFIMTIFNARLGRWFGNPLHENYLNRLLAHWRCQYRLRNPAERQSPFWN
                     LFYLIKELITNTGSTSGYLYLSDGGHFENLGIYELVRRRCKLIVAVDAGADGNYEFED
                     LGNAIRKCKVDLGITIKLDISALLPVNDCKYGLFKCGTRHFAIGTIDYLGDGDPEHMG
                     YLVYIKSSITGDEPSDLINFKLQEPCFPHHSTVDQFFNESQFESYRRLGEHMVQNLKL
                     PTGSPEHAALLQIQQLMPGLIALQ"
     gene            2665..3513
                     /locus_tag="VNZ84_01135"
     CDS             2665..3513
                     /locus_tag="VNZ84_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HWU65582.1"
                     /translation="MRWIWLVTTVFLLLSQQVRATEKGLFWQLESPDGQVSYLFGTMH
                     TDDNRVTDFSPSVIQALKNVDTFMLEVADAPPASLLQLPQGNLKQYLSETELEQVARL
                     AEVHVMPMDMALKMKPWLLAVLFDLPKPQTPFAQDLVLKAKAEELDKKVVGLESPQEH
                     FGVMDSLTTDEQLQMLRAVLKRSQKQKESDFEHLMKAYLAGDAEQVANMDDAMTRGVI
                     PAALWQRMRVKLLDERNLLMAERSMDQAKKGKVFIAVGAAHLAGKNGLLQAFRQAGFK
                     LTPLKK"
     gene            complement(3546..4025)
                     /locus_tag="VNZ84_01140"
     CDS             complement(3546..4025)
                     /locus_tag="VNZ84_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65583.1"
                     /translation="MMSITTWELLSYVVTVIGLPLAILTFVLEQRKERENEDEEVYQL
                     LADNYTDFLKLVMANPDLQLRSHHGPLPMTAEQEERKLVLFEILISLFERAYLLAYDP
                     DMRGKRLRRWMSWEDYMREWCQREDFRQQLPRLLVGEDADFVAHITRIAYEVTQQRT"
     gene            complement(4063..>4338)
                     /locus_tag="VNZ84_01145"
     CDS             complement(4063..>4338)
                     /locus_tag="VNZ84_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWU65584.1"
                     /translation="SDEAIDLLNKRQYDMIVLDIELAKGTGFDVVRHTRQQNYPFKTP
                     DIVMLTNHNNSYYRDLAQNMGVEYFFDKSLQFDECIDVIQQHASELT"
BASE COUNT         1123 a         1012 c         1155 g         1048 t
ORIGIN      
        1 ttgtaaggcg atcagccctg gcatcagttg ctggatttgt agtagagcgg catgctctgg
       61 tgagcctgtg ggtaatttaa ggttttgtac catatgctcg cccagccggc gatagctttc
      121 aaactgggat tcattgaaga actggtcaac agtgctgtga tgcggaaagc aaggctcctg
      181 caatttgaaa ttgatcaggt cgctgggctc atccccggtt atagagcttt tgatatagac
      241 cagatagccc atatgctcag ggtcgccatc gccgaggtaa tctatggtgc caatcgcaaa
      301 gtggcgggtg ccacatttga acagtccata tttgcagtcg ttgacaggca gcaaggcgct
      361 gatatccaac ttgatggtga tgcctaaatc aactttgcat ttacggatgg cattgcccag
      421 gtcttcaaat tcataattgc catcggcacc agcatcgaca gccacgatca gcttgcaccg
      481 gcgccgcacc agttcataaa tgcccaggtt ctcaaaatgg ccgccatctg ataaatacag
      541 gtagcctgag gttgatccgg tattcgtgat gagctccttg atcaggtaaa acaggttcca
      601 gaatggcgat tgacgttctg ctggattacg cagacggtat tgacaacgcc agtgtgccag
      661 tagccggtta aggtagtttt catgcagggg gttgccaaac cagcgaccca gcctggcgtt
      721 aaaaatagtc atgataaaac ccatgaccgg attggtgtgg taacccatat tggggctggc
      781 ggcggcgccg ctggtcgaga ccaggctgcc taattccgga cctcccttgc ctgtgtagtg
      841 ataggtgtca taccagactt cattgtctgg caatttaaaa ccgcaataat aagggctaaa
      901 catgaacgag gcggcttggc gttgttgcca ggccagctgt gagccgtcac tgatattcac
      961 cgcagtgttc acgatgtggg tcgggcgttg tccacgcaag gcggacatgg gcaggtcatc
     1021 ctggttgtca aagccggtaa aggtgtttgg tttcctggca ttattagtgg cgcccagata
     1081 acagcgtgtg aggcggttac ggtaaaagtt atgcaatgaa aacacattga tatcgatacg
     1141 ccagctacct accacgacaa ccagcaaagc accgaaaaac cacgtgctcc ggtgaggtga
     1201 aatatacaag ctgaaactca ggtatcccga gaccagcaat atcagggcga ccacaatata
     1261 aggcaacagg taagtgagct tgcctttgag gctattgggg gattcaggac ccgttgcctg
     1321 gcttttaccc agcttggagg tcaaaaaggc cagtaaccac gagccgacac cggccaggct
     1381 ttttgtcgta ttgtccggat taccccagaa ttgatggatc aggtagggaa tgtaaagcgc
     1441 acacccaaag agcgccagcc agatcacgca gaagccaaga ttaattcctc cgatacgatg
     1501 ccaccattcg cgtgtactga catcaatcca tcgtccacct agtcccatga tcgccaccag
     1561 gccggcggaa atgagtaatg aaacgaccgg gacacccaaa cctatccaat agttgggcct
     1621 gatgtggctt tcgtgatttc cggcaggata gcttaggatg tcttgccagc tttgaagcgg
     1681 acatgcccat aacagcaagg taaagcctgc gacaccgcat ataaacgcaa tcaatggata
     1741 acgtgcatgg tcggcactca ggcacgcctg gcgactaaag tagaaagagg caagcagact
     1801 cgcaatcgcc aggccaatgc ctgtttgtcc catggttgtt tgccaggcgt tgttaagcaa
     1861 gcctgaaaac aggtcgatgt tgagcgtgaa tgtggatact aaggctggat gaatgtccga
     1921 gtggctcagg aaaatcactg tcatcatcag gcagatcaag acgcagagaa gcagcacttg
     1981 attgagcaaa gtattggcca tccattgtgc cagtgcggat aaggtatctc ccgtgaataa
     2041 accggttttc ggtgtcaggt agttgctata gctccgtagc cataggatag ccttttcagt
     2101 gggtgtcggt tgtcggcctg cttccccttg aggttgtgtg gataaaacca gttcagcttg
     2161 aacggtattc acatggctgt tgtgcttgac caggctggaa aaccaggcgc cgatgtaccc
     2221 gcctccggag acagtggaaa gatagtcaaa cttgggcaac agtccttttt ttgcaagtcc
     2281 ctgcaatatt cccagattta gtgtggcact ccggatacct cctcctgaaa atgccaatcc
     2341 aatcaaattg ttgcgattga cctcttggcg ggtctgcgac atgccacgaa tctcggcatc
     2401 cagtaccgca tcaaaggcat gcgttttacg cggatctatt tcatcaggct ggtcggaagg
     2461 atacatggca ctctcccatc atttatcgtt gatattattt aacacatggt atatccgtat
     2521 caaatatgtg acaacactct gttgcgacgg tcaatctcag tgacaggcaa atcatggcac
     2581 agcctcatga tttgtgagag gatgtggggc aggcatgctc acagggcagt gttgtcagct
     2641 tgagaaagaa aaatggggaa tacgatgcgg tggatatggt tagtgacgac agttttttta
     2701 ttactgagcc agcaggtgag ggccactgaa aagggattgt tttggcaatt agagtccccg
     2761 gatgggcagg tcagctacct gtttggcacc atgcatacgg atgataaccg ggtcactgat
     2821 ttttcaccgt cagttataca ggcattgaaa aatgtagata catttatgct agaggtggca
     2881 gatgcgccgc cagcaagttt attgcaattg ccgcagggta atttaaagca gtatttgtct
     2941 gaaacagagc tggaacaagt ggccaggcta gctgaggtcc atgtgatgcc aatggacatg
     3001 gcgcttaaga tgaagccctg gttgcttgcg gtattgtttg atttgcccaa accacaaact
     3061 cctttcgctc aagacctcgt gctgaaagcc aaagcggagg agctggataa aaaggtggtg
     3121 gggctagaga gtccacagga gcatttcggc gtcatggata gcctcacgac ggatgagcaa
     3181 ttgcagatgt tgcgtgcagt gctgaagcgt agccagaaac aaaaggaaag cgattttgaa
     3241 cacttgatga aggcttacct tgctggcgat gccgagcagg tggccaatat ggacgatgcc
     3301 atgacccgtg gcgtcatccc ggcggcatta tggcagcgta tgcgtgtcaa gctcctggat
     3361 gagcgcaatc tgttgatggc cgagcgcagc atggatcaag cgaaaaaggg taaagtgttt
     3421 attgcagtag gggccgcaca tctggctgga aaaaatggct tgctacaagc tttcaggcaa
     3481 gcaggcttta aactcacccc gcttaaaaaa taacagctaa agataacggc caaaaataac
     3541 tacgttcaag tccgctgttg agtgacttcg taggcaatgc gcgtaatgtg tgccacaaaa
     3601 tcggcatctt cgcctaccag taatctcggt aactgctggc gaaaatcttc acgctgacac
     3661 cactcgcgca tgtaatcttc ccatgacatc cagcgtcgta ggcgtttgcc acgcatatcg
     3721 gggtcatagg ccaaaagata ggcgcgttca aacagggaga tgagaatttc aaacaagacc
     3781 agcttgcgtt cttcctgttc ggcggtcatg ggtaacgggc cgtgatgaga ccgcaattgc
     3841 aaatcgggat tggccatcac caactttaga aagtcagtgt agttatctgc cagtagttgg
     3901 tagacttctt catcttcgtt ttcacgttct ttgcgctgct caagtacgaa agtcaggatg
     3961 gccagcggca agccgataac ggtgacaaca taactcagta gctcccaggt ggtgatggac
     4021 atcatgtgtc agtacgggtt gcgtttttaa acagcgtcag cgctaagtta actcactggc
     4081 gtgttgttga atcacatcta tgcactcatc aaactgcagt gacttatcga aaaaatactc
     4141 tacgcccatg ttttgcgcga ggtcgcggta atagctgttg ttgtgattgg tcagcatgac
     4201 aatatcaggt gtcttgaaag gataattctg ctgccttgtg tgacgcacga catcaaagcc
     4261 agtgcctttg gccagctcga tatccagcac gatcatgtcg tattgccgct tgttaagcaa
     4321 atcaatggcc tcgtcact
//
LOCUS       DATMSK010000034         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_449233,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000034 DATMSK010000000
VERSION     DATMSK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449233"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VNZ84_01150"
     CDS             <1..741
                     /locus_tag="VNZ84_01150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWU65585.1"
                     /translation="IAGRPSMGKTAFAINIAENVALDTNKAVAIFSMEMGATQLTTRM
                     IGSIGRLDQHKMRNGNLEDEDWEKLTTALGKLNEAPIYIDEGAGLSSFDVRARARRLH
                     RQTGELGLIVIDYLQLMTSPAGRSGENRATEISEISRSLKALAKELNVPLIAISQLNR
                     SVDQRPDKRPVMSDLRESGAIEQDADLIMFVYRDEYYNPDSAEKGTAEIIIAKQRNGP
                     IGRVRLTFMGAHTRFADYANAAYMPDEY"
     gene            760..1824
                     /gene="alr"
                     /locus_tag="VNZ84_01155"
     CDS             760..1824
                     /gene="alr"
                     /locus_tag="VNZ84_01155"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWU65586.1"
                     /translation="MRPIVAHISEAALAHNLAQVRRYAPQSKVIAVVKANAYGHGLLN
                     AARGLKETDAFAVLNISEAVALRDAGYQHPILLLEGVFSAEEMILVDRYHLDTVVSHQ
                     AQLAWVLESSPNIPLRVFLKLDSGMHRLGFQPHEYGHALQQLSGSANVRELILMTHFA
                     NADVADGADAAMQVVAQVMPSSYARSLANSAAIVQQPHTHADWVRPGIMLYGATPVAE
                     KSASSLGLRPVMTLQSGLIGVQEITAGESVGYGSLFAAEKPTRVGIVACGYADGYPRH
                     APTGTPVAVAGCLTRTLGRVSMDMLAVDISDIPEATLGSVVELWGEQVPVDDVAHASG
                     TIGYELLCAVTARVPFKVHG"
     gene            1817..3172
                     /gene="radA"
                     /locus_tag="VNZ84_01160"
     CDS             1817..3172
                     /gene="radA"
                     /locus_tag="VNZ84_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWU65587.1"
                     /translation="MAKAKTQYTCIECGAVEPKWQGQCPSCHAWNTLVETVPETATQS
                     RFASLAPTAQLQKLADVEAQDVSRQATGITEFDRVLGGGLVAGGVVLIGGDPGIGKST
                     LLLQTVCRVSQSLKAFYISGEESAQQIAMRAQRLGLDASQVDVLAEINLEKISHVLQH
                     HQPDVVVIDSIQTMYSEALQSAPGSVAQVRECSAQLTRIAKQLGISMILVGHVTKEGS
                     LAGPRVLEHIVDSVLYFEGDQNSSFRLIRAFKNRFGAVNELGVFAMTEKGLREVSNPS
                     ALFLSHHESPVPGSSITVTQEGTRPLLVEIQALVDTAHAPNPKRLCVGLEQNRLAMLL
                     AVLHRHAGVACYDQDVFINAVGGVKISEPAVDLAVLLAIVSSLKNKPLPSKLIVFGEV
                     GLAGEVRPVQRGQERLKEAAKLGFTHAIIPKANAPKGGVAGLHIEAVDRLEQAIDVLR
                     A"
BASE COUNT          708 a          785 c          960 g          767 t
ORIGIN      
        1 atcgccggcc gtccttccat gggtaaaacc gcttttgcga ttaacattgc cgaaaatgtg
       61 gcgctagata cgaataaagc cgtggccatt ttctctatgg agatgggcgc gacacagctg
      121 acaacccgta tgatcggttc gattggccgc ctggatcagc acaaaatgcg taacggtaat
      181 ctcgaagacg aagactggga aaaactgacc accgctttgg gcaaacttaa cgaagcgcct
      241 atttatattg atgaaggtgc tggtttaagc agttttgacg tgcgtgcacg tgcacgtcgc
      301 ttgcacaggc aaaccggtga attaggcttg attgtgatcg actatttgca gctgatgact
      361 tcacctgcag gccgatctgg tgagaaccgg gcgactgaaa tctccgaaat ttcacgttcc
      421 ctgaaagcgc ttgctaaaga acttaacgtg ccattgattg ctatttcaca gctgaaccgt
      481 agtgtggacc aacgcccgga caagcgtccg gtgatgagtg acttgcgtga gtcaggcgcg
      541 attgaacagg atgctgactt gatcatgttc gtgtaccgtg atgaatacta caacccggat
      601 agtgccgaaa aagggacggc cgagattatt atcgccaaac agcgtaacgg cccgattggc
      661 cgcgtgcgcc tgacctttat gggggcacat acccgtttcg ccgattacgc caatgcggcc
      721 tatatgccgg acgaatatta atcctgcttg gctctgcaca tgcgtcctat tgttgcccat
      781 atttcagaag ctgcgctggc tcataacctg gcccaggtca ggcgttatgc accgcaatcc
      841 aaagtcattg ctgtcgtcaa ggcgaatgcc tatggtcacg gcctgctcaa tgccgcgcgt
      901 ggcctgaagg aaacggatgc ctttgcggta ttgaatatca gtgaagctgt tgcattgcgt
      961 gacgctggct accagcatcc tatcctgttg ctggaggggg tgttctccgc agaagaaatg
     1021 atactggttg accgctatca cctggatacg gtggtctcgc atcaggccca gctcgcgtgg
     1081 gtgcttgaaa gcagccccaa tattccgctg cgggtatttt taaaactgga tagcgggatg
     1141 caccggctgg gctttcagcc gcatgaatat ggccacgcat tacagcagtt gagcggttct
     1201 gcgaatgtgc gtgaactgat cttaatgacg catttcgcca acgcagatgt ggctgatggg
     1261 gccgatgctg ccatgcaagt cgtggcgcag gtgatgccat cctcctatgc acgttcgctg
     1321 gccaattctg cggccattgt gcagcaacca catacgcatg cagattgggt gcgccccggc
     1381 ataatgttgt acggcgcgac gcccgttgcc gaaaaatcgg cgagcagtct gggcctgagg
     1441 ccagtgatga ccttgcaatc aggcctaatc ggagtgcagg agatcactgc aggcgagagt
     1501 gtagggtatg gtagcctgtt tgccgcggaa aaacctacgc gtgtgggaat tgtcgcttgt
     1561 ggctatgctg acggttatcc acgccatgca cctactggca cgccagtagc tgttgctggt
     1621 tgcttaaccc ggactttggg gcgggtgtcc atggatatgc tggccgttga tattagcgat
     1681 attcctgagg ctactcttgg tagcgttgtt gaactatggg gtgagcaggt gccggtagat
     1741 gacgtggcgc atgccagtgg caccataggc tacgaattgt tatgtgcggt cacggccagg
     1801 gtcccgttta aagttcatgg ctaaagcgaa aacacaatat acctgtattg agtgcggggc
     1861 cgtagagcct aaatggcagg gccaatgccc aagttgccat gcgtggaaca ctctggtgga
     1921 aacggtgcct gagaccgcga cgcaaagccg gtttgccagc ttggcaccga cggcgcaatt
     1981 gcagaaacta gctgatgtcg aggcgcagga tgtttctcgc caggcgaccg ggattactga
     2041 atttgaccgt gtgctgggtg gcggtctggt ggctggtggg gttgtgctga ttggtggtga
     2101 tcctggcatt ggtaaatcga ccttgttatt gcaaacggta tgcagggtat cccaatcact
     2161 caaagcattt tatatcagcg gtgaagaatc tgcgcagcag attgccatgc gggcacaacg
     2221 tctggggctg gatgcgagcc aggtcgatgt tttggccgag attaacctgg aaaaaatcag
     2281 ccatgtgctg cagcaccatc agccggacgt cgtggtgatt gattctatcc aaaccatgta
     2341 ttccgaagcg ttgcagtctg cacctggttc tgtggcacag gtgcgggaat gcagtgcgca
     2401 attgacacgg attgccaagc agttaggcat cagcatgatc ctggttgggc atgtgaccaa
     2461 agagggttcc ctggccgggc cgcgtgtgct ggagcacatt gtcgatagcg tgctctactt
     2521 tgaaggtgac cagaattcaa gtttccgttt gatccgtgcg ttcaaaaacc gctttggtgc
     2581 cgtcaatgaa ctcggtgtgt ttgccatgac ggaaaagggt ttgcgcgaag tcagcaatcc
     2641 ttctgccttg tttttatcgc accacgaatc gcctgtgcct ggttccagta ttactgtcac
     2701 ccaagagggt acgcgcccct tgctggtgga gatacaggcc ctggtggata ccgcccatgc
     2761 accgaatcct aaacgtctgt gtgtggggct ggagcaaaac cgtctggcca tgttgttggc
     2821 ggtgttgcac aggcatgccg gcgtagcctg ttatgaccag gacgtcttca tcaatgccgt
     2881 aggcggtgtc aagatcagtg aaccggccgt ggatttggcg gtattgctgg cgattgtgtc
     2941 ttcactcaaa aacaagccct tgcctagcaa gctgattgtg tttggtgagg tgggcttggc
     3001 aggcgaggta cgcccggtac agcgtggaca ggagcgcctc aaggaagcgg ccaagctggg
     3061 ctttacccat gccattattc ccaaagctaa tgcgcctaaa ggcggtgtcg ccggtttgca
     3121 tattgaggcg gtcgaccgac tggagcaagc gatagacgtg ctacgcgcat aaaaaaatgg
     3181 caacgactgc tgggagtgca atcgttgcct gagagtgagg
//
LOCUS       DATMSK010000035         6205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_143872,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000035 DATMSK010000000
VERSION     DATMSK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143872"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1215)
                     /gene="metK"
                     /locus_tag="VNZ84_01165"
     CDS             complement(52..1215)
                     /gene="metK"
                     /locus_tag="VNZ84_01165"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWU65588.1"
                     /translation="MSEYLFTSESVSEGHPDKLADQVSDSILDAILAQDPTARVAAET
                     LANTGLVVLAGEITTTANVDYINVARDAIKRIGYDNTDYGIDYKGCAVLVAYDKQSPD
                     IAQGVDNANDDPLDQGAGDQGLMFGYAVNETPQLMPLPIWLSHRVMERQSQLRKDGRL
                     PWLRPDAKSQVTIQYVDGKPYKIDTVLVSTQHAPEMSLEAIREAVIEEIIKPTLPADL
                     IKGEIKYLVNPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNIVAAGLADKCLVQVSYAIGVAQPTSIMVETYGTGKVSDERLTQLVRE
                     HFDLRPKGIVKMLDLLRPIYTKTAAYGHFGRDEPEFSWEATDKAAALRAAAGL"
     gene            1395..2225
                     /gene="dapF"
                     /locus_tag="VNZ84_01170"
     CDS             1395..2225
                     /gene="dapF"
                     /locus_tag="VNZ84_01170"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWU65589.1"
                     /translation="MKLRFTKMQGAGNDFVVIDATKNPVHLSHAQIQQIANRYFGIGC
                     DQLLMVESTDTPGVDFRYRIFNADGGEVEQCGNGARCFVRFVVDKGLTSKRDIRVETA
                     SGIITLMLQDDGQVTVNMGAPRFTPAQIPFEAEQQATTYELPLASETVTVSAVSMGNP
                     HAVMLVDNVHTAEVASLGPQIESHARFPQRVNAGFMQVLNEHEINLRVYERGSGETLA
                     CGTGACAAAVSGIQLGTLQSPVKVHTRGGILTIQWAGGDTPVLMTGPAEIVFDGEIEI
                     "
     gene            2243..2896
                     /locus_tag="VNZ84_01175"
     CDS             2243..2896
                     /locus_tag="VNZ84_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HWU65590.1"
                     /translation="MEENINENDIKHYLKAHPDFFERHAHLLAEMFLPSPHGKGTISL
                     AERQQIAQRDKIRVLESRFTELILTAEENEATAQKVHELTLGLLYAKDVTSLHTHVVD
                     FLSVHFQLPNAQLKLWTNESHAADHLFIEAEESLKNWAKDLSQPYCGTLPTMDIAGWF
                     TDTPASLAVIPMRYQEVTFGLMAIPSEQRSRFYAGMGTLFLNQIGELVSASLARYII"
     gene            2978..3859
                     /gene="xerC"
                     /locus_tag="VNZ84_01180"
     CDS             2978..3859
                     /gene="xerC"
                     /locus_tag="VNZ84_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HWU65591.1"
                     /translation="MHELLDDYLDFLHFERGLSEHTRNNYQRDIERLLHTSTQDLQSL
                     TPQDIRRQIASLHGQGMHGRSIARMLSSWRGFFRYLVLHKGFPSNPVTGLRAPKAAKT
                     LPHALSIEQTNKLVEIESEEPIALRDRAILELFYSSGLRLSELVGLNLDRLDLHEGLI
                     TVIGKGNKTRMVPLGSKAVSAMKDWLAVRSLWLVKRPSETAVFVSQQGRRIHARTVQT
                     RINHWVIKQGLHNHVHPHMLRHSFASHVLQSSGDLRAVQEMLGHANISTTQVYTHLDF
                     QHLSKIYDAAHPRARKK"
     gene            4028..4612
                     /locus_tag="VNZ84_01185"
     CDS             4028..4612
                     /locus_tag="VNZ84_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HWU65592.1"
                     /translation="MEHVLPALPFAKNALAPHMSEETFDYHYIKHHQAYVTNLNNLIK
                     GTEFENATLEDIIKKSSGGIYNNSAQVWNHTFFWNCLSPNGGGEPSGALAAAINAKWG
                     SYEEFKKAFQASAVGNFGSGWTWLVKKADGSVDIVNTGAAGTPLTTGDTALLTIDVWE
                     HAYYIDHRNARPKFVETFLNHLVNWEFAAKNFAA"
     gene            4883..5362
                     /locus_tag="VNZ84_01190"
     CDS             4883..5362
                     /locus_tag="VNZ84_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWU65593.1"
                     /translation="MTTTPASRPPLPPFTRETAIQKVRLAEDGWNSRDAEKVALAYSI
                     DTNWRNRSEFIKGREEAQKFLERKWNKEHEYRLIKELWAFDGNRIAVRYAYEWKDDSG
                     NWFRSYGNENWEFNPDGLMVNRYASLNDVPIKESERLFHWPLGRRPDDHPGLSALGL"
     gene            complement(5418..5972)
                     /locus_tag="VNZ84_01195"
     CDS             complement(5418..5972)
                     /locus_tag="VNZ84_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU65594.1"
                     /translation="MIENEPLQRLLAATEKLIYANGINATGMEAIVNASGVARKTIYR
                     HFPTKEDLIAEVLRQRDQRWMQWFIAFTSAMTEPKARILASFDALEEWFLTEGFNGCA
                     FINAAGECGSTSTLIAAVAKEHKVNLAHYLEQLAAAYGVDEPKKMAADLLLLIEGAIT
                     VAKVMGDKAAAKNGQRLAEQLLLQ"
BASE COUNT         1669 a         1602 c         1441 g         1493 t
ORIGIN      
        1 tcaaaattac atttatatga atgttttttt aaacgctggt catgcacacc atcacaggcc
       61 ggcagctgca cgcaaagctg cagccttatc tgtggcttcc cagctaaact ctggctcatc
      121 acggccaaaa tggccataag ccgccgtttt agtgtagatg ggacgcaata gatccagcat
      181 tttgacaatg ccttttggac gcaggtcgaa atgctcgcgc accagctgcg tcagacgctc
      241 atcagaaact ttgccagtac cgtaagtttc caccatgata gaggtaggct gagccacacc
      301 aattgcatag cttacttgta ccaagcactt gtctgccagg cctgcggcaa caatattctt
      361 ggcaacgtaa cggccagcat aagccgcgga acggtccact ttggatggat ccttcccaga
      421 aaaagcacca ccaccgtgag gggctgcacc accataggta tccacgataa tcttacggcc
      481 tgtcaggcca caatcgcctt gcggaccacc aatcacgaaa cggccagtcg ggttaacaag
      541 gtatttaatc tcgcccttaa tcaagtctgc aggtaaggtt ggcttgatga tttcttcaat
      601 cacagcctcg cgaatcgctt ccagtgacat ttctggcgcg tgctgggtag acaccagcac
      661 cgtatcaatt ttatatggct tgccatctac atattgaatc gtcacttgtg attttgcatc
      721 tgggcgcaac catggcaaac ggccatcctt acgcaattgc gactggcgct ccatcacgcg
      781 gtggctcaac caaataggca atggcattaa ttgtggcgtt tcattcacgg cataaccaaa
      841 catcaagccc tggtcaccgg ccccttgatc cagcggatca tcattggcat tatccacgcc
      901 ttgagcaatg tcaggagatt gcttatcgta ggccaccaat accgcgcaac ctttgtaatc
      961 aatcccgtag tcggtgttgt catacccaat gcgcttgatg gcatcccggg caacgttgat
     1021 gtaatccaca ttcgcagtgg tagtaatttc acccgccaat acaaccaggc cggtatttgc
     1081 cagcgtttcc gcagccacac gtgcagttgg atcctgggcc agaattgcat ccagaatgct
     1141 gtcagagacc tgatcagcta atttatcagg atgtccttcg gaaaccgatt ccgaagtaaa
     1201 aagatattca ctcatgtttg acgccttgaa aagtaataaa gttaataaat ggcactgact
     1261 aaaggctaaa gccagcaccc aattggggaa cgaagattct acccgagttt atagtagcca
     1321 aggaataaat ctgcgccctc taaattttca gctagagatg ccttcaacgg cgcgatagag
     1381 tatcatcttg agctatgaaa ttacgattta ccaaaatgca gggggcaggt aatgactttg
     1441 tagtcattga tgccaccaaa aatccagtcc atctatcaca cgcgcaaatc cagcagattg
     1501 ccaaccgcta cttcggtatt ggttgtgacc agttgctgat ggttgagtct acagacacac
     1561 ctggcgtcga cttccgctac cgtattttca atgcagatgg cggcgaagtg gaacaatgcg
     1621 gtaatggtgc acgctgtttt gtgcgctttg tggtcgacaa aggcctcacc agcaaacgtg
     1681 atatccgcgt cgaaacggcg agcggcatca ttacactgat gctacaagac gacggccagg
     1741 tcaccgttaa tatgggcgca ccgcgtttta caccagcgca aattccgttt gaggctgaac
     1801 aacaagcaac cacttatgaa ttgcctctgg ctagcgaaac tgtcacagtt agcgccgttt
     1861 caatgggcaa cccgcatgcc gtcatgctgg ttgataatgt ccatacggcc gaagtggcca
     1921 gcctggggcc gcaaattgaa tcacacgcgc gcttcccgca acgcgtgaac gcaggtttta
     1981 tgcaggtgct caacgagcat gaaatcaacc tgcgcgttta tgaacgtggc agcggtgaaa
     2041 ccctggcatg cgggacaggc gcttgcgccg ccgccgtcag cggtatccag ctggggacct
     2101 tgcaaagccc ggtcaaggtg catacccgtg gtggtatcct gaccatacaa tgggcaggtg
     2161 gcgacacacc ggttctgatg acagggcctg cagaaattgt gtttgacggt gaaattgaaa
     2221 tttaggtaaa gacagcagca gcatggaaga aaacatcaac gaaaacgata tcaagcatta
     2281 cctcaaagca catcctgatt tctttgagcg tcatgcacac ctgctggcag aaatgtttct
     2341 gcctagccca catggtaaag gcaccatttc gctggccgaa cgccagcaaa tcgcccagcg
     2401 cgacaaaatc cgcgtactgg aatcacgctt taccgagctg atcctgactg ctgaagaaaa
     2461 tgaagccaca gcacagaaag tgcatgagct gactttgggt ttactctatg ccaaggatgt
     2521 gacctccctg catacacatg tagtagattt tctgtccgta cacttccagc tacccaatgc
     2581 gcaactcaaa ttatggacaa acgaaagcca tgctgcagat catctcttta tagaggcaga
     2641 agagtcactc aaaaattggg ctaaagacct gagccagccc tattgcggca ccttacctac
     2701 catggatatt gcaggctggt ttaccgacac acccgcctcg ctcgcagtca tccccatgcg
     2761 ttaccaggaa gtcacgtttg gcctgatggc gattcccagt gagcaacgca gtcgctttta
     2821 tgccggcatg ggtaccttgt tcctcaacca gatcggtgaa ctggtaagcg catcactggc
     2881 cagatatatc atatgaaagc ccccgttgag atttccgcca agaggtattt tcttgcggca
     2941 attgtcagcg taaagccccc tgatagaggt agtttggatg catgaactgc tagatgacta
     3001 cctcgacttc ctgcacttcg aacgcggcct tagcgagcat acccgcaaca attaccagcg
     3061 cgacattgag cgcttgttac acacttcaac gcaagacctg caatcactca ccccgcaaga
     3121 catccggcgc cagatagcca gtttgcacgg tcaaggcatg catggtcgca gcatcgcacg
     3181 catgttgtcg agctggcgtg gttttttccg ttacctggta ctgcacaaag ggtttccgtc
     3241 taatccggtg accggactgc gcgcacccaa agcagccaaa actttaccgc atgccctgtc
     3301 aattgaacaa accaacaaac ttgttgagat agagagtgaa gagccgattg cactgcgtga
     3361 ccgcgccata ctggagctgt tctactcctc tggcctgcgt ctcagtgaac tggttggctt
     3421 gaatttggac cgcctggact tgcacgaagg tctgattaca gtgattggta aaggcaataa
     3481 aacccgcatg gtaccgttgg gcagcaaagc agtgagtgca atgaaagact ggcttgcagt
     3541 gcgtagctta tggttggtca aacggccttc agaaacagcc gtgtttgtca gccagcaagg
     3601 gcgccgcatc catgcccgca cggtgcaaac acgcatcaat cattgggtca ttaaacaggg
     3661 attacacaat cacgtacatc cacatatgct gcgtcacagc tttgccagcc acgtgttgca
     3721 aagcagtggc gatttgcgtg cagtgcagga aatgctgggc cacgccaata tcagtaccac
     3781 tcaagtctac acccatctcg acttccagca cctgagcaaa atttacgatg cagcgcatcc
     3841 gcgagcacgt aaaaaatagc gtatttatat tcactgcctc ttaagcaagg ttttgcaaga
     3901 cggcaaaata tggcaaaata acctgactaa attggtaaac ctttgccagt ctcgtgactc
     3961 caatgtcata tcgcctgagc ttacaaaagc aggcaattag ttcaacaaac caaaaaagga
     4021 accttccatg gaacacgtct taccagcatt gccatttgca aaaaatgcat tggcccctca
     4081 tatgtcagaa gagacttttg actaccacta catcaagcac caccaggctt atgtgaccaa
     4141 cctgaacaac ctgatcaaag gcactgagtt cgagaacgcc actctggagg acatcatcaa
     4201 gaaatcttca ggcggcattt acaacaactc tgcccaagta tggaaccaca cctttttctg
     4261 gaactgcctg tccccaaatg gcggcggcga accatcgggc gcactggcag cggccattaa
     4321 tgccaaatgg gggtcttacg aagagtttaa aaaagctttc caagcctcag ccgttggtaa
     4381 ctttggttct ggctggacct ggctggtgaa aaaagccgat ggctctgtcg atatcgtcaa
     4441 caccggcgcg gctggcacac ctttgaccac tggtgatacc gcattgctga ccatcgacgt
     4501 gtgggaacat gcctattaca tcgaccaccg taatgcacgt ccgaaatttg tcgagacatt
     4561 cctgaaccac ttggtgaact gggaatttgc agccaaaaat tttgctgctt aagctctata
     4621 gataatttaa aagtcttttt gactgttgaa tagcccaaag gcctctgcct ctcataggag
     4681 aggcctttgt cattttgtgt cctggtcttt tgagagtgat cccatattta aatggcactt
     4741 catgattgac atcaaagaac aaccgttcta ccataagtag aacaatcatt ctacctaaag
     4801 aataccgatc aatattggca ttcattcaga aatgcttgga agtttgaaac atatatcacc
     4861 aacttgagta tagggagtag ttatgacaac aacaccagcg agcaggccac cactgccacc
     4921 gtttacacgt gaaactgcca tccagaaagt caggttggcc gaagatggct ggaatagccg
     4981 tgatgcagaa aaagtggctt tggcctactc tatagatacc aattggcgca accgctctga
     5041 gttcatcaaa gggcgtgaag aggcgcaaaa attccttgag cgtaaatgga acaaggaaca
     5101 tgaatatcgg ctaattaaag aattgtgggc ttttgatggc aaccgcatcg cagtcaggta
     5161 tgcctatgaa tggaaggacg attccggaaa ctggttccgc tcgtatggaa atgaaaactg
     5221 ggagtttaat cctgatgggc tcatggtcaa ccggtatgca tccttaaatg atgtgcctat
     5281 caaagagagt gagcgcttat ttcattggcc actgggcaga cgtccagatg accacccagg
     5341 tttgtcagcg ttaggcttgt aaatcatgag ggatggttta aaccggccct catgatttaa
     5401 gaattcagtt aacgttgcta ctgcaataac aactgctcag ccagccgttg gccatttttt
     5461 gccgcggctt tgtcccccat caccttggcc acggtaatgg caccctcaat caacagcaat
     5521 aaatctgccg ccatcttttt gggctcgtct acaccatacg ccgctgccag ctgctcaaga
     5581 taatgcgcta aatttacttt atgctctttg gcaacagcag cgatcaatgt gctggtactg
     5641 ccgcactcac ccgcagcatt gataaatgca cacccattaa aaccctcggt caaaaaccat
     5701 tcttctagcg catcaaatga cgccagtatg cgtgcttttg gctcggtcat cgcactggta
     5761 aaggcaataa accattgcat ccatctttga tcccgttgcc ttaagacctc ggcaatcagg
     5821 tcctctttgg tgggaaaatg gcgatagatc gttttgcggg ccacgccgga cgcattcacg
     5881 atggcttcca tcccggtcgc attgatgccg ttggcataaa tcaatttttc agtggcagcg
     5941 agtagccttt gcagaggttc attttctatc atgctcatca ttgtagaaca gtcgttctac
     6001 aactgtccat acatactacc tttaaggcta ttgtttcgat agacgcaata atgaattgcg
     6061 ggtagcctgg attttcaact ctaatgaatc agttaaattt caatcacacc gtcagtgcga
     6121 cggagccagc tgaaataaat gattgaatct aactattgag aggactgatg atgaaaaatg
     6181 cgacttttaa atggatggta cgcgc
//
LOCUS       DATMSK010000036         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_285437,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000036 DATMSK010000000
VERSION     DATMSK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285437"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VNZ84_01200"
     CDS             <1..263
                     /locus_tag="VNZ84_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65595.1"
                     /translation="VNVKPVTFHRWLSGKLAAPENKLEKIKSCAFADFRRRPRKIAHL
                     SQVAEADVEVIRAKFLWKNMLFDQLHMTAKRRYCAGLTRLHR"
     gene            complement(322..686)
                     /gene="ssrA"
                     /locus_tag="VNZ84_01205"
     tmRNA           complement(322..686)
                     /gene="ssrA"
                     /locus_tag="VNZ84_01205"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            768..1637
                     /locus_tag="VNZ84_01210"
     CDS             768..1637
                     /locus_tag="VNZ84_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HWU65596.1"
                     /translation="MFKKIVIFGVGLIGGSVALALKKQPHPPQIIGVGRSNQSLQEAF
                     QLGLIDSAETDIAAALQDADLVFLATPVAQTPTILRAICPYLNSSTIVTDAGSTKSDV
                     MAYAKAELGDKVSQFVGGHPIAGAEKSGPSAALADLYTGKNVILTPDAETATEALEKV
                     RTLWQQCGAVVSCMSAQEHDGVFAAVSHLPHLLAFALVEDLAKRDNAELLFKFAASGF
                     RDFTRIAGSHPEMWRDIALANKHALLTELNMYQQAINEMTALLEQDNGDALHALFEHA
                     SRARNDWAKSKIQ"
     gene            1647..>2205
                     /locus_tag="VNZ84_01215"
     CDS             1647..>2205
                     /locus_tag="VNZ84_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWU65597.1"
                     /translation="MEQLHLPAAHQAHGTITLPGSKSISNRTLLLAALAEGKTVIRDL
                     LASDDTARMLEALTALGIQLENIGENAWQVTGCGGRFPNKHADLFLGNAGTAFRPLTA
                     ALAFSNGEYHLHGIPRMHERPIGDLVDALKQAGARVAYLANDGYPPLQISPAQLDVSQ
                     PIKIRGDVSSQFLTALLMALPLTGQA"
BASE COUNT          558 a          537 c          547 g          563 t
ORIGIN      
        1 tggtgaatgt gaaacctgtc acttttcatc gctggctttc tggcaagtta gcggcgcctg
       61 agaataaact ggagaaaatc aaaagctgtg cttttgccga cttcaggcgc cggcctcgaa
      121 agatagcgca tctcagtcag gttgctgaag ctgatgttga agtaatcagg gcaaaatttt
      181 tatggaaaaa tatgcttttt gatcaattgc atatgactgc aaaacgtaga tactgtgctg
      241 gcttaacaag gttgcatcgg tagcttcaat gcagttaaag taaataaaaa gaccacttca
      301 acatgaagtg gtctttttat ttggtggagc tggggggaat tgaacccccg tccgcaagcc
      361 ctccacagac agttctacat acttagctgt gttgtttatg tttaaccagg cacccaccaa
      421 cgagcaagca gatgtctagc gagttgcctt agatttaatg ccttaccaag caacccggta
      481 aaacacgatc ctctctaaat gacgctgctg ccggttaccc ggcccgaccg agaggctctt
      541 cggggcagcg tccggctgga ttaagcagcc agagcgtaag tttcgtcgtt tgcgactata
      601 cttgttcaga tggatttacg agggaacctg aatcctcggt atgccctgca ctgctttgca
      661 acccacgtcg aaaccaggtc agccccaatg caatcttgcg attatacgcg ctaaaagctg
      721 gcgagccaaa ctgagagcgc atttatatga catggtacaa ttccgacatg ttcaaaaaaa
      781 ttgtcatttt cggggtggga ttgattggtg gctcggtcgc attagcactg aaaaaacagc
      841 ctcatccgcc acaaatcatt ggtgtcggtc gtagcaatca gagtttacaa gaggcctttc
      901 aactcggctt gattgatagc gcagaaactg acattgccgc agcactgcaa gatgcagatt
      961 tggtattcct tgcaacacct gttgcccaaa cgccaaccat tttgcgcgcc atatgcccgt
     1021 atctgaatag ctctacgatt gtcacagatg ctggcagtac caaatctgat gtcatggctt
     1081 atgcgaaagc tgagcttggc gataaagtca gccaattcgt cggtggacat cctattgccg
     1141 gtgctgagaa aagtggtcca tctgccgcgc tggcagattt atatacaggt aaaaatgtca
     1201 ttttgacgcc tgatgcagag acggcaactg aagccttgga aaaggtgcgg acattatggc
     1261 agcaatgtgg tgccgtggtc tcctgcatga gcgcgcagga gcatgatggt gtgtttgctg
     1321 cagtcagtca cctgccccat cttttggcct ttgcccttgt cgaagaccta gccaagcgtg
     1381 acaatgccga gttgctattc aagtttgctg ccagtggatt ccgtgacttt acacgcattg
     1441 ctggtagcca tcctgaaatg tggcgagata tcgcactggc taataaacat gccttgttaa
     1501 ccgagctaaa catgtatcag caggccatca atgaaatgac agcgctgttg gagcaggata
     1561 atggcgatgc attacatgcc ttgtttgagc acgccagccg tgcacgtaat gactgggcaa
     1621 aatctaaaat tcaataatta ttcactatgg aacaactgca tttacccgct gctcaccagg
     1681 cccacggcac gattacctta cctggatcca aaagtatttc taaccgcaca ttattgcttg
     1741 cagccttggc ggaaggcaaa accgtgattc gcgatctgct ggcttctgat gatacggcgc
     1801 gtatgctgga agccctgact gcactaggta tccagctcga aaacataggc gaaaatgcct
     1861 ggcaagtaac aggttgtgga gggcgtttcc ctaacaagca cgcggatctg tttttgggca
     1921 atgcaggtac cgcctttaga cctttaactg ctgcgctagc attttcaaac ggtgaatatc
     1981 acttgcatgg catccctcgc atgcatgagc ggccgattgg cgatttggtt gatgcattaa
     2041 agcaagctgg cgcccgggtg gcttacctgg ccaatgacgg ctaccctccc ctgcagattt
     2101 ctcccgccca actggatgtt agtcagccga ttaaaatccg cggggatgtt tctagccagt
     2161 ttttaacggc tttactcatg gccttgccgt taaccggaca agccg
//
LOCUS       DATMSK010000037         4746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_27001,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000037 DATMSK010000000
VERSION     DATMSK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27001"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VNZ84_01220"
     CDS             <1..352
                     /locus_tag="VNZ84_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWU65598.1"
                     /translation="GAVILLAMEWRTWWFYLQPPPDFSHGQVMLYATDWCPYCEKTRA
                     LLASRGIPYREMNIENSAEARQQYQRLAANGVPVLLIAGDVVRGYNPKKMEDLLDAWQ
                     KAQTPGNAMSHGNY"
     gene            complement(547..1713)
                     /locus_tag="VNZ84_01225"
     CDS             complement(547..1713)
                     /locus_tag="VNZ84_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HWU65599.1"
                     /translation="MSAISTRSVLVMTIVAMLLIPLAASWFAYPSTHLPPGFGIFPPQ
                     FVEDPPGFNLIVFIALALLEAAFAVFLIFPQWFGFRPVTPAPQPALRALPWWFWVGAV
                     VTLFFWWLMWTRTTPFGDLVYYAFTPMWWGFIFVLDGLAYRYSGGYSLFASRPKTFLI
                     SAAVSIGGWYFFEYFDYFALGNWYYPNTDIPGLSHTTIVWIFIFAYTTVWPAIFEWYT
                     LLNAFPKIAARYAQGPALALPAKPMLILSFVLFAAVPFWPYPLFWAMWIAPLIGIAGI
                     LMMCGIWTPFADLAKGNWSPVLLMALSSLFNGFFWEVWNYGSAHPVLPVTNPNYWVYD
                     IPYVNVIHIDAEMPLLGFAGYLPFGVLVWVVFILAGKVFGFNTDLLNSSQRQSR"
     gene            complement(1768..3354)
                     /locus_tag="VNZ84_01230"
     CDS             complement(1768..3354)
                     /locus_tag="VNZ84_01230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWU65600.1"
                     /translation="MIALLSIGLAQAAPQSIKGAWSLVHPFDRAQGDHQFLAEPLHQQ
                     YPPVPAVSLTGGHYLYVAPFEISNKGEYVFDFKNTSTIEHFRHQIYDQQGREVAILEG
                     GIGRRIENPFFLRHGRSVSLMPGKYVLATQLISTNYLAIPEPYLDDQASYMHAIRYGN
                     ALTIFCLGIFWGLGFYYTALAASRNRIVEAMYAIFIAGNFIYNGAALLVLSDLLNVHS
                     IYLVSMPVLISNIAYMLFVMHLLEINRYQQKPLYYVGMSILAGMVSFLLMAIAFPNWA
                     LEFCRYGVGLFLCYGLAAAIRQSQRKNATAKRYLIAISLFFVSGILAISLSKLNQHFD
                     LYIEHLGLVSVAIEVVLLALVLSFQFSQLHKEKEEALEALGMTEKVASSDALTSLPNR
                     YAFVKALEQLTMQSSLTFIDLDGLKLYNDYYGHARGDELLVCFAKNCQQQLGAHLALY
                     RMGGDEFAVLSLKGEVVAVERALQEAIERVRSEGFEFSGASAGSAYYHEAENISEFMR
                     LADMRMYENKRSRQLRAATA"
     gene            complement(3614..3978)
                     /gene="rnpB"
                     /locus_tag="VNZ84_01235"
     ncRNA           complement(3614..3978)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VNZ84_01235"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4046..4732
                     /gene="ung"
                     /locus_tag="VNZ84_01240"
     CDS             4046..4732
                     /gene="ung"
                     /locus_tag="VNZ84_01240"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWU65601.1"
                     /translation="MTIQEKPGQLDPSWQTVLGAEFEKPYMQSLKAFLQQEKAAGKTI
                     FPPGPLIFNAFNHTPFDKVRVVIIGQDPYHGPNQAHGLSFSVQAGVALPPSLLNIFKE
                     IQSDLGIRMSGNGDLTPWADQGVLLLNATLTVQMANAGSHQNRGWETFTDAAIAALNA
                     QREGLVFVLWGSYAQKKGAMIDPNKHLILKSVHPSPLSAHRGFFGTRQFSKINDYLIS
                     RGQTPIHWQL"
BASE COUNT         1365 a         1290 c         1084 g         1007 t
ORIGIN      
        1 aggcgcagtt atcttgctgg ccatggaatg gcgcacgtgg tggttttacc tgcagccgcc
       61 accagacttt agccatgggc aggtgatgct ctacgccacc gattggtgcc cctattgcga
      121 aaaaacacgc gccctgcttg caagcagagg gatcccgtat cgcgaaatga acatcgaaaa
      181 ctcagcagag gcgcgccagc aataccagcg cctggccgcg aacggtgtcc ccgtattgct
      241 gatcgcaggt gacgtggtac gtggttataa ccctaaaaaa atggaagacc tgctggatgc
      301 atggcaaaag gcccaaaccc caggcaatgc catgtcgcac ggaaactact aaaacagact
      361 gaagggcaga gggatgattg agctagcgcg acaagatcac tatcgaagaa ttgtttttat
      421 agccctgcca gcagcagcct gtcagattta atgacgacca cagtttcatt ggcgaaacac
      481 cgaagtgagc cacggtgttt cgcctgctga ctccgccaga gcgtagtcgc ggaatggata
      541 catgcgttag cgcgactgcc tttgactact gttaagcagg tcagtattaa aaccaaacac
      601 tttacctgcc aagataaaca cgacccacac cagcacacca aaaggcaaat agccagcaaa
      661 acctaatagc ggcatttcag catcaatatg aatgacattg acataaggaa tatcgtaaac
      721 ccaataattc gggttggtga caggtaatac cggatgggcg ctaccgtagt tccatacctc
      781 ccagaaaaaa ccattaaaca gggaactgag cgccatcaag agcacaggac tccagtttcc
      841 cttggcaaga tcagcaaatg gtgtccagat accgcacatc atcaagatgc cggcgatacc
      901 tatgagcggt gcaatccaca tcgcccagaa caaaggataa ggccagaatg gcacggcagc
      961 aaacaacaca aaactcagga tcaacatagg ctttgcaggc agcgccaatg ccgggccttg
     1021 tgcataccgt gcggcaattt taggaaaagc attcagtagc gtataccact caaaaatggc
     1081 aggccaaacg gtggtatagg cgaaaatgaa aatccacaca atcgtggtgt ggctcaaacc
     1141 gggaatatcg gtattggggt aataccagtt acccaaagca aaataatcga aatactcaaa
     1201 aaaataccag ccaccaatag atacagccgc actgatcaga aatgttttgg ggcgactggc
     1261 aaaaagggag tagccaccag aatagcggta tgccaggcca tcaagtacaa atataaatcc
     1321 ccaccacatc ggggtaaaag cgtaatacac caaatcacca aacggtgtcg tccgtgtcca
     1381 catcaaccac cagaaaaaca gtgtcaccac tgcgcccacc caaaaccacc agggcaatgc
     1441 ccttagcgcg ggctgcggcg ccggggtaac tggcctaaag ccaaaccatt gcgggaatat
     1501 taaaaaaacc gcgaatgcag cttcaagcag ggccaacgca ataaaaacaa tcaggttaaa
     1561 ccctggcgga tcctcaacaa actgcggtgg gaaaatacca aaacctggcg gcaaatgcgt
     1621 cgatggataa gcaaaccagg acgcggccaa tggaattaat aacatcgcca caatggtcat
     1681 cactagcacc gaacgtgttg aaattgcaga cataagctcc cctttgattt gtgacgaatt
     1741 gcgcccaata atgagccttt ataaatatta tgcagtcgcg gcgcgcaatt ggcgtgaccg
     1801 cttattttcg tacatgcgca tatcggccaa acgcatgaat tcagagatat tctccgcctc
     1861 atgataatag gcagagcctg cgctggcgcc agaaaactca aatccttcac ttcgtacacg
     1921 ttcaatcgcc tcttgcagtg cgcgctcaac cgcaacaact tctcccttta ggctaagtac
     1981 tgcaaactcg tccccgccca ttcgatacaa agcgagatga gcacccaatt gctgctgaca
     2041 gttctttgca aaacaaacaa gcaactcgtc gccacgcgca tgtccatagt aatcattgta
     2101 gagtttgagc ccatccagat cgataaatgt caggctgctc tgcatggtca gctgctccag
     2161 cgcctttaca aaagcatatc gattcggcag gctggttaag gcatccgaac tcgccacttt
     2221 ctccgtcatt cccagcgctt ccaacgcctc ttctttttct ttgtgcaact gggagaactg
     2281 aaaagaaagc actaatgcca gcaacaccac ctcgattgcc acactcacca accccaggtg
     2341 ctcgatatac aagtcaaaat gctgattaag tttgctgaga ctgatcgcga gaattccaga
     2401 gacaaagaac aggcttattg caatcagata gcgcttggct gttgcatttt tccgttgact
     2461 ttgcctgatc gccgctgcca gcccatagca taaaaacaag ccaacaccgt aacggcaaaa
     2521 ctccaaagcc caattgggga acgcgatggc catcaataga aacgacacca tccccgctaa
     2581 aatactcata cccacgtagt aaagcggttt ttgctgatac cgattgattt ccaataaatg
     2641 catcacaaat aacatgtagg caatattgga tatcaataca ggcatcgaaa ccaggtaaat
     2701 ggagtgtaca ttgaggagat cagagaggac gagcaacgct gcgccgttat aaatgaaatt
     2761 gcctgcaata aaaatggcat acatggcttc cacaatccgg ttgcggcttg ccgccagcgc
     2821 agtatagtag aaccccaaac cccaaaatat tcccaggcag aagatcgtga gcgcgttccc
     2881 atacctgatc gcatgcatat agcttgcctg gtcatccaga tagggctcgg gaatcgccaa
     2941 atagttggta gaaatcagtt gcgttgccag gacatacttt cccggcatca ggctgacact
     3001 gcggccatgc cttaagaaga aaggattctc gatgcgacga ccaatcccgc cttccaggat
     3061 cgccacctca cggccttgct ggtcatagat ctggtgtcgg aaatgctcaa tcgtactggt
     3121 attcttgaaa tcaaaaacat actcgccctt gttgctgatt tcgaaaggcg caacatacag
     3181 ataatgccca cctgtcagac tcacagcggg aacaggggga tattgttgat gcagcggctc
     3241 agcaagaaac tgatggtcgc cttgcgcacg atcaaacgga tgcactagag accaggcgcc
     3301 cttgatggac tgaggagcag cctgcgccaa gccaatggac agcaaagcga tcatgaccgc
     3361 taccagcctc ctgcccaagc tactcacctt ccccatgatc caatcaccgc tttttattaa
     3421 ttaatacgca aactatacaa gaaagtcacg ccatcgccca gtttaaccaa tcatgcttcc
     3481 acgcaaaccg gaacgtcttg gacttccgac cgacatattg agcagatgca acccataggg
     3541 aaaaacgggc agtcacgccg catttgatat cagggctagg aagagccgtc tgaaaagatg
     3601 gcaaggtgag agaaaataaa gccaaccggt aagccgggtt ctgtatccac ttgcgtggcg
     3661 acagtcattc ctctaggcgt gcaattactc acacgctcaa gctatctacc cgctggcagc
     3721 gcgagccacg ccttattact tgcgtaaaat gccagcctat ttgatattgc tgcagatgga
     3781 ggttaccgcg tttcacccgt ttcgacgcaa gttcgcaaca agttgctcac tggtcgaacc
     3841 gactcgtctc tgtggcccta ttcctcgcct tatactgtta ccagctttca gcgtacggcc
     3901 gttagccgtc atcccgctct gtgcagcccg gactttcctc cccctgtttg cacaggcggc
     3961 gactgtctgg ttggcttcct ggtcatttta ccacgcatgg cctaacaccc tataatgacc
     4021 aaaaaaatca ttcacctgac cactcatgac cattcaagaa aaaccgggcc aactcgatcc
     4081 ttcctggcaa accgtgctcg gcgcagaatt tgaaaaacct tacatgcaat cacttaaggc
     4141 ttttctgcaa caggagaaag cggcgggcaa aaccattttc ccgcctgggc cgctgatctt
     4201 caatgcgttc aatcacacgc cctttgacaa agtgcgcgtc gtgattattg gccaggaccc
     4261 ttatcacggc ccgaaccagg cacatggttt gagcttttcc gtgcaagcgg gcgttgcatt
     4321 accgccgtca ctgctcaaca tttttaaaga gattcaatct gatttaggca tacggatgag
     4381 cggcaatggc gacttaacgc cttgggcaga ccagggcgta ttgttactca atgccacatt
     4441 aaccgtgcaa atggcaaatg ctggctcaca ccagaaccgc ggctgggaaa cctttaccga
     4501 tgcggcaatt gctgccctta atgcacagcg ggaaggcctg gtattcgtgc tgtgggggag
     4561 ttatgcgcaa aagaaagggg ccatgataga ccccaataaa cacctgattt taaaatctgt
     4621 gcatccgtca cctttatcgg cacaccgtgg cttctttggc acacgccagt tttcaaaaat
     4681 caatgactac ctgatttcgc gaggacagac gccgattcac tggcaacttt agacttaagg
     4741 cttcac
//
LOCUS       DATMSK010000038         7843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_426057,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000038 DATMSK010000000
VERSION     DATMSK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426057"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VNZ84_01245"
     CDS             <1..1337
                     /locus_tag="VNZ84_01245"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HWU65602.1"
                     /translation="RKYQTFGGIILSASHNPAGPDDDFGIKYNTGNGGPAPEKITDAI
                     FANSKQITQYKLLDLPDVDLSSIGVTTLDGFEVEVIDSVSDYADLMQSMFDFSAIKRW
                     LAAGHRLQFDAMHAVTGPYAKEIFVNRLGAPASSLMNCEVSENFGGGHPDPNLTYAED
                     LVKIAYGPQAPDFSAASDGDGDRNMILGNHFFVTPSDSLALIAANAKLIPAYAQGLSG
                     VARSMPTSGAVDRVAEKLGIDCYETPTGWKFFGNLMDAGRVTLCGEESFGTGSDHVRE
                     KDGLWAVLCWLNLLAATGESVESLVRKHWQTYGRNVYSRHDYEGVPTEQANAVIAHIK
                     AQFNSLPGQVFGSYTVKTVDDFSYTDPVDGSVSTGQGLRILFACGSRVVIRLSGTGTE
                     GATIRIYLEAFTEDAAQQQLDAQEALAELIAVSHQISQLTQLTGRQQPTVIT"
     gene            1373..2926
                     /locus_tag="VNZ84_01250"
     CDS             1373..2926
                     /locus_tag="VNZ84_01250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWU65603.1"
                     /translation="MIKKTILCPVLLSAGLLACGVNPVTKEREFQFVSQDKEVQIGQQ
                     NYSPARQSQGGDYTLDNELTRYVQQVGQRLAAVSDRPDLPYEFVVLNDSVPNAWAMPG
                     GKIAFNRGLLYELHSEAELAAVLGHEIVHAAARHGAKSMERGVFLQGAMLAVGLATRD
                     SNYSNLVVGGAQLGGQLVSSKYGRDAESESDHYGMKYMKLAGYDPAAAVTLQETFVRL
                     SEGKRQDFLSGLFASHPPSQERVDLNKKTLQELGAGGEMGKEVYAQKVAKLMATREAY
                     KAYDEGKGALQKGDTEKARTLAKKAIGIEPREARFQELLGDIALTKKNTTEALAFYDK
                     AIQMQPDYFKPYVQSGIALFNDGKKEQAEPFLKKANALLPTAPGYALLGQIAEGRGET
                     SLALQHYQLAASSNSEIGKEAAARAMRIDLPRNPARYLPSGPLADNTGMVFAAVQNNS
                     SVPVGRVQVRMLQYDSHGRLIDQTRSMLITGGIAPGKRGSVATGIRIQDQAALQFFRV
                     VVEGAELAN"
     gene            2957..3151
                     /locus_tag="VNZ84_01255"
     CDS             2957..3151
                     /locus_tag="VNZ84_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWU65604.1"
                     /translation="MATQDTCCSVAPYFEIHPGKAAAFRAICEKYVAWSSREPGCLYY
                     GFSFDGDLAHCREGFVDANA"
     gene            complement(3354..3860)
                     /locus_tag="VNZ84_01260"
     CDS             complement(3354..3860)
                     /locus_tag="VNZ84_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HWU65605.1"
                     /translation="MHNATQRLYSRGRGLLYTLIWLGLAALVWLLIDRVQHPNTLEQI
                     GQQKVVSLKRGPDGHYSAEALINGERVRVLVDTGATGVAISQHVANRLGLVSHDAINT
                     HTANGTSVSYLVRLKTVQLGGVVAHDVAATISPGLEGDALLGMSFLGRMDVRLHRGVM
                     TISDGETK"
     gene            complement(3878..5281)
                     /locus_tag="VNZ84_01265"
     CDS             complement(3878..5281)
                     /locus_tag="VNZ84_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWU65606.1"
                     /translation="MIALPASIFKAYDIRGIVDETLTMEVVHQIGLALGSLAEEHGEQ
                     SICVGYDGRLSSPALTAALVEGITQAGINVVNLGMVTTPMLYFAAHYLAETSSGIMLT
                     GSHNPPQYNGLKMVIADQTLSGEAIQALKLRIMQQQFCHSEHPGQQSSYDIYPAYQGA
                     IVSDIQLARPMRVAIDCGNGVAGAYAPALFTALGCEVDQLFCEVDGHFPNHHPDPVEA
                     KNLQDVIASLATGEAELGLAFDGDGDRLGVVTKQGQIIRSDRQLMLFVEDVLMQQPGS
                     HVVFDVKSTRHLSPWIREHGGKPVIWKTGHSLMKAKIKETGAAMGGELSGHLFFNDTK
                     AGKPRWFGFDDGLYSAARLLEIVSRSPNASAVLEALPDSVNTPELQIAMQEGEPHALI
                     TTLQHAAQFPDAIEIITVDGLRVEYTDGFGLIRASNTTPVLTLRFEAETTSALARIQQ
                     AFRQVLGNTRPDLKIPF"
     gene            complement(5282..5476)
                     /gene="iscX"
                     /locus_tag="VNZ84_01270"
     CDS             complement(5282..5476)
                     /gene="iscX"
                     /locus_tag="VNZ84_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HWU65607.1"
                     /translation="MKWTDSQRIAEELYDKHPEIDPKTIRFTDLMQWVMDLEDFDDEA
                     SRCGEKILEAIQLAWIEEAE"
     gene            5633..7645
                     /locus_tag="VNZ84_01275"
     CDS             5633..7645
                     /locus_tag="VNZ84_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWU65608.1"
                     /translation="MTTKKTASIGRWVAGLLMMIVLAVIGCEIAGWPFLRQPFQQFLQ
                     DKLHRTVRIERPFHLQLFGGLRLQVGGLKLSAPEGFDVPYFVDAQGLDLSLRYRDLRS
                     LKDGDPYRIQAIRADKLDAYLIRHAGRQATWDFQLDDTSPPRPFPLIEQLAVNEGQAK
                     VADAVTDAQLTVKFFTEEGAHLVTPESSVKIEGIFRRLPLQGELVTQGFLPVATQGKD
                     SPPINSHGRLKYGAVNAQFNGAVYDLFGTQRVKGKVDVTGPSLADVGDLLNLTFPRTS
                     SFKVVAEVEKGVHEWKVRIPTAKIGQSRLSGQFVYDLAPEIPMLSGKLEGTLLVLADL
                     APAFGAAQGNPESKAHPGKLFPDESLDFTTYGRMNADIDVHLDAVDLGSAFRQKISPL
                     KLHLTLIKHKLSLADLEASTAQGSLSGALSIDAHDASNPDLSPPAAPDWDIQLAVRDI
                     RLEQWLKVQPKPSQPGKEKAADSPPAYITGQLTGKAKLQGRGRSTAALLKSLNGQLAL
                     IVRKGEISHLIVEAAGLDIAQAVGVLIKGDQPLPMQCAAMGWQAKQGVLTPDAALIDT
                     PVTTVTVVGDINLGQEQLNLKLQASPKNFSPFTVRSPILVTGPFVDPKVSLSPGPIAA
                     RVAGGVLLALINPFAAIVPFLDPGSKAEAADEGCQHTLQQLKARKH"
BASE COUNT         1809 a         1936 c         2197 g         1901 t
ORIGIN      
        1 tccgtaaata ccagaccttt ggcggcatca ttttgtctgc cagccacaac ccggcaggcc
       61 ccgatgatga ctttggcatt aaatacaaca ccggtaatgg cggaccggca cccgaaaaaa
      121 tcacggatgc catttttgcc aacagcaagc agattaccca atacaagctg cttgatttac
      181 cggatgttga tttgtccagt atcggtgtca ccacgctcga tggttttgaa gttgaggtga
      241 ttgattcggt gagtgattac gcggacttaa tgcaaagcat gtttgatttt tctgctatca
      301 agcgctggct agcagccggg catcgcctgc agtttgatgc catgcatgcc gtgaccgggc
      361 cttatgccaa agagattttt gttaaccgct tgggcgcacc cgccagcagt ctgatgaact
      421 gcgaagtgtc tgaaaatttt ggtggtggtc atcctgatcc taacctgact tatgctgaag
      481 atctggtcaa aatcgcttac ggcccgcagg caccagattt ctcggcagcc tcagacggcg
      541 atggtgaccg taacatgatt ctgggaaatc atttcttcgt caccccttct gatagcctgg
      601 cactgattgc ggccaatgcc aaactgattc cggcatatgc ccagggtttg tccggtgtgg
      661 cgcgctccat gccaaccagt ggcgcggtgg accgtgtggc ggaaaaactg gggattgatt
      721 gctacgaaac gcctactggc tggaagttct ttggcaacct gatggatgct ggacgtgtga
      781 cgttgtgtgg tgaagaaagc tttggcactg gcagtgacca tgtgcgtgag aaggatggct
      841 tatgggcagt attgtgctgg ctgaatctgc tggctgctac gggtgaaagc gtggagtctc
      901 tggtgcgcaa gcattggcaa acctatggcc gcaatgtgta ttcccgccat gattatgaag
      961 gcgtgcctac cgagcaggcg aatgcggtga tcgcgcatat caaggcgcaa tttaatagtc
     1021 tgcctgggca agtgtttggt agctacacgg tcaagacagt ggatgacttt agctatacgg
     1081 atccggttga tggctcggtc agtactggcc agggcttgcg cattctgttt gcctgcggtt
     1141 cgcgagtggt cattcgtttg tccggcaccg gcaccgaagg cgcgaccatt cgaatttatc
     1201 ttgaggcgtt tactgaagat gctgcacagc aacaactgga tgcccaggag gcgttggcag
     1261 agctgattgc tgtttcacat cagatctcgc aactgacaca actcactggc aggcagcaac
     1321 cgacggtgat tacttaagaa gaagagtggc ctggggaaac ggctcaatga ccatgataaa
     1381 aaaaacaata ctttgcccgg tgttgctgtc cgccggttta ctcgcctgtg gcgtcaaccc
     1441 ggtgaccaaa gaacgcgagt ttcaatttgt ctcgcaagac aaagaggtcc agatcggcca
     1501 gcaaaattat agccccgcac gccagtcaca aggcggagat tacacgctgg ataacgaact
     1561 gacacggtat gtgcagcaag ttggacaacg cttggcggca gtgtcagacc ggcctgacct
     1621 gccgtatgaa tttgtggtgc tgaatgattc cgtgcctaat gcctgggcga tgccgggcgg
     1681 caagatcgca tttaaccgcg gcttgttata cgaattgcat agcgaggctg agttggcggc
     1741 ggttttggga cacgaaattg tgcacgcggc ggcgcgtcac ggcgccaaaa gcatggagcg
     1801 cggtgtgttt ttgcaaggcg cgatgctggc cgttggcctg gcgacgcgtg actccaatta
     1861 ttccaacctt gtggtaggtg gcgcgcagct gggtggccag cttgtgtcca gtaagtatgg
     1921 ccgggatgcc gagtcggagt ctgaccatta cggcatgaaa tatatgaagc tggctggcta
     1981 tgacccggcg gcggcagtca cgctacagga gacttttgtg cgcttgagtg agggcaagcg
     2041 gcaggatttc ctgtccggac tgtttgccag ccatccaccg tcacaggagc gggtagacct
     2101 caacaaaaaa acgctgcaag aattgggtgc tggcggtgag atgggcaaag aggtttatgc
     2161 gcaaaaagtc gccaaactga tggcgacccg cgaggcttat aaagcctatg acgaaggcaa
     2221 aggggcattg caaaaaggcg atacggagaa agccaggact ttggctaaaa aagcgattgg
     2281 tattgagcca cgtgaagcgc gttttcagga gttgctgggt gatattgccc tgaccaagaa
     2341 gaataccact gaagccctgg ccttttatga taaggcgatc cagatgcagc cggattattt
     2401 caaaccgtat gtgcaaagcg gtattgcctt gtttaatgac ggtaaaaaag agcaggctga
     2461 gccattttta aagaaagcca atgccttgct gccaacggca ccaggctatg ctttgctcgg
     2521 gcagattgct gaaggccgtg gtgagacttc attggccttg cagcattacc aactggctgc
     2581 cagctcgaac tctgaaatcg gcaaagaagc cgctgctcgc gccatgcgca tagatttgcc
     2641 gcgtaacccg gcccgctatc taccctctgg accgctcgct gacaataccg gcatggtctt
     2701 cgccgctgtg caaaataaca gcagtgtgcc agtgggtcgc gtacaggtac gcatgctgca
     2761 atacgatagc catggacgct tgattgatca gacacgctcc atgctgatta ctggcggaat
     2821 tgcaccaggc aagcgcggtt cagttgcgac tggtatccgc atccaggacc aggccgcctt
     2881 gcagtttttc cgcgtggtgg ttgaaggtgc cgaactggcg aattaacctg aatcaataac
     2941 ccataaacgt cctcttatgg ctactcaaga tacctgctgt tcggttgccc catactttga
     3001 aatccatcca ggtaaagcgg ctgctttccg tgccatttgc gaaaaatacg tagcgtggtc
     3061 ttcgcgtgaa ccaggctgct tgtattatgg ctttagcttt gatggcgacc tcgcgcattg
     3121 ccgcgaaggc tttgtagatg cgaatgcatg aattgcacat atcgacaatt tgcgtccgat
     3181 tctgcacgaa atgggcgaag tatcccagct tgtgagaatt gaggtacatg gaccagaaca
     3241 tgagctctct aaactgagag cgcctttgtc tggcatgaat ccacagttct ttgtgctgga
     3301 atacggctat cgaaaaactt gataatactt aggtgctttt ttcgttagcg ccgttatttg
     3361 gtttctccat cactgatggt catcacacct cgatgcaacc tcacatccat tctccctaaa
     3421 aagctcatgc caagcagggc gtcaccctcc agacctggtg aaatcgtggc ggccacatca
     3481 tgcgcgacca caccgccgag ctgaactgtt ttcaggcgta ccaagtagga cacggaagtg
     3541 ccgttagctg tatgtgtatt gatggcatcg tggctgacca gccccaagcg attggcgaca
     3601 tgttgcgaga tggcgacgcc ggtggctccg gtgtctacca gcacacgcac acgttcaccg
     3661 ttaatcaggg cttcggcgct gtagtggcca tcggggccgc gtttgagact gaccactttt
     3721 tgctggccga tctgttctag tgtattgggg tgttggacac ggtctataag cagccagacc
     3781 agtgcggcga ggcctagcca gataagcgtg tagagcaggc cgcgcccgcg cgagtaaagt
     3841 cgttgagtag cattgtgcat gctgattgaa tggaggctta aaaaggaatt ttcaagtctg
     3901 gtctggtatt gccaagcact tgcctgaatg cttgttgaat acgcgccaaa gctgatgtag
     3961 tctcggcctc aaaccgcaag gtaagcactg gcgttgtatt ggatgcacgg atcaggccga
     4021 agccgtcggt atattccacg cgcaatccat ccacagtgat aatttcaatc gcatcaggga
     4081 attgtgcagc gtgctgtaat gtcgtgatca gggcatgtgg ttcaccttcc tgcatggcaa
     4141 tttgcaattc aggggtgtta acgctgtctg gcaaggcttc cagcactgcg ctggcattcg
     4201 gactgcggct aacgatctct agcaagcgtg ctgcgctata cagtccgtca tcaaagccaa
     4261 accagcgtgg cttgcctgct ttggtatcgt taaaaaacag gtgaccggag agctcgccac
     4321 ccatggccgc ccccgtttcc ttgatcttgg ctttcatcag tgagtgcccg gttttccaga
     4381 tcactggttt tccaccatgc tcgcgtatcc atggcgataa atggcgtgtc gatttcacat
     4441 cgaataccac atgactgcca ggttgctgca tgagcacatc ttccacaaac agcatcagct
     4501 ggcggtcgct gcggataatc tgtccttgct tggtgacaac ccctaaacgg tcgccgtcac
     4561 catcaaatgc cagccccaac tcggcttcgc ctgtagccaa actggcaatc acatcttgca
     4621 ggtttttggc ttcgaccggg tcggggtgat ggttgggaaa gtggccgtcc acctcgcaaa
     4681 acagttggtc gacctcgcaa cccagggccg tgaatagtgc gggtgcataa gccccggcaa
     4741 cgccgttgcc acagtcgatg gctacacgca ttgggcgcgc gagctggata tctgaaacaa
     4801 tggctccctg gtaggcaggg taaatatcgt aagaggattg ttggcctgga tgttcggaat
     4861 ggcagaactg ctgttgcatg atccgcagtt tgagtgcctg aatcgcttcg ccactgagtg
     4921 tttggtcagc aatcaccatc ttgaggccgt tgtactgtgg cggattatga ctaccagtga
     4981 gcatgatgcc actgctggtt tcagccaaat aatgggctgc gaaatagagc atgggggtgg
     5041 tgaccatgcc caggttaacc acattgatcc ccgcctgcgt gatcccttcg acgagcgcgg
     5101 cagtcagcgc cgggctggat aaacggccat catatccgac acaaatgctt tgttcgccat
     5161 gctcttctgc caaggagccc agcgctaggc caatctggtg cacgacctcc atcgtcaatg
     5221 tctcgtccac gatgccgcgg atatcgtagg ctttgaaaat ggacgctggc agtgcaatca
     5281 tttattcagc ttcttctatc caggccagtt gaatggcctc gaggattttt tcaccgcaac
     5341 gagaagcttc gtcatcaaag tcctctaaat ccatcaccca ttgcatcagg tcagtaaagc
     5401 ggatggtttt aggatcaatc tcagggtgtt tatcgtacag ttcctcggca atccgttggc
     5461 tgtcggtcca tttcattgtc gttactccat ggttaaagca tattgatgat taccattata
     5521 actgactgcc agcaccggat ttgacagaat ccacctacaa gcatgtcaga taagggggac
     5581 tggtcaggca acctgctgct cggcatgata ttagggtatg gatgataatg aaatgaccac
     5641 aaaaaaaacg gcaagcatcg gcagatgggt tgccggtctc ttgatgatga tagtgttggc
     5701 cgtgataggc tgcgagatag ccggctggcc gttcctgcgt caaccctttc aacagtttct
     5761 acaagataag ttacaccgta cggttcgaat tgagcgaccg ttccacttgc aactgtttgg
     5821 tggccttcgc ctgcaagtgg gtgggcttaa actttcggcc ccagagggat ttgatgtccc
     5881 ttattttgtc gatgcacagg gattggatct ttcgttgcgc taccgggacc tgcggtcgtt
     5941 gaaggacggc gatccttacc gtatccaggc aattcgtgca gacaagcttg atgcttacct
     6001 gatccggcat gcaggcaggc aggcgacctg ggattttcaa ctggatgata cgagtccgcc
     6061 tcggcctttc cccttgattg aacaacttgc tgtcaatgaa ggacaagcca aggtggctga
     6121 tgctgtcacc gatgcccaat tgacagtgaa gttttttacc gaagaggggg cgcatttggt
     6181 gacgcctgaa tcgagtgtaa aaattgaggg aatatttcgg cgtttgccat tgcaaggtga
     6241 actggtcacg caaggttttt tgcctgtagc gacacagggc aaagactctc caccgattaa
     6301 ctcccacggc aggctcaaat atggggcagt caacgcacag tttaatgggg cggtatatga
     6361 cttgtttggc acgcaacgcg tcaaaggtaa agtagatgtg acaggacctt cactggctga
     6421 cgtgggggac ttgcttaacc tgacttttcc acgcacctct tcttttaaag tcgtggccga
     6481 agttgaaaaa ggtgttcatg agtggaaggt acgtatccca accgcaaaaa tagggcagag
     6541 ccggttatcc ggccagtttg tttatgatct tgcgcctgaa attcctatgc tgagtggcaa
     6601 gctggagggc acgctactgg tgttggctga cctggcgcca gcgtttggtg cggcgcaagg
     6661 caacccggag agtaaagcgc accctggcaa gctgtttccg gatgaatcgc tggactttac
     6721 gacctatggc cgcatgaatg cagacattga tgtgcatttg gatgcagtgg atttgggcag
     6781 tgcattcagg cagaagattt ccccgttgaa gttgcattta acgctgatta aacacaagct
     6841 aagcctggcc gaccttgaag ccagtacggc gcaaggtagc ctgtcagggg cattgagtat
     6901 tgatgcccat gacgccagta atccggattt atcgccgcca gcggcgccgg attgggatat
     6961 ccagcttgcg gtacgggata ttcgccttga acagtggctt aaggtacagc cgaaaccctc
     7021 gcaaccgggt aaggaaaagg cagcagatag ccccccagcc tatatcaccg gtcaattaac
     7081 cggcaaggca aaactgcaag gccgtggccg ttcaacggcg gcattgctta aatccctcaa
     7141 tgggcaactg gcattgatcg tgcgcaaagg tgaaatttcc catttgatcg tagaggcggc
     7201 cggtttggat attgcacaag ctgtgggcgt gctgattaaa ggtgaccaac ccttgcctat
     7261 gcaatgtgct gccatgggct ggcaagccaa acagggggtg ttgacgccag acgcggcctt
     7321 gattgatacc ccggtaacga cggtgacggt ggtgggtgac attaacctgg ggcaggagca
     7381 gctcaatctt aaactgcaag ccagtccgaa aaacttttcc ccttttaccg tacgttcgcc
     7441 aatactggtc accgggccat ttgttgatcc caaggtttca ttgagcccag gcccgattgc
     7501 agcacgcgtg gcagggggcg tgttactggc gttgattaat ccttttgcag cgattgtgcc
     7561 tttcctggat cctggcagta aagcggaagc ggccgatgag gggtgccagc ataccttgca
     7621 gcaattgaaa gcccgcaaac attagcttct tggtttgggc aaaaaagaac ccaaccgaag
     7681 ttgggtttag ggagcgattt tggagatata cgcttacggt tactactaag cataagccgt
     7741 gccaggattg taaaattcat taaaatcaga tggataggtg tttttcgttg ttttgtgggc
     7801 tgctgccagt tccgcatttt atgcacctgt ttggtgcatg tgg
//
LOCUS       DATMSK010000039         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_285745,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000039 DATMSK010000000
VERSION     DATMSK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285745"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /gene="mreD"
                     /locus_tag="VNZ84_01280"
     CDS             <1..278
                     /gene="mreD"
                     /locus_tag="VNZ84_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWU65609.1"
                     /translation="GSLLGQHALTFAITAFLGMIYQRRLVLFNRWQLYLYVIFLFGVQ
                     RILILLLKLFAGGEIPDWTYGLPVISDLILWQFMIIWFGELTRPKKL"
     gene            283..2214
                     /gene="mrdA"
                     /locus_tag="VNZ84_01285"
     CDS             283..2214
                     /gene="mrdA"
                     /locus_tag="VNZ84_01285"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWU65610.1"
                     /translation="MGYSTELKNHQNEQHKFRVRLVFLVLLVLGAFSALLGRFYFLQV
                     YRYDYYQTLAENNRISIVPITPNRGLISDRNGIILAHNFFVYTLEITPSKTDDLQDTI
                     ADLGKLVEISASDLKRFKKLRAQSHTFESIPIRTHLNEYEAAKFAVNRFRFPGVELKS
                     RLFRHYPYGTLGTHGIGYISRINEHDLKQLKENNQLSNYKGTEHIGKAGVEQAYEADL
                     HGITGFQQVEIDADGRAVRVLSSASPTSGNNIVLSMDAKLQEIVEHEFAGRRGALVAI
                     QPKTGEVLAMVSMPSFDANLFVDGIDYDNWNNLNNSLDKPLINRALKGIYPPGSTFKP
                     FVALAGLESGKRQPPFTISDPGYFTLPNSRHRYRDWKPSGHGAVDMRKAITVSCDTFF
                     YGLAVELGIERLTDFVRHFGFGEKTNIDIQGELSGLLPTPTWKQKRYKQPWYMGETVI
                     VGIGQGYTLVTPMQLAYATATLANSGKAIRPHLVNAIVDAKTNQVKMLPHAIADQLNL
                     NPENLEIVKLGMMDVTRPGGTAAAVGANAGYDIAAKTGTAQVVGIKQNEKYNEKLIAE
                     RHRDHAWFIAYAPAEDPKIAIAVIVENGSHGGSTAGPIARKAMDYYLLGKVPVDPTAA
                     PAPETTVTNAPEVEEEPHD"
     gene            2207..3304
                     /gene="rodA"
                     /locus_tag="VNZ84_01290"
     CDS             2207..3304
                     /gene="rodA"
                     /locus_tag="VNZ84_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWU65611.1"
                     /translation="MIRQWIGHFIRHIDSILLATVLMVLVVALMVLYSASGQSLLKVN
                     AQLINIVVMMGVMLLVANTQPQHIERIALPAYLLGLALLIAVALIGDISHGARRWLNL
                     GVTKIQPSELMKLAVPIMLSWYFSKRESMLRTADYVVAGVILLVPVLLIAKQPDLGTA
                     TLVFAAGFYVIFLAGLSWKFIFGSAIAFGAALPILWSMLHDYQRRRVEILIDPSQDPL
                     GAGYHIIQATIAIGSGGTTGKGWLNGTQSQLDFVPERTTDFIFAVFAEEFGLVGCSLL
                     LLLFTIIIGRAFIIASQAKSTFSRLLAGSIATTFFTYCFVNMGMVSGILPVVGIPLPL
                     VSYGGTSLLTLGLSMGLLMSVQSHKRLVATD"
     gene            3332..>3655
                     /locus_tag="VNZ84_01295"
     CDS             3332..>3655
                     /locus_tag="VNZ84_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWU65612.1"
                     /translation="MSFYLQRLSILLLAAWLAACNSTMPPRQSTPTTPSKPSTTERPA
                     NTAPAPITNAPFPGGAAPNGPKIINIDPKPETTGNPTDGIDPNIPLETSVTPKLEPIV
                     KGTTKP"
BASE COUNT          873 a          998 c          893 g          891 t
ORIGIN      
        1 ccggaagctt gctggggcag cacgcactta cttttgccat taccgccttc ctgggcatga
       61 tttaccagcg ccgccttgtt ctatttaacc gctggcagct ctatttgtac gtgatattcc
      121 tgtttggcgt acaacgtata ctgatcctgc tactgaaatt atttgcaggt ggggaaatcc
      181 ccgattggac ctatggtcta cccgtcattt ctgacctgat attgtggcag ttcatgatta
      241 tctggtttgg tgaactgact cgcccgaaga agttataaac acatggggta tagcaccgaa
      301 ctcaaaaacc atcaaaacga acagcacaag ttccgggttc gtctggtttt cctggtattg
      361 ctggttctcg gcgctttttc ggcattgttg gggcgctttt atttcctgca agtctaccgt
      421 tacgactact accagacgct ggcagaaaac aaccgcattt caattgtacc gatcacgcct
      481 aaccgtggcc tgatttcaga ccgtaacggc atcatcctgg cgcataactt ttttgtgtat
      541 acgcttgaaa tcacgccgtc caaaaccgat gatttgcagg acacgattgc tgacctgggc
      601 aaactggttg aaatctctgc atcagatcta aaacgtttta aaaaactacg ggcgcaaagc
      661 catacgtttg aaagcattcc gatccgtacc cacctcaacg aatatgaggc agccaagttc
      721 gccgttaacc gcttccgttt tccaggcgtt gagctcaaat cacgcttatt ccgccattat
      781 ccctatggca cactgggcac gcatggcatt ggctatatca gccgcatcaa tgaacacgac
      841 ctgaaacagc taaaagaaaa caatcagctc tctaattaca agggcaccga acatatcggc
      901 aaggctggcg tcgagcaggc ctatgaagca gatttgcatg gcatcaccgg cttccagcag
      961 gtcgaaattg atgcagatgg tcgtgccgta cgcgtactct ctagtgcatc ccccacttca
     1021 ggcaacaaca ttgtgttgtc gatggatgcc aaattgcaag aaatcgtgga gcatgaattt
     1081 gcgggccgcc gtggtgcgct ggtggcaata caacccaaaa ccggcgaagt gctggcaatg
     1141 gtcagcatgc cttcattcga tgctaacctg tttgtcgatg gtattgatta tgacaactgg
     1201 aataacctga acaactccct ggacaaacca ctgattaacc gcgcactcaa agggatttat
     1261 cctccaggct caacattcaa gccatttgtg gcattggctg gtctggagtc tggcaaacgc
     1321 cagcctccct tcaccatcag cgaccccggt tacttcacct tgcccaacag ccgccatcgt
     1381 taccgcgatt ggaagccttc agggcatggt gccgtcgaca tgcgaaaagc gatcaccgtt
     1441 tcttgtgata cgttttttta cggcctggcc gttgaactcg gcattgaacg cctgacagat
     1501 tttgtccgcc attttggctt tggtgaaaaa accaacattg atattcaagg tgaactctcg
     1561 ggcttgctgc ctaccccgac ctggaagcaa aagcgttaca aacaaccgtg gtacatgggt
     1621 gaaaccgtga ttgttggtat tggtcagggt tacacgctgg tcacgcccat gcagcttgcc
     1681 tacgccaccg ccacactggc caatagtggt aaagcgatcc ggccacattt ggtgaacgcc
     1741 attgtggatg ccaaaacgaa ccaggtaaaa atgctgccac acgcaattgc cgaccagctc
     1801 aacctgaacc cagaaaatct ggaaatcgtg aaactgggca tgatggatgt gacgcgcccg
     1861 ggaggcacgg ctgctgccgt tggcgccaat gccggctatg acattgcggc caaaaccggg
     1921 acggcgcagg tggtcggtat caagcaaaac gaaaaataca acgaaaagct gattgctgaa
     1981 cgccatcgcg accacgcctg gtttattgcc tacgcgcccg cagaagatcc gaaaattgcc
     2041 atcgcggtga ttgtagaaaa cggtagccat ggcggctcaa cggcaggtcc gattgcgcgc
     2101 aaggccatgg actattacct gctggggaaa gtgcctgttg atccaactgc tgcgccagca
     2161 cccgaaacca cagtcactaa cgcgcctgaa gtcgaggagg agcctcatga ttagacaatg
     2221 gataggccac tttatccggc atatcgattc catcctgctg gctactgtac tcatggtgct
     2281 ggtagtcgcc ctcatggtgc tttacagtgc gtccggacag tcgctgctta aggtgaatgc
     2341 gcagttgatc aatattgtcg tgatgatggg ggttatgttg ttagtagcga acacacagcc
     2401 acagcacata gaacgtatcg cgctacctgc ctacttgtta ggcctcgcct tgttgattgc
     2461 ggtagcgctg atcggtgata tcagccatgg cgcacgccgt tggctgaacc ttggcgtcac
     2521 caaaatacaa ccttctgaac tcatgaaact agccgtaccc atcatgcttt cctggtactt
     2581 ttccaaacgt gaaagcatgt tgcgcacagc cgactatgtg gtggctggcg taattctgtt
     2641 ggtgccagta ttgctgattg ccaaacagcc cgacctgggc acggccactt tggtatttgc
     2701 cgccggtttt tatgtgattt tcctggcagg actgagctgg aagtttattt tcggtagcgc
     2761 gatcgccttt ggtgccgcgt tgccaatcct gtggtccatg ctgcatgact accagcgccg
     2821 ccgggtagaa atcctgattg acccaagcca ggatccactt ggcgctggtt accatattat
     2881 ccaggccacc attgccatcg gttctggcgg caccaccggc aaaggctggc tcaacggcac
     2941 gcaatcacaa ctggactttg tacccgagcg taccactgac ttcatttttg cagtgttcgc
     3001 tgaagagttt ggcttggtag gctgcagctt gttactccta ctattcacca ttattattgg
     3061 tcgtgccttc atcatcgcat cacaagccaa gagtactttt tcacgcttgc tagctggcag
     3121 tatcgccacc acctttttta cctactgttt tgtcaatatg gggatggtga gtggcatctt
     3181 gcccgtggtt ggcattcctt taccgctggt cagctatggt ggcacttcat tgctgacact
     3241 ggggctgagc atgggcttgc tgatgagtgt ccagtcacat aaacgcctgg ttgccacgga
     3301 ttagccacca aaaataccaa tagggataaa gatgtctttt tacttgcaac ggttgagtat
     3361 cctgctgctg gcagcttggc tggcagcctg taacagcacc atgccccctc gccaaagcac
     3421 acccacaacc cccagcaagc cttccaccac agagcgtccg gccaacactg caccggcacc
     3481 catcacgaat gctccttttc caggaggcgc tgcaccgaat ggccccaaaa tcattaatat
     3541 tgatcctaag ccggaaacca cgggaaaccc caccgatggc atagatccga atatcccgct
     3601 ggaaaccagc gtcaccccca agttggaacc gattgtgaaa ggcactacca agcct
//
LOCUS       DATMSK010000040         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_238787,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000040 DATMSK010000000
VERSION     DATMSK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238787"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VNZ84_01300"
     CDS             complement(<1..594)
                     /locus_tag="VNZ84_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU65613.1"
                     /translation="MAFDEKLLNGISVLSAIIDSGSFAGAGEILDMSQSGVSRAVARL
                     EARLGVRMFDRTTRSVSLTDEGRRFYEQILPLLTKMEEVTTSVATDKVEVRGRLRVTV
                     DPFFSRLVLGPQLGAFIAQYPGLHLEIMTRHQLGDMVADGFDLALRFGHPRTSSLIAS
                     KLFDTNILTVASPAYIQRYGRPKTPQDLVTHACIQFRD"
     gene            709..1575
                     /locus_tag="VNZ84_01305"
     CDS             709..1575
                     /locus_tag="VNZ84_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HWU65614.1"
                     /translation="MFAITGITGQVGGEVARNLLANEKQIRAVVRNADKAKHWEKPES
                     HIFIADMNDATALTKAFTGTEGVFIMLPPTFDPSEGFTEARQTIEAIREALARANPPK
                     VVCLSTIGADSNNPNLLNQLGMLEHELSTLDTPIAFLRAAWFMENSNWDIASALEKGV
                     IYSLLQPLDKPFPMIATADVGQTAAELLQEDWQGIRIINLETEVRISPNDIAAAFTKT
                     LGKTVNAESVPQDIWLDLFTSQGMKNPLPRIQMLNGFNQGWIEFSPHGTESRKGKVKM
                     ERVLEKLISNAT"
     gene            complement(1688..4294)
                     /gene="pepN"
                     /locus_tag="VNZ84_01310"
     CDS             complement(1688..4294)
                     /gene="pepN"
                     /locus_tag="VNZ84_01310"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWU65615.1"
                     /translation="MRTENAVAPKTIYLKDYTPALYLAQQVNLSFNLLPGKTIVTSEV
                     KYVKNPAGVSSDLVLDGEDQTIVSVALNGQPFAGYTVADGQMTITNPGNEFTLTIVSE
                     INPEANTALEGLYQSQGNYCTQCEAEGFRRITYYQDRPDVLSVFTVRIEADAKLYPVL
                     LSNGNLQEEGGLPDGRHFRVWHDPFPKPSYLFALVAGDLQRVADTFTTMSGRKVDLHI
                     YVRAGDEKQCDHAMESLKKSMKWDEDTYGREYQLDLFNIVAVSDFNMGAMENTSLNIF
                     NTALVLAHQETATDSDFMSVEGVIGHEYFHNWTGNRVTCRDWFQLSLKEGFTVFRDQE
                     FSSDMNSRAVQRIDDVNGLRRLQFPEDGSPLAHSVQPDQFIEISNFYTKTIYEKGAEL
                     IRMQHTLLGAETFRKATDLYFKRYDGHAVTCDDFVQCMADASGRDLSQFFRWYKQAGT
                     PTLQVSSQYDAAQQSYTLAFKQSQPDTPEQTDKQPLHIPVAIGLLDANGKEILPTRVL
                     EVTAREQTFTFTGITAKPVPSILRGFSAPVKLETDLSDDDLRLLQVHDTDGFNKWEAG
                     QTLALRTIQRVMADPQADISQFVADVGTLIAQGLSGQGDKALLAHALSLPMIGVIGQY
                     LPVIDPAAIDAARSSILTQIKRAHKDTLAALYDANGNPGEFSITTEAMGRRALQAAVL
                     EILTSTNGTGCAARAKAHYDAADNMTDRVTALASLSDSSQPQRAEVLADFYNRFKGYP
                     LVVDKWFSLQAIANRAQIFDDLAMLRQHPDFNIKNPNRVRSLYSAFAINNPVKFHDPS
                     GKGYAFLRDAIIELNTINPQIAARLVTPLREWKRYTPALQAQMKAALEAILATPDLSG
                     DVFEVVSKSLNG"
     gene            4464..4748
                     /locus_tag="VNZ84_01315"
     CDS             4464..4748
                     /locus_tag="VNZ84_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HWU65616.1"
                     /translation="MKFGFTQLKSLSNLQKHGISLEDAKLLDWSLLVFIEDERKDYGE
                     VRFIGYAPIENRLYCIVYTERNEVIHVISLRKANSREVIRYVKARQNIQD"
     gene            4717..4995
                     /locus_tag="VNZ84_01320"
     CDS             4717..4995
                     /locus_tag="VNZ84_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HWU65617.1"
                     /translation="MSKHAKTFKIKNLELELPDADEEARINAGIEADPDTFEVSDRQF
                     KKMRGRPVGSNKTLVHIRLDNDVVEKFKATGSGWQTRINEALKKANPV"
BASE COUNT         1384 a         1264 c         1155 g         1242 t
ORIGIN      
        1 atccctgaat tgaatgcagg cgtgagtaac caaatcttgc ggtgttttcg gtcgcccata
       61 gcgctgtatg taagcgggtg aagcaacggt aaggatgttg gtatcaaaca acttactggc
      121 aattaagcta gatgtacggg gatgaccaaa gcgtaacgcc agatcaaaac catcagccac
      181 catgtcaccc agttgatgtc ttgtcatgat ttccagatgt aatccggggt actgcgcaat
      241 aaatgcacct agttgcgggc ccaatacaag tctcgagaaa aaaggatcga cagtgacgcg
      301 caaacgccct cgcacttcga ctttgtctgt agccacggat gttgtgacct cttccatttt
      361 tgtcaataac ggcagtattt gctcgtaaaa tcggcgccct tcatcagtca aactgacaga
      421 gcgcgtggtg cgatcaaaca tacgcacgcc taaacgagct tccagtcttg ccacagctct
      481 gctgacgcca gattgcgaca tatccaatat ctcaccagca cccgcaaaag agccgctatc
      541 aataatggct gacaggacac ttataccgtt gagcagtttc tcatcaaacg ccacaattga
      601 tgcctttttg tcatatatga aattacattc atgctatcgc ataactcagt taaaaagtag
      661 agaattttgc tcatggcgac aaacgtcgca gttattaagg agaaggttat gttcgcgatt
      721 actggaatta ctggacaagt tggtggggaa gtagccagaa acttattggc aaacgaaaaa
      781 caaatacgcg cggttgtgcg taatgctgat aaggcaaagc actgggaaaa gccagagagc
      841 cacattttca ttgctgatat gaatgatgct actgcactaa ccaaagcatt tactggcact
      901 gaaggcgttt tcattatgct ccccccaaca tttgaccctt cggaggggtt tactgaagcc
      961 agacagacaa tcgaagccat ccgtgaagcg cttgcaagag ccaacccacc aaaagttgta
     1021 tgtttatcga ccataggcgc cgacagtaac aacccaaacc tgcttaatca gcttggtatg
     1081 cttgaacatg agctaagcac actcgacacg ccaatcgcat tcttacgtgc ggcctggttt
     1141 atggaaaact caaactggga tattgcatcc gctcttgaga agggggtgat ctattcctta
     1201 ctgcaaccac tggataaacc tttccccatg attgccaccg ccgatgtagg tcagactgca
     1261 gcagaattgc tacaagaaga ttggcaaggt attcgcatca tcaatctaga aacggaagtt
     1321 cgtataagcc ccaatgatat cgcggccgcc tttacgaaaa cgctaggcaa aacagtcaat
     1381 gctgaatctg tgccccagga tatctggtta gatttgttta cctctcaagg aatgaagaat
     1441 cctctgcctc gaattcaaat gctcaatgga tttaaccaag gttggattga gtttagccca
     1501 cacggaacag aaagcaggaa aggtaaagta aaaatggagc gcgtgttgga gaaactgata
     1561 tccaacgcaa cctagtaaga tgacttgtaa ttcagttagg ctaaatttct agtagatata
     1621 gatcaatcaa ctcgaaaacg aaggcgccgc tttagagacg ccttcatcac atacaactac
     1681 agcccaatta tccgttgagg ctcttgctca ccacctcaaa cacatcgcct gacaaatcag
     1741 gcgttgccag aatcgcctcc agcgccgctt tcatctgcgc ttgcaaagcg ggtgtgtagc
     1801 gcttccactc acgcaatggt gtcaccaacc gcgcggcaat ctgcgggtta atggtgttga
     1861 gctcaataat cgcatcacgc aagaaagcat aacctttgcc acttgggtca tggaatttga
     1921 ccggattgtt aatcgcaaac gcgctataga gcgagcgcac acggttcggg ttcttgatat
     1981 taaagtccgg atgttgacgc aacatggcca ggtcatcaaa aatctgtgca cggttggcaa
     2041 tcgcttgcag tgagaaccac ttatcgacta ccagcggata gcctttaaac cggttgtaga
     2101 aatctgccaa gacttccgca cgttgtggct ggctactatc tgacaaactc gccagcgctg
     2161 tgactctgtc cgtcatatta tccgcagcat cgtaatgtgc cttagcgcgt gccgcacagc
     2221 cggtaccatt ggtggaggtt aagatttcca gcacggcggc ttgcagcgct ctacgtccca
     2281 tcgcctcggt ggtgatggag aactcgcccg gattgccatt cgcgtcgtat agcgccgcta
     2341 atgtatcttt atgcgcgcgt ttaatctgcg tcaggatgct gctacgtgcc gcatcaatcg
     2401 cagcggggtc gatcaccggc aaatactgcc caatcacgcc aatcatcggc aaactcagcg
     2461 cgtgcgccag caaagcttta tcgccctgcc cgctcaagcc ttgtgcgatt aatgtaccca
     2521 catcggccac aaactggctg atatccgctt gtggatccgc catcacacgc tgaatagtac
     2581 gcaatgccag tgtctggcca gcttcccatt tgttaaagcc atcagtgtca tgcacttgca
     2641 gcaagcgcaa atcatcgtca cttaaatccg tttccagctt cactggcgcg gagaaaccac
     2701 gcagaattga agggacaggt ttggcagtaa ttccagtgaa agtgaatgtt tgctcacgtg
     2761 cagtcacttc cagcacgcgg gtcggcagaa tctctttacc attcgcgtcc aacaaaccaa
     2821 tggcgaccgg aatatgcaac ggttgcttgt ccgtttgctc tggcgtatct ggttgtgatt
     2881 gcttgaatgc cagcgtgtag ctttgctgtg ctgcatcgta ttggctactg acttgcaatg
     2941 tcggcgtgcc tgcttgttta taccagcgga agaactgcga caaatcgcga cctgacgcgt
     3001 ctgccatgca ttgcacaaaa tcgtcgcagg tcacagcgtg gccgtcatag cgcttaaagt
     3061 ataaatccgt cgctttgcgg aaagtctcag cgcccagcag cgtgtgctgc atgcggatca
     3121 actccgcacc tttttcataa atggtcttgg tgtaaaagtt gctgatctca ataaactgat
     3181 ctggctgcac cgagtgcgct aatgggctgc catcttcagg aaactgcaag cggcgtaagc
     3241 catttacatc gtcaatacgc tgtaccgcac gcgagttcat gtccgagctg aactcctgat
     3301 cacggaagac ggtaaagcct tctttgagtg atagctggaa ccagtcacgg caggtcacac
     3361 ggttaccggt ccagttgtgg aaatactcgt gaccaatcac gccttccacg ctcataaaat
     3421 cgctgtcggt cgcggtttcc tgatgcgcca gcaccagcgc cgtattgaaa atattgagcg
     3481 aggtgttttc catcgcgccc atattaaaat cgctcaccgc aacgatattg aacaagtcga
     3541 gctggtactc gcggccataa gtgtcttcat cccacttcat cgactttttc agtgactcca
     3601 tcgcatggtc gcactgcttt tcatcgcccg cccgcacata aatatgcaaa tccaccttgc
     3661 ggccagacat ggtcgtaaac gtatccgcca cgcgctgcaa atcgcccgcc accagcgcaa
     3721 acaaataact aggtttaggg aaaggatcat gccacacacg gaaatgacgg ccatccggca
     3781 aaccgccctc ttcctgcaaa ttaccgtttg agagcagcac cggatacaac ttggcatccg
     3841 cctcaatgcg tacggtaaac acactcaata catcagggcg atcttggtag taagtaatcc
     3901 gcctaaagcc ctctgcctca cactgcgtgc agtagttacc ctgtgattga tacaggcctt
     3961 ccagcgcagt attcgcttca gggttaatct cgctgacgat agttaacgtg aattcattgc
     4021 ctggattcgt aatcgtcatc tgcccatcag ccacggtata gccagcaaac ggctgaccat
     4081 tcaaagcaac cgaaacaatg gtttgatctt caccatccag caccaagtcg ctcgacacac
     4141 ccgccgggtt tttcacgtac ttcacttcag acgtgacaat ggttttaccc ggcaacaggt
     4201 taaaactcaa attcacctgc tgcgccagat aaagcgctgg agtgtagtct ttgaggtaga
     4261 tggtttttgg tgcaacagcg ttttcggttc gcatgaattt acctgagagt ttttagagtt
     4321 aagtgaggat tatatttgag agtagtactt atggaattgg tttagtacgc cgcttaacaa
     4381 aatgttttat acaacattaa ttaacatatg ttgtgttcat gcaaacccaa cattgactaa
     4441 taatttattg taattacaat aatatgaaat tcggcttcac ccaactcaaa agtttatcaa
     4501 acctccaaaa gcatggcata tcgctagaag atgcaaaatt acttgattgg agcttgctgg
     4561 tatttattga agacgaacga aaagactatg gcgaagttcg ttttataggt tacgccccta
     4621 tagagaatcg tctgtattgc atcgtttata cagaacgcaa tgaagtgatt catgtaatta
     4681 gtttgcgtaa agcaaatagc cgcgaggtaa taagatatgt caaagcacgc caaaacattc
     4741 aagattaaaa acctggaact tgagctgcca gatgcggatg aagaagcacg catcaacgcc
     4801 gggattgaag ccgatccaga tacatttgaa gtaagtgata ggcaatttaa aaaaatgcgc
     4861 ggcaggccag ttggcagcaa caaaacacta gtgcatatcc ggctagataa tgatgtggtg
     4921 gaaaaattta aggctacggg ttcaggctgg cagactcgga ttaacgaagc tttaaagaaa
     4981 gcgaatccag tttaattcag ccccaaaatt atctgcttct tggcaacttc catctcatcc
     5041 acaac
//
LOCUS       DATMSK010000041         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_121482,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000041 DATMSK010000000
VERSION     DATMSK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121482"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /gene="rlmJ"
                     /locus_tag="VNZ84_01325"
     CDS             <1..549
                     /gene="rlmJ"
                     /locus_tag="VNZ84_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HWU65618.1"
                     /translation="GSPCIASHFLNAADKLRLFELHPADAALLEQHFAPQKRQVSVLA
                     KDGFEGIKACLPPPTKRGIVLIDPPYEVKDDYSRVVNCIKDSLKRFATGTYLIWYPRL
                     QRPEPQQMIDRLRSLDTDYLHVTLDVQEPAADGFGMYGSGMWVINPPWTLRQAVEPHL
                     GWLAKTLAQDNTAHTTCEGRQR"
     gene            complement(546..1661)
                     /locus_tag="VNZ84_01330"
     CDS             complement(546..1661)
                     /locus_tag="VNZ84_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HWU65619.1"
                     /translation="MTLSENPTPTRLSRWLARSRMVLPLSFFIGGFVWDAITIGQKVA
                     MSDMMIFALYLIVAALLMHQLAEPYSSVRRLGERLLRWQWLQARLSSWTVQDWPYWIL
                     QFLFGSLLSALFILYFKSSSYGLAWVVTLVLGALLVANEFMESEYRRFSLCWSMFGLC
                     SILWCNFAFPFLFGSVHAIWFYLSTLLGATVTYVLYQRAPHHAGRIWPVWLIAGLLML
                     AYRADMIPPVPLVKQAVVVAYELEKTPEGYWMTVEKSPWWQFWRIDSDHFYLQPGQKL
                     VCFSAIFAPQGLQTKLVHDWQKKIKGEWVSVSTPGFALTGGRDNGYRGYTYKSNVTAG
                     EWRVRIQTSTRQTIAVNAFHVSLRSDHDSQRRRRIRY"
     gene            1882..>3592
                     /locus_tag="VNZ84_01335"
     CDS             1882..>3592
                     /locus_tag="VNZ84_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU65620.1"
                     /translation="MRDLVRQFKQKVTELKSVSNETEQALIRVVFVSFITLYLLLNHS
                     SYEPVLLCLIYLCFGFLMLFNILKDPKKNEKRQWTAMIMDITATSLEQMISGSMAGVF
                     IGVYLWLIIGYGLRYGTKFFKGCYVFSLIGFGITLYLNPYWVEHQHLAYGFFLTLLLI
                     PPHTLRLQISLEKATKEAGQANEAKTNFVSNISHEMRTPLNGIIGASELLAQTKLDSK
                     QSELLKMVGTSASSLKKLINDVLDISKIEKGKVELEDITFFLPDLIQRLQLMFQLEVE
                     RKQLWLRFDLAPETERHYVGSLHHIEQVLVNLIANAVKFTQHGGVDVMVTDTNQLTFR
                     IRDTGIGIKSEVLPLIFDSFTQADSSITRRYGGTGLGTSIAKQLVEVMGGNIDVSSQV
                     NIGTTFVVALPLMHAAAPAQPVADEINLDNQSNVVPLSSHTKFRKKIRVLIADDNVVN
                     RLILNETLQKMHCIVKAVDNGDAALDALEHHQFDLMILDYNMPEMNGLEVFNIYHALP
                     GSLPLRTVILTADATKTTQERCLRAGVYQVLTKPVVSRQIQTLIEAIASTIETGQAAQ
                     AAPG"
BASE COUNT          982 a          904 c          856 g          850 t
ORIGIN      
        1 ggctcaccct gtattgccag ccacttttta aatgccgcag ataaattgcg tttgtttgaa
       61 cttcacccgg cagatgccgc actgctggag caacattttg cgccgcaaaa gagacaggtc
      121 tcagtcctgg ccaaagatgg ctttgaaggg attaaagctt gtttaccgcc gcccaccaag
      181 cgcggcattg tcttgattga tccaccttat gaagtgaagg acgattacag ccgtgtggtc
      241 aattgcatca aggacagcct gaagcgcttt gcgaccggca catacttgat ctggtaccct
      301 cgcctgcaac ggcctgaacc gcaacaaatg atagacaggc tgcgtagcct ggataccgac
      361 tatttgcatg tcacgctgga tgtgcaggaa cctgctgccg atggctttgg catgtatggc
      421 agcggcatgt gggtgattaa cccgccatgg accttacgac aggctgtaga accccatctg
      481 ggctggttgg cgaagaccct tgcgcaagat aacactgcgc atacgacctg tgaaggacgg
      541 caacgttaat aacggatacg tcttctgcgc tgtgaatcgt ggtcgctacg cagagagacg
      601 tggaaggcgt tgacagcaat cgtttggcgc gttgaagttt gtatgcgcac gcgccattcg
      661 ccggccgtca cgttgctttt gtaggtatac ccgcgatagc cgttatctcg cccaccggtc
      721 aaagcaaacc caggcgtaga aacactgacc cactcccctt tgattttttt ctgccagtca
      781 tgaaccagct tagtctgcaa gccttgcggc gcaaatatcg ccgaaaagca gactaacttt
      841 tgccccggtt gcaagtaaaa atgatcgcta tcaatccgcc agaattgcca ccagggagat
      901 ttttcaaccg tcatccagta gccttcaggt gttttctcca gctcataagc gacgactact
      961 gcctgcttga ccaagggcac cggtggaatc atgtctgcac ggtaagccag catcaacaat
     1021 ccggcaatca gccataccgg ccagatgcgt ccggcatgat gtggtgcgcg ctggtacaac
     1081 acataggtga ccgtcgcacc gagcaaagtg ctcagataaa accagatggc gtgcacgcta
     1141 ccaaacagga aagggaaagc gaagttacac cacaaaatag agcacaagcc aaacatgctc
     1201 cagcacaaac tgaaacgccg atattcgctc tccataaatt cattagccac caggagcgca
     1261 cctaatacca acgtgacaac ccaggccaag ccataacttg aactcttgaa atacaggata
     1321 aacaaggcgc ttaacaggct gccaaacaag aattgcagta tccagtatgg ccaatcctgc
     1381 accgtccagc tagaaagacg cgcttgtaac cactgccagc gcaacagcct ttctcctagc
     1441 cgcctgactg aggaatacgg ctcagccagt tgatgcatta acaaggctgc cacaatgagg
     1501 tacagggcaa agatcatcat gtctgacatg gccactttct ggccgatggt aatggcgtcc
     1561 cagacaaaac caccaataaa aaacgatagc ggcaggacca tgcgactgcg tgccagccag
     1621 cgtgatagcc tggtaggggt tgggttttca gagagtgtca tacagataaa tcgaaataaa
     1681 catacaagac gtcggcattt ttatcacaga taacgcctag tacaatagtc ttttggacac
     1741 taaaaagtaa actttctttg ataatcaaat ttagcctgtt aaactttaaa ttatcttaag
     1801 aaacgtccgt taactatcaa tatacgttta gatatccctg catagaatac cagctagagt
     1861 acaaccgatc tattatcaag catgcgcgat ttagttagac agtttaaaca gaaagtaaca
     1921 gaactaaagt ctgtcagcaa tgagactgaa caggctttaa tccgtgttgt ttttgtcagc
     1981 tttatcacgc tctaccttct tctgaaccat agcagctatg agcccgtatt actctgcctg
     2041 atctatttat gctttggctt cctgatgttg tttaacatcc tgaaggatcc gaagaaaaat
     2101 gaaaaacggc aatggacagc catgattatg gacatcacgg ccacgtcatt ggagcaaatg
     2161 atttcaggct caatggctgg tgtctttatt ggggtctatt tgtggctgat tattggctac
     2221 ggcttgcgct atgggactaa atttttcaag ggctgttatg tttttagcct gattggcttt
     2281 ggcattacgc tttacctgaa cccgtattgg gtagagcatc agcatctggc gtatggtttt
     2341 ttcctcaccc tattgctgat cccgccgcac accttgcgcc tgcagatcag cctggaaaaa
     2401 gcgaccaagg aagctggcca ggccaacgaa gccaaaacaa acttcgtttc taacatcagt
     2461 catgaaatgc ggacacctct gaacggtatt attggcgcca gcgagctgct cgcgcaaacc
     2521 aagctggata gcaaacagtc tgaattgctc aaaatggttg ggacttctgc ctccagcctg
     2581 aaaaaactga ttaatgacgt gctggatatc agcaaaatcg aaaaaggcaa ggtggagctg
     2641 gaagacatca ccttcttttt accggacctg attcagcggc tgcaactcat gttccagctt
     2701 gaagtggaac gaaaacagtt atggctgaga tttgaccttg ccccggaaac agaacgtcac
     2761 tatgtgggca gcttgcatca cattgagcag gtattggtca acctgattgc caacgctgtt
     2821 aaatttacac agcatggcgg ggttgatgtc atggtcactg ataccaacca gctcacgttc
     2881 cgtatccgtg atactggcat agggatcaag tccgaggttt taccattgat ttttgatagt
     2941 tttacccagg cagacagcag catcaccagg cgctatggcg ggacaggcct gggaacttcg
     3001 attgcaaaac aactggtcga agtcatgggc ggcaatatcg atgtttcaag ccaggtgaat
     3061 attggcacca cctttgtggt cgctttgccg ctgatgcatg cagcagcccc agcgcaacca
     3121 gttgcagatg aaatcaacct cgataaccag tccaatgtgg tgccgttgag cagccacacc
     3181 aagttccgca aaaaaattcg cgtgctgatt gccgatgaca atgtcgtcaa ccggctgatc
     3241 ctgaatgaaa ctttgcaaaa aatgcactgc atcgtcaagg cggtcgataa cggcgatgcc
     3301 gcattggatg cactggagca tcatcagttt gacctgatga ttctggatta caacatgccg
     3361 gaaatgaacg gcctggaagt atttaatatt taccatgccc tgccaggcag cctgccatta
     3421 agaactgtca tactgacggc agatgcgact aaaactaccc aagaacgctg cttgcgtgct
     3481 ggcgtctacc aggttttgac caagcctgtt gtgtcacgtc aaatccagac cttgatcgag
     3541 gcaattgcga gcacgattga aactggtcaa gcagcacaag cagcacctgg ca
//
LOCUS       DATMSK010000042         4571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_191802,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000042 DATMSK010000000
VERSION     DATMSK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191802"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VNZ84_01340"
     CDS             complement(<1..1128)
                     /locus_tag="VNZ84_01340"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HWU65621.1"
                     /translation="MTSSVIQPSAMLGMLGGGQLGRFFVIAAHEMGYKVTVLDPDKNS
                     PAGMIADVHLCAAYDDVAALKTMAETCQAISTEFENVPAETLATLAKSTVVHPSADAV
                     AIAQHRVKEKTFFKEAGLPVGPFVPVTVEGDLPEDGEMYPAVLKVARFGYDGKGQARV
                     ASRAEAVAAFQAFKQETCVLEKMLPLDLEVSVVLARDVHGNIAAFPTAENSHLNGILD
                     ISIAPARCSEVIKANAQELAKKLATKLNYVGVLGVEFFVVGNQLLVNEIAPRPHNSGH
                     YTIDACVTNQFEQQVRVITGQALGSPRLHSHAVMVNILGDSWLYSGKQVEPAWDKAFA
                     HGDLKMHLYGKHEPRKGRKMGHFTVIGEETQAVLNAAKVARS"
     gene            complement(1125..1619)
                     /gene="purE"
                     /locus_tag="VNZ84_01345"
     CDS             complement(1125..1619)
                     /gene="purE"
                     /locus_tag="VNZ84_01345"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWU65622.1"
                     /translation="MSKPLVAIIMGSDSDWPVMKNAAQMLADFGVVYEARVVSAHRTP
                     DLMFQFAESAASNGFQVIIAGAGGAAHLPGMVAAKTTLPVLGVPVPSKHLQGQDSLLS
                     IVQMPKGIPVATFAIGDAGAANAGLFAVAMLANQDQLLAEKLATFRKKQAEKVHAMEL
                     SEKP"
     gene            complement(1690..2811)
                     /gene="proB"
                     /locus_tag="VNZ84_01350"
     CDS             complement(1690..2811)
                     /gene="proB"
                     /locus_tag="VNZ84_01350"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWU65623.1"
                     /translation="MSVSLIQGSKTLIVKVGSSLVTNDGQGLDKAAIAAWAEQISALV
                     KQGRQIVLVSSGAVAEGMQRLGWKKRPVEINELQAAAAVGQMGLVQMYEQCFSQHGLH
                     TAQLLLTHEDLADRNRYLNARTTLRTLLDLKVIPIINENDTVVTEEIRFGDNDTLGAL
                     VANLIEADALIILTDQQGLYSADPRKNPDAVFVNFETAGNPELEAMAGGAGSAVGTGG
                     MLTKILAAKRAANSGAHTVIASGREPNVLGRLSQGEAIGTHLKAGKMKTLAKKQWLAD
                     HLRVGGKLVLDDGAVNVLTKDGKSLLPIGVVDVLGQFERGDVVACVDAQGKEWARGIV
                     NYNANEVRRIKRKPSSEIEQTLGYVEEKELIHRDNLVLL"
     gene            complement(2854..3906)
                     /gene="obgE"
                     /locus_tag="VNZ84_01355"
     CDS             complement(2854..3906)
                     /gene="obgE"
                     /locus_tag="VNZ84_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWU65624.1"
                     /translation="MKFIDEATIKVYAGDGGNGVATFRREKYEPMGGPNGGDGGRGGS
                     IYVVADRNINTLVDYRYTRTFKAQRGENGSGSDCYGKGGDDMVLRVPVGTTISDKATG
                     QMMVDLNADGQKVLMAKGGNGGLGNIHFKTSTNRSPRQCTKGDPGESFELYLELKVLA
                     DVGLLGMPNAGKSTFIRSVSAAKPKVADYPFTTLHPNLGVVRVDSNRSFVIADVPGLI
                     EGAAEGAGLGHQFLRHLQRTSLLLHLVDLAPFDDGVDPVQEAKAIVNELKKYDQELYD
                     KPRWLVLNKIDMLEDSAKKVADFVKAYGWNGPVFPISAISGTGCQALTYAIMDHIDQA
                     RAQCEAEAEQSSTVIE"
     gene            complement(4017..4274)
                     /gene="rpmA"
                     /locus_tag="VNZ84_01360"
     CDS             complement(4017..4274)
                     /gene="rpmA"
                     /locus_tag="VNZ84_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWU65625.1"
                     /translation="MAHKKAGGSSRNGRDSQAKRLGVKAFGETVVNAGSIIVRQRGTK
                     MHAGENVGMGKDHTLYALVEGKVKFAVKGALKRHTVSIEAV"
     gene            complement(4322..>4571)
                     /gene="rplU"
                     /locus_tag="VNZ84_01365"
     CDS             complement(4322..>4571)
                     /gene="rplU"
                     /locus_tag="VNZ84_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWU65626.1"
                     /translation="EKLTGDVGSTVVIDKVLLIADGETTTIGAPLVAGAKVNATVVSH
                     GRGDKVMIFKMRRRKHYRKTQGHRQSYTELQIDTISAK"
BASE COUNT         1121 a         1306 c         1007 g         1137 t
ORIGIN      
        1 actgcgcgca actttggcag cattcaacac tgcctgcgtt tcctcaccaa tcacagtaaa
       61 atgccccatc ttgcggcctt tgcgtggttc atgtttgcca tataaatgca tttttaagtc
      121 gccatgcgcg aaagccttat cccacgctgg ttcaacctgt ttgccgctgt ataaccagct
      181 atcccccagg atattcacca tgaccgcatg gctatgtaac cgtggactac ccaatgcctg
      241 accagtaatc acacgcacct gctgctcgaa ctggttggtg acacaagcat caatcgtgta
      301 atggccagag ttgtgaggac gtggggcgat ttcattcacc agcaactggt tacccactac
      361 aaagaactcc acccccagca cccctacata attgagtttg gttgccagtt ttttagctaa
      421 ctcctgggca ttcgccttga tcacttctga acaacgtgcc ggcgcgatac tgatatcgag
      481 aataccattc aagtggctat tctctgcggt tgggaaggcg gcaatattgc catgcacatc
      541 acgcgccaac accacggaca cttccaggtc caatggcagc attttctcca gcacacaggt
      601 ttcctgcttg aatgcctgaa aagccgccac tgcttcagca cgactcgcaa cacgcgcctg
      661 acctttgcca tcgtaaccaa agcgcgccac tttgagcacg gccggataca tctcgccatc
      721 ttccggcaag tcaccctcaa cagtcaccgg tacaaatggt ccaaccggca aaccagcctc
      781 cttgaagaag gttttttctt tcactctatg ctgcgcaatc gcgaccgcat cagcactcgg
      841 atgtactacg gtagattttg ccaaagtagc cagcgtttcg gctggtacat tttcaaactc
      901 agtagaaatg gcctggcagg tttctgccat ggttttcaaa gcagctacat cgtcgtacgc
      961 ggcacataaa tgcacatctg caatcatgcc ggctggacta ttcttgtcag gatccagcac
     1021 ggtgacttta taacccatct cgtgggctgc aatcacgaaa aaacggccaa gctggccgcc
     1081 gcccaacata cctaacatag cagaaggttg aatcacggat gatgtcatgg cttttcactc
     1141 aactccattg cgtgtacttt ttcggcctgt ttcttgcgga aagtcgccaa cttttcagcc
     1201 aacagctgat cctggtttgc caacatggcc accgcaaaca agccagcatt cgcggcaccg
     1261 gcatcaccaa tggcaaatgt cgctactgga atgcccttag gcatttgtac aatagataac
     1321 agggagtcct gaccttgtag atgcttggaa ggcacaggca cacccaatac tgggagcgtg
     1381 gttttggctg ccaccatccc tggcaagtgg gcagccccac cggcaccggc aataatcacc
     1441 tggaacccat tgcttgcagc agattcggca aactggaaca tcaggtcagg cgtgcgatgt
     1501 gcagacacca cgcgcgcctc atagacgaca ccaaaatctg ccagcatttg cgcggcattt
     1561 ttcatcaccg gccagtcact gtccgacccc ataataatag ccaccaaagg tttactcata
     1621 ttgttacctt aaaaaacgat gcggtaatta ctgcgatgta aacaccgcgt gtatctcaat
     1681 gcaatctggc tacaacagaa ccagattatc cctgtgaatc aattcttttt cttctacgta
     1741 gcccagtgtc tgctcaatct cgctacttgg cttgcgcttg atgcggcgta cctcattggc
     1801 attgtaattg acaatgccac gtgcccactc tttaccctgc gcatccacac aggccaccac
     1861 atcgccgcgc tcaaactggc ccaagacgtc gaccacgccg ataggcagta agcttttgcc
     1921 atccttagtg agcacattga ctgcaccatc atcaagcacc agtttgccac ccacgcgcaa
     1981 atgatctgcg agccattgtt tcttggccag cgttttcatt ttgcccgctt ttaagtgtgt
     2041 gccaatcgct tcgccctgac tcaggcgacc aagtacattg ggctcacgac cagaggcaat
     2101 caccgtgtgc gccccactat tcgcagcccg tttggcggcg agaattttgg tcagcatacc
     2161 gcccgtacca acagcgctac cggcaccacc ggccatggct tccaactcag gattaccggc
     2221 cgtttcaaaa ttgacaaaga cggcatcagg atttttacgt ggatccgcgg aatacaaacc
     2281 ttgctgatcg gtcaggataa tcagcgcatc cgcttcaatc aggtttgcga ccagcgcccc
     2341 caacgtgtca ttatcgccaa agcggatttc ctcagtgacc accgtatcat tttcattgat
     2401 gataggaatg accttgaggt caagcaaggt acgcaatgtt gtgcgtgcat tcaagtagcg
     2461 attgcggtct gccaggtctt catgtgtcag cagcaattgc gctgtatgca ggccatgctg
     2521 tgaaaagcat tgctcataca tttgcaccaa ccccatttga ccaacggctg ccgccgcttg
     2581 taattcgttg atctcaacag gacgcttttt ccaacccaga cgctgcatgc cttcagccac
     2641 cgctccgctg gagacaagca cgatttgccg cccctgtttg accaacgcac tgatttgctc
     2701 agcccaggca gcaatagcag ctttgtccag tccttgacca tcattggtca ccaggctgga
     2761 accgactttg acaatcaggg ttttgctgcc ttgaattaaa gaaacactca tacctgccaa
     2821 gaaattctta aaatgaatgc gtttttataa tgattattca atcacagtcg acgactgttc
     2881 tgcctcagct tcacactggg ctcgggcctg atcaatatga tccatgatgg cataagtcag
     2941 cgcctggcaa cctgtgccac taatggccga aataggaaac acaggaccat tccagccata
     3001 agccttcaca aaatcggcga cttttttcgc actgtcttcc agcatatcaa ttttgttgag
     3061 gaccagccag cgaggcttgt cgtaaagttc ctggtcgtat tttttcagct cattcacaat
     3121 cgctttggct tcctgcaccg gatcaacgcc gtcatcaaat ggcgcaaggt caaccaaatg
     3181 tagcaacaag gaagtacgtt gtaagtgacg taaaaactga tgtcctaaac ccgcgccttc
     3241 tgccgcgcct tcaatcaggc ctggcacgtc cgcaatcaca aagctacgat tgctatccac
     3301 gcggactacg cctaaattag ggtgcagggt ggtaaatgga taatcagcga ccttaggttt
     3361 cgccgcagat actgaacgaa taaaagtaga cttaccggcg ttgggcatgc ctagcaaccc
     3421 cacatcggcc aggaccttca gctcaagata cagctcgaaa gactcgcctg gatcgccttt
     3481 ggtacattga cgtggagaac ggttggtact ggttttgaaa tgtatattgc ccaagccgcc
     3541 attgccgccc tttgccatca acactttctg accatcagca ttcaaatcca ccatcatttg
     3601 gccagtggcc ttatcactaa tggtggtacc gacgggcacg cgcaacacca tatcgtcgcc
     3661 acctttgccg tagcagtcag aaccgctgcc attttcacca cgttgcgcct tgaaggtacg
     3721 tgtataacgg taatctacca aggtattgat gttgcggtct gccactacat agatggaacc
     3781 gccacgacca ccatcaccac cattggggcc acccataggc tcatactttt cgcgccggaa
     3841 cgtggcaaca ccgttgccac cgtcaccggc gtagactttg atggtcgctt cgtcaataaa
     3901 cttcatagtg gatttgctcg ttattcagct atcaggtgat atggcaggga tcgcagcaca
     3961 aaactataaa tgaaaaagcc ccgtcaagcg acagggcttt tttcataaaa gtgcgattaa
     4021 accgcttcta tgctcacggt atgacgcttc aaagcgcctt ttacagcaaa tttaactttg
     4081 ccttctacca atgcatacag ggtatgatct ttacccatgc caacgttttc gccagcgtgc
     4141 attttcgtac cgcgttggcg aacaatgatg ctgccagcgt taactactgt ttcaccgaaa
     4201 gctttaacgc ccaggcgttt ggcttgtgag tcgcgaccgt tacgtgaact accgcctgct
     4261 tttttgtgtg ccatgatgga ttccttattc tagcaatctg ctctactcga gcagaacgtg
     4321 cttattttgc tgaaatagtg tcgatttgaa gttctgtata gctctggcga tgaccttgag
     4381 tcttgcggta gtgcttacga cggcgcattt tgaagatcat cactttgtcg ccacggccat
     4441 gtgataccac agtggcattc actttagcac cggctaccaa aggtgcacca atcgtagttg
     4501 tctcgccgtc agcaatcaac aacactttgt cgattacaac agttgaacct acgtcgccag
     4561 tcaatttctc a
//
LOCUS       DATMSK010000043         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_403278,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000043 DATMSK010000000
VERSION     DATMSK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403278"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VNZ84_01370"
     CDS             <1..674
                     /locus_tag="VNZ84_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU65627.1"
                     /translation="KLAEQGINVVIGVRDQHKGEQAVTALKALGAEADYVIYDTSMPD
                     AAQTVANTLESRYGKLDILINNAGILKEDLIGQNNSLTVDQDTLKTTFEANFFAVIAL
                     TQALLPLLQRSPAGRIVNLTSILGSQTLHSTPNSPIEVAKGLAYNASKSALNMFTIHL
                     AHALKDTAIKVNSAHPGWVKTELGGPHAPMEISDSWKTSVRLATLDEQGPNGGYFHED
                     QTLPW"
     gene            668..916
                     /locus_tag="VNZ84_01375"
     CDS             668..916
                     /locus_tag="VNZ84_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HWU65628.1"
                     /translation="MVNRAHQRVALKRKLVFAMLMSLVTVNIVSLSILLMNHVPASRF
                     LEKWFASVTVAWPIVFISILLVAPALLRLTERLIKEES"
     gene            1001..1336
                     /locus_tag="VNZ84_01380"
     CDS             1001..1336
                     /locus_tag="VNZ84_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWU65629.1"
                     /translation="METKHAVTLLASLAQEARLAIFRSLVQAGPDGLSAGTLSQALQI
                     PNSTLSFHLKELNHAGMISARQESRFIYYSANYASMNALLAYLTENCCAGQAPCCPDL
                     QCQPTQTKE"
     gene            1341..2375
                     /gene="arsB"
                     /locus_tag="VNZ84_01385"
     CDS             1341..2375
                     /gene="arsB"
                     /locus_tag="VNZ84_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HWU65630.1"
                     /translation="MSTVKNISSFERHLTWWVAGCIMVGIAVGKAAPGLFHLIAAVKL
                     AEVNLPVAILIWFMIIPMLIKVDFTAMREVFTHARGIAVTLAINWLVKPFSMALLAWL
                     FIRHAFAAWLPHDQIDSYIAGLILLAAAPCTAMVFVWSNLCRGEPKFTLSQVAINDTI
                     MLFAFAPIVALLLGLSSIVVPWKTLLISVLLFIIVPVLLSQWLRAWLLKRGQAAFDHT
                     QQRIQPFSMAALLLTLVLLFAFQGEQILNQPVIIALLAVPILIQVYFNALLAYWLNKK
                     FKVAHCVAGPSALIGASNFFELAVATAIALFGFNSGAALATVVGVLIEVPVMLSIVAI
                     VNRTRHWYES"
     gene            2381..2854
                     /locus_tag="VNZ84_01390"
     CDS             2381..2854
                     /locus_tag="VNZ84_01390"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWU65631.1"
                     /translation="MHVLFLCTGNSCRSIIAEAVFNHLAPAHMRAMSAGSQPTGEVHP
                     RSIALLQSKGISTEGYYSKSWNEISLKPEIVITVCGNAANETCPAYLGNAIRAHWGVD
                     DPAKATGSEVQIQASFEQAYRVLRGRIEAFLQLNDSILNDHQALQANLLAIGQLE"
     gene            complement(2878..>3096)
                     /gene="infA"
                     /locus_tag="VNZ84_01395"
     CDS             complement(2878..>3096)
                     /gene="infA"
                     /locus_tag="VNZ84_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWU65632.1"
                     /translation="IEMQGAVTEILPDARYRVKLDNGHELIAYTGGKMRKHKIRILAG
                     DKITIEMSPYDMDKGRIIFRHLEPRKPL"
BASE COUNT          760 a          786 c          738 g          812 t
ORIGIN      
        1 agaaactggc ggagcaaggc atcaacgtag tgatcggagt gcgcgaccag cacaaaggtg
       61 aacaggcagt gaccgcattg aaagccttgg gagcagaggc agattacgtc atttacgata
      121 caagcatgcc tgatgctgcg caaacagtcg ccaacacgct ggaatcccgt tacggtaagc
      181 tggatattct gattaataat gctggtatcc tcaaagaaga cctgatcggt cagaataaca
      241 gcctgacggt agatcaagac acgctcaaaa ccacctttga agctaacttc ttcgccgtca
      301 ttgccctgac tcaagcgcta ttgccattgt tgcaacgttc ccctgcaggc cgtatcgtca
      361 acttaaccag catcctgggt tcacaaacgt tgcacagcac gccaaactcg ccaatagaag
      421 tcgccaaagg cttggcctac aatgcatcaa aatctgcatt gaatatgttt accattcatt
      481 tggcacatgc gctgaaagac actgccatta aagtcaactc tgctcatcca ggctgggtaa
      541 aaacagaatt gggcgggcca catgcgccaa tggaaatcag tgatagctgg aaaacttcag
      601 tcaggttggc cacgctggat gaacaaggcc ctaatggcgg atacttccac gaagaccaaa
      661 cattgccatg gtaaaccgtg cccatcaacg tgtggcgctg aagcgcaaac tggtgtttgc
      721 catgctgatg tctcttgtca cagtgaatat cgttagtctg tctatcctgc tgatgaatca
      781 tgtgccagct tcacgttttc tggaaaaatg gttcgccagt gtcactgttg cctggcctat
      841 cgtttttatc agtattctgc tggtcgcacc tgctttattg cgcttaacag agcgtttaat
      901 taaagaagaa tcttagaaga cagcccctgg aaatcagggc tgtcattcct gcgtttgtga
      961 tgaccttgct acatttcgat atttatggaa atatagcatt atggaaacaa aacatgctgt
     1021 cactttactc gcatccctgg ctcaggaagc gcggcttgcc atcttcaggt ctctggtaca
     1081 agcgggccct gacgggctct ccgcgggcac gctttcccaa gcgttgcaaa ttcccaatag
     1141 cacgttgtct ttccacctca aagaattgaa ccatgccggg atgatcagcg cccggcaaga
     1201 aagccggttt atctattact cagccaatta tgcaagcatg aatgccctgc tggcgtatct
     1261 cactgaaaac tgctgtgccg ggcaagcccc ttgctgcccc gacttacagt gccagcccac
     1321 gcagacaaag gaataagagg atgtctacgg taaaaaatat cagcagcttt gaacgtcacc
     1381 tgacttggtg ggtcgctgga tgcatcatgg ttggcattgc ggttggcaaa gcagcacctg
     1441 ggttatttca cctgattgca gccgtaaaat tggccgaggt taatttaccc gtggctatcc
     1501 tgatatggtt catgattatc ccgatgctga tcaaggtcga tttcactgcc atgcgggagg
     1561 tctttaccca tgccaggggg attgccgtga cgctagccat taactggctg gtaaaacctt
     1621 tttctatggc gttactggct tggctattta tccgccatgc ttttgctgcg tggctacccc
     1681 acgaccaaat tgatagctat attgccgggt taatattgct cgcagcggcc ccatgtaccg
     1741 ccatggtatt tgtctggagt aatttgtgcc ggggtgagcc taaattcacc ttatcccaag
     1801 tggccattaa cgacaccatc atgctgtttg cctttgcacc gattgtagcg ttattactgg
     1861 gcctttccag tattgttgtg ccatggaaaa cactgctgat ctcagtcttg ttatttatta
     1921 ttgtgcctgt cttgctaagt caatggctgc gtgcatggct cttgaaaaga ggccaggccg
     1981 catttgatca cacacaacaa cgtatacagc cgttctccat ggcggcattg ctgctcaccc
     2041 tagtgttact cttcgcgttt caaggagagc aaatcctaaa tcaacctgtc atcattgcat
     2101 tactcgccgt gcctattctg atacaggttt attttaatgc cctgcttgcg tactggctca
     2161 acaagaaatt caaggttgcc cattgtgttg caggcccttc cgccctgatt ggcgcttcca
     2221 atttttttga actggcggtc gccactgcta ttgcgctgtt cggattcaat tcgggcgctg
     2281 ccctggcaac tgtggtgggt gtgctgattg aagtcccggt catgctctcc attgtagcca
     2341 tcgtcaaccg cacccgtcac tggtatgaaa gttaatgcag atgcacgtct tgttcttatg
     2401 tactggcaac tcctgtcgct ccattattgc cgaagcagta tttaatcacc tcgcccccgc
     2461 ccatatgcgc gccatgagtg ccggtagcca accaacaggc gaagtgcatc cgcgctcgat
     2521 cgccctgctg caatcaaagg gtataagcac cgaaggctat tacagcaagt cctggaatga
     2581 aatatcactt aaaccagaga tcgtgattac cgtgtgtggt aatgctgcca acgaaacgtg
     2641 tcctgcgtat ttaggcaatg ccatacgcgc acactggggt gtagacgacc cagccaaagc
     2701 aacgggtagt gaagtacaga tacaggccag ttttgagcag gcctatcgag tactcagggg
     2761 acggattgag gcatttctac aattgaatga ctcaatactg aatgaccatc aggctttaca
     2821 ggcaaattta ctggccattg gtcagcttga gtaggcgtcg ttgaaaacga cgcgatatta
     2881 tagaggcttg cgcggctcta aatgacggaa aatgatgcgg cctttatcca tatcgtaagg
     2941 cgacatttca atggtaattt tatcaccagc caggatgcgg atcttatgtt tacgcatctt
     3001 gccgcctgta taagcgatca actcatgacc attatccagt ttcacgcgat agcgtgcgtc
     3061 aggcagaatc tcggtcactg caccttgcat ttcaat
//
LOCUS       DATMSK010000044         4963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_144851,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000044 DATMSK010000000
VERSION     DATMSK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144851"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VNZ84_01400"
     CDS             <1..880
                     /locus_tag="VNZ84_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWU65633.1"
                     /translation="SYALRPRRSMLYVPGCNQHYLERARTLAADSVILDLGDPILIDA
                     KIQSRHNVVNAVKESGYGKREVVVRVNDLHSIWGHDDIQAVANIGADAILFPNIESRE
                     DVLQAQQLLDEAGGKNVPMMVMIESPLAVLNAKEIAAASDRVICMVMATSDLISQLHA
                     RVTHERSAILTSLSLVILAARAYGKGVIDGITSDFKNMHSFEYACRLGRDIGMDGKSL
                     VHPAQIAYCNDAYTPQRAEVEHARLIIKALKEANEAGRGTVVVNDKLVEHHHIKAAQR
                     LIQLAESIAELEEEFI"
     gene            891..1949
                     /locus_tag="VNZ84_01405"
     CDS             891..1949
                     /locus_tag="VNZ84_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWU65634.1"
                     /translation="MSTKINTGRFFEHFVLGETIQHATPRTITAGDTALYIALTGARN
                     PLHCSEPLAKSLGYVQMPIDDLLAFHIAFGKTVPDISVNAVANLGYADVRFIQPVYIG
                     DTLSTASEVIGLKQNSNGKSGVVWVRSTTVNQHQQPVLSWVRWVMVHKKNQEVPAPET
                     VIPELPEFVSPESLAVPAHFTLNSDLGTATGGQCWWDDYEVGECMRHPSGLTINESDH
                     TMATHLYQNNARLHFDAHLMQSTPQGQCLVYGGVVISACRALSYEGLENAIAIVAING
                     GTHANPSYSGDTIYCMTKVLDKWSLPHPELGALRLRMVGVKNMPPATLENIKTTQGYH
                     PHVVLDLDYTVLIPRKGN"
     gene            1952..2950
                     /locus_tag="VNZ84_01410"
     CDS             1952..2950
                     /locus_tag="VNZ84_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWU65635.1"
                     /translation="MAVHPNQALFGGEKPFPVIPACEHFAGSEKLILKALSLQDTANP
                     NGVGPVFDITCDCEDGAASGQEQAHAEMIVRVLNSEANKYKMAGARIHDYTHPHWKKD
                     IDILVGGAGEVLSYITIPKCTDIAQAHEMITYIQQVAKQHGITREIPVHILIETHGAL
                     RQVHEIAQLPWLQVLDFGLMDFVSAHHGAIPATAMRSPGQFDHRLLARAKTEVVSAAL
                     ANGVVPAHNVTLDLKNVEVTYSDASRARNEFGFMRMWSIYPTQIQAIVDAMKPNYDEV
                     TDAANILLAAQAAEWGPIQYAGELHDRATYRYFWEVLQKAKQTGVTMPEDAEHAFF"
     gene            2975..3853
                     /gene="prpB"
                     /locus_tag="VNZ84_01415"
     CDS             2975..3853
                     /gene="prpB"
                     /locus_tag="VNZ84_01415"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HWU65636.1"
                     /translation="MTQINTAGGRFRAALAAEKPLQIIGAINAYHAMLATQSGFKAIY
                     LSGGGVAAGSLGLPDLGISGLEDVLVDVRRITDAVDTPLLVDIDTGFGGAFNIARTIK
                     SVAKAGAAAVHIEDQVSAKRCGHRPNKAIVSLDEMVDRVKAAVDAKPYDDFVVMARTD
                     ALAVEGLQSAIDRACACVEAGADMIFPEAMTDLDMYQQFVDAVKVPVLANITEFGSTP
                     LFTVNELATAGVGMVLYPLSAFRAMNQAALTVYQGVRKDGTQKNVLDLMQTRADLYEH
                     LGYHAFEQKLDALYKK"
     gene            3891..>4963
                     /gene="prpC"
                     /locus_tag="VNZ84_01420"
     CDS             3891..>4963
                     /gene="prpC"
                     /locus_tag="VNZ84_01420"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HWU65637.1"
                     /translation="MNTTTDPTTPATDAPKRKKGVALAGVAAGTTAICTVGHSGNDLH
                     YRGYDIIELAKHATFEEVAYLLIHGELPTQPQLTAYHQKLKKLRGLPSALKAVLEQIP
                     ANAHPMDVMRTGCSMLGTLEPEAVDRNTAGAQALGDRLIACFGSILIYWYHFSHRGVR
                     IDVETDDDSIAAHFLHILHGKKPSELHIKAMNTSLVLYAEHEFNASTFTSRVIAGTLS
                     DLYSCITGAIGALRGPKHGGANEAAMEIIERYQTPDEAEADIKARMARKEIIIGFGHP
                     VYTIADPRNESIKGVSKSLCEDAGNMKLFDISARIEEIMWNEKKMFPNLDWYSASSYH
                     MMGIPTGMFTPIFVISRTTGWVA"
BASE COUNT         1246 a         1296 c         1283 g         1138 t
ORIGIN      
        1 gtcatatgca ctcagaccac gacgctcgat gctatatgtg ccaggttgca accaacatta
       61 cctggaacgt gcacgcacgc ttgcagcgga ctcggttatc ctggacctgg gagaccctat
      121 cctgattgat gccaaaatcc agtcgcgcca caatgttgta aacgcggtga aagaaagtgg
      181 ttacggcaaa agagaagtcg tggtacgggt caatgacctg cactccattt gggggcatga
      241 cgacattcag gctgtcgcca acattggtgc cgatgccatt ctgtttccca atattgaatc
      301 gcgtgaagac gtgctgcagg cgcagcagtt actggatgaa gctggcggca aaaatgtgcc
      361 gatgatggtc atgattgaaa gcccgcttgc ggtcttgaat gccaaagaga tcgctgccgc
      421 ctctgatcgc gtgatctgta tggtgatggc gacgtctgac ctgatttcac aattacatgc
      481 acgcgtgacc catgagcgtt ccgcaatttt aacttcttta tctttagtga tcctggcggc
      541 acgcgcttac ggcaaaggcg tgattgacgg tattacttca gacttcaaga acatgcattc
      601 gtttgaatat gcctgccgtc tgggtcgaga catcggcatg gatggtaagt ccttggtgca
      661 tccggcacaa attgcttatt gcaatgacgc ctacaccccg caacgagcag aagtcgagca
      721 cgcaaggttg attatcaaag cgctcaagga agccaatgaa gccggtcgtg gcacggtggt
      781 cgtcaacgac aaattggttg aacatcatca tatcaaagcc gcacaacgct tgattcaact
      841 ggcagagtcc attgccgagc tagaagaaga gtttatataa ggagttgaac atgagcacaa
      901 aaatcaacac cgggcgcttt tttgaacatt ttgtactggg tgaaaccatc cagcacgcca
      961 cgccgcgcac gatcactgct ggcgacacag cactttatat tgcactgacg ggcgcacgca
     1021 atcctttaca ttgcagtgag ccactcgcaa aatccttggg ctatgtgcag atgcctattg
     1081 atgatttgct ggcatttcat attgcctttg gcaagacggt ccctgatatc tcagtcaatg
     1141 ccgtggctaa cctgggttac gcagacgtgc gctttattca gccagtttac attggcgaca
     1201 ccttaagcac cgctagtgaa gtcatcggtt taaagcaaaa ctccaacggt aagagcggtg
     1261 tagtgtgggt acggtcaact acagtcaacc agcaccaaca acccgtactc agctgggtgc
     1321 gctgggtgat ggtgcacaaa aaaaatcagg aggttccagc ccctgagacc gtgattccag
     1381 agttaccgga atttgtcagc cctgaatcac tcgcggtacc ggcgcatttc acactaaaca
     1441 gcgaccttgg caccgccaca ggtggccagt gctggtggga tgactacgag gtaggcgaat
     1501 gtatgcgtca tccttccggc ctgacgatta atgagagtga ccacaccatg gccacgcatt
     1561 tataccagaa caatgcgcgc ctgcattttg atgcgcactt gatgcaaagc acgccgcaag
     1621 gtcaatgcct ggtctacggc ggcgtagtca tctcggcatg ccgtgcactg agttatgagg
     1681 gcctggaaaa cgcgatcgct atcgttgcca ttaatggcgg cacacacgcc aatcccagct
     1741 actcaggtga cacaatttat tgcatgacca aggtgctgga caaatggtcc ctgccccacc
     1801 ccgagctagg tgcattgcgt ttacgcatgg tgggggtcaa aaacatgccg ccagcgaccc
     1861 tggaaaacat caaaaccacg caaggctacc acccgcacgt ggtacttgat cttgactata
     1921 ccgttcttat tccacgcaaa ggaaactaaa catggctgtt catccaaacc aggctctttt
     1981 tggcggtgag aaaccgtttc cggtcattcc ggcttgtgag cattttgccg gcagcgaaaa
     2041 gctgatcctg aaagccctgt cattgcaaga cacagccaac ccgaatggtg tcggcccggt
     2101 atttgatatt acctgcgatt gtgaagacgg tgcagccagt ggacaggaac aggcacatgc
     2161 cgaaatgatt gtgcgcgttc tcaactcgga ggcgaacaag tataagatgg ccggagcacg
     2221 tattcacgac tacacgcatc cgcactggaa aaaagacatc gacattctgg tcggtggcgc
     2281 gggtgaggta ttgagctaca tcactattcc taaatgtacc gatatcgcgc aagcgcatga
     2341 aatgatcacg tacattcagc aagtggctaa gcaacatggc atcacacgcg aaatcccggt
     2401 acacatcctg attgaaaccc acggtgcact gagacaagtc catgagattg cacaactgcc
     2461 gtggttgcaa gttctcgact ttggcctgat ggattttgtc agcgcccatc acggcgccat
     2521 ccctgccacc gcgatgcgta gtcctggtca gtttgatcac cgcttgctgg cccgagccaa
     2581 aacagaagtg gtatcagcgg cgttggccaa tggtgtggta ccagcacaca atgtgaccct
     2641 ggacctgaaa aatgtggaag tgacctatag cgatgcttcc cgcgcccgca atgagtttgg
     2701 ctttatgcgg atgtggagta tttatccgac ccagatccag gctatcgttg atgccatgaa
     2761 accaaactat gacgaagtca ctgacgctgc caacatcttg ctggcagccc aagccgccga
     2821 atgggggcca attcagtatg caggtgaatt gcatgaccgt gccacctatc gctacttctg
     2881 ggaagtgttg caaaaagcca agcaaactgg tgtcaccatg ccggaagacg ctgaacacgc
     2941 tttcttttaa ctgcatcgca acaggatcaa caacatgacg caaatcaata ctgccggtgg
     3001 tcgtttccgc gcggcactgg cagcagaaaa accgttacag attatcggtg caattaacgc
     3061 ctaccacgcc atgctggcca cccagtccgg gtttaaagcc atctatttat caggcggcgg
     3121 cgtcgcagca ggatcgttag gcctgccaga cctcggcatc tccggcctgg aagatgtgct
     3181 agtcgatgtg cgccgcatta ccgatgcagt agatacaccg ttattagttg atatcgacac
     3241 cggctttggt ggggctttta acattgcacg cacgattaaa agtgtcgcca aagcaggtgc
     3301 agcagcagtg catattgagg atcaagtcag cgccaaacgc tgcggtcacc gtccgaataa
     3361 agcaatcgtc agcctggacg aaatggtaga ccgcgtgaaa gctgccgtcg atgccaagcc
     3421 gtatgatgat tttgtcgtta tggcgcgtac ggatgcgcta gcggttgaag gtttgcaatc
     3481 ggcgatcgac cgcgcctgtg cttgcgttga agccggcgca gatatgattt tcccggaagc
     3541 catgacagac ctagacatgt accagcagtt tgtcgacgcc gtcaaagtgc ctgtgctggc
     3601 caacattacg gagtttggtt ctacaccttt gtttacggta aatgagttag ccactgctgg
     3661 cgtcggcatg gtgctgtatc cattgtctgc cttccgtgcc atgaaccagg cagcgctcac
     3721 ggtctatcag ggcgtgcgta aagatggtac gcaaaagaat gtgctggact tgatgcagac
     3781 acgtgccgat ttgtatgagc atctgggcta tcacgctttt gaacagaaac tagacgcgct
     3841 atataagaaa tagatcgcgt taccagactg agtgatttaa ggaggttttt atgaatacca
     3901 ccactgatcc caccactcct gccaccgatg cccctaaacg caaaaagggc gtggcgctgg
     3961 cgggcgtagc tgcaggtacc acggccattt gtaccgtggg tcatagcggc aatgacttgc
     4021 attaccgtgg ttacgatatc atcgagctgg ctaaacatgc cacattcgaa gaagtcgctt
     4081 atttgttgat acacggcgag ttgcccaccc aaccgcaact caccgcttat catcaaaagc
     4141 tcaaaaagct acgcggcctg cccagtgcac ttaaagccgt actggaacag attcctgcca
     4201 atgcgcatcc catggatgtg atgcgcacgg gttgttcaat gttggggacg ctggaaccgg
     4261 aagccgttga ccgcaatact gcgggcgcac aggcattggg tgaccgcctg attgcctgtt
     4321 tcggctccat cctcatttac tggtatcact tcagccaccg tggcgtgcgc attgatgtcg
     4381 aaactgatga cgactcaatt gctgcccact tcctgcatat cctgcatgga aaaaaaccgt
     4441 ccgagctaca cattaaggcc atgaatactt cgctggtatt gtatgcagag catgagttta
     4501 atgcctctac tttcacctca cgcgtgattg caggcacctt gtctgacctg tattcctgca
     4561 tcactggtgc cattggtgca ttacgaggcc ccaaacatgg tggtgccaat gaagcggcga
     4621 tggagattat cgaacgctac cagacgcctg atgaagcgga agcggatatc aaagcccgca
     4681 tggctcgtaa agagatcatt attggttttg gtcacccagt gtatacgatt gccgacccac
     4741 gcaatgaatc catcaagggc gtgagcaaat cactctgtga agatgccggc aacatgaaac
     4801 tgtttgatat ttcagcccgc attgaagaaa tcatgtggaa cgaaaaaaag atgttcccca
     4861 acctcgattg gtacagcgcc tccagctatc acatgatggg catccctacc ggtatgttca
     4921 caccgatttt tgtgatttcc cgcacaacgg gctgggtggc aca
//
LOCUS       DATMSK010000045        14354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_403441,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000045 DATMSK010000000
VERSION     DATMSK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 14354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14354
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403441"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VNZ84_01425"
     CDS             <1..352
                     /locus_tag="VNZ84_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HWU65638.1"
                     /translation="NDAVEILNKKGAWLQIKTAGKTGWVRLLTVKRNSSSGNNVAGVV
                     GVATGRSGTGKVVSTTGIRGLSAEDLKTAQFNEAETKKLESNTMSAEEARKYAISGGL
                     SSHQYPYFTEGGQQ"
     gene            349..1251
                     /locus_tag="VNZ84_01430"
     CDS             349..1251
                     /locus_tag="VNZ84_01430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWU65639.1"
                     /translation="MKHSSLKRTLMLASLTGMVCGSAAALDLKGALKDAVNKQLGGDN
                     TTEQATPASNNNASAQDKLANINWKNPSQEDEIAVGRNVAGTILGASPLVNDSQLQQY
                     VNKVGRWVANQSERTSLPWHFGVIDSSDVNAFAAPGGYILITKGLYQRLTSESQLAGV
                     LGHEIAHVVQRHHLKVMQKQALMDAGTSFLKEKISKNKITQRAVSTGAEISARNLDKS
                     AEFEADSMGTVLAARAGYDAYGLAEVLQDMETIGNNDGSVALLFQTHPHPDDRLAQLS
                     LVSGDQMDNISGGKTLEKRLYKLK"
     gene            complement(1310..2314)
                     /locus_tag="VNZ84_01435"
     CDS             complement(1310..2314)
                     /locus_tag="VNZ84_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65640.1"
                     /translation="MTQTQRFEIATYILMAVLMWLVLQVQLLPALLAGLLVFELIHVI
                     SPLIARKLPGRYAKWLAVVLIATLVIGLLVALGMGIVTLIRSDAGGLAVLFDQLAKII
                     EDSRKVLPPWLNERLPDDAITLQQTVIEWLRTHSADWQLLGKEAGHHLAHILIGMVIG
                     AMIALHDVQTKVNHKPFAQSLLQRITLFAQAFKNIVFAQVKISAVNAALTAVYLVIIL
                     PLCGVHLPFTKTMIAITFLVGLLPVIGNLVSNTVIVIVSTSHSFVVAMGSLAFLIIIH
                     KLEYFLNARIVGGQIRAYAWELLLAMLLMEATFGLPGIVAAPVYYAYVKSECRERGWV
                     "
     gene            complement(2342..2902)
                     /locus_tag="VNZ84_01440"
     CDS             complement(2342..2902)
                     /locus_tag="VNZ84_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HWU65641.1"
                     /translation="MQTFSQLGQADQTINKSRFIARARRCENERELALFLRQFASEHQ
                     TAGHLAYAFRMKTAEGIVARFSDAGEPAGTAGKPIMQMLDGQNYVNCCVAVVRYYGGI
                     NLGTGGLVRAYGGTAKQAMEQAGSMPFVEMVQLKISTTYKRVDELTREVQKLHGEILD
                     KQFGENVDFLLKLPLEAATQLQARFE"
     gene            complement(2913..4556)
                     /locus_tag="VNZ84_01445"
     CDS             complement(2913..4556)
                     /locus_tag="VNZ84_01445"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWU65642.1"
                     /translation="MLSDIDIAQAATLQPIMDVAMKVGLSSYDLHLYGQHMAKLTQAT
                     VNRLQSQPSKAKLILVTAINPTPAGEGKTTTTVGLTDALNQAGYHALACLREPSLGPV
                     FGMKGGATGGGYAQVVPMENINLHFTGDFHAISAANNLLAALIDNHIYHGNALGFDQV
                     SWRRCMDMNDRALRRITLENEVHTGFDITVASEVMAIFCLATDLTDLTRRLGRIQVGI
                     NKQGKPILASDLQAEGAMTALLKDAFQPNLVQTLEGSPALLHGGPFANIAHGCNSLVA
                     TQTALRLADYVVTEAGFGADLGAEKFMDIKCRQSGLRPDVAVIVATIRALKYNGGVEV
                     ADLNREDLSALALGIANLEKHIANLRQHFDLPVVVALNHFTADTGAEVALVQTVVESL
                     GASLHVCRHWAQGGAGALDLAHAVAAQAQKTSAPRLLYADDMPLPEKLTTIAQAMYGA
                     SHVTYSEVAQQQLTQLAEAAHGLPVCVAKTQYSLSCDAKLRNVPKNHVLHVRELRLSR
                     GAGFVVAICGDIMTMPGLPKQPASARIGVDADGKITGLS"
     gene            complement(4590..6083)
                     /locus_tag="VNZ84_01450"
     CDS             complement(4590..6083)
                     /locus_tag="VNZ84_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HWU65643.1"
                     /translation="MVQPMLIAKNNETDSVMLPKMANRHGLIAGATGTGKTVTLQSLA
                     EQFSRIGVPVFMADVKGDLSGMSQVGGGNAKVEARVQQLGLTDHANKAYPVMFWDMFG
                     EKGHPVRTTLSEMGPLLLARMLNLNDVQGGVLNAIFKIADDNGMLLLDMKDLRAMAQH
                     CAENSATYQTQYGNISTASVGAIQRALLQLETEGGDILFGEPALNLEDMMQTDSKGLG
                     FINILAADKLLNSPRVYATLLLWILSELFEKLPEVGDMEKPKLVFFFDEAHLLFNDAP
                     AILMQKIEQVVRLVRSKGVGVYFVSQNPLDIPDVVLGQLGNRVQHALRAFTPRDQKAV
                     QVAAQTFRANPKLDVATAITELGVGEALVSFLDEKGAPTPVERVFVCPPESRIGPATD
                     TERKQAMAQSLVAGVYDKVVDRESAYEILKQRTEQAAAASQAPAQKEQEGSGFDLGGV
                     LSGKSSRSDSVLESAAKSAARAIGSQLGRVIIRGVLGSILGGSKTKR"
     gene            complement(6191..6733)
                     /locus_tag="VNZ84_01455"
     CDS             complement(6191..6733)
                     /locus_tag="VNZ84_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HWU65644.1"
                     /translation="MRLLILKMLCCLGLILSLPACAGPANLRVIGHGMPDVDLDNAIY
                     EVPVKSGVTYQDLIDSLKSISEGMNFVNPANFPIGDHMKQRGQDPQGVKEVRTFCNLS
                     MGTEIILDHPEFLVFAPCRLAIYEKPDASGKRQLYLGLDRPTHDLKNIKHPTARAQAS
                     AQQLEDALIELMEKARRGDF"
     gene            complement(6806..7732)
                     /locus_tag="VNZ84_01460"
     CDS             complement(6806..7732)
                     /locus_tag="VNZ84_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HWU65645.1"
                     /translation="MKFHQLRYVHEVVRQQLNISAASEVLHTSQPGVSKQIQLLEEEL
                     GLQIFQRNGKRLIGVTEPGRKIVELSARVMLDMQNIKRVGDEYSREDTGELTIATTHT
                     QARYRLPAAVKQFIAQYPNVKLTIHQGNPAQVTAMVASGEADVGIATESISNDERLSC
                     LACYEWNRCLVVPPQHPLLSKATLTLKDLADYPLITYDFAFTGGTLVSKVFHDAGLQP
                     NVVFTAIDADVIKTYVNLGLGIGLIANMAYDEMRDAPLKSLDCSHLFPNSTTYLGVRR
                     DAFLRDYMLNFITMLSPQYDKRTVQRALHQDL"
     gene            7839..8609
                     /locus_tag="VNZ84_01465"
     CDS             7839..8609
                     /locus_tag="VNZ84_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWU65646.1"
                     /translation="MEFGYILAGFVVGFLVGLTGVGGGSLMTPILLLLYNIKPAVAVG
                     TDLLYASVTKSVGIFAHGKLGNIDWTIVKRLAIGSVPASMITLWALHSMDLQSGNVVS
                     TIKFWLGIALIVTSVAVLLRNRLMSLVAKEEWIPARYVPGATVILGIVLGALVTLTSV
                     GAGALGVTALILLYPKEKITTIVGSDIAHAVPLTLVAGLGHASLGTIDYQLLGTLLIG
                     SIPGIYLGSHMSSRVAEHWVRIALAMILIFVGFKLVFH"
     gene            8651..10315
                     /locus_tag="VNZ84_01470"
     CDS             8651..10315
                     /locus_tag="VNZ84_01470"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWU65647.1"
                     /translation="MYKYDPIDQQIVNERVAQYRDQTRRYLAGELTDEEYRPLRLQNG
                     LYIQRHAPMLRIAVPYGLISSKQLRKLGDIAKKYDRGYGHFSTRQNLQLNWPKLEDTP
                     DILAELATVEMHAIQTSGNCIRNITTDQFAGIAPDEIIDPRAIAEIMRQWSTFHPEFA
                     LLPRKFKIAVSGTEKDRAIVQAHDIGLEFYKDAEGQMAIKVWVGGGLGRTPILGTVIR
                     EHLEWQHVLSYCEAVIRVYNIHGRRDNAYKARIKILVKALGIEEFKRQVEEEWAHLKD
                     GPLTITQNELDRVAKYFEPMPYKTLPAHDAAFDAAVSSNPAFAAWVKRCVQPHRVPGY
                     RAVTLSLKPHGKAPGDITSEQMHVVADLADSYSFGELRSSHEQNLILADVQLSDLFAV
                     WEKARAAGLATPNIGLLTDIICCPGGDFCSLANAKSIPIAEAIQMQFDNLDYLYEIGD
                     LELNISGCMNACGHHHVGHIGILGVDKDGSEWYQVTVGGKQGNDASIGTVIGPSFSAE
                     EMPGVVQRLIEVYVRERHDEERFIDTVRRLGVAPFKAHVYAEKETA"
     gene            10315..10932
                     /locus_tag="VNZ84_01475"
     CDS             10315..10932
                     /locus_tag="VNZ84_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HWU65648.1"
                     /translation="MSQLIQLAANGQASIVADAWTRVIPPAAGEESVRKQAGKVVLFK
                     LTGEQTYTADQIANTQIPATGKILVPLTIWQARKAELQPRLAAGELGIVLATHETAEA
                     LQAEFPDLNVLPLIAVFVERFADGRIFTLGNWLRTRFGFKNELRAIGDVLRDQLFFHK
                     RAGFNSFLIRADRSAEDALASLNDFSQPYQGGVADVPVWRRVNRA"
     gene            10929..11687
                     /locus_tag="VNZ84_01480"
     CDS             10929..11687
                     /locus_tag="VNZ84_01480"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWU65649.1"
                     /translation="MSFGVSMLKPAANNPATRPELSDALVASVHAKRAQAIALLQAAA
                     AEFPAITFANSYGAEDMVLTDMIAKENLPIEIFSLDTGRLPAETYTLMGDVEQTYSIK
                     PLVFFPKHEAVENYVRTQGINAFYESIDLRKACCHMRKVEPLQRALTGKQAWITGMRA
                     EQAATRSNLPTREYDAGNQLEKFNPLSDWTEKEVWAYIRLFEVPYNALHDQFYPSIGC
                     APCTRAVAMGEDIRAGRWWWENPDSKECGLHVKK"
     gene            11710..12639
                     /gene="cysD"
                     /locus_tag="VNZ84_01485"
     CDS             11710..12639
                     /gene="cysD"
                     /locus_tag="VNZ84_01485"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HWU65650.1"
                     /translation="MSTKQPLSHLDWLEAEAIHILREVAGQCANPVLLFSGGKDSLCI
                     LRLAEKAFRPGRFPFPLMHIDTGHNYQEVINFRDQRAAELGERLIVRSVEDSMKRGTV
                     VLKTPDEPRNKHQSVTLLEAIEEFGFDCCIGGARRDEEKARAKERIMSFRDEFGQWDP
                     KNQRPELWNLYNARAHKGENIRAFPISNWTEMDVWQYIEREKLELPSIYFAHQRDIVM
                     RQGAIFPVNVPLASGEVINQPKAGEEVINMQVRFRTVGDITCTAPVISDANDVSKIVV
                     ETATTTITERGATRLDDQTSDASMEQRKKEGYF"
     gene            12641..13915
                     /gene="cysN"
                     /locus_tag="VNZ84_01490"
     CDS             12641..13915
                     /gene="cysN"
                     /locus_tag="VNZ84_01490"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HWU65651.1"
                     /translation="MTTQHNDSLLRFMTCGSVDDGKSTLIGRLLYDTKTILADTLNNI
                     ARTSQKRGMEAVDLSLLTDGLQAEREQGITIDVAYRYFSTGTRKYIIADAPGHEQYTR
                     NMVTAASTANLAIILIDARKGVLTQTRRHSYLAHLVGIPHIVVAVNKMDLVNYDQATY
                     EKIKADYLAFATEIGLAQAREIKFIPMSALNGDMLVDRLDNMNWYQGETLLDILEAAP
                     AAHSEQAEQFRFPVQFVCRPHDSANPELHDFRGFMGRVESGSIAVGDAVTVLPNGYQS
                     KVKAIQLGNQQLESAKTEQSITLLLEDEIDTSRGDMIVKSSEVPEAVKQIEAHVCWLS
                     ETPLSPARTYIVRHTTRESKAKIGSIQYKVDVNTLEQLATADLKMNDIARLSFKLAQP
                     LMVDSYDKNRATGAFIVIDESTNNTVGAGMIV"
     gene            14002..14325
                     /gene="fdxA"
                     /locus_tag="VNZ84_01495"
     CDS             14002..14325
                     /gene="fdxA"
                     /locus_tag="VNZ84_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HWU65652.1"
                     /translation="MTYVVTENCIQCKYTDCVDVCPVDCFVEGPNFLAINPDECIDCT
                     LCVAECPAEAIFSEDDVPADQQHFIALNARLATVWPTITARKEPLPDADAMNGKAGKT
                     ELLVE"
BASE COUNT         3725 a         3835 c         3538 g         3256 t
ORIGIN      
        1 aaacgatgcc gtggagatcc tcaacaaaaa aggggcctgg ctacaaatta aaacggcagg
       61 caaaaccggt tgggtacgct tgctcacggt aaaacgcaat agcagcagtg gcaataatgt
      121 tgcgggtgtg gtgggtgtcg ccactggacg cagcgggacc ggtaaagttg tgtctaccac
      181 cggcatccgt ggattaagcg ccgaagacct caaaaccgca caattcaatg aggcagagac
      241 caagaaactc gaaagcaata caatgagcgc tgaagaagcc agaaaatatg caatcagcgg
      301 tggcttaagt agccaccagt atccatactt tacagaaggt ggtcagcaat gaaacattcc
      361 tctttaaaac ggacactaat gctggcctct ttgactggca tggtatgcgg aagcgcagca
      421 gctctggatc tgaaaggcgc acttaaagat gctgtaaaca agcaattagg tggcgacaat
      481 accaccgagc aagcgactcc agcatccaat aataacgctt cggcacagga caaactggcg
      541 aatatcaact ggaaaaatcc tagccaggaa gatgagattg ccgtgggccg taatgtagcg
      601 ggaacgattt tgggtgcctc accgttagtg aacgatagcc aattgcagca atatgtgaac
      661 aaggtgggcc gctgggtggc caaccagtca gagcgcacga gtttgccctg gcattttggc
      721 gtcatcgaca gcagcgatgt caatgccttt gcagctccag gcggctacat attgatcaca
      781 aaaggccttt accaaagact gaccagtgaa agccagctgg caggtgtgct cggtcatgaa
      841 attgcacatg tggtgcagcg gcatcatttg aaagtgatgc aaaaacaagc actgatggat
      901 gcaggcactt cttttctcaa agaaaaaatc agcaagaata aaatcaccca acgcgctgtt
      961 tcaacagggg cagaaatcag tgcgcgtaat ctggataaaa gcgctgaatt tgaagcagac
     1021 agcatgggca ccgtactggc tgcccgtgca ggttacgacg cctatggcct ggcagaagtg
     1081 ttacaggaca tggagaccat tggcaacaat gatggcagtg tggccttgct gttccagacc
     1141 caccctcatc cggatgacag actggcgcag ttgagcctgg tgagcggcga ccagatggac
     1201 aatatcagtg gcggtaaaac ccttgaaaag cggttgtata agttaaagta acccagcaaa
     1261 aataaatagg gcccgccaag gcgggcctta tttattggca gactgaaggt tatacccagc
     1321 cgcgttcacg gcactcactt ttcacgtaag cataatagac tggcgcggcc acgatccccg
     1381 gtaagccaaa cgtcgcctcc atcagcaaca tggccagtag caactcccag gcataggcac
     1441 ggatctggcc acccacaata cgggcattca ggaagtattc tagcttatgt atgatgatca
     1501 ggaatgccag tgaccccatt gccaccacaa atgaatgact ggtactgaca atcacaatca
     1561 cggtattgga aaccagatta ccaatgaccg gcaataaacc gaccagaaag gtgatggcaa
     1621 tcatggtttt tgtaaacggc aaatgtacac cacataacgg tagtatgatc accaggtaga
     1681 cagcagtcaa tgccgcattg actgctgaaa ttttcacctg cgcaaatact atgtttttaa
     1741 aggcttgcgc aaacagggtg atacgttgca acaggctttg tgcaaatggt ttgtggttga
     1801 ccttggtttg tacgtcgtgc aaggcaatca tcgcgccgat caccatccca atcaggatat
     1861 gtgccaaatg atgcccggct tccttgccca gcaattgcca gtcagcggaa tgcgtacgca
     1921 accactcaat cacggtttgc tgcaaggtaa tggcatcgtc tggcaagcgt tcattgagcc
     1981 aaggtggtaa tactttgcgc gagtcctcaa taattttggc caattgatca aatagcactg
     2041 ccaggccacc ggcgtcactg cgaatcagcg tcacgatccc catccccagc gcgaccagca
     2101 ggccaatgac caaggtagca atcagcacta ctgccagcca ttttgcgtat ctccccggca
     2161 gtttgcgggc gatgagagga gaaatgacat gaattaattc aaataccagt aacccggcca
     2221 gcaaggcagg cagtaattgc acctgcaata ctagccacat caacactgcc atcaggatat
     2281 aagtggctat ctcaaatcgt tgggtctggg tcacactggc tccggtcttg tatcaggtca
     2341 atcactcaaa tctggcttgc aattgcgtgg cggcctctag tggcaatttg agcaaaaaat
     2401 cgacattttc cccaaattgc ttgtccagga tttctccatg caacttctgt acttcccgcg
     2461 tcaactcatc aacgcgctta taggtagtgg atattttcaa ctgcaccatt tccacaaaag
     2521 gcatactgcc cgcctgctcc atagcctgtt tcgcggtgcc gccgtaagca cgtaccaggc
     2581 caccagtgcc caagttaata ccaccataat agcgcacgac agcgacacag caattgacat
     2641 aattttgccc atccagcatt tgcataatgg gtttgcccgc cgtcccggca ggttcaccag
     2701 catctgaaaa acgcgccaca atgccctcag ctgttttcat cctgaaggca taagccagat
     2761 ggccggctgt ctggtgctcg ctggcaaact ggcgcaagaa taaggcgagt tcacgttcgt
     2821 tttcacaacg tcgggcacgt gcaataaaac gggatttatt aatggtttga tcggcctggc
     2881 cgagctggct aaaggtttgc atggcagaaa cttcaggaaa ggccggttat tttaccatcg
     2941 gcatccacac cgatgcgcgc actggctggc tgttttggca accccggcat ggtcatgata
     3001 tcgccgcaaa tagccaccac gaaaccggcc ccacgcgaca aacgcaattc gcgtacatgc
     3061 aggacatgat tttttggcac attgcgcagc ttggcatcac aagacaagga gtattgcgtt
     3121 ttagcgacac agacaggcaa accgtgagca gcttcggcca attgtgtaag ttgctgctgc
     3181 gccacttcgc tgtatgtcac atggctggca ccgtacatcg cttgcgcaat cgtggtgagc
     3241 ttttcaggca atggcatatc atctgcatac agcaagcgcg gcgcagatgt tttttgcgct
     3301 tgtgcagcca ccgcatgcgc taaatccaac gcgcccgcgc cgccttgggc ccaatggcgg
     3361 cacacatgca ggctggcacc cagcgactcc actactgtct gcaccaaggc cacctcagca
     3421 ccggtatcgg cagtaaaatg attcaacgct acgaccactg gcaagtcaaa gtgctgacgc
     3481 aggttagcga tatgtttttc caggttggcg atgcccaatg ccaacgctga caagtcctcg
     3541 cgattcaggt cagccacttc tacaccgccg ttgtatttca aggcacgtat ggtcgccaca
     3601 atcaccgcca catccggccg caagcctgac tggcggcact tgatgtccat aaacttctca
     3661 gcgccgaggt cggccccaaa cccggcctcg gtgacgacat aatctgccag acgcaaagct
     3721 gtttgggttg ccaccagcga attgcaaccg tgtgcaatat tggcaaacgg gccaccatgt
     3781 agtaacgctg gcgaaccttc cagcgtctgc accaggttag gttgaaaggc atctttgagc
     3841 aatgcagtca tcgcgccctc tgcctgcaaa tcactggcca aaatgggttt gccctgttta
     3901 tttattccca cctggatacg tcccagcctg cgtgttaaat ctgtcaaatc ggtcgccaga
     3961 cagaaaatcg ccatgacctc gctggccacc gtaatatcaa agccggtatg cacttcattt
     4021 tccagcgtaa tccggcgcaa ggcgcggtca ttcatatcca tgcaacggcg ccaactcact
     4081 tgatcaaaac caagcgcatt accatggtaa atgtggttat caatgagggc tgccagcaag
     4141 ttattggcgg cactgatggc atggaagtca cccgtaaaat gcaggttgat gttctccatg
     4201 ggcaccacct gcgcataacc gccgccggtc gccccaccct tcatgccaaa cacaggcccc
     4261 aacgaaggct cacgcaaaca ggccagcgca tggtaaccgg cctgattcaa ggcatccgtg
     4321 aggcctaccg ttgtggtggt tttgccttca cctgcaggcg tcggattaat cgccgtcaca
     4381 aggattagct tggcttttga cggctgtgat tgcaagcggt ttacggtcgc ttgcgtcaat
     4441 ttggccatat gttggccata cagatgcaaa tcatacgacg aaaggcccac tttcatcgcg
     4501 acatccataa tcggctgtag cgtcgcggct tgggcaatgt cgatatcact taacatgcaa
     4561 aaatcctgat tgattaacgg tcacaccagt tagcgcttgg ttttgctgcc tccaagtata
     4621 gagccaagca cacctcggat aatgacacga cccaattgcg aaccaatggc acgcgccgca
     4681 gacttggctg cagattccag cacagaatcc gaacggctgc ttttaccgct taaaacgccg
     4741 cctaaatcga atcctgaacc ctcctgctct ttttgcgcag gtgcttgaga ggccgctgct
     4801 gcctgctcgg tacgctgttt gagaatctca taagcggatt cacggtctac caccttgtca
     4861 tacacgcccg ccacaagcga ttgcgccatg gcctgtttgc gttcagtatc ggtggccgga
     4921 ccaatccgac tttctggcgg acagacaaat acgcgctcaa ccggcgtcgg ggcacctttc
     4981 tcatccagga acgacaccag cgcttcaccg acccctaact cggtaatcgc agttgcgaca
     5041 tccagtttgg gattagcgcg gaaggtttgc gctgcaacct gtaccgcctt ctggtcgcgt
     5101 ggcgtaaacg cgcgcagcgc atgctgaaca cggttaccca attgaccaag caccacatct
     5161 ggaatgtcca gcgggttttg cgacacgaaa tagaccccta cccctttgga acgcaccagg
     5221 cgcaccactt gttcaatctt ttgcatcaag atggcaggcg catcattaaa taacaggtgc
     5281 gcctcatcaa agaaaaacac caatttcggt ttttccatat cacccacttc gggcaacttt
     5341 tcaaacagtt cggacaatat ccacagcaat agcgtcgcat agacacgtgg tgaattcagc
     5401 aatttatccg cggccagaat attgataaac cccaatccct tactatcggt ttgcatcatg
     5461 tcttccagat taagcgcggg ctcaccaaac aagatatcac cgccttcggt ttccagttgc
     5521 agcaatgcgc gctggatagc gcccacactg gcagtagaaa tattgccgta ttgtgtttgg
     5581 taagtggctg aattttcagc acaatgttgc gccatcgcac gcagatcttt catatccagc
     5641 agcaacatgc cattgtcatc tgcaatcttg aaaatggcat ttagcacacc accctgcaca
     5701 tcattcagat ttaacatgcg cgccaacagc aacggcccca tttcgctcag cgtggtacgc
     5761 accggatgcc ctttttcacc gaacatatcc cagaacatca ctggataggc tttattggca
     5821 tgatcggtca agccaagctg ctgtacgcgt gcttcaacct tggcattacc tccgccgacc
     5881 tggctcatgc cagacaagtc ccctttcaca tcggccataa acacgggcac gcctatgcgt
     5941 gagaactgct cggccaaact ttgcaaggtc accgttttac cggtaccggt tgcgcctgca
     6001 atcaggccat ggcggttagc catttttggc agcattacac tgtcagtctc attatttttg
     6061 gcgatcagca taggttgaac catgggatat actcgaacaa ttaaagttaa gttgagtata
     6121 ggcgaaggcc taacaaagtg gaaggcagtc tgatgaaaac acagggattc cgtgaaagaa
     6181 tctgtgttga ctaaaaatcg ccgcggcggg ctttttccat gagctcgatt aaagcatctt
     6241 cgagttgttg cgcagaggcc tgcgccctgg cagtcggatg cttgatattt tttaaatcat
     6301 gcgtaggccg gtccagaccc aggtaaagct gacgcttgcc actggcatcc ggcttttcgt
     6361 aaatggccag gcggcaagga gcgaagacca ggaactcggg atgatcgaga ataatctcgg
     6421 ttcccatact taaattacaa aaggtccgca cttctttgac cccttgcggg tcctgcccac
     6481 gttgtttcat gtggtcacca atcgggaaat tggctggatt gacgaagttc atgccttccg
     6541 agatcgattt caggctatca atcaagtcct ggtaagtgac accggatttc accggcacct
     6601 catagattgc attatccaga tccacatcag gcataccgtg cccaatgaca cgcaggttag
     6661 cgggtccagc acaggccggc aaactcaaga tcaagcccag gcaacacagc atcttcaaga
     6721 tcaataagcg catgacagcc ctcttaatag tccatatcga tagacgcatt acagcatgcg
     6781 gccaatcact tgagtgtgac caacatcaca agtcctggtg caaggcgcgc tgcactgtgc
     6841 gtttgtcata ctgcggggac agcatggtaa taaagttgag catgtagtcg cgcaaaaacg
     6901 catcacggcg cacgccaaga taagtcgtac tattagggaa caaatggctg caatccaggc
     6961 ttttaagcgg tgcatcccgc atctcgtcat acgccatatt ggcaatcagg ccaatcccca
     7021 accccaggtt aacataggtt ttaatcacgt ctgcatcaat cgcggtaaat acgacattcg
     7081 gctgcaaacc tgcatcatga aacaccttgg acaccaaggt accgccggta aaggcaaagt
     7141 cataagtaat caagggatag tcggctaggt cttttagcgt cagcgtggct ttggacaaca
     7201 aaggatgctg cggtggcacg accaggcaac ggttccattc ataacaagcc aggcaggaca
     7261 aacgctcgtc attactgata ctttccgtgg caatcccgac atcggcctca cccgacgcaa
     7321 ccatggcagt gacctgggcc gggttaccct gatggatggt cagcttcaca tttggatatt
     7381 gcgcaataaa ctgctttacc gctgcaggca aacggtaacg cgcctgagta tgcgtggtgg
     7441 caatggtgag ttcacccgta tcttcacggc tatattcatc gccgacccgc ttgatattct
     7501 gcatatccag catgacccgc gctgataact ctacgatctt gcggccaggc tccgtcacac
     7561 ctatcagacg tttaccgttg cgctgaaaaa tctgcaatcc cagctcttct tcgagtagct
     7621 gaatctgttt gctgacacct ggctgggagg tatgcaatac ttcagaggct gcagaaatat
     7681 tcaattgttg ccgcacaacc tcatgtacat agcgtaattg gtgaaatttc attacccact
     7741 cgatataacc aaaagctata tcatagaaca tttaattatt aaatcaatca ttaaaagctt
     7801 gttatcatcc tgcggttata gttttcaaga tactcctcat ggaattcgga tacatactgg
     7861 caggatttgt ggttggcttt ttggtgggtt taacaggggt aggcggtggc tcattaatga
     7921 cgcctatcct gctgctactt tacaatatca aaccggccgt cgctgtaggg acagacctgc
     7981 tttatgcgtc agtcaccaaa tctgtcggca tctttgctca tgggaaattg ggcaatattg
     8041 actggaccat cgtcaaacgc ctggcaatag gcagcgtacc cgcctccatg atcacactat
     8101 gggccctgca tagtatggat ctgcaatcag gcaatgtggt ctcaaccatc aagttttggc
     8161 tcggcattgc attgattgtg acctcagtcg ccgtgctatt gcgcaacaga ctgatgtcgc
     8221 tagtcgcaaa agaagaatgg atcccagcgc gctatgtgcc aggtgctacg gtcatactgg
     8281 gcattgtact gggtgcgctg gtcaccctga cttcagtcgg cgctggcgcc ttgggcgtca
     8341 ctgcgctgat tctgctgtac ccaaaagaaa aaattaccac cattgttggc agcgatattg
     8401 cacacgccgt accactgaca ctggtggctg gattagggca cgcctctttg ggcaccatag
     8461 actaccaatt attgggcacc ctgctcattg gctccatccc tggtatctat ctcggcagcc
     8521 atatgagttc ccgcgtcgca gagcattggg tacgcattgc gctggctatg atattgattt
     8581 ttgtaggctt taagctggtt ttccattaaa atattgcctc ttcgcttttt gcgaacaaag
     8641 tgactcaagc atgtataaat acgatcctat agaccaacaa attgtgaatg aacgtgtcgc
     8701 ccaataccgc gaccaaacgc gccgctacct ggctggcgaa ttaactgatg aagaataccg
     8761 cccgctgcgt ttgcaaaacg gcttgtatat tcagcgccat gcgcctatgt tgcgtatcgc
     8821 cgtgccttac ggcctgatat cgagcaaaca attacgtaaa ctcggcgata ttgcaaaaaa
     8881 atatgatcgc ggctatggcc atttcagtac acgccaaaac ctgcaactca actggccaaa
     8941 actggaagac acgccagata tactggctga gcttgccacg gttgagatgc atgccatcca
     9001 gacttccggc aactgcatcc gcaacatcac gactgaccaa tttgctggta ttgccccgga
     9061 tgagatcatt gatccacgtg caatcgccga gatcatgcgt cagtggagta ccttccatcc
     9121 tgaattcgcc ctgctgccac gcaagttcaa aatcgctgtg tcaggcacag aaaaagaccg
     9181 cgccattgtg caagcacacg atattggcct cgagttctat aaagatgcag aaggccaaat
     9241 ggccattaag gtctgggttg gtggcggttt gggccgcaca cctatcttgg gcaccgtgat
     9301 ccgcgaacac ctggaatggc aacatgtgtt gtcttactgc gaggccgtga tccgcgtgta
     9361 taacattcat ggccgccgcg acaatgctta taaagcgcgt atcaagattc tggtgaaagc
     9421 actaggcatt gaagaattca aacgccaggt ggaagaagag tgggcacacc ttaaagatgg
     9481 cccattaacc atcacgcaaa acgagctgga tcgcgtggcc aaatacttcg agcctatgcc
     9541 atacaaaacg ttgccagcac atgatgctgc atttgatgca gccgtgtcca gcaacccggc
     9601 atttgctgcc tgggtcaagc gttgcgtaca accacaccgc gtgccaggct accgtgcagt
     9661 gacactgtca ctcaagccac acggcaaagc gcctggcgat atcaccagcg agcaaatgca
     9721 tgtagtggca gacctggcag actcctacag ctttggcgaa ttgcgttcct cgcacgaaca
     9781 aaacctgatt ctggccgatg tacaattgtc tgaccttttt gccgtatggg aaaaagcccg
     9841 tgccgcaggc cttgctacgc caaacatcgg tttactgacg gacattattt gctgcccagg
     9901 tggcgatttc tgcagcctgg ccaacgccaa gtccatcccg attgctgaag cgatccagat
     9961 gcaattcgac aacctggatt acctgtacga aatcggtgac ttggaactca atatttccgg
    10021 ttgcatgaac gcttgcggtc accaccacgt tggccacatc ggcattttgg gtgtggataa
    10081 agatggttcc gaatggtatc aggtcacagt cggcggtaaa caaggcaatg acgccagtat
    10141 aggcacagtg attggcccct cattctcggc agaagaaatg ccgggcgtgg tacagcgcct
    10201 gatcgaggtc tatgtacgtg agcgccatga tgaagagcgc ttcattgata ccgtgcgtcg
    10261 cttgggtgtc gccccattca aagcccatgt ttacgctgaa aaggaaactg cataatgagc
    10321 caactgattc aattggccgc gaatggtcag gccagcatag tcgccgacgc ctggacccgt
    10381 gtaataccac cagccgcagg cgaggagtct gtacgcaaac aagcgggtaa agtcgtgtta
    10441 ttcaaactca ccggcgaaca aacctatacg gcagaccaaa ttgccaatac gcagatccct
    10501 gccacaggta aaattctggt gccgctgacc atttggcaag cacgcaaagc agaactacaa
    10561 cctcgcctgg ctgctggtga actcggtatt gtattggcga cgcatgaaac ggctgaagcc
    10621 ttgcaggctg agtttccaga cctcaacgta ttgccattga ttgccgtatt tgtagaacgc
    10681 tttgccgatg gccgcatctt tacattgggt aactggctgc gcacccgttt tggttttaaa
    10741 aacgaactgc gcgcgattgg cgacgtattg cgtgaccaac tgtttttcca taaacgtgca
    10801 ggtttcaaca gctttttgat ccgtgctgac cgctccgcgg aagatgcact ggccagcctg
    10861 aatgacttca gtcagcctta ccagggtggc gtggcagatg tgccagtctg gcgcagggtg
    10921 aaccgcgcat gagtttcggc gtcagcatgc tcaaaccggc cgccaataac ccggccaccc
    10981 gtccagagtt atcagacgca ctggttgcca gcgtacacgc caaacgtgca caagcaattg
    11041 ctctgttgca agccgcggcg gctgaatttc cggccatcac atttgccaac agctatggtg
    11101 ctgaggacat ggtcttaact gacatgattg ccaaagaaaa tctgccaatc gagatattct
    11161 cgctggatac gggccgcctg cctgctgaaa cctatacgct gatgggcgat gttgagcaaa
    11221 cttacagcat taaaccattg gtttttttcc ccaagcacga agccgtggaa aattacgtgc
    11281 gcacgcaagg tatcaatgcc ttttatgaaa gcatagactt gcgtaaggcc tgctgccata
    11341 tgcgtaaggt agaacccttg cagcgtgcac tcaccggcaa gcaagcctgg atcactggta
    11401 tgcgtgccga gcaggcagct acgcgtagca acttacccac acgcgaatac gatgcaggca
    11461 accagctgga aaaattcaac ccgctgagtg actggacaga aaaagaagtc tgggcttata
    11521 tccgcctgtt cgaggtccct tataacgcct tgcatgacca gttttacccc agcattggtt
    11581 gcgccccttg tacgcgcgcc gtcgccatgg gtgaagatat ccgcgctggt cgctggtggt
    11641 gggaaaaccc agacagcaaa gagtgcggtt tgcacgtcaa aaaataaatt cgtaacgatt
    11701 gaaataatca tgtctacaaa acaaccttta agtcatttgg actggctgga agctgaagcc
    11761 attcatattt tgcgcgaagt ggcaggtcaa tgcgccaacc cggtattact cttttcgggc
    11821 ggcaaagact ctctgtgcat cttgcgcctg gcagaaaaag cgttccgccc tggccgcttc
    11881 cccttcccgc tcatgcacat tgacaccggt cacaactatc aggaagtgat taacttccgt
    11941 gaccaacgtg cagctgaatt gggtgaacgc ctgattgtgc gctcagtaga agactccatg
    12001 aaacgcggta ccgtcgtatt gaaaacgcca gatgaaccgc gcaacaaaca ccagtctgtc
    12061 accttgctcg aagcgattga agagtttggt tttgactgct gcattggcgg tgcccgccgt
    12121 gacgaagaaa aagcccgcgc caaagagcgt atcatgagtt tccgcgatga atttggccaa
    12181 tgggatccga aaaaccagcg ccctgaattg tggaacctgt acaacgcccg cgcgcacaaa
    12241 ggtgaaaaca ttcgcgcgtt cccaatctca aactggaccg agatggatgt ctggcagtat
    12301 atcgaacgcg aaaaacttga gctgcccagc atctacttcg ctcatcagcg cgatatcgtc
    12361 atgcgtcaag gcgcaatttt cccggtcaat gtgccactgg cctcaggcga agtgatcaac
    12421 cagcctaagg ctggcgaaga agtgatcaat atgcaggtac gcttccgcac tgtgggcgat
    12481 atcacttgca ccgccccagt catttccgat gcgaatgatg tgagcaaaat cgtagtggaa
    12541 acagcaacaa cgaccattac cgagcgcggc gcgacccgtc tggacgacca gacctcagac
    12601 gcctctatgg aacagcgcaa gaaagaaggt tatttctaat atgacgacgc aacacaacga
    12661 ctctttgctg cgctttatga cttgcggcag cgtggatgac ggcaagagca ccttgattgg
    12721 tcgcctgctg tatgacacca aaacgattct ggccgatacg ctgaacaata ttgcgcgtac
    12781 ttcgcaaaaa cgcgggatgg aagcggtgga tttgtcttta ttgacggacg gcctgcaagc
    12841 cgagcgtgag caaggtatta ccattgacgt tgcctaccgt tatttcagca cgggcacccg
    12901 caaatacatc attgccgatg caccaggcca cgagcaatac acccgcaaca tggtgactgc
    12961 agcatctact gccaacctgg cgattatctt gatcgacgca cgcaaaggcg tactgacaca
    13021 aacccgccgc catagttacc tggcacattt ggtaggcatt ccacatatcg tggtggctgt
    13081 aaacaagatg gacctcgtta actacgatca ggcaacgtat gagaagatca aggcagacta
    13141 ccttgcgttc gcgactgaaa ttggtctggc acaggcacgc gagatcaaat ttatcccaat
    13201 gtcagccctc aacggtgaca tgctggtaga ccgcctggac aacatgaatt ggtaccaggg
    13261 tgaaaccctg ctcgacatcc tggaagcagc acctgctgcc catagcgaac aagctgagca
    13321 gttccgcttc ccggtacagt ttgtctgccg tccacatgat tcagccaatc ctgagcttca
    13381 cgacttccgt ggttttatgg gacgtgttga atcaggttcg attgcggttg gtgatgcagt
    13441 gactgtactg cccaatggct atcaatctaa agtgaaagcc atccagctgg gtaaccagca
    13501 actggaaagt gcaaaaaccg agcaaagcat cactctgttg ctggaggacg aaattgacac
    13561 atcgcgtggc gatatgatcg tcaaaagtag cgaggtacca gaggcggtca aacagatcga
    13621 agcacatgtc tgctggttgt cagagacgcc actttcccct gcgcgtactt acattgtgcg
    13681 tcataccacc cgtgagtcca aagctaaaat tggtagcatt caatacaagg tcgatgtaaa
    13741 caccctggaa cagctggcca cggccgatct taaaatgaat gatattgcgc gtctcagctt
    13801 taaactggca cagccactca tggtggacag ctacgacaag aaccgcgcta cgggtgcttt
    13861 tatcgtcatc gacgagagca ccaacaatac cgtgggcgcg ggcatgattg tttgatgagt
    13921 ggcaaaacaa cacatcgtca tttaagctaa attgcaataa aatatcattt tggctgaaat
    13981 agccggcaaa ggatttaaaa catgacttat gtagtaaccg aaaattgtat tcaatgcaaa
    14041 tataccgact gcgtagatgt gtgcccggta gattgttttg tggaaggtcc gaacttcctg
    14101 gcgatcaatc ctgacgaatg cattgactgc accctgtgcg tcgcagaatg ccctgcagaa
    14161 gccattttct ctgaagatga tgtgccggcc gaccaacagc actttattgc cctcaacgcg
    14221 cgcctggcaa ccgtatggcc gacgattaca gctcgtaaag agcctctgcc agatgcagat
    14281 gcgatgaatg gcaaagcggg taaaactgaa ttgctggtcg agtaatccag catatctgcc
    14341 aacattaaaa aagc
//
LOCUS       DATMSK010000046        12878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_74371,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000046 DATMSK010000000
VERSION     DATMSK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12878
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74371"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..504
                     /locus_tag="VNZ84_01500"
     CDS             79..504
                     /locus_tag="VNZ84_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HWU65653.1"
                     /translation="MVHFTPLQSLIGGLLIGFSTLLMIRLLGKIAGISGIVGQLWSAG
                     IGDRAWRFAFVAGLLVSPLLYALFSPLPETSVVTSTPWLIVAGLLVGFGSRLGSGCTS
                     GHGVCGLSRLSLRSLVATLTFMATAILVVWLLRHVFLIG"
     gene            514..933
                     /locus_tag="VNZ84_01505"
     CDS             514..933
                     /locus_tag="VNZ84_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HWU65654.1"
                     /translation="MVMLIALLAGIIFGFGLIVGGMTNPAKVLAFLDITGDWDPSLAF
                     VMLGAIAVGFFAFRAAARKPVSLLGMPVQLPGTRLIDRKLLLGAVLFGTGWGIAGFCP
                     GPAVASVLTGGVAVWLFVASMLAGMALFTLATRRGWI"
     gene            complement(940..2304)
                     /locus_tag="VNZ84_01510"
     CDS             complement(940..2304)
                     /locus_tag="VNZ84_01510"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWU65655.1"
                     /translation="MEQTTPDFFSQSESLTRAQGESLARMISLIMPDAAVFTVDAHKR
                     INYWSPGAEKLLGFTQPSLLGENCLTANRCEQCMRGCGLTESGKVEGAPLLLHRVDGT
                     RQAVLKYAMAYLPENGEFAGGLEILLPQQADPATSTGQSSLGEKYGLISHAPEMQKIF
                     DMIRRVAATDIPVLIRGDSGTGKELVARAIHGESTRHGAPFIAINCATLNAGMLESEL
                     FGHVKGAFTGAIRDHQGLFGRAEGGTLFLDEIAELPYELQAKLLRVLETGEYLPVGGS
                     QPMLANVRIVAATHKALREEVKAGRFRQDLLFRLRVVPIFLPSLQARKQDIPLILRHF
                     LKQQFTGEQLPVVTAAAMQQLMAYDWPGNVRELRNVLNYALVMSDGKQIEVGDLPPEL
                     QMAPDTQSSGAAMSVKPGRQALSETAILQAVTECQGNLTQAAKRLGIGRTTLWRYRRL
                     WQKK"
     gene            2477..3181
                     /locus_tag="VNZ84_01515"
     CDS             2477..3181
                     /locus_tag="VNZ84_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWU65656.1"
                     /translation="MIFQQFFEPESSTYTYLLGCEESRQAVLIDPVDCDVEDYLAALK
                     QHDLTLVYTMETHVHADHVTAADTLRKRLGSKSVVHRDAGATCGDVLVTDGVHLKVGS
                     LEIEVRYTPGHTNGCVSYLVGNRIFTGDALLIDGCGRTDFQQGDAGQLYDSIHAKIFS
                     LPEDTLIYPGHDYQGNTVSTVGHEKRENKRLGGGTSRAEFMNIMANLKLAYPKQIDVA
                     LPANQACGQTVIKQSA"
     gene            3178..4905
                     /gene="sulP"
                     /locus_tag="VNZ84_01520"
     CDS             3178..4905
                     /gene="sulP"
                     /locus_tag="VNZ84_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HWU65657.1"
                     /translation="MSNILLQRFARYLPVLRWGRQYRREQAIHDVMAALTVTLMLVPQ
                     ALAYAILAGLPPQVGLYASLLPLLVYALLGTSSTLAVGPVAVAALMTATAVAPYSAQD
                     PALGLQAALILACLSGIFLSLAGCFKLGFLANFLSHPVMSGFIGGASLVIASSQLPTV
                     LGLHADGSHLLALWRSMLGQWQQIHLPTAIFSALTLGLLLLSRRYGQRLLLSLGCSKF
                     WSQTLVRAIPALLVVIGTIAMHAGWAMFAGVITVGSIPSGLPTLALPHGNLAMWQSLL
                     LPAALISIIGTVESISVAQSLAMKRRERIDPDQELIALGGANLVASLSGGQPVTGGVS
                     RSVVNMDAGAQTPAAGLFTALGMLLATVFLASWLSALPRFILASTIIVAVMSLVDLRM
                     FTDTWRRNRSDFAALLATFMITLLVDIEHGISAGVLLSMGMHLYRSSRPHIAIVGQVP
                     GTEHFRNVARHAVTLCPDVVTLRVDESLYFANARYLEQKVLEVISDNPSLKHVILMCS
                     AVNEVDGSALEVLEAINHHLSALGIGFHLSEVKGPVMDMLQHVPLRQQLNGKIYLTQY
                     QAYAALACH"
     gene            4942..5127
                     /locus_tag="VNZ84_01525"
     CDS             4942..5127
                     /locus_tag="VNZ84_01525"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HWU65658.1"
                     /translation="MRGNISQLERYLRVIIGSGLLMWTLFFEGSAWGWFGIVPLATGL
                     MGWCPLYSLIKPLCHRK"
     gene            complement(5131..5565)
                     /locus_tag="VNZ84_01530"
     CDS             complement(5131..5565)
                     /locus_tag="VNZ84_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65659.1"
                     /translation="MEQKSTDSEHRMLLCGAALWLLMALVLAWCMVGLNFGITPVKAV
                     FAGKFTRLLQAHIDFLLMTALIFGIAASRVRLPWHVRWAMVIGAFTNSSLFLLMSIFP
                     ALDNPELPPPMLFQIYLFASLTTTTYGFGKAAILILKSFLRP"
     gene            5929..6384
                     /locus_tag="VNZ84_01535"
     CDS             5929..6384
                     /locus_tag="VNZ84_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWU65660.1"
                     /translation="MKLNINGKETELNVSDDMPILWAIRDIAGLTGTKFGCGMAQCGA
                     CTVHLDGQAIRSCVTPVSAAVGKKITTIESMQEDKVGQAVQAAWQHIDVVQCGYCQSG
                     QIMSATSLLKQNPKPSDADIDAAMSGNICRCGTYQRIRAAIHEAAANLA"
     gene            6395..8557
                     /locus_tag="VNZ84_01540"
     CDS             6395..8557
                     /locus_tag="VNZ84_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWU65661.1"
                     /translation="MNDMINLSRRSFIKSTALVAGGLVVAFSIPQAKRFMGVANAAEA
                     MPLPAPNAFLRIGADDSITVLLAHSEMGQGVWTTLPMLIADELDAEWSKVKVEHAPAA
                     PAYIHTAYGIQITGGSSTTWSEFDRYRQAGALTRALLLNAAAKQWNVPVAGLRTENGM
                     VINGKQKLRYGQLVGTASQLETPTSVTLKKPEQWKFIGKATKRLDSAEKINGTAKFGQ
                     DVQFDGLKIGMVARAPVFGTKLKSVDDSAARQVPGVIKVVKVPTGVAVIAEHYWAAKQ
                     GREALKLEWDLAGHDKPDSAALLKQYQALAQQPGVQAAKAGDVASASKQAKQTVSAEY
                     VLPYLAHAPMEPLNCAVKISEQGCEIWTGTQMQTTDQASAAKILGLKPEQVKIHTQFL
                     GGGFGRRANPRADFVSEAVEVAKAAGMPVKTVWSREDDIKGGFYRPMFVHRASVSLDK
                     QGKPLAWQHKLVGQSIIKGTPFEGVMIKDGIDATSVEGVADSPYIKGTANHEVHLHSV
                     QSDVPVLWWRSVGHSHSGFVMESLIDEMAHASKQEPLAYRRQLLKDHPRHLAALNLAA
                     DKAGWSKPLPAGVFRGIAVHESFGSYVAEVAEVSVKEGEVKVHRVVVAIDCGLAVNPD
                     GVKAQMESCVAFALGAALSSEVSFKEGQVVQSNFHDYQVLRMKDMPKVEVYIVPSTEK
                     MGGVGEPGVPPLAPAVTNAVFAATGKRIRRLPIGDQLA"
     gene            8562..9545
                     /locus_tag="VNZ84_01545"
     CDS             8562..9545
                     /locus_tag="VNZ84_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWU65662.1"
                     /translation="MQSSDWEVLQRASDWLQQGYGAHLFTVIQTWGSAPRLPGAILVV
                     REDGHLVGSVSGGCIEDDLADKARHQQLPTQPAILEYGIHQDEAQRFGIPCGGQLKIF
                     AEPLTHSTQLAPMLDSLSQRRLLKRSVNLHSGEVRHQHVLPEGLPTLEQDWFHSYFGP
                     QWRLLIIGANQLGSILAAMAQALDFHVLICDPREEMRAEWHVEGADWLPGMPDDVVLD
                     ISPDPHTAIVAVTHDPKLDDMALLEALKSEAFYIGALGSVKNQEKRKQRLRSFDLSEQ
                     EVNRLHGPVGLRIGSRTPAEIAVSILAELIQARSQLQQLGLTQNSASLAAA"
     gene            complement(9567..10166)
                     /locus_tag="VNZ84_01550"
     CDS             complement(9567..10166)
                     /locus_tag="VNZ84_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWU65663.1"
                     /translation="MRPTDSTQRPVVGLLLAGGFSRRFGPQNKLLQPLADGQLVASSA
                     AQTLIQALPDSIAVIRGSESSLTSHLASLGFKIMVCEAQHQQMSDSLKLGIETAQSCF
                     PQMLGLVIALADMPYLQATTIERVAQQLTQTNIVQPMYQGQPGHPVGFGHDLIPELLL
                     VEGDQGARDVLRTHQAEILRFECQDPGILKDIDTPADLL"
     gene            10260..11186
                     /locus_tag="VNZ84_01555"
     CDS             10260..11186
                     /locus_tag="VNZ84_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWU65664.1"
                     /translation="MISPKRLYYSVLLGLLSFPIWVWAQLAVGDYEFAVTINGDKRPY
                     LVHVPMQSKAGQALPMVLVLHGGLGNMQVQANERFYHQVSAAEKYGYIVVFPNGYSRF
                     PGGKFATWNAGNCCGPAVKKQSDDVAVIRAIIQEMQQRANVDAKRIFVDGMSNGGMMS
                     YRLACELSETVSAIASVAGTDNTQDCQPARPVAVLHIHAKDDDHVPFNGGKGQQSMAD
                     VSFNSVAATVQKWVQLNQANAQAERIVEVAGAYCEIHRGGLAPVQLCVTETGGHSWPG
                     GSKPRGGTAGSTAISANDVIWQFFNDYGRRTP"
     gene            complement(11192..11998)
                     /locus_tag="VNZ84_01560"
     CDS             complement(11192..11998)
                     /locus_tag="VNZ84_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HWU65665.1"
                     /translation="MTFNRHFAATGAYLTLFFASLPTFADNRTESRIDRLTETAVPRS
                     QNLYGIGVGVLPRTSGSDEYRALVLPIINANYGDRFFVNALQAGAWLLDSDDQRLRFG
                     ISAEAKFGWDASKGNRTRGMDDRDFSIFVGPLLRWQTDYGTFNMQWTTDASGNSNGQQ
                     FQVQYIKSLIRSPQLRLNGLVGATWNSQKFNNYYFGVHRNEVTANRPFYQAGSGVEWQ
                     LGVNGMMPTIKDHSLLFGAFVTRLSNEQHDSPITETRIQPLAYVGYSIPF"
     gene            12126..12563
                     /locus_tag="VNZ84_01565"
     CDS             12126..12563
                     /locus_tag="VNZ84_01565"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HWU65666.1"
                     /translation="MMHSTQTQLAKILTFSGALPFAGAVLAQFQGMANYHSDYLALTY
                     GAVIISFLAGIHWGLFLTHAQYTRVNLLVSSNLLALIAWASLFFLISVIQSLVQIVCF
                     IALWFIDRRLYAEGVIEGWYYQLRTQITSVVVIFLGALMVLQY"
     gene            complement(12570..12785)
                     /locus_tag="VNZ84_01570"
     CDS             complement(12570..12785)
                     /locus_tag="VNZ84_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65667.1"
                     /translation="MQKYGLAEQDLKKAYALNQHDLDAMLALSRVAFVQKDIPTLESI
                     QPAIQAIDDVQGEQVAKQINSLKQETP"
BASE COUNT         2832 a         3134 c         3637 g         3275 t
ORIGIN      
        1 gcatgaagac agggctgatc aagtggatta acgttcaaca tcccatagct tccgaaaaat
       61 ttaaacaata gaaaacgtat ggtccacttt acgcctttgc agtcgctgat tggcggcttg
      121 ctgattggtt tctcgacatt attgatgata cgcttgctcg gcaaaattgc cgggatcagt
      181 ggcattgttg gccagctctg gtcagccggc ataggcgatc gcgcatggcg gttcgccttt
      241 gtggcggggt tattggtatc accactcctg tatgcgttgt tctctccctt acccgagacc
      301 agtgtggtaa caagtactcc ctggttaatt gtcgctggtt tgctggtggg cttcggtagc
      361 cgcttgggta gtggttgtac gagtgggcat ggtgtgtgtg gactgtcgcg cttgtcatta
      421 cgctccctgg tggcaaccct gacctttatg gcgactgcaa tactggtcgt gtggctgctt
      481 cgtcatgtat ttttgatcgg ttaggggcaa aagatggtta tgttgatcgc gttattagcg
      541 ggcatcattt ttggttttgg gttgattgtg ggcggcatga ccaacccggc taaagtgctg
      601 gcttttctgg atattacggg agattgggat ccctccttgg cttttgtcat gctgggggcc
      661 attgccgtcg gtttttttgc cttcagggcc gccgccagaa aacctgtcag tctgttgggg
      721 atgccggtgc agttgccggg aacccgtctg attgatcgca agttgttatt gggtgccgtg
      781 ctgtttggca ccggttgggg cattgccggt ttttgcccag gtcctgccgt tgcatccgtg
      841 ttgacgggtg gtgtcgctgt ctggctgttt gttgccagca tgctggcagg gatggcgctg
      901 tttacgctgg caacgcgacg cggctggatc taatcagcgc tattttttct gccatagcct
      961 gcggtagcgc cacagtgtgg tgcgaccaat gcctagtcgt ttcgccgcct gggtcaggtt
     1021 gccttggcac tctgtgacgg cttgcaatat ggctgtttct gatagcgcct ggcgaccagg
     1081 ctttactgac atcgcggcac ctgaactttg tgtgtctggg gccatttgta actcaggcgg
     1141 taaatcaccc acttcgatct gtttgccatc tgacattacc aacgcgtaat tcaacacatt
     1201 gcgcagttcg cgtacattgc ccggccagtc ataggccatc agttgttgca tggcggcggc
     1261 cgtgacgacc gggagttgct caccggtaaa ttgttgtttc aggaaatgac gcagtatcaa
     1321 cggaatatct tgcttgcgcg cctgcaagct gggtaagaaa atcggcacca cgcgtaaacg
     1381 gaacagcagg tcctgccgaa aacgcccggc cttgacctct tcacgtaaag ctttgtgggt
     1441 ggcagcgacg atgcgtacat tcgccagcat gggttgactg ccgcctaccg gcaggtattc
     1501 gccggtttcc agcacacgca atagtttagc ctgtaattca tacggcagtt cggcaatttc
     1561 atccaggaac aaggtgccgc cctcagcacg cccaaacagc ccctggtgat cacgaatggc
     1621 accggtgaac gcgcctttca catggccgaa cagttcactt tccagcatgc ccgcattgag
     1681 tgtggcacag ttgatggcaa taaacggggc gccgtgacgt gtgctctcgc catggatggc
     1741 acgcgccacg agttctttgc cggtgccgga gtcaccacgg atcaagacgg gaatgtctgt
     1801 ggccgccaca cgccggatca tgtcaaagat tttctgcatt tctggtgcgt gactgatcaa
     1861 gccatatttt tctcccagag atgattgtcc cgttgatgtg gctgggtctg cttgttgggg
     1921 tagcaggatt tcaagtcccc cggcaaactc gccgttttct ggcagataag ccatggcata
     1981 tttcaatacg gcttgccgtg taccatctac ccggtgcaac agcaagggtg caccttctac
     2041 cttgccggat tcggtcagcc cgcaaccgcg catgcattgt tcacatcggt tggccgtcag
     2101 gcagttttcg cccagcagtg aaggctgggt gaagccgagc agtttttcgg cgccagggct
     2161 ccagtagttg atgcgcttat gtgcatcaac ggtaaacacg gccgcgtctg gcatgatgag
     2221 tgaaatcatg cgcgccagtg attccccctg ggcgcgggtc aagctctcgg actgactaaa
     2281 aaaatcgggt gtggtttgtt ccatttttta attgaaacgt ttcagttgat gttttgttgt
     2341 ttcattgaaa cattttggtg ggatgattgc aagcatttat tttttataag tcattgattt
     2401 ttatgttttt agatccaatt tgaaacattg gcacccgttt tgctgaagca tcgttatctt
     2461 gataaggaac ctggtcatga tatttcaaca attttttgaa cctgaaagta gcacctatac
     2521 ctatctgcta ggttgcgaag aaagcaggca agctgtgctg atcgatcccg tagactgcga
     2581 tgtagaggat tatctggctg ccttgaagca acatgatctg acactggtat atacgatgga
     2641 gacgcatgtc catgctgacc atgtgacggc agccgatacc ttgcgcaaac gtttgggtag
     2701 caagagtgtg gtacatcggg atgcgggcgc cacgtgtggg gatgtgctgg tgacagatgg
     2761 tgtgcatttg aaagtcggct cgctggagat agaggtacgc tatacaccag ggcacaccaa
     2821 tggttgcgtc agttacctgg ttggtaaccg catctttacc ggcgatgcct tgctcattga
     2881 tggttgtggc cgcaccgact tccagcaagg ggatgccggg cagttgtatg acagcattca
     2941 tgccaaaatt ttcagcttgc ctgaggatac gctgatctat cctggccatg attatcaggg
     3001 caataccgtc tctaccgtgg gacatgaaaa gcgtgaaaac aagcgcctgg gtggcgggac
     3061 ttcccgtgca gaatttatga atatcatggc taacctcaag ctcgcgtatc ccaagcaaat
     3121 tgatgttgcc ctgcctgcta atcaggcttg tgggcaaacg gtgatcaagc agtccgcatg
     3181 agtaacattc tattgcagcg attcgcccgg tatctgccgg tgctgcgctg ggggcggcag
     3241 taccggcgtg agcaggccat acatgatgta atggcggcac tcacggtcac cttgatgctg
     3301 gtgccgcagg cgttggccta tgccattctg gcaggtttgc caccccaggt tgggctctat
     3361 gccagcctgt tacccttgct tgtatatgcg ttgttgggga ccagttcaac gctggcggtg
     3421 ggcccggtag ccgtggccgc cctgatgacc gcaacggcag tggcgcccta ttcggcacag
     3481 gatccggccc ttggtctaca agcggcgctg atcctcgcct gtctgtctgg aatttttctc
     3541 tcattggccg ggtgctttaa attaggcttt ctggcgaatt ttttatcaca tcccgtcatg
     3601 agcggcttta tcggtggtgc aagcctggtg attgccagca gtcaattgcc aacggtgctc
     3661 ggtttacacg cggatggtag tcatctcctg gcgctttggc gtagcatgct aggtcagtgg
     3721 cagcagatcc atttgccaac agctattttt tcggcgctga ccctgggttt gcttctgttg
     3781 agcaggcgct acgggcaacg gttgcttttg agcctggggt gttccaagtt ttggtcgcag
     3841 accttggtgc gggccattcc ggccttgttg gtggtcatag gcactattgc catgcacgca
     3901 ggctgggcaa tgtttgcagg tgtgataact gtcggttcca ttccatccgg tttacccacc
     3961 ctggccttac cccatggcaa tctcgccatg tggcagtctt tactgctgcc agctgcactg
     4021 atttccatca tcgggacggt ggagtccatt tctgtcgcgc aaagtctggc catgaaacgg
     4081 cgtgagcgta tagatccaga ccaggagctg atcgccttgg gtggggcgaa tcttgttgct
     4141 agcctgagtg gtgggcagcc agtcaccggt ggcgtctccc gttcggtggt caacatggat
     4201 gccggggcac aaacaccggc agcagggttg tttacggcgt tggggatgtt gctggcaacc
     4261 gtgtttttgg cctcgtggct cagtgccttg cccaggttta ttctggccag cacgattatt
     4321 gttgcagtga tgagcctggt agatttgcgg atgtttaccg atacctggcg gcgcaaccgt
     4381 agtgattttg ccgctttgct ggctaccttt atgatcactt tactggtgga tattgagcat
     4441 gggatcagtg caggtgtgct gctatccatg ggcatgcatc tttaccgtag cagccgccca
     4501 cacattgcca ttgtcgggca ggtgccgggt accgagcact ttagaaatgt cgcacgtcat
     4561 gcggtgaccc tttgtccgga tgtggtgacc ttacgggtgg atgagagcct gtattttgcc
     4621 aatgcacgtt atcttgagca aaaagtactc gaagtgatct ctgacaaccc ttccctcaag
     4681 catgtgattc tcatgtgctc agctgtcaac gaagtggatg gcagtgcgct tgaagtgctt
     4741 gaagcgatta atcatcattt gtccgcgctg ggaattggtt ttcatttgtc cgaggtcaag
     4801 ggccccgtca tggatatgtt gcaacatgtt cccctgaggc agcagctcaa tggcaagatt
     4861 tatttaaccc aatatcaagc ctatgcagcg ctggcctgtc attagtttga agtatttccg
     4921 ggcaacaaga aggaggcggt gatgagaggg aatatcagtc agttagagcg ttatttaaga
     4981 gtcattattg gctcaggttt gctgatgtgg acgctgtttt ttgaagggtc ggcatggggc
     5041 tggtttggca ttgtgccgtt ggcgactggg ctgatgggct ggtgtccgtt gtattcgctc
     5101 attaagcccc tttgtcaccg aaaataagga ctaaggcctt aggaaagatt ttagaatcag
     5161 tatggcggcc ttgccaaagc cataggtcgt cgttgtcagg ctggcgaaca gataaatttg
     5221 gaacagcata ggtggcggta actccggatt atccagcgcc gggaaaattg acatcaacaa
     5281 aaacaggctg gaattggtga atgccccaat caccatcgcc cagcgtacat gccagggtaa
     5341 gcgcacgcga ctggcagcaa tgccaaaaat caatgcggtc atcaacaaga aatcaatatg
     5401 tgcttgcaat aagcgggtaa attttccggc aaacacggct ttgaccgggg tgatcccaaa
     5461 gtttaatccc accatacacc aggccagtac caaggccatc aataaccata atgccgcgcc
     5521 acacagcaac atacggtgtt cactgtcggt tgatttttgt tccatatttt gctccttgtt
     5581 gaatgctgtc tagcataagt gctgggtgtt ttttagcctg actggaacgg agttcgggca
     5641 tgtctgggac ggagatgagt tatcttcagg agatcttccc tgatccttaa atacgagtat
     5701 attgcgccat tgaacaaggc taagtggcgc tgccccaagt gcagaacaat ctgttgacac
     5761 tagggtcgaa tgaccattca ttttgtgtgg taatcacatt aaattttagt tttatgattt
     5821 aggggtgcac atacatacag ttgtgtatgt tagtatattt ttattccagc caacgttaga
     5881 ttggtttttg agaataggca caacaaattt tcacaaggaa cccgccctat gaaacttaat
     5941 atcaatggca aggaaaccga acttaatgtc agtgatgaca tgcccatctt atgggcgatc
     6001 cgcgatattg caggactgac gggtaccaag tttggttgtg gtatggccca atgtggcgcc
     6061 tgtaccgtgc acctggatgg tcaggccatc cgttcgtgcg taacaccagt cagtgcggcg
     6121 gtgggtaaaa aaatcaccac cattgaatcc atgcaggaag acaaagttgg tcaggccgta
     6181 caggctgcct ggcaacatat tgatgtggtt cagtgcggtt attgccaatc tggccagatc
     6241 atgtcggcca cctcattact taagcagaat cccaaaccca gcgatgccga tattgatgcc
     6301 gccatgagtg gcaatatctg tcgttgtggg acctatcaac ggatacgtgc agccattcat
     6361 gaagctgcgg ctaacctggc ttaaggagag aacgatgaac gatatgatta atctctcacg
     6421 tcgcagtttt attaaaagca cggcgctggt tgcaggcggc ctggtcgtcg cattcagcat
     6481 cccgcaagcc aagcggttta tgggtgtggc aaatgccgca gaggcgatgc cactgccagc
     6541 gccaaatgct tttttgcgca taggcgcgga tgacagtatc actgttttgc tggcccacag
     6601 tgaaatgggg cagggcgtgt ggaccacttt gccgatgtta atcgcagatg agctggatgc
     6661 tgagtggagc aaggtgaagg ttgagcatgc tcccgcagcg cctgcctata ttcacaccgc
     6721 gtatggcatc cagattacgg gtggctcatc caccacctgg tcggaatttg accgttatcg
     6781 ccaggctgga gccctgacac gcgccttgct attgaacgct gcggccaaac agtggaacgt
     6841 gcctgtggcg ggtttgcgta ccgaaaatgg catggtcatt aacggtaagc agaagttacg
     6901 ctatggccaa ctggtgggaa cggccagcca gctggaaaca ccaaccagtg tcaccctgaa
     6961 aaaacccgaa caatggaagt ttattggtaa ggccaccaag cgcctggata gtgcagaaaa
     7021 aatcaatggc acggccaagt tcggccagga tgtgcagttt gatggactga aaattggcat
     7081 ggtggcacgt gcgccagtct ttgggaccaa gctgaaaagt gtagatgaca gtgctgcgcg
     7141 ccaagtgccg ggtgtcatca aggtcgtcaa ggtgccgact ggggtggccg tgatcgctga
     7201 gcattattgg gcggccaagc agggccgcga agcgcttaag ctggagtggg acctggccgg
     7261 acacgataaa ccggattcag ccgctttgtt gaaacagtac caggccctgg cgcaacagcc
     7321 tggcgtgcag gcagccaaag ctggcgatgt cgccagcgcc agcaaacagg ccaaacagac
     7381 ggtgagtgca gagtatgtat tgccatacct ggcacatgca ccgatggaac ccttgaattg
     7441 cgccgtgaaa atctctgaac agggatgtga aatctggact gggacgcaaa tgcagaccac
     7501 cgatcaggca tctgccgcca agatactggg gctgaaacct gagcaggtaa aaatacatac
     7561 gcaatttctc ggtggcggtt ttggccgccg tgccaatcca cgggccgatt ttgtttctga
     7621 agcggttgaa gtggccaaag cggccggaat gccagttaaa acagtgtgga gccgtgaaga
     7681 tgacatcaag ggtggttttt accggccgat gtttgtgcac cgtgctagcg tctcgctgga
     7741 caagcaaggc aaaccactgg cctggcagca taagctggtt gggcaatcca tcattaaagg
     7801 cactccgttt gagggtgtca tgatcaagga cggcattgat gcgacctcgg tagagggcgt
     7861 ggcagactct ccctatatta aaggcacggc caaccatgag gtgcacttgc attcggtaca
     7921 aagtgatgtg ccagtattgt ggtggcgatc agtgggacac agccattcag ggtttgtcat
     7981 ggaaagcctg attgacgaaa tggcccatgc cagcaaacag gaaccattgg catacagacg
     8041 ccagctactg aaagaccatc cacgtcatct ggcggcgctt aacttggctg ccgacaaggc
     8101 cggatggagc aagccgttac ctgcgggtgt attccgcggg attgcggtgc atgagtcttt
     8161 tggtagttat gtggctgaag tcgctgaagt gtcggtgaaa gaaggcgaag tcaaggtaca
     8221 tcgtgtggtg gtggccattg attgcggact cgccgttaac ccagatggtg tcaaagcaca
     8281 aatggaatcc tgcgtggctt ttgcgctggg tgcggcactg agtagtgaag tcagttttaa
     8341 agaaggccag gtagtgcaat ccaacttcca tgattatcag gtgttacgca tgaaagatat
     8401 gcccaaggtg gaagtgtata tcgtgcccag taccgaaaaa atggggggcg tcggtgagcc
     8461 tggcgtaccg ccactggcgc cggcagtcac caatgcggta ttcgcggcca ctggcaaacg
     8521 tattcgccgt ttgccgatag gcgaccaact ggcgtagggc tatgcaatcg tcagattggg
     8581 aagtgctgca acgcgccagc gactggctgc aacaagggta tggtgcgcac ctgttcactg
     8641 tgatccagac ctggggctcg gcaccgcgtt tgccgggcgc cattctggtg gtgcgtgagg
     8701 atggccatct ggttggctcg gtgtccggcg gttgtattga ggacgacctc gctgataaag
     8761 caaggcacca acaattgcct actcagcctg ccattcttga atacggcatc caccaggacg
     8821 aggcgcagcg ttttggcatt ccgtgtggcg ggcaactaaa aatttttgcc gaaccactga
     8881 cccattccac acaactggca ccgatgttgg acagtttgtc gcaacggcgc ttattgaaac
     8941 gttcagtcaa cctgcacagt ggcgaagtgc gccatcagca cgtgctgcca gaaggcttgc
     9001 cgactctgga gcaagattgg tttcacagct actttggccc gcaatggcgc ttgctcatta
     9061 tcggtgccaa ccagctgggc tctatattgg ctgccatggc gcaagcgctg gattttcatg
     9121 tcttgatctg cgatccacgt gaggaaatgc gtgcagaatg gcatgtggaa ggcgcggact
     9181 ggttgcccgg catgccggat gatgtagtac tcgatatttc acctgatcca catactgcca
     9241 ttgtcgccgt gacacatgac cccaagcttg atgatatggc cttgcttgag gcattgaagt
     9301 ctgaggcgtt ttatatcggc gcgttaggct cggttaaaaa ccaggagaaa cgcaagcagc
     9361 gcctgcgcag ttttgacctc agcgagcaag aggtcaaccg cctgcatggt ccggtaggct
     9421 tgcgtattgg gagccgtacg cctgccgaaa tcgcagtgtc tattctggct gagctgattc
     9481 aggcgcggtc acaactgcaa cagcttgggc tgacacaaaa cagtgccagt ctcgcagcgg
     9541 cttgacctgc atagatcggc atctggtcat aacaggtctg cgggcgtatc aatatccttt
     9601 aagatgccag gatcctggca ttcgaatcgt aaaatctcag cctgatgcgt acgcaagacg
     9661 tcacgcgcac cttgatcccc ttcaaccaat agtaattccg gaatcaggtc gtgaccaaag
     9721 ccaacaggat ggccgggctg gccttggtac atcggttgta caatattggt ctgcgtgagt
     9781 tgctgtgcga cgcgttcaat cgtggttgcc tgaagataag gcatatctgc cagcgcaatg
     9841 accagcccca acatctgggg aaagcaggat tgcgcagtct caatgcctaa tttcaggctg
     9901 tctgacatct gctgatgctg cgcttcgcat accataatct tgaaaccgag tgacgcaaga
     9961 tggctagtga gtgatgactc actgccacgt atcaccgcaa tgctgtccgg caacgcctgg
    10021 atcagggtct gtgcggcact tgacgccacc agttgcccat ccgctagtgg ctgtagcaat
    10081 ttattctgag ggccaaaacg tcgcgaaaat ccacctgcca gcaataagcc gacaacaggt
    10141 cgttgtgtgg agtcagtagg gcgcatattt ctgtgcttgc aagggtttaa aggtggtcaa
    10201 caactcactt cactggcgcg ttcatgtatg ctcttgtcat cctcttgtct ggagcacgta
    10261 tgatttcacc taaacgttta tattacagtg tgttactggg tttattaagt tttcctatat
    10321 gggtatgggc acagcttgcg gttggcgatt atgagtttgc cgtgaccatc aatggtgaca
    10381 agcgccctta tctggttcat gtgcccatgc agtcgaaagc tggccaggcc ttgcccatgg
    10441 tgctggtatt gcatggcggc ctgggtaata tgcaggtaca ggccaatgag cgcttttacc
    10501 accaggtttc ggccgcagaa aaatatggct acattgtggt gttccccaat ggttacagcc
    10561 gtttcccagg tggcaagttt gccacctgga acgcgggcaa ttgttgtggt cctgccgtca
    10621 aaaaacaaag tgatgatgtt gccgtgatcc gcgccatcat ccaggaaatg cagcaacgcg
    10681 ccaatgtgga tgccaagcgc atttttgtcg atggcatgtc caatggcggc atgatgagct
    10741 atcgcttagc ttgcgaactc tctgagaccg taagcgcaat cgcttcggtg gcaggtaccg
    10801 acaatacaca agattgccaa cctgccaggc cggtcgccgt gctacatatt catgctaaag
    10861 atgacgacca tgtgccgttt aatggtggca aaggccagca atcaatggcg gatgtgagtt
    10921 tcaattcggt ggcggcgacg gtgcaaaaat gggtgcagct caaccaggca aatgcacaag
    10981 ccgaacgtat tgtagaggtc gcaggcgctt attgcgagat tcatcgtggc ggactggcac
    11041 cagtgcaact ctgtgtcacc gagacaggtg gccactcctg gcccggcggc agtaagccac
    11101 gaggtgggac tgccggatcc acggccattt ctgccaatga tgtgatctgg cagtttttta
    11161 atgactatgg ccgccggacg ccctaatgcc tctaaaaagg aatgctgtaa cctacgtatg
    11221 ccaagggttg tattcgggtt tcggtaatcg ggctgtcatg ctgctcattg ctaagtcgtg
    11281 tgacaaaggc accaaacagc aaagagtgat ctttaattgt gggcatcatg ccattgacgc
    11341 cgagctgcca ctcaactcct gaacctgcct ggtaaaaagg ccggttcgct gtgacttcgt
    11401 tgcgatgcac accaaagtaa taattattga atttctggct gttccaggtc gccccgacca
    11461 acccattcag gcgtaattgt ggtgaccgga tcaggctctt gatatattgc acctggaact
    11521 gctggccgtt gctgttgccg ctggcgtcag tcgtccattg catattaaag gtgccatagt
    11581 cagtttgcca acgcagcagt gggccgacaa agattgaaaa gtccctgtcg tccatgccgc
    11641 gggtgcgatt gcctttgctg gcatcccaac caaattttgc ttccgccgaa ataccaaacc
    11701 gcaaacgctg gtcatcacta tctagcaacc atgcaccggc ctgtagtgcg ttaacgaaaa
    11761 atcggtctcc gtaattagca ttaataatcg gcaacacaag cgcccggtat tcgtcggaac
    11821 cagatgttct gggcagtacg ccgaccccaa taccataaag gttttgtgag cgcggcacgg
    11881 cggtttctgt cagtcggtca atgcgggatt cggtgcggtt gtcggcaaag gtgggcaggc
    11941 tcgcaaagaa aagtgtcagg taggcacctg ttgctgcaaa atgtcggttg aaagtcatgt
    12001 gtgtatccat tgttgtaata atatagtttg gtagcttgag cggatgagct gtcatttagt
    12061 tccagctgca tgcagcaaaa ccaaaatgac tggcgatggt cacatcgcag gcttaaggag
    12121 agcttatgat gcattctacc cagacccaac ttgcgaaaat actgacattc tcgggagccc
    12181 tgccgtttgc cggtgcggtt ctggcgcaat tccaagggat ggccaactat catagcgatt
    12241 atctcgcact gacatatggt gcagtgatca tttctttttt ggctggcatt cattggggat
    12301 tgtttttaac ccatgcccaa tatacccggg tcaatctact ggtaagcagt aatctgcttg
    12361 cgctgatcgc atgggcctct ttgttttttc tgatttcggt gattcagtcg ctggtacaga
    12421 ttgtttgctt tatcgccttg tggtttattg atcgcaggct gtatgccgag ggcgtgattg
    12481 aaggttggta ttaccagcta cgcactcaga tcacttcggt ggttgtgatt tttctgggtg
    12541 cgttgatggt tttacagtat taaagccagt tacggcgttt cttgtttgag cgagttgatt
    12601 tgcttggcga cctgctcccc ctgtacgtca tcgatggcct ggatagcggg ttgaatagac
    12661 tcaagggtgg gaatatcctt ttgcacaaat gcgacgcgag acagcgccag catcgcatcc
    12721 agatcatgct gattgagggc ataagccttt ttcagatcct gttcggctag accatacttt
    12781 tgcaatcctt cttccgcgag acccaggaag taccaggcat ctgcagaggc cggttcttta
    12841 accagccatt gttaggcgat acttttgagc gctgccca
//
LOCUS       DATMSK010000047        15887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_380155,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000047 DATMSK010000000
VERSION     DATMSK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 15887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15887
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380155"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1110)
                     /locus_tag="VNZ84_01575"
     CDS             complement(187..1110)
                     /locus_tag="VNZ84_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU65668.1"
                     /translation="MDTLRSIESFVKAVQAGSIAAGARLQGITAAAASQNIQRLEKSL
                     GTRLLIRTTRKLAMTESGELYYAEVQHTIEALARAQSVITEFHGQPQGRLRIGSSVAF
                     GRHVLMPLIPTFTRNFPKVSIELVLSDHSLDHVTEDIDISIRFKQQLEPGLVARKIAT
                     VPVLFCAAPAYLQRKGIPQAPEALSEHDCLLFRIPVDGRLLSWTFNRNGMLYEPEIKP
                     SIICNDIDSLHRLAKEGAGIARLAAFVANEDIAKGLLIPLFQPSSTYPDMMVAESLPL
                     EFYACFRDKHAITNKVRAFMDYLVSEMPSSW"
     gene            1227..1481
                     /locus_tag="VNZ84_01580"
     CDS             1227..1481
                     /locus_tag="VNZ84_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HWU65669.1"
                     /translation="MNQTKNSSRKIPKRFTPYVFAFFMAGIMAFLMSMVIVAANSGLH
                     AGYVLRVLNAYALAMPVAFCCVLIVRPIVMHLVTRLVDGH"
     gene            complement(1539..2072)
                     /locus_tag="VNZ84_01585"
     CDS             complement(1539..2072)
                     /locus_tag="VNZ84_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWU65670.1"
                     /translation="MLKLQQTLGWVQSVDSRLCVKVSHTGQYRFIRSLFRAISRMGDG
                     MFWYLLMLMIAVTQGQQGLYVCLHMLAAGLTGTLVYKWLKHRTSRPRPFQVRQDVLLS
                     GTPLDYFSFPSGHTLHAVAFGMVAVHYYPQLFPIVYPFVVLVGMSRVVLGLHYPSDVL
                     AGAAIGYLIVMLYAPLF"
     gene            complement(2182..2463)
                     /locus_tag="VNZ84_01590"
     CDS             complement(2182..2463)
                     /locus_tag="VNZ84_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HWU65671.1"
                     /translation="MAIKLLFLLLMLFMANLNLVSDKWLGIAGSLTQLWQRFVALLPA
                     YFITLGIAYLVERFVMGQVWPQGWEYYSITLCVFLVLSFPGFIYRVLWK"
     gene            complement(2468..3913)
                     /gene="nuoN"
                     /locus_tag="VNZ84_01595"
     CDS             complement(2468..3913)
                     /gene="nuoN"
                     /locus_tag="VNZ84_01595"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HWU65672.1"
                     /translation="MDNMQFDLLALLPELVVLGMAMFILLLDLFILPQNRFMIYGLSQ
                     FTLFAAAFFTFKTHTPAVGFAFSHMFIDDTLADVIKMMMYLGTSLILVYTRKYLQDRN
                     LYRGEFYAMVLFGLLGMMIMVSGHNLLTIYIGLELLSLCLYSLVAFDRDNPKASESAM
                     KYFVLGALASGMLLYGMSMLYGMTGSLDVSDIANNIVQQPKSPVLIMGLVFVVAGIAF
                     KFGAVPFQMWVPDVYQGSPTPMTLLIGSVPKLAAYAMTVRLLVQGLHPLALDWQDMLV
                     LMAVLSIIIGNFSAIVQTNLKRMLAYSTISHVGFIMFGMMSANANGFASSFFYISAYV
                     LMSIAGFGIILLLSRQGFEAEEINDLKGLNQRHPWIAFLMLVIMFSMAGIPPTIGFYA
                     KFTVLQAAWQAGFTWQVVLAVLMATIGAFYYLNIVRKMYFDAPEDHTPLTAPVDMRFI
                     LSVHALALLGLGLFPEILLSVCGHSLLISLQ"
     gene            complement(3929..5437)
                     /locus_tag="VNZ84_01600"
     CDS             complement(3929..5437)
                     /locus_tag="VNZ84_01600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWU65673.1"
                     /translation="MMQADLTSISWLSLAVWLPVLSGVLVLVLGGDHKATLTRWLALA
                     GSLVSFLVTVPLYTQFDVQDGFFQFEELLPWVPAFNMHYHLGIDGFSMPLVLLTSFTT
                     VIVVLAGWEVITKHVAQYMAAFLIMSGIMIGVFTALDAILYYVFWEAMLIPMFLVIGI
                     WGGPNRVYATIKFFLYTLLGSLLMLVAFIYLYHQTGSFELADYYLLPLSMQAQIYIFI
                     AFFMAFAVKIPMWPVHTWLPDAHVEAPTGGSVVLAAIALKLGGYSFLRFAMPIAPDAA
                     HYFSQAMIALSLIAVVYIALVALVQKDMKKLIAYSSISHMGFVTLGFFMFGQLALEGA
                     MVQMISHGFISSAMFLSVGVLYDRVHSREISAYGGVVNKMPVFAAFAVLFAMANSGLP
                     GTSGFVGEFMVILAAVKFNFWVAFFAATTLIFGAAYTLWMTKRVFFGNVTNHHVAELS
                     DLNKREFLILGILAVLVIGFGVYPEPLTEVMQATSTEFLKHMAISKLPVTGY"
     gene            complement(5437..7512)
                     /gene="nuoL"
                     /locus_tag="VNZ84_01605"
     CDS             complement(5437..7512)
                     /gene="nuoL"
                     /locus_tag="VNZ84_01605"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWU65674.1"
                     /translation="MTIKQILILIPLLPLMAAALVGVFRNFLPRWAAHVLTIAGVGAA
                     FALSAYIFNQTLSGFTMNEAVYTWLNSGDVHFEVGFLIDNLSAMMMVVVTFVSLMVHI
                     YTIGYMAEDPGYSRFFSYISLFTFSMLMLVMSNNFMQLFFGWEAVGLVSYLLIGFWFT
                     RPTAIYANLKAFLVNRVGDFGFLLGIGMVLYYFGTLDYATVFQRAPELANTQVNLLGS
                     CNWSLMTVTCLLLFVGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVA
                     RMSPLYELSTTALSTVMVIGAITALFMGFLGIIQNDIKRVVAYSTLSQLGYMTVALGA
                     SAYSVAIFHLMTHAFFKALLFLGAGSVIMGMHHDQDIRNMGNLKKYMPVTWITSLIGS
                     LALIGTPFFAGFYSKDSIIEAVELSHIPGSGFAYFAVIVGVFVTAFYSFRLYFLVFHG
                     EEKWRHAKHDGHAHAAHADAHHDDAHHDEDHAHDEEHHHGLSPNDNPHEPGWVVTLPL
                     ALLAIPSLVIGYIALEPMLYGEFFKNVIFVNPEAHPAMEHLAHHYHEFMHSPAGMAIH
                     GFTSLPFFLAASGVLLSWFFYMKRPDIPAAIKVRFIVIYNILENKYGFDSFNEKFFAG
                     GSRCLGSLLWKFGDITLIDGAMVNGTANTMGKIAAKIRHLQSGLIYHYAFAMIIGVFL
                     LMTFFIKIN"
     gene            complement(7518..7823)
                     /gene="nuoK"
                     /locus_tag="VNZ84_01610"
     CDS             complement(7518..7823)
                     /gene="nuoK"
                     /locus_tag="VNZ84_01610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWU65675.1"
                     /translation="MVGLSHYLILGALMFAISVIGIFLNRKNVIILLMAIELMLLAVN
                     LNFIAFSHYLNDIAGQVFVFFILTVAAAESAIGLAILVVLFRNLRTINVDDLDQLKG"
     gene            complement(7826..8455)
                     /locus_tag="VNZ84_01615"
     CDS             complement(7826..8455)
                     /locus_tag="VNZ84_01615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWU65676.1"
                     /translation="MLFTDIVFYVLAAILLFSGIRVITTRNPVHAALFLVLAFFTAAG
                     IWLLLEAEFLAIALILVYVGAVMVLFLFVVMMLDINLDKLREGFWEYLPMAGLIGVLM
                     VVEMTMVLAEKNLHPSEAVELPANYSNTAALGKVLYTDYLLPFELAAVVLLVAMIAAI
                     VLTLRERKDNKSMNPAEQVKVKRNDRLRIVSMPAEIEEAPAAAAAEEKK"
     gene            complement(8471..8962)
                     /gene="nuoI"
                     /locus_tag="VNZ84_01620"
     CDS             complement(8471..8962)
                     /gene="nuoI"
                     /locus_tag="VNZ84_01620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWU65677.1"
                     /translation="MFNSIKSTLGSLMLWEMLKGMALTGRYFFAKKITIQYPEERTPQ
                     SNRFRGLHALRRYPNGEERCIACKLCEAVCPAMAITIESDQREDGTRRTTRYDIDLTK
                     CIFCGFCEESCPVDSIVETRIFDYHGEQRGDLLYNKQMLLEVGDKYEAQIAADRAQDK
                     VYR"
     gene            complement(9001..10029)
                     /gene="nuoH"
                     /locus_tag="VNZ84_01625"
     CDS             complement(9001..10029)
                     /gene="nuoH"
                     /locus_tag="VNZ84_01625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWU65678.1"
                     /translation="MQFFAELFGSYWADVQLVVWTLLKIVAIVLPLMIGVAYLTLAER
                     KVIGYMQVRIGPNRVGYFGLLQPLADGLKLLFKEIILPTASNKALFFIGPVLAIAPAF
                     AAWAVVPFDATLVLANIDAGLLYILAMTSVAVYGVIIAGWASNSKYAFLGSLRSAAQI
                     VSYEIAMGFTLVGVLMCANSLNLGKIVMGQEGGFWHWYFLPLFPLFVVYFISAVAETN
                     RAPFDVAEGESEIVAGFHVEYSGMAFAVFFLAEYANMMLVSMLAALMFLGGWLSPVPF
                     LPDSILWLLAKVAFLLFLFLWFRATFPRYRYDQIMRLGWKVFIPITLVWIIVVGAMMQ
                     TQWAYLFH"
     gene            complement(10031..12463)
                     /gene="nuoG"
                     /locus_tag="VNZ84_01630"
     CDS             complement(10031..12463)
                     /gene="nuoG"
                     /locus_tag="VNZ84_01630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HWU65679.1"
                     /translation="MLNIEIDGKPLEVEHGSTIIDAADAAGIAIPRFCYHKKLSVAAN
                     CRMCLVQVEKFNKPLPACATPVADGMKIYTRSKSAVEAQQSVMEFLLINHPLDCPICD
                     QGGECDLQDIAVAYGASGSRYTEEKRVVFNKNIGPLVSTDMTRCIQCTRCVRFLKEVG
                     GMQELGLVNRGEHAEITAYVDQSVNSELSGNIIDLCPVGALTSKPFRYSARSWELVRR
                     PSIAPHDGLGSHIEVHVKDNKVMRVLPREKESINECWLSDRDRFSYEGLNSPDRLKVP
                     MIKHNGQWQETDWKTALEFAAGQLKDITKEYGGDALGVLVSPNSTMEEGYLIKQLAEG
                     LGCGNVDYRLRQTDFRLDGKRTGTPWMGCNIQEIEELDRILLIGSNLRNEHPLLAQRI
                     RKAVANGAELCIVSPLDNDPLMDIAHKVIVRPNDMVNVLGQILKAMSGLQKLSLCLPP
                     ALNKLLEEIQVRPNTQKIAECLAGISEELILIAPKVGIFLGNMALSDPRFTEMYSMAE
                     AIGGITGAKGGILPPAANSTGMHLMGVMPSATGMHARAMLEVPRKAYLLLNIEPELDC
                     QHAALASAAMAKAECVIALTAYKSSALENADILLPITPFTETSGTFMSMEGRVQSFQA
                     VTRPLGEARPGWKVLRVLANTLGLSGFEYNSSEEVKNAIFNGEKPSAVVWRSLNNNLK
                     ELVEIQVNIKKEGLQRIGEVPQYEFDPIVRRSAPLQKTKYNIRPMARMRAEQLAELGL
                     ADGDIVVARQDKGSAVLKVRLDNHVAKGCVRVAAAHPLTAGLGDLMGDITLEKASAEQ
                     VAIYEKQLTEMA"
     gene            complement(12490..13824)
                     /gene="nuoF"
                     /locus_tag="VNZ84_01635"
     CDS             complement(12490..13824)
                     /gene="nuoF"
                     /locus_tag="VNZ84_01635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWU65680.1"
                     /translation="MAKTSNAIVSSKPVVKTDLAGQTLVTLRTLTEANPASLATYEKL
                     GGYAQLKRLVTEKIKAPDIIGQVKVSNLRGRGGAGFPTGLKWSFMPRYYDGTKYLVCN
                     TDEGEPGTFKDRDIIRYNPHQLIEGMAIAAYTLGARVGYNYIHGEIWQDYEIFEAALA
                     EAREAGYLGENLFGTEFSFDLYAVHGYGAYICGEETALIESIEGKKGQPRFKPPFPAS
                     YGVFGKPTNVNNTESYASIPWIMQHGGQAFADLGVPNSGGSKLFSVSGHVEKPGNYEV
                     KMGTPFKELLEMAGGIWKGRQLKAVIPGGPSTAVMPASAIENATMDYDGLSKAGSSLG
                     AGSMIVMDETTCMVKTLKRLSYFFYEESCGQCTPCREGTGWVYRIIERIVDGKGKMED
                     LDLLTDLSNNISGRTICALGDAAATPVLSFIKHFRPEFEYYIQHGKSMVDGK"
     gene            complement(13835..14314)
                     /gene="nuoE"
                     /locus_tag="VNZ84_01640"
     CDS             complement(13835..14314)
                     /gene="nuoE"
                     /locus_tag="VNZ84_01640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HWU65681.1"
                     /translation="MLSAESVEKIEYELSKYPADRRQAAVMSALRIVQMERGWLSKES
                     ISEVAKYLRIPEIAALEVATFYNMYDLEPVGQHKITICTNISCMLRGSDEIVEHLQHK
                     LGVGFNEVTPDGKFCLKEGECMGCCGGAPLLHVNNSEMHEFLTTEKVDALINELSKG"
     gene            complement(14375..15628)
                     /locus_tag="VNZ84_01645"
     CDS             complement(14375..15628)
                     /locus_tag="VNZ84_01645"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWU65682.1"
                     /translation="MAEIKNYTMNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAENRTYLQSVPYMDRLDYVSMMANEHAYVMAIEKMLGIDVPVRAQYIRVMFD
                     EITRILNHLLWLGAHALDVGAMTVFLYAFREREDLFDVYEAVSGARMHAAYYRPGGVY
                     RDLPEKMPQHETTKFRSADEIKKLNENRQGSMLDFIEDFTNRFPTYVDEYETLLTDNR
                     IWKQRTVGIGVVTPERALALGLTGAMLRGSGIAWDLRKKQPYEVYDKMDFDIPIGVNG
                     DCYDRYLVRVEEMRQSNRIVKQCIDWLRKNPGAVISDDNKIAPPNRENMKTNMEDLIH
                     HFKLFTEGFHVPAGEAYAAVEHPKGEFGIYMVSDGANKPYRLKIRAPGFAHLAALDEM
                     TRGHMIADLVAIIGTQDIVFGEIDR"
     gene            complement(15621..>15887)
                     /locus_tag="VNZ84_01650"
     CDS             complement(15621..>15887)
                     /locus_tag="VNZ84_01650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWU65683.1"
                     /translation="VDIWPVANWFEREAFDLYGIMFENHPDLRRLLTDYGFVGHPFRK
                     DFPMIGNVEMRYDPVQQRVIYQPVSVELRNNVPRVIRDEGAHHG"
BASE COUNT         4434 a         4211 c         3602 g         3640 t
ORIGIN      
        1 ataaaaacga ggatcaagat ggcgagaagt aacgatagaa ccaaaagata gaaccggaga
       61 acaacaacac tacttgaacg tcgtgagggt gtactatgca cgcaatattg aatattggca
      121 acaagtattt gataaatcgg cataaattga aggagtcatt gcatcccaca caacaataca
      181 taccaactac caagaacttg gcatttcact cacgagataa tccataaatg ccctgacctt
      241 attggtaatt gcatgcttgt ctctaaagca agcataaaac tccaagggca agctctcggc
      301 gaccatcata tctggatatg tcgacgatgg ctggaatagc gggatgagca acccttttgc
      361 aatatcctca tttgcaacaa atgccgccag gcgcgcaatc cctgccccct ctttagccag
      421 gcgatgtaac gagtcgatat cgttgcaaat aatactcggc ttgatttcag gttcatacag
      481 cataccattg cggttaaaag tccagctaag caagcgccca tcaacaggga tgcggaataa
      541 caggcaatca tgctcagaca aagcttctgg cgcttgagga atccctttcc gttgcaagta
      601 agccggtgcg gcacaaaaca gcacgggtac cgtagcaatt ttcctggcca ctagacctgg
      661 ctccaattgt tgtttaaaac ggatgctaat atcgatatct tccgtcacgt gatccaggct
      721 gtggtccgat agcaccaatt caattgagac ctttgggaag ttgcgtgtaa aggttggaat
      781 cagcggcatc agcacgtgac ggccaaaggc caccgatgat cctattcgca agcggccctg
      841 tggctggcca tgaaactcgg tgatgacgga ttgcgccctc gccaatgcct ctatggtatg
      901 ttgtacttcg gcataataaa gctcaccact ctctgtcatc gccagcttgc gtgtggtacg
      961 tatcagcagc cgtgtcccca gcgacttttc cagacgctgg atattttgac tggccgcagc
     1021 agccgtgatc ccttgcaacc gcgccccagc tgcaatgcta ccggcctgaa ctgctttgac
     1081 aaaactctct atgcttctta atgtatccat cagctatcga cacagacaat atattcgata
     1141 gtttatcttt atagtgattc aatattcaag agactactcc aaggttttgt atcgttttat
     1201 agtgcctaca ctgattggag agcatgatga atcaaacaaa aaacagttcc cgcaaaatcc
     1261 caaaaaggtt cacgccttat gtgtttgctt tttttatggc agggatcatg gcttttttaa
     1321 tgtccatggt cattgttgcg gctaacagcg ggttacacgc cggttatgta ttgcgagtac
     1381 tcaatgctta tgcgcttgca atgccggtcg cattttgctg tgtcttaatc gtgaggccta
     1441 tcgtgatgca tctcgtcaca cggctggttg acggacatta gtggcgcttc aaaaataaaa
     1501 acggatgcct aggcatccgt ttttatttca acactatgct aaaacagggg tgcatacagc
     1561 ataacgatta aataaccgat agctgcccca gccagcacat cgctaggata gtgcaggccc
     1621 aacacgacgc gtgacatccc taccagtacg acaaaggggt acacaatagg aaataattgc
     1681 gggtaataat ggaccgccac catgccaaag gccactgcgt gcaatgtatg accagacggg
     1741 aaactaaaat aatccagggg ggtgccactt aatagtacat cctggcgaac ctggaatggt
     1801 ctcggacggc ttgtccgatg tttgagccat ttgtaaacca gtgtaccagt caagcctgca
     1861 gcaagcatat gcagacatac gtataaacct tgctgacctt gagtgacagc aatcatcaac
     1921 atcaacagat accagaacat gccatcgccc atgcgagaaa tggcacgaaa cagactacga
     1981 atgaagcggt attgcccggt atggctgact ttcacacaca accggctatc taccgactgc
     2041 acccatccca gtgtttgttg taatttcagc atcacaagtc ctttccgggt attcacccga
     2101 ctatgctttt tgggtaagca tagtctgact gtgatgcttg aaactatcgt gaagaattga
     2161 tgaagttaat atgacgcgcg attacttcca cagcacgcga taaataaaac caggaaatga
     2221 caagaccagg aacacacaca aggtaatgct gtaatattcc cagccttgtg gccagacctg
     2281 gcccataaca aaacgttcta ccaggtaagc aattcccagt gtaatgaagt aagcaggcag
     2341 taaggctaca aaacgctgcc acaattgggt tagtgaacct gcgatgccca accatttgtc
     2401 ggataccaaa ttcaggttag ccatgaatag catcaataat aaaaataata gtttgattgc
     2461 cataggacta ttgcaaactg attaacaggc tgtgaccaca aacactgagc agtatctcag
     2521 ggaataagcc caagcccaac aaagccaggg catgcacact gagaataaag cgcatatcta
     2581 cgggtgcagt gagcggtgtg tgatcttcag gcgcatcaaa atacattttg cgtacgatat
     2641 tgaggtaata gaaggcacca atggtagcca tgagtaccgc cagcacaacc tgccaggtaa
     2701 acccagcctg ccatgccgct tgcagcacag taaacttggc ataaaaacct atggttggcg
     2761 gaatgcctgc catactgaac atgatgacca gcatcaggaa agcaatccat ggatgacgct
     2821 gattcaagcc tttgaggtca ttgatttctt cagcttcaaa gccttggcga ctcagtaaca
     2881 ggatgatgcc aaagcctgca atcgacatca gcacataggc gctaatgtag aaaaagcttg
     2941 aagcaaagcc attggcattc gcactcatca tgccaaacat gataaagcca acatgcgaga
     3001 tcgttgagta cgccaacatg cgcttgaggt tagtttgtac aatcgcgctg aagttaccaa
     3061 tgatgataga cagcacagcc attaaaacaa gcatatcctg ccagtccaat gccaacgggt
     3121 gcaggccttg gaccaacaaa cgtactgtca tcgcataggc ggccagcttg ggcactgaac
     3181 cgatcagcaa agtcatcggc gtcggcgagc cctgatagac atctggcacc cacatctgga
     3241 acggcacagc accaaacttg aacgcaatcc cggccaccac aaacaccagg cccatgatta
     3301 acaccggact cttaggctgc tgcacaatat tattggcaat atcgctcaca tcgaggctac
     3361 cagtcatacc atacagcatg ctcatcccat ataacaacat accggatgcc agcgcaccca
     3421 atacaaagta cttcatggca gattcggatg ctttcggatt atctcggtca aacgcgacta
     3481 atgagtatag gcacagtgac aacaactcca ggccaatata aatcgtcagc aggttatggc
     3541 cggacaccat aatcatcatg cctagcaggc caaacaatac catggcataa aactcgccac
     3601 gatacagatt acggtcttgc aggtatttgc gtgtatagac gaggataagt gatgtgccca
     3661 gatacatcat catcttgatg acatcagcca acgtatcgtc gataaacatg tgcgagaagg
     3721 caaagccaac tgcaggtgta tgcgtcttaa aagtaaagaa agccgcagca aacagggtga
     3781 actggctcag gccgtatatc atgaaacgat tttgtggcag gatgaacaaa tccagcaaca
     3841 aaataaacat ggccatgccc aatactacta gttccggcaa cagggcgagc aagtcgaatt
     3901 gcatattatc cattttataa atcctgcctt aatagcctgt gacgggcaat tttgaaatcg
     3961 ccatatgttt caggaattct gtacttgtcg cctgcatgac ctctgtcaga ggttccggat
     4021 acacaccaaa accgattacc agcacggcca aaatacccag gatcaggaac tcacgtttat
     4081 tgagatcgct gagttctgcg acgtggtggt tagtcacgtt gccaaagaac acgcgcttgg
     4141 tcatccacaa ggtataggct gccccaaaaa tcaaggtcgt tgctgcgaag aaagccaccc
     4201 aaaaattgaa tttcactgcg gctaatatca ccataaactc accaacgaaa cctgaggtgc
     4261 ctggcaaacc actgttcgcc atggcaaaca ggacggcaaa agccgcaaac actggcatct
     4321 tgttgacgac accgccataa gccgaaatct cacggctatg tacgcggtca tacagcacgc
     4381 cgacactgag gaacatggca gaagaaataa agccatgtga aatcatctgc accatggctc
     4441 cctcaagcgc cagttggccg aacataaaaa agcccagtgt gacaaagccc atatgtgaga
     4501 tggaggaata agcaatcagc ttcttcatgt ctttttgcac cagggcgacc agcgcgatat
     4561 aaacgactgc aatcaaagac aaggcaatca tggcttggct gaagtaatgc gcagcatccg
     4621 gtgcaattgg catggcaaat cgtaggaaac tgtagccgcc gagcttcaag gcaattgccg
     4681 ccagcaccac ggaaccaccc gtaggcgctt ccacgtgggc atctggcaac caggtgtgga
     4741 ctggccacat cgggattttc acggcaaaag ccataaagaa agcaataaag atataaatct
     4801 gtgcctgcat gctcagtggc aacaggtagt agtcagccag ttcaaaacta cctgtctggt
     4861 ggtacaggta aataaaggca accagcatca gcaaagagcc gagcaaggta tacaggaaaa
     4921 acttgatggt tgcgtaaacg cggttaggtc caccccagat accgatcacc aggaacattg
     4981 gaatcagcat ggcttcccag aatacataat aaagaatggc atccagcgca gtgaacacgc
     5041 caatcatgat gccactcata atcaggaagg ccgccatgta ttgggcgaca tgtttggtaa
     5101 tgacttccca gccagccagc accacaatca cggttgtaaa gctggtcaat agcactagcg
     5161 gcatggagaa accatcgatg cccaagtgat aatgcatatt gaatgccggc acccatggta
     5221 ataactcttc aaactggaag aaaccatcct gtacatcaaa ctgcgtgtac aagggcaccg
     5281 tcaccaggaa actgaccagg ctgccagcca gtgccagcca gcgtgtcaat gtggccttgt
     5341 ggtcaccccc tagcaccagt accagcacac cggagagcac cggtagccag acggccagac
     5401 ttaaccagga aatacttgtg aggtcggctt gcatcattag ttaattttga taaagaaagt
     5461 catgagtagg aatacgccaa taatcatggc aaaggcgtag tgataaatta aaccggattg
     5521 caggtgacgt attttggcag cgatcttacc catcgtgtta gccgtaccat tgaccatggc
     5581 accatcaatc agtgtgatat caccaaattt ccataacagg ctacccaggc aacgcgaacc
     5641 accagcaaaa aacttctcgt taaagctgtc aaagccgtat ttgttttcca ggatattgta
     5701 gatcacgata aaccgtacct tgatggctgc cgggatatcc gggcgcttca tatagaagaa
     5761 ccatgacagc aacacccctg aagccgccag gaagaatggc aggctggtaa agccgtgaat
     5821 cgccatgccc gccgggctat gcatgaattc gtggtaatga tgcgccagat gctccattgc
     5881 cggatgcgct tcaggattga caaaaatcac attcttgaag aattcaccgt acaacatcgg
     5941 ctctagagca atgtagccaa tcaccaggga tggaatcgcc agcaaggcca atggcagagt
     6001 taccacccag cctggctcat gcgggttgtc gtttggactt agtccatggt gatgctcttc
     6061 gtcgtgtgca tggtcctcat catgatgtgc gtcgtcatgg tgggcatctg catgtgctgc
     6121 atgcgcatgg ccatcatgtt tggcatggcg ccatttttct tcaccatgga ataccaggaa
     6181 atacaagcgg aaagaataga acgcagtgac aaatacgcca acgatcacgg caaagtaagc
     6241 aaagccgcta ccaggaatat gtgacagctc aaccgcctcg ataatgctgt ccttggaata
     6301 gaacccggca aagaatggtg taccaatcaa ggccagggaa ccaatcaggg aggtaatcca
     6361 ggtcacaggc atatactttt taaggttacc catattacgg atatcctggt catggtgcat
     6421 acccataatc actgaaccgg ccccaaggaa caacagcgct ttaaagaaag catgcgtcat
     6481 caggtggaaa atcgccactg aataggcaga agcccccaat gccactgtca tatagcccaa
     6541 ctgtgacagc gtggaatacg cgaccacacg cttgatatcg ttctggataa tgccaagaaa
     6601 gcccataaac agcgctgtaa tcgcgccaat caccatcacg gttgacaaag cggtggtcga
     6661 caattcatat agtggagaca tacgcgccac cataaagata ccagctgtca ccatggtcgc
     6721 cgcgtggatc agggctgaaa tcggtgttgg gccttccatc gaatccggca accagacatg
     6781 caatggcact tgggctgatt tacccatggc accaacaaac agcaacaggc aagtgactgt
     6841 cattaacgac caattgcagc tacccagtaa atttacctgg gtgttcgcca actctggtgc
     6901 gcgctggaac acagtggcgt aatccagggt accaaagtag taaagcacca tgccgatgcc
     6961 cagcaagaaa ccaaagtccc ctacccggtt gaccaggaag gccttgaggt tagcgtaaat
     7021 cgcagttgga cgtgtaaacc agaaaccgat caacaggtat gacaccaggc ccacggcttc
     7081 ccagccaaag aacaattgca tgaagttgtt actcatgacc agcatcagca tggagaaggt
     7141 aaacaatgaa atatagctga agaagcggct gtaacctggg tcctctgcca tatagcctat
     7201 cgtgtagata tgcaccatga gtgaaacgaa ggtcaccaca accatcatca tcgcgctcag
     7261 gttatcaatc aggaacccca cttcgaaatg cacgtcgcca gaattcagcc aggtatacac
     7321 ggcttcattc atggtaaatc cactcaaagt ctgattaaaa atataagcag acaaggcaaa
     7381 agctgcgcct acaccagcga tggtcagcac atgcgcagcc catcgcggca aaaagttacg
     7441 gaaaacaccc actaatgcgg cagccattaa cggcagcaat ggaatcaata tcaggatttg
     7501 ttttattgtc atcacactta accttttaac tgatccagat catcgacatt gatggtgcgc
     7561 aagttacgga acaagaccac cagaatcgcg aggccaatcg cagactctgc cgcggccacg
     7621 gtcaggataa agaacacaaa gacctgacca gcaatatcgt tcaggtaatg tgaaaaggcg
     7681 ataaagttga gattcacagc caacagcatt aactcgatgg ccatcagcaa aatgatcacg
     7741 tttttgcggt tgaggaaaat accaatcaca ctgattgcaa acatcagcgc gcctaaaatc
     7801 aggtaatgtg acaaaccaac catgattatt ttttctcctc tgccgctgcg gctgccggtg
     7861 cttcttctat ctcggcaggc atggacacaa tgcgcaggcg gtcattgcgt ttcactttga
     7921 cctgttcggc cgggttcatg gatttgttat ctttacgttc acgcaaagtc agcacaatcg
     7981 cagcaatcat cgccactaac aacacgaccg ctgctagttc aaatggcaac aggtaatcgg
     8041 tgtataacac cttgcccaat gcagcggtat tgctgtaatt ggcaggcaat tcaacggctt
     8101 cgctaggatg caggttcttc tccgcaagca ccatggtcat ttccaccacc atcaacacgc
     8161 cgatcaaccc cgccatgggc agatattccc agaacccttc gcgcaatttg tcgaggttaa
     8221 tatccagcat catcactaca aacaggaaca acaccattac tgcgcccaca tacaccagaa
     8281 tcagcgcaat agcgaggaac tccgcctcca gcagcagcca gatacctgct gccgtaaaaa
     8341 acgccaacac cagaaacaac gccgcatgca caggattacg cgtggtaatc acacgtatgc
     8401 cggaaaacag cagaatcgct gccagcacgt aaaaaacaat atcggtaaat aacatgattc
     8461 ctgcccgccc ctatcgatag accttgtcct gggcgcggtc agctgcaatc tgcgcctcgt
     8521 acttatcacc tacttctagc agcatctgct tgttataaag caaatcacca cgttgttcac
     8581 cgtgataatc aaaaatacgg gtttctacaa tactgtccac cggacaggac tcctcgcaaa
     8641 aaccacaaaa aatgcacttg gtcaggtcaa tgtcataccg cgtggtccgg cgtgtaccat
     8701 cttcgcgctg gtcagactca atggtaatcg ccatcgccgg gcacaccgct tcacagagtt
     8761 tgcaagcaat acagcgctct tcgccattcg ggtaacggcg caacgcgtgc aagccacgga
     8821 aacggttgga ttgtggagtc cgttcttccg ggtattgaat cgtgattttc ttggcaaaga
     8881 aatagcgccc agtcagagcc atgcctttca gcatttccca taacatcaaa ctgcctaatg
     8941 tacttttaat cgaattgaac atggtaaggt ttccaaaatc cctgtctgtg cgttaaccac
     9001 ttagtggaac aagtaagccc actgggtttg catcatcgcc ccaaccacaa taatccagac
     9061 cagtgtgatg ggaataaaga ctttccagcc cagacgcatg atctggtcat agcggtaacg
     9121 cggaaaggtc gcgcggaacc acagaaacag gaacaataag aatgcaacct ttgccagcaa
     9181 ccacaggatg ctatctggca ggaatggcac tggggacaac cagccaccca ggaacatcaa
     9241 tgccgccagc atggagacca gcatcatgtt ggcatattcg gctaagaaga acaccgcaaa
     9301 tgccatacca gaatactcaa cgtggaaacc agccacgatc tcggactcac cttcggcgac
     9361 gtcaaacggc gcacgattgg tttcagccac ggcactaatg aaatagacca caaaaagtgg
     9421 gaacaatggc aggaaatacc agtgccagaa accgccctcc tgccccatga caatcttgcc
     9481 caggtttagc gaattcgcgc acataagcac gccaacgagg gtaaagccca tggcaatttc
     9541 ataagaaacg atttgtgccg cagagcgcaa agacccgagg aacgcgtatt tggagttgga
     9601 tgcccaaccg gcaataatca cgccatatac cgccacagag gtcatggcca ggatatagag
     9661 taaacctgca tcaatattgg ctaataccag cgtcgcatca aatggaacaa ccgcccaggc
     9721 agcaaaggct ggtgctatcg ctaacaccgg accgataaag aacaaagcct tgttagaagc
     9781 ggttggcagg atgatttctt taaacagaag cttcaaacca tcagccaaag gttgtaacag
     9841 tccaaaataa ccaacacggt ttggaccaat acgcacctgc atatagccaa tgaccttacg
     9901 ctcagccagc gtcagatatg caaccccaat catcaatggc aacacaatgg ccacgatttt
     9961 aagcaacgtc cacactacca gttgtacgtc tgcccagtag gaaccaaata attcagcaaa
    10021 gaattgcatg ttatgccatc tcggtcagtt gtttttcgta aatcgccact tgttcggccg
    10081 aggccttttc aagggtgata tcccccatga ggtcgcccaa gcctgccgtg agtggatgcg
    10141 ccgcagccac ccggacacag cctttggcca catgattatc cagacgcacc ttgagtacgg
    10201 cactgccttt atcctgacgt gcaaccacga tatcgccatc cgccaggcct agttcagcca
    10261 attgctcggc acgcatacgt gccatggggc gaatgttgta tttggttttt tgcaatggcg
    10321 ctgagcgacg tacaatcggg tcaaattcgt attgcggcac ttcaccaata cgttgcaagc
    10381 cttctttctt gatattgacc tgaatttcca ccaactcttt caagttgttg ttcaagctgc
    10441 gccacacgac ggctgaaggt ttttcgccat tgaaaatggc atttttaacc tcttcggagc
    10501 tgttgtactc gaaaccggac aagcccaatg tattggccag tacgcgcagc accttccagc
    10561 ctggccgtgc ttcgcctaat ggccgagtca cggcctggaa gctttgcaca cggccttcca
    10621 tactcataaa ggtgcctgat gtttcagtga aaggtgtgat cggcaacagg atatctgcat
    10681 tctcaagcgc tgaagatttg taggcagtca gtgcaatcac gcactcggct ttagccatgg
    10741 ccgcactggc caaggcagca tgctggcagt ccagctccgg ttcaatattc aataagaggt
    10801 aggccttgcg tggcacctcc agcatggcgc gtgcatgcat accggttgca gatggcatga
    10861 cgcccatcag gtgcatgcct gtactgtttg ccgctggtgg caaaatgcca cctttggcac
    10921 cagtaatgcc gccaatcgcc tcagccatac tatacatttc ggtaaaacgt gggtcgctca
    10981 aggccatatt gccgaggaaa atcccaacct tgggtgcaat caggatcaac tcttcactga
    11041 taccagccag acactcagca atcttttgcg tgtttggtct aacctgaatt tcttccagca
    11101 gcttgttcaa tgccggaggc aagcataaag acaatttttg caaaccggac atggctttga
    11161 ggatttgacc gagtacattg accatatcat ttggacgcac aatcaccttg tgcgcaatat
    11221 ccatcaatgg gtcattatcc agcgggctga caatacataa ctcagcacca ttcgccaccg
    11281 ccttgcggat gcgctgcgcc aacaacggat gctcattacg caggttagaa ccaatcagca
    11341 ggatacgatc tagctcttcg atttcctgga tattacagcc catccatggt gtgcccgtgc
    11401 gtttgccatc caggcggaaa tcggtctgac gcagacggta atccacatta ccgcagccaa
    11461 ggccctcagc caattgtttg atcaggtagc cttcttccat ggtgctgttt ggggaaacca
    11521 gcacgcctag cgcatcgccg ccatattcct tggtgatgtc ttttaattgg cctgcggcaa
    11581 attccagtgc ggttttccag tcagtctcct gccactgccc attgtgtttg atcattggca
    11641 cttttaaacg gtcaggactg ttcaggcctt cataagaaaa acggtcacgg tcagagagcc
    11701 agcactcgtt aatcgactct ttttcacgcg gcaatacgcg catgacctta ttgtctttaa
    11761 cgtgtacctc aatatgcgaa cccagaccat catgcggggc aatcgacgga cgacgcacca
    11821 gctcccagct gcgggcagaa tagcggaaag gcttactggt caatgccccc accgggcaca
    11881 aatcgataat gttaccactg agctctgaat tcacagactg gtcaacataa gcggtaattt
    11941 cagcatgttc gccacggttt accagaccta attcttgcat gccaccgacc tctttgagga
    12001 agcgcacaca gcgtgtacat tgaatacaac gtgtcatatc ggtgctcact agcggaccga
    12061 tatttttatt gaacaccaca cgtttttctt cggtatagcg agagccagag gcaccgtaag
    12121 ccacggcgat atcctgcaaa tcacattcgc cgccctgatc acaaatcgga caatccaaag
    12181 gatggttaat cagcaaaaat tccatgacgc tttgctgtgc ttcaacggct gacttgctac
    12241 gggtataaat cttcatgcca tccgctacag gtgtcgcaca ggctggcaat ggtttattaa
    12301 acttctccac ctgcaccagg cacatacgac agttggccgc taccgacagc tttttgtgat
    12361 aacaaaaacg tggaatcgcg atccctgccg catctgcagc atcaataatg gtgctgccgt
    12421 gttcgacctc tagtggtttg ccgtcaattt caatgtttag catgtgatta ccgttaagct
    12481 tgatagcgtt tatttaccat cgaccatgga cttgccatgc tggatgtaat attcaaattc
    12541 tggacggaag tgtttgatga agcttaacac cggcgtcgca gcagcatctc ccagcgcgca
    12601 aatggtgcgg ccagaaatgt tattgctcaa atctgtcagc aggtccagat cttccatttt
    12661 gcccttgcca tcgacaatac gttcaatgat ccggtagacc cagcctgtac cttcacgaca
    12721 gggcgtgcac tgaccacagc tttcttcata aaagaagtaa ctcaggcgtt taagtgtttt
    12781 caccatacag gtcgtttcgt ccatcacaat catggacccc gcgcccaggc tggagcctgc
    12841 tttgctcagg ccatcgtagt ccatggtcgc gttttcaatg gcgctggctg gcatcactgc
    12901 cgtggaagga ccgcctggaa tcacggcctt tagctgacgg cctttccaga taccaccggc
    12961 catctccagc aactctttaa atggtgtacc catcttgact tcatagttgc ctggtttttc
    13021 tacatgacca gacaccgaaa acaatttact gccaccagag ttaggcaccc caaggtcagc
    13081 aaatgcctga ccgccatgct gcataatcca ggggatagac gcatagcttt ccgtattatt
    13141 tacattggtt ggcttgccaa acacaccata actcgcaggg aaaggcggtt tgaaacgtgg
    13201 ttgccctttt ttgccttcaa tagactcaat caacgcagtt tcttcaccac agatatacgc
    13261 gccatagcca tgcacggcgt agaggtcgaa actgaattca gtaccaaaca ggttttcacc
    13321 cagataacct gcttcgcgcg cttcagccaa ggcagcttca aaaatctcgt aatcctgcca
    13381 gatttctcca tggatatagt tgtagcctac acgtgcgccc aaggtatacg cggcaatcgc
    13441 catcccttcg atcaactgat gcgggttgta acggataatg tcgcgatctt taaatgtgcc
    13501 cggctcacct tcgtccgtat tacagaccag gtacttggta ccgtcgtaat accgtggcat
    13561 aaaactccac ttgaggccag tcggaaaacc tgcgccacca cggccacgca aattagatac
    13621 tttgacctga ccaataatat caggcgcttt gatcttctca gtcaccagac gcttgagttg
    13681 cgcatagccc cctaattttt cataagttgc taacgaagcg gggtttgctt ccgtgagtgt
    13741 tcgtaaggtc accagtgttt ggccagccaa atcggtcttg accactggct tactacttac
    13801 aatcgcattt gaagtctttg ccatgttcag ttccttagcc cttgctcagc tcgttaatca
    13861 gggcatccac tttttcagtg gtcagaaatt cgtgcatttc actattgttc acatgcaaca
    13921 aaggtgcgcc accacaacag cccatgcact cgccttcttt caggcaaaac ttgccgtccg
    13981 gggtcacttc gttgaacccc acacccagct tgtgctggag gtgttccaca atttcatcag
    14041 aaccacgcaa catacaagag atattggtac agatcgtaat cttgtgctgc cctactggct
    14101 cgagatcgta catattgtag aaagtcgcca cttccagtgc agcgatttcg gggatacgca
    14161 aatacttggc aacctcggaa atactttcct tggataacca gccacgttcc atttgcacaa
    14221 tccgcaaagc ggacatcacg gctgcctgac gtctgtccgc tggatatttg gacagttcgt
    14281 attcaatttt ttcaactgat tctgcactga gcatgcttga cccttactat ctttccgatc
    14341 cgtgctatct gttcattctg tgtaagcgtt ctgcttatct gtcgatttcg ccgaacacaa
    14401 tatcctgtgt accaataatt gcgaccaggt cagcaatcat gtggccacgt gtcatttcat
    14461 ccagcgcagc cagatgggca aacccaggcg cacggatctt caggcgatat ggcttgtttg
    14521 caccgtctga caccatataa ataccaaact cgccttttgg gtgctcgact gctgcatagg
    14581 cttcgcctgc tggcacatgg aaaccctcgg taaacaactt gaagtggtgg atcaagtctt
    14641 ccatattggt tttcatgttc tcgcggttag gcggtgcaat cttattatca tcagagatga
    14701 ctgcacccgg attcttgcgc aaccagtcta tgcactgttt gacgatacga ttagattggc
    14761 gcatctcttc cacgcgcacc aggtaacggt cataacagtc tccgtttaca ccaatcggga
    14821 tatcaaaatc catcttgtca taaacttcat atggctgctt cttgcgcaaa tcccaggcaa
    14881 tgcctgagcc gcgcaacata gcaccggtca atccgagcgc cagcgctcgc tccggcgtaa
    14941 ccacaccaat acccaccgta cgctgcttcc agatacggtt atctgtcagc aaagtttcat
    15001 attcatcaac ataagtcgga aagcggttgg taaagtcttc aatgaaatcc agcatagaac
    15061 cctggcggtt ttcattgagc tttttgatct cgtctgcact gcggaacttg gtcgtttcgt
    15121 gctgcggcat tttttccggc aagtcacgat acacgccgcc cggacggtag taagccgcat
    15181 gcatacgtgc accagatacg gcctcgtaga catcgaatag gtcctcgcgc tcacggaagg
    15241 cgtacaaaaa taccgtcatc gcacccacgt cgagcgcatg tgcgcccaac cacaacaagt
    15301 ggttgaggat acgggtaatt tcgtcgaaca tgacacggat atattgcgca cgcacaggca
    15361 catcaatgcc cagcattttt tcaatggcca tgacatacgc atgctcgtta gccatcatgg
    15421 acacataatc cagacggtcc atataaggca cgctttgcaa ataggtgcgg ttttcggcca
    15481 gtttttcggt ggctctgtgc agcaacccaa tatgcggatc ggcacgctga atgacttcgc
    15541 catccagctc cagcaccagt ctcaacaccc catgcgccgc agggtgctgc gggccgaagt
    15601 tcatcgtata gttcttaatt tcagccatga tgcgcccctt cgtcacggat gacacgcggg
    15661 acgttgtttc tgagttcaac tgaaacaggt tgataaatca cgcgctgctg cacaggatca
    15721 tagcgcattt caacgttacc aatcataggg aagtctttgc ggaatggatg accgacaaag
    15781 ccgtaatctg tcagcaagcg ccgcaaatca ggatggttct caaacatgat gccgtaaagg
    15841 tcaaatgctt cacgctcgaa ccagttagcc actggccaaa tatcaac
//
LOCUS       DATMSK010000048         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_309595,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000048 DATMSK010000000
VERSION     DATMSK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309595"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VNZ84_01655"
     CDS             complement(<1..187)
                     /locus_tag="VNZ84_01655"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882798.1"
                     /note="catalyzes the formation of pyruvate and glyoxylate
                     from 4-hydroxy-2-oxoglutarate; or pyruvate and
                     D-glyceraldehyde 3-phosphate from
                     2-dehydro-3-deoxy-D-glyconate 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="keto-deoxy-phosphogluconate aldolase"
                     /protein_id="HWU65684.1"
                     /translation="MSTLELANHGPVIPVIVINKVEDAVPMAEALLEGGVKVLEVTLR
                     SPVALQAMEQIAKHVPDA"
     gene            complement(331..2145)
                     /gene="edd"
                     /locus_tag="VNZ84_01660"
     CDS             complement(331..2145)
                     /gene="edd"
                     /locus_tag="VNZ84_01660"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HWU65685.1"
                     /translation="MKQSHIEVTERIIERSRPTRKAYLQRIDDYLNREKGPDRLGCAN
                     VAHAFAAMPQNDKFKIYAEKPTHIGIVTAYNEMLSAHQPYVNYPDIIRDEARKHGATV
                     QVAGGVPAMCDGITQGEPGMELSLFSRDTIAMSTAVALSHDVFDASLLLGVCDKIVPG
                     LLIGALHFGHLPCVFVPAGPMSTGIDNTSKSKVREKYAQGLCDRDELLASESAAYHGA
                     GTCTFYGTANSNQMLLEAMGLHVPGAAFIHPHDGMREDLTREAVRMVIQNTQKNKFVP
                     IGRLVDEHVIVNAMVALLATGGSTNHLIHWVAVARAAGIIIDWTDFHYLAKATPLLAS
                     VYPNGKADVNEFQTAGGPGFVIRELIEGGYMYPDVYTVSGGGMHDYGKKPEKEGDKLV
                     WKDYPAESGDETIVRTAAHPFNESGGLRLLKGNLGRSVIKISAVPEDRHIIEAPAIVF
                     DAQEELLAAFDRGELERDFIAVVRFQGPKANGMPELHKLTPPLAVLQNKGFRVAIVTD
                     GRMSGASGKIPAAIHLSPEASAGGAIAKIRNGDIIRLNGTVGQLNVLVDEDTWAEREA
                     EELSDAKRHYNSHGLGRELFGGMRRNVLSAEEGAVTWL"
     gene            complement(2404..>3603)
                     /locus_tag="VNZ84_01665"
     CDS             complement(2404..>3603)
                     /locus_tag="VNZ84_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HWU65686.1"
                     /translation="IKEFYDPDSLGGLYGAITEFLGFEMLDGEFKVMGMAPYGDPNKY
                     DLSRLAKFENGELIINTEYVNVVGLRRYKENGKGYYFTPKLIEWLGPKREGDEIDNPY
                     IHYAASVQQLLEDLALQMIDYYLGDIIRETGKIVMAGGVALNVKLNQRIISLPHVKEL
                     FVQPASGDNGTSLGAATYAAHLAGETIHKMEHAYLGPAYTNEECIAACEVHPAKPIFT
                     RVEDGPRKAAELLAAGNPLAWFQGRMEFGPRALGCRSILGDPSFPGVADRINAQIKYR
                     ERWRPFCPSMLDRVAEDILQTDHPAPYMTFTFDVNPAWKARIPEVVHEDGTARAQVVT
                     KATNPRYYALIEHLEQLRGNGVVLNTSLNRRGEPMICSPTDALNMFFGCDLEYLMLED
                     ILVTKSA"
BASE COUNT          900 a          981 c          831 g          891 t
ORIGIN      
        1 tcgcatcagg cacatgtttg gcaatttgtt ccatggcttg cagggcgact ggagaacgca
       61 atgtcacttc cagtacttta acgccacctt ctaacaaagc ctcagccatt ggcacggcat
      121 cttctacttt gttaataaca atcaccggaa tcaccgggcc atggtttgct aactctaatg
      181 tgctcatgtt atgaatattg acctaaataa ataaaaaaaa tttaaccgct tgtttaatag
      241 tgaattaaaa gtgctaagca agactaagcg caggcagtac gattagtacg gcaagcgaac
      301 tgtaacgacg tattgcgacg caattttaat ttacaaccaa gtgacagcgc cttcttcggc
      361 agacaacaca tttctacgca tgccgccaaa caactcacgc cccaaaccat gagagttgta
      421 atggcgcttg gcatcgctga gctcttcggc ttcgcgttct gcccaggtat cttcgtctac
      481 cagtacgttc aattggccta cagtaccatt gaggcggatg atgtcgccat tgcggatttt
      541 ggcaatggcg ccaccagcac tggcttcagg gcttaggtga atcgccgctg gaatcttgcc
      601 agacgcgcca ctcatgcggc catcagtcac aatcgctaca cggaagccct tgttttgtag
      661 cacagccagt ggcggtgtca gtttatgcaa ctcaggcata ccattggctt taggaccctg
      721 gaaacgcacc acggcaataa agtctctttc cagctcgcca cggtcaaacg cagccagcaa
      781 ttcctcttgt gcatcaaaca caatcgccgg tgcttcaatg atgtgacggt cttcaggcac
      841 ggcggaaatc ttgatcacgc tacgcccgag gttacctttg agcaatcgta agccgccaga
      901 ctcattaaat ggatgtgctg ccgtgcgcac gatcgtttca tcgccacttt cagccggata
      961 atctttccag accagcttgt cgccttcttt ttctggtttt ttaccgtagt catgcatgcc
     1021 gccgccactg accgtgtata catccgggta catgtagcca ccctcgatca gctcacggat
     1081 cacgaaacca ggaccaccag cggtctggaa ttcgttgaca tcggctttgc cattcggata
     1141 gacgcttgcc agcaatggtg tagccttcgc caggtaatgg aagtctgtcc agtcaatgat
     1201 aatgcccgct gcacgcgcaa cggccaccca gtggatcaaa tggttggtcg agccgccggt
     1261 ggccagcaac gccaccatcg cattcacaat cacgtgctca tctaccaggc gaccgatagg
     1321 cacaaatttg tttttctggg tattttgtat caccatgcgc accgcttcgc gggtcaggtc
     1381 ttcgcgcatg ccatcatgtg gatggataaa ggccgcaccc ggcacatgca ggcccatggc
     1441 ttccagcaac atctggttac tgttggccgt gccgtagaat gtacaggtac cagcaccgtg
     1501 gtaagccgct gactcactgg ctaaaagttc atcacggtca cacagacctt gtgcgtattt
     1561 ttcacgcacc ttggatttcg aagtgttatc aatgcctgtg ctcattggtc cagcaggcac
     1621 aaacacacat ggcaaatgac caaaatgcaa ggcgccgatc aacaagcctg gaacgatttt
     1681 gtcacagacc cccaacagca aggatgcatc aaagacatca tgtgacaaag ccaccgcagt
     1741 actcatggca atggtatcgc gactgaacag gctcagttcc atacctggct cgccctgcgt
     1801 aataccgtca cacatcgctg gcacaccgcc agcaacttgt accgtcgccc catgtttacg
     1861 cgcttcatca cggatgatgt ctgggtagtt cacatacggc tgatgggccg acaacatttc
     1921 gttgtaagcg gtcacaatcc caatatgggt tggtttttcg gcgtaaatct tgaacttgtc
     1981 gttctgcggc atcgccgcaa atgcgtgtgc cacgttggca cagcccagac gatccgggcc
     2041 tttttcgcgg ttcaggtaat catcaatacg ctgcaaatac gccttacggg tcggacggct
     2101 gcgctcgatg atgcgttctg ttacttcaat gtgagattgt ttcattgact atcgctcaac
     2161 tgtgttttaa ctacctgatg aactatccta gatgaactgc tttgaacttg ctatttagtc
     2221 acagtttagg gtcgtaatat atatgaatcc aaacaggttc cctacctgcg attatcgcca
     2281 aactggtgca cgacgtcaaa ctgaattacc aaaattgatg gtttttttag aggattcaat
     2341 gacttaaaac aatatacact gtttagacaa agcggcttga agcgacaagc ctcaagccag
     2401 ccttcaggcg gatttagtga ctaagatatc ttccagcatc agatattcaa gatcacagcc
     2461 aaagaacata ttcaaggcat cggtcgggct gcaaatcata ggttcaccgc ggcggttcag
     2521 tgaagtgtta agtacgacac cattaccacg caattgctcc aaatgttcaa tcagtgcgta
     2581 ataacgtgga ttcgtcgcct tggtcaccac ttgcgcacgt gctgtgccgt cttcatgcac
     2641 cacttcaggg atacgcgctt tccaagccgg atttacatca aaggtaaagg tcatatacgg
     2701 cgccggatga tctgtttgca aaatatcttc tgccacacgg tccagcatac tgggacagaa
     2761 tggccgccag cgttcacggt atttgatctg cgcgttgatg cgatcagcca caccggggaa
     2821 gctaggatcc cccaagatac tgcggcaacc caaagcacgc ggaccaaact ccatacggcc
     2881 ctgaaaccac gccagtggat tacctgccgc taacaattca gctgctttac gcgggccatc
     2941 ttcaactctg gtaaaaattg gtttggccgg atgcacctca caggcggcga tacattcttc
     3001 attggtatac gctggcccca ggtaggcatg ttccatttta tgaatagttt cacctgccaa
     3061 gtgagcggca tacgtagccg cgcccaggct ggtgccgtta tcgccagaag ccggctgcac
     3121 aaataactct ttcacatgcg gcagggagat aatacgctgg ttcaacttca cgttcagtgc
     3181 cacgccaccc gccatcacaa tcttgccggt ctcgcggata atatcgccca ggtagtagtc
     3241 gatcatctgc aaagccaggt cttccagcaa ctgttgcaca ctggcggcgt aatgaatgta
     3301 agggttgtcg atttcatcac cttcacgctt agggcccagc cactcaatca gtttaggtgt
     3361 gaagtagtaa cctttgccat tttccttgta gcggcgcaaa ccgaccacat tgacgtattc
     3421 ggtgttgatg ataagttcgc cattttcaaa tttggccagg cgacttaaat catatttatt
     3481 cgggtcacca taaggcgcca tgcccatgac tttaaactcg ccatccagca tttcaaagcc
     3541 caaaaattca gtgatggcgc catacaagcc acccaaggaa tccgggtcat agaattcctt
     3601 gat
//
LOCUS       DATMSK010000049         6113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_121693,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000049 DATMSK010000000
VERSION     DATMSK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121693"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VNZ84_01670"
     CDS             <1..1456
                     /locus_tag="VNZ84_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWU65687.1"
                     /translation="SLINDVLDLSKIEAGRMELEAEVTDVRLLLQNCLSIIKEKAAAQ
                     SIKLQTEFSDALGNMWLDARKVKQIVYNLLSNAVKFTPEGGQVILRAQLVERKDILKQ
                     TRGMPGSDASSIGHASQFIEIAITDTGIGIEAQDIHRLFKPFVQVESTLSRRFEGTGL
                     GLALVRKLAQLHHGAVAVQSTPGKGSTFTVWLEYNPALADPALGAPEAAERQLINAHS
                     PHVLVLEDDDRAADLIRLQLESDNCRVTRAATAEIALAILGDGVPPDLITLDILLPGM
                     DGWEFLAKLKANDVLAHIPVVIISIVADSNRGLSLGAAHVLQKPVSANDLQTALSKLG
                     FAKPENQQAGLNVLVVDDDPKAVEIISAYLEHANHSVLRAYGGTEAIHIAKLQTPDLI
                     VLDLMMPEVSGFEVVEALKLDKRTAKIPIIIVTSKFLTPDEQLTLKGQVLAVLEKSEF
                     NHGNFLSEVHRALAGKRGNHRAKGKGKRGKNTHH"
     gene            1467..1793
                     /locus_tag="VNZ84_01675"
     CDS             1467..1793
                     /locus_tag="VNZ84_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWU65688.1"
                     /translation="MNMRLAQLLLETAGHEVLQAVNALDGLEIARGQSPEMILMDIQL
                     PGMDGLTATQLLKGDANTNQIKIIALTAFAMKGDEEKMRAAGCDGYIAKPIRYKEFLE
                     TIESLL"
     gene            1842..2915
                     /locus_tag="VNZ84_01680"
     CDS             1842..2915
                     /locus_tag="VNZ84_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWU65689.1"
                     /translation="MKVTRKTILIVDDEENNRSYLNALLTSEGYATLQAHDGKMAYDM
                     ILAHRPDLILLDAMMPVLDGFELAEQLKNNEVTKVIPIIMLTALMDRASKMRALQCGV
                     EEFVTKPVDRAELWIRVRNLLRLKEYNDFLTDYSHTLEEQVEERTAQLVASYFDSMFS
                     IMRAAEFRDEETGAHIHRIAFFCQEISTDMGMPADFIDTIYHASPLHDVGKIGIPDHI
                     LLKPGPHTAEEWAIMKTHSALGDSILGHGRGTSPFMMMGAEIALNHHERWDGSGYPHG
                     LKGEEIPLSARIMMMSDVYDALRSKRPYKPAFTHEQALNIILEGDGRTQPIHFDPMVL
                     NAFKKLAHRFRDIFEAHADQSDT"
     gene            complement(3134..4318)
                     /locus_tag="VNZ84_01685"
     CDS             complement(3134..4318)
                     /locus_tag="VNZ84_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HWU65690.1"
                     /translation="MRECTLLILDDDVNAAKIIASEAQSLGFEVRIFDDAAQFIEASL
                     TEKPSHLAIDIIMPGVQGMSLLQTLAQNACQSSIMLTSGIGSDILPAAQLAAIEQNLN
                     IRGILQHPFHQHILRNMLSDQVSIDLHAHTSNDASHQSFIVDANTIDNAIRNHEFEVF
                     YQPQIDLVTGKVMGFEGLLRWRHPGLGIKLPELFIPIAEKTGQIEQLTQIVVETGFHF
                     IRSLGKSLSFSLNISPKTFKAQSLSNVLLSACQASDLPPERVVLELTEIATMGDPSQA
                     QQTLSHLRAEGFKLSLDDFGNNNTSMSQLANLPFTTLKIDKSFVTTMEVSSQSRKVVA
                     STLKLAQSLKLETIAEGIENTLAAIGLRELGCRYGQGYYFARPMDQVSTVNWLQGWNR
                     QF"
     gene            complement(4589..>6113)
                     /locus_tag="VNZ84_01690"
     CDS             complement(4589..>6113)
                     /locus_tag="VNZ84_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU65691.1"
                     /translation="FAFILRKIGTAAYIYNLLMISSAIACVMIGQIEHAYVVALLVMV
                     FEVGMLLAMHASSTVFDQWVNATYETQALLKDNQRLANQDVLTQLPNRRQFFVQVEQE
                     LLLARQTSQCFAVGILDLDNFKPVNDVHGHHIGDLVLVEVGKRLAQVRPNEVRFYRLG
                     GDEFAFHLVTDDEYGSLKQIAYEINQAISQPISIDGLLIVIKASMGACIVCDEFTSAQ
                     DLYEHADFALYHVKRTERGNLEIYSEVLEKERMHLNRIDQALRSADIQTELFPVFQPI
                     IDIDAGEVGAFESLARWNSPSIGMVSPAQFIPVAESLGMIAEITKLMFNRSISEMLTW
                     PATMRLSFNLSAYDVTNQAVIKDLISIMHSSQLQPGRFVFEITETALLQDFETARENI
                     GLLRQAGAKVALDDFGTGYSSLSHVQHLPLDKLKIDRSFIKDIETNTTSQTIVRSILA
                     LCQGMHMECVAEGAETESQVRFLQAMGCQWVQGYYFAKPMPKEQVLPYLSRANIPTH"
BASE COUNT         1548 a         1448 c         1595 g         1522 t
ORIGIN      
        1 ttcgctgatt aatgacgtgc tagacctgtc aaaaattgag gcgggacgga tggagcttga
       61 agcggaagtg acggatgtac gtttgctgct gcaaaactgc ctgtcgatta tcaaagaaaa
      121 ggcggccgcg cagagcatta aactgcaaac ggagttctct gatgccttgg gcaatatgtg
      181 gctggatgcc cgtaaggtaa aacagattgt gtataacctg ttgtccaacg ccgttaaatt
      241 cacccctgaa ggcgggcagg tcattttgcg cgctcaactg gtggagcgta aggatattct
      301 taaacaaacc aggggtatgc cgggcagcga cgcttcctct atcgggcatg ccagccagtt
      361 tattgagatt gcaattacgg acaccgggat aggcattgaa gcgcaagata ttcaccgact
      421 gtttaaaccg tttgtgcaag tggaaagcac gctttcaaga cggtttgagg gaaccggtct
      481 tggcctggct ttggtcagaa agctggctca gcttcaccat ggcgcggttg ccgtccaaag
      541 taccccaggc aagggcagca cctttacggt ctggcttgag tacaaccctg cgttagccga
      601 tcctgcgctg ggcgcaccag aagcagctga gcggcaatta atcaatgctc attcgccgca
      661 tgtgctggtg ttggaggatg atgaccgtgc tgcagacctg atccggctcc agcttgagtc
      721 ggacaattgc cgcgtcacgc gtgcagccac cgctgaaata gcgctggcga tattggggga
      781 tggtgtcccg ccagatttaa ttacgctgga tatcctgttg cctggcatgg atggctggga
      841 gtttctggcg aagctgaagg cgaatgatgt actcgcacat attcccgtgg tgattatttc
      901 gattgttgca gacagcaatc gcggcttgtc gctgggtgct gcccatgtat tgcaaaagcc
      961 ggtgtctgcc aacgacttgc aaaccgcttt atccaaactt ggctttgcca agccagaaaa
     1021 tcagcaggct ggtttaaacg tactggtggt agatgatgac cccaaggcgg tggaaattat
     1081 ttctgcttac ctggagcatg ccaatcattc ggtattgcgg gcttatggtg gaactgaagc
     1141 catccatatt gcaaaattgc aaacgcctga cctgattgtg ctggatttaa tgatgccaga
     1201 agtgagtggt tttgaagttg tcgaagcgtt gaaactggac aagcggacgg caaaaattcc
     1261 tatcatcatc gtcacctcta aattcctgac ccctgacgag caactcaccc tcaaagggca
     1321 agtgctggca gtccttgaga agtctgaatt taatcacggt aatttcttga gtgaagtgca
     1381 ccgtgcattg gcaggtaaac ggggtaacca tcgggcaaag ggtaaaggaa agcgtggcaa
     1441 aaatactcat cattgaagat aacctcatga atatgaggct ggcgcagcta ttgttggaga
     1501 cagccgggca tgaggtatta caggctgtga atgcccttga tgggctggaa attgcgcgtg
     1561 gacagtcacc cgaaatgata ttaatggata tccagttgcc aggcatggat ggcctgaccg
     1621 cgacacagtt gctgaagggg gatgccaaca ccaaccagat caaaatcatt gcgctcacag
     1681 cgttcgccat gaagggggat gaggagaaaa tgcgggcggc agggtgcgat ggctatattg
     1741 ccaagcctat ccgttataaa gagtttttgg aaaccattga gtccctgctt taaccaggct
     1801 actcaaatca atgcgctgga aagggggtaa ataaaattga catgaaagtc actcgcaaga
     1861 cgattcttat cgttgacgat gaagaaaaca atcgcagcta tctcaatgcc ctactcacct
     1921 cggaaggtta tgcgactttg caggcccacg atggaaaaat ggcctatgac atgatcctgg
     1981 cacatcggcc tgacctgatc ctgctggatg ccatgatgcc agtgctggat ggcttcgagt
     2041 tggctgagca gctcaagaac aatgaagtca caaaggtgat ccccattatt atgctcaccg
     2101 ccttaatgga cagggcatcc aaaatgcgag ccttgcaatg tggcgtagaa gagttcgtca
     2161 ccaagcctgt ggatagggcc gagctttgga tcagggtccg taatctgctg cgcctcaaag
     2221 aatataacga tttcctgacc gattacagtc atacgctgga ggaacaagtt gaagagcgga
     2281 ctgcacaatt ggttgccagc tactttgact ccatgttcag cattatgcgg gctgcagaat
     2341 tccgtgatga agaaacaggc gcgcatattc accgcattgc gtttttctgt caggagattt
     2401 ctacagatat gggcatgccg gcagacttta ttgatacgat ttaccatgcc agtcctttgc
     2461 atgatgtcgg caagattggg attccagacc atatcctgct caagcctggc ccgcatacgg
     2521 cagaagagtg ggcgatcatg aaaacccatt cagcattagg ggacagtatt ctcggtcatg
     2581 gtagaggcac gtcgccattt atgatgatgg gggccgaaat tgccctcaat catcatgagc
     2641 gctgggatgg aagcggctac cctcatggct tgaaaggtga agagatccca ctttcggcac
     2701 gtatcatgat gatgagcgac gtctatgatg cattgcgtag caagcgcccc tataaacccg
     2761 cattcacaca tgagcaggcc ttgaatatca tcctcgaagg cgatgggcgc acccaaccta
     2821 tccattttga tccgatggta ttaaacgcat tcaagaaact ggcacatcgc ttccgtgata
     2881 tttttgaagc gcatgctgat cagtctgata catgagtcga atgtgactgg aaagtgttcg
     2941 caaaggggcg gtttgaatat tcgaccagtc ggcatcacca gcgccatggg aaggccgctt
     3001 tctacgcttt cccatccgct taaactatcc gccggattta aatatgacgc aggagcctgt
     3061 gccagaatgg gtatctcgct accaactttt ctgacataca gaagtgatgg tcagtgagta
     3121 gccatactca acactagaac tgccggttcc agccctgcaa ccaattcact gtcgagacct
     3181 gatccatggg tctggcaaag taatagcctt ggccataacg gcaaccaagt tcgcgcaagc
     3241 cgatggccgc aagcgtgttt tcaatgcctt cggcaatggt ctccagtttg aggctttgcg
     3301 ccagtttcag tgtagaggca accacttttc ttgattggga ggagacctcc atggtggtga
     3361 caaacgattt atcaattttt aaggtggtaa agggcaggtt cgccaactgc gacatcgagg
     3421 tattattgtt tccaaaatca tcaagactta atttgaagcc ttctgccctg agatgtgaaa
     3481 gtgtttgttg tgcctgactg ggatccccca tggtggcgat ctcggtgagc tctaatacaa
     3541 ccctttccgg tggcaaatca ctggcttgac acgcgcttag caggacgttg ctaagactct
     3601 gcgctttaaa ggttttgggt gaaatgttca aggaaaacga gagcgacttc ccaagtgagc
     3661 gaataaagtg aaagcctgtc tcaacgacaa tttgcgttaa ttgctcaatc tgcccggttt
     3721 tctctgcaat cggaataaac agttcgggca gtttgattcc cagccccgga tgccgccagc
     3781 gcaataagcc ctcaaatccc atcaccttgc ctgtgaccag gtctatctgt ggttgataaa
     3841 atacttcgaa ctcatgattg cggatagcgt tatctatggt gttggcatcc acaataaaag
     3901 actggtggct agcatcattg ctggtatgag catgcaaatc tatggatacc tgatccgata
     3961 gcatgtttct gagaatgtgc tggtgaaaag ggtgttgcaa tataccgcgt atattaagat
     4021 tctgttcgat ggcagcgagt tgcgctgccg gcaagatgtc tgagcctatg ccactggtta
     4081 acataatgga ggactggcac gcattttggg ccagggtttg gagcagactc atgccttgaa
     4141 cgccgggcat gatgatatct atggctaaat gagagggttt ttcagttagg gacgcttcga
     4201 tgaactgcgc ggcatcatca aatatgcgga cttcaaatcc caggctttga gcttcactgg
     4261 cgatgatctt ggcagcattg acatcatcgt ccagaattaa aagagtgcat tcacgcattg
     4321 ctccacctcc gtgcttgatc tgttgaattt gctggataga tctcctgttt ggctcatcac
     4381 attcgccgac tggataaaac aaagcgtgag gcatttcacc actttcagga cggtaggcct
     4441 caatccagaa tgctagaaag attgatgtta atataaattt tgtaaaattt atatagcctt
     4501 attgggctcg ggcgtgaatg gctgcttccc aggtatcttg tgtatgcagc cagggctcac
     4561 agcactcacg caacccgttg gcatgatgtt agtgagtcgg gatgtttgcg cggctgaggt
     4621 agggcaatac ctgttcttta ggcatgggtt tggcaaaata gtatccttgc acccattggc
     4681 agcccatggc ttgcaagaac ctgacctggc tttcagtttc tgcaccctcg gccacacatt
     4741 ccatatgcat gccctggcat aacgccaaga tggagcgcac gatggtttgg ctggtggtat
     4801 tggtttcaat atccttgatg aagctgcgat ctatcttgag tttgtccagt ggcaagtgct
     4861 gcacatgact caagctggag tagccggtgc cgaagtcatc cagagcgacc ttggcaccag
     4921 cttgccgcag taggccaata ttttcccggg cggtttcaaa gtcttgtagc aaggcggttt
     4981 cagtgatttc aaatacaaac ctgccaggct gtaactggct ggaatgcatg atgctgatta
     5041 agtctttaat caccgcctgg ttggtgacat cataagcaga caagttaaag gaaaggcgca
     5101 tcgttgccgg ccaggtgagc atttcgctga tggagcggtt aaacatcaat ttggtgattt
     5161 cggcaatcat acccagactt tccgccaccg gaataaactg ggctggagac accatgccaa
     5221 tcgacgggct gttccagcgg gccaggctct caaacgcgcc cacttcacct gcatcaatgt
     5281 caataatcgg ctggaatacc gggaatagct cggtctggat atctgcacta cgcaaggcct
     5341 ggtcgatgcg attcaagtgc atgcgttctt tttccagtac ttcggaataa atttccaggt
     5401 tgccgcgctc ggtacgtttg acgtgataca aggcaaagtc cgcatgctca tacaagtctt
     5461 gtgcagaggt gaattcgtca cacacaatgc atgcacccat agaggcttta atgacaatca
     5521 gcagcccatc aatgctgatc ggttgcgaaa tcgcctggtt gatttcatag gcgatctgct
     5581 tgagcgatcc atactcatca tctgtcacca ggtgaaaagc aaactcgtcc ccacccaggc
     5641 ggtaaaaacg cacttcgttc ggccttacct gtgccagtcg tttaccgact tctaccaata
     5701 ccaggtcacc aatatgatga ccgtgcacat cattcacagg tttaaagtta tccagatcca
     5761 gaatgccgac cgcaaaacat tggctggttt gccgggcgag aagcagctct tgttccactt
     5821 gtacaaaaaa ctggcggcga ttgggtaatt gggtcagcac atcctgattg gccaggcgct
     5881 ggttatcctt taaaagggcc tgcgtttcat acgtagcatt cacccattgg tcaaataccg
     5941 tactgctggc gtgcatggcc agcaacatgc cgacctcaaa taccataacc agtaaggcga
     6001 ccacgtaggc gtgctctatc tggccgatca tgacgcaggc aatcgccgaa gagatcatca
     6061 gcaggttata aatataggcg gcagtaccga tttttctcag gataaatgca aag
//
LOCUS       DATMSK010000050        10730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_450917,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000050 DATMSK010000000
VERSION     DATMSK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450917"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..495)
                     /locus_tag="VNZ84_01695"
     CDS             complement(28..495)
                     /locus_tag="VNZ84_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HWU65692.1"
                     /translation="MLEVLIFIYQNYWDKHDASELKETDETIMAYELSQAGFNHQDIL
                     HAVDWVKDLRRSVQQPQYQQDPAAIRIFCDIERSKINDESLNFLGLLQRSDIISAYER
                     DLIIDRAMVLPQETLSMEDIRWITMMVLWDETRKQDYLFVEDALFNPQGLSLQ"
     gene            complement(559..1671)
                     /gene="dprA"
                     /locus_tag="VNZ84_01700"
     CDS             complement(559..1671)
                     /gene="dprA"
                     /locus_tag="VNZ84_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWU65693.1"
                     /translation="MTTAIEQCTPWIALSLVNGLGSVTYCQLLLNFKTPEAILAAPYS
                     ALREVVSHDVADGIQRALDDSHIALTLEWLGQADNHLITLGDPYYPQRLLETDQPPPV
                     LYAKGNLQVLKRPGLAIVGSRHATPQGESTAENFAESLCRAGFAVVSGLALGIDGAAH
                     RGALKADGATIAVVGTGLDIVYPARHKSLAHQVAQYGLLLSEYPLGTPSLTHNFPRRN
                     RIISGLSEGCLVVEANIDSGSLITARLAIEQGREVFAIPGSIHSPVAKGCHALIKQGA
                     KLVESTEDIVSELRHVRLPNPAFSISPNGPLPDWTNLPAEASPVLACMGFDPIFAEQI
                     AARAGLTPEQVSTMLTLLELEGAVSHLANGQFQRLA"
     gene            complement(1690..2724)
                     /locus_tag="VNZ84_01705"
     CDS             complement(1690..2724)
                     /locus_tag="VNZ84_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWU65694.1"
                     /translation="MTKIVQIITSLALACSSLLVQAEEIPLRPQHPDRHVVVKGDTLW
                     DISAKFLKNPWQWPQIWQLNRQRIKNPHWIYPGDVIVLDTSTGKPVLKLVRESVTLEP
                     GVIVTPIKGDAIPAIQPSTILPFLTKPMLISPEELKAAPRIIAAQEEREILSPGTHIY
                     VQGLPESARNVWAIYREGEPVKDPETGELLGNEAHYLGEARLLRPGQPATLTVTQAKE
                     EIAVKDKLIAVEDELIPAYMPHAPEMQIQGQIARVSEGINETGYGRVVVLNRGEEQGL
                     ERGHVLSVLRKGANMIDPESDPKKPVSIQLPDEPVGLVMVFKTFPKLSYALVMQASQP
                     IHTEDVVRTP"
     gene            2893..3408
                     /gene="def"
                     /locus_tag="VNZ84_01710"
     CDS             2893..3408
                     /gene="def"
                     /locus_tag="VNZ84_01710"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWU65695.1"
                     /translation="MALLPILQYPDPRLHTVAKPVQVFDEPLQQLVSDMAETMYDAPG
                     IGLAATQVDHHIQLIVIDLSKEKDDLLVLINPKIIAQSGSQDYEEGCLSVPGVYESVT
                     RAEFITVEAQDVHGKTFKLDADGLLSVCIQHEMDHLKGKVFVEYLSPLKRNRIRTKMV
                     KHAREIERNSY"
     gene            3427..4347
                     /gene="fmt"
                     /locus_tag="VNZ84_01715"
     CDS             3427..4347
                     /gene="fmt"
                     /locus_tag="VNZ84_01715"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWU65696.1"
                     /translation="MRIIYAGTPEFAVPALQGLMDAGHDIVMVLTQPDRPAGRGMQLK
                     ASPVKQLALEHGLRVFQPESLKPAEVQDEIAAVAADAMIVAAYGLIIPTAVLNIPKHG
                     CYNIHGSLLPRWRGAAPIHRAILAGDAETGVTIMEVVPKLDAGNMISKWAVPITEADT
                     TQSLHDAISREGARLMVEAMQTLQATGQLPSEVQDESLVTYAHKLEKSESAIDWTQPV
                     QQLSRQVRAFNPFPVATAKFKEQVCKVWFAQMAVGQGKPGVVIETHPLRVACGKGALE
                     IHELQMPGGKRQTAQQFVQGQHVQVGDQFF"
     gene            4500..5372
                     /gene="htpX"
                     /locus_tag="VNZ84_01720"
     CDS             4500..5372
                     /gene="htpX"
                     /locus_tag="VNZ84_01720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HWU65697.1"
                     /translation="MLGNWTKTFVLMAAITALFVAVGGALGGQGGMLLALLLAGGMNF
                     YAYWFSDQAVLKMYGAVEINAENNFGNGQFRNYYNMVNELADRAELPMPRVFVINESQ
                     PNAFATGRNPENAAVAATTGIMQILSERELRGVMAHELAHVKNRDTLISTISATVAGA
                     ISSIAQFGLFFGGGHREGEDRPNPLIGILIMVLAPLAASLIQMAISRSREYEADRMGA
                     DISRDPKALAAALEKIHNYAHQIPNMTAEQHPETGQMMIINPLSGADLAGLFSTHPQT
                     EERIRRLLALANQY"
     gene            5488..6777
                     /gene="rsmB"
                     /locus_tag="VNZ84_01725"
     CDS             5488..6777
                     /gene="rsmB"
                     /locus_tag="VNZ84_01725"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HWU65698.1"
                     /translation="MQVAQLIAAYAVAEVLAGHNLTISMPKALRKVKSATPQQQAAAQ
                     DYSYLTLRFYAEAAAYLKALTPKPVTDDRVKALLLVALSQLLHSRDDDFTVVNQAVEA
                     AGKTGQRWAKGLVNAVLRNFLRQRSALQSNLAQREEVIFNYPQWWIDTLKQQYPQHWQ
                     TILEVGNSHPPMTLRVNLRKTEVDAYLQLLQDSGIQASHTGGTAIQLQQPLPVNKLPK
                     FDAGWVSVQDAGAQWAAPLLDLQPGMHVLDACSAPGGKTCHILETCEVELLSLDVETE
                     RLQRVKDNLQRLSLKANVVVGSAAQSDWFNGQLFDRILADVPCSASGIVRRHVDMKWL
                     RRPQDLSTFAKTQQEILSNLWQMLAKDGKLLYVTCSIFQQENEMQIQQFLSTHADATR
                     LPVAFDVSTLPCQQQLGGQLLPTTEHDGLFYALLQKH"
     gene            6755..7330
                     /locus_tag="VNZ84_01730"
     CDS             6755..7330
                     /locus_tag="VNZ84_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HWU65699.1"
                     /translation="MRYCKNINHWFWLSALLGLAAWVAVAAGNHAHIRSAELVLRDDV
                     WTLDADIDIQLGRSLEEALNKGVALTFLYEFQIVKPLKYWFDDEVVTERRTVTLNYHA
                     LTKQYLLNYPQQQKVFDSLGDARRELGLIHDWRLVPHATLEKNQSYQAALKMHLDTSK
                     LPKALQLDALGESEWELVTPTFTWWVKEALR"
     gene            7335..9497
                     /locus_tag="VNZ84_01735"
     CDS             7335..9497
                     /locus_tag="VNZ84_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU65700.1"
                     /translation="MRYVLILSIVLAAVLLYLLSLASANTAISGDYYLVLLYINAGLA
                     LLLLGVIAYQLRHLYRTTKARQVGSKLNFRLLVTFALMALIPGMIVYLVSVNFLSRSI
                     ESWFNVKVESALDGGLNLGQRALDIMLADVKEKAQSMAVSLSLQPARSHYSQINDLRE
                     KAGIQDAALLSPQGAILAVSSSDPSSFLPELPSVPQLRQARSGIYGKIEPIQGKGLYM
                     RVLTPVEPLGITGELRILQLMQPVPKALSDTAEAVQDVYREYQTLSYSRQSLKDIFML
                     TLTMILLLSMLGALTIAFALSRRMAQPLTVLAEGTRLIATGDYSQQLPSFGKDELSVL
                     VKSFNSMTRQLQEAKNASEQSRQHVESARAYLEAILSHLSSGVMTINERAELRVYNLA
                     ATNILGISLVGFKNKIFTEIAAENPYLSPFTSVVMQHYEETLQFERHERHLTSQIEIE
                     GMHGTQILMLRVTRLPEGAGHGLVVVFDDVTAMAQAQRDAAWAEVARRLAHEIKNPLT
                     PIQLSAERLQFKLQDKLDSKDAEMLFKATDTIVNQVQAMKNMVNEFSEYARTPAVKLV
                     KLDLNALIRDVSSLYNASTAKLVLALHEQLPEIDADATMMRQILHNLLQNAQDALVNV
                     TEPQIVILTDYDGERIKLSVTDNGSGFPTDVMARVFEPYVTTKSHGTGLGLAIVKKMI
                     EEQRGQIRIDNLPQGGASVTISLPVDKQQQRRRVERIV"
     gene            9507..>10730
                     /locus_tag="VNZ84_01740"
     CDS             9507..>10730
                     /locus_tag="VNZ84_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWU65701.1"
                     /translation="MASRNILVVDDEIGIRELLSDILRDEGHIVRLAENAQIARDERL
                     KLRPDLVLLDIWMPDCDGISLLKEWANAGLLTMPVIMMSGHGTIDTAVEATRLGAFDF
                     LEKPIALQKLLKTVGAAIKHTEHLAHTEINLSSFGKSAVIQELKQRLEQLARHKNTLF
                     LIGKKGGSVPLCAKFLRPHNTPWVALTEYEKLVTAPMEILEQAREGVVFVDEVTKLGK
                     TEQKGLSLLINKSEKYGVRVICASEHGLPQLIEKAMLEQSLLQTLAGAAVKVPCLDEH
                     KEDIPDLATAMATLMVEASKIDYKAFDVAALNALRNADWPGDLDELESVVQNLLMTSL
                     GETITQADVQRVLANFQGQATESAEQHMPLNLDQPLREARDEFERFYFQYHMRLVKNN
                     MSRLSEISGLERTHLY"
BASE COUNT         2514 a         2579 c         2984 g         2653 t
ORIGIN      
        1 ggccgcgttt ttttcattcg tcatttatta ttgcagagat aggccttgcg gattaaacaa
       61 ggcatcttcc acaaacaggt aatcctgctt gcgggtctca tcccataaca ccatcatggt
      121 gatccagcgg atatcttcca tgctcagtgt ttcctgtggc agcaccatgg cgcgatcaat
      181 aatcaggtcg cgttcataag ccgaaataat gtcacttcgt tgcagcaagc ctaaaaagtt
      241 caggctttca tcattaatct tgctgcgctc aatatcgcaa aagatacgga tggccgcagg
      301 atcctgctga tattgtggtt gctgaaccga gcggcgcaga tctttcaccc aatccacggc
      361 atgcaaaata tcctggtggt taaatccagc ctgggacaat tcgtacgcca taatggtctc
      421 gtctgtttct ttcagttcag acgcatcatg tttgtcccag taattttgat aaataaatat
      481 caatacttcc aacatgtaaa tagtctcctc aagcacgctt tcatctacca gcgcttgtgg
      541 gtgagtctaa gtcaatcttt aagccagtcg ttgaaattga ccgttggcaa gatggctgac
      601 tgcaccttct aattcaagta gtgttagcat ggtggaaact tgttcgggcg tcaagcctgc
      661 acgggccgca atctgttctg caaagatagg gtcaaacccc atgcaggcca agactggact
      721 tgcttcggca ggcagattag tccaatcggg taatggccca ttcgggctaa tcgaaaatgc
      781 cggattcggc agtcgcacgt gacgtaactc actcacgatg tcttcagtgg attcaaccag
      841 ttttgcccct tgcttgatca atgcgtggca acctttggcc accggagagt gaatggagcc
      901 cggaatggca aagacttcac ggccttgttc gatagccagc ctggcggtaa ttaatgagcc
      961 gctgtcaatg ttggcttcta cgaccaggca gccttctgac aaaccgctga taatgcggtt
     1021 ccgccgtgga aagttgtgtg tgagtgaagg ggtgcccagc ggatattcgg aaaggagtag
     1081 tccgtattgg gccacctggt gtgccaggga tttgtgcctg gccgggtaaa cgatatccag
     1141 cccggtccct accacggcaa tcgtggcacc atccgctttc aaggcgccac gatgtgcggc
     1201 gccatctata cctagtgcca gtccgctgac cacggcaaac ccagcccggc acaggctttc
     1261 ggcgaagttc tcagctgtag actccccctg cggcgtggca tgccggctac ctacgatggc
     1321 caaaccaggg cgcttgagta cctgtaagtt tccctttgca tacaatacgg gtggtggctg
     1381 gtctgtttcc agtaagcgct gcggataata agggtcgccc aatgtaatca ggtggttatc
     1441 tgcttggccc agccactcca gggtcagggc aatatgcgaa tcatccagtg cgcgctggat
     1501 gccgtcggcg acatcatgac tcacgacctc acgcagggca gaatagggtg ctgccagaat
     1561 agcctctggc gttttaaagt ttaataataa ttggcaataa gtgacgctgc ccagcccgtt
     1621 gaccaggctg agcgcaatcc agggagtgca ctgttcgatg gcggttgtca cattcttgac
     1681 ggtgctatgt cagggggtgc ggacgacgtc ttcggtatgg atgggctgtg aggcttgcat
     1741 caccaaggca taggagagtt tagggaaggt cttgaatacc atgaccaggc caaccggttc
     1801 gtcaggcagt tgtatgctga ctggtttttt aggatcagac tccgggtcaa tcatattggc
     1861 tcccttgcgt agcactgaca agacatgacc acgctccagg ccctgttctt cgccgcggtt
     1921 aagtacgaca acgcggccat agccagtttc gttaatacct tcgctgaccc gtgcgatctg
     1981 gccctggatt tgcatctctg gcgcatgtgg catatacgct ggaatgagct cgtcttcaac
     2041 ggcaatcagt ttgtctttaa cggcaatttc ttctttggcc tgagtgaccg ttaacgtggc
     2101 gggctggcct ggacgcaata atctggcttc gccaagataa tgtgcctcgt taccaagcaa
     2161 ttcgccagtt tcagggtctt tgacgggctc gccttcacgg tagatagccc acacgtttct
     2221 agcggactct ggtaagcctt gtacataaat atgggttccc gggcttaaaa tctcgcgctc
     2281 ttcctgcgcc gcaataattc ttggcgctgc cttgagctct tccggtgaga tcagcatggg
     2341 tttggttaaa aatggcagga tggtactggg ttggatggct gggattgcat cacctttgat
     2401 gggtgtcaca attacgcctg gctccagcgt gactgattcg cgcacaagct tcagaaccgg
     2461 tttgcccgtg ctggtatcaa gcacaatgac atcgcctggg taaatccagt gcgggttttt
     2521 aatccgttga cggttaagtt gccagatttg tggccattgc caggggttct tgaggaattt
     2581 tgcagaaatg tcccatagcg tatcgccttt cacgacgaca tgacggtcag gatgttgcgg
     2641 gcgcaaggga atttcttcag cctgcaccaa caagctgcta caagccagcg caagcgaggt
     2701 tataatttga acaattttgg tcatgtggcg tctctgtagt atgcagctga aaaagatggc
     2761 agcactcatg gtgcgcagca tctgcgcaat gtcgaccaat ctagcatggg tttaaagttc
     2821 tgatcaaggt gaaaaagcgg gcgttttgcc ctgttttccc acaaactatt tgaatggaaa
     2881 gatttgtaga gcatggcact acttccaatt ttgcaatatc cagatccgcg cttgcacacg
     2941 gtggccaagc ccgtacaggt gtttgatgag ccattgcagc aactggtgag cgatatggcg
     3001 gagaccatgt atgacgcccc cggcatcggt ctggcagcga cacaggtaga tcatcatatc
     3061 cagttgattg tgattgattt gagcaaagaa aaagatgatt tgcttgtgct gattaacccc
     3121 aagatcattg cccagtctgg tagccaggac tatgaagaag gatgcttatc cgtgcccggt
     3181 gtctatgaga gtgtgacccg cgcggagttt attactgttg aagcacagga tgttcacggt
     3241 aaaaccttca agctggatgc cgatggtttg ctgagtgtct gtatccagca tgaaatggat
     3301 cacctcaaag gtaaagtatt tgtcgaatat ctgtcgccac tcaagcgtaa ccgtatccgc
     3361 accaaaatgg tcaaacatgc gcgcgaaatc gagcgcaata gttattaatt aagcacgggg
     3421 aatagcatgc gaattattta cgccggaacc ccggagtttg ccgtgcctgc tttgcaggga
     3481 ttgatggatg caggccatga cattgtcatg gtgctcaccc agccggaccg tcctgccggg
     3541 cgcggtatgc aactgaaagc cagtccagtg aaacagttgg ccttagaaca tggcttgcgc
     3601 gtatttcaac cggaaagcct gaaaccggct gaagtgcagg atgagattgc tgccgtggcg
     3661 gcggatgcca tgattgtagc ggcgtatggt ctcattatcc cgaccgcggt actcaatatt
     3721 cccaaacatg gctgttacaa cattcacggg tccttgttgc cacgctggcg tggggctgcg
     3781 cctatccatc gagcgatctt ggctggtgat gccgagactg gcgtcaccat tatggaggtg
     3841 gtgcccaagc tggatgccgg gaatatgatc agcaaatggg cggtgcccat cactgaggct
     3901 gataccacgc aaagcctgca tgatgcgatc agccgcgaag gcgcgcgctt gatggtagag
     3961 gccatgcaga ccttgcaggc gactggccag ttgccctctg aagtgcagga tgagtcgctg
     4021 gtgacctatg cgcacaaact cgagaaatca gaatctgcca tagattggac gcaacctgtg
     4081 cagcagctct cacgccaggt gcgcgcgttt aatcctttcc cagtcgccac agccaaattt
     4141 aaagagcagg tatgcaaggt atggtttgcc cagatggctg ttggtcaagg caagcctggc
     4201 gtggtaattg aaacgcatcc tttgcgcgtg gcttgtggca agggcgcatt ggaaattcat
     4261 gagttgcaaa tgcctggtgg caagcgacaa actgcacagc aatttgtgca ggggcagcat
     4321 gttcaggtgg gtgaccagtt tttttaaacg ccttctgcta gctatactga accagatacg
     4381 gcgctatttt ggcttacctc agtcattctt taagtgtctc tggatgggtt gtccattgaa
     4441 aaatccctgt catccccgca tattaaggtg gaagtattaa atgtattcaa ggagtatgta
     4501 tgttaggtaa ctggaccaaa acctttgtat tgatggcggc cattacggca ttgtttgttg
     4561 ctgtcggcgg ggcgctgggt ggccagggtg gtatgttgct ggcgttattg ctggccggtg
     4621 gcatgaactt ttatgcctac tggtttagcg atcaggcggt gctgaaaatg tatggtgcgg
     4681 ttgaaattaa tgccgagaac aatttcggca atggccagtt ccgtaattat tacaatatgg
     4741 tcaatgagct ggcggatcgt gctgagctac ccatgcctcg cgtatttgtg attaatgaaa
     4801 gccagcctaa tgcctttgca acaggccgta atcctgaaaa tgcggctgtc gcagcgacga
     4861 ccgggatcat gcaaattttg agtgagcgcg agttgcgcgg cgtcatggcg catgagttgg
     4921 cccacgtgaa aaaccgcgat acgctgattt cgaccatctc agcgactgtg gcaggggcga
     4981 tttcatccat tgcccagttc ggcctgtttt ttgggggtgg ccaccgtgag ggcgaggata
     5041 gacccaatcc attgattggt atcctgatca tggtgctggc acctttggct gcttcgctga
     5101 tccagatggc gatttcccgc tcgcgggaat atgaggcgga ccgtatgggg gcagatatca
     5161 gccgtgatcc taaagcgttg gcggcagcgt tggagaagat ccataactat gcgcatcaga
     5221 ttccgaacat gacggctgag cagcatcctg aaaccgggca gatgatgatt atcaatccat
     5281 tgtctggcgc tgacctggca gggttgttca gtactcatcc ccaaacggaa gagcgcatcc
     5341 gccgcttgct tgcattggca aatcagtatt aggttgcgct tgttacggta aaggctttct
     5401 acggaaagcc ttttttatgg cgcgcgtttt tcaatgtgta atcgcggcgc atcaagtaga
     5461 atagcttttt ttgcccagag tttacccttg caagttgccc agttgattgc cgcatatgcg
     5521 gtggccgaag tgttggccgg acataacctg actatttcca tgcccaaggc attgcgcaag
     5581 gtcaagtcgg caacgccgca gcaacaggcg gcggcgcagg attatagcta tctcacgcta
     5641 cgcttttatg cagaggctgc ggcgtatctt aaagccctca ccccaaaacc ggtgacggat
     5701 gatcgtgtga aggccttgtt gctagtggca ctgtcgcagt tgctgcatag tagggatgat
     5761 gattttaccg tggtcaatca ggcagtagag gcagcgggga aaaccggaca acgctgggca
     5821 aaagggttgg ttaatgctgt gttgcgtaac tttttgcgcc agcgttcagc cttgcaatct
     5881 aacctggctc agcgcgaaga agtcatattt aactatccgc aatggtggat agacaccctc
     5941 aagcaacaat atccccagca ttggcaaacc attcttgaag tcggtaatag tcatccacca
     6001 atgaccttgc gtgtgaacct gcgcaagaca gaggtggatg cttacctgca attactgcag
     6061 gacagtggca tacaggcaag ccatacaggt ggtacggcca ttcaattgca acaaccctta
     6121 cccgtaaata aattaccaaa gtttgatgct ggttgggtca gcgtccagga tgccggtgcg
     6181 caatgggcgg caccgttgct tgatttgcag cctggcatgc atgtgcttga tgcgtgcagt
     6241 gcgcctggcg gcaagacctg ccatatcctg gaaacctgtg aggtagagtt gctgtcgctg
     6301 gatgttgaga cggaacgctt gcagcgtgtt aaggataacc tgcagcgatt gagtctaaaa
     6361 gccaacgttg tggtcgggag cgcggcacag tctgactggt ttaatgggca attgtttgac
     6421 cgtatcctgg ctgatgtgcc ttgcagtgct tcagggattg tgcgccgcca tgtggatatg
     6481 aaatggttgc gacgcccaca ggatctgtcc acttttgcta aaacacagca ggaaatttta
     6541 tcgaatttgt ggcaaatgct ggcaaaagac ggtaaattgc tgtacgtgac ttgctcaata
     6601 ttccaacaag aaaacgaaat gcaaattcag caatttttaa gcacgcacgc agatgcgaca
     6661 agattgccag tcgcttttga tgtgtccaca ttgccgtgcc agcagcagct tggcggtcaa
     6721 ttattgccga ccaccgagca tgacggattg ttttatgcgt tactgcaaaa acattaatca
     6781 ctggttctgg ctatctgcct tgctgggtct ggcggcgtgg gttgccgtcg ctgcgggcaa
     6841 tcacgcccac attcgcagtg ctgagctggt gctacgcgat gacgtctgga cgctggatgc
     6901 tgatattgat atccaactgg gccgttcact cgaagaagcc ctcaataaag gggtggcgct
     6961 gactttcctt tatgaattcc agattgtgaa accgctcaag tattggtttg acgacgaagt
     7021 ggtgacagag cgccgtacgg tgacgctgaa ctatcatgcc ttgaccaagc agtacttgct
     7081 taattacccg cagcagcaaa aagtgttcga tagcctgggc gatgccaggc gcgagcttgg
     7141 cctgatccat gactggcgac tcgtgccaca tgccacgcta gagaaaaacc aatcttacca
     7201 ggcggccctc aaaatgcacc tggataccag caagctgccc aaagcgctgc aattggatgc
     7261 tctgggagaa agtgagtggg agttagtaac gcccacattt acctggtggg taaaggaagc
     7321 attgcgttag cagtatgcgt tatgtcttga ttttgtccat tgtgctggca gcagtcttgc
     7381 tttatctgct gtcgctggcc agtgccaata ctgcgatcag tggtgattat tacctggtgt
     7441 tgctgtatat caatgccggt cttgccctgc tcttattggg tgtgatcgct taccagctcc
     7501 gtcatctgta tcgcacaacc aaagccaggc aggtcggcag taagcttaac ttcagactgt
     7561 tggtcacctt tgcgctgatg gcgctcatcc cgggcatgat tgtatacctg gtgtctgtga
     7621 atttcctgtc gcgatccatt gagtcctggt ttaacgtcaa ggtggagtct gcccttgatg
     7681 gtggcctgaa ccttggacaa cgtgcgcttg atattatgct ggccgatgtc aaggaaaagg
     7741 cacaaagcat ggcggtttcg ctctctttac agcctgctcg ttcccattac agccagatta
     7801 atgatttacg cgaaaaggct ggtatccagg acgctgcctt gctcagtccg caaggggcga
     7861 ttttggcggt gagcagttct gaccctagca gtttcttgcc ggaattgcct tcggtaccgc
     7921 aattgcgcca ggccaggagt ggcatctatg gcaaaatcga accgattcag ggcaagggcc
     7981 tttatatgcg ggtgctgacg ccggtagagc ccttgggcat tactggcgaa ctacgcattt
     8041 tgcaattgat gcagccggtg cccaaggcgc tatcggatac tgctgaggct gtgcaggacg
     8101 tttaccgcga atatcaaacc ttgtcttaca gccgtcagtc cttgaaagat attttcatgc
     8161 tgacgctgac catgatttta ctgttgtcta tgttgggggc gttaacgatt gcgtttgccc
     8221 tgagccgccg catggcgcag ccacttaccg tgctggcgga agggacgcgg ctgattgcga
     8281 caggcgacta ttcgcaacaa ctcccttcgt ttggcaagga cgagctgagt gtgcttgtga
     8341 agtcgtttaa cagtatgacg cgccagttgc aggaggcgaa aaacgcctcc gagcaaagtc
     8401 gccagcatgt ggagtctgcg cgcgcctatc tggaagcgat tctgtcacac ctgagttccg
     8461 gggtcatgac catcaatgag cgtgccgagc tacgggtata taacctggct gcgacgaata
     8521 tcctgggcat ttcactggtt ggtttcaaga acaagatttt tactgagatt gcggccgaaa
     8581 acccttattt aagcccgttc accagtgtgg tgatgcagca ttatgaagaa accttgcagt
     8641 ttgaacgtca tgagcggcat ctaaccagcc agattgaaat agaaggcatg cacggcacac
     8701 aaatcctgat gctacgtgtg acccggttgc cggaaggcgc cgggcatgga ctggtggtgg
     8761 tgtttgatga cgtgactgca atggcacagg cccagcggga tgctgcctgg gccgaggtgg
     8821 cgagacgtct ggcgcatgag atcaagaatc cgttgacgcc tatccagttg tcggcagaac
     8881 gactgcagtt taaattgcag gacaagctcg atagcaaaga tgccgagatg ttgttcaagg
     8941 ccacagatac gattgttaac caggtacagg ccatgaaaaa catggtcaat gaatttagcg
     9001 agtacgcgag gaccccagcc gtcaagttgg tgaaactgga tttgaatgct ttaatcaggg
     9061 atgtcagcag cttgtacaat gcctcaactg ccaagctggt cttggccttg catgaacaat
     9121 tgccagagat tgatgctgat gcgaccatga tgcgccaaat cttgcataac ctgctgcaaa
     9181 atgcacagga cgcgctagta aatgtcactg aaccgcaaat tgttatcttg accgattatg
     9241 atggcgagcg tataaaactg tcagtgaccg ataacggcag tggtttccca actgatgtga
     9301 tggcgagggt gtttgaacct tatgtcacca ccaagtctca cgggacaggg ttgggcttgg
     9361 ccattgtaaa aaaaatgata gaagagcagc gtggacagat tcgcatagac aatctgccac
     9421 aggggggcgc cagcgtcacc atcagcttgc cggtagataa acaacaacag cgtcggcgcg
     9481 ttgagcgcat cgtgtaagga agtaccatgg cttcacgtaa tattttggtg gtcgatgacg
     9541 agataggcat ccgtgagttg ttgagcgaca tcttgcgcga tgaagggcat atcgtacgac
     9601 tggcggagaa tgcgcaaatt gcgcgtgacg aacgcctcaa gctgcgtccg gatctggtgt
     9661 tgctggatat ctggatgccg gattgcgacg gtatcagctt gcttaaagag tgggcgaatg
     9721 ccggtctgct gactatgccg gtgatcatga tgtcaggcca tggcacgata gatacagcgg
     9781 tggaagccac gcgcttggga gctttcgact tccttgagaa accgattgca ttgcaaaaac
     9841 tgctcaaaac ggtgggtgct gccatcaagc ataccgagca tctagcccat accgaaatca
     9901 acctgtccag ctttggtaaa agtgcagtga tccaggaact caaacagcgc ctggaacaac
     9961 ttgcccggca taaaaataca ttattcctga tcggtaaaaa gggcggtagt gtgcctctat
    10021 gtgccaaatt cctgcgacca cacaataccc cttgggttgc gctcaccgag tatgaaaaac
    10081 tggtgactgc gcccatggaa atcctggagc aggcgcgtga aggcgtggtc tttgtggatg
    10141 aagtgaccaa gctgggtaag actgagcaaa aaggcctaag cctgctgatt aacaagtcag
    10201 aaaagtatgg tgttcgggtg atttgcgcca gtgaacacgg cttgccgcag ttgattgaaa
    10261 aggcgatgct ggagcagtct ctgttacaga ccttggccgg cgcggcggtg aaagtcccat
    10321 gtctggatga gcacaaggag gatattcctg atttggcgac agccatggcg accttgatgg
    10381 tggaagccag caagatagac tataaagcct ttgatgtggc tgccttgaat gccttacgta
    10441 atgcggattg gcctggggat ctggatgagc tggaatctgt ggtgcagaac ctcctgatga
    10501 ccagccttgg cgaaacgatc acacaggcgg atgtgcagcg tgtgctggct aatttccagg
    10561 gccaggcgac ggagtctgcc gagcaacata tgccgctcaa cctggatcaa cccctgcgtg
    10621 aggcgcgtga cgaatttgaa cgtttttact ttcaatacca catgcgcctg gtcaagaata
    10681 atatgagccg cctgtcagag atttccggtc tggagcgcac gcatttgtac
//
LOCUS       DATMSK010000051         6200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_403765,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000051 DATMSK010000000
VERSION     DATMSK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403765"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /gene="wrbA"
                     /locus_tag="VNZ84_01745"
     CDS             <1..516
                     /gene="wrbA"
                     /locus_tag="VNZ84_01745"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HWU65702.1"
                     /translation="VESVADAKARVRTVPKVSANCEATEADIPSSGAPYASLQDIEEC
                     AGLALGSPTRFGNMAAPMKYFLDGTAGLWLKGSLIGKPAAVFTSSGSLHGGNEMTLMT
                     MMVPLMHHGMLMLGLPYSEPQLGTTKTGGTPYGASHIGGAMDDQPISEDEKKLCIALG
                     KRLAETALKLQ"
     gene            540..1265
                     /locus_tag="VNZ84_01750"
     CDS             540..1265
                     /locus_tag="VNZ84_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HWU65703.1"
                     /translation="MSAALAHEARQFLFSTRNAILSTHSMKYPGFPFGSVAPFVCNQQ
                     AEPIILISAIAEHTKNILENPKVSLLVFSGADDLQANARLTLMGEAIHCDKQDEDLRA
                     RYLRYLPQAAGYFDMHDFHFYRIQIEQVRYIAGFGRMGWFEGTSLNEGLAESMLAAQE
                     GGIVAHMNDDHKDSLLQYCRHVHGVTADHAEMVGLDADGFDVKFHQGEETEGLLRFKF
                     EQPVLNAMDARKALVALSQACKS"
     gene            1262..1624
                     /locus_tag="VNZ84_01755"
     CDS             1262..1624
                     /locus_tag="VNZ84_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HWU65704.1"
                     /translation="MIPRILFGSHMAASISLLGLIMLCLGWEILWAPLRVGGSLLFLK
                     TLPLLLPVFGILRGKRYTYQWSGMLILFYFTEGIVRSWSETGPSRWLAATEVLLSLVF
                     FASVIVYSKYTGRSYLSA"
     gene            1721..2470
                     /gene="surE"
                     /locus_tag="VNZ84_01760"
     CDS             1721..2470
                     /gene="surE"
                     /locus_tag="VNZ84_01760"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWU65705.1"
                     /translation="MRILLSNDDGYLAPGLNILATHLSKIADITVVAPERNRSGASNS
                     LTLDRPLSVKQAANGFFYVNGTPTDCVHIALTGLMQEMPDMVISGINDGANMGDDTIY
                     SGTVAAATEGYLLGIPSFAVSMSQHQPSHFETAAHIIVDLVRQYNHSEFTEPTLLNIN
                     VPDLPLQAIKGRQITRLGKRHKAEPVIQLQTPRGETVYWVGAAGQPNDGGEGTDFHAV
                     AQGYVSISPIQVDLTKHSQIATMQQWLQSKN"
     gene            2476..3150
                     /locus_tag="VNZ84_01765"
     CDS             2476..3150
                     /locus_tag="VNZ84_01765"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWU65706.1"
                     /translation="MAIPRTPSKTGIGMTSSRTRERMLSRLREQGIQDEVVIAAMADI
                     PRHIFVDEALSIRAYEDVSLPIGYGQTISQPYIVAKMTQILRNGKPLNKVLEIGTGCG
                     YQTAVLSRVSAEVYSLERIRPLVMKARNHLRTLKCTNVKLDHADGSQGLKDYGPFDAI
                     MVTAAASHVPQELVEQLAVGGRMVIPVGTAEQVLHLIERTTQGVQQTALEPVKFVPLL
                     GGTALS"
     gene            3219..4028
                     /locus_tag="VNZ84_01770"
     CDS             3219..4028
                     /locus_tag="VNZ84_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWU65707.1"
                     /translation="MPRAPVTTSPVSQENKPAAPTTIGSNQYLVQKGDTLYSIGRKFG
                     IPFLKLAEFNQLQAPYEIHVGQVLNLKTMSTGTTNSKVSETASATEDAEVVTSAITRP
                     EVAGTTDPSATSSAAANSAEPGKNAVNNDIEAIADSDIQWQWPLSGKVTTGFDAQTNK
                     GLNISGSSGQVISAAGAGKVIYSGMDVRGYGKLIIIKHNSNLLSVYAHQGNSLVKEGV
                     FVTEGEKLANLPAVANNKSPILHFEIRQKGKPVDPVGYLPNNQATISNSNT"
     gene            4098..4445
                     /locus_tag="VNZ84_01775"
     CDS             4098..4445
                     /locus_tag="VNZ84_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65708.1"
                     /translation="MDPNRYSKNRTIRPDSAWQIVQALFTLGLMVCGFIGIAVHVFGS
                     DQGPTDWLAWMAASPLNIALTVIGLVSAFAFHRYITHISTQQRRSASNLPVYAMMLLG
                     AYFIYQLISTGHW"
     gene            complement(4511..6196)
                     /gene="leuA"
                     /locus_tag="VNZ84_01780"
     CDS             complement(4511..6196)
                     /gene="leuA"
                     /locus_tag="VNZ84_01780"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWU65709.1"
                     /translation="MQTPSAHKYQAFPPINLTDRQWPNAVIRQAPIWMSTDLRDGNQS
                     LFEPMDADKKLRMFTMLCQMGFKEIEVAFPSASQTDFNFVRSLIEEKRIPEDVTIEVL
                     TQAREHLIRRTMESVRGARRVIVHIYNAISPTFREVVFNMSKEEVKTMAVDSVRLVKA
                     LAAEMPETEVIMQYSPETFTATELEFSKEVCDAVTEAWEANPVRKVIINLPATVEVAS
                     PNVYADQIEWMHRNLARRDSVILSLHPHNDRGTAVAATELGLMAGADRVEGCLFGHGE
                     RTGNVDLVTVALNMYTQGVHPGLNFSDMNAIGRTVEECTQIEIHPRHPYAGDLVFTAF
                     SGSHQDAIKKGFAMQQADARWNVPYLPIDPKDLGRNYDSIIRVNSQSGKGGVAYLMES
                     HYGVVMPRRMQVEFSGVVQQYTDQHGSEISHTELWTLFNQQYVSVETPIHVQDYQLET
                     HAQGQGICMQLVVSGIATIVSGYGNGPVDAAIHALQNIGVQVQVRSFEERSLSASTSA
                     GEAMACAFVEVVCQGRVCYGVGMDTNIVTASIKAIVSGTNRCSVQPMVMEMIA"
BASE COUNT         1584 a         1640 c         1508 g         1468 t
ORIGIN      
        1 gttgaaagtg tcgctgatgc aaaagcgaga gtgcgcacgg tacccaaagt ctctgccaac
       61 tgcgaagcga cagaggcgga catcccgtcc tctggtgcgc cttacgcctc attacaggat
      121 attgaagagt gcgctggact ggccctgggc agtcctaccc gttttggcaa catggctgcg
      181 cctatgaaat actttctaga tggtacggcg ggactctggc tcaaaggctc gctgattgga
      241 aaaccggcag cagtctttac ctctagtgga tcgctgcatg gtggtaacga aatgactctg
      301 atgacgatga tggttccatt gatgcatcac ggcatgctga tgctggggct accatactct
      361 gagccacaac tgggaaccac caaaacaggt ggcacgcctt acggtgccag tcatatcggt
      421 ggcgccatgg atgaccagcc gatttctgag gatgagaaaa aattgtgcat cgccctgggg
      481 aaacgcctgg ctgaaactgc actcaaactg caataaccgt ttttcaggaa aattaagtca
      541 tgtctgctgc cctcgcccac gaagcccgcc aattcttgtt ttcgacccgg aatgcaattc
      601 tatcgacgca ctcgatgaaa tatcctggct ttccattcgg ctctgtagcg ccatttgtct
      661 gcaaccagca agcagagccg attattctga tcagtgccat tgccgagcac acaaaaaaca
      721 ttcttgagaa tccaaaagtg tcattactgg tattttcggg agcagatgat ctgcaagcaa
      781 atgcccgatt aacactcatg ggagaagcca tccattgtga caagcaagat gaagacctgc
      841 gtgcccgcta cttgcgttat ttgccacagg ctgctggata ttttgatatg catgacttcc
      901 acttctaccg catccagatt gaacaggtac gctatatagc cggctttgga cgcatgggct
      961 ggtttgaagg cacttccctc aacgaaggtc tagctgaaag catgctagcc gcgcaagaag
     1021 gcgggatcgt tgcccacatg aatgatgatc ataaagatag tttgctgcaa tattgccggc
     1081 atgtacatgg tgtcaccgcc gaccatgctg aaatggtcgg cctggacgca gatggctttg
     1141 atgtgaaatt ccaccaaggt gaggaaaccg aaggcttgct acgctttaaa tttgagcagc
     1201 cggtattgaa tgccatggat gcgcgcaaag cactggtcgc cttatcgcag gcgtgcaagt
     1261 catgatcccg cgtattttat tcggcagtca catggcggcg agcatcagcc tgctcggact
     1321 gattatgcta tgcctgggct gggaaattct ctgggcgcca ctcagggtcg gtggcagcct
     1381 gctgttcctc aaaacgctgc cgctattgct acccgtcttt ggcattttgc gtggcaaacg
     1441 ttacacctat caatggtcag gcatgctgat tttgttttac ttcacagaag gtattgtcag
     1501 aagctggtcc gaaactggcc cttccagatg gctggcagca acggaggtct tgttgagcct
     1561 ggtatttttt gccagcgtga ttgtgtattc caaatatact ggtcgcagtt acctatcggc
     1621 ttgagatggc tgcaacaagg taaatgcgca gtcagtttca taataaattc tttcaagcac
     1681 gggcactgac aagcagagta aaccttatat aatattgcgc atgagaattc tgttatccaa
     1741 cgacgatggc tacctggccc caggcctcaa tatcctggcc acacatttaa gcaaaattgc
     1801 tgatattact gtggtggccc cagagcgtaa ccgcagtggc gccagtaact ccctcacact
     1861 ggacaggcca ctcagtgtta aacaggctgc caatggtttt ttctatgtga atggcacacc
     1921 taccgattgc gtccatattg ccctgactgg cttgatgcag gaaatgccgg acatggtcat
     1981 tagcggtatc aatgatggtg caaatatggg cgatgacacc atttactcgg gtacggttgc
     2041 agcagcgaca gaaggttacc tgcttggcat tccttctttt gcggtttcca tgtcgcaaca
     2101 tcagccatcc cattttgaaa ctgcagcaca cattatcgtc gacctggtca ggcaatataa
     2161 ccacagcgaa ttcactgaac caaccttgct caacatcaat gtacctgacc tcccgctgca
     2221 agccattaaa gggcgtcaaa ttacccgcct tggcaaacgg cacaaggccg agccagtaat
     2281 tcagctgcaa acgccacgcg gagaaaccgt atactgggtc ggggctgcag gccaacccaa
     2341 cgatgggggc gaaggaaccg attttcatgc tgtggcgcaa ggctatgttt ccatctcgcc
     2401 aatacaggta gacctgacca aacactccca gatcgcaacc atgcaacagt ggctgcaatc
     2461 caaaaattaa gtattatggc gattccaaga acaccaagta aaaccggcat aggcatgacc
     2521 tcttcacgca cacgcgagcg catgctctcc cgcctgcgtg aacaaggcat acaagatgaa
     2581 gtggtgattg ccgccatggc agatatcccc cgccatattt ttgtcgacga ggcgttatct
     2641 atccgggcct atgaagatgt ctcattaccg ataggctatg gacaaaccat ctcccagccc
     2701 tatatcgtcg ccaaaatgac acaaattttg agaaatggaa aacccctcaa caaagtgctt
     2761 gagataggga caggttgcgg ttatcaaacc gcggtattgt cgcgggtcag tgccgaagtt
     2821 tactccctgg agcgcatccg cccgctggta atgaaagcca ggaatcatct gcgcaccctg
     2881 aaatgcacca atgtcaaact ggatcacgcc gatggcagcc aaggcctcaa ggattacggc
     2941 ccgtttgatg ccatcatggt cacagcagcg gctagtcacg tgcctcaaga attagttgaa
     3001 cagctggctg taggcggccg tatggtgatt cccgtaggca cggctgaaca ggtattacat
     3061 ttaattgaac gcaccacgca aggtgtacaa caaacagcac tggaaccagt caaatttgtt
     3121 ccactgctgg gaggtacggc attatcatga gattacgcac tttgctaacg atatggggaa
     3181 ctgcctgtac acttgcaggc tgcgcgttaa actctcccat gccgcgcgca ccagtgacga
     3241 ccagccccgt cagtcaggaa aacaaacctg cggcacctac caccattggc tcaaaccagt
     3301 acctggtaca aaaaggcgat acgctttaca gcataggccg taaatttggt attcctttct
     3361 tgaagctggc agagtttaac cagttgcagg caccctatga aattcatgtt ggccaggtgt
     3421 taaatcttaa aaccatgagc actggcacaa ccaacagcaa agtaagtgaa actgccagcg
     3481 cgacggaaga tgctgaagtg gttacctcgg caatcacgcg ccctgaagtc gctggtacaa
     3541 ctgatccctc agcaacttcc agcgcggcag ccaattcggc agagcctggt aaaaatgcag
     3601 tgaacaacga tattgaggcg attgcagact ccgatattca atggcaatgg ccattatccg
     3661 gaaaagtcac aaccggtttt gatgcccaga ccaataaggg actcaacatt agcggcagtt
     3721 caggtcaggt gatcagtgct gctggcgctg gcaaagtcat ttatagtggg atggatgtgc
     3781 gcggctacgg caaactgatt atcatcaagc acaatagtaa tttgctttcc gtgtacgctc
     3841 atcagggcaa ctcgctggtg aaagaaggcg tattcgtgac agaaggtgaa aagcttgcca
     3901 atttgccggc cgttgccaat aataaatcac ccatattgca ttttgaaata agacaaaaag
     3961 gcaaaccagt ggacccggtt ggctacttgc ccaacaatca ggctacaatc agtaatagca
     4021 atacttaaat tggcataagg cctcaagtca ggccaatcgt ggatacgcaa gcattgcttg
     4081 cttgaaggga gcgctgtatg gacccaaatc gttacagcaa gaaccgaacg atccgtccgg
     4141 acagtgcctg gcaaatagta caggcgctgt ttacactggg attgatggtc tgtggcttta
     4201 taggcattgc cgtgcacgtg tttggcagtg accagggccc tacagactgg ttagcgtgga
     4261 tggcagcgtc ccctttaaat atcgcactga ccgtgattgg actagtcagt gcgtttgcgt
     4321 ttcaccgtta catcacccac atcagcactc aacagcgtcg ctcagccagc aacctgcccg
     4381 tctatgccat gatgttgcta ggggcttatt ttatttacca gctcatcagc acggggcact
     4441 ggtagcctaa gccctgtcat tcgaggtaac agggctaaac agtttcacag cacccagaac
     4501 cgcatagggt ttaagcaatc atctccatga ccataggttg cacactgcag cggttagtcc
     4561 cactcacaat agccttgata gaggcggtga cgatattggt atccatacct accccataac
     4621 aaactctccc ctgacaaacc acttcaacaa acgcacatgc catcgcctca ccggcagacg
     4681 tgcttgcact cagtgaacgc tcttcaaaac tgcgtacctg tacctgcacc cctatatttt
     4741 gcaaggcatg gattgcggca tccaccggtc cattaccata cccgctgaca atagttgcga
     4801 tcccgctgac tacaagctgc atgcaaatgc cctgcccttg agcatgcgtt tccagctggt
     4861 agtcctgaac atgtatgggc gtctcaacag acacatactg ctggttaaat agcgtccaga
     4921 gctcggtatg tgagatctcg ctaccatgct gatcagtata ttgctgcacg acaccagaga
     4981 actcgacctg catgcgtctt ggcatcacca cgccataatg actctccatt aagtatgcca
     5041 cgccaccttt tcctgactgg ctgttgacgc gaataatcga atcgtaattg cgacccaggt
     5101 ctttaggatc tatcggcagg taaggcacat tccagcgcgc atcagcctgt tgcatcgcga
     5161 agcctttttt aatggcatcc tgatgcgagc cggaaaatgc agtaaatacc agatcaccgg
     5221 cataaggatg acgcggatga atctcaatct gcgtacactc ctctaccgta cggccaatcg
     5281 cattcatgtc agaaaaattc agcccaggat gcacaccttg cgtgtacata ttcagcgcca
     5341 cagtgaccag gtctacattg cctgtgcgct caccatgccc aaacaggcac ccttccacgc
     5401 gatcagcccc ggccatgagc cccagctctg tagctgccac cgcagtccct ctgtcattgt
     5461 gtggatgcag gctgagaatc acactgtcac gtcgtgccag gttgcgatgc atccattcaa
     5521 tctggtctgc atacacattg ggactagcca cttctacagt tgctggcaga ttgataatga
     5581 ctttacgcac cgggttagct tcccaggcct cagtcaccgc atcacacacc tctttggaga
     5641 actcaagctc ggtcgccgta aatgtttcag gactatattg catgatgact tctgtttccg
     5701 gcatctctgc cgccagtgct ttcactagac gcacggagtc tacggccatt gtcttcacct
     5761 cttccttgct catattgaag accacttcac ggaatgtcgg tgaaatcgcg ttgtagatat
     5821 gtacgatgac gcgacgcgca ccgcgtacag attccatggt acggcgaatg aggtgttcac
     5881 gtgcctgcgt cagtacttct atcgtcacat cctcaggaat acgtttttcc tcgatcaggc
     5941 tgcgcacaaa attgaaatca gtttgcgagg cagaaggaaa agcgacctca atctctttaa
     6001 accccatctg gcacagcatg gtaaacatgc gaagcttttt gtcggcatcc ataggttcaa
     6061 acagtgactg gttaccatca cgcaaatcag tactcatcca gatcggtgcc tgacgaatca
     6121 ctgcgttggg ccattggcgg tcagttagat tgatgggagg gaaagcctgg tatttgtgtg
     6181 cggatggtgt ctgcatgttg
//
LOCUS       DATMSK010000052         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_263254,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000052 DATMSK010000000
VERSION     DATMSK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263254"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1090)
                     /gene="nadC"
                     /locus_tag="VNZ84_01785"
     CDS             complement(224..1090)
                     /gene="nadC"
                     /locus_tag="VNZ84_01785"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWU65710.1"
                     /translation="MTVSTLEYPSYLNASIIAEEVKTALAEDIGAGDLTAALIPAGTQ
                     ASATIISREEAVLCGCAWFEQCFKQLDPGTQLSWMVKDGDHVQANQPLVQIQGDARAM
                     LSAERPALNFLQTLSAVATHTRRYVDAIAGLPTQILDTRKTLPGLRLAQKYAVLTGGG
                     ANQRLALYDGILIKENHIAAAGSIAAVLATAFAMGSTSNIQIEVENLDELQQALDAGA
                     KSILLDNFTLNDLRQAVDINQKRALLEASGNVDLTTVRAIAETGVDRISIGALTKHIQ
                     AVDLSMRILMTF"
     gene            1248..1871
                     /locus_tag="VNZ84_01790"
     CDS             1248..1871
                     /locus_tag="VNZ84_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWU65711.1"
                     /translation="MQLLYTVNSPYARKVRIVAIEKHVDLTLTEVVLSAPDCPVSQHN
                     PLGKVPVLVLDDGDALYDSRVIVEYLDHRTPVAHLIPQDHSAKIKVRRWEALADGVCD
                     AAVAVMLEQRKPEAQQSVAWIEKQFAKVTAGLTALDDDLLKKKWCVNETFSLADIALG
                     TALGYLDLRFKNVQWEGKYWQEKFPNLAKHYGVLVKRPSFKQTMPQP"
BASE COUNT          490 a          479 c          495 g          536 t
ORIGIN      
        1 ctcacgcatt ttgtgagctg ccaggtcgag tttcgtgacc gcttgttttg gagagcaacg
       61 caggaatcaa gcggaagtta gggtgcattt cttttggtta cttattcttt gtgcaagcaa
      121 agaaaagtaa ctcgcctgag ggcgaaaaag atgcttgaaa tcaagcagga attgaaagaa
      181 gaaagccaat cggccgttac cgaatgaatt aattttttcc catctaaaat gtcatcagaa
      241 tccgcatact caaatccacc gcctgtatat gtttagtcag cgcgccaatc gaaatcctgt
      301 caacgccggt ttcggcaatc gcacggactg tcgttaaatc cacattacct gatgcttcca
      361 gcaaagcacg tttctggttg atatccacag cctgacgcaa gtcgttcaac gtgaagttat
      421 ccagcaaaat acttttggca cctgcatcca gcgcttgttg cagttcatcc aggttctcga
      481 cttcaatctg gatattgctt gtgctaccca tggcaaaggc agttgccaac acagcggcaa
      541 ttgatcctgc cgcagcgatg tggttttcct tgatcaggat gccatcgtac aaggccagtc
      601 tctggtttgc cccgccacca gtcagtaccg cgtatttttg cgcaaggcgt aacccgggca
      661 aggttttacg cgtatctaaa atctgtgtgg gcaagcctgc aatggcatct acataacggc
      721 gggtatgcgt cgccacagct gacaaggttt gcaggaagtt gagcgcgggc cgctccgcac
      781 taagcattgc acgggcatcg ccttgaatct ggaccagtgg ctggttggct tgtacgtggt
      841 caccatcttt caccatccag ctcaattgcg tgccagggtc aagttgcttg aagcattgtt
      901 cgaaccaggc acagccacaa agcacagctt cttcgcggct gatgatggta gccgatgcct
      961 gtgtgccagc tggaatcaat gccgcagtca ggtcgcctgc tcctatgtct tcagccaagg
     1021 cggtttttac ctcttcagca atgatgctgg catttaaata agaggggtac tcaagtgtgg
     1081 atacggtcat ttagttgtct gcaaggttaa atttatgcac gtgaccattc taagcccatg
     1141 ccaaaagcta ttataataaa actaccccaa ggcacgcgat tttggatatg cgatatttgt
     1201 ctggggtatt ttttcggaaa aatttaatct tctggctaac ctcacatatg cagcttttat
     1261 atacggtaaa cagtccttat gcgcgcaagg tgcgcattgt ggccatcgaa aaacatgtcg
     1321 atctcacgtt aaccgaggtg gtgttgtcgg cgccagattg cccagtcagc cagcataacc
     1381 ctttaggcaa agtgcctgtg ctggtgcttg atgatggtga tgcgttgtac gactcccgtg
     1441 tgattgtcga gtatctggat caccgtacac cggtggcaca cctgattcca caagatcact
     1501 ccgccaagat caaagtccgc cgctgggaag cattggcaga tggggtttgc gatgcagctg
     1561 ttgcagtgat gctggagcaa cgtaaaccgg aggcacagca gtctgtcgcc tggattgaaa
     1621 agcagtttgc caaggtgacg gccggattaa ctgcgctgga tgacgacctg ctcaaaaaaa
     1681 agtggtgtgt caatgaaacc tttagcttgg ccgacattgc actgggtacc gcattgggct
     1741 accttgattt gcggtttaaa aatgttcaat gggaaggtaa atactggcaa gaaaagtttc
     1801 ccaatttagc caagcattat ggcgtgttgg tcaaacgtcc cagtttcaag cagaccatgc
     1861 ctcaaccata attcgaattg acagaatgcc cacccagtga agtgggtatt ctgaataggc
     1921 cctttctctt tttttctgac gacaacagct aagctccgtc tcttcggttc ggttaagttg
     1981 ttgtttctgt tttattcttt
//
LOCUS       DATMSK010000053         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_28184,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000053 DATMSK010000000
VERSION     DATMSK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28184"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VNZ84_01795"
     CDS             <1..839
                     /locus_tag="VNZ84_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU65712.1"
                     /translation="VTGLLGMLLTACKPVTLLNAMIPKGQMEIERDVPFGALPRQKLD
                     IYQPRLLSGKPLPVVLFFYGGSWDSGSKQDYLFVAEALTAQGYLTVIADYRLYPEVKF
                     PQLMQDPALAFQWVKQHIAHYHGDPKQVFIMGHSAGAHLAVMLSLNAEYLAAVGLHPD
                     AIAGAVGLAGAYDFLPLSSSRLKAIFAPAAQEWISQPINFANGHNPPLLLLTGTADRT
                     VWPKNSINLAHTIQTKGGQVQLITYQGYDHVDMIAKLAKPLRGDRRLLEDIVGWLRFH
                     SG"
     gene            1365..>2368
                     /locus_tag="VNZ84_01800"
     CDS             1365..>2368
                     /locus_tag="VNZ84_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWU65713.1"
                     /translation="MTKLMIGLLLGCLSASLMAEPADALVQDLNSRVLRVQVGLKNGQ
                     YGLGSGVVVAENQVVTNCHVVANASSISVMSGGAAYHAVGVKPDWEHDVCMLQMEDLP
                     LAPVTMKSSRELKYEQPVFTIGYPSFVPAPVSTSGVVKGLFTMDDSVIVRATSTFRQG
                     ASGGGLFDDSGALVGLITLKSPGKDAYYYYMSVDWIQALISKPAGPVVVKSELPFWGK
                     PAAEWPYFMKVVYPYLHKDWKGLKVIAEQWAAKEPQNTEAWFYLAAAEYETQDFTIAE
                     QHLHRVLSMNDQHSQAFYYLGMIAEASGKHGEAMASVDALKQLDAEAAEALTVAMKSL
                     P"
BASE COUNT          554 a          548 c          641 g          625 t
ORIGIN      
        1 gggtcactgg cttgcttggg atgctgctaa cggcctgcaa gccggttaca ttactgaatg
       61 ccatgattcc caaagggcag atggaaattg agcgggatgt tccatttggc gcattgcccc
      121 ggcaaaaact ggacatctat caacctcgtt tactttctgg aaaacccttg ccggtggtct
      181 tgttttttta cggcggaagc tgggattccg gtagtaaaca ggactatcta tttgtggctg
      241 aggcgctgac tgcgcaggga tacctgacgg tgatcgccga ttatcggctt taccccgaag
      301 ttaagttccc gcagctgatg caggacccgg cactcgcatt ccaatgggtt aaacaacata
      361 tcgcccatta tcatggcgat ccaaaacagg tattcatcat gggccattct gccggggcgc
      421 acctggcagt catgctgtct ctcaatgcag agtacctggc agctgtcggg ttacacccgg
      481 acgcgattgc cggagctgtt gggctggctg gtgcctatga tttcttacct ttgagttcaa
      541 gccgcttgaa ggctattttt gctccagcag cgcaagaatg gatctcgcaa cctatcaatt
      601 ttgcaaatgg gcataatccg cccctgctgt tgctcactgg gaccgcagac cgcaccgtct
      661 ggccgaagaa ctcgattaat ttagcgcata ccattcaaac caagggtggc caggtgcagc
      721 tcatcactta tcaaggctat gatcatgtgg acatgattgc caaactggcc aagcccttgc
      781 gcggagatcg ccgtttactt gaagacatcg tgggctggtt acgctttcat agtggctagt
      841 cgcctctgat gttggcacac atcatttctt agaaggtagt cctaacctac cgcttaggtg
      901 ggcatgccct gaatttgctt aatctcgccg tctctccccg gcagctaaaa taccaagctt
      961 cttcgaatca ttaacctcgg catagagggc ggaaaaaagc tatttttcta tgccaatttt
     1021 ggtgtttggt cgttttgtcc gtgtctcaac gctgaaaacg cctcttgctt gtgcatctaa
     1081 gtgtagggga tgcacttttg tagtgcgttt tgggggtggt atggattcaa aatatacgct
     1141 tacggttcaa cggcccgcat gagtgtaaag gttgttttta atttaactat ttgattatta
     1201 atggatattt aacattttcg tgttgctttt tcatgcactt tttacgtgcg gtaaagcaag
     1261 ctccagggtt ggaatacgtc ttgctaaaac aagcttgaac agctttccaa cttacaaaat
     1321 gcctaacgga atattgccca aggcgccacg gattggagca cagcatgacg aagttaatga
     1381 tcggattatt gttaggatgc ttaagtgcat cgctgatggc ggagccagcc gacgctctgg
     1441 ttcaggattt gaacagtcgt gtgttgcgcg tgcaagtggg cctgaagaac ggtcaatatg
     1501 ggttgggctc tggcgtggtg gtcgctgaaa accaagtggt gactaactgc catgtggttg
     1561 ccaatgccag cagtatcagc gtgatgagtg gcggcgctgc ttaccatgcc gtcggcgtta
     1621 agccggattg ggagcatgat gtgtgcatgc tgcaaatgga agatttgcca ttggcacctg
     1681 tcaccatgaa atccagccgt gaacttaaat atgagcagcc tgtgtttact attggctatc
     1741 ctagctttgt acctgcaccg gtcagcacca gtggggtggt caaaggcttg tttaccatgg
     1801 atgactctgt cattgtgcgg gcgaccagta ccttcaggca gggcgctagt ggcggtgggc
     1861 tgtttgatga cagcggggca ttagtcggtt tgatcacgct caaaagccca ggcaaggatg
     1921 catattacta ctacatgtca gtggattgga tccaggcttt gatcagcaaa ccggcaggtc
     1981 ctgttgttgt gaaaagcgaa ttgcctttct ggggtaaacc tgctgccgag tggccttact
     2041 ttatgaaagt ggtgtatcca tacctgcata aagactggaa aggcctgaaa gtgattgccg
     2101 agcaatgggc ggcgaaagag ccacaaaaca ccgaggcctg gttttacctg gcggctgccg
     2161 aatatgaaac acaggacttc actattgccg aacaacatct gcaccgtgta ctcagtatga
     2221 acgaccaaca tagccaggct ttttactacc tgggtatgat tgcggaagcc agcggtaagc
     2281 atggtgaggc gatggcgagt gtagatgcac ttaaacaact ggatgcagaa gcggccgagg
     2341 ctttgacggt agcaatgaaa agcttgcc
//
LOCUS       DATMSK010000054         4816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_122331,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000054 DATMSK010000000
VERSION     DATMSK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122331"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1406
                     /locus_tag="VNZ84_01805"
     CDS             72..1406
                     /locus_tag="VNZ84_01805"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWU65714.1"
                     /translation="MTQSKQAKNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHN
                     AGHTLVVGQGTEQKVYKLNLVPSGIVREGVNCYIGNGVVLDTTHLLNEISTLEQGGLD
                     VKNRLKVSPGCPLILSHHVAVDLAREAKRSADKKIGTTGKGIGPAYEDKVARRALRVY
                     DLFYPERFAEKLREVMDYHNFVLTNYLGATAVDFQQQYDASMQHAAALKPMVADISAA
                     LYEANARGEHLLFEGAQGTLLDVDHGTYPYVTSSNCIAGQASAGTGVGANMLHYVLGI
                     TKAYTTRVGGGPFPSELDIETEGAPGYQMSNKGQEIGTVTRRKRRCGWFDAAALRRSA
                     RINGLTGLCITKLDVLDGIESLDICTGYKLDGKVVDMLPVGADDVARCEPIYETVPGW
                     TETTFGVKTWDALPKNAQHYLKRLEAVCGVPIAIVSTGPERDETIVLQHPFA"
     gene            1426..2013
                     /locus_tag="VNZ84_01810"
     CDS             1426..2013
                     /locus_tag="VNZ84_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HWU65715.1"
                     /translation="MRGQRLAWAITGSGHYLRESLAILQTLQDVDIYLSRAAAEIIQQ
                     YGFQSALEATGHKVYQDKTASSVPVERFYEGVYHTLVISPATSNTIAKMAYGFSDSLV
                     TNLFAQAGKTRVHSIVFACDTAPELESEAPRDNVVKVFPRQVDLENVAKLKQFADTSV
                     VEDMVTLQGAIMQRLAGLHDLHLHDGIQVLDHLTE"
     gene            2018..3454
                     /locus_tag="VNZ84_01815"
     CDS             2018..3454
                     /locus_tag="VNZ84_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HWU65716.1"
                     /translation="MNRLLFLTGKLAEHSLHQILQSIQADSKQSPFEYEVKQIGVSVA
                     ALMTPPLIARRLPQVNNVDKVILPGLCQGDLAPLEAQWGVPVERGPKDLKDLPQYFGQ
                     QGKAPDLTQHSVRIFAEIVDAPDLSIEKIVAKATHFKQQGADVIDLGCLPGKTFSHMT
                     DAVQALKAEGFNVSVDSMREDDLLAAGKAGADYLLSLHEKSLWIADEVPSTPILIPAK
                     PGSLASLQRAIEHCLKRGKAFIADPILDPIHFGLTDSIVRYHKLRKKYPDIAIMMGVG
                     NLTELTDADTTGVNAMLFGMISELNINAVLATSVSPHASEAVAEADVARRVMYRAQQD
                     KRLPRGYSNGLLGLHDRKPFPYTAQEIVEMAGQIKDPSFRIMVSEEGVHVYNRDGIQQ
                     DTDPYRFYAGLNVQEDASHAFYLGVELARAQIAWQLKKRYVQDELLEWGVASVQQNRQ
                     AKVAHREASIKERLEKTRNDKDGDPLSE"
     gene            3460..4035
                     /locus_tag="VNZ84_01820"
     CDS             3460..4035
                     /locus_tag="VNZ84_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HWU65717.1"
                     /translation="MIYETIVTTCSTEGEVHIAPFGIQWHDGRVLISPFRPSTTLQNI
                     LETGVAVLNLTDDVRVFAAAIARKTAFATEVVPNHQGVRLKGCLAHHVLSLQEIEEDE
                     VRPKLWMRVTESISHAPFMGFNRAQAAVIELAVLVSRLHMLPMEKITQEMQYLQIAID
                     KTAGPREQEAWQWLVDCINNHLAQQSGLQQA"
     gene            4032..4775
                     /locus_tag="VNZ84_01825"
     CDS             4032..4775
                     /locus_tag="VNZ84_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HWU65718.1"
                     /translation="MSQAMQVLVSVTSVAEAQLVLAAGVSLIDLKDTSHGALAALDIE
                     TSKAIARVVETYRQRSARTEIIISATVGDNCDTAADLMTLIDHRLQIGIEVIKLPEAI
                     WADPDYQSIIDGFLAKGIKMIAVLMPASLMAATLEKRLQSLAQQGYWGVMVDTMQKSS
                     TLVEMVAMPILASFVRTAQSLQLVVGVAGGLSLAHVASLSELGPDYLGFRSGVCADGQ
                     RSKGLLPERVHQLVSVACGNLLDNWSQTQ"
BASE COUNT         1192 a         1051 c         1340 g         1233 t
ORIGIN      
        1 atgggcactg gcaagtggtg gcggtgtaag tccgctcacc catttttgtt tttacaagat
       61 atttattgaa catgacacaa tctaagcaag caaaaaacgt cgttgtgatt ggtacccaat
      121 ggggcgacga aggtaaaggt aaaattgttg actggttaac agaccacgca caaggtgtgg
      181 tccgtttcca gggcggccat aatgccgggc atacactggt tgtcggccag ggcactgaac
      241 aaaaggtcta taaactcaac ctcgttccct ctggtattgt ccgtgaaggc gtcaattgct
      301 atatcggtaa cggtgtcgtg ctggatacca ctcatttgtt gaatgagatt tccacgctgg
      361 agcagggtgg tttggatgtt aaaaaccgct tgaaagtcag tcctggctgc ccattgattc
      421 tgtcacatca cgtggctgtt gatctggcgc gtgaagccaa acgcagtgcc gacaagaaaa
      481 ttgggacgac tggtaaaggt attggtccgg cttatgagga taaagttgcc cgccgtgcct
      541 tgcgcgtata tgacctgttc tatccagagc gctttgctga aaagctgcgt gaggtcatgg
      601 attaccacaa tttcgtgttg accaattacc tgggtgcgac tgcggttgat ttccagcaac
      661 agtatgatgc cagcatgcaa cacgcagctg cgttgaaacc tatggtggcc gatatttcag
      721 ccgctttgta tgaagccaat gcccgtggtg agcatttgtt gtttgaaggc gctcaaggca
      781 ctttactgga tgtagaccat ggtacttatc cttacgtgac ctccagtaac tgtatcgcgg
      841 gccaagcctc agccggaacc ggtgtcggtg ccaatatgct gcattacgta ctggggatta
      901 ccaaagccta cacaacacgt gtaggcggtg gcccgttccc gagcgagctg gatattgaaa
      961 cagaaggcgc acctggctac caaatgtcta acaagggcca ggaaatcggt accgtaaccc
     1021 gccgtaagcg ccgttgtggc tggtttgatg ccgctgcctt gcgccgatct gcccgtatta
     1081 atggcctgac aggattgtgc attaccaagc tggatgtgct ggatggcatc gagtcgctgg
     1141 atatctgtac tggttacaag ctggacggaa aagtggtcga catgctgcct gtgggggcgg
     1201 atgatgtggc gcgttgcgag ccgatttatg aaactgtccc cggctggact gaaaccacct
     1261 ttggcgttaa aacctgggat gcgttaccga aaaacgcgca gcattacctc aaacgcctgg
     1321 aagctgtctg cggtgtgccg attgccatcg tttccactgg accagagcgc gacgaaacca
     1381 tcgttctgca acatccattt gcttaatgga taagagtggt ctgagatgcg tggacaacga
     1441 ctagcctggg caattactgg ttccgggcat tatctgcgtg aatcgctggc aattttgcag
     1501 actttgcagg atgtcgatat ttacctgagc cgggctgctg ctgaaattat ccagcagtat
     1561 ggtttccagt ctgctttaga agccactggg cataaggtat accaggataa aacggctagc
     1621 agtgtgcctg ttgagcgttt ctatgaaggc gtttatcaca ccttggtgat ttcgcctgca
     1681 acctctaata ccatcgcgaa aatggcttat ggtttttccg atagcctggt gactaatctg
     1741 tttgcacaag caggtaaaac gagagtgcat agtatcgtat ttgcctgtga tactgcacca
     1801 gagttggagt ctgaagcacc cagggataac gtggttaaag tgtttccccg tcaggttgat
     1861 cttgagaatg ttgcaaaact aaagcagttt gcggatacca gtgtggtcga ggatatggtt
     1921 actttgcagg gggcgattat gcagcgcctg gcaggattgc atgatctgca tttgcacgat
     1981 ggtatacagg tgttggatca tctgaccgag taactccatg aatcgactgc tgtttcttac
     2041 cggaaaactg gcagaacaca gcctgcatca gatattgcag tccattcagg ccgacagtaa
     2101 acagtctcca tttgaatatg aagtcaaaca gattggagta tctgtcgctg cgttgatgac
     2161 cccacccctg attgcccgcc gtttgccaca agtgaataat gttgataagg tgattttgcc
     2221 ggggttgtgc cagggggatt tggcgcccct tgaggcgcag tggggggtac cagtggagcg
     2281 tggaccaaaa gacttaaaag atttgccgca atactttggc cagcaaggta aagccccgga
     2341 tttgacgcaa catagtgtca ggatatttgc tgaaattgtg gatgcgcctg atctcagtat
     2401 cgagaaaatt gtcgctaaag ccacccattt taagcagcag ggcgcagacg tgatcgactt
     2461 gggttgtttg cccggtaaaa ctttttcgca tatgacagat gcagtgcagg cattaaaagc
     2521 tgaaggtttt aatgtcagtg ttgactccat gcgcgaggac gatttactgg cagctggcaa
     2581 ggccggtgct gattatctgt taagcctgca tgaaaaaagc ttatggattg cagacgaagt
     2641 gccttcaacg cctatcctga tcccagccaa gcctggtagt ctggccagtt tgcaacgtgc
     2701 gatagagcac tgcctgaaac gtggcaaggc atttattgct gaccctattc ttgatcccat
     2761 tcatttcgga ttgactgact ctattgttcg ttatcacaag ctgcgcaaaa agtatcctga
     2821 cattgccatc atgatgggcg tcggtaacct gactgagtta actgatgcag acactacagg
     2881 cgtcaacgcc atgttgtttg gcatgatcag tgagttgaat atcaatgcgg tgctagcgac
     2941 ctcggtgagt ccgcacgcca gtgaagccgt ggcagaggcc gatgttgctc gccgcgtgat
     3001 gtatcgcgcc caacaggata aacgcttacc caggggatat tctaatggtt tgctggggct
     3061 gcatgaccga aagccattcc cgtatactgc gcaagagatt gttgagatgg ccggtcagat
     3121 caaggatccc agcttccgta tcatggtcag tgaagaaggc gtgcatgtct ataacaggga
     3181 tggtatccag caggatactg atccttaccg tttttacgct ggtctcaatg tccaggagga
     3241 tgcgtcgcac gcgttttacc tgggggttga attggcgcgg gcgcaaattg cctggcaatt
     3301 gaaaaagcgt tacgttcagg atgagttgct ggagtggggt gtggcctcgg tacagcaaaa
     3361 caggcaggca aaagtcgccc atcgcgaagc gtctatcaaa gagcgcctgg aaaaaacacg
     3421 taatgataaa gatggtgacc cactctcgga ataaccttaa tgatatatga aacgatagta
     3481 acgacctgtt caaccgaggg cgaggtgcat atcgcaccgt ttggcattca atggcatgac
     3541 ggcagggtat tgatttcccc tttccgtcct tctacaacgt tgcagaatat tctagagact
     3601 ggcgttgcag tattgaacct gacagatgat gtacgcgtat ttgcagctgc gattgcaaga
     3661 aaaacagcct ttgctaccga agtggtgcct aatcatcagg gtgttcggct taaaggttgt
     3721 ttagcgcatc atgtgttgag cttgcaggaa attgaagagg atgaggtgcg gcctaagtta
     3781 tggatgcggg tgacggaaag catatcgcat gcgccattca tgggatttaa ccgggcgcag
     3841 gcggcggtga ttgaactggc cgtgttggtc agccggttac atatgttgcc catggaaaaa
     3901 attacccagg aaatgcagta cctgcaaatc gcgattgata aaactgccgg cccacgcgaa
     3961 caagaggcct ggcaatggct ggtggattgc attaataatc atctggcaca acaaagtgga
     4021 ttgcaacagg catgagtcag gcaatgcagg tgttggtgag tgtgacttct gttgcagaag
     4081 cacaattggt attagcggca ggtgtgtcgc tgattgatct caaagatacc agtcatggtg
     4141 cattagccgc attggatatc gaaacttcaa aggcgattgc cagggtagtg gagacatatc
     4201 ggcagcgatc tgccaggacg gaaatcatca taagtgccac ggtaggagat aattgcgata
     4261 ctgcagcgga tttaatgact ttgatagatc atagactgca gattggtata gaggtgatta
     4321 aattacctga agcgatttgg gctgatccgg attatcaatc cattattgac ggcttccttg
     4381 caaaaggtat caagatgatt gcggtattga tgccagccag tttaatggct gcaacattgg
     4441 aaaagcgatt gcagtcattg gctcagcagg gatattgggg cgtgatggtc gataccatgc
     4501 aaaaatccag cactctggtg gaaatggttg ccatgccaat actggccagc ttcgtacgca
     4561 cagcgcagtc tttacaatta gtggtcggtg ttgccggtgg actgtcgttg gcgcatgtgg
     4621 cgtctctttc tgaattagga cctgattatc tgggattccg tagcggtgta tgtgcagatg
     4681 ggcagcggtc gaaaggtttg ctacctgaac gtgtacatca attagtatct gtggcttgtg
     4741 gaaatttgtt ggataattgg tcacaaaccc aataaatacc tgagtcaggc tgtgtttagg
     4801 gttgcgtcag cctgac
//
LOCUS       DATMSK010000055         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_122381,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000055 DATMSK010000000
VERSION     DATMSK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122381"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2257
                     /locus_tag="VNZ84_01830"
     CDS             <1..2257
                     /locus_tag="VNZ84_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HWU65719.1"
                     /translation="SLLSPKLLRKLWLILLVAMLGFGWAQARAWWRMHSALPVACEQQ
                     VIPVQGVIVSVPERDARGQHVDVAIERSFNASCPLPAKVRLHLYESSHRTQPAQSSAL
                     MPRLQAGERWQFSVRLKRPHATRNPHGFDYAAWCLANEIGASGSIVAKLPMVRLQPLV
                     WQPNALIARWRSRVGDRIERVLGKTPTSAVIRALVIGEDSQITRADWQLFVDTGINHL
                     VSISGLHITMLASLGYFCAGGLWRLRPNLALYLPSSLAASIGGAMVAVAYSVLAGFSI
                     PTQRTLYMLLTVLTMLSLRQRFPFSWILSIAIWVVLVLDPWAVLAPGFWLSFGAVAIL
                     AFAMGSRLRAVNGWQVAIHTQWVITLAFVPVLVMFFNQLSFVSPVANALAIPMVSLGI
                     VPLAIAGAILPFDFLLQLAAAIWETCAHALQWLRQLPWAVSYSATPSIWAWLMALLGT
                     LVWLMPRGWPLRWAGLLLWLPMFLPAPATLLPEQMRVTVLDVGQGLSVLVQTARHAML
                     YDAGPAYMADSDAGQRIVLPYLRHLGIRQLDLAMVSHDDNDHVGGMASVLAGVPARKL
                     LSSLAPDATFFNQLQHMKPPVVMPHEACHAGQQWQWDQVTFRVLYPAQGLPAGIKDND
                     KSCVIQMISAHGSLLLTGDIERYAEQALLASAPAGLTSHVMTMPHHGSKTSSSLAFVH
                     AVRPEIAIATMGYLNRFGHPKPEVVARYDALGAQTLRSDSDGAIVLDFTQGSRPRVIR
                     WRAIEPHYWEQ"
     gene            2358..>2840
                     /locus_tag="VNZ84_01835"
     CDS             2358..>2840
                     /locus_tag="VNZ84_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWU65720.1"
                     /translation="MWEIILAAGWPIWPLVIASVIALAIIVERFWALRASVVTPEQLL
                     PEVQNWLKQGGVTRETLSRLEAHSLLGKVFASALANASHSREVTKEAIEETGRAVAHK
                     LEQYLPTLGTIATVAPLLGLLGTVIGMVELFGSFTNSGHDVAQFARGISVALYNTAAG
                     I"
BASE COUNT          566 a          721 c          805 g          748 t
ORIGIN      
        1 ctccttactt tcccctaaac tgctacgcaa actgtggctg attttactgg ttgcgatgct
       61 gggctttggc tgggcgcagg cccgcgcctg gtggcgaatg cattcagctt tgcctgtcgc
      121 ctgcgaacag caagtgattc ctgtacaagg tgtgattgtc agcgtccctg agcgtgatgc
      181 acgcggtcag catgtggatg tggcgattga gcgaagcttt aatgcatcct gtccgctacc
      241 cgcaaaagtg cgtctccact tgtatgagtc ttcccatcgg acgcaaccag cgcaatcatc
      301 agccctgatg ccccgcttgc aagcaggtga gcgctggcaa ttcagtgtcc ggctcaaacg
      361 tccgcatgca acacgtaacc cgcatggctt tgattatgca gcctggtgtc ttgccaatga
      421 aataggtgcc agtggatcga ttgtggccaa attgcctatg gtccggctgc agccacttgt
      481 ctggcagcca aatgcattga ttgcacgttg gcgctccaga gtaggagatc ggattgagcg
      541 cgtgttgggg aagacaccta caagtgctgt catacgcgcg ctggtcattg gcgaagatag
      601 ccagatcaca cgggctgact ggcaactgtt tgtcgatacc gggattaatc atctcgtctc
      661 aatatctggt ttgcatatca ccatgcttgc ctcgttaggc tatttttgcg caggtggatt
      721 gtggcgtttg cggccaaacc ttgcactata tttaccatcc agcttggctg ctagcattgg
      781 cggggcaatg gttgcggtcg cttattctgt attggccgga ttttctattc ccactcagcg
      841 aacgctttac atgttgctca cggtgttgac catgttgagt ctcaggcaac gcttcccttt
      901 ttcatggata ctcagtatcg ctatctgggt cgtgttggta cttgacccct gggcggtact
      961 tgctccaggg ttctggctat cgtttggggc ggtagcgatt ctggcttttg ccatgggtag
     1021 ccgcttgcga gccgtcaatg gatggcaagt tgccatccac acgcaatggg taattaccct
     1081 ggcgtttgtg ccggtgcttg tcatgttttt caatcagttg tccttcgttt cacccgtggc
     1141 aaatgcattg gcgattccca tggtcagcct gggtatcgtg ccgttggcga ttgcgggtgc
     1201 aatattgccg ttcgatttcc tgctacagct ggcagcagca atatgggaaa cctgtgcgca
     1261 tgccttgcaa tggttgcggc aattgccttg ggccgtcagt tattcagcaa cgccttctat
     1321 ctgggcatgg ttgatggcct tgctgggtac tctggtatgg ttaatgccac gcggatggcc
     1381 attgcgttgg gcaggattgc tgttatggtt gcccatgttt ctacctgcgc ctgcaaccct
     1441 actgcctgag caaatgcgtg tgactgtgct ggatgttgga cagggactga gtgtgctcgt
     1501 gcaaacggca cgccatgcta tgttgtatga tgctggccct gcatacatgg cagacagcga
     1561 tgccgggcag cgtattgtct tgccttattt gcggcatttg ggcatccggc agttagacct
     1621 tgccatggtc tcgcatgatg ataatgacca tgtcggggga atggcgtctg tgctggcggg
     1681 agtgcctgcc aggaaattgt tgagttcact ggcacctgac gcaacttttt ttaatcagtt
     1741 gcagcatatg aaacctcctg tggtgatgcc gcacgaggct tgccatgccg gccagcagtg
     1801 gcaatgggac caggtcacgt ttcgtgtact ttatccagca cagggattgc cagccggtat
     1861 caaggataac gataaaagct gtgtgattca gatgatttca gcccatggga gcctattgtt
     1921 gactggggat attgaacgat atgccgaaca agcgctgcta gcctcggcgc cagcagggct
     1981 aacttctcat gtcatgacga tgccgcacca tggtagtaaa acctcgtcca gtctggcatt
     2041 tgttcatgca gtacgccccg agatcgctat cgccaccatg ggttatctga atcgttttgg
     2101 tcatcccaag cccgaagtgg tggcccgtta tgacgcgcta ggcgcacaga ccttacgttc
     2161 cgacagtgac ggagcaattg tgctggattt cactcaggga tcccgaccac gcgtcatccg
     2221 ctggcgagct atcgagccac attattggga acagtaaggc gtggtaattg taacctgacg
     2281 gataccgcac aaaacttgcc ctcaagcctg gttaacgcta aagtagcgga agtttaaaaa
     2341 caacaggagt gtttgctgtg tgggaaatca ttttagcggc cggttggcca atctggcctc
     2401 tggtgattgc ttccgtgatt gcgttggcaa ttatcgtcga gcgtttctgg gccttgcgcg
     2461 cctcggtcgt tactcctgaa caactattgc ccgaagtaca gaactggctc aagcaaggtg
     2521 gcgtgacgcg tgagactttg agtcgcctgg aagcacactc cctgttgggc aaagtctttg
     2581 ccagtgcctt ggcgaatgcc agtcactcac gtgaagtgac caaggaagcg atcgaggaaa
     2641 ccggccgtgc cgtagcgcac aagctggagc aatacctgcc aacactgggc accatcgcca
     2701 ccgtagcgcc attgctaggg ttgctgggta ctgtgatagg tatggtggaa ctattcggct
     2761 cattcaccaa tagcggccat gatgtggccc agtttgcacg cggtatttct gtagccttgt
     2821 ataacacggc agccggtatc
//
LOCUS       DATMSK010000056         9314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_75146,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000056 DATMSK010000000
VERSION     DATMSK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9314
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75146"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1322
                     /locus_tag="VNZ84_01840"
     CDS             75..1322
                     /locus_tag="VNZ84_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU65721.1"
                     /translation="MKIAIIGGGIAGNTLAYHLHQHHQMTLFEANDYIGGHTHTHAVE
                     IAGQTHAVDTGFIVFNDKTYPEFERILRETGVAWRDSSMSFSVRNEVTGLEYNGTSLN
                     SLFAQRRNLLSPAFYRMVRDILRFNKESLSLLTPGNEIALGDYLQQYGYSQQFIRDYI
                     VPMGAAIWSTDAEQMLQFPARFFVRFFHHHGMLTIDERPQWRTIVGGSSAYLQKVTAG
                     YREHIQLNTPVSSVQRQHDAVTVNTLNGKSEQFDYVFFACHSDEALKILGDQATSEEK
                     RVLGAIPYQDNTVYLHTDARLMPKRKLAWAAWNYHVTEKPSGKVQVTYNMNILQGIKS
                     AAPLLVTLNYTDAIDPAKVIKKLHYRHPRYTLEGTEAQTRHAEISGHHRTGYAGAYWR
                     NGFHEDGVVSALTAIQHFEKAMA"
     gene            1319..2113
                     /locus_tag="VNZ84_01845"
     CDS             1319..2113
                     /locus_tag="VNZ84_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HWU65722.1"
                     /translation="MTVDLTPTQKQPSQLHSATYTGTVSHQRLQPKLHGFKYKLFMLY
                     LDLEELPSLFENNRLWSYLKPNLAYFRRQDYLGPADKPLDICVRDLVEQHTGKRPLGR
                     IGLLTHCRYWGVCFNPVSFYYCHDMDGHLQAIVSHITNTPWQEQFAYVHDVKQDQSLL
                     FKLQKDFHVSPFMPMDIQYQWQFSTPAETLNVHMQNWQQDQAMFYATLNMQRQPWHAN
                     TLNRMLFAYPWMTLKVILAIYWQALKLWLKRIPFYPHPDQHSRIGD"
     gene            2116..3402
                     /locus_tag="VNZ84_01850"
     CDS             2116..3402
                     /locus_tag="VNZ84_01850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HWU65723.1"
                     /translation="MTTIPTTTSLVIDPLTKPLKSKSRARWVANLAKSQVLKRLQSLR
                     IGQLTLIDGASSHVFGQAETQAPTATITVLDPRFYGEIAFGGSIGAGEAYMLGYWQAD
                     SLTDVVRLMVLNQSVMDTLEGGYQWLSKPLLKVFHWLNSNSEAGSQKNIAAHYDLGNA
                     LFKLFLDPTMMYSSAIFDQDTASLQAASERKLQVICDKLALRPDDHVIEIGTGWGGFA
                     IYAAKHYGCHVTTTTISEQQYLLAKERVMAEGLQNQITLLKQDYRHLQGQYDKLVSIE
                     MIEAVGHQYYDTYFNKVSQLLKPDGVALIQAITIADQRYDSAIRSVDFIQRYIFPGSN
                     IPSNTAMLNSMTRASDLRLVDLQDIGLHYATTLRHWRENFFTNIEAVRQLGYSEAFIR
                     MWDFYLSYCEGGFAECALGDVHLLLAKPGYRIKQRV"
     gene            3433..5403
                     /gene="mnmC"
                     /locus_tag="VNZ84_01855"
     CDS             3433..5403
                     /gene="mnmC"
                     /locus_tag="VNZ84_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HWU65724.1"
                     /translation="MSTHAVLEWRDGLPYSTQFDDVYFSVSPDHPQHGLAETRYVFLQ
                     HNQLQQRWQQLPTDRPSHFVIIETGFGSGLNFLAALQLWRQTAPSKAHLHFVSIEIAP
                     FTLQDLHMAHAHFPELAELSQALCRQYRLLQPGINQLELSAFSTSLTLHIGDSAHILP
                     QLNLQADAWFLDGFAPAKNPQMWQPYLFECMARCANAGTTFATFTSAGLVKRGLQSVG
                     FEVQKTRGFGHKREMLWGRWSQNTQHVPNPAMPRVAVIGAGIAGCSSAWFLTNLGCKV
                     EVFERAATIASGASGNPRGMLYPRLNAEKPLNDQLAWRSYSFSLRHYANLDLGEDAFQ
                     ACGLVQLGASPREFKRVQKVAARYADLGLLKRLDAEQASAQAGVPVTTDSLFFEDGAW
                     VNPNVACASMLHHCNISLRTEHSITHLVKQASGWQLEINHSALSDYFDAVIVCNAAEA
                     SKLLPESGMWLNPVRGQMGMIAVQPAMNTLKTILCGDGYLTPQHQDQHAMGATFAPGD
                     HSTDIRQEDNLDNLDMVSRLSPAFKQANDAVITFARASLRCGSPDYLPYVGPVLAADA
                     LNTQPAPITRYTDLPPSKGLYAHVAHGSKGLMTAPYCAAMLARRLLTDFGHSLPQLTE
                     DSFWAGLHPQRHLFKQLGWQALLQPFSDKVDS"
     gene            5400..7172
                     /locus_tag="VNZ84_01860"
     CDS             5400..7172
                     /locus_tag="VNZ84_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWU65725.1"
                     /translation="MTPAIEALQKRLILSPQDPELHYELGRCYLGLKQADAAGEAFTK
                     ALQWAPGHPQILMQLGNVASLKGHETVAVDYFRQSLKQDPQQADVHFNLANSLRKLGE
                     TASAIQHYQQSLKLAPQDAECHNNLGNAYREQGQLALAVASYAEAIKLNPELVHAKVH
                     WIHQKQHMADWQGLDAAIAEVRQTLALNPRAQIPPFAFLAMPGTHDKEQLACASLWAQ
                     QHYGHIQPLPAAQPKSPNQRIKLAYISSDFRKHPLAYLVTEVIAAHDRKQFEVTLYSN
                     AKDDRSTEQLAFKAAADHWVDINQMPDAEVATHMRNAGIDILVDLTGYTQHSRCGVTA
                     YRPARLHVNWLGYPGSMGFINEKPLFDAIVVDKTLAEVALAERPVLLPCYQPNNAQRP
                     MATADTRAQHGLPEQGFVFCCLNQTFKITPDIFALWMRILRQVPGSVLWLLQSNSWAT
                     HNLQQHAQAAGIDPARLVFAARTSIEQHIARQQHADLMLDTSPYNAHTTASDALWQGI
                     PLLTVAGDTFPSRVSASLLNYIGLAECICADWDALEQRAIALANTPSASAALKARLKK
                     QAGVLFEPQVFCLQLEQAYRQLLS"
     gene            complement(7174..8085)
                     /locus_tag="VNZ84_01865"
     CDS             complement(7174..8085)
                     /locus_tag="VNZ84_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWU65726.1"
                     /translation="MSLSHKQKASRQKYTASLALIYGALCWGVIWYPYRLLNDAGLSG
                     VQSSLLSYSLALILGGMLFWRRLKFIPQIPVSAYWLSAVAGWTNLAYVLAVIDGEVMR
                     VMLLFYLSPLWTLLLARVWLKEPWHKPQLLAIILSLLGAILMLTNGQWIWPWPQSASD
                     WLALSAGIGFSITNVMTRFSTHLTIAAKGMLVWLGVFALALVVLLWQGGVNGLSLMHS
                     TLPGMDAFSLLLIIAVLLVSATLLVQYGVTKIPAIQASVLFMSELIVAAIAAYFLAHE
                     TMSLIEMVGGCCILAAGVLSVSAGESG"
     gene            8101..9291
                     /gene="dapC"
                     /locus_tag="VNZ84_01870"
     CDS             8101..9291
                     /gene="dapC"
                     /locus_tag="VNZ84_01870"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HWU65727.1"
                     /translation="MNPNLNLLQPYPFQRLRDLFQGLTPNPAFNHVNLSIGEPKHATP
                     TIISEALAKNLSGLASYPTTIGIPALREAINEWLQRRYKIPALNIDKAILPVNGSREA
                     LFAFAQAVIDTNVAEQPVVISPNPFYQIYEGAAFLASAQPYFVNTTPENDFAMDLTQV
                     PVDILKRTQLVFVCSPGNPSGKVMDIDAWKTLFELSDQYGFVIASDECYSEIYFDENH
                     PPVGALQAAHQLGRDFSRVVMFSSLSKRSNVPGMRSGFVAGDADIVEKFALYRTYHGC
                     AMNPAVQAASIVAWNDEAHVIHNRALYSEKFNAVTPVLQQVWHKTQLPDAAFYLWINT
                     EQDDTVVAKQLYQALNITVLPGSFLAREAHGINPGKGFIRMALVASTEETISAANRIK
                     QYIK"
BASE COUNT         2446 a         2464 c         2220 g         2184 t
ORIGIN      
        1 atggggctta tctggaacct taaacaggtg cctttggacg tccgcgaaca acatggcaat
       61 gtaagggatt aaccatgaaa attgccatta ttggcggtgg tatcgccggc aatacccttg
      121 cctatcatct gcaccaacac catcaaatga cgctatttga agccaatgat tatattggcg
      181 gccatacgca tacccatgca gttgaaatcg cgggtcaaac ccacgcggta gataccggtt
      241 tcattgtgtt taatgataaa acctacccgg agtttgagcg catcctgcgg gaaactggcg
      301 tagcctggcg cgatagcagc atgagcttca gcgtacgtaa cgaagtgacc ggcctagaat
      361 ataacggcac atccctgaac agcctgtttg cccagcggcg caacctgctc tcccccgctt
      421 tttatcgcat ggtgcgtgat attttgcgct ttaacaaaga atccctgagc ttgctgacgc
      481 ctggtaatga aattgcactg ggtgattacc tgcaacagta cggatattca caacaattta
      541 tccgtgacta tattgtcccc atgggcgcag ccatctggtc cactgatgcc gaacagatgt
      601 tgcaattccc tgcccgattt tttgtgcgtt tttttcacca ccacggcatg cttacgattg
      661 atgagagacc acagtggaga acaatcgttg gcggctcttc ggcttacctg cagaaagtaa
      721 ccgcaggcta tcgtgagcac attcaactca atacgccagt gagctccgta cagcgccagc
      781 atgatgcggt gaccgtcaat acactgaatg gcaagtcaga gcagtttgat tatgtatttt
      841 ttgcctgcca tagcgatgag gccttaaaaa tcctggggga tcaagccaca tccgaagaaa
      901 agcgggtatt gggtgccatc ccttatcagg acaatacggt gtacctgcac acggatgccc
      961 gtttgatgcc taaaagaaaa ctggcctggg ccgcctggaa ctatcatgtg accgaaaaac
     1021 ccagcggtaa agtacaggtc acctacaata tgaatatcct gcaaggaatc aagtctgcag
     1081 cgcccttact ggtgacgctt aattacacgg atgccatcga tccggcaaaa gtgatcaaaa
     1141 agcttcatta ccgccacccg cggtatacgc tagaaggtac agaggcacaa acccggcatg
     1201 ctgagattag cgggcaccac cgcaccggtt atgcaggtgc ttattggcgc aatggcttcc
     1261 atgaagatgg tgtcgtgagt gccctcactg caatccagca ctttgaaaag gccatggcat
     1321 gaccgtcgat ttaacgccaa cacaaaaaca gcctagccaa ctgcacagtg ccacttatac
     1381 cggcacggtc agccaccagc gcctgcaacc caagctgcat ggctttaagt acaagctgtt
     1441 tatgttgtac ctagatttgg aagaactgcc tagtttgttc gaaaacaatc gcttatggtc
     1501 atatcttaaa ccaaatttgg catatttcag gcggcaggat tatttaggtc ccgctgataa
     1561 accactggat atttgtgtac gtgacctggt ggaacaacac acaggtaagc ggccgctggg
     1621 ccgtataggc ctgctgacgc attgccgcta ttggggcgtg tgcttcaacc cggtgagctt
     1681 ttattactgc catgatatgg atggccactt acaagcaatc gtcagtcata tcaccaatac
     1741 gccctggcaa gagcagtttg cttatgtgca tgacgtcaaa caagatcagt ctttactgtt
     1801 taaattgcaa aaagactttc atgtctcccc gtttatgcct atggatatcc agtatcaatg
     1861 gcagttttca acgcccgctg aaaccttaaa tgtgcatatg caaaactggc agcaagacca
     1921 agccatgttt tatgccacgc tcaacatgca gcgccagccc tggcacgcca ataccctgaa
     1981 ccgcatgttg tttgcctacc catggatgac gctgaaagtg atcctggcga tttactggca
     2041 ggccctgaag ttatggctga aaagaattcc tttttaccct caccctgacc aacactctcg
     2101 cattggagat tgatcatgac gaccattcca accacaacat cattagtcat tgacccgcta
     2161 accaaaccac tcaaatccaa gagccgcgcc agatgggtag ccaatttagc caaatcgcaa
     2221 gtgcttaaac gactgcaaag cttgcgcata ggccagctta cgctgattga tggggcgtca
     2281 agccatgttt ttggacaagc cgaaacgcaa gcccctactg ccactattac cgtcctcgac
     2341 ccgcgttttt atggcgaaat cgcctttggt ggcagcatag gcgcagggga agcgtatatg
     2401 ttgggctatt ggcaggcaga ctcactgact gatgtggttc gcttgatggt gctcaatcag
     2461 tcggtgatgg atacacttga agggggatac cagtggctca gcaaaccttt attaaaagtt
     2521 ttccactggc taaacagtaa cagtgaagca ggcagccaga aaaatatcgc tgcacattac
     2581 gacctgggaa atgcgctatt caaattattc ctggatccaa ccatgatgta ctcctcggcg
     2641 atttttgacc aggacactgc cagtttgcaa gccgcctctg agcgaaaatt gcaagtgatc
     2701 tgtgacaaac tggctttaag acctgatgac catgtgattg aaatcggcac cggttggggt
     2761 ggattcgcca tttatgcggc taagcattac ggttgccatg tcacaacgac cacgatttcc
     2821 gaacaacaat atcttctggc aaaagaaagg gtgatggcgg agggcctgca aaaccagatc
     2881 acgctactta agcaggatta tcgccattta caggggcaat acgacaagct ggtctccata
     2941 gagatgatcg aagcggtggg ccatcaatac tatgacactt atttcaacaa ggtcagccag
     3001 ttattaaaac cggatggcgt agctttaatt caagccatca cgattgccga ccagcgatac
     3061 gacagcgcga tccgttcggt agactttatc cagcgttata tttttcccgg atccaacatc
     3121 ccgtcgaata cggccatgct gaacagcatg acccgcgcta gcgacctgcg cctggtcgat
     3181 ttgcaggata ttggattaca ttacgccacc accttgcgtc actggcggga aaacttcttt
     3241 accaatatcg aagccgtcag gcagttaggg tacagcgaag cttttatccg catgtgggat
     3301 ttttacctga gctattgtga aggcggcttt gcggaatgcg ccttaggcga tgtacatctg
     3361 ttactggcaa aacctggcta ccggatcaag cagcgcgtgt aacgctattg cgtgcatgat
     3421 aaacttgcgg ccatgagcac gcatgcagta ttggagtggc gggatggact gccctactcc
     3481 acacaattcg atgatgtcta tttttctgtc agcccggatc acccgcaaca tgggctggct
     3541 gaaacgcgct atgtgttttt acagcacaac cagttacagc aacgctggca gcagttaccc
     3601 accgaccgtc ccagccactt tgtgattatt gaaaccggtt ttggcagtgg gctgaacttc
     3661 cttgccgcct tacagctttg gcggcagacc gctccctcaa aggcccattt gcattttgtc
     3721 agtattgaaa tcgccccgtt caccttgcaa gacctgcaca tggctcacgc ccacttccct
     3781 gagcttgcag aactcagcca ggccttatgt cggcagtatc gcttattgca acctggcatc
     3841 aatcaactgg agctttcagc gttttcaacc agcttaacgc tacatattgg cgatagcgca
     3901 cacatattac cgcagttgaa tctccaagca gatgcctggt ttctcgatgg ttttgccccg
     3961 gcaaaaaacc ctcaaatgtg gcaaccttat ttatttgagt gcatggctcg atgtgccaat
     4021 gctggaacca cttttgcgac ttttaccagc gcgggattag tgaaaagagg cctgcaatcc
     4081 gttggatttg aagtgcaaaa aacaaggggg tttggtcaca aacgggaaat gttgtggggt
     4141 cgctggtcac aaaacaccca acatgtccct aatccggcaa tgccaagagt cgctgtgatt
     4201 ggtgcaggca ttgccggttg cagtagcgca tggttcctga cgaatttagg ctgcaaagtc
     4261 gaggtatttg agcgcgcagc aaccattgcc agcggcgctt ctggcaatcc acgcggcatg
     4321 ctctaccctc gcctgaacgc agaaaaacca ttgaatgacc aattagcctg gcgcagctac
     4381 agtttcagct tgcgccatta tgccaatctg gatttaggag aggatgcgtt tcaagcttgt
     4441 ggcctggtgc aactgggcgc tagtcctcgt gaatttaaac gggtgcaaaa ggttgccgct
     4501 cgttatgctg atctcggcct gctaaaaagg ttggatgccg agcaagcaag cgcccaggcg
     4561 ggtgtgcctg taacaacgga tagcttattc tttgaagatg gtgcctgggt taatccgaac
     4621 gtggcttgtg caagcatgct tcatcattgc aatatcagcc tgcgcactga acactccatc
     4681 acgcacctgg taaaacaggc ctctggctgg cagcttgaaa tcaatcacag cgcgctcagc
     4741 gactattttg acgccgtgat tgtatgcaat gcggccgaag caagtaaatt gctaccagag
     4801 agtggcatgt ggctgaatcc ggtacgtgga cagatgggga tgattgctgt acaacctgcc
     4861 atgaacacgc tcaaaaccat tctgtgtggg gacggctacc tgaccccgca acatcaggac
     4921 cagcatgcca tgggggctac gtttgcaccg ggcgaccaca gcaccgacat ccgccaggaa
     4981 gacaatcttg ataatctcga catggtcagc cgtttaagcc cagcgtttaa gcaggcaaat
     5041 gatgccgtca tcacctttgc ccgtgcctcg ttaagatgtg gatcgccaga ctatttacct
     5101 tatgtggggc ccgtactggc agcagatgcg ctcaacacac aacccgctcc catcacacgg
     5161 tatacggact tgccgccatc aaaagggcta tacgcacatg tagcccatgg cagcaaaggc
     5221 ctgatgactg ccccttattg cgccgcgatg ttggcaaggc gtttattaac cgactttggt
     5281 cattcgctac ctcaactgac tgaagacagt ttctgggcag gcttgcatcc acagcgccat
     5341 ttgttcaagc aattgggctg gcaagcatta ctacagccct ttagtgacaa ggttgactca
     5401 tgacacctgc cattgaagct ttacaaaaaa ggctgattct ttcgccacaa gatcctgagc
     5461 tacattatga attaggccgc tgctacctgg ggctcaaaca ggcagatgct gcgggtgagg
     5521 cttttactaa agcattacag tgggcaccag gtcatccgca gatcttgatg caactgggca
     5581 atgtggcaag ccttaaaggg catgaaaccg tggctgttga ctatttccgg cagtcattaa
     5641 aacaggatcc tcagcaggcc gacgtgcatt tcaacctggc taatagtctg cgcaaattgg
     5701 gtgaaactgc cagcgcgatt caacactacc aacagtcatt aaaactggca ccgcaagatg
     5761 cagaatgcca taacaacctc gggaacgctt atcgagaaca aggccaactg gcacttgcag
     5821 ttgcctctta tgcagaggcg atcaaactca accccgagct cgttcatgcc aaagtacact
     5881 ggatacatca aaagcaacat atggcagact ggcaagggtt ggatgcagcg attgctgaag
     5941 tgcgccaaac cttggcactg aatcctcgtg cccagattcc gccatttgcg tttctggcaa
     6001 tgccgggcac tcatgacaaa gagcagcttg cctgtgccag cttgtgggct caacagcatt
     6061 atggacatat ccaacctctg cctgcagcgc aaccaaagtc gcccaatcag cggatcaagc
     6121 tggcttatat ctcttcagac ttccgcaagc accccttggc ctatttagtg acagaggtca
     6181 ttgcggcgca tgatcgcaaa caatttgaag tcactttgta tagcaatgca aaagatgacc
     6241 gatccacaga gcagctggca ttcaaagcag ccgcggatca ttgggtagat atcaatcaaa
     6301 tgcctgatgc tgaagtggct acacatatgc gcaatgcagg tattgatatc ctggtcgacc
     6361 tcaccgggta tacacaacac agccgttgcg gcgtcaccgc ttaccgtcct gcccgcttgc
     6421 atgtgaactg gctcggatat cctggcagta tggggtttat caacgaaaaa ccattgtttg
     6481 atgctattgt tgttgataaa acactcgccg aagttgcatt ggctgagcgg ccggtgttac
     6541 tgccatgtta tcaaccgaac aatgcacaac gcccgatggc aactgcggat acgcgtgcgc
     6601 aacacggcct gcctgagcaa ggctttgttt tctgttgcct gaatcagaca ttcaaaatca
     6661 cgcctgatat ctttgccctt tggatgcgca tcctgcgcca ggttccaggc agcgtattat
     6721 ggttattaca aagcaattca tgggcaacac acaacctaca acagcatgca caagcggcgg
     6781 gcattgatcc tgcacggcta gtgtttgctg cgcgtaccag tatcgaacaa cacattgccc
     6841 ggcagcaaca tgctgacctg atgctggaca ccagtccata taatgcacac accactgcga
     6901 gtgatgcctt atggcaaggc atccccttac tcaccgttgc tggggacact ttcccttctc
     6961 gcgtcagtgc cagcttactc aattatattg ggctggctga atgtatttgt gcggactggg
     7021 acgcactgga acaacgtgcg attgcgctgg cgaatacccc ttctgcttcg gcagcactca
     7081 aagccagatt aaaaaagcag gcaggcgtat tattcgagcc tcaggttttt tgccttcagc
     7141 tggaacaggc ttaccggcaa ctcttatcct agtttagcca gactcaccgg cactgaccga
     7201 caacacacct gccgccaaga tacaacatcc gccaaccatc tcaatcaggg acatggtttc
     7261 gtgcgcaagg aaataagcag caatggcagc gacgatgagc tcggacataa ataatacgga
     7321 tgcctgaatg gcaggtattt ttgtgacgcc atattgaacc agcagtgtcg ctgaaaccag
     7381 tagcacagca atgatcagca acaggctaaa tgcatccata ccagggagcg tgctgtgcat
     7441 cagcgataat ccatttactc ccccctgcca tagcaagacc accaacgcca aagcgaacac
     7501 gccaagccaa accagcatgc cttttgctgc aatcgtgaga tgggtcgaga aacgtgtcat
     7561 cacattggtg atagagaagc ctatgccggc agacaacgcc agccagtctg aagcagattg
     7621 cggccatggc catatccatt gaccatttgt cagcatcaat attgcaccaa gcaaagacaa
     7681 aatgatcgcc agcaattgtg gtttatgcca tggttctttt aaccagaccc gcgccaataa
     7741 cagcgtccac agcggggaca agtaaaacag tagcatgacc cgcatcactt caccgtcaat
     7801 caccgccagc acataggcca gattggtcca gcctgccacg gcacttaacc agtatgctga
     7861 aaccggaatc tgcgggatga actttagacg tcgccagaac agcattccgc ccaatatcaa
     7921 tgccaggcta taacttaaca ggctggattg cacaccggat aaaccagcgt cgtttagcaa
     7981 acggtaggga taccagatca cgccccagca taatgcacca tagatcaatg ccagagaagc
     8041 cgtatatttt tggcgggaag ctttttgttt atgtgacagg ctcacttata atacacacct
     8101 atgaatccaa atttaaacct actccaaccc tacccatttc aacgcctgcg cgatcttttc
     8161 caggggttga caccgaaccc ggcattcaac catgtcaacc tttcgattgg agaacctaaa
     8221 cacgcgacgc ccaccatcat cagcgaagcg ctcgcaaaga acctgagtgg attggccagc
     8281 tatcctacca caatcggtat ccccgccttg cgtgaagcga tcaatgaatg gttacagcgg
     8341 cggtacaaaa taccagcact caatatcgac aaagccattt tgcccgtcaa tggtagccgt
     8401 gaagcactgt ttgcttttgc acaagcagtc attgatacca atgtagctga gcaaccagtg
     8461 gtcatttctc ccaacccgtt ttaccagatt tatgaaggag cggcattcct ggcaagcgca
     8521 cagccttact ttgtcaatac cacgccagag aatgacttcg ccatggacct gacccaggtg
     8581 ccagttgata tcctcaaacg cacccaatta gtatttgtgt gcagcccggg caacccttcc
     8641 ggcaaggtca tggatatcga cgcctggaaa acgctgtttg aactctctga ccagtatgga
     8701 tttgtcatag catcagacga atgctattca gaaatttatt ttgatgagaa tcatccgcca
     8761 gtgggggcat tgcaggcggc acaccagcta ggacgcgact ttagccgtgt cgtcatgttt
     8821 agctcactct ccaaacgctc caacgtgccc ggaatgcgct cgggatttgt ggcaggtgat
     8881 gctgacatcg ttgaaaagtt tgcgctttac cgcacttacc atggctgcgc catgaaccct
     8941 gccgtacagg cagccagtat cgtggcatgg aatgacgaag cgcatgtcat acataaccgt
     9001 gcgctttaca gcgaaaaatt taatgccgtg actccagtct tacaacaggt atggcacaaa
     9061 acccaattac ccgatgccgc attttacctg tggatcaata cagaacaaga cgatacagtg
     9121 gtcgctaagc aactgtatca agcgttgaat attactgtat tgccaggctc cttcctcgca
     9181 cgcgaggctc acggcatcaa tcctggcaaa ggatttattc gtatggcctt ggtcgcatcc
     9241 actgaagaaa ctatttctgc agcaaacagg atcaagcagt atattaagta gtccgataga
     9301 caactaaata aaaa
//
LOCUS       DATMSK010000057         7676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_427516,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000057 DATMSK010000000
VERSION     DATMSK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427516"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..667)
                     /locus_tag="VNZ84_01875"
     CDS             complement(29..667)
                     /locus_tag="VNZ84_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU65728.1"
                     /translation="MDTKIKVIIVDDHAILRSGIKQILVASGDIEVVGEAENVAQAIK
                     CVRELAADVMLLDISLPDKTGIEALKLIKRENAALNILMLSMYMEEQYAVRSIRSGAS
                     GYLCKHTASEELLTAIHTLAKGKKYITPNVAEILAEQVGTDHSIAPHEVLSDREFQVM
                     RLIASGLSVSEIADKLALSVKTVSMYRTRLLEKMHLKHNADITHYAIKNQLV"
     gene            complement(744..1892)
                     /locus_tag="VNZ84_01880"
     CDS             complement(744..1892)
                     /locus_tag="VNZ84_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWU65729.1"
                     /translation="MSTPFIPSPAVSSSVANTPADSVKDALHESERRFSAIVSSIPGL
                     VFQMHMTADAQIVFTYLSEGCEALLGIPAAALLKDAHTLFNAMEEHSASQFKQKLQKS
                     AKQHKRLDWEGRIWIADWQDMKWVNIRATVRDLGQGAVQWDGIMLNISQSKHEKQEIE
                     QARRDLQALTAHIHQVKEQERVSIAREIHDDLGGNLTAMKLGLSTIIQQIEEGQPVNL
                     EQANMLQAIIDQTFDAVHRISGNLRPNILDLGLVDALDWQVSQFKKQLGVSAIFVTDC
                     RDLECDPDESMALFRICQEALSNIAKYAQADQVSVELSLVDQTLLMTISDNGVGIAPA
                     DKIKPNSFGLRGMQERAAALGGECQIVAAANGSGTCIQVRAPFIAIAS"
     gene            complement(2006..3829)
                     /locus_tag="VNZ84_01885"
     CDS             complement(2006..3829)
                     /locus_tag="VNZ84_01885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWU65730.1"
                     /translation="MSVNGIPMTASEIARETIKQMAARRVEPTPDNYTQIYFEVAGKP
                     AKEDAASALRKALKQLPHDSLEQTNWINRWEKLLKQDNWQGLVELLTETMQQQLNHST
                     KWPKAIRTLLLGLDDKRSGVDATRKRETLERVLINFGNDETLADKLIGMANHWLPADA
                     RVEGVPLTDVANLSASEAPSSVVDAAAGEAASFPSDVQQFHGAFTILQTLLKQTLQLG
                     LIPRLEGYPDLQKDALALQESTERAKKLKEWEALAKSLKALLMRVEVIGAKEDDVRGD
                     IIDLLHLLLSNIGELVAEDSWLSGQVYAVQSIISGPLDRTKLKQAEKSLKEVVYKQSL
                     VKHSLIEARNSFKAMISTFIDRLKYMGEASELYSGKIESYAQELSKTDDLIKINELVN
                     HLMQDTSVMQTDIMRSRDDLLNQQRAASDAQLRMHKLQEELQQLSEVVRIDQLTGVLN
                     RRGMDEAFNTEIARHRRNGESLSVALLDIDNFKMLNDQHGHAAGDSALKHLAGVIKRA
                     VRPTDIVTRMGGEEFVVILPNTNLDEAVVTMARLQRSLTKEYFLGNNQKLLITFSAGV
                     ALFKVEDDVATILLRADQAMYLAKKSGKNRVMTEIDLQANP"
     gene            4007..4103
                     /gene="ffs"
                     /locus_tag="VNZ84_01890"
     ncRNA           4007..4103
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VNZ84_01890"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4233..6020
                     /gene="dnaX"
                     /locus_tag="VNZ84_01895"
     CDS             4233..6020
                     /gene="dnaX"
                     /locus_tag="VNZ84_01895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWU65731.1"
                     /translation="MSYQVLARKWRPKSFGSLVGQDHVVQALSNALSQQRLHHAYLFT
                     GTRGVGKTTLSRILAKSLNCATGITANPCGECEACRAIDIGRYVDYVEMDAASNRGIA
                     DMVSLLEQAIYAPSSGRFKVYMIDEVHMLTKEAFNAMLKTLEEPPAHVKFILATTDPQ
                     KVPVTILSRCLQFNLRQMASSTITSHLQDILGQEDIQFEPPALQLIARAANGSMRDAL
                     SLTDQAIAYGNQSVRESDVRSMLGAIDQSYLYDLLRALIANDAATLLNIAQQMESRSI
                     GFESALNDLAALLHLLAVVQYSPQAIADDHPERQTLLDLASQLSAEMLQLYYQIAILG
                     HRDIGLAPDAYAGFTMTLLRMLAFKADAMAPTAPVAGNGGSPQEGPSRGNVTGGGARA
                     ALAQSRQLSGQQPSFTGSRQAPAPMEPPPIAAEISPATASPAPATIEITTTAIPEIAQ
                     IPQQAFSQPVVAADNSSLAWDGNWRALVENHLSKLGLVRALAQNCELVNFDNDEITLR
                     ISEPHKHLASANYLEKLNAALNNHFGRRLKLNMTTGQEVNTPARQLAIEKAELQDQAT
                     QAIMDDRFVQGLMQEMGATIVPNSIKPLH"
     gene            6045..6353
                     /locus_tag="VNZ84_01900"
     CDS             6045..6353
                     /locus_tag="VNZ84_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWU65732.1"
                     /translation="MMKGGMGNMMKQAQMMQANMQKAQAELANTEVEGIASNGMVKIT
                     MTCSNDVKRVQIDDAAMDDKETLEDLILIALKDARTKAEATSAAKMGNIMPAGFKLPF
                     "
     gene            6367..6966
                     /gene="recR"
                     /locus_tag="VNZ84_01905"
     CDS             6367..6966
                     /gene="recR"
                     /locus_tag="VNZ84_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWU65733.1"
                     /translation="MNNPAVLEQLISALRCLPGVGPKSSTRMAYHLLQRDREGAKKLA
                     ASLQQALDKLAHCDYCNNFSEHTVCSLCQDNHRDKSALCVVEMPTDLMMLENTRSYQG
                     QYFVLMGKLSPMDGIGPKDIHLDKLLKRAQDGEIHEVILATNFTSEGEATAHYIGQML
                     KSRGIRVSRIARGLPMGGEIEYVDSGTLSTALLERKAVT"
     gene            7229..>7676
                     /locus_tag="VNZ84_01910"
     CDS             7229..>7676
                     /locus_tag="VNZ84_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWU65734.1"
                     /translation="MELFLHTFDADSHTNLESPHYSGINKTSFSYHTEAELLQILDQI
                     IAQESAHTLRDTWVLISEDNQPVAPVEALQATMSFNTFYARHKYPWLLSDLEPHVQMH
                     YQPIVDFGQGGKVFGFEALCRLKTPEGKWLNGMEAFALAAEVKRTHE"
BASE COUNT         1972 a         2047 c         1770 g         1887 t
ORIGIN      
        1 cgatcttttt tatttccggc caatcagact aaacgagctg gttcttgatg gcataatgcg
       61 taatatccgc attgtgtttc agatgcatct tctcaagtaa gcgcgtacga tacatactca
      121 ccgttttgac cgacaaagcc aatttatcgg caatttcact gacagaaagc ccggaagcaa
      181 tcaggcgcat gacctggaat tcacggtctg acaatacttc atgcggggca atgctatgat
      241 cggtccccac ctgctctgcc aggatttctg ccacattggg ggtaatgtat ttcttgccct
      301 tggccaaggt gtgaatagcc gttagcaact cttcagaggc ggtgtgtttg cataaataac
      361 cggatgctcc ggaacggatg gagcgcactg catattgctc ctccatatac atcgataaca
      421 tcaggatatt gagcgcagca ttttcacgct tgatcagctt gagtgcctca ataccggttt
      481 tatctggcaa tgaaatatcc agcagcatga catcagccgc caattcccgt acgcatttaa
      541 tggcctgcgc tacattctct gcctcaccga cgacttcaat gtcgccactc gcaactaata
      601 tctgcttgat accagagcgt aaaatagcgt ggtcatcgac aataatgact ttaattttag
      661 tatccaagct gtcccctcat atgccaatgt cgtattggtc ttatcatgca tacaatttat
      721 tgtaaaacgc ccaagccatt ctgtcacgat gcaatagcaa tgaatggcgc ccgtacctgg
      781 atacaggtac ctgaaccatt tgcagcagct acaatctgac actctccacc caatgctgct
      841 gcgcgctctt gcatcccgcg caaaccaaac gagttaggct tgattttatc tgcgggggcg
      901 attcccacgc cattatcgga aatggtcatc aacaaagttt gatcaaccag tgacagctca
      961 accgaaacct gatcggcttg tgcatacttg gcaatattgg aaagcgcttc ctggcaaata
     1021 cggaacaacg ccatcgactc atccgggtcg cattccagat cgcggcaatc ggtcacgaat
     1081 atggccgaaa cacctaattg ttttttgaat tggctgacct gccagtccag cgcatcaacc
     1141 aagcccagat ccaggatatt tgggcgcaag ttacctgaaa tcctatgcac agcatcaaag
     1201 gtttgatcaa taatggcctg cagcatattc gcctgttcga ggttcactgg ctggccttct
     1261 tcaatttgct ggataatggt agaaaggccc aacttcatgg cagtaagatt accgccaaga
     1321 tcatcgtgaa tttcacgcgc aatactgacg cgctcctgct ccttgacctg gtgaatatgt
     1381 gcggtcagtg cctgcaagtc gcgcctggcc tgctcgatct cctgcttctc gtgtttgctt
     1441 tggctgatat tcagcatgat gccatcccac tgcacagcgc cctgccccaa atcacgaaca
     1501 gtcgcccgga tattgaccca tttcatgtcc tgccaatcgg caatccatat cctgccctcc
     1561 caatccaggc gcttatgctg cttggcagac ttttgcagtt tttgcttgaa ctggctggca
     1621 gaatgttctt ccatcgcatt aaacaaagta tgcgcatctt tgagcaaagc cgcggcagga
     1681 atccccagca aagcttcaca tccctcactc agataagtaa atacaatttg cgcatccgct
     1741 gtcatatgca tttggaacac cagcccggga atgctggaaa caatcgctga aaatctgcgc
     1801 tcgctttcat gtaaggcatc cttgacacta tcagcaggtg tgttggcgac agatgaactt
     1861 acagcaggtg aagggataaa gggagtggac aattggcacc tcaatcaaga tgctaattag
     1921 catagcatgc caaagcgcgg ctgggtatgc ctgctaccgt tttatgaggc aggtgcttga
     1981 atacggtgac gtacttgcaa aatagttacg ggttggcttg caggtctatt tcagtcatca
     2041 cgcggttttt accactcttt ttagccaggt acattgcctg gtcagcccgc agcaagatcg
     2101 ttgccacgtc atcctcaacc ttaaacagcg ctacaccggc actaaaagtg atcaaaagtt
     2161 tctggttatt gcccaggaag tattccttgg tcagcgagcg ctgtaagcgc gccatggtca
     2221 ccaccgcttc gtccagatta gtattaggca gtatgaccac aaactcctca ccccccattc
     2281 tggtcacaat atccgttggc ctgacggcac gtttgatgac gccagccaga tgcttgagag
     2341 cagaatcccc cgcagcatgg ccatgttggt cattgagcat cttgaaatta tcaatatcaa
     2401 gcaaagcgac actgagtgac tcaccattac gacggtgacg ggcaatttcg gtattgaagg
     2461 cttcatccat gccgcgccgg ttaagcacgc ctgtcaattg gtcaatccgc acgacttcac
     2521 tcaattgctg caactcttct tgcaacttat gcatccgcag ctgggcatca cttgctgcac
     2581 gctgctggtt aagcaaatca tcacgcgaac gcatgatatc cgtctgcatg acggaagtat
     2641 cttgcatcag atggttgacc aactcattga ttttaatcag gtcgtcagtt tttgatagct
     2701 cttgggcata gctttcaatt tttccgctat aaagctcact ggcctcgccc atatatttca
     2761 gcctatcaat aaacgtacta atcatggctt taaacgaatt ccgggcttca atcaggctat
     2821 gtttaactag gctttgttta tagacgactt ccttgaggct tttttctgcc tgcttgagct
     2881 tggttctatc caacggacca ctgataatgg attgcaccgc atatacctgc ccactcagcc
     2941 agctatcttc agccaccagt tcgccaatat tggaaagtag caaatgcaac aaatcgataa
     3001 tatcgccacg tacatcgtct tccttggcac cgatcacttc cacacgcatt agcaaggcct
     3061 ttagtgactt ggccaaagcc tcccactcct taagtttttt ggcacgctcc gtactttcct
     3121 gtaaagccaa tgcgtctttt tgcaaatctg gatagccttc caaccgcgga atcaatccta
     3181 gttgcaaagt ctgtttcagc aaggtttgca agatagtaaa agcgccatga aactgctgta
     3241 catcgctggg aaaactggct gcctccccgg cagccgcatc caccacgctc gatggtgctt
     3301 cggaggcaga caaattagca acatccgtta agggaacacc ttcaacacgc gcgtctgccg
     3361 gcaaccagtg atttgccatt ccgatcaact tatcagccaa ggtctcgtcg ttgccaaaat
     3421 tgattagcac acgctccagg gtctcgcgct ttctggtagc atccactcca ctacgcttat
     3481 catccagacc aagcaataag gtacggatcg ctttaggcca tttggtggag tgattgagct
     3541 gttgctgcat ggtctcagtc agtaattcaa ctaatccctg ccagttatcc tgcttgagca
     3601 gtttttccca acggttaatc cagttggttt gctccagaga atcatgtggc aactgtttaa
     3661 gcgctttgcg caaggcgcta gctgcatcct cctttgccgg tttacccgca acctcaaaat
     3721 aaatttgcgt gtaattgtcc ggtgtcggct ccacgcggcg ggcggccatt tgtttaatgg
     3781 tctcacgggc gatttccgag gcggtcatgg ggataccgtt aactgacata atcattacca
     3841 caatattggt tgaaaatacg gatcattatg caatgaaaga atcggcattt atgccactat
     3901 aagaaggtag attattggtc atttccaata cttcaatgca gctaggtaaa tcagccgtac
     3961 gaaaaaataa ggcagtgcaa cctgcttttt gcgttacaat agcgtccgcg ggcctccccg
     4021 cattgtaagg tagccaacct ggtcaggtgc ggaagcaagc agccacaact atcaagcaag
     4081 tgccgggggt tgggctcgcc cctttccatc ttgcctctcc gctgacgaaa tactcaacat
     4141 gccatcaatt agttgatatc aagtcgagca tgagctggca atttaagcta aagtacaggt
     4201 ttgccttata gccttaatca gtagagtgcg cgatgtctta ccaagtattg gcgcgtaaat
     4261 ggcgccctaa atcctttggc agtctggtcg gacaagacca tgttgttcag gcattaagca
     4321 atgccttgag ccagcagcgt ctgcaccatg cctatttgtt taccggaacc cgcggcgttg
     4381 gtaaaacaac actctcccgt atcctggcaa agtccctcaa ttgtgccaca ggcattaccg
     4441 caaacccatg tggtgaatgc gaagcttgcc gcgcgattga tattggccgc tatgtcgatt
     4501 atgttgagat ggacgcggct tctaaccgcg gcattgctga tatggtcagc ctgctggaac
     4561 aggccattta cgcaccatcc tccggccgct tcaaggtcta catgatagac gaagtgcata
     4621 tgctgaccaa agaagcgttt aatgcaatgc tgaaaacgct cgaagagcct ccagcgcacg
     4681 tcaaattcat actggcgacc actgatccgc aaaaagtccc ggtcactatc ctctcgcgct
     4741 gtttgcaatt caacttgcgc caaatggcga gcagcaccat tacctcccat ttacaagaca
     4801 ttctggggca ggaggatatt cagtttgaac ctcctgcgct gcaactgatt gcacgtgctg
     4861 ccaatggcag tatgcgtgat gccttatcac tgactgacca ggcaattgcc tacggcaacc
     4921 agtcggtacg tgagagtgat gtccgcagca tgctgggcgc aattgaccaa agctacctgt
     4981 atgacttact tcgcgcactg attgccaatg atgctgcgac cttgctgaat attgcccagc
     5041 aaatggaatc gcgcagtatc ggttttgaaa gcgccctgaa tgacctggcc gccttgctgc
     5101 acttgctagc cgtcgtgcaa tacagtccgc aggcgattgc cgatgaccat ccggaacggc
     5161 agacactact agacctggcg tctcaactga gcgcagagat gctgcagctg tattaccaga
     5221 ttgctatcct gggtcaccgt gatatcggcc tggccccaga tgcgtatgct gggttcacca
     5281 tgaccctgct gcgcatgttg gcatttaaag cagatgccat ggcgccgaca gcccccgtag
     5341 cgggcaatgg cggctcgcca caagaagggc cgtcacgagg caatgtgaca ggtggtggcg
     5401 cacgcgctgc acttgcacaa agccggcaat tgagcggtca gcagccatct ttcacaggaa
     5461 gcaggcaagc acctgcgcct atggagccac cgccaatagc cgctgaaata tctcccgcaa
     5521 cggcttcacc tgcgccagca acaatcgaga tcacaaccac ggcaatccct gagattgccc
     5581 agatcccgca acaggcgttt tcacaacctg tggtcgcagc tgacaactcg tcactggcat
     5641 gggatggtaa ctggcgcgca ctggtagaaa atcacttgag taaacttgga ttagtgcgtg
     5701 cgctggcaca gaattgtgaa ctggtgaact ttgataatga tgaaattacc ttacgcatca
     5761 gtgaaccaca taaacatcta gccagtgcca actacctgga aaagctcaat gctgcattga
     5821 ataatcattt tggccgtcga ttaaaattga atatgactac aggccaagaa gtgaacaccc
     5881 ccgccaggca gctagccatt gaaaaagccg aattgcagga tcaggccacc caggcgatta
     5941 tggacgaccg ctttgtacaa ggcctgatgc aggaaatggg cgcgaccatt gtgcccaact
     6001 cgattaaacc tctacattaa ccacagtaac cttaggagta gacaatgatg aaaggtggca
     6061 tgggcaacat gatgaaacag gcccagatga tgcaggctaa catgcagaaa gcacaagctg
     6121 aacttgcaaa cactgaagtc gaagggattg ccagcaacgg catggtaaag atcaccatga
     6181 cctgtagcaa cgatgtcaag cgcgtgcaaa ttgacgatgc ggccatggat gacaaggaaa
     6241 cactggagga tttgatttta attgccctca aagacgccag gaccaaagca gaagcaacca
     6301 gcgctgccaa aatgggtaat atcatgccag ctggctttaa actaccgttc taactgggac
     6361 tcaggtttga ataatccagc ggttctcgaa cagcttatta gtgccctgcg ctgcttgcca
     6421 ggagtggggc caaagtcatc cacacgaatg gcttatcatt tgctgcaacg tgatcgcgaa
     6481 ggcgcgaaaa agctcgccgc cagcctgcaa caggcgctcg ataaactggc acactgtgac
     6541 tactgcaata acttcagtga acatactgta tgcagtttgt gccaggataa ccaccgtgac
     6601 aaatccgctt tatgtgttgt ggagatgccc actgacctga tgatgctgga aaacacgcgt
     6661 agctatcagg gacaatattt cgtactcatg ggcaaactat ccccaatgga tggtattggc
     6721 cctaaagaca tccatctcga caagttgctg aagcgtgcgc aagatggcga gatccacgaa
     6781 gtcatactgg ccaccaactt tacttctgaa ggcgaagcca cggcccacta tatcggccag
     6841 atgctcaaat cccgtggcat ccgcgtcagc cgcattgccc gtggactgcc gatgggcggc
     6901 gaaatcgagt atgtggatag cggcacactg tcgactgctc tgctggaacg caaagccgtg
     6961 acttagccac gattacctcc aagctacttt ccatccatag tcagcgtacc ttattgacgt
     7021 attaacccat cccctctccc agttttccgc accaatttga tgcagacaat tagatggttt
     7081 cgtggctttt atgcccgctc atccagtaaa taaaccccac ttcctgtcgt tatagtaatc
     7141 atgtgatctg tcacggcagc catgcataaa atgcatggcg atgactgcgt ccgcctgcta
     7201 caaccctata aaagcaaagt gattgaccat ggaactattt ctgcatacct tcgacgctga
     7261 tagccacacc aacctggaaa gcccgcatta ttccggcatt aataaaacgt cattcagtta
     7321 tcacaccgaa gcggaactat tacagatact agaccagatc atcgcccagg aatcagcgca
     7381 tactcttaga gatacctggg tactgatttc tgaagacaat caacctgtcg ctcccgtgga
     7441 ggcattgcag gcaacgatgt ccttcaatac attctatgcc cgccacaaat atccctggct
     7501 cttgtctgac cttgagcctc atgtgcaaat gcactaccag cctattgtag attttggcca
     7561 aggtggcaag gtttttggct ttgaagcgct gtgtcgccta aaaacacctg aaggcaagtg
     7621 gttaaatggt atggaggcgt ttgcacttgc tgcagaggtc aagcgtactc acgaac
//
LOCUS       DATMSK010000058         9437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_5360, whole
            genome shotgun sequence.
ACCESSION   DATMSK010000058 DATMSK010000000
VERSION     DATMSK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5360"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1115
                     /locus_tag="VNZ84_01915"
     CDS             3..1115
                     /locus_tag="VNZ84_01915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWU65735.1"
                     /translation="MKLFPEPSDPDNVRYKVIFLNNVFTFAGLVSLTMSFFRWNSQPL
                     LGKIDLLFGCLAFALLWGLHRYKKNIELVSSIALWLCFVQFLTVFFIATDSASRSALL
                     LIILAAAFYLKGRVTGYYMLAILLILVVGNHMIQIFPSDYHHLDILSLCFYLLAQFFI
                     IRNYECLRESQTSHLKALNVNLEALVKQRTDQLAAANTALEFEKENLKRLSSTDHLTG
                     LSNRHHFETVFAEHTPVSHKKMTDALILIDIDHFKKINDTYGHVLGDTVIKAVAGCIK
                     KHSRISDITVRWGGDELIIYAPRTTLKQATRLAEKVRHQIHAMHLAGIGSISISAGVA
                     VLHPGDTLSQLLQRADHALYAAKQAGRNSVHAAALD"
     gene            1164..2153
                     /locus_tag="VNZ84_01920"
     CDS             1164..2153
                     /locus_tag="VNZ84_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65736.1"
                     /translation="MILRKAVAVLTFACLQLLCSPALHAEQWQTVPGREKFLTVSVDL
                     DSLQVGQHEDMIFFKLRADDHDDTIATRFVQASCSEFSMEVERESVFHKSTQTSEALD
                     WKGNPALTGHPEFAEARLSFWGVTQAYGQAISLACRRTGNRAPEQLQSLLPENCAVNH
                     PLRKVACANHASWRANYKLYFSRSHDVIHNCGISEERMTKQAAWLIKSVMRCKDEGCG
                     NEILENWIHKRGQDIANVIHMQKNTSTAWNASTQPAPLCESLNQADQAIASLQTEEIY
                     AKAKYLGCLKRETARLRATNTPAGTLAQQAEQLCEGPFQEWHDAISKQSAMRD"
     gene            complement(2186..2689)
                     /locus_tag="VNZ84_01925"
     CDS             complement(2186..2689)
                     /locus_tag="VNZ84_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HWU65737.1"
                     /translation="MCQWFNGRGGYVLGFLAGFGIVALALFIQVQNQLEPCPLCISQR
                     IVFMSLGALFLVHAFVSPQSWLHAGLALLEVLTALGGAGVAIRHWWLQAHKEEIIADC
                     GVGFDYMFENFPLKKALTLVFRGTGDCAAIDWTFLGLTLPQLGLISFILFGLYAVFMF
                     RARRCGS"
     gene            complement(2783..3787)
                     /locus_tag="VNZ84_01930"
     CDS             complement(2783..3787)
                     /locus_tag="VNZ84_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWU65738.1"
                     /translation="MSLKIHISLARSSFLLLALSCVCSPALSYADDEVTERALAEEVQ
                     AVSAGEDKPVLAPSEPLSSYSREQSVIRNDAYKQLIKLQVSLATGTKNALGLNSYEQA
                     HAHYCALAGESRDADAQFAMGWFYSMGKGVEVNHDVAARFFSLAAIQGHRDAMEWLDK
                     EPGNPELAKLPACMQKHAHSAAQILFRKRGPVYQLVKKHAPDYGVDVDFAMAVIAVES
                     GFNPNATSRKKAQGLMQLMPDTQARFHVKDAYDPEQNIKGGLSYLRWLIGYFKGDVEL
                     IAAAYNAGENAVLRYRGIPPYPETRDYVKRIAQYYKKKHHEVRQKSAMQQPNGTPASS
                     "
     gene            4003..5223
                     /gene="xseA"
                     /locus_tag="VNZ84_01935"
     CDS             4003..5223
                     /gene="xseA"
                     /locus_tag="VNZ84_01935"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWU65739.1"
                     /translation="MANLLSTSSKQILSVSDLTRLTKELLETSFPLFWINGEISNFTR
                     AASGHWYFSLKDDRAQVRCVMFKGRNSLIETMPREGDLIEARATVTLYEARGDFQLTI
                     EFLQPAGLGRLYEAFEQLKQRLQAQGLFDASRKKAIPANPQRIGVVTSPDAAALRDVL
                     TAIRRRYPGMGVIIYPTPVQGKGSAELIAQAIQLADQRQEVDTLLICRGGGSIEDLWS
                     FNEEIVAQAIAACCLPTISGVGHETDFTIADFVADLRAATPTAAAELACPDQSQLRQQ
                     VEQSRLRLMRAMQSLLRQHAQTLDYLSRRLVSPAQQLQQQKQVLQQWQHRLQLSMQQY
                     LQSHRRKLEHCATSLQQLNPHQVLARGYAIVQQQNGRAVTDTAQLTVGESLQLTLHRG
                     QAAVVVTQSPINKK"
     gene            5387..7267
                     /locus_tag="VNZ84_01940"
     CDS             5387..7267
                     /locus_tag="VNZ84_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HWU65740.1"
                     /translation="MDHTKNQAALHTMALAALGVVFGDIGTSPLYTMKEVFAVGHHPL
                     LLTQDNVYGILSLITWALLLIVSFKYVAFIMRADNRGEGGIMALLSLASRYAGGDPEQ
                     RKRIMMLGIIGACMFYADGMITPAISVLSAVEGLEVAAPQLDSWIVPITLCVLFGLFW
                     AQSKGTAVVGAFFGPIMLTWFAVLGILGVLNITHHPEILKALSPVYAFLFFYTQPKIA
                     FIALGAVVLAVTGAEALYADMGHFGRKPIRLAWFLFVLPALICNYFGQGALILAEPES
                     IQNPFYHLAPDWALFPLIILATLATVIASQAVITGAFSVSRQALQLGFIPRMHVSHTS
                     EQTEGQVYMPRVNWGLMVAVMGLVLIFGSSGDLAAAYGIAVTGDMVITTLLAAVVFHH
                     LWGWSKLKTATLITTFLVIDLSFFAANILKIPDGGWVPLLIGLIIFTLMRTWKTGRRL
                     LYGILKSESMELIPFIQAIGAHPPARVNGTGVFMTPNPDGVPHALLHNLKHNKVLHEN
                     MIILTVRFEDYPYTSQEERVSVEALPYGFYRVTIRYGYMDEPDLPRDLALTEPFGLSM
                     DTMDTSFFIGKEILIASDKPGMSFWRKKIFIGLFRTAETITNQFKLPPNRVVELGSQV
                     KI"
     gene            7331..8143
                     /locus_tag="VNZ84_01945"
     CDS             7331..8143
                     /locus_tag="VNZ84_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWU65741.1"
                     /translation="MFNIKTMTVAVMCALLSACASTTSNSVSGVQRSQFLLLPSATVD
                     KMSAQSYTQTLQDAQKKSTLNADKALLNRVTTISHRLIAQVGVFRPDATKWKWEVNVE
                     KNDQLNAYCMPGGKIMVFSGLADKLNATDDELAAVIGHEISHALREHGRERMSQAYVQ
                     QFGLQALAAYVGGTAGNMAAQGANMGSQLLFSLPNGREQEREADRMGLELAARAGYNP
                     EAAVSLWKKMMAASKDAPPEFLSTHPSSENRIKDLQALTPKVMPLYLAAKNK"
     gene            8245..9072
                     /gene="dapD"
                     /locus_tag="VNZ84_01950"
     CDS             8245..9072
                     /gene="dapD"
                     /locus_tag="VNZ84_01950"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HWU65742.1"
                     /translation="MDPRQSIIEAAFEDRANINPANAPADIKATVASVLDDLDAGKLR
                     VASRIGDTQQWETHQWIKKAVLLSFRLKDNYLMDDGVTRYFDKVDPKFADYTEEDFKA
                     GGFRVVPNAIVRKGSFIAKNAVLMPSYVNIGAYVGEGTMVDTWATVGSCAQIGKNVHL
                     SGGVGIGGVLEPVQAGPTIIGDNCFIGARSEVVEGVVVEDNCVISMGVYIGQSTKIYD
                     RETGEIHFGRVPAGSVVVSGNLPSSDGKYSLYCAVIVKKVDAKTLGKVGINELLRGV"
BASE COUNT         2417 a         2492 c         2268 g         2260 t
ORIGIN      
        1 cgatcaagct ttttccggaa cccagtgatc cggacaacgt ccgttacaag gtcattttcc
       61 tcaacaatgt attcacgttt gccggtcttg tctcgctgac catgagcttc ttccgctgga
      121 acagccagcc gctccttggc aaaatagact tgttatttgg ttgcctggct ttcgcattac
      181 tctgggggct acaccgttat aaaaaaaaca tagaactggt ttccagtatt gccttgtggt
      241 tatgctttgt tcagttcctg actgtgtttt ttatcgctac cgatagtgct tcccgcagtg
      301 ccttgttgct catcatcctg gcggctgcgt tttacctgaa aggccgcgtc acaggttatt
      361 acatgttagc catcttgctg atattggttg tgggcaacca catgattcaa atattccctt
      421 ccgattatca tcacctcgac atcctgagcc tgtgttttta tcttttagcc cagtttttta
      481 ttatccgtaa ttatgagtgt ttacgtgaat cgcaaaccag ccacttgaag gcgctgaatg
      541 tcaacctgga agccctggta aaacaacgca cagaccaatt ggctgcagcc aataccgcat
      601 tggaattcga aaaagagaac ctgaagcgcc tctcaagcac ggaccattta accggcctga
      661 gcaaccgtca tcacttcgaa accgtgtttg cggaacacac gcccgtctct cacaaaaaga
      721 tgactgacgc cctcattctg atagacatcg accactttaa aaagatcaac gatacctatg
      781 gccatgtgtt gggagacaca gtgattaaag ccgtcgccgg ctgcatcaaa aaacatagcc
      841 ggatttctga tatcaccgta cgctggggcg gcgatgagct gattatttac gccccacgta
      901 caacacttaa acaggcaaca cggttggcag aaaaggtacg ccatcaaatt catgccatgc
      961 accttgccgg tataggctcc attagcatca gtgcaggggt tgccgtatta catcctggag
     1021 acaccctgtc acaattattg cagcgggctg accatgcatt gtatgcagcc aagcaggcag
     1081 gacgtaatag tgtacatgca gcagcgctgg actaagcctt aacaattagg tacctttgcc
     1141 acatcctgtt ttttgtagcg cccatgatac ttagaaaagc tgttgcagtc cttaccttcg
     1201 cttgcctgca actattatgc agccctgcct tgcatgccga gcaatggcaa acagtaccag
     1261 gccgcgaaaa attcctgacg gtttctgtcg acctcgactc attgcaagtc ggccagcatg
     1321 aagacatgat tttcttcaag ctcagggcag acgatcacga cgacaccatt gccacacgtt
     1381 ttgtacaagc ctcgtgtagt gaattttcga tggaggtaga gcgcgaatcg gtgttccata
     1441 aatcgaccca gacatcagaa gccctggact ggaaaggcaa cccggcgctc acgggccacc
     1501 ctgaatttgc cgaggccagg ctatctttct ggggcgtcac acaggcatac gggcaagcca
     1561 tttccctggc atgccgccgg acgggtaacc gggcacctga gcagttacaa agcctgttac
     1621 cggaaaactg tgcagtgaat catccattac gcaaagtggc gtgtgctaac cacgccagtt
     1681 ggcgagcaaa ttacaaacta tacttttcac gctcgcatga cgtgattcat aactgcggga
     1741 tttccgaaga gcgcatgacc aaacaagcag cctggctcat taaaagcgtc atgcgctgca
     1801 aagatgaggg ctgtggtaat gaaatcctgg aaaactggat acacaaacgt gggcaggata
     1861 tcgccaacgt gattcatatg cagaaaaata cgtctaccgc ctggaatgca tcaacccagc
     1921 cagcgccatt atgtgaatca ctcaatcagg ctgaccaggc gattgccagc ctgcagactg
     1981 aagagattta tgccaaagcc aaatacctgg gatgcctgaa acgtgaaacc gcaagattac
     2041 gcgcaaccaa cacccccgcc gggacactgg ctcaacaggc agaacaatta tgcgaaggac
     2101 catttcagga gtggcatgat gccatctcca aacaatccgc aatgcgcgac taaaccatag
     2161 ggcttgtcac ccatagtgca tcgcattagc tgccacagcg tcgtgcacga aacatgaaga
     2221 cagcatacag gccaaacagg ataaaactga tcaagccaag ttgcggcaat gtcagcccaa
     2281 gaaatgtcca gtctatggcg gcacaatccc ccgtgccacg gaataccagg gttagcgctt
     2341 ttttaagcgg gaagttttca aacatatagt caaaaccaac gccacaatcg gcaatgatct
     2401 cttctttatg tgcttgtagc caccaatggc gaatggctac acccgcgccg cccagtgcgg
     2461 tcagtacctc gagtaaagcc agcccggcat gtaaccagga ttgaggagaa acaaaagcat
     2521 gcaccaggaa caacgctccc aggctcataa aaacaatacg ctgcgagata cagagcggac
     2581 agggttccag ctggttttgt acttgtatga ataaagccag tgcgacaata ccgaacccag
     2641 ccaggaatcc aagcacatag ccgccacgac cgttaaacca ctgacacatt gcatcctcct
     2701 gcacgttgat gaagctcaaa ttgtaataga taacgtgctt atgatgcggc ctgattcaat
     2761 aacgttccat gatttagctg cgctatgaac tcgcaggcgt gccgttgggc tgttgcatgg
     2821 cagacttttg tctgacttcg tgatgttttt tcttgtaata ctgcgcaatc cgcttcacat
     2881 agtcacgcgt ctcaggataa ggcgggattc cccggtatct gagtaccgcg ttttcgcccg
     2941 cgttataggc agctgcaatc aactcaacat cccctttgaa atagccaatc aaccagcgca
     3001 gataagacag gccaccttta atattctgct caggatcata cgcatctttg acatgaaacc
     3061 tggcttgcgt atcaggcatt aactgcatca atccctgtgc ttttttgcga gaagtcgcat
     3121 taggattaaa gccagactcc accgcgatca cggccatggc aaagtccacg tccacaccat
     3181 agtctggtgc atgtttcttt acgagctggt ataccgggcc acgtttgcgg aagagtatct
     3241 gggcagctga atgagcgtgt ttctgcatac aggccggcag ttttgccagc tcgggattgc
     3301 ctggctcttt atccagccac tccatggcat cacggtgacc ctgtatcgca gccaggctaa
     3361 aaaagcgtgc cgcaacatca tgattgacct ccaccccttt acccatgctg taaaaccaac
     3421 ccatggcaaa ttgtgcatcg gcatcacgcg actcgccagc aagggcacaa taatgggcat
     3481 gggcctgctc ataactgtta agccccagtg cgttcttggt acccgttgcc aggctcacct
     3541 ggagtttgat cagttgctta tatgcgtcat tcctaatcac tgattgctca cggctgtagc
     3601 tggaaagcgg ctcacttggc gcaagaacgg gtttatcttc cccggcggat actgcctgca
     3661 cctcttccgc caatgcgcgc tcggtcactt catcgtctgc ataagacaaa gctggcgagc
     3721 atacgcaaga tagcgcaagc aacaagaaac tactacgcgc aagggatatg tgtattttca
     3781 atgacatgta gccaccataa aagtatgaag atgaatagcg ccaaggcact tgactcatat
     3841 tgaatgaccc ataaacgcac ccaaagtgcc cttaacggcc atccaaagtt caagtgcgca
     3901 aaaaccatcc agcgtacaag ttatttttta tattggtctt tagtggtcat gctcagcaaa
     3961 agcatagcca aacatccaca ggctgatgga taatgtgtag ctatggctaa cctgttatcg
     4021 acctcctcca aacaaatctt gagtgtcagc gacctgacgc gattgactaa agagctgctg
     4081 gaaaccagct ttccgctgtt ctggatcaat ggtgaaatct ccaatttcac gcgtgcagcc
     4141 agtggtcact ggtatttctc actgaaagat gacagggccc aggtacgctg cgtgatgttt
     4201 aagggccgta acagcctgat agaaaccatg ccacgtgaag gtgacctgat cgaagcgcgt
     4261 gccactgtga cactgtatga agcgcgcggt gactttcaac tgaccataga gtttttgcag
     4321 ccggcgggtt tgggcaggtt atacgaggct tttgagcaac tcaaacagcg cctgcaagcg
     4381 caagggctat tcgatgcatc ccgaaaaaaa gcgataccgg ccaatccaca acgcataggc
     4441 gtggtgactt cacccgacgc ggcagcgtta cgcgatgtat tgacggccat acgccgccgc
     4501 taccccggca tgggagtcat tatttacccc acgcccgtgc aaggcaaagg ttcggccgaa
     4561 ctgattgcac aggcaataca attggccgac cagcggcaag aagtcgatac actgctgatt
     4621 tgccgcggtg gaggcagtat tgaagatttg tggtctttta atgaagaaat tgtcgcacaa
     4681 gcaattgctg cttgctgctt gccaaccatt agcggggtgg gccatgaaac cgactttacc
     4741 attgcagact ttgtggcaga cttacgcgca gccaccccga cagcagccgc agaattagcc
     4801 tgccctgacc aatcacaatt gcgccagcaa gtagaacaat cccggctacg cctgatgcgt
     4861 gccatgcaat ctctgttacg ccagcatgcg caaacactgg attatctgtc gcgccgcctg
     4921 gtgtcgccag cgcagcagtt gcagcagcaa aaacaggtgc ttcagcaatg gcaacatcgc
     4981 ctgcaattat ccatgcagca atacctgcaa tcgcaccgcc gcaaactgga gcactgtgcc
     5041 accagcctgc aacaactgaa tccgcaccag gtgctggcac gcggttacgc catcgtgcag
     5101 caacaaaacg gtcgtgcagt gactgatacc gcccaactga cagtaggcga aagtttgcag
     5161 ctgaccttgc accgcggaca ggccgcagtc gtggtaacac aatcacctat caacaaaaaa
     5221 taaccttccg tgtatggttc tgtgaagctt aagcttgtgg cttaatctcg caaaaaacgg
     5281 gtgataaagt cgaatatttc tgtcaaaagg tgttatttct gtcaaagttt ttggtgaagc
     5341 cttaaaatta accgataata atcgtttgtc ttcagctgtt cacacaatgg accatacaaa
     5401 aaatcaagcg gcgctccata ccatggcgct cgccgcactt ggtgttgttt tcggggatat
     5461 cggcaccagc cccttgtata cgatgaagga agtgtttgct gttggccatc acccgctgtt
     5521 gctgacacaa gacaatgtct atggcatttt gtcactgatc acctgggctt tgctgctaat
     5581 cgtttctttc aaatatgtgg catttatcat gcgtgccgac aaccgtggcg aaggcggtat
     5641 catggccttg ctgtcactgg caagccgtta tgcaggcggt gaccccgaac aacgcaaacg
     5701 catcatgatg ctgggcatta tcggtgcctg catgttttat gccgatggca tgattacgcc
     5761 ggccatttcg gtattgtctg cggtggaagg actggaagtc gccgcacctc aactcgatag
     5821 ctggattgtg ccaattacat tgtgcgtatt gtttgggctg ttctgggcgc aaagcaaggg
     5881 caccgcggtc gtcggcgcat tttttggccc gattatgctg acctggtttg ccgtgctggg
     5941 catacttggc gtgctcaaca tcacccacca cccagaaatc ctcaaagccc tgagcccggt
     6001 ttacgccttc ctgttttttt atacccaacc caaaattgct tttattgcct taggtgcggt
     6061 cgtactggcg gtgacgggcg ctgaggcact ttacgcggac atgggccatt ttggccgcaa
     6121 accaatccgc ctggcctggt ttttgtttgt cctcccagcc ctgatttgca attactttgg
     6181 tcaaggtgca ctgatcctgg cagaaccaga atccatccag aacccgtttt accacctggc
     6241 accagattgg gcattgttcc cactcattat cctggcgacg ctggccaccg taattgcctc
     6301 acaggccgtg attaccggcg ctttttccgt ctcccgccag gccttgcaac tgggtttcat
     6361 cccccgaatg catgtgtcac acacttcaga acaaactgaa ggccaggtat atatgccacg
     6421 cgtcaattgg ggcctgatgg tcgcagtaat ggggctggtc ttgatttttg gcagttcagg
     6481 cgacctcgct gccgcatatg gcattgcagt gacaggtgac atggtcatca cgaccctgct
     6541 ggctgcggtt gtattccatc atttgtgggg atggagcaag ctcaagactg ccacactgat
     6601 tactactttt ctagtgatag acctttcctt ctttgctgcc aatatcctca agattcctga
     6661 tggtggctgg gtaccattgc tgataggcct gattattttt acactcatgc gcacctggaa
     6721 aacgggccgc agactgctct atggcatcct caagtctgag tccatggagt tgattccttt
     6781 tatccaggcc attggtgcac acccccctgc ccgcgtcaac ggcactggcg tgttcatgac
     6841 tcccaacccg gacggtgttc cacatgcttt gctgcataac ctcaagcaca acaaggtgtt
     6901 gcacgaaaac atgattatcc tgacagtgcg ttttgaggac tatccttaca ccagccagga
     6961 agagcgcgta tcggtagaag cattgccgta tggtttttac cgggtaacga tacgttacgg
     7021 ctatatggat gaacccgacc tgccaagaga cctggcgttg acagaaccat ttggcctcag
     7081 catggatacc atggatactt cattctttat cggcaaggaa atcctgattg cttcagacaa
     7141 gcctggcatg tccttctggc gtaaaaagat tttcattggc ctgtttcgca ccgcggaaac
     7201 cattaccaac cagtttaaat tgcctccaaa ccgcgtggtt gaactggggt cgcaggttaa
     7261 aatctaagag aaaaccgtga gccactatgc tgtgcttaaa ctaaccagcc agtcagcaga
     7321 aaggaagact atgttcaata ttaaaaccat gactgtcgca gtgatgtgcg ccctgctgtc
     7381 agcctgtgcc agcacgacca gcaacagtgt atctggcgta caacgcagcc agttcctgtt
     7441 actgccatca gccaccgtcg acaagatgtc ggcacagtcc tatacacaaa ccctgcagga
     7501 tgcacagaag aaaagtacac tgaatgcaga taaagcgctg ttgaatcgag taaccaccat
     7561 ttcgcaccgc ctgattgccc aggtgggtgt attcaggcct gatgccacca aatggaaatg
     7621 ggaagtcaat gttgaaaaaa acgaccagct caatgcctat tgcatgccgg gtggcaagat
     7681 catggtgttt tctggcctgg cagataaatt aaacgctact gacgatgaac tggcagcggt
     7741 gattggtcat gaaatatcgc atgccttgcg tgaacatggt cgcgaacgta tgtcacaggc
     7801 ttatgtacaa caatttggct tgcaggcact cgctgcatat gtcggcggca cagcaggcaa
     7861 tatggccgcc cagggagcaa acatgggtag ccagctgcta ttctctttac ccaatggccg
     7921 agaacaggag cgtgaagccg accgcatggg tcttgaactg gcagcccgcg ccggttataa
     7981 cccggaagct gcagtgagct tgtggaaaaa aatgatggct gccagcaagg atgcgccacc
     8041 tgaattcctg agcactcacc cctccagcga gaaccggatc aaggatctgc aagcactcac
     8101 cccaaaagtc atgccgctct acctagcggc caaaaacaaa taaacgcttg cttacggggc
     8161 tgacagaact ctgcggcccc tgacccatca aaattaggtt aaaatttcgg gtttcactgt
     8221 tatttaaccg aattagagaa catcatggat ccacgtcaaa gtattattga agcggctttt
     8281 gaagaccgcg ccaacatcaa cccggccaat gccccagctg atatcaaagc cacagtggct
     8341 tctgtattgg atgatctgga tgcaggtaaa ctgcgtgtcg cgagccgtat tggtgatact
     8401 caacagtggg aaacacacca gtggatcaag aaagccgtat tgctgtcatt ccgcctcaaa
     8461 gacaactacc tgatggatga tggtgtcacc cgttactttg ataaagtgga tccaaagttt
     8521 gccgattaca ctgaagaaga tttcaaggca ggtggcttcc gcgtggtgcc taacgccatc
     8581 gtgcgcaaag gctccttcat tgcgaaaaat gcagtgttga tgccttctta tgtcaacatc
     8641 ggcgcttacg tcggtgaagg caccatggta gacacctggg caaccgttgg ttcctgtgct
     8701 caaattggta aaaacgtaca cttatccggt ggcgtaggca ttggcggcgt actggagcca
     8761 gtacaagccg gcccaaccat tattggcgat aactgcttta tcggtgcacg ttccgaggtg
     8821 gttgagggcg tggttgtgga agacaactgc gtgatttcca tgggtgtgta tattggccag
     8881 tcgaccaaaa tttatgaccg cgaaaccggc gaaatccact ttggccgcgt tccggctggc
     8941 tcagtcgtgg tatcaggcaa cctgccttcc agcgacggta aatacagctt gtactgcgca
     9001 gtcatcgtga aaaaagtcga tgctaaaaca ttgggtaaag tcggcattaa cgaactatta
     9061 cgcggcgttt agcatttacg cattgataga gaatcacccc agtatcaccc gctcaagtta
     9121 tctgcacatc gggtcgatat aagagtgatt ctcatcatcg gttttattaa gtcatcgcat
     9181 taagtatgga gaattggcag gcaagaaaca aaacgcagta gagtcacacc actaacaatc
     9241 acaatgaccg tgcaatttta tgacctcaac acacatgctt attttgctgc ttttcacatc
     9301 cagcgtcatc ggatgttttt ttgcatttaa gctggttgat cacctgcgta tcgcggctac
     9361 caaagagcac gatgcactgc tcaaaaaata ctcaatcatc actgctggcg cgatctttct
     9421 gctacacgct ggctacc
//
LOCUS       DATMSK010000059         3507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_75323,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000059 DATMSK010000000
VERSION     DATMSK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75323"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VNZ84_01955"
     CDS             <1..314
                     /locus_tag="VNZ84_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65743.1"
                     /translation="FIELCCHHPAAANQTFLISDDHDVSMTELIHTIRTAMGHRSFLL
                     PIPSAILKFALNILGKKNLSERLLNNLQVDTSKAKQLLGWKPVISFEEGIKQTVNEYV
                     A"
     gene            304..879
                     /locus_tag="VNZ84_01960"
     CDS             304..879
                     /locus_tag="VNZ84_01960"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWU65744.1"
                     /translation="MLLKRIWDILLSLIALVLLSPFLLFTYLIVKLSSKGPALYWSQR
                     VGQHNQLFRMPKFRSMQVGTPQIATHVMNQQSNPNSYLTPVGSFIRKTSLDELPQLWS
                     VLKGDMSIVGPRPALFNQDDLIGLRTSKNVHQLKPGITGWAQVNGRDEIPIPQKVSLD
                     AYYLAHQSTWLDLKIIALTAIKVIKRDNITH"
     gene            897..2816
                     /locus_tag="VNZ84_01965"
     CDS             897..2816
                     /locus_tag="VNZ84_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HWU65745.1"
                     /translation="MHKLPIKLLSILAFFHDAVVVVAAWWLAFALRFNFHIPPGFSQF
                     IWQSLPIIFLFHAISFYTFGLYKGVWRFASLPDLKRIMRAIGFAALMSASYALLVHPS
                     IIIPRSVLILNPMLLVLAMGGSRFIYRAWKERRLFNSIALQGKPVIVIGVEDASFNLI
                     KELSKNPEWRVVAMIDPSNKLVGREIMHTKVEGGLEDMAQIAARHSCQHCMIAMPGSR
                     HEKRRLAVEAARKVEGMEVLTLPAMSDLISGRVSLSQIRKIDVEDLLGRDVVQLDTHG
                     LSSMLANKVVLVSGAAGSIGSELCRQILSFQPTQLICLDISEYALYTLQQEFSQWVTD
                     TELVYLVADVKNQTRIQRILQSHQPDVVLHAGAYKHVPMMEHFNVVEALQNNAHGTYQ
                     IAAACQAAGVPTFVLVSTDKAVNPTNVMGASKRLAELICQGLQHGDNSHTRFITVRFG
                     NVLGSSGSVIPKFREQIAHGGPITITHPEITRYFMSIPEAAQLVLQAATMGNGGEVFV
                     LDMGTPVRIIDLARDMIKLSGLQEQEIRIEFTGLRPGEKLYEELLADDENTLPTSHEK
                     LRIAQARSADARWLGNLLEWLSNIPHLQEAQIKQDLQRWVEEYQPDTRPAPDVSLRGG
                     ALSLDTLPSPSQQIH"
     gene            2910..>3507
                     /locus_tag="VNZ84_01970"
     CDS             2910..>3507
                     /locus_tag="VNZ84_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU65746.1"
                     /translation="MPHITLDNASLAFGHHALLAQASFQLDPGERVGLIGRNGAGKSS
                     LLKAIAGTIKLDDGTVWRAPNVRVVYVPQEPELDNAHTVFEAVAEGLGSLQQTIIDYH
                     QVTHDMGMPDANIEALMERMQALQHEMDAQNGWVAQSRVETVLSRLKLDADVLVSTLS
                     GGWRKRVALGRALVAEPEVLLLDEPTNHLDLEAIEWLED"
BASE COUNT          884 a          797 c          892 g          934 t
ORIGIN      
        1 gttttattga gttatgctgc catcatccag cagctgcgaa tcaaacattt ctcatttctg
       61 atgatcatga cgtatctatg actgagctga ttcacacaat taggaccgca atgggtcaca
      121 gatccttttt actaccaatt ccttcagcta ttctaaaatt tgctttgaat attttaggga
      181 aaaagaacct gagcgaaaga ttattgaata accttcaagt ggacacatct aaagccaaac
      241 aattgctagg ctggaaacca gtgattagct ttgaagaagg tattaaacaa acggttaatg
      301 agtatgttgc ttaaaagaat ttgggatatt ttattgagct tgatagcttt ggtgttattg
      361 agcccatttt tgctattcac ttacttaatc gtgaagctca gctcaaaagg cccagcgtta
      421 tactggagtc agcgtgtagg tcaacacaat cagctattca ggatgccgaa attcaggtcc
      481 atgcaagttg gtacgccaca aattgccaca catgtaatga atcagcaaag caatcctaat
      541 agttatctca cgcctgttgg gagttttatc cgcaaaacca gtttggatga gttaccgcaa
      601 ttatggagtg ttttaaaagg agacatgtct attgtgggac cacgccctgc attgtttaat
      661 caagacgact taatcggctt acgcacatcg aaaaatgtac atcaacttaa acctggcatt
      721 actggctggg cacaagtaaa tggccgtgac gagattccaa taccgcagaa agtaagttta
      781 gatgcctatt atctagcgca tcaatcaacg tggctggatt tgaagattat tgcgttaact
      841 gcgatcaaag tgatcaagcg tgataacatc acacactgac tctatgttta acaaacatgc
      901 ataaacttcc tatcaagctt ttatcaattc tggccttttt tcacgacgca gttgtcgttg
      961 tagctgcctg gtggttagcg tttgcattac gttttaactt tcacatccca ccaggattta
     1021 gccaatttat ttggcaatcc ttacccatca tttttctatt ccacgcgatt tcgttctata
     1081 catttggctt atacaaaggc gtgtggcggt ttgccagcct acccgatttg aagcgtatta
     1141 tgcgtgcaat tggtttcgct gcattgatga gtgcatcgta tgcgttgtta gtgcatcctt
     1201 cgatcatcat tccgcgctca gtactcattc taaatccaat gcttttagtg ctagctatgg
     1261 gtggcagccg ctttatttat cgcgcctgga aagaacgtcg cttattcaac agcattgcgt
     1321 tacagggaaa gccagtgatc gtcattggtg tcgaagatgc atcctttaat ctaattaaag
     1381 agctgagcaa aaaccctgag tggcgcgtgg tggcgatgat agatccgagc aataagctgg
     1441 ttggccgtga gatcatgcac accaaggtcg aaggtggctt ggaggatatg gcgcagattg
     1501 cagcacgcca cagttgccag cattgcatga ttgcgatgcc ggggagtcgt catgagaagc
     1561 gtcgattggc agttgaagcc gcacgtaaag tagaaggcat ggaggtgctg actttacctg
     1621 ctatgtcaga cctgatcagt ggccgtgtca gcttgtccca gatccgcaag attgatgtag
     1681 aagacttgct ggggcgtgat gtggttcagc tggatactca tggcttgagc agtatgctgg
     1741 cgaataaagt ggtgctggtc tcgggtgctg ctggctcgat tggttctgag ctttgtcgcc
     1801 agatattgag ctttcaaccg acgcagttga tttgcctgga tatttctgag tatgcgcttt
     1861 ataccttgca gcaggaattt agccagtggg tgacggatac cgagttggtg tatctggtgg
     1921 cagatgtgaa aaaccagacg cgcattcagc gtattttgca aagccatcaa ccagacgtag
     1981 tgttgcatgc tggcgcttac aagcatgtgc cgatgatgga gcattttaat gtggtggagg
     2041 ccttgcagaa caacgcgcat ggcacctacc agattgccgc ggcttgtcag gcggctggcg
     2101 tgccgacctt tgtcttggtt tcaaccgaca aagcggtgaa tccgaccaat gtgatgggtg
     2161 ccagcaaacg gctggccgag ctcatttgcc agggattgca gcatggcgac aatagccaca
     2221 cgcgttttat cacggtgcgt tttggcaatg tgctgggcag cagtggcagc gtgattccca
     2281 agttccgcga acagattgcc catggcggtc cgattactat tacccacccc gaaattacac
     2341 gctactttat gtctatcccc gaggcggcac aattggtgtt gcaggctgcg accatgggca
     2401 acggtggcga ggtgtttgtg ctggacatgg gcacacccgt gaggattatt gacctggcgc
     2461 gcgacatgat taaattgtct ggcttgcaag agcaggaaat ccgtattgag tttaccggat
     2521 tgcgaccagg cgaaaaactg tatgaagagc tgctggcaga tgatgaaaac acgctgccga
     2581 ccagccatga gaaactgcgc attgcacagg cacgtagtgc agatgcgcgc tggctaggca
     2641 atctgctgga atggttaagc aatattccac atttgcaaga agcacaaatc aagcaagacc
     2701 tgcagcgctg ggtggaggag tatcaacccg atacccgccc ggcacccgat gtttcgctgc
     2761 gcggaggtgc gttgtcgcta gataccctgc cctcgccttc acaacagata cactgataga
     2821 ttcttttatt tacgcattaa gcttattgta tggtttgatg tcgtttacaa tatcgaatcg
     2881 attcaacaac ctctgcccca tcccccttta tgcctcatat tacgcttgat aatgctagcc
     2941 tagcatttgg tcatcatgca ttacttgccc aagcctcatt ccagttagac ccaggcgagc
     3001 gcgtcggttt aattggccgc aatggtgccg gtaaatccag tttactgaaa gccattgctg
     3061 gtaccatcaa actggatgac ggcactgtat ggcgcgcacc gaacgtacgc gtggtctatg
     3121 tgccacaaga accagaactg gataatgccc acaccgtatt tgaagccgtg gcagaaggtt
     3181 tgggcagctt gcagcaaacc atcatcgact accatcaggt cacccatgac atgggcatgc
     3241 cggatgccaa tattgaggcg ctgatggagc gcatgcaggc actgcaacat gaaatggatg
     3301 cgcaaaatgg atgggtagcg caatcacgtg tcgaaaccgt gcttagccgc ttgaagctgg
     3361 atgcggatgt attggtctct acactttctg gtggctggcg taaacgcgtt gcgctcgggc
     3421 gggcgctggt ggcagagcct gaagttttat tactggatga gccgactaac caccttgacc
     3481 tggaagccat cgagtggctc gaagatt
//
LOCUS       DATMSK010000060         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_146153,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000060 DATMSK010000000
VERSION     DATMSK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146153"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VNZ84_01975"
     CDS             complement(<1..272)
                     /locus_tag="VNZ84_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU65747.1"
                     /translation="MLDTSPVRTKVGRPKSGTEQERHAQLLEQALELFMTEGFTRTSI
                     ARIASTCGVSTRTIYERFGNKDELLVAALKHMVEQDVLAMARVDGM"
     gene            455..994
                     /locus_tag="VNZ84_01980"
     CDS             455..994
                     /locus_tag="VNZ84_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HWU65748.1"
                     /translation="MALKSTIYKIDVNIADMDRHYYQQHHLTLAKHPSETDERLMVRL
                     LAFVLYADEALLFGKGLSEDDEPDLWQKDLTGAIERWIGVGLPDEREIRKACGRAAEV
                     VQVLYGGRTAEMWWQQNQKAVLKLENVTVINLPETEQLANAASRGLAISFTIQDRQVL
                     VSYEGGSFEILPEWLKEPE"
     gene            complement(1076..1591)
                     /locus_tag="VNZ84_01985"
     CDS             complement(1076..1591)
                     /locus_tag="VNZ84_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65749.1"
                     /translation="MRRRLYFVLPNIKSAQAMMNELLLDRIEEGHIHFHAKNEQSLGS
                     LPKANSVEKSDVVYSALAGFVFGAVFGLLGGLLAYLVPWWFGEVTLMVIPYCMILGAL
                     SCAAWAAAVATAAPSYRIKAYKSQIEQGSILMIVSVPLRRLGDIRQRLMQQHPEAMYS
                     GVWPAEHRLFP"
     gene            complement(1728..2828)
                     /locus_tag="VNZ84_01990"
     CDS             complement(1728..2828)
                     /locus_tag="VNZ84_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65750.1"
                     /translation="MNAEGQVIARSDYEDKARYLAFVKENHVPCVSLHWLALLHPLVP
                     HYSGEEGELRYREIEYQRMPLMAYLSFDDVSQLSRGDLIRLGLVCQPWHSETLPFTEQ
                     FLQAFEKNNCYDRYWDEARQDPWSTTRIMCTGQNFVVVGSHQHRVFTNERTGIKRHYQ
                     NQYFLLFLIAHFQKAALLMLSDRMVQAISRLNIQSEDSVKVFRSRIRQATAVFLRFTH
                     RYWFENVSDQAVAKDLFSLMLKQLDSVSLFEKTRRRIMDMAEYLEGEEIKRQADTVVR
                     LTVVTILGLIGTMTSGLLGMNLIDLTQSTLLEKLAYFSIVFIPVSLLTFYTVIKSRRL
                     SLFLDALSNEGARMGYKLAKLRSVWMGKLDEH"
BASE COUNT          795 a          791 c          679 g          711 t
ORIGIN      
        1 ctcatgccat ctacccttgc catcgccaac acatcctgct caaccatatg ctttaaagca
       61 gcgactagta gttcatcctt gttgccaaat cgttcgtaga tggtccgggt agaaacgccg
      121 caggtgctcg caatacgtgc aatactggta cgcgtaaaac cttccgtcat gaacaattca
      181 agcgcctgct caagcaattg tgcatgacgc tcttgctcag tccctgactt gggtcgacct
      241 accttggttc tcactggact tgtatccaac atatggcgtg tctcaataat gatgacatta
      301 aataaaaatg aaacgtttca tttttatttt acccatttat gtaaaagtag caagcgtttc
      361 gttgtaaccg tgcagataca ttcaagtcta tcccatcttc aaccatgggt ttgctatact
      421 cgctgcactc ttaactgacg cagaaacagc ccgcatggcc ttaaaatcca caatctataa
      481 aatcgacgtc aatattgccg acatggaccg gcattattac cagcaacatc acctcaccct
      541 ggcaaagcat ccttcggaaa ccgatgagcg cctgatggta cgtttattgg cttttgtact
      601 atatgccgac gaagcattgc tatttggtaa aggcttgagt gaagacgatg aaccagacct
      661 ctggcaaaag gatttgacgg gcgcgattga acgctggatt ggtgtaggtt tacccgatga
      721 acgcgagata cgcaaagcct gtggccgggc agcagaggtg gtacaagttc tctacggtgg
      781 cagaaccgcc gaaatgtggt ggcagcaaaa ccagaaagca gtactcaagc ttgagaatgt
      841 caccgtcatt aacttgcctg aaacagaaca gctcgccaat gctgcaagcc gcgggctagc
      901 catcagtttt accattcaag atcggcaggt attggtcagt tatgagggtg gcagttttga
      961 aatcttaccg gaatggctta aagaacctga gtgacaggtc acaaacaaag tgcccatcaa
     1021 tataaaaaag ccagcattgc tggctttttt atcaatgacc gcgcttaaca ccagctcagg
     1081 gaaaaagtcg gtgttctgcc ggccaaacgc cgctatacat cgcttccgga tgctgctgca
     1141 tgagccgttg ccggatatcc cccagcctgc gcaatggcac cgacacaatc atcaaaatac
     1201 tgccttgctc aatctgtgac ttgtatgcct tgatgcggta actaggcgcg gctgttgcga
     1261 cagccgctgc ccacgccgca caagagagtg cgcccaggat catgcaataa gggatgacca
     1321 ttaaggtgac ttcaccaaac caccatggca ccagataagc cagtaaaccg ccaagcaaac
     1381 caaacacagc cccgaacaca aaaccggcca aggcgctata aaccacatct gatttttcta
     1441 ccgaattcgc cttgggaagg ctacctaaag attgctcatt tttggcatgg aaatgaatat
     1501 gtccctcttc aatcctatcc agtagcaact cattcatcat cgcttgtgca cttttaatat
     1561 taggtaaaac aaaatagagc ctgcgtctca tgtcgttctc ctatcaagat tgccattatt
     1621 agcttgtcaa aattggatag gtgaccgata caaagattca ttttttaaca actgtctggg
     1681 aaaacctggc gcggatactt actgattacc gcgtgccgtc cacactctca atgctcatcc
     1741 agtttcccca tccagacact ccgtaacttg gcgagtttat agcccatccg cgccccttcg
     1801 ttggacagtg cgtccaggaa cagggaaagc cttctggatt tgatcacggt ataaaaggtc
     1861 agcaagctga ccggaataaa gacaatggaa aagtaagcca gcttttcgag caaggtggat
     1921 tgtgtcagat cgatcaagtt catgccaagc aggccgctgg tcatggtgcc aatcaatccc
     1981 agaatcgtca ccactgtcag tcgtacaacc gtatccgcct ggcgtttgat ttcctcacct
     2041 tcgaggtatt cagccatatc catgatacgg cgccgggtct tctcaaacag gctcacggaa
     2101 tccagttgct tcagcatcag tgaaaacagg tctttggcca ccgcttgatc cgacacattt
     2161 tcaaaccagt agcggtgcgt aaagcgtaaa aacacggccg tcgcctgacg aatacggcta
     2221 cggaatactt ttaccgaatc ctcactctgg atattgaggc ggctaatggc ttgcaccatg
     2281 cggtcagaca acatcagcaa cgctgccttc tggaaatgtg ctatcaagaa cagtaaaaag
     2341 tactggttct ggtaatgccg cttgatgcct gtgcgctcat tggtaaacac gcgatgctgg
     2401 tgactgccga ccaccacgaa attttgcccc gtacacatga tgcgcgtcgt actccacggg
     2461 tcctggcgcg cctcgtccca ataacggtca taacaattat tcttttcgaa tgcctgcagg
     2521 aactgttcgg tgaacggcaa cgtttccgaa tgccagggct ggcagaccaa gcccaggcga
     2581 atcaggtcac cgcggctgag ctggcttaca tcgtcaaacg acagatatgc catgagtggc
     2641 atccgttgat actcgatctc gcggtagcgc aactcaccct cttcgccgga gtaatggggg
     2701 actaatggat gcaggagtgc cagccaatgt aatgaaacac aaggcacatg attttctttg
     2761 acaaaggcca ggtaacgcgc cttgtcttcg taatcagacc gcgcaatcac ctgcccctct
     2821 gcattcaacc attcaaccag ttgcgcacag tgcccggctt cacccgtatc atctcagtag
     2881 ttagggtaag tacgccccaa acggtacatg acctccatgg cggactttaa gggcagatct
     2941 ttgccagtga cttcaaatgc caggatggca gatcgg
//
LOCUS       DATMSK010000061         5809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_75688,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000061 DATMSK010000000
VERSION     DATMSK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75688"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1164)
                     /locus_tag="VNZ84_01995"
     CDS             complement(4..1164)
                     /locus_tag="VNZ84_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU65751.1"
                     /translation="MDISQLSFVWYRGLQEISPAAWQTLAGTHPMLDYRFLRAFELSA
                     AVDHQTGWIPYHLTVFDGEQLLAAVPCYLKTHSYGEYVFDWSWADAYEQAGGQYYPKL
                     ISAIPFSPVTGPRLLIHPDYAEPQALAASMLAQMQAFCQAHSLSGVHILFPDELSAKE
                     SADAGWLRRNGVQFRWENAGYADWEAFLATLNRDKRKKIRQERQKVSQQGVDCREVNG
                     FEVSKTELDLFYRCYCHTYYRHGSSPYLPLAFFEQVSREMPEQFRLFIAAQNGEDVAA
                     SLCVLGADTLYGRYWGSLRDISCLHFELCYYQPQLFCIREGIRYFEGGAQGVHKLARG
                     FAPYATCSYHYLSHADFQQSVARFLAREAGTMQHYVDELEERSPYKATPAAE"
     gene            complement(1252..1818)
                     /locus_tag="VNZ84_02000"
     CDS             complement(1252..1818)
                     /locus_tag="VNZ84_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HWU65752.1"
                     /translation="MLLALPAFADLKLPIRLTDNRIVDNDDVGLSKTLEHNFSPEDRA
                     RLRKALSDYARNADPDHQLMVQKRKAMHDSISQRFNECNRDNDDSLDREEATLCLPQV
                     ARHFSYVDLDGNGVITLDELESAYAQMVERQRAAEAKSEAQQNIVSDSDTDAKLRNKP
                     KLPSAVIPANSSASSTSKDVSATRKRPS"
     gene            2291..3016
                     /gene="ompR"
                     /locus_tag="VNZ84_02005"
     CDS             2291..3016
                     /gene="ompR"
                     /locus_tag="VNZ84_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HWU65753.1"
                     /translation="MAEQLKKILMVDDDLRMRELLQRYLTEQGFNIKSVSDGKEMDKA
                     MTAEGFDLLILDLMLPGEDGLAICRRLRGANHMTPIIMLTARGDEVDRIVGLEMGADD
                     YLPKPFNPRELLARINAVLRRHEIAPKQTTEEKTSAFQFGEFVFDTNTRSLTKNGVSI
                     TITSGEFTLLKVFTEHPRQPLSRDRLMQLARGRELDVFDRSIDVQVSRLRRIIEPDPA
                     HPRYLQTMWGFGYVFIPDGEAPQ"
     gene            3080..4357
                     /locus_tag="VNZ84_02010"
     CDS             3080..4357
                     /locus_tag="VNZ84_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU65754.1"
                     /translation="MLLIAILLAVSIYASLKIFDYFDREPRATAAALQAVTIVNYTRA
                     SLIASHENRRLALLSELSGREGVRVYAADFLEDIEPLPSDPFINLVAQKIRERLGEET
                     IITVNHYDIPGLWISFNVGQDDFWVVIPKIQVDRPFPWHWLGWAAVVGLLSLIGGYLL
                     TARINRPLTMLVHAAERLRNGEPAPKLPEDSVEELREVTHTFNEMAESLARLDAERTL
                     LLAGVSHDIRTPLARLRLAVEMLPPDADYMKHGMIEDISDMDNIIHQFLDFVRGVEGE
                     PTRMMDINDLLHALAERQRRAGRELKISLGPKYFIPIRPLAMQRLLDNLVGNAYNYGK
                     GKVEVRSQVMADKIVISVLDNGPGIPPEHAERLLRPFERMDSARNKYEGGSGLGLAIC
                     NRIAALHGGQLALLNRPEGGLEARLSLPILPNT"
     gene            complement(4362..5753)
                     /gene="cysG"
                     /locus_tag="VNZ84_02015"
     CDS             complement(4362..5753)
                     /gene="cysG"
                     /locus_tag="VNZ84_02015"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HWU65755.1"
                     /translation="MQALPIFFNIAQRLCIVIGGGDVATRKVLMLRKAQGKVTVISPE
                     LCIELKDMYEQGEIEYMPAEFHAEQLTSACLVIAATDDEAVNEAVSVAAKRLNIPVNV
                     VDAPALCTFTMGSIIDRSPVVIAVSSEGNAPVLARHIRSKIETMLPAAYGRIAAIAGE
                     FREQVKARFTNMPARRRFWEEVLNGPLVERVLSGQEQAARELLGELLSQREDAPVRGE
                     VYLVGGGPGDPDLLTFRALRLMQQCDVCVYDKLVSKEVMELVRRDAELIYVGKSRDQH
                     TLPQEEINQLLAKLALEGKRVLRLKGGDPFIFGRGGEEIETLMQHGVPFQVVPGITAA
                     NGVSSYAGIPLTHRDYAQACLFITGHLKDGTIDLDWQAMARPKQTVVIYMGLVGLEQI
                     CAKLIAQGVSPEMPAAVIQQGTTQKQRVVESTLQHLAADVAAAGLKPPSLTIIGEVVK
                     LRSRLNWFTPAEE"
BASE COUNT         1457 a         1623 c         1385 g         1344 t
ORIGIN      
        1 cttttattca gcagctggag tagctttgta aggactacgc tcttcaagtt catccacata
       61 gtgttgcata gtacctgcct ccctagccag gaagcgggcg acactctgct ggaaatcagc
      121 atggcttaaa taatggtaag agcaggtggc gtaaggcgca aaaccacggg ccagtttgtg
      181 cactccctgt gccccgcctt caaaataacg gatgccctcg cggatacaaa acaactgtgg
      241 ctggtagtaa cacagctcaa aatgcaggca ggaaatgtcg cgcaagctcc cccagtaacg
      301 gccatatagc gtatcagcgc ccaatacaca caagctcgcc gctacgtctt caccattttg
      361 tgccgcaata aacagcctga attgctcagg catttcgcgg ctgacttgct caaaaaaggc
      421 cagtggcaaa taagggctgc tgccatgccg ataataagta tgacagtagc accgataaaa
      481 caggtccagc tcggttttac ttacttcaaa accattgacc tcacggcaat ccacaccctg
      541 ctgactgacc ttttgacgct cctgtcgaat ttttttgcgt ttgtcacgat tgagggttgc
      601 gagaaatgcc tcccaatcgg catatccagc attttcccaa cggaattgca ccccattccg
      661 ccttaaccag ccggcgtccg cgctctcttt tgcggataac tcatctggga acagtatatg
      721 cacccctgac aggctatgtg cttggcaaaa agcctgcatt tgcgccaaca tcgaggctgc
      781 cagcgcttgt ggctcagcat aatcagggtg tatcagcaac ctcggtcccg tcaccggaga
      841 aaacggaata gcagaaatca gcttgggata atattgacca cccgcttgct cataagcatc
      901 cgcccaagac cagtcaaata cgtattcacc atacgaatgc gttttgagat agcaaggcac
      961 ggccgccaaa agttgctcgc catcaaacac cgtcaaatgg tagggaatcc agcctgtttg
     1021 atgatctacc gcagccgaca attcgaaagc acgcaaaaat cggtagtcca gcatcggatg
     1081 tgtgccagcc agggtctgcc aggccgcggg tgaaatttcc tgtaagccac gataccagac
     1141 aaaagataac tgactgatat ccatgggaga ttagccgatt aaaatcaggg gatagtgatt
     1201 tagtaaacac gcctgcggaa aaatgggcat gcagggagac atcaaacttg cttaactcgg
     1261 acgtttacgt gtggccgaga catccttgct ggtagagctc gcagaactgt tggcaggaat
     1321 cactgcagaa ggcagtttgg gcttatttct caacttggca tcagtatcag agtcactgac
     1381 gatattttgc tgcgcctcgc tctttgcttc tgccgcgcgc tgcctttcaa ccatctgggc
     1441 ataagcgctt tcaagctcgt ccagcgtaat cacgccattc ccgtcaaggt ctacataaga
     1501 aaaatggcgg gcaacctgcg gtaaacagag tgtagcttct tcacggtcaa gtgaatcatc
     1561 attatcacga ttgcactcat tgaaacgctg agaaatgctg tcatgcatcg ccttgcgctt
     1621 ttgcaccatg agttggtggt caggatcggc gttgcgggcg taatcagaca acgccttgcg
     1681 caagcgggca cggtcttcag gactgaagtt atgttccagc gttttggaga gaccgacatc
     1741 atcattatcg acaatacggt tgtccgtcag ccggataggt aatttgaggt cggcgaatgc
     1801 aggcaatgca agcaacatga ccgttgttag caaaaaaacc tcatttaaat gtttgatcgc
     1861 tctaaacatc cacatccaaa cgtaatacct tatccctgtt tcaccagaag cacttttaac
     1921 atctgccttt tacagtgaaa tcaagcatga ttaacatggc ccagatatcg cctgacttac
     1981 atcaggctgc ctaccataac gcaactatgg ccgcagaatc cagttattta acacaaaata
     2041 gcatctttta caaaatgaat tctatgattg tgagcgggac atcttgcatc atgattgtaa
     2101 atgcaatatt tcattgccac cgctatttgt aactgagttg taaccagcgc ctcttcatgc
     2161 cagatacagc ttggagtata attatgaaga taagtttacg gcctggccgt ttcccatcca
     2221 atgtcaggtc atcctgatag caatcaagcg cgttagcaaa cacgcccgct tctgtgtaaa
     2281 aaggtacgta atggcagaac agttaaaaaa aatcttgatg gtcgatgatg acttgcggat
     2341 gcgcgagctt ttacagcgtt acctgactga gcaggggttt aatatcaaat cggtttctga
     2401 tggtaaggaa atggataaag ccatgacggc cgagggcttt gatttactga ttctggacct
     2461 catgttaccc ggggaagatg gactggccat ttgccgccgg ctacgtggtg ccaaccatat
     2521 gacgccgatt atcatgctga cggcgcgtgg cgacgaagtt gatcgtatcg ttggcctgga
     2581 aatgggggcg gacgattact tgccaaaacc gtttaatccc agggagctgc tggcccgcat
     2641 taacgccgtc ttacgccgtc acgaaatcgc ccccaagcaa acgacagaag aaaaaacctc
     2701 tgccttccag tttggggagt ttgtctttga caccaatacc cgcagcctga ccaaaaacgg
     2761 tgtatccatc acgattacca gcggtgaatt tacgttgcta aaagtgttta ccgagcatcc
     2821 gcgccaaccg ctttcacgtg accgcttgat gcaactggcc cgtggccgcg aactggatgt
     2881 atttgatcgc agtatagacg tacaggtatc gcgtttacgc cgcattatcg agccggaccc
     2941 agcgcatcca cgctacctgc aaaccatgtg gggcttcggt tatgtgttta tccctgatgg
     3001 cgaagcacca caataaactg gctgcattga ccctctaaat cacgtgcgcc tactgcctcg
     3061 cagactactg tcccgcatca tgttgctcat cgccatttta ctggcggtca gcatctatgc
     3121 ctcactcaaa atctttgatt attttgaccg tgaaccacgg gcgacagccg ccgccttaca
     3181 ggcggtgacc atcgtcaact acacgcgcgc ctcactcatc gcttcgcatg aaaaccgccg
     3241 cctggcgcta ttatcagaac tatctggccg cgaaggtgtg cgcgtctatg ccgctgactt
     3301 cctggaagac atagagccct tgccctcaga tccgtttatt aatctggtcg cgcagaaaat
     3361 ccgcgaacgc ttgggcgaag aaacgattat tacagtcaat cactacgaca tccctggcct
     3421 atggatcagc tttaatgtcg gccaggacga cttctgggtc gtcattccta aaatccaggt
     3481 agaccgccct ttcccttggc attggctggg ttgggcggct gtcgtcggcc tgctttcgct
     3541 gatagggggc tacctgttga cagcgcgcat caatcgtccg ctgaccatgc tggtccatgc
     3601 cgccgagcgt ttacgcaacg gcgagcctgc acccaagcta ccggaagaca gtgtggaaga
     3661 gttgcgcgaa gtgacacaca cttttaacga aatggccgaa tcactggcac ggctggatgc
     3721 cgaacgcacc ctattactgg ccggcgtttc ccatgatatc cgcacgccgt tggcacgctt
     3781 acggctggcc gtcgaaatgt tgccaccgga cgccgactac atgaagcacg gcatgattga
     3841 agatatctcg gatatggaca atatcatcca ccagttcctg gatttcgtgc gtggcgtaga
     3901 aggcgaacct acacgcatga tggacattaa tgacttgctc cacgcactgg ccgagcgtca
     3961 acgccgcgcc ggtcgcgagc tgaaaatctc actgggccca aaatacttca tccccattcg
     4021 gccactggcc atgcagcgcc tgctggacaa tctggttggt aatgcctaca actatggcaa
     4081 aggcaaggta gaagtgcgca gccaggtcat ggccgacaag attgtcatca gtgtgctcga
     4141 caatggtcca ggcattccgc ctgaacacgc agaacgcttg ctgcgcccgt ttgagcgcat
     4201 ggacagcgca cgcaacaaat atgaaggggg cagtggcctg ggcttagcca tttgcaaccg
     4261 gattgctgcc ttgcatggcg gccaactcgc attgctgaac cgcccggaag gtggcttgga
     4321 agccagactc tcgctgccta tcttgccaaa tacttagttg ctcactcttc agcaggagta
     4381 aaccagttca ggcgtgaacg caatttcacc acctcaccaa taatcgtcaa gcttggcggt
     4441 ttcaatcctg cagcggccac atcggctgcc aggtgttgca aagtagactc tactacgcgc
     4501 tgcttctgcg tcgtgccctg ttgaatcacg gcggcaggca tctcaggcga aacaccctgc
     4561 gcaatcaatt tggcacaaat ctgctccagc cccaccaggc ccatataaat gaccacggtt
     4621 tgcttgggcc tcgccatcgc ctgccagtcc agatcgatcg taccgtcttt gaggtgacca
     4681 gtaataaaca aacaggcttg tgcataatct ctatgcgtca gcggaatccc ggcatagctg
     4741 gagacaccat tggccgccgt gattccaggc accacctgaa acggcacacc atgctgcatc
     4801 agggtttcaa tctcttcacc accacgtccg aaaataaacg gatcgccgcc tttgaggcgc
     4861 aacacccgct taccctccaa tgccagcttg gccagtaact ggttaatctc ctcttgcggc
     4921 aaagtatgct ggtctcgtga tttacccacg taaatcagct cggcatcgcg cctgaccaac
     4981 tccatgactt ctttactgac caacttatcg tagacacaga catcacattg ctgcatcagg
     5041 cgtaacgcgc gaaatgtcag taagtctggg tcgcctggac cgccaccgac caaatacact
     5101 tcaccgcgca cgggcgcatc ctcacgctga cttaataact cacccagtaa ctctcgtgct
     5161 gcctgctcct gcccagacaa tacgcgctcc accaaaggcc cgttcaacac ctcctcccaa
     5221 aagcggcgac gggcgggcat attggtaaac ctggctttca cctgttcacg aaactcacct
     5281 gcaatcgccg caatgcggcc ataggcagca ggtaacatgg tttcgatttt gctgcggata
     5341 tgccgcgcca ataccggcgc attaccttca ctggagactg cgatcaccac cgggcttcga
     5401 tcgatgatag aacccatggt aaacgtgcat aatgctggcg catccaccac attcaccggg
     5461 atattgaggc gcttggcagc gacagacact gcttcgttta ctgcttcatc atcggtcgcc
     5521 gcaatcacca gacaggcact ggtcagttgt tccgcatgaa actcagcagg catatattca
     5581 atttcgccct gctcatacat gtctttcagc tcaatgcaca actcgggcga aatgacagtc
     5641 actttcccct gcgccttgcg cagcatgagc actttacgcg tcgctacatc gccaccacca
     5701 atgacaatac agaggcgttg agcaatattg aaaaaaatcg gtaaagcttg catgccacgc
     5761 ggacttcaaa aaataaaata gtattttaca gcagtcctca gtgttggct
//
LOCUS       DATMSK010000062         6413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_5881, whole
            genome shotgun sequence.
ACCESSION   DATMSK010000062 DATMSK010000000
VERSION     DATMSK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5881"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /gene="metG"
                     /locus_tag="VNZ84_02020"
     CDS             <1..492
                     /gene="metG"
                     /locus_tag="VNZ84_02020"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HWU65756.1"
                     /translation="DHTIQKYEALVTRLDPKQIEAMIEANKESLQAAAPVKAETNVEA
                     KAETKAAEEGAYISIDDFTKVDLRIAKIVSAEHVEGAEKLLKLLLDIGEEKPRQVFAG
                     IKSAYDPATLVGRLTVMVANLAPRKMKFGMSEGMVLAASDERGGPYILSPDSGAQAGM
                     RVK"
     gene            707..3001
                     /locus_tag="VNZ84_02025"
     CDS             707..3001
                     /locus_tag="VNZ84_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HWU65757.1"
                     /translation="MANPLAVSSANPWQGLDIQSEWERFSQRDAEAADVWTSHVWVDN
                     MHCAACSGRIEQAIKQQPGVLAVYASAASKRVRIVWNAHKTSPSQWFANLNEIGYPAL
                     PLNEYHAISDRTVQQRKMLWRLVVAVFCMMQVMMYAYPTYTAGSNDMAPDMVQLMKWA
                     SWVLTLPVMLFSATPFLSQAWASIKQRQLGMDVPVSLGIVTAFLMSTLATFAPGGWWG
                     DVVYYDSLTMFVSFLLIGRWLDVKLRNRTAGALENMMRRLPHAVLKKNEAGEWVQIAA
                     SNINVGDILFVRPGEVFAADGKVVSGNTSADESMLTGESEPVMKPAGATVIAGSGNLT
                     SAVEVQVEQVGEGTQYHALVNLMASASTEKPGIALLADKVAQPFLWGVLLAALYAVIF
                     WWTTDPAKAIMSAVAVLVVTCPCALSLATPAAMLASAGTFAKGGLLIRRLQAIQAISE
                     VDTIVFDKTGTLTENLQQVQLEESNASWDRQWLVDLIATMSRDSLHPVAKALHEFALQ
                     HDAQDKLHMQDWQEIVGGGMSATFSAPNGTLLPVKLGNAEFCGIRHEPDSQALRQVYV
                     TSGEVLLARLALSEQVKPHARAAISQLISHGLDIQLLSGDRIAPVKNLATQLGIQNAL
                     GLQKAEDKLQHVKLLQQQGRKVLMVGDGLNDGPVIAAAHTSIAMGSGVPLTQAQADMV
                     VLGNDIGVLVSLISHCKKTILIVKQNLLWAALYNAAGVPLAIMGLLPAWLAGLGMAIS
                     SLLVLLNALRLTDWDKSGWQKGQA"
     gene            3006..3143
                     /gene="ccoS"
                     /locus_tag="VNZ84_02030"
     CDS             3006..3143
                     /gene="ccoS"
                     /locus_tag="VNZ84_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HWU65758.1"
                     /translation="MEIAFLLIPLAVLLVFVILGGLWWAIQTGQFEDLEEEGSRILEH
                     D"
     gene            3267..4703
                     /gene="ccoN"
                     /locus_tag="VNZ84_02035"
     CDS             3267..4703
                     /gene="ccoN"
                     /locus_tag="VNZ84_02035"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HWU65759.1"
                     /translation="MQNATVKAATYNDTVVRQFAIMAVVWGVVGTLMGVIIASQLVWP
                     ELNLGLPFTSFGRLRPLHTNAVIFAFGGCALFATSYYVVQRTCQTRLFSDKLASFTFW
                     GWQAVIVAAAISLPLGYTQGKEYGELEWPIDILIAIVWISYAIVFFGTIGIRKVKHIY
                     VANWFYGAFILAVALLHIVESAAIPAGWFKSYSAYAGVQDAMVQWWYGHNAVGFFLTA
                     GFLGMMYYFVPKQAERPVYSYSLSIVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMV
                     LSLILFAPSWGGMINGIMTLSGAWHKLRTDPILRFLIVSLSFYGMSTFEGPMMAIKTV
                     NALSHYTDWTVGHVHSGALGWVGFISIGSLYFLIPRLFGQKQMYSVKAIEVHFWMATI
                     GVVLYIAALWISGVMEGLMWRAMNADGTLAYTFVESVKAKFPYYFTRALGGFLYMSGL
                     VIMLWNTYKTATSGKPATVEVPAVVAHA"
     gene            4714..5340
                     /gene="ccoO"
                     /locus_tag="VNZ84_02040"
     CDS             4714..5340
                     /gene="ccoO"
                     /locus_tag="VNZ84_02040"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HWU65760.1"
                     /translation="MATHEQNSGNLHEKIETNNFLLIVLTFITISFGGLVEIVPLFFQ
                     KSLTEPIRGLQPYTALQLAGRDIYLREGCYNCHSQMIRPFHAETLRYGHYSVAGEYVY
                     DHPFQWGSKRTGPDLHRVGGKYSDDWHRVHLNNPRDVVPESIMPAYPWLNENLVDDAT
                     IGDHMAALRKVGVPYTDEQIKAAGAEIKGKTEMDALVSYLQVLGTHLK"
     gene            5352..5492
                     /locus_tag="VNZ84_02045"
     CDS             5352..5492
                     /locus_tag="VNZ84_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HWU65761.1"
                     /translation="MDMINVLRILFTVLSFVLFIAILVWAWRNRHSAQFKDAQNLPFD
                     QD"
     gene            5524..>6413
                     /gene="ccoP"
                     /locus_tag="VNZ84_02050"
     CDS             5524..>6413
                     /gene="ccoP"
                     /locus_tag="VNZ84_02050"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HWU65762.1"
                     /translation="MNDFVNGFWPIWISTITLGGIAFCVGLLIFASRIKVNVQSDNTN
                     GHEWDGIKEMNNPLPMWWVGLFIITVIFGLAYLFLFPGLGTYNGKFGWTEVKQYEAEV
                     AKNNAKIAPLYAKYAAMPVEEVAKIPEARDIGKRIFLNNCAACHGSDAKGSRGFPNLT
                     DNDWLYGGAPDTIKLTINNGRNGIMPPMAAAVGSEEDVKNVANYVLSLSGSIHDSGRA
                     NAGKDKFVVCAACHGPEGKGNQAIGAPNLTDNIWLHGQGEAAIIKRIHEGKNNVMPAW
                     KEKFTPEQIHLLTAYVWGLS"
BASE COUNT         1491 a         1452 c         1760 g         1710 t
ORIGIN      
        1 gatcatacta ttcaaaagta tgaagcgttg gtgactaggc tagatccaaa gcagatcgaa
       61 gcaatgatcg aagccaataa agaaagcctg caagctgcgg cgccagttaa agctgaaact
      121 aacgtggaag caaaagccga aaccaaggct gcagaagagg gcgcgtatat ttcgattgat
      181 gactttacca aggtggattt gcgcattgct aaaattgtca gcgccgagca cgtggaaggg
      241 gcggaaaagc tgctcaagtt gttactcgat attggtgaag agaagccgcg ccaggtattt
      301 gccggtatca aatctgccta tgatccagcc acgctggtgg gtcgcctgac agtgatggtc
      361 gccaatctgg ccccacgcaa gatgaagttt ggcatgagtg aaggcatggt gctggcggcc
      421 agtgatgaac gtggtggtcc ttacatcctg tcacctgaca gtggtgctca agccggtatg
      481 cgcgtaaagt aatgtttttg tgtgctggag ctggcggcat atcatcgcgc tggctcctta
      541 taatacaagt gttgagtcac acaagttttg ttgtgttata tcaagttgtt tgtaacgctt
      601 agctggtcta atggtggttt cggaaagcat ctaacacatt gatttatatt atattcaatc
      661 atataatata aagttgtata ttgtttcgcg gatggtccat cacccaatgg caaacccttt
      721 agcagtgagc agtgcaaatc cctggcaagg actggatatc cagtctgaat gggagcgatt
      781 tagtcaacgc gatgcagagg ctgctgatgt gtggacatcg catgtctggg ttgacaatat
      841 gcattgcgcc gcgtgcagtg ggcgcataga gcaggccatc aaacaacagc ctggcgtact
      901 ggccgtatat gccagtgcgg cttccaaacg tgtgcgcatt gtctggaatg cgcataaaac
      961 ttccccctca caatggtttg ccaatctcaa cgagattggt tatcctgctt tacctctcaa
     1021 cgaatatcac gcgatttcag atcgaaccgt ccagcagcgc aagatgttgt ggcgcctggt
     1081 ggttgctgta ttttgcatga tgcaggtcat gatgtacgcc tatcccacct acacggcggg
     1141 cagcaatgat atggcaccgg atatggtgca gctcatgaag tgggcttcat gggtgttgac
     1201 cttgcctgtc atgctgtttt cggccacccc gtttttgtcc caagcctggg ccagtatcaa
     1261 gcagaggcag ttaggcatgg atgtccctgt ctctttggga attgtgacgg cgtttttaat
     1321 gagtacattg gcgacgtttg cgcccggcgg ctggtggggc gacgtcgtct actatgactc
     1381 gctgaccatg tttgtgagtt ttctgctgat tggccgttgg ctggatgtca aactgcgtaa
     1441 ccgtaccgct ggtgcgctgg aaaacatgat gcgccgttta ccgcatgctg tacttaaaaa
     1501 gaatgaggct ggtgaatggg tgcagattgc cgccagcaac atcaatgttg gcgatattct
     1561 gtttgtgcgt ccgggcgaag tatttgccgc cgatggtaag gtggtctcag ggaacacaag
     1621 tgctgatgaa tcgatgctga ctggcgaatc tgagccagtg atgaagccag cgggtgcaac
     1681 cgtgattgcc ggttcgggta acttgacctc tgctgttgaa gtgcaagttg aacaggtcgg
     1741 agaggggact cagtaccatg cgctggttaa cctgatggcc tcggcttcca ctgaaaaacc
     1801 tggcattgcc ttgctggcgg ataaagtggc ccagccattt ctgtgggggg tgttgctggc
     1861 ggcactctat gcggtgatct tctggtggac gacagaccca gcgaaagcga ttatgtcggc
     1921 tgtggcggtg ctggtggtga cttgcccttg tgccttgtca ttggcgacgc ctgcggccat
     1981 gctggccagt gccgggacgt ttgccaaagg cggcctgtta atccgccgtt tgcaggcgat
     2041 tcaggcaatc agcgaagtag acaccatcgt gtttgataaa accggcacac tgaccgagaa
     2101 cctgcagcag gtccagctcg aagaaagcaa tgcttcttgg gaccgtcagt ggttagttga
     2161 cttgattgcg accatgagcc gggactccct gcatcccgtc gcaaaagctt tacatgaatt
     2221 tgcgttgcag cacgatgcgc aggataaatt gcacatgcag gactggcagg aaattgttgg
     2281 cggcggcatg tctgctacat tttctgcgcc aaatggcaca ttactgccgg tgaagctagg
     2341 gaatgcagag ttttgtggga tccgccatga acctgatagc caggccttgc ggcaggtgta
     2401 tgttacttcc ggggaagtgt tgctggcaag gctggcttta tccgagcagg tgaagccgca
     2461 tgccagggcg gcaatttcac aattaatttc acacgggctt gatattcaac tgttatctgg
     2521 tgatagaata gcgcccgtta aaaaccttgc aacacaactg ggaatccaga atgcgttggg
     2581 gttacaaaaa gcagaagata agctgcagca cgttaagttg ctgcaacaac aggggcggaa
     2641 agttttaatg gtcggtgatg gtttgaatga cggccctgtc attgcagcag cgcatacttc
     2701 tattgcaatg ggttccggcg tccctttgac gcaggcacag gccgatatgg tggtattggg
     2761 caatgatatc ggtgtgctgg tttcgctcat ttcgcattgt aaaaaaacca tcctcatcgt
     2821 aaaacagaat ttgttatggg cggcgttgta taacgcggcc ggagtgcctt tggcgattat
     2881 gggcctgttg cctgcctggt tagccgggtt gggcatggcc atcagttcgc tgttggtgtt
     2941 gctgaatgcg ttacggttga ccgactggga taagtcgggc tggcagaaag gtcaggcata
     3001 gctccatgga aatcgcattt ttattgatcc cgctagcagt attgctggtg tttgtgattt
     3061 tgggtggcct gtggtgggcg atccagacag gtcagtttga agacctcgaa gaagaagggt
     3121 ccaggattct tgagcatgat tgaggattct tgaacacgat tgatatgtat caaatcagat
     3181 ttgtgacgtc tctgagatca tcagcgatgc acaattagaa gtttgtttta gtgttgggtg
     3241 tttgttttaa tcattgggag aaatccatgc aaaatgcaac cgtgaaggct gcgacctaca
     3301 acgataccgt ggtccgccag ttcgcaatca tggcagtggt ctggggtgtt gttggtacat
     3361 tgatgggcgt gattatcgcg tctcagctgg tgtggccgga actgaacctg ggcctgccgt
     3421 ttacctcttt tggccgttta cgaccattgc ataccaatgc agtgattttc gcatttggtg
     3481 gctgtgcttt atttgctacc tcatactatg tcgttcaacg tacctgccaa acccgtttgt
     3541 ttagcgacaa actggccagt ttcaccttct ggggctggca agctgtgatt gttgccgcag
     3601 ccatctcctt gccattgggt tatacccaag gtaaagagta cggcgaactc gagtggccaa
     3661 ttgacatcct gattgcaatc gtatggattt cttatgccat cgtgttcttc ggtaccatcg
     3721 gtatccgcaa ggttaaacat atttacgtgg ctaactggtt ctacggtgcg tttattctgg
     3781 cagtggcgtt gttgcacatt gttgagagtg cggcgatccc ggctggctgg tttaagtctt
     3841 actctgctta cgcaggtgtg caagatgcga tggtgcagtg gtggtatggt cacaacgcgg
     3901 tgggtttctt cctgacagcg ggcttcctgg gcatgatgta ctactttgtg cctaaacaag
     3961 ctgagcgtcc tgtgtactct tacagcttgt ctatcgtgca cttctgggca ctgattttca
     4021 cttacatgtg ggcgggtcct caccacctgc attacaccgc attgcctgac tggactcagt
     4081 ctctgggtat ggtgttgtct ttaatcctgt ttgcaccaag ctggggcggt atgatcaacg
     4141 gtatcatgac cttgtccggc gcatggcaca aactgcgcac agatcctatc ttgcgcttcc
     4201 tgatcgtttc cctgtctttc tacggcatga gtacctttga aggtcctatg atggcgatca
     4261 agactgtaaa cgctttgtct cactacactg actggactgt tggtcacgtg cactctggtg
     4321 ctttgggctg ggtaggtttc atttctatcg gttccttgta cttcctgatt cctcgcctgt
     4381 ttggccagaa acaaatgtac agcgtgaaag ccattgaagt gcacttctgg atggcaacca
     4441 ttggcgtggt gttatacatc gctgcgttgt ggatctccgg cgtgatggaa ggtttgatgt
     4501 ggcgcgcaat gaacgctgac ggtactttgg cctacacctt cgttgaaagt gtaaaagcga
     4561 agttccctta ctacttcaca cgtgcattgg gtggcttcct gtacatgagc ggcctggtca
     4621 tcatgttgtg gaatacatat aaaacagcaa ctagcggcaa accagcaacg gtagaagtac
     4681 cagctgttgt tgcacacgct taaggaattc actatggcaa ctcatgaaca aaatagtggc
     4741 aatctgcacg aaaaaattga aaccaataac ttcctgttaa tcgtgctgac tttcatcacg
     4801 atctcctttg gtggtctggt tgaaatcgtg cctctgtttt tccaaaaatc tctgaccgag
     4861 cctatccgtg gtttgcagcc gtataccgcg ctgcaactgg ctggccgtga catttatctg
     4921 cgtgaaggct gctacaactg ccactcacag atgatccgtc cgttccatgc tgaaacacta
     4981 cgttacggcc attactctgt cgcaggtgaa tacgtgtatg accacccatt ccagtggggt
     5041 agtaagcgta caggtcctga cctgcaccgt gtaggcggaa aatacagtga cgactggcat
     5101 cgtgttcacc tgaacaaccc acgtgacgtg gttccggaat ccatcatgcc tgcgtatcca
     5161 tggttgaatg aaaacctggt agacgatgca acgattggtg accatatggc tgcgctacgc
     5221 aaagtgggcg tgccttacac cgatgagcag atcaaggctg ctggcgcaga aatcaaaggt
     5281 aaaaccgaga tggatgcact ggtttcatac ctgcaagtac tgggtacaca cctgaaataa
     5341 ggagacgcgc catggatatg atcaatgtat tacgcatatt gtttacagta ctgagtttcg
     5401 tgctgtttat cgcaattctg gtctgggcat ggcgcaatcg tcactccgca cagttcaagg
     5461 acgcgcaaaa cctgccgttc gatcaagact aaatacctgt gcgggcatgt taaggaaaac
     5521 atcatgaatg attttgtaaa tggattctgg ccgatctgga tttcgaccat taccttgggc
     5581 gggattgctt tttgcgtagg cttactgatt tttgccagcc gcattaaagt gaatgtgcaa
     5641 agtgacaaca ccaatggtca cgagtgggat ggtattaaag agatgaacaa cccgctgcca
     5701 atgtggtggg taggcttgtt cattatcacg gtgatctttg gtttggctta cttgttcctg
     5761 ttcccaggcc tgggaactta caacggtaaa ttcggctgga cagaagtcaa acagtacgaa
     5821 gctgaagttg ccaagaacaa cgcaaaaatt gcaccgttat atgccaaata tgctgcgatg
     5881 ccagtggaag aagtcgccaa gattccggaa gcacgggata tcggtaagcg tatcttcctc
     5941 aacaactgtg cagcttgtca cggttcagat gccaagggta gccgcggttt ccctaacctg
     6001 acggacaatg actggttgta tggtggtgca ccggatacca tcaaactgac tatcaacaat
     6061 ggccgtaacg gcatcatgcc accgatggct gctgctgttg gctctgaaga agatgtcaaa
     6121 aatgtggcta actatgtact gagtttgtct ggcagcattc atgactccgg tcgtgccaat
     6181 gccggtaaag acaagtttgt tgtttgtgca gcttgtcatg gtccagaagg caaaggtaac
     6241 caggcaattg gtgcgcctaa cctgacagac aatatctggt tgcatggcca gggtgaagcc
     6301 gccattatca agcgtattca cgaaggtaaa aacaacgtaa tgccggcctg gaaagaaaaa
     6361 tttacacctg agcaaattca tttgctgacc gcttatgttt ggggcctttc caa
//
LOCUS       DATMSK010000063         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_216934,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000063 DATMSK010000000
VERSION     DATMSK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216934"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VNZ84_02055"
     CDS             complement(<1..491)
                     /locus_tag="VNZ84_02055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWU65763.1"
                     /translation="MHPTLNVAIRAAREAGKIINRAALDIGALKIEQKDTNDFVSEVD
                     RNAEQAIIDVLKDAYPTHGFYGEESGKSNVESDSIWIIDPLDGTTNFLHNFPAYCISI
                     ALQEKGVLTQAVIYDPVRNDLFTATKGSGAFLNNRRIRVTQRAKLQEALISTGFPFKD
                     FSY"
     gene            575..1327
                     /locus_tag="VNZ84_02060"
     CDS             575..1327
                     /locus_tag="VNZ84_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWU65764.1"
                     /translation="MSQTTESSIFANIRVVLCQTSHPGNIGSTARAMKTMGLSRLYLV
                     RPKHFPDGEAKSLAVNAADLLDSAVVTETLEEALADCQFVIGVSGKERSLSQQVMTVR
                     EAAQEVKAVASHSQVALVFGTEMSGLSNAEADRCHVLATIPANPEYTSLNLAQAVQIM
                     CYETRMAVTTGELHYSEKPVELATQEDLERFYDHMREVLEHIGYINPRAPKKLFERLR
                     RLYGRTRLEKEEVNLLRGILTLTVQPKKHTKY"
     gene            complement(1324..1638)
                     /locus_tag="VNZ84_02065"
     CDS             complement(1324..1638)
                     /locus_tag="VNZ84_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HWU65765.1"
                     /translation="MSAACQSCGACCANFRVSFYWAETDAHPLGRVPAELTRKINDTY
                     VCMKGTEQNPVRCIALQGEVGKSVGCRIYSLRSSTCREFDAGSEVCNQAREKIGLAPI
                     AA"
     gene            1739..>2138
                     /gene="epsC"
                     /locus_tag="VNZ84_02070"
     CDS             1739..>2138
                     /gene="epsC"
                     /locus_tag="VNZ84_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HWU65766.1"
                     /translation="MFNHLKEDISIVFDRDPAARTHFEILTTYPGVHALILHRVSHWF
                     WSHRLYWLGRALSHFGRWITGIEIHPGATIGRRVFIDHGMGVVIGETAVIGDDCTLYH
                     GVTLGGTSWNKGKRHPTLESGVVIGAGAKVL"
BASE COUNT          559 a          559 c          522 g          498 t
ORIGIN      
        1 aagtagctaa agtctttgaa cggaaacccg gtagaaatca acgcctcctg taatttggca
       61 cgctgcgtca cacgaatgcg ccggttattc aaaaacgcac cactgccttt ggtagcagta
      121 aataagtcat tacgtaccgg atcataaatc acggcctggg tcagtacgcc tttttcctgc
      181 aaggcgatag aaatacagta ggccgggaag ttgtgcagga agttggtggt accatcgagc
      241 gggtcaataa tccagatact gtcagactcg acattggact taccgctttc ctcgccataa
      301 aagccatgcg ttgggtaggc atctttcaat acgtcgataa tagcttgctc agcattgcga
      361 tcaacttcac tgacaaagtc gttagtatct ttttgttcga tttttaaagc gccaatatcg
      421 agcgcggcac ggttgatgat tttgcctgct tcacgcgcgg cgcgaatggc gacattcagg
      481 gttggatgca tgctgtgtga tcttgattca actgataaaa tgctatttta gctttttaca
      541 cgcttagcca caaacacaac ggcaacattt atccatgtca cagactaccg aatcctccat
      601 atttgcaaac atccgcgtcg tactctgcca aaccagccat cctggcaaca taggctcgac
      661 cgcacgcgcc atgaaaacaa tgggcctcag ccgcctttac ctggtacggc ccaaacactt
      721 tccagatggc gaagcaaagt cgctagcagt gaatgccgca gatttgctag acagtgcggt
      781 agtgactgaa accttggagg aggcgctggc cgattgccag tttgtgattg gtgtcagtgg
      841 caaggagcgg tcactctcgc aacaggtcat gaccgtacgt gaagccgcac aagaagtgaa
      901 agccgtggcg agtcacagcc aggtcgcgct ggtatttggc accgaaatga gtggcctgag
      961 caatgccgaa gccgaccgtt gccatgtgct tgccaccatt ccggccaatc ccgaatacac
     1021 ctcactcaac ctcgcccagg cagtacagat catgtgttat gaaacacgta tggcagtgac
     1081 cacaggtgaa ttacattaca gcgaaaaacc agtcgaactt gccacgcaag aagatctgga
     1141 acgcttttac gaccatatgc gtgaagtgct ggaacatatc ggctatatta atccccgggc
     1201 accgaaaaag ctgttcgagc gactgcgtcg cctctatggc cggacacgat tggaaaaaga
     1261 agaagtgaat ttactacgcg gcattctgac actgactgtg cagccaaaaa aacataccaa
     1321 gtactaagca gcgataggtg ccaggccgat tttttcccgc gcctggttgc aaacctctga
     1381 cccggcgtca aactcgcggc aggtactgga acgtaaagaa taaatccggc aacctacaga
     1441 tttgccaact tccccttgca acgcgataca gcgaaccgga ttctgctcag tccccttcat
     1501 gcaaacatag gtgtcattaa ttttcctggt caactccgca ggcacccttc ctaaaggatg
     1561 cgcatccgtc tcagcccaat aaaaagagac ccgaaaattg gcgcagcaag cgccgcaaga
     1621 ctgacaagca gcagacatga gaatgttggc aaaaattaag cttttatttt aaattgaccg
     1681 cctcactcac gccaatgtga ttttcagccg tactaacaac ttatggcata atgcgcccat
     1741 gttcaatcac ctcaaagaag atatttcaat tgtgtttgac cgcgaccctg cggcacgcac
     1801 acactttgaa attctgacaa cctaccccgg tgtgcatgcc ctgattttgc atcgtgtaag
     1861 ccattggttt tggagtcacc gcctgtattg gctgggtcgc gccctttccc acttcggacg
     1921 ctggattacg ggtattgaaa tccaccccgg cgcaacgatt ggccgccgcg tatttattga
     1981 tcacggcatg ggtgtggtca ttggtgaaac tgcggtgatt ggggatgatt gcacgctgta
     2041 tcatggtgtc acactgggtg gcaccagctg gaacaaaggc aaacgtcacc ccacgctgga
     2101 atctggtgtg gtgattggtg ccggcgccaa agtgctgg
//
LOCUS       DATMSK010000064        10458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_193763,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000064 DATMSK010000000
VERSION     DATMSK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10458
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193763"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..241)
                     /locus_tag="VNZ84_02075"
     tRNA            complement(148..241)
                     /locus_tag="VNZ84_02075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(205..207),aa:Ser,seq:gct)
     gene            complement(399..1628)
                     /locus_tag="VNZ84_02080"
     CDS             complement(399..1628)
                     /locus_tag="VNZ84_02080"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWU65767.1"
                     /translation="MALIVQKYGGTSVANPERIRNVARRVARYKALGHQVVVVVSAMS
                     GETNRLISLAKEIMPDPDPRELDVMVSTGEQVTIGMTALALMELGIKAKSYTGTQVRI
                     LTDDAYSKARILDIDERNLKKDLDDGYVCVVAGFQGVDANGNITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRLEKITFEEMLELASQGSKVLQIRSVEF
                     AGKYKVKLRVLSSFEEEGDGTLITFEENEENMEEPIISGIAFNRDEAKITVTGVPDKP
                     GIAYQILGPVADANIDVDMIIQNVGADGTTDFTFTVHKNEMNKALSILRDKVQGHIQA
                     REISGDDKIAKVSVVGVGMRSHVGIASQMFRTLAEEGINIQMISTSEIKIAVVIEEKY
                     MELAVRVLHKAFGLENA"
     gene            complement(1659..4295)
                     /gene="alaS"
                     /locus_tag="VNZ84_02085"
     CDS             complement(1659..4295)
                     /gene="alaS"
                     /locus_tag="VNZ84_02085"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWU65768.1"
                     /translation="MTVKYGQKPSSKQIRQAFLDFFASKGHQVVASSSLVPHGDPTLL
                     FTNAGMNQFKDVFLGFDKRAYTRAATAQKCVRAGGKHNDLENVGYTARHHTFFEMLGN
                     FSFGDYFKRDAITFAWELLTEVYALPKERLMVTVYAEDDEAYDIWNKEVGVPADKIVR
                     IGDNKGARYASDNFWMMGDTGPCGPCTEIFYDHGAHIPGGPPGSPDEDGDRFIEIWNN
                     VFMQFNRDETGVMHLLPKPSVDTGMGLERISAVLQGVHANYEIDLFQALIKAAARETN
                     TADLESPSLKVLADHIRACSFLIADGVIPGNEGRGYVLRRIIRRAIRHGYKLGYRAAF
                     FHKLVADLVAEMGEAYPELVSNQARITDILKLEEDRFFETIENGMQILEAELATKPAV
                     FNGDLAFKLHDTFGFPLDLTADICRERGVTVDTAGFDAAMARQKEQARAAGKFKMALN
                     LEYEGAATTFHGYDKLETPAKVLALYKDGSAVQTLNEGDLGVVVLDHTPFYAESGGQI
                     GDSGELKSANGIFAVEDTQKIQAAVFGHHGVLKTGTLSVGDDLTAKVNLQARASTMRN
                     HSATHLMHKALREVLGEHVQQKGSLVDTEKTRFDFVHNAPMTDAEIAKVEALVNAEIL
                     ANVATEARLMDIESAQKTGAMMLFGEKYGDEVRVLDIGSSRELCGGTHVSRTGDIGLF
                     KITSESGVAAGVRRVEATTGEGALKLVQAQQMLINQVAGELKAPAHELPAKIAQVMDN
                     VKSLEKELARLKSKLASSQGDDLATQAQDVNGAKVLAATLEGVDANALRETMDKLKDK
                     LKSAVIVLASVSDGKVSLAAGVTADLTSKVKAGELVNHVAGQVGGKGGGKPDMAMAGG
                     TEPANLPQALASVKAWVEKKQN"
     gene            complement(4285..4743)
                     /gene="recX"
                     /locus_tag="VNZ84_02090"
     CDS             complement(4285..4743)
                     /gene="recX"
                     /locus_tag="VNZ84_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HWU65769.1"
                     /translation="MRQPLEKSLRQRALEYLSKREYSSAELAQKLKSYADEADDIPAL
                     IADFKMRGWLSDARYAEQMVHARQAKFGATRVAHELREKGVDDILIAEAVAGLQGSEV
                     ERASEVWRKKFKAAPITREEWAKQARFLQSRGFTFEVIKQILNRQVEDDS"
     gene            complement(4749..5777)
                     /gene="recA"
                     /locus_tag="VNZ84_02095"
     CDS             complement(4749..5777)
                     /gene="recA"
                     /locus_tag="VNZ84_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWU65770.1"
                     /translation="MDDNKSKALAAALSQIEKQFGKGSIMRMGDADIGEDLQVVSTGS
                     LGLDIALGVGGLPRGRIVEIYGPESSGKTTLTLSAIAEMQKLGGTAAFIDAEHALDPQ
                     YAAKLGVNVPDLLISQPDTGEQALEIADMLVRSGSVDIVVVDSVAALTPRAEIEGEMG
                     DSHMGLQARLMSQALRKLTGNIKRTNTLVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     SSVRLDIRRTGAIKKGDEVIGSETKVKVIKNKVAPPFKQAEFDIMYGEGISRLGEVIE
                     LGTNLKLVEKSGAWYSYNGEKIGQGKENAKEFLRENPAIAAEIEAKIRENSNVLADGM
                     AAARGEDD"
     gene            6009..6350
                     /locus_tag="VNZ84_02100"
     CDS             6009..6350
                     /locus_tag="VNZ84_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65771.1"
                     /translation="MKIAKYLLGLALGSMAAFNAQAGDHWSVGVAIGNPYPPAYYAPP
                     PVYYAPPPVVIYRHPQSAYYGVPAVAYPQPAPGFIQFSYGNGGYGGYNYGPRNFNGGG
                     WGRGHGHHHHH"
     gene            complement(6354..6863)
                     /locus_tag="VNZ84_02105"
     CDS             complement(6354..6863)
                     /locus_tag="VNZ84_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HWU65772.1"
                     /translation="MTNALLELSAALGQALREKGWQLSLAESCTGGMIAQSVTAIAGS
                     SAWFDRGFVTYSNAAKTDMLAVPEALIVEYGAVSEPVAEAMAQGALTHSTATLSGAVT
                     GVAGPGGGTPQKPVGTVCFAWAVRGQVGQVDLMRIETCHFDGDRTQVREQAASYLLAG
                     LLQLATSKL"
     gene            complement(6860..7333)
                     /locus_tag="VNZ84_02110"
     CDS             complement(6860..7333)
                     /locus_tag="VNZ84_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HWU65773.1"
                     /translation="MQPGWRLLFSHPLHCLSFGLGTGLSPKAPGTVGTLLGFPLYWLL
                     TGLPLSQQWLILAVLFLFGIWCCEFTGRALGVSDHGAIVWDEVVAMAAVLMTVPQTFI
                     WGLAAFASFRLFDIWKPFPISWADRHVKGGFGVMLDDALAAALAVFVLHLVQRVV"
     gene            complement(7350..8297)
                     /gene="thiL"
                     /locus_tag="VNZ84_02115"
     CDS             complement(7350..8297)
                     /gene="thiL"
                     /locus_tag="VNZ84_02115"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWU65774.1"
                     /translation="MAEFDIIAKYFTRPAQADVGIGDDAALIRVRAGYQLAVSADMSV
                     AGTHFFADIDPFAIGWKAMAVNVSDMAAMGAEPKWATLSIALPDINHTWLQGFSAGLF
                     ACADAYGVSLIGGDTTRGPLNIAINIFGEVPQGQALQRNGAQTGDDIWVSGELGCAAL
                     WLQHRLGNLHLYAEELSMLAEAMHHPQPRIGLGLALRGIATAALDISDGLLADLGHIL
                     RASGTGAILDWQAIPKPELQRSEISESLLQQVVLAGGDDYELCFTAPVLQRDYLHGLS
                     DQLGLSLSRIGQITEGTELQVLDGQVPMVLSQKGYEHFG"
     gene            complement(8290..8646)
                     /locus_tag="VNZ84_02120"
     CDS             complement(8290..8646)
                     /locus_tag="VNZ84_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HWU65775.1"
                     /translation="MFYKTTYKLIFILCLAVLTYLLLMEMAPTNDGSLYKDKVQHIVA
                     FGGVAFWGLLAFQRYFKSVMLGLTVFGALMEVLQGVLTTTRQPSVFDWVADVVGILLA
                     WGVVIAWQRWWNGRRG"
     gene            complement(8654..9274)
                     /gene="mog"
                     /locus_tag="VNZ84_02125"
     CDS             complement(8654..9274)
                     /gene="mog"
                     /locus_tag="VNZ84_02125"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HWU65776.1"
                     /translation="MQEQITRIGLISISDRASQGIYEDKGIPALTAWLAQALISPYTL
                     ATRLIADDQGTISRTLEQLVDEEACHLILTTGGTGPALRDVTPEATLAVADREMPGFG
                     EQMRQISLRFVPTAILSRQVAVIRKQCLIINLPGQPKSIQETLEGLRDEEGKSVAPGI
                     FAAVPYCLDLLATPDRPMPYLETDETVVKSFRPKSAIRPPATRASN"
     gene            9353..10159
                     /locus_tag="VNZ84_02130"
     CDS             9353..10159
                     /locus_tag="VNZ84_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWU65777.1"
                     /translation="MNIPYNHGQLATSASVRRNPLPAPSLLWLTILLVAATLLASPAT
                     RAEDNPYASNYQAQNQGNLHSMQANPEPQLFSGTRRDEDNIKMLENGYDLMGFSSFEA
                     EDVPTEQVISHGRTIRADSILVYVKKAGNTTPASKIEMIKEASRKGIALTEKDMAVDP
                     TKYRYYATYWAKLPPPVLGVHVIKLVPRSSNEKEGPSVSSDGVRVIAVIHGSAAEKAG
                     LLRGDQLLSINQEKVQDAAELSTLVRKYRGKRVQLQLERQDEPIQLEAQL"
     gene            10217..>10458
                     /locus_tag="VNZ84_02135"
     CDS             10217..>10458
                     /locus_tag="VNZ84_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit TatB"
                     /protein_id="HWU65778.1"
                     /translation="MFQPIFDHQTAREAAGDINDIEFIRALFDNIRPKESTDIESASN
                     ALQALCFLLQQQPQFAMLLRNSLLRLLNEKTVISLYS"
BASE COUNT         2638 a         2922 c         2368 g         2530 t
ORIGIN      
        1 tactctatcc aactgagcta cgggcgccaa ggtttgttgc tttgcaacaa agacagcatt
       61 ataacactaa attaagcttt gtgcctagcc agattaccat ctggctactg attaaccaaa
      121 aacaaaactc gcacaacgcg aggtttttgg cggtgagcga gggattcgaa ccctcgatac
      181 aggtttaagc ccatatgctt ccttagcagg gaagtgcctt cgaccactcg gccagctcac
      241 cttattaaaa ccttacagct ttaataatac caacacagct tacgctgccc tctttgcagc
      301 attgctactg catcgaaggg cgccatacta cgtgaaaacc cttcaaaaat caacgcaaaa
      361 aagaagaatg ttttatttct ttatttttgc atttgcgatt aggcgttttc aaggccgaac
      421 gctttatgta acacgcgcac agccagttcc atatacttct cttcgatcac cacggcgatt
      481 ttaatttcac tggtagaaat catctggata ttaatgccct cttctgccag tgtacggaac
      541 atctggcttg caatgcctac gtgtgaacgc atcccgacgc cgacgacaga gactttggca
      601 attttgtcgt caccgctgat ctcacgtgcc tggatatggc cttgcacttt atcccgcagg
      661 atgctcaagg ctttgttcat ctcgttttta tgcacggtaa aggtaaagtc ggttgtgcca
      721 tccgcaccca cgttctggat aatcatatcc acatcgatat tggcatcagc cacaggaccc
      781 agaatctggt aagcaatccc tggtttatcc ggtacgccag tcacagtaat tttcgcctca
      841 tcgcgattaa aagcgatgcc ggagataatt ggttcttcca tgttttcctc attttcttcg
      901 aatgtgatca gtgtaccgtc gccctcttct tcaaagctgg acagcacacg taatttgact
      961 ttgtatttac cggcaaactc gactgatcga atttgcaata ccttggaacc ttgcgaagcc
     1021 agttccagca tttcttcaaa ggtaatcttc tccaggcgcc gtgcttcagg caccacgcgt
     1081 ggatcagtgg tgtaaacgcc gtccacatcg gtataaatct ggcactcatc ggcctttaat
     1141 gccgcggcca gcgccacgcc tgtggtatct gagccaccac gacccagggt agtgatgttg
     1201 ccattggcat caacgccctg gaaaccagcc acgacacaga catagccgtc atcgaggtcc
     1261 tttttcaggt tgcgctcgtc aatatccaga atacgcgctt tgctataagc atcatcggtc
     1321 aggattctga cctgcgtgcc agtatagctt ttagctttaa tacccagttc catcagcgcc
     1381 agtgccgtca tgcctatggt aacttgttca ccggtagaga ccataacatc cagttcacgt
     1441 ggatccggat ccggcataat ttccttggcg agggaaatca aacgattagt ttcgccggac
     1501 atggctgaaa ccaccaccac aacttgatgc cccaacgctt tgtatcgcgc cacacgacgg
     1561 gccacgttac ggatacgctc gggatttgcc accgaggtac caccatattt ttgtacgatt
     1621 aatgccatta tttcgctctg ctaatcaatt aaattaattt agttttgttt cttttctacc
     1681 caagccttca cgctcgccaa agcctgcggc aaattcgcag gctctgtgcc acctgccatt
     1741 gccatatctg gtttgccacc gcctttgcca cccacctgcc cggcgacatg gttaaccagc
     1801 tccccagcct tcaccttgct agtcaggtct gcagtcacgc cagcagctag gctgactttg
     1861 ccgtcagaca cactcgccaa cacaatgacg gcagatttga gtttatcctt gagcttatcc
     1921 atagtttcac gcaaggcatt ggcatccacc ccttccaggg tagctgccaa caccttcgcg
     1981 ccattaacat cttgggcttg tgtcgcaagg tcgtcgccct gagaagaagc cagtttagac
     2041 ttcaggcgcg ctaattcttt ttcaagtgat ttcacgttat ccatcacttg cgcgatctta
     2101 gccggcagct catgtgcggg tgccttgagc tcaccagcca cctgattaat caacatttgc
     2161 tgtgcctgta ccaatttcaa ggcgccttcg cctgtggttg cttccacacg tcggacaccg
     2221 gcggcaacgc cgctttctga cgtaattttg aagaggccaa tatcgccggt gcggctgaca
     2281 tgggtaccac cacataactc tcgtgaggat ccaatatcga gtacacgcac ttcatcgccg
     2341 tatttctcgc caaacagcat catggccccg gttttttgtg cggactcaat atccatcagg
     2401 cgcgcttcgg ttgccacgtt ggcgaggatt tcagcattga ccagcgcctc gacttttgca
     2461 atttcggcat cggtcatcgg cgcattatgt acaaagtcaa aacgagtttt ttcagtatcg
     2521 accaagctgc ctttttgttg cacatgctcg cccagcactt cacgcaaggc cttgtgcatc
     2581 agatgggtcg ccgagtggtt gcgcatggtg ctggcacgcg cttgcagatt gaccttggct
     2641 gttaaatcat cacccacaga caaggtgccg gttttaagca caccgtggtg accaaacacg
     2701 gcggcctgaa ttttttgtgt atcttccacg gcaaaaatac cgttagcaga tttgagttcg
     2761 ccgctatcgc caatctgacc gccggattcg gcatagaaag gcgtatggtc gagcacaacc
     2821 actcccaaat caccttcatt caatgtttgt acggcactgc catctttata caacgccagc
     2881 actttggcgg gagtttccag tttgtcatag ccgtggaatg tagttgcagc gccctcgtat
     2941 tccaggttca gcgccatttt gaatttaccg gcagctcgcg cctgttcttt ttggcgcgcc
     3001 atggcagcat caaaacccgc cgtatctacc gtaactccac gctcacggca aatatctgcc
     3061 gtcaaatcca gcgggaaacc aaaggtatca tgcagcttaa acgccaggtc accattaaat
     3121 acggctggtt tggtcgccag ttcggcttcc aaaatctgca tgccgttttc aatggtttcg
     3181 aagaagcgat cctcttcaag cttgagaata tcggtgatac gtgcctgatt gctgacgagt
     3241 tcgggatagg cttcccccat ttcagccacc aaatcggcaa ccagtttgtg gaagaatgca
     3301 gctctgtagc ctaatttgta accgtgacga attgcacggc gaataatccg gcgtaacaca
     3361 tacccacggc cttcgttgcc gggaatcacg ccatctgcaa tcaggaagga acaggcacgg
     3421 atatgatccg ccagcacttt caatgaaggg ctttccagat cagccgtatt cgtttcgcgg
     3481 gcagctgctt taatcagcgc ctggaacaaa tcaatttcat agttggcatg cacgccttgc
     3541 aacacggcag agatacgctc caagcccatg ccggtgtcga cggatggctt gggtaataga
     3601 tgcatcacgc ccgtttcgtc gcggttaaac tgcataaaca cgttgttcca gatttcgatg
     3661 aaacggtcac cgtcttcgtc tggggagcct gggggcccgc ctggaatatg tgcgccgtgg
     3721 tcatagaaaa tttcggtaca agggccgcaa ggaccggtgt cccccatcat ccagaagtta
     3781 tcggaagcat agcgcgcacc tttgttgtca ccaatacgga caatcttgtc tgcgggcacg
     3841 cccacttctt tgttccagat atcgtaggct tcgtcatcct cagcgtacac agtcaccatc
     3901 aggcgctctt tgggtaaggc gtatacctca gtcagtaact cccaggcaaa ggtgatggca
     3961 tcacgcttaa aataatcgcc aaagctgaag ttccccagca tttcgaagaa tgtatgatga
     4021 cgggcggtat agccaacgtt ttccagatcg ttatgcttgc cgccagcacg gacacatttt
     4081 tgcgctgtcg ccgcacgtgt ataggcacgc ttgtcaaagc cgaggaacac atccttaaac
     4141 tggttcatgc cagcgttggt aaacagcaag gtggggtcac catgcggcac cagtgaactg
     4201 gaagctacca cctgatggcc tttggaagca aagaaatcca ggaaggcctg gcggatttgc
     4261 ttgctggaag gtttttgccc gtacttaact gtcatcttct acttgtctat tcaaaatttg
     4321 tttaatcact tcaaacgtaa aaccccgact ctgtaaaaaa cgggcctgtt ttgcccattc
     4381 ctcgcgcgtt atcggggctg ctttaaactt tttgcgccag acttcgctgg cgcgttcaac
     4441 ttcactgcct tgcaaaccgg caacggcttc tgcgatcaag atatcgtcta cccctttttc
     4501 gcgtagctca tgcgccaccc gtgttgcacc aaacttagcc tgacgcgcat ggaccatttg
     4561 ctctgcgtag cgtgcgtcac ttaaccagcc acgcatctta aagtctgcga tcaaggctgg
     4621 aatatcatcc gcctcgtctg cataactttt cagcttttgc gccagctcgg ccgaagaata
     4681 ttcacgctta cttagatact ccaacgcacg ttggcgcaac gatttttcga gcggctggcg
     4741 caaatggatt aatcgtcctc acctctggca gcggccatgc catctgccag cacattagag
     4801 ttttcacgaa tcttggcctc aatttctgcc gcaatcgctg gattctcacg caagaactct
     4861 ttagcgtttt ctttgccctg gccgatcttc tcaccgttgt agctgtacca cgcacctgat
     4921 ttttcaacca gtttcaggtt ggtacccaac tcaatcactt cgcccaaacg ggaaataccc
     4981 tcgccataca taatgtcaaa ctcagcctgt ttgaacggcg gtgccacttt atttttgatg
     5041 actttgacct tggtttcaga accaatcact tcatcaccct ttttgatcgc gccggtacgg
     5101 cggatatcca gacggacaga tgagtaaaac ttcagcgcgt taccgccagt ggtggtttcc
     5161 gggttgccga acatgacgcc gattttcata cggatctggt taataaaaat caccagcgta
     5221 ttggtacgtt tgatgttacc ggtcagtttg cgcaaagctt gtgacatcag gcgtgcctgc
     5281 aagcccatgt ggctgtcacc catttcacct tcaatttcgg cacgcggcgt caaagcggct
     5341 acagagtcaa cgaccacgat atcaacagaa ccggaacgta ccagcatatc ggcaatttcc
     5401 agcgcctgtt cacctgtatc tggttgtgaa atcagcaaat ccggcacatt cacacccaac
     5461 ttggccgcgt attgcggatc cagtgcatgc tcagcatcga taaaagcagc cgttccgccc
     5521 agcttttgca tctcggcaat ggcggacaag gtcaatgtgg ttttaccgga agattccggg
     5581 ccgtagattt caacaatccg cccgcgtggc aacccgccta cgcccagtgc aatatccaaa
     5641 cctaatgagc cagtggaaac cacttgcagg tcttcgccga tatcagcatc ccccatgcgc
     5701 atgatggagc ctttgccgaa ctgtttttca atttgagaga gtgcggcggc gagtgctttg
     5761 cttttgttgt catccatgct gattgccttc ttttagcgat aaaaatgcgc tgtttacaca
     5821 aacgctatat aatccaaaca ataacttcta attatttcat aaatcgacca tcagtgaaat
     5881 ggcgaagcca tgaattagca ccagtgattt cggcttgaaa attcagcctg aaacttgtca
     5941 accatgaggc aagtcacacg ttaagtgaat ctgggagcat taatttgttt tccgcgtgag
     6001 gagaatttat gaaaatcgca aaatacttat tagggttagc actaggcagc atggctgctt
     6061 tcaatgcgca agccggagat cactggtctg tgggtgtcgc tatcggcaat ccatatcctc
     6121 ctgcttacta cgcaccacca cccgtttatt atgcaccacc accagtggtg atctaccgtc
     6181 acccacagag cgcttattat ggggttcctg cggtcgccta tccacaacct gcgccaggct
     6241 ttatccagtt ttcttatggt aacggtggct atggtggtta caactatggt ccaaggaatt
     6301 tcaacggtgg cggctggggc cgtggtcacg gtcaccatca tcaccactaa tttttaaagt
     6361 ttagaagtgg ctaactgtag caagcctgcc agaagatagc tggcagcttg ctcccgcacc
     6421 tgtgtgcggt caccgtcgaa atgacaggtt tctatccgca ttaaatcaac ttgacctact
     6481 tgaccgcgca cagcccaggc aaaacacaca gtgcctaccg gcttttgcgg ggtgcctccc
     6541 ccaggaccgg caacgcctgt cactgcgcca ctgagcgttg ctgtgctatg tgttaatgcc
     6601 ccttgtgcca tcgcctcggc gacaggctca ctgaccgcac catattccac aatcagcgct
     6661 tccggcaccg ccagcatatc ggttttcgcc gcattgctat aggtcacaaa cccacggtcg
     6721 aaccacgccg agctacccgc aatagcggtg acagattgtg caatcatgcc gcccgtacaa
     6781 gattcagcga gcgacaattg ccagcccttt tcccgcaagg cctgccctaa tgcagcactc
     6841 aattccagca aagcgttcgt cataccactc tctgcacaag atgtaataca aacacagcca
     6901 aagcggcagc cagcgcatca tccagcatga caccaaaacc acctttgacg tggcgatccg
     6961 cccagctaat cgggaaaggc ttccagatat caaacaggcg gaaactggca aaagccgcca
     7021 ggccccaaat aaaggtctga ggcaccgtca ttaacactgc cgccatggcc accacttcat
     7081 cccaaacgat cgcaccatga tcactgaccc ccagcgcacg gccagtaaac tcgcagcacc
     7141 agatcccaaa caggaaaagc acggccagga ttaaccactg ctgacttaaa ggcaagccgg
     7201 ttaatagcca gtaaagcgga aaacctaata aggttccgac agtccctggt gctttaggtg
     7261 acaaaccagt gcctagacca aaactcaggc aatgcaaagg atgcgagaac aacaagcgcc
     7321 agcctggttg cattgtttgc cgaccttgat taaccaaaat gctcgtatcc tttctgtgac
     7381 agcaccatag gcacttgtcc atccaacacc tgcaattcag taccctcggt gatttgtccg
     7441 atccggctta acgacaagcc gagttgatcg gacagcccat gcaagtaatc cctttgcaaa
     7501 acgggtgcgg taaaacacaa ttcataatca tcgccgcccg ctaaaacgac ttgctgcaat
     7561 agggactcgc ttatctctga tcgctgtaat tccggtttgg gtatcgcctg ccaatccaag
     7621 atggctcctg tgccagaagc ccgcaaaata tgccctaaat ccgctagcaa gccatcagaa
     7681 atatctaatg ccgccgtcgc aattccacgt aatgccaatc ctaagccgat gcggggttgt
     7741 ggatggtgca tggcttccgc cagcatggaa agctcctccg catacaaatg cagattaccc
     7801 aagcgatgtt gtagccataa tgcagcacaa cctaactcgc cgctgaccca gatatcatca
     7861 ccggtttgcg cgccattgcg ctgcaaagcc tgcccctgtg gcacctcacc aaaaatgtta
     7921 atggcaatat tcaaaggccc ccgcgtagta tcgccgccga tcaacgaaac accataagca
     7981 tccgcacaag caaacaggcc agcactaaac ccctgcaacc acgtgtgatt aatatcgggc
     8041 aaagcaatcg ataaagtcgc ccacttgggc tctgccccca ttgctgccat atcactgaca
     8101 ttgaccgcca tggctttcca gccgatagcg aacggatcaa tgtcagcaaa gaaatgggtt
     8161 ccggctactg acatatccgc cgacacagcc agttggtaac cagcacgcac gcggatgagt
     8221 gctgcatcat cgccgatgcc tacatctgcc tgcgctggcc gcgtgaaata ctttgcgata
     8281 atgtcaaatt cagccacgtc gcccattcca ccatcgctgc cacgcaatca ccacacccca
     8341 ggccaataaa atacccacca catcagccac ccagtcaaat acgctcggct ggcgagtcgt
     8401 cgttaacaca ccttgcagga cttccatcaa ggcaccaaaa acggttagac ccagcatgac
     8461 acttttaaaa tagcgctgaa aagccagcaa tccccaaaaa gccacgccgc caaaggcaac
     8521 gatatgttgc accttgtctt tataaagtga cccatcgtta gtgggagcca tttccatcag
     8581 gagtaaatat gtcagtacag ccaggcaaag gataaaaatc agcttgtaag tggttttgta
     8641 aaacatgtca ttcctaattc gatgcgcggg tagcaggtgg gcgtattgca gattttggac
     8701 gaaacgactt caccacagtc tcgtccgttt ccaggtaagg catgggccga tcaggcgtcg
     8761 ctagcagatc aagacaataa ggaacggcag caaatattcc cggggctact gacttgccct
     8821 cctcatctct caaaccttcc agtgtttcct gaatagactt gggttgcccc ggcaaattga
     8881 tgatcaggca ctgcttacga attacggcga cctggcgcga caaaatggcg gtagggacaa
     8941 aacgcaggct aatttgccgc atttgctccc caaagcctgg catttcacgg tcagcaacag
     9001 ccaatgtcgc ttcaggcgtc acatccctga gtgcgggacc ggtgccgcct gtggtcaaga
     9061 tcaggtgaca ggcctcttca tcaaccaatt gttccagcgt cctggaaata gtaccttgat
     9121 catcggcaat caaacgggtt gccaaggtat acggtgaaat cagtgcttgt gctagccagg
     9181 cagtcagggc aggaataccc ttatcttcat aaatgccttg tgaggcgcgg tcgctgatcg
     9241 aaattaagcc gatgcgcgtg atttgttctt gcataaccta gaggatgggg tctgtgttaa
     9301 tgtatgagga catcataaca ttacaacctg ttattcgatc atggagaacc ctatgaatat
     9361 accgtacaac catgggcagc tggccacatc agcaagcgtg cggagaaatc cattaccggc
     9421 accatccttg ctatggctaa cgattttact cgtggcagcg accctgctgg ccagccctgc
     9481 aacacgagca gaagacaatc cttacgccag caattatcag gcacagaacc agggcaatct
     9541 acactccatg caggcaaatc ccgagcctca actattcagc ggcacccgga gagacgaaga
     9601 caatatcaaa atgctggaaa acggctatga cctcatgggg ttcagcagct ttgaagctga
     9661 agatgttcca actgagcagg tcattagcca tggacgcact attcgggctg atagcatcct
     9721 ggtctatgtc aaaaaagctg gcaatacgac accagcctct aaaattgaaa tgatcaagga
     9781 agcaagcagg aaaggtatag ccctaacaga aaaagacatg gcggtcgatc cgacaaaata
     9841 tcgctattac gctacttatt gggcgaagtt accgccgccc gtgttagggg tacacgtgat
     9901 caaactggtg cctcgctcca gcaatgaaaa agaaggcccc tctgtgagca gcgatggtgt
     9961 acgcgtgatc gccgtcattc acggttcagc cgctgaaaaa gcaggcttat tacgtggtga
    10021 ccagctactc agcattaatc aggaaaaagt gcaggatgcc gccgaacttt ctactctggt
    10081 acgcaaatat cgtggaaaac gcgttcagtt acaattagaa cggcaagatg aaccaataca
    10141 gctagaggcg cagttataaa acatcatggc atgcatgcta ttcttgctct aaccagtatc
    10201 aaagaaagtg tttcacatgt ttcaacccat ttttgatcac cagaccgctc gtgaagcggc
    10261 tggagatatc aatgatattg aatttatacg cgccctgttt gataacatcc ggcctaaaga
    10321 atccaccgat attgaaagcg caagcaatgc cttacaagca ttatgcttct tactgcagca
    10381 gcaacctcag tttgccatgc tgctgcgtaa cagcctgtta cgactgctca atgaaaaaac
    10441 ggtgatctcg ctttactc
//
LOCUS       DATMSK010000065         6834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_123324,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000065 DATMSK010000000
VERSION     DATMSK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123324"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VNZ84_02140"
     CDS             complement(<1..357)
                     /locus_tag="VNZ84_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol oxidation system protein MoxJ"
                     /protein_id="HWU65779.1"
                     /translation="MFPDVKRVRAGWVSACLTLAFSLLTFPALAANALNVCAGKAELP
                     FSNDKQEGFENKIARVIGKALNMPINYFWWTNPLTLQKDMEAGKCDVLIGTDPNDPRV
                     LATKPYYRSAYVFITRK"
     gene            complement(456..2252)
                     /locus_tag="VNZ84_02145"
     CDS             complement(456..2252)
                     /locus_tag="VNZ84_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HWU65780.1"
                     /translation="MKLKSSRAVVGALVGGLFAGISGLAMADADLDKAVNTPGAWPIA
                     TGGYYSQHNSPLAQINKSNVKNVKAAWSFSTGVLNGHEGAPLVIGDMMYIHSAFPNNT
                     YALNLNDPGKIVWQHKPKQDASTKAVMCCDVVDRGLAYGAGQIVKKQANGHLLALDAK
                     TGKINWEVEVCDPKVGQTLTQAPFVAKDTVLMGCSGAELGVRGAVNAYDLKTGELKWR
                     AFATGSDESIRLAKDFNKDNPHYGQFGLGLKTWEGDAWKIGGGTNWGWYAYDPKLNLF
                     YYGSGNPAPWNETMRPGDNKWTMTIWGRDLDTGMAKWGYQKTPHDEWDFAGVNQMVLT
                     DQPVNGKMTPLLSHIDRNGILYTLNRENGNLIVAEKVDPAVNVFKKVDLKSGTPVRDP
                     EFATRMDHKGTNICPSAMGFHNQGVDSYDPESRTLYAGLNHICMDWEPFMLPYRAGQF
                     FVGATLAMYPGPNGPTKKEMGQIRAFDLTTGKAKWTKWEKFAAWGGTLYTKGGLVWYA
                     TLDGYLKALDNKDGKELWNFKMPSGGIGSPMTYSFKGKQYVGSMYGVGGWPGVGLVFD
                     LTDPSAGLGAVGAFKELQNHTQMGGGLMVFSL"
     gene            2448..3920
                     /locus_tag="VNZ84_02150"
     CDS             2448..3920
                     /locus_tag="VNZ84_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWU65781.1"
                     /translation="MWSIVAQVYAVQECAPAAGNFKAFGHSVMHSYQQSMPVITAIHK
                     LGRLSRPSRDTLIVITGVVLTFALSSAFDLAEHVNAWLLTYEYAQLDEVPLTLFVFVL
                     LSVWFSQRRWREIHAEIKRRQLTEAQLSSSQQLYKTLFDGDLTGNVVLDVDGNIGMHN
                     RAFDRICRPLSQASNARDLFSFDWSTFVLQLVHHHEINFSKLQVHRPDGLPCYVGARF
                     VHVLAADQQPAQIHGYLVDMTEQCLVELDLERTLKENRVLARHAMQMQEQERKYIASE
                     IHDETGQYLTAIRMDALTLQKSDPDQTHVIAVRIASNTQHVQQSIRALIKHLRPPALD
                     SLGLLGAVERLVNDWKKLNPQVECHLELDTGEQALNEDINIVAFRVVQEALTNISRHA
                     HASEVWIDIQVLKRQDKSMLQIEIRDNGKGMDTSQSTEGVGLVGMRERIESLQGSFKL
                     MSGLQAGTLITGLIPLPGQRQPLPLSASTSTAGVNDYATP"
     gene            3907..4260
                     /locus_tag="VNZ84_02155"
     CDS             3907..4260
                     /locus_tag="VNZ84_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65782.1"
                     /translation="MQHRKWLALAAFIFPLSLSAKDLQTEERPFLDAYSGKSRQYVEE
                     NLGKPVKKEVAVKPHNADQILQEKGQATAGEGDSVEMWYYRGKIQYAPNKFFNTAELT
                     FANDKCVNITFANKK"
     gene            4273..4905
                     /locus_tag="VNZ84_02160"
     CDS             4273..4905
                     /locus_tag="VNZ84_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU65783.1"
                     /translation="MAKVMIADDHAMLRQGLRTMLEQAHHEVTTDALSGEEACLLVEK
                     NHPELLILDLDMPGIGGLETLKRILHRKPQMRVLIFSMHDDCIYATRAIQAGARGYVT
                     KTEPPEVVLEAVQKILKGGRYINNELAQSLSMYHLESQENPIQKLSPREFEVFRRIAQ
                     GEALAAIAKGMHIGYKTVANIQTSVRQKLGVETTGQLVHIAVRFGIIKGL"
     gene            4920..5666
                     /locus_tag="VNZ84_02165"
     CDS             4920..5666
                     /locus_tag="VNZ84_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU65784.1"
                     /translation="MITIIARKELRSLFATPMGWLVLAIFQAIVGTYYSLSFNQYFEI
                     MHSAQWQVQRVGITQFMAEGVFGVAAVLMLFVVPLVTMKLISEERKNQTIALLMSAPL
                     SMSELILGKLLGIVSYLSLLIVTMVVMIAMLLPWAEIDLPYLLTHAFGLWLLMVASSA
                     LGLYVSCLTANPILAAFCSLTTLTLWLMLDKFFSDSSHAWFHDFSLMQHYRQFALGVL
                     NSYDILFFVLFALFFVLLAIRRLHADRLYG"
     gene            5659..>6834
                     /locus_tag="VNZ84_02170"
     CDS             5659..>6834
                     /locus_tag="VNZ84_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HWU65785.1"
                     /translation="MANSGRSQTTQRWLAVLTTWWQHRLAVPALLVLTIALGVLAWQF
                     NLVADTTHNHRNTLSAASKQVLQQLPGRIEVTAFCSNSPYKGRYFRKSITALVQRYQH
                     LHVGLDLQFIDPAYAPTLAREQNIKKEGEMIIRYRGQEKRMYLPYTEEAFTNVLLQLQ
                     HGERAPILFASGHGEPALDNTTAHGASHLAFALREAGLQVMQSDTLEFSQRSKRPTLI
                     LAGATQPYTVMEAKAIQAHIDQGGNLVWLTDSASTQGLQTIADSLGLDISQGLVIDPP
                     NRQFDIPLHALSTQRYSNQGPTQDFALRTFFDSAHAIRRPRQYSDTWHVFPLVAAAEH
                     GWVSHAYRSEHPGTIPAFNPEADIQGPATIALALEKARPSGELQRILVIGSRQFFKND
                     "
BASE COUNT         1659 a         1838 c         1691 g         1646 t
ORIGIN      
        1 tttgcgtgtg atgaacacat aggctgaccg gtaataaggc ttggtcgcta ggacgcgtgg
       61 atcattagga tcagtgccaa tcaacacgtc acatttacct gcttccatgt ctttttgcag
      121 ggtgagcgga ttggtccacc aaaaatagtt gattggcata ttcaacgcct tgccaatcac
      181 gcgtgcaatc ttgttttcga agccttcttg cttgtcattt gaaaaaggca actcggcttt
      241 accagcacag acattgagtg cattagcagc caaggcaggg aatgtcagca ggctgaatgc
      301 caacgtgagg caggcactta cccagccagc acggactctt tttacatcag gaaacatatt
      361 tgctctcttc actctagggg ctgagaacag ggtggccgcc cgtcttgcga ttgatggcca
      421 ccctgcacac tacatcaaca ggttcagtac cttaattaca ggctgaacac catcaggcca
      481 ccgcccattt gtgtgtggtt ttgcagttct ttgaacgcac caaccgcacc caaaccagca
      541 cttgggtctg tcaggtcaaa gaccaaacca acaccaggcc agccgccaac accgtacatg
      601 ctgccgacgt attgtttacc cttgaaggag taagtcatag gagatccgat accaccagat
      661 ggcatcttga agttccacag ctctttaccg tctttgttat ccaaggcttt caggtaaccg
      721 tccagtgttg cgtaccagac caggccgcct ttggtgtaca aagtaccgcc ccaagctgcg
      781 aatttctccc acttggtcca tttagcctta ccagtggtca ggtcaaatgc acggatttgg
      841 cccatttctt tcttggttgg gccgttaggg ccagggtaca tcgccagggt tgcaccgacg
      901 aagaactgac ctgcacggta tggcagcatg aacggttccc agtccataca aatgtggttc
      961 aggcctgcgt acaaagtacg gctttctggg tcgtaagagt caacaccctg gttgtggaag
     1021 cccatcgctg atggacaaat gttggtgcct ttatggtcca tacgggttgc aaactctgga
     1081 tcacgtactg gtgtacctga tttcaagtca acctttttga acacattcac ggctggatcc
     1141 actttttcag caacgatcag gttgccgttt tcgcggttca gtgtgtacaa gataccgtta
     1201 cggtcaatgt ggctcaacaa tggtgtcatt ttgccgttga ctggctgatc agtcagcacc
     1261 atctggttca cgccagcgaa gtcccactcg tcatgaggtg tcttttggta gccccatttc
     1321 gccatgcctg tatccaggtc acgaccccag atcgtcatcg tccatttgtt gtcgccagga
     1381 cgcatggttt cattccatgg cgccgggtta cctgaaccgt agtagaacag gttcaattta
     1441 ggatcgtatg cataccaacc ccagtttgta ccgccaccga ttttccaggc atcgccttcc
     1501 caggttttca ggcccaggcc gaactggccg tagtgtggat tgtctttgtt gaagtcttta
     1561 gccagacgga tagactcgtc agaacctgtg gcgaaagcac gccatttcag ttcaccggtt
     1621 ttcaggtcgt aggcgtttac tgcaccgcgc acacccagct cagcacctga gcaacccatc
     1681 aacaccgtgt ctttagccac gaatggtgct tgtgtcagtg tttgacccac tttaggatca
     1741 catacttcca cttcccaatt gatcttgccg gttttagcat ccaacgccaa caagtgaccg
     1801 ttagcttgtt ttttcacgat ttgacccgca ccgtatgcca gaccacggtc aaccacgtca
     1861 cagcacatca ccgctttggt ggaagcatct tgcttgggtt tgtgttgcca aacaattttg
     1921 cctggatcgt tcaggttcaa ggcgtatgta ttgtttggga aagcagagtg gatgtacatc
     1981 atgtcaccga tcaccaatgg cgcaccttcg tgaccattca gtacaccggt ggaaaatgac
     2041 cacgcagcct tcacgttttt aacgttagat ttgttgattt gcgccaatgg gctgttgtgc
     2101 tggctgtaat agccaccagt cgcgattggc caggcgccag gtgtattcac cgccttgtct
     2161 aggtctgcat ctgccatggc caggccggaa atgcctgcga acaagcctcc aaccaaagcg
     2221 ccgacgacgg cacgggatga tttcagtttc atactttctc ctaatctctt aagaatgcgt
     2281 tttttaagtg ttgttgcaga cacccccccg aatgccgcac ttcacgaacc gaattgttaa
     2341 taatttgtaa caaagacaat agatgaatga ttccctatga tgaattcaag aattcattca
     2401 catggactgg ccaataaagg gaatttttca cggggcatcc agagaggatg tggtctatag
     2461 tggcgcaggt ttatgcagtg caggagtgcg ctcctgctgc aggcaatttc aaagcgtttg
     2521 gtcattcagt catgcattct tatcaacagt ctatgccagt cattactgct attcacaaat
     2581 taggcaggct tagccgcccc agccgggaca cgctgatcgt tatcacgggc gtggtattga
     2641 cctttgcctt gtcctcggca tttgatctgg ccgaacatgt gaatgcctgg ttactcacct
     2701 atgaatatgc acaactggat gaggtcccgt tgaccctgtt tgtttttgtg cttttgagcg
     2761 tgtggttcag ccaacgccga tggcgggaga tacacgcgga gattaagcgc cgccaactca
     2821 cagaggcaca attgagcagc agccagcagc tctataaaac cttgtttgat ggtgacctga
     2881 cgggcaatgt cgtgctggat gtggacggca atatcggcat gcataaccgc gcctttgatc
     2941 gcatttgccg cccactcagc caagcgtcca atgcacgcga cctgttctcg tttgactggt
     3001 caaccttcgt tttgcagtta gtccatcacc atgaaatcaa tttcagtaaa ttgcaggtcc
     3061 atcgcccgga cgggctaccc tgttacgtgg gggcgcgctt tgtgcatgtg ctggccgctg
     3121 accagcaacc ggcacaaatc cacggctacc tggtagatat gacagaacaa tgcctggtcg
     3181 aactggacct ggaacgcacg ttgaaggaaa accgcgtgct ggcacgccat gcaatgcaga
     3241 tgcaagaaca ggaacgcaaa tatattgcca gcgagattca tgatgaaacc ggccaatatt
     3301 taaccgccat ccgcatggat gccctgacat tgcaaaaaag cgatccggat caaacacatg
     3361 tgattgccgt caggatcgcc tccaataccc agcatgtaca acaatctatc cgcgccctga
     3421 tcaaacattt gcgcccacca gcactggact ctctggggct gctcggtgca gtagagcgcc
     3481 tggtaaacga ctggaagaaa ttgaatccgc aggttgaatg tcatcttgag ctagataccg
     3541 gcgaacaggc attgaatgaa gatattaata ttgtggcctt tcgcgtggtg caagaagccc
     3601 tgaccaatat ttcacgtcat gcccatgcca gtgaagtgtg gatagacatc caggtgctca
     3661 aacgccagga caagtccatg ctacaaattg agatccgtga caacggtaaa ggcatggata
     3721 cctcgcaatc cacggagggc gtgggcctgg tcggcatgcg cgagcgtatc gaatcactgc
     3781 aaggcagttt taaacttatg agcggactac aagctggtac tttgattacc gggttaattc
     3841 cattacctgg gcaacgccaa cccttaccgc tttcagcttc aacctcaacc gcaggagtca
     3901 atgactatgc aacaccgtaa atggctggct ttagccgcct tcattttccc attgtccctt
     3961 tccgccaaag acttgcagac agaagaacgt ccgttcctcg atgcctatag cggtaaatca
     4021 cgtcaatatg tggaagaaaa cttgggcaag ccggtgaaaa aagaagtcgc cgtcaaacca
     4081 cacaatgctg accagattct gcaggaaaaa ggtcaagcaa ctgcaggcga aggtgacagt
     4141 gttgaaatgt ggtattaccg cggcaaaatt caatacgcac cgaataagtt tttcaacacc
     4201 gctgaactca catttgccaa tgacaaatgt gtgaatatca cctttgccaa caagaaatag
     4261 agtagaccgt tcatggcaaa agtaatgatt gcagatgatc atgccatgct caggcaagga
     4321 ttgcgcacca tgcttgagca ggcacaccat gaagtgacca cagacgcact gagtggcgaa
     4381 gaagcctgtt tactggtgga gaaaaatcat ccagaactgc tgatcctcga tctggatatg
     4441 ccgggcattg gtgggctgga aaccctgaaa cgcatcctgc accgcaaacc gcaaatgcgt
     4501 gtattgatat ttagcatgca cgatgactgc atttatgcca cccgtgccat ccaggcgggt
     4561 gcacgcggct atgtcaccaa aaccgagccg cctgaggtgg tgctggaggc ggtacaaaaa
     4621 atactcaagg gtgggcgtta catcaataat gaactggcgc aaagcctgtc catgtaccat
     4681 ttggaaagcc aggaaaaccc gatccagaaa ctctctccac gagagtttga ggtgttccgg
     4741 cggatcgcgc agggagaggc gctggcagcg attgcgaaag gcatgcatat tggctacaaa
     4801 accgtggcca atatccagac cagcgtgcgc caaaaactgg gcgtggaaac gactggccag
     4861 ctggtccata ttgctgtccg ctttggcatc atcaaaggac tctgacggcg agcgttgcca
     4921 tgatcaccat catcgcccgc aaagagttac gcagcctatt tgccaccccc atgggctggc
     4981 tggtgctcgc cattttccag gcgattgttg gcacctacta tagcctgagt ttcaaccagt
     5041 actttgaaat catgcacagt gcccaatggc aggtgcagcg cgtaggcatc acccagttca
     5101 tggcggaagg cgtctttggg gtggcagccg tactgatgct gtttgtggtg cccttagtca
     5161 ccatgaaact gatcagcgaa gagcgcaaga atcagaccat cgccttactc atgagcgccc
     5221 ccctcagcat gagcgagctg atcctgggca aactgctggg gattgtcagc tacctaagcc
     5281 tgcttattgt caccatggtc gtcatgattg ccatgctatt accctgggcc gaaattgacc
     5341 tgccatattt gctgacgcac gcctttggct tatggttgct catggtcgcc agcagtgcgc
     5401 ttgggcttta tgtctcctgc ctgacggcca acccgatcct ggccgctttt tgcagcctca
     5461 ccaccctcac gctatggctc atgctggaca agtttttcag cgacagttcg catgcctggt
     5521 ttcatgactt ctccctgatg caacattacc ggcagtttgc ccttggcgta ctcaacagct
     5581 atgacatcct gttttttgta ctgtttgcac tgttttttgt actgctggcc attcgccgtt
     5641 tacatgcaga ccgactctat ggctaattcc ggacgttctc aaaccactca acgctggctg
     5701 gcagtcttaa cgacatggtg gcaacatcgc ctggcggtcc cggccttgct agtgctcacc
     5761 attgccctcg gtgtgcttgc ctggcagttc aacctcgtgg cggataccac gcataaccac
     5821 cgcaacacct tgtctgctgc cagcaaacag gtattgcaac aattgccagg tcgtatagaa
     5881 gtgacggcat tttgcagcaa cagcccttac aagggccgct atttcaggaa gtcgatcacc
     5941 gcattggtac agcgctacca gcatttacat gtcgggctgg atttacagtt tatagaccct
     6001 gcctatgccc ccacgctggc acgcgagcag aatatcaaga aagagggcga aatgattatc
     6061 cgctatcgcg gacaagaaaa gcgcatgtac ctgccttaca cagaagaggc attcacaaac
     6121 gtactgctac agctgcaaca tggtgagcgg gcgcccatcc tatttgccag cgggcatggc
     6181 gaacctgcct tggacaatac aacggcacac ggagccagcc atcttgcctt cgcactgcgt
     6241 gaggcaggcc tacaggtcat gcaaagtgat acgcttgaat ttagccagag aagcaaacgg
     6301 ccaacgttga tacttgcagg agcgacccaa ccctacaccg tcatggaagc gaaagccatc
     6361 caggcccata ttgaccaggg tggcaacctg gtgtggctga cagattcagc atctacacag
     6421 ggcctgcaaa caatcgctga cagcctgggg ctggacatta gccaggggct ggtgattgac
     6481 ccgcctaatc ggcaatttga tattccatta catgcgctca gtacccagcg ctactccaac
     6541 cagggaccga cacaggactt tgccctgcgt actttttttg acagtgcgca cgccatccgc
     6601 cgcccgcggc aatacagtga cacttggcat gtgtttccgc tggtcgctgc cgctgaacat
     6661 ggttgggtca gtcatgctta ccgcagcgaa catccaggga caataccggc attcaatccc
     6721 gaagcagata ttcaaggccc cgccaccatt gcgttagcct tggagaaagc acgcccttcc
     6781 ggcgaattac aaagaatttt ggtaattggg agtcgccagt tttttaaaaa tgac
//
LOCUS       DATMSK010000066         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_452430,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000066 DATMSK010000000
VERSION     DATMSK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452430"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /gene="glnA"
                     /locus_tag="VNZ84_02175"
     CDS             complement(<1..1379)
                     /gene="glnA"
                     /locus_tag="VNZ84_02175"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HWU65786.1"
                     /translation="MSVANVMKLVAENDIKFVDFRFTDTRGKEQHVTVPVSAFDEDKF
                     TEGHAFDGSSIAGWKGIQASDMQLMPDASTAYIDPFFDEPTLVLTCDVVDPTDGKGYD
                     RDPRSLAKRAEAYLKSSGLGDTAYFGPEPEFFIFDSVQWDATMGGSFVKINSEEAAWS
                     SGEKFEGGNTGHRPAVKGGYFPVPPVDSLHDIRSAMVMTLEELGVPVEVHHHEVATAG
                     QCEIGTKFATLTQRADWTQILKYVVTNTAHAYGKTATFMPKPMFGDNGSGMHVHQSVW
                     KDGKNLFAGNGYAGLSDFALYYIGGIIKHARALNAITNPGTNSYKRLVPHYEAPVKLA
                     YSAKNRSAAIRIPFVHSDKARRVEARFPDPIANPYLAFSALLMAGLDGVQNKIHPGEP
                     ATKDLYHLPPEEDALIPTVCSSLEQALEYLDKDREFLTRGGVFTDDWIDSYIALKMEE
                     VTKLRQTPHP"
     gene            1731..2180
                     /locus_tag="VNZ84_02180"
     CDS             1731..2180
                     /locus_tag="VNZ84_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWU65787.1"
                     /translation="MESIEKILSQAHTRAEQTGLPYAGAVTPAEAYALISQDADAVLV
                     DVRSRAELELVGRVPVATHVEWALYPGMVANTEFAAQLLAQVDKSRTVVFMCRTGGRS
                     HNAALLAQQLGYSKAYNMVEGFEGEANELKQRTLINGWKHAGLPWTN"
     gene            2193..3017
                     /gene="aroE"
                     /locus_tag="VNZ84_02185"
     CDS             2193..3017
                     /gene="aroE"
                     /locus_tag="VNZ84_02185"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWU65788.1"
                     /translation="MAIEKYAVIGHPIEHSKSPLIHQAFAQQFGKTIQYEKVLAPLDG
                     FLATVKRLQVEGYLGANVTVPFKFEAFDASQILAARAQAAGAVNTLSFRDQCVTGDNT
                     DGCGLVNDILKHQNASLAGQKVLLLGAGGAAQGVMQPVLEQKPLQLTVANRSVDKAQT
                     MVERFAADAKRSETSLLVQSFSELSQHYDVVINATSAGLTESNLQLPAVIFQSNTLAY
                     DMMYGRETPFMQQARAAGAQVADGLGMLVEQAAEAFYLWHGVRPDTAPVIKSFRQS"
BASE COUNT          795 a          701 c          840 g          812 t
ORIGIN      
        1 gggtgaggtg tttgacgcaa cttggtgact tcttccattt tcaatgcaat gtaagaatca
       61 atccagtcat cagtgaaaac accaccgcgt gtcaggaact cgcggtcttt gtccagatac
      121 tcaagcgctt gttctaatga agaacatacg gttgggatca atgcatcttc ttctggcggc
      181 agatggtaca agtctttagt cgctggttca cctggatgaa tcttgttctg tacgccatcc
      241 aagcccgcca ttaacaaggc agagaatgcc agatatgggt tggcaatcgg atctgggaaa
      301 cgcgcttcaa cgcggcgtgc tttgtctgaa tgtacaaacg gaatacgaat cgcagcagaa
      361 cggttcttag cagagtaagc cagttttact ggtgcttcgt agtgaggtac caaacgcttg
      421 taagagttag tgcctgggtt ggtaatcgcg ttcaaggcac gtgcgtgctt gatgataccg
      481 ccgatgtagt acagcgcaaa gtcgctcagg ccagcatagc cgttacctgc aaacaggttt
      541 ttgccatctt tccatacaga ttggtgtacg tgcataccgg aaccattgtc accaaacatt
      601 ggtttaggca taaatgtggc agttttaccg taagcgtgtg cggtgttggt taccacgtat
      661 ttcaggattt gcgtccagtc agcacgctga gtcagtgttg caaatttagt accgatttca
      721 cattggccgg cagtggctac ttcatggtga tgcacttcaa caggtacacc caactcttcc
      781 agagtcatga ccatggctga acggatatca tgcagtgaat cgactggtgg cactgggaag
      841 taaccgcctt ttaccgctgg acggtggcca gtgttgccgc cttcaaattt ttcgccagat
      901 gaccaggcag cttcttcaga gttgatctta acaaagctac cacccatggt ggcatcccat
      961 tgtacgctgt caaaaatgaa gaactctggt tctgggccaa agtaagctgt gtcacccagg
     1021 ccgctggatt tcaaataagc ttctgcacgt ttagccaagc tacgtgggtc gcggtcgtaa
     1081 cctttaccat ctgtagggtc aaccacatca caggttaaaa ccaatgttgg ctcgtcaaaa
     1141 aacgggtcaa tgtatgcggt gcttgcatca ggcatcagtt gcatgtcaga ggcttgaatg
     1201 cctttccaac cagcgatgga agagccatcg aaggcgtgac cttcggtaaa tttatcttcg
     1261 tcgaaggcag acactggcac ggtcacgtgc tgttctttac ctctggtgtc ggtaaaacgg
     1321 aaatcaacaa atttgatgtc gttttctgca accaatttca ttacgtttgc aacggacatt
     1381 acattctcct cagtgatcaa ggtctaaaat gattaagttt gcgcacgaaa aaacggggca
     1441 tgttgcggat gtcccgaaac gtgagcaatg gtaaacgatg gataattggg gatcttgatt
     1501 ggtgcgcgat cccacttaat cttagtatgt aatattagca ggaagcatgc cacccaaaaa
     1561 tacgcatttt gcattgtagc ggggtatctt gcataaattt gtaaaatccg tgcgcaccgt
     1621 taatgtgcaa tgcaacaaat tgagcaccaa agtggtgcaa caagattttg tatggctttc
     1681 acaggtatac tcgccatttt agtatgagtc gttcggaacg aaggtgtaat ttggaaagta
     1741 tagaaaaaat cttgagtcag gcgcacacgc gtgccgagca aactggtttg ccgtatgccg
     1801 gagcagtgac gccggcagaa gcgtacgcct tgattagcca ggacgctgat gccgtgttag
     1861 tggatgtgcg ttcccgtgcc gagctagagt tagtggggag agtgccggtg gccacgcatg
     1921 tcgagtgggc actttatcct gggatggtgg caaataccga gtttgctgca caattgttgg
     1981 cacaggtaga taaatcacgc accgtggtgt ttatgtgccg cacgggtggc cgcagtcata
     2041 acgccgcttt gctggcacag cagttgggtt atagcaaagc atacaacatg gtggaagggt
     2101 ttgagggcga agcgaatgag ctgaagcagc gtactttaat taacggctgg aagcatgccg
     2161 gattgccctg gacaaactaa acttcacaac acatggctat tgaaaaatat gcagtgattg
     2221 gtcatccaat cgaacatagt aagtcgccac tgattcatca ggcgttcgcg cagcagtttg
     2281 gtaaaacgat tcagtatgaa aaagtattag cgccactgga tggctttctt gcaacggtga
     2341 agcgattgca agtcgaaggt tacttgggtg ccaatgtcac tgtgccgttc aagtttgagg
     2401 cgttcgatgc cagccagata ttggctgcac gtgcgcaggc ggctggcgcg gtaaacacct
     2461 taagttttcg tgatcaatgt gtgacgggtg acaacaccga tggttgtgga ctcgtgaacg
     2521 acattctcaa gcaccagaat gcctcgctgg ccggccaaaa ggtattgtta ctgggagctg
     2581 gcggcgcagc gcaaggcgtg atgcaacctg tgctggagca aaagccgttg cagttgacgg
     2641 tagccaaccg tagcgtggat aaggcacaaa ccatggttga gcgatttgcc gcagatgcaa
     2701 aacgcagcga gacatcgctg ctggtgcaat cattttcaga gttatcacag cactatgatg
     2761 tggtgatcaa tgcgacctct gcgggattaa ccgagagtaa tttgcagctt cctgcggtga
     2821 tatttcagtc aaacacactc gcctatgaca tgatgtatgg ccgtgaaaca ccgtttatgc
     2881 aacaagcgcg ggctgcaggt gcgcaagtgg ccgatggctt gggcatgttg gtagaacaag
     2941 ccgctgaggc attttatctt tggcatggtg tacggcctga caccgcgcca gtgatcaagt
     3001 cttttcgcca gtcttgagtt gagcccatga aacaagccat taaactgttt tttgggctgc
     3061 tactgctgtg ggtatttctt tatcaagtct gggtgttatt acatattttg tggtggatag
     3121 aacataaccc aacatccaca gcttttat
//
LOCUS       DATMSK010000067        10782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_217106,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000067 DATMSK010000000
VERSION     DATMSK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10782
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217106"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VNZ84_02190"
     CDS             complement(<1..1100)
                     /locus_tag="VNZ84_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWU65789.1"
                     /translation="MADDENTITLSPVVVTATRQAQNSFDLPVAIDVVTEKDIKQGQL
                     QMQLSESLIRVPGLTAQSRNQQAQDPQISSRGFGSRSSFGVRGIRLYVDGIPLTMPDG
                     QGQPGVVDLSSIKSIEVMRGPFSSLYGNSSGGVIQMFTKDAPTTPTIDATTMFGSYDT
                     KRNVLEAAGRSEEVEYMLNVSNFESAGYRDHSSSNKKMGTAQFKFHISPDTKLTTLVN
                     WFEQDAQDPLGLTRADIATDRKGVVAAANNANTRVSRNHTQVGFNLEHAINETNKLSF
                     VPYVGTRNNSQILPFNATGTNARTSKIARDFYGTDLHWDNNGEILGKKYNLTFGINYG
                     KSEDNRLDVNTQIAGAPANVTNRDEVNISTNY"
     gene            complement(1276..2466)
                     /gene="coaBC"
                     /locus_tag="VNZ84_02195"
     CDS             complement(1276..2466)
                     /gene="coaBC"
                     /locus_tag="VNZ84_02195"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWU65790.1"
                     /translation="MTKTHASSLHLLLGITGGIAAYKTAELVRLLIKQGHSVQVVMTE
                     AATHFITPTTLQALSGNPVFVDAWDSRIANGMPHIELSRKADAILVAPASADFLAKLA
                     HGFANDLLSTLCLARECPLLVAPAMNRQMWENAATQRNIAQLNQDGITVLGPESGEQA
                     CGEVGQGRMLEPEALLDDLQAFFTPKLLASKKVLITAGATMEMLDPVRGITNLSSGKM
                     GYALAQVAMRMGAEVTLVYGHAQVPRPVVSHAYYAGSANEMYQTVMQHIADQHIFIGV
                     AAVADFRPESSSIQKIKKSEESLTLKLIKNKDILADVASLPNPPYCVGFAAETEQVLE
                     HARQKRLAKRVPMIVANLANTAMGSDQNTVTIIDAKGEHAFETDTKEKIAFKLLSHIN
                     SLVT"
     gene            2567..3334
                     /locus_tag="VNZ84_02200"
     CDS             2567..3334
                     /locus_tag="VNZ84_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65791.1"
                     /translation="MIKLVRIFFWGVVVLTALMQLWQEYQTLQWRKPLNVALYPINAD
                     DAPQVDTYIANLRTKDFDVISQFFEREALKYHLPLQYPVHLYLGPTIQDVPSAPPAVN
                     GHLLDIILWSLKFRWFSWRHQPPVGVPIDIKLYLLYHDPARHKHLLHSTALHKGRIGR
                     VNLFADHRQHAVNTVIIAHELLHTLSASDKYDLNANLPLFPQGYAEPYLQPVYPQQLA
                     EVMAGKVPITAHRARMAKGLEETIIGRPTAREIGWVK"
     gene            3353..4027
                     /gene="radC"
                     /locus_tag="VNZ84_02205"
     CDS             3353..4027
                     /gene="radC"
                     /locus_tag="VNZ84_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWU65792.1"
                     /translation="MAITDWPEQERPREKLLFAGATALSDAELLAIFLRVGVPGKSAV
                     DLARDLLHHFKSLNGIFSASLRDVQVVHGMGVSKYCQLQAVLEMSKRALGESLQQTDS
                     FCSPTQVKHYLQLQLSSLQREVFGIMFLDAQNRLITYETLFQGSLMQTSVYPREVVKR
                     ALALNAAALILSHNHPGGSAKPSLADEQLTQSLKDALSLVDIKVLDHIVVSGQATFSF
                     SEMGLI"
     gene            complement(4045..4120)
                     /locus_tag="VNZ84_02210"
     tRNA            complement(4045..4120)
                     /locus_tag="VNZ84_02210"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4085..4087),aa:Val,seq:tac)
     gene            complement(4212..4445)
                     /locus_tag="VNZ84_02215"
     CDS             complement(4212..4445)
                     /locus_tag="VNZ84_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HWU65793.1"
                     /translation="MRPLSPDTTASLSSTPRILLSPEDQKLVDSAKDILMHQRDLSEE
                     QAYSLLSEMADKRKTGMVDMSLQLINITKRLTV"
     gene            4702..6753
                     /locus_tag="VNZ84_02220"
     CDS             4702..6753
                     /locus_tag="VNZ84_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HWU65794.1"
                     /translation="MKAFWSRAGWASLVLLGAAAFSNVALARGESLNALWLITAAVCI
                     YLIAYRFYAAWIAANVLAINATRATPAERLNNGRDYVPTNRWVVFGHHFAAIAGPGPL
                     VGPTLAAQFGYLPGTLWILIGAVLGGAVQDMVTLFLSTRRNGRSLGQMARDEIGAIGG
                     TAALIGTFIIMIILIAVLGLVVVNAMKHSPWGTSTVAATIPIAMLVGVYMRYLRPGRV
                     LEASLLGVALLLASVVFGGWVDHHPQLATFFDHEGLPLAFSVIAYGFAAAVLPVWLLL
                     APRDYLSTFMKLGTVIMLAIAILWLHPEIHMPAITPFIDGSGPIFGGKLFPFVFITIA
                     CGAISGFHALIASGTTPKLITSENDIRVIGYGAMLLESFVAVMAMVAATVLEPGVYFA
                     INSPAGVVGKEALDAVNTISSWGYQVTVEQMQHLASAMGESTLFARTGGAPSLAVGMA
                     SILGNVFGEHMLALWYHFAIMFEAIFILTTLDAGTRVGRFMLQDMLGNLHPKLGETSY
                     TPSVILTSALVVAGWGYFLYIGVIDPNGGVNILWPLFGIANQMLAAIALSVATGILIK
                     SGKASKAWITGLPLLWLLTITTTAVYEKVFSDDIRVGFFAAANDLAQKLSFGVLPPEK
                     AAVAPQLIFNQQLDAWLTIFFVLMLWIVVLDMLRMSWRSLAGKVVLSSSEAPYVASRL
                     G"
     gene            6765..6950
                     /locus_tag="VNZ84_02225"
     CDS             6765..6950
                     /locus_tag="VNZ84_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HWU65795.1"
                     /translation="MRKLKLFWQRVRQLSGDDAYERYLAHYNEHHAQPGAAAPLTRAD
                     FFKQWQDQKWTGIKRCC"
     gene            6923..7198
                     /locus_tag="VNZ84_02230"
     CDS             6923..7198
                     /locus_tag="VNZ84_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HWU65796.1"
                     /translation="MDRYQTLLLSEGDISRIIEMAWEDRTPFEAIEHQFGLRQADVIV
                     LMRKQLKPRVFASWRKRTRGQQTKHASLRSPNVNRHCSSFQHKQVRP"
     gene            7302..9158
                     /gene="ilvD"
                     /locus_tag="VNZ84_02235"
     CDS             7302..9158
                     /gene="ilvD"
                     /locus_tag="VNZ84_02235"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWU65797.1"
                     /translation="MPKYRSHTTTQGRNMAGARALWRATGMKDEDFSKPIIAVANSFT
                     QFVPGHVHLKDLGQLVAREIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADALVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAGKVNWQGQVR
                     KLDLVDAMVEAADSHVSDAEVAEVERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSTLATHAARKQLFLQAGRLIVDLAKRHYEQDDYTVLPRNIATMRAFENAMSLDVAMG
                     GSTNTVLHLLAAAHEAGVDFTMKDIDRISRQVPCVSKVAPATSKYHMEDVHRAGGVMG
                     ILAELNRAGVIHTDVPTVHSTSMGEALAKWDIVTTQDEEVKKFYRAAPGGIATTIAFS
                     QSMLWPELDDDRENGCIRNKEHAYSQDGGLAVLYGNIAQDGCIVKTAGVDDSILKFTG
                     RARVFESQDSAVEAILADKIVAGDVVVIRYEGPRGGPGMQEMLYPTSYLKSKGLGKAC
                     ALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVQEGDTIEIDIPNRTIHLAVSDEEMA
                     HRRSHMEAKGHDAWKPLNRERLVSPALRAYAAMSTSAAKGAVRDVAQIERRR"
     gene            9158..10093
                     /locus_tag="VNZ84_02240"
     CDS             9158..10093
                     /locus_tag="VNZ84_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HWU65798.1"
                     /translation="MNNECGILSKSMEMTKRMKLHAHVNQWQDENGLQYLEIDNPLAT
                     GKIALQGAHVMHWQPKFLAHPVLWLSSNARYVKGRSIRGGTPICWPWFGAHPTDSTLC
                     PHGFARVIPWRVMDIDATPTGATRIILEMQQTPEAQRQLSYPYELTLTITIGRRLRID
                     LATTNLAEHPFVIGEAFHTYFNVSDISNIQITGMQDLVYLDKLLKYERNVEHNALSFD
                     GQEYDRVYVDHSSDCAIHDSGYNRIIHVAKSGSDTTVVWNPGAEKAGAMGDMGVDDEW
                     RKTICVETTNALDNMVVINPGRKHTLSAEYSIETL"
     gene            10298..10621
                     /locus_tag="VNZ84_02245"
     CDS             10298..10621
                     /locus_tag="VNZ84_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWU65799.1"
                     /translation="MKVLFSAIAAATLLMAGTAHAVDAAAAKALAQKSGCLACHSVDA
                     KVLGPAYKDVAAKYKGDKGAEAKLIAKVKAGGSGVWGNIPMPANSPQVKDEDIKTIVE
                     WVLSL"
BASE COUNT         2483 a         2387 c         2961 g         2951 t
ORIGIN      
        1 tcgtagtttg tcgaaatgtt gacttcatcc cgattggtta cattggccgg tgcgcctgca
       61 atttgggtat ttacatctaa gcggttatct tcggattttc catagttaat accgaaagtc
      121 agattgtatt tctttccaag aatttcgcca ttattatccc agtgaagatc tgtcccataa
      181 aagtctctgg caatcttgct ggttctggca tttgtgcctg ttgcgttgaa tggcaaaatt
      241 tgagaattgt tccgagtccc tacataaggg acaaaagaca acttgtttgt ttcgttaata
      301 gcatgctcta gattgaagcc aacttgagta tggtttcttg atacacgggt gttggcattg
      361 ttcgctgccg caacaactcc ttttctgtct gttgcaatat ctgctcttgt gagtccgaga
      421 ggatcttggg catcttgttc aaaccagtta accagagttg tcagttttgt atctggactg
      481 atgtggaatt taaattgggc agtccccatt tttttattgc tgctgctgtg gtcgcgatag
      541 cctgcagatt caaagttgga aacattaagc atgtattcca cttcttcaga gcgccctgca
      601 gcttctagca cattgcgctt ggtatcgtaa ctgccgaaca tggtcgtagc atcgattgtt
      661 ggtgttgtgg gtgcatcttt cgtaaacatc tgaatcacgc cacctgatga gttgccgtaa
      721 agtgaagaaa acggaccgcg catgacttca atgcttttga tggatgacag gtcaacaacg
      781 ccgggctggc cttggccgtc tggcatggtc agcggtatgc cgtcaacata tagacggatg
      841 ccacgtaccc caaaagaaga tcgtgatcca aagccgcgac tcgagatttg tgggtcttgt
      901 gcttgctgat tgcggctttg agcagttagg ccgggtaccc gaatcaagct ttcggataat
      961 tgcatttgca attgtccttg tttgatgtct ttttcggtaa ccacatcaat ggcgacaggt
     1021 aagtcaaaac tgttttgtgc ctgacgggta gctgttacca caacaggaga caaggtaata
     1081 gtgttttcat catctgccat gaccaggcta gggctgctca atgccgtgga aattgcaaca
     1141 gataaggggg cgaggcgaag ggcgcgcatc tttattctca atactaaaat taaactgcgc
     1201 gattgtaagc tgtgtgtaag ctccggcgac tcagtatttt catgaaaata ctgataaaga
     1261 ttggtttatg cgcctttaag tgaccagtga gttgatgtgg ctcaaaagtt tgaaggcaat
     1321 cttctcttta gtgtcggttt caaacgcatg ttcacctttg gcatcgatga tggtgacggt
     1381 gttctggtcg ctgcccattg cggtgtttgc gaggttagcc acaatcatgg ggacgcgttt
     1441 tgccagtctt ttttgtctgg cgtgctcgag gacctgctcg gtttccgcag caaagccaac
     1501 gcagtaggga ggattgggta aggaggcgac gtcggccagg atatctttgt ttttaatgag
     1561 tttgagtgta agtgattctt cacttttttt gattttttga atggacgaag actctggacg
     1621 gaagtctgca accgcagcaa caccaatgaa tatgtgctgg tcggcgatgt gttgcatcac
     1681 ggtctgatac atctcattag cactgccagc ataatatgcg tgactgacca cagggcgagg
     1741 cacttgggca tgaccgtaga ccagggttac ttcggcaccc atacgcatgg caacctgtgc
     1801 tagcgcatag cccattttac cgctgctgag gttggtgatg cctcgcactg ggtcaagcat
     1861 ttccatggtg gcgcctgcgg taatcagaac ttttttactc gccagcagtt taggcgtaaa
     1921 aaatgcctgc aagtcatcca gtaatgcttc aggctccagc attcttcctt ggccaacttc
     1981 cccacatgct tgttcgccac tctctgggcc cagcacggtg atcccgtcct ggtttagttg
     2041 tgcaatattt cgctgggtcg cagcgttttc ccacatttgc ctgttcatgg ctggcgccac
     2101 cagaagtggg cactcacgtg caaggcatag tgttgagagt aaatcattgg caaagccatg
     2161 tgccaatttg gcaagaaagt ctgcgctggc aggcgcaacc agaatggcat ctgccttgcg
     2221 gcttagctcg atatggggca tgccattggc aatccgagag tcccaggcgt ccacaaagac
     2281 tggattgcct gacagtgcct gcaaagtggt cggggtgatg aagtgcgtag cggcttctgt
     2341 catcactact tgcacgctat gtccctgttt gattaggagg cgcaccagtt ctgcagtttt
     2401 gtaagctgca atgccgccgg taataccgag cagcagatgt agagaggagg cgtgcgtttt
     2461 agtcatgggc atattttaac tggaaagcgc ccgcagttac caattgggta aggtgtggca
     2521 ttttctttaa acatgttaac atttggtcgt tgacgggatg aggcgcatga tcaagttagt
     2581 caggattttt ttctggggag tggtggtgct gacggcacta atgcagctct ggcaggagta
     2641 tcagactttg caatggcgca aaccgttgaa tgtggcctta tacccgatca atgccgatga
     2701 tgcaccgcaa gtagacacat acattgctaa tttgcgcaca aaagatttcg acgtcatttc
     2761 acagtttttt gagcgcgaag cccttaaata tcatttgccg ttacagtatc cggtgcatct
     2821 ttacctgggg ccgaccattc aggacgtccc atctgcacct ccggcagtga acggtcattt
     2881 actcgatatt attctctgga gcctcaagtt tcgatggttt agttggagac atcaaccgcc
     2941 cgttggcgtt ccgattgata tcaagcttta tttgctatat cacgatccgg ccagacataa
     3001 gcatttgctg cattcgaccg cactacataa aggcaggata ggccgtgtca atctatttgc
     3061 tgaccatcgt cagcatgcgg tgaataccgt catcatcgca cacgagttgc tacacacgtt
     3121 gagtgcgagc gataagtatg acttgaatgc gaatctgccg ttgtttcctc aagggtatgc
     3181 agagccttat ctgcaacctg tttatccgca gcaattggcc gaagtgatgg ccggtaaagt
     3241 gccaatcacc gcgcaccgtg cgcgtatggc gaaaggcttg gaagaaacca ttatcggtag
     3301 accaactgcg cgtgaaatag gttgggtcaa ataaaaatca acgggggagt ccatggcaat
     3361 tacagattgg cctgagcagg agcggcccag ggaaaaatta ttatttgcag gggcaactgc
     3421 cttgtctgac gccgagttgt tggcgatttt tttgcgcgtt ggcgtgcccg gaaaatcggc
     3481 tgtcgatctg gcgcgggact tattgcacca ttttaaaagc ctaaatggca ttttttcagc
     3541 cagcttacgc gatgtgcaag tcgtacatgg catgggtgtc agtaaatatt gccaattgca
     3601 agccgtactt gaaatgagta agcgtgcgtt aggggagagc ttgcagcaaa cagacagttt
     3661 ttgctcgcca acgcaggtca agcattattt gcaactgcaa ttgtcctcac tgcagcggga
     3721 agtgtttggc attatgtttt tggatgcgca aaaccggttg attacttatg agaccctgtt
     3781 tcagggcagt ttaatgcaga ccagcgtcta tccgcgagag gtggtcaagc gcgctttggc
     3841 gttaaacgcg gcggccttga tattgagcca caatcaccct ggaggtagtg caaaaccaag
     3901 tctggctgat gagcaactga cgcagagttt gaaagatgca ttaagcctgg tcgacatcaa
     3961 agtgctggac cacattgtgg tatccgggca ggcaacgttt tcgtttagtg agatgggatt
     4021 aatctaggat tgcaggcagt gggatggtgg gtgctaacgg ggtcgaaccg ctgacattcg
     4081 ccttgtaagg gcgacgctct accaactgag ctaagcaccc atgccttgca acgaagggcg
     4141 cattataccg aaactctttt ttcctgcaag agcccgtcaa taaatattga tggaattgtg
     4201 agttgcgcta gttaaacggt tagccgtttg gtgatattga ttaactggag tgacatatcc
     4261 accatgccgg ttttacgttt gtctgccatt tccgacagta aagaatacgc ttgctcctcg
     4321 ctcaaatcgc gctgatgcat caggatgtcc ttggccgagt cgaccaactt ttggtcttcc
     4381 gggctcaata ggatgcgtgg tgttgagcta agtgacgcgg ttgtgtctgg agataagggt
     4441 ctcattggga atcatcaaat aaacttctat catcatttag caatctgcat gccaataggt
     4501 gaattttttt taacattatg attataaaga gtaatttatt tttttgtggc attggggaag
     4561 cgtctgtgca tgatgccgat tgcaccacgg tagtgccctt gcaccatgga accaaggctt
     4621 gatgagtgtg atgtttttgc gcagctccgg cctttttgtt acagtgcaaa tcattaaaaa
     4681 acaggaaaga attccatcac tatgaaagct ttttggagtc gtgcaggctg ggcaagtttg
     4741 gttttgttgg gtgcagcggc attttcaaat gttgcgctgg cgagggggga gtctctgaat
     4801 gcgctttggt taattactgc ggccgtgtgc atctatttaa tcgcgtatcg tttttatgca
     4861 gcatggattg ccgccaatgt gttggccatt aatgcgacgc gtgcgacacc ggctgagcgg
     4921 ctcaataatg gccgcgatta tgtgcctacc aaccgctggg tggtttttgg gcatcatttc
     4981 gccgcgattg ccggcccggg gccattagtt ggtcccactt tggcggcaca atttggttat
     5041 ttgcctggta ctttgtggat attgattggt gccgtacttg gtggcgcggt acaagatatg
     5101 gtgacgcttt tcttatccac gaggcgcaat ggccgtagcc tggggcagat ggcgcgtgat
     5161 gaaattggtg cgataggggg aacagctgcg ctgattggta cctttatcat catgattatt
     5221 ctgatcgctg tactggggtt ggtcgtagtc aatgcaatga agcatagtcc ttggggcacg
     5281 tcgactgttg ctgccaccat tccgattgcg atgctggtcg gcgtgtatat gcgatacctg
     5341 cggcctgggc gcgtgcttga ggcctcgctg ctgggggtgg ctttgttgct tgcgtctgtt
     5401 gtttttggtg gctgggtcga ccatcatccg caattggcca ccttctttga tcatgaaggg
     5461 ttgccactgg catttagtgt tattgcctat ggttttgctg cagccgtatt gcctgtttgg
     5521 ttgctgttgg caccgcgtga ttatttgtct acttttatga agttgggcac agtcatcatg
     5581 ctggcgattg ccatcctgtg gttgcatccc gagattcata tgcctgccat cacgcctttt
     5641 attgatgggt ctgggcctat ttttggtggc aagttgttcc cgtttgtgtt tattaccatt
     5701 gcttgcggtg cgatttctgg ttttcatgca ttgattgcaa gtggaaccac gccaaaattg
     5761 attaccagtg aaaatgatat ccgtgtcatt ggttatggcg caatgttgct ggaaagcttt
     5821 gtcgcagtga tggctatggt ggccgcgact gtactggaac cgggtgtcta ttttgcaatc
     5881 aatagccccg caggcgtggt tggtaaagag gcattggatg cagtgaacac cattagcagc
     5941 tggggttatc aggtgaccgt ggagcaaatg cagcatcttg ccagcgctat gggggaaagt
     6001 accttgtttg ccagaactgg tggtgcacca agcctggctg tggggatggc gagtattctg
     6061 gggaatgtat ttggggagca tatgctggca ttgtggtatc actttgccat catgtttgag
     6121 gcgattttta ttctcaccac tttagatgcc ggaacccgcg tcggccgttt catgctgcaa
     6181 gatatgctgg gtaatttgca tcccaagctg ggagaaacct catatacgcc gtctgtgatt
     6241 ttgacgagtg cactcgttgt cgctggttgg ggttactttc tttatatcgg ggtcattgat
     6301 ccgaatggtg gggtcaatat tttgtggcca ctatttggca ttgccaacca gatgctggct
     6361 gcaatcgcct tgtcggttgc tacaggcatc ctgatcaaat ccggtaaagc ctctaaagcc
     6421 tggatcactg gcttgcctct gctttggctg ctgaccatta ccacgacagc tgtctatgaa
     6481 aaagtgttca gtgatgatat ccgggtaggt ttttttgcag ccgcaaacga tttggcgcaa
     6541 aagttgtctt ttggagtgtt gccacctgaa aaagctgccg tggcaccgca attgattttc
     6601 aaccagcagc tggatgcttg gcttaccata ttttttgtgc tgatgttgtg gatcgttgtg
     6661 ctggatatgc tgcgcatgag ctggcgcagt ttggcgggaa aagtggtgct gtccagtagt
     6721 gaagcaccat atgtggcaag ccggttgggt taaggagagt caatatgcgc aagttgaaat
     6781 tgttttggca gcgagtccga cagttgtctg gtgacgatgc atatgagcgc tacctggccc
     6841 actacaatga gcatcatgca cagcctggcg cagccgcacc tttgacgcgg gctgattttt
     6901 ttaagcaatg gcaggatcaa aagtggacag gtatcaaacg ctgttgttaa gcgaagggga
     6961 tatttcgcgc atcattgaaa tggcgtggga agatcgcaca ccatttgagg cgattgagca
     7021 tcagtttggc ttgaggcagg cggatgtcat tgtactcatg cgcaagcagt taaagcctag
     7081 ggtatttgcc tcttggcgca agcgcacacg cgggcagcag accaagcatg cctccttgcg
     7141 gagcccgaat gtcaatcggc actgctcatc gtttcagcac aagcaagtgc ggccttagta
     7201 ttttttatcg tctgcgttga atgtaacaag tctgtttcag tcgcgcaggc ggctgcggat
     7261 tcagttaaaa taatatttta acgactatcc tagtatttct catgcctaaa taccgttctc
     7321 acacgacgac acaaggtcgt aacatggctg gtgcccgcgc attgtggcga gccaccggaa
     7381 tgaaagatga agatttttcc aaaccaatca ttgcggtagc caactccttc actcagtttg
     7441 tgcctggtca cgtccacttg aaagacttgg ggcaactggt cgcgcgcgaa attgagaagg
     7501 ctggcggcgt ggccaaggaa ttcaatacca ttgccgtgga tgatggcatc gccatggggc
     7561 atggtggcat gttgtatagc ttgccaagcc gcgacttgat cgcggatagc gtggaatata
     7621 tggtcaatgc acactgtgcg gatgcactgg tgtgtatttc caactgtgac aagattaccc
     7681 ccggcatgct gatggcggct ttgcgcctga acatcccagt ggtatttgtg tctggcggcc
     7741 cgatggaagc tggtaaagtg aactggcaag gccaggtgcg taaactggac ctcgtcgacg
     7801 ctatggtgga agcggcagat agtcatgtca gtgatgctga ggtggctgag gtggagcgtt
     7861 ccgcctgccc aacgtgtggt tcctgctccg ggatgtttac cgccaactcc atgaactgcc
     7921 tgactgaagc gctgggtctg agcttacctg gtaacggttc gacgctggca acgcacgccg
     7981 cccgcaaaca gcttttcttg caggcagggc gcctgattgt agacctcgcc aaacgccatt
     8041 atgaacaaga tgattacacc gtgttgccac gcaatattgc caccatgcgc gcattcgaga
     8101 atgccatgag cctagatgtg gccatgggcg gatcaaccaa caccgtattg cacttgctgg
     8161 ctgccgcgca tgaagccggg gttgatttca ccatgaagga tattgatcgc atttcgcgtc
     8221 aggtgccttg tgtgtctaaa gtggctcctg ccaccagcaa gtatcacatg gaagatgtgc
     8281 accgtgctgg cggtgtcatg ggtatcttgg cggaactcaa tcgcgccggt gtcatccata
     8341 ctgatgtgcc aaccgttcat agtacaagta tgggcgaggc gttagcaaag tgggatattg
     8401 tcacgacgca ggacgaagag gtgaagaaat tctaccgtgc tgcgccaggt ggtattgcta
     8461 ctacgattgc attcagtcag agcatgcttt ggccagagtt ggatgatgac cgtgagaacg
     8521 gctgtattcg taacaaagaa catgcctatt cacaagatgg tggtttggca gtgttgtacg
     8581 gcaatattgc ccaggatggc tgtattgtca aaaccgctgg cgtggatgac agtattctta
     8641 agttcactgg gcgtgcacgt gtatttgaaa gccaggattc agcggttgaa gcgattttgg
     8701 cagacaagat tgttgctggg gatgtggttg taattcgtta tgaaggcccg cgtggcggcc
     8761 caggaatgca ggagatgttg taccccactt cctatttgaa atccaaaggt ctgggcaaag
     8821 cctgtgcctt gttgaccgat ggccgtttct ctggaggcac gtcaggattg agtattggtc
     8881 acgcctcgcc agaagcggcc gaaggcggtg cgattggctt ggtccaggaa ggcgatacca
     8941 ttgagattga tatccctaac cgtacgattc atttggccgt gagtgatgag gagatggctc
     9001 accgccgcag tcatatggaa gcgaaaggcc acgatgcctg gaaaccatta aaccgcgagc
     9061 gcttggtctc acctgcacta cgtgcttatg cggccatgag taccagtgcg gctaaagggg
     9121 cggtacgtga tgtcgcccaa attgaaagga gaagataatg aataacgaat gcggcatcct
     9181 ctccaagagt atggaaatga ccaagcgcat gaagttgcat gcgcatgtga accagtggca
     9241 ggatgaaaat ggcttgcagt acctggagat tgataatccg cttgcgacag gcaaaattgc
     9301 cttgcaaggg gcgcatgtca tgcattggca gccaaaattc ctggcgcatc ccgtactttg
     9361 gttatcgagt aatgcacgtt atgtgaaagg gcgctccatc cgtggcggta cgcctatctg
     9421 ctggccatgg tttggagcgc acccgactga cagcacactg tgtccgcacg gctttgcgcg
     9481 tgtcatcccg tggcgcgtaa tggatattga cgcgacgcct accggtgcca cgcgcatcat
     9541 tttggaaatg cagcaaacgc cagaagcaca acgtcagctg tcttatccat atgaattgac
     9601 tctaaccatt accattggtc gccgtttacg aattgatttg gcgaccacca acttggcaga
     9661 gcatccattt gtgattggtg aagcgttcca tacctacttt aatgtcagcg acatctctaa
     9721 tattcaaatc actggcatgc aggatctggt gtatctggat aagctactca agtatgaacg
     9781 taacgtggag cataacgcgc tgagcttcga tgggcaggag tacgatcgcg tctacgttga
     9841 tcatagttcc gactgtgcca tacatgacag cggttataac cggattattc atgttgccaa
     9901 gtccggcagt gatacgacag tcgtctggaa cccaggcgca gaaaaggccg gcgccatggg
     9961 cgacatgggt gtggatgatg agtggcgtaa aacgatttgc gtagagacca cgaatgcgct
    10021 ggataacatg gtggtgatta atcctggccg caaacatacc ctcagcgcag agtattctat
    10081 tgagacgctg taacattgct gtgacacgtg tcaaccgtta agttgcacgt gcgtttttag
    10141 catgttggca aaacgccaaa ccggttgaaa ttatgtgaac tgccgtgatt tttagcggtt
    10201 tttctattgt agattttgct tggtcaggta atatgccaca tgcatgagaa ttgcattttg
    10261 gggttctatt catttagagg atattcaagg agatcgcatg aaagttttat tttccgcaat
    10321 cgcagcagca acattgttga tggctggtac agcgcacgct gtagatgccg ctgcagctaa
    10381 agcactggct caaaagagcg gttgtttggc atgtcacagt gtggatgcaa aagtattggg
    10441 cccagcatac aaagatgttg cagctaaata caaaggcgat aaaggcgctg aagctaaatt
    10501 gatcgctaaa gttaaagctg gtggtagcgg tgtttggggc aatatcccaa tgccagcaaa
    10561 cagcccacaa gtgaaagacg aagacatcaa gaccatcgtt gagtgggtat tgtctctgta
    10621 atttcagcct tgctgtaata gaaaaagccg actgtatagt cggctttttt attgggcatg
    10681 tttttaaatg tgtcacgacc gtaatttact gtgtattaat gagtaaatga aatagaacac
    10741 gacaccgacg acgccccagt tgagaaagcg atgccaggtt tc
//
LOCUS       DATMSK010000068         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_146895,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000068 DATMSK010000000
VERSION     DATMSK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146895"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VNZ84_02250"
     CDS             complement(<1..244)
                     /locus_tag="VNZ84_02250"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985370.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWU65800.1"
                     /translation="MSDNSTHEQPARERTAKKVAGVKEIDAAKTSRIPIKIVPQPMLR
                     KPEWIRMKVPDSARFQEIKQVLRDNNLHTVCEEASCP"
     gene            complement(234..848)
                     /gene="lipB"
                     /locus_tag="VNZ84_02255"
     CDS             complement(234..848)
                     /gene="lipB"
                     /locus_tag="VNZ84_02255"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWU65801.1"
                     /translation="MSALEIRQLGLTDYAATLVDMQTFTAQRGPETPDQLWITEHPPV
                     YTLGLNRRGVRLPQNDIPVVNVDRGGKITYHGPGQLIIYCLIDLARIHLNVRQLVSIL
                     ENSLIEFLVAHSITAEARADAPGVYVEGKKIASLGLRLKNQCCYHGLSLNIEMDLQPF
                     ADIDPCGYAGMQMTQTRDLNIALQMPDIAADLTQRLKEKLAHVR"
     gene            complement(863..1150)
                     /locus_tag="VNZ84_02260"
     CDS             complement(863..1150)
                     /locus_tag="VNZ84_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HWU65802.1"
                     /translation="MTKFETMPPVEETLIEFPTNFPIKVMGETHDDFAMEITRTIQAH
                     WPEFDTHLIEIRGSSNGKYLSLTCLVYVTSKPQLDDIYRSLTSHPMVKVVL"
     gene            complement(1178..2275)
                     /locus_tag="VNZ84_02265"
     CDS             complement(1178..2275)
                     /locus_tag="VNZ84_02265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWU65803.1"
                     /translation="MTVAAEAITGNVPTPTLAAKAFVLRDANTGSLLAASNPDMPVEP
                     ASLTKVMTAYLTFEAVKQKRLSLLQTLPVSQKAWKVEGSKMFIDTTMTVTVDELLHGL
                     IIQSGNDAAITLAEGIAGSEELFATLMNKKAIALGMKNSHFINATGLPDPTHTTTARD
                     LSILANALIRDFPQDYARLYSQKSYTYNKITQPNRNRLLFLDPTVDGMKTGHTESAGY
                     CLISSAKRNDFRLISVVLGTTSDNVRAAESQKLLNFGFQFYESKLIYPANKAITRQKV
                     WKGTESEVDLTVAKPVYLTLPQGEYANMKANLRITKPLEAPLKAHQAVGAVDFSLGGK
                     VVHSVPLVTVHAVEASNIFSRMIDQVRLYLQ"
     gene            complement(2343..>3036)
                     /locus_tag="VNZ84_02270"
     CDS             complement(2343..>3036)
                     /locus_tag="VNZ84_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWU65804.1"
                     /translation="VNGETVTPMSEIRTPFTQTGHASWYGKKFHGRKTASGEPYDMFK
                     LTAAHRTLPIPSYVRITNLSNGKTVMARVNDRGPFGRDRIIDLSYAAAKQLDIIRHGS
                     AQVEVTLIDPSQPIDSTVTSAPPNADIQPTAKEVVQVTRTITSAKPASALDANGVYVQ
                     VGAFGQEENADRLQQRIFSALPETQSLLNKVYNGKVFQVVLGPYPDHALAAQAASQMR
                     DQLQLPALIFSR"
BASE COUNT          667 a          728 c          842 g          799 t
ORIGIN      
        1 tggggcaact ggcctcttca cacaccgtat gcaggttgtt gtcgcgcagt acctgtttga
       61 tttcctggaa gcgtgccgaa tccggcactt tcatgcgtat ccattccggt ttgcgtagca
      121 tgggttgcgg cacaattttg atgggaatac gtgaagtctt ggccgcatca atttctttga
      181 cgcctgcgac cttcttcgct gtgcgctctc tcgcaggctg ctcgtgggtc gaattatctg
      241 acatgcgcca gtttctcttt caatcgttgg gttaaatcgg cagcaatgtc gggcatttgc
      301 agcgcaatat ttaaatcccg ggtttgtgtc atttgcatgc cagcataacc gcaagggtcg
      361 atatcggcaa atggttgtaa atccatctca atattcaagc tcaagccatg ataacagcac
      421 tggtttttca ggcgtaatcc cagcgaagcg atttttttgc cttccacata caccccaggc
      481 gcgtctgcgc gggcttcggc tgtgatgcta tgcgccacca gaaactctat caggctgttt
      541 tccaagatgg agacaagttg ccgtacattc aagtggatgc gtgcgagatc aatcaggcaa
      601 taaataatga gttggcctgg accgtgatag gtgatcttgc cgccgcggtc cacattgacg
      661 acgggaatgt cgttttgtgg caaacgtacg ccacgacggt taaggcccaa ggtatacacg
      721 ggaggatgct cggtgatcca gagctggtca ggcgtctctg ggccgcgctg cgcagtgaag
      781 gtttgcatgt cgactagcgt cgccgcatag tcagtcagac ccagctggcg gatttccagg
      841 gcagacatga aagaaaagag tgttacagca caaccttgac catggggtgc gaggtcaggc
      901 tgcgataaat atcgtcgagt tgcggtttgg aggtgacgta taccaggcag gtcaggctca
      961 ggtatttacc attgctactg ccacgtattt caatcaggtg tgtgtcaaac tctggccagt
     1021 gtgcctgaat cgtacgcgtg atttccatgg caaagtcgtc atgtgtttca cccatgacct
     1081 tgatcgggaa gttggtagga aactcgatca gggtttcttc taccggaggc atagtctcga
     1141 acttggtcat ggttacctac ctttgtgaat gatggtctta ctgtaaatac aggcgcacct
     1201 ggtcgatcat gcgtgagaag atgttgctgg cttctaccgc gtgtacagtg accagaggga
     1261 cgctatgcac aactttgcca cctaggctga aatcgactgc gcccactgct tgatgcgctt
     1321 ttaacggcgc ctctagcggt ttggtaatgc gcaggtttgc cttcatattg gcatactcac
     1381 cttgcggcaa agtcaaatag acaggtttgg ccaccgtcaa atccacttcg ctttcagtcc
     1441 ctttccacac tttctggcgc gtaattgctt tgttggctgg gtaaatcagc ttggactcat
     1501 aaaactggaa accaaaattc agcagttttt ggctttcggc tgcccgtaca ttatcggatg
     1561 tggtgccaag gaccacagaa atcaagcgga agtcgttccg ttttgcagat gaaatcaggc
     1621 aatacccagc agattcggta tgcccggttt tcatgccatc gacggtgggg tcaagaaaca
     1681 gcaggcggtt acggttaggc tgcgtaattt tgttataggt gtaagacttt tgtgagtaca
     1741 ggcgcgcgta gtcttgtggg aagtcgcgga tcaaggcgtt ggccaggata gacaagtcac
     1801 gggcagtggt ggtgtgtgtt gggtcaggta agccagtggc attgataaag tggctgtttt
     1861 tcatccccag tgcaatggct tttttgttca tcagggtggc aaacagctct tcactcccgg
     1921 caattccttc tgccagggta atggccgcat cgttgccgga ctggataatc aggccatgca
     1981 gcaactcatc cactgtgacc gtcatggtgg tgtcgataaa catttttgag ccttcgacct
     2041 tccacgcttt ttggctgacg ggcaaggttt gaagcaaaga taatcgtttt tgcttgaccg
     2101 cttcaaaggt caggtaagcc gtcatgacct tggtcaatga cgccggctct acaggcatat
     2161 ccgggttgct ggcggccagc aggctgccag tgttggcatc gcgtaacaca aatgccttgg
     2221 cggccaaggt cggggttggc acattgcccg tgatggcttc agccgccaca gtcattaatg
     2281 gaatggaaag tagaaaagca atacaagaac gccacatggt catcatttaa tgagaaaaat
     2341 acttaacgag aaaatatcag tgcaggtaac tgcaattggt cacgcatttg actggctgcc
     2401 tgtgctgcca atgcgtgatc tggataaggc cccagcacga cctggaacac tttgccatta
     2461 tacactttgt tgagcaggga ttgggtttct ggcaaggcgc tgaagatgcg ttgctgcaag
     2521 cggtctgcat tctcttcctg gccaaacgca ccgacttgta cgtatacccc attcgcatcc
     2581 agtgctgaag cgggttttgc tgatgtaatg gtgcgagtga cttgcacaac ttctttggct
     2641 gtgggctgga tgtctgcatt cggtggggca gacgtcacgg tgctgtctat tggctgtgaa
     2701 gggtctatca atgtcacttc aacctgggcg gagccgtggc ggataatatc cagctgtttg
     2761 gccgccgcat aggacaggtc aataatgcgg tcgcggccaa agggaccacg gtcgttgacg
     2821 cgcgccatca ctgttttgcc gttacttaga ttagtgatgc gtacatagct gggaattggt
     2881 aaggtcctgt gggccgccgt caatttgaac atatcatacg gctcgccgct ggctgttttt
     2941 cgcccatgaa acttcttgcc ataccatgag gcatgaccgg tctgcgtgaa gggcgtacgg
     3001 atttccgaca tcggggtcac ggtttcccca ttcacg
//
LOCUS       DATMSK010000069         6321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_334959,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000069 DATMSK010000000
VERSION     DATMSK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334959"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..948
                     /locus_tag="VNZ84_02275"
     CDS             187..948
                     /locus_tag="VNZ84_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWU65805.1"
                     /translation="MQLHYQVHAQNQGKGPPLVLLHGLFGSADNWGSIAKHFSQHYQV
                     ISVDLRNHGRSPHSESQTYPEMADDLSALCDALGLAQIHLLGHSLGGKVAMQFATQYP
                     QKVSKLIIVDMAMRAYQDRYTQLMDHMLAVNLSQMASRNEVDKALADAIPNVRVRQFL
                     LTNLVNHEGQLQWRINLPALKANYATLIAAVNVHFDQPSLFIRGERSDYVNARDITEL
                     QQHFPQAQFVTLPTDHWVHAEQPQLFIQALEAFLA"
     gene            complement(1005..1538)
                     /locus_tag="VNZ84_02280"
     CDS             complement(1005..1538)
                     /locus_tag="VNZ84_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWU65806.1"
                     /translation="MARIQTINRETASAEVNTTLDAVKAKMGKVPNLIATLAQAPVAL
                     NAYLSLSEAASKGRLSAKQRESLALAIGQANSCQYCLSAHSLIAKGAGLNNEAIQSAR
                     QARAEDALTDALLKLAVKIVQQRGLLSDEDFAEAYLAGVDDGLVLEVITSVALNTLTN
                     YTNHIAQTDIDFPVVTL"
     gene            1642..2451
                     /locus_tag="VNZ84_02285"
     CDS             1642..2451
                     /locus_tag="VNZ84_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWU65807.1"
                     /translation="MDPLLPLLNQISLSAQVFFTGDLCQVVNFDGTGAVGHIHLLRAG
                     KLDLMFRDKRMAHIDQPSVVFFPKPTIHVLNPVEGEPCELVCAMINLGANVRSPLATA
                     LPEYVIVPLNDMLQIAPTLQLLFHEAFAQDLGRQAALDRLAEYFLIQILRHVIQHGQL
                     QQSIFAALADTRLALAVKAMHENPSHPWTLETLADASGMSRARFAANFKTTVGTTPLD
                     YLTDWRLSIAQNLIRQGKPLKSIATQVGYQSTAAFSRVFAKRLGVSPTEWR"
     gene            2572..3099
                     /locus_tag="VNZ84_02290"
     CDS             2572..3099
                     /locus_tag="VNZ84_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWU65808.1"
                     /translation="MAQQDSVITETFNKEKARLRNFIRKSVPNAGDADDILQDVFYEF
                     VSAYRLPEPLEQVGAWLYRVARNRIIDRFRKQKQEVSLEQNDDDDALWLESILSIETE
                     TPASIYERKMLVEYILAALNQLPSEQRDVFIAHELDGKSFKQIAAETGVAINTLLARK
                     RYAVTFLREQLQTSD"
     gene            3132..3443
                     /locus_tag="VNZ84_02295"
     CDS             3132..3443
                     /locus_tag="VNZ84_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65809.1"
                     /translation="MRNCKSKCAAFAALAVVCIAALSYVVMLLWNHVVVSLADGIHVI
                     DYCHAVGLLILSRILFGGFRGRHGHHHRHCGGEAMSADEQEKVRCGLFSRFKSKRDVE
                     S"
     gene            complement(3515..4420)
                     /locus_tag="VNZ84_02300"
     CDS             complement(3515..4420)
                     /locus_tag="VNZ84_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU65810.1"
                     /translation="MRDINDYILFAEVVSGGSFAAASQRVRIPKSTISRRIGLLEERL
                     GVRLIERSTRRFRVTEIGQSFYERCKVIMLDVEQADSIVSESLSEPKGLVRCSCPLGL
                     YETLSPIFSSFLLAFPQARLQLVAGDNPVNLIEDRIDIAIRVRTVLDTDGSLVMRTLG
                     HSERILVASPTLASKMDQSSLSNLSAAPTLATSDQNHSLEWQFHHASLGTQSVWHDPV
                     LSCVDFATVREAAIAGIGVALLPDHTCKQDLENGKLVRVFPEWKTTLGILHIVFTTRR
                     GLPPVVRAFIDHLASQIKQTGTITS"
     gene            4538..5293
                     /locus_tag="VNZ84_02305"
     CDS             4538..5293
                     /locus_tag="VNZ84_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU65811.1"
                     /translation="MSATFENKVVVVTGGTSGIGLATAKHFANQGANVYITGRRQAEL
                     EAAIKTIGGKVKGVQADISKLADIDKLYDAVQQNHSQIDVIYANAGGGNMLPLGAITE
                     DHYEDIFGRNVKGVIFTVQKALPLLKDGASIVLTGSTTSVKGTAAFSIYSASKAAIRN
                     LARSWILDLKDRGIRVNVVSPGPIKTPGLVELAGNDPAQQQGLLDYLGAQVPMGRVGN
                     PAEVAKAVAFLSSPDASFINGIEFFVDGGQAQI"
     gene            complement(5353..>6321)
                     /locus_tag="VNZ84_02310"
     CDS             complement(5353..>6321)
                     /locus_tag="VNZ84_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWU65812.1"
                     /translation="VLAIGLLLQCLAYANTSVAGAELPTASVVKYHAESGYYTFKLGT
                     YEITVISDGTVPQDLHVLLKGAPQQTVDGLLNQVHLHNPVEASINVFLINTGKKLILV
                     DTGAGDFFGKGFGGKFSERLNKLGVKPEDISDILITHIHTDHTGGLVHEGKAVFPNAN
                     IYVGKADIDFFLDTKNQNGVNGYGKAYFQQAQTAMQPYVALGHVKPLSGAQKILADFN
                     AYPTPGHTPGHYYYRLESQGQSITFIGDSVHVEAVQFPHPDITITYDVDQPCAAKQRL
                     AQFTQLAKSNAMIAAPHLPYPGIGYINTQKEGFAFIRAEFHDRDGL"
BASE COUNT         1640 a         1533 c         1516 g         1632 t
ORIGIN      
        1 tgattcctcg ttaatgtgtg aatgaggctc cattatgaaa aaaacatttt cacttgataa
       61 tgaggcggtt tctgaacttg atgtcagact tggctgacgc gtttgaagga ttttaaacat
      121 gcctgtaggg cacctgttta acgctaaaat agcggcttat attttaatac agtcagcagt
      181 ttcaccatgc aattacatta tcaagttcac gcccaaaacc agggcaaagg cccgccactc
      241 gttctgctac atggcttgtt cgggtctgct gataactggg gcagcatcgc caaacatttc
      301 tcgcagcact atcaggtgat cagcgttgat ttgcgaaatc atggccgctc gccgcatagc
      361 gagtcgcaaa cgtaccctga aatggcagat gacttgtcag cgttatgcga cgccttaggg
      421 ttagcgcaaa ttcatttact tggccattct cttggcggca aagtcgccat gcaatttgcc
      481 acgcaatatc ctcaaaaagt cagcaaactc attatcgtcg atatggcgat gcgcgcttat
      541 caagacaggt acacgcagct catggatcac atgctggccg tcaatctcag ccagatggcc
      601 tcgcgtaatg aggtagataa ggccttggcg gatgcgatcc caaatgtgag ggtaaggcag
      661 tttttattaa ctaacctggt taaccatgaa gggcagttgc aatggcgcat taatttgcca
      721 gcgcttaaag cgaattacgc cacattgatt gcggcggtga acgtgcactt tgaccaacct
      781 agcctgttta ttcgcgggga gcgcagtgac tatgtgaatg cgcgtgacat caccgaactg
      841 cagcagcatt ttccccaggc gcagtttgtc accttgccca cggaccattg ggtgcatgcg
      901 gagcagccgc aattgtttat acaggcgcta gaagcttttc tggcctgatt taggcttggt
      961 tcaaaaacaa tcaggccacg cgctggtggc ctgaattgcc tggcttataa agtcacgacc
     1021 gggaaatcaa tgtcggtctg tgcgatatgg ttggtgtagt tggtcaaggt gtttaacgcc
     1081 acgctggtaa tcacttctaa caccaggcca tcatctaccc ctgccaaata agcctctgca
     1141 aaatcttcat ctgaaagtag cccgcgctgc tgcacaattt tgacagccaa tttgagcaat
     1201 gcatcagtca acgcatcctc tgccctggct tggcgtgccg actgaatggc ctcgttattc
     1261 agacctgcgc cttttgcaat caggctatgg gcggacaagc aatactggca actattcgcc
     1321 tggccaatcg ctaaagccag tgactctctt tgttttgcgc ttaagcggcc tttgctcgcc
     1381 gcctcactca aactcaggta agcatttaag gcaacagggg cctgtgccaa tgtggcaatc
     1441 aaattaggca ctttgcccat tttggctttc acagcgtcca gtgtggtgtt cacttctgcg
     1501 ctagcggttt cgcggttaat cgtttgaata cgtgccatga tgaatctcct tgtgtggtta
     1561 attagaacgc ttggtgcgtt ggagatatca tgcaatactc atgattatta aaggtctcgt
     1621 tatgttgatc aggagtctcg tttggacccg ttattgccac tattgaatca aatctcgctc
     1681 tctgcccagg tatttttcac cggagatctg tgccaagtag tcaactttga tggcactgga
     1741 gcggtggggc acattcattt gttgcgggcg ggaaagctag acctgatgtt tcgcgataaa
     1801 cgcatggcac atatcgatca accgagcgtg gtgttcttcc ctaaaccgac catccacgtg
     1861 ctcaaccccg tagagggtga accgtgtgaa ttggtgtgtg ccatgattaa tttgggcgca
     1921 aacgtacgca gcccgttggc caccgcattg cctgaatatg tgattgtgcc tttaaatgac
     1981 atgctacaaa ttgcgccgac cttgcaactg ctgtttcatg aagcctttgc gcaagactta
     2041 ggccgtcaag cggcgttaga ccgattggca gaatacttcc tgatccagat cttgcgccat
     2101 gtgattcagc acgggcagtt gcagcaaagc atttttgccg cattggccga tacccggttg
     2161 gcgttggctg tcaaagccat gcatgagaac ccgtcccatc catggacact ggaaaccctg
     2221 gcggatgcgt caggcatgtc gcgcgccagg tttgccgcca actttaaaac cacggtaggc
     2281 accacaccgc tggattacct gaccgactgg cggctctcaa tcgcacaaaa cctgatccgg
     2341 cagggcaagc cgctcaaaag catcgccacc caagtgggtt atcaaagcac cgctgcgttt
     2401 agccgcgtgt ttgcaaagcg gctaggcgtg tcacccaccg agtggaggta aacatgctta
     2461 gcaagctgta tttaaaaata tttcagcctt catacaatat ttccatgtgt gaaacgtctt
     2521 cacagcatgg atatacattt agcaaataat caaagcttga cgcagataaa tatggcccag
     2581 caagactctg tcatcaccga aactttcaat aaagagaaag ccaggctacg gaatttcatc
     2641 cgcaaaagcg taccgaatgc cggtgatgca gatgatattt tgcaggatgt gttctatgag
     2701 tttgtttcag cctatcgttt gccagagcca ctagagcaag tcggtgcatg gttataccgc
     2761 gtggcaagaa accgcattat tgaccgcttt agaaagcaaa agcaggaagt ctcgctagag
     2821 caaaacgatg atgacgatgc gctatggctg gaatcaatac tttccattga aaccgaaact
     2881 ccggcatcca tttacgaaag aaaaatgctg gttgagtaca tattagccgc gctcaatcag
     2941 ctaccaagcg aacagcgtga tgtatttatc gcgcatgagt tggatggcaa aagctttaaa
     3001 caaattgccg cagaaaccgg tgtggctatc aacaccttat tagcaagaaa aaggtatgca
     3061 gtgacttttc tacgagagca actgcaaacc agtgactaat catattaatg tctgaattat
     3121 tggagaaata catgagaaat tgtaaaagta aatgtgctgc atttgcagcg ttagccgtcg
     3181 tctgcatcgc cgcattatct tatgtggtca tgctgttgtg gaaccacgtc gtggtttcat
     3241 tagccgatgg catccatgtg attgattact gccacgcagt cgggttgttg attctgagca
     3301 ggattctttt tggtgggttc agaggtcgtc atgggcatca tcatagacac tgcggtggtg
     3361 aagccatgag cgcagatgag caagagaaag tgcgttgtgg tttattcagc cgctttaaat
     3421 ccaaacgaga tgttgagtca taaccgcagc ctaagagtgt aagcctgatg aaaacggctg
     3481 ctagtcatag ccgccgtttt tttatttaat caaactaact cgttatagta cccgtttgtt
     3541 tgatctggct tgcaaggtgg tcaataaatg cacgcaccac tggcggcagc ccgcggcggg
     3601 tcgtgaatac gatgtgcaaa atgcccaggg tggttttcca ttccggaaaa accctgacca
     3661 atttaccgtt ttccaaatcc tgtttgcatg tgtggtcagg caataatgct acgccaatcc
     3721 ctgcaatggc tgcttctctc acggttgcaa aatccacaca gctcaatacg gggtcatgcc
     3781 agaccgattg ggtgcccaat gacgcatgat ggaattgcca ttctagggag tgattctgat
     3841 ctgacgttgc aagcgtcggt gctgctgaga gattacttag gctggattga tccatcttac
     3901 tcgctaacgt tggcgaagcg accagaatgc gttcggagtg acctaatgtc ctcatcacca
     3961 atgagccatc ggtatccaac acagtccgca cccggatagc gatatcaatt ctgtcctcga
     4021 tcaggttcac aggattatcc ccagccacca gttgcaatct ggcttgagga aaggcaagta
     4081 gaaatgacga aaaaataggg gagagtgttt catacaatcc cagtgggcag ctgcatcgca
     4141 ccagcccctt gggttcactg agcgattccg agacgataga gtcggcttgc tccacatcca
     4201 gcataatcac tttgcaacgc tcataaaacg actgcccgat ttctgtcacg cgaaatcgcc
     4261 tggtcgatcg ctcaattaaa cgcacgccga gtctttcttc cagtaggcca attcttctac
     4321 tgatagtgga tttaggaata cgcacgcgct gactggcagc ggcaaaactc ccgccactca
     4381 ccacctcagc aaacagaatg taatcattga tatcacgcat attggctcat taatggaaca
     4441 attaaactaa taaaagcagt ctagtgcatt tattggctca ctggcaatat ttcatccatg
     4501 cagtaaacgt tcatttcatt acttaaggag aaatatcatg tcagcaacat ttgaaaataa
     4561 agtcgtcgtc gttactggtg gtactagcgg tattggtctt gccacagcta aacattttgc
     4621 aaaccagggt gcgaatgtct atatcacagg ccgtagacaa gccgaattag aggccgccat
     4681 caaaaccata ggcggtaagg taaaaggcgt tcaggccgat atttccaaac tggccgatat
     4741 tgataaactt tatgatgctg tccagcagaa tcactctcaa atcgacgtga tctacgccaa
     4801 cgcaggtggc gggaatatgt tgccattagg cgccattact gaggatcact atgaggatat
     4861 ttttggccgc aatgtcaaag gtgtgatctt cacagtgcaa aaagcattgc ccttacttaa
     4921 agacggcgcg tccattgtat taactggttc aaccaccagc gtcaaaggca ccgccgcatt
     4981 tagtatttac agcgcatcca aagcggccat tcgcaacctg gcacgtagct ggattttaga
     5041 cctcaaagac cgcggtattc gtgtcaatgt cgtcagccca ggcccaatca aaaccccagg
     5101 cttggtagaa ctggcgggaa atgatccggc ccagcaacaa ggcctgctgg attatttagg
     5161 cgctcaagtg cctatgggga gggtaggcaa ccctgctgaa gtcgcgaaag ccgtggcatt
     5221 cctttcatcg ccagatgcca gcttcattaa cgggattgag ttttttgtgg atggtggcca
     5281 ggcgcagatt taacatagcc gagaaataaa ggccctcgtt gagagggctt tttctcatct
     5341 aaatccgcgt aatcacagtc catctctgtc atgaaattct gcacggataa atgcaaaccc
     5401 ttctttttgc gtattaatgt agccaatccc aggatacggc aagtgtgggg ctgcaatcat
     5461 ggcgttactt tttgctaatt gcgtgaattg agccaagcgt tgttttgcgg cgcaaggttg
     5521 gtcaacatcg taagtaatgg tgatgtcagg gtgaggaaac tgcaccgctt ccacatgcac
     5581 ggaatcgccg atgaatgtaa tcgactggcc ctggctttcc agccggtagt agtaatgtcc
     5641 tggcgtatgg cccggcgtgg ggtaagcatt aaaatcagca agaatttttt gtgcgccact
     5701 taatggtttt acgtggccca acgccacata tggctgcatc gcagtttgcg cctgctggaa
     5761 ataggctttg ccatagccat tcaccccgtt ttgattttta gtatccagaa aaaagtctat
     5821 atcggcttta cccacataaa tattcgcatt ggggaaaacc gctttgccct catgcaccag
     5881 cccgcccgtg tgatcggtat ggatatgggt aatcaatatg tcagaaatat cttcaggctt
     5941 cacgcctaac ttgtttaaac gctcactaaa cttgccgcca aaccccttgc caaaaaaatc
     6001 gcccgcgccc gtatccacca ggattaattt cttgccggtg ttgattaaaa atacatttat
     6061 cgatgcctca accgggttat gcaaatgcac ctggttcagc aagccatcca ccgtttgctg
     6121 gggcgcgcct tttagcaata catgtaagtc ttgtggcacc gtaccatctg aaatcactgt
     6181 aatttcatac gtacctaatt tgaatgtgta gtaaccagat tccgcatgat atttaaccac
     6241 tgaagccgtt ggtaattcag ccccggcaac agaagtgttg gcataagcca ggcactgtaa
     6301 caataagcca atggctaaaa c
//
LOCUS       DATMSK010000070        17780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_241138,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000070 DATMSK010000000
VERSION     DATMSK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 17780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17780
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241138"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VNZ84_02315"
     CDS             <1..1200
                     /locus_tag="VNZ84_02315"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWU65813.1"
                     /translation="RFGIPGMPGGPGQDGGQDYKTQSLGSGFIISADGYILTNAHVVN
                     EADEVLVKLYDKREFKAKIIGADKRTDVALIKIEASGLPKVTIGDPNALKVGEWVAAI
                     GSPFGLENTMTAGIVSAKGRALPQENYVPFIQTDVAINPGNSGGPLFNLRGEVVGVNS
                     QIYSKSGGSMGLSFSIPIDVAIDISNQLKTNGHIARGWLGIGIQEVTKDLAESFGLKQ
                     TSGALIAGVEKNSPADKGGLEAGDIILKFDGKPIVTSSDLPRVVAATKPGKVVPVEIV
                     RKGANKTLQLGVGEMPSEQGEVPAANKEPAKPEANRIGLTLRELTPQQKKKLNGKNGL
                     LVIESSGAAAQAGIRRGDVILGLNNNESQSVDLFNKQINAVAAGKTVAVLVQRGENTL
                     YVPIKIK"
     gene            1386..3179
                     /gene="lepA"
                     /locus_tag="VNZ84_02320"
     CDS             1386..3179
                     /gene="lepA"
                     /locus_tag="VNZ84_02320"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWU65814.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRIIQLCGGLEAREMEAQVLDSM
                     DIERERGITIKAQTAALQYKANNGETYFLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQSVANCYTAIDLGVEVTPVLNKIDLPSADPARVSQEIEDVIGVDATDAVR
                     CSAKTGEGVRDVLEEVVAKVPPPTGDAAAPLKALVIDAWFDNYVGVVMLVRVVDGVLR
                     AKEKIRLMATGAEYLCEQVGVFTPKSLQRPQLSAGEVGFIIAGIKELAAAKVGDTVTL
                     AGKPAAEPLPGFKEVKPQVFAGLYPVESNQFEALRTALEKLRLNDSSLQFEPENSSAL
                     GFGFRCGFLGLLHMEIVQERLEREYDMDLITTAPTVIYELLLKSGDVVQIENPSRLPE
                     PSRIEEIREPMIVVNLLMPQDYVGPVMTLCNNKRGVQRNMQYMGRQVMLSYEMPLNEV
                     VLDFFDKLKSVSRGYASMDYEFLEFRAADLVKLDIMVNGERVDALSLIVHRANSVYRG
                     RELVAKMRELIPRQMFDIAIQASIGANIIARETVKAMRKNVLAKCYGGDITRKRKLLE
                     KQKEGKKRMKQVGNVEIPQEAFLAILRVEDK"
     gene            3215..4030
                     /gene="lepB"
                     /locus_tag="VNZ84_02325"
     CDS             3215..4030
                     /gene="lepB"
                     /locus_tag="VNZ84_02325"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWU65815.1"
                     /translation="MYFAIFMLVILLVSGAIWLLDILVLRKQRQAGALGDVPDPWYVE
                     YSKSFFPVILLVFMVRSFVVEPFKIPSGSMMPTLLAGDYILVNKFTYGLRVPILNNVF
                     YPVSLPKRGDVFVFHYPPDTSIDYIKRVVGLPGDKIQYRDKQLTINGKVINLDFVQPY
                     QYALNVNDESGNARAVDVTASRLSEQLGDVKHDVLIHDIMNQYGPGSLGDRLMQGETI
                     TVPEGEYFAMGDNRDNSSDSRVWGFVAEKHLVGKAFFIWFNFDQWRRIGQSIG"
     gene            4078..4431
                     /locus_tag="VNZ84_02330"
     CDS             4078..4431
                     /locus_tag="VNZ84_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HWU65816.1"
                     /translation="MKFSKTSTQHGIGLIGFLLVLTAFGILGMVALKALPSYMEFFSI
                     QSTVNRIAHDPMLQNDSDIRKSFDRQMEVDSIHEVTGRDLEISGGVVAMRYQKKVPLT
                     DAMTLLIDFDATSKP"
     gene            4456..5127
                     /gene="rnc"
                     /locus_tag="VNZ84_02335"
     CDS             4456..5127
                     /gene="rnc"
                     /locus_tag="VNZ84_02335"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWU65817.1"
                     /translation="MNALPVLQTRISYQFAQLELLQQALTHRSFSASNNERLEFLGDS
                     VLNFIIAHLLFNLFPNLPEGDLSRLRAKLVKESSLAEIATDLHLGDALKLGEGELKSA
                     GWRRPSILADALEAIVGAVYLDGGFDAAQQVVALLYRDKLSTIDPKAIDKDAKSQLQE
                     YLQSKKMELPEYQVVSIEGEAHAQTFTVQCFIKKLKLTTTGVGTSRRVAEQQAAKLAM
                     DNIQS"
     gene            5124..6026
                     /gene="era"
                     /locus_tag="VNZ84_02340"
     CDS             5124..6026
                     /gene="era"
                     /locus_tag="VNZ84_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWU65818.1"
                     /translation="MTELTPAADDFRCGTVAIVGRPNVGKSTLLNHILGMKLAITSRK
                     AQTTRHRLLGIHTTEDTQFLFVDTPGFQQKHLNALNKSLNKTVTQVLTEVDVVLFVIE
                     PMKLGEADELVLKLLPKNKPVLLVVNKADLMGDKGNLLPLIQDFDLLFDFAGIIPVSA
                     KKNLYLDELLAAVREHLPQQPAIYAEDELTDKNERFLAAELVREKVFRLLGDEVPYSV
                     TVEIEKFETVKNLRRIFAAIIVDKDSQKPMIIGKGGEKLKQISTEARQDMEKLFGSKV
                     YLETWVKVKGGWADDARALKSLGL"
     gene            6053..6805
                     /gene="recO"
                     /locus_tag="VNZ84_02345"
     CDS             6053..6805
                     /gene="recO"
                     /locus_tag="VNZ84_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWU65819.1"
                     /translation="MAVHSPDSGSHKQSLQPVFILHTYPFKETSLVVEMFSRDLGRIA
                     AVAKGARRPLSAMRGILQSFQQLAGTWSGKNELKTLHGLEWMTGLTLLRGDALMCGFY
                     MNELLLRLLPRDDAHTQLFEYYAQTIQLLSTLQQDAGSGQLAEMMRRFELKMLQELGY
                     AVPLTHDEHGEVIHADEMYRFEADYGACAPSATKNGLLVQGATLLEMARGNYSSATTQ
                     SQSKQLMRYLLQHYLGEKPLHTRQLLVDLQNF"
     gene            6898..7626
                     /gene="pdxJ"
                     /locus_tag="VNZ84_02350"
     CDS             6898..7626
                     /gene="pdxJ"
                     /locus_tag="VNZ84_02350"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HWU65820.1"
                     /translation="MLKLGVNIDHVATIRQARGTRYPSVVQAALRAEQSGADSITLHL
                     REDRRHMQDADIFALRPLLQTKMNLECAVTSEMIDIAIQVNPQDVCLVPERREERTTE
                     GGLDVLTHYAKVEHAVKKLSDQGIRVSLFIAPDLAQIDAAAKMGAPVIEIHTGTFADA
                     ETEKQQQEELEKIQVALQHAKAADLVVNAGHGLHYHNVHHIARLPGFEELNIGHAIVA
                     HALFVGWDSAVREMKALMKEFSPR"
     gene            7655..8044
                     /gene="acpS"
                     /locus_tag="VNZ84_02355"
     CDS             7655..8044
                     /gene="acpS"
                     /locus_tag="VNZ84_02355"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWU65821.1"
                     /translation="MIYGIGTDIVEVERIQSSLDQFGDAFAKRILADSEWTDYQASQL
                     KARFLAKRFAAKEAFAKAIGTGIRGDVSFHNIAVTHDALGRPLLQLSATLQGFLKQHH
                     IYQSHISISDEKNLALAYVVLETTKSA"
     gene            8041..8829
                     /locus_tag="VNZ84_02360"
     CDS             8041..8829
                     /locus_tag="VNZ84_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HWU65822.1"
                     /translation="MSSDQFMDVSRRFSGVRRLYGEQGLASLQKAHVLVIGIGGVGSW
                     TVEALARNAVGKLTLVDLDNIAESNVNRQIHALEDNLGKAKVTAMRERITAINPQCDV
                     VEVEDFVTPDNLSKMLADSPDIVVDCMDDTKAKIALAAHCKQHKLPLVMVGSAGGKLD
                     ATLLRLADLSQVQGDRMLAKVRNQLRRDHGFPKASDHKKSARFGIMAVYSDEPVERPE
                     EACEVPQSGLTGLNCAGYGSSICVTASAGFIAAQQAIAQLLAAE"
     gene            9059..10033
                     /locus_tag="VNZ84_02365"
     CDS             9059..10033
                     /locus_tag="VNZ84_02365"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HWU65823.1"
                     /translation="MQLDVFLKKVSINHALNVLIWHIAEAGIEIAALVKQGALEGVTE
                     KMTTTNVQGETQMQLDIRSHEIAVAQLRTSAVVAAVLSEEEDDPVLFETLDAPFLVSM
                     DPLDGSSNLAINGVVGSIFSVLPNTGVATLGEQAFLQPGQDQRAAAYIMYGPATLLVL
                     TIGQGTHVFTLDHSNQVFVLTQQTVNIPEETSEFAINASNQRYWQPGVQRYIAECLQG
                     AEGPRARDFNMRWCASMVMDVHRILTRGGVFLYPRDSKQPVKAGRLRLLYEANPMSLL
                     VTQAGGESIDGLEDVLAIRPSACHQRVPVLLGSKQEIQRLREYHQSTL"
     gene            10067..11101
                     /gene="fba"
                     /locus_tag="VNZ84_02370"
     CDS             10067..11101
                     /gene="fba"
                     /locus_tag="VNZ84_02370"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883118.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWU65824.1"
                     /translation="MALISLRQLLDHAAENSYGYPAFNINNMEQILSIMKAADEVDSA
                     VILQASAGARGYAGESFLRKMVEAAIEQYPHIPVCMHQDHGTSPKICQMAIRSGFSSV
                     MMDGSLKEDHKTPATYEYNVDVTRRVVEFAHAVGVSVEGELGVLGSLETGMAGEEDGV
                     GAEGKLDESQLLTDPDEAAAFVEATKVDALAIAIGTSHGAYKFTRPPSDDTLSIERIR
                     EIHAKIPNTHLVMHGSSSVPQSLLEQIRHYGGNIKETYGVPVSQIVEGIKNGVRKVNI
                     DTDIRLAMTAAIRAHLAQHPEDFDPRQYFKEATIAAQHLCKERFEAFGSAGQASKIKV
                     VSLEKMAAIY"
     gene            11177..12007
                     /gene="tpiA"
                     /locus_tag="VNZ84_02375"
     CDS             11177..12007
                     /gene="tpiA"
                     /locus_tag="VNZ84_02375"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWU65825.1"
                     /translation="MRQKLVIGNWKLHGGLLENQGLLNRLKQELHDLPGVDTAVCLPY
                     VYLFQAQSLLQDSQIAWGAQNVSQFTEGAFTSCISAKMVAEFGCTYTIIGHSERRALK
                     LESNQVATKRMLNALHAGLTPIFCVGETLDERDGNMAELIVRNQMLNVVYGLDDEAFA
                     LAKKFNMVIAYEPVWAIGTGEHASPEQAQRMHAFIRMMIAERDREFAESIRIVYGGSM
                     APQNAGSLLSMPDIDGGLLGRAALVAEDFVEICRIANRCYLQKTAFKEPSPAVPVFEA
                     "
     gene            12004..12165
                     /locus_tag="VNZ84_02380"
     CDS             12004..12165
                     /locus_tag="VNZ84_02380"
                     /inference="COORDINATES: protein motif:HMM:NF021526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HWU65826.1"
                     /translation="MTFTFKGNKSRLPAKPCQQCGRPMTWRKAWANNWDQVKYCSERC
                     RRASKIIAA"
     gene            12162..13706
                     /locus_tag="VNZ84_02385"
     CDS             12162..13706
                     /locus_tag="VNZ84_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HWU65827.1"
                     /translation="MRHLIIILGDQLTLDNPALEGIDPSQDHIVMAEVMEESTHVWSH
                     KQRTTLFLSAMRHFAAQLQAQHLPLTYLKLDDHTYPDLPAVWHAMLQKHRPAAIKVCE
                     PGDYRVWQDLQAVANQHSTPVEVLQDTHFMCSRDQFQRWAAKYGQKNSSLRMEFFYRG
                     MRKKYNILMDGDAPMGGDWNYDHDNRQPLGKHGPQNLVPPPVFKPDAVTREVQALVEV
                     RFAEHPGSLDAFSWPVTRDEALTLLAHFVAEKLPQFGPYQDAMWQDQPYLWHSLLASA
                     MNLKLLNPREVIAAAEQAYLAGQVPLLCAEGFIRQILGWREFVRGVYWLEMPALKAAN
                     YFQHQSDLPGWFWTGDTHMQCLSQCIGQTLRTGYAHHIQRLMVIGLFSTLAELSPQQV
                     SDWFLAVYVDAVEWVELPNVIGMALYANGGRFTSKPYVASGAYIQRMSNYCSRCCYDP
                     KQKTGDRACPFTTLYWAFLIKHEGALADNPRTRLMVKHVANLSADDRAAIQAMAGQRL
                     QQLEQL"
     gene            13773..15815
                     /gene="ppk1"
                     /locus_tag="VNZ84_02390"
     CDS             13773..15815
                     /gene="ppk1"
                     /locus_tag="VNZ84_02390"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HWU65828.1"
                     /translation="MKNNTPVLLDRDLSILSFNARVLSLAQREDYPLLERLRFLCIVS
                     NNLDEFFEVRMPLQMEAYQQGVTSGPVTAASCEQVAEMAHSLVAQQYRLFNDALMPAL
                     AKENVHLVSGGVRTPEQTTWVANYFKREVLPFLMPVSLDPSHPFPQVANKSLNFIVRI
                     AVDGEEKIAIVRAPRVVPRVVQLPPSISKGEQCFVSLTSIIRANLDVLFEGASILHFS
                     QFRVTRNSDLEVDEDDVINLRTALREELAHRQYGEAVRLEVTQECDDTLAEFLLKQFN
                     LPALALYRVNGPVNLGRLIQLPDMAKGDHLKFVPFQPHWPQHIKKNISLLAQMREKDL
                     LIHQPYESFEVVIQLLEEAVKDEQVLAIRMTIYRTGADPRMLRLLQEAVRRGKEVLVV
                     VELKARFDEEANINWAEALESVGAQVVYGVVGLKTHAKMLLIMRREGKVIHRYGHLST
                     GNYNPKTTRLYTDWSMLTADANLTREMEAVFRHLTSELPIPVMKHLLVAPFTLQSSMI
                     RQLVKAQRIAKQGKPARVIAKMNALTDVPLVAALMKAAAAGVEIDLVVRGACILPVDS
                     PAVKGRIRVRSIVGRLLEHSRVFYFELDGQVQMWLSSADWMSRNMMRRVELAWPIKDP
                     AMQQRIYQESLQIYLQDNKDAWQLAEKGQYIPVAELEQDAEPVACQSLLLKQYHSM"
     gene            15824..16288
                     /locus_tag="VNZ84_02395"
     CDS             15824..16288
                     /locus_tag="VNZ84_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWU65829.1"
                     /translation="MPVKNLILWRHAEAEVLLHGASDLERALTKKGHKQAKQMAGWLK
                     KYLPKQTYVLVSPSVRTRETVAYWGEDWQEDTRLAPERPLAPLLQLLDQSPFESVMLV
                     GHQPWIGELAAHGLGMPDGQISIKKGAVWWLRLPKSGPPYKLYSVQSPDLIE"
     gene            complement(16323..16796)
                     /locus_tag="VNZ84_02400"
     CDS             complement(16323..16796)
                     /locus_tag="VNZ84_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65830.1"
                     /translation="MASKPVAKPLTANKPATNAVKTSTPTKPVVAKPAAQKPASAKPV
                     AAKPPVAKVAAKPPVAAKKVAPAPAIKVVNKTATATDKAKTSKVKMERDSFTMPKEEY
                     AQIATLKKRLEGLGKPAKKSELLRAGLKLLSAMDDAALKATMATVPTIKTGRPKK"
     gene            16940..17560
                     /locus_tag="VNZ84_02405"
     CDS             16940..17560
                     /locus_tag="VNZ84_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWU65831.1"
                     /translation="MRRVLVANTKGGCGKTTLATNLAGYLAQLGHKIVLEDIDRQQSS
                     AAWLARRPPHVATVFNAEDLGKSALQKADWVIVDSPGGLRDKKISDAVTEADLVLVPV
                     APSAFDIGATQDFLELLAEEKAVRKHKTFVGLVGMRVMPRTRSAERLLAFMDESGFAV
                     LSMLRSSQIYVTAAEEGLSIFELSNTLKDVDIAQWNPVTDWILQNS"
BASE COUNT         4334 a         4158 c         4901 g         4387 t
ORIGIN      
        1 cgctttggta tcccgggcat gccgggtggg cctgggcagg atggtggcca ggactataaa
       61 acgcaatcac tgggctccgg ttttattatc agtgcagatg gatatattct gacgaatgcg
      121 catgtagtga atgaggctga tgaagtcctg gtgaagctgt atgacaaacg tgaattcaag
      181 gcgaaaatta tcggtgccga caagcgcacg gatgtcgctt taatcaaaat tgaggcgtct
      241 ggcttaccca aggtcaccat tggtgacccg aatgcactca aggtggggga gtgggtggct
      301 gcgattggtt cgccatttgg tcttgaaaat acgatgactg cagggattgt cagtgccaag
      361 ggacgcgcct tgccacagga aaactatgtg ccttttatcc aaacggatgt ggccattaat
      421 ccgggtaatt caggtgggcc attgttcaac ctgcgcggtg aggtcgttgg tgtgaactca
      481 cagatttaca gtaaaagtgg cggttccatg gggctgtctt tcagtatccc gattgatgtc
      541 gctattgata tttccaacca attaaaaacc aatgggcata ttgcccgcgg ttggctgggg
      601 attggtatcc aagaggtgac taaagacttg gcggagtcgt tcggcttgaa gcagaccagt
      661 ggtgcactga ttgctggtgt agaaaaaaac agcccggcag acaaaggtgg cctggaagcg
      721 ggcgatatta tccttaagtt tgatggtaag ccaattgtga cttccagtga tttgcctcgc
      781 gttgttgcag cgaccaagcc cggtaaggtt gtgccggttg aaattgtgcg caaaggggca
      841 aacaagactc ttcagttagg cgtaggtgaa atgcctagcg aacagggtga ggtgcctgct
      901 gcgaataaag agccagcgaa accagaggcc aataggatag gtctgacctt gcgtgaatta
      961 acgccgcaac agaagaaaaa actcaatggc aagaatggct tgttggtgat tgaaagtagc
     1021 ggtgcagcag cgcaagcagg gatacgtcgt ggtgatgtga tcctgggttt gaacaataac
     1081 gaaagtcaga gtgttgattt gtttaacaag cagattaatg cggtcgctgc gggtaagacg
     1141 gttgcggtgt tggtgcagcg tggtgaaaat acgttatacg ttccgatcaa aatcaagtaa
     1201 ctgtattgtg tggtgatggt tgcaaaaagg caggtttacc tgcctttttg caaggaaatg
     1261 taggttgccc acgtgattgt caatggaaaa taaatcactg atattcatac atttgctgta
     1321 aaatgcaacc gatttgaaag gcgcttcaag cgcctttctt gcttgtaatt aacgcataac
     1381 agaacatgaa aaatattcgt aatttttcga ttattgccca cattgaccat ggcaaatcga
     1441 cactggcaga ccgcattatc caattgtgtg gtggcctgga ggcgcgtgag atggaagcgc
     1501 aggtgttgga tagcatggat attgaacgtg agcgtggcat taccatcaaa gcgcaaacag
     1561 ccgcattgca atacaaagcc aataatggcg aaacctactt cctcaacctg attgataccc
     1621 ccggccacgt ggactttagt tatgaagtca gccggtcact gtctgcctgt gaaggtgcat
     1681 tgttagtggt cgatgcctca caaggagttg aagcgcaaag tgtggccaac tgttacaccg
     1741 cgattgatct gggtgttgaa gtgactcccg tcttgaacaa gattgacttg ccttccgctg
     1801 acccggcgcg tgtatcgcaa gagattgaag acgttatcgg ggtcgatgcg actgatgccg
     1861 tgcgttgttc agccaaaacc ggtgaaggcg tgcgtgatgt gctggaagaa gttgtcgcca
     1921 aggtgccgcc ccccactggc gatgccgctg ccccgctcaa agcgctggtg attgacgcct
     1981 ggtttgataa ctatgttggc gtggtcatgc tggtgcgcgt ggttgatggc gtattgcgtg
     2041 ccaaagaaaa aatccgcctg atggcgacag gcgctgaata cctgtgtgag caggtgggtg
     2101 tgtttacgcc taaatccttg cagcgcccac agttgagcgc cggcgaagtc ggcttcatta
     2161 ttgccgggat caaagagttg gccgcggcca aggtcggtga tacggtcact ttggcaggta
     2221 aaccagcggc agagcctttg ccaggcttca aggaagtgaa gccgcaggtg tttgcgggcc
     2281 tgtatccggt cgagtccaac cagtttgaag ctttgcgtac cgcactggaa aaactgcgtt
     2341 taaatgactc cagcttgcag tttgagccag agaactcatc tgcactgggt tttggtttcc
     2401 gttgtggttt cctgggtctg ttgcatatgg aaatcgtaca agagcgcctg gagcgcgagt
     2461 acgatatgga cctgattacg actgcaccaa ccgtgattta tgagctgctg ctcaagagcg
     2521 gtgatgtggt gcagatcgaa aacccatcgc gcctgcctga gccttctcgg attgaagaga
     2581 tccgtgagcc tatgattgtc gtcaacttgt tgatgcctca ggattatgtg gggccggtga
     2641 tgacgttgtg caacaacaag cgtggcgtac aacgcaatat gcagtatatg ggacgtcagg
     2701 tcatgctcag ttatgagatg ccgctgaatg aagtggtgct cgacttcttt gataagctta
     2761 aatccgtgtc acgcggttat gcatccatgg attatgagtt cctggagttc cgtgcggcag
     2821 acttggtcaa gctggatatc atggtcaacg gcgaacgggt ggatgcattg agcttgatcg
     2881 tgcatcgtgc caatagtgtt taccgtggcc gcgagctggt cgcaaaaatg cgtgagctga
     2941 ttccgcgcca gatgtttgat atcgccattc aagcctcgat aggggctaac atcattgcgc
     3001 gtgaaaccgt aaaagccatg cgtaaaaacg tgctggccaa gtgttacggt ggcgatatta
     3061 cacgtaagcg caagttgctg gaaaaacaaa aagaaggtaa aaagcgtatg aagcaggtcg
     3121 gtaatgtgga gattccacaa gaagccttcc tggctatctt gcgagttgaa gataaataaa
     3181 aaacgggaac acccgctctt tacaggaagc aagcatgtat ttcgcaattt ttatgttggt
     3241 cattttactg gtgtctggtg ccatctggct gttggacatt ctggtgctac gcaaacaacg
     3301 ccaggctggc gcgctgggcg atgtgcctga tccatggtac gtggagtata gtaaaagctt
     3361 cttcccggtg atattgctgg tcttcatggt gcgctctttt gtggtcgagc ctttcaagat
     3421 tccttctggc tccatgatgc caaccttgct ggctggggat tacatcctgg tcaataaatt
     3481 tacctatggc ctgcgcgtac ctattctcaa taatgtgttt taccccgtga gcctgccaaa
     3541 acgtggcgat gtctttgtat ttcattatcc gcctgatacc tcaattgact atatcaagcg
     3601 tgtggtcggt ttgccgggcg acaaaatcca gtaccgtgac aagcaactga ccattaacgg
     3661 taaggtcatc aacctggact tcgtgcaacc atatcaatat gcgctcaatg tcaatgatga
     3721 gtctgggaat gcgcgtgcag tggatgtgac ggcgtcacgc ctgagcgagc aattagggga
     3781 tgtcaaacat gacgtactga ttcatgacat catgaaccag tacggccccg ggagcttggg
     3841 tgaccgcttg atgcagggcg aaaccattac tgtgccagaa ggtgagtatt tcgccatggg
     3901 cgataaccgg gacaacagct cagacagtcg tgtctggggc tttgtggcag aaaaacacct
     3961 ggtgggtaaa gcatttttta tctggttcaa ctttgaccaa tggcgccgta tcgggcaaag
     4021 tatcggttaa tgcacggtta gcgccgtgtt cctgggcatc cttttgaggg ctagcatatg
     4081 aaattctcaa agacttctac ccaacacggt attggcttga tcggcttttt gctggtatta
     4141 acggcgtttg gcatattggg tatggtcgcg ttaaaagcgc tgccatccta tatggaattt
     4201 ttctctattc agtctaccgt caaccgcatc gcgcatgatc ccatgctgca aaatgacagt
     4261 gatatccgca agtcgtttga ccgccagatg gaagtggaca gcattcacga agtgacggga
     4321 cgcgatttgg aaatcagtgg tggcgtggtt gccatgcgct accagaaaaa ggtgccactg
     4381 accgacgcaa tgacgctgct gattgatttt gacgcaacca gcaaaccata gtgatgcagg
     4441 cttgacgata acgtaatgaa tgcactcccg gtattacaaa ccagaatcag ctaccagttt
     4501 gcgcagttag aactgttgca acaagcactt acacaccgca gcttctctgc gagtaataat
     4561 gaacggttgg aatttctggg cgatagcgtg ctcaatttca ttatcgctca cctgttgttt
     4621 aatttattcc caaatttgcc tgaaggcgat ttaagccgtt tacgcgccaa gttggtcaaa
     4681 gaatccagtt tggccgaaat cgcaacagat ttgcatttgg gcgatgcgct caaattgggt
     4741 gagggcgaac tgaaaagtgc aggctggcgg cgtccttcca ttttggccga tgcacttgag
     4801 gcaattgtag gtgcagtgta cctggacggt ggctttgatg cggcacagca agtggttgcc
     4861 ttattgtatc gcgacaagct cagcaccata gatcccaaag ccatcgacaa agacgccaaa
     4921 tcacagttgc aggaatacct gcaaagtaaa aaaatggagt tacctgaata tcaggtggtg
     4981 tccatagagg gggaggcaca tgcgcagacc tttaccgtgc aatgttttat taaaaagctc
     5041 aaactgacta ccaccggcgt gggcactagc cgccgtgtgg ccgagcaaca agcggccaaa
     5101 ttggccatgg acaatattca atcatgacag aacttacccc cgctgcagac gatttccgtt
     5161 gtggcaccgt cgctatcgtt ggccgtccca atgtcggcaa gtcgaccttg cttaaccata
     5221 ttttgggcat gaagctggcg atcacttcgc gtaaagcaca gaccacgcgt caccgtttgc
     5281 tagggataca taccacggaa gacacgcagt ttctgtttgt cgatacgcct ggattccagc
     5341 aaaagcatct caatgcctta aacaagtcgt tgaacaaaac cgtcacccag gtgctgactg
     5401 aagtggatgt cgtgttattc gtgatcgagc ccatgaagct gggcgaggca gacgaactgg
     5461 tgctgaaatt gttgcctaaa aacaaaccgg tcttgctggt tgtgaacaag gctgatttga
     5521 tgggtgacaa aggcaatttg ctgcctttga tccaggactt tgacctgctg tttgatttcg
     5581 ccggcattat cccggtcagt gctaaaaaga atctttacct ggatgagtta ttggctgctg
     5641 tacgcgagca cttgccgcag cagccagcca tttacgctga agatgagctg actgacaaaa
     5701 acgaacgctt cctggcagct gaattggtgc gtgaaaaagt cttccgcttg cttggagatg
     5761 aagtgcctta ttcagtcacg gtagaaattg aaaagtttga gacggtcaaa aatctgcgcc
     5821 gtatttttgc ggccattatt gttgataaag atagccagaa accgatgatt attggcaagg
     5881 gtggagagaa actcaagcag atttccactg aagcgcgcca agacatggaa aaattgttcg
     5941 gttccaaggt atacctcgaa acctgggtta aggtgaaagg tggctgggca gatgatgcgc
     6001 gtgcgctaaa aagcctgggc ctctaataag cctaacgcta ctggcatcac atatggcagt
     6061 gcactctcct gactctggta gccataaaca gtccctgcaa ccagttttta ttcttcatac
     6121 ttaccctttt aaagaaacca gcctggtcgt tgaaatgttc agccgcgacc tgggccgtat
     6181 tgcggcagtg gcaaagggcg cacgacggcc tttgtctgcg atgcgaggca tcttgcagtc
     6241 ttttcagcag ctggctggca cctggtcagg caagaatgaa ctcaaaaccc tgcatggcct
     6301 ggagtggatg acagggctga cattgctaag gggcgacgcg ctcatgtgcg ggttttacat
     6361 gaacgagcta ctattgcgcc tgctgccgcg ggatgatgcc catacccagt tattcgagta
     6421 ttacgcgcaa accatacagc tactttcgac cctgcagcaa gatgctggta gtggtcagct
     6481 ggccgagatg atgcgccggt ttgaactcaa gatgttgcag gaactaggct atgctgtgcc
     6541 actgacacat gatgaacatg gcgaagtgat acatgcggat gaaatgtacc gctttgaagc
     6601 tgattatggc gcatgcgcac cgtctgccac taaaaatgga ttgctggtgc agggcgcaac
     6661 cttgcttgaa atggctcgcg gtaattacag tagcgccacc acacagtcac aaagcaaaca
     6721 gctcatgcgt taccttctac agcactacct gggtgaaaaa cctttgcata cacggcagtt
     6781 gctggtcgat ttacaaaact tctgatcgtg accgcctgga agggcataac gatgccaggg
     6841 cgattccgtt tataatgaag ccaaagaaag tctgtggctg ataaatcgaa agtaactatg
     6901 ttaaaactag gcgtcaatat tgatcacgtg gcgaccattc gccaggcgcg tggcaccaga
     6961 tatccaagtg tggtgcaggc cgcattgcgt gccgagcagt cgggtgcaga tagcataacc
     7021 ctgcatttgc gcgaagaccg tcgccatatg caggatgctg atatttttgc cttgcggccc
     7081 ttgctgcaaa ccaaaatgaa tctggaatgt gcggtgacca gcgagatgat agatattgcc
     7141 atccaggtca atccgcaaga tgtctgtctg gtgccagagc ggcgtgaaga gcgtacgacc
     7201 gagggcgggc tggatgtgct gacacattac gccaaagttg agcatgcagt gaaaaagctg
     7261 tctgatcaag gcatacgcgt gtctctgttt attgcgcctg acctggcgca gattgatgct
     7321 gccgccaaaa tgggtgcgcc tgtgattgaa atccatacgg gcacctttgc cgatgctgag
     7381 acagaaaagc aacagcaaga ggaactggaa aaaattcagg tggcattgca gcacgcgaaa
     7441 gctgcggacc tggtcgtcaa tgccggtcac ggattgcatt atcacaatgt gcaccatatc
     7501 gcgcgattgc ctggatttga ggagcttaat atcggtcatg cgattgtggc gcatgcctta
     7561 tttgtggggt gggatagtgc agtgcgtgag atgaaagcac tcatgaaaga attttcacca
     7621 agatgatgca gtcgcttaat catttggagt ctcaatgatt tacggtattg gcacagatat
     7681 cgtcgaggtc gaacggatcc agtcttcact cgaccaattc ggtgatgcct ttgccaagcg
     7741 tatcctggca gattcagaat ggaccgatta ccaagccagc caattgaagg cacggttcct
     7801 ggccaagcgc tttgccgcca aagaggcatt tgccaaagcc attgggacgg gtatccgtgg
     7861 agacgtcagt tttcataata tcgccgtcac gcatgatgct ctggggcggc ctttactgca
     7921 attatctgcc accttacaag gctttcttaa gcaacatcat atttaccagt cccatattag
     7981 tatcagtgat gaaaaaaatc tggcgctggc ttatgtggtg ctggagacaa ctaaaagcgc
     8041 atgagtagtg atcagttcat ggatgtctca cgccgttttt ccggcgtccg tcgtctgtat
     8101 ggcgagcaag gcctggctag tttgcaaaaa gcccatgtgc tggtgatcgg catcggtggt
     8161 gtagggtcat ggactgtgga ggcactggcg cgcaatgcag tcggcaaatt aacactggtt
     8221 gatctggata atattgcgga atcaaacgtc aatcgccaga ttcatgcgct ggaagacaac
     8281 cttggtaaag ctaaagtcac cgccatgcgt gagcgaatta cagcgattaa tccgcagtgt
     8341 gacgtggttg aagttgaaga tttcgtcacg ccggataatt tgtcaaaaat gctggcggac
     8401 tcaccggata tcgtggtcga ctgcatggat gacacaaaag ccaaaattgc ccttgccgcc
     8461 cattgcaagc agcataaact gccattggtc atggtaggca gtgcgggtgg taagctggat
     8521 gcgacacttt tacgcctggc ggatttgtca caggtacaag gcgaccgcat gctggcaaag
     8581 gtacgcaacc agttgcgccg tgaccacggg tttcccaagg cgtctgatca caagaagtca
     8641 gccagattcg gcatcatggc tgtgtactcg gatgagccag ttgaacgacc tgaagaagcc
     8701 tgtgaggtgc cgcagtcggg gctcacaggc ctcaattgcg cgggatatgg ctccagtatt
     8761 tgtgtcaccg ccagcgcagg attcatcgcg gcgcaacagg ccatcgcgca gttattggcg
     8821 gcagaataac gccaactttt ttcaaaaaac atttttattg cattcatttc tgccatcata
     8881 aggttgaaca atgtcgtcag tatggcgtct accttaaaaa ggtcaggctt cacgtgactt
     8941 tgtctctgca ggtaaataca gccattttca gcctgtgatc atgattttaa gtcattgcca
     9001 tatcagatag agtaatgaca taaagcccaa gtttgcttca tccatcaaag gtattgccat
     9061 gcaattggac gtgtttttaa agaaggtttc tatcaatcat gccttgaacg tgctgatctg
     9121 gcatattgcc gaagcgggca ttgagattgc ggcactggtg aagcagggag cattggaagg
     9181 ggttaccgaa aaaatgacca ctaccaatgt ccagggggaa acccagatgc aactggatat
     9241 ccgtagtcac gagattgcag tggcccagtt gcggacatcg gctgtcgtgg ctgcagtatt
     9301 gtctgaagaa gaggatgatc ctgtgttgtt tgaaacgctg gatgcgcctt ttctggtcag
     9361 catggatcct ttggatggtt cttccaattt agcgattaat ggcgtggtcg gcagtatctt
     9421 ctcggtattg cccaatacgg gcgtggcaac cctgggcgag caggcttttt tacaaccggg
     9481 tcaggatcag cgggcagccg cgtatatcat gtacgggcca gcgactttgc tggtattgac
     9541 gattggccaa ggcacgcatg tgtttacatt ggaccacagc aaccaggtgt ttgtattgac
     9601 gcagcaaacc gtcaatattc ctgaggagac ctcagaattt gccatcaacg ccagtaatca
     9661 gcgctattgg caacctggcg tgcagcggta tattgcagaa tgtctgcaag gtgccgaagg
     9721 accgcgtgcg cgtgacttta atatgcgctg gtgtgccagt atggtgatgg atgtgcatcg
     9781 tattttaacg cgtggcggtg tgttcctgta cccccgcgat agcaaacaac ccgtgaaggc
     9841 cggccgctta aggctgttat atgaagccaa tcccatgagc ctgttagtaa cgcaggccgg
     9901 cggggaaagt atagacgggc tggaggatgt tctggcgata cgacctagcg cctgccatca
     9961 gcgcgtaccg gtgctgctgg gctcaaagca ggaaattcag cgcttgcggg aatatcatca
    10021 gtcaaccctt tagcatcagc aagccttacc ttagaaggag tgttacatgg cattgatctc
    10081 gctcaggcaa ttactcgacc acgccgcgga aaatagttat ggttatccgg cattcaatat
    10141 caataatatg gagcagatac tctccatcat gaaggccgca gacgaagtgg atagtgcggt
    10201 gattttgcag gcgtcagccg gggcaagagg ctacgccggg gagtcattcc tgcgcaagat
    10261 ggtagaagct gccattgagc aatatccgca tatccccgtg tgtatgcacc aggatcatgg
    10321 gacctcaccc aagatttgcc agatggcgat ccgtagcggg ttttccagtg tgatgatgga
    10381 tggctcttta aaagaggatc ataaaacgcc tgccacttac gagtacaacg tcgatgtcac
    10441 gcgccgtgtc gtcgagtttg cacatgcggt gggggtttcg gtagaaggtg agcttggtgt
    10501 gctcggctca ctggaaaccg gcatggctgg tgaagaggat ggtgtgggtg cagaaggtaa
    10561 actggatgaa tcgcaattgt taaccgaccc ggatgaagca gccgcttttg tagaggcgac
    10621 caaggtcgat gccctggcga ttgcgattgg taccagtcat ggcgcgtata aatttacccg
    10681 tccgccttca gatgatacgc tgtcgattga acgtatccgt gaaattcatg ccaaaattcc
    10741 taacacgcat ctggtgatgc atggctcttc cagcgtgccg cagtccctgc tggaacaaat
    10801 ccggcactat ggcggcaata tcaaggaaac ctatggcgtg ccggtctctc aaattgtgga
    10861 gggcattaaa aatggggtgc gtaaggtcaa tattgatacc gatatccgcc tggccatgac
    10921 tgctgcgatc cgtgcccatc tggcgcagca tccagaggat tttgatccgc gacaatattt
    10981 taaagaagcg accattgccg cgcagcattt atgtaaagaa cgctttgagg cctttggtag
    11041 tgccggacaa gcgagtaaga taaaggtagt ctcactggaa aaaatggccg ctatttatta
    11101 agcgccaatt cattaccatc ttagacacag cctaaaacaa caataatcga gggggcatga
    11161 gtaaaggaat ttcatcatgc gacaaaagtt agtcattggt aactggaagt tgcacggcgg
    11221 tttgcttgag aaccaggggt tgttgaaccg cctcaagcaa gaattgcacg acctgccagg
    11281 tgtggatact gccgtgtgtt taccttacgt gtacctgttc caggcgcaat ccttgctgca
    11341 agattcccag attgcctggg gcgcacaaaa cgtcagccag tttaccgaag gcgcgttcac
    11401 ctcctgtatt tcggccaaaa tggtcgccga atttggctgc acctatacca ttatcgggca
    11461 ctccgagcgc cgggccctta aactcgaaag taatcaggtg gcgaccaagc gcatgctcaa
    11521 tgcgctgcat gcagggttga cgcccatttt ttgcgtgggt gaaaccttgg acgagcgcga
    11581 tggcaatatg gccgaactca tcgtccgtaa ccagatgctg aatgtggtct atggcctgga
    11641 tgatgaggcg tttgcgctgg ccaaaaaatt taatatggtg atcgcctatg aaccggtatg
    11701 ggcgattggc acaggcgagc atgccagccc ggagcaggcg caacgcatgc acgcctttat
    11761 ccgcatgatg atcgccgaac gtgaccgcga gtttgccgaa agcatccgta ttgtttacgg
    11821 tggcagtatg gcgccccaaa acgccggtag cttactcagc atgccggata ttgatggtgg
    11881 tttgctgggc cgtgcggccc tagttgcaga agattttgtc gaaatttgca gaattgctaa
    11941 ccgttgctac ctgcaaaaaa cagcgtttaa ggaaccctcg cccgccgtac cagtctttga
    12001 ggcatgacat tcacattcaa aggcaataaa agccgcttgc cggcaaaacc ctgtcagcaa
    12061 tgcggccgac ccatgacatg gcgaaaggca tgggccaaca attgggatca ggtgaagtat
    12121 tgttctgaac gctgtcgccg agcctctaaa atcatcgcag catgaggcac ctcattatca
    12181 tcctcggcga ccagctcacg ctggataatc cggccttgga agggatcgat cccagccaag
    12241 atcatattgt gatggcagaa gtgatggaag agtccactca tgtctggtca cacaagcagc
    12301 gcactaccct ctttctctca gccatgcggc actttgccgc acaattgcaa gcgcaacacc
    12361 tgccgcttac ctatctcaag ctggatgacc acacctatcc agatttgcca gctgtgtggc
    12421 atgccatgct acaaaaacat cgtcctgcgg ccatcaaggt ctgtgagcca ggtgactatc
    12481 gggtctggca ggatctgcaa gcggttgcaa accagcattc aacgcctgta gaggtgctgc
    12541 aagatacgca ttttatgtgc agcagggatc agttccagcg gtgggcagcc aaatacggcc
    12601 agaaaaatag ctctttacgc atggaattct tttaccgcgg catgcgcaaa aaatacaaca
    12661 tcctcatgga cggggatgcg cccatgggtg gtgactggaa ttacgatcat gacaaccgcc
    12721 agcctctggg taaacacggt ccacaaaact tggtgccgcc accggttttt aagccggatg
    12781 cagtgacacg agaggtgcag gcgctggttg aggtgcgctt tgcagagcat ccgggctcac
    12841 tagatgcctt ttcctggccg gtgacacgtg atgaggcgct cactttactc gcacattttg
    12901 tcgcagaaaa gttgccacag tttggcccat atcaagacgc aatgtggcaa gaccagccat
    12961 atttatggca ttcattgctg gcgtctgcca tgaacctcaa attgctcaat ccgagagaag
    13021 tcattgccgc tgcggaacag gcctatcttg ctgggcaggt gccgctcctg tgtgcagaag
    13081 gctttatccg ccagatattg ggctggcgtg aatttgtgcg tggtgtatat tggctggaga
    13141 tgccagcact caaggcggca aactatttcc agcatcaaag tgatctgcct ggatggtttt
    13201 ggacagggga cacgcatatg caatgtttgt cgcagtgtat cggccagact ttgcgcacag
    13261 gctatgccca tcatatccag cgcctgatgg tcataggctt gttctctacc ttggctgagt
    13321 tgtccccgca gcaagtgtct gactggtttc tggcggtata tgtcgacgca gtagagtggg
    13381 tggagttgcc aaatgtcatc ggcatggccc tatatgccaa tggtggccgc tttaccagca
    13441 agccgtacgt cgccagtggg gcttatatcc agcgcatgag caattattgc agtcgttgct
    13501 gctatgaccc caaacagaaa actggtgatc gcgcctgtcc attcaccact ttgtactggg
    13561 cgtttttgat aaaacatgaa ggcgcgctgg ctgataatcc gcgtacccgc ctcatggtga
    13621 aacatgtcgc gaatctttca gcagatgacc gcgcggcaat tcaggccatg gccgggcaaa
    13681 gattacagca actggagcaa ttgtaatgcg tgcgtgtgca atttcacaaa actgtcacaa
    13741 tagacgccag ttcagtcgat acaataatcg ccatgaaaaa taacacgcct gtcctgcttg
    13801 atagagacct cagcattctc agttttaacg cacgggtatt gtccctggcg caacgagaag
    13861 actacccgct actggaacgc ttgcgcttct tatgtatcgt gtccaataac ctggatgagt
    13921 tctttgaggt gcgcatgcca ctgcaaatgg aggcctatca gcaaggcgtg accagcggtc
    13981 cggtgacagc agccagttgc gaacaggtcg ccgagatggc gcatagcctg gtcgcgcagc
    14041 aataccgttt gtttaatgat gcgctgatgc ctgctctggc caaggaaaat gtgcatcttg
    14101 tttctggcgg cgtgcgcacc ccagaacaaa cgacttgggt agctaactat ttcaaacgtg
    14161 aagtcctgcc ttttttgatg ccggtctcgc tggacccgtc gcatcctttc ccacaggtgg
    14221 caaataaatc actcaacttt attgtgcgga ttgccgtcga cggcgaggag aagattgcga
    14281 ttgtacgggc cccgcgcgta gtgccgcgtg tggtgcaatt acctccctct atcagcaagg
    14341 gcgagcaatg cttcgtgtcg ctcaccagta ttatccgcgc caacctggat gtgttgtttg
    14401 aaggcgcaag catcctgcat ttctcacagt tccgtgtgac acgtaacagc gacctggaag
    14461 tcgatgaaga cgacgtgatc aacctgcgta ccgcgttgcg cgaagagctg gcccaccgcc
    14521 aatatggtga ggccgtgcgc ctggaagtta cacaagaatg cgatgacacg ctggctgagt
    14581 tcctgctcaa gcagttcaat ctgccagcgt tggcgctcta ccgtgtcaat ggcccggtga
    14641 acttgggccg cttgatccag ttgccggata tggccaaagg cgaccacctc aagtttgtgc
    14701 ctttccagcc acattggccg cagcacatta aaaagaatat ctccctgctg gcgcagatgc
    14761 gtgaaaagga tttgttgatt caccagccct atgaaagctt tgaggtcgtt atccagttgc
    14821 tggaagaagc cgtcaaagat gagcaggtgc tggccatccg catgaccatt tatcgtaccg
    14881 gggctgatcc acgcatgttg cggttactgc aggaagccgt ccgccgtggt aaggaagtgc
    14941 tggtggtggt ggaactcaag gcgcgttttg acgaagaggc caacattaac tgggccgaag
    15001 cgctggaatc tgttggcgcc caggtggttt atggtgtggt cggattgaaa acacatgcca
    15061 agatgctgct gatcatgcgc cgtgaaggca aggtcattca ccgttatggc catctttcca
    15121 cggggaacta caatcccaaa acgacccggt tgtataccga ttggagcatg ctcactgccg
    15181 atgccaacct gacacgtgaa atggaagcgg ttttccgtca cttgaccagc gagttgccta
    15241 tcccggtcat gaagcattta ctggttgcac catttacttt gcaaagcagc atgatccggc
    15301 agctggtcaa ggcgcaacgc attgccaaac aaggtaaacc agcccgggtg attgccaaaa
    15361 tgaacgcctt aaccgacgtg cctttggtcg cggctttgat gaaagccgcc gctgcgggtg
    15421 ttgagattga tctggtcgtc cgcggcgcct gcattttgcc ggtggactcg cctgcagtaa
    15481 aagggcgtat ccgtgtacgt tcgattgtcg ggcgcttgct ggaacattct cgcgtgtttt
    15541 actttgagct ggatggccag gtgcagatgt ggctttcgag cgcagactgg atgagccgca
    15601 atatgatgcg ccgtgtagag ctggcctggc cgattaaaga cccggcgatg caacaacgga
    15661 tttaccagga aagtttgcag atttatctac aagataacaa ggatgcctgg caattagctg
    15721 aaaaaggcca atatattcct gttgccgagc tggagcagga cgctgagccg gtggcttgcc
    15781 agtcactatt gttaaaacag tatcactcta tgtaatttct gtcatgcctg ttaaaaatct
    15841 gattttatgg cgtcatgccg aggcagaagt gttattgcat ggggcctcag accttgagcg
    15901 cgcgctcacc aagaaaggcc acaagcaagc caaacagatg gcgggctggc tcaaaaaata
    15961 tttgcctaaa caaacttatg tgctggtgag tccgtcggta cgcacacgag aaactgtggc
    16021 ttattgggga gaagactggc aggaagatac ccgtttggcg ccagagcgac ctttggcacc
    16081 tctgttgcaa ctgttggatc agagtccgtt tgagtctgtg atgttggtag ggcaccagcc
    16141 ctggataggc gaattggccg cgcatggcct ggggatgccg gatgggcaga tcagtatcaa
    16201 gaaaggcgct gtttggtggt tgcgcttgcc aaagtcaggg ccaccctaca agttatatag
    16261 tgtgcaaagc cctgatttga tcgagtaatg tgccagcgct aaacctaccg gcgagacggc
    16321 tgttatttct tggggcgacc tgttttaatc gtaggcactg tcgccatggt tgcttttaag
    16381 gctgcatcat ccatggcgga tagcaacttc aagcctgctc ttaacagttc gcttttcttg
    16441 gcaggtttgc ctaggccttc gaggcgtttt ttcaacgtgg caatctgcgc atactcttct
    16501 ttaggcatgg tgaagctgtc acgctccatt ttcactttgc tggttttagc tttatctgtt
    16561 gccgtagctg ttttattgac caccttgatg gctggtgcgg gagccacttt ttttgccgcg
    16621 acaggtggct tcgcagctac ttttgcgacc ggcggttttg cggctacggg cttcgctgac
    16681 gctggttttt gcgcagcagg tttggctaca acgggtttgg ttggcgtgct tgttttgacc
    16741 gcgttagttg ctggcttgtt ggctgttaaa ggtttggcta ccggtttgct ggccatgaat
    16801 ctctcctgga ttgatgttgt gaaaacagtt tataccgttt tttgttgacg tcaattgtca
    16861 taaaaatttc atattcttgt ggtgttatag aggttgtttt tcatgacaaa attcaacacc
    16921 ttcaatcagg gagtatgaga tgcgcagggt attggtggca aataccaaag gaggctgcgg
    16981 caaaaccact ctggccacga atcttgctgg ctatctggca caactgggtc acaaaatagt
    17041 gttggaagac attgataggc agcaatcctc tgcagcttgg ctggcacgcc gtccaccaca
    17101 tgtggcaaca gtcttcaatg ccgaagacct gggtaaatca gcgttgcaaa aggctgactg
    17161 ggtcattgtt gattctcctg gtggtttgcg cgacaagaaa atctcagatg ccgtgaccga
    17221 ggctgatttg gtgttagtgc ccgtggcgcc ttctgcgttt gatattggcg ccacgcaaga
    17281 ttttttagag ttactggctg aagagaaggc cgtgcgcaag cataaaactt ttgttggcct
    17341 ggtcggcatg cgtgtcatgc ctcgcacgcg ttcagcggag cgcctgttgg catttatgga
    17401 tgaatccggt tttgccgtac tttccatgtt gcgctcctca caaatttatg tgaccgctgc
    17461 cgaagaaggc ctcagtattt ttgaattgtc gaatacgtta aaagacgtcg acattgccca
    17521 gtggaatccg gtcaccgact ggattttaca aaacagttaa atcaagcctg ttgcaacagg
    17581 tggtaagcag aaagtcaaag ggagtcctat gaaagacaaa gtgaatccgg tatatctgga
    17641 aaaagtgaaa caattaagct cggatgaagc cgagcgtatt ttgtcacgca tggggggtaa
    17701 actacccaaa cggtttatta aagaaaaact gacccaggaa gaagccttgg ctttgcagtt
    17761 ggaaattgag gacgagcagt
//
LOCUS       DATMSK010000071         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_53477,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000071 DATMSK010000000
VERSION     DATMSK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53477"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VNZ84_02410"
     CDS             complement(<1..540)
                     /locus_tag="VNZ84_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWU65832.1"
                     /translation="MSKLLIVESPSKAKTLKKYLGGDFEVLASYGHVRDLIPKNGAVD
                     PAQQFAMKYDIIDRNSKHVDAIAKAVKSADSIYLATDPDREGEAISWHIAEILAEKKL
                     VKNKLIKRVEFNEITQTAVKHAIDHPRDISMDLVNAQQARRALDYLVGFNLSPLLWKK
                     IRRGLSAGRVQSPALRLIVE"
     gene            complement(651..1592)
                     /gene="ttcA"
                     /locus_tag="VNZ84_02415"
     CDS             complement(651..1592)
                     /gene="ttcA"
                     /locus_tag="VNZ84_02415"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HWU65833.1"
                     /translation="MKIKHYPDDVSQNFLKLRNQLISATGKAIGDYNMIEDGDTVLVC
                     MSGGKDSHAMLMILLALQERAPINFKLIAMNLDQKQPGFPADVLPAYLDKLGVEYRIV
                     EADTYSIVKEKIPEGKTTCSLCSRLRRGIIYTTAQQLGANKIALGHHRDDIVQTLFLN
                     MFYGAKMKAMPPKLATNRGEFMVIRPLAYCAEKDIASYARGMQFPIIPCDLCGSQENL
                     QRQKVKDMLNNWEREQPGRVNNIFRAIGNVEPSHLADFDLYDFKHLSQAKEEDEDPMF
                     DHEKFAANDMALTIATQDDKRIEFARKPFPVTVTTEE"
     gene            complement(1657..1980)
                     /locus_tag="VNZ84_02420"
     CDS             complement(1657..1980)
                     /locus_tag="VNZ84_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65834.1"
                     /translation="MKTRSQIITIHALAPQKPPLGTTCNGCGICSAAEPCPVSLALLW
                     PHQTPCKALLWNAEEQRYLCGMVSQPARFLRWLPGRLNNSASRLFRRWIAADTVCDAD
                     VTLLE"
     gene            complement(1997..2749)
                     /locus_tag="VNZ84_02425"
     CDS             complement(1997..2749)
                     /locus_tag="VNZ84_02425"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HWU65835.1"
                     /translation="MNSHSVSKVVLITGGAKRVGAAITRELHRQGANILLHYHTSATD
                     AQTLQAELNQARADSVATAQANLLETAALPQLIASALQQFGRLDALVNNASSYYPTEL
                     GQINADQWEELMGSNLKAPLFLSQAAASALRASQGCIVNITDMHIERPKKHYIVYSAA
                     KAGLVSLTKSLAQELAPEVRVNAVAPGPVLWPDDNQEFDEGYRQQVVNQTLLKRTGEP
                     ADIAKAVKFLIYDAPFITGHVLAVDGGRNLSL"
     gene            2792..>3950
                     /locus_tag="VNZ84_02430"
     CDS             2792..>3950
                     /locus_tag="VNZ84_02430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWU65836.1"
                     /translation="MTIQPNSSRLPALDAAAQQHCEKLLAHIQQRIQQQNGWISFAEY
                     MQMALYTPHLGYYSGEANKFGRGGDFVTAPEISPLFAQALANQVAQVLDQVSGDVLEL
                     GAGTGRLAAGLLLRLDELGQLPQHYYILEVSANLRQRQQEYLQSILPADLFSRMQWLD
                     TLPETLAGVVVGNEVLDAIPVHVLEWRAGQWWERGVGFDQQLVWQTRPLQDLSLVAHI
                     DTRLLPEGYITEVCPAAQGLIASLANMLTRGLVLMPDYGFAASEYYHPQRMQGTLMCH
                     YQHYAHDDPLIYPGLQDITAHVDFTAMAEAALAHGLDCAGYTNQAQFLINCGILQLLQ
                     QVPPEESARYLPMVAAVQKLLSPAEMGELFKVLALSKGLTLPLVGFLQGDKR"
BASE COUNT          847 a          940 c         1114 g         1049 t
ORIGIN      
        1 ctccacaatc aggcgcaagg ctgggctttg tacacggcca gcagataaac cgcggcggat
       61 ttttttccac aataaagggg ataaattgaa accgaccaga taatccaatg cgcggcgggc
      121 ttgttgagca ttgaccaagt ccatggagat atcgcgtgga tggtcaatcg cgtgtttcac
      181 cgcggtttgc gtaatttcgt tgaactctac gcgtttgatc agcttgtttt tgaccagctt
      241 cttttccgcc aggatttcgg caatatgcca cgagatggct tccccttcgc ggtccgggtc
      301 agttgccagg taaatgctgt ctgcactctt gacggctttg gcgatggcat cgacatgctt
      361 gctgttgcgg tcgatgatgt cgtatttcat ggcaaactgc tgtgccgggt ctaccgcgcc
      421 gtttttcggg atcaggtcgc gtacatgccc gtaagaggcc aagacttcaa aatcaccccc
      481 caagtatttc ttgagggttt tggccttgga gggagattca acgatcagca gttttgacat
      541 tgcgcggtct gtgtaagagt tggctagata atagcaagcg ttttaacgac tagacaatat
      601 atcggtgtgg aaagtctgta attaaagcct ttccgcagca aaacattgtg ttattcctca
      661 gttgtgacgg tcaccgggaa tggtttgcgc gcaaattcaa tgcgcttgtc atcctgcgtg
      721 gcaatggtca gcgccatatc gtttgcggca aatttctcat ggtcaaacat tgggtcttcg
      781 tcctcctctt ttgcctgact caagtgttta aagtcataca ggtcaaagtc ggccagatgc
      841 gaaggttcca cattgccgat ggcgcggaaa atgttattga cccggcccgg ttgttcgcgt
      901 tcccagttgt tcagcatatc tttaactttt tggcgttgca gattttcctg gctgccgcac
      961 aagtcgcatg gaatgatggg gaactgcatg ccgcgggcat aagaggcaat gtccttttcg
     1021 gcacagtagg cgagtggacg gatcaccatg aattcaccgc ggttggtcgc cagcttgggt
     1081 ggcatggctt tcatttttgc gccgtaaaac atattcagga acaaggtttg tacgatatcg
     1141 tcacggtggt ggcccaaggc aatcttgttc gctccgagtt gctgcgcagt cgtatagata
     1201 atgccacggc gcaagcggga gcacaggctg caagtggttt taccttcagg aatcttttct
     1261 ttcacgatcg aatacgtatc cgcctcgaca atgcggtatt ccacacctag cttatccagg
     1321 taagcgggca aaacatctgc cgggaagcca ggttgtttct ggtccaagtt catggcgatc
     1381 aatttaaaat tgattggggc acgctcttgc aaggccagta agatcatcag catggcatgg
     1441 ctatccttgc cgccgctcat gcacaccagc acggtgtcgc cgtcttcaat catgttgtaa
     1501 tcgccaatag cttttccagt cgcgctaatc aactggttgc gcaatttcag gaagttttgg
     1561 ctgacgtcgt ctgggtagtg tttgatcttc atagccgcgc attatagcgg aaagcaagct
     1621 aaagtcacgc taagtctttg attattcaaa ttgattttat tccagcaatg tcacgtcggc
     1681 atcgcagacc gtatctgcgg caatccagcg cctgaacagg cgactggccg agttgtttaa
     1741 tcgcccgggt agccagcgta gaaaccgagc gggctggctg accatgccgc acaggtaacg
     1801 ttgttcttcg gcgttccaga gcaaagcttt gcaaggtgtc tgatgtggcc agagtaatgc
     1861 caaactcact gggcagggct ctgctgcaga acatatgccg cagccgttac atgttgtgcc
     1921 cagcggtggc ttttgtgggg cgagtgcgtg tatggtgatg atttgcgaac gggttttcat
     1981 cacctgaatg ttcgttttat aaactcaggt tgcggccacc atccaccgcc agcacatggc
     2041 cggtaatgaa tggggcatcg taaatcagga atttcactgc ttttgcaata tccgctggtt
     2101 ctccggtgcg cttgagtagt gtttgattga ccacttgctg acggtaacct tcatcaaact
     2161 cctggttgtc atccggccat aacaccggtc ctggtgccac ggcgttcacc ctgacttctg
     2221 gggccagttc ttgtgccagt gatttggtca ggctgaccaa gccagccttg gcggcgctgt
     2281 acacaatgta gtgtttttta gggcgttcaa tatgcatgtc agtgatgttg acgatgcaac
     2341 cctggctggc tcgcaaggcg gatgcggcgg cttggcttag aaacaaaggg gctttcaagt
     2401 tgctacccat cagctcttcc cattgatctg cattgatctg gccgagttcg gtggggtagt
     2461 agctggatgc attgttgacc aaggcatcca agcggccaaa ttgttgtagt gcagacgcga
     2521 tcaattgggg caaggcagct gtttcgagca ggttagcttg tgctgtggct acactatcgg
     2581 cgcgcgcctg gtttaactca gcttgcaaag tctgcgcatc agtagcgctg gtatggtagt
     2641 gcaagaggat attcgcacct tggcgatgga gttcacgcgt gatggcggca ccgacgcgtt
     2701 tggccccgcc ggtgatcagc acaactttag atactgaatg ggaattcacg atgctctctt
     2761 tattctggac tgctcacatt ataatggctg aatgactatc caaccgaatt cttcccgctt
     2821 gccagcactg gatgccgcgg cacaacaaca ctgcgagaaa ctgctcgcgc atattcaaca
     2881 gcgcatacag cagcaaaatg gctggatatc gtttgccgag tatatgcaga tggctttgta
     2941 taccccgcac cttggatatt acagcggcga agctaataag ttcgggcgcg gtggcgattt
     3001 tgtcactgcg cctgaaattt cccctttgtt tgcgcaggcc ttggccaacc aggtggcgca
     3061 ggtgctggat caggtatccg gtgatgtcct tgagttgggt gctggtaccg gcaggctggc
     3121 ggccgggtta ttgttgaggc tagacgagtt ggggcaattg ccgcagcact attatattct
     3181 tgaggtcagt gctaacttgc ggcagcggca gcaagaatat ttgcaaagca tcttgcccgc
     3241 tgatttgttt tctcgtatgc aatggctaga tacgctgcca gagaccttgg cgggcgttgt
     3301 tgttggtaat gaggtgttgg atgccattcc tgtgcacgtg ctggaatggc gggccgggca
     3361 gtggtgggag cgaggtgtcg ggttcgacca gcaactggta tggcaaacac gacctttgca
     3421 agatttgtcg ctagtcgcac acattgatac gcgacttctg ccagagggct atatcactga
     3481 agtgtgcccg gcagcacaag ggctgattgc cagtctggct aacatgttga cgcgtggcct
     3541 ggtactaatg cctgattatg ggtttgcagc aagtgagtac taccatccac agcgcatgca
     3601 ggggacattg atgtgtcatt accagcatta tgcacatgat gatccattga tttaccccgg
     3661 tctgcaggat attactgcac atgtggactt caccgcgatg gctgaagccg cattggcaca
     3721 tgggctggat tgtgcagggt ataccaacca ggcgcagttc ttaatcaact gcggtatttt
     3781 gcaattgctg caacaggtgc cgccagagga gagtgcgcgc tatttgccga tggtggcggc
     3841 agtgcaaaaa ttgctatctc ccgcggagat gggggagttg tttaaggtgc tggcattgag
     3901 taaggggtta acgttgccgt tggttggatt tttgcaggga gataagcggc
//
LOCUS       DATMSK010000072         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_288642,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000072 DATMSK010000000
VERSION     DATMSK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288642"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..2520)
                     /locus_tag="VNZ84_02435"
     CDS             complement(64..2520)
                     /locus_tag="VNZ84_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU65837.1"
                     /translation="MIKFKHIRSRIAFTFLTVVVAIQLAGLIPLKYALVKHANTLAED
                     QIDVGQRVFVSLLQHNTQSLKQATQVLAADFAFREAVATNDAETIESALESYQGRIRA
                     QIAFYISSDENMVVGTSDAQAMLNSAQAREDAKQAITQANAGTLKFDIVDGKPYQLIT
                     VPVKAPETIGWIVMGFAVDSQLASQIKQLTHLDVTFVQKNHAQDWQLVSSTASKPLSN
                     LLLKSVADLYRQRLTLHSVELNDQTYHLKIRALHETRDEALLVVLQGSVAPFTQDLNI
                     LFKVMIFLTFVGVLIFTVLIWYVSRKTTEPIAALVKDADKMAMGDYETEIQVNSTDEV
                     GSLAKAMNDMREAVLQRSLRIQRLAFNDELTGLSNRLAFNQGVHQAIVAHANSQQKLS
                     VIVINIHRFKPINKTLGRDFGDDLLRHVGQVLKQNCLGEQDMVARLDADEFAVLLQHT
                     DQAKATQYANKVQQVFEHPVNVQGQAIDVRTGIGIALYPDHGLDEEALLNHAETALHE
                     SRSKKSPWIVYNTALEIDQSETLTLISDLKQAIVQDQLLLYIQPKIDITTRRAVGGEA
                     LIRWVHPEKGMIFPDQFIPFAEQTGVIGDITNWMLLRACQVLADFKRQGLRISLAVNL
                     STRDLNNMELPDQIAHLLSSHGLDAKALKLEITEGSLMQDPERAEVVVKKLAAMGLHL
                     AIDDFGTGYSSLAYLRQLPVQELKIDRSFVMFMDKNSGDASIVKSTIDLAHNLGLIVV
                     AEGIENEYVLNQLARLGCNEGQGYFIGKPMPEKDFSIWLERWEGQNEIDIDIGDDLSY
                     AENPLETDQQVSDPFKLDNI"
     gene            complement(2533..3123)
                     /locus_tag="VNZ84_02440"
     CDS             complement(2533..3123)
                     /locus_tag="VNZ84_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HWU65838.1"
                     /translation="MACRTRFMWWLIGLFFAIFSNQTLAGEVSVLVTDAGGKPVTDAV
                     VAFYDSKTPAVNTGATGKIVQKNKMFNPKVTIVQTGTKINFPNEDSVRHHVYSFSPAK
                     KFELKLYSGIPTDPVLFDQAGVVTLGCNIHDSMVGYIYVVDTPFFAKTDDKGKATLKL
                     NDGQYSYQAWLPGQNKPGVEQKITIEGMTEVKIPIP"
     gene            complement(3145..3567)
                     /locus_tag="VNZ84_02445"
     CDS             complement(3145..3567)
                     /locus_tag="VNZ84_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HWU65839.1"
                     /translation="MRFDVIIVVSLLCLGCMMQPKPEASLYQRIGGLPMLTRISNQTL
                     DIVSKDPKTSRSFDGVKMKTLKESLTNFLCVKTGGDCVYEGETMQKSHADLHITTAEF
                     EWMVDVLRERLDVNGVGTREKNELLKILAPMKRDVVTN"
     gene            3753..4592
                     /locus_tag="VNZ84_02450"
     CDS             3753..4592
                     /locus_tag="VNZ84_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HWU65840.1"
                     /translation="MAAFIVFIMPMLAHAGDRLLATGGVTQIEGSAGGGLMPWAVISG
                     YGTDKQIGGSLFYTHAETKGDFKLDTTGVAVGLFNRLEVSASQARFGLGDTVPGAHIR
                     LNTLGVKVRLFGDAVYEQDSWLPQVSVGAQYKYNEDFNFVPKALGAKDRSGVDYYVAA
                     SKLLLGALNGYNILLNANLQATKANQFGLLGFGGDKRDSYRIYPAVSAAVMLSDNWLV
                     GSELRYKPDNLSVYEENNAHDIFMTWFPHRNVSLTGAYIDLGRIANKDNQTGWYLSGQ
                     INY"
     gene            complement(4606..>5273)
                     /locus_tag="VNZ84_02455"
     CDS             complement(4606..>5273)
                     /locus_tag="VNZ84_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65841.1"
                     /translation="ACLDQSSLNALADKEMQYLLQRIPPAFADAVSDHLIQGQMTLKA
                     ADACQAHWQLTLPASDIAEAQALLQAEPAKQIMLAAQGYQIPEQGSVEADFAVDPATL
                     QPKHQEILQTAPLGKLRASVELMYALLTQARTSGGQAAKTPWTQAELQSAQASCQQQF
                     RADDSEKACACYSHGLAEKYSARQVKYNRYLMTNPYAFATGNGGDFKQLDKSLQAACG
                     LAI"
BASE COUNT         1277 a         1350 c         1221 g         1425 t
ORIGIN      
        1 taagggaaac ttatcatcaa gcagtaaagc ctcacttcag cgtgcgactt tataactgta
       61 ccgctagatg ttatccagct taaaaggatc ggaaacctgc tggtcagttt cgagtggatt
      121 ttcggcataa gatagatcat cgccaatatc gatatcaatc tcgttctggc cttcccagcg
      181 ctccagccag atcgaaaagt ctttttccgg catcggcttg ccaataaagt aaccctggcc
      241 ttcgttgcat cccaacctgg ccagctggtt tagaacatat tcgttttcta tgccttcggc
      301 cacgacgatg agtcctaggt tatgcgctag gtctatggtg gatttcacaa tgctggcatc
      361 accactattc ttatccatga acatgacaaa tgagcggtcg attttaagct cctgcacagg
      421 caattggcgc aaataggcca gcgatgaata accggtacca aagtcgtcaa tggccagatg
      481 caagcccata gctgccagtt ttttcacaac cacttctgca cgctctggat cctgcatcaa
      541 gctaccttcg gtgatttcaa gtttgagcgc ttttgcatcc agcccatgtg aggacaaaag
      601 atgtgcgatc tggtccggca attccatatt attcagatcg cgtgtagaca aattcaccgc
      661 cagcgagatg cgcagtccct gtcttttaaa atcagccaac acctggcagg cacgcaacaa
      721 catccagttg gtaatgtcac caatcacgcc ggtttgttcg gcaaacggga tgaactgatc
      781 cgggaaaatc atgccttttt ctggatgcac ccaacggata agcgcttcgc cacctacagc
      841 ccggcgtgta gtaatatcaa ttttaggctg gatatacagt aacagttgat cctgtacgat
      901 ggcctgcttc aaatcagaaa tcagggtcaa ggtttctgac tggtcaattt ccaacgccgt
      961 gttgtagaca atccaaggcg attttttgct tcgcgattca tgcaatgccg tttcagcatg
     1021 gttaagcaag gcctcttcat ccaaaccatg gtcaggatat aaagcgatgc cgataccggt
     1081 acgtacatca atggcctgcc cctgaacatt cacagggtgt tcaaatacct gctggacttt
     1141 gttggcatat tgcgtggcct tggcctggtc tgtgtgctgt aacagcacgg caaattcatc
     1201 ggcatcaagt cgtgccacca tatcctgctc accaaggcag ttttgtttga gcacctggcc
     1261 gacatggcgc aacaaatcgt caccaaaatc acggcccaag gttttgttaa ttggtttgaa
     1321 ccgatggata ttgatgacaa tcacactcaa cttttgctgg ctatttgcat gtgcaactat
     1381 cgcctggtgt accccctggt taaatgccag ccggttcgat aaaccggtta attcatcatt
     1441 gaacgccagg cgctggatac gcaaagaacg ctgtagcacc gcttcgcgca tatcgttcat
     1501 cgctttagca agagaaccaa cctcgtccgt actatttacc tggatctcgg tttcataatc
     1561 acccatggcc attttgtcag catccttgac caaagcagca ataggctccg tcgttttacg
     1621 tgacacatac cagatcagta cagtaaaaat tagcacgccc acaaaggtca ggaaaatcat
     1681 caccttgaac agaatgttta agtcttgcgt aaaaggtgcc accgaccctt gcaagacaac
     1741 caataacgcc tcatcgcgcg tttcatgcag cgctcttatt ttcaaatgat aggtttggtc
     1801 attcaactca acactgtgca aggtgaggcg ttgccgatag agatcggcca ccgatttaag
     1861 tagcaaattg gatagaggtt tgctggcggt gctgctcacc agttgccaat cctgtgcatg
     1921 gtttttttgc acaaaggtga catccaggtg cgtgagctgc ttgatctggc tggcaagctg
     1981 actatcgacg gcaaagccca tcacaatcca gcctatggtt tccggcgcct ttaccggtac
     2041 cgtaatcagc tggtatggct tgccatccac aatatcaaac ttgagtgtac ccgcatttgc
     2101 ttgagtaatc gcttgcttgg catcctctct cgcttgggcg gagttcagca tggcttgtgc
     2161 atctgaagtc ccaaccacca tgttctcatc actgctaatg taaaacgcga tttgtgcgcg
     2221 aatacgcccc tggtagcttt caagcgcgga ctctatggtt tctgcatcgt tggtggcaac
     2281 cgcttccctg aaagcaaaat cagccgccag cacctgggtg gcctgtttca ggctttgcgt
     2341 attgtgttgt aacaaactga cgaacacacg ctggcccaca tcaatctggt cttcagcgag
     2401 tgtattggca tgtttgacca gggcatattt aagtgggata agccctgcca attgaatagc
     2461 caccaccact gtcagaaacg tgaaagcgat gcgtgagcga atatgtttga acttgatcat
     2521 gcagtgtact gattatggga tgggaatctt gacctcggtc atcccctcta tggttatttt
     2581 ttgctctacg cctggtttgt tttggccagg caaccaagcc tggtaactgt attggccatc
     2641 atttaactta agcgtggcct tgcctttgtc atcagttttg gcaaaaaatg gcgtatccac
     2701 gacatagatg taccctacca tagaatcgtg aatgttgcat cccagcgtga ccacccctgc
     2761 ctgatcaaat aagacaggat cggtcggaat ccctgaatac agttttaact cgaatttttt
     2821 ggccggagag aaagaatata catggtgcct gaccgagtcc tcattaggga aattgatttt
     2881 cgtccccgtt tgtacaatcg tcaccttggg gttaaacatt ttattctttt gtacgatttt
     2941 accagtggcg cccgtattca ccgcaggcgt tttgctgtca taaaaagcca ctaccgcgtc
     3001 tgttacaggc tttccacccg catcggtcac cagcacactc acttcgcccg ccagcgtttg
     3061 gttgctaaag attgcaaaaa acaatccgat taaccaccac ataaaacgtg ttctacatgc
     3121 cataccaatt tccttaactg cgctttaatt tgtcaccaca tcccgcttca tgggcgccag
     3181 gatttttaat agttcgtttt tttcccgggt gccaacacca ttcacatcca ggcgctcacg
     3241 caatacatct accatccact cgaattcggc ggtcgtgata tgcaagtcag catgtgactt
     3301 ctgcatggtt tcaccttcat acacacaatc gccaccagtt ttcacgcaca gaaaattcgt
     3361 caggctttct ttcagggttt tcattttcac cccgtcaaaa gaacggctgg ttttcgggtc
     3421 cttgctgaca atatccagtg tctggttaga gattctcgtc agcatcggca aaccaccgat
     3481 acgctggtac aaactggcct ctggctttgg ttgcatcatg cagcccaggc acagcaatga
     3541 cacaacaatg atgacatcaa aacgcattac atactccata ctgtgggtcg actatcacat
     3601 attacgatat tggctattta tataaccatc aatgaaatcg gtgatttagc ctctgctcat
     3661 ccaattgttc ttatcgcgca aatgaaacaa tacgataaga aatctggagc agaatactta
     3721 tgcaatctct tacattcctt ttttcccgtg tcatggcggc attcatcgta tttatcatgc
     3781 ccatgctggc tcatgctggt gacaggcttt tagcgactgg cggcgtcaca caaattgaag
     3841 gttctgcagg cggtggcctg atgccttggg cagtcattag cgggtacggc accgataaac
     3901 aaattggtgg ctcactgttt tatacccatg ctgaaaccaa gggcgacttt aaacttgata
     3961 ccactggtgt tgctgtcgga ttgttcaatc gtttggaagt cagcgcgagc caggcacgct
     4021 ttggcctggg cgatacggtg cccggcgcgc atatccggct gaatacacta ggtgtgaaag
     4081 tgcgcctgtt cggggatgcc gtttacgagc aggacagctg gttgccacaa gtatccgttg
     4141 gcgcacaata caaatataac gaagacttca actttgtacc aaaagcattg ggtgccaaag
     4201 accgcagtgg tgtggattat tacgtggcgg ccagcaagtt attgctgggt gcactcaatg
     4261 gttataacat attgctcaat gccaatttgc aggctaccaa ggctaaccag tttggtctgt
     4321 taggatttgg tggcgacaag cgtgacagct atcgtattta cccagccgtg tctgcagccg
     4381 tgatgctgtc agacaattgg ctggtgggca gcgagttacg ctacaaaccg gacaacttgt
     4441 ccgtgtacga agaaaacaat gctcacgata ttttcatgac ctggtttcca caccgcaacg
     4501 tctctttgac gggtgcctat atcgaccttg gccggattgc caacaaggat aaccaaaccg
     4561 gctggtattt gtcagggcag attaattact aattgccttt acgcactaga tggcgagtcc
     4621 acaagccgct tgcaatgatt tgtccagttg cttgaaatct ccaccattgc cggtcgcaaa
     4681 ggcataagga ttggtcatca ggtagcgatt gtatttcacc tgtcttgccg agtatttttc
     4741 tgccagccca tggctgtagc aagcgcatgc cttttcactg tcatccgccc ggaattgttg
     4801 ctggcagctc gcttgagccg attgcagctc tgcctgcgtc cagggtgttt tggcagcttg
     4861 cccgccgctg gtcctggctt gtgtcagcag ggcatacatc agttcaacac tggcccgcag
     4921 ctttcccaat ggtgcagttt gtaaaatctc ctggtgcttg ggctgcaacg ttgccggatc
     4981 cactgcaaaa tctgcctcta cactcccttg ttcgggtatc tggtagccct gcgcagccag
     5041 catgatctgt ttggctggtt cggcctgcaa caaggcttgt gcttcggcga tatctgatgc
     5101 tggtaaggtt aattgccaat gtgcctggca agcatcggcg gcttttaaag tcatctgccc
     5161 ctgaatcaag tggtcagaca cggcatccgc aaaggccggc ggaatgcgct gcaataaata
     5221 ttgcatctcc ttgtctgcca gtgcatttaa agatgattgg tccaggcaag ctg
//
LOCUS       DATMSK010000073        12105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_217368,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000073 DATMSK010000000
VERSION     DATMSK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217368"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /gene="glyS"
                     /locus_tag="VNZ84_02460"
     CDS             complement(<1..2008)
                     /gene="glyS"
                     /locus_tag="VNZ84_02460"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWU65842.1"
                     /translation="MSTRNLLVELFVEELPPKVLKKLGESFSGVLAESLKASGLLMDG
                     SAVTTFASPRRLAAHITGVSAKAADKQVAQKLMPATVALDANGNATPALLKKLQALGE
                     DESAVAKLRKENDGKADILFLDATQAGATLQEGLQKALDETLAKLPIPKVMTYQINDG
                     WESVNFVRPAHGLVALHGHEIVPVSTLGLTAGNTTHGHRFEAKNPVVTITSADTYAQQ
                     LAEEGAVIAGFDARRAEIVKQLQAAADKQGLKPIEDEALLDEVTALVERPNVLLGQFE
                     EEFLAVPQECLILTMKANQKYFPLLDANGKLTNKFLIVSNINPADASKVIGGNERVVR
                     PRLADAKFFFDQDRKKSLESRLAGLAKVVYHNKLGSQGERNERVVAIASAIAQQIGGD
                     GFVAKVVQAARLSKVDLLTDMVGEFPELQGIMGRYYAQHEGLADDVAYAIEDHYRPRF
                     AGDDLPRSQVGVVVALADKLETLVGLFSIGEKPTGDKDPFGLRRQAIGIIRLIIESEL
                     NLPLGQLFDIASKVHNDDEIWYSAKDAELADKAQRGENTRSLRSGRILDKSSLFEFIN
                     DRLTISLKDQGYTTQEVEAVVALNNEYLFEIPRRLGAVKAFAKLAEAPALAAANKRVS
                     NILKKVEGEVKAEVKTALLQEAAEKSLYQALSDNKPKADQLFEAG"
     gene            complement(2005..2946)
                     /gene="glyQ"
                     /locus_tag="VNZ84_02465"
     CDS             complement(2005..2946)
                     /gene="glyQ"
                     /locus_tag="VNZ84_02465"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HWU65843.1"
                     /translation="MLTFQQIILKLQEYWDKQGCTLLQPYDMEVGAGTSHTATFLRAL
                     GPEPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPANILELYLGSLEALGF
                     NLKENDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGINCKPITGEITY
                     GLERLAMYLQGVENVYDLEYSPGVKYGDVFHQNEVEQSTYNFEHSDADFLFTAFNAHE
                     KQAKHLMEVKLALPAYEQVLKAAHTFNLLDARGAISVTERAAYIGRIRNLARAVAASY
                     LDSRARLGFPMAPKAWADEVIAKIESEQAEQNKKAAA"
     gene            3100..3537
                     /locus_tag="VNZ84_02470"
     CDS             3100..3537
                     /locus_tag="VNZ84_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65844.1"
                     /translation="MKYYRTWVLLLTLWFALLQTIAPLMHAHAAGDTAADDGPHMHMV
                     DIPALHADTAETTKHPFHWHVHSNKSHGQIIGLGQAISENNTWLSLIDAYANLWLTAL
                     LPSFFLLSLCLLPLFSQFERQKNDSSHQRPPLTRHSPRGPPLA"
     gene            3661..4881
                     /locus_tag="VNZ84_02475"
     CDS             3661..4881
                     /locus_tag="VNZ84_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWU65845.1"
                     /translation="MLGHAVVALAESPGPIDSDHIDTVSTNPHLTLHDVLQQVVAIHP
                     QQSLLAAHQQMVQARKVMANSWLPQAPSVGFSHQSDAFLSHRDEREWQAQVQIPIWLP
                     GQRQARDEVATLADNSLLQDRAGLQQLAADLLRNAVWDIALRRNDINLMENRRNTLRS
                     IGEDVQKRFKAGEIARMDVMQSQQETIQAERQLVLATAELMHAQFRYQQLTGLKEMPA
                     RLEEPLSTRETYTDSAFWQAAIARVRLAEGQRNLTMIEHRQNPQLTINTRTMQGGFDS
                     QFNGSMGVAINIPLQSEVTRAPQLANAEQNIGDAKSQLETLQRTLETALHEAEHNLHV
                     SRQELSLIEQQQAIASENATLARKAYRLGELDLNQLLRLQLLAFEAERSLSSQQLQVQ
                     WNIAKYNQAVGVLP"
     gene            4878..5966
                     /locus_tag="VNZ84_02480"
     CDS             4878..5966
                     /locus_tag="VNZ84_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU65846.1"
                     /translation="MKRMLCLTLSTCLSLNTLAATLLPLSPAQLKQMQVQWGHLRAEQ
                     SGLSASYPAEVVLPSTQTRVVTAPQGGLITRLSVSMGDTVKQGQVIGQLSSPDLVVLQ
                     SGFLQSDTQARLHEQALKRDQELFKDGIISQRRMQETQSAYETSQALMHERKQTLKLA
                     GMSDAQVQQLSQKRNMQNGVAIIAPISGQIMEQQQQVGARVDMSMPIYTISNPKPLWL
                     MIQMPIAQAQQLSPGAMVRLPKQNLTAKVDTVLRQLNKNNQTTQVRASISEGAEQLSV
                     GQVVDVALVEHTAQGSFSLPRTAVARQGEQAVIFVQQAKGVAVTPVKVVLEQDQQLTV
                     QGSLNGNEMIAVQGVAALKGHWLGLGGE"
     gene            5970..9062
                     /locus_tag="VNZ84_02485"
     CDS             5970..9062
                     /locus_tag="VNZ84_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWU65847.1"
                     /translation="MLDWLIKTSLKQRLLICVFAIGLLFAGWTAYQTIPIDAFPDVSS
                     TQVKIIIKSPGMTPEEVEARITAPIEVEMLGIPKQTSLRAVAKYALTDITIDFEDGTD
                     IYWARQQVTERLNNAWPNLPTEISGGIAPMTTPLGEMFMFTIESDTLSLQEKRTLLDW
                     VIRPRLRTVPGVADVNALGGLVRSFEIVPDNAKLQARGLALSTLQQAISENNRNDGAG
                     RLKDGEESLLVRAEGAFKTLDDVRNTVLKGQQGNPVRIADVAEVRIGELTRYGAVTRN
                     GQGETVEGLVLGLRGANARQVVDGVRAKLDEIAPTLPKGVNIHVFYDRGNLVEHAVHT
                     VNKALMEAVVLVLILLVLFLGNLRAALTVACALPLAMLATFWLMQQYGMSANLMSLGG
                     LAIAIGMLVDSAVVVVENIVEHLAHSQASQAQDKLSVILAAMREVSVPVTAGIVIIIT
                     VFLPLLSLQGLEGKLFAPVALSIMFALGSSLILSLTIIPVLASFLIRDHATEDPWLIR
                     HLNRWYSRTLSTAMQHKRAVLLAAMSLLVLTAIVYPLIGKTFMPTMDEGDIIIGTEKL
                     PSIALPQSIATDGHLQKTLLQQVPELQGVYSRAGSDELGLDPMGVNQTDNFLALKPRD
                     QWQVPDKATLIEKLRQVMHGIPGLDYNFTQPIEMRVNEMILGVRGDLALKIFGSDLKT
                     LDQKAREIIKVLESIPGNQDVYTPQNSGVQYLQIKIDRVAAGRLGLNVADINALLQSQ
                     LEGKLLGIVLEGNRRTPILLRGNQIVRESASDFSQLMLALPQGGTVPLSSVANIVREE
                     GPVKIDRERGNRMVVVTANVKGRDLVSFVEEAKQKVAQQVKLPEGYWLKWGGQFENQQ
                     RAAARLSIVIPIAIALIALLLFMTFGSLPKALLILANIPFAMIGGVVALWLSGEYLSV
                     PASVGFIALLGIAVLNGVVMVSHFQHLAEQGMAAEKIAYEGARRRLRPVLMTASITAF
                     GLVPLLFATGPGAEIQRPLAIVVIGGLITATFLTLFVLPILYQHFFGNETLESNVSEF
                     "
     gene            complement(9134..10360)
                     /gene="argJ"
                     /locus_tag="VNZ84_02490"
     CDS             complement(9134..10360)
                     /gene="argJ"
                     /locus_tag="VNZ84_02490"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWU65848.1"
                     /translation="MPVKLSAPAAETLLPVKGVQLGFAQAHIRKPNRKDVLVMTVPEG
                     SSVSGVFTLNRFCAAPVTVCKEHLKLGKGIRALLVNTGCANAGTGQQGLDDARASCIG
                     VAQALNISPEQVLPFSTGVILETLPMERLLAGIAPAVANLQPDNWLNAAEAIMTTDIV
                     AKGTSRQLTIDGKTITITGISKGSGMIHPNMATMLGYIATDAAISQTALDSIIQHAVN
                     KSFNCITVDGDTSTNDSLILMATGQAGNALINEQSADFAILRDTLTEVAIELAQAIVR
                     DGEGATKFMTIQVEAGKNEEECRKVAYAIAHSPLIKTAFFASDPNLGRILAAIGYAGI
                     NDLDVNTMQLYLGDVLVAEKGGRAASYVEEQGAAVMKESDILVRVVLNRGDANATVWT
                     CDFSYDYVKINADYRS"
     gene            complement(10404..11762)
                     /gene="cysS"
                     /locus_tag="VNZ84_02495"
     CDS             complement(10404..11762)
                     /gene="cysS"
                     /locus_tag="VNZ84_02495"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWU65849.1"
                     /translation="MLKLYNTLSRSKQDFKPITDKKVNMYVCGMTVYDFCHLGHARVM
                     VVFDMVARWFRASGYEVTYVRNITDIDDKIIKRANENNESIQSLTQRFITAMDEDSAK
                     LGIIRPDIEPRATEHVQGMLDMISQLINKGFAYQADNGDVFYKVRSFNDYGKLSGKSL
                     DDLRAGERVDVDGHKQDPLDFVLWKSAKPGEPYWESPWGNGRPGWHIECSVMSAEHLG
                     QHFDIHGGGQDLQFPHHENEIAQSEATHGCTMANYWMHNGFIRVDDEKMSKSLGNFFT
                     IREVLEKYDPEVVRFFILRAHYRSPLNYSDKHLDDAKASLTRLYTALRGIDVADSAID
                     WALPQAVKFKAAMDDDFNTPEAIAVLFELANDLNKDKSAATAALLKQLAAIIGLLQRD
                     PESFMQGESDSALDVERLIAARIAAKTAKNYAEADRIRKALADAGIILEDTPQGTTWR
                     RA"
BASE COUNT         2929 a         3250 c         3028 g         2898 t
ORIGIN      
        1 caccagcttc aaacaattga tcagctttcg gcttgttatc ggacagcgcc tgatataaag
       61 atttctcagc agcctcttgc agcaacgctg tcttcacttc tgccttcact tcgccttcga
      121 cctttttcaa aatgttggac acacgcttgt tagctgcggc caatgcgggt gcttctgcta
      181 gtttggcaaa agcttttacg gcacctaagc ggcgaggaat ttcaaataaa tattcgttat
      241 ttaaagcaac gactgcctca acttcttgtg ttgtgtaacc ttgatctttt aagctaatag
      301 ttaatctgtc attaataaat tcgaaaagag aagacttatc gagaatccta ccggacctca
      361 atgatctagt attttcacct ctttgggctt tatcagctaa ctccgcgtcc ttagcgctat
      421 accaaatttc atcatcgtta tgcactttgg atgcaatatc aaataattga cctaaaggta
      481 agtttagttc tgactcaata attaatctta tgattccaat tgcttgcctt ctcaaaccaa
      541 aaggatcttt gtcgcccgtg ggtttttcac ctatgctaaa taatccaacc agcgtctcca
      601 atttatctgc caaggccacc accacaccga cctggctgcg tggtaaatca tcaccggcaa
      661 agcgtgggcg gtaatggtct tcaatagcat aggccacatc atcggccaag ccttcatgct
      721 gggcatagta acggcccata atgccttgca gctccgggaa ctcaccgacc atgtcagtca
      781 gcaggtcgac tttggataag cgtgcagctt gcaccacttt agccacgaag ccatctccac
      841 cgatttgctg agcaatggca gaagcaatcg ccaccacacg ctcattacgc tcaccttgtg
      901 aacccagctt gttgtggtaa accactttag ccaagccagc caaacggctt tccagcgact
      961 ttttacggtc ctggtcaaag aagaatttgg catccgccaa gcgtgggcgc accacacgct
     1021 cgttaccgcc aatgaccttg ctggcatcag ccggattgat gttagagaca atcaaaaatt
     1081 tattggtcaa cttaccgttc gcatccaaca gcgggaagta tttctggttg gccttcatgg
     1141 tcaggatcag gcactcttgc ggcacagcca ggaactcttc ttcaaactgg cccagcaaga
     1201 cattcgggcg ttccaccagc gcggtcactt catccagcaa cgcctcatct tcaatcggct
     1261 tcaggccttg cttgtcggcg gcagcttgca gttgtttcac aatctcggca cgacgcgcat
     1321 caaacccggc aatcacggca ccctcttccg ccagctgctg cgcataggtg tctgcagaag
     1381 taatcgtgac cactggattt tttgcttcaa agcggtggcc atgcgtcgta tttccagccg
     1441 ttaaacccag cgttgaaaca ggcacaattt catgaccatg caaggccacc agaccatggg
     1501 ccggacgcac aaagttgacg ctttcccagc catcattgat ctggtaagtc atcactttag
     1561 gaataggcag ttttgccaag gtttcgtcca aagctttctg caagccttct tgtagcgtgg
     1621 cacctgcttg cgtggcatcc agaaacagga tatcggcttt accatcattc tctttgcgca
     1681 gcttggccac cgcggactca tcttcgccta aagcctgcag ttttttcagc aaggctggtg
     1741 tcgcattgcc gttggcatcc agcgctactg tggccggcat cagcttttgc gcaacttgtt
     1801 tatcggcggc ttttgctgac actccggtaa tatgcgcagc caggcgacgc ggggaagcaa
     1861 aggtagtcac cgcgctgcca tccatcaaca ggcccgaagc cttcaggctc tcagccagta
     1921 cgccactgaa agattcaccc agttttttta ataccttggg tggcagttct tctacaaata
     1981 attcaactaa caggttgcgg gtactcatgc ggcggctttc ttgttttgtt ctgcttgttc
     2041 gctctcaatc ttggcgatga cttcatctgc ccaggctttt ggtgccatcg ggaagcccag
     2101 tcgggcgcgg ctatccaggt agctagccgc gacagcacgg gccaagttgc ggatacggcc
     2161 tatgtaagcc gcacgttccg tcaccgaaat cgcgccacgc gcatccagca ggttaaaggt
     2221 atgcgcggct ttcagcactt gttcatacgc aggtaaagcg agcttcactt ccatcaggtg
     2281 cttggcctgt ttttcgtgcg cattaaaggc agtgaacagg aaatctgcat cagaatgctc
     2341 aaagttatag gtcgattgtt ccacctcatt ttggtggaac acatcgccgt atttcacgcc
     2401 tggtgaatac tccaggtcat agacgttttc cacgccttgc aggtacatgg ccagacgctc
     2461 cagaccgtag gtaatttcac cggtaatcgg tttgcagtta atgccgccca cttgctggaa
     2521 gtaagtgaat tgtgtgactt ccatgccatt cagccagact tcccagccca ggccccaggc
     2581 acccaaagtc gggttttccc agtcatcctc aacaaaacgg atatcgtttt ctttcaggtt
     2641 aaagccgagg gcttccaacg aacccaaata gagctccaga atattggccg gtgcaggttt
     2701 cagcaccacc tggtattggt aataatgttg taaccggttt gggttttcac cataacggcc
     2761 gtctttgggg cgacggctgg gctgcacata agcggctttc cagggctcag ggcccaatgc
     2821 acgtaaaaag gttgcggtgt ggctggtccc tgcacccact tccatatcgt aaggttgcaa
     2881 caaagtgcag ccttgtttgt cccagtattc ctgaagcttt aaaataattt gttgaaaggt
     2941 cagcaccatc gcgcctgatc cgattgattg taaaacgaat attttacctg attcagtcag
     3001 ggttaagcaa tcgcattaca gtaccgttag aagagttaga tggttttttt acggtttttt
     3061 gcgattgtga cttcacggaa actctgatag attaggcgca tgaaatacta ccgcacatgg
     3121 gtattgttgc tgacgctttg gtttgccttg ctgcaaacca tcgcgccgct gatgcacgcc
     3181 catgccgcag gcgatactgc cgctgacgat ggcccgcaca tgcacatggt cgatattcca
     3241 gcgttgcatg ccgatacagc cgagacgacc aagcatccgt tccactggca tgtgcattcc
     3301 aataaatcac atggccaaat catcggtcta ggccaagcaa tcagtgaaaa caatacctgg
     3361 ttatctttaa tcgacgccta tgccaatctg tggctaaccg ctttactgcc cagttttttc
     3421 ctactgtcat tatgcctgtt accccttttc agtcagtttg aacggcagaa aaacgacagc
     3481 tcacaccaac gcccacccct cacgcgccac agcccacgtg gcccaccact cgcctgaaca
     3541 ttgactcgtt gcgtcggttg gcattagcca accgcttgcc tgttttgttc aggagtatgt
     3601 atgtttcaat taagcaaggg agcgtgtcgc tccctgttaa tcacatggtg catatggctg
     3661 atgcttggcc atgcagttgt tgccttggcc gaaagcccgg ggcccattga cagcgaccat
     3721 atcgacacgg tttccactaa cccgcatttg accttgcacg acgtattgca gcaagtggtt
     3781 gccattcatc cgcaacaatc tttgctggcg gcgcaccaac agatggtgca ggcccgcaaa
     3841 gtcatggcca acagctggtt gcctcaagct ccctcggtgg gtttttccca ccagagtgat
     3901 gcgttcctta gtcatcgtga cgagcgcgag tggcaagcac aagtacaaat cccgatctgg
     3961 ttgcccggac agcgccaggc acgtgacgag gtagccacac tggcggataa cagcctgtta
     4021 caagaccgtg ccggcttgca gcaactggcg gcagatctgc tacgcaatgc agtatgggac
     4081 atagccttgc gccgtaatga catcaacctg atggagaatc gtcgcaacac gctgcgcagc
     4141 attggcgagg atgtacaaaa acgtttcaag gcgggtgaaa ttgccaggat ggacgtgatg
     4201 caatcgcagc aggaaaccat acaggccgaa cggcaactgg tcctggcaac cgcagagtta
     4261 atgcatgcgc agtttcgcta ccagcaattg accggactaa aggaaatgcc ggccaggtta
     4321 gaggaaccgt tgtccacacg ggaaacctat accgactctg cattttggca agccgcaatt
     4381 gcacgtgtca ggcttgctga aggtcaacgt aacctgacca tgattgagca tcgccagaat
     4441 cctcaattga cgatcaacac ccgtaccatg caaggcgggt ttgacagcca gtttaacggt
     4501 agcatgggtg tcgcaatcaa catcccactg caaagcgagg tcacgcgcgc gccacagctc
     4561 gccaatgccg aacaaaatat cggggatgcc aaaagccaac ttgaaacatt gcagcgcaca
     4621 cttgaaacgg cactacacga agccgagcac aacctccatg tcagccgcca ggaactctca
     4681 ctgattgagc agcaacaggc tatcgccagt gaaaatgcca cgctcgcccg caaagcctac
     4741 cgtttaggtg aattagacct caatcaatta ttacgcctgc aactgctggc ctttgaagca
     4801 gagcgcagct taagtagcca gcagttacaa gtgcaatgga acattgccaa atataaccag
     4861 gccgtaggag tcctcccatg aaaagaatgc tatgtttaac cctctccacc tgcctgtccc
     4921 taaacaccct ggcagcaaca cttttaccct tgtcaccggc acaactgaaa cagatgcaag
     4981 tgcaatgggg ccatttgcgg gcagagcaaa gcggactatc cgccagctac ccggctgaag
     5041 tggtattgcc gtcaacacaa acgcgtgtgg tgacagcgcc gcaaggcgga ttgattacac
     5101 gtttaagcgt aagtatgggc gatactgtca aacagggcca agtcatcggc cagctcagca
     5161 gcccggacct ggtggtcttg caaagtggct ttctacaaag cgacacccag gcccgcttgc
     5221 acgaacaagc gctgaaacgc gaccaggagc tattcaaaga cggcattatt tcccaacgcc
     5281 gcatgcagga aacacaaagt gcttacgaaa ccagccaggc gctcatgcat gaacgcaaac
     5341 aaaccctgaa actggcgggc atgagtgatg ctcaagtcca gcagctttcg caaaaacgca
     5401 atatgcaaaa cggtgtggcg atcatcgccc ccatcagcgg acaaatcatg gaacagcaac
     5461 agcaagtcgg cgcacgtgtc gatatgtcca tgcctatcta caccatcagc aatcccaaac
     5521 ccctgtggct gatgatacag atgccgattg cgcaagcgca gcaactgagc ccgggcgcaa
     5581 tggttcgttt acccaagcag aacctgacgg ccaaggtgga tactgtgtta cggcagttga
     5641 ataaaaacaa ccaaaccacc caggtcaggg cttcgattag cgaaggtgca gaacagctgt
     5701 cagttgggca agtggtcgat gtggcactgg ttgagcacac ggcgcaaggt agttttagcc
     5761 tacccagaac agccgtcgcc cgacaaggag agcaagcagt aatctttgtg cagcaagcaa
     5821 aaggcgtcgc cgtcaccccg gtaaaagtag tgcttgaaca agatcagcag ttaaccgtgc
     5881 aagggtcact gaatggtaac gagatgattg ccgttcaggg cgtcgctgca ttgaaaggcc
     5941 attggcttgg cctgggtggg gagtagacca tgttagattg gctgattaaa acttcgctca
     6001 agcaacgact gctgatctgc gtgtttgcca tcggtctgct atttgcaggc tggacggctt
     6061 atcaaaccat cccgattgat gccttccccg atgtatcgtc tacccaggtc aagatcatca
     6121 tcaagtcgcc ggggatgaca ccggaagaag tagaagcaag aattaccgca ccgattgaag
     6181 tcgagatgct gggcatcccc aaacaaacga gtttacgcgc agtggctaaa tacgcgctga
     6241 ccgatatcac catagacttt gaggatggga cggatattta ctgggcgcgc cagcaagtca
     6301 ccgagcggct gaacaatgcc tggcccaact tgcctaccga gatttccggc ggcattgccc
     6361 cgatgacaac gccgctgggc gaaatgttta tgtttaccat tgagtccgac acactcagcc
     6421 tgcaggaaaa acgcaccttg ctagactggg tgatccggcc gcgcttgcgc acggtgccgg
     6481 gcgtggcgga tgtcaatgcc ttgggcggcc tggtacgcag ctttgaaatc gttcccgata
     6541 acgccaagct gcaagcacgc ggcctggcct taagcactct gcaacaagcc atcagcgaaa
     6601 ataaccgtaa cgatggtgcc ggccgcctta aggatggcga agagtcttta ttagtacgcg
     6661 cagagggcgc tttcaaaacc ctggacgatg tgcgcaatac cgtactcaaa ggccaacaag
     6721 gcaatccggt gcgtatcgca gatgtggcag aagtacgcat tggcgaactc acgcgttatg
     6781 gcgcggtgac ccgaaatggc cagggtgaaa cggtagaagg cctggtgctg ggattgcgcg
     6841 gtgccaatgc acgccaggtg gtggatggcg tacgtgccaa gctggacgaa atcgcgccaa
     6901 ctttacccaa aggcgtcaat attcatgtgt tttatgaccg tggcaaccta gtcgaacatg
     6961 ctgtgcacac tgtgaataag gccctgatgg aagcggtggt actggtcttg attttactgg
     7021 tgctattttt aggtaacctg cgtgcagcac tcactgtggc ctgcgccttg ccgctggcca
     7081 tgctcgcgac cttctggctc atgcagcaat acggcatgtc cgccaacctg atgtcactcg
     7141 gtgggctcgc gatcgcgatc ggtatgttgg tcgattccgc agtggtggtg gtagaaaata
     7201 ttgtcgagca tcttgcacac tcgcaagcct cacaagcgca agacaaattg agcgtcatac
     7261 tcgccgccat gcgcgaagtc agcgtaccag tgacagcggg cattgtgatt atcatcaccg
     7321 tatttttgcc cttactttcc ttgcaaggtc tggaaggcaa actgtttgcg cctgtcgccc
     7381 tcagcatcat gttcgccctg ggttcctcat taattttatc gctcaccatc attccggtgc
     7441 tggcctcgtt cctgatccgc gaccatgcga ctgaagaccc ttggctgatt cgccacctta
     7501 atcgctggta ctcgcgcacg ctcagcaccg cgatgcaaca caaacgggcc gtgctgctgg
     7561 ccgcgatgag cctgctggtg ctcaccgcga ttgtttatcc actcataggc aaaacattta
     7621 tgccaaccat ggatgaaggc gacattatta tcggcaccga gaagctgcca tcgatcgcct
     7681 tgccacaaag catcgctacc gatggtcacc tacaaaaaac attgctgcaa caggtgccag
     7741 agctgcaagg cgtatattcc agggcaggtt cggacgaact tggcctggac ccgatgggcg
     7801 ttaaccagac ggataatttt ctagcactca agcctcgtga tcaatggcag gtaccggaca
     7861 aagccacgct gatcgaaaag ctacgtcaag tcatgcacgg cattccaggc ctcgattaca
     7921 acttcaccca acccatcgag atgcgcgtga atgagatgat tcttggtgtg cgcggtgacc
     7981 tggcgctcaa gatttttggc agcgacctca agacactgga tcaaaaagcg cgggaaatca
     8041 ttaaagtgct ggaatccatc cctggcaacc aggatgtcta taccccgcaa aatagtggtg
     8101 tgcaatattt gcaaatcaag atagatcgtg tcgcggcggg caggttaggc cttaacgtag
     8161 ccgacatcaa tgccctgctg caaagccagc tggaaggtaa gctgctaggg attgtgctcg
     8221 aaggcaaccg ccgcacccct atcctgctgc gcggcaacca gattgtacgt gagtctgcct
     8281 cagacttcag tcaactcatg ctggcactgc cgcaaggcgg caccgtccct ctctccagcg
     8341 tagccaacat cgtccgcgaa gaaggtccgg ttaagataga ccgcgagcgc ggcaaccgca
     8401 tggtcgtcgt caccgccaat gtcaaaggcc gcgacctggt cagctttgtt gaagaagcca
     8461 aacaaaaagt ggcacaacaa gtgaagttgc ccgaaggcta ctggctaaaa tggggaggac
     8521 aattcgaaaa ccagcaacgg gccgcagcac gcctgagcat cgtcatcccg atagcgattg
     8581 cactgattgc cctgctgcta ttcatgacct ttggctccct ccccaaagca cttctgatac
     8641 tggccaacat cccattcgcc atgattggtg gcgtcgtcgc attgtggctg tcaggtgaat
     8701 acctgtcagt cccggcttca gtgggcttta ttgccctgtt agggattgca gtgctgaatg
     8761 gcgtcgtcat ggttagccat tttcagcatt tggccgagca agggatggct gccgaaaaaa
     8821 ttgcttatga aggcgccaga cgacggctga gaccggtctt gatgacagcc agcattacgg
     8881 cgtttggcct ggttccgctg ttgtttgcaa ccggtccggg ggcggagata caacgcccat
     8941 tagcaattgt ggtgattggt gggttaataa ccgcgacgtt cttaacgctg tttgtgttgc
     9001 cgattttata tcagcatttt ttcggtaatg aaacattaga aagcaatgtt tctgagtttt
     9061 gagatagtgc gatgattaag gcgttcttgt tgccactagg caataagaac gccgcttaaa
     9121 tgggtacaga atattacgag cgatagtccg cgttaatctt cacatagtca taagagaaat
     9181 cacacgtcca taccgtcgca tttgcgtcac cacggtttaa caccacgcgc accaagatat
     9241 cgctttcctt catcacggca gcaccttgtt cttcgacata gctcgctgcg cgaccgcctt
     9301 tttctgcaac caatacatcg cccagataca attgcatggt attcacatcc agatcattaa
     9361 taccggcata gccaatggcg gcaaggatac ggccgagatt agggtctgag gcaaagaatg
     9421 cggttttaat cagcggcgag tgggcaatgg cgtaagccac tttgcggcac tcttcttcgt
     9481 ttttgcctgc ttccacctga atagtcatga atttagtcgc gccctcaccg tcgcgcacaa
     9541 tcgcttgcgc cagttcaatc gccacttctg tcaacgtgtc gcgcaagatt gcaaagtccg
     9601 cactctgttc attgataagt gcattacctg cctggccagt ggccatcagg atcaagctgt
     9661 cgttggtact ggtgtcgccg tcaacggtaa tacaattgaa ggatttattt acagcgtgct
     9721 ggatgatgct atcaagcgcc gtctggctga tcgccgcatc tgtggcaata tagcccagca
     9781 tggtcgccat atttggatga atcatgcccg agcctttgct gatgccggta atcgtgattg
     9841 ttttgccatc aatggtcagt tgacgtgaag tgcctttggc cacgatatcc gtagtcataa
     9901 tggcttccgc tgcgtttaac cagttgtctg gctgcaagtt ggcaaccgct ggggcaatgc
     9961 ccgccagcag cctttccatc ggcagcgttt caaggatcac gcctgtagaa aaaggtaaca
    10021 cttgctcggg gctgatgttc agtgcttgtg ccacgcctat gcagctggca cgcgcatcgt
    10081 ccaagccttg ctgaccagtg cctgcattgg cgcagccagt gttaaccaac agggcacgga
    10141 tacctttacc gagcttcaaa tgctctttac acaccgtcac cggtgccgca caaaaccggt
    10201 tcaaggtgaa tacgccagaa acactgctgc cttcaggcac agtcatcacc aacacgtcct
    10261 tgcggtttgg tttgcggata tgtgcctggg caaaacccag ttgtacgcct ttgacgggca
    10321 gtagtgtttc ggcagcaggg gcagacaatt tgacgggcat ccgtgttcct tcaatgtttc
    10381 aaaagaataa tgttgttacg caatcaggcg cgacgccaag tcgtaccttg cggagtatct
    10441 tccagaataa tgccggcatc cgccaacgct ttgcggatgc ggtcagcttc ggcgtagttt
    10501 ttggctgttt tagccgcaat acgcgctgca atcaggcgct cgacgtcgag cgcactgtcg
    10561 ctttcacctt gcataaaact ctccgggtcg cgttgcaaca ggccgataat cgcagccaat
    10621 tgtttcaaca atgcggcagt ggcggcggat ttgtctttgt ttaggtcgtt ggcgagttca
    10681 aacaagacgg cgatggcttc tggtgtattg aagtcgtcgt ccatggcagc cttgaatttg
    10741 acggcttgcg gcaatgccca gtcgatggcg ctatcagcaa cgtctatgcc acgcaatgcg
    10801 gtatacagcc gcgtcaatga tgcttttgca tcatccaggt gcttgtccga ataattgagc
    10861 gggctgcggt agtgcgcgcg caggataaag aatcgcacca cttccgggtc gtatttttcc
    10921 aatacttcac gaatggtaaa gaaattgccc agtgacttgg acattttctc atcatcgacg
    10981 cggatgaagc cgttatgcat ccagtagttg gccatggtgc aaccatgggt ggcctcggat
    11041 tgtgcaatct cgttttcatg atgcgggaac tggaggtcct gcccgccacc gtgaatgtca
    11101 aagtgctgtc ccagatgttc ggcactcatc accgagcatt caatatgcca gccagggcga
    11161 ccattgcccc atggcgattc ccaataaggc tcaccaggtt tggcggactt ccatagcacg
    11221 aagtcgagcg ggtcctgctt atggccgtcg acatccacac gttcgccggc acgcaaatca
    11281 tccaggcttt tgcctgaaag cttgccgtag tcgttaaagc tgcggacctt gtaaaacacg
    11341 tcaccattgt ctgcctgata agcaaaacct ttattgatga gctgactgat catgtccagc
    11401 atgccctgta catgctcggt agcacgtggc tcaatgtcgg gacgaataat accgagtttg
    11461 gctgaatctt cgtccatggc agtaatgaaa cgctgcgtca ggctttggat actttcgttg
    11521 ttctcgttgg cacgtttgat gattttgtcg tcaatgtcgg taatattacg cacataagtc
    11581 acctcgtaac cactggcacg aaaccagcgc gccaccatat caaacaccac catcacgcgc
    11641 gcatggccaa gatggcaaaa gtcatacacc gtcatgccgc agacatacat attgaccttt
    11701 ttgtctgtga tgggcttaaa gtcttgcttg ctacgggaaa gggtgttgta tagttttaac
    11761 atgctggaga aatatggatg gatgaatacg tgacaaaatt agcggaagtg tcgtagtatt
    11821 taatttaggt taagctcgag gtgcttgtat attcttagta tcggcgagag gtaccactgc
    11881 ttgatgattt gttgcctggt aaccacgcgc gacacaaatt tgatgcttaa tcttcgcgta
    11941 ataatcacct aaacgcgccc tgtctcttgt acaatccaag tataacatac tgctaaaaat
    12001 caacgattgc tcaggtaata aattcatgaa tgcaacatta aaaaaatatt tactggtttt
    12061 gggtgtcttg ttaagccaaa ccgccttctc ttatcatgct ttcgc
//
LOCUS       DATMSK010000074        23664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_147194,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000074 DATMSK010000000
VERSION     DATMSK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 23664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23664
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147194"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VNZ84_02500"
     CDS             <1..186
                     /locus_tag="VNZ84_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HWU65850.1"
                     /translation="HFQRAIDEIDKSIDHLQKTKDALLSTSRNLRLANDKAQDVTIKK
                     LTKGNPTMAEKFEGLKR"
     gene            complement(239..577)
                     /locus_tag="VNZ84_02505"
     CDS             complement(239..577)
                     /locus_tag="VNZ84_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HWU65851.1"
                     /translation="MNPIEWNDFTKVELRVGRVISAEVFAEARKPAYKLHIDFGPEIG
                     IRKSSAQITAHYTPENLIGKLVIAVVNFPPKQIGPLMSECLVTGFHDAEGRVTLCVPE
                     HEVPLGTRLL"
     gene            complement(630..1184)
                     /locus_tag="VNZ84_02510"
     CDS             complement(630..1184)
                     /locus_tag="VNZ84_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWU65852.1"
                     /translation="MNQVIASTDAVWMLIDVQTRLCSVMPEATMATVLKNIQRLLQGA
                     QLLNIPLWVTEQYPEKLGETLPELQALLPEYTPRTAKLAFSAWHEPALQESSISVAPQ
                     VVLFGLETHICVLQTALDALAAGKQVFIVEDAVISRTEANRTNAIARMRSAGCIITNT
                     ESMLFEPMQGATHAQFKAVSALIR"
     gene            complement(1233..2579)
                     /locus_tag="VNZ84_02515"
     CDS             complement(1233..2579)
                     /locus_tag="VNZ84_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HWU65853.1"
                     /translation="MSLNHLPRLIVLASSLWLPVTTWAASQSLVDIYQEAKANDPVLA
                     SAGSANLAAQEIIEQAKAGYRPSVNFTAGANANRTDLKIVGSQAPFPGGRNTYEGYQA
                     QLQARQPIFRKENLERIDQSKVQVSIADKQYHLAQQNLMLRTTQAYFDVLLAQDRMTL
                     IEAQKKAIQSQLQQAKANFDVGTATITDFNEAQARYDLIAAQELSAFNEIEIAKRSLQ
                     AITGKLPEQLASVRADIQITPSLKPMQEWQEVTNASNLNIQIQQEMVQVNEKDIEIAR
                     AGHYPTVDAVANYQDSYTNGGQYGFGGDLKSASIGVEVSVPLYQGGAVTSRVRQAAYN
                     KQKALDDLQNALRQTSLETQRAYLNLSTSIAQVKALEQALKSSQSQLDSTQLGYEVGV
                     RTSVDVLNAQQQYYSANRDLLQARYNYLVNIIRLKTASGIVSEADLQDIEQQLAKR"
     gene            complement(2592..3248)
                     /locus_tag="VNZ84_02520"
     CDS             complement(2592..3248)
                     /locus_tag="VNZ84_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HWU65854.1"
                     /translation="MTDQATARFNMIEQQIRTWEVLDPQVLATLNNMPREAFVPEAYR
                     GLAFADIEIPLAHGQQMLSPKIEGRFLQALQLQAKDKVLLIGAGSGYLAALMAQHCAQ
                     VTALEVFPELCALASQNLTKQSIQNVQVIEIDGHNGYESQAPYDAIIFSASSPVEPEG
                     VRYQLSIGGRMLIVLGQAPVMQATLIQRLAEHSYKQDVLFETCLTEMQNAPQAQAFSF
                     "
     gene            complement(3283..6435)
                     /locus_tag="VNZ84_02525"
     CDS             complement(3283..6435)
                     /locus_tag="VNZ84_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU65855.1"
                     /translation="MSAPEHTSDAHIDGQPAHHRFNLSTWALKNQALVLYFILLTLLA
                     GAFAYTHLGQSEDPPFTFKVMLVRSSWPGASAQEVEQQITDKIEKKLQEIPSIDYTSS
                     YSRPGESVVFIVIRDNTFSEKIPELWYQVRKKIGDIRHTFPSDLQSLTFNDEFSDVYG
                     TMYAITADGLNPQQLKRQAEWVRARLLKLPDVEKVDLFGEQPQKIIIEISNQKLTSLG
                     ITPAQLAQLLQQQNKVIASGTFDAGPERIRLDVSGRLQTLEDLRQFPIRAGSQNLTLG
                     EIATIKRGYAEPPAERIHFNGQSALLIGVSMQQGGDVIALGKAMQQIITSVQKQLPLG
                     VNLQLVTSQPDIVQHSVHDFVRSLTEALIIVLAVSLLSLGWRTGIVVAVAIPIVLAGT
                     FLLMDWLDIGLHKISLGALILALGLLVDDAIIAVEMMAAKMEQGWERMQAASFAFTST
                     AMPMLTGTLVTVAGFLPIATAVSSTGEYTRSIFQVSAIALTLSWVAAVIVIPFLGYHL
                     LPDAKHPHNEASAHNQASIYETSFYRRLQSTVAWCVNYKGSVIIATIILFVVAMLSFG
                     KVQQQFFPDSTRLEIIVDLRLAENASDQSTETAVKQLEQWLQGWQQAHGKLENVVSYL
                     GAGAPRYYLPLDVQLPHRAFGQLVILTNTLEQREALRNDLLTLFEHDFPQLRASVLRL
                     ENGPPVGFPVQFRVDGQNLQAIREVAHKIADAMRANPHLRNVQLGWEEPSKVIHVDID
                     QHKARMLGVNSPDIAQLLNTQYFEQVVSEFREGNERIDLVMRSTQQEHTQLSQLAQLT
                     VLSQRGERVPLAQVANIHYGFEEGVIWRRNRIPSLTVRAHLRGSMQAPVVSAQIQQVI
                     QPILQHLPAGVTVETGGAIEESAKGATSVAKGVPLFLAAVFTLLMLQLRSISNVILVV
                     LTAPLGMIGITFALLMFNMPFGFVAMLGSIALSGMIMRNSVILVDQIKQDIAEGASLE
                     QAVVQSTVRRFRPIVLTAAAAILAMIPLTRSVFFGPMAVAIMGGLAAATILTILFVPA
                     LYTFWEGFTTRIMQSRRNKL"
     gene            complement(6435..7514)
                     /locus_tag="VNZ84_02530"
     CDS             complement(6435..7514)
                     /locus_tag="VNZ84_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU65856.1"
                     /translation="MNKTVISSLCLAAIVSACHQPPEETAAPRPVWVMSVGSTTSSIE
                     ASYTGEVKSRYESNIGFRIAGKIISRAVNVGDAVKKGQLIAQLDPNDSRLNALAAGAE
                     VQTAQANLALAQAELARRQQLYSQKFISKSALDSYETQVKTTQARLEQAKSQAAVSQH
                     QTGYTQLVADRSGVIGMIQAEPGQVVSAGQTIAQIYDLQTLEILIAVPESRIDSLHVG
                     DSAHITLSDLPQSYPGRIREISPAANSQTHAFDLRIQLLDADPRIKLGMTAQVRFKPQ
                     QTTSAIIVPTTAVTRQGDHASVWVIDAQHKAHARQVTTGALSEHGIEITSGLQAQDTI
                     ATIGIHTLTEGMQVQPVTPAPEVLR"
     gene            complement(7507..8145)
                     /locus_tag="VNZ84_02535"
     CDS             complement(7507..8145)
                     /locus_tag="VNZ84_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU65857.1"
                     /translation="MPPKVKTDAEKQAVRTAIMDAARELFIHQGVQAVTMRAVAQRIG
                     YSATSLFLYFTDKEQLLRAVVDADVIKLAQALKSTLSIADPLQRFLQFGQHYVQFALT
                     NPNHYRMMFMTQHPPCDPALSSIEQNNPEQDAYSMLIGVVTSAWHAGVFKPSLTDPVL
                     IAQTIWAAMHGLCALHINLADDAWINWRSLESRIQAMIEMCLHGLMKEPTHE"
     gene            8392..8796
                     /locus_tag="VNZ84_02540"
     CDS             8392..8796
                     /locus_tag="VNZ84_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWU65858.1"
                     /translation="MNVRQRWVSFLGRVLLSLIFIISGIAKVMDPAGTIAYIESAHLP
                     LPQVAYVVALIVELGLGLALLVGFKAQLAAAGISLFTFVTALMFHSNMADPVQVIMFL
                     KNITIIGGLLVVIAFGPGGYSVDGGKRVMLVE"
     gene            complement(8809..9690)
                     /locus_tag="VNZ84_02545"
     CDS             complement(8809..9690)
                     /locus_tag="VNZ84_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU65859.1"
                     /translation="MASIEEMHYFVEVVKHGNFTSAALALGISKQLVSRRIIALEARL
                     GVRLLLRTTRKLSPTETGKVFFQRAQQILQAIHDAELEISNRSDELRGLLKISVPLSY
                     ANLRLAPALTAFMQQHPLVEVWLDADNRHVDMVSEGYDMVIRVTDQPEEGMVARKLEN
                     AAIRYCCSPDYIARYGTPDSPQALGKHACLTHRTSEWLFTERDQIHRIPIHARLRSNH
                     GEVLREAALAGLGITGLPAFYVLDDLHSGRLVEVLAAFAMHQGAIYAMYPYHKQVSAN
                     TLALIEHLRSWFQSAQS"
     gene            9846..10235
                     /locus_tag="VNZ84_02550"
     CDS             9846..10235
                     /locus_tag="VNZ84_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWU65860.1"
                     /translation="MNAFQSAASVAGRVLLSLIFIISGLGKVANPAGTIAYIQSVGAP
                     LPEVAYAIAVFVELVLGIALLVGFKARWAAAGIAVFTIAAAFLFHNNMADQMQQIMFL
                     KNFTIAGGLLMVVAFGAGAFSVDNRRR"
     gene            10251..11021
                     /locus_tag="VNZ84_02555"
     CDS             10251..11021
                     /locus_tag="VNZ84_02555"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HWU65861.1"
                     /translation="MTTLFVSHGAPMLALEPGHTGQVLADIAARLPRPDAILVVSAHW
                     DTRLPTVSLASQPETIHDFAGFPAALYAQQYPAPGAPQLAQQVAALLQSHEIPVHIHP
                     DRGLDHGAWVPLMLMYPQADIPVTQLSIQSRATPAQQFALGQALRELHSQNVLILASG
                     AITHNLADFFTGDRDAESLSYVTAFADWMAARIQTQQWQSLLNYRMECPQGRQAHPTE
                     DHLMPLFVAAGSAQGAVERFTPEHTYGILAMDAYLWTA"
     gene            complement(11037..12395)
                     /locus_tag="VNZ84_02560"
     CDS             complement(11037..12395)
                     /locus_tag="VNZ84_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWU65862.1"
                     /translation="MSTPQHTILAVDDEPHMRRLLEISLRQAGYRALSAGHGREALEL
                     VQQQQIDLVVSDLHMPGMSGLQLLENLRKQYERLPFIMVTAQGEIKTAVEAMKLGASD
                     YILRPFELETLEIAIQKALAVNRIQLENSFFKESSQPPVTGLIGESDSMQSLKKMIQQ
                     VAPERATVFIVGETGTGKELVAKALHDASPRTSELFVAVNCAAIPADILESELFGHEK
                     GAFTGAVKERVGKFELANGGTLFLDEITEMPIQLQSKLLRVLQENVVEKLGGNRQISL
                     DVRVVAATNRDPMQAVKEGKLREDLYYRLNVIQLQVPPLRERGQDVSLLAEYFLGKRQ
                     TKLSESTRLCLQQYRWPGNVRELENILERAAILAGNQTIQPQHLPADISQQIAPSTAG
                     ANSDNGDSNDLSIPRATEAIERRLILQALDACQGNKTKAAKLLEISERSLWNKLNQYG
                     LR"
     gene            complement(12398..14026)
                     /locus_tag="VNZ84_02565"
     CDS             complement(12398..14026)
                     /locus_tag="VNZ84_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWU65863.1"
                     /translation="MLLIAFLLTGLLPALMVSVLSFYQAKYALRSEIQHDLQTTGKAV
                     AEHVDSVMNERVQNVRSWSQLAIMQDLFIGDIDKRLSIFLAETRQSYAEQYISLDVVD
                     LQGHIIASSRPANIGQPLHTPPVWMSFRDNGQPITIFRLDHQQLMIAAPVVSHNTWQP
                     MGQLIATFNWQVIDNLLDHAAQHSTELALLDHRQATLAQSRHWADQKHSLHATATVTS
                     HLPLDGWQIHLNKDHDVAVAPVHRLGNIFIALLGMILVASLLLVRPIAQNISRPLEAL
                     TRYVQRFRQSGVTPPESSGPAEVQSLHQAFTQMAADLEASEQQLTRAAKLAVVGEMAA
                     AMSHEVRTPLGIMRSSADVLLREPQLSEDGREVLGFIISETERLNKLVTALIDSARPR
                     PPNKIPLDLQAHLQHVVAMLQKQAQAKQIELSLQAPQPVIVAADQDQMTQVLVNLLMN
                     AIQILPEGGHISLHLGADAQEAHIVVADDGPGVPPAQQSHLFEAFFTQRAGGVGLGLA
                     VVKQIIEAHDGTISYSTSPWQGAQFNLSLPLVTA"
     gene            14119..15141
                     /locus_tag="VNZ84_02570"
     CDS             14119..15141
                     /locus_tag="VNZ84_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65864.1"
                     /translation="MLSSLLRVTLSVISLCMLCKSAIAEEVDAFWNAFHVGGYSSMDM
                     RLPRTEAAQLKWNQVSMIVTWDQGGRVKFFSELELENPLSYDHHQGVNTKQSYLDLER
                     FYFDYNLNEKANVRVGRFLTPAGRWNQLHAPPLVWTASRPLVTSRFFPSSINGAMLFG
                     SLPVQHMDMEYQVYAEALKDQHQDGDEIIFREVRGARVAFNNILGLSGSNTGLNTLGL
                     NVMSYQKDYRGSPTYHLFGLDFLLELNRWEFSGEVFERKTANGDDGGRGAYLQSAYAL
                     GNEWYWITRLETLHEDDSKDADRWVLGVTKRVKRNQLLKFEFVGGSGEYQDVPRGFVT
                     SFAVMF"
     gene            15162..15641
                     /locus_tag="VNZ84_02575"
     CDS             15162..15641
                     /locus_tag="VNZ84_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65865.1"
                     /translation="MLVSWACGLLIASLQLGHAAEDNRIIAVIVPVQANLAPDVHADD
                     LGLIFWRKKLYAAHGQALHPVNLHSEHPLRLRFSQQVLHSSPKSQINYWNGLYFHGVQ
                     PPFTVQSEEAMIRYVAETDGAIGYIDACHVDDRIKPVFWLTPTKLVSEPPALRCDGN"
     gene            complement(15638..17317)
                     /locus_tag="VNZ84_02580"
     CDS             complement(15638..17317)
                     /locus_tag="VNZ84_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HWU65866.1"
                     /translation="MNIVATLTRTVRALFSPQTCLIWGVSLWLMLTGNHALFEHLLNL
                     YPWNAHNAGFIISISLFFTFTITLWLMLLSHGKSARWVLSITVVSAAWAAFYMDEFGV
                     IIDHVMLDNAAKTDTAEIMGLLSWGFFIKMMLLGVIPAWVLIKLVPARFYQRDAFKIR
                     MTQISLLLIGMILVVIPCSADYATFIREHKMVRMYANPTFFHYSLVKWTSKKLFTRSI
                     TGLQKTAIDAVNVEADQHHELIIMVVGETARFDRFSLNGYARETNPRLSKEDVVSFKQ
                     VTSCGTSTGVSVPCMFSVLTRKQYDEDKALHMENALDVLSHKGIKVLWRDNNSDSKGV
                     ALRVPYEDFRSPAHNPVCDDNECRDVGMLSGLDKFVEKNKDHDILIVLHQMGNHGPEY
                     YRRYPDAFKKFTPICKTGRLSECTQQEVDNSYDNAILYTDYFLSEVIQFLKKYDDKRE
                     TAMLYVADHGESLGEHGIYLHAAPYMIAPKEQTHVPSILWVGQHFDYQLSQVRPYENY
                     PLSHDDVFCTLLTAYELKSETCHAEFGWLAQNRELQEEQRQHLHPLKTNPH"
     gene            complement(17314..17958)
                     /locus_tag="VNZ84_02585"
     CDS             complement(17314..17958)
                     /locus_tag="VNZ84_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWU65867.1"
                     /translation="MIAAIGIVWMANATSIDLSLADRQFDFASQRFSLKHDFFYETVM
                     HIYAKHLLTVLWLGLLFLAWAPVKLIGNRLSPATRSQLRWIVFLALLNAIFVSCLKHQ
                     MPHACPWDVTRYGGNLPWAPTFTPHPAMEAGHCFPAGHATSGVWLAALCLLWLPDAPR
                     KALLVAVAGLSVGLLLGWSQQIRGAHFLSHTLTSLWLMCGWLLLLFTFSKSRIS"
     gene            18404..19045
                     /locus_tag="VNZ84_02590"
     CDS             18404..19045
                     /locus_tag="VNZ84_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HWU65868.1"
                     /translation="MLQAEPNTTFYHASSAYELRNFAQTKRYQVDEIISHMVELDSPC
                     RREVESFIAAVFKQVYGAEVSHFMPQLVALRDASGILMAAFGLHNAALGDLFLEQYLD
                     EPIEQLLSRQLEREISRAEITYFGNLAVANPRNAGVLIAHVIQHSLNMNLPWGVATAH
                     HSLQNGLIKGGVDLFPLAMANPERLSPEERAKWGSYYKHTPQIVAIRGVADPV"
     gene            19056..20549
                     /locus_tag="VNZ84_02595"
     CDS             19056..20549
                     /locus_tag="VNZ84_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWU65869.1"
                     /translation="MILQELQAQAESHPHSVALQGQAVQLTYGELAVEIRRWASALTG
                     LAPAASVALALDNGPAWIVLDLAILAAGWTNVPIPAFFSATQKQHAIQDAGVSLVLTD
                     ALVVWQGLYPQGEVVAEWQIAGKAVTVLRIHASDSASAAQKITYTSGTTGAPKGVCLS
                     SQVMWQVAHSIQAWTQPSQADTHFCVLPLATLLENVAGVYTSLMSGATVVVYPCHEVG
                     FSGSQFDIRRLHQGLQHSQATTAILIPELLRALVAYLQAGHAPLTELRFIAVGGAKVA
                     AELIQQAHFLGLPVYEGYGLSECASVVTLNTPQSQKPGSIGKPLPHVQLRVDYTGELW
                     VKGALYLGYMQAQGGTVMPTLDEDGYLATGDLAYQDDEGYWYLSGRKKNMFITSFGRN
                     VSPEWVESALLHQPAISQVCVFGEARPFNVAIAVPQPEVSIEQVAQAIAQANQSLPDY
                     AQVGDVVFASQTFSPANGQLTANGRLKRDMIAAAYAAEINAIYEGTL"
     gene            20546..21238
                     /locus_tag="VNZ84_02600"
     CDS             20546..21238
                     /locus_tag="VNZ84_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWU65870.1"
                     /translation="MSFYQKLLSATEQERHTLMSLPLITQGAQGNISLALYRAFLTQA
                     YHHVKHTTPLLMACGGRLSGEYEWLRTAIGEYIEEEMGHQEWVLNDIEACGGNKEAVR
                     NSLSEEHAACHATEVMVAYAYDTIYRVNPLGFFGMVLVLEGTSTAVATQAGEKIMQAL
                     NLPKKAFSYLFSHGSLDISHVSFYESLMNQVTKPEDQQAIIHAAKRFYRLYGDIFNYV
                     ATLDQHDNVRMG"
     gene            21242..22033
                     /locus_tag="VNZ84_02605"
     CDS             21242..22033
                     /locus_tag="VNZ84_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU65871.1"
                     /translation="MELRDLHVVLTGATGGLGATLSLALSARGAVVCLVGRDPVKLHA
                     LQKRIVAQGGRSHIVVADLAQPNMAGQVLKQSLREMGHVDLLINNAGIIEFTEFAQQS
                     EVSIDNMVHTNVTATLVLSRTFVEYFLQRGRGHLAFIGSIFGSLGFPHFATYCASKFA
                     LHGFSQALRRELVDTNIGITYVAPRAIATPMNDERTSAMWQASGQAVDQPQVVADKIV
                     RALVKDQQELFIGQPQSFFAWLNGLAPKLVNLGLKQQTGLAKKFL"
     gene            complement(22048..22992)
                     /locus_tag="VNZ84_02610"
     CDS             complement(22048..22992)
                     /locus_tag="VNZ84_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HWU65872.1"
                     /translation="MKHSWVVVMLSKIKLSCLSVAFSFVTFCSPLCAETFELTQVADG
                     IYVHHGKHLDIDDGYQGDICNLGVIIGRDAIAVIDTGGSKHTGEQLLQAIKAISPLPV
                     RYVINTHVHPDHTYGNAAFKNGETHFVGHEKLANTMLLRQEQYEKLNARLLGEAGTTV
                     TPDMAVHGQQTLDLGKRVLQLQAQSVAHTHTDLTVLDTNTNTLFAGDLLFAQRTPVVE
                     GDIKGLISALEHLSQSHYTLIVPGHGLESTDQKSIIGNELDYLNLLLSDVRSSIKQGI
                     SMEQTMDTAAASERDKWQLFDIANRRNVNVIYPQLEWE"
     gene            23059..23655
                     /locus_tag="VNZ84_02615"
     CDS             23059..23655
                     /locus_tag="VNZ84_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HWU65873.1"
                     /translation="MSSTQRYTKVAIILHWLIALAIIAMFALGWYMGELPKDGPKQTA
                     FDLFDLGIYTWQMDEPASARTLYFNLHKSLGFSLLWLILFRVYWRISHTPPAMLGSYS
                     DLERKVATGAHHLLYLLMVAMPVSGVLMTLYSKYGLKWFGLEVFGGLDNADLRDLFKE
                     SHELFANILLVIFIVHVAGALKHRFIDKDQTMSRMSLK"
BASE COUNT         5307 a         5786 c         6614 g         5957 t
ORIGIN      
        1 cactttcaga gagcaattga tgagattgat aaatccatcg atcatctgca aaaaactaag
       61 gatgcattgc taagtaccag ccggaacctt cgtttagcca atgataaagc acaagatgtg
      121 actatcaaga agttgactaa aggtaatccg acgatggctg aaaagtttga agggttgaag
      181 cgataagcaa ccgtcaacgc taactccagc gatgacatag ttttctaata agtgtgattt
      241 agagcagtct ggtgccaagc ggaacttcat gttctggcac acacaatgtc actcttcctt
      301 ctgcatcatg aaaacccgtg accaggcatt ctgacatcaa tggcccaatt tgtttgggcg
      361 gaaaattaac cacggcgatc accagtttgc cgatcaggtt ctctggcgtg tagtgcgctg
      421 taatctgggc gctggattta cggataccga tctcggggcc aaagtcgatg tgtagtttgt
      481 aggctggttt gcgcgcttct gcaaatacct ctgccgaaat cacgcggcca acgcgtaatt
      541 caactttggt gaagtcattc cattcgattg gattcatatg cccagctttc taaagtgacc
      601 gggcattatc gcagataatt aatcttgaat tatcgaatca gtgccgacac tgctttgaac
      661 tgcgcatgcg tggcgccttg catgggctca aatagcatag actcggtatt ggtgataatg
      721 cagcctgcgc tgcgcattct tgctatggcg ttggtgcggt tggcttctgt gcgggagatg
      781 acggcgtctt ctacgataaa tacttgtttg ccagcggcga gtgcatcgag cgctgtttgt
      841 agtacgcaaa tatgcgtttc cagtccgaat aacaccactt gcggtgcaac gctgatggag
      901 gattcttgca aggcgggttc atgccaggca gagaaggcga gtttggctgt gcgcggggta
      961 tattctggca acagggcttg cagttcgggt aaggtttcgc caagcttttc cgggtactgc
     1021 tcggtaaccc agagcgggat attcagtaat tgcgcgcctt gcagcaagcg ttggatattt
     1081 ttgagcaccg ttgccatggt ggcttctggc atgacggaac agaggcgcgt ttgtacatca
     1141 atcaacatcc aaacggcatc tgtgctggca atgacctgat tcatgctggg tgacctctcc
     1201 taatgaataa caaaccgtgt gtagtggctt gcctagcgct tggcgagttg ctgttcgata
     1261 tcctgtaaat cggcttcgga cacaatgccg ctggcggttt ttaaacgaat aatgttaacc
     1321 aggtaattat agcgggcctg caataagtcg cggttcgcgc tgtagtattg ctgctgtgca
     1381 ttgagcacat ctacgctggt gcgtacgccg acttcataac cgagttgggt ggagtcgagc
     1441 tggctctggc tacttttgag cgcttgctcc agggctttga cctgcgcgat gctggtgctc
     1501 aggttaaggt aggcgcgctg tgtttcgagt gatgtttggc gcaaggcgtt ttgcaagtca
     1561 tccagcgctt tttgcttgtt atacgccgcc tggcgtacgc gcgaagtgac ggcgccgccc
     1621 tgatagagcg gcacggaaac ctctacgcct atgctggcgc ttttaaggtc gccaccaaaa
     1681 ccgtactggc cgccattggt atagctgtcc tggtagttcg ccacggcatc aacggtgggg
     1741 taatggccgg cgcgtgcaat ttcgatatct ttttcgttca cctgcaccat ttcctgctgg
     1801 atctggatat tcaggttact ggcattggtt acctcttgcc attcctgcat gggtttcagg
     1861 ctgggagtga tctggatatc tgcgcgcacg ctggcaagtt gttcaggcag tttgccggta
     1921 atggcttgca agctgcgttt ggcaatttca atctcattaa aggcagatag ctcttgcgcc
     1981 gcgatcaggt cgtagcgggc ctgcgcttca ttaaagtcgg taatggtggc ggtgccgaca
     2041 tcaaaattgg ccttggcttg ctgcaattgg ctttggatgg cttttttctg cgcctcaatc
     2101 agcgtcatgc ggtcttgcgc cagcaacaca tcaaagtagg cttgcgtggt gcgtagcatt
     2161 aggttttgct gagccaggtg atattgcttg tccgcaatgc tcacttgtac cttgctttgg
     2221 tcgatgcgct ccaggttttc tttgcggaaa atcggttgcc tggcttgcag ctgcgcctgg
     2281 taaccttcat aagtattgcg tccgccgggg aaaggtgcct ggctaccgac aatcttcaaa
     2341 tctgtgcggt tggcattcgc gcctgcggta aagtttactg aaggacggta acctgccttg
     2401 gcctgttcta tgatctcctg tgctgccagg ttggcactcc ccgcggaagc cagcaccggg
     2461 tcattggctt tggcttcctg gtaaatgtcg accaggcttt gcgacgctgc ccatgtcgtg
     2521 accggcagcc acaggctgct ggctagtaca atcaagcggg gcaggtgatt caacgacatg
     2581 gcaggtgagt tctagaatga gaaggcttgg gcttgcggcg cgttttgcat ttctgtcagg
     2641 caggtttcaa acagcacgtc ctgtttgtag ctgtgctctg ccaggcgctg gatcaatgtg
     2701 gcctgcatca ccggtgcctg gccgaggacg atcagcatgc ggccaccaat gctcagctgg
     2761 tagcgcacac cttcaggctc taccggactg gaggcgctga agataatggc gtcataaggc
     2821 gcttgtgact catagccatt gtggccatca atttcaatca cttgcacatt ctggatactt
     2881 tgcttggtca ggttttggct ggccagggca cacaactcag ggaagacttc cagggccgtc
     2941 acttgtgcgc agtgttgcgc catcaatgcg gccaggtaac cactgcctgc gcctatcagt
     3001 aacactttgt ctttggcttg caattgcagg gcttgtaaaa agcggccttc aatttttggg
     3061 ctgagcattt gctgcccgtg cgccagcggg atttcaatat cggcaaacgc caggccacgg
     3121 tacgcttctg gtacaaaagc ttcgcgtggc atattgttta aggtcgccaa tacttgcggg
     3181 tccaacacct cccaggtgcg gatttgttgc tcaatcatgt taaaacgtgc ggttgcctga
     3241 tctgtcatgt cttgaatccc agttgattat tgataagccg tattatagtt tattgcgcct
     3301 tgattgcatg atccgggtcg tgaatccttc ccagaaggta tacagtgccg ggacaaataa
     3361 aatggtcagg atggtggctg cggccagccc gcccatgatc gccacagcca tcggcccgaa
     3421 aaacacgctg cgtgtcagcg gaatcatggc caggatggcc gctgcggcgg tcagtacaat
     3481 aggccggaag cgacgcacgg tggattgcac cacggcttgt tccagcgaag caccttcggc
     3541 aatatcctgc ttgatctggt caaccaggat gacggagttg cgcatgatca tgcctgacag
     3601 cgcgatactg cccagcatgg caacaaagcc aaacggcata ttgaacatca gcagggcgaa
     3661 ggtgatgcct atcatgccca atggcgcggt cagcacgacc aggatgacgt tggaaatact
     3721 gcgcaattgc agcatcagca gggtgaatac ggcggctaaa aacagcggta cccctttagc
     3781 cactgaggtg gcacccttgg cggactcctc aatcgcaccg ccggtttcta ccgtcacgcc
     3841 tgcgggtagg tgctggagaa tcggctgtat cacttgctgg atttgtgccg acactaccgg
     3901 ggcctgcatg ctgccgcgca agtgcgcgcg caccgtgagt gagggtatgc ggttgcggcg
     3961 ccagatcacg ccttcttcaa aaccgtagtg gatgttggcc acctgagcca gcggtacacg
     4021 ctcgccgcgc tggcttagta cggtgagctg tgcaagttgg gagagttggg tgtgctcttg
     4081 ttgggtactg cgcatgacga ggtctatgcg ttcgttgcct tcccggaact cgctcaccac
     4141 ttgctcaaaa tattgggtgt tcagcaattg ggcgatgtct ggcgagttga cgcccagcat
     4201 gcgggctttg tgctggtcaa tgtcgacatg aatgactttg cttggctctt cccagccaag
     4261 ttgcacattg cgcaagtgcg ggttggcgcg catggcatcg gcaatcttat gcgccacttc
     4321 ccgaatcgcc tgcaggtttt gcccatcgac ccggaactgt accgggaagc ccacgggtgg
     4381 gccattttcc aggcgcagta ccgaggcgcg caactgtggg aagtcatgtt caaacaaggt
     4441 gagcaggtca ttacgcaggg cttcgcgctg ttccagggtg ttggtcaaga tcaccagttg
     4501 gccgaaagcc cggtgtggca gttgcacatc cagcggcagg tagtaacgcg gggcgccagc
     4561 gcccagataa gacaccacat tttctagttt gccatgggct tgttgccagc cttgcagcca
     4621 ttgctccagt tgcttgaccg cggtttcagt agactggtca ctagcatttt cggccaggcg
     4681 caggtcgacg atgatttcca gccgggtgga gtccgggaaa aactgttgtt gtaccttgcc
     4741 aaagctgagc atggcgacca caaacaaaat aatggtcgcg ataatcacgc tgcctttgta
     4801 gttgacgcac caggcgaccg tgctttgcag gcgacggtaa aaagaggttt cgtaaataga
     4861 ggcctggttg tgagcacttg cttcgttgtg agggtgcttg gcatctggta ataaatggta
     4921 gcccaggaat ggaatcacaa tcacggcagc gacccaggaa agggtgagtg caatcgcaga
     4981 gacctggaaa atggagcgtg tgtattcgcc ggtggaagac actgccgtgg caatcggcag
     5041 gaaacctgcc accgtgacca gggtgccggt cagcatgggc atggcggtgg aagtgaatgc
     5101 aaaggatgcc gcttgcatgc gctcccagcc ttgctccatt ttggcagcca tcatctccac
     5161 ggcaataatc gcgtcatcca ccagcaagcc gagggcaagg atcagggcgc ccagcgagat
     5221 tttgtgcagg ccaatgtcca gccagtccat aagcaggaaa gtgcctgcga gtacgatggg
     5281 aatagccacc gccaccacaa tgccggtgcg ccagcccagt gatagcaggc tcacggctag
     5341 cacaataatg agcgcctccg tcagtgagcg cacaaagtcg tgtacggagt gctgcacaat
     5401 gtcaggctgt gaggtgacca gttgcaggtt gacgcccagc ggcagctgtt tttgtacgct
     5461 ggtgatgatt tgctgcatag ccttgcccag ggcgatcacg tcgccgcctt gctgcatgct
     5521 gacaccaatc aggagcgcgg attggccgtt aaagtggatg cgttccgcag gcggctcggc
     5581 gtaaccgcgt ttgatggtgg cgatctcgcc cagggtcagg ttttggctgc ctgcgcggat
     5641 ggggaattgg cgcaggtctt ccagagtttg taagcgacca cttacgtcta gccggattct
     5701 ttccggacct gcatcaaaag tgccggaggc aatcacctta ttttgttgtt gcagtaactg
     5761 cgccagttgg gctggtgtga tgccgagaga agtcagcttc tggttggaaa tttctatgat
     5821 aattttttgt ggttgctcgc caaataaatc gactttttcc acatctggca gtttgagcag
     5881 gcgggcacgg acccattcgg cctgccgttt taattgctgc ggattcaggc cgtcggccgt
     5941 gatcgcgtac atggtgccat agacgtcgct gaactcatca ttaaaagtca ggctttgcag
     6001 gtcgctgggg aaagtgtggc ggatgtcgcc tatttttttg cgtacctggt accacagctc
     6061 gggaatcttt tccgagaagg tgttatccct gatcacaatg aacaccaccg attcgccggg
     6121 gcgtgagtag ctgctggtgt agtcaatgga agggatttcc tgcagctttt tctcaatttt
     6181 atcggtaatt tgctgttcga cttcctgggc cgaggcgcct ggccagctgc tacgtaccag
     6241 catcacctta aaggtaaatg gtgggtcttc ggactggcct aaatgggtgt aagcaaaggc
     6301 cccggccaat aaggtcagga gaataaaata cagcaccagc gcctggtttt tgagggccca
     6361 ggtggacagg ttgaaccggt ggtgagccgg ttgcccgtca atatgtgcat ctgaggtatg
     6421 ttctggcgcg ctcattagcg taacacctcg ggggctggcg tcaccggttg tacctgcatg
     6481 ccttcagtta gggtgtgtat gccgatggtg gcaattgtgt cctgtgcttg caaaccgcta
     6541 gtgatttcga tgccgtgctc gctgagtgcc cctgtggtca cctggcgtgc gtgtgcttta
     6601 tgttgcgcgt ctatgaccca gacgctggca tgatcgccct ggcgtgtcac tgcagtggtc
     6661 ggtacgatga tggcagaggt ggtttgctgt ggtttaaaac ggacctgggc cgtcatgccg
     6721 agcttgatgc gcgggtctgc atccagcaac tggatgcgca aatcaaaggc gtgggtctgg
     6781 ctgttggccg ctggcgagat ttcacgtatg cggcctggat aactttgcgg caggtcgctg
     6841 agagtaatat gggcgctgtc gcccacgtgc aggctgtcta tacgggattc cggcacggca
     6901 attaaaattt ccagggtctg caaatcataa atctgggcga tagtttggcc ggcggagacc
     6961 acttgccccg gttcagcctg aatcatgccg atcacaccgg agcggtcagc caccagctgg
     7021 gtataaccag tctgatgctg gctgaccgct gcctgggatt tggcttgttc caggcgggct
     7081 tgcgttgttt ttacctgggt ttcgtaacta tccagggctg attttgagat gaacttctgg
     7141 ctgtatagct gctgacggcg cgccagttct gcctgtgcca gtgccaggtt ggcttgggcg
     7201 gtttgcactt ctgcaccggc cgccaacgcg ttgaggcgcg agtcattggg gtccaactgg
     7261 gcaattaact ggcctttttt gactgcgtcg ccgacattga ccgcgcgtga aatgattttc
     7321 cccgcgatac ggaagccaat gttggattcg tagcgggact tcacttcccc tgtataactg
     7381 gcctcaatgg aggaggtcgt gctgccaacg gacatgaccc ataccgggcg tggtgcagcg
     7441 gtttcttctg gtggctggtg gcaggcactg acgattgcgg ccaggcacaa gctgctgatg
     7501 acagtcttat tcatgcgttg gttctttcat taatccatgc aggcacattt ctatcatagc
     7561 ctgaatgcgg gattccaagg aacgccagtt gatccaggca tcgtcggcga gattgatgtg
     7621 taatgcacac aagccatgca tggcggccca tatggtttgg gcaatgagga ccgggtcagt
     7681 cagggacggt ttgaacacgc cggcatgcca ggcgctggtg acgacaccga ttaacatgga
     7741 gtaggcgtcc tgctcaggat tgttttgctc aatgctggaa agcgccgggt cacatggtgg
     7801 atgttgggtc ataaacatca tgcggtaatg gttggggttg gtaagtgcaa attgcacata
     7861 gtgctgccca aactgtaaaa aacgctgcaa tgggtcagca atgctgaggg tcgattttaa
     7921 tgcttgcgcc aacttgatca catcggcatc taccacggcc ctgagcagtt gttccttatc
     7981 cgtgaagtaa agaaaaaggc tggttgccga atagcctatc cgttgcgcca cggcacgcat
     8041 ggtgaccgct tgtacgccct gatgaataaa aagctccctg gccgcgtcca taatcgcggt
     8101 gcgcacagct tgtttttcgg cgtcagtttt tacctttggg ggcatgcagg acaatcggta
     8161 taaattaata acaatgttaa cttaacactg ttatttttaa atgtcaaatc ttgctggtat
     8221 ttcttgcaag aatggtgcag agtaacaaat gtgatacacc ggtagtcaga attggggctg
     8281 agtcgctggg tagtcaatgc ggctgaacgt ttaccggcat gcgtcatcac gctgtcttca
     8341 cgccaggcag gcggaaggca acgcgttcaa taacgacaag gagcgatcag tatgaatgta
     8401 cggcagcgtt gggtcagttt tttgggacgt gttttgttat ctctgatttt tatcattagc
     8461 ggcatcgcca aggttatgga tccggcaggg accattgcct atatcgagag tgcgcacttg
     8521 ccgctgccgc aggtggctta tgtggttgcg ctgattgtag agcttggact gggcctggcg
     8581 ttactggtcg ggttcaaggc gcaactggct gcggcgggaa tctcgctatt tacctttgtg
     8641 acggcattga tgtttcacag caatatggcg gatccagtgc aggtcattat gtttctcaaa
     8701 aacattacca tcattggcgg gctgttggtc gtgattgcat ttggtcctgg cggttacagc
     8761 gtggatggcg gtaagcgggt tatgctggtg gaataatcac tagacgcctc aggattgcgc
     8821 agactggaac cagctgcgca ggtgttcgat cagcgccagg gtgttggctg atacctgctt
     8881 gtgataggga tacatggcgt aaatcgctcc ctggtgcatg gcgaacgctg ccaatacttc
     8941 caccagacgc ccggagtgca aatcatccag cacataaaag gccggtagcc cggttatccc
     9001 caggccagcc agggccgcct cgcgcaatac ctctccatga ttggatctta acctggcatg
     9061 aatggggata cggtgtatct ggtcgcgttc ggtaaatagc cattcactgg tgcggtgtgt
     9121 caggcaggca tgtttgccaa gcgcctgcgg tgaatctggt gtgccatagc gggcaatata
     9181 atccgggctg caacagtaac ggatggctgc attttccaat ttacgtgcga ccattccctc
     9241 ttcgggctgg tccgtcacgc ggatcaccat atcatagccc tcactcacca tgtcgacatg
     9301 ccggttgtct gcatccagcc agacttcgac caaaggatgt tgttgcataa acgcagtcag
     9361 ggccggggca agccggaggt tggcatagga caggggtaca gaaatcttga gtaagccccg
     9421 taattcgtct gagcggttgc tgatttcgag ttcggcgtca tgaatggctt gaagaatctg
     9481 ttgcgcgcgc tggaaaaaga ctttgccggt ttcagtcggg gacagtttgc gggtggtgcg
     9541 caacagtaag cgcacaccca ggcgagcctc aagggcaata atgcggcggc tcaccaattg
     9601 cttggagata cctaatgcaa gggctgcgct ggtgaagttg ccgtgtttca ccacttctac
     9661 aaagtagtgc atttcttcga tggatgccat tgattgtcaa cctatgtgag acagtcttac
     9721 cattattggc atgattatca tttttttatt tcaggaatac aatgcctcat cagctgaatt
     9781 gtctcaggga atttagatgc ctggctgata tggcatcaca caaccacaca taaggagtca
     9841 tcgttatgaa tgcatttcaa tccgccgcca gcgtcgctgg ccgtgttctg ttatcactga
     9901 ttttcattat cagcggcctg ggtaaggttg ctaaccctgc cggtaccatt gcttatatcc
     9961 agagtgtagg cgcaccattg cctgaagtag cttatgcgat tgccgtattt gtcgaactgg
    10021 tattggggat tgccttgctg gttggattta aagcccgctg ggccgccgcc ggtattgccg
    10081 tgtttaccat tgccgctgcc tttttgttcc acaacaatat ggctgatcaa atgcagcaga
    10141 tcatgttcct gaaaaatttc accatcgcag gtggcttgtt gatggtggtg gcgtttggcg
    10201 caggcgcatt cagcgtggat aaccgtcgcc gctaattgga gtagccggga atgacgaccc
    10261 tgtttgtttc acatggcgca cccatgctgg cccttgagcc cggtcacacc gggcaggtgc
    10321 tggcggatat tgccgcacgg ctgccgcggc cagatgccat tctggtggtg tctgctcatt
    10381 gggatacccg gctgccgaca gtgagcctgg ccagccaacc ggaaaccatc catgattttg
    10441 ccggatttcc agcggccttg tatgcgcaac aatatccagc ccctggcgcg ccacaactgg
    10501 cgcagcaggt agctgcattg ttgcagtcac atgaaattcc cgtgcatata catcccgacc
    10561 gcgggttgga tcatggggcg tgggtgccgt tgatgctgat gtatccgcag gccgatatcc
    10621 cggtcacgca attgtccatt caaagccggg ctacaccagc ccagcagttt gccttggggc
    10681 aggctttgcg ggagctgcac tcacaaaatg ttttgatact ggcctctggc gcgatcacgc
    10741 acaatttggc agattttttt accggcgacc gggatgcgga atcattatct tatgtcaccg
    10801 cgtttgcgga ctggatggcg gcacgtattc agacgcaaca atggcaaagc ctgctcaact
    10861 accgcatgga gtgcccacag gggaggcagg cccacccgac tgaagatcac ttgatgccct
    10921 tgttcgtggc tgctggcagt gcacaggggg cggtcgagcg gtttacacca gagcatactt
    10981 acggcatcct ggccatggat gcttatttgt ggacagcgtg atcgggtagc ttggctctag
    11041 cgtaagccgt actggtttaa cttgttccat agtgagcgct cgctgatttc cagcagcttg
    11101 gcggccttgg ttttattacc ctggcaggca tccagtgcct gtaaaatcag acggcgctca
    11161 atcgcttctg tggcacgtgg aatggataag tcattgctat ctccgttgtc actgtttgcc
    11221 ccggcggtag atggtgcaat ttgctgtgaa atatccgcgg gcaaatgctg cggctgtatg
    11281 gtctgattgc cagccaggat ggcagcccgc tccaggatgt tttccagttc gcgtacgtta
    11341 ccgggccagc gatattgttg caggcataag cgtgtactct cactgagttt ggtttggcgt
    11401 ttaccaagaa aatattcggc cagcaggctg acgtcctgtc cacgctcgcg caatggcggt
    11461 acctgcagtt ggatcacatt caggcggtag tacaagtctt cgcgcaactt gccttctttg
    11521 accgcttgca tgggatcacg gttggtcgcg gcgaccacgc ggacatccag cgaaatttgc
    11581 cgattacccc ccagtttctc aaccacgttc tcctgtaata cccgcagcaa tttggattgc
    11641 agttggatgg gcatttcagt gatttcgtcc aggaacaaag tgccgccatt ggcgagttca
    11701 aatttaccga cacgctcttt gactgcgccg gtaaatgcac ccttctcgtg gccgaataat
    11761 tctgactcca ggatgtcggc cggtatcgct gcgcaattga ctgccacgaa caattcagat
    11821 gtacgtggag aagcatcatg caaggctttg gcgaccagtt ccttacccgt accggtttct
    11881 ccgacaataa acactgtcgc gcgctcaggc gcaacttgct ggatcatctt cttgagtgat
    11941 tgcatggaat cactctcgcc aatcagacct gtcactggcg gctggctgga ctccttgaaa
    12001 aaagagtttt ccagctggat gcgattcacg gccaacgcct tctgaatggc aatttccagt
    12061 gtttccagct caaatgggcg caaaatataa tcactcgcac ccagcttcat cgcttcgacg
    12121 gctgttttaa tctcgccttg tgccgtcacc ataatgaagg gcagccgctc gtattgcttg
    12181 cgtaaatttt ccagcaactg caaaccgctc atgccgggca tgtgcaggtc actcacaacc
    12241 aggtcaattt gttgttgctg caccagctcc agtgcttccc ggccatgtcc tgcagagagg
    12301 gcgcggtaac cggcttggcg caagctgatt tcgagcagcc tacgcatatg gggttcatca
    12361 tcaacggcca ggatggtatg ttgcggagtc gacatggtta tgcagtgacc aaaggaagtg
    12421 ataaattaaa ttgggcacct tgccaagggc tggtgctata actgattgta ccatcatggg
    12481 cttcgatgat ttgtttcacg acagccaacc ccaggcctac gccgcctgca cgttgcgtaa
    12541 agaaggcttc gaataaatgg ctctgttgcg cgggtggtac accgggacca tcatctgcga
    12601 ccacaatatg ggcttcttgg gcatccgccc ccagatgcag cgaaatgtgg ccaccctctg
    12661 gcaatatctg gatcgcattc atgagcaggt taaccagcac ctgggtcatt tggtcctggt
    12721 cggctgcgac gatcaccggc tgtggtgctt gcaatgacaa ctcgatctgt tttgcctgtg
    12781 cctgtttctg caacatggca accacatgct gcaagtgagc ctgcaggtcc agtgggattt
    12841 tattgggggg gcgcggccgt gcagagtcaa tcaaggcggt gaccagctta ttcaggcgct
    12901 ctgtttcaga aataataaag cccagtactt cgcggccatc ttcactcagt tgaggttccc
    12961 ttaataacac atctgccgaa gaccgcataa tgcctagcgg cgtgcgaact tcatggctca
    13021 tggcggctgc catttcacct acgactgcca gtttggcggc gcgggtcagt tgttgttcgg
    13081 acgcttcaag gtctgccgcc atttgggtaa atgcttgatg caaggattgc acttcagctg
    13141 gtccgctgga ttcggggggt gtcacgcctg actggcggaa acgctgtaca tagcgggtca
    13201 atgcttccag ggggcggctg atattctgtg caataggccg taccagtaac agggaagcta
    13261 ccaggatcat gccaagcagg gcaataaaaa tattgcctag ccggtgtacc ggggccacag
    13321 ccacatcgtg atccttattc aagtggattt gccagccatc cagcggcagg tggctggtca
    13381 ccgtggcagt cgcatgcagg ctatgcttct ggtcagccca gtgcctggat tgggccagtg
    13441 ttgcctgtct atggtcgagc aaggccaatt cggtactgtg ctgggccgca tgatcaagca
    13501 ggttatctat cacctgccaa ttaaaagtgg cgatcagttg ccccatgggt tgccaggtat
    13561 tgtgcgacac cactggcgcc gcaatcatga gttgctgatg gtcaagcctg aaaatggtaa
    13621 tgggttggcc attgtcacga aaagacatcc agaccggtgg tgtatgtaat ggttgcccga
    13681 tgttggctgg ccggctggag gcaatgatgt gcccttgcag gtctaccacg tccaggctga
    13741 tatattgttc tgcatagctt tgccgtgtct ctgccagaaa aatggacaac cgtttatcaa
    13801 tgtcgccaat gaacaagtct tgcatgatgg caagctggct ccagcttcgc acgttttgta
    13861 cgcgctcatt catgacgcta tcgacgtgtt ctgccactgc cttacccgtg gtttgcaagt
    13921 catgctggat ttcactgcgc aatgcgtatt tggcctggta aaaactgagc acactcacca
    13981 tgagtgccgg aagtagcccg gtcagcaaaa atgcgattaa aagcattttg cggatggtaa
    14041 gtggcgaatg aaagcgcatg agatgtgaga aaatacgggt taaatggttt ttgccggaat
    14101 acgcatcatg gatcagatat gttaagcagt ttactcaggg tgacgctcag tgtaatcagc
    14161 ctgtgtatgc tatgcaagtc agccatagca gaagaggttg atgcattctg gaatgccttt
    14221 catgtcggcg gctacagcag catggatatg cgcctgccac gcacagaagc ggcacagctt
    14281 aaatggaatc aggtcagcat gatcgtgacc tgggatcagg gtggacgcgt caagttcttt
    14341 agcgagctgg aacttgaaaa tcccctgtct tacgaccatc atcagggcgt caataccaag
    14401 caaagttatc ttgaccttga gcgattttat tttgactata acctcaatga aaaggcgaat
    14461 gtccgcgttg gccgcttcct gacaccggct ggacgctgga accagttgca tgcccctccc
    14521 ttagtctgga ctgccagccg cccgctggtc acttcccgct tttttccttc cagtatcaat
    14581 ggggcgatgc tatttggcag tctgccggtg caacacatgg atatggagta ccaggtctac
    14641 gcggaggcac tcaaagacca gcaccaggat ggggatgaga ttatttttcg tgaggtcagg
    14701 ggcgcgcgcg ttgcgttcaa caatatcctt ggcctgagcg gcagtaatac cgggctcaat
    14761 acattgggct tgaatgtcat gagctaccaa aaggactatc gcggctcacc cacttatcat
    14821 ttgtttggcc tggacttttt actggagctc aaccgctggg agttcagtgg cgaggtgttt
    14881 gaacgcaaaa ccgcaaacgg tgatgatggc ggcagaggcg cctatttaca gagtgcctac
    14941 gctttaggca acgaatggta ctggattacg cggcttgaaa ccttgcatga ggatgatagt
    15001 aaggatgcag atcgctgggt gctgggtgtc accaaacggg tcaagcgtaa ccagttgctc
    15061 aagtttgagt ttgtcggggg cagcggtgag taccaagatg tgccgcgtgg ttttgtgacc
    15121 tcttttgcgg tgatgtttta atgcgtgcac agtattcaaa aatgcttgta agctgggcat
    15181 gcggcctgtt gatcgccagc ctgcagttag gacatgctgc tgaggataac cgcattatcg
    15241 ctgtcatcgt gccggtgcag gcaaacctgg cgccggatgt gcatgcagac gatctggggc
    15301 tgatcttctg gcgtaaaaaa ctgtatgcag cacacggcca ggccttgcat ccggtgaatc
    15361 tgcatagcga gcatccattg cgcctgcggt tttcacagca agtgttgcac agtagcccga
    15421 aatcccagat taattactgg aacgggctct actttcatgg ggtgcaaccc ccgtttaccg
    15481 tgcagtcaga ggaggccatg atacgttacg tggctgagac cgatggggcc atcggctata
    15541 tagatgcctg ccacgtagat gaccgcatca agccagtgtt ctggctcacc cccactaagc
    15601 ttgtcagcga accgcccgca ttgcgctgtg acggcaatta atgtggattg gtttttagcg
    15661 ggtgcaaatg ctggcgctgt tcttcctgta actcacggtt ttgagccagc cagccaaatt
    15721 ctgcatggca ggtttctgac ttaagctcat aagcggtgag taaggtgcaa aacacatcat
    15781 catggctcaa tggatagttc tcgtagggcc tgacttgcga cagctggtaa tcaaagtgct
    15841 gccccaccca caggattgaa ggcacatggg tttgttcctt gggtgcaatc atataaggcg
    15901 cggcatgcag ataaatgcca tgctcgccca atgactcgcc gtggtccgcc acatatagca
    15961 tggcggtttc gcgtttatca tcatattttt tcaggaactg gatcacttct gataaaaagt
    16021 aatcggtata caaaatggca ttatcgtaag agttatccac ttcctgttgc gtgcattccg
    16081 agaggcggcc agttttgcaa atcggcgtga attttttaaa cgcgtctgga tagcggcggt
    16141 agtattccgg accatgatta cccatctggt gcagcacaat caggatgtca tggtccttat
    16201 ttttttccac aaacttgtcc agtccgctga gcatgccgac atcgcggcat tcgttgtcgt
    16261 cacacaccgg gttgtgtgca ggcgaccgga aatcctcata gggcactcgc aaggcgaccc
    16321 ctttggagtc agagttattg tcccgccata gcaccttgat gcctttatgg ctgagcacat
    16381 ccagggcgtt ttccatatgc aaagccttgt cttcgtcata ctgtttgcgt gtcaggactg
    16441 aaaacataca aggtacggaa acccccgttg aggtaccgca ggaagtcact tgtttaaaac
    16501 tcacgacatc ttccttgctc agcctggggt tggtctcgcg cgcatagcca ttcaatgaaa
    16561 aacggtcaaa gcgtgctgtt tcacccacga ccatgataat cagttcgtga tgctggtcag
    16621 cttctacatt cactgcatca atggcggttt tttgcagtcc ggtgatgctg cgcgtgaaca
    16681 gtttcttgga cgtccatttc accagtgagt aatggaaaaa tgttggattg gcatacatac
    16741 gcaccatttt atgttcgcga ataaaggtgg cgtaatccgc actgcaggga atcacgacca
    16801 gaatcatgcc gatgagtagc aggctaatct gcgtcattct gattttgaat gcgtcgcgct
    16861 ggtaaaaacg tgcgggcacc agcttgatca atacccaggc gggaatcacg cccaacagca
    16921 tcattttgat gaaaaatccc caggacagca ggcccatgat ttcagccgta tctgttttgg
    16981 cggcattgtc cagcatgaca tgatcaatga tcacaccgaa ctcatccatg taaaaagcag
    17041 cccaggccgc agagaccacg gtgatggaca atacccagcg tgctgacttg ccgtggctga
    17101 gcaacatcag ccatagggtg atggtaaaag taaaaaacag gctgatgctg atgatgaagc
    17161 cagcattgtg tgcattccaa ggatacagat tcagtaaatg ctcaaacagg gcatggttgc
    17221 cggtgagcat tagccataga ctcacacccc agatcaggca ggtttgtggg ctaaaaagcg
    17281 cacgtacggt gcgggtgagt gttgcaacaa tattcatgaa atacgactct tggaaaaagt
    17341 aaacagcagt aacagccagc cacacatcag ccagagagag gtcagtgtgt gtgataaaaa
    17401 gtgggcgccg cgtatttgtt gcgaccagcc caacagcaaa cccactgata gtccggcaac
    17461 cgccaccagt aacgctttgc gtggcgcgtc aggtaaccag agcaggcaca gtgcagctag
    17521 ccagaccccg ctggtagcgt gccctgccgg aaaacagtga ccggcttcca tcgcagggtg
    17581 aggggtaaat gtgggtgccc atggcaagtt tccgccataa cgtgtcacgt cccaggggca
    17641 ggcatgcggc atttggtgtt tcaggcagct gacaaagatg gcgtttagca gtgccagaaa
    17701 aacgatccag cgcaattgag agcgtgtggc tggagataac cggttaccaa tcaactttac
    17761 cggggcccat gccaggaaca gcaatcccag ccagagtacc gttaatagat gtttcgcgta
    17821 gatgtgcatg actgtttcat agaaaaaatc atgtttgagc gagaaccgct ggctagcgaa
    17881 atcgaactgc cggtcagcca gtgacaggtc aatagaagtg gcattcgcca tccagacgat
    17941 gccgatggct gcgatcaccc aaagccccca cagagtaatt agtttatctt ttgataacca
    18001 tggtctatgc atgtttttgt ttgttaagta ttgtattggc aagtctatga ggcttaagca
    18061 tgtttgtaca ggattatcat tcactgtctt gtcacaaaca tctgttttaa atcctgacat
    18121 tggatgcaaa gcacatgcca gtttctaata tggaagagat acacttggta agtcactgat
    18181 ttaaattgag aaaagtagat tttatcttgt gctgtggggc gaggtgatag agatgcgcct
    18241 gcaaaaaatg cagatatcag ctggattgct gcgctgatca ctgcaaaagt tgcagtctta
    18301 agctgggtgt gttttttgta acataatgat tttaaatcat aaaattctct ggcatgcggt
    18361 ttgcaaacat ctggatcaag attgttttca gcgagaacat gttatgttgc aggccgaacc
    18421 gaatacaacg ttttaccacg catcatccgc gtatgaattg agaaactttg cccaaaccaa
    18481 acggtatcaa gtggacgaaa tcatcagcca tatggtggag ctggattcgc cttgccgacg
    18541 cgaagtggag tcgtttattg ctgccgtatt taaacaagtg tatggcgcag aagtcagtca
    18601 tttcatgccg caactcgtcg ctttgcgtga tgcgagcggc atcttgatgg ctgcgtttgg
    18661 tttgcacaat gcagcactgg gagacttgtt tcttgagcaa tacctggatg agccgattga
    18721 gcaattactg tcgcgccaat tagaaagaga gattagccgc gcagaaatta cctactttgg
    18781 caatctggcc gtggctaacc ctcgtaatgc cggtgtcctg atcgcgcatg tcatccaaca
    18841 tagcttgaac atgaatttgc cttggggcgt ggcgacggcg catcacagtt tgcagaatgg
    18901 cttgatcaag ggcggtgtgg atttgtttcc attagcgatg gctaatccgg agcgccttag
    18961 cccggaagag cgtgcaaaat ggggcagtta ttacaaacac acgccacaga ttgtggcaat
    19021 ccgtggcgtc gctgaccctg tgtaggtaac tggcaatgat attgcaagaa ttacaggcac
    19081 aagcagaatc tcacccgcac agcgttgcgt tacagggaca agcggtgcag ctcacgtatg
    19141 gtgaattggc cgttgagatc aggcgctggg caagcgcgct gacagggctg gcacctgctg
    19201 ccagtgtcgc tttggcgctc gataatggcc cagcctggat tgtgctggat ctggctatcc
    19261 tggcggcagg ctggaccaat gtacctattc cggcattttt ttctgccacg caaaaacagc
    19321 atgcgattca ggatgcaggt gtgagcctgg tactgacgga tgcgcttgtt gtctggcaag
    19381 gcttataccc gcaaggtgaa gtggtggccg aatggcaaat tgccggtaaa gcggtcacag
    19441 ttttgcggat acatgccagt gatagtgctt ctgctgcgca gaaaatcacc tacacctccg
    19501 gcacgacagg ggctccaaag ggcgtatgcc tgtcgtcaca ggtcatgtgg caagttgcac
    19561 actccattca agcatggaca cagcccagcc aggcagatac acatttttgt gtattgccgc
    19621 tggctacgct gcttgaaaat gtggccggcg tctataccag cctgatgtca ggggcgacgg
    19681 tggtcgtata cccctgccat gaagtcgggt ttagtggtag ccagtttgat attcgccgct
    19741 tgcatcaagg cttgcaacat agtcaggcaa cgactgccat tttgatcccg gaattattaa
    19801 gggcgctggt cgcttatttg caagccgggc atgcgccatt gacggaatta cggtttatcg
    19861 ctgttggcgg tgccaaagtt gcggcggaat tgatacaaca ggcacatttc ttagggttgc
    19921 ccgtttacga agggtatggt ttgtcagagt gcgcttcggt ggtgacgctc aatacaccgc
    19981 agtcacaaaa accaggcagc ataggcaagc ctttgccgca tgtgcagctc cgtgttgact
    20041 acacggggga gctttgggtc aagggcgccc tctatctggg gtatatgcaa gcacagggag
    20101 gtacggtcat gccaacgctg gatgaggacg gctatctggc caccggtgat ctggcatatc
    20161 aagatgatga ggggtattgg tatctttcag gccgcaagaa aaatatgttt attacctctt
    20221 ttgggcgcaa tgtgtcacca gagtgggtag agagtgcctt gttacaccag ccagccattt
    20281 cccaggtgtg cgtatttggt gaagcgaggc catttaatgt ggcgattgcc gtgccgcagc
    20341 cagaggtgag tatagaacaa gtggcccagg caattgccca ggcaaatcaa agcctgcctg
    20401 actatgcaca agtaggcgat gtggtgtttg ccagtcagac atttagtccg gctaacgggc
    20461 aattaacagc caatggccgt ttaaaacgtg acatgattgc tgctgcgtat gctgcggaaa
    20521 tcaatgccat ttacgaagga acgttatgag tttttaccag aaattactga gtgcaaccga
    20581 gcaggaacgt cacacgctga tgtctttgcc actgattacg caaggcgcac aaggcaatat
    20641 cagcctggcg ctttatcgcg ctttcctgac ccaggcgtat catcacgtca agcatacgac
    20701 accattgctg atggcttgtg gtgggcggct aagcggcgaa tatgaatggt tacgtacggc
    20761 gattggtgaa tatattgaag aggaaatggg ccatcaggaa tgggtgctca atgatattga
    20821 ggcctgtggt ggcaataaag aagcggtacg caatagtttg agtgaagagc acgcggcttg
    20881 ccatgctacg gaagtgatgg tggcgtatgc ctacgatacg atttaccggg tgaatccgct
    20941 cggcttcttc ggcatggtat tggtattgga aggtaccagc acggcagttg cgacgcaggc
    21001 tggtgagaaa atcatgcaag ccttaaacct gcctaagaaa gcatttagct acctgttttc
    21061 acatggttcg ctggatatca gtcatgtgtc tttttatgaa agcctgatga accaggtcac
    21121 caaaccggaa gaccagcaag ccatcattca tgccgccaaa cggttttacc gtttatatgg
    21181 tgacatcttt aattacgtgg cgacattgga tcaacacgac aatgtcagga tggggtaaaa
    21241 catggaactc agggatctac atgtggtatt gacgggtgct accggtggtt taggcgcaac
    21301 tttatcactt gcattaagtg ccagaggggc ggtggtttgc ctggttgggc gcgacccggt
    21361 caaattgcat gctttacaga aaagaattgt cgcgcagggc ggaaggtcgc atattgtggt
    21421 ggctgatctg gctcagccca atatggccgg gcaagtgctc aagcaatcat tgcgcgaaat
    21481 ggggcatgta gatttgctga ttaataatgc cgggattatt gaattcaccg aatttgccca
    21541 acaaagcgaa gtcagcattg ataacatggt gcataccaat gtgacggcga cactggtctt
    21601 aagccggacg tttgttgaat actttctaca acgtggccgc gggcacctgg cattcatcgg
    21661 ctccattttt ggctcgttgg gtttcccaca ttttgcgact tactgcgcca gtaaattcgc
    21721 attgcatggg tttagccagg cgttgcgacg cgaactggtg gataccaata tcggcattac
    21781 ttacgttgcc ccccgtgcga ttgcgacccc gatgaatgac gagcgtacca gtgccatgtg
    21841 gcaggcttca gggcaggcgg ttgatcagcc acaagtggtg gcggacaaga tcgtgcgagc
    21901 actggtcaaa gaccagcagg aattgtttat tggtcagcca caaagtttct ttgcctggct
    21961 gaatgggctg gcccccaaat tggtgaacct gggcctcaag caacagactg gcctggcgaa
    22021 aaagttttta taagggatgg ggtgtgttta ctcccactcc agttgcgggt aaatgacatt
    22081 gacattgcgg cggttagcga tgtcgaataa ttgccatttg tctcgctctg aagccgctgc
    22141 ggtatccatg gtttgctcca tgctgatgcc ttgtttaatg ctgctgcgta cgtcagacag
    22201 taacaggttc agataatcca actcattgcc aatgatgctt ttctgatccg tactctccag
    22261 tccatgcccg ggcacaatca gtgtgtagtg cgattgactc aggtgttcca gtgcagaaat
    22321 taggcctttg atatcgcctt caacaacggg tgtacgttgc gcaaataaca agtcgcccgc
    22381 gaacaaggta ttggtgttgg tatccagtac ggttaaatcg gtatgtgtat gtgcaacact
    22441 ttgcgcttgc aattgcagga cgcgtttccc gagatccaag gtctgctggc catgcactgc
    22501 catatctggc gtgaccgtgg tgcccgcttc acccagcagg cgcgcattga gtttttcgta
    22561 ttgctcctgg cgtaataaca tggtgttggc caacttttcg tgaccgacaa aatgcgtttc
    22621 accattttta aacgccgcat tgccataggt atggtcaggg tggacatggg tattgatcac
    22681 atagcgcacc ggcagtgggc tgatggcctt gatggcctgc agcaactgct ctcccgtatg
    22741 tttgctcccg cccgtgtcta tgacggcaat cgcatcccgg ccaatgataa cgcccagatt
    22801 gcaaatatca ccctggtaac catcatcaat atccaggtgt ttgccatggt ggacataaat
    22861 accgtcggca acttgtgtta gctcaaatgt ctctgcgcac aaggggctac aaaacgttac
    22921 aaaagagaat gcaacgctga gacacgagag cttgattttt gataacatga caaccaccca
    22981 ggaatgtttc atatatcata atcaaatcat tcatgacgac aaagtgacta ctgtcacaag
    23041 taacttcaag gagaaaacat gagttccacg caacgctata ccaaagttgc tattattttg
    23101 cactggctga ttgcgctggc cattattgcc atgtttgcgt tgggctggta catgggagag
    23161 ttgcctaaag atggccccaa gcaaacagcg tttgatttgt ttgatctcgg catttatacc
    23221 tggcaaatgg atgaaccagc cagtgccaga accttgtatt tcaatttgca caaatcactt
    23281 ggcttcagcc tgttgtggtt gatcctgttc cgcgtgtact ggcgcatcag ccatactcca
    23341 cctgccatgt tgggcagtta ttccgacctg gagcgcaaag tggcaaccgg cgcccatcat
    23401 ttgttatacc tgctgatggt agcgatgcct gtgagtggtg tactcatgac cttgtacagc
    23461 aaatatggcc tgaaatggtt cgggctggaa gtatttggcg gtttggataa tgcagatttg
    23521 cgtgacctct ttaaagagtc tcatgaactg tttgccaata tactgctggt catttttatt
    23581 gtgcatgtgg ctggtgcgct caaacaccgc tttatcgaca aagatcaaac catgtcacgt
    23641 atgtctttaa aataactaga aacg
//
LOCUS       DATMSK010000075         4891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_428932,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000075 DATMSK010000000
VERSION     DATMSK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428932"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VNZ84_02620"
     CDS             complement(<1..852)
                     /locus_tag="VNZ84_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HWU65874.1"
                     /translation="MRIIKTLFILTLAVSIMSCSVVKTAYNNAPALIAWRLDDYFDFT
                     AAQKAKLKPALQALHAWHRKNELPRYVSLLDEIHDDLSHDMSAQTACQRIEAIKSNVQ
                     TLQTKIIPIIVDVADTLSDKQLQHFEKKLEKRNQEWKEEWWQESIEEQREVRLEKSQD
                     FAEKVYGDLNEKQITLLKQVIARSDIDPAIIYAEILRRDEDTLSILKALRNPASSNEE
                     KSQLVRAGFERMQNSPNAVYQAYIDKLTQQTCETIANLHASTTAKQRLHAQQWVEDYQ
                     TQFTQLQS"
     gene            complement(1012..1539)
                     /locus_tag="VNZ84_02625"
     CDS             complement(1012..1539)
                     /locus_tag="VNZ84_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HWU65875.1"
                     /translation="MNIYQKLMIVAVAVTLPACATNPTGPSNMALPGTGKDFNVFRAD
                     DLSCRDFALTQIGGKTVNQQYNASLAATTAVGTVVGAAIGAAAGGGQGAAIGAGVGGL
                     SGAAVGANTASVSGMNAQDKYDNAYYQCMYAAGHKIPVPASMARTYSQGTNTAPANSG
                     TSYYPPPPPPPGYQH"
     gene            complement(1549..1998)
                     /locus_tag="VNZ84_02630"
     CDS             complement(1549..1998)
                     /locus_tag="VNZ84_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65876.1"
                     /translation="MIKLKKLLFSSLMAGMALASTFAYAHPPGHGGWHGGWRGNVGFY
                     FGSPYPFYPYPYAAYPYPYVYSPPPVIVTQPPQPQVYIEQGSGSTSSTQTAPAQTTPP
                     AASASNGNGQGYWYYCEQSDGYYPYVKECPAGWKQVTPSPPPGTSAK"
     gene            2180..2734
                     /locus_tag="VNZ84_02635"
     CDS             2180..2734
                     /locus_tag="VNZ84_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65877.1"
                     /translation="MQQQSIQQINLSYDPQQDRLLMKTAVSQEQEIQLWITYRVAREI
                     FKVFNREAHLPMSANAPLEHAQTLTDATRQFEQEAEAVQQLDDLDFATAYQQRPSTVS
                     EQVLLVVEARFVSVNDRLNNMQLVCANGINVNMGLSPQLVLAISRMLMIASQEAGWIL
                     PAQKEPEAVSSIVMQVPTEKQVLH"
     gene            2734..3792
                     /locus_tag="VNZ84_02640"
     CDS             2734..3792
                     /locus_tag="VNZ84_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWU65878.1"
                     /translation="MDFTYLGDSGIHVSKVTLGTMTFGDQNTQEDANSQLDFALAQGI
                     NFIDTAEMYPVPPRAETVTRTETMLGHWIKTQRRDRFVLASKVAGPRRGMHWIRGGPT
                     GMDRANIRQAVHDSLTRLQTDYLDVLYLHWPERNVPMFGQYQFDPNAERDANGQPQAW
                     ISIQTQLEVLGELIQEGKIRAIGLSNEQPWGVMEFLRVSREQGLPRIAVLQNCYNLMN
                     RGMEFGMTEVLYRERISLAAYSPLAFGHLTGKYVDDPATPGRVTLFLGYAQRYKKPAV
                     PTASKAYTELARAHGLTPTQLALSFVYHRWFVGTTIIGATSMAQLQENIAAYQVRLSD
                     DVLDAIEQQHLIMMNPAP"
     gene            3819..>4891
                     /locus_tag="VNZ84_02645"
     CDS             3819..>4891
                     /locus_tag="VNZ84_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HWU65879.1"
                     /translation="MLKPGDLRNKILRSFLFFALSLSAGIALGNDLQNALHTCQTEFS
                     NDASRRLVCFDQVAQQYASVPPTAAIHSTKQAALVEKVDAKTNYLARKWHLNADDELH
                     FTDLETYQLNYVVASYSNNPNDVPTSPSRPNTQDRDLNNNDLHFQISLKTQLMNLSDW
                     LPENQWIHSARLWGAYTQQSFWQVYSASQSRPMRDHNYSPELILSLGLNKPGEAKQWA
                     AMPDMLNLGVVHESNGRSEPLSRSWNRIYLQGGWQLNDRYSLLFRPWWRIPESKANDD
                     NPDISKYMGYGDAVLRWDNEANTTAASVTLRNNLRSDNKGYVKLDVQYKPLKSESVRF
                     YAMLASGYGVSLLDYNQAQTMFG"
BASE COUNT         1114 a         1246 c         1343 g         1188 t
ORIGIN      
        1 gctctgcaac tgggtgaatt gcgtctggta atcttctacc cattgttgcg catgcaagcg
       61 ttgtttggcg gttgtactgg catgcaggtt tgcgatggtt tcgcaggttt gctgcgttaa
      121 tttatcgata taagcctggt aaaccgcatt cgggctgttt tgcatacgtt caaagcctgc
      181 gcggaccagt tgcgattttt cttcgtttga cgaggctgga ttgcgtaatg ccttgaggat
      241 agacagcgta tcttcatcgc gccgcaaaat ttcggcatag atgatggctg gatcaatgtc
      301 actcctggca atgacctgtt ttaaaagggt gatttgtttt tcgtttaaat cgccatagac
      361 cttttcagca aaatcctggg atttttccaa cctgacctcc cgctgttctt ctattgattc
      421 ctgccaccac tcctctttcc attcttggtt gcgtttttcc aattttttct cgaaatgctg
      481 cagttgtttg tcacttagtg tatcggcgac gtcgacgatg atggggatga tttttgtttg
      541 aagcgtttgc acatttgatt tgatggcttc aatgcgctgg caggcagttt gtgcgctcat
      601 atcatggctg aggtcatcgt gtatctcatc cagcaacgat acataacgtg gtaattcgtt
      661 tttacgatgc catgcatgca acgcctgcaa tgctggtttc agtttggctt tttgtgctgc
      721 agtaaaatca aaataatcat ccagccgcca tgcgataagc gccggtgcgt tgttgtaagc
      781 ggtcttgacc acgctacaac tcatgattga aacagcgagc gtgaggatga aaagggtttt
      841 gatgattcgc atgtttctac gaccatcact gacaagctaa gtttgcgata ccgtcaattg
      901 gcaatcactg cttagagtgt ctatgcggtc agatgccctt gcctgacctt ggcactgaat
      961 gatgaagaga ctgggtaatg ccaggcagcg catggctgcc tggtggccgg gctaatgttg
     1021 atagccagga ggtggcggag gtggggggta atagctggtg ccactattcg ctggtgcggt
     1081 gtttgtcccc tgcgaatagg tccttgccat gctggccggg accgggattt tatgcccggc
     1141 cgcatacatg cactgatagt aggcattgtc gtatttgtcc tgcgcgttca tgccgctgac
     1201 agacgccgta ttggcgccca cagcggcacc cgacaatcca cctacgccag caccgatggc
     1261 ggcgccttga ccaccgcctg cggctgcgcc aatcgctgca ccgacgactg tccctaccgc
     1321 cgtggtggct gccaggctgg cgttatattg ctggttgacg gttttgccgc caatttgtgt
     1381 cagtgcaaag tcacggcagg ataagtcatc ggcacggaac acattaaagt ccttacctgt
     1441 gcctggcaac gccatattgc tgggcccggt cgggttggtg gcacaggcag gcaaagtcac
     1501 tgccactgcc acaatcatca atttctggta aatgttcatg gctttacctt acttggcaga
     1561 ggtgccaggt gggggtgaag gcgtcacctg tttccagcca gccgggcatt ctttcacata
     1621 gggataatag ccgtcagact gttcgcagta ataccaatag ccttgcccat tgccgttgct
     1681 ggcactggct gcaggcggtg tcgtctgtgc gggtgccgtt tgtgtggatg atgtgctacc
     1741 gctaccttgt tcgatataga cttgtggctg tggtggttga gtcacgatca caggtggtgg
     1801 gctatagaca taagggtagg gataagccgc atagggatac ggataaaatg ggtagggtga
     1861 gccgaagtaa aagcccacat tgccgcgcca gccaccatgc catccgccgt gtccaggtgg
     1921 gtgcgcatag gcgaaagtgc tggcaagtgc catgcctgcc atcaggctgc taaacaataa
     1981 ttttttcaac ttgatcatat cgcctccgtc acgaatactc ctgtttgtaa gacagaagca
     2041 tacgcatatt tatccggcaa gaccggggcg gttttgtatc agagtgtatc tttgcggggc
     2101 ctggtggcgg atagtgttag gtagccaccg cctaactgcg gtatgcttat ccatgaacgt
     2161 ttgcaaatcg tgaatcctca tgcaacagca atccatacag cagatcaatc tttcttacga
     2221 cccgcagcaa gaccgcttgc tgatgaaaac ggctgtcagc caggagcagg aaatccagtt
     2281 gtggatcacc taccgggtgg cccgagaaat cttcaaggtc ttcaaccgcg aggcacattt
     2341 accaatgtcg gcaaatgcgc cacttgagca tgcgcagacg ctcacggatg cgacacgcca
     2401 gtttgaacag gaggccgaag ccgtgcagca actggatgat ctggattttg ccaccgccta
     2461 ccagcaacgg cccagcacgg tcagcgagca ggtgctgttg gtggttgaag cacgttttgt
     2521 ttccgtgaat gaccgcttga acaatatgca attggtctgc gccaatggca ttaacgtgaa
     2581 tatgggcctg agcccgcaat tggtgctggc gatcagccgt atgctcatga ttgccagcca
     2641 ggaagcaggc tggatactgc ctgcacaaaa ggaaccagaa gcggtttcca gcatcgtaat
     2701 gcaggtacct acagaaaaac aggtgttaca ttgattgatt ttacttattt gggtgacagc
     2761 ggcattcacg tgtcaaaagt gacgctgggc accatgacct ttggtgacca gaatacccag
     2821 gaagacgcga acagccagct cgattttgcc ttagcgcaag gcattaactt tattgatacg
     2881 gccgagatgt atccggtgcc gccgcgggca gaaaccgtga cacggaccga aaccatgttg
     2941 gggcactgga tcaagacaca gcggcgcgac cgctttgtgc tggccagcaa agtggccggc
     3001 cccaggcgtg gtatgcactg gatcaggggt ggcccgaccg gcatggaccg cgccaatatc
     3061 cgccaggctg tgcatgacag cctgacacgc ttgcaaaccg attatcttga tgtgctttac
     3121 ctgcactggc ccgaacgcaa tgtgcccatg tttggccaat accagttcga ccccaatgcc
     3181 gagcgggatg cgaatggcca gccccaggca tggatatcta tccagaccca gcttgaagtg
     3241 ctgggcgagt tgatccagga aggcaaaatc cgggcgatag gcttgagtaa tgagcaaccc
     3301 tggggcgtga tggagttttt gcgcgtgtca cgcgagcagg gcttgccgcg gattgcggtg
     3361 ctgcaaaatt gctacaacct gatgaatcgc ggcatggagt ttggcatgac cgaggtgctt
     3421 taccgcgagc gcatcagcct ggccgcctat tcaccgctgg cgtttggcca cctgaccggc
     3481 aagtatgtgg acgaccctgc gacgcctggt cgcgtcactt tattcctggg atatgcccag
     3541 cggtataaaa agccggcagt gcccacagcc tctaaagcct ataccgagct ggcccgggcc
     3601 catgggttga cgccgacgca attggcgctc agcttcgtgt atcaccgctg gttcgtgggg
     3661 accacgatta tcggtgccac cagcatggca caactgcagg agaacattgc ggcgtatcag
     3721 gtccgcctga gcgatgacgt attagatgcc attgaacaac aacacttgat catgatgaat
     3781 ccggcgccct gaccattgcg ccaaagaagg gaatccacat gttaaaacct ggcgacctgc
     3841 gcaacaaaat actgcgcagc ttcctttttt ttgccttgag cctgagcgca ggcatagcct
     3901 tgggaaatga tttgcagaat gccctgcata cctgtcaaac cgagtttagt aatgatgcca
     3961 gccgccgttt ggtatgcttc gaccaggtcg cgcagcaata tgcttcggtg ccgcctacgg
     4021 cggctattca ctcaaccaag caggctgcgc tggtggaaaa agtggatgca aaaaccaatt
     4081 acctggcgcg taaatggcac ttgaatgccg atgatgaact gcactttacc gacctggaga
     4141 cctaccagct caattacgtg gtggcgtctt attccaataa cccgaatgat gtgccgactt
     4201 caccttccag gcccaatacc caggatcgcg atttaaataa taacgacctg cacttccaga
     4261 tcagcctgaa aacccagctc atgaacctgt ctgactggtt gcctgaaaac cagtggatac
     4321 atagtgcgcg tttatggggt gcttatacac agcaatcttt ctggcaggtg tatagtgcca
     4381 gccagtcacg tcctatgcgg gatcataatt actcgccgga attaatcctg tcattaggtt
     4441 taaacaagcc tggggaagcc aagcaatggg cggccatgcc agatatgctt aacctgggcg
     4501 tcgtgcatga atccaatggg cgttccgagc cgctttcacg cagctggaac cggatctacc
     4561 tgcaaggggg ctggcaactg aatgaccgct acagcctgtt gttccgtccc tggtggcgta
     4621 ttccagagag caaggccaat gacgataacc ctgatatcag caaatacatg ggctatggcg
     4681 atgcggtctt gcgctgggat aacgaggcaa acaccacagc cgccagcgtg actttacgta
     4741 ataacttgcg cagtgataac aagggatatg tgaaactgga tgtgcagtac aaaccgctca
     4801 aaagtgaaag tgtgcgcttt tatgccatgc tggcgagcgg ctatggcgtt tcgttactgg
     4861 attacaacca ggcacaaacc atgtttggca t
//
LOCUS       DATMSK010000076         5905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_29943,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000076 DATMSK010000000
VERSION     DATMSK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29943"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="tpiA"
                     /locus_tag="VNZ84_02650"
     CDS             complement(<1..777)
                     /gene="tpiA"
                     /locus_tag="VNZ84_02650"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWU65880.1"
                     /translation="MSRSDISHVSSHKLVVANWKMHGNLARNQTLIHGYLQGLQALPQ
                     TDVIVCVPYPYLGQMQSLLTGSRLAWGAQNIAKYEEGAYTGEVSAEMLRDFAAQYVII
                     GHSERSTAYCESDENIAEKFMMAKRHGLIPILCVGETLLEREAGVMERVVGKQLETII
                     RLFGGEAFAGTIVSYEPIWAIGTGLAASAEQAVAMHTFIRETVAAADKAAAQTLKILY
                     GGSVNPQNAVQLLNQQEIDGALVGRCSLEVEQFIKICQAVP"
     gene            complement(927..2006)
                     /locus_tag="VNZ84_02655"
     CDS             complement(927..2006)
                     /locus_tag="VNZ84_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HWU65881.1"
                     /translation="MALDLFSPVKLGSLALQNRVVMAPLTRNRAGEGGVPQAMNVTYY
                     EQRATAGLIITEATPISPMAHGYPALPGIYTDAQVAGWKKVTDAVHAKGGKIVIQLWH
                     VGRISHPSLLPNQATPVAPSAIKPAGQAFTYQGLQDFVTPRALEVTELAGIVQDYVHA
                     TRCAIAAGFDGVEVHAANGYLLDQFLRDGTNQRQDQYGGSIENRSRLLREVVQAVVDT
                     IGADKVGVRLSPVNPFNDMHDSQAQNTFDAVTKTLNAFNLAYLHVVEGGIHGGGESEP
                     FDFAAMRKLFNHGYIANLSYDKARGNAAIASGHADAIAYGVPFIANPDLVARLAKDAP
                     LNQADQSTFYGGTEHGYTDYPFLMA"
     gene            complement(2105..2677)
                     /locus_tag="VNZ84_02660"
     CDS             complement(2105..2677)
                     /locus_tag="VNZ84_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWU65882.1"
                     /translation="MKKIILALGLLAATQAWAATDTYNIDATHSFANFSIRHVVSKTS
                     GTFNDVTGVITLDPANLNTATVRAAINVSSVNTGFGKRDEHIKADKYLDVGKYTQILF
                     ESTKITASNQTQAVMQGKLTMHGVTKLVDIPVKVLGYGADPWGGQRAGFEGHLTIKAS
                     DYGFGWATGAGAPVGDDIEITLLIEGVKAK"
     gene            complement(2715..3287)
                     /locus_tag="VNZ84_02665"
     CDS             complement(2715..3287)
                     /locus_tag="VNZ84_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HWU65883.1"
                     /translation="MTQVSIVYHSGYGHTAEQAKAVARGAQKVAGVTVNLLTVDEAQQ
                     QWEVLKASDAIIFGSPTYMGSASGQFKTFMDASSKAWYTRDWKDKVAAGFTTSASQSG
                     DKLSTLIQLAVFAAQHGMIWVGQDLLPGNNNSQGSVNDLNRLGSFLGAMAQANSDQGP
                     DLAPIQSDLLTAEHLGERVATITQRFSKSA"
     gene            complement(3319..4029)
                     /locus_tag="VNZ84_02670"
     CDS             complement(3319..4029)
                     /locus_tag="VNZ84_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWU65884.1"
                     /translation="MLHVRPSNTRGYAHHGWLESFHSFSFASYYDPDNIGYSVLRVIN
                     DDKIAPAAGFGMHGHQDMEIITYMLEGELRHTDSMGHSEVIRAGDVQRMTAGTGVRHS
                     EMNASGQTTAHLLQIWITPDTLGLAPGYEDKTISVADKQNRWALIASPGAQAGSLKVH
                     QDVSLYATVLSADAALPVHVMAGRSAYLHIARGEVEIEGQRLQSGDALKVDAASELNM
                     RAISNSEVLWFDLPQVTE"
     gene            4134..5051
                     /locus_tag="VNZ84_02675"
     CDS             4134..5051
                     /locus_tag="VNZ84_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU65885.1"
                     /translation="MTIKLSLEALEVIDAIDRKGSFAAAAESLYRVPSALTYTIRRLE
                     EDLGLELFDRSGHRATLTAAGAELLKEGRYLLDAAHALEQRVQRIATGVETDITLAIS
                     DLFDFHPILQILQDFYQQGFGTRVKLLREVYGGSWDALQSGRAELSIGAPGDAPPGGV
                     FSTHLLGQLEFVFAVAPHHPLAALPEPLSASDIVQHRVVAAADSSRNLPPRTSGILTG
                     QDILTVPDMHTKLQAQISGIAIGYLPKFLAQSAANQGQLIIKQVSEPKPLAPIFIAWR
                     QQRPAEMGKAMQWLLKQCAQLSLEQLLNK"
     gene            5088..5543
                     /locus_tag="VNZ84_02680"
     CDS             5088..5543
                     /locus_tag="VNZ84_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HWU65886.1"
                     /translation="MSQEQLPEEFEPLNRIAIKAMLWLNGLAHIIIGLALAVSVIMFT
                     WLFFLDVKEAAYAHNLVHGFLRALGTLMLLWSISALISAEIRYLRGHKLLVETFVEVV
                     LVMFLRKLIVLPVQDATPTYEEIGIWLAGAFVMGLVYILIRLADKNSSR"
     gene            5546..>5905
                     /gene="tadA"
                     /locus_tag="VNZ84_02685"
     CDS             5546..>5905
                     /gene="tadA"
                     /locus_tag="VNZ84_02685"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HWU65887.1"
                     /translation="MQDEDFMRMAIALAQEAASLGEVPVGAVVVKNGVVIGQGRNAPI
                     SLNDPTAHAEIQAMREAAQYLNNYRLVGCTLYVTLEPCAMCSGAIQHARIARLVYGAS
                     DAKTGCCGSVIDLMKEQK"
BASE COUNT         1484 a         1660 c         1333 g         1428 t
ORIGIN      
        1 agggacggcc tgacagattt ttataaattg ctcaacttct agcgaacaac ggccgaccag
       61 cgcaccatct atttcctgct gatttaataa ttgtaccgca ttttgcgggt ttacgctgcc
      121 cccatagagg atttttagcg tttgagccgc agctttatct gcagccgcaa cagtctcacg
      181 aataaacgtg tgcatggcca ccgcctgctc ggcagaagcc gccagtccgg taccaattgc
      241 ccagattggc tcataggaga caatagtccc tgcaaacgcc tccccgccaa acaggcgaat
      301 aatggtttca agctgtttgc caaccacgcg ctccatcaca cccgcctcgc gttccagcaa
      361 ggtttcaccg acacataaaa tcgggatcaa accatgacgt ttggccatca taaatttttc
      421 ggcaatattt tcatcagact cacaataagc cgtgctgcgc tcagagtggc cgataatgac
      481 atactgcgcc gcaaagtctc gcaacatttc cgcactcacc tcaccggtat aagccccctc
      541 ttcatatttg gcaatatttt gcgcccccca tgccaaacgg ctgccagtaa gcaaggattg
      601 catttgcccc aggtaagggt aaggtacaca cacaatcaca tcggtttgcg gcaacgcttg
      661 cagcccttgc aggtatccat ggatcaaagt ttggttacgg gcaagattgc catgcatttt
      721 ccagttggca acaactagtt tatgagatga aacgtgggag atatcagaac gtgacatgca
      781 gaaattaact cacaatgtgc gaagcaaagg cttcgaccga attcgcgttg cgcgaatttt
      841 acgctggcac actgtgagca gcaagtataa agacaaatat tgccttgggc aggcaacccg
      901 cttacccgcc caacacggaa agtgcactaa gccatcagga atggataatc tgtataccca
      961 tgctcggtgc cgccataaaa cgtggattga tccgcctgat ttaacggcgc atccttggcc
     1021 agacgcgcca cgagatccgg attggcaata aatggtacgc cataggcaat cgcatccgca
     1081 tgtccactgg caatcgccgc attgccgcgc gccttgtcat aactcaggtt agcaatatag
     1141 ccatgattaa atagtttgcg cattgccgcg aaatcaaacg gctcagactc accaccaccg
     1201 tgaatacccc cttcaaccac atgcaaatag gccagattga atgcattcaa agtcttggtg
     1261 accgcatcaa acgtgttctg cgcctgacta tcatgcatat cattgaatgg attcaccggt
     1321 gataatcgca cacctacttt atccgccccg atggtatcca caaccgcttg cactacctca
     1381 cgcaacaagc ggctgcggtt ttcgatcgag cctccatatt gatcctggcg ctgattagtg
     1441 ccatccctca ggaactgatc cagcaggtaa ccattggcag catgcacttc tacaccatca
     1501 aaaccggcag ctatggcgca tcgtgtcgcg tgcacataat cctgcacaat acccgccaac
     1561 tcagttacct ccagcgcgcg gggtgtgaca aaatcctgca agccttgata agtaaaggcc
     1621 tgtcccgccg gtttaatggc tgatggtgcc acaggcgtcg cctggttagg cagcaaactc
     1681 gggtgagaga tgcggccaac atgccataac tgaatgacaa tttttccacc cttagcatgt
     1741 acggcatcgg tgactttttt ccagccagcc acttgtgcat cagtatagat cccaggcaaa
     1801 gctggatagc catgcgccat gggcgaaatc ggcgtcgctt ctgtaataat caacccagcc
     1861 gtggcgcgct gctcgtagta agtcacattc atcgcctgcg gcacaccgcc ctcaccggca
     1921 cggttacgcg tcaaaggtgc catcacaaca cggttctgca gtgcaagact tcccagtttt
     1981 accggggaaa acaaatccaa tgccatcttt atctccatct attcaattgc gcctgaaaaa
     2041 caactatgcg gacttgagcc tacataccca agtccgcaca tcatgcctac aaaaacttta
     2101 agatttactt ggctttaacg ccttcgatca gcaaggtgat ttcaatatca tctccgactg
     2161 gcgctccggc accggttgcc cagccaaagc cataatcaga agccttgatc gtcaagtgac
     2221 cttcaaagcc agcgcgttgg ccgccccatg gatctgcacc atacccaagc actttgaccg
     2281 gaatatccac cagcttggtg acaccatgca tggtcaactt gccttgcatc acagcctgtg
     2341 tctggttgct tgcagtaatt ttggtgcttt caaacagaat ttgcgtgtac ttgcctacat
     2401 cgaggtactt gtccgcttta atatgctcat cacgtttacc aaaaccggta tttacactgc
     2461 tgacattgat cgcagcacgc actgtggccg tgttgaggtt agcaggatca agcgtaatca
     2521 cgcctgtcac atcgttaaaa gttccagagg ttttggatac cacatgacgg atactgaaat
     2581 tggcaaaact atgtgtcgca tcaatattgt aggtatcggt tgcagcccaa gcctgcgtcg
     2641 ctgctaacaa acccagcgca agaatgattt ttttcactta ttgtctcctt agtgtttggt
     2701 cttttaccgc tgcattaagc ggatttggaa aatcgctggg taatcgtggc cacacgttca
     2761 cccaagtgct cagcggtgag taaatcactc tgaatgggtg ccaagtcagg cccctgatcg
     2821 gagttagcct gcgccatggc acccaaaaag ctgcccaaac gatttaaatc gttgaccgag
     2881 ccttggctat tattgttccc tggcaataaa tcttgcccca cccaaatcat gccatgttgt
     2941 gccgcaaata ccgccaactg gatcagagta ctcaatttat ccccgctctg gctggcagat
     3001 gtggtaaacc cggcggcgac tttatctttc caatcacggg tgtaccatgc ttttgagctg
     3061 gcatccataa aggtcttaaa ctgaccggaa gcagaaccca tataggtggg actaccaaaa
     3121 ataatggcat cactggcctt gagcacctcc cattgctgct gcgcttcgtc cacagtcagc
     3181 aaattcacgg tcacaccggc cactttttgc gcaccacgcg ctaccgcttt ggcctgctcg
     3241 gcagtgtgtc cataaccact atgataaaca attgaaacct gagtcatttt ttagctcctt
     3301 atcaacaatt caaaaacatt attcggtcac ttgtggcaaa tcaaaccaca acacttcact
     3361 attactgatt gcgcgcatat ttaactcact ggctgcatcc accttgagcg catcaccact
     3421 ttgcagtcgc tgcccctcaa tttctacttc accgcgcgca atatgcagat atgcggaacg
     3481 gccagccatc acatgcacag gcaacgctgc atccgcactc agcaccgtcg catacaatga
     3541 cacatcctga tgtactttta atgaccctgc ctgcgcacct ggtgaggcaa tcagtgccca
     3601 gcgattttgc ttgtctgcca cggaaatggt tttgtcctca taaccaggcg ccagccccaa
     3661 agtgtctggg gtaatccaga tttgcaataa atgtgcggtg gtttgcccgg atgcattcat
     3721 ctcgctatgt cgcacaccag tacctgccgt catacgctgt acatcgccag cacgaatcac
     3781 ttcgctatgc cccatactgt ctgtatggcg caactcacct tccagcatgt aggtaataat
     3841 ttccatatct tggtgaccgt gcataccaaa gcctgccgcg ggagcgatct tgtcgtcatt
     3901 gatcacccgc aataccgaat accctatatt gtcaggatcg taataactgg caaatgaaaa
     3961 actatggaag ctctccagcc agccatgatg tgcgtaacca cgtgtatttg aagggcgaac
     4021 atgtaacatt tattcactcc gtttgaaaga ttaaatgtat gataaaaaaa cacttaatct
     4081 tttgatagca aaacattttg catttcttgt tcagtttttt tgattaatat ctcatgacca
     4141 ttaaactcag cctggaagct ctggaagtca ttgacgccat cgaccgcaaa ggcagttttg
     4201 ctgccgcggc agaatccctg taccgcgtcc cctccgcatt gacttacacc atacgtcgcc
     4261 tggaagaaga cttggggctg gaattatttg accgcagcgg ccaccgtgcc accttgactg
     4321 ccgctggtgc cgaactactg aaagaagggc gttatttgct ggatgccgcc catgcccttg
     4381 aacagcgggt gcaacgcatt gccaccgggg tagaaaccga tattacgctc gccatcagcg
     4441 acctgttcga cttccatccg atcttgcaga ttttgcaaga cttttaccag caaggctttg
     4501 gtacccgggt aaaactgttg cgcgaagtat atggcggcag ctgggacgca ctacaatccg
     4561 ggcgagcaga actctcaatc ggcgcacccg gtgatgcgcc acccggcggt gtattctcca
     4621 cgcacctgct cggccaactg gagtttgtat ttgcagttgc cccccatcac ccactagccg
     4681 ctctgccaga gcctttaagt gccagtgata ttgtgcagca ccgcgtcgtc gcagcggcag
     4741 acagctcacg caacctgcca ccacgcactt ccggcatcct gacgggacaa gacatactga
     4801 cggtaccaga tatgcatacc aagttgcagg cgcaaatatc tggcattgcc atcggctacc
     4861 tgccaaaatt cctggcacaa tccgctgcca atcaagggca actcatcatc aaacaagtct
     4921 ccgagcccaa gccacttgcg cccatcttca ttgcctggcg ccaacaacgc ccggctgaaa
     4981 tgggcaaagc catgcaatgg ttgctaaaac aatgcgccca actgtcattg gagcaattgc
     5041 tcaacaaatg atttatcatc atcgatgtct gatgaaatgg atttaagatg tctcaagaac
     5101 aactgcctga agagtttgaa ccgctcaacc gcatcgcgat taaagccatg ctctggctca
     5161 atggtctcgc ccacatcatc attggcctgg cgttggccgt ctccgtcatt atgtttacct
     5221 ggctgttttt tcttgacgtg aaagaagccg cttacgcaca caaccttgta cacggtttct
     5281 tgcgagcact tggcacactg atgttgctgt ggtcaatttc ggccctgatt tctgccgaaa
     5341 tccgctacct gcgtggtcat aaactgctgg ttgaaacctt tgtcgaggtt gtactggtga
     5401 tgtttttgcg caaactgatc gtattacccg tgcaagatgc tacgcctaca tatgaagaaa
     5461 taggcatctg gctagcaggg gctttcgtca tggggctggt ctatatcctg atccgccttg
     5521 cagataaaaa cagtagcaga taaccatgca agacgaagac tttatgcgca tggcgatcgc
     5581 actggcccag gaagcggcaa gcttgggtga agtgcctgtg ggtgcagtgg tggtaaaaaa
     5641 tggtgtggtc atcggccaag gccgcaacgc tcctattagc ctcaacgacc ccactgcgca
     5701 cgctgaaatc caggccatgc gcgaagccgc gcaatatctg aacaattacc gcctggtcgg
     5761 ctgcacactt tacgtcaccc tagagccctg tgcgatgtgt agcggcgcta tccagcatgc
     5821 ccgtatcgcc cgactggttt atggcgcaag tgatgctaaa accggttgct gtggcagcgt
     5881 gattgacttg atgaaagaac aaaaa
//
LOCUS       DATMSK010000077         7092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_241519,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000077 DATMSK010000000
VERSION     DATMSK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241519"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..818)
                     /locus_tag="VNZ84_02690"
     CDS             complement(36..818)
                     /locus_tag="VNZ84_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HWU65888.1"
                     /translation="MMAAFQVYQAAFTAHLRHPSKNSTPADVSEQRMRVYREIVFNNF
                     FASVSVCFPVLLGILGKRRFTQLVRQCFFSQPFKSPLFQDIPGSFVDFLQSLVLPETG
                     LPDYTAQLAHYEWIELALSRQVDSEPVAVLTEPMLEASALLYCEVQLPAAHRLLRYDF
                     PVHQLSKKQSTIAPAATFLLVYRTADFQIRFIQLNAATYQLLQQLQAHRKTTHHHLQD
                     LAVSMPQLPRESVIQFGLETLYALYEQQTIRVSSHQNMSSDP"
     gene            complement(815..1693)
                     /locus_tag="VNZ84_02695"
     CDS             complement(815..1693)
                     /locus_tag="VNZ84_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HWU65889.1"
                     /translation="MAHLSDIHGAGLGLKRELLPQLIQQQGQPWLSNLQFIEIAPENW
                     IAAGGKYAVELDWLRRHYPIVCHGLCLSLGGPDPLNLSFLKQVKQFLADFEIPLYTEH
                     LSYCSDTANGYLYDLLPIPFTEEAVRYVANRIRQVQDILQQRIAVENTSFYVAAPISE
                     MDEISFINAVLSEADCDLHLDLNNVYVNSVNFGFDPHLFLDQIPAERVVYSHMAGHLQ
                     VEPGLLVDTHGEAIIDPVWHLLEYAYKSFGAFPTLLERDSNIPPLATLMLEVDRIAHL
                     QNHARSKHQPSRDKVA"
     gene            complement(1712..2023)
                     /locus_tag="VNZ84_02700"
     CDS             complement(1712..2023)
                     /locus_tag="VNZ84_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65890.1"
                     /translation="MTTHKIAVITGAVALSIAASSANAENPFELKTLAQGYQVADASD
                     SKAKEGKCSSGKCGADKKHVKEDGAATGKTKEGSCHHHKSKEGSCSAEKMKEGSCHAS
                     K"
     gene            complement(2061..2309)
                     /locus_tag="VNZ84_02705"
     CDS             complement(2061..2309)
                     /locus_tag="VNZ84_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWU65891.1"
                     /translation="MLSCKQTSLLVSQSLDRPLTWRERWAVRVHLLICVYCRRFTQQL
                     KLIRRYMERWQQQVAEGEDIQLSLAARERITQQLDKFY"
     gene            complement(2337..2879)
                     /locus_tag="VNZ84_02710"
     CDS             complement(2337..2879)
                     /locus_tag="VNZ84_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWU65892.1"
                     /translation="MATNPHDWLNEHGDYLYRFALARLRDPHQAEDVVQETLLAAIKN
                     PNFAEQSSPRTWLTGILKHKIIDCMRKQVRETAVSELLSEEDANMDDFFDDTGHWAEK
                     PQVWDIPHDALQQTQFLKVLQQCMERLPQKLAAIFTMRDVDEMDNEEICKELEITTTN
                     AWVMLYRARMGLRKCLEIHW"
     gene            complement(2900..3349)
                     /locus_tag="VNZ84_02715"
     CDS             complement(2900..3349)
                     /locus_tag="VNZ84_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HWU65893.1"
                     /translation="MALFNPSRDEVRQFFFDAWAKFKQQHMLTELEKMAVGIMHMHPE
                     YHAILDHPEQYLEQTYYPEMGETNPFLHMSLHLSIQEQISINQPIGITPAYGKLCTRF
                     QEEHEAQHALLECLAETIWLAQRNQTGLDAAHYLNLIEQRAGIAPSA"
     gene            complement(3352..4011)
                     /gene="nth"
                     /locus_tag="VNZ84_02720"
     CDS             complement(3352..4011)
                     /gene="nth"
                     /locus_tag="VNZ84_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWU65894.1"
                     /translation="MNAQKRLAIFERLAQAIPNPTTELEHSSTFELLIAVILSAQATD
                     KGVNIATRKLFKVANTPQAILDLGLEGLESHIKTIGLYHAKANNVLKCCHQLITLHGG
                     EVPDNREALEALAGVGRKTANVILNTAFGQPTIAVDTHLFRVGNRTKLAPGKNVMEVE
                     KRFLSTTPKQFLKDAHHLLILHGRYTCIARNPKCGECCIADLCEFHDKQIYPVAPPAG
                     A"
     gene            complement(4041..4715)
                     /locus_tag="VNZ84_02725"
     CDS             complement(4041..4715)
                     /locus_tag="VNZ84_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HWU65895.1"
                     /translation="MQTPRHDQTSGQDTSGLEALVVQIDALLPQTQCRQCGYDGCKPY
                     AWAIARKEANINQCPPGGEAGIHALAQLLQRPYQALNPVHGVTKPKAVAWIDEETCIG
                     CTLCIQACPVDAILGASKLMHTVITQECTGCELCLPPCPVDCIDMQPIPGRPVAMSKT
                     EADQARRRHQQRLARLALEKQAKAHTYNKHKLTPEIPEVQANTDSLKKTALAAAMARV
                     KAQKTG"
     gene            4951..5829
                     /gene="dapA"
                     /locus_tag="VNZ84_02730"
     CDS             4951..5829
                     /gene="dapA"
                     /locus_tag="VNZ84_02730"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWU65896.1"
                     /translation="MLTGSLVAIVTPMFEDGRLDLDALKKLIDFHVDAGTDGIVIVGT
                     TGESPTVDVDEHCLLIKTTIEHVAKRVPVIAGTGANSTAEAIELTAKAKSLGADACLL
                     VAPYYNKPSQEGLYQHFKAVAEAVDIPQILYNVPGRTGCDLSNETVLRLAKIPNIVGI
                     KDATGGIERGTDLLLRVPADFAVYSGDDATSLALMLLGGKGVISVTANVAPKLMHEMC
                     EQALKGNLAAAKAANAKLFALHQKLFVEANPIPVKWVLQQMGLIGTGIRLPLVNLSSQ
                     YHEVLRNAMQQAEIPA"
     gene            5869..>7092
                     /gene="bamC"
                     /locus_tag="VNZ84_02735"
     CDS             5869..>7092
                     /gene="bamC"
                     /locus_tag="VNZ84_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HWU65897.1"
                     /translation="MSHVWLQRVVLASLVTVLAACDSIPFIDNSSDYKGAGRSKPLEV
                     PPDLTAVRTSSTYNVPGSTSYSAYSQGQEAQEQNGPQPVLADMKNVRMVKAGQQRWLV
                     VNAPPEKIWPIVRDFWLDQGFAVRVENPELGVIETEWLQSDAIKPKEDNRGYGEKFDA
                     WLDKLSGFADRRKFRTRLERGEKDGTTEIYMTHRTVAGAPDDGKNYVQTQLGVIDTGY
                     RKDAAENKNNAGQEFDADLDAELLRRMMVKLGLDEQKADQVMTQAASDKRADVVKESD
                     QSVTLKLNEPFDRAWRRVGLALDRIGFVTEDKNRSEGIFYVRYADTDAEDPIKQKKGL
                     LERLKFWGSDDDDKSTTPKDVKPGDKTKFWKGTSDGDKSSKQYHIQVLENADDTTDVY
                     VLTPENKRNTSTTANR"
BASE COUNT         1703 a         1645 c         1898 g         1846 t
ORIGIN      
        1 ccacacttcc tggtctccaa gacaggctgg tagtgtcatg gatcagatga catattctgg
       61 tggctactca cgcggatggt ttgttgttcg taaagtgcgt atagcgtttc caggccaaac
      121 tggatcaccg attctcgggg taattgcggc atgctcacgg ctaaatcttg caggtgatgg
      181 tgtgttgttt ttctatgtgc ctgcaattgt tgcaatagct gataggtcgc tgcattgagt
      241 tgtatgaatc tgatctggaa atctgctgtg cgatagacca ataaaaaggt ggctgcaggc
      301 gcgatcgtgc tttgcttttt agagagctgg tgcaccggaa agtcataacg tagcaggcga
      361 tgtgctgctg gcaattgcac ctcacaatat aacagtgcgc tggcctccaa catcggctca
      421 gttaaaacgg ccactggctc agaatccact tgcctgctga gcgccagttc tatccattca
      481 taatgggcaa gttgtgctgt gtagtctggt agtccggttt ccggtaatac taatgattgc
      541 aagaaatcaa caaaactgcc tggaatatct tggaataacg ggcttttaaa tggctgtgaa
      601 aagaaacact gccggaccag ttgagtgaaa cggcgtttgc caagaatgcc cagcaacaca
      661 ggaaagcaca cgctgacgct ggcgaaaaaa ttgttaaaga caatttcacg gtaaacgcgc
      721 atgcgttgtt cactgacatc cgctggcgtg ctgtttttgg aagggtgccg caaatgtgcg
      781 gtgaacgctg cctggtaaac ctggaaggca gccatcatgc aaccttgtct cgggagggct
      841 ggtgcttaga gcgagcatga ttttgcaagt gggcgatacg gtccacttct agcatcaagg
      901 tcgctaaggg agggatattg ctatcgcgtt ccagcaaagt gggaaacgcg ccaaagcttt
      961 tatacgcata ctcaagtaag tgccataccg ggtcgataat cgcctcgcca tgagtgtcaa
     1021 ccagtaggcc aggttccact tgcagatgcc cagccatatg gctgtagact acgcgttcag
     1081 ctggaatctg atccaggaat aggtgaggat caaagccaaa attgacacta ttcacataca
     1141 cattgttgag gtcaaggtgt aagtcgcagt cagcctcaga cagcactgcg ttaatgaagc
     1201 tgatttcatc catctcggaa atcggtgcag cgacataaaa ggaagtgttt tctacggcta
     1261 tgcgttgctg cagaatatct tgtacctggc ggattcgatt ggccacatag cggacagcct
     1321 cttcagtaaa cggaatcggt agcaagtcgt agagataacc gttggccgtg tcactgcaat
     1381 aacttaaatg ttcggtatag agggggatct caaaatcagc caggaattgc tttacctgtt
     1441 tcaggaacga gaggttgagc ggatcaggcc cgcccagtga caggcataac ccatggcaaa
     1501 caatcggata atgccggcgt aaccagtcga gctctactgc atatttcccc cctgccgcga
     1561 tccagttttc aggggcgatt tcgataaact gtaaattcga tagccagggt tgcccctgtt
     1621 gttgaatgag ctggggcagt agctcgcgtt taagccctag cccagcgcca tgaatatccg
     1681 acagatgtgc catgcgccgc aggtgaggtg attatttgct ggcgtggcag gatccttctt
     1741 tcattttctc tgccgagcat gacccctctt tagatttatg atgatggcaa gatccttctt
     1801 ttgttttgcc ggtggcagcc ccatcctctt ttacatgttt cttgtctgcg ccacatttac
     1861 cactgctgca tttaccttct ttggctttgc tgtcagaggc atctgcaacc tggtagcctt
     1921 gtgccagtgt ttttaattca aacggatttt ccgcgttggc agatgatgca gcgatggata
     1981 aagccacggc accagtaatg acagcgatct tatgagtagt catgtgaatt ccttttcaat
     2041 acagagttta aaagcaggcg tcagtagaac ttatccaatt gctgcgtaat acgttccctc
     2101 gctgccaacg ataactgaat atcctcgcct tcggcaactt gttgctgcca acgttccata
     2161 tagcggcgaa tcaacttcag ctgttgcgta aagcgtcggc aatacacaca aatcagcaaa
     2221 tgaactctga cggcccaacg ctcacgccaa gtgagggggc ggtcaagtga ttgcgacacc
     2281 agcaagctgg tttgtttgca actcaacatc tctttttccg tttcacaatt gacgtgttac
     2341 caatgtattt caaggcattt ccgcagtccc atgcgggcac gatataacat gacccaggca
     2401 ttggtcgtgg tgatctcgag ttccttacag atttcttcat tatccatttc gtcgacatcg
     2461 cgcatggtga aaatggccgc caatttctgc ggtaaacgtt ccatgcactg ctgtaacact
     2521 ttcaaaaact gtgtttgctg caatgcgtca tgtggaatat cccaaacctg cggtttttca
     2581 gcccaatgcc cagtgtcatc aaaaaaatca tccatattgg catcttcttc actaagcagt
     2641 tcagagaccg cggtctcgcg cacctgtttg cgcatgcaat caatgatttt gtgtttgaga
     2701 atgccggtta accaggtgcg tgggctagat tgttcggcaa agttagggtt tttaatggct
     2761 gcgagtaaag tctcttgcac cacatcttct gcctgatgcg ggtcccgcaa ccttgccagt
     2821 gcaaagcggt agagatagtc accgtgctca ttgagccaat catgcgggtt agttgccatg
     2881 gcggctattg aagcataatt taggcagagg gggcaatgcc ggcgcgttgt tcaatcaagt
     2941 tcaggtaatg tgccgcatcc aagcccgttt gattgcgttg cgccagccag atagtttcgg
     3001 ccaaacattc gagtagtgca tgttgggctt cgtgttcttc ctggaagcgt gtgcacagtt
     3061 tgccataagc aggggtgatg ccaattggct ggttgatgct gatttgctct tgaatggaaa
     3121 gatgcaaact catatgcaag aaggggttgg tttcgcccat ttccggataa taggtctgtt
     3181 caaggtattg ctcgggatga tccaggatcg catggtattc aggatgcata tgcatgatgc
     3241 caacggccat tttttcaagc tcggtcagca tgtgctgctg tttgaatttg gcccaggcat
     3301 caaagaaaaa ctggcggact tcgtcacggc tcggattaaa tagggccata ctcacgctcc
     3361 tgccggtggg gccaccggat atatttgctt atcgtgaaac tcacataaat cggcaatgca
     3421 acactctccg catttcgggt tgcgggcaat acacgtgtaa cgtccatgca aaatcagcaa
     3481 gtggtgggcg tcttttaaaa actgcttggg agtcgtgctc aaaaagcgtt tttcgacttc
     3541 catcacgttt tttcccggtg ccagcttggt gcggttgccg acgcgaaaca aatgtgtatc
     3601 aacagcaatg gtgggctggc caaatgctgt attcaggatg acgttggctg ttttgcgccc
     3661 cacgccagct aatgcctcca gtgcttcacg attgtctggc acctcgccgc catgcaaagt
     3721 aattaattgg tggcagcatt tgagcacatt attcgccttg gcatggtaaa ggcctatggt
     3781 cttgatgtgg ctttccaggc cttccaggcc caaatccaga atcgcttgtg gtgtattagc
     3841 gaccttgaac agcttgcggg ttgcgatatt cacgccttta tctgttgcct gtgcggacag
     3901 aatcacagca atcagtaact cgaatgtgga gctgtgttcc agctctgtgg tcgggttggg
     3961 aatggcttgt gccagccgct caaatattgc cagtcttttt tgtgcattca taaaacggtt
     4021 tcaatcatcg gtcacaggga ttagcctgtc ttttgtgctt tgacacgtgc catcgcggca
     4081 gcaagggctg tttttttgag tgagtcagtg tttgcctgaa cctcaggtat ttctggtgtc
     4141 agtttgtgct tattgtaagt gtgggctttt gcctgctttt caagcgcgag gcgggccagt
     4201 cgttgttggt ggcgtcgcct ggcttggtcc gcttccgttt tgctcatggc gactggtcgg
     4261 ccgggaatag gctgcatgtc tatgcaatcc accggacagg gcggtaaaca tagctcacag
     4321 ccagtgcact cctgagtaat cacagtgtgc atgagtttag atgcgcccaa gatggcatcc
     4381 accggacagg cctggataca caaagtacag cctatgcatg tttcttcgtc aatccaagcc
     4441 accgcttttg gtttagtgac gccatggact ggatttaggg cttggtaggg acgttgtagt
     4501 agttgggcaa gtgcatgaat gccagcttcg ccacccggcg ggcattgatt gatattggct
     4561 tccttgcggg cgatggccca ggcatatggt ttgcagccat cataaccaca ttggcggcat
     4621 tgggtttgtg gcaatagcgc gtcgatctgc acaaccaagg cttccaatcc ggaggtgtct
     4681 tgtcctgatg tctggtcgtg gcggggtgtt tgcatcctgt cgcgcaaagg tgttctttag
     4741 tcaatttgga taagcggtat tataactgaa agcgtatagc cctatggcta gggctaagtg
     4801 ctcgctactg atagattgag tgactgtcca accctgtgag ttgccagcat tggctggatg
     4861 aggcattctg tgactagctg cgcgtgttct ttggcgttcg ggcggataat cagttaaaat
     4921 attcgcttta ttcttaatat ggcgctaggt atgttaacgg gcagtttggt cgcaatcgta
     4981 actccaatgt ttgaagatgg acgtttggat ctggacgccc ttaaaaagct catcgacttt
     5041 catgtagatg caggcacgga tgggattgtc attgtcggta cgactggcga gtcgccaacc
     5101 gtggatgtgg atgagcattg cttgctgatc aagactacga ttgagcatgt tgccaagcgt
     5161 gtccctgtca ttgccgggac tggtgcaaac tctacagcag aagccatcga actgaccgcc
     5221 aaagccaaat cactgggtgc cgatgcctgc ctgctggtcg ccccctatta caacaagcca
     5281 tcgcaagaag gcttgtacca gcattttaaa gcggtcgctg aagccgtcga tattccacag
     5341 attttgtaca atgtgcctgg acgtacaggt tgcgacctga gcaatgagac tgttctacgc
     5401 ttggcgaaaa ttcctaatat tgtggggatt aaagatgcta ccggcggcat agagcgtggc
     5461 acagacttgc tgttgcgtgt gccagcagat tttgccgtct atagtgggga tgatgcgaca
     5521 tcgctggcat tgatgttgct cggaggcaaa ggggtgattt cagtcacggc caatgttgct
     5581 cccaagttaa tgcatgagat gtgcgagcag gctctaaaag gcaacctggc cgccgccaag
     5641 gcagcgaatg ccaaattgtt tgccttgcat caaaagttgt ttgtcgaggc caacccaatt
     5701 cctgtgaaat gggtattaca acaaatggga ttgattggca ccggtattcg tttgccactg
     5761 gtcaatttgt ccagccaata tcatgaggta ttgcgcaatg ccatgcagca ggcagagatt
     5821 cctgcttgaa tgggctaaca ctaatttagg atgaaataag tgaaatacat gagtcatgtt
     5881 tggttacaac gtgttgtgct ggccagtctg gtcactgtgc tggcagcttg cgattccatc
     5941 ccgtttattg ataacagttc tgattacaag ggcgctggcc gttccaaacc actggaagtg
     6001 ccgccagacc tgaccgccgt acgtaccagc agtacttata atgtgcctgg cagcaccagc
     6061 tactctgcct acagccaggg ccaggaagcc caggagcaga atgggccgca acccgttttg
     6121 gctgacatga aaaacgtgcg tatggtgaaa gctggccagc aacgctggct ggtggtcaat
     6181 gcaccgccgg aaaaaatctg gccgattgtg cgtgactttt ggctggatca agggttcgcg
     6241 gtgagggtgg agaatcctga gcttggtgtg attgaaactg aatggctgca atccgatgcc
     6301 atcaagccca aggaagataa ccgtggctat ggtgaaaaat tcgatgcctg gctggataaa
     6361 ctatctggtt ttgctgaccg ccgtaaattc cgcacacgcc tggagcgcgg cgagaaagac
     6421 ggtaccactg aaatctatat gacgcatcgt accgtcgcag gtgcaccaga tgatggcaaa
     6481 aactatgtgc agacccaact gggtgtcatt gatacaggct accgtaaaga tgcagcagaa
     6541 aataaaaaca atgccgggca ggagtttgat gctgatctgg atgccgagtt gctacgccgt
     6601 atgatggtga agctgggcct ggatgaacaa aaagctgatc aggtcatgac gcaagcggct
     6661 tctgacaagc gtgcagatgt cgtcaaagaa tcagatcaga gcgtgacctt gaaactcaat
     6721 gagccatttg atcgcgcctg gcgccgtgtc ggtctggcat tggatcgtat tggttttgtg
     6781 acggaagata aaaaccgttc agaaggcatt ttctatgtgc gctatgcgga tacagatgca
     6841 gaagatccaa tcaagcagaa gaaaggcttg ctggagcgct tgaagttctg gggcagcgac
     6901 gatgacgata agagcacgac acctaaagat gtgaagccag gggataaaac caaattctgg
     6961 aagggaacat ctgacggcga taaatccagc aagcaatacc atatccaggt gctggaaaat
     7021 gcggatgaca ccacggatgt ctatgtgcta acgcctgaaa acaaacgcaa tacttccacc
     7081 acagcgaacc gt
//
LOCUS       DATMSK010000078         9054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_405961,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000078 DATMSK010000000
VERSION     DATMSK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9054
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405961"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VNZ84_02740"
     CDS             <1..820
                     /locus_tag="VNZ84_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HWU65898.1"
                     /translation="TDPSSGAGVQADIMALASIGCYPLSVITGITVQDTVGVENIMPL
                     DADLINEQARTILEDVSVSAFKLGLLGSVENVAVIAEIVADYPDVPLIIDPILASGRG
                     DELSNEEMQAAMCELLFPQATLITPNSLEARRLAYYDEGDEVKHSSLEECAMRLLGMG
                     TEYVMITGTHERSLDVVNSLYGMQNEVPGLIKDYHWERLPGSYHGSGCTLTSAITACL
                     AHGLSMEEAVHEAQEFTWQTLRHAFRPGMGQFIPDRFFWARESDVQEKASELQH"
     gene            804..1430
                     /gene="thiE"
                     /locus_tag="VNZ84_02745"
     CDS             804..1430
                     /gene="thiE"
                     /locus_tag="VNZ84_02745"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWU65899.1"
                     /translation="MNFSISGLYLVTPDCDDTTVLCAQVGQALQGGVALVQYRHKTAS
                     ESLRLEQASALLALCRRASVPLLINDHVALAMQLDADGVHVGQHDSAITTARAVLGNS
                     KIIGASCYNRFELAEKAQAAGADYVAFGACYPSSTKPHAPKASAALFTQAKQSLSLPV
                     VGIGGITLDNAPPLTEAGADALAVITDIFQAADIAARCRQYQSLFVKN"
     gene            1502..2872
                     /gene="mpl"
                     /locus_tag="VNZ84_02750"
     CDS             1502..2872
                     /gene="mpl"
                     /locus_tag="VNZ84_02750"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HWU65900.1"
                     /translation="MHIHILGICGTFMGGIAVLAKAAGHRVTGCDANVYPPMSTQLEA
                     QGIELIEGFDPSQTSLNPDIYVIGNVVARGNPLMEEILNQGLPYISGPQWLAENVLQG
                     KWVLAVAGTHGKTTTSSMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDAKSI
                     SPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFEDLPAIEKQFHHLVR
                     TVPQQGLVVANQQESLDRVIGRGCWSALERIDSASGWQVQHVDAQGRFDVYFKGERQG
                     NVSWELLGEHNRNNALAVIAAARHVGVAPCIAIEALSEFKNVKRRMEVKGIVNEVTVY
                     DDFAHHPTAIATTVAGLRAKVGAARILAVLEPRSNTMKLGVMKDALPESLRDADAVFC
                     YANNLGWDAAAALAPIQQKAQTFDELQQLVTAVTAMAKPGDHVLVMSNGGFGGVHQKL
                     LQALQG"
     gene            2888..3844
                     /locus_tag="VNZ84_02755"
     CDS             2888..3844
                     /locus_tag="VNZ84_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWU65901.1"
                     /translation="MGFRDSFRKRSPLVWALWASVLAHAVVLTLHFQPELKALKDQIP
                     SLEVMLVNSKTQSAPEKADALAQANLDRGGNTDEKRQMKSPLPSVQQMQASMPNVAQA
                     TLPSTTTASSPHEAELVREKQRVAQLEQEAQALLTQLKASQSVTSLQTPPLSQPYPAS
                     KQQPPSQHLPVQPQPQTAATQEMAKLEALIAKQQEEYEMRPKRQFIGARTREYRFANY
                     VEQWRQKIERVGNLNYPQTAVTQKLYGKLQMTVSVRADGSIESIRIHQGSGHRLLDDA
                     ARRIVQLAAPFPVFPEDIRQDTDVLSITRTWTFTRDDQLFTE"
     gene            3879..4193
                     /locus_tag="VNZ84_02760"
     CDS             3879..4193
                     /locus_tag="VNZ84_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWU65902.1"
                     /translation="MTDSHFKYHVFFCLNQRENGEDCCMNKGAEAAFDYMKKQVKQKG
                     LAGAGKVRINRAGCLDRCGEGPVMVIYPDAVWYTFIDESDIDDIIESHLAQGKVVERL
                     LV"
     gene            4203..5456
                     /locus_tag="VNZ84_02765"
     CDS             4203..5456
                     /locus_tag="VNZ84_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HWU65903.1"
                     /translation="MLKLWCKRLVATVLITCSLAVLGVAAWIWQPYDHTAWRVKLPVG
                     SGIQVRVLPMLMLATSPAGRWLLDKKGFSLHHGDIRLYDADGLRVHCQHCWLEAKSVS
                     DKPLMLDSVELWLKLDGQQLTGYLLADAATKPFKIYFDGTVTMRSLRLRWTLPTTPLA
                     SLLAPLRQHSPVIKQASVSGVLGAEGTLRWPKQRWSAQPQLEQLQVSGLDLSKVTTSS
                     VQYDCPLLDEQKHPEKVHWLSYDKMGRWLPMATIIAEDAEFRHHPGYAIAQMQQLLGK
                     ETADKAVGGSTITQQVVKYMFTNGERTWKRKIEELLYAVQLEYTLDKTQILNLYLNTV
                     DWGPGLCGAYAAANYYFGVEPAQLSPIQAAWLAGTIRNPHRAWKQQFIAQQPQLARAE
                     SILQYMPESARKQPGSLIFRATTAK"
     gene            5502..6581
                     /locus_tag="VNZ84_02770"
     CDS             5502..6581
                     /locus_tag="VNZ84_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HWU65904.1"
                     /translation="MSTLKLNKSPAPADQKRQKSVRRMSPELRDRSKAPVVKKSSQPV
                     VSQEGNTSVQAPRKDVGARKEHAADKKPIGKPAQGGARSANDTRRREKPREQERRTFT
                     EKSPARPVRPAPDVDAQPRLSKVMADRGLCSRREADDWIVNGWVKVNGEIVETLGTRI
                     DADAEIVVSAYAKEHQAENVTILLHKPIGYVSGQAEDGYEPAVVLVKAENQWAEDPTQ
                     KAFRKGFLHGLAPAGRLDIDSTGLLVLTQDGRVARQLIGDDSTTEKEYLVRVEGQLSA
                     NGLAKLNHGLSLDGVQLKPAKVSWQNEDQLRFVLREGRKRQIRRMCELVGLKVVGLKR
                     IRIGRIALGKLPPGQWRYLGAHERF"
     gene            6615..7004
                     /locus_tag="VNZ84_02775"
     CDS             6615..7004
                     /locus_tag="VNZ84_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HWU65905.1"
                     /translation="MGLFDSVAGAMMNKVMGDKGPLAKLAMELFQQYGGLPGILQTLK
                     NGGLTEQVDSWVGKGANLNVSAQQIGAALGSSVLTGIAGKLNMTTDELTGKIAEHLPD
                     VVNQLTPNGVVENNPALIMSRLMGMLK"
     gene            complement(7049..7696)
                     /gene="pyrE"
                     /locus_tag="VNZ84_02780"
     CDS             complement(7049..7696)
                     /gene="pyrE"
                     /locus_tag="VNZ84_02780"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWU65906.1"
                     /translation="MTAHTLSADFIAFSIHKQVLRFGEFKTKAGRLSPYFFNAGLFDD
                     GESMLKLGEFYANSILQSGVQFDMLFGPAYKGIPLVTAISIAFARMGKNYPYAYNRKE
                     AKDHGEGGTLVGAALKGRVLIIDDVISAGTSIRESVELIRNAGATPCAVSIALDRQEK
                     GLGELSAVQEVEQTNQMPVVSIAKLADLFAYLQGNEKLAQYFPAIEAYRGQYCVR"
     gene            7723..8496
                     /locus_tag="VNZ84_02785"
     CDS             7723..8496
                     /locus_tag="VNZ84_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWU65907.1"
                     /translation="MRIITLNLNGIRSACNKGFLPWLANSGGDIVCLQELKAQVGDMQ
                     PEMLQPPGYHGYFHYAEKKGYSGVGIYSKQPADKVIIGLGHTHIDAEGRYLECQYGNL
                     SVVSLYLPSGSSGEERQQFKFEVMALFLPHLEQLIASGREVVICGDWNIAHQEIDLKN
                     FKGNRKNSGFLPEERAWMTDVLGRVGWVDTYRGLYPDTTDECYTWWSNRGQAYAKNVG
                     WRIDYQLATPVLGAKVVAASVYKAERFSDHAPLIVDYED"
BASE COUNT         2259 a         2153 c         2522 g         2120 t
ORIGIN      
        1 aaccgatcct agtagtggtg ccggtgtgca ggcagatatc atggcacttg ccagtattgg
       61 ttgttacccg ctttcagtga ttaccggcat tacggtacaa gataccgtgg gtgtcgaaaa
      121 tatcatgccg ctagatgcag atctgatcaa cgagcaagcg cggacaatat tggaagatgt
      181 gtcggtcagt gcatttaaac tgggtttgtt agggagtgtc gaaaacgtgg cagtgattgc
      241 cgagatcgtc gcagactatc cggatgtgcc tttgatcata gacccgattc tggcctcagg
      301 ccgtggtgat gaactgtcta acgaggaaat gcaggcggcc atgtgtgagc tgctgttccc
      361 acaagcgaca ctcatcaccc ctaatagtct ggaagcacgt cgcttggctt attacgatga
      421 gggtgacgaa gtgaagcata gctccctcga ggaatgtgca atgcgattgc tcggaatggg
      481 caccgaatat gtgatgatta cgggtacaca cgagcgttca ctggatgttg tgaatagctt
      541 gtatggcatg caaaatgagg tgccagggtt gatcaaagat tatcattggg aacgcttgcc
      601 gggtagttat cacggctctg gttgcacatt gaccagtgcg attactgcct gcctggcgca
      661 tggcctgagt atggaggagg ctgtgcacga agcgcaggaa tttacctggc aaaccttgcg
      721 tcatgccttc cgtcctggca tgggtcaatt cattcctgac cggttcttct gggcacgtga
      781 atctgacgtg caggaaaagg ccagtgaact tcagcattag cggtttatac ctggtcacac
      841 cggattgtga tgacaccacg gtactatgtg cacaagtcgg gcaggcgttg caaggtggtg
      901 tggcattggt gcaataccgt cataaaactg ccagtgaatc tttacgcctg gagcaggcct
      961 ctgccctgct cgcactatgc cgtagggcaa gtgtgccttt gctcatcaat gatcatgtcg
     1021 cactagccat gcaacttgac gctgatggtg tgcatgtggg ccagcatgac agcgcaatta
     1081 ccactgcacg cgcagtatta gggaattcaa aaatcattgg ggcttcttgc tataaccgct
     1141 ttgagcttgc agagaaggct caggcagcag gcgctgacta tgtcgctttt ggtgcctgtt
     1201 atccctcttc taccaaaccg catgccccaa aagcaagtgc cgcgctattc actcaggcaa
     1261 aacagtcctt gtcgctgcct gttgtcggca ttggcggcat taccctggat aatgctcccc
     1321 cattgactga ggcgggtgcc gatgcactgg cggtgattac cgacattttc caggctgcgg
     1381 atattgcagc acgttgccgc caataccaat ccctgtttgt aaaaaattga gcagttatcg
     1441 ctaccgcttt accaggcgtt gtttggtaaa atgcatgcgt taaactgttc tggtaaattt
     1501 tatgcacatt cacatccttg gtatttgcgg cacttttatg ggcggcattg ccgtactggc
     1561 gaaagcggcc ggacaccgtg tcaccgggtg tgatgcgaat gtttacccgc ccatgagcac
     1621 gcaactggag gcgcaaggca ttgagctgat cgaagggttt gaccctagcc aaaccagcct
     1681 gaatcctgat atctacgtca taggcaatgt ggtggcgcgc ggcaatccac tcatggaaga
     1741 aatactcaac cagggtttgc cttatatttc tggcccacaa tggctggccg aaaatgtact
     1801 gcaaggcaaa tgggtactgg cagtggctgg tacacacggt aaaacgacga cttcctccat
     1861 gctggcatgg gtgcttgaat acgcagggct ggcgcctggt ttcctgattg gtggtgtgcc
     1921 agagaacttt ggcgtgtctg cccgcttgcc gcaaacgcca cgacaagatg ccaagtcaat
     1981 ttcaccgttt tttgtgatcg aagcggacga atacgatacg gcttttttcg acaaacgctc
     2041 caaattcgtg cactaccgtc cgcgtaccgc tgtgctgaat aatcttgagt ttgaccatgc
     2101 ggatattttt gaagacctgc cggcgattga aaagcagttt caccatctgg tacgcactgt
     2161 accgcagcaa gggctggtgg ttgccaacca gcaagagagc ctggaccgcg tgatcggacg
     2221 tggttgctgg agcgcattgg aacgcataga tagcgccagt ggctggcaag tgcaacatgt
     2281 tgatgcgcaa ggccgatttg atgtctattt taagggtgag cgtcaaggga atgtgagttg
     2341 ggagttgctt ggtgagcata accgtaataa cgcgttggcc gtcattgcag cggcgaggca
     2401 tgtcggtgtt gcaccctgta ttgcgattga agcgttgagt gagtttaaaa acgtcaaacg
     2461 ccgcatggag gtcaagggca ttgtcaatga agtgaccgtt tatgatgact tcgcccatca
     2521 cccgacagcc attgcaacca cggttgcagg tttgcgcgcc aaagtgggcg cagcccgtat
     2581 tctggcagtg ctcgaaccac gttccaatac gatgaagctg ggggtgatga aagatgcact
     2641 gccagaaagc ctgcgtgacg ccgatgccgt attttgttat gcgaacaatc ttggttggga
     2701 tgccgccgct gcattggcac ccatccagca aaaagcgcaa acctttgatg agctgcagca
     2761 actggtgaca gcagtcaccg cgatggcaaa accgggtgat catgtgctgg tcatgagtaa
     2821 tggcggcttt ggcggcgtac accagaaact gttacaagcc ctgcagggat aaacaggcag
     2881 atacccaatg ggtttccgcg atagtttccg taaacgttcg ccgctggtct gggcattgtg
     2941 ggcctcggtg ctggcgcatg ccgtcgtgtt gacgctacat ttccagcccg aactcaaggc
     3001 actcaaagac cagattcctt cattggaagt catgctggtt aacagcaaaa cacaaagtgc
     3061 gcctgagaag gcagatgcgc tagcgcaagc caatctggat cgcggcggca atacggatga
     3121 aaagcggcag atgaagagcc cgctgcccag tgtgcagcaa atgcaagcct ccatgccaaa
     3181 tgttgcacaa gcaacgttac catcgacaac gacggctagc agtccgcatg aggctgaact
     3241 ggtgcgtgaa aagcaacgcg tggcccagtt ggagcaagag gcccaagcgc tgttaacgca
     3301 gttaaaggcc agtcaatcgg tgaccagcct gcaaacaccg ccactgtcgc aaccttaccc
     3361 agcatctaag cagcaaccac catcgcaaca tctacccgtg cagccccaac cgcaaacggc
     3421 ggccacgcaa gaaatggcca agctggaggc gctcattgcc aagcaacagg aagagtacga
     3481 gatgcgtccc aagcgccagt ttattggcgc acggaccagg gaatatcgtt ttgcaaatta
     3541 tgtcgaacag tggcggcaga aaattgagcg ggtcggtaac ctcaattatc cacaaactgc
     3601 ggtaacccaa aagctttatg gcaagttgca aatgacagtt tcggtgagag ccgatggtag
     3661 tatagaaagt atccgtatcc atcaaggttc cgggcatcga ttgctggatg acgcagctag
     3721 acgcattgtc cagctggcgg ccccttttcc ggtgtttccg gaagatatcc gccaggatac
     3781 agacgtattg agcattacac ggacttggac ttttaccagg gatgaccaac tcttcactga
     3841 atagctttat tcggtgcaag ttatacaagg caattgctat gaccgatagt cactttaaat
     3901 atcatgtatt tttttgtctg aatcaacgcg agaatggcga agactgttgt atgaacaaag
     3961 gggctgaagc cgcgtttgac tacatgaaga aacaagtcaa gcaaaagggg ttggccgggg
     4021 caggtaaagt ccgtatcaat cgtgccggtt gcctggatcg ctgtggtgaa ggcccggtca
     4081 tggtgattta cccggatgcc gtttggtata catttatcga tgaaagtgac atcgatgaca
     4141 tcatcgaaag ccacctggca caaggcaagg tggtggagcg tttgctggtt taaggtgggt
     4201 tcgtgttaaa actctggtgc aaacgccttg tcgcgacggt gttgattacc tgttctctag
     4261 cagtgctcgg tgtggcggcc tggatttggc agccctatga ccatacggcc tggcgggtga
     4321 aattacctgt gggcagcggg atacaagtgc gtgtgttacc tatgttgatg ctggcgacct
     4381 ctcctgcggg ccggtggtta ctggataaaa aaggcttcag cctgcatcac ggcgatattc
     4441 gactgtatga cgcggatggc ttacgcgtgc attgccagca ctgttggctg gaggctaaat
     4501 ccgtcagtga caaaccactg atgctggatt cagtggagtt atggttaaag ctggatggtc
     4561 agcaattaac aggctatttg ctggcagatg cagcgaccaa accattcaaa atttatttcg
     4621 atggcacggt aaccatgcgc tctttgcggt tgcgctggac attgccaact acgcccttag
     4681 ccagtctatt ggcgcctttg cgccagcatt cccctgtgat taaacaagcg agcgttagcg
     4741 gcgtacttgg tgctgaaggg acgctgcgct ggcccaagca acgctggagt gcacaaccac
     4801 aactggaaca gctacaggta agcgggctgg acttaagcaa agtcactaca tcctctgtgc
     4861 aatatgattg cccattgttg gacgagcaaa aacacccgga aaaagtacat tggctgagct
     4921 acgataagat gggccgctgg ctaccgatgg cgactatcat tgccgaggat gccgagttca
     4981 ggcaccaccc tggttacgct atcgcacaaa tgcagcaact actgggcaag gaaacggccg
     5041 acaaggctgt tggcggcagt accatcacac agcaagtggt gaaatatatg ttcaccaatg
     5101 gtgagcgaac ctggaagcgg aaaattgaag aattactata cgcagtccag ttggagtata
     5161 cgctggataa aacgcaaatt ctcaacctgt atctgaacac agtggattgg ggcccaggtt
     5221 tgtgtggcgc atatgcagcg gcaaactatt attttggcgt ggaaccggct caattgagcc
     5281 cgattcaagc ggcctggctg gctggcacta tccgcaatcc gcatcgggcc tggaaacagc
     5341 agtttatcgc gcaacagccg caactggcca gggcggaaag tattctgcaa tacatgccgg
     5401 aaagtgcgcg taagcaacct ggcagcctga tttttcgtgc gacgacagcc aaataaatta
     5461 caatagcggc ttattcatct tttgttagtg agaagactgt tttgtctacg ctcaaactca
     5521 ataaatcccc tgctcccgct gatcagaaac gacaaaaatc agtcaggcgt atgtcgcctg
     5581 agctacgcga ccgtagtaaa gcacctgtcg ttaaaaagag cagtcagcca gtagtttccc
     5641 aagaggggaa tacttcagtg caggcaccgc gcaaagatgt tggcgcgcgt aaggagcacg
     5701 cagcagataa aaagcccatc ggcaagcctg cacaaggcgg agcgcgctct gcaaatgata
     5761 ctcgccgtcg agagaaaccg cgggagcaag agcgacgtac gttcacagaa aaatcgcctg
     5821 cccgtccagt ccgccctgcc cctgatgttg acgcccaacc gcgcttatcc aaagtgatgg
     5881 cagaccgcgg cttatgctca cgccgtgagg ctgatgactg gatcgtcaat ggctgggtca
     5941 aggttaatgg tgaaattgtc gaaacactgg gaacgcgcat tgatgctgac gcagagattg
     6001 tggtgagtgc ttacgccaaa gagcaccagg ctgagaacgt gactattttg ctacacaagc
     6061 cgataggtta tgtcagtggg caagccgaag atgggtatga gcctgcagta gtgttggtga
     6121 aggcagaaaa ccaatgggca gaagacccga cacaaaaagc attccgtaag ggcttccttc
     6181 atggattagc cccggctggc cgcctggata ttgattctac cggactgtta gtgctcactc
     6241 aggatggtcg cgtcgcgcgc cagctgattg gcgatgattc gacgactgaa aaagaatatc
     6301 tggtgcgcgt tgaagggcaa ctgagtgcca atggcctggc taaattgaat catggcttga
     6361 gtctggatgg tgtacaactg aaaccagcca aagtgtcctg gcaaaacgaa gatcaactgc
     6421 ggtttgtgtt acgcgaaggc cgcaaacggc agattcgtag gatgtgtgag ttggtgggct
     6481 tgaaagtggt gggtttgaaa cggatccgta ttggacggat cgctttaggc aaattaccac
     6541 ccggccagtg gcggtatttg ggtgcgcacg aacgcttttg atcaagctgt aatgaagatt
     6601 atttaggaac cataatggga ttatttgata gcgtcgctgg cgccatgatg aacaaggtga
     6661 tgggcgacaa aggcccttta gcaaaactcg ccatggagtt gtttcagcaa tacggtgggt
     6721 tgccgggtat tttgcaaacc ctgaaaaatg gcgggttgac cgagcaggtc gattcgtggg
     6781 taggaaaagg ggccaacctg aatgtcagcg cacagcaaat tggtgctgct ttaggcagtt
     6841 cagtactgac tggtatcgca ggcaaactga atatgacgac agacgaactg actggcaaaa
     6901 ttgccgagca tttgccggat gtcgtcaatc agctcacacc caatggtgtc gttgaaaata
     6961 atccggcact gatcatgtcg cgcctgatgg gcatgctgaa ataaaataaa gcgagcgcag
     7021 gctcgcttta ttctttgtga aaagcttact agcgcacgca atactggccg cgatacgcct
     7081 caatggcagg gaaatattgc gccagtttct cgttaccttg caagtaggca aataggtcag
     7141 ccagtttggc gatgctgacc accggcatct ggttggtttg ctcgacctct tgcacggcgg
     7201 agagttctcc caggcctttt tcctgacggt ccagcgcgat actgacggca caaggggtgg
     7261 ctccggcgtt gcggatcagt tcaacagatt ctctgataga agtgccagca gaaatcacat
     7321 catcaataat cagcacacgg cccttgagtg ctgcgccgac tagcgtgccg ccttcgccgt
     7381 ggtctttggc ttctttacgg ttataagcat aaggataatt tttacccatg cgcgcaaagg
     7441 caatgctgat ggccgtcacc aacggaatgc ccttatatgc cgggccaaac agcatgtcga
     7501 attgcacgcc agactgtaga atgctgttgg cgtagaattc acccagtttg agcatgcttt
     7561 cgccgtcatc aaacaggcct gcgttgaaaa aataagggct caagcggccg gctttggttt
     7621 taaattcacc aaagcgcaac acctgtttat ggatggaaaa agcaataaaa tcggcactga
     7681 gtgtgtgggc agtcatttta aaaaagcaga aagtattaaa caatgcgtat tataaccttg
     7741 aacctgaatg gcatacggtc agcctgtaac aaaggctttt tgccatggct ggccaatagc
     7801 ggcggcgata ttgtgtgttt gcaagagctc aaagcccaag tgggcgacat gcagcctgag
     7861 atgttgcaac cacccggtta tcacggctat ttccattatg ccgagaaaaa aggctacagc
     7921 ggcgtgggta tttacagcaa acaacctgcg gacaaggtga ttatcggcct gggtcatacg
     7981 cacattgacg ctgaaggccg ctacctggaa tgccagtatg gcaacctgag tgtggtgtct
     8041 ttatacctgc caagtggatc cagcggcgaa gagcgccagc aattcaagtt tgaggtcatg
     8101 gcattatttc taccgcatct tgaacagttg attgcttctg ggcgtgaagt cgtgatttgt
     8161 ggtgactgga atattgcaca tcaggagatt gatctcaaga acttcaaagg taaccgcaag
     8221 aactcaggtt tcttgccaga agagcgcgcc tggatgacgg atgtgctagg acgtgtgggt
     8281 tgggtggata cttaccgtgg cttatatcca gacaccacag atgaatgtta tacctggtgg
     8341 agtaatcgcg gccaggcata tgccaagaac gtcggctggc ggattgatta ccagttggca
     8401 acgccggtgt tgggtgcaaa agtggttgcg gcgagtgtgt acaaggcgga gcggtttagt
     8461 gaccatgcac cgttgattgt ggactatgag gattgagtaa ggtaggtctt tcttttagct
     8521 taattgtaag cttcaccatt ctttttcgcc tgtaggcgag ttactttctg ttgcttgccc
     8581 cacagaaagt aaccaaagag gagggcaccc agccatcacg gcctaacggc tgccttgcgt
     8641 tgctcaacaa agtaagcggt cacgaaactc gccctagcaa cttgtacact acgcaagctg
     8701 ccgcggagct caaacagtcg ctcccttatt tcttactttg tttccgctac tcagcgtgat
     8761 ggaatgggac ttttctcatt taagcagcgc tgttgaattt tgaacggttg taattcaaga
     8821 tcacaaaggc tggcactcat ggtttttgcg ggcccctatc tgccgcgccg agtagcgcag
     8881 gcaaaataag gagaaaggga gcgactgtgc gaatcccgca gtagctcacg ttgtgtgtga
     8941 gctaccaggt taaatttcgt gaccgcttat tttggcgagc aacgcaggaa acaagcggaa
     9001 gctagggtgt cttgttcttt gccttctttc ttatggacaa gcataagaaa gaac
//
LOCUS       DATMSK010000079         7198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_453141,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000079 DATMSK010000000
VERSION     DATMSK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453141"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..494
                     /gene="rimP"
                     /locus_tag="VNZ84_02790"
     CDS             6..494
                     /gene="rimP"
                     /locus_tag="VNZ84_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWU65908.1"
                     /translation="MQNLHSVVEKTVTQLGYELVDFEAANRGKLLRVFIDKLTPKDNK
                     DSVTIDDCVLVSNQLGNVLTVELDIDYDRLEVSSAGLDRVLRKEGDFVRFAGERAQIK
                     LRVGIQDDGPKATATSLPRKTFLGLLQGVEEGKVVIECDDWVYRLSLDNIDKARLSPV
                     FD"
     gene            534..2000
                     /gene="nusA"
                     /locus_tag="VNZ84_02795"
     CDS             534..2000
                     /gene="nusA"
                     /locus_tag="VNZ84_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWU65909.1"
                     /translation="MSREILLLVDALAHEKNVDKEVIFTALELALASATKKNHEEGAD
                     IRVEIDRESGDYKTFRRWQYVEYDLLESSAYQFDEEDERAAGRQIGDYYEEPLESIAF
                     GRIGAQAAKQVILQKVREAEREQMIQDFLARGESLVTGVIKRMEKGNAIIEVGRIECL
                     LPREAMIQKENLRVGDRVRAYLSRVDRGGRGPQLILSRVAPDFLKRLFELEVPEIEEG
                     LLEIRSAARDPGLRSKIAVKTNDQRLDPVGTCVGMRGSRVQAVTSELGGERVDIVLWS
                     MDPAQFVINALAPAEVTSIVVDEDAHSMDVVVNEEQLAVAIGRNGQNVRLASELTGWN
                     LNILTEEAAAQKSQDEYAKISQLFIEKLDVDEEVADILVQEGFSTLEEIAYVPLAEMS
                     EIEAFDEDTINELRSRARAALLTEAIAKEEKVEEDTNDLMTLEGMDADTAHKLAANEI
                     HTSEDLAELAVDELIELTGIDEERAKSLIMTARAPWFK"
     gene            2067..4763
                     /gene="infB"
                     /locus_tag="VNZ84_02800"
     CDS             2067..4763
                     /gene="infB"
                     /locus_tag="VNZ84_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWU65910.1"
                     /translation="MALSTVAQFAAELGLPTTLLLEQLKSAGVHKTSVDDFLEEGDKA
                     ALLDYLRKEHGAGNAPKGKITLTRKQNTEIKKLDSTGKARTIQVEVRKKRVLERPEIG
                     YDQPVETAAFEEVEEIAPEVLEELPVAVEEVAEQPPVEETPEVLPEPEPETVESPATE
                     AAPVVEAPAIAKKAVVLSAQEIALREQEAARAAKLAALQAEDVKRKQALTQRRAEEEA
                     KRQAEAEAAKNKPAATKLSEGTLHKPAAKATDKPENKDKKAKTDNRDWNENENKKRGI
                     KTRGDSAGGANQGWRTPKGKHHKSSKDDQHAFNAPTEPIVKEVMVPETISVADLAHKM
                     SVKAAEVIKALMKMGMMVTINQVLDQDTAIIVVEEMGHKAQAAAANDPESFLEETDTV
                     EAVMEARPPVVTVMGHVDHGKTSLLDYIRRSRVASGEAGGITQHIGAYHVETPRGMVT
                     FLDTPGHEAFTAMRARGAKATDIVILVVSADDGVMPQTIEAIHHAKAGNVPIVVAINK
                     IDKPEAQPERVKNELITHEVVPEEYGGDTMFREVSAKTGKGIDELLEAVLLQAEVLEL
                     KAPLNTPAKGLVIEGRLDKGRGPVSTVLVQSGILRRGDMLLAGTAFGRVRAMLDESGN
                     DIKEAGPSIPVEVLGLSDVPSSGEETIVLNDERKAREIALFRQGKFRDVKLAKQQAAK
                     LENMFEQMAEGEVQTLNLIIKSDVQGSFEALTTSLQKLSTSEVRVNIIHTAVGAISES
                     DVNLAAASKAVLIGFNVRADAGARKLVETLDVDLRYYNIIYEAVDEIKAALGGMLAPE
                     QKEQMIGTVEIREVFKISKVGSIAGCYVQDGVVKRNSKVRLLRNNVIIHTGELESLKR
                     FKDDVKEVKNNFECGLSLKNYNDIEVGDILEVYEVVEVARTL"
     gene            4763..5119
                     /gene="rbfA"
                     /locus_tag="VNZ84_02805"
     CDS             4763..5119
                     /gene="rbfA"
                     /locus_tag="VNZ84_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWU65911.1"
                     /translation="MAKAFSRNDRVSEQMRRELADLLMFEVKDPRVQMVTLTGVEVAG
                     DMAHAKVFYTAQKNSKGLQAGLEKAAGFLRSQLGKRMMIRTVPQLHFVYDESIDRGMH
                     MDKLIHDALSDTPPQE"
     gene            5138..6037
                     /gene="truB"
                     /locus_tag="VNZ84_02810"
     CDS             5138..6037
                     /gene="truB"
                     /locus_tag="VNZ84_02810"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWU65912.1"
                     /translation="MQTKRIKRVVNGVLLLDKPYGFSSNQALQKVKWLYQAQKAGHTG
                     TLDPLATGVLPICFGEATKFAQHLTDENKTYIATVRFGVMTTTGDKEGDVISQVDCQV
                     TRKDIEQVLPQFMGEITQVPPMYSALKVNGKPLYEYARDGVELERAPRQVTIHALRVL
                     SCADNHAEIEVCCSKGTYIRTLGEDIGKALNVGAHLTALRRTATATYQIAQTVTIEAL
                     EQMAIEQKDALLLPVDTAITALPKIELHEDAAYYFKQGNPVWVSGNIPAGELRIYSET
                     GLFLGVGQQQRDGRIAPKRVVNL"
     gene            6118..7182
                     /locus_tag="VNZ84_02815"
     CDS             6118..7182
                     /locus_tag="VNZ84_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWU65913.1"
                     /translation="MRIPVSQALLMTLLLPLTVTPAEESDTRSSGNQRPAAMTMLYDA
                     RFELHDPGPGHPERASRVHAIVDQLKRNESLNPFLRWPAFSAATRADLLRVHSPAYLD
                     LLDKEQASVKPGQYHLLSTGDTMLSQHSLAVATLASGAVMAGVDAVMTAQSPVAFALV
                     RPPGHHATRDRGMGFCLTNHIAVATRYAQHKYGIQRILIIDIDVHHGNGTQDIFYADN
                     SVFYFSAHQHPLYPGSGRPSETGVGPGLGYTTNVDVPPGSNESRMLQAIERQLVPAMQ
                     QFRPELIMVSAGLDAHAGDTLGALQYSDAGYAALATATKQLAAHYTQGRMVWMLEGGY
                     VPANNANAVEAITRVLIAVP"
BASE COUNT         1870 a         1640 c         2039 g         1649 t
ORIGIN      
        1 gtcgcatgca gaatttgcat tcggtggttg aaaagacagt tacccagttg ggatacgagt
       61 tggttgattt tgaagcggcc aaccgcggca agttgttgcg tgtatttatc gacaagctga
      121 cacctaagga taataaagac agtgtcacga ttgatgattg cgtattggtg agcaatcagt
      181 tggggaatgt gctgacagtc gagctggata ttgattatga ccgcctggaa gtatcttcgg
      241 ctggtttgga ccgtgtgctg cgcaaggaag gtgattttgt gcgttttgca ggcgaacgag
      301 cccagatcaa attgcgcgtg ggtattcaag atgatggccc taaagccact gcaaccagtt
      361 tgccaagaaa aacattcttg ggtttgttgc aaggtgtgga agaaggcaag gtagtgattg
      421 agtgcgatga ctgggtttat agattgtcgc tggataatat tgacaaggct cgcttgagtc
      481 cggtctttga ttaatttgtt ttagctaatc cgtacggtga gttggagatt acgatgagtc
      541 gtgaaatatt gttgttggtt gatgccctgg cacatgaaaa gaatgtggat aaagaagtta
      601 ttttcactgc tttagagctg gcattggctt cggcaacgaa gaagaaccat gaagaaggtg
      661 ccgatattcg cgtcgagatt gatcgcgaaa gcggagatta caaaaccttc agacgttggc
      721 aatatgtgga atacgactta ttggaaagct ctgcttacca atttgatgaa gaggatgagc
      781 gtgctgcggg cagacaaatt ggtgattatt acgaagagcc attagagtct atcgctttcg
      841 gccgtattgg tgcgcaagcc gccaaacagg ttattttgca aaaggtgcgc gaagctgagc
      901 gtgagcaaat gatccaggac ttcctggctc gtggcgagag tctggtcaca ggcgtcatca
      961 agcgtatgga aaaaggcaac gcgatcattg aggtgggccg tattgagtgt ttgttgccgc
     1021 gtgaggcaat gatccagaaa gaaaacctgc gtgtaggtga tcgtgtgcgc gcttacctgt
     1081 cacgcgtaga ccgtggtggc cgtggcccgc aactcatctt gtctcgtgtg gcaccagatt
     1141 tcctcaagcg gttgtttgag ctggaagtgc cagaaatcga agaggggctg ctggaaatcc
     1201 gttcagctgc gcgtgatccc ggcttgcgtt caaagattgc ggtgaaaacc aatgaccagc
     1261 gcttggatcc agtgggtact tgcgttggga tgcgtggttc gcgcgtgcaa gcagtgacct
     1321 ctgagttggg tggcgagcgt gtcgatatcg tattgtggag catggatcca gcacaatttg
     1381 tgatcaatgc actggcgcca gctgaggtga cctccatcgt tgtcgatgaa gatgcacaca
     1441 gcatggacgt cgttgtcaat gaggagcaac tggcggtcgc cattggccgt aacgggcaaa
     1501 acgtgcgtct ggcgtctgag ctgactggct ggaacctgaa tatcctgacc gaagaagcgg
     1561 ctgcgcagaa gagccaggat gagtacgcta aaatcagcca gctatttatt gaaaagctgg
     1621 atgtagatga agaagtggct gacatcctgg tgcaagaagg ctttagcaca ctggaagaaa
     1681 tcgcttacgt cccactggca gaaatgtccg agatcgaagc ctttgatgaa gataccatca
     1741 atgaattgcg ctctcgtgcc cgtgccgcct tgttgaccga agcgattgcc aaagaagaga
     1801 aggtcgaaga agacaccaac gacttgatga ctctggaagg catggatgcc gataccgccc
     1861 ataaactggc ggctaacgaa atccatacca gtgaagatct ggctgaactg gctgtcgatg
     1921 agttgatcga gttaaccgga atagatgaag agcgtgccaa gtcattgatt atgactgccc
     1981 gcgcaccttg gtttaaataa cccatgcggt caagcatcat gcatacatct taattaaaaa
     2041 ataaagtaat tgaactggag cagtacatgg cactttcaac cgtcgcacaa tttgccgcag
     2101 aactgggtct gccaacgacc ctgctgctag agcaactgaa aagcgcaggc gttcacaaaa
     2161 cgtctgtgga tgatttcttg gaagaaggtg acaaggctgc cttgctggat tacttgcgta
     2221 aagagcatgg tgctggaaac gcgcccaaag gaaaaattac gctgacacgt aaacaaaaca
     2281 ccgaaatcaa gaagcttgat agtactggca aagcgcgtac tattcaggtg gaggtgcgca
     2341 aaaaacgcgt gttggagcgt cctgaaattg gctatgatca gccggtagaa actgccgcct
     2401 ttgaagaagt cgaagagatt gcgcctgagg tattggaaga gttgccagtg gctgtggaag
     2461 aggttgcaga acagcctcct gtcgaagaaa cgcctgaggt gttgcctgag ccggaacctg
     2521 agacagtgga atctcctgcg actgaagcgg cccctgtcgt tgaggcgcca gccatagcga
     2581 aaaaagcagt ggtgttgtcg gcgcaagaaa tcgccctgcg cgaacaagaa gcagcgcgtg
     2641 cagccaaact ggcggccttg caagctgagg atgtgaagcg caagcaagca ttaacgcagc
     2701 gtcgtgccga agaagaggca aagcgccagg ccgaagcaga ggctgcgaag aataaacctg
     2761 cagcgacaaa attgtctgaa ggtactttgc ataaaccggc tgccaaagcg acagacaaac
     2821 cggaaaacaa agacaaaaaa gccaagaccg ataaccgcga ctggaacgaa aacgagaata
     2881 agaaacgcgg tattaaaaca cgaggtgaca gtgctggcgg tgctaaccaa ggctggcgca
     2941 cccctaaagg taaacatcac aaatccagca aagatgatca gcatgcattt aatgctccga
     3001 ctgagccgat tgttaaagag gtgatggtgc ctgagactat ttctgtggct gacctggcac
     3061 acaaaatgtc tgtaaaagcg gcagaggtga ttaaagccct gatgaaaatg ggcatgatgg
     3121 tcacgattaa ccaggtgctg gatcaggata ctgccattat tgtggtggaa gaaatgggcc
     3181 ataaagctca ggctgcagca gccaacgatc cggaaagctt cctggaagaa accgatactg
     3241 tggaagccgt catggaagcc cgtccgccag tggtaacagt gatgggccac gttgaccatg
     3301 gtaaaacctc cctgcttgac tatatccgcc gcagccgtgt cgcgtctggt gaagcgggtg
     3361 gtattaccca gcacattggt gcttaccatg tggaaacccc acgtggcatg gtgactttcc
     3421 tggatacacc gggccacgag gcgtttactg ccatgcgagc ccgcggtgca aaagccaccg
     3481 atatcgtgat cttggtcgtg tctgccgacg acggcgtcat gccacaaacg attgaagcga
     3541 ttcaccacgc caaagccggt aacgtgccta tcgtcgtagc gattaacaaa attgataaac
     3601 cggaagcaca gccagagcgc gttaaaaacg aattaatcac gcatgaagtg gtgccggaag
     3661 aatacggtgg cgacaccatg ttccgtgaag tctctgccaa aacgggtaag ggcattgatg
     3721 agttgctcga agccgtattg ttgcaggcag aagtcctgga actgaaagca ccactcaata
     3781 cccctgctaa aggtttggtg attgaaggcc gtctggataa aggtcgcggt ccggtctcaa
     3841 ctgtgttggt acagtcaggt atcttgcgcc gtggcgacat gctgctggcg ggcactgcct
     3901 ttggtcgtgt gcgcgccatg ctggacgaga gtggtaacga tatcaaggaa gctggtcctt
     3961 cgattccagt tgaagtcttg ggcttgtctg atgtgcctag ttccggcgaa gaaaccattg
     4021 tattgaacga tgagcgcaaa gcgcgtgaaa tcgccttgtt ccgtcaaggt aaattccgtg
     4081 atgtgaaact ggccaaacag caagcagcca agctggaaaa catgtttgag caaatggctg
     4141 aaggtgaagt gcaaactctg aacctgatta tcaagtcaga tgttcagggc tctttcgaag
     4201 ccttgacgac cagcttgcag aaattgtcta ccagcgaagt acgcgtcaat attattcaca
     4261 ctgcggttgg tgcaatcagt gagtccgacg tgaacctggc agccgcttcc aaagcggtat
     4321 tgattggctt taacgtgcgt gctgatgcgg gtgcccgcaa actggtcgaa accttggacg
     4381 tggacctgcg ttactacaac atcatttacg aggctgtgga tgagatcaag gctgcgctgg
     4441 gtggtatgtt ggccccagag cagaaagagc agatgatcgg tacggtcgaa attcgtgaag
     4501 tcttcaagat ttccaaagtc ggttcgattg ccggctgtta tgtacaggat ggtgtggtta
     4561 aacgtaactc caaggtccgt ctgttgcgta acaatgtgat tatccatact ggtgagctgg
     4621 aatccctgaa acgcttcaag gatgacgtca aggaagtgaa aaacaacttt gagtgtggtc
     4681 tgtcattgaa gaattacaat gacattgaag tgggcgatat ccttgaagtg tatgaagttg
     4741 ttgaagtggc acgtactctc taatggctaa ggcattttca agaaacgacc gtgtctctga
     4801 gcaaatgcgc cgcgaattgg cggatttgct catgttcgag gtgaaagacc cgcgggttca
     4861 aatggtgacc ttgaccggag tcgaggtcgc tggtgacatg gcgcatgcca aggtgtttta
     4921 tacagcacaa aaaaacagca aaggcttaca ggctggtctg gagaaggcgg ctggcttctt
     4981 gcgctcgcag ttgggtaagc gcatgatgat acgtaccgtg ccgcaattac attttgtcta
     5041 tgatgaatcc atagaccgtg gcatgcacat ggacaagctt atccatgacg ccttgtccga
     5101 tacccctccg caagaataat ttaaatccca tacagctatg caaaccaaac gcataaaacg
     5161 cgtcgttaac ggcgtgttgc tgctcgacaa accctatggc ttttcttcca accaggcttt
     5221 gcaaaaggtc aaatggttat atcaggcaca gaaagcaggt cataccggta cattggaccc
     5281 cctggcaacc ggcgtgttgc ccatttgttt tggggaggcg accaagtttg cccagcattt
     5341 aaccgacgaa aataaaacct atatcgccac ggtcaggttt ggtgtcatga cgacgacggg
     5401 tgataaagag ggcgatgtga tcagccaggt agattgccag gtgacgcgca aagatattga
     5461 gcaggttttg ccacagttta tgggtgaaat tacgcaggtg ccgcctatgt attctgcact
     5521 aaaagtgaac ggcaagccct tgtatgaata tgcacgtgat ggcgtagagc tggagcgtgc
     5581 accccgccag gtcaccattc atgccttacg cgtgctcagt tgcgctgaca atcatgccga
     5641 aatcgaagtc tgctgcagca aaggcaccta tatccgcact ctgggtgaag atatcggtaa
     5701 agcattaaac gttggtgcgc atttgacggc tttgcggcgt acggcaacgg ccacctatca
     5761 aattgcgcag acggtaacga tagaagcgct ggagcagatg gcaatcgagc aaaaggatgc
     5821 attgttgtta cccgtggaca cggcgattac ggcgctgccg aaaatagaac tgcatgagga
     5881 tgcggcctat tatttcaagc aaggcaatcc cgtatgggta tccggcaata ttccagcggg
     5941 tgagttacgt atttatagtg aaaccggcct atttcttggt gtcggtcaac aacagcgtga
     6001 tggccgtatt gcgcctaaac gtgtcgtcaa tctctaaccg ttcatgttgg gctctgcttg
     6061 ccagtgccgc agcaagtttc tacaatcagc ggacgctcta atctgatagg tgtactcatg
     6121 agaatacctg tttcccaagc gttattgatg acgctcctgc tgccccttac ggtgactccg
     6181 gcagaggaaa gcgacacaag gtctagcgga aaccaacgtc cggcggcaat gaccatgctt
     6241 tatgatgctc gtttcgagtt acatgacccg ggccccggcc atcctgagcg ggcaagccgc
     6301 gtgcatgcga ttgtggacca gctaaaacgc aatgagtcac tgaatccctt tttacgctgg
     6361 cctgcttttt cagcagccac gcgggcagac ttgttgcgtg tacacagtcc agcctacctg
     6421 gacttgctgg ataaagagca agccagcgtt aagcctgggc agtatcactt actaagtacc
     6481 ggcgatacca tgctgtcaca acactcactg gccgttgcca cattggcttc tggtgctgtc
     6541 atggcggggg tcgatgccgt gatgacggcg cagtcgccag tggcctttgc tttggtcagg
     6601 ccacccggtc accatgccac ccgcgatcga ggcatgggct tttgcctgac caaccatatt
     6661 gcggtggcga ctcgctatgc ccagcacaaa tacggcatac agcgcatttt aattattgat
     6721 atcgacgtgc atcatggtaa tggtacgcag gatatttttt atgcagataa cagcgtgttt
     6781 tacttcagcg cgcatcagca tcccttatac ccgggcagtg gccggcccag tgaaaccggc
     6841 gtggggccgg ggctgggcta caccacgaat gtggatgttc cgcccggcag taatgaaagc
     6901 cgtatgttac aggcgattga gcggcagctg gtgccagcca tgcagcaatt ccgtcctgaa
     6961 ttgatcatgg tgtcggccgg gctcgatgca catgcgggag atactttagg cgcattacag
     7021 tattccgatg cgggctatgc cgcgcttgca acagcaacca aacaattggc agcacactac
     7081 acacaaggac gcatggtgtg gatgctggaa ggcggttatg tgccagcaaa taatgccaat
     7141 gctgtggagg cgatcactcg agtattgatt gcggtaccat agctgttcat tcgattag
//
LOCUS       DATMSK010000080         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_358543,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000080 DATMSK010000000
VERSION     DATMSK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358543"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..747
                     /gene="rsmG"
                     /locus_tag="VNZ84_02820"
     CDS             121..747
                     /gene="rsmG"
                     /locus_tag="VNZ84_02820"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HWU65914.1"
                     /translation="MDYLLSQLVAGVEAMGLQSTVTPEMQTQCLTYLALLQKWNKVYN
                     LTAVRDPQEMLILHLLDSLSVLPYIQAGHLLDVGSGGGLPGLVIAITRPDVQVTTIDT
                     VQKKTIFMRQVKAELGLSNVDVVHARVESYAPPSPFTQVISRAFSDIALFKQLTSHLM
                     APHGHWLAMKGLVPTEELAAAGVQPKEVIRLQIPNLQAERHLIVFENT"
     gene            747..1508
                     /locus_tag="VNZ84_02825"
     CDS             747..1508
                     /locus_tag="VNZ84_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU65915.1"
                     /translation="MKIIAITNQKGGVGKTTTCVNLAASLAEHGKKVLLVDLDPQGNA
                     STGSGIDKAHLKDSIYQVLIGNKTLQEVVIHSEAAKFDVAPSNRDLAGAEVELVNEMA
                     RETRLKNAILKLGGDAYDFVLLDCPPSLNLVTVNALTAAHSVMIPMQCEYYALEGLSD
                     LVNTIKKVRAHLNPVLEIEGLLRTMFDNRNMLAQQVSAQLVNHFGKKVYQTIIPRNVR
                     LAEAPSYGMPVLLYDRSSKGAQAYLELAQEIVQKN"
     gene            1554..>2376
                     /locus_tag="VNZ84_02830"
     CDS             1554..>2376
                     /locus_tag="VNZ84_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWU65916.1"
                     /translation="MVKPKGLGRGLDALLSGDTETTSESDTLRTLPVGQLQPGKYQPR
                     SYMNEDALQTLADSIAAQGIMQPILVREIGPEKFEIIAGERRWRASQKAGLTEVPVLV
                     REIADEAALAMALIENIQREDLNPLEEAMGIKRLIDEFDMTHEKAAEAVGRSRVAVSN
                     LLRLLTLTPMVQDMLLDNQIDMGHARALVGIEPAQQILLANKVVQDGLSVREIERIMQ
                     TEPASGKKAKPMVHPDVATLQTQLSEQLGAEVAISSKANGAGVLKLNFANLDQLDD"
BASE COUNT          650 a          511 c          640 g          575 t
ORIGIN      
        1 aacgattggc caggcgggcc gcatacaggg aatcacgccg gcgactattt aactgttgtt
       61 ggtatatctc aagcgtaagc agaaaaaaca aagccaataa ggcgatattg cggcggcata
      121 atggattatc tattgtcgca attggttgct ggggtagagg caatgggttt gcaatctacg
      181 gtcacaccgg aaatgcagac gcaatgtctg acctatcttg ccttattgca aaagtggaac
      241 aaggtttata acctgaccgc agtgcgtgac ccgcaagaaa tgctgatatt acatttgctg
      301 gatagtttaa gcgtgttgcc ttatattcag gcaggtcatt tgctggatgt ggggagtggt
      361 ggtggcttgc caggcttggt gattgccatt acccgtccgg atgtgcaagt cacaacaatt
      421 gataccgtgc agaaaaagac cattttcatg cgacaggtta aagcggagct tggattgtcg
      481 aatgtagacg ttgtgcatgc cagggtcgag agttatgctc cgccctcccc gtttacgcaa
      541 gtcatttcgc gcgctttttc ggatatcgct ttatttaagc aattgacctc gcatctgatg
      601 gctccacatg gtcactggct ggcgatgaag gggttggtgc caacggaaga gcttgcagct
      661 gcaggtgtgc agcccaaaga ggtcattcgt ttgcaaatac caaacttgca ggcggaaaga
      721 catttaattg tgtttgagaa tacataatga aaataatagc aatcaccaac caaaaagggg
      781 gcgtcggtaa aacgacaact tgtgtcaacc tggcagcaag cctggctgaa catggaaaaa
      841 aagtattgtt ggttgattta gatccgcagg ggaatgccag cacaggtagc gggattgata
      901 aggcacattt gaaagatagc atctatcagg tcctgatcgg taataagact ttgcaagagg
      961 tggtcattca tagtgaagcc gccaagttcg atgtggcgcc gtcaaatcgc gatcttgcgg
     1021 gggctgaggt ggagttggtg aatgaaatgg cacgcgaaac gcggcttaaa aatgccattc
     1081 tcaagttggg tggtgatgca tatgattttg tcttgctgga ctgccctccc tctcttaacc
     1141 tggtcacggt aaatgcactg acggcggcgc attcagtcat gattcccatg cagtgtgaat
     1201 actatgcgtt ggagggcttg tcagacctgg tgaataccat caagaaagtg cgcgctcact
     1261 taaatcctgt tcttgagata gaaggcttgt tgcgcaccat gtttgataat cgcaatatgc
     1321 tggctcaaca agtatcggca cagctggtca atcacttcgg taaaaaagtg tatcaaacca
     1381 ttattccacg caacgttaga ttggctgagg cacccagtta tggaatgccg gtgttgctgt
     1441 atgatcgctc atctaaaggc gcacaggcct acctcgagct ggcacaggaa atcgtccaga
     1501 aaaattaatc agacaaaaac aatttaaaga tactcaacaa aggaagcagc agcatggtga
     1561 agccaaaggg acttggacgc ggacttgacg cattgttatc gggggataca gaaaccactt
     1621 ccgagtcgga cacattgcgt accttgcctg tggggcagtt gcagccaggc aaataccagc
     1681 cccgctccta catgaatgaa gatgcgcttc aaacgctggc ggactcgatt gcggcacaag
     1741 ggatcatgca gcctatcctg gtccgtgaaa tcgggcctga gaaatttgaa attattgctg
     1801 gtgaacgccg ctggcgcgct tcacaaaaag ccgggctcac cgaagtgcct gtcctcgtcc
     1861 gtgaaattgc ggatgaggca gccttggcca tggcactgat tgaaaatatc cagcgtgaag
     1921 atttgaatcc gttggaagaa gcaatgggta ttaagcgact gattgacgaa tttgatatga
     1981 cgcatgaaaa agcggcagaa gccgttggcc gctcgcgcgt ggcggtatcc aatttactta
     2041 gattactcac gctaacgccc atggtgcaag atatgctact ggataaccag attgatatgg
     2101 gtcacgcccg tgccttggtg ggaattgagc cggcccaaca gattttactg gcaaataaag
     2161 tggtacaaga cggcctttcc gtacgcgaaa ttgaacgcat catgcaaacc gagcctgcca
     2221 gtggtaaaaa agccaagccg atggtacatc ctgatgtggc aacattacag actcaattaa
     2281 gtgaacaact gggtgcagag gttgccatta gtagtaaagc aaatggtgcg ggtgtgttga
     2341 agttgaattt tgcaaacctt gatcaattag atgatt
//
LOCUS       DATMSK010000081         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_124099,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000081 DATMSK010000000
VERSION     DATMSK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124099"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VNZ84_02835"
     CDS             <1..202
                     /locus_tag="VNZ84_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65917.1"
                     /translation="GTRSVDSIEFTAVKGYPKSNCHSCDNQSDYTFVSMTVIPVPEAH
                     TSAMMFAGLLMIGGLAARRMKS"
     gene            353..901
                     /locus_tag="VNZ84_02840"
     CDS             353..901
                     /locus_tag="VNZ84_02840"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HWU65918.1"
                     /translation="MLDRDGYRPNVGIVLCNAHNQVFWGKRIREHAWQFPQGGIKYGE
                     TPEQAMYRELMEEVGLKPEHVKILGRTRDWLRYEVPTSWIKREWRGSYKGQKQIWFLL
                     RLTGRDSDVSLRATEHPEFDAWRWSEYWVPLEDVIEFKREVYQLALNELATFLHHSGR
                     RHGGKPRKPAVTNKQPPTVTPE"
     gene            complement(908..1423)
                     /gene="msrA"
                     /locus_tag="VNZ84_02845"
     CDS             complement(908..1423)
                     /gene="msrA"
                     /locus_tag="VNZ84_02845"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWU65919.1"
                     /translation="MQDTIILAGGCFWGMQDLFRRLPGVISTRVGYTGGEVANATYRN
                     HGNHAEAIEITFDSASISLRQLLVFFFQIHDPTTLNRQGNDLGPSYRSAIYYANPAQK
                     ETAQQLMTEMDASRVWPGKIVTEVTPASDFWLAEPEHQDYLVKYPTGYTCHGLRPDWI
                     LPQDRASAVWL"
     gene            1587..2588
                     /gene="gap"
                     /locus_tag="VNZ84_02850"
     CDS             1587..2588
                     /gene="gap"
                     /locus_tag="VNZ84_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWU65920.1"
                     /translation="MSVKVGINGFGRIGRMALRAAIEQAEFSNIEVVAINSSYDVEYM
                     MYLLKYDSVHGRFNAKVEADNGALVVNGKRIHLTAERDPNNIDWRKGGAEVVIESTGA
                     FLTQETCQSHVNGGAVKVVQSAPGKDDTPMFVYGVNHTEYAGQAIISAASCTTNGLAP
                     LAKVLHDTFGIKRGLMTTIHAATASQLTVDGTSKKDWRGGRSVFENIIPSSTGAAKAV
                     GKVIPSLNKKLTGMSMRVPSADVSVVDLTVELNSEATYEAICAAMQQAADGALKGVLE
                     YTNDKVVSSDFRSSPAASVFDAGAGIMLDPTFVKVVGWYDNEYGYTCNLLRLVQYIA"
     gene            2683..>3468
                     /locus_tag="VNZ84_02855"
     CDS             2683..>3468
                     /locus_tag="VNZ84_02855"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWU65921.1"
                     /translation="MKFIKMTDLDLRGKRVFIRADLNVPVKDGVVTSDARIVASMPTI
                     EFALKAGAKVMVTSHLGRPEEGVYSEENSLQPVADVMSKLLGQPVRLVKNWLAPNAPE
                     DSLTTQDGQLILLENCRFNVGEKKNNDELAKKYAALCDVFVMDAFGTAHRAEASTHGI
                     AKFAPVACAGILLTEELDALSKALLAPARPLVAIVGGSKVSTKLTVLESLADKVDQLV
                     VGGGIANTFLKASGQEVGKSLCEDDLVDTAKSLMYKMAARGACV"
BASE COUNT          834 a          748 c          974 g          912 t
ORIGIN      
        1 tggaactcgc tctgttgata gtattgagtt tactgcggtt aaaggttatc caaagtctaa
       61 ctgccacagc tgtgataatc agtcggatta cacttttgtt agcatgaccg tgattcctgt
      121 tcctgaagcc cacacatctg ccatgatgtt tgccggcttg ttgatgattg gtggcctggc
      181 tgcgcgtcgc atgaaatcct gatctttatg gatcgctgta aaaagctcac gaaggtgagc
      241 tttttttttt gagtaaaacg cttgtacttt tatggtatgt gcatgatggt tgtcctttca
      301 ttaaaaggca aagtgtgaaa aaattgcagc aaatattgaa ggcagttttt ttatgttaga
      361 tcgtgatgga taccgtccaa acgtcgggat tgtgttatgc aatgcacata atcaggtgtt
      421 ctggggcaag cgtatcaggg agcatgcatg gcagtttccg caaggcggca tcaagtatgg
      481 tgaaacgcct gagcaggcca tgtatcgtga gctgatggag gaggtgggcc tcaagcctga
      541 acacgtgaaa attctgggcc gcacgcgtga ctggttacgc tatgaggtcc cgaccagctg
      601 gatcaagcgt gagtggcgtg gcagttacaa gggccaaaaa caaatctggt ttttattgcg
      661 tctgacgggg cgtgattcag atgtttcgct gcgtgcgacc gagcatcctg agtttgatgc
      721 ctggcgctgg agcgaatatt gggtgccatt ggaggatgtg attgagttca agcgcgaggt
      781 atatcaacta gcgctcaacg agctggcaac atttttacat cactccggcc gccgtcatgg
      841 cggcaaacca cgtaaacctg ctgttacgaa caagcagccg ccaactgtga cgccagagta
      901 gccttagtta caaccaaact gcagatgcac ggtcttgcgg caagatccag tctggccgta
      961 gtccatggca ggtgtagcca gtcgggtatt tgactaaata gtcctgatgc tctggttcgg
     1021 caagccagaa gtcactggcg ggagtgactt ctgtgacaat cttccccggc caaactctag
     1081 aagcgtccat ttcagtcatt agttgctgcg cagtttcttt ttgtgctgga ttcgcatagt
     1141 aaattgcaga gcggtatgac ggacccaaat cattcccctg gcggttcagc gtggttgggt
     1201 cgtgaatctg aaaaaagaac accagtaact gcctgaggct gatgcttgca gaatcaaacg
     1261 taatctctat cgcctcagca tgattgccgt gattgcggta agtggcattg gctacttcgc
     1321 cgccagtata gccaacacgt gtgctgatca cccctggcaa tcgacggaat aaatcctgca
     1381 tgccccagaa gcagccgcca gcgagaataa tggtgtcttg cataaggtcc tctttctatt
     1441 aatcagatgc ttattgtcag catgatgttg tgtttgaatg actttcttgc tttgtcgtgt
     1501 atcaaatcaa acacttacaa tgtgtccagt ataggtggtt taggttaaaa tgctgagttt
     1561 tagttctaag caatgttggg gagcatatgt cagtcaaggt aggcattaac gggtttggac
     1621 gaattggccg tatggcgtta cgtgcggcga ttgagcaagc agagttcagc aatatcgaag
     1681 tggtggcaat taatagctca tatgatgttg aatacatgat gtatttgctc aagtatgact
     1741 ctgtgcatgg ccgttttaat gccaaggtgg aagctgacaa tggcgctctg gtggtgaatg
     1801 gcaaacgcat tcacctgact gcagagcgtg atcctaacaa tattgactgg cgcaaaggtg
     1861 gtgctgaggt ggtgattgaa tctaccggtg cttttttgac gcaggaaacc tgtcagtcgc
     1921 acgtgaatgg cggcgcggtc aaagtcgtac agtctgcacc aggtaaagat gatacgccga
     1981 tgtttgtgta cggcgtaaac catactgagt acgctggcca ggcgattatc tccgcagcgt
     2041 cttgcacgac taacggactg gcacctttag caaaagtact gcatgatacg tttggtatca
     2101 aacgcggctt gatgacgacg attcacgcgg ctacagcgtc ccaattaacc gtagacggga
     2161 cctcaaaaaa agactggcgc ggtggccgca gcgtgtttga aaatattatt ccttccagca
     2221 cgggtgctgc caaagccgtt ggcaaggtta ttccatcctt gaacaaaaag ctgacaggga
     2281 tgtccatgcg cgttccttct gcagatgttt ccgtggttga tctgacggtt gagctcaata
     2341 gcgaagcgac ttatgaagcg atttgtgcgg ccatgcaaca agcagcagac ggcgcgctga
     2401 aaggtgtact tgagtacact aatgataaag tggtttccag tgattttcgc agtagtcctg
     2461 cggccagcgt gtttgatgcc ggtgctggta ttatgctgga cccgacgttt gtgaaagtgg
     2521 tgggctggta cgataacgaa tacggttata cctgtaacct gctgcgcctt gttcaatata
     2581 ttgcttaatc atgcacgggg ctttatgccc cgtttgcata tcacggattg ataccttacc
     2641 agtttatatt caagcggcgg tgaccgctta agggagacct tcatgaaatt catcaaaatg
     2701 accgacctcg atttgcgtgg caagcgtgtg tttatccgag ctgacctcaa tgtaccggta
     2761 aaagatggtg tagtgaccag tgatgccagg attgtagcga gcatgcctac cattgagttt
     2821 gccttgaaag caggcgccaa agtgatggtg acttctcatt tgggtcgccc ggaagagggc
     2881 gtttacagtg aagagaactc attgcagccg gtggctgatg tgatgagcaa gttgctgggg
     2941 cagccggtac gcctggtgaa aaactggctg gcacctaatg caccagaaga cagtttaacc
     3001 acgcaagatg gccagctgat tttgctggaa aattgccgct ttaatgtggg ggaaaagaaa
     3061 aataacgatg agctggcgaa aaaatatgct gccttatgcg atgtgtttgt catggatgct
     3121 tttggcacgg cacatcgcgc cgaagcctct acgcatggga tcgccaagtt tgcacctgtg
     3181 gcctgtgccg gcatcctgct gacggaagag ctggatgcct tgagtaaggc cttgctggca
     3241 cctgcacggc cactggtggc gattgttggt ggctccaagg tctcaaccaa attgaccgtg
     3301 ttggagagtt tggcggacaa ggtggatcag ctggtggtgg gtggcggtat cgcgaatacc
     3361 tttcttaagg cttctgggca agaagtaggt aagtctttat gtgaggatga cttggttgac
     3421 accgcgaagt ccctgatgta taaaatggcc gcgcgtggcg cctgtgtg
//
LOCUS       DATMSK010000082        10388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_147445,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000082 DATMSK010000000
VERSION     DATMSK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147445"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /gene="flgK"
                     /locus_tag="VNZ84_02860"
     CDS             complement(<1..1598)
                     /gene="flgK"
                     /locus_tag="VNZ84_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HWU65922.1"
                     /translation="MSNVLTIGKSALNAAQVGIATVGHNIANASTPGYSRQVVVQAAA
                     QAQNFGYGFVGQGANVVGIQRVYNETLAKQVINATSMAKASSAYYSNISQINNLLSDS
                     TAGLNPVITSFFSAVSAAAANPSDTATRQTFMSSAQSMINRFNSVNNQLQDMRNSINT
                     QITASVDTINSYASQIATLNDTINKALNSTGNPPNDLMDQRDQLVAKISEQIQTTVIK
                     QDDGSYNVFAGSGMPLVVGTTQYSLYTRASETDLGRVEVAYGNPDSPKILGNETLSGG
                     TLGGVLKFRSDTLDVVQNQIGQLALTLTSQFNAQQSQGLDLNGIAGTNLFNTIQPQVT
                     PSSRNPSTSPNITASFDNTSMVTSSDYSVRYTSGNYVITRLSDNTLMPEGSTGGVTLP
                     QTFNGITIDAGAGTPAEGDYYLVQPTRGAASNLSLNFTDVSKLALADATGGPGNNVNG
                     LALAKLQTGYLDDGTTTLTQGTVRLGGTDANPVYGTYSRSFSQMVSAVGTKTNELSIT
                     SVADATALDNATSAMQSESGVNLD"
     gene            complement(1706..2776)
                     /gene="flgJ"
                     /locus_tag="VNZ84_02865"
     CDS             complement(1706..2776)
                     /gene="flgJ"
                     /locus_tag="VNZ84_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HWU65923.1"
                     /translation="MQPRVDPTAQGLAIDGNSLNSLKRANNDSPEAIRAVARQFEAVL
                     MNMMLKSMRDTVPQDGITNSEQGKMFTSMLDQQLSTHLSQKGMGLTDVLVRQLSKFSQ
                     KPADATDGKDAKPTGKVDSTLAPANTSSNTSNGKAVLAQAVQKIREAYQQWEATDSDN
                     VGKNSETDWAQGIPELPPLVAETQENVQSFTLPQAPLATAMREVAGKTQQFIQSMLPH
                     AQAASNVSGIPAKFMIGQAALESGWGKHEIKTDNGVNSHNLFGIKADANWKGKVATST
                     TTEYINGVKQTRVEKFRAYDSYSDAFKDYAKLISQNPRYQQAMNNTHDASAYAHALQR
                     AGYATDPQYGKKLTQVIKNLQG"
     gene            complement(2790..3926)
                     /locus_tag="VNZ84_02870"
     CDS             complement(2790..3926)
                     /locus_tag="VNZ84_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HWU65924.1"
                     /translation="MPTLTHLFSSSQYCRRAIALLAISLMLVTPFAQAERIKDISNIQ
                     GVRANQLIGYGLVVGLDGTGDMTTQTPFTVQSIISMMNQLGITIPPGTSLQLKNVAAV
                     MVTSSLPAFAQPGQTLDVTVSSMGNAKSLRGGTLLMTPLKGADGQIYAMAQGNLVVGG
                     VGAAANGSQTQINHLSVGRISEGATVERALPNTLLHQDIVKLELKESDFSTANNVVEA
                     INKKFGPMTAQAESGRVIQVRPPESMSRVSFLANLETINVALAATPAKIILNARTGSV
                     VMNKAVTLDSCAVSHGNLSVVINTSPVISQPGPFSNGQTISTAVSEINIKKEPGKVLK
                     LNNGANLSDVVKALNAIGTTPQDLLAILQAMKAAGALNAELEVI"
     gene            complement(3938..4588)
                     /locus_tag="VNZ84_02875"
     CDS             complement(3938..4588)
                     /locus_tag="VNZ84_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HWU65925.1"
                     /translation="MSLLLCLALSQLLNGCSITPSTIVQKPDTPRVAMARPDARTEGA
                     IFNSAAFRPLFEDRRPRYVGDIVTIRITENTTANKASDNEQTKDGSTATSVTGLFGHN
                     VPKAVFNASSALSFKDKATADASNTFSGSVTATVTDVLPNGYLVVSGEKQVGFDKGTE
                     YVRFSGVVNPDMITLGNEVLSTKVADARIEYRSNTKMDAAEVLSMFSRFFLSMIPL"
     gene            complement(4628..5410)
                     /gene="flgG"
                     /locus_tag="VNZ84_02880"
     CDS             complement(4628..5410)
                     /gene="flgG"
                     /locus_tag="VNZ84_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HWU65926.1"
                     /translation="MIRSLWISKTGLDAQQMQMDVISNNLANVSTSGFKKSRAVFEDL
                     LYQNIRQVGSQSSQQSQLPSGLQLGTGVKPVATERIFTQGNLQQTSNDKDIAIQGQGF
                     FQILLPDGTTAYTRDGSFQVDSQGQLVTASGFPVQPSITVPANAQSLSVGRDGVVSIT
                     TPNSTATTQIGQIQLATFINPAGLQAKGENLYVETTASGNPNTNTPGTNGAGLLSQGY
                     VETSNVNVVEELVSMIQTQRAYEINSKAITTSDQMLQKLSQM"
     gene            complement(5464..6207)
                     /gene="flgF"
                     /locus_tag="VNZ84_02885"
     CDS             complement(5464..6207)
                     /gene="flgF"
                     /locus_tag="VNZ84_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HWU65927.1"
                     /translation="MDRLIYTAMTGAKHILEQQATTAHNLANATTTGFKAQVDSFRAV
                     PILGDGLPTRAFVVDATVGTDYKAGSIQHTGRDLDVAIQGQGWIAVQRADGSEGYTRN
                     GSLKINENGQLQTESGLNVMGDGGPISIPPNVIVSIGSDGTISSVNDFTAPGATTILG
                     RIKLANPPEASLKRSEDGLFVTSNGQAVPLDPTVKVTGGALESSNVNVVETMVNMISL
                     ARQFEMQMKMLDNAQNNAGKADQLFSMNG"
     gene            complement(6244..7455)
                     /gene="flgE"
                     /locus_tag="VNZ84_02890"
     CDS             complement(6244..7455)
                     /gene="flgE"
                     /locus_tag="VNZ84_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HWU65928.1"
                     /translation="MAFSQGLSGLNAASKALEVIGNNVSNANTVGFKQSRAEFADVFA
                     ASLSGAGATQIGIGTKISDVAQQFTQGNITATNNTLDVAINGAGFFRLSNNGAVTYTR
                     NGQFQMDKNGYIVTSDGKRLMGNSADNLNGSSPVEIQIDTSDLQPVASTKITGTLNLN
                     SASANIDQTVTPFSSTDPTSYTNSTAITVYDNLGNSHNVQNYYVKTAANTWSVYTTVD
                     GTDVTPTPTPSMVFNAGSYDSANSSVTPISFTPTTGGATMNISFDYSKSTQYGSSFSV
                     NKLSQDGYSSGRIASFTIGDDGIIQGRYTNGQSKDLGRVILANFVNPNGLQSLGGNQW
                     MASYESGDELIGAPGSSTLGVLQSSAVEDSNVDLTAELVNMITAQRVYQANAQTIKTQ
                     DQVMQTLVNLR"
     gene            complement(7496..8164)
                     /locus_tag="VNZ84_02895"
     CDS             complement(7496..8164)
                     /locus_tag="VNZ84_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HWU65929.1"
                     /translation="MTTVNTNNQVSQDLLNSVNTRSSASTTDETQDRFMTLLVTQMKN
                     QDPLNPMDNAQVTSQMAQLNTVTGINKLNETMSSLIGSVQLGQSYQATSMIGRNVLVE
                     GKTLTHTETGGYFGVNVPNGADSLSVAIKNGSGATIRTLTFGKQDAGVNALSWDGKMD
                     DGTVAPSGDYTYEITAKTGDTTVSSTALSLAQVQSVTTSTSGAKLNLSNNTSVAIADI
                     QEIF"
     gene            complement(8185..8589)
                     /gene="flgC"
                     /locus_tag="VNZ84_02900"
     CDS             complement(8185..8589)
                     /gene="flgC"
                     /locus_tag="VNZ84_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HWU65930.1"
                     /translation="MSLFNVFKISGSAMSAQSMRLNTVASNLANADSVVSSDGKPYQS
                     KQVVFKAVQDAANQSSTVEVDRVIEDTKPGKMVYDPKHPMANADGYITMPNVNVTEEM
                     VNMISASRAYQNNVETMNAAKTMLLKTLNIGQ"
     gene            complement(8606..9040)
                     /gene="flgB"
                     /locus_tag="VNZ84_02905"
     CDS             complement(8606..9040)
                     /gene="flgB"
                     /locus_tag="VNZ84_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HWU65931.1"
                     /translation="MMNKLDAALNFHQTALRVRNQRQELLASNIANADTPQYKARDID
                     FKSAMQSALQQTTTGNTTANAGAMQQTNAGHMTGQASNGYVGSNSDPLFRSVVQGSVD
                     GNTVDMDVERNAYVDNGIRYEASLTMIGGQIKKMLSAINGQQ"
     gene            9277..10032
                     /gene="flgA"
                     /locus_tag="VNZ84_02910"
     CDS             9277..10032
                     /gene="flgA"
                     /locus_tag="VNZ84_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HWU65932.1"
                     /translation="MKRLPIHIAMSLFVSLLWLMSASVRAADQAVIANQRPAATAAGD
                     MHSRVYQTVLQYVQSQTQGYPGKVNIEIQPLDSRIRIAECGLMEGFTVQGARLWGKTH
                     IGVRCLQSETKPWTLYVQADVQVWGEYAVTGLPVSQGSPVQTTDVVMQSGDLSKLPAG
                     IITDLNMLAGKQAALNLPLGTVLRPELLKAVPVIMQGQTVQLNSRGEGFVITADGTAM
                     QTANVGQVVDVKVMSGQIVKGIAQASGKVDVRF"
     gene            10202..>10388
                     /locus_tag="VNZ84_02915"
     CDS             10202..>10388
                     /locus_tag="VNZ84_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HWU65933.1"
                     /translation="MNISDSIKKMGVGVDKPTLEKGAANKSSEKAASTSTASSDNVTL
                     SSASVQLQSLEAGLASGE"
BASE COUNT         2277 a         2504 c         2716 g         2891 t
ORIGIN      
        1 tcgtctaaat tgacccctga ttcagactgc atggcgctag tcgcgttatc aagtgcagtc
       61 gcatctgcaa cgctagtgat acttagctca ttagtttttg tgccaactgc actcaccatt
      121 tgagagaatg accgcgagta agtaccgtat actggattag catcagtacc tcccaacctg
      181 accgtacctt gggtaagggt ggtggttcca tcatccagat agccggtttg tagttttgct
      241 aaagccagac cattcacatt attgcctgga ccacccgtgg catcggcgag cgcgagttta
      301 ctcacatccg tgaaatttaa agaaagatta cttgcagcgc cccgtgtcgg ctgaacaaga
      361 taatagtcgc cttctgccgg agtacctgca ccagcatcaa tcgtgatgcc attgaatgtt
      421 tgtggtaggg ttactccacc tgtactgcct tctggcatca gcgtgttatc actgaggcgt
      481 gtaatcacat agtttcctga agtatatctc acgctgtaat cactgcttgt caccatgctg
      541 gtgttgtcaa aagaagcggt gatattgggt gaagtactcg gattcctgct gcttggcgtc
      601 acttgcggtt gaatggtgtt aaaaagattg gtgcctgcaa tgccgttcag gtccagccct
      661 tggctttgtt gcgcattgaa ttggctggtt aaagtcaaag cgagttgccc aatctggttc
      721 tgtacaacat ccagcgtgtc acttctaaat ttaagcacgc cgccaagcgt accgccgcta
      781 agcgtttcat ttccaaggat ttttgggcta tcaggattgc cgtaagccac ttctactcgg
      841 ccgagatcag tctcgctcgc tctggtatac aaggagtatt gtgtcgtgcc gaccacgagt
      901 ggcatgccac tgccggcaaa tacgttgtaa gaaccatcat cctgtttgat aacggtcgtt
      961 tgaatttgct cactgatttt agcgacaagc tggtcacgtt gatccatcag gtcgtttggt
     1021 gggttgcctg tagagttaag cgccttgtta atggtgtcgt tcagggttgc gatttgggag
     1081 gcgtagctgt tgatagtgtc tacgcttgct gtgatctgcg tattaatact gttacgcata
     1141 tcctgaagtt ggttattcac cgagttgaag cggttgatca tggattgcgc cgaagacatg
     1201 aatgtctggc gcgtggccgt atcactcggg tttgcagcgg cggcactcac cgcgctaaaa
     1261 aagctggtga tcacagggtt taagccggca gtactgtctg acagcaggtt gttaatctgg
     1321 ctgatgttgc tgtaataggc actactggct ttggccatag aggtggcatt aatcacctgt
     1381 ttggccagag tctcattgta aacacgctgt ataccgacga cattcgcacc ctggccaaca
     1441 aagccataac cgaagttttg cgcttgcgct gcggcctgta ccacgacttg acggctataa
     1501 cccggtgtcg aggcgttggc aatgttatgg ccaacggtgg caatgcctac ttgtgcggcg
     1561 ttgagtgcgc tcttaccaat ggtgagtaca tttgacatgt tttttcctca tgtgccagcc
     1621 ccctgcagag agtcagacac aactttaaag tgatggttaa gtagcctatc ggcagttttt
     1681 tttgtttctt tagggtcgga cttgcttagc cttgtaaatt cttaatgact tgcgtcagtt
     1741 tcttgccata ttgcgggtca gtagcatagc ccgcgcgttg taaggcatgg gcatacgcgc
     1801 tggcatcatg ggtgttattc atggcttgct ggtagcgcgg attttgcgaa atcagtttgg
     1861 cgtaatcctt gaaagcatcg ctgtagctat cgtaagcgcg gaatttttca acccgtgttt
     1921 gcttcacgcc attgatatat tcggtggtgg tgctggtagc gactttacct ttccagttgg
     1981 catccgcctt gatcccaaac aggttgtggc tattcacgcc attgtcggtt ttgatttcat
     2041 gtttgcccca accactttcg agtgcggcct ggccgatcat gaatttggcc ggaatgcctg
     2101 acacattgct ggcagcctgg gcatgcggca acatactctg gataaactgt tgtgttttcc
     2161 cggctacttc acgcatggcg gttgccaatg gtgcctgggg cagggtaaag ctttgcacat
     2221 tttcctgggt ttctgcgact agcggcggta attcagggat gccctgtgcc cagtctgttt
     2281 cactgttttt acctacattg tcgctatcag tcgcttccca ttgctgataa gcctcgcgta
     2341 tcttttggac cgcctgtgcc aacaccgctt tgccgtttga tgtgtttgag ctggtattag
     2401 ccggtgcaag cgtactatct actttgcccg ttggcttggc atctttgcca tcggttgcat
     2461 ctgcgggttt ctggctgaat ttactgagtt gcctgaccag tacatctgtg agtcccatgc
     2521 ctttttggct caagtgggtg ctcagttgct gatccagcat gctggtgaac attttgcctt
     2581 gttcactatt ggtgatgccg tcttgtggca cagtatcgcg catgcttttg agcatcatgt
     2641 tcatcagcac tgcctcaaac tggcgagcga cggcacgtat cgcctctggc gagtcattat
     2701 tggcacgttt caggctatta agcgagtttc catcaatcgc caggccttgt gcagtagggt
     2761 cgacacgagg ttgcatcact gtctctcgct taaattacct caagctcggc attcaatgcg
     2821 cctgccgctt tcatggcttg cagaattgcc agcaggtctt gtggtgtagt accgattgca
     2881 ttcaacgctt tcactacatc cgacaagttg gcaccattgt taagcttaag taccttgcct
     2941 ggttcttttt taatattgat ttcagagact gcggtagaaa tcgtctgtcc attggagaag
     3001 ggcccaggct ggctaatcac aggtgaggtg ttaatgacca cagacaagtt gccatgagac
     3061 actgcacaac tatccagagt gaccgccttg ttcatgacca cagaaccggt acgtgcattt
     3121 aggataattt ttgccggggt cgctgccagc gcaacattaa tcgtttccag attggccagg
     3181 aacgagacgc ggctcatgct ttccggtgga cgtacctgga tcacacggcc actctcagct
     3241 tgcgcggtca tcgggccaaa ctttttgtta atggcctcca ccacattatt ggcggtggag
     3301 aagtcgcttt ctttcaattc gagtttgaca atgtcctggt gtagcagggt attcggcagg
     3361 gcacgctcaa cggtcgcacc ttcggaaatc cggcctacac tcaaatgatt gatctgggtc
     3421 tggctaccgt tggccgcagc acccacaccg ccaacgacca ggttgccctg tgccatggca
     3481 taaatttgac catctgcccc ttttaaaggc gtcatgagca aggtgccacc acgcaaactc
     3541 ttggcgttac ccatggaaga tacggtcaca tccagggtct ggccaggttg cgcaaaagca
     3601 ggtaatgagc tggttaccat gactgcagcg acgtttttaa gttgcaggct ggtcccaggc
     3661 gggatggtaa tccccaattg gttcatcatg ctgatgatac tttgcacggt gaacggggtt
     3721 tgtgtggtca tgtcgcctgt accatctagg ccaacgacca agccgtaacc aatcagctgg
     3781 ttggcacgga cgccctggat attgctgata tccttgatgc gctcggcctg ggcaaagggt
     3841 gtgaccagca tgaggctgat tgccagcagg gcaatggcgc gtctgcagta ttgtgagcta
     3901 ctaaataaat gtgtcagggt tggcatgatc aactctccta taatggaatc atgctcagga
     3961 agaagcgtga aaacatggat aacacttccg cagcatccat tttggtattg ctgcgatatt
     4021 cgatacgggc atccgccacc ttggtggaga gcacttcatt gcccagggta atcatgtccg
     4081 ggttaacaac cccggagaac ctgacgtact cggtgccttt atcaaagccc acttgtttct
     4141 cgccactgac aacgagatag ccattgggca atacatccgt tacagttgca gtgactgagc
     4201 cactaaaggt gttgctggca tccgcggtgg ccttgtcttt aaaggacagg gcggagctgg
     4261 cattaaatac agccttaggt acgttatgcc caaacaagcc ggtcacagaa gtggcggttg
     4321 agccatcttt ggtttgctcg ttgtctgaag ccttgttagc agtggtgttc tcagtaatgc
     4381 ggatggttac gatatcgccg acataacgtg gacggcggtc ttcaaataat ggacggaacg
     4441 cggcactatt gaagatggct ccctcggtcc gtgcatccgg acgggccatc gcaacgcgcg
     4501 gcgtgtccgg tttttgtacg attgttgaag gtgtaatgga gcagccattc aataactgag
     4561 acaatgccaa acaaagtaat aagctcatga tcgttctatt catcatttga gtgaccctca
     4621 acagggatta catctgcgac agtttttgca acatctggtc agacgttgta atcgccttac
     4681 tattaatttc ataggcacgc tgggtttgga tcatactgac caactcttcc accacattga
     4741 cgttggaagt ctcaacatag ccttgtgata acaaaccggc accattggtc cctggcgtat
     4801 tggtgtttgg gttgccggaa gctgtggttt ctacatacag attttcacct ttggcctgca
     4861 agccagccgg attaataaaa gttgcaagct ggatctggcc gatttgtgtg gtggcggtag
     4921 agtttggcgt cgtgatagac accacgccgt ccctgccaac agacaagctt tgtgcgtttg
     4981 ccggaaccgt gattgacggt tgtaccggga agccacttgc cgtgactagc tggccctggc
     5041 tatccacctg gaatgaaccg tcgcgtgtat acgctgtggt gccgtctggt aacaggatct
     5101 ggaaaaatcc ctggccctgg atggcgatat ctttatcgtt actggtttgc tgtaagttcc
     5161 cctgagtgaa aatacgctcg gttgccactg gtttgacacc tgtgcccagt tgcaagccac
     5221 taggtaactg gctttgctgt gagctttgcg aaccgacctg gcgaatgttc tggtagagca
     5281 ggtcttcaaa caccgcgcgt gattttttaa agccgctggt actgacgttg gccaggttgt
     5341 ttgaaatcac gtccatttgc atttgttgtg catcaagacc tgttttggaa atccataatg
     5401 agcgtatcat tgttcactcc tttgggttaa acccgtgttc atattccaat attagtgcaa
     5461 acgctagcca ttcatactga agagctggtc tgcttttcct gcgttgtttt gcgcgttatc
     5521 cagcattttc atctgcattt cgaattgtcg cgccaggctg atcatgttga ccatggtttc
     5581 caccacgttg acattgctgc tttcgagtgc gccacccgtg actttgacag tcggatccaa
     5641 aggcacagcc tgaccgttgc tggtgacaaa taatccatcc tcagagcgtt taagtgaagc
     5701 ttctggtgga ttcgccagtt tgatccggcc caagatggtg gtcgctccgg gcgctgtaaa
     5761 gtcattgacg ctagaaatag tgccgtcgct accgatgctg acaatgacgt taggtgggat
     5821 gctgatgggg ccgccatcgc ccatcacatt taagccggat tcggtttgta actggccatt
     5881 ctcattaatc ttgagcgaac cattgcgtgt atagccttcg gagccatctg cacgttggac
     5941 ggcaatccag ccctggccct ggatggcgac atccaggtca cggccagtat gctgtatgct
     6001 gccggctttg tagtcggtac cgacggtggc atccacgaca aacgcacgcg tcggcaaacc
     6061 atctccgagg atcggcacgg cgcggaaact gtccacctgc gctttaaagc cggtggtggt
     6121 tgcgttggct aggttatggg cagtggtcgc ctgttgctcc agaatgtgct tggcgccagt
     6181 cattgcggtg tatatcagac gatccataaa gctgccccta tcaagaagtt aatcaaactt
     6241 tcattaacgc aggttgacca aagtctgcat gacctgatcc tgcgttttaa tggtttgtgc
     6301 gttggcctgg tagacacgtt gtgccgtaat catgttcacc aattctgccg tcaggtcgac
     6361 gttagagtct tcaacagcgg acgattgcaa tacgcccaat gtgctgctgc ctggcgcacc
     6421 aatcagttcg tcacctgact catagctcgc catccactgg ttgcctccaa gagattgcaa
     6481 accgttaggg ttcacaaagt tggccaggat cacgcggccg aggtctttgg attgaccgtt
     6541 ggtatagcgg ccttgaataa tgccgtcatc cccaatcgtg aagctagcaa tacggccaga
     6601 actgtaacca tcctggctca gcttgttgac gctgaaagaa gagccatact gtgtgctttt
     6661 cgagtaatca aaactgatgt tcatggtggc gccaccggtc gtcggtgtaa agctgattgg
     6721 tgtcacgctg gagttggctg aatcatagct acccgcatta aataccatgg atggtgtagg
     6781 cgtaggcgtc acatccgtac catcaacggt ggtgtaaaca ctccaggtat tggctgcagt
     6841 tttcacgtag tagttttgta cattgtgtga gttacccagg ttgtcataca ccgtgattgc
     6901 ggtggagttg gtataactgg ttggatccgt tgagctgaat ggtgtcactg tctggtcaat
     6961 attggcactg gctgaattca ggttcagggt gccagtaatt ttggtactgg ccacaggttg
     7021 caggtcactc gtgtcaatct ggatttctac cgggctggaa ccgttcaggt tgtctgcgct
     7081 gttacccatc aggcgtttac catcactggt cacaatgtaa ccgttcttat ccatctggaa
     7141 ttggccgtta cgggtgtaag tcaccgcgcc attgttgctc aggcggaaaa agcctgcgcc
     7201 gttaatcgct acatctagcg tgttgttggt cgcagtgatg ttgccctggg taaactgttg
     7261 ggccacatcg gaaattttgg taccaatacc gatttgtgtt gcacccgcac cactcagtga
     7321 agccgcgaac acatcggcaa attccgcacg agactgttta aaaccaactg tgttggcgtt
     7381 ggaaacgttg ttaccaatca cttccagcgc tttggacgct gcgtttaaac cactcaagcc
     7441 ttgtgaaaaa gccatgttat ctctccttgg taaagactga aatctaagtt gggaattaga
     7501 aaatctcttg aatatctgca atagccaccg aagtgttatt gctcaaattc agtttggcgc
     7561 cactggtgct ggtggtgacg ctttgtacct gtgccaggct cagtgcggtg ctgctcactg
     7621 tcgtgtcgcc cgttttggca gtaatttcat aggtgtagtc gccacttggc gcaaccgttc
     7681 catcatccat tttgccgtcc cagctcaggg cgtttacacc cgcatcctgt ttgccaaatg
     7741 tgagcgtacg gatagtggcg ccagagccgt ttttaatcgc cactgacaag ctatctgcgc
     7801 catttggcac attgacaccg aaataaccgc cggtttctgt atgcgtcagc gttttgcctt
     7861 ctaccaagac attgcgtcct atcatgctgg ttgcttgata ggactgcccc agttgtacgc
     7921 tgccaatcag gctgctcatg gtttcattga gcttgttgat ccctgtcaca gtattcaact
     7981 gcgccatctg gctggttacc tgggcgttgt ccatcgggtt taatgggtcc tggtttttca
     8041 tctgcgtcac cagcagggtc atgaaacgat cctgtgtttc atcagtggtg ctggcgctgc
     8101 tgcgtgtatt gacagagttg agcaggtcct gggaaacctg gttgttggtg ttaacggtgg
     8161 tcatgctgaa gtcctttctg caggttattg accgatattc agtgttttaa gcaacatggt
     8221 tttggcagcg ttcatggttt ccacgttgtt ttggtaggca cgggaagccg agatcatgtt
     8281 gaccatttcc tcagtcacgt tgacgttagg catcgtgatg taaccgtctg cgttcgccat
     8341 gggatgtttc gggtcataga ccattttgcc cggcttggta tcttcaatga cacgatccac
     8401 ctcgacagtg ctgctttgat tagcggcatc ctgtaccgcc ttgaacacta cttgttttga
     8461 ctggtaaggc ttgccatcgg agctgacgac gctatccgcg ttggcgaggt tactggccac
     8521 cgtgttcagg cgcatggact gcgcactcat ggcagagccg gaaattttga atacgttaaa
     8581 cagagacatg acagacctcc tttaattact gctgaccgtt aatcgcggac aacatctttt
     8641 taatctggcc accgatcatg gtcagactgg cttcatagcg gataccgtta tcgacgtacg
     8701 cattacgctc tacgtccata tctaccgtgt tgccatcgac agaaccttgc accacagaac
     8761 ggaacaatgg atcgctgtta ctgccgacat agccattgct tgcctggcca gtcatgtggc
     8821 ctgcgtttgt ttgctgcatc gcaccagcat tggcggtggt attgcctgtg gttgtttgtt
     8881 gcaacgcaga ttgcatggca gatttaaagt caatgtcacg tgccttatat tgtggcgtat
     8941 ctgcgttggc aatattggat gccaacaatt cctgacgctg gttgcgcacg cgcaatgccg
     9001 tctgatgaaa attcagtgcg gcgtcgagtt tgttcatcat cgtcttgtcc tttcatggct
     9061 gttttttcaa gccttgtccg aagcgtttgt tcgaacccct aaagttttgc cacttgcgac
     9121 cgttaacccg aataagcagg tctggtttta cacgtgtgac ttttactagc tatgcctagt
     9181 gtacgccgca caccgcacaa tgattcttga attaaacgtg ggaaatccct tttatttccg
     9241 ggattaagct tgccctgaat gcgtagaata gggcacatga aacgactccc cattcacatt
     9301 gcgatgtcac tctttgttag cttgttatgg ctgatgagtg cgtctgtgcg ggcggcagat
     9361 caagccgtga tagcgaatca gcgcccagca gcaactgctg cgggtgatat gcatagccgt
     9421 gtataccaaa cggtattgca atacgtgcaa agccagacac aagggtatcc aggcaaggtc
     9481 aatatcgaga tccagccttt ggatagtcgc atccgcatcg cagaatgtgg attgatggaa
     9541 ggatttactg ttcagggcgc caggctgtgg ggcaaaaccc atattggcgt gcgctgcctt
     9601 caatctgaaa ccaaaccatg gacactctat gtgcaggctg atgtgcaggt gtggggcgaa
     9661 tatgccgtga ccggcctgcc tgtcagccag ggttcaccgg ttcaaacaac agatgtggtc
     9721 atgcagtcgg gggatttgag caagttgcca gcgggcatta ttactgactt gaatatgctg
     9781 gctggtaaac aggccgcttt gaatttgcct ttaggcacgg tgttaagacc tgagttgctt
     9841 aaagccgtcc cggtgatcat gcagggacaa accgtgcagt tgaacagccg tggcgaagga
     9901 tttgtgatta ctgctgatgg cactgccatg caaaccgcaa atgttggcca agtggtagat
     9961 gtcaaagtga tgagtgggca gattgtcaaa gggattgctc aagcgtccgg taaagtagat
    10021 gttcgctttt agtgagtctg gcaacgtttt ttaattgaaa aatgtctgaa aattataaac
    10081 atgcgataaa aagtatatta agcactaaag ttcgcttaaa ctatgccgat agtactgtta
    10141 acgaatgagg gttttgactt tgataagccc tgacacaatt caaaatcaga agaggtacgg
    10201 catgaacatt tcagattcga ttaaaaaaat gggtgttggc gtagacaagc cgacattgga
    10261 aaaaggcgct gcgaataaaa gcagcgagaa agcagcgagc acatccactg cctcttcaga
    10321 caatgtgact ttatcttctg cctcagtgca gttgcagtca ttagaagctg gattggcctc
    10381 tggtgagg
//
LOCUS       DATMSK010000083         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_194636,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000083 DATMSK010000000
VERSION     DATMSK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194636"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VNZ84_02920"
     CDS             <1..277
                     /locus_tag="VNZ84_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWU65934.1"
                     /translation="FHTAKEKVVIPVSGPMTVNDAGTLHSVCLAGVAIAQTMSFGISE
                     LLAEGRLVNLFPAWSEERFPLYALYPSRAHMPLKTSVFLDFVRGLKS"
     gene            complement(339..1187)
                     /gene="asd"
                     /locus_tag="VNZ84_02925"
     CDS             complement(339..1187)
                     /gene="asd"
                     /locus_tag="VNZ84_02925"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987332.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HWU65935.1"
                     /translation="MKLAILLQYIAPKQLLTAAAGKLAHWHAGGLTTAFIGWFVRKYH
                     VNMEEALNGDIASYASFNLFFTRPLKPDARPLANNAFVCPVDGAISQFGKVEQGQIFQ
                     AKGHHYTAQALVGGDAALAANFDSGSFACLYLSPKDYHRIHMPCDGKLLSMTYVPGDL
                     FSVNPLTATNVPGLFARNERVVCTFESAQHGKFVMVLVGATIVGSMATVWHDAKDHII
                     NPPRPGKIIYWHYSDRSITLKQGEEMGRFLLGSTVVMLFEKDVLDFNASWHPGKPIRL
                     GEAMGK"
     gene            complement(1361..2377)
                     /gene="ilvC"
                     /locus_tag="VNZ84_02930"
     CDS             complement(1361..2377)
                     /gene="ilvC"
                     /locus_tag="VNZ84_02930"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWU65936.1"
                     /translation="MNVYYDKDADLSIIKGKKVTIVGYGSQGHAHAANLRDSGVNVTI
                     GLRKGGGSWAKAEGAGHTTLEIAAAVKEADVVMVLLPDETMAEIFNAEIAPNLKKGAA
                     LAFAHGFNIHYNQIVPRADLDVIMIAPKGPGHTVRSEYLKGGGVPSLIAVYQNTSGKA
                     KDIALSYAAANGGTKGGVIETDFREETETDLFGEQAVLCGGAVELVKAGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPQVINSQSKDAMRQ
                     CLANIQNGNYAKQFILEGRTNYPEMTARRRLNAAHPIEQVGNKLRAMMPWIAKNKLVD
                     QSKN"
     gene            complement(2430..2921)
                     /gene="ilvN"
                     /locus_tag="VNZ84_02935"
     CDS             complement(2430..2921)
                     /gene="ilvN"
                     /locus_tag="VNZ84_02935"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWU65937.1"
                     /translation="MRHIISLLMENEAGALSRVAGLFSARGYNIESLTVAVTEDPTLS
                     RMTIVTTGSDDVIEQIIKQLNKLIDVVKVLDLNDGKHIERELMLVKVKATGVHKDEMK
                     RMCDIFRGRIIDVADNSYTIELTGSCSKLDAFIEAIDRSAILETVRTGASGIGRGDRI
                     LKV"
     gene            complement(2921..4684)
                     /gene="ilvB"
                     /locus_tag="VNZ84_02940"
     CDS             complement(2921..4684)
                     /gene="ilvB"
                     /locus_tag="VNZ84_02940"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HWU65938.1"
                     /translation="MDASAHSATDTPAKSMTGADIVVSCLNEEKVKFVFGYPGGAVLH
                     IYDALYKQNGFKHILVRHEQAAVHAADAYSRATGDVGVALVTSGPGATNAVTGIATAY
                     MDSIPLVVISGQVPVPAIGLDAFQEVDMVGVTRPCVKHNFLVKHVKDIAPTIKKAFFI
                     AKTGRPGPVVVDIPKDITAHVADFIYPKTVEMRSYNPILKGHSGQIKKAVKLLLGAKR
                     PMIYTGGGLVLGNGAAELVKLAKALNFPVTNTLMGLGGYPSTDKQFVGMLGMHGTYEA
                     NMAMQHCDVLLAVGARFDDRVIGNPKHFGSQKRTIIHIDVDPSSISKRVKVDVPIVGD
                     VQSVLADMNEILAQEENQTTDTADWWTQINEWRGIQSLDYVYSDSVIKPQYVVQKLWE
                     VTKGEAYVTSDVGQHQMWAAQYYKFDKPRRWINSGGLGTMGVGLPYAMGCQFADKHAQ
                     VACITGEGSIQMNIQELSTCAQYGLPIKVILLNNRYLGMVRQWQEFFYENRYSESYMD
                     SLPDFVKLAESYGHIGMRITNPADVEGALKEAFDRKDKFVFMDFLTDQTENVFPMVEN
                     GKGLSEMVLSPSTASRLKGEE"
BASE COUNT         1082 a         1213 c         1235 g         1288 t
ORIGIN      
        1 gtttcataca gcaaaagaga aagtggtgat tccagtcagt ggaccgatga ctgtgaatga
       61 tgcagggaca cttcatagcg tttgcctggc aggcgttgcg atcgcccaaa cgatgagttt
      121 tggaatcagc gaattattgg ccgaggggag gttagtgaac ttgtttccgg cttggtcgga
      181 agagcgattc cctctgtatg cgctctatcc aagtagggcg catatgcctt tgaaaacaag
      241 cgtgttccta gattttgttc gaggcttgaa aagctaaata gcgatgcaga ttgagatagg
      301 tagttgctat tgatcttgcc gaaacctcgg atatgtcgtt atttccccat ggcctcaccc
      361 aggcgtatcg gtttgcctgg atgccagctg gcattaaagt ccagcacgtc tttttcaaat
      421 aacatcacca cggtagagcc caacaggaag cggcccattt cttcgccttg cttcaacgta
      481 atgctgcggt cactgtaatg ccaatagatg attttgccgg gacgtggcgg gttgataatg
      541 tggtctttgg catcgtgcca gaccgttgcc atgctgccga caatcgtagc accgaccagt
      601 accatgacaa acttgccatg ctgggcggat tcaaatgtac agaccacgcg ctcgttacgc
      661 gcaaacaggc cgggcacgtt ggtggcggtg agtggattca ctgaaaataa atcgccaggc
      721 acatacgtca tgctcagcag tttgccatcg catggcatat ggatacggtg atagtctttc
      781 ggacttaaat acaggcaggc aaaactgcca ctgtcaaagt tcgcagctaa agcagcgtca
      841 ccaccgacca atgcttgcgc ggtgtaatga tggcctttgg cctggaagat ctggccttgt
      901 tctaccttgc caaactggct gatggcgcca tccaccgggc aaacaaacgc attattcgcc
      961 aacgggcgcg catctggttt gagtggacgg gtaaagaaca ggttgaatga ggcgtaactg
     1021 gcaatgtccc cattaagcgc ttcttccatg ttcacatggt atttgcgcac aaaccagccg
     1081 ataaaagccg tggtcagtcc acccgcgtgc cagtgtgcca gtttgccggc cgcggcagtc
     1141 agtaattgtt tgggtgcgat gtattgcagc agaatggcga gtttcacttc tatcccttat
     1201 ctctcagttt aagtgtgttg caagcacttg tttttgtcgt gtctttactc gtgattttac
     1261 aaataaaaaa gcacaggcga tatatcggcc tgtgcttttt acctatggat ggattaattt
     1321 ggcatcaacc caacgtgagg tactgatttc gtatctaaaa ttagttttta gattgatcaa
     1381 ccagtttgtt tttcgcaatc caaggcatca tggcgcgcag tttgttaccc acttgctcga
     1441 tagggtgagc agcgttcaaa cgacgacgtg cagtcatttc tgggtagttt gtgcgacctt
     1501 ccaggatgaa ctgtttagcg tagttaccat tctggatgtt agccaggcac tgacgcattg
     1561 catctttgga ctggctgttg atcacttgtg ggccagttac gtactcacca tactccgcgt
     1621 tgttggagat ggagtagttc atgttggcga taccgccttc gtacatcagg tcaacaatca
     1681 gtttcagttc gtgcaagcac tcgaagtaag ccatttctgg tgcataaccc gcttcaacca
     1741 aggtttcgaa acctgcttta accagttcaa ccgcgccacc acacaataca gcttgttcgc
     1801 cgaacaagtc agtttctgtt tcttcacgga agtcagtttc aatcacgcca cctttggtgc
     1861 cgccgttcgc tgcagcgtaa gacaatgcaa tgtctttagc tttgccagaa gtgttttggt
     1921 acacagcgat caatgaaggc acgccgccgc ctttgaggta ctctgaacgc actgtgtggc
     1981 ctgggccttt aggtgcgatc atgattacgt ccaggtcggc gcgtggaacg atctggttgt
     2041 aatggatgtt gaagccgtgt gcgaatgcca gtgcagcgcc ttttttcagg ttaggtgcga
     2101 tttcggcatt gaagatttct gccatggttt cgtctggcag caataccatg acgacgtcag
     2161 cttctttaac cgctgcagcg atttccagag tcgtgtggcc agcgccttca gctttagccc
     2221 atgagccgcc gcctttacgc aaaccgatag tgacgtttac gccgctgtcg cgcaggttag
     2281 ctgcgtgcgc atgaccttgg gaaccataac ccacgatagt tacttttttg cccttgataa
     2341 tgcttaagtc ggcgtccttg tcgtaataaa cgttcatctc tgcttttgcc tttcaataca
     2401 aatgcttttg tctgtaaaaa taatgtcgat taaaccttga ggatgcggtc gccacgacca
     2461 atgccggacg cgccggtgcg cacggtttct agaatcgcgc tgcggtctat ggcttcaata
     2521 aacgcatcca gtttgctgca agagccggta agctcaatgg tgtagctatt gtcggccaca
     2581 tcgataatac ggccgcggaa aatgtcgcac atacgcttca tttcgtcttt gtggacgccg
     2641 gtggctttca ccttaaccag catcagttcg cgctcaatgt gcttgccatc gttgaggtcc
     2701 agcaccttga ccacgtcaat caacttgttg agttgcttga taatttgttc aatcacatca
     2761 tcggagcctg tcgtcacaat ggtcatgcgc gacagggtcg ggtcttcggt gaccgccacc
     2821 gtgagtgatt caatgttgta accacgtgca gaaaacaggc ccgccacgcg tgacagggcg
     2881 cctgcttcgt tttccatcag cagcgagatg atgtgacgca ttattcttcc cctttcaggc
     2941 ggctggccgt gcttgggctc agtaccattt ctgacaagcc cttgccgttt tcaaccatcg
     3001 ggaagacgtt ttctgtttga tcagttaaaa agtccataaa tacgaatttg tcttttctgt
     3061 cgaacgcctc tttaagggcg ccttctacat ctgccgggtt agtgatgcgc atgccgatgt
     3121 ggccataaga ctcggccagt ttgacgaagt caggcaggct gtccatgtaa gactctgagt
     3181 agcggttctc gtagaagaac tcttgccact ggcgcaccat gcccaggtat cggttattca
     3241 acagaatcac cttgatcggc aggccgtact gtgcacaggt agacagttcc tggatattca
     3301 tctggatact gccttcaccg gtgatacagg ccacttgtgc gtgcttgtcg gcaaactggc
     3361 aacccatggc gtatggcaag ccgacgccca tggtgcccag gccgccggag ttgatccagc
     3421 gacgtggttt gtcaaatttg tagtattgcg ctgcccacat ctggtgctga ccgacgtcag
     3481 aggtcacata ggcttcacct ttggtgactt cccacagttt ctgtaccacg tattgcggct
     3541 tgatgacgct atctgaatat acgtaatcca gagactggat gccacgccat tcgttgatct
     3601 gtgtccacca gtcagcggta tctgtggtct ggttttcttc ctgggccaag atttcgttca
     3661 tgtcagccag caccgactgt acatcgccca caatcggcac atcgaccttc acgcgttttg
     3721 agatactgga tggatccacg tcgatgtgga tgatggtgcg tttttggcta ccaaaatgtt
     3781 ttggattgcc aattacgcga tcgtcaaagc gtgcacccac ggccagcagc acgtcacagt
     3841 gttgcatggc catattggct tcgtatgtac cgtgcatgcc cagcatgccg acaaactgtt
     3901 tgtcggtcga aggatagcca cccaagccca tcagggtgtt ggtcacaggg aaattcaagg
     3961 ctttggccag ttttaccaat tcagctgcgc cattacccaa taccaggcca ccgccggtat
     4021 agatcattgg acgttttgcg cccagcagca gtttgaccgc tttcttgatc tggccgctat
     4081 gacctttaag aatcgggttg taagaacgca tctctaccgt tttcgggtag ataaagtctg
     4141 ccacgtgcgc agtgatatct ttaggaatat ccactaccac cgggcctgga cgacctgtct
     4201 tggcgataaa gaaggctttc ttgatggtcg gcgcaatatc cttaacgtgt ttgaccagga
     4261 agttatgttt gacacaggga cgggtcacgc caaccatgtc gacttcctgg aaagcatcca
     4321 gaccaatggc tggaaccggc acctgaccgc taataaccac caggggaatc gaatccatat
     4381 aggcggtcgc aatcccggtc acggcattgg ttgcgcctgg gccactcgtc accaaggcga
     4441 cgccgacatc acccgttgca cgtgaataag catctgccgc atgtacggct gcctgttcgt
     4501 ggcgcaccag aatatgtttg aaaccattct gtttgtagag tgcgtcgtaa atgtgcaaga
     4561 ccgcgccacc tggatagcca aagacgaatt tgactttttc ctcattcagg caggagacaa
     4621 cgatgtccgc acctgtcatg gatttggcgg gggtgtctgt cgcggagtgg gctgaagcgt
     4681 ccataaaacc ttgatttttt ggctaattaa tgttgaaccc tatacattag cttgctttgc
     4741 cagtttggtc aagattgttg cggatgtgca cgatgctttg ctgcgattca gggtcattta
     4801 aggcaatgtg caaacaag
//
LOCUS       DATMSK010000084         8828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_406086,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000084 DATMSK010000000
VERSION     DATMSK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406086"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VNZ84_02945"
     CDS             complement(<1..1162)
                     /locus_tag="VNZ84_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWU65939.1"
                     /translation="MFDSLIHLLLLLTSSVFAVGLFRILGLPAMLAYFMIGIVLGPHA
                     LGLLEDEESGRQVAEFGIVFLMFSIGLEFSLPKLYAMRKTLFGLGGGQVVLTLLASLV
                     LGKLAGLSLTSAFIIGAAITMSSTAIVSKILMERVDLNSRHGRLSIGVLLFQDLAVIP
                     ILVLIQTLGSHSDNWLDVLGLTLFKSSVLLFLLFKFGKNILNFWFELVAKQRSRELFV
                     LNVLMVTLLMAAATNFVGLSYALGAFVAGMLISETKYRYQVESDIAPFRDILLGLFFI
                     SVGMLLNVSVLFDNILLIILLLIFFLFFKIGLIAILTKFYGFEMGVGVRTGIILGQAG
                     EFSFVVLALGLQTKLIDGEALQLVLSVAVLSMLVAPFIIQYNGRIARALVKSY"
     gene            1387..2388
                     /locus_tag="VNZ84_02950"
     CDS             1387..2388
                     /locus_tag="VNZ84_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HWU65940.1"
                     /translation="MNTAKSGVRLVKSPDIVTRARGILLHEADEIRQLADRLGSAFEA
                     AVNLILDCRGRIVVTGMGKSGHIGGKIAATLASTGTPAFFMHPAEASHGDLGMITAED
                     VVIALSNSGESDEIINILPAIRRIGARILSITGSEDSTLSRESEVHLSAAVSHEACPL
                     GLAPTASTTAALALGDALAICVLDLREFTAEDFARSHPGGSLGRRLLVRIKDVMRTRD
                     AIPYIYEQATVQDAITQISSKGMGFTAIVDANLHPVGVFTDGDLRRLFLQGETHTETA
                     IALVMNKHPRLLGTQQMAVEAVNLMEQYKINGFLVVDEQQQLVGAFNMHDLLKAKVV"
     gene            2415..3047
                     /gene="lptC"
                     /locus_tag="VNZ84_02955"
     CDS             2415..3047
                     /gene="lptC"
                     /locus_tag="VNZ84_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HWU65941.1"
                     /translation="MLKHPILLPIALMLFLALLTFWINQTVQEQGLSMSRLSRHDPDY
                     MLHNFVSTRTNAAGMTKYVLAAVEMRHFPDNDYTELKRPRFTQFGLDKPYTQIYGQRG
                     KISANGKLVEFNNRVKVVRQGTVQKGEMQLHTERLTMEPDTEIAYTDLPVTIYQKPAT
                     VITGTGMRFDNKAETMQLFNRVHVHYERAPVVTKPAIPTAPAKAGSRRVR"
     gene            3061..3714
                     /gene="lptA"
                     /locus_tag="VNZ84_02960"
     CDS             3061..3714
                     /gene="lptA"
                     /locus_tag="VNZ84_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HWU65942.1"
                     /translation="MNTQKPLFYAACLIFCALQWSGSALAEESDRDQPIELEADTVTV
                     NDAKKISVYTGNVILNQGTLQIKADKMIVREDQDGFQHSTCTGNPTTFKQKRTGKDEW
                     MQGSGQRIEYNARMDKVQLYTNAWVKRGEDVVTGDYISYDANAEYAEVIGGTKANPNG
                     TAGSRVKATIQPKNKTTPPVIDNKPNSQQGLRMNRSLQLKVEPVPSDAGSEPKKEQP"
     gene            3729..4475
                     /gene="lptB"
                     /locus_tag="VNZ84_02965"
     CDS             3729..4475
                     /gene="lptB"
                     /locus_tag="VNZ84_02965"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HWU65943.1"
                     /translation="MQTTASSEHNHLYVSRLKKTYQGRTVVKNTTLELHSGEVIGLLG
                     PNGAGKTTSFYMIVGLVALDDGEISLNGADVSHSPMHERARMGLAYLPQEASIFRKMS
                     VTDNIMSILETRPYTDEEREARLESLLDQLHIQHIRNSQAVSLSGGERRRVEIARCLA
                     TDPKFILLDEPFAGIDPIAVIEIQKIIRYLSSQNIGILITDHNVRETLDICDRAYIVN
                     EGAVFAAGTPDEIIQNEGVREVYLGKNFRL"
     gene            4482..6032
                     /locus_tag="VNZ84_02970"
     CDS             4482..6032
                     /locus_tag="VNZ84_02970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWU65944.1"
                     /translation="MKQNLQLRISQNLALTPQLQQSIRLLQLSTLELSQELESILQEN
                     PLLEMADGEEGEFEDNSATPTENIESVHADDANSFDLATQQEITAPAETLREDLHDEL
                     GSNEGELANLSEEFNPPEFEDNYEEFGSTSNWDEAGRNNLDDEDSDFSRQDANNISLR
                     EHLLDQIQLAHLSQRDMTLVKLLLDSINDDGYLEQDLQEIVEHLPLELEVELLELETA
                     LKLIQNLDPIGVGARNLRECLLLQLQHLPAETPYLRTAMALVKDHLALLANKDFVKLR
                     KVLSCDETVLKGAQQLIRQQNPKPGSEFATFSHDHFIQHDVVVKKIKGIWVASLNDGV
                     IPKLRINQLYADILKRNRESSGQYLQSQMQEAKWMIKNIQQRFSTILRVSQAIVDRQR
                     NFFEHGEIAMRPLVLREIAEELDLHESTVSRVTTHKYMLTPRGVYELKYFFGSSVATD
                     AGGSCSATAIRALIKQMVAEENPKKPLSDNQITDTLAQQGIVVARRTIAKYRESLNIP
                     PANLRKSL"
     gene            complement(6080..7318)
                     /gene="tyrS"
                     /locus_tag="VNZ84_02975"
     CDS             complement(6080..7318)
                     /gene="tyrS"
                     /locus_tag="VNZ84_02975"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWU65945.1"
                     /translation="MTDVTQPSAAAEVLSPEIVHALAIIKRGADELLIEQELVEKLKT
                     GKPLRVKAGFDPTAPDLHLGHTVLINKLRQLQDLGHQILFLIGDFTGMIGDPTGKSTT
                     RPPLTVEQVKANADTYSEQVFKILDPAKTEIVFNSKWLSDLGAAGMIKLAASHTVARM
                     LERDDFSKRFKGNQPIAIHEFLYPLLQGYDSVALQADLELGGTDQKFNLLMGRELQKQ
                     AGQSQQCVLMMPLLEGLDGVNKMSKSLGNYIGISEAPETIFAKIMSISDALMWRYIDL
                     LSFASLETIAQWKAQVAGGENPRNIKVGFAQEIVARFHGQTAAEKALQDFQTRAKGGI
                     PDDVPEVAVTIEGDSIGISQLLKQAGLVESTSEAMRAVQQGGVKLDSVKVDDKNLPVA
                     KGTTVVAQVGKRKFAKITIQ"
     gene            7435..8562
                     /locus_tag="VNZ84_02980"
     CDS             7435..8562
                     /locus_tag="VNZ84_02980"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWU65946.1"
                     /translation="MNSPALPTSTRLFIGLMSGTSLDGVDAVIVSQHGEQVTQQGHYF
                     VPYPAELKQSLLDLHTPALDEIHQAAVISNRLAELYAQAVKELLGESSLRASEVTAIG
                     CHGQTIRHCPDLPNGQAYTLQLGNHARLTELTEITVVGDFRSRDIAAGGQGAPLVPAF
                     HQAVFAASNKHRVIINIGGIANLSDLPVNGHVTGFDSGPGNLLMDSWTLQHTGNPYDA
                     SGAWAVRGDINQPLLQAFLADPYFVLPIPKSTGRDLFNQAWLNKHLQAHPDTPQNVAR
                     TLLELTAVTIAQSLQQYCPGAEEVYVCGGGAHNLALIDRLQALCGLSIQRTDVLGVDS
                     DWVEAVAFAWLAQRCIDHLPGNIPAVTGAAGPRVLGAIYPA"
BASE COUNT         2428 a         2263 c         2085 g         2052 t
ORIGIN      
        1 tgtaactctt gaccagcgcg cgggcaatgc gcccgttgta ttgaataata aatggcgcca
       61 ccagcatgga aagcactgcc acagacagta ccagttgcaa ggcttcccca tcgatcagct
      121 tagtttgcaa gcccaatgcc aggaccacaa agctaaactc gccggcctgg cccaaaatga
      181 tgccggtacg gacgccgacg cccatttcaa acccgtaaaa cttggtcaat atagcgatca
      241 aaccaatctt gaaaaacaag aagaagataa gcaacaggat gatgagtaga atgttgtcaa
      301 acaacacgga cacattcagc aacataccca cactgataaa gaacaggcca agtaaaatat
      361 cgcggaacgg agcaatatca gattccacct ggtagcgata tttcgtctct gaaatcagca
      421 tgccagcgac aaacgcaccc aatgcatatg acaagcctac aaaattagtg gcagctgcca
      481 tgagtagcgt caccatcagc acgttgagca caaacaattc gcgtgaacgt tgcttggcta
      541 ccagctcaaa ccagaagttc aggatatttt taccgaattt gaatagcaaa aacagcagta
      601 ctgaagactt gaataaggtc aggcccaaca catccagcca attgtcgcta tgcgacccca
      661 atgtctggat caacaccagg atcgggatca cagcgaggtc ttgaaacaac aacacgccaa
      721 tactcaagcg gccatgcctg gaatttagat ccacgcgttc catgaggatt ttggacacaa
      781 tcgcggttga agacatggtg attgcggcac cgataataaa ggctgaagtc aaactgagcc
      841 ccgctagctt gcccagtacc aggctggcca gcaacgttaa taccacctgg ccgccgccca
      901 aaccaaacaa ggttttgcgc atggcataca atttaggcag actgaattcc agaccgatgc
      961 tgaacatgag gaacacgatg ccgaattctg caacctggcg accactctct tcatcttcca
     1021 gcaagcccaa cgcatgtggg cccaacacaa taccgatcat gaaataggcc agcatcgctg
     1081 gcaaacccag gatacggaac aagcccacgg caaacaccga gctggttagt agtaataata
     1141 aatggataag ggaatcaaac atgcatcagc tctaagcact tatgccaggc cagaaaagat
     1201 gtggacctga tcattaatta attgagggtt tggtcaggat gtattctgaa acaaaattgt
     1261 ttcaagttga tgcaacctgc cataaaacac tattttatgc aaaaaatgtg cctaataata
     1321 aaaaaaccgt aaaaaaatag tgttttactt tattgcgagg ggcctcactt gttatactaa
     1381 ctcgccatga acacagcaaa atctggtgtc cgtcttgtaa aaagtcctga tatcgtgacc
     1441 cgcgcacgag gcatactatt gcacgaagcg gatgaaatcc gccaattggc cgaccgatta
     1501 ggcagtgcct ttgaagctgc tgtcaacttg attcttgatt gccgcggccg catcgtagtg
     1561 acaggcatgg gaaaatccgg gcacattggc ggcaagattg cagccacact cgccagcaca
     1621 ggcacccccg cttttttcat gcatccggct gaagccagtc atggtgacct gggcatgatt
     1681 acggctgaag atgtcgtcat tgcgttatcc aactccggag agagcgatga gatcatcaat
     1741 attttgccag ccatccgccg tattggcgca cgcattttga gcatcaccgg tagcgaagat
     1801 tcaaccttgt cacgtgagtc tgaggtgcac ttaagtgcag ctgtcagcca cgaagcctgc
     1861 cctctaggac tagctcccac agcaagcacc acggccgctc tagccttggg cgatgcgctg
     1921 gccatctgcg tactggattt gcgcgaattt acagctgagg atttcgcacg ctcacaccct
     1981 ggaggcagct taggccgacg gttgctggtc aggattaaag atgtcatgcg tacccgggat
     2041 gcgatcccat acatttatga acaagcaacg gttcaggatg ccattaccca gatttcgagc
     2101 aaaggcatgg gctttacagc gattgtggat gccaaccttc atccggttgg cgtttttacg
     2161 gacggggatt tacgccggtt gtttttacag ggcgaaaccc atactgagac tgcaatcgca
     2221 ctggtgatga ataaacaccc ccgcttgtta ggcacgcaac aaatggcggt tgaagcagtc
     2281 aacctgatgg agcaatataa gattaatgga tttttagtgg tcgacgagca acaacaattg
     2341 gtaggcgcat tcaatatgca tgatttactt aaggcgaaag tcgtttaagc cattgggcat
     2401 tctggaattc acctatgctt aaacacccga ttctgctgcc aattgcatta atgctgtttt
     2461 tagcgctgct gacattctgg attaaccaga cggtccagga gcaaggactc agcatgagcc
     2521 gcttgagccg ccatgatcca gactacatgt tgcataactt tgtcagcacc cgcactaacg
     2581 ctgctgggat gacgaaatac gtgttagcag ccgtagaaat gcgccacttt cctgacaatg
     2641 actatactga attaaaacgt ccacgtttta cccaatttgg gctggacaaa ccttacaccc
     2701 aaatttatgg tcagcgtgga aaaatttccg ccaatggtaa actggtggaa tttaacaacc
     2761 gggtaaaagt cgtccgccag ggcacagttc aaaagggtga aatgcaatta cacactgaac
     2821 ggcttaccat ggagcctgat acggaaatcg cctataccga cttacctgtc acaatttacc
     2881 aaaaaccggc aaccgtcatt accggcacgg gcatgcggtt tgataacaag gcagaaacca
     2941 tgcaattatt taaccgtgtg catgtacatt atgagcgtgc accggtcgta acaaaacctg
     3001 caatcccgac agcgcctgcc aaagccggca gccgaagggt tcgttgatct ggagagatga
     3061 gtgaacaccc aaaagccatt attttatgcc gcatgcttga tcttctgtgc cttacagtgg
     3121 tcaggcagcg cgttggctga agagtcagac cgtgaccagc ccattgagct ggaagcggat
     3181 accgtaacgg tcaatgatgc caagaaaata agtgtttata caggcaatgt tatcctcaac
     3241 caggggactt tacagatcaa ggctgacaaa atgattgtgc gtgaagacca ggatggtttt
     3301 cagcatagta cctgtaccgg caatccaacc accttcaagc aaaagcgtac aggcaaggat
     3361 gagtggatgc agggcagtgg ccagcgtatc gagtacaacg cccgtatgga caaggtacaa
     3421 ctgtatacca atgcctgggt caagcgtggc gaagatgtcg tcaccgggga ctacatcagc
     3481 tatgacgcga atgcggaata tgctgaagtg attggcggca ctaaagccaa cccgaatggc
     3541 acagcaggta gccgggtgaa agctaccata cagccaaaaa acaaaaccac cccgccagtc
     3601 atcgacaata aaccgaattc acagcaagga ctgcgaatga atcgatcact tcagcttaag
     3661 gttgagcctg tacccagcga cgcgggtagc gagcctaaga aagagcaacc ttaaagacga
     3721 ttcgccatat gcagacaacc gcttcatcag aacataatca tttgtacgtc agccgcctga
     3781 aaaaaactta tcagggccgc accgtggtca aaaacaccac gctagagttg cacagtggcg
     3841 aagtcattgg gttactgggc ccgaatgggg ctggcaaaac gaccagtttt tacatgattg
     3901 tcggactcgt ggcactcgac gatggtgaaa tcagcctcaa cggggctgac gtgagtcact
     3961 caccaatgca tgaacgcgcc cgcatgggat tggcctacct gccacaggaa gcttccattt
     4021 tccgcaaaat gtccgtcact gataacatca tgtcaatcct ggaaacgcgc ccttacacgg
     4081 atgaggaacg cgaagcgcgg cttgagtctt tgctggatca actacatatc cagcatatcc
     4141 gcaacagcca ggctgtcagc ctgtctggcg gtgaacgacg ccgggtagaa attgcgcgct
     4201 gcctagcgac ggacccgaaa ttcatcttgc tggatgaacc ctttgccggg attgatccga
     4261 ttgcggtgat tgaaatccag aaaatcattc gctacctgag tagccagaat atcggcattt
     4321 tgattaccga ccataacgta cgtgaaacac tggacatttg tgaccgggct tacattgtga
     4381 atgagggtgc tgtgtttgcg gccgggacgc ccgacgaaat catccagaat gaaggcgtac
     4441 gtgaagtcta cctgggtaag aactttaggc tgtaatttgt tatgaagcaa aacctgcaat
     4501 tacgcatttc tcaaaacctg gcactgacac ctcagttgca gcagtcgatt cggcttctgc
     4561 aactctcgac actggaactg agccaggaac tggaaagcat tctgcaagaa aatcccctgc
     4621 tggaaatggc tgacggtgaa gaaggtgagt ttgaagacaa ttcagcaacc cctaccgaaa
     4681 atattgaatc agtccatgca gacgatgcga actcgtttga tctggccacc cagcaagaaa
     4741 tcactgcgcc tgccgagacg ctgcgcgaag acctacatga tgaactaggc agcaatgaag
     4801 gcgaactggc caatctcagc gaagaattca atccacccga atttgaagat aattacgaag
     4861 agtttggcag caccagcaac tgggatgagg ctggccgcaa taatcttgac gatgaagaca
     4921 gtgatttttc acgccaggat gccaacaata tcagcctgcg cgagcacctg ctggaccaga
     4981 ttcaactggc gcacctgtca cagcgtgaca tgaccctggt caagttactg ctcgatagca
     5041 ttaatgatga tggctacctt gagcaagact tgcaggaaat tgttgaacac ttgccgcttg
     5101 agctagaagt tgagttactc gaactggaaa ccgcactcaa actgatacag aatctggacc
     5161 cgattggtgt cggcgcccgt aacttacgcg aatgcctatt attgcagttg caacacttgc
     5221 cggcagaaac accctattta cgtacagcca tggctctggt caaggatcac ttagccttgc
     5281 tggccaataa ggattttgtc aaacttcgca aagtgctcag ttgtgatgaa acagtcctca
     5341 aaggagcgca gcaactgatc cgtcagcaaa accccaaacc tggcagcgag tttgctactt
     5401 ttagccatga tcattttatc cagcacgatg tcgtcgtcaa aaaaatcaaa gggatctggg
     5461 tcgcctctct aaacgatggc gtgattccaa agctgcgtat caaccaactg tatgctgaca
     5521 tcctcaaacg caaccgtgaa agctcaggcc agtacttaca aagccagatg caggaggcga
     5581 agtggatgat caagaacatc cagcagcgtt tttcaaccat tttgcgcgta tcgcaagcga
     5641 ttgtagaccg tcagcgtaat ttctttgaac atggcgagat tgccatgcgg ccattggtac
     5701 tgcgcgaaat tgccgaagag ctcgatttgc atgagagtac ggtttcacgt gtgactaccc
     5761 ataagtatat gctcacaccg cgtggggtct acgagctcaa atatttcttt ggcagttcag
     5821 tggctacaga cgccggaggt tcttgctcag cgacggcaat ccgtgcgtta atcaagcaaa
     5881 tggtggcaga agaaaatccg aaaaaaccgc tctcggataa tcagattaca gacacgctgg
     5941 cacaacaagg cattgtggtg gcgcgccgca cgattgccaa ataccgtgag tcactgaata
     6001 ttccacctgc caatttacgt aaatcactct aaaaaataaa ggccgcaaca gcggcttttt
     6061 gtttgtgatg aagcaggttt tattgaatgg taattttggc aaacttgcgc ttaccgactt
     6121 gcgccaccac agttgtccct tttgcgacag gcaggttttt atcgtccact ttcacgctat
     6181 ccagtttgac accaccctgt tgcacagcac gcatggcctc actggtactc tccactagac
     6241 cagcttgctt cagcagttgg ctaatgccta tgctatcacc ttcaatggtg acggccactt
     6301 ccggcacatc atcaggaata ccgcccttgg cacgagtctg gaaatcctgt agggcttttt
     6361 ccgctgcagt ctgaccatga aaacgcgcca cgatctcttg tgcaaacccc actttgatat
     6421 tacgtggatt ttcaccaccc gcgacctggg ccttccattg cgcaatggtt tctaatgacg
     6481 caaaggataa taagtcgata tagcgccaca ttaacgcatc agaaatggac atgattttgg
     6541 caaaaatcgt ctcaggtgct tcactgatcc caatatagtt acccagtgat ttggacatct
     6601 tgttgacgcc atccagccct tccagcaaag gcatcatgag gacacattgt tgtgactggc
     6661 cggcctgctt ctgcaactcg cgacccatca gcaggttgaa tttctggtca gtaccgccca
     6721 gctccaaatc ggcttgcaat gcgaccgagt catagccttg caacaaggga tacaagaact
     6781 catgaatcgc aatcggctgg ttgcctttaa aacgcttcga gaagtcgtca cgctccagca
     6841 tgcgcgccac ggtgtgactg gcagccagct taatcatacc cgcagcccca aggtcactta
     6901 accacttgga gttaaataca atttcggttt tggctgggtc cagaatctta aatacctgct
     6961 cagaataggt gtccgcattg gctttcacct gttccaccgt taatggtggc cgtgtagtgc
     7021 ttttgccggt aggatcacca atcatgccag tgaagtcgcc aatcaggaat aatatctggt
     7081 gtcccagatc ctgtaactga cgtaatttgt taatcagcac ggtatgcccc agatgcaaat
     7141 caggggcagt tggatcaaaa cctgccttca cgcgtagtgg cttgcctgtt ttcagctttt
     7201 ccaccagctc ttgttcgatc agcagctcat cagcaccgcg cttgataatc gccagtgcat
     7261 gcacgatttc cggacttaaa acttcggcag ctgctgatgg ttgggttacg tctgtcataa
     7321 ggattttctg caacgcgctt ctggcacgct ttgttatcaa gttaaaggcg ctattttaac
     7381 gtaatcccgc acttttctgt atgcaacaca tcagcagtgt taaacttatg ccttatgaac
     7441 tcccctgctt tacctacttc aacacggctg tttattggtc tgatgtccgg caccagcctg
     7501 gatggggtag atgcagtgat cgtctcacaa cacggtgaac aggttacgca acaagggcac
     7561 tattttgtgc cttatccagc agaactcaag cagtcattac ttgatttgca taccccagcg
     7621 ctggatgaaa tacaccaggc agctgtgatc tccaaccgcc tggctgagct atacgcacaa
     7681 gcagtaaaag agttactagg cgagtcttca ctccgcgcta gcgaggtgac tgcaattggc
     7741 tgtcacggtc aaaccatacg ccattgccct gatttgccaa acggacaggc ctataccttg
     7801 caattaggca accatgcgcg cctgacagaa ttaacagaaa tcaccgtagt aggcgatttc
     7861 cgtagccggg atattgccgc aggcggccaa ggcgccccct tagtcccagc ttttcatcaa
     7921 gccgtgtttg cggccagcaa taaacatcgc gtcattatta acatcggcgg cattgccaat
     7981 ttgagtgatt tacctgttaa cggtcacgtc acaggctttg actctggccc cggcaacctg
     8041 cttatggatt cctggacgtt gcaacatacc ggcaacccat acgatgccag cggcgcatgg
     8101 gcagtgagag gcgacattaa ccagccacta ttacaagcct ttttggctga cccatatttt
     8161 gtactgccta ttcccaaaag tactggccgt gatttattta atcaggcctg gctaaacaaa
     8221 cacctgcaag cgcatcccga cacacctcaa aatgtcgcac gcaccttgct agagctcact
     8281 gcagtgacta ttgcacagtc gttacagcaa tattgtcccg gcgcagaaga agtgtatgta
     8341 tgtggtggag gcgcacataa tctggccttg atagacaggt tgcaagcact gtgcggtctc
     8401 tcgatacaac gtactgatgt gttgggtgtc gattcagact gggtggaagc cgttgccttt
     8461 gcctggctgg cgcaacgttg catcgatcat ttgcctggca acatccctgc tgtcaccggt
     8521 gcggcagggc ctcgtgtgtt aggtgccatt tatcctgcat aaaacaaata cactaaagcg
     8581 gtgcaaatgc actctacgtt tgatttaaac tggtgcaaga ttgaagatga ttttttaact
     8641 tttcccgact cattcccgca caatccacca tattcaaaaa cgattgcgta taatttatac
     8701 aaccttgact taaacactga gtaccttatg gctaccaaag caaccattac ctttgacaac
     8761 aaccctgctg cgattgaatt acccgtatta aagggctcgt taggcacgga tgtgattgat
     8821 atccgcag
//
LOCUS       DATMSK010000085         5625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_147487,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000085 DATMSK010000000
VERSION     DATMSK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147487"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /gene="dnaQ"
                     /locus_tag="VNZ84_02985"
     CDS             complement(<1..613)
                     /gene="dnaQ"
                     /locus_tag="VNZ84_02985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HWU65947.1"
                     /translation="MKQIFLDTETTGLYPDQGHRIIEIAAVEIFNRRPTQRHFHIYIN
                     PEREIDPGAQEVHGISLEFLQDKPLFANIAEEFIEFIRGAELIIHNAPFDVGFLNMEL
                     ARLNLDKLESYISGIIDTLKMAKEMRPGQRNNLDALCRHYGIDNSQRTLHGALLDAEL
                     LAEVYIAMTRGQESLLMELSSPVEQVTQASLAVDLSAIKLLAAD"
     gene            complement(630..1070)
                     /gene="rnhA"
                     /locus_tag="VNZ84_02990"
     CDS             complement(630..1070)
                     /gene="rnhA"
                     /locus_tag="VNZ84_02990"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HWU65948.1"
                     /translation="MSNQQWIEMYADGACKGNPGVGGWGVWLKAGPHEKSLYGGEALT
                     TNNRMELTAVIQGLQALKKPSHIKIYTESSYVQKGMTEWLPGWKQKQWRTADKKPVKN
                     ADLWQTLEALAQPHQIEWIWVRGHAGNEGNERADALANQGVLTV"
     gene            complement(1036..1905)
                     /locus_tag="VNZ84_02995"
     CDS             complement(1036..1905)
                     /locus_tag="VNZ84_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU65949.1"
                     /translation="MIVKLHVFGDLLLCAFSESTLSPSRPASSETTALKPTGFEAMAL
                     GQYIAKHETALYAQTVADVFGFHALQIGWPAHDLLENCRIPYKHSLQIAATANASLLS
                     ESEYLPFAEHSVDLVCMPHVLEQCADPQQTLREAFRVLLPEGTLILTGICPFSYLGLR
                     ARFGQFRQAGQFRRLFTVWRLRDWLNVLGFEVVQSGRLVHALPVNDPDWLARQSWLER
                     WGKHSCGATGGVYFLIAKKRVLNVRLLKPEWKKLPLNPALQVRKGHSGIQKQTQKQLE
                     CADVKSTMDRNVR"
     gene            1929..2708
                     /gene="gloB"
                     /locus_tag="VNZ84_03000"
     CDS             1929..2708
                     /gene="gloB"
                     /locus_tag="VNZ84_03000"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HWU65950.1"
                     /translation="MQETSASLEIVPLPAFSDNYLWLIHDGKHALIVDPGDAAVVENA
                     LKTHHLQLDAILLTHHHADHTDGAAALQQHWQCPVYAPNSDHKAYKALQVNRVQAGDY
                     IEFKRFPALSCEILGLPGHTLDHIAYYIDHHHLFSGDVLFGAGCGRLFEGTPTQMFNS
                     LQAIAALPEETLIYPAHEYTAHNLAFAQTIEPGNTQLQQRARETASLRAQQLPTLPTT
                     LRLELATNPFLRCEEISQQDGFKDWPAVDVFTELRSRRNAF"
     gene            2919..4445
                     /locus_tag="VNZ84_03005"
     CDS             2919..4445
                     /locus_tag="VNZ84_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWU65951.1"
                     /translation="MRFKKILLCIALSATPLWAVAEYVEPATLSDDNDSSYDESYGGP
                     ETESYSSSYQFEDSLTLQESLWDRVRDGFNMPDIDSVYTNKHEQWYASRPDYVSRMLE
                     RSKKYLYHIVVEVEKRGMPTEIALLPMIESGFNPKALSTSKASGIWQFIPSTGKDFGL
                     KQNYWKDSRKDVTAATQAALNYLQKLYTMFGAWDLALAAYNAGEGTVARAIERNRRLG
                     LPTDYQHLSLPDETRNYVPKLQAIKNIIFRPEEYGLTLNEIPNKPYFTTVQAPNKIDA
                     KLAAELAGISLEEFNALNPGFTRPVLITENSYQKLLLPTWAASNFTNNLNGYNQPLSN
                     WHSYHPKKGERLGAIASRFGISIYELSATNNLKPNRNLIVRRPLLVPASLQSGLTRPI
                     DVASMAKLSVEVPVEKESSSNSVTISHKVKQGDTLGKLAKRYGMSSKQIMQQNHLKST
                     SLSIGQVLQLDKHQSTSNKTSARSTHKTSATSRGTKSQSSSRKKTSSTHSKKHTLSKH
                     "
     gene            4528..>5625
                     /locus_tag="VNZ84_03010"
     CDS             4528..>5625
                     /locus_tag="VNZ84_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU65952.1"
                     /translation="MATLLCAGLVACSADAPDQSSTAFDQSYPAQSGGTLISAMPSDP
                     SSMIGMVAGESASLAIGSYLFNSLLKYDQNLDLAGELAESWQVADDKKTITFKLKPDL
                     QWADGKPLTSADILFTWQLITDESTHSPYASDYQLVTKAEAPDARTFIVHYSQPFAPA
                     LESWASLQVLPKHKLAGQDIRTTSFAQKPLGSHYYQLKEWRHGEYIKLEKNPKSVLGP
                     AYIDHLIERIIPDPAAQFLELMADNIDSMSMDPITYARIIPSRPALEARLNQFKELGN
                     SYTYLGFNLKRKPFNDVRVRKAINYAIDKQEIIDGVYLGLGITIASPYKPGTRWSNPD
                     LQPYSFDPAKAKQLLIEAGYQDTDGDGIVDRA"
BASE COUNT         1485 a         1503 c         1279 g         1358 t
ORIGIN      
        1 catctgcggc aagtagtttt atcgccgaca gatccacggc caaactggcc tgagtcacct
       61 gctccacagg gctggagagt tccatcagca aactttcctg gcctcgcgtc atcgcaatat
      121 acacttctgc cagcaactca gcatcaagca aggcgccatg caaggtacgc tgcgagttgt
      181 ctatgccata gtgacggcac aaggcatcta agttgttacg ttggcccgga cgcatttcct
      241 tggccatttt cagcgtatct atgatgccgc tgatataaga ttccagctta tccaggttta
      301 acctggccaa ctccatgttg agaaagccga cgtcaaatgg cgcattatgg ataatcagct
      361 ctgcaccgcg aatgaactca ataaactctt cagcgatgtt ggcaaacaga ggtttatcct
      421 gcaggaactc cagcgaaatg ccatgcacct cttgcgcacc gggatcgatc tcccgctcag
      481 gattgatgta aatatgaaaa tgacgctggg tgggacgacg attaaaaatt tccacggccg
      541 caatttcaat aatgcggtgt ccctggtctg gatataagcc tgtcgtttcc gtatcgagaa
      601 aaatctgctt catgaaatac cctatcctct taaactgtca gaacaccttg atttgccagg
      661 gcatctgcac gttcattacc ctcattgccg gcatgcccgc gaacccatat ccactcaatc
      721 tggtgcggct gtgccaaggc ttcgagtgtc tgccataagt cagcattttt gaccggtttt
      781 ttgtctgcgg tacgccactg cttttgcttc catcctggca accattcagt catgcctttt
      841 tgtacatagc tagactcagt ataaattttg atatgggagg gttttttgag cgcttgcagg
      901 ccttgaatga cagccgtaag ctccatacga ttatttgtgg ttaacgcttc accgccatat
      961 agtgactttt catgtggccc ggctttgagc cagacccccc agccacccac accggggttg
     1021 cctttgcaag ccccgtcagc gtacatttct atccattgtt gatttgacat ccgcgcattc
     1081 taactgtttt tgcgtttgtt tttgtatgcc ggaatggcct tttcgcactt gcaaagccgg
     1141 attgagcggc agctttttcc actcaggctt gagcaggcgt acgttaagta cccttttttt
     1201 ggcgatcaaa aagtatacgc caccagttgc gccacagctg tgtttccccc accgttccag
     1261 ccaggattgc cgtgccagcc agtcaggatc attcaccggg agcgcatgta ccagcctgcc
     1321 gctctggacg acttcaaatc ccagcacatt gagccaatcg cgcaaacgcc aaactgtaaa
     1381 taaacgacgg aattgacctg cctgcctgaa ttgaccaaac ctggcacgca accccaggta
     1441 actgaaagga caaatgcccg tcaagatcaa tgtcccttct ggtaacagca cacggaatgc
     1501 ctcgcgcaag gtttgctgtg ggtcagcgca ttgttccagc acatgtggca tacagaccaa
     1561 atccaccgaa tgttcggcaa aagggagata ttcactttcg ctcaacaggg aggcattggc
     1621 ggttgctgct atttggagtg agtgcttgta cggaatacgg caattttcca acaaatcatg
     1681 cgcaggccaa ccaatctgta aggcatgaaa gccgaagaca tcggcaacgg tttgggcgta
     1741 tagcgccgtt tcatgctttg caatatattg gcccaatgcc attgcttcaa agccagtagg
     1801 ttttaaagca gtcgtttcag aggaagcagg ccggctaggt gacaaggtgg actccgaaaa
     1861 tgcacataat aatagatcgc caaatacgtg cagcttgaca atcatgacca ttttttcaat
     1921 gatgcgggat gcaagaaact tctgcaagcc tagaaattgt tccgctaccg gcatttagcg
     1981 acaattattt atggctcata catgacggaa agcacgcact gattgtcgat ccaggcgatg
     2041 ctgcagtggt tgaaaacgct ttaaaaactc accacctgca gctcgatgca attttactca
     2101 cgcatcacca tgccgaccac acagacggtg ccgccgcact gcaacagcat tggcaatgcc
     2161 cggtatacgc acctaactct gaccacaagg cttacaaggc actacaggta aatcgggtcc
     2221 aagccggtga ctatattgag tttaagcgct ttcccgcgct ttcgtgtgaa atcctcggcc
     2281 tgcctggcca tacgctagac cacattgcct actatattga tcaccaccac ctttttagtg
     2341 gtgatgtctt gtttggtgcg ggttgcggtc gcctgtttga aggcacgcct acacagatgt
     2401 ttaattccct gcaagcgatt gcagccttac ccgaagaaac cctgatttat cctgcccacg
     2461 aatatactgc gcacaatctc gcatttgcgc agacgataga accaggcaac actcaactgc
     2521 agcaacgtgc acgagaaaca gcatcgctac gtgcacagca attgcccacc ttaccaacta
     2581 ccctgcgttt agagttggca accaatccat tcttgcgttg tgaggaaatc tcgcaacaag
     2641 acgggtttaa ggattggcca gccgttgatg tgtttaccga gctacgtagc cgccgcaatg
     2701 cattttagac cagcccgttg catacacaaa gtccgccagg ctgcaagggg ttgtctcact
     2761 ttgaaattaa cgtagaatga tgtcctcctt aaggcgctta catgcgttac gccaatcaca
     2821 tgctggttgg cttggcctaa accggccgcc cggatttatc aatgcctgcg cgagttttat
     2881 acgctcgtgc aacagcttca ggactaagta ttaccgtcat gcgttttaaa aaaatattgc
     2941 tatgtatcgc cctttctgct actcccttat gggctgtcgc agaatacgta gaaccggcaa
     3001 cattatcaga tgacaatgac agtagttacg atgaaagcta tggcgggccc gaaacggaaa
     3061 gttacagttc aagctaccaa tttgaagact cgctcacatt gcaggaaagc ctgtgggacc
     3121 gtgtacgtga tggcttcaac atgcccgaca tcgactccgt ttataccaat aaacacgagc
     3181 aatggtatgc ctcccgccct gattacgtct cacgtatgct ggagcgcagc aaaaaatacc
     3241 tatatcacat tgttgtagaa gtcgaaaaaa gaggcatgcc aactgaaatt gcgctgctac
     3301 ccatgatcga aagtggattt aatcccaagg cactttctac cagtaaagcc tctggtatct
     3361 ggcaatttat tccctctacc ggcaaggact ttggcctcaa gcaaaactac tggaaagact
     3421 cacgcaagga tgtgacagcg gccacgcagg ctgcgctcaa ttatctgcaa aagctctata
     3481 ccatgtttgg agcctgggac cttgcgctgg ctgcctataa cgccggcgaa ggaacggttg
     3541 cacgtgcgat agaacgtaac cgccgcctgg gattaccgac tgattaccag catttgtccc
     3601 ttcctgacga aacccgtaat tacgtgccca aactgcaggc catcaagaac attatttttc
     3661 gccctgaaga gtatgggttg acgctgaacg aaattccgaa caaaccttac ttcacgacag
     3721 tacaggcccc taacaaaatt gacgcaaaac tggccgcaga actggctggc atttcgctcg
     3781 aagagtttaa cgcactgaac cctggcttta cccgtccagt actgattact gaaaacagct
     3841 accagaaatt gttattgcca acctgggccg cgagcaactt caccaacaac ctcaatgggt
     3901 ataatcaacc gctgtccaac tggcacagct accaccctaa aaaaggtgag cgcctgggtg
     3961 cgattgccag ccgctttggt atcagtattt atgagctcag tgcgaccaac aacttgaaac
     4021 ccaaccgaaa tctgattgta cggcgcccac tattagtacc agcgagcttg caatccggct
     4081 tgacccgacc aattgatgtt gccagcatgg cgaaattatc cgtcgaagtg ccggtagaaa
     4141 aagaatcctc ctccaattct gttactattt cacataaagt gaaacaaggg gacacgctgg
     4201 gaaaactggc gaagcgctac ggtatgagtt ccaagcagat catgcagcaa aatcacctta
     4261 aatccacatc gctgtccata gggcaggttt tgcagctgga caaacaccaa agtacttcca
     4321 acaaaacaag cgctcgatcc acacataaaa cctcggctac cagccgcggc actaaaagcc
     4381 agtcatcttc ccgtaagaaa acaagcagta cccatagtaa aaaacacacc ttgtccaaac
     4441 attaactctt cttaaaccat gacgaatcga ctgtcctgcg agtatttctt gtacacaaga
     4501 cacgtgatgg caacagtttc aagatggatg gcgaccttgc tatgtgccgg tttggttgcc
     4561 tgcagcgccg acgcccctga tcaaagttca acagcctttg accaatccta cccagcccag
     4621 tctggcggca cacttatcag cgccatgcct tcagacccaa gtagcatgat aggtatggtt
     4681 gctggcgaat ctgcttccct ggccatcggc tcttaccttt ttaattcctt gctcaaatat
     4741 gaccagaacc tggaccttgc tggtgagctg gctgaatcct ggcaagtggc agacgacaag
     4801 aaaaccatca cgttcaagct caaaccggac ttgcagtggg cagatggcaa gccgctgacc
     4861 agtgccgata tcttatttac ctggcaacta atcaccgatg agagcacgca tagcccttat
     4921 gcctcggact accagttagt gaccaaagct gaggcgccgg atgcgcgtac atttatcgtg
     4981 cactactcac agccatttgc accggcgctt gagagttggg ctagcctgca agtgttgcct
     5041 aaacacaagc tggccggaca ggatatccgc accaccagtt ttgcgcaaaa accgctgggc
     5101 agccattact accagttaaa agaatggcgc catggtgaat atattaagtt ggaaaagaat
     5161 cctaaatctg tgttgggtcc tgcctatatt gatcacctga tcgagcgcat tattcctgac
     5221 cctgccgcac aatttctgga attgatggca gataacatcg atagcatgag tatggatccg
     5281 attacttatg ctcgaattat tccatcccgg ccagcgttag aagcacgcct gaatcagttt
     5341 aaagagttag gcaacagcta tacctacctg ggttttaacc tgaaacgtaa accgtttaat
     5401 gatgtgcgtg tacgcaaagc cattaattac gccatagaca agcaagagat tatcgatggt
     5461 gtttacctgg gtctgggcat cacgattgct tctccataca agcccggtac gcgctggagc
     5521 aatccagatc tgcagcccta ctcatttgat ccagcgaaag cgaaacagtt attgatagaa
     5581 gctggttacc aagacactga cggggatggg attgtcgacc gcgct
//
LOCUS       DATMSK010000086         4199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_54068,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000086 DATMSK010000000
VERSION     DATMSK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54068"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VNZ84_03015"
     CDS             complement(<1..759)
                     /locus_tag="VNZ84_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWU65953.1"
                     /translation="MDKAKLYTLRWSAMQIARLSADPKVDALALSQIEILSQLLEEPI
                     PEEFRPPEPPRPSLHVEEAAPAKKRYPLGEKKLWYPEALVRTDIKRAAQGEYPKGYPY
                     GAIVHFTAGQYSKGVENAVTAISSSPYFFCCIGTDGKLVQANPLNQWGYHAGESAWTI
                     DGAKRNGVSNFLVGIEITNPGRLDIDKGKYYTYWDTGRKQPIDAKKVRVITQNKDNIL
                     AGAYLPYTKEQETALIEFLLWAKANNPTVFDFDYV"
     gene            1013..1684
                     /locus_tag="VNZ84_03020"
     CDS             1013..1684
                     /locus_tag="VNZ84_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWU65954.1"
                     /translation="MIKAAIFDMDGLLIDSERIIMQGCIQAAATIGITYTQAEYVELV
                     GRVWSDSTRLMTQQLGSEANFQHVMQGLNAFLEARDHAFPLKDGVQALLQHYRSHGII
                     CSVASSSPIPHITHRLTHTGVLDYFSHVTSGQEVKRGKPHPDIYLLALEKLGLSADEC
                     IAFEDSELGAQAAIAAGLRVVVVPDLRQPSDQVRANAHQVVESLQHWLEQHDDLSGCV
                     LTRTE"
     gene            complement(1667..2305)
                     /gene="tmpT"
                     /locus_tag="VNZ84_03025"
     CDS             complement(1667..2305)
                     /gene="tmpT"
                     /locus_tag="VNZ84_03025"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HWU65955.1"
                     /translation="MHHDFWHQRWQNQQIGFHQGEVNPFLLAHFQALGLQVGARVFVP
                     LCGKSLDMHWLLAQGFQVVGVELSQLAVDALFAELGLTPEITQSGVLRCYHTRQIEIF
                     QGDFFALTQNQLGTVNAIYDRAALIALPDEMRKQYCQHLMAITQTAPQLLISFHYDQS
                     RVPGPPFSVSKAEVSANYKSHYAITELASVSLEKGMKGQYPAEETAWLLSPR"
     gene            complement(2381..3628)
                     /locus_tag="VNZ84_03030"
     CDS             complement(2381..3628)
                     /locus_tag="VNZ84_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65956.1"
                     /translation="MINSLDFARHGDKQNSAFFEEYLQKLLEERDQMGLTEMIQEIDA
                     LMITVDPDHSIQYIAELSLMTSYHYLVTLESEKHWTHILRIDLNSPDLLVREVKDPNL
                     RGIFRSLNEIYPVGARKPNSRYMGEIIRVTNMHEVVKLQEEREFRFFTQDEVRKLELP
                     GNVAVSKPSPYTHNIVGYMKREPGELRVYSLGISTIRPDVQAAYEQAKDQQAKLRIDD
                     LILPVDHLATRVYSQNREVAILEYLSWSSYFFWGAYNIWDQNSSTNVTKSAHGFAETK
                     SPAKVFTANNTPYFVNHLEKLPSPTETFVRNYGPRLHHIALSVKDGTRDGKENIDFVV
                     DAIASQGKGFLLDVIGSKEEGLKQIFSNASEHSSLIIEYVQRYDGFDGFFTRDNVAKL
                     TEAAGVEEGVLEAQAKLSQLAGQ"
     gene            complement(3807..4094)
                     /locus_tag="VNZ84_03035"
     CDS             complement(3807..4094)
                     /locus_tag="VNZ84_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65957.1"
                     /translation="MMLLCIFAVSVNAANSAPDDIQAKTADGDEVVLHSNGYWEFKDA
                     KKAEVAKIKVEEFERSNGCPLGMRPSFLGMGRCIAYDDPILKRGSLSGKGR"
BASE COUNT          967 a         1011 c         1107 g         1114 t
ORIGIN      
        1 tacatagtca aaatcaaata cggtcgggtt gttggccttg gcccagagca ggaactcaat
       61 taaagccgtt tcctgctctt tggtataagg caaatacgcg ccagctagga tgttgtcttt
      121 gttttgtgtg atcacacgaa ctttcttggc gtctatgggc tgtttacggc cagtatccca
      181 gtaggtgtaa tatttgccct tatcaatatc cagtcggccc ggattcgtga tttcaatccc
      241 taccagaaag ttggataccc cattgcgttt tgcaccatct atagtccagg cactttcccc
      301 ggcatgatag ccccactggt tgagcgggtt ggcctgcacc agtttgccat cggtgcctat
      361 acagcaaaag aaataggggc tacttgagat ggcggtgacc gcattttcaa cgcccttgga
      421 atattgtccg gcagtaaagt ggacaatggc accataaggg tagccttttg ggtattctcc
      481 ctgtgcagca cgcttgatat cagtgcgcac cagggcttcg gggtaccaca actttttttc
      541 gccgagcggg tagcgctttt ttgccggggc ggcctcttca acatgcaaac tggggcgagg
      601 tggttccggt ggcctgaact cttccgggat cggttcttcc agcagttgcg agaggatttc
      661 aatctggctg agggctagtg catctacttt tgggtcggca gaaagcctgg caatttgcat
      721 ggcagaccag cgtaaggtgt acagttttgc tttgtccatt gggggctcct ttacgattaa
      781 aacgatccag tgccgccact tgttctccca cgctgctgtg gcgatctatt catggcatgc
      841 gcatccagcg tatgctggtg tcatgtggtt ttatattagc cagcctttgt taagttgtgt
      901 tggttttgca ggtttctggt ggaaaataaa gcgacttgcg ccaaaaagtg tgtgtttagg
      961 cgcaactgag tcgcggtaaa ataatgtttt gtttgccagg caggtgtagt aggtgattaa
     1021 agcggctatt ttcgacatgg acggtctgtt gatcgactcc gagcgcatca ttatgcaggg
     1081 atgcatacag gcagcggcca ccataggcat cacttatacg caggccgagt atgtcgagct
     1141 ggtcgggcga gtctggtccg actccacacg gctgatgaca cagcaattag gtagcgaagc
     1201 caattttcag catgtcatgc aagggctcaa tgcctttctg gaggcgcgtg atcatgcttt
     1261 cccactcaaa gacggcgttc aggcattgct acagcattat cggtcacatg gcattatttg
     1321 cagtgtggcc tcttcttcgc ctatcccgca tattacacac cggctcacgc atactggcgt
     1381 actggattat ttcagccatg tcacgagcgg gcaggaggtc aagcgcggca aaccacaccc
     1441 ggatatttat ttattggcgt tagaaaaatt gggcttgtcc gcggatgaat gtattgcatt
     1501 tgaggacagc gagctgggcg cacaggcggc gattgcggcg ggtttgcgtg ttgtcgtggt
     1561 gccagacctc agacagccca gtgaccaggt acgtgccaat gcgcatcagg tggtagaaag
     1621 cttgcagcac tggctggaac agcatgatga cctatcaggc tgtgtcttaa cgcggactga
     1681 gtaaccaggc cgtttcctcc gcagggtatt gccctttcat ccctttttcc agagagacgc
     1741 tagccaattc tgttattgca tagtgtgact tgtagttggc actaacttct gctttgctca
     1801 cactaaacgg tggaccaggt acccgtgact ggtcatagtg gaaactgatc agcagttgtg
     1861 gcgccgtttg cgtgattgcc atcaggtgct ggcaatattg cttgcgcatt tcgtcaggca
     1921 gggcaatcag ggcagcccgg tcataaatgg cattcactgt gccgagctgg ttttgtgtta
     1981 aggcgaaaaa gtccccttgg aatatctcga tctgtctggt gtgatagcac ctgagaacgc
     2041 ccgattgcgt aatttcaggc gttaagccaa gttcagcaaa caaggcatcg acggccagct
     2101 gactcaattc aacaccaacc acttgaaagc cttgtgccag caaccagtgc atatccagcg
     2161 atttgccgca cagcggcaca aacacgcgcg cgccaacttg caatcccagc gcttgaaagt
     2221 gcgccagtaa aaacgggttg acttcgcctt ggtgaaagcc tatttgctgg ttctgccagc
     2281 gctggtgcca gaaatcgtga tgcatggata cgtccgctca ggagggagga ctgatagtat
     2341 aaagcagcga tgggcatccg ccgacacgag gtaaccagta ttattgacct gccagttggc
     2401 tcaacttggc ttgcgcttcg agtacgcctt cttccacgcc cgctgcttca gtgagtttgg
     2461 ccacgttgtc gcgggtgaaa aaaccatcga agccatcgta gcgttgtacg tattcgataa
     2521 tcagtgacga atgttcagag gcattggaga aaatttgttt caggccttct tccttggagc
     2581 caatcacgtc aagtaaaaag cctttacctt ggctggcaat cgcatccact acaaaatcaa
     2641 tattctcctt accatcccgc gtgccatctt tgactgatag ggcaatgtgg tgcaaacgtg
     2701 ggccataatt acgcacaaaa gtctcggtgg gagatgggag tttttctagg tgattgacga
     2761 aatagggcgt gttgttggca gtaaatactt tggccgggct cttggtctcg gcaaaaccat
     2821 gcgcactttt agtgacgttg gtggaggagt tttggtccca gatgttgtat gcaccccaga
     2881 aaaaatagct ggaccaggac aggtattcca ggatggctac ctcacgattc tggctgtaga
     2941 cacgtgtcgc caggtgatcc actggcagaa tcaggtcgtc tatgcgtagc ttggcctgct
     3001 gatctttggc ttgctcgtaa gccgcctgca catcggggcg tatggtgctg atgcccaggg
     3061 agtacacacg caactcaccg ggctcgcgtt tcatatagcc gacgatatta tgtgtgtatg
     3121 gcgacggttt gctgacggcg acattgccag gcaactccag cttgcgcact tcgtcctgcg
     3181 taaaaaagcg gaactcgcgt tcttcttgca gtttgaccac ttcgtgcata ttagtcacgc
     3241 ggataatttc acccatgtag cggctgttgg gtttgcgtgc ccccaccggg taaatttcat
     3301 tcagtgagcg aaaaataccg cgcaggttgg ggtccttgac ctcgcgcacc agcaaatcgg
     3361 gcgagttcag gtctatgcgt aaaatatgtg tccagtgctt ttcactctcc agcgtcacca
     3421 ggtagtggta tgaggtcatc agtgagagtt cagcgatata ttgaatggaa tgatccgggt
     3481 ccactgtgat catcagcgca tcaatttcct gaatcatctc ggtcaggccc atctggtcac
     3541 gctcttctag cagcttttgc agatactcct caaagaaagc cgagttctgt ttgtcgccat
     3601 ggcgtgcaaa atcgagggag ttaatcatcg tgttttaaat ttcaaaggta aagtcttgat
     3661 taagcaaacg gcgttcctga tacggtacca gtcggtggtg ctgtcaaatc gcgcgttgtg
     3721 gctggtttgc agataccaag ctagtcaatc gtccgcctta aaaagcaaca gggcaaccgc
     3781 agctgccctg ttttagtgct ttaatcttaa cggcctttac cgcttaatga gccgcgtttc
     3841 aggatagggt catcataagc aatacaacgc cccataccca aaaagctagg ccgcatgccg
     3901 agcgggcagc cattgctgcg ctcaaattct tctactttga tcttggcaac ttctgctttc
     3961 ttggcatctt taaactccca gtagccattt gaatgtagga ccacttcgtc accgtcagca
     4021 gttttggctt gaatatcgtc gggtgcgctg ttagctgcat tcactgagac agcaaaaatg
     4081 cataacaaca tcatgagtat gggtttgata agaaacatct atagacctga aaaattaaga
     4141 ttaggtaaga ctaaggtaat tgatctagaa agtacaggtt ttaaaatgag tttgtcatt
//
LOCUS       DATMSK010000087         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_194808,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000087 DATMSK010000000
VERSION     DATMSK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194808"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..640)
                     /locus_tag="VNZ84_03040"
     CDS             complement(23..640)
                     /locus_tag="VNZ84_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWU65958.1"
                     /translation="MALPILYSYRRCPYAMRARMALWAAQVQLEVREVSLRDKPAHLL
                     QISPKGTVPVLQLPDGTVLEQSLDIMQWALAQHDPQGWLHADREAVNHFISINDGDFK
                     KALDRYKYPERYPEQQQLFYRQQGEQFLQALETVLQQHRFLLSDSASVADVAIFPFIR
                     QFAAVDAEWFAGSAYPQLHAWLNGWLESALFVQIMQKFPTYIEST"
     gene            complement(640..1656)
                     /locus_tag="VNZ84_03045"
     CDS             complement(640..1656)
                     /locus_tag="VNZ84_03045"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HWU65959.1"
                     /translation="MNVYPLLKPLLFQLDAEQAHDITLQSIKRAEKLGLLKLAATRLP
                     DQPTQVMGLTFPNPVGLAAGLDKNAAVIDGMAALGFGFIEVGTITPRPQPGNPKPRLF
                     RLPEAQAIINRFGFNNLGVDQLIQNVKASKFKGILGINIGKNFDTPNDRAAEDYLTCM
                     RKVYSHASYITVNISSPNTKNLRALQEKDALGQLLATLKSEQKILSQVHGKYVPVVLK
                     IAPDLTTEQIQEIAELLMQHRMDGVIATNTTLARDAVAGLPNAEEAGGLSGAPVKAGS
                     TRVIQTLAETLQGSLPIIGVGGILNGEDAKEKMAAGASLVQLYSGLIYKGPQLIKDCR
                     KALL"
     gene            1811..1936
                     /locus_tag="VNZ84_03050"
     CDS             1811..1936
                     /locus_tag="VNZ84_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HWU65960.1"
                     /translation="MKILIDLLSTDYGLMSFIVIAIIFVVMTWAGIYVYIKARQQ"
     gene            complement(1989..>3437)
                     /locus_tag="VNZ84_03055"
     CDS             complement(1989..>3437)
                     /locus_tag="VNZ84_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWU65961.1"
                     /translation="QSAIGEIADDISDCLRCGKCKPVCSTHVPRANLLYSPRNKILGT
                     SLLIEAFLYEEQTRRGISLKHFDEFNDVADHCTVCHRCEKPCPVNIDFGDVSVKMRNF
                     LREQGKKKFNPGTAAGMMFLNVKDPATIKLLRGTMIQMGYKAINIANSLAKKFGLLKD
                     KKAPPATVGRAEIKAQVVHFINRPMPKKMQSKTSRAMMGLEDDTMIPVIRNPQKVNED
                     SEAVFYFPGCGSERLFSNVGLATQAMLYEVGAVTVLPPGYLCCGYPQTAAGNHDKGQE
                     ITADNRVLFHRVANTLNYLDIKTVIVSCGTCMDQLQKYEFEKIFPGCRLLDIHEYLME
                     KDMRLQGVTGTRYMYHDPCHTPMKTYKPLEVTNKLMGTEVALNDRCCGESGTFAAARP
                     DIATQVKFRKQEELEKGMAKMGLTVGAPEQPVKILTSCPSCLQGLSRYGDDTGIEADY
                     IVVEMAKHVLGENWMENYIQKANNGGIEKVLL"
BASE COUNT          834 a          875 c          822 g          906 t
ORIGIN      
        1 atgtccactc aggcgatact gactatgtcg actcaatata tgttggaaac ttctgcataa
       61 tctgcacaaa caacgcactt tccagccaac cgtttaacca agcatgcaat tgcggataag
      121 cactaccagc gaaccactca gcatccaccg ccgcaaactg gcggataaac ggaaaaatcg
      181 ccacatccgc tacactggca gaatcactca acaaaaaacg atgttgctgc aagactgttt
      241 ccagcgcttg caagaactgc tcgccttgct ggcggtaaaa caattgctgt tgttccggat
      301 aacgctctgg atatttatag cggtcgaggg cttttttaaa atcaccatca ttgatactaa
      361 tgaagtgatt aacggcttca cggtcagcat gtaaccagcc ttgcggatca tgctgcgcta
      421 atgcccactg cataatatcc agactttgct ctaacaccgt gccatctggc agttgcaaga
      481 ctggcaccgt gcctttgggg gagatttgca ataaatgtgc aggcttgtcg cgcaatgaca
      541 cttcacgcac ttcaagctga acttgcgctg cccataaggc catacgggca cgcatggcat
      601 atgggcaacg gcggtaggag taaagaatag gcaatgccat tagagcagtg ctttgcggca
      661 atccttgatg agttgtggac ccttataaat caggccactg taaagctgca ccaaacttgc
      721 gcctgcggcc attttttctt tggcatcttc tccattcaaa ataccaccga caccaataat
      781 cggcaggctt ccttgcagcg tctcggccag ggtctgaatc actcgcgtag aacctgcttt
      841 gacaggtgcg ccagatagtc cgcccgcttc ttctgcattg ggcaaaccgg cgacggcgtc
      901 gcgtgcgagg gtggtattcg tggcaatgac gccgtccatg cggtgctgca tgagcaactc
      961 agcaatttcc tgtatttgct cggtggttaa atctggagca attttgagca caacgggcac
     1021 atacttgcca tgcacttgcg acaggatttt ttgttcggat ttaagcgtcg ccagcaattg
     1081 gcccagcgcg tctttttcct gcagcgcgcg caggtttttg gtgttggggg aagagatatt
     1141 taccgtgata tagctggcat ggctgtacac tttacgcata caggtcaggt aatcttcagc
     1201 agccctgtca ttcggcgtat caaaattttt accaatatta atacccagaa tgcctttaaa
     1261 cttgctggct ttgacgttct gtatcagttg atccacacct agattgttaa aaccaaagcg
     1321 gttaataatg gcttgcgctt ctggcaggcg gaacaaacgc ggtttgggat tcccaggttg
     1381 tgggcgtggc gtgatggtac ccacctcaat aaagccaaaa ccgagcgctg ccatgccgtc
     1441 aatcacagct gcatttttat ccagcccagc ggcgaggccc acagggttag ggaatgtcag
     1501 ccccatcacc tgcgtaggct gatctggcaa acgcgtggct gccagcttaa gtagcccaag
     1561 cttttctgcg cgtttgatcg attgcaaggt gatatcgtgt gcttgttctg catcgagttg
     1621 aaacaacaga ggtttaagca gcgggtagac attcatactt caatttcctg aacagggttt
     1681 gcaacatcgc agcatattac aacgtttgct gcttacatca cttttgagtt acatcaaaaa
     1741 ccatattgtt aatagggttc aaaataatcc tcagatggct tgcaagatta tctgacagga
     1801 ggaacccaat atgaaaattt tgattgattt gttatctact gattatggtt tgatgagctt
     1861 tatcgtcatt gccattatct ttgtggttat gacttgggct ggcatttatg tctacatcaa
     1921 agcccgccag caataggctt taaacatgat cagtagcaca aaccccgctt acgcggggtt
     1981 tgtcttgttt aaagcagcac tttctcaatg ccgccattgt tggctttctg gatgtaattt
     2041 tccatccagt tttcaccgag cacatgcttg gccatttcga ccacaatata gtcggcctca
     2101 ataccagtgt catcaccgta acgtgacagg ccttgcaagc agcttgggca agaggtcagg
     2161 attttaactg gctgttcagg cgcacccaca gtcagaccca tcttggccat gcctttttct
     2221 agctcttcct gcttgcggaa tttgacctgg gtggcaatat caggacgtgc tgccgcaaac
     2281 gtaccagact caccacagca acggtcattc aacgcaacct ctgtccccat cagtttatta
     2341 gtcacttcca atggcttgta agtcttcatc ggcgtgtggc atggatcatg gtacatatag
     2401 cgtgtgccgg tcacgccttg caggcgcatg tctttttcca tcaggtattc gtggatatcg
     2461 agcagacggc agcccgggaa gatcttctca aactcgtatt tctgcaactg atccatacag
     2521 gtaccgcaag acacaatcac cgtcttaata tccaggtagt tcagcgtatt tgcgacgcgg
     2581 tggaacaata cacggttatc ggctgtgatc tcctgacctt tgtcatgatt accagccgca
     2641 gtttgcgggt aaccgcagca caaatagcct ggtggcaaca cagtgaccgc gccaacttcg
     2701 tacagcatcg cttgtgtcgc caaccctaca ttcgagaata aacgctcgga accacaaccc
     2761 gggaaataga acacggcttc cgagtcctca ttcacttttt gcggattacg gatgaccggg
     2821 atcatggtgt catcttccag ccccatcatg gcgcgtgagg ttttggattg cattttcttc
     2881 ggcatcgggc ggttgatgaa atgaaccacc tgcgccttga tttctgccct gcccacagtt
     2941 gcaggcggtg ctttcttgtc ttttagcaaa ccaaatttct tggccaggct gttggcgata
     3001 ttaatggctt tatagcccat ttgtatcatg gtgccgcgca acaactttat cgtggccggg
     3061 tctttgacat tgaggaacat catgccggcg gcagtgccag gattaaattt ctttttgcct
     3121 tgctcgcgca ggaaattacg catcttgacc gacacgtcac caaagtcgat attgaccggg
     3181 caaggtttct cacagcggtg gcagaccgta caatggtcgg ccacgtcgtt gaattcgtca
     3241 aaatgcttga gcgaaatgcc gcgacgagtc tgctcttcat acaggaatgc ctcgatcagg
     3301 agcgaagtgc ccagaatttt attccgtggt gagtagagca agttggcacg cggcacatgc
     3361 gttgaacaca caggcttaca tttaccgcag cgtaagcagt cagaaatatc atcggcgatc
     3421 tcgccaatgg cagactg
//
LOCUS       DATMSK010000088         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_77158,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000088 DATMSK010000000
VERSION     DATMSK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77158"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..372)
                     /locus_tag="VNZ84_03060"
     CDS             complement(76..372)
                     /locus_tag="VNZ84_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HWU65962.1"
                     /translation="MGLRAQLATLAGRFKREVLFYRALHAHPQTPWLAKALLWLALGY
                     LVLPFDLIPDFIPVIGQLDELVIIPLLLYLALKLTPVSVRQACRDRVPAEQDQK"
     gene            complement(383..1282)
                     /locus_tag="VNZ84_03065"
     CDS             complement(383..1282)
                     /locus_tag="VNZ84_03065"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWU65963.1"
                     /translation="MRAPLLETSIKSLKKLHQGKVRDIYDIDDHTMLLVSTDRLSAFD
                     VILPTGIPHKGAMLTQMANFWFEQLQDVVPNHLTGIAPESVVAPDEVEQVKGRAVVVK
                     KLKALPLEAIVRGYLVGSGWKEYTAKGTVCDILLPAGLQLAQQLPKPLFTPSSKAAVG
                     EHDENISVAQVEALIGKDLTAQVEKAAIALYTRAAEYARIKGIIIADTKFEFGVDDQG
                     VLHVMDEVLTPDSSRFWPADQYQIGSNPPSYDKQYVRDWLESCGWNKTPPGPELPADV
                     AQRTSDKYMEAFAKLTGRTLLAE"
     gene            complement(1387..2442)
                     /gene="gap"
                     /locus_tag="VNZ84_03070"
     CDS             complement(1387..2442)
                     /gene="gap"
                     /locus_tag="VNZ84_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWU65964.1"
                     /translation="MGIRVGINGFGRIGRMVLRAALNQAEFKDMEVVAINGIEEPDHM
                     LYMLKYDSVHGRLAQDAHLEGGFLVIDGKKIRLTAHAKPADIDWKSAQVDVVVECTGV
                     FLTQETCAAHIAGGARIVVQSAPGKDDTPMFVYGVNHYHYRGEDIVSAASCTTNGLAP
                     IVKVLHDNFGVKRGLMTTIHAATATQKTVDGTSNKDWRGGRGVFDNIIPSSTGAAKAV
                     GKVIPALNKKLTGMSMRIPSADVSVVDLTVELQQKTTYEEICAVMQYAAERDLKGVLG
                     YTNEAVVSSDFRGYPAASVFDAGAGIMLDPTFVKLIAWYDNEYGYTCNLLRLTRYVGY
                     AKLSMTDLPEEDEAVAA"
BASE COUNT          673 a          718 c          584 g          623 t
ORIGIN      
        1 acatcttctc aaacccatct caacaaaaaa tcgccattat aggcgaataa tatgaacatt
       61 tgcttgcaaa tagcactatt tttgatcttg ctcagcaggc actctatcgc ggcatgcctg
      121 cctgacactg actggggtca acttaagcgc caggtaaagc aacagtggaa taatgaccag
      181 ctcatccagc tggccaatca caggaataaa gtccgggatc aggtcaaatg gcaacaccag
      241 gtatcctaac gccagccaga gcaacgcttt ggcgagccat ggtgtttggg ggtgcgcgtg
      301 caatgcacgg taaaacaaaa cctctctttt aaaccggcca gccaatgtcg ccagttgcgc
      361 acgcaatccc atgtcagtgc aattactctg ccagcaaagt acggccggtc agcttggcaa
      421 acgcttccat atatttgtca gaagtgcgtt gcgccacatc cgctggcaac tcagggccag
      481 gtggcgtctt gttccaacca caactttcca gccagtcacg tacatattgc ttatcataac
      541 tgggcgggtt gctgccaatc tggtattggt ctgcaggcca gaaacgtgaa gagtccgggg
      601 tcagcacctc atccatcaca tgtaacacgc cctgatcatc gacaccaaat tcaaacttgg
      661 tatcggcaat gataataccc ttgatacgcg catattcggc agcgcgcgta tacaaagcaa
      721 tggcggcttt ttcgacttgt gctgttaggt ctttgcctat cagcgcctcc acttgggcga
      781 cgctgatatt ctcgtcatgc tcgccaacgg ctgctttgct tgaaggcgta aacaacggtt
      841 tgggtaattg ttgcgccaat tgcagtccgg ctggcaatag aatatcgcag accgtgccct
      901 tggccgtgta ctctttccag ccactaccga caagatagcc acgcacaatc gcctcaagcg
      961 gcaaggcctt gagctttttc accaccacgg cacggccttt gacctgctca acctcatcgg
     1021 gtgcaaccac agactcaggg gcaattccag ttaaatgatt gggaacgaca tcttgcaatt
     1081 gctcgaacca aaagttcgcc atttgcgtga gcatggcacc cttatgcggg atgcccgttg
     1141 gcaaaatcac atcaaaggcg cttaaccggt cagtactcac cagcaacatg gtgtggtcat
     1201 cgatatcgta aatatcgcgc accttaccct ggtgcaattt tttcaggctt ttgatggaag
     1261 tttcgagcaa aggtgcacgc ataatgtcta tcacacaagt aaaagcagca ttataaaggc
     1321 gtgacacgat tgctggcgaa taaattgtct atcgttactc cccgcaggat tttatcctgc
     1381 aagctctcaa gcagcgacgg cctcatcttc ctcaggcaag tctgtcattg ataatttggc
     1441 atagccaaca taccttgtta accgtaacag attacaggta tagccatact cattgtcata
     1501 ccaggcgatc aatttgacaa aggtcgggtc cagcatgata ccggcccccg catcaaacac
     1561 actggccgcc ggataacccc tgaaatcgct ggaaacaacc gcttcattgg tataccccag
     1621 aacacctttc agatcgcgtt cggcggcgta ttgcatgaca gcacaaatct cttcatacgt
     1681 cgttttttgt tgcaactcaa ccgtcaagtc aaccacagac acatctgctg aaggaatgcg
     1741 catggacatg cctgtcagct ttttattcaa ggcaggaatc actttaccca ccgctttggc
     1801 cgcgccggta ctggaaggaa taatgttatc gaagacaccg cgacccccac gccaatcttt
     1861 attggaggta ccgtctacgg ttttttgggt agcagtagcc gcatggatcg tcgtcatcaa
     1921 accgcgtttg acgccaaaat tgtcatgcag cactttaacg ataggggcca agccgttagt
     1981 cgtacaagac gctgcagaga cgatatcttc accacggtag tgataatgat tgacgccata
     2041 gacaaacatg ggcgtatcgt ccttgccggg cgcggactgg accacgatgc gtgcaccacc
     2101 tgcaatatgg gcagcacacg tttcctgcgt cagaaacaca ccggtacact caaccacaac
     2161 atctacctgt gcagatttcc agtcgatatc cgccggtttg gcatgcgcag tgaggcggat
     2221 ttttttacca tcaatcacca gaaaaccgcc ctctaaatgt gcatcctgcg ccaacctacc
     2281 gtgcaccgag tcatacttga gcatgtacag catatggtca ggctcctcga tgccgttaat
     2341 ggccaccacc tccatatcct taaactcagc ctgatttaat gcggcgcgca gcaccatccg
     2401 gccaatgcgg ccaaacccgt taatgcctac tcgaatgccc atttttgact ccttcgacac
     2461 gattagccaa aagataagca attttaatgc taatttacat tccaaagatt agacaggcag
     2521 tgcgcgcacg tctagaatag cttgagcaag cctttcctgt tgtgggttta actagctacc
     2581 ggatattttg tggacatc
//
LOCUS       DATMSK010000089         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_7072, whole
            genome shotgun sequence.
ACCESSION   DATMSK010000089 DATMSK010000000
VERSION     DATMSK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7072"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..2117
                     /gene="uvrC"
                     /locus_tag="VNZ84_03075"
     CDS             312..2117
                     /gene="uvrC"
                     /locus_tag="VNZ84_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWU65965.1"
                     /translation="MSMFDPKPILKNLPNLPGVYRMLNAEDTVIYVGKAKDLKKRVSS
                     YFNKNLASPRTKMMVSQIANIETTVTRSEAEALLLENNLIKGLMPRYNVLFRDDKSYP
                     YIALTGDAFPRLAFHRGAQRKGSQYFGPFPSSPAVRESIQLLQKVFKLRTCENTVFSN
                     RSRPCLQYQIARCTAPCVNLISQEDYAGDVRHAAMFLLGKTSEVMDSLGDAMNHAAEE
                     MEYEQAAVLRDRIQALRQVQAKQFVSDFSVSDADVIACAEVQGQHCINLVMIRGGRHL
                     GDKSFFPKNSQDADVAETMEAFITQYYLSQNTPPLLVCGAEVDKAEFEDMLSEQSGRK
                     IRVLTNAIGDKKVWLKMAQTNAELALQQRQATSANQQARLLALREALNLSEGTERIEC
                     FDISHTMGEATVGSCVVFDRGDMQNSEYRRYNVTGITPGDDYAAMRDVLTRRYRKVAA
                     GEGKRPDLIFIDGGKGQLGVAIEVMQEVGLDDILLVGIAKGEERKPGLETMIFSDTGE
                     MLNLPMDNPGLHLLQQIRDEAHRFAITGHRAKRAKARITSSLEEIEGVGAKRRKALLT
                     RFGGLDAIKSASIDEIAQVEGISLALAQTIYERLH"
     gene            2146..2727
                     /gene="pgsA"
                     /locus_tag="VNZ84_03080"
     CDS             2146..2727
                     /gene="pgsA"
                     /locus_tag="VNZ84_03080"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWU65966.1"
                     /translation="MWNIPNILTLLRIGMIPVFVGIFYLPANAVTPDAVPAHWVNLTA
                     AGVFILAALTDWLDGYWARKYHQMSKFGAFLDPVADKLMVAAALIVLVELHRVGAVVA
                     LIIIGREIAISALREWMASIGKSANVAVAMVGKVKTAAQMVAIILLLWYDPLFGLDIQ
                     WLGEWLIVIAALLTLVSMGYYLRAAWPAIRETL"
BASE COUNT          685 a          645 c          778 g          708 t
ORIGIN      
        1 gggtccgtgg gcgaggctac ggctacaagc tcgtcgatac cttagcttta tatttgaagt
       61 cggagggata cacaggtgtt gggccgatcg atatacaaac ccatgacgaa gcatcccgca
      121 ttgcgtctgg aaaacttgca gatcgctttg caaggcattt tggtcataag taatacgctt
      181 tatcccgagt tgcagttgac gaactacaat aaagtttgcc ctctcgtctt gatggtcgca
      241 aatagcatgc attctctaaa ggtatgcatt catcagtgta taaacctgtg ggcatcgctc
      301 gatgctaaaa tatgagcatg ttcgacccca agcccattct caaaaaccta cccaacctgc
      361 ctggtgttta ccgcatgctc aatgcggaag atactgtaat ttatgtaggc aaagccaaag
      421 acctgaaaaa gcgggtttcc agttacttta ataaaaatct tgccagtccg cgcaccaaga
      481 tgatggtgtc gcagattgcc aatattgaga ccaccgtgac gcgctcggag gctgaagcgt
      541 tgttgctgga aaataacctg atcaaaggct taatgccgcg ttacaacgtg ttgttccgcg
      601 atgacaagtc atacccttat attgcgctga ctggcgatgc gtttccccgc ctggcttttc
      661 atcgtggggc acagcgcaag ggctcgcagt attttgggcc gtttcctagc tcgcctgcgg
      721 tgcgcgagag catacagttg ctgcaaaagg tgtttaagct gcgtacctgt gaaaataccg
      781 tgttttccaa ccggtcgcgg ccttgcctgc aataccagat tgcgcgttgt accgcgcctt
      841 gtgtcaacct gatttcacag gaagactatg ccggtgatgt acgccatgcg gcgatgtttt
      901 tattgggtaa aaccagtgag gtgatggact cactgggcga tgccatgaat catgcagcag
      961 aagaaatgga gtacgagcaa gcggctgtgc tgcgtgatcg cattcaggcg ttgcgccagg
     1021 tgcaggccaa gcagtttgtg agcgatttca gcgtcagcga tgccgatgtg attgcctgtg
     1081 cagaggtgca gggccagcat tgtattaatc tggtgatgat ccgtggtggc cggcatttgg
     1141 gtgataagag ctttttccca aaaaacagcc aggatgcaga tgtggccgaa accatggagg
     1201 cttttattac ccagtactat ttgtcacaaa atacgccgcc attgctggtg tgtggcgctg
     1261 aggtcgataa agccgagttt gaagatatgc tgagtgagca gtcagggcgc aaaatccggg
     1321 tactgactaa tgcgattggc gataaaaagg tctggctcaa aatggcgcaa acgaatgccg
     1381 agttggcgtt acagcagcgt caagccactt cagccaatca gcaggcacgg ttgctggcct
     1441 tgcgtgaagc actgaatctt tcagaaggta cggagcgtat tgagtgtttt gatatcagcc
     1501 ataccatggg tgaggccacg gttggtagct gcgtagtctt tgaccgtggc gatatgcaaa
     1561 acagcgaata ccgccgctat aacgtgaccg gcattacccc tggcgatgat tatgctgcaa
     1621 tgcgggatgt attaacgcgc cgctatagaa aagtcgccgc aggtgagggc aagcggccag
     1681 acctgatttt tattgatggt ggtaaaggcc agttaggtgt ggcgattgag gtcatgcagg
     1741 aagtgggctt ggacgatatc ttgctcgtcg gcatcgccaa aggtgaggaa cgcaaacctg
     1801 gtttagaaac catgatcttt tccgacacgg gtgaaatgct gaatttaccc atggataacc
     1861 ccggcctgca tttattgcaa caaatccgcg atgaggcaca ccggtttgcc attaccgggc
     1921 atagagccaa gcgtgcgaag gccaggatta cctccagcct ggaagagatc gaaggcgtcg
     1981 gcgccaagcg tcgcaaggcc ttactcacgc gttttggtgg cctggatgcg ataaaaagtg
     2041 ctagcataga cgagattgcc caagtggaag gcattagtct ggcactggcg caaacgattt
     2101 atgaacgctt gcattgagtc tttagtattc agccatcaaa ataacatgtg gaatatcccc
     2161 aatatactta ccctgttgcg catcggcatg attccggtgt ttgtcggcat cttttacctg
     2221 ccagcaaatg ccgtcactcc tgatgcggtg cccgcacact gggtgaattt aaccgcagcc
     2281 ggagtgttta tcctggcagc actgacggac tggctagatg gatactgggc gcgtaaatat
     2341 caccaaatgt ccaagttcgg tgcttttctt gaccccgttg cagacaagct gatggtggca
     2401 gctgcactga ttgtgctggt tgaactacat cgtgtgggtg ccgtggtggc cttgattatc
     2461 atcggccgtg aaattgccat cagtgccttg cgtgaatgga tggcaagcat cggtaaaagc
     2521 gcaaatgtag cggtggcaat ggtgggaaag gtgaaaacag ctgcgcaaat ggtggcgatt
     2581 attctgttgc tctggtacga cccattattc gggttggaca tacaatggct gggtgaatgg
     2641 ttgattgtga ttgccgcttt gctgactctg gtttccatgg gttattactt gcgtgcagcg
     2701 tggccagcga tacgcgaaac cctctaaagg gctcatctaa aaagtcaatt tgcgttgcga
     2761 tactgcgtta ctcataaaaa atttctcctg acatattact tatatgctgc gtcaaa
//
LOCUS       DATMSK010000090        11762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_54121,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000090 DATMSK010000000
VERSION     DATMSK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 11762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11762
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54121"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VNZ84_03085"
     CDS             <1..523
                     /locus_tag="VNZ84_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWU65967.1"
                     /translation="YYGTGNYFGAKDILAIGVAGRYKKDGSVSPTGTVGDYKSYSVDF
                     LLEKKDVGPGTFSLEAAYYHYDTDNVFRGEQGKAYSAGLGYLFQEQIGWGKFMPIIRY
                     QKFDADGTTSYNSTTTVFTNTAGSGSSKRFEVGTNYVIAPYNATLTATVGKQDTPTTS
                     SDNFVKVALQMQF"
     gene            565..1788
                     /gene="fmdA"
                     /locus_tag="VNZ84_03090"
     CDS             565..1788
                     /gene="fmdA"
                     /locus_tag="VNZ84_03090"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HWU65968.1"
                     /translation="MKTLVKLDLDKKPWEQDGQIHNRWHPDLPMIAMVKPGDEFRVEC
                     MDWTGGQIGNNDSANDIRDVDLTQVHYLSGPIGVEGAEPGDLMVVDILDVGTFEDQQW
                     GFNGLFAKENGGGFLTDHFPEASKSIWDFHGVYTTSRHVPKVRYAGIMHPGLIGCLPS
                     RELLEKWNEREAALIATDPDRVPALALPPEPQSAVMGKLTGDAAKKAAMEGARTVPPR
                     DHGGNCDIKNLTKGSKVYFPVYVKDGGLSMGDLHFSQGDGEITFCGAIEMAGYLDIKV
                     SLIKDGVKKYGIKNPIFQPSPITPTYRDYVIFEGISVDEAGKQHYLDVHVAYRQACLN
                     AIEYLKKFGYSGEQAVAILGTAPVEGHISGIVDIPNACATLWIPTEIFEFDIRPNADG
                     PKIMVPPGIDVAPST"
     gene            1843..2181
                     /locus_tag="VNZ84_03095"
     CDS             1843..2181
                     /locus_tag="VNZ84_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWU65969.1"
                     /translation="MPLYEYECDSCGVFTALRKMSESSLPAECDCCGATSPRILSVPK
                     LAIMDKQQRSAHERNEKSAHEPRSARRSSCGCTGSHTCKTTTKVNEETGKLPLQMQTK
                     KTARPWMIGH"
     gene            2291..3529
                     /gene="urtA"
                     /locus_tag="VNZ84_03100"
     CDS             2291..3529
                     /gene="urtA"
                     /locus_tag="VNZ84_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HWU65970.1"
                     /translation="MSTLNRRGFMKGALLGAMMAAGLISGHALAADYPTAKVNTTGLA
                     VTDTTVKVGILHSATGTMAISETGSIQAEKLAIEQINAMGGILGRKIEVIQEDGASDW
                     PTFAEKAKKLLEKDKVASVMGCWTSASRKAALPVFEKDNGLLFYPTFYEGLEQSKNVF
                     YTGQEATQQILAGLDWIAKEKKAKTFYLIGSDYIWPRTSMKIARKHIEQHLHGTVVGE
                     EYIALGDTQFGSVINKIKLKKPDVIYAAVVGGSNVAWFKQLNAAGLNSSKQTMLTISV
                     TEDEVLGIGGENLAGFYSAMKYFQSQDNPENKKFVEAFKKRWGKDAVIGDVTQAAYLG
                     PWLYKAAVERAGSFDVDKVQAALPGYTFKDAPEGPVTVEANHHLTTKLRIGQWGKDGQ
                     AKVVYTSDYIKPDPFPKGYQ"
     gene            3668..4585
                     /gene="urtB"
                     /locus_tag="VNZ84_03105"
     CDS             3668..4585
                     /gene="urtB"
                     /locus_tag="VNZ84_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HWU65971.1"
                     /translation="MFGYSMADIGNIVVMQGFSGLSMFTVLLLMALGLAIIFGQMGVI
                     NMAHGEFMTIGAYTTVLISKVAANYGFTDYYFVFAIAIAFLLAFAVGYFVEFIMIRHL
                     YKRPLDTLLATWGLSLVMQQIFRSSFGAKEVSAELPEWMLGSFKPTPDIDIPINGVFV
                     MVLAALVTFGVYMFMYKSRWGLRVRATVQNRTMAGAVGINTAKVDRITFALGCGIAGI
                     AGAAFTTIASTGPTTGTLYIVDSFMVVVFGGAASIVGTIASAFGIAQAQSVLQFFMTS
                     SMGKVSTLLVIVSILMMKPEGLFASKLRK"
     gene            4831..6114
                     /gene="urtC"
                     /locus_tag="VNZ84_03110"
     CDS             4831..6114
                     /gene="urtC"
                     /locus_tag="VNZ84_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HWU65972.1"
                     /translation="MTLWEKMKASPVLGGKEGVIGLAVLAAVIFVILPLSVDIFRLNL
                     IGKYLTYAFVAVGLVMLWGHGGILSLGQGIFFGLGGYAMAMFLKLEASDPLSTKIQST
                     PGIPDFMDWNQLTALPWFWEPFKSLPFTIFAVLTIPALFAYIIGYAMFKRRVGGVYFS
                     IITQAIALILSILIVGQQGYTGGVNGMTDLRTMLGWDIRTDSAKYILYFVNGLLLFGA
                     IFLSKYIMSSKFGMLLLAMRDKEERVRFSGYDVSNFKIFIFCLAAMMSAVGGAMFTLQ
                     VGFMSPSFVGIVPSIEMVIFCAVGGRASLIGAVYGTLLVNYGKTVFSETYPELWLFLM
                     GGLFISVVMFFPNGLAGIWDKLSTKYGWFKDKAEVAKKPEVSTSGVEAQGVLTETTET
                     MLAADSTDAATKAPRKRASKKDTGLESSKMRGAEA"
     gene            6111..6854
                     /gene="urtD"
                     /locus_tag="VNZ84_03115"
     CDS             6111..6854
                     /gene="urtD"
                     /locus_tag="VNZ84_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HWU65973.1"
                     /translation="MSNTDFVLAVEDLTVSFDGFKAVDSLTMYIDKNELRVLVGPNGA
                     GKTTVLDLICGKTKSTAGSIKFNNTELTKLSEHEIVRSGVGRKFQNPSIYENLTVYQN
                     LEVSYPKGRGVFGSLTFKRTQEVEDRVRKVAADIMLTEFLDMESALLSHGQKQWLEIG
                     MLLMQEPELLMLDEPVAGMSAKERDQTADLLNRICQNRSVIVIEHDMEFVKKIAHKVT
                     VLHQGKILAEGPMEQVQADEKVIEVYLGH"
     gene            6885..7574
                     /gene="urtE"
                     /locus_tag="VNZ84_03120"
     CDS             6885..7574
                     /gene="urtE"
                     /locus_tag="VNZ84_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HWU65974.1"
                     /translation="MFEIENLQVSYGQSQVIHGLNFTANKNETLAIMGRNGMGKTTLF
                     KSLMGILPSKATKATVDGVDLQGSATHDRVMKGLAYVPQGRWIFPNMTVQENIETGLE
                     KSATREIPDDIYALFPVLFDMRKRKGGNLSGGQQQQLAIARALVTNPKVLLLDEPTEG
                     IQPSIIKDIAKVLNEIRKMREITIIVSEQVLSFTMEVADRIIVIDKGRFIHEDTRDNV
                     DAAKVKSYLSV"
     gene            7619..8485
                     /locus_tag="VNZ84_03125"
     CDS             7619..8485
                     /locus_tag="VNZ84_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWU65975.1"
                     /translation="MDDLNPAISSPHQRIAVKHWQANLELGFARRDTRSYLAHRLHKG
                     PLVVQKSLHPEGDDVCHAVVVHPPGGVAGGDALHLQVKVGEGAKALLTTPGAGKWYKA
                     NGAIATQKLTFQVEAGSGLEWLPQENILFDAAEVGFSAEIDLAADATLAAWDIVCLGR
                     QAQQEVWQQGLYRLNQRILRNSRLIWNERAYMQADDLIKKSRVGLGGAAVFGTFLVCA
                     GAVPAELLQACRQIAVSDANTGITALPDVLCIRYVGNASQQARQYFEQCWQILRPWYY
                     QKDAVRPRIWNT"
     gene            8522..8824
                     /gene="ureA"
                     /locus_tag="VNZ84_03130"
     CDS             8522..8824
                     /gene="ureA"
                     /locus_tag="VNZ84_03130"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HWU65976.1"
                     /translation="MELTPREKDKLLIFTAALLAERRKARGLKLNYPEAVAFISAAIM
                     EGARDGKTVAELMSYGTTLLARDEVMEGVPEMIPDIQIEATFPDGTKLVTVHHPIP"
     gene            8978..9286
                     /locus_tag="VNZ84_03135"
     CDS             8978..9286
                     /locus_tag="VNZ84_03135"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HWU65977.1"
                     /translation="MIPGEMQVAAGDIELNTGRETVTLDVANKGDRPIQVGSHYHFYE
                     TNDALSFDRALAYGCRLNIAAGTAVRFEPGQTRTVQLVKLAGARKVYGFAGRVMGGLK
                     "
     gene            9283..10986
                     /gene="ureC"
                     /locus_tag="VNZ84_03140"
     CDS             9283..10986
                     /gene="ureC"
                     /locus_tag="VNZ84_03140"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWU65978.1"
                     /translation="MTLKMDKHAYAEMFGPTVGDKVRLADTDLWVEVEKDFTIYGEEV
                     KFGGGKVIRDGMGQGQGLAAEVADTVITNALIIDHWGIVKADIGIKGGYISAIGKAGN
                     PDIQPGVTIAIGAGTEVIAGEGMIITAGGIDTHIHFICPQQIEEALMSGVTTMVGGGT
                     GPATGTFATTCTPGPWHIHRMLQSADAFPMNLGFLGKGNASLPEPLREQVKAGVIGLK
                     LHEDWGTTPAAIDNCLSVADEMDIQVAIHSDTLNESGFVETTIGAFKGRTIHTFHTEG
                     AGGGHAPDIIKAASHPNVLPSSTNPTRPFTVNTIDEHLDMLMVCHHLDPAIAEDIAFA
                     ESRIRRETIAAEDIFHDLGAFSMMSSDSQAMGRVGEVIIRTWQTAHKMKVQRGPLAED
                     AAGNDNFRIKRYIAKYTINPALTHGISHVVGSVEVGKLADLVVWKPAFFGVKPFTIIK
                     GGMIAAAAMGDPNASIPTPQPVHYRPMFGAYGGGLKTAVTFVSQAALESPEIKALGLQ
                     KPLVAVKGTRDVKKSDMVHNTWMPKIDVDPETYEVLADGMPLICEPAEVLPMAQRYFL
                     F"
     gene            10989..11483
                     /gene="ureE"
                     /locus_tag="VNZ84_03145"
     CDS             10989..11483
                     /gene="ureE"
                     /locus_tag="VNZ84_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HWU65979.1"
                     /translation="MIHLTERITVCGHIDDQLELPFDQRQKSRLRVTLASGKEAALFL
                     SRGIILRGGDLIQSEDGSVVVQIVAAQEPVYNVIAPTPPDLMRAAYHLGNRHVPLQIG
                     DGWLRLEQDYVLRDMLLGLGMQVSEVQAPFEPEAGAYGGGHRHGHDDDALQSLRPRLK
                     GQGH"
     gene            11483..>11762
                     /locus_tag="VNZ84_03150"
     CDS             11483..>11762
                     /locus_tag="VNZ84_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HWU65980.1"
                     /translation="MANALALSRLLQLASPLLPVGAYGYSQGLEWAIESGEVKDGASA
                     QAWIGDSLQVYQARFELPVLYKLYQAWQAGDLAQVKEWDSFYQAGRDTS"
BASE COUNT         2946 a         2717 c         3239 g         2860 t
ORIGIN      
        1 ttactacggt accggtaatt actttggtgc aaaagacatc ctggctattg gcgttgctgg
       61 ccgttacaaa aaagacggtt cagtgagccc gacaggcact gtgggtgatt acaaatccta
      121 cagcgttgac ttcctgttgg agaagaaaga tgtcggtcct ggcactttct cacttgaagc
      181 agcttactac cactacgata cagacaatgt ctttagaggc gagcaaggta aagcttactc
      241 tgctggcttg ggctacttgt tccaggaaca aattggctgg ggcaagttta tgccgattat
      301 tcgttatcag aaatttgatg ctgatggtac taccagctac aacagcacaa caaccgtatt
      361 tactaatacg gcaggaagtg gcagcagcaa gcgtttcgag gttggtacta actacgtcat
      421 cgcgccttac aacgcgacat taacagccac tgttggtaag caagacacac cgactacatc
      481 tagcgataac ttcgtcaaag tcgctttgca gatgcagttc taatcaacac cttataacca
      541 tttctcacaa aggagatgag tgtaatgaaa acactagtga agttggattt ggataaaaaa
      601 ccctgggaac aggatggcca gattcataac cgttggcatc ctgatttacc gatgatcgcc
      661 atggtgaaac caggggatga attccgcgtc gagtgtatgg actggaccgg tggccaaatt
      721 ggtaacaacg acagcgcaaa cgatatccgt gatgtggatc tgactcaggt gcactacctg
      781 agcggcccga tcggtgttga aggtgctgag ccgggtgacc tgatggtcgt agacatcctg
      841 gacgtaggga cgtttgaaga ccagcaatgg ggctttaacg gcctgtttgc aaaagaaaat
      901 ggtggtggtt tcctgactga ccatttccct gaagcaagca agtctatctg ggacttccac
      961 ggggtttaca cgacctcccg ccatgtacca aaagtacgtt atgccggcat tatgcacccg
     1021 ggtttgatcg gttgcctgcc atcacgtgaa ttgctggaaa agtggaatga gcgtgaagcg
     1081 gcgctgattg ccactgatcc tgaccgcgtg ccggcactgg cattgccacc tgagccacag
     1141 tctgccgtga tgggcaagct gacaggtgac gctgccaaga aagctgcgat ggaaggtgca
     1201 cgtacggtgc caccacgtga tcacggtggt aactgcgaca tcaaaaacct gaccaaaggc
     1261 tccaaagtgt acttcccggt ctacgtgaag gatggcggtc tctccatggg tgacctgcac
     1321 ttctcacaag gcgacggtga aatcaccttc tgcggtgcga ttgaaatggc cgggtacctc
     1381 gatatcaagg tcagcctgat taaagacggt gtgaagaaat acggtatcaa gaacccaatt
     1441 ttccaaccaa gcccgatcac tccaacatat cgcgattacg tgatttttga agggatttca
     1501 gtggatgaag ctggcaagca acattacctg gatgtgcacg tagcttaccg ccaggcttgt
     1561 ctgaacgcga ttgagtacct gaagaaattt ggttacagcg gcgagcaagc cgttgcgatt
     1621 ctgggcacgg caccggtaga agggcatatc agcggcatcg ttgatatccc gaacgcctgt
     1681 gccaccttgt ggatccctac cgagatcttc gagtttgata tccgtccgaa tgctgacggg
     1741 ccgaaaatca tggtgccgcc tggaattgat gtcgcaccaa gcacttaatt ggatggttta
     1801 acggcaagtt ttaaaggtca agtttgcaag ggaagagtac aaatgcctct ttacgaatat
     1861 gagtgtgaca gttgtggcgt gtttacggcg ctacgcaaaa tgagtgaatc cagtttgcca
     1921 gctgagtgcg attgttgtgg tgctaccagc ccacgcatcc tgtcagtgcc taagctggcc
     1981 atcatggata agcagcagcg gtccgcccat gaacgtaacg agaagtctgc acatgaacct
     2041 cgcagtgccc gtcgctccag ttgtggttgt acaggctcac atacctgtaa aaccaccacc
     2101 aaggtgaatg aggagaccgg caagttgccg ttgcagatgc aaaccaagaa aacagcaaga
     2161 ccgtggatga tcgggcacta atctgatcga ccagacaaag gttgatcggg cattcgcccg
     2221 gccggacacg gaaatgaagt aacgaacaat tgttgtattt aatcgattta tccataaagg
     2281 agaagatgta atgagtacct taaacagacg aggttttatg aaaggcgcat tgctaggtgc
     2341 catgatggcc gcgggcctca tcagcggtca cgctttggca gcagattacc cgacagccaa
     2401 agtaaacacg acagggttgg ctgtgaccga cactaccgtg aaagtaggta ttctgcactc
     2461 cgcgacaggg accatggcga tcagtgaaac aggttctatc caggctgaaa aactggcgat
     2521 tgagcagatc aacgccatgg gcgggattct gggccgcaaa attgaagtga ttcaggaaga
     2581 tggtgcttct gactggccaa cctttgctga aaaggcgaaa aaactgctgg aaaaagacaa
     2641 agtggcttct gtgatgggtt gctggacatc cgcttcacgt aaagccgctc taccagtgtt
     2701 tgaaaaagac aacggtttgc tgttctaccc aaccttctat gaaggtttgg agcaatccaa
     2761 aaacgtattc tatacaggcc aggaagcgac acaacagatc ctggcaggtc tggactggat
     2821 cgctaaagag aagaaagcta aaactttcta cctgatcggt tctgactaca tctggccacg
     2881 tacttccatg aagatcgccc gtaaacacat cgagcaacac ttgcatggca cagtggtggg
     2941 tgaagagtac attgctttgg gtgataccca gtttggttct gtgatcaaca agatcaaact
     3001 gaaaaaacct gacgtgatct acgcagcggt ggtaggcggt agtaacgtgg catggttcaa
     3061 acagttgaac gcggctggtt tgaactctag caagcaaacc atgctgacga tctcagtgac
     3121 tgaagatgaa gtattgggta tcggcggtga aaacctggct ggtttctact cagcaatgaa
     3181 atacttccaa tctcaagaca atcctgagaa caagaaattt gttgaggcat tcaaaaaacg
     3241 ctggggtaaa gatgctgtga ttggcgacgt aacccaagcg gcttacctgg gcccatggtt
     3301 gtacaaagct gcggttgaac gtgcaggtag cttcgacgta gataaagtac aagcggcatt
     3361 gccaggctac accttcaaag atgcgccaga aggtcctgtg actgtagaag ccaaccacca
     3421 cctgactacc aagctgcgta ttggtcaatg gggtaaagat ggtcaagcga aagtggttta
     3481 cacctctgac tacatcaaac ctgatccatt tccaaaaggc taccaataag atcgtagtgt
     3541 cttgaaatgc tagagcggta gccggtttca ccggctaccg tttggcgcaa gcagtaaaaa
     3601 ttctttgtag taaagattcc ctagtagtaa aaatttcata gagaagtgat gttttaagga
     3661 gaccgatatg tttggttatt cgatggcaga tataggcaat attgtggtga tgcagggctt
     3721 ttcgggcctg agcatgttta cagtgttgct gctgatggca cttggtctcg cgattatttt
     3781 cgggcagatg ggtgtgatta acatggcgca tggcgagttt atgaccatag gtgcctacac
     3841 caccgtactc atttcaaaag tagcagccaa ctacggcttc acggattact acttcgtgtt
     3901 tgccattgcg attgccttct tgctggcctt cgcggtcggt tactttgttg aattcattat
     3961 gatcaggcac ttgtacaagc ggccgctgga taccctgctg gcgacttggg gcttaagcct
     4021 ggtcatgcag caaattttcc gctcctcatt cggtgccaaa gaggtgagtg ctgaactgcc
     4081 agagtggatg ctgggttctt tcaagccgac acctgatatt gatatcccga tcaacggcgt
     4141 gtttgtgatg gtgctggcgg cgctggtgac ctttggcgtg tatatgttta tgtacaaatc
     4201 acgctgggga ttgcgcgtgc gtgcaacagt acaaaaccgc accatggcag gcgctgtcgg
     4261 gatcaatacc gccaaggttg accgtattac gtttgcgtta ggttgcggta ttgccggtat
     4321 cgcgggcgct gcgtttacga ccattgcctc cacaggccca accacaggta ccttgtacat
     4381 tgtggacagc tttatggtgg tggtgtttgg tggtgcagcc agcatcgtcg gtacgatcgc
     4441 ttccgctttc ggcattgccc aggcgcaatc tgtactgcaa ttctttatga ccagctccat
     4501 gggtaaggtg tctaccttgc tcgtcatcgt cagcatcctg atgatgaaac cggaaggtct
     4561 gtttgcctcc aaactgcgta aataaacggt ctgaaattaa acgactatca acggtaggaa
     4621 cattgagtaa atgagcgagc gggataagtt gcatggcgca cggcgcgcag aaaccgtaat
     4681 gtacaggcag tacatgagga tttcgagcac cgcgcaacgc agcaatttgc ccgcgcagcc
     4741 atttaatcaa agtttctata agtaaataga cgtattaata ctttgtacct gacgccctag
     4801 aatcaacgca tcaacagagg agaaatcttg atgacattat gggaaaaaat gaaagccagt
     4861 cctgtactgg gtggtaagga gggagtgatc ggcctggcag tgcttgccgc cgtgatcttc
     4921 gtgatcttgc cgttaagcgt agatattttc cgcctgaacc tgatcggtaa gtacctgact
     4981 tacgcgttcg tcgcagtggg actcgtgatg ctgtggggtc acggtggtat cttgagcctg
     5041 ggccaaggga ttttcttcgg tctcggtgga tatgcgatgg ccatgttcct caaactggaa
     5101 gcctctgatc ctttaagtac caagattcag tctacaccag gcattcctga ctttatggac
     5161 tggaaccagc tcaccgcctt gccatggttc tgggagccgt tcaagagctt gcccttcacc
     5221 atcttcgccg tactgacgat tccagcgctg tttgcctaca tcatcggtta cgcgatgttt
     5281 aaacgtcgtg tgggtggtgt gtacttctcc atcattacac aagcgattgc attgatcctg
     5341 agtattctga ttgttgggca acaaggctac accggtggcg tgaacggcat gactgacttg
     5401 cgtaccatgc tgggatggga tatccgtacc gactctgcca aatacatcct gtactttgtg
     5461 aatggcctgt tgctgttcgg cgcgattttc ctgtccaaat acatcatgag cagcaagttc
     5521 ggcatgttgt tgctggcgat gcgcgacaaa gaagagcgtg tgcgtttctc tggctatgac
     5581 gtttccaatt tcaaaatctt tatcttctgc ctggcagcca tgatgtcagc cgttggtggt
     5641 gcgatgttta ccttgcaagt gggctttatg tctccttcct tcgttgggat tgtgccctca
     5701 attgaaatgg tgattttctg tgcggtgggc ggacgcgcat cattgattgg tgcagtctac
     5761 ggcaccttgc tggtcaatta cggtaaaacc gtgttctcgg aaacctatcc agagctgtgg
     5821 ctgttcctga tgggcggact gtttatctct gtggtgatgt tcttccctaa cggcctggcc
     5881 ggtatctggg acaaactgtc aaccaaatat ggctggttca aagacaaagc cgaggttgca
     5941 aaaaagcctg aggtaagtac ttccggggta gaagcacaag gcgtacttac cgaaaccaca
     6001 gaaaccatgt tggctgctga ctctaccgat gcggccacca aagcgccacg caagagagcc
     6061 agtaagaaag acactgggct tgaaagtagc aaaatgagag gagcagaagc atgagtaata
     6121 ccgacttcgt cttggctgta gaagatttga ccgtctcctt cgatggcttt aaagcggtgg
     6181 atagcctcac catgtatatc gacaaaaacg aattgcgtgt gctggtgggc cccaacgggg
     6241 ctggcaagac cacggtactg gacctgattt gcggtaaaac caaatccacg gccgggtcaa
     6301 tcaagtttaa taacactgaa ttgaccaaac tgtccgagca cgaaattgtg cgttcaggtg
     6361 tcggccgtaa attccagaac ccgtcgattt acgaaaacct gaccgtctac cagaacctgg
     6421 aagtgtctta tccgaaaggc cgcggcgtgt ttggcagcct gaccttcaag cgcacccagg
     6481 aggtagaaga tcgggtgcgc aaagtggctg cagacattat gttgaccgaa ttcctcgata
     6541 tggaatcagc tttgttgagt cacggccaga aacaatggtt agaaatcggc atgttgctga
     6601 tgcaagagcc tgagctgctc atgctggacg aaccagtcgc ggggatgagt gcgaaagagc
     6661 gtgaccagac agctgacctg cttaaccgta tctgccaaaa ccgctctgtg attgtgattg
     6721 aacacgacat ggagttcgtt aagaaaattg cacacaaagt cacggtgctg caccaaggca
     6781 aaatcctggc ggaagggccg atggagcaag tgcaggctga cgaaaaagtg attgaagtgt
     6841 atctgggcca ctaactgaag cgccattaat cttaggagca tcgcatgttt gaaattgaaa
     6901 atttgcaggt aagttatggc caaagtcagg tgattcatgg cctgaatttc actgctaaca
     6961 aaaatgaaac gctggccatc atgggccgta atggcatggg caaaaccacg ctgtttaaat
     7021 ccctgatggg tattttgcct agcaaggcga ccaaagccac tgtggatggc gtcgatctgc
     7081 aaggttcggc cacgcatgac cgtgtgatga agggcctggc ctatgtgccg caaggccgct
     7141 ggattttccc gaatatgacg gtgcaggaaa atattgaaac cggtttggaa aagtctgcga
     7201 cgcgtgaaat cccggatgat atttatgcct tgttcccagt cctgtttgat atgcgtaagc
     7261 gtaagggcgg taacttgtct ggtggtcaac aacagcaatt ggccattgcg cgtgcgctgg
     7321 tgactaatcc taaagtgttg ttgcttgatg agccaaccga agggattcag ccatccatca
     7381 ttaaagatat cgccaaggta ttgaatgaaa tccgcaaaat gcgcgaaatc accatcatcg
     7441 tctccgaaca ggtgttgagc ttcaccatgg aagtggcaga ccgcattatc gtgatcgata
     7501 aaggccgctt tattcatgaa gatacccgcg ataacgtcga tgcggccaag gtgaaaagtt
     7561 atctgtctgt gtaatggtct gctgcccatc agggcagcgc ttgtggcatg atgccggtat
     7621 ggatgattta aaccctgcga tttcatcgcc acatcaacgc attgcagtca aacactggca
     7681 agccaacctt gagcttggtt ttgccagacg cgacactcgc tcttacctgg cgcaccggtt
     7741 acacaagggg ccgctagtcg tacaaaaaag cctgcatccc gaaggtgacg atgtttgcca
     7801 tgcggtcgta gtgcatcccc caggtggtgt cgctggtggg gatgccctgc atttgcaagt
     7861 aaaagtgggc gagggcgcaa aagcgttatt gacgacgccg ggtgcgggca aatggtacaa
     7921 ggccaatggc gccatcgcaa cgcaaaaact cacgtttcaa gtcgaagcgg ggagtggtct
     7981 ggaatggttg ccacaggaaa atatcttgtt cgatgcggct gaagtcggat tcagcgccga
     8041 aattgacttg gccgctgatg ccacattggc tgcctgggac atcgtatgcc tgggacgtca
     8101 ggcacaacag gaggtctggc agcaaggtct gtacagattg aatcagcgta tcttacggaa
     8161 tagtcgcttg atctggaatg agcgtgcata tatgcaggca gatgacctga tcaaaaagag
     8221 ccgtgtaggt ctgggtggtg cggcagtgtt tggcacattt ttggtgtgtg ctggggcagt
     8281 ccctgcagag cttttgcaag cttgccgtca gattgctgtt tcagacgcta acacagggat
     8341 cacagcattg ccggatgtat tatgtattcg ctatgtcggt aatgcttctc agcaagcacg
     8401 gcagtatttt gaacagtgct ggcagatttt gcgaccatgg tattatcaaa aagacgcggt
     8461 cagaccacgt atctggaata cctgatttga gcaaacaata agatctactg agaatgtatc
     8521 tatggaactc accccaagag aaaaagataa gctattgata tttaccgcag cgttattggc
     8581 ggaacgacgc aaggcgcgtg gtttaaaact gaattacccg gaagccgtgg catttatttc
     8641 tgccgcgatt atggaagggg cacgcgatgg taaaacagtg gccgaactca tgagttacgg
     8701 gaccaccttg ctagcacgcg atgaggtcat ggaaggcgta ccggaaatga ttccggatat
     8761 acagattgag gcgacgtttc cggatgggac gaaattagtc accgtccacc acccgattcc
     8821 ataggtgtga ataatgaaaa tggcgaattg cggcgttgcg cggtactcga aatcctcatg
     8881 tactacatgt acattccggt ttctgcgttc cgggcgcttt gcacttcatc cattttgatc
     8941 attcactgac gatctaaaag attcaatagg aaagcatatg attcccggcg aaatgcaggt
     9001 cgcagcaggc gacatcgaac tcaatacagg tcgtgagacc gtcactttgg atgtcgcaaa
     9061 caaaggcgac agacctatcc aagtcgggtc gcattaccat ttttacgaaa ccaatgatgc
     9121 cttgtcgttt gaccgggcat tagcgtatgg ctgtcgcttg aatattgcag ctggcaccgc
     9181 ggtgcggttt gagccaggac agacgcgtac tgtacagctg gtcaagctgg caggggcacg
     9241 taaagtgtat ggctttgccg gacgtgtgat ggggggcttg aaatgaccct caagatggat
     9301 aaacatgctt acgctgaaat gttcggcccg actgtcggtg ataaggttcg tttggcagac
     9361 acggatttat gggtagaggt cgaaaaagac ttcactatct acggtgaaga ggtcaagttt
     9421 ggcggtggca aggtcatccg tgatggcatg gggcaagggc agggcctagc agcagaagtc
     9481 gctgataccg tgattaccaa tgccttaatt atcgatcact ggggtattgt gaaagccgat
     9541 atcggcatca agggtggcta tatttcagcc atcggcaagg ccggtaaccc ggatattcag
     9601 ccaggcgtga cgattgcaat tggtgcgggt acagaagtga ttgcgggtga aggcatgatt
     9661 atcaccgcgg gtggtattga tacgcatatc cattttattt gcccgcaaca gattgaagag
     9721 gctttgatgt ctggcgtcac aaccatggtc ggtggtggta ccggcccggc gacaggcaca
     9781 tttgcgacga cttgtacgcc aggaccgtgg catatccacc gcatgctgca atccgcggat
     9841 gctttcccga tgaatctggg ctttttggga aaaggtaatg ccagcctccc agagcctttg
     9901 cgtgagcagg tcaaagctgg cgtgataggc cttaagctgc acgaagactg gggaacgacg
     9961 cccgcagcga ttgataattg cctaagtgtg gccgatgaga tggatatcca ggtcgccatt
    10021 cactccgata cgttgaacga atccggcttt gttgagacga cgataggcgc ttttaaaggt
    10081 cgcaccatcc ataccttcca taccgaaggc gcggggggcg gtcacgcacc agatattatc
    10141 aaggcggcca gccacccaaa cgtgttgccg tcttcgacta acccgacgcg gccatttacc
    10201 gtcaatacca ttgatgagca tctggacatg ctgatggtgt gccatcatct ggatccggcc
    10261 attgctgaag atattgcttt tgccgaaagc cgtatccgcc gtgagacgat tgcggcagaa
    10321 gacattttcc atgacctggg cgccttctcc atgatgtcgt ccgattcgca agcgatgggc
    10381 cgtgtcggtg aagtcattat ccgtacctgg caaactgcgc ataagatgaa agtgcaacgt
    10441 ggccccttgg cggaagacgc tgctggcaat gataatttcc gtatcaagcg ctatattgcc
    10501 aaatacacca ttaacccggc gttgacgcat ggcatttcac atgtggtggg ttcggtagaa
    10561 gtgggtaaac tggccgactt ggtcgtgtgg aaacccgcat tctttggcgt gaaaccattc
    10621 accattatca aaggcggcat gattgctgcg gccgcgatgg gcgatccgaa tgcgtctatc
    10681 ccgacgccac agcctgtaca ttaccgcccc atgtttggtg cgtatggtgg tggtttgaaa
    10741 acagcggtga cctttgtgtc acaggccgca ttggaaagtc ctgaaatcaa ggcattaggt
    10801 ctgcaaaaac cactggtggc ggtcaaaggt acacgcgatg tgaaaaagtc agatatggtg
    10861 cataacacct ggatgcccaa gattgatgtt gatccggaaa cctatgaagt gctggccgat
    10921 ggtatgcctc tgatctgtga gccggcagaa gtattgccaa tggctcaacg ttactttttg
    10981 ttttaatcat gatccattta accgaacgaa tcactgtctg cggccatatc gacgatcaat
    11041 tagagttgcc ttttgatcag cgccagaaaa gccgtttgcg cgtcacgctg gcttctggca
    11101 aagaagctgc attattttta agccgcggca ttattttgcg tggcggcgat ttaattcagt
    11161 ctgaagacgg cagtgtggtg gtgcagattg tggccgcgca ggaacctgtc tataacgtga
    11221 ttgcgcctac cccacctgac ttgatgcgtg cagcgtacca tttgggcaac cgtcatgtac
    11281 ctttgcagat tggtgatggc tggttgcgcc tggagcaaga ttatgtgctg cgtgacatgt
    11341 tgctgggctt gggcatgcag gtcagcgaag tgcaagcccc gtttgaaccg gaagccggcg
    11401 cttatggcgg cggtcaccgt catgggcatg atgacgatgc gttgcagagc ttgcgcccaa
    11461 ggctgaaagg gcaggggcat taatggcgaa tgcactcgca ctcagccgct tgctgcaatt
    11521 ggccagccca ttattgcccg tgggcgctta tggctattcc caagggctgg agtgggcgat
    11581 tgagtctggt gaagtcaagg atggggcctc tgcacaagct tggataggcg atagtttgca
    11641 ggtatatcag gcacgttttg agttgccggt gttgtataag ttatatcagg cctggcaagc
    11701 aggtgatttg gcacaggtta aagagtggga tagcttctat caggctgggc gtgacacctc
    11761 tg
//
LOCUS       DATMSK010000091         4615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_100856,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000091 DATMSK010000000
VERSION     DATMSK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100856"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VNZ84_03155"
     CDS             complement(<1..827)
                     /locus_tag="VNZ84_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HWU65981.1"
                     /translation="MIQYNPKDWLTFIFRFHESDTFRTLTPMMILIGGYAGGIAYLEI
                     EYWKLADSSHVKNLSLIHTTVGFVLSLLLAYRTNTAYDRWWEGRKAWGALVNNSRNLA
                     MKLSAYLADEADRDFFRKIIPTYAAVLSKHLMNEDVSKMLFDGLDLEIDHHEHHPNQI
                     AQRLFQKVNELYTAGKISGDQFYIINDELKSFTDICGMCERIKNTPIPYSYSAFIKKF
                     IFFYVMTLPFGFVFSLGYYVIPVVVFIFYVLASLELIAEEIEDPFGTDTNDLPTPKMA
                     "
     gene            complement(978..1157)
                     /locus_tag="VNZ84_03160"
     CDS             complement(978..1157)
                     /locus_tag="VNZ84_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65982.1"
                     /translation="MTKALKEAKLEAHLDAIEKHKALLEKLHLNTNQHLDEVNNSLQE
                     LTLTLEEYLKIIGIP"
     gene            1305..1811
                     /locus_tag="VNZ84_03165"
     CDS             1305..1811
                     /locus_tag="VNZ84_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HWU65983.1"
                     /translation="MFSLLIQQFLPGYPAHLHAQVAQMHVQGELGTYIQKRYPQTHTI
                     QSDKALYDFCTELKQRYLKNAPLLDKVHFDNKMTVEKQTLGLNTAISRVQGGKLKAKK
                     EIRISAFFKTAPLEFLQMIVVHELAHLKERDHDKAFYQLCLHMEPNYMQYEFDCRVYL
                     LWLLLEKR"
     gene            complement(1808..2956)
                     /locus_tag="VNZ84_03170"
     CDS             complement(1808..2956)
                     /locus_tag="VNZ84_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWU65984.1"
                     /translation="MHRRSFLKWGGAGLATMLLPQLAFANAATDQRFVFIIQRGAADG
                     LNTLVPYADPDYAKLRGALAIQDTQTNRLDSLFALHPALSNAFALYQQQQAMFVHAIA
                     SPYRERSHFDGQNVLESGGTRAYQLKDGWLNRLLGLLPKAQSEAIAFSPTIPLALRGT
                     IQVDAYATAKLPQPPDDLLMRVSQLYAADAQLHTVWESALQTANMADMAGEGKNSQQP
                     EALGKLVGDFLSQAQGPRIAMLETGGWDTHSAQQARLAKQLADLDTLIASLKQSLGSH
                     WQQTLVIVATEFGRTAAANGTGGTDHGTGAAMLAFGGGLPAITARNKQWAAGKVIADW
                     PGLSNSQLYQGRDLQPTMALDKVIAELVSAHYGLDNPLVSRTLFPATA"
     gene            complement(2959..4341)
                     /locus_tag="VNZ84_03175"
     CDS             complement(2959..4341)
                     /locus_tag="VNZ84_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HWU65985.1"
                     /translation="MTNNDRLVYQHFGLGATPNSPAVDTDPVKALLDQLNHYQVMPEA
                     WKNYQSQQPSVEQAMRDTRQVNQADSADEKMAMRRQLRIAARDSYADAVQTRAWVAQQ
                     SETPFVERLVHFWSNHFAISVQKPLVTQFAGSFEVQAIRPHVLGRFKDMLMAVEKHPA
                     MLVYLDQQQSRGPDSMTMQRKMKRHPDKQAGLNENLAREILELHTLGVRSGYSQQDVT
                     EFARALTGWTLPNPQQTGDSEARYPGFMFNARVHEPGSRTLLGKTYAQNGVQQAEAIL
                     ADLAASPATASHLASKLARHFAGDQPPETLVNKLRDRYLQTGGDLTALYRVLVEAPEC
                     QANTAVKFKTPWEWLISSVRALDIHLEHIKVAQVMNQLGQSIWKPGSPAGYDDVADSW
                     LASNALMRRVEYAQRLATLTKLTQPPAEIAQRVLSGVLSPDTLKQIQRAESAQTGFAL
                     LLVSPEFLRR"
BASE COUNT         1002 a         1145 c         1277 g         1191 t
ORIGIN      
        1 gccattttcg gggtaggcaa gtcgttcgta tccgtgccaa acggatcttc aatttcttcg
       61 gcaatcagtt ccaggctggc gagcacataa aagataaata ccacgaccgg aatcacgtaa
      121 tagccgaggc tgaacacaaa gccaaacggc agcgtcatca cataaaagaa gatgaatttt
      181 ttgataaatg cgctgtatga gtaagggata ggcgtatttt taatccgttc acacataccg
      241 caaatgtcgg tgaaagactt gagctcatcg ttgatgatgt aaaactggtc accactgatt
      301 ttgcctgccg tgtaaagctc gttcactttt tggaacaggc gctgtgcaat ctggttggga
      361 tggtgttcgt ggtggtcaat ctccagatcc aggccatcaa acagcatttt actgacgtct
      421 tcattcatca ggtgcttgga aagcaccgcc gcataggtcg ggatgatctt cctgaaaaaa
      481 tcacggtcag cttcgtcggc caggtaggcg gacaatttca tcgccaggtt gcggctgttg
      541 ttgactaacg ctccccaggc tttgcggccc tcccaccagc ggtcataagc ggtgttggtg
      601 cggtaagcca acaacaatga caggacaaag ccaacggtgg tgtggattaa agagagattt
      661 ttgacgtgac tgctgtctgc cagtttccag tactcgattt caagataggc gataccaccg
      721 gcatagccgc cgatcaaaat catcatgggt gtgagtgtgc ggaaagtatc tgactcgtga
      781 aagcggaaga taaaggtgag ccagtcttta gggttgtatt gaatcatggt gggtatattt
      841 gggtagttgc gactgaatag agatgcgtca ccgagtgtag catgaaggcg cggcacatca
      901 tgtggccgtc gccatcacaa acagcacttt gctgagttga atgagttcag cccccattca
      961 acccgccgat gctttgatta cgggatcccg ataatcttca ggtattcttc aagggtcaaa
     1021 gtcagctcct gcaaagagtt attcacctca tcaagatgct ggttggtgtt taagtgcagc
     1081 ttttccagca aagccttgtg tttttcaatc gcgtctaaat gggcttcaag ttttgcttct
     1141 ttcagtgctt tggtcatgag gattctccaa tatgggctta cgttgaatat aagaggctta
     1201 ccgacgtcat taagtttatt actcctggct gggttgtcgt tgcgttgtat caatatgcgg
     1261 cgagtgtttg taagcggcca tgatttctgt tttacaatgc ggacatgttc agcctgctga
     1321 ttcaacaatt cttgcctggt tacccagcac acctgcacgc acaggttgcg caaatgcatg
     1381 tgcaaggcga gctgggcacc tatatccaga aacgttatcc gcaaacgcat actatccaaa
     1441 gcgataaggc cttgtatgac ttttgtactg agctcaagca gcgttatttg aaaaatgctc
     1501 ctttgctgga caaggtgcat ttcgacaaca agatgacggt agagaaacag accttgggcc
     1561 tcaatacggc gatttcacgt gtgcaaggcg gcaagctcaa ggccaagaaa gaaatccgga
     1621 tctcggcatt ttttaaaaca gcaccgctgg aatttttgca gatgattgtg gtgcatgagt
     1681 tggcgcatct caaagaacgc gaccatgaca aggcgtttta ccagctgtgt ttgcatatgg
     1741 aacccaacta catgcaatat gagtttgatt gccgagtcta tttgttatgg ctgctgcttg
     1801 aaaagcgtta agccgtggcc gggaatagtg tacggcttac caatggatta tctaagccat
     1861 agtgtgcact cactaattca gcaatgacct tatcaagtgc cattgtgggt tgcaagtcgc
     1921 ggccttgata tagttggctg ttactgaggc ctggccagtc ggcgatgact tttccggcgg
     1981 cccactgttt attgcgtgcc gtgatggcag gcaaccctcc cccaaaggcc agcatggcgg
     2041 cacctgtgcc atgatcggta ccgccggttc cattggctgc ggcagtgcga ccaaactcgg
     2101 tagcgacaat caccagggtt tgctgccaat gactgcccag cgattgtttc aggctggcga
     2161 tcagtgtgtc caggtctgcc agctgtttgg ccagtcgcgc ttgttgggcg ctgtgggtat
     2221 cccagccgcc tgtctctagc atggcaatgc gtgggccttg agcctggctt aaaaagtcgc
     2281 ctaccagttt gcccaatgct tccggttgct ggctgttttt cccctcaccg gccatgtcgg
     2341 ccatattggc ggtttgcagc gcgctttccc agacggtatg taattgcgca tcggccgcat
     2401 acaactggct cacccgcatc agcaagtcat ccggtggttg cggtagtttt gcggtggcat
     2461 aggcatccac ttggatagta ccgcgcagtg ccagcggaat cgtcggcgaa aaagcgatgg
     2521 cttcactttg cgctttgggc agtaaaccca gtaaacgatt gagccagcca tctttaagct
     2581 ggtaggcgcg tgtgccacca ctttcaagta cattttgtcc atcaaaatgc gagcgttcgc
     2641 ggtagggtga ggcgatggca tgtacaaaca tggcttgctg ctgttgatag agggcaaagg
     2701 cattgctgag ggcagggtgt agtgcaaaca ggctgtccag ccggttggtt tgcgtatcct
     2761 ggatcgccag tgcaccccgc aatttggcgt agtcaggatc cgcgtacggt accagggtat
     2821 tcagtccatc agctgcgcca cgctggataa taaacacgaa gcgctggtcg gtggcggcat
     2881 tggcaaaagc cagctgtggc agcaacatgg tggccagtcc ggcaccgccc cattttaaaa
     2941 agcttctgcg gtgcatggct atctcctcaa aaattccggg ctgaccagca gtagtgcaaa
     3001 gccggtctgg gcggattcgg cacgctgtat ctgttttaag gtatctggac tgagtacgcc
     3061 actgagtaca cgctgggcaa tctctgccgg tggttgtgtc agtttagtca gcgtcgccaa
     3121 ccgctgggcg tactctaccc gtcgcatcag ggcattcgag gccagccagc tgtcggcaac
     3181 atcatcataa ccggcaggcg aaccaggctt ccagatagac tgccccaact ggttcatgac
     3241 ttgtgccact ttgatatgct ccaggtggat gtcgagcgcc cgcacgctgc tgatcaacca
     3301 ctcccaaggt gttttgaatt tcacggccgt gttggcctgg cattctggcg cttctaccag
     3361 cacgcggtat aaggctgtca ggtccccgcc agtttgcaga tagcggtcac gcagtttatt
     3421 caccagggtt tctggtggct ggtcgccggc aaagtgcctg gccagtttgc tggccaggtg
     3481 tgaggcggtc gcgggggatg ccgccaggtc ggccaggatg gcttcagcct gctgtacgcc
     3541 gttttgtgcg taagttttac ccagtagtgt ccggctgccg ggttcatgca cccgggcatt
     3601 gaacataaat cccgggtaac gtgcctcgct atcgcctgtt tgttgcgggt tgggtagtgt
     3661 ccagccggtc agtgcacgcg caaattcggt gacatcctgc tggctgtaac cactgcgtac
     3721 tcccagcgta tgcagctcca gaatttcgcg tgccagattc tcgttcaggc cggcctgttt
     3781 gtcgggatga cgcttcattt tccgttgcat cgtcatactg tctggcccgc gtgattgttg
     3841 ctggtcgaga tagaccagca tggcaggatg cttctcaacc gccatcagca tgtctttaaa
     3901 gcggcccagt acatgcgggc ggatggcctg gacctcaaag ctgcccgcaa attgcgtgac
     3961 cagcggcttt tgtacggaaa tggcaaagtg gttagaccaa aagtgcacca ggcgttcgac
     4021 aaagggggtt tctgactgct gcgccaccca ggcccgtgtc tgtaccgcat cggcataact
     4081 gtctctggca gctatccgta actggcggcg catggccatt ttttcatccg ctgaatcggc
     4141 ttggttgacc tgtcgtgtgt cgcgcatggc ctgttcaacg gagggttgct ggctttggta
     4201 gtttttccag gcttcaggca tgacctggta gtggttgagt tgatcaagca aggccttgac
     4261 cgggtcggtg tcgaccgcag gcgaatttgg ggtggcacct agtccaaaat gttgatacac
     4321 aagacggtca ttgttggtca tacagttgtc cagtcaggtg atgcggcagc aggtgattgc
     4381 ataataccgt gatgtttatg ctgcatgtgt agcaaatatg gcagttgtca gcttgctgtc
     4441 agcaagcgtt tagtccatta catcggtcga gtcacttgta gccagaccgc ggctatcctg
     4501 tccgcttcaa tatacgctgt gttcaacagc tcttcgctga aaatgtcagg gtcaatttcg
     4561 gaatattggt agcttgcccc tgccttgttg attttttctt cgaccgcatc aagaa
//
LOCUS       DATMSK010000092         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_241940,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000092 DATMSK010000000
VERSION     DATMSK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241940"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /gene="rfbB"
                     /locus_tag="VNZ84_03180"
     CDS             <1..964
                     /gene="rfbB"
                     /locus_tag="VNZ84_03180"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWU65986.1"
                     /translation="NMENLADINQHPNHIFVHADIGDRHAVQELLKTHRPRAVLNLAA
                     ESHVDRSIHGPDAFIQTNVVGTFNLLEEVRAYWQALPTDEQAQFRFLHVSTDEVYGSL
                     GDHDPAFKETNPYQPNSPYSASKAASDHLVRAYHHTYGLPTLTTNCSNNYGPYHFPEK
                     LIPLVIHNALSGKPLPIYGNGSNIRDWLYVEDHCSAIRQVLVTGIPGETYNIGGWNEK
                     TNLDVVHTLCDILDQERPKAQGSYRDQITFVKDRPGHDLRYAIDASKIERELGWKPQE
                     TFETGIQKTVRWYLGNPEWVEHVTSGEYQHWLNKNYHERGEELA"
     gene            964..1845
                     /gene="rfbA"
                     /locus_tag="VNZ84_03185"
     CDS             964..1845
                     /gene="rfbA"
                     /locus_tag="VNZ84_03185"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HWU65987.1"
                     /translation="MARKGIVLAGGSGTRLYPVTQVVSKQLLPVYDKPMIYYPLSALM
                     LAGIREILIISTPMDTPRFKELLGDGSQWGMRIEYQVQPSPDGLAQAFILGESFIGND
                     TSALVLGDNIFYGHELGAMAMAAGHREQGATVFAYPVNDPERYGVVEFDANGAAISLE
                     EKPVQPKSRYAVTGLYFYDQRVVDIAKNLKPSPRGELEITDVNKQYLAWGNLNVEVMG
                     RGMAWLDTGTHESLLEASGFIQTIEKRQGLKIACLEEIAYRQGFINAEQLDKLADKYA
                     KNGYGQYLKQILKEQVF"
     gene            1845..2390
                     /gene="rfbC"
                     /locus_tag="VNZ84_03190"
     CDS             1845..2390
                     /gene="rfbC"
                     /locus_tag="VNZ84_03190"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HWU65988.1"
                     /translation="MQVIATAIPEVLILEPKVFGDARGFFLESFNAQTFKVSTGLDVQ
                     FVQDNHSRSAKGVLRGMHYQMQQTQGKLVRVVQGEVFDVAVDMRQHSPTFGQWAGVHL
                     SAENHRQLWMPAGFAHGFVVLSEFADFLYKTTDYYAPQHEVCLKWDDPTVGIDWPLPC
                     EPNLSSKDQQGFSWVNAPKFD"
     gene            2407..3324
                     /gene="rfbD"
                     /locus_tag="VNZ84_03195"
     CDS             2407..3324
                     /gene="rfbD"
                     /locus_tag="VNZ84_03195"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HWU65989.1"
                     /translation="MKKILLTGVNGQVGHALLQQMKQMDAFEVVACGRQQLDLTEPEQ
                     IRRVVRTVQPDLIINPAAYTAVDKAESERELAYAINAAAVKVLAEEAARLNTGLIHFS
                     TDYVYDGTKSSWYVETDAVNPVSVYGASKLAGEEAIRKAGVPHLILRTSWVYGSYGKN
                     FMKTIIRLAQEREVLRIVADQHGAPTSDISIAQAVLQLLSHWQPDDSAQSGVYHLVNA
                     GETTWYGFAKAIEAQYRALAGQASWPALALKEIKPITTEEYPTPAKRPANSRLSTEKL
                     AQQFGVTLPEWPQALNQVMAELDFRSANT"
     gene            complement(3330..3776)
                     /gene="queD"
                     /locus_tag="VNZ84_03200"
     CDS             complement(3330..3776)
                     /gene="queD"
                     /locus_tag="VNZ84_03200"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HWU65990.1"
                     /translation="MEITTRLEFDAGHRIPDHKSQCRNLHGHRYAIEITLSGDIIQAE
                     HASENGMVMDFADVKRIARETIVDRWDHAFLVYQGDAEVLNFLNSLPNHKTIIFPTIP
                     TAENMAAEAFKLLKAQYRDTFGNHLKLVKVRLYETPNSWTDAFDPV"
     gene            complement(3852..>4032)
                     /locus_tag="VNZ84_03205"
     CDS             complement(3852..>4032)
                     /locus_tag="VNZ84_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU65991.1"
                     /translation="YIMLQKTTPTDKFKKIDQMLEDFAGDEMDDFDTGYSSYEKSHKH
                     YSADEDALMNFDDLY"
BASE COUNT         1039 a          897 c         1034 g         1062 t
ORIGIN      
        1 taatatggaa aacctggcag atatcaacca gcacccaaac catatttttg tccatgccga
       61 tattggtgat cgtcacgctg tacaggagtt gttgaagacg cacagacctc gtgctgtact
      121 caacctggcg gccgaaagcc atgttgaccg ctctattcat ggccctgacg cgtttattca
      181 gaccaatgtg gtcggcactt ttaatttgct tgaagaggtt cgcgcctatt ggcaagcttt
      241 gcctaccgat gagcaagcgc agttccgctt cctgcatgtg tctaccgatg aagtctatgg
      301 gtcgctagga gatcatgatc cggcattcaa ggaaaccaat ccataccagc cgaatagccc
      361 ttactctgca tccaaggcag cttctgacca tcttgtacgt gcatatcacc atacctacgg
      421 tttgccgaca ctgacaacta actgttccaa taattacgga ccatatcact ttcctgaaaa
      481 attaattcca ctggtcatac ataatgcttt gtctggtaaa ccgctgccta tttacggcaa
      541 tggtagcaat atccgggatt ggttgtatgt agaggatcat tgcagtgcga ttcgccaagt
      601 gctagtaacg ggtattccag gcgagaccta caatatcggt ggctggaatg agaaaaccaa
      661 cctggacgtt gtgcatactt tgtgcgatat cctcgatcag gaacgtccaa aagcgcaggg
      721 tagttaccgc gaccagatta cttttgtcaa agaccgccct ggccatgatt tgcgttacgc
      781 gattgatgcc agcaaaattg agcgggaact cggctggaaa ccacaggaaa cctttgaaac
      841 cgggatacag aaaacagtcc gctggtatct tggaaatccc gaatgggtgg agcatgtcac
      901 cagcggtgaa tatcagcatt ggctaaacaa aaactaccac gaacgtggag aggagctagc
      961 ataatggcaa gaaaagggat tgtattggct ggtggttccg ggactcgctt gtatcctgtc
     1021 acgcaagtgg tttccaagca gctgttgccg gtgtatgaca agcccatgat ttattatccg
     1081 ctgtcggcac tgatgctggc tggcatacgc gaaatactga ttatttctac ccctatggat
     1141 acgccacgct ttaaggagtt attgggtgat ggtagccagt ggggcatgcg tatcgaatac
     1201 caggttcagc catccccaga tggcctggca caggccttta tactgggtga gtcatttatc
     1261 ggcaatgaca ccagcgcgct cgtgctggga gataatattt tttatggtca tgaactgggt
     1321 gccatggcca tggcggccgg gcatagagag cagggggcaa ccgtgtttgc ttatcctgtg
     1381 aatgatcctg agcgttatgg tgttgtagag tttgatgcca acggggcagc gattagcctg
     1441 gaagagaagc cggtgcaacc aaaatcgcgt tatgcagtca ccggattata tttttatgac
     1501 caacgtgtgg tcgatatcgc caaaaacctc aaaccatctc cacgtggtga gttagagatt
     1561 acggatgtca ataaacaata cttggcctgg ggaaatctca atgttgaggt gatgggtcgt
     1621 ggtatggcgt ggctggacac cggtacacat gagtctttac tcgaagccag tggctttatt
     1681 caaacgattg aaaaacgtca gggcctgaag attgcctgtc tcgaggaaat tgcctatcgc
     1741 caggggttca ttaatgccga acaattggac aaattagcag ataagtatgc caaaaatggt
     1801 tatgggcaat atttgaaaca aattcttaaa gagcaggtgt tttaatgcaa gtcattgcaa
     1861 cggcgattcc agaagtactc attctggagc ccaaagtatt tggggatgcg cgtggctttt
     1921 ttctggaaag ctttaatgcc cagactttca aagtaagtac agggctggat gtacagtttg
     1981 tccaggataa ccattctcgt tcagccaaag gggtgctgcg aggaatgcat taccaaatgc
     2041 agcaaaccca gggtaagctg gttcgtgtcg tgcaaggaga ggtctttgat gtggcagtgg
     2101 atatgcgtca gcattccccc actttcgggc aatgggctgg cgtgcatcta agtgcagaaa
     2161 accatcgcca gctttggatg ccagctggtt ttgcgcatgg gtttgtggtg ctgtcagagt
     2221 ttgcagattt tctatacaaa accaccgatt actatgcacc gcaacatgag gtctgtttga
     2281 aatgggatga tcccaccgtt ggcatagact ggccgttacc atgcgagcct aacttgtcgt
     2341 ccaaagacca gcagggattt tcctgggtta atgcgcctaa attcgattaa acctctccca
     2401 cttgatatga aaaaaattct gttaaccggc gtcaatggtc aggtcggtca tgcattgttg
     2461 cagcaaatga aacagatgga tgcgtttgaa gtcgttgcct gtggtcgcca gcagcttgat
     2521 ttaacagaac ctgaacagat caggcgtgtg gtgcgtactg ttcaacctga tttgattatt
     2581 aatccggccg cttataccgc tgtagataaa gcagaatctg agcgtgagct ggcttatgcg
     2641 atcaatgcag cggcagttaa ggtgttagcc gaagaggcgg ccagactcaa tacaggcttg
     2701 attcatttct cgaccgatta tgtttacgat ggtacaaaat cttcatggta tgttgaaaca
     2761 gatgcggtta atcccgtcag tgtttatggt gccagcaaat tggcgggcga ggaggccatt
     2821 cgtaaggcag gtgtgcccca tttgattttg cgtaccagct gggtgtatgg cagctatggc
     2881 aaaaatttca tgaagacgat tatccgcctg gcccaggagc gtgaggtact acgtattgtg
     2941 gctgatcaac acggggcacc tacatcggat atttccatag cacaagccgt gttgcaattg
     3001 ctcagtcact ggcagcctga tgactcggca caaagcggag tttatcatct ggtcaatgcc
     3061 ggggagacga catggtacgg gtttgctaaa gcgattgaag cccaatatag agccctggca
     3121 ggccaggcca gttggcctgc gctggcatta aaagagataa agccgattac aacggaagaa
     3181 tacccaacac cagccaaacg tcccgccaac tcaagacttt caacagaaaa actggcccag
     3241 cagtttgggg taacgttgcc agagtggccg caggctttga atcaagtgat ggccgaactg
     3301 gatttcaggt cagctaacac ttagctacgt taaactgggt cgaacgcatc tgtccagctg
     3361 ttgggagtct catacaggcg tactttgact agcttaaggt gattgccaaa agtatcgcgg
     3421 tattgtgctt tgagtaattt aaaagcttct gctgccatat tttcggcagt gggaatggtt
     3481 gggaagataa tggttttatg attgggcaga ctgttcagaa agttcaggac ttctgcatcg
     3541 ccttggtaaa ccagaaaagc gtggtcccag cggtctacaa tcgtttcgcg ggcaatacgc
     3601 ttcacatcgg caaaatccat caccattccg ttttctgaag catgttctgc ctgaatgatg
     3661 tcaccggata gcgtaatctc tatcgcataa cgatgaccgt gcaaattgcg gcattgactt
     3721 ttatgatcgg gaatacgatg tcccgcatcg aattccaagc gtgtagtaat ttccatcacg
     3781 ctattatatc agcaaccatg acgcgttact gagcctggct aaatagcttt ttcacctcga
     3841 tgagactgtt attaatacaa gtcgtcaaag ttcattagtg catcttcatc ggcagagtaa
     3901 tgcttgtggc ttttttcgta gctgctgtag ccagtgtcaa aatcgtccat ttcgtcacca
     3961 gcgaaatctt ccagcatctg gtcgattttt ttaaatttgt cggtaggtgt tgttttttgt
     4021 agcatgatgt ac
//
LOCUS       DATMSK010000093         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_289167,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000093 DATMSK010000000
VERSION     DATMSK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289167"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VNZ84_03210"
     CDS             <1..854
                     /locus_tag="VNZ84_03210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWU65992.1"
                     /translation="RLNEKNTTHLFNKHIANTDIDSAKVLVERFNQVVTDVTSSTQKA
                     GLDTAQFRKTLDAWSDEIQQMDAQLGAQLGSLLSDTVHMQTSMHTLQNKLESSLEEIA
                     DLKNEIMKAKQEAISDGLTGLMNRKGFDTALHQCLDTVANDETCLVIADIDFFKRVND
                     SYGHLFGDKVIQAVAKILKTVSGNLYTAARYGGEEFVLLLPHTSVDIAKEIAESVRRQ
                     VAAISIKNNVTQRLISNITISLGVTSYQPGESVDSFITRADQALYTSKAHGRNQVTMI
                     TEALAAA"
     gene            complement(880..1308)
                     /locus_tag="VNZ84_03215"
     CDS             complement(880..1308)
                     /locus_tag="VNZ84_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HWU65993.1"
                     /translation="MKPPYEHRTDKMAHQALMWIERLGLGLVTIATFFSLAHELARMW
                     NAGMVGLGDLLQLFLYLEVFSMINSYFGTGKLPMRYPIYIAMVALARYLLLDMKEMTE
                     THMLAVSAAILILAISVLVMRFGHSQYPYEDIPEQKRDLL"
     gene            complement(1305..1988)
                     /locus_tag="VNZ84_03220"
     CDS             complement(1305..1988)
                     /locus_tag="VNZ84_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWU65994.1"
                     /translation="MRAMILAAGRGERMRPLTDHTPKPLLAVGGKPLIVWHIERLARA
                     GFRELVINHAHLGAQIEAALGDGRQWQVSICYSAEATALETAGGIANALPLLGDLPFL
                     VINGDVFTDIDYADLTLPADRLAHLVMVDNPPQHAAGDFGLEHGFVLDSAEHRLTFSG
                     VGVYHPELFSSIQHGQAAKLAPLLRAAMQQQRVTGQHHHGLWHDIGTPERLAQLDQWI
                     QENNHQEEA"
     gene            complement(1991..>2170)
                     /locus_tag="VNZ84_03225"
     CDS             complement(1991..>2170)
                     /locus_tag="VNZ84_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HWU65995.1"
                     /translation="GVQQHIKVLGIFARLYHRDGKDGYLKDIPLVMQYLQAACERYVE
                     LRPFFKLLLTLEKAE"
BASE COUNT          594 a          588 c          498 g          490 t
ORIGIN      
        1 aaaggcttaa cgaaaaaaat acgacccacc tcttcaacaa gcatatcgcc aacacagata
       61 tagacagcgc aaaagtactg gttgagcgct ttaaccaggt tgtcaccgac gtgacttcat
      121 ctacccaaaa ggctgggtta gatactgcgc aattcaggaa aacactggat gcctggtccg
      181 atgaaatcca acagatggat gcccaacttg gcgcccaatt gggttcactc ttgtccgaca
      241 cggtgcatat gcagacctcc atgcataccc tgcaaaacaa gctggaatca agccttgaag
      301 aaattgcaga tctcaaaaat gaaattatga aagccaaaca ggaagcgatt tctgatggtt
      361 tgacaggcct gatgaaccgc aagggatttg atacggcctt gcaccaatgc ctggatacag
      421 tggccaatga cgaaacttgc ctggtaattg ccgacatcga ctttttcaag cgcgtcaatg
      481 acagttacgg ccatttattt ggtgataaag tgattcaggc cgtggccaag attttaaaaa
      541 ctgtttcagg caacctgtac actgcagccc gttatggcgg agaagagttt gtactgttat
      601 tgccgcatac cagtgtagac attgccaaag agattgcaga atcagtacgc cgtcaggtcg
      661 cggccatcag cattaaaaac aatgtgacac aacggctgat ttccaatatc acgatttcac
      721 tgggtgtcac gtcttaccag ccgggcgaat ctgtggatag tttcatcacg cgtgcggatc
      781 aggcgcttta cacctcaaaa gcacatggcc gcaatcaggt caccatgatt accgaagcgc
      841 tggctgccgc ctgacctacc accgcccaaa gggctgattt tatagcaagt cgcgcttttg
      901 ctcgggaata tcctcataag gatattgtga gtgaccaaat cgcatgacca atactgaaat
      961 tgccaatatg agaatggctg cagacaccgc cagcatatgg gtctcggtca tttccttcat
     1021 gtccagcaat aagtaacgcg ccaaagcgac catggcaata taaatcgggt agcgcatagg
     1081 caacttaccc gtaccaaaat agctgttgat catggaaaag acttccagat acaagaacag
     1141 ctgcaataaa tcgcccagcc cgaccatacc ggcattccac atacgtgcca attcgtgtgc
     1201 cagtgagaag aaggttgcga tggtaaccaa ccccaagccc agacgctcta tccacatcaa
     1261 tgcctggtgt gccattttat cggtacgatg ttcatatggt ggcttcatgc ttcctcctga
     1321 tgattatttt cttgtatcca ctgatcaagt tgcgccaaac gctccggcgt gcctatatca
     1381 tgccacaacc catggtgatg ttgccccgtc acgcgctgtt gttgcatggc tgcccttaaa
     1441 agaggcgcca gttttgcggc ctggccatgt tggatactac tgaataattc cggatgatag
     1501 acccctaccc ccgagaatgt cagccggtgc tcagcagaat ccaagacaaa gccatgttcc
     1561 aggccaaaat caccagcggc atgctgtgga ggattatcca ccatcaccag gtgtgccagc
     1621 ctatcggcag gcagcgtgag atccgcataa tcaatatccg tgaaaacatc gccattgatg
     1681 accagaaaag gcagatctcc caataatggc aaagcattgg cgatgccccc tgccgtctcc
     1741 aaagccgtcg cttccgcact gtaacaaatg ctgacttgcc attgtcgacc gtcaccaagc
     1801 gccgcttcaa tttgcgcccc caggtgggca tgattaatca ctaactcgcg gaacccggca
     1861 cgggccagtc gttcaatatg ccaaacgatc aacggtttac cgccaacagc caataatggt
     1921 tttggcgtgt gatcggtcaa aggccgcata cgctcgcccc gaccagccgc cagaatcatt
     1981 gctcgcatgg ctattctgcc ttttccagcg tcagcaataa tttaaaaaaa ggccgcaact
     2041 ctacatagcg ctcgcacgct gcctgcaggt actgcataac cagtgggata tccttgaggt
     2101 agccatcctt gccatcacga tgatacaagc gggcaaaaat gcccagcacc ttgatgtgct
     2161 gctgtacgcc
//
LOCUS       DATMSK010000094         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_7260, whole
            genome shotgun sequence.
ACCESSION   DATMSK010000094 DATMSK010000000
VERSION     DATMSK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7260"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VNZ84_03230"
     CDS             complement(<1..331)
                     /locus_tag="VNZ84_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWU65996.1"
                     /translation="MTEHTLLDSETPETALIPLLPLRDVVVYPHLVIPLFVGRSKSVK
                     ALERASEGDKKILLVAQKSANKDDPVAEDLYEIGTLATVLQMLKLPDGTVKVLVEGLH
                     RVRVSDFV"
     gene            complement(467..1756)
                     /gene="clpX"
                     /locus_tag="VNZ84_03235"
     CDS             complement(467..1756)
                     /gene="clpX"
                     /locus_tag="VNZ84_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWU65997.1"
                     /translation="MANKSDSEKLLYCSFCGKSQHEVKKLIAGPSVFICDECVDLCND
                     IIREEIQSLNEIKSADKKLPTPQEICSILDQYVIGQVQAKKNLAVAVYNHYKRLGYNP
                     LSDGSQSSKDDVEVQKSNILLIGPTGSGKTLLAQTLARLLDVPFVMADATTLTEAGYV
                     GEDVENIMQKLLQKCDYDVEKAQRGIVYIDEVDKISRKSDNPSITRDVSGEGVQQALL
                     KLIEGTIASVPPQGGRKHPNQEFVQLDTTNILFICGGAFDGLEKVIRHRSEKGGIGFG
                     AEVKSKEDVRAVGEVLREVEPEDLIKFGLIPEFIGRLPVVATLESLDEAALMTILVEP
                     KNALTKQYMKLFKMEGVELEFEESALRLIAKKALERKTGARGLRSIMEHSLLDLMYEL
                     PSLKSLSKVIVDAGVIEHSSEPILIYQDSQKRVENAS"
     gene            complement(1784..2431)
                     /gene="clpP"
                     /locus_tag="VNZ84_03240"
     CDS             complement(1784..2431)
                     /gene="clpP"
                     /locus_tag="VNZ84_03240"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HWU65998.1"
                     /translation="MNYMNANDSMMQQRMEPQGLGYIPMVIEQSGRGERSYDIYSRLL
                     KERVIFLVGPVNDMTANLVVAQLLFLEAENPDKDISLYINSPGGSVTAGMSIYDTMQF
                     IKPDVSTLCIGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGFQGQASDIEIHAK
                     EILYLKDKLNGIMAKHTGQDIETIANDTDRDNFMSADAAKAYGLVDQVIAARNAA"
     gene            complement(2435..>3445)
                     /gene="tig"
                     /locus_tag="VNZ84_03245"
     CDS             complement(2435..>3445)
                     /gene="tig"
                     /locus_tag="VNZ84_03245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWU65999.1"
                     /translation="AAEEFEYIATFEIFPEVVVGDLAKVKIERPTLELTDADVEKTLD
                     VLVKQRVSYEPVKRMSKKGDRINITLTASIDGQQVESTGDRGIDLVIGEPGRVKEFDD
                     ALVGGKAGAEKTFDITYPADHNPAQLAGKTVTYDVKFVSVSQPVYPEVDAEFAKNLGV
                     EDGNLDTMKAEIKQSLEQEVDKRIKARVKEAVFQALVDESSFELPKSIVSAEVNRLMQ
                     VAAQNLRQRGVDPAQVPMEPSAFEAQAQRNAKLRLVLTELVNSNNLQATADQVRAMVD
                     TFAQSFEKPDELVNWYYADVKRLDEPAALATEENVVAWVLDKAKVTNKKIKFDELMMA
                     GA"
BASE COUNT          874 a          908 c          721 g          942 t
ORIGIN      
        1 ccacaaagtc ggaaacgcgc acacgatgca agccctctac cagtactttg acagtgccgt
       61 caggcaactt cagcatttgc aatacggtcg ccagagtgcc aatttcataa aggtcttccg
      121 caaccggatc gtctttattg gcagactttt gggcaaccaa taaaatcttt ttatcccctt
      181 ctgaggcgcg ctccaaggct ttgaccgact tactacggcc aacgaacagc ggaatgacca
      241 aatgcgggta gacgacaaca tcacgcaatg gcagcaatgg gattaaagca gtttcaggcg
      301 tctcggagtc aagcaaagtg tgttcagtca tagcttcttt cgtaaagtgt caagggaact
      361 agccccttgc atttagatgg gaacattctt tgagaattca agagaacaag catctaatgt
      421 tgtagatgct atcttgcaat agccggacgg ctattgcaag cgggatttag ctggcgtttt
      481 caacgcgctt ttgtgagtcc tgataaatca ggatgggctc ggaagagtgt tcgatgaccc
      541 ctgcatctac aatcaccttg gacaggctct tgagtgatgg caattcgtac atcagatcca
      601 gcaatgaatg ctccatgatg gaacgcaggc cacgggcacc ggttttacgc tccagtgctt
      661 tcttggcaat cagacgcaag gcggactctt caaactccag ctccacacct tccatcttga
      721 acaacttcat atactgcttg gtcagtgcat tttttggttc gaccaaaata gtcatcaaag
      781 cggcctcatc caaggactcc agcgttgcaa ccacaggcaa acggccgatg aactcaggaa
      841 tcaggccaaa cttgattaag tcttcaggct caacctcacg cagcacttca ccgacagcgc
      901 gcacgtcttc cttgctcttc acttctgcac caaaaccaat accacctttt tcggagcgat
      961 gacgaatgac tttttccaga ccatcgaatg caccaccgca gataaacaga atattggtag
     1021 tatccagctg cacaaactcc tggtttggat gcttgcgtcc gccttgtggg ggaacagaag
     1081 caatggtgcc ttcaatcagc ttcaataaag cctgctgcac accctcacca gacacatcac
     1141 gtgtaatgga tgggttatca gacttgcgcg aaatcttgtc cacttcatca atgtacacaa
     1201 taccgcgctg ggctttctca acgtcgtaat cacacttttg cagtaatttc tgcataatat
     1261 tttcaacgtc ttcacctaca tagcctgctt cagtcaatgt tgtcgcatca gccatgacaa
     1321 atggcacatc gagcaagcgt gccagtgttt gtgccagtaa tgtttttcca gaacctgttg
     1381 ggccgatcag caagatattg ctcttctgca cttcaacatc atctttactg gattggctgc
     1441 catcagaaag tgggttatat cccaaccgct tgtagtgatt gtacaccgct acagccaggt
     1501 tctttttcgc ttgcacctga ccaatcacgt attggtcaag gatggagcat atttcctgtg
     1561 gtgtcggcag ttttttatcc gcagacttga tctcgttcag gctctgaatt tcttctcgga
     1621 taatgtcgtt gcacaaatca acgcattcat cacaaatgaa tacagatgga cccgcaatca
     1681 gttttttcac ctcatgctgg ctcttaccgc aaaacgagca gtataaaagc ttctcgctat
     1741 cagatttgtt tgccatactc ttcccctgct ggttgctcgc tgcttaagca gcgttacgtg
     1801 cggcaatcac ctgatctacc aagccgtaag ctttggcagc atcagcgctc ataaaattgt
     1861 cacgatcagt atcgttagca atcgtttcaa tatcttggcc tgtatgttta gccatgatgc
     1921 cattcaattt gtccttgaga tacaggattt ccttggcatg aatctcgatg tcagatgcct
     1981 gcccctggaa accacccaat ggctgatgaa tcatgacacg agagttaggc agacagaaac
     2041 gctttccttt ggcgccagca gttagcagga acgcgcccat gctggctgcc tgaccaatac
     2101 acaaagtgct tacatccggc ttgataaact gcatggtgtc ataaatagac atccctgcag
     2161 tcacagagcc accaggagaa ttgatgtaca aggaaatatc cttgtctgga ttctcagctt
     2221 ccaggaacaa caactgcgca acaaccaggt tagccgtcat gtcattgacc ggcccaacca
     2281 ggaaaatcac gcgctctttc aataagcgtg aatagatgtc atatgaacgc tcacctcggc
     2341 cactttgctc aatgaccatt ggaatgtagc caagtccttg tggctccata cgttgttgca
     2401 tcatgctatc gttcgcgttc atataattca tggtttaagc acctgccatc atcaattcat
     2461 caaatttaat ttttttgtta gtcactttag ccttatccaa gacccaagcc accacatttt
     2521 cttcagttgc cagtgcagca ggctcgtcca gacgtttaac gtctgcataa taccaattta
     2581 ccagctcgtc aggcttttca aagctttgtg caaaggtatc taccatggcg cgcacttgat
     2641 cagcggttgc ttgcaagtta ttgctgttca ccagttctgt caataccaga cgcaacttag
     2701 cgttgcgttg cgcttgcgct tcaaatgcag aaggctccat tggcacttgc gctggatcca
     2761 cgccacgttg acgcaaattt tgcgcagcga cttgcattaa acgattgact tcagcggaca
     2821 caatcgattt aggcagttca aaactgcttt cgtctactaa tgcctggaat acagcctcct
     2881 tgacacgtgc cttgatgcgt ttgtcaactt cctgctccag actctgtttg atttcagcct
     2941 tcatggtatc caggtttcca tcttccacac cgaggttttt ggcaaattcg gcatctactt
     3001 ctgggtaaac cggctgtgaa acggacacga acttcacgtc gtaagtgact gttttaccag
     3061 ccaattgcgc tggattatga tctgctgggt aagtaatatc gaacgttttt tcagctccag
     3121 ccttgccgcc taccaaagca tcatcaaatt ctttgacgcg gcctggttca ccaatcacca
     3181 aatcaatccc acgatcgcca gtgctttcga cttgctgacc atcaatagaa gccgttaatg
     3241 taatattgat gcggtcacct tttttggaca tgcgcttgac tggctcgtag ctgacacgct
     3301 gcttcactaa cacatccaat gttttttcaa catcggcgtc agttaactcc agtgttggac
     3361 gttcaatttt cactttggcc aggtcaccca ccaccacttc tgggaaaatc tcaaaagtag
     3421 caatgtattc aaactcttca gccgc
//
LOCUS       DATMSK010000095        16372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_77437,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000095 DATMSK010000000
VERSION     DATMSK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 16372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16372
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77437"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /gene="cobO"
                     /locus_tag="VNZ84_03250"
     CDS             <1..561
                     /gene="cobO"
                     /locus_tag="VNZ84_03250"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWU66000.1"
                     /translation="RKKAVIDEKIEQANQEKGLLLILTGNGKGKSSSAFGMVARALGH
                     GMRVGVAQFIKGRSDTGEEAFFRQHPAVTWHVLGEGFTWNTQDRQRDTETAMRGWQIV
                     AQMLQDPAINLVVLDELTYTFKYGYLDEQMVLDAIANRPPMQHVVITGRGASEALRNA
                     ADTVSELMDVKHAWRAGIKAQAGIDL"
     gene            561..1847
                     /locus_tag="VNZ84_03255"
     CDS             561..1847
                     /locus_tag="VNZ84_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HWU66001.1"
                     /translation="MQSTSASCHAMLMAAPASGQGKTMTTAALARAYRRRGLRVQAFK
                     CGPDFIDGMILEVATGKPVYNLDLGMCGEADAQRLLHQAAQNNDVILLEGVMGLYDGK
                     PSCADIAERFGIPVALVINAKAMAQTFAAIAYGLYAFKPALKRAGVIANQVGSDGHAK
                     MIKEALPADIPWLGAMKHDVQLSLPERHLGLHLAQEIADIDLRIEAAAQLLGPDLPLP
                     PAVDFTAPEKQDIPPLLQGKTIAVAKDAAFCFTYQSNLDLLQTLGAKVTFFSPLHDAQ
                     LPSADAYWLPGGYPELHLEQLQHNHSMRESLHATAMAGKPILAECGGMLALGERLNGQ
                     PVFGLLPGHSEIQTKLQGLGTQHATFSSTSDDSASIGAHTFHYGKFETDLPVIAQGKG
                     KYGAGEPVYRQNNITASFLHFYFPSNPPLAAQFFLP"
     gene            1844..2518
                     /gene="bluB"
                     /locus_tag="VNZ84_03260"
     CDS             1844..2518
                     /gene="bluB"
                     /locus_tag="VNZ84_03260"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HWU66002.1"
                     /translation="MSFAYPDADKAAIYKVIAERRDMRHFLPTPIAPELLQKLLQAAH
                     HAPSVGLMQPWRFIRISDTTLRQAIHALVDEERKHTAQAIGEYENTARMAEFMRLKVE
                     GILDCGELLVATLCDQREKYIFGRRTLPEMDLASVSCAIQNLWLAARAEGIGMGWVSL
                     FDPQKLAQLLGMPDGAKPVAILCLGYVNTFYKSPMLVEEGWSTEKPLSDMVMENGWQT
                     DESNKP"
     gene            2515..3318
                     /locus_tag="VNZ84_03265"
     CDS             2515..3318
                     /locus_tag="VNZ84_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HWU66003.1"
                     /translation="MKHWPQRIVCLTTETVEVLYLLGEQDRIVGISGFTTRPAIARKE
                     KPKISAFTSANIEKILALQPDLVLCFSNLQADIAAALIKAGCQVHVFNQRSLDETLQM
                     ILTVGDLVGASDKARQLVEGYQSQLDAARLQAAKLPRKPKVYFEEWDAPMMCSIRWAA
                     ELIEAAGGEDCFPELSQFHSARDRQVTANQVLARQPDIIIGSWCGKKFQPEKVKARPG
                     WQAMPAVSDGHLYEIKSVDILQPGPALFSEGLVQLQTIIHRWQQQGGHS"
     gene            3315..3839
                     /gene="cobU"
                     /locus_tag="VNZ84_03270"
     CDS             3315..3839
                     /gene="cobU"
                     /locus_tag="VNZ84_03270"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HWU66004.1"
                     /translation="MTTHLILGGARSGKSAYAERLASALEYPVTYIATAQVYDDEFAK
                     RVAHHKTRRPPYWGLVEAPFNLGQTLLDNDAPETCLIVDCLTLWLAQCICPDCDKPER
                     LDWQAEKQALLNALPQLQAHVFLVSNEVGMGIVPLGEINRQFQDEQGRLNQHVAAIAD
                     QVSFIAAGLPLKLK"
     gene            3855..4670
                     /locus_tag="VNZ84_03275"
     CDS             3855..4670
                     /locus_tag="VNZ84_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HWU66005.1"
                     /translation="MKKLSLALIVLMTSLAAEAHVPFLKPNQFNVTHHRLTIESAFTE
                     FPFQADFAMDSPNFTITSPSGEVSAIQPIAKTKAAVYLEPTLKVEGTYRISTGQRVGP
                     VYKAVETPDKKLYFAADMKRVAGKPTTMNYYSYADTYIFKGQQNYVAKPFNKGLEIIP
                     LASPNGLLLGGELSFQVLEHGQPVPNARIIVVTDNEHFIKHRIEDLYDVENVRASNLH
                     ANQQGIFSFKPQKAGLNFLFVTVHHQLNPALWESLNASLTLEVNLPVDVQSKP"
     gene            4696..4914
                     /locus_tag="VNZ84_03280"
     CDS             4696..4914
                     /locus_tag="VNZ84_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HWU66006.1"
                     /translation="MPTSTFQLKGEYIALCDLLKLEGVADSGGQGKSMVAEGLIKVDG
                     AIERRKTAKIRAGQVVQAFEQTIKVVAT"
     gene            complement(4911..6362)
                     /locus_tag="VNZ84_03285"
     CDS             complement(4911..6362)
                     /locus_tag="VNZ84_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HWU66007.1"
                     /translation="MVQGTTSDAGKSTLVAGLCRVLYRSGVAVVPFKPQNMALNAAVT
                     ADGGEIGRAQAVQAMACGLPPHTDMNPVLLKPNSDTGCQVIIHGQVVGNQEASTYHDY
                     KKTAMQAVLQSYQRLQQQYDCIMVEGAGSPAEINLRANDIANMGFAEAVDCPVILVAD
                     IDKGGVFAHLVGTLALLSESEQARVVGFVINKFRGDIALLQPGLDWLEERTGKPVIGV
                     LPYMHDLHLEAEDAVAKSNTQKAGVQLTVVVPILPHIANHTDFDALRLHPQVDLKFAH
                     LNAPVPAADLIILPGSKNVRGDLAALREHGWESVIARHLRYGGKVLGICGGFQMLGQA
                     LHDPLAIEGEAGSSAGLGWLDMETTLEPHKQLKQVAGKLAFAEASVQGYEIHMGVSRG
                     PALQHPVHLMDGMAEGARSTDGQVAGSYIHGLFDHPQACAAWLQWAGVSEQVAYDYTQ
                     LREQSLEKLADSVQQHFHWRTLAPYLPKGFAPG"
     gene            6365..7318
                     /gene="cbiB"
                     /locus_tag="VNZ84_03290"
     CDS             6365..7318
                     /gene="cbiB"
                     /locus_tag="VNZ84_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HWU66008.1"
                     /translation="MFSLPAYPCPDYLTLALLACGAVLLDFWLGEPRRWHPLVGFGWL
                     ASQVEKYFNPLPKQAQRSQYLTGVLGVCLVVLPIVMSSLLLMYCSFWLALSLHLYLLY
                     FAIGHRSLRQHGLAVYHALQAGQFQQAQRATAYMVSRDVEAIEPVSATIESLLENGND
                     SVFGALFWFFVAGGPGALTYRLVNTLDAMWGYRTPRYFYFGWAAARLDDVLNYLPARL
                     TALTYALLGHTALAWQSWRAQAPLWDSPNAGPVMSAGAGALNVQLGGRARYHGEWHDR
                     PRLGTHTTPVAEDIPRALQLVSKGMLSWLVVAVLTGALAYA"
     gene            7311..8303
                     /gene="cobD"
                     /locus_tag="VNZ84_03295"
     CDS             7311..8303
                     /gene="cobD"
                     /locus_tag="VNZ84_03295"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HWU66009.1"
                     /translation="MLEHGGNLQQAAQQYGRPLSYWLDVSTGINPQHYPIPALAPALF
                     QRLPPATEHLHAAARTYYGCQHVLACAGSQAALQLLPSLRAPCRVAMPRTMYQEHAYA
                     WQRAGHQVQFFEQTPGQDLLNQSDVLLVCNPNNPTGHLYAPDTLLDWHAQLAARGGWL
                     VVDEAFMDTTPQYSLASHTGQPGLWVLRSLGKFFGLAGLRVGFLLGEPEALTQVESLL
                     GPWTITGPSQTIASQALVDYDWQQQMRTHLPAHSKQLAQLLGLHGLTPSGGTHLFQYV
                     PHQAAWQLQQALAKEGIWTRYFESPSALRFGLPPAGDWARLDQALQHATNQLSG"
     gene            complement(8284..8916)
                     /locus_tag="VNZ84_03300"
     CDS             complement(8284..8916)
                     /locus_tag="VNZ84_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HWU66010.1"
                     /translation="MYIPKNFAAPGKDALDDLIRVYPFATLVMTTATGVEVNHLPLYL
                     DGNGVLTGHLARANPLWQTVQGDTDVLAVFHGPHTYISPSCYPSKVRTGMVVPTWNYV
                     VVHARGKLRVIEDVNWLRAHLEALTAQHEAGFAEPWQLEDAPAGFLDKLMQAVVGIEM
                     TVTELTGKWKVSQNQPLENQMGVMWGLSASDDEYARKMAAVVKVVNHLTG"
     gene            complement(8932..9144)
                     /locus_tag="VNZ84_03305"
     CDS             complement(8932..9144)
                     /locus_tag="VNZ84_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66011.1"
                     /translation="MRAIGIFLLVIVLLPVAYGQYAHRKQAMNEADQTVCVTNMRMAC
                     KSTPEPYQAMVMVRGQLLVLPSSHQE"
     gene            9249..10658
                     /locus_tag="VNZ84_03310"
     CDS             9249..10658
                     /locus_tag="VNZ84_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWU66012.1"
                     /translation="MPVTPPLITLNSQDSVPLVEQIVTGVKQLVEQRVLRSGTRLPSI
                     RDFAIQHGISRFTVVDAYDRLVALGYLLSRRGSGFYVAPLNKPEESVSQTSYLDRADD
                     VLWLLHNTFRDVPDAIRPGCGWLPKDWLDEKVIQKSLNELSRKSGYFLTGYGDVRGYL
                     PLRQQLAQKNLADSGIHAEAKHILMTNGASHGLDLVARLLIKPGDVVLVDDPGYYTLF
                     GFLKSMGARIMGVPRLLNGPDVSVLQQLLQEHRPKLFFTNTVLHNPTGTSTSLSVAHQ
                     ILQLAEKYGLYIVEDDIYGDFHPQNATRLAALDQLNRVIYVSSYSKTISASLRVGFIA
                     CQADLANRLLDLKLLTGLTSSEINQRVMHQILVEGRYRKHMEKVRSQLEVKRTQVIHA
                     LEECGLSIHAEPAGGMFVWAKLANGKNAAEIASEASKLNIMLAPGNLFRPYQEPSSWL
                     RFNVAHCEDGKVFELLKNA"
     gene            complement(10669..11235)
                     /gene="cobC"
                     /locus_tag="VNZ84_03315"
     CDS             complement(10669..11235)
                     /gene="cobC"
                     /locus_tag="VNZ84_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HWU66013.1"
                     /translation="MKLTLVRHTSLQIAEGICYGQSDIDVSINFASELSQLKQKLIHE
                     QVDAVYSSPLQRCSKLAHALGFDGILHDERLKELHFGDWEMQSWNDIPRELFDVWAQN
                     YAELAPPNGETFAGLQQRGIDFLQEVQARHSGQHVLVVTHGGMIRALIAHVLNMQLKG
                     LFRFHVDYASLTRLDLSGAIPKVEFVNR"
     gene            complement(11232..12023)
                     /locus_tag="VNZ84_03320"
     CDS             complement(11232..12023)
                     /locus_tag="VNZ84_03320"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HWU66014.1"
                     /translation="MKLQWHYFLLAVGFFTRLPVPAVADFQESDLNHAARYFPLVGLL
                     VGLLAAVVWWLASWLLPPALAILSSMAATIYVTGAFHEDGLADSADGLGGGMTRDRKL
                     EIMQDSRLGSYGAIALIGMLMFKFQALSALTPAILPFALIAAHALSRLAAVYIMATAS
                     YVRFKGKSKPLATTLTRQDVWCATACGLICWLGFSAMLGVNHSAMAAGRFLLMTALPV
                     FGIWWWWRRLILRQLQGYTGDTLGAAQQLTEVAFYLGLLAWEHLA"
     gene            complement(12050..13096)
                     /gene="cobT"
                     /locus_tag="VNZ84_03325"
     CDS             complement(12050..13096)
                     /gene="cobT"
                     /locus_tag="VNZ84_03325"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWU66015.1"
                     /translation="MQNFQITPIQNEVLQTALIQRINQKTKPLGALGRLESLAIQVGL
                     VQQTEHPLLQQPTMLVFAGDHGIVAEGVSPYPQAVTAQMVLNFLNGGAAINVFARQHG
                     FDLHVIDSGVNAVLQPHPMLIDAKIGMGTVNFLHQPAMTAEQCAQAITRGAQIARDVI
                     AQGCNVLALGEMGIGNTSSASCLMSVLCGLPMHQCVGRGTGLTDKGLHHKLSILTSAL
                     QHHHVSEKEPLHALAVFGGFEIAMMVGALLAAAEARVILLIDGFIATSALLVAAKLYP
                     QVVDYCVFAHCSDESGHSIMLSHLKAQPLLHLSLRLGEGTGAVLAYPLVQSAVAFLNE
                     MASFDSAGVSEQQQ"
     gene            complement(13208..14668)
                     /locus_tag="VNZ84_03330"
     CDS             complement(13208..14668)
                     /locus_tag="VNZ84_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HWU66016.1"
                     /translation="MQSFLQSRVTHWVTVPLAVAALFGAGYWVKGDTLVNRAQATAPV
                     SAKTMAAPSAYITLPNFASIANSQGAAVVNISVTGMVKTAGIPGLDPSDPMFELFRRF
                     QPNLPQTETPMNGLGSGFIVKSDGVILTNAHVVDKAEVVTVKLTDKREFQAKVIGVDK
                     LTDVAVLKIDAKDLPTVNIGSTKNSNVGDWVVAIGSPFGFDNTVTAGIISAKSRSLPD
                     EGYVPFLQTDVAINPGNSGGPLFNLNGEVIGINSQIYSRSGGYQGLSFAIPIDVAMNI
                     EQQLLEKGKVSRGRLGIGVQAINQDLASSFGLKSPSGALVSNIEKNGPADKAGLEPGD
                     VIVTFNGQSIDRSSDLPPMVASVKPGSTVNVEVWRNKQLRKLSVRIDEMQTAQAGPAA
                     LPDSQQPNLGVSVRPLTSQEMAQTQLHQGLLVEEVAKGPAAAAGIQPGDVILAVNGDQ
                     VKDVTQLRDKLTKNGRNVALLVMRGENKIYVPVKIG"
     gene            14871..16013
                     /locus_tag="VNZ84_03335"
     CDS             14871..16013
                     /locus_tag="VNZ84_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HWU66017.1"
                     /translation="MADLPSFLKPYWPRRMTGTLRDYARAAIGAGLGILSTGLISLAA
                     VAGDWHGLVFLIAPMGASAVILFCLPSSPLAQPWAILAGNTLAAIAGVFFTQVLPGQL
                     VFAAALAMFTAIALMFALRCLHPPSGAVALTAVLGGDAIHQLGYGFVWWPVLANSALL
                     VLSAWLYHTLTGYRYPAVHHDHTPTSPTHLHYQFGFKQEDLQAALGQFHEVLDINPEQ
                     LQSLLEKTELIAYQRKLEAIECRQIMRPVPTTFEFGDSLETAWQIILKQAEPAIPVVN
                     KAQLVIGLLSVSDFMRHAKAESYHEVAPALKRLIRWSPTTHSDKPEVVGQIMTTPAIT
                     ISEHMHLMHTIPLMTSHHLQLLPVVDHANKLVGILTQTDVIRALYR"
     gene            complement(16010..>16372)
                     /locus_tag="VNZ84_03340"
     CDS             complement(16010..>16372)
                     /locus_tag="VNZ84_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HWU66018.1"
                     /translation="LGVLIGRLGRSIDRARFLTDVPEEKRERFKDELKIIVRRTKWLR
                     RAIGLATLAALCICVSIASMFVSVETGLRLPHLVMVSFIVSMASLILGLLCFLREIVL
                     ASREVIVPFQKLERPEKH"
BASE COUNT         3963 a         4657 c         4086 g         3666 t
ORIGIN      
        1 cgcaaaaaag cggtcattga cgaaaagatc gagcaggcca atcaggaaaa aggcctgctg
       61 ctgatcctca ctggcaacgg caaaggcaag agctcttccg cctttggcat ggtggcccgt
      121 gcactgggcc acggcatgcg tgtcggcgtc gctcaattta tcaaaggccg ctcagatact
      181 ggcgaagaag cctttttccg tcagcatcct gctgtcacct ggcatgtgct gggcgaaggc
      241 tttacctgga acacccaaga ccgccagcgc gacactgaaa ccgcgatgcg cggctggcaa
      301 attgttgcgc agatgctgca agaccctgcg atcaatctcg tggtgctgga cgaactcacc
      361 tacaccttca aatatggcta cctagatgag caaatggtac tggacgccat cgctaaccgc
      421 ccgcccatgc agcatgtggt gatcactggc cgcggcgctt ctgaggcatt gagaaatgct
      481 gcggatacgg tatcagaact catggacgtc aagcacgcct ggcgcgcagg catcaaagcc
      541 caggccggga tagacctgta atgcaatcca cctcagcaag ctgccatgcc atgttgatgg
      601 cggccccggc atcagggcaa ggcaagacca tgaccaccgc cgccctagcg cgcgcctacc
      661 gtcgccgtgg gctgcgcgtg caggcgttca aatgcgggcc agattttatc gatgggatga
      721 tcctggaggt tgccaccggt aaaccggtgt ataacctcga ccttggcatg tgtggtgaag
      781 cagatgcaca gcgattgctg catcaggcgg cacaaaacaa tgacgtgata ctgcttgaag
      841 gtgtgatggg cttgtatgat ggcaagccct catgtgccga catcgccgag cgttttggca
      901 tcccggttgc gttggtcatc aatgccaaag ccatggcgca gacctttgcc gccattgcct
      961 atggcttata tgcattcaag ccagctctca aacgtgccgg tgtgattgcc aaccaagtcg
     1021 gcagtgatgg ccatgccaag atgattaaag aagctttacc agctgacatt ccctggctgg
     1081 gcgccatgaa acatgatgtc caactgagtt tgccagaacg ccacttgggc ctgcatctgg
     1141 cgcaggaaat cgccgatatt gacctccgca tagaagccgc cgcgcaactg ctggggccag
     1201 acctgccatt gccacctgca gtggatttta cggcgccaga aaaacaagac atcccgccat
     1261 tactgcaagg taaaaccata gccgtggcca aagacgctgc cttctgtttt acctaccagt
     1321 ccaatcttga tttgttgcaa acgcttggtg caaaagtgac ttttttctcg ccattgcatg
     1381 atgcgcaatt accctcagcc gatgcctact ggctacctgg cggttacccg gaattgcatc
     1441 tagagcagct acaacataac cacagcatgc gcgaaagctt gcatgccact gccatggcag
     1501 gaaaacccat attggcagaa tgtggcggca tgctcgcact gggcgaacgc ttgaatggtc
     1561 aacccgtgtt tggtttactg ccgggtcaca gcgagattca aaccaagcta caagggttgg
     1621 gcacacagca tgcaacgttt tcatcaacga gcgatgactc agccagcata ggcgcgcata
     1681 cgtttcacta tggcaaattt gaaaccgacc tgccggtgat tgcgcaaggc aaaggcaaat
     1741 atggcgctgg tgagccggtg tatcgccaga acaatatcac cgccagcttt ttgcattttt
     1801 acttcccgtc caatccaccc ctggcggcac agtttttcct gccatgagtt tcgcctatcc
     1861 ggatgccgat aaagcggcca tttataaagt gattgccgag cggcgcgaca tgcggcattt
     1921 tttgccaacg ccgattgcgc cagaattact gcaaaaactg ttgcaagccg cccatcacgc
     1981 gcccagtgtc ggcctgatgc agccatggcg ctttatccgc atcagcgata ccacattacg
     2041 ccaagccatc cacgcactgg tcgacgaaga acgcaagcac accgcgcaag cgattggcga
     2101 atatgaaaat acggcccgta tggctgagtt tatgcgcctg aaagtcgaag gcattctgga
     2161 ctgtggcgaa ctgctggtag caacgttatg tgaccagcgt gaaaaatata tttttggccg
     2221 ccgtacgttg ccggaaatgg atctggcctc ggtcagctgc gccattcaga atttgtggct
     2281 agccgcgcgc gctgaaggca taggcatggg ctgggtcagc ctgtttgacc cgcaaaaact
     2341 ggcgcaatta ctgggcatgc ctgacggcgc aaaacccgtc gccattttat gcctgggtta
     2401 tgtgaatacg ttttacaaat cgcccatgct ggtggaagaa ggctggtcca cagaaaaacc
     2461 gctttctgac atggtgatgg agaatggctg gcaaacagac gaaagtaaca aaccatgaag
     2521 cactggccgc aacgcatcgt ctgtctgacc acggaaacag tggaagtcct ttatctgctg
     2581 ggcgaacaag accgcattgt cggcatctca ggctttacca cgcgtccggc cattgcccgt
     2641 aaagagaagc caaaaatcag cgccttcacc agcgccaata ttgagaaaat cctggcgtta
     2701 cagcctgacc tggtactgtg cttctccaac ttgcaggctg acattgcggc ggcgctgatc
     2761 aaggccggtt gccaggtaca tgtgtttaac caacgcagcc tggatgaaac cttgcaaatg
     2821 atactcaccg tcggcgattt agtcggtgcc agtgacaaag cgaggcaatt ggtagaaggt
     2881 tatcagtctc aactggacgc ggcacgcctg caagcggcca aattgccacg caaacccaaa
     2941 gtctattttg aagaatggga tgcgcccatg atgtgcagca tccgctgggc agctgaactg
     3001 attgaagcgg cgggcggcga ggattgcttc cctgaattga gccagtttca tagtgccaga
     3061 gaccgtcaag tgacagcaaa ccaagtgttg gccagacagc cggatattat tattggctct
     3121 tggtgcggca aaaaattcca gccggaaaaa gtaaaagcaa gaccaggctg gcaggccatg
     3181 cctgccgtca gcgatggtca cctttacgaa atcaagtcag tggatatttt acaacccggg
     3241 ccggcgttat ttagcgaagg cctggtgcaa ctgcaaacca tcattcatcg ctggcaacaa
     3301 caagggggtc attcatgacg acacacctga ttttaggcgg ggcacgcagt ggtaaaagcg
     3361 cttatgccga acgcctggcc tcggcacttg agtatccagt cacttatatc gccaccgcac
     3421 aagtttatga cgacgaattt gcaaaacgtg tggcacatca taaaacccgc cgtccacctt
     3481 attggggatt ggtcgaagcg ccatttaatc tgggacaaac tttgctagat aacgatgcgc
     3541 cagagacttg cctgattgtg gattgcctga ctttatggct ggcacagtgc atttgccccg
     3601 attgcgataa accagagcgc ctggactggc aggcagaaaa acaggccttg ctgaacgccc
     3661 tgccgcaatt gcaagcccac gtatttctgg tcagcaacga ggttggcatg ggcattgtgc
     3721 cgctgggtga aatcaaccgc cagtttcagg atgaacaagg tcgcttaaac caacatgtgg
     3781 cggccatcgc agatcaagtc agttttattg ctgcaggctt gccgttaaag cttaaataac
     3841 aagaaagcac acgtatgaaa aagttgtccc tcgccctgat cgttttaatg acctcactgg
     3901 ctgccgaggc gcatgtcccg tttttaaaac ctaaccagtt caatgtcacg catcaccgct
     3961 tgacaattga atctgcgttt accgagtttc cgttccaggc tgactttgcc atggactcgc
     4021 ctaacttcac gatcacctca ccgtctggcg aggtctcggc catccagccg atcgccaaaa
     4081 ccaaggctgc ggtatatctg gaacccacac tcaaagtaga aggcacctac cgtatcagca
     4141 cggggcaacg cgtcggtccc gtgtataaag ccgtggaaac gccggataaa aaactctact
     4201 tcgcagctga catgaaacgc gtcgccggta agccaaccac catgaactac tacagctatg
     4261 ccgataccta tatttttaaa ggccagcaaa actatgtggc taaacctttt aacaaaggtc
     4321 tggagattat tccactggcg tcacctaacg gtttattact cggcggtgag ttaagctttc
     4381 aggtgttgga gcatggtcaa cccgtcccca atgcacgtat tattgtggtg acggataatg
     4441 agcactttat caaacaccgc attgaagatt tgtacgatgt agaaaatgtc agggcgtcca
     4501 acctgcatgc taaccagcaa ggtatattta gctttaaacc acagaaagcc gggctgaatt
     4561 ttctgtttgt gacggttcac caccagctta atccagcgtt gtgggagagc ctgaacgcgt
     4621 cgttgacgct ggaagttaat ttaccagtag acgtacaaag caaaccttaa gcgcaagata
     4681 agaaaggaat tctctatgcc cacctcgaca tttcaactca aaggtgaata catcgcgctc
     4741 tgtgacttgc ttaaactgga aggcgttgcg gatagcggcg gtcagggaaa atccatggta
     4801 gcggaaggct tgattaaggt agatggtgcc attgagcgca gaaaaaccgc caaaatccgc
     4861 gccgggcaag tggtgcaggc gtttgagcaa accatcaagg tggttgcgac ttaacctggc
     4921 gcaaaccctt tgggcagata gggcgcaagc gtgcgccagt gaaagtgctg ctgtacactg
     4981 tctgccagct tctccagact ctgctcgcgt aactgcgtgt aatcgtaagc cacctgctca
     5041 gacaccccgg cccattgcaa ccaggcggca caggcttgcg ggtgatcaaa caagccgtga
     5101 atataactac ccgcaacctg gccatcagtt gaccttgcgc cttctgccat gccgtccatc
     5161 aggtgtacag gatgctgcaa ggccgggcca cggctcactc ccatatgaat ctcatagccc
     5221 tgcacactcg cctcggcaaa ggcgagcttg ccagcaacct gcttgagctg cttgtgtggc
     5281 tctaatgtcg tttccatatc cagccaaccc aggccagcac tactgcccgc ttcgccttca
     5341 atcgctaatg gatcatgcaa agcctggccc agcatttgga agccgccgca aatgcccagc
     5401 actttgccgc cgtaacgtaa atggcgcgca atgacggact cccagccatg ctcacgtaag
     5461 gcagctagat ctccgcgtac attcttacta ccaggcaaaa tgattaaatc cgccgcgggc
     5521 acaggcgcat tcaaatgcgc aaacttgagg tctacttgcg ggtgcaggcg taaagcatca
     5581 aagtcggtat ggttagcgat atgtggcaat atgggtacaa ccaccgtcag ttgaacccct
     5641 gctttttgag tattgctttt tgccaccgca tcttccgctt ccagatgcaa atcatgcata
     5701 tagggcaaca cgcctatcac cggcttgccg gtacgctcct ccagccaatc cagtccaggc
     5761 tgcaacaagg cgatatcgcc acgaaattta ttaatgacaa aaccgactac cctggcctgt
     5821 tcgctctcgg acaatagcgc gagtgtgccg accaagtgcg caaatacacc gcctttatca
     5881 atatctgcaa ccagaatcac cgggcaatcg accgcttcgg cgaaccccat attggcaata
     5941 tcgttggcac gcagattaat ctcggcagga ctgcccgccc cctccaccat gatgcagtca
     6001 tattgctgct gtaagcgctg ataagattgc aacacggcct gcatcgcggt ttttttatag
     6061 tcgtggtaag tactcgcttc ctgattaccg accacctggc cgtgaataat cacctggcag
     6121 cccgtgtcag aattcggctt aagcaacact gggttcatat cggtatgcgg aggcaatccg
     6181 caggccatcg cttgcaccgc ctgcgctctg ccaatctcac cgccatcagc ggtcacggcg
     6241 gcattgagcg ccatgttttg cggcttgaat ggcaccactg ccacgccact gcggtacaat
     6301 acgcggcaca atccggccac cagcgtactt ttaccggcat ccgacgtggt gccctgaacc
     6361 atcaatgttt tcactgcctg cttatccatg ccccgattat ctcacactcg ccctgcttgc
     6421 ctgtggcgcg gtgctgcttg atttctggct gggtgaacca cggcgctggc atccactggt
     6481 cgggtttggc tggcttgcct cccaagtgga aaaatacttt aatcctttac ccaaacaagc
     6541 ccagcgcagc caatatctca ccggcgtgct gggcgtctgc ctggttgtat taccaattgt
     6601 catgagcagc cttctactca tgtactgttc attttggctg gcattatcat tacacctcta
     6661 tctcctgtat tttgccatcg gccaccgtag cttgcgccag catggcctgg ccgtttatca
     6721 cgctttgcag gcgggccagt ttcaacaggc acagcgggcc accgcctaca tggtcagccg
     6781 ggatgtggaa gccatagaac ccgtcagcgc caccatagag tctttgctcg aaaacggcaa
     6841 cgacagtgtg tttggcgcgc tgttctggtt ttttgtcgca ggtggtcctg gcgcgttgac
     6901 ttatcgcctg gtcaacacgc tggatgccat gtggggctac cgcactccac gttatttcta
     6961 ttttggctgg gccgctgccc ggctcgacga tgtgctcaac tatcttccag cccgcctgac
     7021 tgcgctgact tacgccttgc ttggtcatac agcgcttgca tggcagtcct ggcgcgcgca
     7081 ggcgccatta tgggatagcc caaacgctgg gccggtcatg tccgcaggcg caggcgcgct
     7141 gaatgtgcaa ttgggcggtc gcgcccgcta tcacggtgag tggcatgacc gccctcgcct
     7201 gggcacccat acaactccgg tagctgaaga tattcccagg gcattgcagt tggtgagtaa
     7261 gggcatgctg agctggctag tcgtggctgt attaactgga gcgttggcct atgcttgagc
     7321 atggcggcaa tttgcagcaa gccgcgcagc aatacggccg gccactctca tactggctgg
     7381 atgtatcgac cggtatcaat ccgcaacact acccgattcc ggcattggcg cctgcactgt
     7441 ttcaacgcct gccaccagcc accgagcacc tgcatgcggc cgcacggacg tattacggct
     7501 gccagcatgt gctggcctgt gcaggctcac aggctgcctt gcaactattg ccaagcttgc
     7561 gtgcgccttg ccgcgtggcg atgccgcgca ccatgtatca ggagcatgca tatgcctggc
     7621 aacgcgccgg acatcaggta cagttttttg agcagacacc gggtcaagac ctgctcaatc
     7681 agtcggatgt attactggta tgcaacccca ataacccgac cgggcatcta tatgctccag
     7741 acaccttgct agactggcat gcgcaactgg ccgcccgggg tggttggcta gtcgtcgacg
     7801 aagcgtttat ggacacaaca ccgcagtaca gtctcgccag ccataccgga cagccagggt
     7861 tatgggtatt gcgatcactg ggaaaatttt tcgggctggc aggactgcga gtgggcttct
     7921 tactcgggga gccagaggcc ctgacacaag tggaatcatt acttggccca tggaccatca
     7981 ccggccccag ccaaacgatt gccagccagg ccttggtcga ctatgactgg caacaacaga
     8041 tgcgcacgca tctgccagca cattctaagc aattagcgca attacttggc ctgcacgggc
     8101 tcacacccag tggcggtacc catttattcc aatatgtgcc gcatcaagcc gcttggcagt
     8161 tgcagcaagc cctagccaaa gaaggcatct ggacgcgtta ttttgagtca ccctctgcac
     8221 ttcgttttgg cttgcctcca gcaggtgact gggcgcggct agaccaggca ttacaacacg
     8281 ccactaacca gttaagtggt taacgacctt gaccaccgcg gccatttttc gggcatactc
     8341 atcgtcactc gcactcaagc cccacatcac gcccatttga ttttccagtg gctgattttg
     8401 actcaccttc catttgccgg tgagctcagt gaccgtcatt tcaatcccaa ccaccgcctg
     8461 catcaacttg tcaagaaagc cagcaggcgc atcttcaagt tgccaaggct ctgcaaagcc
     8521 ggcttcatgc tgggccgtaa gggcctccag atgtgcgcgt aaccagttca catcctctat
     8581 gacccgcaac ttgccacgcg catgtaccac cacataattc caggtgggta cgaccatgcc
     8641 tgttctgacc ttgctggggt agcacgaagg actaatatac gtatgcggcc catgaaaaac
     8701 agccaagaca tcagtgtcgc cttgaaccgt ttgccatagc ggatttgctc gcgccagatg
     8761 accagtcagc acaccatttc catccaaata caacggcaga tgattcactt ccacacccgt
     8821 cgctgtcgtc atgaccaatg tggcgaaggg atagacacgg atcaggtcat ccaacgcatc
     8881 cttacctgga gccgcaaaat ttttggggat atacatagca aaaagctgat ttcactcttg
     8941 atgactggag ggcaaaacaa gtaactgccc cctgaccatg accatcgcct gatagggttc
     9001 aggtgtagac ttgcacgcca tacgcatatt ggtcacacac actgtttgat cagcttcgtt
     9061 catagcttgc ttgcgatggg catattggcc ataggctaca ggtagcaata ctatgacgag
     9121 taaaaatatt ccgatggcac gcattgcata tcccttcccg gttgaatcat tgcatcttac
     9181 tgatcaatac ctgtacacta catatacaat ctaaagatca atatagacac tgtacaggtc
     9241 aaaacaccat gccagttacg ccaccactga ttaccctgaa ctcccaagat tcggtgcctc
     9301 ttgtcgagca aatcgtcacc ggcgttaaac aactggtgga gcagcgtgtg ttgcgcagtg
     9361 gtacgcggtt accgtctatc cgcgactttg ccattcagca tggcattagc cgcttcacgg
     9421 tagtggatgc ttatgatcgc ctggtggcat taggctacct gctgtcacga cgtggctccg
     9481 gcttttatgt cgccccgctt aacaagccag aagaaagtgt ctcgcaaacc tcttacctgg
     9541 acagagcaga cgatgtgcta tggttgctgc ataacacatt cagggatgtg ccagatgcca
     9601 tccggcctgg ttgtggttgg ttacccaagg actggctgga tgaaaaggtc attcaaaaaa
     9661 gtctgaatga actatcgcgt aaatcggggt attttttaac gggctacggc gatgtgaggg
     9721 gatatttacc gctacgacaa cagcttgcac aaaaaaacct ggccgacagc ggcattcatg
     9781 ccgaagccaa gcacatcctc atgaccaatg gcgcgtcgca tggcctggac ctggtggcac
     9841 ggctattgat taagccaggg gatgtcgtgc tggtagatga tcctggctac tacaccctgt
     9901 ttggcttcct gaaatccatg ggcgcccgca ttatgggtgt gccgcgtctg ctcaatggcc
     9961 cagatgtcag tgtcttgcag cagctattgc aagaacatcg gcctaaacta tttttcacca
    10021 acactgtttt acataacccc accggcacca gtaccagctt gtcagtcgcg caccagatac
    10081 tgcagctggc agaaaaatat ggcttgtaca ttgtggaaga tgatatttac ggtgattttc
    10141 atccgcagaa tgccacgcgc ctggcggcac tcgaccagtt aaaccgtgtc atttacgtca
    10201 gcagttactc caagaccatt tcggccagtt tgcgcgtggg attcattgcc tgccaggccg
    10261 accttgccaa caggttgctc gacctgaaat tattgacggg gctaaccagt tccgaaatca
    10321 accagcgtgt gatgcatcaa atcctggtgg aaggccggta ccgaaaacat atggaaaagg
    10381 tccgtagcca gctagaggtc aaaagaacac aggtgataca cgcactggag gaatgtggat
    10441 taagcataca cgcagaacct gccggtggca tgtttgtttg ggcgaaactg gccaatggca
    10501 aaaacgcagc agaaatcgcc agtgaagcat cgaagctaaa tatcatgctg gcacctggca
    10561 acctgttccg tccctatcag gaaccttcat catggctgcg ctttaatgtg gcgcattgtg
    10621 aagatggcaa ggtattcgag ttattgaaaa acgcctaacc cgacaaggtt aacggtttac
    10681 aaattcaacc ttgggaatcg ctcccgaaag atccaaccgc gtcaggctgg cgtaatccac
    10741 atgaaagcgg aacaagcctt tgagttgcat attgagcacg tgtgcgatca aggcccgtat
    10801 catgccgcca tgggtcacaa ccaatacatg ctgaccgcta tgacgtgcct gtacttcctg
    10861 taaaaaatca atgccacgct gctgcaaccc ggcaaaagtt tcgccattgg gcggggccag
    10921 ctcggcatag ttttgcgccc acacatcaaa cagttcacgc gggatatcat tccaggactg
    10981 catttcccaa tcgccaaaat gcagttcctt caggcgctca tcatgcaaaa taccatcaaa
    11041 cccgagtgca tgcgccagct tgctgcagcg ttgcagtgga ctgctataaa ccgcatccac
    11101 ctgctcatga atcagttttt gtttgagctg cgacagctca ctggcaaagt taatcgaaac
    11161 gtcgatgtca ctctggccgt aacagatacc ttcagcaatt tgcaaactcg tatgccggac
    11221 taaagtcagc ttcatgccaa atgctcccag gccagcaagc ctaaataaaa agccacttca
    11281 gtcaattgct gggctgcccc cagtgtatcg ccggtatagc cttgtaactg acgcaggatc
    11341 aggcgtcgcc accaccacca aataccgaac actggtagcg ctgtcatgag cagaaacctg
    11401 ccagcggcca ttgccgagtg attcacaccc aacatggctg aaaagcctag ccagcatatg
    11461 aggccacagg cagtggcaca ccaaacatcc tgtcgcgtca aagtggtcgc cagtggcttg
    11521 gacttgcctt taaaacgtac ataactcgcc gtcgccataa tgtacaccgc tgccagccgg
    11581 ctcagggcat gtgccgcaat caaggcaaac ggcaagatcg ctggcgtgag tgctgacaag
    11641 gcctggaatt tgaacattag catgccaatc agcgcaatcg cgccgtagct gcccaggcgc
    11701 gaatcctgca taatctccag cttacggtcg cgggtcatgc cgccgccaag gccatccgca
    11761 ctgtcggcca ggccatcttc gtgaaaagca cccgtcacat aaatcgttgc cgccatgctg
    11821 cttaaaatcg ctaacgcggg cggcaatagc caacttgcca gccaccaaac caccgcagca
    11881 agcaggccga caagcaggcc gacaagcggg aaataccttg ccgcgtgatt gaggtcagac
    11941 tcctgaaagt ctgccactgc tggcaccggt agtcgtgtaa aaaaaccgac tgccagcaaa
    12001 aaataatgcc attgcagctt cacgggactg accctcacgt cactacccgt cactgctgtt
    12061 gctcactgac gccagcgctg tcgaagctgg ccatttcatt gaggaaggcc acggcggact
    12121 gcaccagagg ataagccagc acagcaccgg tgccttcacc caggcgcagg gataaatgca
    12181 gcaatggctg cgccttgagg tgcgacaaca taatgctatg cccgctttca tcagagcaat
    12241 gggcaaatac gcaataatcc accacttgcg gatagagttt agctgctacc agcaacgcag
    12301 aggtggcaat aaaaccatct atcagcaaaa tcaccctggc ttcagccgcc gccagcaatg
    12361 cccccaccat catggcgatt tcaaagccac caaatactgc gagggcgtgc aatggctctt
    12421 tttccgacac atggtgatgt tgcagggcag aggtcaggat agacagcttg tgatgcaagc
    12481 ccttgtcagt caaaccagtg ccacgaccaa cacactggtg catgggcaag ccacacaaca
    12541 cgctcatgag acagctggca ctggaagtat tgcctatgcc catttcaccc aatgccagca
    12601 cattacagcc ttgagcaatg acgtcacgcg cgatctgtgc accgcgtgta atggcttgcg
    12661 cgcactgctc ggccgtcatg gcgggctgat gcaggaagtt cactgttccc atcccgatct
    12721 tggcatcaat cagcatcgga tgtggctgca acacggcatt cacgccacta tcaatgacat
    12781 gcaagtcaaa tccatgttgg cgcgcgaaca cattaatcgc cgcgccaccg ttcaggaagt
    12841 tcagcaccat ttgcgcggtc actgcctgcg ggtaagggct gacaccttcc gccacaatgc
    12901 catgatcgcc tgcaaatacc agcatggttg gttgctgcaa cagcggatgc tcggtttgct
    12961 gcaccagccc aacctgaatg gccaggcttt ccagccgccc gagggcaccc aggggtttgg
    13021 ttttttggtt gatgcgttga atgagtgccg tttgtaagac ctcattctgg ataggcgtaa
    13081 tctggaaatt ttgcatattg ttattttacc gatcaaaact gaagcgtatc agaattatct
    13141 gttgactcac ctataaaaaa gccccgctat cgcggggcag ggctctaagg atcacacacg
    13201 caaaaaatca accaatcttg accgggacgt agattttgtt ttcaccccgc atgaccagca
    13261 atgcgacatt tctaccgttc ttggtcaatt tatcccgtag ctgcgtcaca tctttcacct
    13321 gatcaccatt cacagcgaga atcacgtctc caggctggat accggcagct gcagccgggc
    13381 ccttcgcgac ctcttcaacc aacaaccctt gatgaagctg tgtttgcgcc atctcttgcg
    13441 aagtcagcgg ccgtacactg acccccagat taggctgctg gctatccggc aaagcggcag
    13501 gcccggcttg cgccgtttgc atttcatcaa tgcgcaccga gagtttcctc aactgcttgt
    13561 tacgccaaac ttcaacattg acggtactgc ccggtttcac actggcgacc atgggtggca
    13621 aatcactaga acggtcgatg ctctgaccat tgaatgtcac aatcacgtca ccaggctcca
    13681 aacctgcttt atccgccggg ccatttttct caatattgct taccagtgct ccactgggtg
    13741 acttcaagcc aaacgagctg gccaaatcct ggttgatcgc ttgtacacca atccccaatc
    13801 gaccacggct caccttacct ttttccagca gttgctgttc aatattcatg gccacatcaa
    13861 tcgggatcgc aaaagacaat ccctggtaac caccgctacg gctataaatt tgcgagttaa
    13921 tgccaatcac ctcaccgttc agattgaaca atgggccgcc agagttaccg gggttaatgg
    13981 caacatcggt ttgcaaaaag gggacatagc cttcatcagg cagggaacgt gacttcgccg
    14041 aaataatgcc tgcggtcacg gtgttgtcaa aaccaaacgg agaaccaatc gcaactaccc
    14101 agtcaccgac attactgttc ttggtgctgc cgatattcac cgttggtaag tccttggcat
    14161 caatcttcaa tacagccaca tccgtcaatt tatcgacccc gatcaccttg gcctggaact
    14221 cacgtttatc tgtcagtttc acagtcacca cttccgcttt gtcgaccacg tgtgcgttgg
    14281 tcaggatcac accatcagac ttgacgataa atccagaacc gagaccattc ataggcgttt
    14341 ccgtttgtgg caggttaggc tggaaacggc ggaataattc aaacatcggg tcgctggggt
    14401 ctagaccagg gatccctgcg gtcttgacca taccggtcac actgatattc accactgccg
    14461 caccctggct attggcaata ctggcaaagt taggcaaggt gatgtacgca ctcggggcgg
    14521 ccatggtttt tgccgagaca ggtgcagtcg cctgcgcgcg attgaccagc gtatctcctt
    14581 tgacccaata ccctgcgcca aacaaggcag ccacggcgag gggaacagtc acccaatggg
    14641 taactctgga ctgtaaaaat gattgcatca cagtttcctt tcttgcgtat caatccattc
    14701 atcccgcgat caatccacgg gtttctgcgg atatgagcga taaaaccgcc atgaagtttc
    14761 actgtttttg tatcttaatt gtatccgcag gcatctcagt tctagtaccg gctcccaacc
    14821 agacaaaggc ttgcgtgata cactgcgtct tttgtttttt gacctgagtc atggcagatt
    14881 taccgtcttt tctgaagcct tactggccac gtcgcatgac cggcacgctg cgcgattacg
    14941 ctcgggctgc gattggtgcc ggactaggta tcttgtccac cggcctgatt tcactggcag
    15001 ctgttgccgg tgattggcat ggcttagtct tcctgattgc acccatgggc gcttcagccg
    15061 tgatcctgtt ttgcttgccc tcaagcccac tcgcccaacc ctgggccata ttggccggaa
    15121 atacacttgc cgcaatcgcg ggcgtatttt ttacccaagt gctgcctggc cagctggtat
    15181 ttgcggctgc cttggccatg ttcacggcga ttgcactgat gtttgcctta cgttgcctgc
    15241 accctcccag cggcgcagtt gcgttgactg ccgtgttagg tggagacgct atccaccaac
    15301 tgggctatgg ctttgtctgg tggccggtat tggccaatag tgcattactg gttttgtccg
    15361 cctggctcta tcacacgttg actggctacc gttacccggc cgtgcatcat gaccatacgc
    15421 cgacatcgcc aacgcatctg cactaccagt tcggcttcaa acaggaggat ctacaagccg
    15481 cgctgggcca gttccatgaa gtactggaca tcaaccctga acagttacaa agcctgctgg
    15541 aaaaaaccga actgattgcc tatcagcgca aactggaagc catcgagtgc cggcaaatca
    15601 tgcgtcctgt gccaaccacc ttcgagtttg gagattcact ggaaacggcc tggcaaatca
    15661 tcctcaaaca ggcagaacct gccatcccgg tagtcaacaa ggcccagtta gtgatcggtt
    15721 tactaagcgt gtccgacttc atgcgacatg ccaaggctga aagctaccac gaagtggcgc
    15781 ccgcactcaa acgcctgata cgctggtcac caaccacgca cagtgacaaa cccgaggtgg
    15841 ttgggcagat catgaccaca cctgccatca ccatctctga gcatatgcat ctcatgcata
    15901 ccattccgct gatgaccagc catcatttgc aattgctgcc ggtggtcgac cacgccaata
    15961 aactggttgg catcctcacg caaaccgacg tgatacgtgc cctttaccgc tagtgttttt
    16021 cgggcctttc caatttctgg aaaggcacaa tcacttcgcg cgaggccagt acaatctctc
    16081 gcaagaaaca caagaggccg aggattaatg aggccatcga aacaataaaa gagaccatga
    16141 ccaggtgcgg taagcgcaga ccagtctcca cggagacaaa catactggca atcgagacac
    16201 aaatacacaa cgccgcaagc gtagccaggc caatggcccg ccgcaaccat ttggtacggc
    16261 gcacaatgat tttcaactca tctttaaagc gctcacgctt ttcttctggc acatccgtca
    16321 agaatcgggc gcgatctatc gatcgcccta aacgcccgat caataccccc ag
//
LOCUS       DATMSK010000096         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_265634,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000096 DATMSK010000000
VERSION     DATMSK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265634"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /gene="lipA"
                     /locus_tag="VNZ84_03345"
     CDS             <1..804
                     /gene="lipA"
                     /locus_tag="VNZ84_03345"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWU66019.1"
                     /translation="QVLRDNNLHTVCEEASCPNIGECFSGGTATFMILGDICTRRCPF
                     CDVAHGKPLPPDPKEPENLARTIAQMKLRYVVVTSVDRDDLPDGGAQHFVDCIRAIRE
                     QSPDIKIEILVPDFRGRLDVSLKILREAPPDVMNHNLETVPRLYKQARPGSDYQHSLN
                     LLKEFSHMYPDVPTKSGLMLGLGETDEEILEVMRDLRAHNVSMLTLGQYLQPSVHHLP
                     VLRYVTPDTFAWFKQQADAMGFDNAASGPMVRSSYHADQQAHAVIEQQA"
     gene            860..2170
                     /gene="gshA"
                     /locus_tag="VNZ84_03350"
     CDS             860..2170
                     /gene="gshA"
                     /locus_tag="VNZ84_03350"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWU66020.1"
                     /translation="MVPHLTTALSGPLVPLEKRMLDAMPDIEHWFRTQWQEVAAPFYA
                     SVDLRNAGFKLAPVDTNLFPGGFNNLNPEFLSLSVQAAQVAVEKICPDARRLLLIPEN
                     HTRNTYYLRNVVELCNILKAAGMEVRVGSLSPEITEITRLETHDGLPLILEPLVREKN
                     RLQLKAVSFEGVDYPAFDSCAILLNNDLSGGVPDMLQNLEQTLIPPLHAGWFTRRKSQ
                     HFAAYDKVVAEFAKVLNIDPWMLNPYFDTASGIDFHARVGEDQLADKVDAMLKKIQAK
                     YDEYDIKHEPFVIVKADAGTYGMGIMSVKSADEVRDLNRKTRNKMSVIKEGMEVSEVM
                     IQEGVYTFENINDAVAEPVVYMMDHFVIGGFYRVHTSRGIDENLNAPGMHFAPLAFEK
                     PCTLPDCAGAPDAPPNRFYAYGVVARLALLAAGMELQENDPLNA"
     gene            2189..3139
                     /gene="gshB"
                     /locus_tag="VNZ84_03355"
     CDS             2189..3139
                     /gene="gshB"
                     /locus_tag="VNZ84_03355"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HWU66021.1"
                     /translation="MKLLFILDPLDALVTYKDTSLAMMRAAQARGHELWVCEQHDWQL
                     QHDVVTVDAQTFAFNIDGSWKTSDKSTVSPADFDAVLMRKDPPFDNEYLYSTYLLELA
                     QQQGARIINDPASIRGWNEKLSVARFPQFAPPFIVTSQQDKILAFLGEHGDIIVKPLD
                     GMGGSGIFRLRKDDPNTYAILETLTQFQTQTIMVQRYIPDIVKGDKRILVINGEPLPY
                     ALARIPKTGETRGNLAAGGKGVAQPLSPRDKEIAETVGKILKQHGLFLVGLDVIGDYL
                     TEVNVTSPTGMVEIAAQSKDWTPSCDPAMMMIEALEQLVA"
     gene            3242..4546
                     /locus_tag="VNZ84_03360"
     CDS             3242..4546
                     /locus_tag="VNZ84_03360"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HWU66022.1"
                     /translation="MRVLVLGSGVIGVTSAYYLALKGFDVTVIDRQPAVGLETSFANA
                     GQISPGYSAPWAGPDVPLKALKWLFQRHAPLSLRPDFSLWQLQWIAHFLRNCSLERYE
                     LNKSRMVNLAEYSRDCLRALRVSTGIQYEQRSRGTLQVFRTQKQLDAEAKDIAVLSHM
                     GVPFQHVDSEGCARIEPALTPVKGQLLGGLHLPDDETGDCQLFTTQLALEATKLGVQF
                     RQNTHIQRLLIQKNHLLGVEVHTGHEHIETLEADHYVIALGSYSRQLMQTMGLSLPVY
                     PVKGYSLTVPIINSDAAPVSTVMDETYKVAITRFDQRIRVGGMAELAGFNLNLNPCRR
                     DTLEMVLNGLFPQAGDSSQAQFWTGLRPMTPDGTPIIGQANSAYPNVWLNTGHGTLGW
                     TMACGSGQVLADLIAHDQPAIETQELSYKRYFSSRLPSRQTV"
BASE COUNT         1216 a         1303 c         1228 g         1141 t
ORIGIN      
        1 caggtactgc gcgacaacaa cctgcacact gtgtgtgaag aggccagctg ccccaatatt
       61 ggtgaatgtt ttagcggtgg cacggccacc ttcatgattt tgggtgacat ttgtacccgc
      121 cgctgcccgt tctgcgatgt ggcgcacggt aagccactgc cgccagaccc gaaagaaccg
      181 gaaaacctgg cacgcaccat tgcccagatg aaattgcgtt atgtggtcgt cacgtccgta
      241 gaccgcgatg atttacctga cggcggtgcc cagcattttg tcgactgcat ccgtgccatc
      301 cgcgaacaat ctcccgatat taaaatcgaa atcctggtgc cggatttccg tggccgcctc
      361 gacgtttcac tgaaaatctt gcgtgaagcg ccaccagatg tcatgaacca taacctggaa
      421 accgtgcccc gcctgtataa acaggcgcgc ccgggctctg attaccagca ttcgctcaac
      481 ctgctcaaag aattcagcca tatgtatccg gatgtcccaa ccaaatcagg tttgatgttg
      541 ggcttaggcg aaaccgatga agagatactg gaagtcatgc gcgacttgcg tgcacacaac
      601 gtgagtatgc tgacactagg gcaatacctg caacccagcg tgcaccattt accagtacta
      661 cgctacgtca caccagatac gtttgcctgg tttaaacagc aagccgacgc tatgggcttt
      721 gacaatgccg ctagcggccc tatggtgcgc agtagttatc atgcggatca gcaggcgcac
      781 gccgtgattg agcaacaagc ataattgaaa gacagcgcca acctaccggc ctgaataaaa
      841 acaaatacag gagcaaatga tggttcccca tttaacgacg gctttgagtg gtccactagt
      901 cccgctggaa aagcgcatgc tggatgccat gccggatatt gaacactggt tccgtacgca
      961 atggcaggaa gtcgccgcac cgttttatgc cagcgtcgat ttacgtaatg ccggctttaa
     1021 attggcccct gtagatacca acctgtttcc aggcggtttc aataacctga atcctgagtt
     1081 ccttagcctg tctgtgcaag cagcccaggt cgctgtcgaa aaaatctgtc cggacgcacg
     1141 ccgtttattg ctgattccgg aaaaccatac ccgcaacacc tactatctgc gcaatgtggt
     1201 cgagctctgt aatatcctca aagccgcagg gatggaagta cgtgttggta gcttgtcacc
     1261 agaaattacc gaaatcacaa gactggaaac gcatgatggc ctgccactga tattggaacc
     1321 attagtgcgt gaaaagaatc gcctgcaatt aaaagcggtc agttttgaag gcgtggatta
     1381 tccggcgttt gatagctgtg ccattctgct caacaatgat ttatcaggtg gtgtacctga
     1441 catgctacaa aatctggagc agacgttaat tccaccactg catgccggtt ggtttacccg
     1501 tcgcaagtcg cagcattttg ccgcctatga caaagtggtc gctgaatttg ccaaggtact
     1561 gaatatcgac ccatggatgc tgaatcccta ttttgacact gccagtggaa ttgattttca
     1621 tgcccgtgtc ggtgaagacc agctggccga taaagtcgat gccatgctga agaaaatcca
     1681 ggccaagtac gacgagtacg atatcaaaca tgagccattt gtgattgtga aagccgatgc
     1741 cggcacttat ggcatgggga ttatgagcgt gaaatcagca gacgaagtgc gtgatctcaa
     1801 ccgtaaaacc cgcaacaaaa tgtcggtgat taaagaaggc atggaagtct ctgaggtgat
     1861 gattcaggag ggcgtgtata ccttcgagaa tatcaacgat gcagtagctg agcctgtcgt
     1921 gtacatgatg gatcactttg tgattggtgg tttctaccgc gtgcatacca gccgtgggat
     1981 tgatgaaaac ctgaatgcgc caggcatgca ctttgcgccc ttggcctttg aaaagccctg
     2041 taccctgccc gactgtgccg gcgcgcccga tgcaccgcct aaccgctttt atgcttatgg
     2101 cgtggttgcc cgcctggcgc ttttggctgc tggcatggag ctgcaagaaa atgacccatt
     2161 aaacgcataa gcacccttgg gacacatgat gaaactgttg tttattctgg atccactaga
     2221 cgctctggtg acttataaag ataccagcct cgcgatgatg cgcgctgcgc aggcgcgtgg
     2281 ccatgagctc tgggtatgtg agcaacacga ctggcagtta cagcacgatg ttgtcactgt
     2341 ggatgcacag acttttgcat tcaatatcga tggcagctgg aaaaccagtg ataaatccac
     2401 cgtctccccg gccgattttg acgcggtgct tatgcgtaaa gacccgccat tcgataatga
     2461 atacttgtac agtacttacc tgcttgaact tgcgcaacaa cagggtgcac ggattatcaa
     2521 tgatccggcc tctatccgcg gctggaacga aaaactgtcg gtagcccgtt ttccacaatt
     2581 tgcgccaccg tttatcgtca ctagccaaca agacaaaata ctagccttcc tgggcgagca
     2641 tggcgatatt atcgtcaaac ccctggacgg catgggcggc agtggcattt tccggttacg
     2701 taaagatgat cctaatactt acgccatcct ggaaacactc acgcaatttc aaacgcaaac
     2761 catcatggtc cagcgctata tccccgacat tgttaaaggg gacaagcgca ttttggtcat
     2821 taatggcgaa ccattacctt atgcactggc gcgcatccct aaaacggggg aaactcgcgg
     2881 caatctggcc gcaggtggca aaggcgtggc tcagcccctg agtccacgtg acaaggaaat
     2941 tgctgagact gtgggtaaaa tactcaagca gcacggcctg tttttagtcg ggctggatgt
     3001 gattggcgac tacctgactg aagtcaatgt gaccagcccg accgggatgg tggaaattgc
     3061 agcgcaatcg aaagactgga cgccaagctg cgacccagcc atgatgatga ttgaagcact
     3121 ggagcaactc gttgcttaaa ctcaactaac tccactcgcc agattcagtc tgcaagattt
     3181 tagctttgtc atgtgactag tatcgagtaa tctaacagcg atgctgccgt aggagtatgt
     3241 catgcgcgtg ttggtgctgg gttctggtgt cataggggtc acaagtgctt attacctggc
     3301 attaaaaggc tttgatgtca ccgtcattga ccgccagcct gctgtcggcc tcgaaaccag
     3361 ttttgccaat gctggccaga tctcgccggg ctactcagcg ccgtgggctg ggcctgatgt
     3421 gccgctcaag gccctcaaat ggttgttcca gcgtcatgca ccgttatccc tccgaccaga
     3481 cttcagtcta tggcaattgc agtggatcgc acacttttta cgtaattgct cgctggagcg
     3541 ttacgagctt aataaatccc gcatggtaaa cctggccgaa tatagccgcg attgcctgcg
     3601 tgcactacgc gtcagtaccg gcatccagta cgaacagcga agtcgcggca cattgcaagt
     3661 cttccgtacc cagaaacagc tcgatgctga agccaaggat atcgccgtcc tgagccatat
     3721 gggcgtgcct ttccagcatg tagactccga gggttgtgcg cgcatagaac ccgccttgac
     3781 cccggttaaa ggccaactgc tgggtggatt gcacttgcct gatgatgaaa ctggtgactg
     3841 ccagcttttt acaacgcagc tggcgctaga agcaacaaag ctgggcgtgc aattcaggca
     3901 aaacactcac atccagcggc tgctcataca aaagaaccac ctgcttggtg ttgaagtaca
     3961 caccggacat gagcatattg aaacgctgga agcagatcat tatgtgattg cactcggtag
     4021 ttattcacgc cagttgatgc aaacgatggg gttaagccta ccggtctatc ccgtcaaagg
     4081 ctattcgctc accgtgccca tcattaacag cgatgccgca cctgtctcca cagtcatgga
     4141 tgaaacctat aaagtcgcca tcacccgctt tgaccagcgc atccgtgtag gcggcatggc
     4201 cgagctggct ggctttaact taaacctgaa tccctgtcgt cgtgacaccc tggaaatggt
     4261 attaaacgga ctattccctc aagctggcga tagttcacaa gcccagttct ggaccggcct
     4321 gcgcccgatg acaccagacg gcacacccat catcggtcaa gccaatagcg cttatcccaa
     4381 tgtatggctg aatacgggtc atggcacctt gggttggacc atggcctgcg gttccggcca
     4441 ggtactggcg gacctgatag cgcatgatca accggccatt gaaacgcaag aattaagtta
     4501 caaacgctac ttttcttcac gcctgccctc tcgccaaacc gtgtaaaaat tcctttattg
     4561 tcatcactct ttcaatatcc tttaaacgat attccttaac gggtatagcg atgctaaact
     4621 ccacgccttg cccgcgctcg tctgaagagt gacaagcacc tgaagagaac cctgtttatc
     4681 acgagaggac aaccttagtg aacaaattga acgatctagc tgcatgcacg cgcatggcag
     4741 aaacaccata ttttcctcgc gtgaaaacac tggttgtggc cttgggtctg gctatgttgc
     4801 actcaacttt caccgcttgc gctgacagcg acgccgacgc cgacgcccgc attgcgcagt
     4861 tacagcgcat gcttgaagcg caacagca
//
LOCUS       DATMSK010000097         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_171193,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000097 DATMSK010000000
VERSION     DATMSK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171193"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..616)
                     /locus_tag="VNZ84_03365"
     CDS             complement(122..616)
                     /locus_tag="VNZ84_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWU66023.1"
                     /translation="MHCGKPLSSGFVLIGMFMLIMLSGLVMAQASMRWQTQVLREREH
                     ELIKVGLAYRDAIGRYYNQTPGVVKTYPPTLEALYEDKRFPTARRHLRKLYEDPITHR
                     EGWGIVEAPSGGIMGIYSLSDKPPFKSKGYRGNLEHLNNKRYYGEWYFVYLPQPISSN
                     AASM"
     gene            complement(603..974)
                     /locus_tag="VNZ84_03370"
     CDS             complement(603..974)
                     /locus_tag="VNZ84_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWU66024.1"
                     /translation="MKRGFTLIEMLVVLAILAMLLTIVSPKFMHMLQRAKETSLRHDL
                     LTMRDAIDKFYSDKNRYPETLEELVERQYLKSIPPDPITDRADTWVITLPPNIEEEGS
                     VFDVHSGSALLSEDGTPYALW"
     gene            complement(977..1459)
                     /locus_tag="VNZ84_03375"
     CDS             complement(977..1459)
                     /locus_tag="VNZ84_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWU66025.1"
                     /translation="MTGRVRRKTQGFTLVELLVTLALLALLVTSASPVVQLNAKREKE
                     RELKRALWQIRDALDAYKDAVDQGLIKKKADGSGYPPNLAILVQGVENAQDPKKKKLF
                     FLRRIPRDPFAADSSQTNAETWRIRAYDGALDDMATDVYDVSSRSPAMGINQQPYSEW
                     "
     gene            complement(1472..3427)
                     /locus_tag="VNZ84_03380"
     CDS             complement(1472..3427)
                     /locus_tag="VNZ84_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HWU66026.1"
                     /translation="MNHKNWVWACALPWLVSCSSFSPTGLGPENPAGDATLEQARIYA
                     ARGQDEEAIRLLQPLVEANPRQPQYKNWLSLLQEKKLQKQLQQADSLLASQQFNAASA
                     MYLQVLQEAPNNRAASDGVQKVQSARQHAAQIEQVKIAIAAEDWAGAEFGLRSVLAEQ
                     PAHAEARQLFEQLEQKKNEKSSVVRPLQSSLKKKVTLELKNTPIKQAFEYIGKAGNLN
                     FSFDQELNEGIRVNVLLRETPIEQALEVILTSHQLGKKVLNSNTLLIYPQARAAQYEE
                     LFVRSFYLTHMDAKRALNMMKTVLRASDVYIDEKLNTLVIRDTLDRIKVAEKLIASQD
                     LNEPEVMLEVEVMEINRRNLENLGIQYPSQIGVGVQGNAAGNGSGTQPQAGKLTLTEL
                     RNFNGNLGVFTINDPALVLNLLHQDTDTNLLASPKIRVKNRDRAKIHVGDKIPVLTSV
                     ASAQGFVSQNVNYIEVGIKLDVEPVIQMQDQVSIKVGMEVSNITDQVTTSSGVLAYTI
                     GSRNANTTLQLRNNETQILAGLFRDDEQKTQRKVPGLANLPLIGRLFSNNNQDKRKNE
                     IVLLITPRIMHNLSPASSVYSLIPSGIAAAANPIGAGGARAVQASEPVSAAPAVTPQS
                     TQSNNVRNDSGFANQMMNSTDTGTVQP"
     gene            complement(3436..3930)
                     /locus_tag="VNZ84_03385"
     CDS             complement(3436..3930)
                     /locus_tag="VNZ84_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66027.1"
                     /translation="MTRRWLVWGALVCTLLACWWVEQSEQTEPPVAMATQAPVVQRRT
                     PLAQAMPALKATDTPAVTAESPPAEAEDAGVDLFAVLEMPAMATAESEVPAAVNPYTY
                     AGRLQEAGRWTVFLTDGQQQYVVHEGDHFAEGWRVAVLDQQKVVLQHAADRFEIRLDD
                     GVVF"
     gene            complement(3927..4487)
                     /locus_tag="VNZ84_03390"
     CDS             complement(3927..4487)
                     /locus_tag="VNZ84_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66028.1"
                     /translation="MEVLTQGWQRLCFTSQQQVLRHRWLTGMLLLAGLLAGLVVQAWW
                     QQQSLQQRLQQLPMPAVIAAKTAKPEAKKPLMADTVLTAWPVEEEAERISAEILTEAD
                     AMGMIFERAEFQSLPVAHASLQVQRIKLPLKGEYMQVRQFLRQVLQRYPSLALSQFKL
                     QRTDVMQPAIEAYVEFSLYTRKRGQP"
BASE COUNT          968 a         1335 c         1153 g         1108 t
ORIGIN      
        1 tccctctcaa ttgtttaatc gctgggcgag gcaaaagcct gatgtcatta acacaacttt
       61 acacaaatgc gaaaatgaga aggaaaaagt cttgaaaacc gatgtgaaag cagcgtgtta
      121 tttacatgct tgcggcattg ctgctgatcg gttgaggcag gtacacaaaa taccattcac
      181 cgtaataacg cttgttattc aagtgttcga ggttgccgcg ataacctttg gatttaaaag
      241 gcggtttgtc tgacaagctg taaatgccca taatgccgcc actgggcgct tctacaatcc
      301 cccatccttc acggtgcgta atagggtctt catagagttt acgcagatgc cgtcgcgctg
      361 tcgggaagcg cttatcttca tacaaggcct ctagcgtggg tggataggtt ttgaccacgc
      421 caggtgtctg gttgtagtaa cggccaatgg cgtcgcggta agccagcccg accttgatca
      481 gctcatgctc acgctcccgt aacacctgcg tttgccagcg catgctggct tgcgccataa
      541 ccagtccact gagcatgatc agcataaaca tgcctatcaa tacaaaaccg ctggagagtg
      601 gcttaccaca gtgcatacgg cgtcccgtcc tcggacagca aagccgaacc gctatgcaca
      661 tcaaacacgc tgccctcttc ctcaatatta ggtggcaagg taatcaccca cgtgtcggca
      721 cgatcagtga tgggatctgg cgggatcgac ttcaggtatt gccgctcgac cagctcttcc
      781 agggtttccg gatagcggtt tttatcgcta taaaacttgt cgatggcatc acgcatggtc
      841 agcaggtcat ggcgcaagct ggtttccttg gcgcgttgca gcatatgcat gaattttggc
      901 gaaacaatcg tgagcagcat ggccaggatc gccagcacca ccagcatttc aatcagggta
      961 aatccgcgtt tcatgcttac cactcactat aaggttgctg gttaatgccc atcgcaggcg
     1021 aacgtgaaga gacgtcatag acatcggtgg ccatgtcatc gagcgcgcca tcataagcgc
     1081 ggatgcgcca ggtctcggca ttggtttggc tactgtccgc ggcaaacgga tcacgcggga
     1141 tacgccgtaa aaaaaacagt tttttctttt tggggtcttg ggcattttcc accccttgca
     1201 ccaggatcgc caaattgggc ggataaccac tgccatctgc ttttttcttg atgagtccct
     1261 ggtcgaccgc gtctttatag gcatcgagtg catcgcggat ttgccacaag gcgcgtttga
     1321 gctcccgctc tttctcacgc ttggcattga gttgcaccac cgggctggcc gaggtgacca
     1381 gcagtgccag taaagccagg gtcaccagca gctccaccag tgtgaatccc tgtgttttgc
     1441 gccgcacccg accagtcacc tgccagctcc gttatggttg tacggtacca gtatcggtac
     1501 tgttcatcat ctggttggca aagccgctgt cattacgcac attattgctt tgcgtggatt
     1561 gcggcgtcac cgcaggcgca gcggacacgg gttcactggc ttgtaccgca cgtgccccac
     1621 cggcacctat tgggttcgcc gccgccgcaa tccccgacgg aatcaggctg tacaccgagc
     1681 tggcagggct caggttatgc atgatacgcg gcgtgatcag cagcacaatt tcgttcttgc
     1741 gcttgtcctg gttattgttg ctgaacagcc tgccgatcaa tggcaagttg gccaaaccgg
     1801 gcactttgcg ctgcgttttc tgctcatcgt cacgaaacag cccggccagg atttgcgttt
     1861 cattattgcg cagttgcaag gtggtattgg cattgcggct gcctatggta taggccaata
     1921 cgccagaact ggtagtgacc tggtcagtga tattgctgac ttccatgccg accttgatac
     1981 tgacctggtc ctgcatctgg atcaccggtt cgacatccag cttgataccg acctcaatgt
     2041 aattcacatt ctggctgaca aagccctgcg cgctcgcgac actggtcaac accggaatct
     2101 tgtcgccaac atgaatcttg gcgcggtcac ggttcttgac gcggattttg gggctggcca
     2161 gcaagttggt atccgtatcc tgatgtagca ggttcaacac cagcgcagga tcattgatgg
     2221 taaacacacc caggttaccg ttaaagttac gcagctcagt cagggtcagt ttaccggcct
     2281 gtggttgggt gccactgcca ttccccgcgg cattgccctg cacaccgacc ccaatctggc
     2341 ttgggtactg aatgcccagg ttttccaggt tgcggcggtt aatctccatc acctccacct
     2401 cgagcatgac ttctggctca ttgaggtctt gtgaggcaat cagcttttcc gccactttaa
     2461 tccggtccag ggtgtcacga atcaccagcg tattcagctt ctcatcaata taaacatcac
     2521 tggcacgcaa cacggttttc atcatattga gcgcacgttt ggcatccata tgcgtcaggt
     2581 aaaaactgcg cacgaacagc tcctcatatt gggcagcgcg ggcttgcggg taaatcagca
     2641 gcgtattact gttgagcacc ttcttgccca gttggtgact ggtcaaaatc acctcgagcg
     2701 cctgctctat cggcgtctca cgcaacaaca cattgacgcg tatgccctca ttgagctcct
     2761 ggtcaaacga aaaattcagg ttgcccgcct tgcctatata ttcaaaggcc tgtttgatcg
     2821 gggtgttttt cagctccagt gtgactttct tcttgagtga cgattgcagg gggcgcacca
     2881 cactgctttt ttcgttcttt ttctgttcca gttgctcaaa caattgccgc gcttcggcat
     2941 gcgcaggttg ttcggccaag acactgcgca agccgaattc cgcaccggcc cagtcctctg
     3001 ccgcaatggc gattttcacc tgttcaattt gcgcagcatg ctggcgggct gactgtacct
     3061 tttgtacgcc atcactggcg gcacggttat tcggcgcctc ctgtaatacc tgcaggtaca
     3121 tggcactggc cgcattgaat tgttgcgaag cgagcaaact gtccgcctgt tgtaactgct
     3181 tttgcagctt tttctcctgc aacaggctga gccagttttt gtattgcggc tgccggggat
     3241 tcgcctctac caaaggctgc aacagccgga tcgcttcctc atcctggcca cgtgccgcat
     3301 aaatcctggc ttgctccagc gtggcatctc cagcaggatt ctcagggcct aaaccggtgg
     3361 ggctgaagct actgcaactg accagccagg gcaatgcaca ggcccatacc caatttttat
     3421 gattcacttt cttgttcaaa acaccacccc gtcatccagc ctgatttcaa agcgatcggc
     3481 tgcgtgttgc agcacgactt tctgttggtc gagcaccgcc acccgccagc cttcggcaaa
     3541 gtggtcgccc tcatgcacga catattgttg ttgtccatcg gtcaaaaaca cggtccagcg
     3601 ccccgcttcc tgcaagcgcc cggcataggt ataaggattc accgcagcgg gaacttccga
     3661 ttctgcggtc gccatcgcag gcatttccag tacagcaaac aaatccacac cggcatcttc
     3721 agcctcggct ggtggcgatt cggctgtcac cgctggcgta tccgtagcct tcagggcagg
     3781 catcgcctgt gccaaagggg tacgccgttg cacgacaggc gcctgggtcg ccattgccac
     3841 aggcggctca gtttgttcgg actgttctac ccaccagcaa gccagcaagg tgcacaccaa
     3901 tgcgccccac actaaccagc gccgcgtcat ggctgacccc gcttacgtgt atataaactg
     3961 aactctacat aggcctctat ggccggctgc atcacatctg tccgctgtaa cttgaactgg
     4021 ctgagtgcca ggctgggata gcgctgcaac acctggcgca agaactgccg cacctgcata
     4081 tactcaccct tgagcggcaa tttaatgcgt tgtacctgca aactggcatg tgcaactggc
     4141 aaagactgaa actccgcccg ctcgaagatc atgcccatgg catccgcctc ggtcaggatt
     4201 tcggcactga tacgctcggc ctcttcttca actggccacg ccgtcaacac tgtatcggcc
     4261 ataagcggct tcttcgcctc aggcttggct gtctttgccg cgatcacggc aggcataggc
     4321 aactgctgca accgctgctg taatgactgc tgttgccacc aggcttgcac caccaaccca
     4381 gccagcaatc ctgccagtag caacatcccg gtcaaccagc ggtgacgcag cacctgctgc
     4441 tggctggtaa agcacaggcg ctgccaccct tgcgttaata cttccatgcc gcctccaaat
     4501 gcaatgtggt catacgcaaa ccattcacct cggtggcttc gctggcattc aacttcacat
     4561 cggc
//
LOCUS       DATMSK010000098         3559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_124869,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000098 DATMSK010000000
VERSION     DATMSK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124869"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..2859)
                     /gene="ppc"
                     /locus_tag="VNZ84_03395"
     CDS             complement(79..2859)
                     /gene="ppc"
                     /locus_tag="VNZ84_03395"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWU66029.1"
                     /translation="MIKKLAASAMLSAEAQLAENIQLLGALLDKAILDVEGEEVAQTI
                     AAIRQAALRFHQTHDQQASLDLEQLLANLSLEHTVRVVRALAYFKHLANLAEDLYGQQ
                     LGHCQQSTPAPGMLSYTLANIKAASIPAQAIKELLQTALISPVLTAHPTEVQRKSVLD
                     IERQIAELLAERAHLVSERQLARNSLLLQGAVSALWQTRMMRYSKLSVQNEIENALTY
                     YEATFLHVIPEILQDIERDLSGLLPEMVLPGFLRMGSWIGGDRDGNPFVNGTTLRDSV
                     RLQATTLFKFYLQELAALKRELAVSTRVVGVSEAVLHMTKTSRDQSQHRLDEPYRLAL
                     NGVYDRLLATANQLLPEGGWVLEPGIVADAYENAEALLVPLETIAASLRQHQGEALIY
                     PRLGKLIKAIHSFGFHLATVDIRQSSDVHEAVITELLHKAGHDFDYASFNEDEKIGVL
                     LEELKQPRLLFSPFQQYSELVHKEIGVLVAVRETRERFGEHAVRQYIISHTETLSDLL
                     EVALLQREAGLLRGVWGSANIQVDLNIVPLFETIADLRDAPMIMGKWLSLLGIRHIIR
                     HQGNEQEIMLGYSDSNKDGGFLTSNWELYKAEISLVELFNQANVRLRLFHGRGGTVGR
                     GGGPTYQAIMAQPKGTVDGQIRLTEQGEIISTRYADPVVGRQHLETLIAATLDATLFP
                     ADQLESSKRRAFESVMEALSTTAMTSYRSLVYETPGFAEYFFNTTPIDEIAGLNLGSR
                     PAARKSTRRIEDLRAIPWGFSWGQCRLLLPGWYGLGSAIQHFLQQDPALKESRLAMLL
                     EMQAHWPLFNTLINNVDMVLAKTDLIVARHYAHLLEDRELREEIFSRIAHEHKLTTDA
                     VNLLLGTTQRLASQPVIAKSIRDRLPYLDPLNHLQVEMIQRYRKGETDEKLKWAIPLT
                     INGIATSLRNTG"
     gene            3159..3374
                     /locus_tag="VNZ84_03400"
     CDS             3159..3374
                     /locus_tag="VNZ84_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66030.1"
                     /translation="MMLINLDHWLQQILVVWQSLSDHVWQQDAFLLLVCLVACLLLFL
                     SGTVLGLFVQSLWGIFMSGRHADEEHK"
BASE COUNT          848 a          968 c          880 g          863 t
ORIGIN      
        1 aacacagaca ctaattgcag tctgtgaata aaaaaaggac aagttctctt gtcctttttt
       61 tatgtccgct tgcattgatt aacctgtatt tcttaagctc gtcgcaatac cattaatggt
      121 caatggaata gcccatttca acttctcgtc cgtttcacct ttacggtaac gctggatcat
      181 ttctacctgc aaatgattca atgggtccag gtaaggcaaa cggtcacgga tcgattttgc
      241 aatcacaggc tggctggcca accgctgagt ggtcccaagt agcaggttga ccgcatctgt
      301 cgtcaattta tgttcatgcg cgatacggct gaaaatctct tcgcgcagct cacgatcttc
      361 cagtaagtgt gcataatgcc gcgccacaat caggtcagtt ttagccagca ccatatccac
      421 attgttgatc aacgtattaa acagcggcca atgtgcctgc atttcgagca gcatggccaa
      481 acgggactct ttgagcgcag gatcttgctg caagaaatgc tggatggcac tgcctaaccc
      541 ataccagcca ggcaatagca ggcggcattg cccccaggag aacccccacg gaatcgcgcg
      601 caagtcttca atgcgccggg tagacttacg cgcagccggg cggctgccca aattgagtcc
      661 agcaatttcg tctatcggtg tggtattaaa aaaatactcg gcgaaccctg gtgtttcata
      721 caccaggctg cggtaactgg tcatggcggt ggtcgacaaa gcctccatca cgctttcgaa
      781 agcgcgccgt ttggaggatt ccagctggtc tgccgggaac aatgtggcat ccaatgtcgc
      841 cgcaatcaat gtttccaagt gctgacggcc taccaccggg tcagcgtagc gtgtagagat
      901 gatttcccct tgctcagtca ggcggatctg gccatcgacc gtgcctttgg gttgggccat
      961 gatggcctgg taagtcggtc caccaccccg gccgacggtg ccaccacggc catgaaacaa
     1021 gcgcaacctg acatttgcct ggttaaacaa ctcaaccagc gaaatttctg ctttatacaa
     1081 ctcccagttt gaggtcagga aaccgccatc cttgttgcta tctgagtaac ccagcatgat
     1141 ttcctgctcg ttcccctgat ggcgaataat atgacggatg ccgagcaggc tcagccattt
     1201 gcccatgatc atgggggcat cgcgcaagtc agcgatggtc tcaaacaagg gcacaatatt
     1261 caaatccacc tgtatattgg ccgatcccca cacaccgcgt aacaagcctg cctcgcgttg
     1321 cagcaaagcc acttccagca ggtcggataa cgtttcggtg tgcgaaataa tatattggcg
     1381 cacagcatgt tcgccaaagc gctcccgcgt ttcgcgtacc gcgaccagca cgccaatttc
     1441 cttatgcacg agctcggaat attgctggaa tggggaaaat agcaggcgcg gctgcttgag
     1501 ctcctcaagc aatacgccta ttttttcatc ttcattaaaa ctggcgtaat caaaatcatg
     1561 accggcctta tgcaacaatt cggtgatcac ggcctcatgc acatccgatg attgacggat
     1621 atccaccgtg gccaggtgaa aaccaaaact atgaatggct ttgatcagct tgcccaggcg
     1681 cggatagatc agcgcctcgc cttgatgttg gcgcaaagaa gcggcaatcg tttccagcgg
     1741 taccagcaag gcttctgcat tttcatatgc atcagccacg atccctggct ccagtaccca
     1801 gcctccctcc ggaagcaatt gattagccgt ggcaagcagc cggtcatata cgccgttcaa
     1861 tgccaggcgg taaggctcat caaggcgatg ttgcgattga tctcgcgagg ttttggtcat
     1921 gtgcaggacg gcctcactga caccgacaac acgtgttgac acagccagtt cgcgtttgag
     1981 tgcagccagc tcctgcaagt aaaacttgaa taaggtcgtg gcctgcaaac gtacactgtc
     2041 acgcagggtt gtaccgttca caaacgggtt gccatcgcgg tcgcccccta tccagctgcc
     2101 catgcgtaaa aaaccgggta ataccatctc aggcaacagg ccggacaggt cgcgctctat
     2161 atcctgcaag atctcaggga tcacgtgcaa aaaagtggct tcataatagg tcaatgcatt
     2221 ctcgatctca ttctgcacac tgagcttgga ataacgcatc atgcgcgttt gccagagcgc
     2281 agagaccgcc ccttgcagca acaaactatt gcgtgccagt tggcgctcag aaaccaggtg
     2341 cgcacgctct gccagcagct cggcaatctg tcgttcgatg tccagcacac ttttgcgttg
     2401 cacttcggtt ggatgcgccg tcagcaccgg ggagattaag gcagtttgca gcagctcttt
     2461 gatggcctgc gccggaattg aggcagcttt aatgtttgcc agagtgtaag aaagcatgcc
     2521 tggcgcgggc gtactctgct ggcaatgacc aagctgctgg ccatataaat cctcagcgag
     2581 attggccagg tgtttaaaat aggccaatgc ccgcaccact cgcaccgtat gctccaggct
     2641 caggttagcc aataactgtt caaggtccag agaggcctgc tgatcatgtg tctggtgaaa
     2701 gcgtagtgcc gcctggcgaa tcgcagcaat tgtctgcgca acctcctcgc cctctacatc
     2761 cagaatcgcc ttgtcgagca aggcgcccaa tagctgaata ttttcggcca gttgcgcctc
     2821 tgctgaaagc atggcgctgg ccgcaagttt ttttatcatg atgcctctct cctctagctc
     2881 agtactactc tagcaacaag caggccatct ttaggtggca tttataccat taaaaatcat
     2941 atggttataa aaaccacatg tgcaatttgc actggcatca ggcaatatcc gcaccgattt
     3001 ggcacggtgc aaacatgctg aagtgcgcat gatgcgttga caccttcagc ggtcaaaact
     3061 cactgaaggt gaatggagag tatgggaagt gataaaggtc tagtgagtca tacttttcgc
     3121 aatgacttat tcagtctttc ctcaggcata atacgcggat gatgctcatc aacctcgatc
     3181 attggctaca gcaaatactc gttgtctggc aatcattgtc agatcatgtg tggcagcagg
     3241 atgctttcct gctgctcgta tgtttggtcg cgtgcctgtt gctatttctg agcggcaccg
     3301 tgctcggctt gtttgtgcaa tcgctatggg gaatttttat gtccggtcgc cacgcggacg
     3361 aagaacataa gtagtcaact tggtttactt catggcagtc gcgctcaact gctgatagag
     3421 cgccgccagt tcaccagcca catctggccc atgatcctca tactggtgac cgccagacct
     3481 ggcataccag tatttgctat tccaggtatc gccttcaatc ttgtgtagca cggcgtgaat
     3541 ccagtttgcc gtgctgtca
//
LOCUS       DATMSK010000099         6066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_383664,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000099 DATMSK010000000
VERSION     DATMSK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383664"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..624)
                     /locus_tag="VNZ84_03405"
     CDS             complement(169..624)
                     /locus_tag="VNZ84_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWU66031.1"
                     /translation="MNIEQLRASKILIVDDAEANLRLLEELLTKEGFEQVISTTDPTK
                     TMDLVNAFQPDLILLDLMMPVLDGFAVLDQLSHHVGAHEYLPVLVLTADATIATRRKA
                     LALGAKDFLTKPFDPMEAMLRVWILLETRWLFTQLKQHQPNLQLPGYAS"
     gene            complement(642..2618)
                     /locus_tag="VNZ84_03410"
     CDS             complement(642..2618)
                     /locus_tag="VNZ84_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU66032.1"
                     /translation="MKMVLDAFNNATRRLDHLWQDLSLTTKGIVLNALPLTVLLASLA
                     LIFESEQQAALLERRVQNALQIQQDIQTLQTLLLEASTGVRDFLLTGNRQFLAGYESA
                     RQSMPQLLSSLEHNLDDRSQQHLLQVIHPLVAKNLDDLAVLASHSEEEEADETLIGKF
                     SSQGNALNQLRAHLDKMSQRESAIVDQDKKEVVYERKRNLRITLMAVIAGVLGSLIGV
                     WLFTQTIVARVRTIRDSAIHLVKGEPLALPSISRDELGELTSELEHASQLLTQSAQEA
                     HLARVEAEEASAAKSNFLSRTSHELRTPLNAILGFAQILENDLPEGRQKDSATLIHNA
                     GQHLLKLINEVLDISRIESGDISMSMEVIVLNHLLEEAYHYLKPLEKVRDITIETHFE
                     PVLSVRADRQRLLQVILNLLANALKYGPENTSVAFSAYRQHGHIRVDVKDQGPGIPAA
                     LRSRLFTPFDRLGAERTVTEGTGLGLVLSKQLMDAMGGQIGVAEDQSLFWISLPEVVQ
                     TIQAPTVNPSVAAEAPLPSTAATTPAAKRHVLYVEDNHSNQALIEAIMSKHRHLKLHL
                     AASMQESMVWLRDMQPDLLLLDLNLPDGSGESLIQHIQTSRADLQHIPVVILSADALP
                     ETMQRLNSLGITHYFTKPLNVAAFNQLLLKLLPE"
     gene            complement(2622..3386)
                     /locus_tag="VNZ84_03415"
     CDS             complement(2622..3386)
                     /locus_tag="VNZ84_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWU66033.1"
                     /translation="MPNDTPPPAESAFALRNALKQNQVQLAHDRLFERLGQKLFMLGW
                     SDDLLQWLLDEICMQFGAAHGLISQQHAGALKILAQRGKTFPVGARVPMLGALGQWLK
                     EPIHFEVHTQFVPSLWTLDAQTSASVTCYHLPIACQQRAVGLFGLITGNPLSQGNLQL
                     LHSVCGLLGFALQGHAQTASVVDDSYMQKLTPREREVFALLPSGASNAALAQKLGISP
                     GTVKIHIERILSKLDLNDRTQAAVKAVEAGFKSDGL"
     gene            3528..3785
                     /locus_tag="VNZ84_03420"
     CDS             3528..3785
                     /locus_tag="VNZ84_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66034.1"
                     /translation="MRKVTAIVLMALGFGLALPAQAAESKAGETVQNTHHVQFLGKRA
                     YQQPLVAKSDAEQAWEGATLRVDQTNQQQVLKMQSLGKRAY"
     gene            3869..4156
                     /locus_tag="VNZ84_03425"
     CDS             3869..4156
                     /locus_tag="VNZ84_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66035.1"
                     /translation="MSKLLLALLVTFGLMANAQADKSADDEATINQMHQHNFLGKRPY
                     AKTPVAKQNPDEKWVGAGIVTDKPEPGFDKHQQMRLNFIGRRPYNAGNGTD"
     gene            4264..5850
                     /locus_tag="VNZ84_03430"
     CDS             4264..5850
                     /locus_tag="VNZ84_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWU66036.1"
                     /translation="MSTSVQGSATTDLFKHINRDLPASIVVFFVALPLCLGIALASGA
                     PLFSGVIAGIIGGIVVGLASGSQLGVSGPAAGLAVIVLAAISNLGSYEAFLAAVVLAG
                     VFQFLLGQFKAGFIAYFVPSSVIKGMLTGIGLLIILKQIPHAFGYDADFEGDEAFEEA
                     GGHNTFSDILQAWDLLTPGAILIALVSFAILILWDTVLTKKHRIFQLLQGPIVVVLVG
                     IAMNWAFVNEWLNFKLSADQIVSLPVASSLGDFAGFFTHPDFSQIAQPLVIKTAIVMA
                     IVASLETLLCVEATDKLDPLKRVTPTNRELKAQGLGNMVSGLIGGLPITQVIVRSSAN
                     ITFGAQTKLSAVLHGFWLLLSALTIASLLTMIPLASLATILIMVGYKLAKPSLFKQMY
                     ELGWEQFVPFIVTILAIVLTDLLTGIGLGLVVAVFFTLHHSYRNSHYIKQTRTSEEGK
                     DVYHLVLAEEVSFFNKASILDVLESIPANSKVVIDCTNTKSIAYDVVEFIHDYQQHAK
                     LKNIMVETIQFHPPKHLLGH"
     gene            5899..>6066
                     /locus_tag="VNZ84_03435"
     CDS             5899..>6066
                     /locus_tag="VNZ84_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882641.1"
                     /note="macrophage inducible 5; Mig-5; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWU66037.1"
                     /translation="MYKHPLLDRDKMTAREALDILIEGNRRFTSDREDDKDFKTLIQI
                     TKDKQHPFASFL"
BASE COUNT         1448 a         1446 c         1598 g         1574 t
ORIGIN      
        1 gtgagcctgc aagccaggct tgggaaagca ccagcttgca aactgacaag caaccagcgg
       61 tacaacaggt ggccgagcaa aaagaagagt ttcgtaaaaa actcaatctg caatatgcga
      121 gcaagcgttc ttacgtgaag cctgatgccg ccgagtaggc gtgcaggatt acgatgcata
      181 tcctggtaat tgcaggttag gctggtgttg tttgagttga gtaaataacc agcgagtttc
      241 cagcagtatc cagacacgca gcatggcttc catcgggtca aaaggcttgg ttaaaaagtc
      301 ttttgcaccc aaggccagcg ctttgcgccg cgttgcaatg gtggcgtctg ctgtgagcac
      361 cagcaccggc aggtattcat gggcacccac atggtgggag agctgatcca gtacggcaaa
      421 accatccagt accggcatca ttaaatccag caaaatcagg tctggctgaa acgcattgac
      481 cagatccatg gttttggtcg ggtccgtggt gctgatcacc tgttcaaagc cttctttggt
      541 cagcagttcc tccagtaagc gtaaattcgc ctcggcatcg tcaacgatta aaatcttgga
      601 agcgcggagt tgttcgatat tcatatggct agctcttggt tttattcggg caatagtttt
      661 aatagtaact ggttgaacgc tgcgacattc aatggcttgg taaagtagtg tgtgatacca
      721 aggctgttga gccgttgcat ggtttcgggt aaggcatctg cgcttaaaat cactaccgga
      781 atatgttgca aatccgcacg gctggtttga atgtgctgga taagcgactc gcccgagcca
      841 tctggcaggt tgaggtccag cagcagcaag tctggctgca tgtcacgtag ccagaccatg
      901 ctttcctgca tgctggcggc caggtgcagt ttgaggtgcc ggtgtttgct catgatggcc
      961 tctatcagcg cctggttgct atgattatct tcgacataca gcacatgacg ttttgcggct
     1021 ggcgttgtcg ctgctgtgct gggcagcggg gcttcagcgg ccactgacgg gttgacggtg
     1081 ggtgcctgga tcgtttggac tacctctggc aagctgatcc agaacaggct ctggtcttca
     1141 gcgacgccaa tctgtccacc catggcatcc attaattgtt tgctcagcac caggcctaag
     1201 cctgtgcctt cagtcactgt gcgttcagcg cccagccggt caaacggggt aaataagcgt
     1261 gagcgtaagg ctgccgggat gcctggtccc tgatctttca catcgacacg gatgtggccg
     1321 tgctggcggt aagcactgaa ggccacactg gtattttccg gaccatattt caaggcattc
     1381 gccagcaggt ttaagatgac ttgtaacagc ctttgcctgt cggcacgcac gcttaatacc
     1441 ggttcaaagt gcgtttctat ggtgatgtcg cgcaccttct ccagcggttt taagtaatgg
     1501 taagcttctt ccagcaagtg attcaggaca atcacttcca tggacatgct gatgtcgcca
     1561 ctttcaatgc gcgaaatatc cagcacttca ttaatgagtt tcaacaagtg ctgacccgca
     1621 ttgtgtatga gggtggcact gtctttttgc cgtccttcag gcaaatcgtt ctcaagaatt
     1681 tgtgcaaagc cgaggatggc attgagtggc gtacgcaact catgactggt acgtgataga
     1741 aagttacttt tggcggcact ggcttcttct gcctcaacgc gggccagatg tgcttcctgg
     1801 gcactctgcg tcaacagttg tgaggcatgt tccagttcgc tggtcagttc gcccagttca
     1861 tcgcggctga tactgggtaa cgctaaaggt tcacctttga ccaggtgaat agcactgtcg
     1921 cggatagtcc gcacacgagc gacgatggtt tgtgtaaata gccacacgcc tatcagagaa
     1981 cccaggaccc cggcaatcac ggccatcagc gtaatacgta agttacgttt gcgctcatac
     2041 accacttctt ttttatcctg gtcgacaatc gccgattcgc gttgactcat tttgtcgaga
     2101 tgcgcgcgta attggtttaa agcattgcct tgcgagctaa acttgccaat taaggtctca
     2161 tccgcttctt cttcttcact atgactggcg agcactgcca gatcgtccag gtttttagct
     2221 acaagaggat gaatgacctg caacaggtgc tgttgtgagc ggtcgtccag gttgtgttcc
     2281 agcgaactga gcagctgggg catgctttgc ctcgcgcttt cgtagcctgc caggaactgg
     2341 cggttacctg tcagcaggaa gtcacgcacg cctgttgagg cttctagcaa cagggtttgc
     2401 aaggtctgga tatcctgctg gatttgcagc gcattttgca cacgcctttc cagcagggca
     2461 gcctgttgtt cgctctcgaa aatcagcgcc agcgaggcca gtaatactgt gaggggcaag
     2521 gcattcagca caatgccttt ggtggtcagc gataaatcct gccatagatg gtccaggcgg
     2581 cgggtcgcat tattgaatgc atcaagtacc attttcatgc cttacagccc gtcactcttg
     2641 aatcccgcct ctacggcctt gaccgctgcc tgggtacggt cattcaggtc cagcttgctt
     2701 aaaatgcgtt caatatggat ttttaccgtg ccgggtgaaa tccccagctt ttgtgccagg
     2761 gcagcattgc tggctcctga cggcagcaag gcaaacactt cgcgctcgcg tggggtgagt
     2821 ttttgcatat aactatcatc gaccacgctg gcggtttggg catgtccttg cagtgcaaat
     2881 cccagcaggc cacaaacact gtgcaataac tgcaaattac cctgggataa gggattgccc
     2941 gtgatcagcc cgaataagcc tacagcacgt tgctggcacg caatcggcag gtgatagcaa
     3001 gtcacggatg cgctggtttg ggcatctagc gtccacaggc taggcacaaa ctgcgtgtgg
     3061 acttcaaagt gtatgggttc ttttagccat tgccccaggg cgcctaacat gggcaccctt
     3121 gcaccgaccg ggaaggtttt gccgcgttgc gccaggattt tcaatgcacc ggcatgttgc
     3181 tggctaatca ggccatgtgc tgcaccgaac tgcatgcata tttcatccag caaccattgc
     3241 aacagatcat cggaccaacc gagcatgaac aatttttgcc caaggcgttc aaacagtctg
     3301 tcatgagcaa gctgtacctg gttttgcttg agtgcattac gtaaggcaaa tgcactctct
     3361 gcaggagggg gtgtgtcgtt tggcatatgc cgtttagtat ataccgaact acctatatcg
     3421 ggagattttt tgttgttaga ctgcgggcaa aatgcaaacc atcgtgaatg catcaagatt
     3481 cacctcacca agtaaacagt ttaatgaatg atttaaggag aaagaaaatg cgtaaagtaa
     3541 ctgcgatcgt tttaatggcc ctgggttttg gcttggcatt gccagcgcaa gcggctgaat
     3601 ctaaagcagg tgaaactgtt cagaatacac atcatgtgca atttttgggc aagcgtgctt
     3661 accagcaacc gctggtagcc aaatctgatg ccgagcaggc ttgggagggt gccacattga
     3721 gagtggacca gaccaaccag caacaagtgt tgaaaatgca atcactgggc aagcgagctt
     3781 actagtcagt gttgcgtaag gttgttgcga cataataaga gtacatctgc acaaggtgta
     3841 taacaaacaa attatgtagg agcgaaaaat gagtaaatta ttattggcac tattggtgac
     3901 atttggttta atggctaatg cacaggcaga caagtctgca gatgacgagg ccaccattaa
     3961 ccagatgcat caacacaact ttttaggtaa acggccttac gccaaaacac cggtagccaa
     4021 gcagaaccca gatgaaaaat gggtgggcgc gggcatcgtg acggacaagc cggagccagg
     4081 ctttgataag catcagcaaa tgcgtttgaa ctttataggc agacgtccct ataacgcagg
     4141 gaacggcaca gattaactat aaacgctcta ctgctctgaa caataacccc gcatgattgc
     4201 ggggttttgt gtgtcataaa gcaatataca taaaaataat tttgtttgtg aaggataaac
     4261 accatgtcaa cctctgtgca agggtctgcg acaacagacc tgtttaagca catcaatcgc
     4321 gacttgccag cctcgattgt tgtgttcttt gtggctttgc ccctttgttt ggggattgcg
     4381 ctggcttccg gtgcgccgct attttccggt gtgattgccg ggattattgg cggtatcgta
     4441 gttgggctgg ccagtggctc gcaattgggg gtcagtggcc cggcggccgg tttggctgtc
     4501 attgtgctgg cggccatcag taaccttggt tcttacgaag ctttcctcgc agctgtagtg
     4561 ctggccgggg tcttccagtt tctgctaggt cagttcaagg ctggttttat tgcctatttt
     4621 gtgccgtcct ctgtgattaa aggcatgctg acagggattg gcctgctgat tatcctgaaa
     4681 cagattccac atgcatttgg ctatgatgcc gactttgagg gcgacgaggc atttgaagaa
     4741 gcaggtgggc acaacacctt tagcgatatc ttgcaggcct gggatctatt gacgccaggg
     4801 gcgatcctca tcgcgcttgt gtcttttgcc atcttgattt tatgggacac cgtcctgact
     4861 aaaaagcacc ggatattcca gttattgcag ggcccgattg tcgtggtatt ggtgggtatt
     4921 gccatgaatt gggcgtttgt aaatgaatgg ctgaatttca agctgagcgc agaccagatc
     4981 gtgagtttgc cagtggctag cagcctcggt gattttgccg gtttctttac gcatcctgat
     5041 tttagccaaa tcgcacaacc cctggtgatc aagaccgcga ttgtgatggc aattgtcgcc
     5101 agcctggaaa cattattatg tgtggaggca accgataaac tggatccttt aaaacgtgtg
     5161 acaccaacca accgggagct caaggcgcaa gggctaggga atatggtttc tggcctgatt
     5221 ggtggtttac cgatcacgca agtgattgtg cgcagtagcg ccaacatcac ctttggcgca
     5281 caaaccaagt tgtcagcggt attacatggt ttttggctgt tgttgagtgc actcaccatt
     5341 gccagtttgc tcactatgat tccattagcc agcctcgcca ccatcttgat tatggtgggt
     5401 tacaaattgg ccaaacccag cctgttcaag caaatgtatg aattgggatg ggagcagttt
     5461 gtgccgttca ttgtgacgat cttagcgatt gtcttgaccg atttgctcac aggtatcggt
     5521 ttgggtctgg tggtggcggt gttctttacc ttgcaccaca gctaccgcaa ttctcactac
     5581 atcaagcaaa cgcgtaccag cgaagaaggc aaagacgtct accacctggt actggcggaa
     5641 gaagtttcct tcttcaacaa ggccagcatt ctggatgtgc tggaaagcat tccggccaat
     5701 agcaaagtgg tgattgattg caccaacaca aagtcgattg cctatgatgt tgtggagttt
     5761 attcatgatt accagcaaca tgccaagttg aaaaacatta tggtggaaac gatacaattt
     5821 catccaccca aacatttatt ggggcattag tcccaaaaga agcagtgaca ataactaatt
     5881 gatacgagga tacgtaatat gtataagcac ccattactgg accgtgacaa aatgaccgcg
     5941 cgtgaggcac tggatattct gattgagggt aaccgccgtt ttacgagtga ccgtgaagac
     6001 gataaagatt tcaaaacctt gatccagatt accaaggaca agcagcatcc attcgcttct
     6061 ttcctg
//
LOCUS       DATMSK010000100        12126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_77914,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000100 DATMSK010000000
VERSION     DATMSK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 12126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12126
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77914"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VNZ84_03440"
     CDS             <1..355
                     /locus_tag="VNZ84_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWU66038.1"
                     /translation="QEQGRYWQISVNGISIQGSFVAQITQQKNTLHFSGNLLIGNISE
                     TLDQLQTLRLESPLSLDFSKVDEVDTVAVSLILQIQRQLNHQHAQASPVSVIGVPDNL
                     RSLMQLYGVDGFLLS"
     gene            430..1344
                     /locus_tag="VNZ84_03445"
     CDS             430..1344
                     /locus_tag="VNZ84_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU66039.1"
                     /translation="MAAAIEITGITKRFGQFEALRGIDLRIEQGEFFALLGPNGAGKS
                     TLINLLAGLLQPTSGNIRIMGHDVQADYQRARNALGVVPQELVFDPFFNVREMLRFQA
                     GYFGHGPENDVWVDEVIEGLGLTDKAHTNMRQLSGGMKRRALIAQALAHKPPVIVLDE
                     PTAGVDVELRQMLWAFIQRLNQQGHTIILTTHYLEEAETLCERVAMMKQGKIVALDTT
                     RALLKSHAAKQLSLRLDGTLPVTLQPLLKQQSGDEVILALSELSQVETVLSTLREAKV
                     VVQDMQLQEADLEDVFLNLVGNQKGEQA"
     gene            1341..2147
                     /locus_tag="VNZ84_03450"
     CDS             1341..2147
                     /locus_tag="VNZ84_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU66040.1"
                     /translation="MKWLNPFFWLDDKISPQRRGMYTLFKKELLRFWRVAFQTIASPI
                     LTALLYLVIFSHVLESHVKVYDQVSYTAFLVPGLMMMSLLQNAFANSSSSLIQSKVMG
                     NIVFLLLTPLTTLEFFIAFLAASMIRGLLVGLSIYLVALCFVTVPTVHPWWIIAFALC
                     GSALLGTFGVIAGIWADKFDQMAAFQNFVIMPLTFLSGVFYSIHSLPPFWQAVSQLNP
                     FFYMIDGFRYGFFGQGDVSPWLSLAVVGSFLLVLAWLCLKMLKSGYKLRS"
     gene            2210..2455
                     /locus_tag="VNZ84_03455"
     CDS             2210..2455
                     /locus_tag="VNZ84_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HWU66041.1"
                     /translation="MLTAAQLEAYIRSGLECDYLKVNGDDGTHFDAVIVSAAFEGKRM
                     VQQHQLVYAALGDRMRAEIHALSMQTYTPTQWQQKQA"
     gene            2484..2792
                     /gene="grxD"
                     /locus_tag="VNZ84_03460"
     CDS             2484..2792
                     /gene="grxD"
                     /locus_tag="VNZ84_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HWU66042.1"
                     /translation="MDIQAQIKETVTNNKVVLYMKGSPKFPQCGFSSVASQILNACGA
                     EFVAIDVLQDMDVREGIKQYANWPTIPQLYVGGEFIGGADIMRSMYQSGELQELLAKA
                     "
     gene            2795..4060
                     /gene="murA"
                     /locus_tag="VNZ84_03465"
     CDS             2795..4060
                     /gene="murA"
                     /locus_tag="VNZ84_03465"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWU66043.1"
                     /translation="MDKLIIEGGVPLHGEVVVSGAKNAALPILCAGLLAETPLTLTGV
                     PELRDVASTLKLLDTMGVKVSKDGDQVTLDASDVASFEATYEMVKTMRASILVLGPLL
                     ARFGTARVSLPGGCAIGSRPVDLHIKGLQAMGAAMHITHGYIQASTLHLPNRRLQGAK
                     YYMDLVTVTGTENLMMAAALAQGITVLENAAKEPEVVDLAECLNKMGAKITGAGTDTI
                     TIEGVERLTGAEHHIVCDRIEAGTYMVAAAMTGGEVTLKNARADLLEAVIEKLREAGA
                     SISHDSHSITVKSDGKLKAVNVRTAPHPAFPTDMQAQFMAMNTVAEGVSTVIETIFEN
                     RFMHVQELQRMGANIDVQGNTALVQGVSALEGASVMATDLRASAGLVLAGLVADGETV
                     IDRIYHLDRGYEKLEEKLNQLGAKVRRSN"
     gene            4063..4704
                     /gene="hisG"
                     /locus_tag="VNZ84_03470"
     CDS             4063..4704
                     /gene="hisG"
                     /locus_tag="VNZ84_03470"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWU66044.1"
                     /translation="MITIALSKGRIFEETTPLLAAAGIQSQEDPESSRKLILPTNRDD
                     VRLVIVRATDVPTYVQYGAADIGIAGKDVLDEHGGEGLYQPLDLNIARCKMMVAVRDD
                     FDYEASIRQGARLKVVTKYVKTAREHFAAKGMHVDLIKLYGSMELGPLVGLADAIVDL
                     VSTGGTLRANKLKAVEEIGAISSRLVVNQASYKVNRPLLQPIIDSFAGAIRKD"
     gene            4737..6032
                     /gene="hisD"
                     /locus_tag="VNZ84_03475"
     CDS             4737..6032
                     /gene="hisD"
                     /locus_tag="VNZ84_03475"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWU66045.1"
                     /translation="MKIKRLNTQDAGFDAELKALLAFETAQDDNIDQVVANILKDVKA
                     RGDAAVLEYTNRFDKTNASSLAALEIPHAELNAALNSLPAEQREALRAAAERVRNYHE
                     KQVMQSWQYTEADGTLLGQQVTALDRVGLYVPGGKAAYPSSVLMNAIPAKVAGVQELV
                     MVVPTPNGEKNSLVLAAAAVCGVDRVFCIGGAQAVGALAYGTETVPQVDKIVGPGNAY
                     VAAAKRRVFGVVGIDMVAGPSEILVISDGKSNPDWTAMDLFSQAEHDELAQAILLSPS
                     AEFLDQVQASIERQVSEMPRQEIIRTSLQNRGALIQVKDLAQAAEICNYIAPEHLELS
                     MDDALAFSKQIKHAGAIFMGRDTCEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQKR
                     SSLIMVSSEGAQTLGKIAATLAYGEGLQAHARSAEYRLK"
     gene            6043..7101
                     /gene="hisC"
                     /locus_tag="VNZ84_03480"
     CDS             6043..7101
                     /gene="hisC"
                     /locus_tag="VNZ84_03480"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWU66046.1"
                     /translation="MSQYWSDIVHRLTPYVPGEQPKLQNLVKLNTNENPYGPSPKVIA
                     ALQAEAADSLRLYPDPDSSRLKVAIAEYHGLQPNQVFVGNGSDEVLAHVFQALLKHEL
                     PLLFPDITYSFYPVYCGLYGIDFETVPLNSKFEIAVDDYLRPNGGIIFPNPNAPTGIP
                     VALSEIEGLLKANTNSVVVIDEAYVDFGTESAVALINKYPNLLVTHTYSKSRSLAGLR
                     VGYAVGNAGLIEALIRIKDSFNSYPIDRFAEAGAVAALHDEDYFQQTRQQVIASREQL
                     VADMQALGFEVLPSGANFIFARHPEYSGEKLASGLRDQAVIVRHFKKPARIAEFLRIT
                     IGNESQNQALMQALRSLM"
     gene            7325..8203
                     /gene="hxlA"
                     /locus_tag="VNZ84_03485"
     CDS             7325..8203
                     /gene="hxlA"
                     /locus_tag="VNZ84_03485"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019647 - formaldehyde assimilation via
                     ribulose monophosphate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HWU66047.1"
                     /translation="MAQTQMALDSLDFDATIALAAKVAPHVDILEIGTPCIKHNGIEL
                     LKALRSKFPNNKILVDLKTMDAGFYEAEPFYKAGADICTVLGTADIGTIKGVIDAANK
                     YGKEAQIDLINVKDKKARTLEVVKLGAHIIGVHTGLDQQAAGQTPFADLGLVSGLKTG
                     AKVSVAGGVKAATTKQVVDAGADIVVAGAAIYGAADPAAAANEITKIAHGSGAATKGG
                     NKLLPWIIAAVAAVLVFSLLGKKSEEAAPAAEAPAAEEAAPAEAAPAAEAPAAEEAAP
                     AEAAPAEAAPATEEAK"
     gene            8361..8951
                     /gene="hisB"
                     /locus_tag="VNZ84_03490"
     CDS             8361..8951
                     /gene="hisB"
                     /locus_tag="VNZ84_03490"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWU66048.1"
                     /translation="MSRQAEVTRNTLETQITVSINLDGTGQSELHSGLGFLDHMLDQI
                     ARHGMIDLKVQAKGDLHIDGHHTVEDIGITLGQAFSKALGDKKGLTRYGHSYVPLDEA
                     LSRVALDLSGRPGLAFDCTFTRAMIGEFDVDLIHEFFQGFVNHALVTLHIDNLKGHNA
                     HHQAETIFKAFGRALRMAATPDPRMAGLMPSTKGAL"
     gene            8968..9612
                     /gene="hisH"
                     /locus_tag="VNZ84_03495"
     CDS             8968..9612
                     /gene="hisH"
                     /locus_tag="VNZ84_03495"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWU66049.1"
                     /translation="MSRLQVAIVDYGMGNLRSVANAFKAVAPTVSIAVTSDPALIAQA
                     ERVVFPGQGAMPDCIRELDARDLRQAVLDATQQKPFLGICIGLQMLFEHSEEGDVPGL
                     GVYPGEVKRFPHERMIDEHGAKLKVPHMGWSQVHQTQAHAMWAGIPDQARFYYVHSYY
                     VAPDETSLTAATTAYPFDFTGAIARDNVFAVQFHPEKSATAGLQLLRNFVNWKP"
     gene            9667..10410
                     /gene="hisA"
                     /locus_tag="VNZ84_03500"
     CDS             9667..10410
                     /gene="hisA"
                     /locus_tag="VNZ84_03500"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HWU66050.1"
                     /translation="MLIIPAIDLKDGHCVRLKQGLMEESTVFSEDPAAMARHWVDKGG
                     KRLHLVDLNGAFAGKPVNEDAIKSIVAAVGGDIPIQLGGGIRDLDTIERYLDDGISYV
                     IIGTAAVKNPGFLHEACYAFPGQIMVGLDAKDGKVAVDGWSKLTGHDVIDLAKKFEDY
                     GVESIVYTDIGRDGMLTGVNIEATVALAQALTIPVIASGGVTNLDDVRNLCAVQDEGI
                     MGTITGRAIYEGTLDFAAAQQLADQLSGQ"
     gene            10410..11174
                     /gene="hisF"
                     /locus_tag="VNZ84_03505"
     CDS             10410..11174
                     /gene="hisF"
                     /locus_tag="VNZ84_03505"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWU66051.1"
                     /translation="MGCAKRIIPCLDVTDGRVVKGVNFLELRDAGDPVEIAQRYNDQG
                     ADELTFLDITASSDNRDLILHIIERVAEQIFIPLTVGGGVRKVEDVRRLLNAGADKVS
                     INTTAVLNPQVVEDAAGRFGSQCIVVAIDAKKVDNQPFEWEVFTHGGRNPTGLDAVKW
                     AQKMVSLGAGELLVTSMDRDGTKIGFNNPLNKAISDAVDVPLIASGGVGNLQHLVDGV
                     TVGGADAVLAASIFHYGEFTVQQAKDYMREHGLEVR"
     gene            11171..11554
                     /gene="hisI"
                     /locus_tag="VNZ84_03510"
     CDS             11171..11554
                     /gene="hisI"
                     /locus_tag="VNZ84_03510"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HWU66052.1"
                     /translation="MSWLDQVTWTEDGLVPVIAQEKDTGKILMFAWMNREALQLTRDT
                     GHAVYFSRSRNKLWHKGEESGHTQIVHDIRLDCDNDVVMMTVEQLGGIACHTGRHNCF
                     FQQLQGDQWVTVEPVLKDPKAIYHE"
     gene            11547..11882
                     /locus_tag="VNZ84_03515"
     CDS             11547..11882
                     /locus_tag="VNZ84_03515"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HWU66053.1"
                     /translation="MSDGLNGNDVLSRLSELLEQRKAADPSSSYVAKLYAKGMDSILK
                     KIGEEATETVIAAKGGKAEEIVYETADLWFHTLVMLSHAGLSAQDVLNELARREGLSG
                     IAEKASRIE"
     gene            11879..>12126
                     /locus_tag="VNZ84_03520"
     CDS             11879..>12126
                     /locus_tag="VNZ84_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWU66054.1"
                     /translation="MSADCVFCKIVAGDIPSKTLYEDDDVVVFHDIRPLAKVHFLIVP
                     KTHIETLKDCMPEHQPLLGKMMLLAAKLAAEQGLVGYKT"
BASE COUNT         2897 a         2613 c         3401 g         3215 t
ORIGIN      
        1 acaagaacaa ggccgctact ggcaaataag cgttaatggc atcagcattc aggggagttt
       61 tgtggctcaa attacgcaac aaaagaatac attgcatttc agtggtaatt tactgatcgg
      121 gaatatcagt gaaaccttgg atcaactgca aacattgcgt cttgagtcac cattgtccct
      181 ggacttttct aaagtggatg aggtggacac ggtcgctgtc agcctgattt tgcaaatcca
      241 gcggcagttg aaccatcagc atgcacaggc tagtcccgta tctgtgattg gggtgcctga
      301 caatctgcga agcctgatgc agctctatgg tgttgacgga tttctgttga gttagttgaa
      361 aattaagtct aacagcccca gctcagacga ggtggggttt cgtttttttg ggatagcgaa
      421 aggtcatacg tggctgcagc catagagatt acaggcatta ccaagcgctt tgggcagttc
      481 gaagcgctca gggggattga tttgcgaatt gagcaagggg agttctttgc cttgctcggg
      541 cctaatgggg cgggtaaatc caccttgatt aacttgctgg cgggcttgtt gcagccgacc
      601 agtggcaata tccgtatcat ggggcacgat gtgcaggctg attatcaacg tgcgcgcaac
      661 gcccttggtg tggtaccgca agaactggtg tttgacccgt tttttaatgt acgggaaatg
      721 ctgcgtttcc aggcgggtta ttttggtcat gggcctgaaa atgatgtttg ggtggatgaa
      781 gtgattgaag gcctgggtct caccgataaa gcgcatacca atatgcgtca actctcgggt
      841 ggcatgaagc gacgtgcctt gattgcgcag gcgctggcac acaagccgcc cgtgattgtg
      901 ctggatgaac caacggccgg cgtggatgtg gagttgcggc agatgctgtg ggcttttatc
      961 cagagactca atcagcaagg tcataccatt atcctgacta cgcattacct ggaagaggcg
     1021 gaaacgcttt gtgagcgtgt cgccatgatg aagcagggca aaatcgttgc gctggatacc
     1081 acgcgcgcat tactgaaaag ccatgcggct aaacagttat cgctgcgatt ggatggcacg
     1141 ctccccgtga ccttgcaacc cttgctgaaa cagcagagtg gtgatgaggt gatattggct
     1201 ttatctgagc tgtcacaagt tgaaacggtc ttgagcacgc tgcgcgaggc aaaggtggtg
     1261 gtgcaggata tgcagttgca agaagccgat ctggaagatg tgtttttaaa tctggtgggg
     1321 aatcagaaag gggagcaagc atgaagtggt taaacccttt cttctggctg gatgacaaaa
     1381 tcagcccgca gcgtcgcggc atgtacacgc tgtttaaaaa ggagttgctg cgcttctggc
     1441 gggttgcctt ccagacgatt gcttctccta tcttaacggc cttactttac ctagtgattt
     1501 tttcgcatgt gctggagtcg catgtcaaag tctacgacca ggtcagctat accgcttttc
     1561 tggtgcctgg cctcatgatg atgtctttgc tgcaaaatgc ctttgcgaat agttcgtcca
     1621 gcctgattca gtccaaggtg atgggcaata ttgtgttctt gctgctgacg ccattgacga
     1681 cactggaatt ttttatcgcg ttcttagctg cttcaatgat acgtggcctg ctggtggggt
     1741 taagcattta tctggtcgct ttgtgttttg tgaccgtgcc gacggtgcat ccctggtgga
     1801 ttatcgcctt tgccttgtgt gggagtgccc tgttggggac ttttggtgtg attgccggta
     1861 tctgggctga caaatttgac cagatggcag ccttccagaa cttcgttatt atgccgctga
     1921 cattcttgtc tggcgtgttt tactcgattc attccttgcc gccgttctgg caagcggtgt
     1981 cacagttaaa cccgtttttc tacatgatag atggcttccg gtatggcttt tttggccagg
     2041 gggatgtgtc gccgtggctc agtttggcgg tagtagggag cttcttgctg gtattagcgt
     2101 ggctatgcct aaagatgtta aaatccggtt ataaattacg cagttagcca acattgcagg
     2161 atgcgttttc tgcaatgttg gactgtattt ttcttgaata ggtggttatg tgttaacagc
     2221 ggcgcaactt gaggcctata tccgtagtgg cctggaatgt gattacctga aagtgaatgg
     2281 agatgacggg acgcattttg atgcggtcat cgtcagcgcg gctttcgagg gaaagcgtat
     2341 ggtgcaacag catcaattgg tctatgcagc attgggcgac cggatgcgtg ctgagattca
     2401 cgcgctgtct atgcaaacct ataccccgac gcagtggcag cagaagcaag cctaagcgtc
     2461 gttgaatatt tggagtaatg aaaatggata tacaagcaca aatcaaagag accgtgacca
     2521 acaataaagt ggtcttatac atgaagggta gccctaaatt tccccaatgt ggtttttcca
     2581 gcgtggcttc acagatcctc aatgcctgtg gtgccgagtt tgtagcgatt gatgtactgc
     2641 aggatatgga tgttcgtgaa ggtatcaagc aatatgcaaa ttggccaacg attccacagc
     2701 tgtatgttgg cggtgagttt attggtggtg ctgatattat gcgcagcatg tatcaaagcg
     2761 gtgagttgca agaactgttg gcgaaagcat aattttggac aaattgatta ttgaaggtgg
     2821 cgtgcccttg cacggcgaag tggtcgtgtc tggtgccaag aatgctgcct tgccaatttt
     2881 gtgcgcaggc ttgttggcag aaacaccgtt gacactaact ggcgtgcccg aattgcgcga
     2941 tgtcgcttcc acacttaaat tgctggacac catgggcgtc aaggtcagca aggatggcga
     3001 ccaagtcact ctggatgcca gtgatgtcgc ttcgtttgaa gcgacctatg agatggtgaa
     3061 aaccatgcgg gcttccatct tggtgctggg accattactg gcccgttttg ggactgcgcg
     3121 tgtttccttg cctggcggtt gtgcgattgg ttcgcgcccg gttgatttgc atatcaaagg
     3181 cttgcaggca atgggtgcgg cgatgcacat cacgcacggt tatattcagg ccagtacctt
     3241 gcacttgcct aaccggcggc tacaaggtgc caaatattac atggatttgg taacagtgac
     3301 cggcactgaa aatcttatga tggctgctgc gctggcgcag ggcatcactg tgctggagaa
     3361 tgcggccaaa gagccggaag tggttgacct tgcagaatgc ctgaataaaa tgggcgccaa
     3421 gatcacaggc gccggcacgg ataccattac gattgaaggc gtggaacgcc tgactggtgc
     3481 agagcatcat atcgtctgtg accggattga agcaggaacc tatatggttg ctgccgctat
     3541 gaccggtggc gaggtgactt tgaaaaacgc gcgcgctgat ttgctggagg cggtgattga
     3601 aaagttgcgt gaggcaggtg ccagtattag tcacgacagt catagcatta ccgtgaaaag
     3661 tgatggcaag ttgaaagcgg taaatgtgcg cactgcaccg cacccggcat ttcctaccga
     3721 catgcaggca caatttatgg ccatgaacac agtggcagag ggtgtgtcta ctgtcattga
     3781 gactattttt gaaaaccgct ttatgcatgt gcaagaattg cagcgcatgg gggcgaatat
     3841 tgatgttcag ggcaatactg ccttggtgca gggtgtctct gcgttggaag gtgccagtgt
     3901 gatggcgact gatttgcgcg catctgctgg cttggtgctg gctggtttgg tggcagatgg
     3961 tgagacagtg atcgaccgca tttatcatct ggatcgtggt tatgaaaaac tggaagagaa
     4021 gttgaatcaa ttgggcgcca aagtgcgtag atcgaattaa tcatgattac tatcgcactg
     4081 agtaaaggac gcatttttga ggaaaccacg ccattgttgg cagcggcagg catacagtca
     4141 caggaagatc cggaatcttc acgtaaactg attttgccca ccaaccgtga cgatgtgcgc
     4201 ctggtaattg tacgcgcgac cgatgtgccg acctatgtgc aatatggtgc agccgacatt
     4261 ggcatcgccg gtaaggatgt actggatgag catggtggcg aaggtttgta ccagccattg
     4321 gacttgaata tcgcccgctg caaaatgatg gtggcggtgc gggatgattt tgattatgag
     4381 gccagtatcc gccaaggtgc acgcttgaag gtggtcacca aatatgtgaa aacggcgcgc
     4441 gagcattttg ccgccaaagg catgcatgtg gacctgatta aattatatgg ttctatggag
     4501 ttaggtccat tggttggctt ggcggatgcg attgttgacc tggtgagtac agggggcaca
     4561 ttgcgcgcca acaagcttaa agcggttgag gagattggtg ccatcagctc acgcttggtg
     4621 gtgaaccagg cttcttacaa agtgaaccgc cctttgctgc aaccaattat tgatagtttt
     4681 gctggcgcta ttcgcaaaga ctagctgcat tgataaagat tacttgatag agatctatga
     4741 agattaaacg tttaaatacg caggatgctg gttttgatgc cgaattaaaa gcgttgttag
     4801 cttttgaaac ggcacaggac gacaatatcg accaggtggt cgccaatatt ctcaaggatg
     4861 tgaaagcgcg tggcgacgca gcggtgctcg aatataccaa ccgtttcgat aaaaccaatg
     4921 cttccagttt ggcggcgttg gaaattcctc atgctgaact caatgcagct ttgaatagcc
     4981 tgcctgcaga gcaacgtgaa gccctgcggg ctgcggccga gcgtgtacgg aattatcacg
     5041 aaaagcaagt catgcaatcg tggcagtaca ctgaagcgga tggtactttg ctgggtcagc
     5101 aggtgacggc gctagaccgt gtcggtttgt atgtgccggg cggcaaggca gcgtatccat
     5161 cttcagtgct catgaatgcg attcctgcca aggtggctgg tgtgcaggag ctggtgatgg
     5221 tggtgcccac accaaatggt gagaaaaact cattagtgct ggctgctgcg gctgtatgcg
     5281 gtgttgatcg cgtattctgt atcggtggcg cacaagccgt gggcgccttg gcttacggca
     5341 ctgaaaccgt gccgcaggtt gataaaattg ttggcccagg caatgcttat gtggctgctg
     5401 ccaaacgtcg tgtgtttggc gttgttggca ttgatatggt cgctggccca tcagaaatcc
     5461 tggtcatcag tgatggcaag agcaatccgg actggacggc gatggacttg tttagccagg
     5521 ctgagcacga tgagttggcg caggcgattt tattgtcgcc cagtgccgag tttctcgacc
     5581 aggtgcaggc aagtattgaa cgccaggtca gtgagatgcc acgtcaggaa attatccgta
     5641 cctcactgca aaaccgcggt gcattgatac aggtaaaaga cttggctcaa gcagcggaga
     5701 tttgtaacta tatcgcgcca gagcacttgg aattgtccat ggatgatgcg ttggcgttta
     5761 gcaagcagat caagcatgct ggcgcgattt tcatgggtcg tgatacctgt gaggcattgg
     5821 gtgactactg tgctggccca aatcacgtat tgccaacctc acgtactgcg cgtttcagtt
     5881 caccgctggg cgtgtatgac ttccagaaac gttccagtct gatcatggtc tcttctgaag
     5941 gcgcgcaaac gctgggcaaa attgctgcga cgttggcgta tggagaaggt ttgcaggcgc
     6001 atgcgcgtag tgccgaatat cgcttgaagt aagaaggttg ccatgagtca atattggagt
     6061 gacatcgttc atcgcctgac gccttatgtg cctggcgagc agcccaagct gcaaaacctg
     6121 gtgaagctga ataccaatga aaatccttat gggcctagtc ccaaggtgat tgcagcgtta
     6181 caggctgaag ctgctgatag tctacgcttg tacccagacc cggactccag ccgcttgaaa
     6241 gtagcgattg ccgaatatca tgggttacag cccaatcagg tgtttgtcgg caatggctcg
     6301 gatgaagtat tggcgcatgt gtttcaagca ttgctcaaac atgagttgcc attgttgttc
     6361 ccggacatta cttatagctt ctatccggtg tattgcggct tatatggcat tgattttgaa
     6421 accgtgccgc ttaattcaaa gtttgaaatt gcagtggatg actatctgcg acccaatggc
     6481 ggtattattt tccccaatcc aaatgcgccg acagggattc ccgtcgcatt aagcgagatt
     6541 gagggtttat taaaagccaa cacaaactcg gtagtcgtga ttgatgaggc ctatgttgat
     6601 tttgggacag agtctgcggt agcgctaatc aataaatatc ctaacctgct ggtgacccat
     6661 acctattcta aatcacgctc gctggctggc ttgcgtgttg gatatgcggt ggggaatgct
     6721 ggtttgattg aggctttaat ccgcattaag gatagtttta actcttatcc tattgaccgt
     6781 tttgctgaag ccggtgcggt tgcagcgtta cacgatgaag attattttca gcaaacacgt
     6841 cagcaagtga tcgcttcacg cgagcagtta gtggccgata tgcaagcatt gggttttgaa
     6901 gtgttgcctt ctggcgctaa ttttatcttt gcacggcatc cggaatattc tggcgaaaaa
     6961 ttggcatcag gcttgcgcga tcaagccgtg atcgtgcggc atttcaaaaa accggcgcgc
     7021 attgcagagt ttttacgtat cactataggt aatgaatcac aaaatcaggc actgatgcag
     7081 gcattgcggt ctttgatgta ggcagaatgt tgctaaaagc aaagtatttg ccgcaatata
     7141 accgctaaca tcaggtaaat gaatgcataa cgacttaaaa aaagtcgtcg caatattgtc
     7201 atttttttca aaatgcggtt atgatgcacg ctattgtaca taatgtgcag tgagggttgc
     7261 gtttgcatga ttgctgcgca ttgattcatt ggattgaaaa tagagcaaac tcggaggaag
     7321 aaaaatggca caaacccaga tggcattgga ctcattggat tttgacgcaa cgatcgcttt
     7381 ggcagctaaa gtagcgccac acgttgatat cctggaaatt ggtacacctt gtatcaagca
     7441 taacggtatc gaattgttga aagcattgcg ttcaaaattc cctaacaaca agattttggt
     7501 tgacctgaaa accatggatg ctggcttcta tgaagctgag ccattctaca aagcaggtgc
     7561 tgacatttgt accgtattgg gtactgctga tatcggtact atcaaaggcg taatcgatgc
     7621 tgctaacaaa tatggcaaag aagctcagat cgacctgatc aacgttaaag ataaaaaagc
     7681 ccgtaccctg gaagtggtca agctgggtgc acacattatc ggtgttcaca ctggtttgga
     7741 tcagcaagcc gctggtcaaa caccatttgc tgacttgggt ctggtatccg gtttgaaaac
     7801 tggcgctaaa gtatctgttg ctggtggtgt taaagcagca acgactaaac aagttgtgga
     7861 tgctggtgct gacatcgtag tggctggtgc tgctatctac ggcgctgctg atccagcagc
     7921 tgctgctaac gaaatcacca agatcgctca cggttctggc gctgctacta aaggtggcaa
     7981 caaattgttg ccatggatca ttgctgcggt agccgcagtg ttggtattct cactgttggg
     8041 caaaaaatct gaagaagctg ccccagctgc tgaagcgcca gctgctgaag aagctgcacc
     8101 tgctgaagct gctccagccg cagaagcacc tgctgccgaa gaagctgctc cagcggaagc
     8161 tgccccagcg gaagctgcgc cagcaactga agaagctaag taatcgattg ctatctttac
     8221 ggatagcttc tggttcaata aaagcagccc gatcgggctg cttttttgtt tttcatgtcg
     8281 gctttttgcc agtccggcaa gacttgctgt aaaatggttg caattcatgc taattctctt
     8341 tgaatacctt cctttctctt atgtcacgtc aagccgaagt cacgcgtaat acgctagaaa
     8401 cacaaattac cgtttcgatt aacctcgatg gtactggtca atccgagttg cacagcggtt
     8461 tgggtttcct ggatcatatg ctggatcaaa ttgcccgcca cggcatgatt gatttaaaag
     8521 tccaggccaa aggcgatttg catatcgatg gccaccacac cgtggaagat attggtatta
     8581 cacttggcca agcgttttcc aaggctttgg gggataagaa ggggttgact cgttatggcc
     8641 atagctatgt cccgcttgat gaggcattga gccgtgtcgc gctggatttg tctggccgcc
     8701 caggtttggc gtttgattgc acttttaccc gcgccatgat tggcgagttt gatgtggact
     8761 taattcatga gttcttccag ggttttgtca atcatgcatt ggtgactttg catattgata
     8821 acctcaaggg gcataacgca catcaccagg ccgaaacgat tttcaaagcc tttggtcgtg
     8881 cgctgcgtat ggcggcgacc cctgatccac gcatggctgg attgatgcca tctaccaagg
     8941 gtgcgttata acgcctgatt gtttatcatg agtcgcttgc aagttgccat tgttgattat
     9001 ggcatgggta atctaaggtc ggtggccaat gcctttaaag ctgtggctcc gactgtttct
     9061 atcgccgtga ccagcgatcc tgcgttgatt gcgcaggctg agcgtgtcgt ttttcctggg
     9121 cagggtgcca tgccggattg tatccgtgag cttgatgccc gtgatttgcg tcaggcagtc
     9181 ctggatgcca cacaacaaaa accgtttcta ggtatttgta tcggtttaca aatgttattt
     9241 gagcattcgg aagaaggtga tgttcctggc ctcggggtct atccagggga agttaaacgc
     9301 tttccgcatg agcgaatgat tgatgaacac ggtgccaagt taaaggtgcc tcatatgggc
     9361 tggagccagg ttcatcaaac ccaggcacat gccatgtggg cagggattcc tgaccaggcg
     9421 cgtttttatt atgtacacag ctattatgtt gctcccgatg agacaagttt aaccgccgct
     9481 accaccgctt atccgtttga tttcaccggt gcgattgcgc gtgataatgt atttgccgtg
     9541 cagtttcacc ctgaaaagag cgccacagca ggcttgcaac ttttgcgtaa ttttgtaaac
     9601 tggaaaccct aaggcgggtt tctggccgtt ttagtcacca ctcgttattt atctattgaa
     9661 aacaacatgt taattattcc agcaattgat ttaaaagatg gtcactgcgt tcgtttgaag
     9721 cagggcttaa tggaagaatc cacagtattt tccgaagacc ctgccgcgat ggccaggcat
     9781 tgggtagata aaggcggcaa acgtttgcat ctggtggacc tgaatggcgc tttcgctggc
     9841 aagccggtga atgaagatgc aatcaagtct atcgttgctg cagttggtgg tgatatccca
     9901 attcaactgg gtggcggtat ccgtgatctg gatacgattg agcgctatct tgacgatggt
     9961 atcagttacg tcatcatcgg tactgctgcc gtaaaaaatc ccgggttttt gcatgaagcc
    10021 tgttatgcat ttcctgggca gattatggtt ggcctcgatg ccaaagatgg caaagtcgcg
    10081 gtggatggct ggtccaagct gactgggcac gatgtgattg atctggcgaa aaaatttgaa
    10141 gattacggcg tggaaagcat tgtgtataca gacattggcc gtgacggcat gctgactggc
    10201 gtgaatattg aagccactgt cgctttggcg caagcattaa ccatccccgt cattgccagc
    10261 ggtggggtca cgaatttgga tgatgtgcgc aatctttgcg cagtgcagga tgagggcatt
    10321 atgggcacga ttaccggtcg tgccatttac gaaggtacgc tggattttgc ggcggcacaa
    10381 cagctggcag accagctgag cggtcaataa tgggctgtgc caagcgcatt attccttgcc
    10441 tggatgtgac cgatggccgc gtggtcaaag gcgttaactt ccttgagctg cgcgatgcag
    10501 gagatcctgt agaaattgcc cagcgttaca atgaccaggg tgcagacgaa ctcacctttt
    10561 tagatattac agccagttct gataaccgtg acctgatctt gcatatcata gagcgtgttg
    10621 cagagcagat ttttattccg ctgaccgtgg gtggcggggt gcgcaaggtc gaagatgtgc
    10681 gccggttact caatgcaggc gccgacaaag tcagtatcaa caccacggct gtcttgaatc
    10741 cacaagttgt ggaggatgca gctggccgtt ttggttcgca atgtattgtg gtggcgattg
    10801 atgctaagaa ggtcgataac cagccatttg aatgggaagt tttcacacat ggtgggcgca
    10861 atcccactgg tttagatgca gtgaaatggg ctcagaaaat ggtcagcctc ggtgcaggtg
    10921 aactgctggt gaccagtatg gaccgtgatg ggaccaaaat tggttttaat aacccgctta
    10981 ataaagcgat cagcgatgct gtcgatgtgc cattgatcgc gtctggtggt gtaggtaact
    11041 tgcagcatct ggtggatggt gtgactgtcg gtggtgcaga tgcggtactg gctgccagta
    11101 tctttcacta tggtgagttt acggtacagc aagccaaaga ctatatgcgt gagcatggat
    11161 tggaagtgcg atgagctggc tggatcaagt gacctggacg gaggacggac ttgtccctgt
    11221 tatcgcccaa gaaaaagaca ctggcaagat tttgatgttt gcctggatga atcgtgaggc
    11281 gttgcaactg acgcgcgata ccggtcacgc cgtgtatttc tcccgctcgc gtaacaagtt
    11341 atggcataaa ggcgaagaat ccgggcatac acagattgtg catgatatcc gtttggattg
    11401 tgataatgat gtcgtcatga tgacggtcga acaactgggg ggcattgcct gtcatactgg
    11461 acgacacaac tgctttttcc agcaactgca aggtgaccag tgggtcacag tagaaccagt
    11521 gcttaaagat ccgaaagcga tttaccatga gtgatggttt gaatggcaat gatgtattaa
    11581 gccggctgtc tgaattgctg gagcagcgta aagctgctga cccatcttct tcttatgttg
    11641 ccaagttgta cgctaaaggc atggacagca ttctgaaaaa aataggcgaa gaggcgacgg
    11701 aaacggtgat cgcagccaag ggcggcaagg cggaagagat tgtttatgaa actgctgact
    11761 tgtggttcca tacgctggtc atgttaagtc atgccggcct cagcgcgcag gatgtcctca
    11821 acgagttggc gcgccgtgaa ggcttgtcag ggattgctga gaaagcgagc agaatagaat
    11881 gagcgcggat tgtgtttttt gcaaaatcgt tgcaggtgac attccttcaa aaaccctgta
    11941 tgaagatgac gatgtggttg tgttccatga catccgtcca ttggcaaagg tacattttct
    12001 gattgtgcct aaaacccata ttgaaacatt gaaagactgt atgccggaac atcagccctt
    12061 attggggaaa atgatgttac tcgctgccaa actggctgct gaacagggct tggttggtta
    12121 taaaac
//
LOCUS       DATMSK010000101         8866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_407197,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000101 DATMSK010000000
VERSION     DATMSK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407197"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..4228
                     /gene="hrpA"
                     /locus_tag="VNZ84_03525"
     CDS             221..4228
                     /gene="hrpA"
                     /locus_tag="VNZ84_03525"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HWU66055.1"
                     /translation="MLADRFPLKRRLNQLRDALKQNKPVDKSLVDIAHKVQASQQRLQ
                     ARLSALPKPEYPPELPVSGKKDEIAAAIAKHQVVIVCGETGSGKTTQLPKICLELGRG
                     VTGMIGHTQPRRIAARSVASRIAQELKSPLGDAVGYKVRFNDKLSESSYVKLMTDGIL
                     LAETQSDKFLNGYDTIIIDEAHERSLNIDFLLGYLKQLLPKRPDLKIIVTSATIDADR
                     FSNHFDGAPVIEVSGRTYPVEIRYRPLGNKTTMVAVEVPVADGLDDIDRFAQDLMGIK
                     RKPPRTETRWLEEDDEEEVMEDAILDTAEDLLRLGDGDILVFLPGEREIRDTADHLRK
                     YQGRSPKLQSIEILPLFARLSIEDQQKIFRPGGQRRIVLATNVAETSLTVPGIKYVID
                     AGLARVNRYSPRAKVEQLQIEKISQAAAKQRAGRCGRVSNGICVRLYSEEDFNQRPAF
                     TDPEILRSSLASVILRMADLRLGDVAEFPFIEAPSSRLISDGYQLLQELGAVTERREI
                     TELGRQMARLPLDPRVARMIIAAKKEGCLREILIIASVLSIQDPRERPMDKREAADNA
                     HAKFKADDSDFMSYLKLWDWYEQALKTKKSNRDLLNQCHQSFLSFLRLKEWRELHQQL
                     LEMVEDMDLKRNDQEASYAQVHRALLSGLLGNIGFKDGDSETYAGARGIRFTIAPGSA
                     LKKNRPKWVMAAELVDTTKLYARVVAKIDPDWIEPIANEGGSRDLTQSDYTDPRWDRK
                     LAQVVAWERVSLYGLTIVPKRRVHYGPINPTEAREIFIREALANMEFDTRAPFFEANR
                     RLIAEVEALEHKARRQDVLVDEYQLFVFYDKVVPPIAGPGHQEPYRIYNGASFEKWRQ
                     DAEKNNPKVLYLTRESLMRHGASAITEVQFPEQMTIDGEAVPLKYRFEPGHVLDGVTA
                     TIPLALLNQLDATVTEWLVPGMVRDKVTALIKALPKTLRRVCVPVPEFVTGFLEAKNP
                     QQPLLPALAEFIQHKTGLKLKPEEFDLAGLDAHHLMNFSVVDEKGRELGMGRDWHLLK
                     KQLGSAAQLTFRTGSPGIEKSGLKQWDFGDLPETLTFSKDGRQLTGYPGLEDNTDSVA
                     VKLFDTPEAATVAHRKGVCRLMRFELKEQMKQLEKGLPGFNQYALLLRAVMNPDQLRE
                     DMLQAIADRAFIAEDELPRTNAEFMQLKARARTRLPAVTEALTRQAQLIANEYQVLTA
                     QLQKMTPVTQKIKRECEQQLQNLLYPGFFSRTPWEQLPHLPRYLKAIKMRIDKYPTSI
                     ERDGRHTQALVQIEQRWQQKVEQLRKAHLPVTSQLQDFAWQLQELRVSLFAQELRTPY
                     PVSIKRLEKLWQEMQ"
     gene            4268..5182
                     /locus_tag="VNZ84_03530"
     CDS             4268..5182
                     /locus_tag="VNZ84_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66056.1"
                     /translation="MQPSPFEPFSTPNWLKPKQRDRVLRIPARLMWAILLSLVFHAIL
                     FWGFLLDLLKPLEEPAQEPLNIVLDLGLPVPKTPKVTPPPQEMPAPEPVPQPKPKPPP
                     KAEPKLPKKIIQSQRPDSPFKVPEPKPQPRQEQPQPTPAPTAEDFSSFIKRKQTDRNA
                     DEMAAKQINDAAAAAERGPSEDERRMKNILNNLKVGTNGLFQIRRMDPYNATFSFKGW
                     VDDYTSANTQYFEVEARSGEDIRLVMIRRMIAIIREHYNGDFDWISNRLGRTVTLSAR
                     NGDNAALEDFLMREFFGNNYKSQDIYGR"
     gene            5195..6730
                     /locus_tag="VNZ84_03535"
     CDS             5195..6730
                     /locus_tag="VNZ84_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU66057.1"
                     /translation="MMPGDFDPPAASNRPSWQHKTALIFFVALGLASVTATGLQYFTA
                     LGVTVITLLYMLCVLWAAYFLRFAQALLTAVVAVLLINYCFIEPRYTFWVASLQSWVV
                     LTVFAILALTVSHAMQQLKWQMQRAQLAAQQSRFFQSLAELLATQVTVAQLLQSACQH
                     VQDVFGWPVSVVALSEAGTLTWLAGEPLPEIQASSVQWALDYQRPIGAGTTDWPGLGA
                     TLLPFGFPQHEVLVVKVSPGEADVHFLRLLTHQVAQATIKLRQQLALAQATQDASEAN
                     FKKTLLTALSHDMRTPLTAILGAVNVLADQQISLAPAQAGQLLSSIQAEAGYLTQATE
                     NILTLVKLDVGASSLHLDWEAPQEVIQHVVHRYAQRTPPLSLQLSMPDASLYGEVLVK
                     MDAVLVAHALANLVDNALQWRSAETPIEIALTVEPPWLVLSVLNHGPGFPPGFVVSAF
                     DARMPSMAGTRGFGLGLSIVQTLMQLHGGELRITTAEDQRTCVSMRLPFVYAADLMQD
                     GKP"
     gene            6727..7422
                     /locus_tag="VNZ84_03540"
     CDS             6727..7422
                     /locus_tag="VNZ84_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU66058.1"
                     /translation="MSQQAGSQVAQPSLLLVEDDPGVYQFLLPALSAHGYRVQHARDL
                     AQARQASGFAMVILDLGLPDGQGEQFIPHVRSYSDVPILVLSARQDELDKVHCLNLGA
                     DDYLMKPFGLQELLARIQVALRRTAIMQMRDHVYQHQSLIINRASGIVTKHGEPLHLS
                     PIEHALLMLLASKPGTIFTHSQLLAQVWGPDYIDDTHYLRIHIGRLRAKLEEDPANPQ
                     TLLTELGIGYRLA"
     gene            7498..>8866
                     /locus_tag="VNZ84_03545"
     CDS             7498..>8866
                     /locus_tag="VNZ84_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWU66059.1"
                     /translation="MEMLSTKKSSLAALALGAIGVVYGDIGTSPLYTIQVIFSEGTGV
                     PLNQANILGAVSSIFWLLMFVVTFKYVFLVLRAHNKGEGGVMALLSLAIAGLRPQGKQ
                     AGVLLLLGVLGAALFYGDSVLTPAISVLSAVEGLQVAAPGLAAQVLPISIGILIGLFW
                     FQRHGTHAVGRFFGPIVIVWFISLASIGIWQMSQAPEILQALQPQYAWQFLQGRGTAV
                     FIAIGAILLAVTGAEALYADMGHFGRPAIQLAWLGLVFPCLTLNYLGQGALLLQHPEA
                     VSNPFYLAFPQPLLIPAVILATLATIIASQAVISGAFSMTRQAIQLGFLPRMRIIHTS
                     ASESGQIYMPAVNWILLLAVIIVCLAFKSSSALASAYGIAVTGTMLITTILLFLVMLQ
                     RWQFPLWGACLLAGMLAAVDSVLLSSASMKFLQGGWFPVALSIGLVLLMTTWKKGRHM
                     LLKGIA"
BASE COUNT         2001 a         2384 c         2446 g         2035 t
ORIGIN      
        1 aagctagggt gcactttctt ttggttactt tatctttggg caagcaaaga aaagtaattt
       61 tcgtactggc aaatcagcac tatcaaataa acaaacttaa gctacttgta agccgccatg
      121 ctgcatgcct ctccccatgc catgttaaaa tctcctccct atgaccgctg tctcaccgat
      181 ccaactcatc acccaactcg aaaaacaact ctcccagtgc atgctggccg accgcttccc
      241 gttgaaacgg cgtttaaacc agttgcgtga tgcacttaaa cagaacaaac ccgtcgataa
      301 atccctggtc gatattgctc acaaagtcca ggcatcgcag cagcgtttgc aggcgcggtt
      361 aagtgcttta cccaaaccag aatatcctcc cgagttgcca gtcagcggca aaaaggacga
      421 gattgcagcg gcgattgcca aacatcaggt ggtgattgtc tgtggtgaaa ccggctccgg
      481 taaaaccaca cagttaccca aaatctgcct ggagttgggt cggggtgtga cgggcatgat
      541 tggccacacc cagccacgcc ggattgctgc gcgttcggtc gcatcacgta tcgcacagga
      601 actcaaaagt ccgttgggtg acgcggtagg ttacaaggtg cgctttaatg acaagctgtc
      661 cgaaagtagt tacgtcaaat tgatgacgga cggtattctg ctggcagaaa cgcaaagcga
      721 taaattcctc aacggctatg acactatcat catcgatgaa gcacatgagc gtagcctgaa
      781 tattgacttc ctgctgggct atcttaagca attattaccc aagcggccgg atttaaaaat
      841 catcgtcacc tcggcgacga ttgatgccga tcgcttttcc aaccactttg acggtgcgcc
      901 ggtgatcgag gtcagtggcc ggacgtatcc ggtcgaaatc cgttaccgtc ctttgggtaa
      961 taaaacgacc atggtcgcag tcgaagtccc cgtggctgat gggctggatg acatagaccg
     1021 ttttgcgcag gacctgatgg gcatcaagcg caagccacca cgcaccgaaa cgcgctggct
     1081 ggaagaggat gacgaagaag aagtcatgga agatgccatc ctcgatactg cagaagattt
     1141 attgcgtctt ggcgatggcg atatcctggt gtttctgccg ggtgaacgcg aaattcgcga
     1201 tacggcagac catttgcgca agtatcaggg ccgttcgccc aagctccagt cgattgagat
     1261 tctgccgttg tttgcacggc tgagtatcga agaccagcag aagattttcc gtccaggcgg
     1321 ccagcgccgc atcgtgctcg ccaccaacgt cgccgaaacg tcattgaccg tgcctggcat
     1381 caagtacgtg attgatgccg gactggcgcg cgtgaaccgt tatagcccac gggccaaggt
     1441 ggagcagttg cagattgaaa aaatctcgca agcggcagcc aaacagcggg cagggcgttg
     1501 cggccgggtg tctaacggta tctgcgtacg cttgtattct gaagaagatt ttaatcagcg
     1561 tccggcattt actgaccccg aaatattacg tagctcactg gccagcgtga ttttgcggat
     1621 ggcggattta cggctgggcg atgtggctga gtttccgttt atcgaggccc catcttcacg
     1681 gttgatcagt gacggttacc agttactgca agagttgggt gccgtgaccg agcggcgcga
     1741 gattaccgag ctgggccgcc agatggcgcg tttgccgcta gacccacgag tggcgcgcat
     1801 gatcattgcc gccaaaaaag aaggctgctt gcgcgagata ttgattattg ccagtgtgct
     1861 gagtatccag gacccgcgcg agcggcccat ggataaacgt gaagcggccg acaatgcgca
     1921 tgccaagttc aaggccgatg attctgactt catgagttac ctcaaattgt gggactggta
     1981 tgagcaggca ctcaagacca agaagtctaa ccgtgatttg cttaaccagt gccaccagag
     2041 cttcctgtcc ttcttgcgcc tgaaagagtg gcgtgagttg catcagcaac tactggagat
     2101 ggtcgaagac atggacctca agcgcaacga tcaggaagcc agctatgcgc aggttcaccg
     2161 cgccttgttg tccgggttgt tggggaatat tggcttcaag gatggtgata gcgaaaccta
     2221 tgccggtgcg cgcggtatcc gttttaccat tgcgccgggc tccgccctca agaaaaaccg
     2281 tcccaaatgg gtgatggcgg ccgagctggt ggatacgacc aaactctatg cacgcgtcgt
     2341 tgccaagata gacccggact ggatagaacc gattgccaac gaaggtggca gccgtgacct
     2401 gacacagagt gattacaccg acccgcgttg ggatagaaag ctagcacagg tcgtagcctg
     2461 ggagcgcgtg agcttgtacg gcctgaccat cgtgcccaag cgccgtgtgc attatggccc
     2521 gatcaacccc accgaagcac gtgagatttt tatccgtgag gcgctggcga atatggaatt
     2581 cgatacgcgg gcaccgttct ttgaggccaa ccgccgtttg attgcagagg ttgaggcact
     2641 ggagcataag gcacgccgtc aggatgtgct ggtcgatgaa taccagctgt ttgtattcta
     2701 cgacaaggtg gtgccgccca ttgcagggcc tggtcatcaa gagccttatc gtatctacaa
     2761 tggcgcgtct ttcgagaaat ggcgccagga tgctgagaaa aacaatccca aggtgttgta
     2821 cctgacacgt gagtcgctga tgcgccatgg cgccagcgcg attaccgagg tgcagttccc
     2881 agagcagatg accatcgatg gtgaagcggt gccgctcaaa taccgttttg agcctggcca
     2941 tgtgctcgat ggcgtcacgg cgacgattcc tttagccttg ctcaaccagc tggatgcgac
     3001 ggtcaccgag tggctggtgc ctggcatggt gcgcgacaaa gtgacagcgc taatcaaggc
     3061 cttgccgaaa accctgcgcc gcgtgtgtgt gccggtgccg gagtttgtga ctggtttcct
     3121 ggaagccaaa aatccgcagc agcccttgtt gccggccttg gctgaattta tccagcacaa
     3181 aaccgggctt aaactcaagc cggaagaatt tgacctcgcc gggctggatg cgcaccacct
     3241 gatgaatttt agtgtggtcg atgaaaaggg cagggagctg ggcatgggcc gcgactggca
     3301 cttgcttaaa aaacaactgg gttctgcggc acaattgacc ttcaggaccg gttcgcctgg
     3361 catcgagaag tctggcctca aacagtggga ttttggtgat ttgcctgaaa cgctcacctt
     3421 tagcaaagat ggtcgccagc tcactggcta tcccggtctg gaagacaaca ctgacagtgt
     3481 cgcggtcaaa ttgtttgata cgccagaagc cgcaacggtt gctcatcgca agggcgtgtg
     3541 ccgattgatg cgatttgaac tcaaagagca gatgaagcaa ttggaaaaag gcctgcccgg
     3601 ctttaaccag tatgccttgc tgttacgcgc tgtcatgaac ccggatcagt tgcgagagga
     3661 tatgttgcag gcgattgctg accgcgcctt tattgccgag gatgaactgc ctcgcaccaa
     3721 tgccgagttt atgcagctca aggcgcgtgc acgtacccgc ttgcccgccg tgaccgaggc
     3781 tttgacccgg caggcacagc tcattgccaa tgaataccag gtactcacgg cgcaattgca
     3841 aaaaatgacg ccagtgacgc aaaaaatcaa acgtgagtgt gaacagcaat tgcaaaacct
     3901 gttatacccg ggatttttca gccgtacacc atgggagcag ttgccacacc tgccgcgcta
     3961 cctcaaggcg attaaaatgc gcatagataa gtatccgacc agcatagagc gtgatggccg
     4021 gcatacacag gcactggtgc aaattgagca gcgctggcag caaaaagtgg agcaattacg
     4081 caaggcacac ctgcctgtaa caagccagtt acaagacttt gcgtggcagt tgcaggagtt
     4141 acgtgtatca ttatttgcgc aggaattaag aacgccttat cctgtctcaa tcaaacgtct
     4201 tgaaaaactc tggcaagaaa tgcaatagtc atgcgtgacc accgctaaca tgtgatgaat
     4261 ccccttcatg cagccgtctc cctttgaacc ctttagtacg cctaactggc tgaagccaaa
     4321 acaacgtgac cgcgtgttgc gtatcccggc acgcctgatg tgggcgatat tgttatcgct
     4381 ggtttttcac gcgattttgt tctggggatt tttgctggac ttgcttaagc cgcttgaaga
     4441 gccagcacaa gagccattga atattgtgct ggacctggga ttgccagtgc ccaaaacgcc
     4501 caaggtcaca ccgccgccac aagagatgcc agcgccggag cctgttccgc aacctaagcc
     4561 aaaaccaccg ccaaaagcgg agcccaagct accgaaaaaa atcattcagt cgcaacggcc
     4621 agatagccca ttcaaggtac cagagcccaa gccacaacca aggcaggaac agccacagcc
     4681 cacgcctgcg ccaaccgctg aggatttttc aagctttatc aagcgcaagc agacggaccg
     4741 caatgccgat gaaatggcag ccaagcagat taatgatgcc gctgcggcag ccgaacgtgg
     4801 cccgtcagaa gatgagcgtc gcatgaaaaa cattctgaac aacctcaagg taggcaccaa
     4861 cggcttgttc cagatcaggc gtatggaccc ctacaatgcg acgttcagtt tcaaaggctg
     4921 ggtagacgat tacacctctg ccaatacgca gtatttcgag gtcgaggccc gcagcggtga
     4981 agatatccgc ctggtgatga tacgccgcat gattgcgatc atccgtgagc attacaatgg
     5041 cgactttgac tggatatcca accgcctggg gcgtacggtc acactctctg cgcgcaatgg
     5101 tgataatgct gcgctggagg acttcctgat gagggagttt tttggcaaca attataaaag
     5161 ccaggatatt tacgggcgct agcgaacacc atccatgatg ccaggcgact tcgatcctcc
     5221 cgcagcgtca aatcggccat cctggcaaca caagactgcc ctgatcttct ttgtcgcgct
     5281 aggccttgca tcagtcaccg cgacgggcct gcaatatttt accgccctcg gtgtcaccgt
     5341 cattaccttg ttgtatatgc tatgcgtact gtgggcggcc tatttcctgc gttttgcgca
     5401 ggccttgctc acggcagtcg tcgccgtcct gctgatcaat tactgtttta tcgaacccag
     5461 gtataccttt tgggtagcga gcttgcaatc ctgggtggtg ttaactgtat ttgccattct
     5521 cgcgctcacg gtcagtcatg ccatgcagca attaaagtgg caaatgcaac gcgcacaact
     5581 ggcagcgcaa caatcccgtt ttttccagtc cctggccgag ctactggcaa cacaggtgac
     5641 ggtcgcacaa ttattgcaaa gcgcttgcca gcatgtacag gatgtctttg gctggccggt
     5701 cagtgtggtg gctttatccg aagctggcac acttacatgg ctggcagggg agccgttacc
     5761 ggaaattcaa gccagcagtg tgcaatgggc cctggattac cagcggccta tcggtgcagg
     5821 taccacagac tggccaggcc tcggcgccac cttgctgcca tttggctttc cgcaacatga
     5881 agtcctggta gtgaaggtaa gccctgggga ggcggatgtg catttcctgc gcttactcac
     5941 ccatcaggtc gcgcaggcca cgatcaagtt acgccagcaa ctcgctttgg cccaggcgac
     6001 gcaggatgcc agtgaagcca acttcaagaa gacactgtta accgccttgt cgcacgatat
     6061 gcgcacgcca ttaacggcga ttctcggtgc agtcaatgtg ctggctgacc agcagattag
     6121 cctggcccct gcacaggccg ggcagttatt aagcagtatc caggctgaag cgggttattt
     6181 gacgcaagcc actgaaaata tcctgacgct ggtcaagctg gatgttggcg ccagcagcct
     6241 gcacctggac tgggaggcgc cacaggaggt catccagcat gtggtgcatc gctatgcgca
     6301 acgcacgccg ccgctctccc tgcaactgag tatgcctgac gctagcctgt acggcgaagt
     6361 gctggtcaag atggatgcag tgctggtggc gcatgcgctg gccaacttgg ttgataatgc
     6421 cttgcaatgg cgtagcgcag agaccccgat tgaaatcgcc ctgacagtag agccaccctg
     6481 gctggtattg tccgtgctga atcacggacc aggcttcccg ccggggttcg tggtctcggc
     6541 gtttgatgca cgcatgccgt ctatggcggg cacgcgcggg tttggtctcg gcttgtccat
     6601 cgtgcagacg ctgatgcaac tgcatggtgg ggagctgcgt attaccactg cagaagacca
     6661 gcggacctgt gtttccatgc gcttgccatt tgtctatgct gcagacctga tgcaggatgg
     6721 caagccatga gccagcaagc gggcagccaa gtggcgcagc catccttgtt actggttgaa
     6781 gacgacccag gggtctacca gtttttgttg cctgcattgt ctgcacatgg ctaccgcgta
     6841 caacatgcac gtgacctggc acaagccagg caagccagcg gatttgccat ggtgatcctc
     6901 gaccttggct tgccggatgg gcagggcgag caatttatcc cgcatgtgcg cagttattcg
     6961 gatgtgccta tcctagtgct ttcggcgcgg caggatgagc tggataaagt gcattgcctc
     7021 aacctggggg cagatgatta cctgatgaaa ccgtttggcc tgcaggaatt actggccagg
     7081 atacaggtcg cgttgaggcg caccgccatc atgcagatgc gtgaccatgt ttaccagcat
     7141 cagtcgctga tcataaaccg cgccagcggc atcgtcacca aacacggtga gccgctgcat
     7201 ttaagcccga tagaacatgc cttgcttatg ttgctcgcca gcaaacccgg cacaatcttt
     7261 acccatagcc agttactggc acaggtatgg gggccagact atattgacga cacccattac
     7321 ctgcgcatcc atattggccg cttacgtgcc aagcttgaag aagaccccgc gaatccgcaa
     7381 accctgctca ccgaactggg catcggttac cgtctcgcct gagatactga cttgtttatg
     7441 cgattgatat accgttagtc gcacactgtt tccatcaatc aaccacaagg ggatatgatg
     7501 gaaatgcttt ccaccaaaaa aagtagtctg gccgccttgg ccttgggcgc gatcggtgtc
     7561 gtctatggcg atatcggcac cagcccgctc tataccatac aagtcatttt tagcgaaggc
     7621 acaggtgtgc cgctcaacca ggccaatatc ctgggcgcag tttcgtccat tttctggtta
     7681 ctgatgtttg tcgtgacctt taaatacgta tttctggtat tacgtgcgca caacaaaggc
     7741 gagggcggcg tgatggcttt attgtcgctg gcgattgcag gcttgcgtcc acagggcaag
     7801 caggcgggtg ttttattatt gctaggcgtg ctgggcgccg ccctgtttta tggcgatagt
     7861 gtgcttacac cagccatctc agtgctgtct gcggttgaag gcctgcaagt ggctgcccca
     7921 gggctggctg cccaggtgtt gcccatttcg ataggcatcc tgatcggcct gttctggttc
     7981 cagcggcatg gtacccatgc cgtgggccgt ttttttggcc ctatcgtgat tgtttggttt
     8041 atctcgctcg ccagcatagg catctggcaa atgagccagg cgccggaaat attgcaggca
     8101 ttgcagccgc aatatgcctg gcaattcctg caaggccgtg gcacggccgt gtttattgcc
     8161 attggtgcga ttctactggc ggtgaccggg gcagaagccc tctatgccga tatgggtcac
     8221 tttggccgcc cagccatcca gttggcctgg ctggggctgg tgtttccctg tttaaccctg
     8281 aactacctgg ggcagggcgc attactgctg caacatcctg aggctgtgag taatccgttt
     8341 tacctcgcgt ttccgcaacc cttgctgatt ccagccgtta ttctcgctac gctggcaacg
     8401 atcattgctt cacaagcggt gatttccggc gcattttcca tgacccgtca ggcgattcaa
     8461 ttgggttttt tgccacgcat gcgcattatc catacctctg ccagtgaatc gggccagatt
     8521 tatatgcccg ccgtgaactg gatcttgcta ctggccgtga ttattgtgtg tctggcattc
     8581 aaaagctctt cggccctggc ctctgcttat ggcattgccg ttacaggcac catgctcatc
     8641 accaccatcc tgttgtttct tgtcatgctt cagcgttggc agtttccgct ttggggtgct
     8701 tgtttgctgg cagggatgtt ggctgcagtg gatagcgtgc tgctcagctc cgcttcgatg
     8761 aaatttctcc agggcggctg gttcccggtg gccctgagca tcggattggt gttgctgatg
     8821 accacctgga aaaaaggccg tcatatgcta ttgaaaggga tcgcag
//
LOCUS       DATMSK010000102         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_430450,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000102 DATMSK010000000
VERSION     DATMSK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430450"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VNZ84_03550"
     CDS             complement(<1..270)
                     /locus_tag="VNZ84_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HWU66060.1"
                     /translation="MHKIMTISLLIAALGTSGCANMSETQKGTAKGAGIGAAAGGVIG
                     AVAGGGKGAVIGAAVGAGAGAIAGNIWSKKMEAQKQEMEQATAGTG"
     gene            650..1153
                     /locus_tag="VNZ84_03555"
     CDS             650..1153
                     /locus_tag="VNZ84_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66061.1"
                     /translation="MVPPIILDIEASGFGVGSYPVEIGYANADGTTWSAQVQPHADWL
                     HWDKEAEKLHQQSRQSLEAHGQTVQDIAAHLNQVFAGKTVYTDGWYQDFVWLHNLYEA
                     AGLSPKFKLEDLSLTLSPEQKSAWHETKQIIRENLALQQHRASTDAKVLQLTWLRTAQ
                     AAAALAD"
     gene            1240..2334
                     /gene="earP"
                     /locus_tag="VNZ84_03560"
     CDS             1240..2334
                     /gene="earP"
                     /locus_tag="VNZ84_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HWU66062.1"
                     /translation="MSSFDSTRTRWDMFCKVVDNFGDVGVCWRLARQLADEHGIDIRL
                     WVDDIALASRFAGGGHAGVTLQQWTEHADFSHAAKVVIETFGCGLPEAYQQSMLLQHS
                     TWVNVDYLSAESWVEGFHAQPSPQPSGLVRYFFYPGFTQATGGLLREASLIGLQTQSS
                     WQALGLEPAPDKLNVSLFCYAHAPLAALVDSLQASSQSVRVLVPQTVAPMLAQVLNTP
                     ILSVGDRVQRQQCEFVVIPFLSQPDYDRLLYLCDLNFVRGEDSWIRALWAGKPMIWLP
                     YQQSEETHLLKLKAFMQHYLAEAETPLATIIQTAMQAWAQGHWQATDWEALLSVLPQF
                     RQHAQAFKLRQSQQVDLATHLVIFVQKIAK"
     gene            2534..>3071
                     /gene="efp"
                     /locus_tag="VNZ84_03565"
     CDS             2534..>3071
                     /gene="efp"
                     /locus_tag="VNZ84_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWU66063.1"
                     /translation="MKIAQELRVGNVVMIGNDPMVVVKTEYNKSGRNAAVVKMKMKNL
                     LTEAPSENVYNAQDKFDVIVLEKKEVTFSYFADPTYVFMDAEYNQYDVDAEFMEDALP
                     YLEDGMPCEVRFYEGKAISIELPTTVVREITYTEPAVKGDTSGKVMKPAKIASGFEIP
                     VPLFVNQGDKIEIDTRTGE"
BASE COUNT          768 a          727 c          783 g          793 t
ORIGIN      
        1 cccggtgcca gcagtggcct gctccatttc ctgtttctgg gcttccattt ttttggacca
       61 gatattgccg gcaatggcac ccgcgccagc gcctaccgcg gcaccaatca ccgcgccttt
      121 accaccacct gcaaccgcac caatgacacc gcctgccgct gcgccaatac cagctccttt
      181 ggctgtccct ttttgagttt cgctcatgtt tgcgcaaccg gaggtaccca gtgcggcgat
      241 taacagagat attgtcatta ttttatgcat tgttttccct tacgtagtgt gaatggcttt
      301 agagcacttc tataaattca acttccgttg ccatactgtg ttgcttataa aaaatgtatc
      361 ctcacatatt atctatatgc tgcgtccaga tgatttaagc aggcaatgcg tgaaacgcat
      421 gctcgcaaat tttttaatcg cgccttgtct ggctgagcag tttgaattta tagaagcagt
      481 ttcagtttgt ccagtcgggc tcggggaagt tcagtcccca gtctagaaga attgcgtata
      541 attctcactt aattaaagca cgctgctttt ctgccgttga atagccagat ggtaaatagt
      601 agcagcaagt gcagataaat ttacagagaa gatacaccca ggtaagttga tggttccacc
      661 aatcatatta gatattgaag catccggttt cggcgtgggt agttacccgg tcgagattgg
      721 ttacgccaat gcagacggaa ccacctggag tgcgcaggtg cagccgcatg cagactggtt
      781 gcattgggat aaagaagctg aaaagctaca ccagcaatcc cgtcaatcgt tagaagcgca
      841 tggccagacc gtacaggata ttgctgcgca cctcaaccag gtgtttgctg gcaaaacagt
      901 gtataccgat ggctggtacc aggattttgt ctggttacat aatctctatg aagcggcggg
      961 tttaagccct aaatttaaac tcgaagattt aagcttaacc ctgtcgcctg aacaaaaatc
     1021 agcctggcac gagaccaagc aaataatccg tgaaaacctg gcattgcagc aacatcgggc
     1081 gtctaccgat gccaaggtat tgcaactgac ctggctccgc actgcgcaag ccgcggcagc
     1141 ccttgcagat taatctatcg ctgacgcccg agctgtgatg gctatcgttc gggtagacta
     1201 gccaaacttt catcctcgcc tgagtttata agcctgatca tgtcctcttt tgactccacc
     1261 cgtacccgct gggatatgtt ctgcaaagtg gttgataact ttggggatgt tggcgtttgc
     1321 tggcgtctgg cgcgtcagct ggcagatgag catggcatcg atatccgctt gtgggtggat
     1381 gatattgcat tggcgtcacg gtttgcaggg ggcgggcacg caggcgtgac tttgcagcag
     1441 tggactgaac acgcagactt ttctcatgca gccaaggtgg tgatagaaac ttttggttgc
     1501 ggcttgcctg aggcttatca gcaaagcatg ttgttgcagc actccacttg ggtgaatgtc
     1561 gactatttgt cggcggaaag ctgggtggag gggtttcatg cgcaaccatc ccctcagcca
     1621 agtggcctgg tgagatattt cttttatcct gggtttacgc aagccacagg tggtttgtta
     1681 cgtgaagcca gcttaatcgg gctgcaaaca caatccagtt ggcaagcgtt ggggctggaa
     1741 cccgcgccag acaagttgaa tgtgtcttta ttctgttacg cccatgcacc cttggctgcg
     1801 ttggtcgata gcctgcaggc atcaagccaa tctgtacgag tgctggtgcc acaaacggtt
     1861 gcccccatgt tggctcaagt actcaataca cccattttat cggtagggga tcgcgtacag
     1921 aggcagcaat gcgagtttgt cgtcatccca tttttatcac agcctgatta tgaccgcttg
     1981 ctctatctgt gtgacttgaa ttttgtacgc ggtgaagata gctggattcg tgcgctatgg
     2041 gctggcaagc ccatgatctg gttaccttac cagcaaagcg aagagacaca cctgctgaaa
     2101 ttaaaagcgt ttatgcaaca ttacctggca gaagcagaaa caccgctagc tacgatcatc
     2161 caaacggcta tgcaggcgtg ggcgcaaggc cactggcaag caaccgattg ggaagctttg
     2221 ttgtctgtat tgccgcaatt caggcagcat gcgcaagcgt ttaagttgcg ccagtcacaa
     2281 caagtcgatt tggccactca cttggtgatt tttgttcaga aaatagcgaa gtgagtgagc
     2341 gagcggaata aagtgcgagg cgcacggaac gcaggaagca aggcagtaca taaagtacga
     2401 cttgtttatg agtaccgcgt aacgcagcaa tttgcgcgcg tagccactta attccttgtt
     2461 ttcttgagaa atgtccggca tatcgggtat aattcggcgc tttcaaatcc cgcatacaaa
     2521 ttaaggctta tttatgaaaa tcgctcaaga actccgcgtt ggcaacgtag tgatgattgg
     2581 caacgacccg atggtggttg taaaaaccga atacaacaaa tccggccgta atgcggctgt
     2641 ggtgaaaatg aaaatgaaaa acttgttgac ggaagcgcca agcgaaaacg tttacaacgc
     2701 ccaggacaag tttgacgtga ttgtgttgga aaagaaagaa gtgactttct cttactttgc
     2761 tgatcctacc tatgtattta tggacgctga gtacaaccag tacgacgtag atgccgagtt
     2821 tatggaagat gcgctgccat accttgaaga tggtatgcca tgtgaagtgc gtttctacga
     2881 aggcaaagcg atttctatcg aattgccaac cactgttgtg cgtgagatca cttacaccga
     2941 gccagctgtg aaaggtgata cttccggtaa agtgatgaaa ccggccaaga tcgcttccgg
     3001 tttcgaaatt ccagtgcctc tgtttgtcaa ccagggcgac aaaatcgaga tcgatacacg
     3061 tactggtgaa t
//
LOCUS       DATMSK010000103         4967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_243121,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000103 DATMSK010000000
VERSION     DATMSK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243121"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VNZ84_03570"
     CDS             complement(<1..1503)
                     /locus_tag="VNZ84_03570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWU66064.1"
                     /translation="MIAASASILAMYLAESAKQFVLKRDQQLALAGGAVALGAAVWSM
                     HFIGMLAFELCATVKYAKWTTILSILPALIASWVAMRWLFDMRKGMLHLLLGGTLTGA
                     GIGIMHYSGMLAMQTAAAIGFELWGVLLSVFVAVVLATLALWFRHICDGFGQWRAWRH
                     VIGGVVMGAAITTMHYVGMSSARFIGIAEFSVPVFTVDRWYLSMLIALGSMALIGLMT
                     AQNMLAKQRQLGRELQFKEHQMRVIFQNAIDAIVITDHAGIIQTVNRSFEGLFGFNAQ
                     YALGKHVSMLIPEWNELSIPNQLRRQTDVTDQWVVECNARHADGHEIPLRSALVSIRE
                     HDQDLYVGFLMDMTMFHAQQKNLERMVTEDALTGLMNRRGLIKIMQQTVTLPAKDRPG
                     IALLFIDLDGFKAVNDKYGHTSGDEVLIEVSRRVQRTLRQDEVVCRFGGDEFVIMLRY
                     LRDLDALTKTVADKVKLAIAPPILLSNGIEVKVGCSIGVANALPGKLEDID"
     gene            1793..2542
                     /locus_tag="VNZ84_03575"
     CDS             1793..2542
                     /locus_tag="VNZ84_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWU66065.1"
                     /translation="MKQFGIYIIGDEILSGKRQDAHLGKVISLLSARGLQLSWAHFIG
                     DIPEQITSLLKASMARGDVVFSFGGIGATPDDHTRQCAAAAAGAPIERHAGAVANIEA
                     QYGETAYPKRVLMADFPQGCDLIPNPVNRVAGFSIHEHYFVPGFPEMAHPMIEWVLDT
                     YYSHLFHTNDYLEQAIVVTEAGESDLIDLMNAMLGRYPMLKLFSLPRTNQRRTTEVGM
                     KGQSALVKQAMLDLKAGVSALGYPWTDVLES"
     gene            2553..2810
                     /locus_tag="VNZ84_03580"
     CDS             2553..2810
                     /locus_tag="VNZ84_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HWU66066.1"
                     /translation="MNCRPQCGACCIAPSINSPIPGMPLGKPAGVPCIQLDELLRCKL
                     FGQPERPAFCGGLQPAVDMCGETREQAISWLNGLESATQPD"
     gene            complement(2818..3609)
                     /gene="lgt"
                     /locus_tag="VNZ84_03585"
     CDS             complement(2818..3609)
                     /gene="lgt"
                     /locus_tag="VNZ84_03585"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWU66067.1"
                     /translation="MFTHPGFNPIALQIGSFGIHWYGLMYLVGFFGGLWLGQYQVKRL
                     RKIDPASPWQERDPDDMLFYIALGVVLGGRLGYVLFYQPAYFLQHPLEIFALWQGGMS
                     FHGGFIGVMVAMALFARKRGVAWLQLMDFVAPLVPIGLGAGRLGNFINAELWGRPTNA
                     DWGMVFPAVDQVARHPSQLYEFALEGIVLFLILWTFAGKKRRVGAISAMFLLCYGSFR
                     FIVEFTREPDSFLGLLSLGLSMGQWLCVPMILVGLAMMRWAYRKQ"
     gene            complement(3654..4916)
                     /locus_tag="VNZ84_03590"
     CDS             complement(3654..4916)
                     /locus_tag="VNZ84_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU66068.1"
                     /translation="MTAPSLKHIAFASFVGTAIEFYDFYIYAMAAALVIGQVFFPASD
                     PSMQSLNALLTFGLAFIARPLGALLFGHFGDRIGRKSTLAVSLLVMGTSTFLIGLLPA
                     YASWGVWAPICLCVLRFVQGIGLGGEWAGAALLATEYAPEGKRGWYGMFPQLGPSIGF
                     LLATLTFLGLSLGLSDAQFALWGWRVPFIISAALVMIGLYVRFKLAETPVFAQAQAAH
                     HLHRWPLKTLLLQHGRSIVLGALAMSVCYHLFYTATVFCLSYGTQTLHIARPVFLALL
                     CVAVIGMAIATPLAAWLADRVGRRPVLMSGQLFAVALGFALMPMVGSGQWLWILLFLF
                     AALFLMGVTFAPMGAFLPEQFPVAVRYSGAGLSYQLGGILGASFAPAIAQALVQQGGL
                     SWVGGYMSCMALISLLAVWQMPSSVQSD"
BASE COUNT         1429 a         1400 c         1151 g          987 t
ORIGIN      
        1 atctatatct tcaagcttac ctggcagggc attggccact ccaatactgc accccacctt
       61 aacctctata ccattactca gaaggatggg gggagcaatg gccagtttaa ccttgtcggc
      121 caccgttttg gtgagggcat ccaaatctct caaataacgt aacatgatca caaattcgtc
      181 ccccccaaag cggcaaacga cctcatcctg tcgcaaggta cgctgaacac gacgagaaac
      241 ctcaatcagg acctcgtcgc cagacgtatg gccgtacttg tcattgacag ccttgaaccc
      301 gtccagatca ataaatagca atgcaatccc gggtctatct tttgcaggca gtgtcaccgt
      361 ttgctgcata atttttatca ggcctcgacg attcatcagg ccagtcaaag catcttcagt
      421 caccatacgc tcaagatttt tttgctgggc atgaaacatg gtcatatcca tcaagaaacc
      481 cacatacaga tcctgatcat gctctcgaat cgacacgagt gcgctcctca gtggaatttc
      541 atggccgtcc gcatgccgag cgttacactc caccacccac tggtcagtca catcggtctg
      601 gcggcgtaat tgattgggaa tactcaactc attccattcc ggaatcaaca tggacacatg
      661 tttacctaat gcatattgcg cattaaaacc aaacagcccc tcaaacgaac gattcacagt
      721 ctggataatg ccagcatgat cggtaatcac aatggcatca atcgcatttt gaaaaataac
      781 ccgcatctgg tgctctttaa attgcaactc acggcccaac tgtcgttgct tggcgagcat
      841 attctgtgct gtcatgaggc caatgagcgc catggacccc aatgcaatca acatggataa
      901 gtaccatcga tccaccgtaa acacaggcac ggaaaattct gcaatgccaa taaagcgggc
      961 agatgacatc cccacatagt gcatggtagt gatggcagcc cccatgacca cgcccccaat
     1021 cacatgccgc caggcacgcc actgaccaaa accatcacag atatggcgaa accatagagc
     1081 gagcgtagca aggaccaccg cgacgaagac tgacaaaagt accccccaca actcgaagcc
     1141 gatggcagcc gccgtttgca tggcgagcat accgctgtaa tgcatgatcc caatgccagc
     1201 gccggtcaga gtacccccca gcaaaaggtg caacattcct ttacgcatat caaacaacca
     1261 gcgcatcgcc acccacgagg cgatcaatgc tggcaaaatg gaaaggatag ttgtccactt
     1321 agcatacttt accgtggcgc ataactcgaa agcgagcata cctatgaagt gcatgctcca
     1381 tactgcggcc cctaacgcta cagcaccgcc tgcaagggcg agttgctgat cccgtttgag
     1441 cacaaactgc ttggcacttt ctgccagata catggctaat atcgaggcac ttgcagcgat
     1501 cacaaatgac aaaaccacca ggcttgtgtc atatcgatga agaagctcac tcgcgtcgga
     1561 agggggacca gcaaacggga aattaatcat ttcatcaggg ccatttgaaa tgagacaaga
     1621 aaagcttata tcaaatcgcc gggatgcaaa aggctaaata gtctataaaa acgcattcga
     1681 ttgtattcaa tgctattatt tgcgcaaaaa acatggcggg tacatgtagc agcaaaatgc
     1741 actgtatatg cgccgaatat atatttggta agacttactc aaagaatcaa gcatgaaaca
     1801 gtttggtatc tatattattg gtgacgagat tctttccgga aaacgtcagg atgctcatct
     1861 tggcaaagtg atctcattgc tctctgcacg cggcctgcaa ttaagctggg cacatttcat
     1921 cggtgacatc cctgaacaaa tcacgagcct gctcaaagcc agtatggcac ggggtgatgt
     1981 ggtattcagt tttggcggca tcggcgcgac accagacgat catacgcgcc aatgtgctgc
     2041 ggcagcggca ggcgcaccga ttgagcggca tgcgggtgca gttgcaaata ttgaagcgca
     2101 atacggcgaa accgcttacc ctaagcgagt cttgatggct gattttccgc aaggctgcga
     2161 tctgattccc aatcctgtaa accgtgtggc cggattcagc attcatgagc attactttgt
     2221 gccagggttt ccagaaatgg cacatcccat gattgaatgg gtgctcgaca cgtattacag
     2281 ccacctcttt cacaccaatg actatctgga acaagcaatt gtggtgaccg aggccgggga
     2341 aagtgacttg attgacctga tgaatgccat gcttggccgc tatcccatgc tcaagctctt
     2401 cagtctgccg cgaactaatc agcgccgtac gacagaagtg ggcatgaaag gacaaagcgc
     2461 gctggtcaaa caagcgatgc tagacctgaa agccggtgta tccgcgcttg gttacccttg
     2521 gacagatgtg ctggaatcat aagccagacc atatgaattg ccgtccgcaa tgtggtgcct
     2581 gttgtattgc accttccatc aacagcccga ttccaggcat gcctcttggc aagcccgcgg
     2641 gcgtgccttg cattcaactg gatgaattat tgagatgcaa actgtttggt caaccggagc
     2701 gtcctgcgtt ttgtggtggg ctacaaccag ccgtcgacat gtgtggcgaa acgcgtgaac
     2761 aagcgattag ctggctgaat gggctggaat cagccactca gccagattga aagtggatta
     2821 ctgtttacga taagcccagc gcatcatggc cagcccaacc agaatcatag gcacgcacag
     2881 ccattgcccc atactcagtc ccagcgacaa caagcctaag aagctatccg gctcccgggt
     2941 aaattcgaca ataaagcgga agctgccata gcacagcagg aacatggccg agatggcgcc
     3001 tactcggcgc ttcttgccgg caaacgtcca caaaataaga aacagcacga tgccttcaag
     3061 cgcaaactca tacagttgcg acgggtgacg ggcaacctga tcgacggcag gaaacaccat
     3121 cccccaatcg gcattggtcg ggcgccccca caactcggca ttgataaaat tacccaagcg
     3181 gcctgcacca agcccaatcg gcaccaaagg tgcgacaaaa tccattaatt gcagccatgc
     3241 cacgccacgc ttgcgagcaa acagcgccat cgcgaccatc acgccgataa acccgccatg
     3301 gaacgacata ccgccttgcc ataaggcaaa aatctccagt gggtgctgca agaagtaagc
     3361 aggctgataa aacagcacat atcccaacct gccgcctagt accacgccca acgcgatata
     3421 aaacagcata tcgtccgggt ctcgctcctg ccagggagaa gcggggtcta ttttgcgcaa
     3481 acgcttgacc tgatattgcc ccagccacaa accgccaaaa aatccaacca ggtacatcaa
     3541 gccgtaccag tgaatgccaa aactgccgat ttgcaatgca atggggttaa atcccggatg
     3601 ggtaaacatg ccgtatcacc gtgaaaaaaa ttgcattata cctgctataa gagtcagtca
     3661 ctttgtactg atgaaggcat ttgccacacg gctagcaagc tgatcaatgc catgcatgac
     3721 atgtagccgc ccacccatga cagccctccc tgctgcacca aagcctgcgc aatggcagga
     3781 gcaaatgacg cacctagaat accgcccaac tggtaactca accctgcccc gctatacctg
     3841 acggccaccg gaaactgttc aggcaaaaac gcacccatgg gcgcaaaggt cacccccatt
     3901 aaaaatagcg ctgcaaacag aaagagtaaa atccatagcc attggccact ccccaccata
     3961 ggcatcaggg caaagcccaa tgcgacggcg aataattgcc cactcatcaa tacggggcgc
     4021 cgcccaaccc ggtctgccaa ccatgcagca agtggcgtcg caatagccat ccctatgacg
     4081 gcaacacaca gtagcgcaag aaacactgga cgcgcaatat gcaaggtttg cgtgccataa
     4141 ctcaggcaaa aaaccgtcgc cgtgtaaaac aggtggtaac acacgctcat ggccagcgcc
     4201 cccagtacaa ttgaccgtcc atgctggagt aacaatgtct ttaatggcca gcgatgcaag
     4261 tgatgtgctg cctgtgcctg ggcaaatacc ggtgtttcgg ccagtttaaa cctgacatat
     4321 aaaccaatca tcaccagtgc ggcactgatg atgaaaggca cccgccagcc ccagagggca
     4381 aattgcgcgt cgcttaatcc caggcttaaa cccagaaacg tcagggtcgc cagtaaaaag
     4441 ccgattgaag gccccagttg gggaaacatg ccataccagc cacgcttgcc ttcaggcgca
     4501 tactcggtag cgagcaaagc tgcaccagcc cattccccac ccaaccctat cccctgcaca
     4561 aaacgcaaaa cacacaggca tatgggtgcc cagacacccc aacttgcata agcaggcaac
     4621 aaaccaatca aaaaggtgga tgtgcccatc accagcagag ataccgccag tgtagattta
     4681 cggccaatac ggtcgccaaa atggccaaac aatagtgcac ccaatggtcg agcaataaat
     4741 gccaggccaa acgtgagcaa ggcattcaaa gattgcatgg agggatcact ggcagggaaa
     4801 aatacctggc cgatgaccag cgccgccgcc atggcgtaaa tataaaaatc gtagaactcg
     4861 atggccgtac caacaaagct ggcaaaggcg atatgtttta aggagggcgc tgtcatgacg
     4921 gcatcttaag ggcatagacc agttttgcct agccaataaa aaaaccg
//
LOCUS       DATMSK010000104         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_314143,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000104 DATMSK010000000
VERSION     DATMSK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314143"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VNZ84_03595"
     CDS             complement(<1..890)
                     /locus_tag="VNZ84_03595"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWU66069.1"
                     /translation="MTQARQAGVLMHITSLPEGNLGPDAYHFVDKLVEMGVSIWQTLP
                     VNQPHDDQSPYQSVSAHAGHTGMISAQQLLNSGWCLPNEVTLPLSSLLSKVMSRLQSG
                     EVLPRTDGGGISWPGFARFCQTQQYWLEDFALFLSLREQHAHQSWHQWPQVYRMHDIL
                     ALQAYSAANQEKLTLIKFTQYVFFQQWAQLKTYANQHGIQLFGDIPIFVAYDSADVWA
                     HPDWFKLDPDLNMTVVAGVPPDYFSETGQRWGNPHYDWQAMQADGFSWWLGRIDTQSQ
                     LFDVLRIDHFRGLQAAWEIPV"
     gene            complement(921..2624)
                     /locus_tag="VNZ84_03600"
     CDS             complement(921..2624)
                     /locus_tag="VNZ84_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HWU66070.1"
                     /translation="MSDKKLLLNLYWHMHQPDYRDLVTGDYVLPWTYLHALKDYSDMA
                     YHLEQNPAARVTFNFVPILVEQLQDYAEQAHSGVWRDPLLHLLGNPDLDHISDAECQL
                     IVDSCFKAHHEKMLSPFPHYQRLLKLYELVAPLMQSGQFHYLSAQYKADLLVWYHLAW
                     TGEGLRRKNKTVQALMQKGVLFTFEDRLALLGVISETLQGILPRYQALLAKGQIEMST
                     TPYYHPILPLLIDLKSTHDAMPKAPLPQATTYPDGYTRAISHVEAAQQYHEQVFGHKA
                     TGMWPAEGAVSHAALSLLAEEGVAWAATGQGVLANSLRKLGLEHDQPQQYLYQPYKIT
                     NGQQDIVCFFRDDQLSDKIGFEYSKQYASEAVNDFVQSLENILSNSEGTPVVSVILDG
                     ENAWEYYPYNGYYFLQELYQALVDHPDIQMTTFSDILAMQQAQQLPEPALLNDICAGS
                     WVYGTFSTWIGEPAKNRAWDLLCEAKRQYDQHVHGLSLAAKEKCQQQLALCEGSDWFW
                     WFGDYNNAQSVQSFDQLYRRNLSNLYHLLGLTPPAHLSQPISLGGGDAENAGTMRRGQ
                     S"
     gene            complement(2663..>3582)
                     /locus_tag="VNZ84_03605"
     CDS             complement(2663..>3582)
                     /locus_tag="VNZ84_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWU66071.1"
                     /translation="IDLLRENGGEYILVLAGDHIYKMDYGKMLATHARSNADMTIACI
                     NVPLEDAKGFGVLEVDGTDRVVAFDEKPANPKPTPNNPEVAFASMGIYVFNAKFLYEQ
                     LIRDAYARNSNHDFGQDIIPYIIKKYKVQAHRFTDSCVGAQSDGYYWRDVGTIDAYWE
                     ANMELTKVIPELNLYDSEWPIWTYQEQLPPAKFVFKDEGRTGKATDSLVSGGCLISGS
                     CVSNSVLFSGVHVADYSDLYGAVVLPKVKINEGVVMRNAVIDRGCEIPAGMHIGVDLE
                     RDRKRFYVSEKGIVLVTPDMLGQDLYRVK"
BASE COUNT          922 a          944 c          815 g          901 t
ORIGIN      
        1 actggaattt cccacgctgc ctgcaagcca cgaaagtggt ctatacgcaa tacatcgaat
       61 agctgggact gggtatctat gcggcccagc caccatgaaa atccatcagc ctgcatggct
      121 tgccagtcat aatgcgggtt accccagcgc tgccccgttt cggaaaagta atctggcggc
      181 acgcctgcga ccacagtcat attgagatca ggatccagct tgaaccagtc ggggtgtgcc
      241 cagacatcgg cactatcata agcaacaaaa atcgggatat cgccaaacaa ctggatccca
      301 tgctggttgg cataggtttt aagctgcgcc cattgctgga aaaacacata ttgcgtaaat
      361 ttgatgagtg tcagtttttc ctgattggca gcgctataag cctgcaacgc caagatatca
      421 tgcatgcggt aaacttgcgg ccattgatgc cagctttgat gcgcatgctg ctctcgcaaa
      481 ctcaaaaata gcgcaaaatc ttccagccaa tattgttgcg tctggcaaaa acgggcaaac
      541 ccgggccagc taatacctcc accatctgtg cgtggtagca cttctccaga ctgcaagcgt
      601 gacatgacct tagacagcaa agaggataat ggaagagtca cttcatttgg cagacaccag
      661 ccactgttta ataactgttg cgcgctgatc atgccggtat gtccagcatg cgccgacaca
      721 ctctggtaag gtgactggtc atcatgtggc tgattgacgg gcaaggtttg ccaaatggat
      781 acgcccattt cgaccagctt gtctacaaag tgataggcat ccgggcccag attgccctca
      841 ggtaaggacg tgatgtgcat caacacaccc gcttgtctcg cttgtgtcat gtgattggca
      901 ttcaggtaat ttaaaatcgg tcaggactgt ccgcgacgca tggtgcctgc attctcagca
      961 tcccctcctc ccaggctgat gggctgggac aagtgagcag gtggtgtcaa gccaagcaaa
     1021 tgatagagat tactcaggtt acgtcggtat aactggtcaa aactttgaac gctttgcgca
     1081 ttgttgtaat cgccaaacca ccaaaaccag tccgatcctt cacacaatgc taactgctgt
     1141 tggcattttt ctttagccgc tagagaaagc ccatgcacat gttggtcata ttgccgtttg
     1201 gcttcgcata ataaatccca tgcacggttt ttagcaggct cgcctatcca ggtgctaaag
     1261 gtgccgtaaa cccagctccc ggcacaaata tcattgagca gtgcaggctc aggtaattgc
     1321 tgtgcttgct gcatggccag gatatcgcta aacgtcgtca tctgtatatc aggatgatca
     1381 accagcgcct ggtaaagttc ctgtaaaaag taatatccgt tgtaggggta atattcccag
     1441 gcattttcac catcaagaat cacactgacg acgggtgtcc cctcgctgtt actgagaatg
     1501 ttttccagtg actgcacaaa atcattcacc gcctcgctgg catactgttt ggagtactca
     1561 aagccaattt tgtctgaaag ctggtcatca cggaaaaaac agacaatatc ctgctgacca
     1621 ttcgtgatct tatatggctg gtagagatat tgttgcggct ggtcatgttc taatcccagt
     1681 ttgcgcaaac tgttcgccag caccccttgc ccggtagccg cccaagcgac tccttcttca
     1741 gccagtaacg agagtgcagc gtgtgagacg gcaccttccg caggccacat gcctgtcgct
     1801 ttgtggccaa acacctgctc atgatattgc tgggctgcct ccacatgcga aattgcgcgc
     1861 gtgtatccat caggataagt tgtggcctgc ggtaacggtg ctttaggcat ggcatcatgc
     1921 gtgcttttca ggtctatcag cagtggcaat atcggatggt agtaaggggt cgtagacatt
     1981 tcgatctgcc cttttgccag caatgcctgg tagcgcggca aaatcccttg caaggtttca
     2041 ctgatcacgc ccagcaacgc caggcgatcc tcaaaggtaa acagtacgcc tttttgcatt
     2101 agtgcctgca ccgttttatt tttacgacgc aaaccttcgc ctgtccatgc cagatggtac
     2161 caaaccagca aatctgcttt atattgcgca gaaagatagt gaaactgacc actttgcatc
     2221 agcggagcga ccaactcata tagtttcaac agtcgctggt aatgcggaaa agggctcagc
     2281 attttttcat gatgcgcctt gaaacaacta tccactatca actgacactc agcatcagaa
     2341 atatggtcaa ggtcgggatt acccagcaag tgcaataatg gatcacgcca tacaccgctg
     2401 tgtgcctgct cggcataatc ctgcaactgc tcgaccagaa tcggtacgaa gttgaaggtc
     2461 accctggccg caggattctg ctccaggtga taggccatgt cactataatc tttgagcgca
     2521 tgcagatagg tccagggcaa tacataatca ccagtgacga ggtcacggta gtcgggttga
     2581 tgcatgtgcc aatagagatt taacaacagt tttttatcgg acatccttca atctctttcc
     2641 cttattactc atttacactg acctacttca cacggtataa atcctgacca agcatatccg
     2701 gcgtcaccag tacgatgcct ttttcagaga cataaaagcg tttcctgtca cgctcaaggt
     2761 caacgccaat gtgcatgcct gccggaatct cgcaaccacg gtcaatgact gcattgcgca
     2821 tgacgacgcc ttcattgatt ttaactttag gcaataccac ggcaccgtac agatctgagt
     2881 agtctgcaac atgcacacct gaaaacaaca cagagttcga aacacaagaa ccgctgatca
     2941 ggcatcctcc cgaaaccagt gaatccgtcg ctttacccgt acggccttcg tccttaaaca
     3001 caaatttagc cggagggagt tgctcctgat aagtccagat gggccactcg ctatcataca
     3061 aattgagctc agggatgact tttgtcagct ccatattggc ttcccaatag gcatctatcg
     3121 tcccgacatc acgccaataa tagccatcac tctgcgcacc gacacagctg tcggtgaagc
     3181 gatgcgcctg caccttatat tttttaatga tgtaaggaat aatatcttgg ccgaaatcat
     3241 gattggagtt acgtgcatag gcgtctcgaa tcaattgctc atacaagaat ttggcattga
     3301 atacatagat ccccatgctg gcaaacgcca cctcaggatt attgggtgtt ggcttgggat
     3361 ttgccggttt ctcatcaaac gccaccacgc ggtcagtacc atctacttcc agcacaccaa
     3421 atcctttggc atcctctaat ggcacattaa tgcaggcaat cgtcatgtct gcattgctac
     3481 gcgcatgagt tgccagcatt ttgccgtagt ccattttgta aatatggtca cccgcaagca
     3541 ccagaatgta ttcaccacca ttctctcgca acaggtctat at
//
LOCUS       DATMSK010000105         6144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_243293,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000105 DATMSK010000000
VERSION     DATMSK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243293"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VNZ84_03610"
     CDS             <1..572
                     /locus_tag="VNZ84_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66072.1"
                     /translation="ASTIETGQAAQAAPDKRKKSKPAAAETAEMSGTMDPSFVDARSP
                     DSPMRSTTPKPLPAAKIASITPSVEPLLDDERMAHLLKLGGSEAFLYKLITEFVDTTD
                     ELMRSLGMHCLDLNFEQVHKLAHILAGESANMGLIPLSKHSRRLLGLSMEDAQTISSL
                     YQAVADCYEETRVQLHQHRSKLSSKRSNK"
     gene            complement(602..1576)
                     /locus_tag="VNZ84_03615"
     CDS             complement(602..1576)
                     /locus_tag="VNZ84_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWU66073.1"
                     /translation="MKPFEQGLVNYQQRLLSHDYGQVFCRFDAERQALWSYLNQPQRI
                     PCINHDLLMNLHEHHADIQRSGGYVQTDADSVLPIRYSVFASATPGYFNMGGDLQKMA
                     AAIRQQDRAQLQAYAKLSIDVVAQRAFRFQLDHVTKISLLQGEVLGAGIEAAMTSDVL
                     IAERHSVLCFPELFFNMIPGMGAYSFIARKAGMAVADKMILGCERFTAEECLEMGLID
                     IVVDEGEGEQAVYDFIHKNNKHAEGFLSAQKAKARINPLTYGELEDIVNVWVENALRL
                     TERDLKVMDRFYRAQSRLFPVSEESAVMNADTETGTQQADVQPRAVNQ"
     gene            complement(1781..4360)
                     /gene="acnB"
                     /locus_tag="VNZ84_03620"
     CDS             complement(1781..4360)
                     /gene="acnB"
                     /locus_tag="VNZ84_03620"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HWU66074.1"
                     /translation="MLQAYETHVKERAAQNLPPLPLNAEQVAALVELLKSPPAGQEAI
                     LLNLLENRIPAGVDQAAYVKAAFLADVANGKASSPLVSAEKAVALLGTMLGGYNVQAL
                     VALLDTPMAAHAVKALSTTILMFDAFHDVDVKMKAGNLHAKALMTAWANAEWFTSKPA
                     LAEEIKLVVFKLDGETNTDDLSPAQDAWSRPDIPLHAKAMLINRMADGLTTIEALKQK
                     GLPLAYVGDVVGTGSSRKSAINSVQWWMGDDIPYIPNKRTGGVVIGSKIAPIFFNTAE
                     DSGALPLQCDVSKLNTGDVIVIKPFEGKVLSESGELLSTFEMNPVTLPDEVRAGGRIP
                     LIIGRGLTANARKALGMGDTDTFIKSIPAKDTGKGYTLAQKMVGRACGLPEGVGVRPG
                     TYCEPKATTVGSQDTTGGMTRDELKELACLGFSADLVMQSFCHTAAYPKPVDIKLQHE
                     LPEFMSSRGGVSLRAGDGVIHSWLNRLILPDTVGTGGDSHTRFPIGISFPAGSGLVAF
                     AAATGAMPLDMPESVLVRFKGQMQPGITLRDLVNAIPYAAIQEGTLTVGKQGKKNVFN
                     GRVLEIEGLPDLKVEQAFELADASAERSANGCTVLLNKEPVIEFLNSNIVLMQNMIEN
                     GYADARTLERRIASMKAWLEKPELLQPDSDAEYAHVIEIDLNEIKEPLVACPNDPDDI
                     KPLSAVVGDKIDEVFIGSCMTNIGHYRAAGKVLEGAGGIPTRLWIAPPTRMDEAQLRA
                     EGVYSTFGVAGARTEVPGCSLCMGNQARVADKATVFSTSTRNFDNRMGKDARVYLGSA
                     ELAAVCAKLGRMPSVQEYLDTLGSKLTNTAEIYKYLNFNQMIQYAGSLENVKKIIPIK
                     ETV"
     gene            complement(4560..4883)
                     /locus_tag="VNZ84_03625"
     CDS             complement(4560..4883)
                     /locus_tag="VNZ84_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66075.1"
                     /translation="MIKMNNAVIGFAAGLVIGSSATVHAESALEYFTDSTGRYSGPSL
                     EEQVALMDTDHNGFADVFEVRAYLEKQHGKGYQKELLDKWESSAYGKSCSTPFAKELY
                     GDSTR"
     gene            complement(4975..>6144)
                     /locus_tag="VNZ84_03630"
     CDS             complement(4975..>6144)
                     /locus_tag="VNZ84_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HWU66076.1"
                     /translation="IVNDPAGSRKNTKDNTPAITHIEMVVGDKVEVQVAAKGGGSENK
                     SKLAMLNPNESIVDWVLEVVPEMGAGWCPPGMLGIGIGGSAEKAMLLAKESLMAPIDI
                     QEVQARGPQNRIEELRLELYEKVNQLGIGAQGLGGLTTVLDVKILDYPTHAASLPVAI
                     IPNCAATRHVHFTLDGSSVAELTPPSPNIYPDVHWAPSPQAKRVNLDTVTSAETQTWK
                     TGDTLLLTGKILTGRDAAHKRIQTMLAKGEPLPVDFTNKFIYYVGPVDAVRDEAVGPA
                     GPTTATRMDSYTDMMLNTGLLGSIGKAERGEEALAEIAKHKSVYLMAVGGAAYLVSKA
                     IKKSRVLAFADLGMEAIYEFEVVDMPVTVAVSADGESVHQTGPAQWKKTIALQAV"
BASE COUNT         1504 a         1709 c         1403 g         1528 t
ORIGIN      
        1 ttgcgagcac gattgaaact gggcaagctg cacaagcagc gcctgacaaa cgcaaaaaat
       61 ccaaacccgc ggccgcagaa acagcagaaa tgtctggcac gatggatcca agctttgtgg
      121 atgcgagatc accggattcg cccatgcggt caacaacacc caagccgttg cctgccgcta
      181 aaatcgccag cattactccc tcggttgaac ccttgctgga tgatgaacgc atggctcatt
      241 tgctcaaact gggcggtagt gaagccttcc tctacaagtt gattacagag tttgtggata
      301 ctaccgacga attaatgcgg tcactgggca tgcactgcct ggacctgaac tttgaacaag
      361 tgcataagct ggcacacata ctggcaggcg agtctgccaa tatgggcctg attccgttga
      421 gtaagcacag tcgtcgcttg ttgggcttga gtatggaaga tgcccagact atttccagcc
      481 tatatcaggc cgtcgcagat tgctatgaag agacgcgtgt gcagctacat caacaccgta
      541 gcaagctctc atccaaacgc tcaaacaaat agccgggcac cttgccattt tccccaccac
      601 cctattgatt cactgcgcgc ggctgcacat cagcctgttg cgtcccagtc tccgtatcgg
      661 cgttcatcac agcactttct tcacttacag ggaataatct ggattgggca cgatagaacc
      721 tatccatgac cttcaaatca cgctcagtca accgcagcgc gttttccacc cacacattaa
      781 caatatcttc caactcacca taagtcagtg ggttgattct ggctttcgct ttttgcgcgg
      841 ataaaaagcc ctcggcatgc ttgttattct tatgaataaa gtcatatacc gcctgctcac
      901 cttcaccttc atccacgacg atatcaatca ggcccatttc caagcactct tctgccgtaa
      961 aacgttcaca accgagaatc attttatcgg caaccgccat gcctgctttg cgcgcaataa
     1021 agctataagc acccatgcct ggaatcatgt taaaaaacaa ttcaggaaag caaagtaccg
     1081 agtgcctttc tgcgatcaac acatcactgg tcatagccgc ttcaatccca gcccccaaca
     1141 cctccccttg cagtaatgaa attttggtta catggtctaa ctggaaacgg aatgcgcgtt
     1201 gtgcaaccac atcaatactg agtttggcat aggcttgcag ctgtgcccta tcctgttggc
     1261 ggatggctgc cgccattttc tgcaaatccc cgcccatatt gaaatagccc ggagtggctg
     1321 aagcaaaaac agagtaacgg ataggcaata cgctatcagc gtccgtttgt acatagccgc
     1381 cactacgctg aatgtccgca tgatgctcat gcagattcat cagtaaatca tgattgatgc
     1441 agggaatacg ctgaggttga ttcaaataac tccacagcgc ttgacgttca gcatcaaaac
     1501 ggcaaaagac ctgaccgtaa tcatgtgaca gtaaacgttg ttgataatta accaggcctt
     1561 gttcgaacgg tttcatgagt ttctccttgg acattgaaaa tctgttaagt gaatcagggg
     1621 tgacatattg agatcaattg attgagctcg caaacacatc ataatcaaaa caggagctta
     1681 ccgtcagggg ccttttcagg ttttacagtt gcaaggatat ttcccgccgt aaaaacaaca
     1741 aagggagctt acgctccctt tgatcattgc tgcagtctgg ctatacagtt tctttaattg
     1801 gaatgatttt cttcacattt tccagcgagc ccgcatattg gatcatctgg ttaaagttga
     1861 gatatttgta aatctctgcc gtgttggtca gcttgctgcc caaagtatcc aggtactctt
     1921 gcacgctagg catgcgcccc agtttggcac ataccgcagc caattcggca gatcccaggt
     1981 acacgcgtgc atctttaccc atacggttgt caaagttacg tgtcgaggta gagaatactg
     2041 tcgccttgtc agccacgcgt gcctgattac ccatacacaa ggaacagccc ggcacttctg
     2101 tacgcgcacc agccacgcca aaggtggagt acaccccttc agcacgcaat tgcgcttcat
     2161 ccatacgggt cggcggcgca atccacaagc gggtcgggat accccctgca ccttccagca
     2221 ctttgcctgc tgcacggtaa tggccgatgt tggtcatgca gctaccaata aacacttcgt
     2281 caatcttgtc acctaccacg gcagacaagg gcttgatatc gtctggatca ttcgggcaag
     2341 ccaccagtgg ttctttgatc tcattgaggt cgatttcaat cacatgggca tattcagcat
     2401 cactgtcagg ctgtaacagc tccggttttt ccaaccaggc tttcatgctg gcaatacggc
     2461 gttccagcgt gcgtgcatcg gcatagccgt tttcgatcat gttctgcatg agcacgatat
     2521 tggagttgag gaactcaatc accggctctt tattcaacag cacagtacaa ccattggcag
     2581 aacgctcagc cgaagcatca gccagctcaa atgcctgctc caccttcagg tctggtaaac
     2641 cttcaatttc cagtacgcgg ccgttaaaca cgtttttctt gccttgttta ccaacagtca
     2701 gtgtgccttc ctggatcgct gcgtaaggaa tcgcatttac caggtcacgt aaggtgatac
     2761 caggttgcat ttggcctttg aaacggacca gcacagattc tggcatatcc agcggcatgg
     2821 ccccggtggc cgccgcaaaa gcgactaatc cagagccggc cgggaaactg atcccaatcg
     2881 ggaagcgggt atgtgaatca ccacctgtac ctacggtatc cggcagaatc aagcggttga
     2941 gccaggagtg gatcacacca tcccccgcgc gcaggctgac accgccacgg ctggacatga
     3001 actctggcag ctcatgttgt aatttgatat cgacaggctt cggataagcc gcagtatggc
     3061 agaagctttg cattaccagg tcagcactaa agcccaggca cgccagctct ttcagctcat
     3121 cgcgtgtcat accacccgtg gtatcttgtg aacccaccgt agttgcttta ggttcgcaat
     3181 aagtcccagg acgcacaccc acgccttcgg gcaaaccaca ggcacgaccc accatttttt
     3241 gtgccagggt ataacctttg cccgtatctt tagctggaat ggatttaata aaagtatccg
     3301 tgtcacccat acccagtgct ttacgcgcgt ttgcagtcag gcctcggcca atgattaatg
     3361 ggatacggcc accagcgcgg acttcatccg gcaaggtgac tggattcatt tcaaaagtgg
     3421 ataacagctc gccagattca gacagcactt tgccttcaaa tggcttgatc acaatcacat
     3481 caccggtatt gagcttggac acatcacact gaagtggcaa cgccccggaa tcttctgccg
     3541 tgttaaagaa aattggcgca attttgctac cgatgaccac gccaccggtg cgcttgttgg
     3601 ggatataggg gatatcatcc cccatccacc attgcacaga gttaatcgca gatttacgcg
     3661 aagaaccggt acccaccacg tcacccacat aagccagcgg caggcctttt tgttttaagg
     3721 cttcgatagt ggtcaatcca tccgccatgc ggttaatcag catggctttt gcatgcagag
     3781 gaatgtccgg acggctccag gcatcttgcg ccgggctcag gtcatcggta ttggtttcac
     3841 catccagctt aaacaccacc agtttgattt cttcagccaa cgcaggcttg cttgtaaacc
     3901 attcggcatt ggcccaagcg gtcatcaacg ctttggcatg caggttacct gccttcattt
     3961 tgacatccac atcatggaaa gcgtcaaaca tcaggatggt ggtagacaag gccttaactg
     4021 cgtgtgcggc cataggggta tccaacaaag ccaccaatgc ctgcacgttg tagcctccca
     4081 gcatggtgcc taacaacgct acggcttttt cagcactcac cagtggtgag ctggctttgc
     4141 cattcgcaac atccgccaaa aaagcagcct tgacataagc ggcctggtca acacccgcag
     4201 gaatacggtt ctccagcaga tttaacagga ttgcttcttg tccggccgga ggagatttta
     4261 ataactcaac caatgcggcg acttgttccg cattgagtgg caagggaggc aggttttgcg
     4321 cagcgcgctc ttttacgtgg gtttcgtatg cttgtaacat gatgtaggct ctatcaataa
     4381 aacaaagtcg ggggatgggc cagcacttat ctgtaacgac atgaccagag gctgaccgcg
     4441 aggatttctt cttagttgat tataatccag tccataagcc agaatgttaa ctgcttgtaa
     4501 cattccggca attgatgcaa atgacagccg ccaacagcaa caaaacaaca ctactgcgat
     4561 cagcgtgtac tgtctccata taactctttg gcaaatgggg tgctacaact ttttccgtag
     4621 gcggaagact cccatttatc cagtaactct ttttgatacc ccttgccgtg ctgcttctct
     4681 agataggcac gcacctcaaa cacatcggca aaaccgttat gatctgtgtc catcagggcc
     4741 acctgctctt ccagcgaagg tccactgtag cggccagtac tatcggtgaa atattcgagc
     4801 gcagactcgg catgcacagt cgcactcgag ccgatgacca agcccgctgc gaacccaatt
     4861 accgcattat tcatttttat catactcact ccattgtgac gcttagtagt atgacgtatc
     4921 tttcaccaat tcgtgcccgg cctctgtgat tgcactcaca ttatcggcag tcatttatac
     4981 cgcttgcaac gcgatcgttt ttttccattg tgccgggccg gtctggtgta cggactctcc
     5041 atccgcactc actgctaccg tcactggcat atccaccact tcaaattcat aaatggcttc
     5101 catccctaag tcagcaaaag ccaatacacg ggatttttta atcgccttgc tcaccaggta
     5161 agcggccccg ccgacggcca tcaggtatac cgatttgtgt ttggcaattt cagccagtgc
     5221 ttcttcgcca cgctcagctt taccaatcga acctagcaag ccggtattca gcatcatatc
     5281 ggtatagcta tccatgcggg ttgccgtcgt tgggcctgca ggtccaacag cttcatctcg
     5341 cacagcatcg accggaccga cgtagtaaat aaacttattc gtgaaatcca caggtaaggg
     5401 ctcgcccttg gccagcatgg tctggatgcg tttatgtgcg gcatcacgcc ccgtcaggat
     5461 tttgccagtg agcaacaagg tatcaccggt tttccaggtt tgcgtctctg ccgatgtcac
     5521 cgtgtccaga tttacgcgtt tggcttgtgg gcttggtgcc cagtgcacat ccgggtaaat
     5581 attcggacta ggcggtgtca attcagctac actggaacca tccagggtaa agtgcacatg
     5641 tcgcgtcgca gcacaattag ggataatggc taccggtaaa ctggcggcat gggttggata
     5701 atccagaatc ttcacgtcga gcactgtggt caggccaccc agaccttgcg cgcctatgcc
     5761 tagctgattg accttttcat acagttctaa ccgcaactct tcaatgcggt tttgtggacc
     5821 acgcgcttgt acttcctgaa tatcaattgg tgccatcaag gactctttgg ccagcaacat
     5881 ggctttttca gcactgccgc ctatgcctat acccagcatg ccgggcgggc accagccagc
     5941 acccatttct ggcaccactt ccagcaccca gtcgacaata ctttcatttg gattcagcat
     6001 cgccagcttg gacttattct cagagccgcc gcctttggca gcaacttgta cctcaacctt
     6061 atcacccacc accatttcaa tatgcgtaat cgcgggtgta ttgtctttag tgtttttacg
     6121 cgacccggcc gggtcattca caat
//
LOCUS       DATMSK010000106        10485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_31686,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000106 DATMSK010000000
VERSION     DATMSK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31686"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VNZ84_03635"
     CDS             <1..364
                     /locus_tag="VNZ84_03635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HWU66077.1"
                     /translation="QKEIADGLAAAQEGRSALEVAAKKSDATLNEAKQKASEIIGQAE
                     KRAAQIIDEAKANAKAEGDRIIAGAKAEIDQEVNRAKEGLRAQVSTLAVAGAEKILRK
                     EIDAKAHSEMLTKLAAEL"
     gene            374..910
                     /locus_tag="VNZ84_03640"
     CDS             374..910
                     /locus_tag="VNZ84_03640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HWU66078.1"
                     /translation="MAELSTIARPYAVAAYKLGKEKKALAKWSEMLAFAAAVATDAQM
                     KTLIDNPKLNAADIEAAFLKVSGDNLNDHGQNLIKTLVEYGRLSLLPAIAEAFEALKA
                     QDEGTLDAQIIAAAKITATQTKDLVKRLEAKFGKKIEASVSVDPEIIGGIKIIVGDTV
                     IDASVQGQLQNLAYTLTA"
     gene            927..2468
                     /gene="atpA"
                     /locus_tag="VNZ84_03645"
     CDS             927..2468
                     /gene="atpA"
                     /locus_tag="VNZ84_03645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWU66079.1"
                     /translation="MQLSTSEISELIKSRLENFSTTAEARTQGTVISVTDGIVRIHGL
                     SNVMAGEMIEFPGNTFGLALNLERDSVGAVVLGDYEHITEGDVCKCTGRILEVPVGPE
                     LIGRVVNSLGQPIDGKGPVKTKLTDKIEKIAPGVIARKSVSQPVQTGLKSIDSMVPVG
                     RGQRELIIGDRQTGKTAVAVDAIINQKGQNMTCIYVAIGQKASTIANVVRKLEEHGAM
                     AYTIVVAASASDSAALQFLAPYAGCTMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYIHSRLLERAARVNEEYVEKFTEGKVKGKTGSLTALPVIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGVRPAINAGISVSRVGGAAQTKIIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEATRKQLDRGRMFTELMKQAQYAPLSVATMGVSLFA
                     ANKGYFDDVPTNKVLAFENALHGFLNSKYKNIMDAIESSKDLSGDNEKALEAAIQDFK
                     ATNAY"
     gene            2498..3367
                     /gene="atpG"
                     /locus_tag="VNZ84_03650"
     CDS             2498..3367
                     /gene="atpG"
                     /locus_tag="VNZ84_03650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HWU66080.1"
                     /translation="MAGSKEIRTKIKSVENTRKITKAMEMVAASKMRKAQDRMRASRP
                     YAEKIRNVAAHLSFAQSEYRHPFLIKRDVVKNVGLIVVSSDKGLCGGLNTNVLRLSLN
                     QMKAWEAEGKGISVSAIGNKGYGFMNRIGAQVKSHITGLGDTPHLEKLIGSIKVMLDA
                     YIAGEIDQLYICYTKFINTMKQEPTMQQLLPLSGEQLGSPNGHWDYIYEPEAKPVVDE
                     LMTRYIESLIYNAVAENMASEQSARMVAMKSASDNAKSVIGELKLVYNKARQAAITKE
                     ISEIVGGAAAVSS"
     gene            3432..4868
                     /gene="atpD"
                     /locus_tag="VNZ84_03655"
     CDS             3432..4868
                     /gene="atpD"
                     /locus_tag="VNZ84_03655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWU66081.1"
                     /translation="MAKATQAVTGKVVQCIGAVVDVEFPRDQMPKVFDALIIDDKNSN
                     AEAGLTLEVQQQLGDGVVRTIAMGSSDGLARGTAFKNTGGAISVPVGEGTLGRIMDVL
                     GRPIDEVGPIDSKEFRSIHQKAPAFEELSPSVDLLETGIKVIDLVCPFAKGGKVGLFG
                     GAGVGKTVNMLELINNIAKQHSGLSVFAGVGERTREGNDFYHEMAEAGVIKLDNMKES
                     KVAMVFGQMNEPPGNRLRVALSGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALL
                     GRMPSAVGYQPTLADEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFQHLD
                     STVVLSRDIASLGIYPAVDPLDSTSRQLDPQIVGEEHYSVARSVQSTLQRYKELRDII
                     AILGMDELSPEDKLSVGRARKIQRFLSQPFHVAEVFTGAPGKYVPLKETIKGFKAIVS
                     GEYDHLPEQAFYMVGGIEEAVEKAKTLN"
     gene            4936..5361
                     /locus_tag="VNZ84_03660"
     CDS             4936..5361
                     /locus_tag="VNZ84_03660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWU66082.1"
                     /translation="MANTVHIDVVSAEESIFSGEAEFVVAPAGGGEVGIYPNHAPMIT
                     TIKPGALRIKETEGKDETVIFISGGMLEVQPGIITVLADTAIRGQDLDEAKAIAAKEA
                     AEEALKNRSAEIDYAVAQAELAEAVAQIQAIQKLRKSVH"
     gene            5440..6810
                     /gene="glmU"
                     /locus_tag="VNZ84_03665"
     CDS             5440..6810
                     /gene="glmU"
                     /locus_tag="VNZ84_03665"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWU66083.1"
                     /translation="MTDLNIVILAAGKGTRMRSDLPKVLHAVGGKPILHHVIAAAREL
                     NPQSITIVYGFGGAQVQQQTPGDDLQWVLQAEQLGTGHAVQQALPFLPDQGKTLILLG
                     DVPLVDVTACRDMLVQADQHLVVQTFIKDDPTGYGRIVRNDQNEVVAIVEHKDASDAQ
                     RAILEVNTGIMAMPNVQLKSWLGKISNQNAQQEYYLTDIIALSVLEGRAVLPHVVADA
                     WSVTGINSKLDLQEIEREYQNRQASALMANGVTLLDAKRIDIRGTLNCGRDVMVDVNC
                     VFEGQVTLEDGVEIGPHCVIKNAVIAKGTKLAAFTHIDDVTIGQDSKIGPFARLRPGT
                     RLADKTHVGNFVEIKNATVGLGSKVNHLSYVGDSIVGSLVNIGAGTITCNYDGANKHK
                     TIIEDEVFVGSDTQLVAPVTVGRGATIAAGSTITKDVPADSLTLCRAREQRSIQGWKR
                     PQKVKK"
     gene            6815..8650
                     /gene="glmS"
                     /locus_tag="VNZ84_03670"
     CDS             6815..8650
                     /gene="glmS"
                     /locus_tag="VNZ84_03670"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWU66084.1"
                     /translation="MCGIVGAVSNRNIVPLLIEGLSRLEYRGYDSAGIAVLNQDIERV
                     RAVGRVSELQQKAQGMHSQIGIGHTRWATHGGVTEQNAHPHVSPSQDMAQIAVVHNGI
                     IENHDEQRDRLKALGYAFYSQTDTEVIAHLVHYYYQQSQDLFIAVRQAITELTGAYAI
                     SVIALDNPKQMVVARLGCPLLVGLGDGENFVASDVSAVLSATRKVIYLEDGDVATLQT
                     DAVVIVDKQGEIAQRKVHMSDVSLASLELGPYSHFMQKEIHEQPRAISDTIEALIDDG
                     AFRSELFGQEAGEIFAQVNSVLILAAGTSYYAGLTAKYWLESIAKIPTSVEIASEYRY
                     RESVPNPKQLIVTISQSGETLDTMEALKHAQKLGHTYSLAICNVQESAIPRASSLIFY
                     TRAGAEIGVASTKAFTTQLIALFVLAVTLGKARDLLSAAQEQQYLADLRQLAGSVQIA
                     LNLEPQIREWAHRFGDKDHALFLGRGVHFPIAMEGALKLKEISYIHAEAYPAGELKHG
                     PLALVDSNMPVVVIAPNDALLEKVKSNMQEVKARGGELFVFADADSHFHASEGVHVIR
                     TPRHVGVLSPILHAVPVQLLAYHAALLKGTDVDKPRNLAKSVTVE"
     gene            8754..9974
                     /locus_tag="VNZ84_03675"
     CDS             8754..9974
                     /locus_tag="VNZ84_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU66085.1"
                     /translation="MSTSQHSIIGRQPIVNSQQEIIGYEFFFRNDSDGSHAAFAEDIQ
                     TCAKILSTTIDEMQENWLLGQQLAFINVDHVMLSSEFLELMPAEKTVLEMVGSLEVTP
                     AILERCRALKQNKFKLALDNPQKNPQLDALIPYADYIKLDMRDVDLAQSTTWFKKYQA
                     NNCKLIAEKVEQNAQFSSLLEVGYQYFQGYFYAKPENLSSKVINPSFDGVLHLLNLVS
                     QEADNKTIENGFKRDTTLSYKLLRYINSVGFGLSCEVQSINHALTILGRNQLYRWLTL
                     LMVTAGNNSSSPALMKTSITRGRLTELLGEPFFDKRDRDNLFVVGVFSLLDVMLKVPM
                     DQVLEKLQLPEPIVDALTKREGIFGPFLKLTEACEDANNAEILQLAALLQLNPEKVTQ
                     CHMEALAWTEALGI"
     gene            10088..>10485
                     /locus_tag="VNZ84_03680"
     CDS             10088..>10485
                     /locus_tag="VNZ84_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HWU66086.1"
                     /translation="MRTYDLTPFYRTAIGFDRLAKLLNESQRNENDISYPPYNIELVD
                     ENAYKIVMAVAGFQQDELEIETEHQALRVTGRKAKKEQETTFLHRGIATRDFEHKFQL
                     ADHVKVTGANLENGLLTIALLREVPEALKPR"
BASE COUNT         2921 a         2354 c         2648 g         2562 t
ORIGIN      
        1 ccaaaaagaa atcgccgatg gtttggctgc tgcacaagaa ggcagaagtg ccttggaagt
       61 agcggctaaa aaatctgacg caacactgaa tgaagcaaaa caaaaagcaa gtgagatcat
      121 tgggcaagct gaaaaacgcg ctgcacaaat catcgatgaa gccaaggcga atgccaaagc
      181 tgaaggtgat cgcattattg ctggtgccaa agctgagatc gaccaggaag tgaatcgcgc
      241 taaagaaggt ttgcgcgctc aagtatctac attggcagtt gccggtgcag agaaaatcct
      301 gcgtaaagaa atcgacgcca aagcacatag cgagatgctc actaaacttg ctgcggaact
      361 ataaggataa aacatggctg aactctcaac cattgcaaga ccttatgccg tcgccgccta
      421 caagctgggc aaagagaaaa aggctctggc aaaatggtct gaaatgctgg cgtttgcagc
      481 agctgttgcg acagatgcac aaatgaaaac tttgattgat aacccgaaac tgaatgcagc
      541 agatatcgaa gcggcattcc ttaaagtgag cggcgacaat cttaacgatc atgggcaaaa
      601 tttaatcaag actttggttg aatacggccg tttatcctta ttgcctgcca ttgctgaagc
      661 ttttgaggct ttgaaagcgc aagatgaagg caccttggac gcacaaatca tcgcggctgc
      721 caaaatcacg gctacgcaaa ctaaagattt ggtaaaacgt ctggaagcca agttcggcaa
      781 gaaaattgaa gcaagcgttt ccgtagatcc ggaaatcatc ggcggcatca aaattattgt
      841 tggcgacacc gtcatcgacg cgtctgtcca aggtcagtta caaaatttag cctatacgct
      901 aacagcataa ggcataggag atttaaatgc agttatcaac atcagagatt agcgagctca
      961 ttaagagccg tttggaaaac ttctctacta ccgctgaagc acgtactcaa ggtaccgtga
     1021 tttctgtgac agacggcatt gtgcgtatcc acggcctgtc taacgtcatg gctggtgaga
     1081 tgattgagtt tccaggcaac acattcggtt tggcactgaa cctggagcgt gactccgttg
     1141 gtgctgtggt gctgggtgat tacgagcaca tcaccgaagg tgacgtgtgt aaatgtaccg
     1201 gtcgtattct ggaagtacca gtaggtccgg aactgattgg ccgtgtggtg aactcactgg
     1261 gtcagcctat cgatggtaaa ggtccagtta aaaccaaact gaccgacaag attgaaaaaa
     1321 ttgcgccagg cgtgattgca cgtaaatctg tgtcacaacc agtgcaaact ggcctgaagt
     1381 ctatcgactc catggtacca gttggccgtg gccaacgtga attgatcatt ggtgaccgtc
     1441 agactggtaa aacagccgtt gccgttgatg cgatcatcaa tcaaaaaggt cagaacatga
     1501 cctgtatcta cgttgcgatt ggtcagaaag cttctaccat tgctaacgtg gtacgtaaac
     1561 tggaagagca cggcgcaatg gcatatacca tcgtggttgc tgcttcagct tctgactccg
     1621 cagccctgca attcctggca ccttatgccg gttgcaccat gggtgaatac ttccgtgacc
     1681 gcggtgaaga tgcattgatc gtttacgatg acttgtccaa acaagcggtt gcttaccgtc
     1741 aaatctccct gttgctgcgc cgcccaccag gccgtgaagc ttatccaggt gacgtgttct
     1801 acatccactc acgtttgtta gagcgtgctg ctcgtgtgaa cgaagagtat gttgagaaat
     1861 tcactgaggg caaagttaaa ggcaaaacag gttcattgac tgccttgcca gtgattgaaa
     1921 cacaagctgg tgacgtgtct gcattcgttc caaccaacgt aatttctatt accgatggtc
     1981 agatcttcct ggaaacagac ttgttcaacg caggtgtacg tccagcgatt aacgccggta
     2041 tttccgtatc acgtgttggt ggtgctgcac aaaccaagat catcaagaaa ttgggcggtg
     2101 gtgtacgtct ggctttggcg caatatcgtg aattggctgc atttgcacag ttcgcttccg
     2161 atctggacga agcaacccgt aaacagctgg accggggtcg tatgtttact gaactgatga
     2221 agcaggcaca atatgcacct ctgagcgtag caaccatggg cgtgagcttg tttgcagcga
     2281 acaaaggtta ctttgacgac gtaccaacca acaaagtatt ggcatttgaa aatgcattgc
     2341 atggtttctt gaactccaag tacaagaaca tcatggacgc aatcgaatcc agtaaagatc
     2401 tgagtggcga taacgagaaa gcattggaag ctgcgattca agatttcaaa gcaaccaacg
     2461 cttattaatc tctgacactt cgacttagga acctaatatg gcaggtagta aagagattag
     2521 aaccaagatc aagagtgtag aaaatacacg caagatcacc aaggcgatgg aaatggtagc
     2581 cgcttctaaa atgcgtaaag cgcaggacag aatgcgtgct tcccgtccgt atgcggaaaa
     2641 aatccgcaac gtggcggctc acctctcctt tgcacaatcc gagtatcgtc atccgtttct
     2701 gatcaagcgt gatgtggtga aaaacgttgg cctgattgtg gtcagttctg acaaaggcct
     2761 ttgcggtggg ttgaatacca acgtattgcg tttgtccctg aaccagatga aagcctggga
     2821 agctgaaggc aaaggcatca gcgtcagcgc gatcggtaat aaaggctatg gctttatgaa
     2881 ccgtatcggc gcacaggtga aatctcacat cacaggtctg ggcgatacac cgcatctgga
     2941 aaaactgatc ggtagcatca aagtgatgct ggatgcgtac attgccggtg aaattgatca
     3001 gctttacatc tgctatacca agttcatcaa taccatgaag caagagccta ccatgcagca
     3061 actgctgcca ttgtcaggtg agcaactggg cagccctaac ggccattggg attacatcta
     3121 tgagccagaa gctaaaccag tggtagatga gttaatgacg cgttatatcg agtcactgat
     3181 ttacaacgca gtggccgaaa acatggcgtc agaacaatct gcacgtatgg tggcgatgaa
     3241 gtctgcttcc gacaacgcga agagcgtgat cggcgaactg aaactggtat acaacaaagc
     3301 ccgtcaggct gcgattacca aagaaatttc agaaatcgtc ggcggtgcag cagcggtttc
     3361 atcctaaggc atgctttaaa gcatagtgaa agaattcaat tagatctgac tcatacagtt
     3421 aggaaaagaa aatggcaaag gcaactcaag cagtaacagg taaagtagtg cagtgtattg
     3481 gcgctgtggt tgacgtggaa ttcccacgcg atcaaatgcc taaagtattc gatgcattga
     3541 tcattgatga caaaaactca aatgcagaag ccggtttgac actggaagtg caacagcagc
     3601 tgggtgacgg cgtggtgcgt accattgcca tgggttcctc agacggtctg gcacgcggta
     3661 ctgcatttaa aaacactggt ggcgcgattt ccgtaccagt aggtgaaggc acattgggcc
     3721 gtattatgga cgtattgggt cgcccaatcg acgaagtcgg tcctattgat tcaaaagaat
     3781 tccgttctat ccaccagaaa gcaccagcgt tcgaagaact gtctccttca gttgacctgt
     3841 tggaaacagg catcaaggtg attgacttgg tttgcccatt tgctaaaggt ggtaaagtgg
     3901 gtctgttcgg cggtgcgggt gtgggtaaga ccgttaacat gctggaactg attaacaaca
     3961 ttgctaaaca gcactcaggt ttgtccgtat ttgctggtgt gggtgagcgt actcgtgaag
     4021 ggaacgactt ctaccatgaa atggcagaag ctggtgttat caaactggac aacatgaaag
     4081 agtccaaagt agcgatggtg ttcggtcaga tgaacgaacc tccaggcaac cgtctgcgtg
     4141 tagcgttgtc tggtttgacc atggcggaaa aattccgtga cgaaggccgt gacgtgttgt
     4201 tcttcgtgga taacatctac cgttacacac tggctggtac tgaagtatca gcgctgctgg
     4261 gccgtatgcc ttcagcggta ggttaccaac ctaccctggc tgatgaaatg ggccgtttgc
     4321 aagagcgtat tacttctact aaagtgggtt ccattacttc tatccaagcg gtttacgtac
     4381 cagcggatga cttgactgac ccatcaccag caacaacctt ccagcacttg gactcaacag
     4441 ttgtgttgtc acgtgacatc gcttccctgg gtatctaccc agcggtggat ccactggatt
     4501 caacatcacg tcagctggat ccacaaatcg tgggtgaaga gcactactct gtagcacgtt
     4561 cagtacaatc aacactgcaa cgctacaaag aattgcgcga cattattgcg attctgggta
     4621 tggacgaatt gtctccagaa gacaaactgt ctgtaggccg tgcacgtaaa atccaacgtt
     4681 tcctgtcaca acctttccac gtggctgaag tatttactgg cgcgccaggt aaatacgtac
     4741 cactgaaaga aacgatcaaa ggctttaaag caatcgtttc cggtgaatac gatcacttgc
     4801 cagaacaagc gttctacatg gttggtggta tcgaagaagc tgttgagaaa gctaaaacac
     4861 tgaactaatg tatgtgatac aagcaggtgg tcatcatctg cttgcttctt tcagtgatat
     4921 agaaaagaga acattatggc aaatacagtg catatcgatg tagtcagcgc cgaagaatcc
     4981 atcttctccg gtgaagctga attcgttgtc gcaccagcgg gtggcggtga agtgggtatt
     5041 taccctaacc acgctccgat gattacgacc atcaagccag gtgctttgcg tatcaaagaa
     5101 actgaaggta aagatgaaac tgtgattttt atttccggcg gcatgctgga agtacagcct
     5161 ggcatcatta ccgtgttggc tgataccgcg attcgtggcc aggatctgga tgaggcgaaa
     5221 gcgattgccg caaaagaagc cgcagaagaa gcgttgaaaa accgctctgc agaaatcgat
     5281 tatgcagtcg cgcaagctga gctggctgaa gccgtggcac aaattcaggc aatccaaaaa
     5341 ttgcgtaaat ctgtacatta aacattgtgt actagaggca aataacaaaa caaggtggct
     5401 ttcgagccgc cttttttgtt tctgagataa aataacagca tgaccgattt gaatattgtg
     5461 atactcgctg caggtaaagg cacccgcatg cgatctgacc tgccaaaagt gctgcatgct
     5521 gtcggcggca agccgatttt gcatcatgtc attgctgcag cgagagaatt gaatccgcaa
     5581 tccatcacaa tcgtgtatgg cttcggtggt gcacaagtac agcaacagac gcctggcgat
     5641 gatttgcaat gggtattaca ggcagaacag ctaggtactg gccatgcagt acaacaagcc
     5701 ctcccctttt tgcctgatca gggcaagaca ctcattctat taggggatgt gccattggtg
     5761 gatgtgactg cttgccgcga tatgctggta caggcggacc aacatctggt ggtgcaaact
     5821 tttatcaagg atgatccgac agggtatggt cgtatcgtgc gcaatgatca gaatgaagtg
     5881 gttgccatcg ttgagcataa agatgccagt gacgcacaac gtgcaatact agaagtgaat
     5941 accggcatca tggctatgcc caatgtgcag cttaaatcat ggctgggcaa aatcagcaac
     6001 cagaatgcac aacaagaata ctatttgacc gatattattg ccctatcagt ccttgaaggg
     6061 cgtgccgtgt tgccgcatgt tgtggctgat gcctggtcag tgacaggcat taactccaaa
     6121 cttgatttgc aggaaattga acgcgagtat caaaatcgac aagcctcagc cttaatggca
     6181 aatggtgtga cattattgga tgctaaacgt atcgatattc gcggtacatt gaactgtggc
     6241 cgtgatgtca tggtagatgt gaattgcgtg tttgaaggcc aagtcacgct tgaagatggc
     6301 gtcgaaatcg ggccgcattg cgtgattaaa aatgcggtga ttgccaaagg tacaaaactg
     6361 gcggcattca cccatattga tgatgtcacc ataggccagg atagtaaaat cggcccattt
     6421 gcacggttgc gtccagggac acgattagcc gataaaaccc acgtcggaaa cttcgttgaa
     6481 atcaagaatg ctaccgtagg tctaggcagc aaagtcaacc atttgagcta tgtgggtgac
     6541 tccattgtgg gtagccttgt aaacattggg gctggaacga tcacttgtaa ttatgatggc
     6601 gcaaacaaac ataaaaccat tatcgaagat gaggtgtttg tgggctctga tacacaattg
     6661 gtggcacccg tgactgtggg gcgtggtgca acgattgcag ctggctcaac gattaccaaa
     6721 gatgtgcctg cagactcatt aacgttatgc cgtgcacgtg agcaacggtc aattcaagga
     6781 tggaaacgtc cacaaaaggt gaaaaaataa gtatatgtgc ggtattgtcg gtgctgtttc
     6841 aaacaggaat atcgttccat tattgattga gggcttaagt cgactcgagt atcgcgggta
     6901 tgactccgcc gggatcgcgg ttcttaatca ggatattgag cgtgtacgtg cagtaggccg
     6961 tgtctcagag ttacaacaaa aagcacaagg tatgcacagc cagattggca tcggccatac
     7021 ccgttgggcc acccatggcg gcgtcactga acaaaacgcg catcctcatg tatcacccag
     7081 ccaagacatg gcgcagattg ctgtggtgca taacggaatt atcgaaaatc atgatgaaca
     7141 acgtgaccgc ttaaaagcgt tgggatatgc attttattcg caaactgata ccgaagtcat
     7201 cgcacatctg gtgcattact actatcagca aagccaggat ctgtttattg cggttaggca
     7261 ggccattacc gagctgacag gtgcatatgc catcagcgtg attgccctgg ataatcccaa
     7321 gcagatggtc gttgcacgac tgggctgccc attgctggtt ggtttgggcg acggtgaaaa
     7381 ttttgtggca tctgatgtgt ctgcagtgct atcagcgacg cgtaaggtga tttacctgga
     7441 agatggcgat gtcgcgactc tgcaaaccga tgcagtggtg attgtcgaca aacaaggtga
     7501 aattgcgcag cgcaaagtcc atatgagcga tgtgtctctt gcgtcacttg agcttggtcc
     7561 ttacagccac tttatgcaaa aggaaatcca tgaacagcca cgtgccatca gtgacacgat
     7621 tgaggccttg attgacgatg gcgcattccg aagtgaattg tttggtcagg aagcgggcga
     7681 gattttcgca caggttaaca gtgtattaat cttggcagct ggcacgagtt attacgcggg
     7741 gctcactgcc aaatactggc tggaaagcat tgctaaaatc ccaacttcgg tcgagattgc
     7801 cagcgaatac cgctaccgtg agtccgtgcc taatcccaag cagctcatcg tcactatttc
     7861 acaatctggt gaaacactgg ataccatgga agcgctcaag catgcacaaa agcttgggca
     7921 tacttacagt ttggctattt gcaatgtgca ggaaagtgcc attccacgcg cttcatcact
     7981 cattttctac acacgcgccg gcgccgagat cggtgtggcg agtaccaagg catttacaac
     8041 acaactgatc gctttattcg tgctggcagt gaccctgggc aaagcacgtg atttactgag
     8101 cgcagcacaa gaacaacaat atctggccga tttgcgccaa ctggccggga gtgtgcagat
     8161 tgcattgaac ctggagccac aaatcaggga atgggcacac cgatttggtg ataaggacca
     8221 tgcactgttc cttggacgtg gcgtacattt cccgattgca atggaaggag cgctcaagct
     8281 caaggagatt tcctatatac atgcggaagc ctatcctgcc ggtgagctta aacatggccc
     8341 actggccttg gtggacagca atatgcccgt cgtcgtcatt gcaccgaatg acgcgctgct
     8401 ggaaaaagta aaatccaata tgcaggaagt gaaggcccgg ggtggcgagt tattcgtctt
     8461 tgccgatgca gacagtcact ttcatgcttc ggaaggtgtg catgtcattc gtacgccacg
     8521 ccatgtaggg gtattgtcac caatcctgca tgcggtacca gtacagttgc tggcttacca
     8581 tgcagctttg ctgaaaggta cggatgtgga taaacctcgc aacttggcca aaagtgtgac
     8641 tgttgaataa gcaatagatc tctaaaaaag cctcgggatt tgaggctttt ttacaataaa
     8701 aaatcaatca atttccctta tactgttacc cggagttcat caggaaggcc atcatgtcca
     8761 cgtcacaaca cagcattatt ggtcgtcagc cgattgttaa cagtcaacaa gagattattg
     8821 gctacgaatt tttcttcaga aatgatagcg atggcagcca tgccgcattc gcagaagata
     8881 tccaaacctg tgccaaaatt ctttccacca ccattgatga aatgcaggaa aactggttgc
     8941 ttgggcaaca gctggcattt atcaatgtcg atcatgtgat gctcagtagt gagtttctgg
     9001 agttgatgcc tgcagaaaaa acggtgcttg agatggtggg tagcttagag gtcactcccg
     9061 ccatactcga acgttgccgt gcgcttaaac aaaacaaatt caaactggca ctggataatc
     9121 cgcaaaaaaa tccacagctg gatgcactga taccttatgc cgattacatc aagctcgata
     9181 tgcgcgatgt tgacctggct cagtctacga cctggttcaa aaaataccaa gcaaataatt
     9241 gcaaactgat cgctgagaag gttgaacaga atgctcagtt cagcagcctg ctggaggttg
     9301 gctaccaata cttccaaggc tatttctatg ccaagccgga aaacctgagt agcaaagtga
     9361 ttaaccccag ttttgacgga gtgttgcatt tgctcaatct ggttagccag gaggcagata
     9421 ataaaaccat agagaatggt ttcaaacgcg acaccacttt aagctacaag ttattacgtt
     9481 atattaattc ggtaggcttt ggcttgtctt gcgaagtgca gtcaattaat catgccttga
     9541 cgatactggg ccgcaatcag ctctaccgct ggttgacctt gcttatggta accgcaggca
     9601 ataacagcag ctcccctgcc ctgatgaaaa cttccatcac gcgtggccgt ttgactgaac
     9661 tactgggtga gccatttttt gacaaacgtg accgtgataa cctgtttgtc gttggtgtgt
     9721 tttcattact ggatgtgatg ttaaaagtcc ccatggacca ggtcttggaa aagctgcaat
     9781 tgccagaacc aattgtcgat gcgttgacca aacgtgaagg catttttggg ccgtttctca
     9841 agctcactga ggcttgtgaa gatgctaaca atgctgaaat cctccaattg gccgccttgc
     9901 ttcaactgaa tccggaaaaa gttacccaat gccatatgga ggctttggcc tggactgagg
     9961 cgctgggtat ttaaacgcta aaaccctctt gaaaaaataa agcgtattcc catattgaaa
    10021 atacagatgc ttgcagtttg aggtctgtga ttcatgttaa tcgcttaatt tttgaaagga
    10081 tcaacttatg cgtacttacg acttaacccc tttttataga accgctattg gttttgaccg
    10141 tttggccaaa ttactgaatg aaagccaacg taacgaaaac gatatcagct atcctcctta
    10201 taacatcgaa ttggttgacg agaatgccta caaaattgtg atggcagtcg ccggcttcca
    10261 gcaggatgag ttggagattg aaaccgaaca ccaggcattg cgtgtcacag gccgcaaagc
    10321 gaaaaaagag caggaaacca ctttcttgca ccgtggcatt gccacacgtg attttgaaca
    10381 caaattccag ttggctgacc atgtgaaagt caccggtgcc aatctcgaga atggcttgct
    10441 tactattgca ttattgcgcg aagtgccaga agcactgaag ccacg
//
LOCUS       DATMSK010000107         9733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_384396,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000107 DATMSK010000000
VERSION     DATMSK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384396"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VNZ84_03685"
     CDS             complement(<1..1205)
                     /locus_tag="VNZ84_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWU66087.1"
                     /translation="MKSSRIPVIVALAFAISSCSLVPEYFRPAAPVPETYPGQPENES
                     VTNMPVTEAPPLTWQTYFPDADLQKLIQVGLQHNRDLKTAVLRMDEAKATYGVQKADR
                     LPTVQGNLNYDRSRTVFSSSQAFEAELFRVGVGISEYEIDVFGRVKSLTQSALEQYLA
                     VAENRQAVQVTLVTEIATQYVNYLAIQAQLKVSQQTLADRQQMLQRTQRRFDAGLDQV
                     LDVKAAQIQVEQVQAQLAQWQRQLATTQNALYLLLGEPSQRTPLSVKPLEDLKLADVT
                     PGLPSSLLTRRSDIRAAEHQLKAANANIGAARAAFYPRLQLTTNVGLVNSDFAKLFSD
                     TGSNYWAFSPQLVIPIFNYGRNKASLKLAEARQHIEVVNYEKTIQTAFKEVMDVLSTR
                     TAIAEEAAS"
     gene            complement(1195..4305)
                     /locus_tag="VNZ84_03690"
     CDS             complement(1195..4305)
                     /locus_tag="VNZ84_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWU66088.1"
                     /translation="MSRFFIDRPIFAWVIAILIVLAGLIAIKQLPIAQYPDIAPPVVN
                     IAATYPGASAKVVEETVTSIIEREMNGMPGLMYVSATSNFGMASVSVTFKQGTNPDLA
                     AVDVQNRLKSVEVRLPEIVRRSGIMVEKSADSFQLIVSLTSEDNRYSEIDLGELSSAT
                     VMPTLKRVQGVGKVQSFSPEYAMRIWPDPQKMAGLNVSVTEISNALRSYNTRLTLGQV
                     GASGVPEGAPLSVSLEAEATLKTVEDFANVPIRSQADGSALLVKDVARVELGSSDYTY
                     SSKVNGKNAAGMAIKLAPGSNAVETIKRVKVAMEQMQPYFPPNVVFQLSYDSSAFVSI
                     SIKKVIQTLVEAVLLVFVVMYLFMQNLRATLIPTIVVPIALMGTFAVMYWLGYSINVL
                     TMFGVVLAIGILVDDAIVVVENVERLIVDEGLSPYEATIKAMSQISGAVVGITAVLVS
                     VFIPMAFFSGAVGNIYRQFSLALSVSISFSAFLALSLTPALCVTLLRHESGSKARFFV
                     WFNRSFQRFTERYTHLTGGLLQKPLRWLAVFALIVTLVGWLFMRLPSAFLPEEDQGNF
                     MVMVSLPQGATLAETAEVLGDMSRYIQAHEPVDIIFEVSGFSFYGTSTNSGMLFITLK
                     DWTERPKAEQQVQAIVDRVNQQFYGRPNLSIFAMNAPPLPELGSVGGFDMRLVDRASV
                     GIETLMKARDQLLAESAKQPELANVLFAGLYDTPVVNMQLNRNKANALGISLDEINDS
                     LATLLGSNYLGDFVYGNQVRRIIVQADGKQRQTLEDIKKIRLKAADGSLVPLSSIVTL
                     DWAMGSPQRTRFNGFPSFNINGGAGPGYSSGEAMQKMEKLVQGLGKGIGFEWAGQSLE
                     EKQSGSQATLLFGLSILVVFLVLAALYESWAIPFAVILVVPLGILGALLGVTLRGLPN
                     DIYFKVGLIATIGLSAKNAILIVEFAREHVLQGMPLLEASLLAAKERLRPIVMTSLAF
                     GVGVIPLAFSTGAASGAQVAIGTSVLGGIIAATLLAIFFVPLFFIVVGRWMHKPASQQ
                     KVSHEV"
     gene            complement(4331..5494)
                     /locus_tag="VNZ84_03695"
     CDS             complement(4331..5494)
                     /locus_tag="VNZ84_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU66089.1"
                     /translation="MSRFSVSTALVSTLVLLQLSACSKHQEAAQTAAAAPEVEVVAVQ
                     VVDIPDEVELPGRVEAYRIAEVRARVSGIVSKRLYQEGQVVKAGTPLFALNPEQLQAS
                     KLEADAELARTEANLVNANDKLQRYQALIGDQSVSQRDYRAAQAEAQLAKAEVAAAQA
                     KLNRAKLDLGYANVTAPIDGVARRALVTEGALVGKDEATHLTTVEQVNPVYVNFAQSS
                     SEVFALRKALRDGQLKGNDGQFKIELLLPDGSRYPSAGKLSFSDVSVNQTTDSVVMRA
                     IFDNPQQGLMPGAYVRLKIRQATNPHAILIPRDALIRDQFSSRVWVVNAKHELESREV
                     QTGAVIDKQWVVEHGLQAGEQVVVTNAATQVAGTKVTPKQVSATDKITKAVAP"
     gene            5622..6221
                     /locus_tag="VNZ84_03700"
     CDS             5622..6221
                     /locus_tag="VNZ84_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWU66090.1"
                     /translation="MARKTKEDAQKTRDQILDAAEHVFYRKGFTLTTMADIAEAAQLS
                     RGAVYGHYKGKLEVATAMAERVIQAASPFQQQPALSALLNLKTYCLQEIRSYIDPSSI
                     QRVLFFLYVGIDDSPELLQLRFAWEKKRIAQIDSLLRQAIAQKELSTEMDFDLISLYC
                     QSIIEGVFSIIYFGSLSESQRWQRAEQLCEYGLQRLSMP"
     gene            6424..8124
                     /gene="ybaL"
                     /locus_tag="VNZ84_03705"
     CDS             6424..8124
                     /gene="ybaL"
                     /locus_tag="VNZ84_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HWU66091.1"
                     /translation="MDHNLTLITTIAAAFALALIFGLIAEKLKLPALIGYLFAGICIG
                     PATPGFVADIEIASQLSEIGVMLLMFGVGLHFSVQDLMSVKRIALPGALAQMTIATLL
                     GMLVAHYWGWSMGNGLVFGLSLSCASTVVLLKALESRRILESMNGKIAVGWLVVEDMV
                     TVLVLVLMPALAGALGGNQSEVTNVTAIWETIGMTLLEVAGFVAVMLVVGKRVLPWLL
                     WYVAGTGSRELFTLTVIAVAIGVAFFASSLFNVSFALGAFFAGMIMRESEYSHRAAED
                     SLPFRDAFAVLFFVAVGMLFDPAILLKQPVQVLSVVAIIILGKSLAAVAIVLALRYPL
                     NTALTVAASLGQIGEFSFILAGLGVSLNLLSADGMSLVLAGALISIALNPLVFASIKP
                     LTNWIVNKSDVARRLSLRMDPYGELPMSTERKFLEGQVVLVGYGRVGKRIAQSLEASA
                     IPFVVAEQNRELVEKLRKRGQPAVSGDAADPMVLVQAHIANAAMLIIATPDSMNIRKM
                     VDIAKQLKPDIEVVIRTHHEDEYQRLQKELGEAVFFGEEELAKGMSAHVLQRFERHIL
                     "
     gene            8246..8389
                     /locus_tag="VNZ84_03710"
     CDS             8246..8389
                     /locus_tag="VNZ84_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66092.1"
                     /translation="MSDKNNDINIAIYDILSPNFECQFLVDVLRQPDARSMLIWKPGQ
                     QTA"
     gene            8343..9494
                     /locus_tag="VNZ84_03715"
     CDS             8343..9494
                     /locus_tag="VNZ84_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU66093.1"
                     /translation="MLAACSSGNQDNKPPKKTPEVGVVTVEQASQPIVADLPGRTSPF
                     LISEVRPQVSGIIRQRAFTEGALVKAGDLLYQIEPAPYQASYQSAAAALQKTEATLTT
                     LKLKADRYKELVKINAISRQDNDDAQAALLQGEADVASARAALSTAKINLDFTRVVSP
                     ITGYVSTSVVTPGALVTAEQTTALTTVQQLDPIYVDVIQSSSDLLKLKRDIASGKLST
                     TAQKEIPVRVVLEDGSTYAHAGKLKFNGISVNTASGAVTLRAVVPNPEGVLLPGMYVH
                     ALIDVAEDKTAILVPQRAVTRNTRGEPMVLVVDDAQKVVQQPIETSGTQGEQWRVTKG
                     LKAGDRVIMDGIQAINVGSEVKPVPFVATASTAETSAAPAKSPTVNPAR"
     gene            9513..>9733
                     /locus_tag="VNZ84_03720"
     CDS             9513..>9733
                     /locus_tag="VNZ84_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU66094.1"
                     /translation="MAYFFIQRPIFAWVIAIVIMLAGIGAIKWLPLEQYPDIAPPRVS
                     INANYTGAAADTIENSVTQVIEQYMKGID"
BASE COUNT         2332 a         2666 c         2518 g         2217 t
ORIGIN      
        1 ctagatgcgg cctcttcggc gatagcggtg cgtgtactta gtacatccat cacttcttta
       61 aacgcagtct ggatggtttt ttcgtaattg acgacctcaa tatgctgacg ggcctcagcc
      121 agtttgaggc tggctttgtt gcggccatag ttaaaaatcg ggatgactag ttgcgggctg
      181 aaggcccagt aattgctgcc ggtatccgag aacaatttgg cgaagtctga attgaccagg
      241 ccgacgtttg tggtcagttg caggcgtggg taaaacgccg cccgtgccgc gccgatattg
      301 gcgttggcgg ctttgagctg atgctcggca gcgcggatat ctgagcgccg agtgagcaag
      361 ctcgacggca agccgggcgt aacatctgcc agttttaaat cctcgagcgg tttgaccgat
      421 aacggcgtgc gttgcgaagg ctcaccgagc agtaaatata aagcgttttg cgtggtggcc
      481 agttggcgtt gccattgggc aagttgtgcc tgcacctgct cgacctggat ttgcgcggct
      541 ttcacatcta gcacttgatc cagcccggca tcaaaacggc gctgggtgcg ttgcaacatt
      601 tgctgccggt cagcaagggt ttgctggctg actttgagtt gtgcctgaat ggcgaggtag
      661 ttcacgtatt gagtcgcaat ctctgtcacc agggtgacct gcaccgcttg ccggttttcc
      721 gccacggcca ggtattgctc tagcgcggat tgcgtcaggc ttttcacgcg gccaaagaca
      781 tcaatttcat attcactaat gcctacacct acacggaaca gttcggcctc aaacgcctgg
      841 ctggagctga acacggtgcg gctgcggtca tagttcaggt taccctgaac cgttggtagc
      901 cggtcagcct tttgcacgcc ataagtcgcc ttggcttcat ccatgcgcaa cactgcggtt
      961 ttaaggtcgc ggttatgctg caagcccacc tgtatgagct tctgcaagtc ggcatctgga
     1021 aagtaggttt gccaggtgag aggaggtgcc tcagtgacag gcatattggt gacgctctcg
     1081 ttctcaggct ggccggggta cgtttctggc accggtgccg ctggcctgaa atattcaggc
     1141 accagcgagc aactgctgat ggcaaatgcc agtgcaacaa tgacgggaat acggctagac
     1201 ttcatgtgac accttttgct gggaggctgg cttgtgcatc cagcgcccca ccacaataaa
     1261 aaataacggt acaaaaaaga tggcaagcaa ggtggcagcg ataataccgc cgagcacgct
     1321 ggtaccaatg gccacttggg ctccagaggc tgcgccagtg gagaaggcta agggaatcac
     1381 gcccacacca aacgccagcg aggtcatcac aatcgggcgc agacgctctt tggctgccag
     1441 caagctggct tcaagcaaag gcatgccttg tagtacatgc tcacgtgcaa attccacaat
     1501 caggatagcg tttttggcag ataaaccaat cgtcgcaatc aggccaactt taaaataaat
     1561 atcgtttggc agtccacgca gggtcacccc cagtaacgca cccaagatgc ctaatggcac
     1621 caccaggatg acggcaaatg gaatcgccca gctttcatat aacgcagcca gcaccaggaa
     1681 caccaccaga atagacaagc caaacagcaa ggtcgcctgg ctgccggatt gtttttcttc
     1741 cagtgattgc ccggcccatt caaaaccaat gcctttaccc aggccttgca ccaatttttc
     1801 catcttctgc atggcctcgc cactgctata acctgggcca gcgccaccat tgatgttgaa
     1861 ggatgggaaa ccgttaaagc gtgtccgttg cggactgccc atggcccaat ccagcgtcac
     1921 gatagaagac aggggcacta aacttccatc cgccgcctta agacggattt tcttgatatc
     1981 ttccagcgtc tggcgctgtt tgccatcggc ttgcacaata atccggcgca cctggttacc
     2041 gtagacgaaa tcacccagat agttggagcc gagcaaggtg gctagggagt cattgatttc
     2101 atccagcgaa atacccagtg cattggcctt gttgcggttg agttgcatat tgaccaccgg
     2161 ggtatcgtac aagccggcaa agagcacatt ggccaattca ggttgcttgg ccgactctgc
     2221 cagcagttga tcgcgcgctt tcatcaaggt ctcaatgccg acgctggcac gatcaaccag
     2281 gcgcatatca aacccgccca cactgccgag ttctggcaat ggcggcgcat tcatggcaaa
     2341 aatacttaaa ttgggtcggc catagaattg ctggttaaca cgatccacaa tcgcctgcac
     2401 ctgttgttcg gctttaggcc gctcggtcca atccttgagc gtgataaaca gcatgccgct
     2461 attggtgctg gtgccgtaaa aactgaaacc gctgacttca aagataatat caaccggctc
     2521 atgcgcttga atataacggc tcatatcgcc cagcacctcg gcggtttcag ccagcgttgc
     2581 accttgtggc aatgacacca tgaccataaa gttaccctgg tcttcttccg gcaaaaaggc
     2641 acttggcaat cgcataaaca gccagccgac caaagtcaca atcaaggcaa agactgccag
     2701 ccagcgcaat ggcttttgta gcagacctcc agtcaaatgc gtatagcgtt ctgtaaagcg
     2761 ttggaagcta cggttaaacc agacaaaaaa gcgggcttta ctgccggact catgccgcaa
     2821 cagcgtgaca cataaagcag gcgtcagcga cagtgccaag aacgccgaaa agctgataga
     2881 gaccgacagt gccagtgaaa actggcggta aatattccct accgcgccac tgaaaaacgc
     2941 catcgggata aacaccgaca ccagtacggc agtaataccc accaccgccc cgctaatctg
     3001 tgacatcgcc ttgatggtcg cttcataggg cgacaagcct tcatcgacaa tcaggcgttc
     3061 gacgttttcc accaccacaa tcgcatcatc caccaggata ccaatggcca gcaccacacc
     3121 aaacatggtc aaaacgttga tggagtaacc cagccagtac atgacggcaa aggtgcccat
     3181 caaggcaatc ggcaccacga tggtcggaat cagcgtcgcc cgcaaatttt gcataaacag
     3241 gtacatgacc acaaatacca gcagcaccgc ctccaccagc gtttgaatca cttttttaat
     3301 cgagatagac acgaatgccg aggagtcata cgacagctgg aacaccacat ttggcgggaa
     3361 gtatggctgc atttgttcca tcgcgacctt gacgcgcttg atggtttcta ccgcattgga
     3421 acctggtgcc aacttgatcg ccataccggc cgcatttttg ccattcactt tagagctgta
     3481 ggtgtagtca ctgctgccca gctccacccg ggccacatct ttcaccagca aggctgatcc
     3541 atctgcctgg ctacgaatag gcacattggc aaagtcttct acggttttga gtgtggcctc
     3601 agcctccagt gacacgctaa gtggtgcgcc ctcgggcaca ccgctggcgc ccacctgccc
     3661 aagcgtgagg cgggtattgt agctacgcaa ggcattggaa atttcggtga cgctgacatt
     3721 caggcccgcc attttttgcg gatccggcca aatgcgcatg gcatattccg ggctgaagga
     3781 ctgcacctta ccaacgcctt gcacacgctt gagggtcggc atcacggtgg cggaagacaa
     3841 ctcacccagg tcaatttcac tatagcggtt atcttccgag gtcagggaaa cgatcaactg
     3901 gaaactatcc gctgattttt cgaccatgat gccgctacgg cgcacgattt ccggcaaacg
     3961 gacttccacg cttttgaggc ggttttgtac atccaccgcc gccaggtccg ggttggtgcc
     4021 ctgcttgaag gtcacactga cagaagccat gccaaaatta ctggtcgccg aaacatacat
     4081 cagacctggc atcccgttca tttcgcgctc gataatcgag gtcacggttt cttcgaccac
     4141 tttggccgat gcgcccggat aggtcgccgc aatattcacc acaggtggtg cgatatccgg
     4201 gtattgagca atcggcaact gcttgatcgc aatcaggcct gccagcacaa tcaggatggc
     4261 aatcacccag gcaaaaatcg ggcgatcaat aaagaaacgg ctcatgaatt cactttcctt
     4321 gggcggccgt ttaaggcgca accgctttcg ttattttgtc tgtggcactg acctgcttgg
     4381 gcgtcacttt tgtgcctgca acttgtgtcg cggcattggt caccactact tgctcgccag
     4441 cttgcaggcc gtgttcaacc acccactgtt tatcaatcac agcgccggtt tgtacttcac
     4501 gtgactccag ttcatgttta gcattgacca cccatacccg gctggagaac tggtcacgga
     4561 tgagcgcatc gcgcggaatc aagatggcat ggggattggt agcctgacgg attttgaggc
     4621 ggacataagc acccggcatc aacccctgtt gcggattgtc aaaaatcgcg cgcatcacca
     4681 cagaatctgt ggtctggttg acggagacat cagagaatga aagcttgccg gcactcgggt
     4741 agcgcgaacc atcaggcagc aacagttcaa ttttaaactg gccatcattg cccttgagtt
     4801 gcccatcacg cagtgcttta cgcaaggcga acacctcgga ggatgactgg gcaaaattca
     4861 catacaccgg attgacctgc tcgacggtcg tcaggtgggt cgcttcgtcc ttacccacca
     4921 gtgcgccttc agtcaccaac gcgcgacgtg ccacaccgtc aatcggggcg gtcacattgg
     4981 cgtagcccaa atccagcttg gcccggttga gcttggcttg cgcagctgcc acttctgcct
     5041 tggctaactg cgcttcggcc tgtgccgcgc gatagtcacg ctggctgact gactggtcgc
     5101 caatcaatgc ctggtaacgc tgcagtttat cgttggcatt caccaggtta gcttctgtcc
     5161 gcgccagctc ggcatcggct tccagcttgc tggcctgcaa ctgttctgga ttgagggcaa
     5221 ataatggcgt accggccttg accacctgcc cttcctgata gaggcgctta gagacaatcc
     5281 ccgagacccg cgcacgcact tctgcaatac ggtaggcttc gacacggcca ggcaactcca
     5341 cttcatcggg aatatcgacg acctggacgg ccaccacttc tacttcgggc gctgcggctg
     5401 cggtttgggc agcttcctga tgtttgctgc aggcggacaa ttgtaataac acgagcgtag
     5461 agactaaagc cgtgctgaca gaaaatcggg acatgaacat gccttcttac tatatggacg
     5521 tcaatattct atctgcacct ccataattaa tcaatcatga ctgattaatt attttttggt
     5581 atcatagcgc cattgtttga tggatgatcg ccctagaaat catggccaga aaaaccaaag
     5641 aagatgcgca aaaaacccgt gaccaaattc tggacgcggc tgaacatgtg ttttaccgca
     5701 aagggtttac cctcaccacc atggcagaca ttgccgaagc cgcccaactg tcgcgcggtg
     5761 cggtgtatgg tcattacaaa ggcaagctgg aagtggccac ggccatggcc gaacgcgtga
     5821 ttcaggccgc ttcaccgttt cagcagcaac ccgcgctcag cgccctgctg aacctcaaga
     5881 catactgcct gcaagagatc cgcagttata ttgaccccag ctcgattcaa cgggtgttgt
     5941 tttttcttta cgtggggatt gatgactctc ctgaactgtt gcaattgcgc tttgcctggg
     6001 aaaaaaagcg tattgcacag atagacagcc tgctcagaca agccattgca caaaaagaac
     6061 tgtcgacaga aatggacttt gatttaatca gcctgtactg ccagtccatt attgaaggcg
     6121 tgtttagcat tatctatttt ggcagcctca gtgaatcgca gcgctggcaa cgtgccgaac
     6181 aattatgcga atacgggctg caacgcctgt ccatgccctg attgccatag agacttaaag
     6241 atacaccgcg gcgattgacg gccacacctc ccttccggca agataaaggc cggatacatt
     6301 gataacaata aaattgttca acttttttgc ttgaacaagg tcagtgagac aaaccaccca
     6361 ggtttgcgat caatcctcac gacacacgaa acctataaca ataacttaag aaaacacaca
     6421 gctatggacc ataacctgac cctgattacc accattgccg ccgcttttgc gctggcattg
     6481 atatttgggc ttatcgccga aaaactcaaa ctccccgcac tgattggtta tttatttgcc
     6541 ggcatctgca taggaccagc aactcctggc tttgtcgccg atattgaaat cgcctcacaa
     6601 ctctctgaaa ttggcgtgat gctattgatg tttggcgtag ggttacattt ctccgtgcaa
     6661 gacttgatgt ctgtgaaacg gattgcgctg cctggtgcat tggcacaaat gaccattgcc
     6721 acgctgctag gcatgctggt cgcccattac tggggctgga gcatgggcaa tggcctggta
     6781 tttggcttgt cactctcttg tgcaagtact gtcgtgctcc tgaaagcgct ggaatccagg
     6841 cgcatcctgg aaagtatgaa cggcaaaatt gcggtcggct ggttagtggt cgaagacatg
     6901 gtgaccgtgc ttgtgctggt gctgatgcct gcattggcag gcgcattggg aggcaaccaa
     6961 agtgaagtga ccaatgtaac cgccatctgg gaaaccatag gcatgacact gcttgaagtg
     7021 gcaggttttg tagctgtgat gctggtggtc ggcaagcgtg tgctgccatg gttgctatgg
     7081 tatgtcgctg ggactggctc gcgtgagctg tttaccctga ccgtgatcgc cgtggccatc
     7141 ggcgtggctt tctttgcctc gtcactgttc aatgtttcgt ttgcacttgg cgcgtttttc
     7201 gccggcatga tcatgcgcga atccgaatac agccatcgag cggcagaaga ctcgctaccg
     7261 tttcgggatg cgtttgcggt cttgtttttt gtggctgtcg gcatgctgtt tgatccggcc
     7321 atcttactca aacaaccggt acaagtattg tcggtagtgg caattattat cctgggcaaa
     7381 tcgctggcag ccgtggccat cgtgctggca ctgcgctacc ccttgaatac ggccctgacg
     7441 gtggccgcca gcctggggca aattggcgaa ttctcattta tcctggcggg attaggcgtg
     7501 tcactcaatt tgctatccgc cgacggcatg agcctggtac tggcaggtgc attgatttca
     7561 atcgcactga atccattagt gttcgcttcc atcaaaccgt tgaccaactg gatcgtcaat
     7621 aagtcggatg ttgcccgtcg attatcgtta cgcatggatc cctatggcga actacccatg
     7681 agcacagaac gcaaattcct ggaaggtcag gtggtgctgg tcggctatgg ccgggtgggt
     7741 aaacgtattg cgcagtcgct cgaagcctcg gccattccgt ttgtggttgc cgaacaaaac
     7801 cgggaactgg tagaaaaact gcgcaagcgt ggtcaaccag ccgtttccgg tgatgctgcc
     7861 gaccccatgg tgttagtcca ggcgcacatt gccaatgccg ccatgctgat tattgccacg
     7921 ccggactcca tgaacatacg caagatggta gacattgcca aacaattgaa accggatatc
     7981 gaggtggtga ttcgtacgca ccatgaagat gaataccagc gcctgcaaaa agagctgggg
     8041 gaagcggtat tttttgggga agaagagctg gccaaaggca tgtccgccca tgtcttgcag
     8101 cgatttgagc gacatatcct gtaaacaaca aaggctacat aagtagcctt tgttgtttat
     8161 acgctgcaca cattattatc gcgatgaatg gatgatcgta gatacgtatc gttacattta
     8221 tacctgatca aaacacagta caatcttgtc agacaagaat aacgatatca atattgcaat
     8281 ttatgacatc ctctccccga atttcgaatg ccagtttctg gttgatgtgc tgcgccagcc
     8341 tgatgctcgc agcatgctca tctggaaacc aggacaacaa accgcctaaa aagacccctg
     8401 aggttggcgt cgtgaccgta gaacaagcga gccagccgat tgtggccgac ctgcctggcc
     8461 gtaccagtcc attcctgatt tctgaagtgc ggccacaagt gagtggcatt atccgccaac
     8521 gtgcattcac cgagggggca ctggtcaagg ctggtgattt gctctaccag attgagccag
     8581 ccccttacca ggccagttac caaagcgctg cagcggcatt acaaaaaacc gaagcaaccc
     8641 tgaccacgct caaactcaaa gcggaccgct acaaagaact ggtcaaaatc aacgccatca
     8701 gccgacagga caacgacgac gcacaagccg ccctgctgca aggggaagca gatgtcgcct
     8761 cagccagggc agcgctttcg actgcaaaaa tcaacctgga cttcacccgc gtcgtgtccc
     8821 ctattacagg ttatgtgtct acgtcggttg tcacaccagg tgccctggtc acggctgagc
     8881 aaaccaccgc cctcaccaca gtacagcaac tggatccgat ttatgtggat gtgatccagt
     8941 ccagcagcga tttgcttaaa ctgaagcgag acattgccag cggcaaactg agcaccacgg
     9001 ctcagaaaga aatcccggtc cgcgtcgtgc ttgaggacgg atccacctac gcccatgccg
     9061 gaaaactcaa gttcaatggc atcagcgtca acacggcttc tggcgcggtc accttgcgtg
     9121 ccgtggtgcc caacccggag ggcgtactgt tgcccgggat gtatgtacac gcattgatcg
     9181 acgtcgccga agacaaaaca gcgatcctgg ttccgcaacg cgccgtgacc aggaatacac
     9241 gcggagaacc catggtgtta gtggtggatg atgcgcaaaa ggtggtacaa caacccatag
     9301 aaaccagcgg tacccaaggt gagcagtggc gtgtcaccaa aggcttaaag gctggtgacc
     9361 gtgtcatcat ggacggcata caggccatta atgtaggcag tgaagtgaag ccggtgccct
     9421 ttgtggcaac agccagcacc gccgaaacat ctgcggcccc ggcaaaatca cctacagtga
     9481 acccggcccg ctaacgcgaa gagattttat ttatggcgta cttttttatt cagcgcccga
     9541 tctttgcctg ggtgattgcg attgtcatta tgctggcagg cataggggcc atcaaatggc
     9601 taccccttga gcaatacccg gatatcgcgc cccctcgcgt ctcgatcaat gccaactata
     9661 ccggtgccgc tgccgacacc attgaaaact cggtcaccca ggtgattgag cagtacatga
     9721 aaggcattga taa
//
LOCUS       DATMSK010000108         6006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_266751,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000108 DATMSK010000000
VERSION     DATMSK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266751"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..2052)
                     /locus_tag="VNZ84_03725"
     CDS             complement(163..2052)
                     /locus_tag="VNZ84_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU66095.1"
                     /translation="MIQFKQLTFSRAGKALVEHASFQLHPGHRVGLTGANGAGKSTLF
                     ALLRGELVADEGELLMPPQWVIAHVAQETPALAQSALEYTLDGDEELRLLQTNLANEH
                     QDPLQLAEWHQRLADIDGYSAEARASALLAGLGFSQSQLSQPVSDFSGGWRMRLNLAR
                     ALMCRSDLLLLDEPTNHLDIEAVIWLEGWLNAYRGTLLLISHDREFLDNTVNHILHIE
                     QQQLTLYRGGYSDFERQRAEKLTLQQASYQKQQQQIAHLQKYIDRFRAQATKARQAQS
                     RIKALERMERISAAHVDSPFSFGFRDTGASPDPLLVMNHASLGYTDKPLVKLVHLAIR
                     PGERIGLLGKNGAGKSTLIKALADSATLLAGERVEGKDLRIGYFAQHQLEQLIASDSP
                     LQHLQRLDPLTREQELLDFLGSFDFRGEMAKTPCGPFSGGEKSRLALAMLIWQRPNLI
                     LLDEPTNHLDIEMRHALSMALQAYEGGMVMVSHDRTLLATCCEKFVLVAAGQATVFDG
                     DLEDYKQFLMQAVQTENASSEPIAPKQNSYHQQKAERQARLAERRPLLKSLTSLENQL
                     AQLEQEKQVLEDRLADSEIYNAEHREALQALLIAQAQNQKQLDAVEMQWLDIQEKLEA
                     LPEFD"
     gene            2229..>6006
                     /locus_tag="VNZ84_03730"
     CDS             2229..>6006
                     /locus_tag="VNZ84_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HWU66096.1"
                     /translation="MPLQKIKRWLVSFLIVIFVLLAGIIGVIQFYVFPHIDEYRGKIE
                     QALTESLKQQVTIAHIAIRWRGLAPQVTLGKVTLFDTQHRPALELSKVTTRLSWSSLV
                     TLSPALVSLSIDAPNLVVRRTADGELFLAGMSMSRQGNPAFTNWLLAQRRIKVSNATV
                     TWLDEKRQAPPLLLEDLNIEILTPVWQRLLNRHSIHIDTRVSTGTQQRITLESVVVGS
                     DVAKPEEWHGEISLRLPDTDMAAWGPWLDLPVKVKAGKGNLTTTMAFDTMQLQRLAAQ
                     LQLKDLAVTPANETQTFVAKTMSGELRWQRVEQRFMLTLKHFSADIQPGLLLEDTNGE
                     LQWDSHGHEGVLTTKKFSLAQGNLFAQWLPPTSAAARYLENMAPAGNASRIKARWLYQ
                     QDQWRDYALDASFSGLQSKAYDNIPGLDNWSGQLSVRPNTGSIELDTSKASIELAGLL
                     RWPLPIDRLQGEIEWNHNGQKTLISTRDLTFSNQHLALRTNLEYQLGAAGGDTIQLQS
                     QIERGDAKFAPFYYPLILGETTLHWLDTSILSGQVKRGEVIVKGKVKDFPYVNEAHQS
                     DPSLGLFRVTAQVDQASLEYGTGWPVIQNIDALLKFEGKRMDITVKNGTTVGQHITQA
                     QVEIPRLDADWPMLNIKSAATGTLEQGIKFVNTSPVREVTMGFTDYVKANGKAELKLA
                     LQVPLNDVDASQFQGDYNIKNGHIAANPQIAMPEMSNINGHLKFNEKGLQAQGIQLSM
                     FDNPASISLSTRPDKSVIIQGSGRITDAALRKLDNNPITRALHGTTDWKSNLLIQAPN
                     IRLDIRSQLIGMAINLPEPAKKSVETAANLSIRLRQESNQPDKIAIHYNDWIHANFFR
                     DPNNQTKPISAGEIAINTPPRLPSGEGINLRADFQKLDVDEWLAYLNQGGDNQASASS
                     SLALNQIELTAATLHVFDRNLHQIKLNISPESERLKLAIQSQELAGDADWLPGKPSKL
                     VAKLSYLRIPRTMDTDNPQNPAEVRRLDLTYPEVDITAQDFQFGEKQLGSLDLKAFNS
                     GESWVIQRMNLTNPDSQLTADGTWRNSVRNPQTQLKFNLTSSNLGKTLQRFQPGGEMV
                     KGGTGNMSGQLGWPGSPHEFAVERLDGNFTMQLEKGQILKVQPGVGRLLGLLSLQSLP
                     RRLTLDFRDLFSEGFAFDKITSTANIRDGILRSDDLFMTGPAAEARIKGETNLKTETQ
                     RLRVKVVPHISDSVSLAALAGGPIVGVAAFVAQKLLKDPLNKISASEYMITGTWD"
BASE COUNT         1541 a         1676 c         1454 g         1335 t
ORIGIN      
        1 caggtaatgg gtcgaatctt caatatccgt attagccagc gcagcaactg cctctgcttc
       61 cgacaagcta tctgcccgcg tctgggcctg gcacaactga tgcattaacc tcaaacgctg
      121 ttcataagtc atttccataa tgattccttc aatatcattg tgctaatcaa attctggcaa
      181 agcttccagc ttttcctgta tatccagcca ttgcatttca accgcatcca gttgtttttg
      241 attttgcgcc tgggcgatga gcaatgcctg caatgcttcg cgatgctcgg cattataaat
      301 ttcactgtca gccaagcggt cttctaacac ctgtttttct tgctccagtt gggccaactg
      361 gttttcaagt gaggtcaatg acttgagcaa aggcctacgc tcagccaacc gcgcctgcct
      421 ttctgccttt tgttgatgat agctgttttg cttaggcgca ataggctcgc tggatgcgtt
      481 ctctgtctgc acggcttgca tcaagaactg cttgtaatct tccagatctc catcaaacac
      541 tgtcgcttga cctgcggcca ccagcacaaa tttctcgcaa caggttgcca gtagcgtacg
      601 gtcatgcgac accatcacca tgcctccttc atacgcctgc aacgccatgg acaaggcatg
      661 acgcatttca atatccaggt gattggtcgg ttcatccagc aaaatcaagt tggggcgctg
      721 ccaaatcaac atggctaatg ccaggcgaga cttttcgcca ccagaaaatg gtccgcaagg
      781 cgtcttggcc atttcgcccc tgaaatcaaa actacccagg aaatcaagca actcttgctc
      841 cctggtcaaa gggtccagcc gttgcaaatg ctgcaatggc gagtcactgg ctatgagttg
      901 ctccagttga tgctgtgcaa aatagccaat ccgcaaatcc ttgccttcca cgcgttcacc
      961 tgccagcaag gtggcagaat cggccagcgc ttttatcagg gtagatttac ccgccccgtt
     1021 cttgcctaat aagcctatac gttcccctgg gcgtatcgcc aggtgtacca acttgaccag
     1081 gggcttatcc gtgtaaccaa gcgacgcatg attcatcacc agcaatgggt ctggtgatgc
     1141 gccagtatcc cggaagccaa aactaaacgg cgaatctaca tgcgcggcac ttatgcgctc
     1201 catgcgttcc aaagccttga tccggctttg cgcctgacgc gccttggtcg cctgggcgcg
     1261 aaaacggtca atatactttt gcagatgcgc aatctgttgc tgctgcttct ggtaactggc
     1321 ttgctgcaag gtcagttttt cagcacgctg ccgttcaaaa tcactatatc caccacggta
     1381 taaagtcagt tgttgctgct cgatatgcaa aatgtggttg accgtgttat ccaagaactc
     1441 gcggtcatgc gaaatgagca gcaaagtgcc gcggtaggcg tttaaccagc cttcgagcca
     1501 aatcactgcc tcaatatcca agtggttggt cggctcatcc agcaggagca aatcagaacg
     1561 gcacatcaag gcacgcgcca gatttaaacg catgcgccaa ccaccagaaa agtcactgac
     1621 aggttggctt aattgcgact gggaaaaccc caagccagcc agcaaggcac tggccctggc
     1681 ttccgcacta tacccatcaa tatcggccaa tcgctgatgc cactccgcca gttgcagcgg
     1741 atcctgatgc tcattggcaa ggtttgtttg caacaggcgt aactcttcgt cgccatccag
     1801 tgtgtattca agagcagact gtgccagcgc aggcgtttct tgcgccacat gtgcgatcac
     1861 ccattgaggc ggcatcaaca actcaccttc atcggcgacc aactcaccac gcaacaaggc
     1921 aaacaaggtg gattttcccg cgccatttgc accggtgagg ccaacgcggt gacccggatg
     1981 caactgaaaa ctagcatgtt caaccagtgc tttacccgcc ctggaaaatg tcagttgctt
     2041 aaactgaatc actggtcatc actttcattt aaaccgcaaa ttgcaaaaga gtggcatttt
     2101 aaacgaatca ttcatgctcc ggctgaataa ttcggcgaca actgtgcgct tctactgcgg
     2161 tgacgcaact gttttcaagt aaaatagtca gttaaacaat aacaccgtcg cctctgctta
     2221 acgcccttat gcctctgcaa aaaatcaaac gctggctggt ttcattcctg attgtcattt
     2281 ttgtcctatt ggcaggcatt attggcgtca ttcaattcta tgtttttcca cacattgacg
     2341 aataccgtgg aaaaattgag caggcactta ctgagtcact caagcaacag gtgaccatcg
     2401 cccatattgc gatccgttgg cgcggcctgg cgccacaagt cacgctgggc aaggtcaccc
     2461 tgtttgatac ccagcaccgc ccggcgcttg aactctccaa agtgaccacg cgcctttcct
     2521 ggagcagcct ggttacgctg tcacccgcac tggtcagcct cagcatcgat gcccccaacc
     2581 tggtggtgcg gcgaacagcc gacggcgaat tattcctggc cggcatgtcc atgtccagac
     2641 aagggaatcc agcattcacc aactggctat tggcgcaacg gcgcatcaag gtatcaaacg
     2701 cgaccgtcac ctggttagat gaaaaaagac aagcaccgcc tttattgctc gaagacctga
     2761 atattgaaat cctgacgccg gtctggcaac gcttgctgaa ccggcacagt atccacattg
     2821 acacccgcgt ttcaaccggc acccagcaaa gaattacact ggaatctgtc gttgtgggca
     2881 gcgatgtcgc caaaccggag gaatggcatg gcgagatctc attacgcctg cccgacacag
     2941 atatggcagc ctggggccca tggctggacc ttcctgtaaa agtgaaagct ggcaaaggca
     3001 acctgacgac aaccatggca tttgatacca tgcaattgca acgcttggct gcacagctgc
     3061 aactcaagga tctggcggtg acgcctgcca atgaaacaca gacgtttgtg gcaaaaacca
     3121 tgtccggtga actccgctgg caacgcgttg aacaacgctt tatgctcaca ctcaagcatt
     3181 tctcagccga tatccagcca ggactattgc tggaagacac aaatggcgaa ctacaatggg
     3241 atagccatgg ccatgaaggc gtgctgacca caaaaaaatt cagcctggcg caaggtaacc
     3301 tgtttgccca atggctcccc cctacatcgg cagcggcacg ataccttgaa aacatggcgc
     3361 ctgccggcaa tgcttcgcgt atcaaggctc gctggttata ccagcaggat caatggcgtg
     3421 attatgcgct agatgcctct ttcagcgggc ttcagagcaa agcctacgac aacattcccg
     3481 gcctggataa ctggtccggt caactctctg tccggccaaa tacaggcagc attgaactgg
     3541 ataccagcaa agccagtatt gaactggcgg ggctgttacg ctggccatta ccaatcgacc
     3601 gcttgcaagg tgaaatcgaa tggaaccata acgggcagaa aacattaatt agcacacgcg
     3661 atctcacctt tagcaaccag cacctggcct tgcgtaccaa tctggaatac caactcggtg
     3721 cagccggtgg cgataccatc caactgcaaa gccagattga gcgcggggat gccaagttcg
     3781 caccgtttta ctacccactg attctcggcg aaacaacgct ccactggctg gatacctcta
     3841 tcctaagcgg ccaagtgaag cgtggggaag tcattgtcaa aggcaaggta aaagattttc
     3901 cttacgtcaa tgaagcacat caatcagacc cttctctcgg cctgttccgt gtcacggcac
     3961 aagtggatca agccagcctg gaatatggta ccggctggcc ggtgatccag aatatcgacg
     4021 cattgctcaa atttgagggc aagcgtatgg atattacggt taaaaacggc accacggttg
     4081 gccagcatat tacgcaggcc caggtggaga ttccgaggct ggatgcagat tggcccatgc
     4141 tgaatatcaa atctgccgcg accgggactc ttgagcaagg tatcaagttc gtaaatacca
     4201 gcccggtgcg agaagtcacc atgggcttta ccgattatgt gaaagccaac ggcaaggccg
     4261 agctcaaact ggcgttgcaa gtgccattga atgatgtgga tgccagccag ttccagggcg
     4321 actacaacat taaaaacggg cacattgccg ccaacccgca aattgccatg cctgaaatga
     4381 gcaacatcaa cggccatttg aaattcaatg aaaaaggact acaagcgcaa ggcattcaac
     4441 tatccatgtt tgataacccg gccagtatca gcctcagtac acgtcctgac aagtctgtca
     4501 tcatccaggg cagtggccgc atcacggatg ccgccctccg caagctggat aacaacccta
     4561 tcaccagggc attacatggc accactgact ggaagagcaa tttactgatt caagcgccta
     4621 atatccggct ggatatccgt tcgcaattga taggcatggc aattaacctg cctgaaccag
     4681 ctaaaaaaag cgtggagact gctgccaacc tgagcatcag gttacgccag gaaagcaacc
     4741 agcccgataa aattgccatt cactataacg actggattca tgccaacttt ttccgcgacc
     4801 ctaataacca gaccaagccc atcagtgcgg gtgaaattgc catcaacacc ccgcctcgct
     4861 taccttccgg tgagggcatc aacttacggg cagactttca aaagctggat gtcgatgaat
     4921 ggctggccta cctgaaccag ggtggtgaca atcaagcctc tgcgtcctcc tcactggcgt
     4981 taaatcaaat tgagttgact gcagcgacct tgcatgtgtt tgaccgcaac ttgcatcaaa
     5041 tcaagttgaa tatttcacct gaaagtgaac gcctgaaact ggcgatccag agccaggaac
     5101 tggcaggtga cgcagactgg ctgcccggca aaccaagcaa gctggtcgca aaattgagtt
     5161 atctgcgcat cccgcgcacc atggatactg acaatcccca gaatccggcg gaagtccgcc
     5221 ggttggatct aacttatcca gaagtcgaca tcacggcgca ggacttccag ttcggcgaaa
     5281 aacaacttgg cagtctggac ctgaaggctt tcaatagcgg tgaaagctgg gtgatccaac
     5341 gtatgaacct caccaatcct gatagccagc tcacggcgga tggcacctgg cgtaattcgg
     5401 tacgtaatcc gcaaacacag ctcaagttca acctgacctc cagcaacctg ggcaaaacac
     5461 tgcaacgctt ccagcctggt ggtgaaatgg tcaaaggggg cacgggcaat atgagtggcc
     5521 aactgggctg gccaggcagc ccgcacgaat ttgccgtaga gcggctggat ggcaacttta
     5581 ccatgcagct ggaaaagggc cagatcctca aagtccagcc tggcgtaggc cgcctgctag
     5641 ggctgttgag cttgcagagt ttgccacgcc gcttgacgct ggatttccgc gacctgttta
     5701 gtgaaggctt tgcgtttgac aagatcacct ctaccgcaaa tattagggat ggcattttgc
     5761 gcagtgacga cctgtttatg accggccccg cagcagaggc gcgcatcaag ggcgaaacca
     5821 atttaaaaac agaaacacaa cgcttgcgcg tgaaagtggt gccgcatatc agtgattccg
     5881 tatccttggc agcgctggca ggcggaccga ttgtcggcgt ggccgccttt gttgcacaaa
     5941 agctgctcaa agacccgctt aacaaaatca gtgccagtga gtatatgatt acaggcacct
     6001 gggaca
//
LOCUS       DATMSK010000109         7108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_196253,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000109 DATMSK010000000
VERSION     DATMSK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196253"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /gene="rluD"
                     /locus_tag="VNZ84_03735"
     CDS             <1..1053
                     /gene="rluD"
                     /locus_tag="VNZ84_03735"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HWU66097.1"
                     /translation="GGQRLDLALQQMLPDHSRSRLQAWIKEELVLLDGKVTTAKTKVW
                     GGEKLDITPPKNAQENAFEPEDIPLDVVYEDDALIIINKPAGLVVHPAAGNWSGTLLN
                     ALLFHWPLLKDVPRAGIVHRLDKDTSGLLVVAKTLEAQTSLVRQLQARTVKREYRAIV
                     WGQLWRNGKVDQPIGRHPHQRVKMAVVRRGKPAVTHYEILERFGTNTYLRCNLETGRT
                     HQIRVHLQHLKAPMVGDPLYGIGNIIPHKMMTPELREVIGNFKRQALHAIKLGLIHPV
                     TNEPMEWQIELADDMRELLEAMRVTEPVEEVPLVDFNVDENGLFVGDDDEDWGDDEFD
                     EELDADLEDEAWEDEE"
     gene            1056..1784
                     /gene="pgeF"
                     /locus_tag="VNZ84_03740"
     CDS             1056..1784
                     /gene="pgeF"
                     /locus_tag="VNZ84_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWU66098.1"
                     /translation="MANLPLIIPEWPAPANVKAFQTTRHGGVSTGVYASLNLGDHVKD
                     SPQHVAANRQLLSAYMPSEPVWLNQVHGVRVIDAALSSCLESADASFTNGKQVVCVTM
                     TADCLPVLLCDQAGTAIAAIHAGWRSLCDGVIEEAVTAMTVSPSELMAWLGPAIGPDA
                     FEVGSEVRAQFMAQDMQAESAFKPKGNKWLGDLYAIARQRLQTLGVTQVYGGRRCTYS
                     EPETFFSFRRDGDTGRMGTFIWLE"
     gene            1837..2484
                     /locus_tag="VNZ84_03745"
     CDS             1837..2484
                     /locus_tag="VNZ84_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HWU66099.1"
                     /translation="MKQHLIFDLDGTLIDSAPSILECFGLAFSTTNTPLAARLTHDVI
                     GPPLMETLKRLSGSEDSALLNALAAAFKQHYDTTGCLQSVVFEGVNTMLQALKDSGYQ
                     LYIATNKRFYPTDKIMAHLGWQAFFDGVYALDYFNPPLKTKAEMIGRVVADNGLAIQD
                     CLYIGDRLEDGLSADANQMDFALVSWGYAGDVGSRKVHWHSCAQVQALPALVATL"
     gene            2491..2916
                     /locus_tag="VNZ84_03750"
     CDS             2491..2916
                     /locus_tag="VNZ84_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66100.1"
                     /translation="MLKHLRIRRHPATSGKVFDQPLSGKRAWPMLLLATALVALIAIG
                     IYVDWDARVLTGVALLVGLVSGLFVWLIGVIGLVPLIGPMIVKVLSLSFIWLLNALGY
                     LVSYIAIRRGYSRDVLTYRGLTMALLIGIVIGYIVAQFI"
     gene            3023..3940
                     /locus_tag="VNZ84_03755"
     CDS             3023..3940
                     /locus_tag="VNZ84_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWU66101.1"
                     /translation="MTALNTLFSDISPFTGMVLACLLGGVLSVCVAALFALNAKKHWV
                     PLLVSYAIGALLAAVFLEILPHAIETSPNIEAMTATMLFGVLLFFTLEKLVLWRHCHG
                     EHCEMHAMHDEAHCPELHPEQQGSKKPQGAIKFKLPQAQPAQVVGHHHAHAHDGGRSG
                     LMIMVGDTFHNFVDGILICAAFMVDMQVGLVTALAIIAHEIPQEVGDFLILLHSGYSK
                     KKAFLFNIASSLATLAGGLIAYLGLNLVEHWVPYILSLAAASMLYVAVADLIPGLHKR
                     TALRDTLSQLLLIVLGVASVAVTKWLVEG"
     gene            3953..5287
                     /gene="rimO"
                     /locus_tag="VNZ84_03760"
     CDS             3953..5287
                     /gene="rimO"
                     /locus_tag="VNZ84_03760"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWU66102.1"
                     /translation="MQSSPKVGFVSLGCPKAGSDSERILTQLRAEGYEISNSYEQSDL
                     VVVNTCGFIDSAVQESLDAIGEALNKNGKVIVTGCLGAKKDVVTGAHPSVLAVTGPHA
                     LEEVMGHVHTHLPKPHDPYTDLVPPQGIRLTPKHYAYLKISEGCNHRCSFCIIPSMRG
                     DLVSRPIGDVMNEAESLVNAGVSEILVISQDTSAYGVDMKFRKGFWNGRPIKTHMTEL
                     SKSLGELGVWVRLHYVYPYPHVDDVIPLMAEGAILPYLDVPLQHASPRILKSMKRPAS
                     SENNLARIKAWRDICPEITIRSTFIVGFPGETEDDFNMLLDFMEEAQLDRVGCFAYSA
                     VDGAAANALPDQVAEEVKKERLSRFMEVQERISAAKVAAKVGSLQTVLVDELTEDEDG
                     NVIAIARTKGDAPEIDGIVYLQDAEGLNPGDFVDVQIVDSQGHDLIGAPPEF"
     gene            complement(5347..5682)
                     /locus_tag="VNZ84_03765"
     CDS             complement(5347..5682)
                     /locus_tag="VNZ84_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66103.1"
                     /translation="MSHPSSSNIEIHVGELYGKPAVSGQTSTIYHVIAVHKLGITLQN
                     MDNPLSQFETTASKLLQSGYVRLSQTPYVNLSQGNKRKSTQQKLKRCPYTLDAFAERA
                     DFERPAVSV"
     gene            5810..>7108
                     /gene="gltX"
                     /locus_tag="VNZ84_03770"
     CDS             5810..>7108
                     /gene="gltX"
                     /locus_tag="VNZ84_03770"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWU66104.1"
                     /translation="MSQAVKTRFAPSPTGYLHIGGARTALFSWAYAKRHGGKFILRIE
                     DTDVARSTPEAVQAILDGMAWLQLDHDEGPFYQMQRMDTYKKIIQKMLDEGHAYYCYC
                     SKEELDALRESQMQQGLKPRYDGRWRPESGKVLPEPPKDVPPVVRFKNPTDGVVAWDD
                     MVKGRIEIANAELDDLIIARADGTPTYNFCVVVDDWEMGVTQVIRGDDHVNNTPRQIN
                     MLQALGAQVPQYAHLSMILGDDGQKLSKRHGAVSVMQYHEDGYLREAVLNYLARLGWS
                     HGDDEVFSMEQFCEWFDLDHITPSAAQFNTEKLNWLNAHYIKTLPLERIADEVKPRLA
                     ALGITEIQSPDLLAVLNLYRERANNLNQLAKEIAFFYQMPAINEADAVKHIQEETAAY
                     IESFLQKTQDSDWAADKLHDALNQVVAEQQIKFPRLAMPLR"
BASE COUNT         1647 a         1599 c         1995 g         1867 t
ORIGIN      
        1 ggcgggcagc gtctcgacct ggctttgcag caaatgttgc ctgatcattc ccgcagccgt
       61 ttgcaggcat ggatcaaaga ggaactggtc ttgctggatg gcaaggtgac cactgccaaa
      121 accaaagtat ggggtggtga aaagctcgat attactccgc ctaaaaatgc gcaggaaaat
      181 gcttttgagc cagaagatat cccgctggat gtggtctatg aagacgatgc attgatcatt
      241 attaataaac ctgccggcct ggtcgtgcat ccggctgcgg ggaactggag tggtacgcta
      301 ctcaatgcct tattatttca ctggccattg ctcaaggatg ttccgcgcgc tggcattgtg
      361 caccggttgg ataaagacac cagtggtttg ctggtggtgg ctaaaacact ggaagcgcaa
      421 accagcctgg tgcgtcaact gcaagcacgt acggtcaagc gtgaataccg cgccattgtc
      481 tgggggcaat tgtggcgtaa tggcaaggtg gatcaaccca tcggccgtca tccgcatcaa
      541 cgcgtcaaaa tggcggtcgt ccgtcgcggc aagccagcgg taacgcatta cgaaatcctt
      601 gaacgttttg gcaccaatac ctatctgcgc tgtaatctgg aaaccggccg tacgcaccag
      661 atccgtgtac atttgcagca tctaaaagcg cctatggtgg gcgatccact ctacggtatc
      721 ggcaatatta tcccgcacaa gatgatgacg cccgagttgc gtgaggtgat tggtaacttt
      781 aaacgccagg cactgcatgc gatcaaactg gggttgattc acccggtgac aaatgagccc
      841 atggagtggc agatcgaact ggcagacgat atgcgtgagt tactggaagc catgcgtgtg
      901 actgagccag tagaagaagt gccgttggtt gatttcaatg tcgatgagaa tggcctgttt
      961 gtgggtgacg acgatgaaga ctggggcgac gatgagtttg atgaagagtt ggatgctgac
     1021 cttgaggatg aggcttggga agatgaagaa taatcatggc taatttgcca ttgatcattc
     1081 ctgaatggcc tgcgcctgcc aacgtgaagg catttcaaac cacgcgtcat ggtggcgtga
     1141 gcactggcgt ctatgccagc cttaatctgg gtgaccatgt caaagactcg ccgcagcatg
     1201 tggctgccaa ccggcaattg ctcagtgcat atatgcctag cgaaccggta tggctgaatc
     1261 aggtgcacgg tgtgcgcgtg attgatgccg ctttatcctc ttgcctggaa agtgccgatg
     1321 ccagctttac caacggcaag caggtggttt gcgtcaccat gaccgcagac tgtctacccg
     1381 tgttgttgtg tgaccaggcc ggcactgcca ttgcggctat tcatgctggc tggcgcagtt
     1441 tgtgtgatgg tgtcattgaa gaagcagtga ctgccatgac cgtgtccccc agtgaactta
     1501 tggcctggct ggggcctgcg attggtccgg atgcgtttga agtcggcagt gaagtgcggg
     1561 cgcagtttat ggcgcaggat atgcaggctg agtctgcatt caagcccaaa gggaataagt
     1621 ggttgggtga cttatatgct atcgcccggc agcgcttaca aacgctaggc gtcacacagg
     1681 tgtatggcgg caggcgttgc acctatagtg agccggaaac cttttttagc ttccgccgcg
     1741 atggtgatac tggccgtatg ggtacattta tctggttgga ataaatacat gctggcaaag
     1801 tggcgtcggt gacaatatca ataaaaacga ttttttatga aacagcattt gatttttgat
     1861 ctggatggga cgctgattga ttctgcgccc agtattctag agtgttttgg cctggcgttc
     1921 agcacgacta acacgccgct agctgcacgt ttgacgcatg atgtgatcgg cccgccattg
     1981 atggaaacgc tcaagcggct ttccggcagt gaagatagtg cattgctaaa tgcactggca
     2041 gcagcgttta aacagcatta cgatacgacg ggttgtttgc agtcggtcgt gtttgagggt
     2101 gtaaacacca tgctgcaagc gctgaaagat agtggctatc agctttatat tgctaccaac
     2161 aagcgctttt atccgacaga taaaatcatg gctcatctgg gttggcaggc gtttttcgat
     2221 ggcgtttatg ccttggatta ttttaatccg ccgcttaaaa ccaaagccga gatgatcggg
     2281 cgcgtagttg ccgataacgg cctggcgata caggattgtt tgtatattgg tgatcgcctc
     2341 gaggatgggc tgtcggcaga tgctaaccag atggattttg cactggtcag ttgggggtac
     2401 gcaggtgatg taggttcgcg caaagttcac tggcatagct gtgcacaggt gcaagcgtta
     2461 cctgctctgg ttgcaacgct ataatattgt atgctcaagc atttacgtat tcgccgccat
     2521 ccggcaacgt caggcaaggt atttgaccag ccactgagcg gtaaacgtgc atggccaatg
     2581 cttttgctgg caacggcctt ggttgcgtta atcgctatcg gcatttatgt ggattgggat
     2641 gcgcgcgtgc tgactggcgt ggcacttttg gtcggcttgg tgtccgggtt atttgtttgg
     2701 ttaatcggcg tgattgggct agtgccattg attggcccaa tgattgtcaa agtgttgagt
     2761 ttgagtttta tctggctact taatgcgctg ggctatttgg tgtcatatat cgcgatccgt
     2821 cgcggttatt cgcgtgacgt actgacttat cgagggctga caatggcctt gcttatcggt
     2881 atcgtcattg gttatatcgt ggcgcagttt atttaatgat tcttccctgg atatatcttg
     2941 tttttaaagc ataactgttt gtttatgctt tgtttttttg ataactcacg tataatgcgc
     3001 gccttcgcgc aataaacacc gcatgactgc cttgaacaca ctattctctg acatttctcc
     3061 tttcactggc atggtgctgg cctgcctgct tggaggagtc ttgagcgttt gtgtcgctgc
     3121 tttgtttgcg ctcaatgcca aaaaacattg ggtccctttg ctggtgagtt atgcgattgg
     3181 tgctttgctg gcagccgtat tccttgagat tttgccgcat gcaattgaaa ccagtcctaa
     3241 catagaggcg atgaccgcca ccatgctgtt tggcgtactg ctatttttta cactggaaaa
     3301 gctggtgtta tggcgccatt gccacggtga acactgtgaa atgcatgcca tgcatgatga
     3361 ggcgcattgc cctgaattac atcctgagca gcagggcagc aagaagccac aaggtgcgat
     3421 caaattcaag ttgccacaag ctcagccagc gcaggtcgtc ggacatcatc atgcccatgc
     3481 ccacgatggc ggacgtagtg gcttgatgat tatggtgggt gataccttcc acaattttgt
     3541 cgacggtatc ctgatttgtg cagcgtttat ggtcgatatg caagtcgggt tggtgactgc
     3601 actggcgatt attgcgcatg aaattccaca agaggtgggt gatttcctga ttttgctgca
     3661 ttccggctac agcaagaaaa aagcattctt gtttaatatt gcatctagtc tggccacact
     3721 ggcgggtggg ctgattgctt atctgggact taatctggtc gagcattggg tgccgtatat
     3781 tttgagcctg gccgcagcga gtatgttgta tgtggcggtg gccgacttga ttcctgggtt
     3841 gcataaacgt acggcgttac gcgatacctt gagtcagtta ttattaattg tgttaggcgt
     3901 cgcttctgtc gccgtcacca agtggttggt agaaggttaa gagagagtcg ttatgcaaag
     3961 ttcgcctaaa gtcggttttg tctcgctggg ttgcccgaaa gcagggtctg actccgagcg
     4021 cattttgacc cagttgcgtg ccgagggcta tgaaatcagt aatagctacg agcagtctga
     4081 cctggtggtg gtcaatacct gtggctttat tgattcggcg gtgcaggagt cactggatgc
     4141 aattggcgaa gcgctcaata agaatggcaa ggtcatcgtc actggttgtc tgggcgctaa
     4201 aaaggatgtc gtgacaggtg cacatcccag tgtactcgct gtaacaggac cacatgcgct
     4261 ggaagaagtc atggggcatg tacacacgca tttgcccaag ccgcatgatc catataccga
     4321 cctggtgccg ccgcaaggca tccgcctgac gcccaaacac tatgcttacc tcaagatttc
     4381 tgaaggctgt aaccaccgtt gtagcttctg cattattcct agcatgcgtg gtgacctcgt
     4441 cagccgcccg attggcgatg tcatgaatga agcggaaagc ctggtcaatg ccggcgtgtc
     4501 tgaaattctg gtgatctcac aggatacctc ggcttatggc gtggatatga aattccgtaa
     4561 gggtttctgg aatggtcgtc caatcaaaac gcatatgacc gagctgagca agtctctggg
     4621 cgagttgggc gtttgggtgc gtttgcatta cgtataccca tacccacatg tagatgatgt
     4681 cattccactg atggcagaag gcgcgatttt gccttacctg gacgtgccat tgcagcatgc
     4741 cagcccgcgt atcctcaaat cgatgaaacg acctgccagc agtgaaaaca atctggcacg
     4801 tatcaaagct tggcgtgata tttgtcctga gattacgatc cgcagtacat ttatcgttgg
     4861 cttccctggt gaaactgaag acgatttcaa catgctgctc gactttatgg aagaggccca
     4921 gctggaccgc gtcgggtgtt ttgcttactc agccgtagat ggcgcggctg caaatgcctt
     4981 gcctgatcaa gtggcagaag aggttaaaaa agaacgttta agccgtttta tggaagtgca
     5041 ggagcgtatc agtgctgcca aagtcgctgc aaaagtggga agcctgcaaa cagtactggt
     5101 ggacgagtta acagaagatg aagatggtaa cgtgattgcc attgcccgta ccaaaggcga
     5161 tgctccagaa attgatggga ttgtttacct gcaggatgcc gagggcctga atcctggcga
     5221 ttttgtcgat gtgcagattg tggattcgca aggccacgac ttgatcggtg cgccgcctga
     5281 gttttaattt ctatgtctaa taataaaaaa tgtcgctatc gcggcatttt ttattattag
     5341 tgtcgcttag acgctcacag ccggtcgttc aaaatcggcg cgttcggcaa aggcatctag
     5401 cgtatatggg cagcgcttaa gtttttgttg agttgatttg cgtttattgc cttgagataa
     5461 gtttacgtaa ggcgtctggc tcaggcgaac ataacctgat tgcaggagct tgctggcagt
     5521 agtctcaaac tgactcagcg ggttatccat gttttgtagg gtaatgccta gcttatgtac
     5581 ggcaatgaca tggtaaatcg tgctggtttg gccagatacg gccggtttgc cataaagctc
     5641 gccgacatga atctctatgt tggaggatga ggggtgcgac atgcctgcat catagcgtgt
     5701 ttgggggcgg gcttcaatca ggcaaagtgt cttttacatc aagctaggaa tagggcagga
     5761 gtgagcatga catgttaaaa tagcaggctt gagtgttcag agagtcatca tgtcacaagc
     5821 tgttaaaacc cgttttgcac caagccctac cggatatctt catattggtg gcgcgcgcac
     5881 tgccttgttt tcttgggctt atgctaagcg tcatggcggc aagtttatcc tacgtattga
     5941 agataccgat gttgctcgtt cgacgccaga agcggtacag gctattctgg atgggatggc
     6001 ctggttgcag ctggatcatg atgaaggccc gttttaccag atgcagcgca tggacactta
     6061 caaaaagatc atccagaaaa tgctggatga aggtcatgcc tactattgct attgcagcaa
     6121 agaagaactg gacgccttgc gagaaagtca gatgcagcaa ggcctgaagc cgcgttatga
     6181 tggccgctgg cgccctgagt ctggcaaggt cttgccagag ccacctaaag atgtgcctcc
     6241 agtggtacgc tttaaaaatc cgacagatgg cgtagtggcc tgggacgata tggtcaaagg
     6301 ccgtatcgag atcgccaacg ctgaactgga tgacctgatt attgcccgtg ccgatggtac
     6361 gcccacttat aacttctgcg tggtggttga tgattgggaa atgggggtga cgcaggtcat
     6421 ccgtggcgat gaccatgtga ataacacgcc gcgccagatc aatatgctgc aagccttggg
     6481 tgcgcaagtg ccgcaatatg cccatttgtc catgattctt ggcgatgatg gccaaaagtt
     6541 atccaagcgc catggcgccg tgagtgtgat gcaatatcat gaagatggtt acctgcgtga
     6601 ggcggtcctc aattacctgg cgcgcttagg ctggtcacat ggtgatgatg aagtattcag
     6661 catggagcag ttctgtgagt ggtttgacct cgaccatatc acgccatcgg cggcgcagtt
     6721 taataccgag aaactgaact ggcttaacgc gcattatatt aaaaccttgc cattggagcg
     6781 cattgctgac gaagtcaagc cgcgtctggc tgcgctaggc atcaccgaaa tacagtcgcc
     6841 ggacttgctg gcggtattaa atctgtatcg cgagcgtgcc aacaacctca atcagctggc
     6901 gaaagaaatt gcgttcttct accagatgcc tgcgatcaat gaagcagatg ccgttaaaca
     6961 catccaggaa gaaaccgctg cgtatatcga aagttttttg caaaagactc aagattcgga
     7021 ctgggctgcc gataaattac acgatgcgct aaatcaggtc gtggctgagc aacaaattaa
     7081 attccccaga ctggccatgc ctttaagg
//
LOCUS       DATMSK010000110         4315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_31873,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000110 DATMSK010000000
VERSION     DATMSK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31873"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..162
                     /locus_tag="VNZ84_03775"
     CDS             10..162
                     /locus_tag="VNZ84_03775"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HWU66105.1"
                     /translation="MLYSSTGLRFPFIFGLSASTQVDYNYDSRPVDDRVKGDTRALFT
                     LGYSWK"
     gene            309..1244
                     /gene="ylqF"
                     /locus_tag="VNZ84_03780"
     CDS             309..1244
                     /gene="ylqF"
                     /locus_tag="VNZ84_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HWU66106.1"
                     /translation="MAIQWFPGHMTSARKKAEETLEHIDVVIEVLDARVPAASHNPMI
                     EEMRNFRQRPNLKILNKADLADPQVTQAWLNYFNNQPNTKAVALSCKKPGDAKKIPGL
                     CRQLAPHRGTPLKPLRMMIMGIPNVGKSTLMNALLNRRAAKVGDEPAVTKSQQRFELS
                     DSLNITDTPGMMWPKIEHESDGYMLAASHAIGRNAVIDEDVAEFLGNLLLKQYPALMN
                     TRYKLDVMKLDVASIDGIDLLQAIAKRRSYKRTDGHWDMERTAMALLTDYRSGAIGRV
                     SLESPQSRAEMLALAAENMDKHASEAPTKAETDAL"
     gene            complement(1255..2445)
                     /locus_tag="VNZ84_03785"
     CDS             complement(1255..2445)
                     /locus_tag="VNZ84_03785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWU66107.1"
                     /translation="MLIFGAYQQRSLIMHWVRGLLVFALGLAAYYVSETSFIHGRQSV
                     YTAASVTIIALGISLFANGVRLLRGYRVYRYRTLLVVTISLAGNAVMLNTYMPDRWIV
                     LFNLGVLMWVFGHCTRSLVGHGKDFVAHVFWLCCSLFGVITLMLALLTVKVMLTDDNA
                     LHTFPDWSVHAYMTTAIMLVMLSVTSLLLLVMHARAQAGLQAIATLDGLTGVLNRRGL
                     QEAAARMQAVSQRLRLPMGMLMVDLDYFKKVNDVYGHLVGDVVLKSCAGTIRAALRGG
                     DVIGRYGGEEFCVLMPNTGEQEAMILAERIRRIIEVTPVSIAGVESMRSETQAIKCTV
                     SVGAVSSETVGYLLESLFSAADQNLYKAKQNGRNRIISNVGQSQPTPTEEGKPAKRHA
                     SLLA"
     gene            2647..>4315
                     /locus_tag="VNZ84_03790"
     CDS             2647..>4315
                     /locus_tag="VNZ84_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWU66108.1"
                     /translation="MQHLLNPPQREAIHYLDGPLLVLAGAGSGKTRVITQKIAYLINQ
                     AGYKANQIAAITFTNKAAREMQERVTPLLEGKSGKGLTVTTFHSLGLQILRQEAALLG
                     YKPQFSILDASDSFKILSDILATTDKQLLRKTQWQISSWKNAFVNPDQAKSQADEELT
                     HAAARVYQLYQQTLRAYQAVDFDDLIKVPVELFDQHSDMLDRWQRKLHYLLIDEYQDT
                     NACQYKLIKQLTGLRGQFTAVGDDDQAIYGWRGADVENLRQLTTDFSKLKVIKLEQNY
                     RSTVRILRAANQVIANNPKLFEKKLWSELGTGDLLHVTATQSEEHEAETVVMKLQAHK
                     FEHRTTYKDYAILYRSNHQARIIEKFLRNHKIPYTISGGSSFFDKAEIKDIISYLRLV
                     ANEDDDPAFIRAATTPKKGIGNTTLERLGEYASAHKMSLFAAAFEGGFQHDVGAKQLE
                     DLLTFCRYIQTLQGRAVKDPANEVLNDLLTAIQYETYLYDGEEARAAETRWGNVMEFV
                     AWLSKKGEGSTEYGNESDGKNLLELTQLVSLITMLEGREDSEPDAVKL"
BASE COUNT         1174 a         1209 c         1023 g          909 t
ORIGIN      
        1 tcccagatcc tgctttatag ctctactggt ttacgcttcc cctttatttt tggcttgagc
       61 gcgtcaaccc aggtcgacta taactatgat agccgcccgg tagatgatcg tgtgaaaggc
      121 gatacacgcg ccctatttac acttggctat agctggaagt gacgtcaaat tgcacaacat
      181 ttagccgcgg gtgccttaaa tgttaaaatg acggcttgtt tgcgtgagcg tgactgagtc
      241 tgcacgcaat acgggtacgg cacaggacat tgataccgta tttttgagtt tgaagatgga
      301 gcgaaaatat ggcgatacaa tggtttccgg ggcatatgac ctctgcccgt aaaaaggcag
      361 aggaaacact ggagcatatc gatgtcgtga ttgaggtact ggatgcgcgc gtccctgccg
      421 ccagccacaa cccgatgatt gaggaaatgc gcaacttccg ccagcgcccc aaccttaaaa
      481 ttctcaacaa ggcggatttg gccgacccac aagtcacgca ggcctggttg aattacttta
      541 acaaccaacc caataccaaa gctgtcgccc tctcctgcaa aaagcctggt gatgcaaaaa
      601 aaatccccgg cttgtgtcgc caactggcac ctcaccgcgg cacgccgcta aagccattgc
      661 gcatgatgat catgggcatt cccaacgtcg gcaagtccac cctgatgaac gctttgctta
      721 atcgccgggc agccaaggta ggtgatgaac cagccgtgac aaaaagtcag caacggtttg
      781 aactcagtga cagcctgaat atcacggata cgccaggcat gatgtggccg aagattgaac
      841 atgaatcaga tggttatatg ctggccgcca gccatgccat tggccgcaac gccgtgattg
      901 atgaagatgt ggccgagttt cttggcaatc tgttgttaaa acaatatcct gcgctcatga
      961 atacacgtta caagctggat gtcatgaagc tggacgttgc cagcatagac gggattgact
     1021 tgctacaagc catcgccaaa cgccgcagct acaaacgtac tgatggtcat tgggatatgg
     1081 agcgtacggc gatggccttg ctgactgact ataggagcgg agcgattggg cgggtaagcc
     1141 tggagtcccc gcagagccgc gctgagatgc tggcgcttgc tgcagaaaat atggacaagc
     1201 acgcgagtga agcaccaaca aaagcagaaa ctgatgcgct ataagtgatt caggctacgc
     1261 cagtaagctg gcatgccgtt ttgcaggctt cccctcttcc gtcggggtag gctgtgactg
     1321 gccaacattg ctaataatac ggttacggcc attttgcttg gctttataca ggttttgatc
     1381 ggcagccgag aacaagctct ccagcaggta gccaacggtt tctgaactca ccgcaccgac
     1441 actgaccgta cacttgatgg cttgtgtctc gctacgcata ctttccacgc cagcaatgct
     1501 aaccggggtc acctcaataa tacggcggat acgctccgcc aatatcatcg cctcttgttc
     1561 gccagtattt ggcatcagga cacaaaactc ctcaccgcca tagcgcccaa ttacatcccc
     1621 acctcttaat gcggcacgta tggtgcccgc acaagacttg agcaccacat ctccgaccag
     1681 atgcccatac acatcattca ctttcttgaa gtaatccagg tctaccatca gcatccccat
     1741 gggtaaccgg agccgttgcg aaaccgcctg catacgtgca gccgcctctt gcaaacctct
     1801 gcggttcaac acgccggtca ggccatccag cgtcgcaatg gcttgcaggc cagcttgagc
     1861 acgcgcatgc atcaccagta acaacaaact ggtcactgac agcatcacca gcatgatggc
     1921 tgtcgtcata taggcatgca cagaccaatc cgggaatgtg tgcaaggcat tgtcatccgt
     1981 taacatcact tttaccgtaa gcaatgccaa catcaaagtg atcacgccaa acaggctgca
     2041 gcacaaccag aacacatgcg ccacaaagtc tttaccgtgc ccgaccaaac tgcgtgtgca
     2101 gtggccaaat acccacatta aaacgcctag attgaagagt actatccatc tgtctggcat
     2161 ataggtattc agcatcaccg cattgccggc gagtgaaatg gtcactacta acaacgtgcg
     2221 gtagcgataa acgcgatacc ctctaagcaa gcgtacgccg ttggcaaaca ggctgatgcc
     2281 aagtgcaatg attgtgactg atgccgcagt gtaaacactc tgccgcccat gtataaagga
     2341 agtttctgaa acgtagtacg cagccagccc caaagcaaaa actagcaatc cgcgcaccca
     2401 atgcatgatc aggctgcgtt gctgataggc gccaaaaatc agcaagacca gaacctgcgt
     2461 taaggtaagt ccaattgatg atagaatgac actgttgaga tcaaacacag ggacgtacaa
     2521 agtttgttta cctgagaaac atattactat ggttgagcga ctatacccat ataaatgaag
     2581 gggttaaata gtccatacag gctaattcac caacgctttg ttgtttacca cataccctga
     2641 agtcccatgc agcatcttct aaatcctccg caacgcgaag ctatccatta cctagacggt
     2701 cctttactcg tattggctgg cgccggcagt ggtaaaacgc gggtgattac acaaaaaatt
     2761 gcctacctga tcaaccaggc cggttataaa gccaaccaaa tcgcggcgat cacctttacc
     2821 aataaagccg cccgcgaaat gcaggagcgt gtcacaccgc tacttgaggg caaaagtggt
     2881 aaaggcttaa ctgtcaccac ctttcactcg cttggcctgc aaatcctgcg ccaggaagct
     2941 gccctgctcg gctataaacc tcagttctcc atcctggatg cttcggacag tttcaaaatc
     3001 ctcagcgaca ttttggcaac cacagacaaa cagttgctac gcaaaaccca gtggcagatt
     3061 tccagctgga aaaacgcgtt tgtgaacccc gatcaagcca aatcacaagc ggatgaagag
     3121 ctcacacatg ccgctgcccg cgtttaccag ctttaccagc aaaccttgcg cgcctaccag
     3181 gcagttgatt tcgacgatct gatcaaagtc ccggtagaac tgtttgatca acatagcgac
     3241 atgctggacc gatggcagcg caaactgcac tacctgctga ttgacgagta ccaggatacc
     3301 aacgcttgcc agtacaaact gattaagcaa ctcaccggcc tgcgcggcca gtttaccgcc
     3361 gtaggtgatg atgaccaggc catttacggc tggcgtggcg ccgacgtgga aaacctgcgc
     3421 cagctcacca cagacttttc aaaactcaaa gtgatcaagc tggagcagaa ctaccgctcc
     3481 accgtgcgca ttttgcgtgc agcaaaccag gtcatcgcca acaatcccaa gttgtttgag
     3541 aaaaaactgt ggagtgaact gggtaccggc gacctgctgc atgtcaccgc cacccaaagc
     3601 gaagagcacg aagcagaaac cgtggttatg aagttgcagg cgcacaaatt tgagcatcgc
     3661 acgacctaca aagattacgc gatcctctac cgcagcaacc accaggcgcg cattattgaa
     3721 aaatttttgc gcaaccataa aatcccctac accatctctg gtggctcctc gtttttcgac
     3781 aaagccgaaa tcaaggacat catcagctac ctgcgcctgg tcgccaatga agatgatgat
     3841 ccagcgttta tccgcgccgc caccacgccg aaaaaaggca tcggcaacac cacactggag
     3901 cgcctgggtg aatatgccag tgcacacaaa atgtcgctgt tcgccgccgc atttgaaggc
     3961 ggctttcagc acgacgtcgg tgccaaacag ctcgaagacc tgctcacctt ctgccgttat
     4021 atccagacat tgcagggccg cgccgttaag gacccagcca acgaagtgct caatgacctg
     4081 ctaaccgcca ttcaatacga aacctatttg tacgatggcg aagaagcacg cgctgccgaa
     4141 acacgctggg gcaacgtcat ggagtttgtc gcctggctca gcaaaaaagg cgaaggctct
     4201 acggaatacg gcaatgaatc tgacggtaaa aacctactcg aactcacgca actggtctca
     4261 ctcatcacca tgctagaggg ccgcgaagac agcgagccag atgccgtcaa actct
//
LOCUS       DATMSK010000111        10898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_266956,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000111 DATMSK010000000
VERSION     DATMSK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266956"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..925)
                     /gene="soxZ"
                     /locus_tag="VNZ84_03795"
     CDS             complement(131..925)
                     /gene="soxZ"
                     /locus_tag="VNZ84_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HWU66109.1"
                     /translation="MWLKAGLALVAMPFWLVSRKLEAAPWFAQAFEAKQTDQVMAVLK
                     ATAVPVTPELQIEAPQKAENGAVVQVELTSHQPAGSVSSLRVLADANPTPLIASFEFG
                     KQVLPKVVTRIKLAQSGEVIALLQQVSGQFQQQRREVIVLEDGCAGSEREEPFATSMK
                     MRARILEGGALGKNITELKIIILHPMRTGRSKNDDGQLMPAHFMQLMQVVLNGQVVVD
                     AQTGTGISRNPYFTFYLKDAKAGDVIAVNWQDNLGYEGHGQVTVSA"
     gene            complement(957..2522)
                     /locus_tag="VNZ84_03800"
     CDS             complement(957..2522)
                     /locus_tag="VNZ84_03800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWU66110.1"
                     /translation="MALNYIKHAYHSRRQRALRWLLVCGLLVVPAVALQANNATGIFS
                     GKPYEPDLDLSLPADSSNQLPELGDASQTVLSLRDEEKIAHQILKQVATSEDVLDDAE
                     VTDYLQALGSRLGESSPDKQQKFYFFVVQDKTINAFAMPGGVIGVHTGLFMAANSESE
                     LASVLGHEIGHVTQHHLARMLASQKYDMFKNLAATALALLIARSNPQVGMGAMTAASA
                     AGVQKQLDYTREHEREADRVGLTILQDAGFDVRAMPAFFTTMQRATRFSDGGSLPSYL
                     RTHPLTSERIADIGNRVQNLPYKQINESLAFFLMRAKIRAYQGAPSQMVEEFQNNISE
                     GRFQNEIAEHYGLAYAWLRSNQAKNAQVEVDWLVQHGMNNPYLVTLKGLTLAKLSQVA
                     QAQTQFVQGLQQYPKHRILAQGEAELLIRSNQWPQAVTFIQKQLETFPDDPKLWDWLA
                     AAYQGQGKQMLRHQALGESYVRKYDLPRAIDQFDIASKAKDGDFYLQSKVEARLKILR
                     QQLKLEQGDDKNG"
     gene            2634..3125
                     /gene="moaC"
                     /locus_tag="VNZ84_03805"
     CDS             2634..3125
                     /gene="moaC"
                     /locus_tag="VNZ84_03805"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWU66111.1"
                     /translation="MTKQISGLTHFNAQGHAHMVDIGDKAHTKRIAIAEGRIEMQAAT
                     LQTILQGNAKKGDVLGIARIAAIQASKKTSDLIPLCHPLSLSRVDVSFMADETQYSIT
                     CRVQCETTGQTGVEMEALTAVQVGLLTIYDMCKAIDRGMTIQGVRVLEKHGGKSGDWV
                     AND"
     gene            complement(3842..7630)
                     /gene="metH"
                     /locus_tag="VNZ84_03810"
     CDS             complement(3842..7630)
                     /gene="metH"
                     /locus_tag="VNZ84_03810"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWU66112.1"
                     /translation="MTQLPISATEQWLREALSQRILILDGAMGTMIQQYKLTEADYRG
                     ARFKDFQGPPGERELFVKGNNELLTLTQPHVIQEIHEKYLAAGADIIETNTFGATSVA
                     QDDYHMAHLVYEMNVEAAKLAKAACAKYSTPDKPRFVAGALGPTPKTASISPDVNDPA
                     ARNVSFDQLVASYHEQIRGLVDGGADILMVETIFDTLNCKAALFAIETFFEEQGKTWP
                     IMISGTVTDASGRILSGQTVTAFWHSVRHVKPLTVGLNCALGATLMRPYAEELSKVAD
                     TFVCIYPNAGLPNPMSDTGFDETPDVTSSLVKEFAQSGFINIAGGCCGTTPPHINAIY
                     NAIKDLPPRQVPTLPVTTKLSGLEPFVIDEQSLFVNVGERTNVTGSKAFARMIINEQY
                     EEALSVARQQVENGAQVIDINMDEGMLDAVKAMTHFLNLVASEPDIARVPIMIDSSKW
                     AVIEAGLKCVQGKAIVNSISMKEGEAEFLRQAKLCQRYGAAVVVMAFDETGQADTFAR
                     KTEICKRAYDLLVGSGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPY
                     ARISGGVSNVSFSFRGNEPAREAIHTVFLYHAIQAGMTMGIVNAGMVGVYDDIPAELR
                     ERVEDVVLNRVVDPDNPLAATERMIEIAGTLVAGGKKAEATLAWRGTPEQPVPATKRL
                     EYALVHGITDFIVADTEEVRQDIMSKGGRPIHVIEGPLMDGMNVVGDLFGQGKMFLPQ
                     VVKSARVMKSAVAHLIPFIEEEKAAEERRTGMVAKPKGKVVIATVKGDVHDIGKNIVS
                     VVLQCNNFEVVNMGVMVPAAEILEMAKRENADIIGLSGLITPSLEEMAYIAKEMQRDP
                     HFVENQTPLLIGGATTSRAHTAVKIAPNYEGPVIYVPDASRSVSVMQNLLTPETRGAY
                     LAEIQADYEKARHQHANKKGVPMLSLADARANAAKLDFTGSNTPVKPKFIGRRVFKNI
                     DLALIAQYIDWGPFFQTWDLAGFYPAILKDEIVGEAATKVFAEAQVMLKRIIDGRWLS
                     ANGVIALLPANQVNADDIEIYTDDTRSQVALTWYGLRQQTQKPVIDGVARPNQCMADF
                     IAPKGTDDYIGLFAVTAGLGMEKRLAEYEKNHDDYNAIMFKSLADRLAEAFAEYMHER
                     VRKDLWGYVADEQLTNEALIKEQYKGIRPAPGYPACPDHTVKAEMFKALQAEEIGMQL
                     TDSYAMLPAAAVSGFYLAHPESRYFSVDKIGNDQLEDMAKRRNVDKAWLERWLAPNLG
                     "
     gene            7835..8221
                     /locus_tag="VNZ84_03815"
     CDS             7835..8221
                     /locus_tag="VNZ84_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HWU66113.1"
                     /translation="MISGLVQLFLWQGLGELLSKLFLPGIPGPVLGLLLLLAFLCLKG
                     QVNSDLGLVADSFRQHLGLLFVPASVGVLLFLPELQTHALAVGTALIVSVILTIAVTA
                     IVLKVFWYFSLKRSKGNTGLKGQRHE"
     gene            8214..8954
                     /locus_tag="VNZ84_03820"
     CDS             8214..8954
                     /locus_tag="VNZ84_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HWU66114.1"
                     /translation="MNKRIPISEIWVYLSGDPLFALIITLATYQLGYLLYVRAKRNPI
                     VNPVAISVILTCLAIDLLDMPYEKYFEGAQFVHFLLGTATVALAVPIYNGFKRMQGKI
                     FHIWVALTVGSTVSIISAVAIAKLMGATDNIVGSMYAKSVTAPIAMGIAERLNVSPTL
                     TAVFTVITGMLGAILAPYILNTIKVQQWWIRGTAIGVGADGLGITRAFSVNEEAGVFA
                     SMAMGLNGVISAIGLPIVLTYLRSHLGF"
     gene            9205..9540
                     /locus_tag="VNZ84_03825"
     CDS             9205..9540
                     /locus_tag="VNZ84_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66115.1"
                     /translation="MQKHLNTAALQLRLWVVTVLLAVLGFALVAEFSDHSGYSDGTAR
                     VRIAEVVEYSGDGDSPLTSLIPLISPPASMTIACVIPFALLALPRRRVTPRPVFVRHL
                     HSRAPPSLP"
     gene            9778..10860
                     /locus_tag="VNZ84_03830"
     CDS             9778..10860
                     /locus_tag="VNZ84_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU66116.1"
                     /translation="MIKQNLIVLGLLGSLLSACHHEEAHEEAPPKLEVTHPWRKDAMI
                     TKEYVCQIHAIQHIELRALERGYLQDVFAGEGQIVKQGQPMFKIMPNVYQVDLLKAQA
                     EAKTVQIEYENTKALAEKNIVSQNELALGKAKLDKANAEVQLAQTHLNFTNINAPFTG
                     IMDHLLVRKGSLLDEGDLLTTLSDNSRMWVYFNVPEAEYLDFKIHQKNEKGVPVKLRM
                     ANGALFDQKGAIETVEADFDNKTGNIEFRADFPNPNGLLRHGETGTILLEVPHPNALQ
                     IPQKATFEVLDKTFVYVINKDHKVEQRMIQIAAELPHLFIIKSGLKETDTVLLEGLRR
                     VKNGQKIELDYRAPEKVIPELELYAE"
BASE COUNT         2611 a         2955 c         2692 g         2640 t
ORIGIN      
        1 gggttaatta aaaatttgct gcgtttccac agcgaatgga tgcattttaa gcatacacat
       61 ttaatatgta aaattgatta ttttaatttt attgataaat attactaatc aaaaagccat
      121 ctgccaaaaa tcaggcagaa acggtgacct gcccatgccc ctcataaccc aggttatcct
      181 gccagtttac cgcaatcaca tcacccgcct tggcatcttt gaggtaaaag gtgaaataag
      241 ggttacgcga aatgccggta ccggtttgcg catcaacaac cacttgtcca ttgagcacca
      301 cctgcatcaa ctgcatgaaa tgtgcaggca tcaactggcc atcatcgttt ttactgcggc
      361 cagtacgcat gggatgcaga ataatgatct ttagttcagt gatgtttttg cccaaggcgc
      421 caccttccag tatccgtgcg cgcatcttca tactggtagc aaacggctct tcacgctcgc
      481 taccggcaca accatcctcc agcacaatca cctcacggcg ttgttgctga aactggccgc
      541 tcacctgctg cagcaaggca atcacttcgc ctgattgcgc cagcttgata cgtgtgacca
      601 ctttgggcag tacttgttta ccaaattcaa agctggcaat cagcggcgtc ggatttgcat
      661 ctgccagcac acgtaaactg ctcacactgc ctgcaggctg atgactggtc agttcgactt
      721 gtaccaccgc gccattttca gctttttgcg gcgcttcaat ctgcaactca ggtgtcaccg
      781 ggaccgcagt ggctttgagc actgccatca cttgatcagt ttgcttggcc tcaaaggctt
      841 gcgcaaacca gggagcagcc tccagcttgc gcgagaccag ccagaatggc atggccacca
      901 acgccaggcc cgcctttaac cacactcttc gttgcttcca tggagcccga aggccatcat
      961 ccatttttat catcgccttg ctccagctta agttgctgcc tgagtatttt gagccttgcc
     1021 tctactttgg attgcaaata gaaatcacca tccttcgctt tgctggcgat atcaaactgg
     1081 tcaatcgccc gcggtaaatc atatttgcga acatagcttt cacccagcgc ctgatgccgc
     1141 agcatctgct tgccctgccc ctggtaagct gctgccagcc agtcccaaag cttggggtca
     1201 tccggaaaag tttccaactg cttttgaata aaggttacgg cctgcggcca ctggttgcta
     1261 cggatcagga gctcagcctc cccctgggcc aaaatgcggt gtttggggta ttgctgtaat
     1321 ccttgcacaa actgagtttg cgcttgggcc acctggctca acttagctag cgtcaacccc
     1381 tttagcgtca ccaggtaagg attattcatc ccatgttgca ccagccagtc tacctccacc
     1441 tgcgcattct tggcctggtt tgaccgtagc caggcatagg ccaaaccata gtgctcggcg
     1501 atttcattct gaaaacggcc ttcactgata ttgttctgga actcctccac catttgcgaa
     1561 ggcgcgcctt gatacgcacg gatttttgcc cgcatcagga aaaaagccag actctcattg
     1621 atctgcttgt aaggcaggtt ctgcacacgg ttaccaatat cggcgattcg ctcactggtc
     1681 agcgggtggg tacgtaaata gctgggcagg ctaccgccat ccgagaaacg tgttgcgcgc
     1741 tgcatggtgg taaaaaatgc cggcatggca cgtacatcaa acccggcatc ctgcaaaatg
     1801 gtcaggccga cccggtccgc ctcacgctca tgttcgcgtg tgtaatccag ttgcttctgc
     1861 acaccggcag ctgaagctgc ggtcatggca cccatgccca cctgtggatt cgaacgggca
     1921 atcagcaagg ccagtgctgt cgctgctagg ttcttaaaca tatcgtactt ttgtgacgcc
     1981 aacatacgcg ccaagtgatg ctgggtcaca tgaccaattt catgaccaag tacgctggcc
     2041 aactccgatt cactgttggc agccataaac aaaccggtat gcaccccgat cacgccacct
     2101 ggcatggcaa acgcattgat ggttttatcc tgcaccacaa aaaaataaaa cttttgctgc
     2161 ttatccgggc tactctcacc caagcgcgaa cccagtgcct gcaggtagtc ggtcacttcc
     2221 gcatcatcaa gcacatcctc gcttgtcgct acctgcttga ggatctggtg cgcaattttt
     2281 tcctcatcac gcagtgacaa cacggtttgg gaggcatccc ccagttcagg caactggttg
     2341 gaagagtcag caggcaaact caaatcaaga tcaggctcgt aaggtttacc ggaaaatatg
     2401 cccgtcgcat tattcgcctg taatgccaca gcgggaacga ccagcaagcc gcacaccagc
     2461 aaccagcgca atgcgcgttg gcgacgggag tgataagcat gtttaatata atttaaagcc
     2521 atgaatttta gtgtttgtca tgtgagccct gaccacaggt tcaacacaat agcccaaact
     2581 tgttatgata acgacttaat cgcttgagtt cacccttttc acgccaccac cctatgacca
     2641 aacaaatttc aggcctcact catttcaacg cacagggcca cgcccatatg gtcgatatcg
     2701 gcgacaaagc ccataccaaa cgtatcgcca ttgccgaagg ccgtatcgag atgcaagcag
     2761 ccaccttaca aaccatcctg caaggcaacg ccaaaaaagg cgacgtgcta ggcatcgccc
     2821 gcattgccgc gatccaagct agcaaaaaaa ccagcgactt gattccactc tgccacccgc
     2881 ttagcctaag ccgcgtcgat gtcagtttta tggcagatga aacgcaatac agtattacct
     2941 gccgtgtgca atgtgaaacg actgggcaaa ccggggtaga aatggaagcg ttaacagctg
     3001 tgcaggttgg cctgctgaca atttatgata tgtgtaaagc gattgataga gggatgacca
     3061 tacaaggggt gagagtgctg gagaagcatg gtgggaagag tggggattgg gtggctaacg
     3121 attagttcaa acagttgaag ctttgagtat ttgaataaaa aaatatttta ggaaaaatcg
     3181 agatccttat ttcgccactt ggcgagtcac ttttctttgc ttgccccaca gaaagtaacc
     3241 aaagaagacg gcacccagcc atcacggcct tcggctgcct tgcgttgctc aacaaaataa
     3301 gccggtcacg aaactcatcc tggcagctca catactacgt gagctgctgc gggattcgga
     3361 cagtcgctcc ctctactctt attttgtttc cgctactcag cgtgatggaa tgggatttat
     3421 ctcgccaaac tcacatcgta gaaatttggg ccgcttaaaa aatcaattct tccctttaaa
     3481 tcacaaatgg tggcactcgc atggttttcg ggatccccat ctgacgcgcc gagtagcgca
     3541 ggcaaaataa gatgaagcga gcggctgttc gaatcccgcg gtggactgcg tcttgtgcag
     3601 gtcactagga tgagttctgc gagcgcttat tttgacgagc aacgcaggaa acaagcggaa
     3661 gctggggtgc actttctttt gattacttgt tctttgtgca agcaaagaaa agtaactcgc
     3721 tgaaaagcga aaaaatcgct acatcacctt gccaaaacac aaacatcaga ttcactttcc
     3781 ccatgcatca aaaaaaaggc gtacctctca gtacaccctt ctagctcaat cgaacaaacc
     3841 actaccctag attcggcgct aaccaccgct ccaaccaagc cttatccaca ttgcgtctct
     3901 tcgccatgtc ttccaactgg tcattaccaa tcttatccac actgaaatac ctggattcag
     3961 gatgcgccag gtaaaaacca ctcactgccg cagcaggtaa catggcatag ctatcagtca
     4021 attgcatgcc gatttcttcc gcctgcaagg ccttgaacat ctctgctttc acggtatggt
     4081 ccgggcaggc tggatagcct ggcgcgggac gaatcccttt gtattgctct ttaatcagtg
     4141 cctcattagt cagctgttca tctgctacat aaccccacaa atctttacgc acacgctcgt
     4201 gcatgtattc ggcaaaggct tcagctaaac gatcggccag tgacttgaac atgatggcgt
     4261 tatagtcatc gtggttcttt tcatactcgg ccaggcgttt ctccatgcct aaaccagccg
     4321 tgacagcaaa caggccaatg taatcatccg tgcctttagg ggcaataaaa tcggccatac
     4381 actggttagg gcgggcaacg ccatcaatga ccggtttctg cgtttgctga cgcaggccgt
     4441 accaggtcag tgcgacttga ctacgggtat cgtcggtata aatctcgatg tcatccgcat
     4501 tgacctgatt ggcgggtaac agggcgatga cgccattggc agataaccag cggccatcga
     4561 taatgcgctt gagcatgacc tgtgcttcgg caaatacttt agtggcagct tcgccgacga
     4621 tttcatcttt caaaatcgca ggataaaaac cggcgagatc ccaggtctgg aagaacgggc
     4681 cccagtcgat gtattgtgca atcagggcga gatcgatgtt tttgaatacg cgacggccga
     4741 taaacttggg tttgactggc gtgttgctgc cagtgaagtc gagttttgcc gcattggctc
     4801 gtgcgtcagc caggctcagc atgggcacgc cttttttatt ggcatgctgg tgacgcgctt
     4861 tttcgtaatc tgcctgaatt tctgcaaggt aagcaccacg tgtttccggc gtcagtaagt
     4921 tttgcatcac actgaccgaa cgcgaagcat ccggcacgta aatcaccggg ccttcgtagt
     4981 taggtgcaat tttcactgca gtgtgggcac gggaagtcgt cgcgccgcca atcagtaatg
     5041 gcgtctggtt ttcaacaaaa tgcgggtcgc gctgcatttc tttggcgata taggccattt
     5101 cttcgagtga tggcgtaatc aggccgctta aaccaataat gtcggcgttt tcacgcttgg
     5161 ccatttccag gatttcagca gcgggcacca tgacgcccat attcacgact tcgaagttat
     5221 tgcactgcaa caccactgaa acgatgtttt taccgatatc gtgcacatcg cctttcaccg
     5281 tggcgatcac gaccttgcct ttaggcttgg cgaccatgcc ggtgcggcgt tcttccgccg
     5341 ctttttcttc ttcaataaac ggaatcaaat gcgccacggc agatttcatc acacgcgccg
     5401 atttcaccac ttgcggcaaa aacatcttgc cctggccaaa caagtcaccc accacgttca
     5461 tgccatccat gagcgggcct tcgatgacgt gaatcggacg accacccttg ctcataatat
     5521 cttggcgcac ttcttcagta tcagcgacga taaagtcggt gatgccatgc accagcgcat
     5581 attccaggcg cttggtagcg ggcaccggct gctctggcgt gccgcgccag gccagggtgg
     5641 cttctgcttt tttaccgcca gccaccagcg tcccggcgat ttcaatcatg cgctcggtcg
     5701 ctgccagcgg gttgtcaggg tccaccacac ggttgagcac cacgtcttcg acgcgctcac
     5761 gtagctcggc cggaatatcg tcatagacgc cgaccatacc ggcattcaca atccccatgg
     5821 tcatgccagc ctggatggcg tgatagagga acacggtatg aatggcctct cgtgccggtt
     5881 cgttaccacg gaagctgaag ctgacgttgg acaccccgcc ggaaatgcgg gcgtatggca
     5941 ggttctgctt gatccagcgc gtcgcttcga taaagtctac cgcgtagttg ttatgctctt
     6001 cgatccctgt cgccacggca aaaatattcg ggtcaaagat gatgtcttct ggtgggaatc
     6061 cggagccgac caataaatcg taagcacgtt tgcaaatctc ggttttacgc gcaaaagtat
     6121 ccgcttggcc ggtttcgtca aacgccatca ccaccacggc cgcaccataa cgctggcata
     6181 atttggcttg gcgcaaaaac tcggcttcgc cttctttcat ggaaatcgag ttcacgatgg
     6241 ccttaccctg cacacacttc aagcctgcct cgatcaccgc ccatttggag gagtcgatca
     6301 tgatgggcac gcgtgcaata tcaggctcgg aggcgaccaa attgaggaaa tgcgtcatgg
     6361 ctttgaccgc atccagcatg ccttcatcca tgttgatgtc gatgacctgg gcgccgtttt
     6421 cgacctgctg acgcgccacg ctgagcgctt cttcgtattg ctcgttgatg atcatccgcg
     6481 caaacgcttt tgaacccgtg acgttggtgc gctcgccgac gttgacgaac agcgactgct
     6541 catcaatcac aaatggctct agcccggaca acttggtggt cacaggcaag gtgggtactt
     6601 ggcggggtgg taaatcttta atcgcgttat aaatggcgtt gatatggggc ggcgtagtcc
     6661 cgcaacagcc gccggcaata ttgataaagc cgctttgcgc gaactctttg accaggctgg
     6721 aagtgacatc tggtgtttcg tcaaacccgg tatccgacat cgggtttggc aacccagcgt
     6781 tggggtaaat acaaacaaag gtatctgcga ctttactcaa ctcttccgca tatgggcgca
     6841 tcaaagtcgc acccagggcg cagttcagac ccacagtgag cggtttgaca tggcgtacag
     6901 aatgccagaa ggcagtcacg gtctggccag agaggatacg gccggacgcg tcggtcaccg
     6961 tgccggaaat catgatcggc caggttttgc cctgctcttc aaagaaggtc tcaatcgcaa
     7021 acagcgcggc cttacagtta agcgtatcga agatggtttc caccatcagg atatcggcac
     7081 cgccatcgac caggccgcgg atttgctcat ggtaagaagc caccaactgg tcaaaactaa
     7141 cgttacgcgc cgctgggtca ttcacatcag gtgaaatcga cgcagttttt ggtgttgggc
     7201 ctaatgcgcc agcgacaaag cgcggcttgt ctggcgtgct gtatttggca caagcggcct
     7261 tggccagttt ggcggcctcg acgttcatct catacaccag gtgtgccatg tggtaatcgt
     7321 cttgcgcgac gctggtggca ccaaaggtat tggtttcgat gatgtcggcc ccggcggcca
     7381 ggtatttttc gtgtatctcc tgaatgacgt gcggttgcgt caatgtcagc aactcgttat
     7441 tacctttgac aaacaattcg cgctcaccgg gcggaccctg aaagtccttg aaacgcgcgc
     7501 cgcggtaatc tgcctcggtc agcttgtact gctgaatcat ggtgcccatt gcgccatcca
     7561 ggatcaggat gcgttgtgac agggcttcgc gtaaccattg ttcagtcgcg gaaatcggca
     7621 gttgcgtcat gcgtatcaac ttgaaaataa aagtgttatt ttatcacaag ctacctggca
     7681 gcccgccacc actggcaggc gtgctcaccc gctcgtgcca caaaaaaaca taggcgaatc
     7741 ggaaaaatga cattaccatc gcggcattct gtacatttac tcagggcggc cacggaatgg
     7801 tggtgtgtca cgcccggaca attgaggaaa gcgcatgata tcgggtttgg ttcaactctt
     7861 tttgtggcag ggcctgggtg aattgctcag caaactcttt ttgcccggca ttccagggcc
     7921 agtgctggga ttactgctat tgctggcatt tttgtgcctc aaagggcaag tcaatagtga
     7981 cctgggattg gtggctgaca gcttccgcca gcacctgggg ttattgtttg tgccggcttc
     8041 tgtgggtgtg ctactgtttt tacccgagtt acaaacccat gcgctggccg tgggcaccgc
     8101 actgatcgtc agtgtgatcc tgaccattgc cgtcacggcc atcgtactca aggtgttctg
     8161 gtatttttcg cttaaacgca gcaaaggcaa caccggcttg aaggggcagc gccatgaata
     8221 aacgcatccc gatttcagaa atttgggttt acctttccgg cgaccccttg tttgcgctga
     8281 ttattacgct ggcgacttac cagctgggtt acttgctgta cgttcgcgcc aagcgcaatc
     8341 ctatcgtcaa cccggttgcc atttcagtca tcctgacctg cctggccata gacctgctgg
     8401 acatgcccta tgaaaagtat tttgaaggtg cacagtttgt gcacttctta ctggggacag
     8461 caaccgtcgc actggcggtg ccgatttata acggcttcaa gcgcatgcaa ggcaagattt
     8521 tccatatctg ggtcgcgctc actgtcggca gcaccgtgtc tattatcagc gcagtcgcta
     8581 tcgccaaact gatgggggcc acggataaca tagtcggcag catgtacgcc aagtccgtca
     8641 ccgccccgat tgccatgggc attgccgagc ggctcaatgt gtccccgacg ttaacggccg
     8701 tgtttacggt gatcaccggc atgctggggg caattctggc accctatatt ctcaatacta
     8761 tcaaagtcca acaatggtgg atacgcggca ctgcgatcgg tgtgggcgcg gatgggctgg
     8821 ggattacacg ggcatttagt gtcaatgaag aggccggcgt gtttgccagc atggccatgg
     8881 gcctcaatgg tgtgatcagt gccattgggc tccctatcgt gctgacctat ttacgctcac
     8941 acctggggtt ctaaagagat cggcaatcgc gccgttaatt gctctgtaag ggggcaaatg
     9001 aacgcaccaa cttggtgcac ctgaaactga cacccagctt acaaacggcg cttttcttcg
     9061 aacttgggat aaaagcgtat tgtcattcgc tatgcataag ggtacattgt aagcaatgta
     9121 aagaggttta tctctgcttg gctaggattc actctctgcc gatttttaat actggctttt
     9181 tcactttttt catacactga tcaaatgcaa aaacacctga acaccgccgc attgcagctc
     9241 agactgtggg tcgtcacagt gctgttggct gtgctcggct ttgcattggt cgcagagttc
     9301 tcagaccact caggctatag cgatggcacc gcccgtgtcc gcattgccga agtggttgaa
     9361 tacagcggtg atggcgattc gccactcacc tcactgatcc cactgatttc acctcccgca
     9421 agcatgacca ttgcctgcgt gattcccttt gcattgctgg cgctacctcg ccgccgcgtg
     9481 acaccacgcc cggtgtttgt tcgccacctg cattcgcgag cacctccctc cctgccatag
     9541 gtgtcaaccg cgctagtaca ttgcatggcg ctactaaaca ccgacctgat tttccctaac
     9601 cgcaatttac agccctaccc gcgctgtccc ttcaggactg tgcccatgcc aacggtagta
     9661 gtttgcagta agataaggat agtctggcga ttcagagtag ccacaatata gcaagacatg
     9721 cgccgacacc ggatttgtgc aagtaacttt cttcattttt aagataagaa cacacttatg
     9781 attaaacaga acttaatcgt tttaggctta ctaggctcct tgctcagcgc ttgtcatcac
     9841 gaagaagccc atgaagaggc gccaccaaag ctggaagtaa cacatccatg gcgtaaagac
     9901 gccatgatta ccaaggaata tgtctgccag attcatgcga tccagcatat tgaattacgc
     9961 gccctggagc gcggttacct gcaagacgta tttgccggtg agggccagat cgtgaagcag
    10021 ggccagccca tgttcaaaat catgcctaat gtttaccagg tcgacttgct gaaagctcag
    10081 gcggaagcga aaacggtgca gattgagtac gaaaacacca aagccctggc ggagaaaaac
    10141 attgtttccc aaaatgaact ggcactaggc aaagccaagc tggataaagc caatgccgag
    10201 gtgcagctgg cgcaaacaca cctgaacttt accaacatca atgcaccgtt taccggcatc
    10261 atggaccatt tgctggtacg gaaaggcagc ctgctggatg agggagatct gctgacaacc
    10321 ctgtctgaca acagccgcat gtgggtgtac ttcaatgtgc cagaagctga gtatctggac
    10381 ttcaagatcc atcaaaaaaa tgaaaaaggt gtgccggtta aattgcgcat ggccaatggt
    10441 gccctttttg atcagaaagg tgccattgaa acggtagaag ccgattttga caataaaacc
    10501 ggcaatatcg agttccgggc tgatttcccg aatccgaatg gcttactgcg tcatggtgaa
    10561 actggtacta ttttgctgga agtacctcac cccaatgccc tgcagattcc gcaaaaagct
    10621 acctttgaag tgctggacaa gacctttgtt tacgtgatca acaaggacca caaagtcgaa
    10681 cagcgcatga ttcagatcgc tgccgaactg ccacatctgt tcatcatcaa gtcgggcctc
    10741 aaagagactg acacggtgtt gctggaaggg ctgcgtcgcg tcaagaacgg gcaaaaaatc
    10801 gaactggatt acagagcccc tgaaaaagtc attccagagc tggaattgta cgcagaataa
    10861 ggcttaggcc gtcatcacac gaaagtgact taatccat
//
LOCUS       DATMSK010000112         5275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_360684,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000112 DATMSK010000000
VERSION     DATMSK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360684"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VNZ84_03835"
     CDS             <1..298
                     /locus_tag="VNZ84_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU66117.1"
                     /translation="YKQLSIKDIAEKLMLEPATITPVVKRLEAKKLTKRTRQKDDERV
                     VIVSLTEEGQALRSKLSEVQKRVACDTGLNSTAFENLNKTLNELTKSVTAKIPD"
     gene            complement(427..654)
                     /locus_tag="VNZ84_03840"
     CDS             complement(427..654)
                     /locus_tag="VNZ84_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66118.1"
                     /translation="MCKKILLSGRAGFRVHYCETHRTIELEIGAMSLRLDEDALELMS
                     DALDESVTKLQALHATKGSFRAFMRQLNMPD"
     gene            complement(693..962)
                     /locus_tag="VNZ84_03845"
     CDS             complement(693..962)
                     /locus_tag="VNZ84_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66119.1"
                     /translation="MNTMNIHAIRYDPSLPDAESVMASLLYVATLYIKKPTYDLAKQA
                     LRLAETLTAPEYADSELICSVSRRMCVQWTLLVNEHEQAAIQLRA"
     gene            complement(977..1186)
                     /locus_tag="VNZ84_03850"
     CDS             complement(977..1186)
                     /locus_tag="VNZ84_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66120.1"
                     /translation="MKRHTVVGKTMLAGKTACKVLYHQASDMVELEVGGTTLKFEAGN
                     FIVINEMLRKAAARIVMQTEIEMTV"
     gene            complement(1312..3945)
                     /locus_tag="VNZ84_03855"
     CDS             complement(1312..3945)
                     /locus_tag="VNZ84_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU66121.1"
                     /translation="MSAQQLTSAGRGKLISYLWLMGLCALLICLVVLTYADLSASARH
                     ITILLLVIGIGLWCGVIGLAWYQQRAVLAKQVAELKTFQQALEHAYSDEGFVPVQFSP
                     AEEESVLTAWRQLVIQAESLGSQGKSGKQHHQQLNQLYLALSQCNQAIVQSQDKYSLF
                     EQICRIIVNLGHANMAWIGELGADNKRITILNAFGSGVNYLNEVEISADPAVPSGKGP
                     CGLSLMSDHPYWVHDFQTNPISAFWHEKANEFAWKSAAFLPLHVEGKVIGCLSLYADH
                     EHAFDQDTQRLLQGMAANIDFALDRFEVQAQRSLIQSHLRESEQRAQLVLENALDAIV
                     NIDMQGLIVEWNLAASHIFGYKRSEVLGRRLEDVIVPPEYWQAHQQGMLRLQQTGKSR
                     LLGKLIEITALRRDGTEFPIELTIVEIERDQEHYYSAFIRDITKRKEAEERIRILANY
                     DALTGLPNRNLLNERVTVEIEKAWDQDGQFALMFLDLDHFKDVNDSLGHRYGDNLLIE
                     LTKRFESLVRPQDTICRLGGDEFVFLLAEAGHVIAKEVVERLLKAVEQPFVIDQYELT
                     ITASIGVAIYPDDGLDMESLQRNADVAMYRTKKESRNSYRFFSNHMQLQTARNLQLVN
                     ALRHAITSGQLAVYYQPQVALDTGRLLGVEALLRWKHPQLGFISPAEFIPLAESSGLI
                     ITIGSWVLEQATQQMHAWQQAGIHGIAVSVNLSSIQFRDPKLPALVKSTLEQYKVSPD
                     CLELELTEGVALENPEGAVAMMDALNKLGVRLSIDDFGTGYSSLSYLKKFKVYKLKVD
                     QTFVRDISTDDEDKAIVIAIIQLARSLGLKTIAEGVETPEQKAFLQAQGCDEMQGYLL
                     SKPMSSEDATAFLLSYTPPHA"
     gene            complement(4123..>5275)
                     /locus_tag="VNZ84_03860"
     CDS             complement(4123..>5275)
                     /locus_tag="VNZ84_03860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWU66122.1"
                     /translation="EFMNVQTRIDEALFHRLLSGASIPLLGSAFGGLLIAFSQLDSEK
                     SHLFLLWCGVVYAVLIFRLYCLRRCKRMLAKHGFNYALAYRYCLTLALSGLAWGALGF
                     LVKDAPPMTVIIVITAIQAMVMGGVVTMASCIPAFFAFSLPAMLPTVLMFSLSENLSS
                     YVMAVFSVIFYLLMIGIAYRFNQSLRQAARISFENEDLIGSLKEAYHKISVQNEELNH
                     VAHHDALTGLPNRKLLSLRLQQAMELAKLKKNATAILYLDLDGFKAVNDQFGHEAGDL
                     ALIEVAQRLSRVIRGSDTLARVGGDEFVVVLSDLPEQAKASVEMVAQKCLMVFEHEFQ
                     VYGNVCRLGTSIGLTLCNQGESIEQLLSAADKAMYQAKLLGCGQMSWAH"
BASE COUNT         1403 a         1460 c         1182 g         1230 t
ORIGIN      
        1 atacaagcaa ttatccatta aagatatcgc agaaaaatta atgctggaac ctgccaccat
       61 taccccagtg gtcaaacgtt tggaagcaaa aaaactaacg aaacgtacgc gccagaaaga
      121 tgatgaacgc gtggtcattg tcagcctgac agaagaaggc caggccctac gcagcaagtt
      181 atctgaagtg caaaaacgtg ttgcgtgcga caccggcttg aacagtactg cgttcgagaa
      241 cctgaataaa acgctgaatg aactgaccaa aagcgtgaca gcgaaaattc cagactaaaa
      301 cgtcacaggc gcatttgaat cagtttgaca tgtgcttggc ctgatgccaa aactaaatga
      361 ggagccgcag ctcctcattt agtttttact cttgccagat atttaagaaa cagcggctct
      421 tacgcattaa tcaggcatat tcaattgccg cataaaggcc cggaaagagc ctttggtcgc
      481 atgcagggcc tgtaacttgg tgactgattc gtccagtgcg tcactcatca actctaacgc
      541 atcctcatcc aggcgcaggc tcatggcacc aatctccagc tctatggtgc gatgcgtttc
      601 gcaataatgg acacggaacc cggcccggcc tgacaacaaa attttcttac acatgctttc
      661 ctcactcatc atcattcccc taacccatcg ccttatgcac gcaactgtat ggccgcctgc
      721 tcgtgctcat tgaccagcaa agtccattgc acacacatgc ggcgcgaaac actgcaaatc
      781 aactcagaat ctgcgtactc tggggcagtc agggtttcag ccaggcgtaa tgcctgcttg
      841 gccaggtcat aggttggttt tttaatatat aacgttgcca catagagcaa cgacgccatg
      901 actgactcgg cgtcaggcag gctgggatcg tagcggatgg catggatatt cattgtattc
      961 atggcagtct ttccttctaa acggtcattt cgatttcggt ttgcatcaca atacgcgcgg
     1021 cggccttacg cagcatttcg ttaatcacga taaagttgcc ggcctcaaac ttgagcgtgg
     1081 tgccgcccac ctccagctct accatgtctg aagcttgatg ataaagcacc ttgcaggcgg
     1141 tctttccggc caacattgtc ttgcctacca cggtgtgacg tttcatcatg actctccttt
     1201 gataaacctt aaataaatga taataattct catttacaat gtcaagagaa tttgaacagc
     1261 gattttggga aagaaagcac ccctctattg cgggtagctt gagtagtgaa tttaagcgtg
     1321 tggaggggta tatgaaagca ggaacgcagt tgcgtcttcg ctgctcatcg gtttgcttaa
     1381 caggtagccc tgcatttcgt cacaaccttg cgcttgcaag aatgctttct gctccggggt
     1441 ctcaaccccc tcggcaatgg tcttgagtcc caagctgcgt gccagctgga taatggcaat
     1501 gacaatcgct ttgtcttcat catcggtact gatatcacgc acaaaggtct ggtccacctt
     1561 aagcttgtag actttgaatt ttttcaggta gctcaaagaa gaatagccgg tgccaaaatc
     1621 gtcgatcgac aagcgtacgc ctaatttatt caacgcatcc atcatggcga cagcgccttc
     1681 cggattttcc agcgccacgc cttcggtcag ctcaagctcc aggcaatcag gcgaaacctt
     1741 atactgctcc aaagtagact tcaccagcgc aggcagttta gggtcccgga actggatgct
     1801 ggacaaattc acggatacgg caatgccatg aatgcctgcc tgctgccagg catgcatttg
     1861 ttgtgttgcc tgctctagta cccagctgcc tatcgtaata atcaaaccac tggattcagc
     1921 caatgggata aattctgccg gcgagataaa cccaagctgc ggatgcttcc agcgcaacaa
     1981 ggcctccaca cccagcaagc gccccgtatc cagcgccacc tgcggctggt aatacactgc
     2041 aagttgcccc gaagtaatcg cgtggcgcaa agcattcacc agttgcaggt tacgcgccgt
     2101 ctgcaactgc atatggttgc tgaaaaaccg gtagctgttg cggctttctt ttttggtacg
     2161 gtacattgcc acatccgcat tacgttgcag gctctccata tccaggccat catctgggta
     2221 aatggcgacc ccaattgaag ccgtgatcgt taactcatac tggtcgatga caaacggttg
     2281 ctcgaccgct ttgagcagac gctccaccac ttctttggcg atcacatggc cagcctctgc
     2341 cagcaagaac acgaactcat ccccgcctaa ccggcaaatc gtatcttgcg gcctcaccag
     2401 actttcaaac cgtttggtca attcaatcag caggttatcg ccatagcgat gccccagact
     2461 gtcattcacg tccttgaaat gatccaggtc gaggaacatc agtgcaaact ggccatcctg
     2521 gtcccaggcc ttttcaatct caaccgtgac ccgctcattc agtaaatttc ggttgggcag
     2581 accggtcaag gcatcataat ttgccagtat gcgaatacgt tcttcagcct ctttgcgctt
     2641 ggtaatatcc cgaataaatg cgctgtagta atgttcttga tcacgctcaa tctccacaat
     2701 ggtcaactca atgggaaact cagtaccatc gcggcgtaag gcggtaattt cgatgagctt
     2761 gcccagcaag cgtgatttac ctgtttgctg caagcgaagc atgccttgct gatgggcttg
     2821 ccagtattct ggcggcacaa tgacatcttc gagccgacgg cccaacactt cactacgctt
     2881 atacccaaaa atatgtgacg ctgccagatt ccactccaca atcaggccct gcatatcaat
     2941 attcactatg gcatccagcg cattttcaag caccagctgt gcgcgctgct cactttcgcg
     3001 caaatgactc tggatcaaac tgcgctgcgc ctgtacctca aagcggtcca gggcaaagtc
     3061 aatattcgcc gccatccctt gcagaaggcg ctgtgtgtcc tggtcaaaag cgtgctcatg
     3121 atcagcataa agagataagc agccgatgac cttaccttcg acatgcagag gtagaaaagc
     3181 ggccgatttc caggcgaatt cattcgcctt ttcgtgccaa aaagcactga tggggttggt
     3241 ctggaagtcg tgaacccagt agggatgatc ggacatcagg cttaatccac aaggcccttt
     3301 gccacttggg acagcaggat cggccgatat ctcaacttca ttcaggtagt taacaccaga
     3361 accaaacgcg ttgaggatgg tgatgcgttt attgtcggca cccaactcac caatccaggc
     3421 catgttggca tgcccaagat taacaataat gcggcaaatt tgctcgaaca ggctgtactt
     3481 gtcctggctt tgtacaatcg cctggttgca ctgactcaaa gccagataga gttggttcaa
     3541 ctgctggtga tgttgcttgc cggacttgcc ttggctacca agcgattcag cctgaatgac
     3601 cagttgccgc caggccgtca gtacgctctc ttcctctgca ggggaaaact gaacaggaac
     3661 gaacccctca tcggagtatg cgtgctctaa cgcctgctga aatgtcttta actctgccac
     3721 ctgttttgcc agcacagcac gctgttgata ccaggcaagc ccaatgacac cacaccacag
     3781 gccaatccca atcacaagca gaagaatcgt tatatgacgc gcagacgcag acaggtcggc
     3841 ataggtgagt accaccaggc aaattaacag cgcacacagg cccatcagcc acaaataact
     3901 gatcagcttg ccccgccctg cagatgtgag ttgttgtgca gacatggatg tttagcctaa
     3961 gcccccgtcc actactgtaa gggatgtgat tgaaatcaaa ccccaaaata tgcatacaaa
     4021 tgcccggtaa attagccgat agaggacacc cccttcacgg ccaccacctc ttgtaaagca
     4081 attgcaactc caatgaccat tcgccctaaa ggcgcaccgg cactaatgtg cccaggacat
     4141 ttgaccgcaa ccgagcaact tggcctggta catggcttta tctgcagcag acaataactg
     4201 ctcgatggac tccccctgat tgcacaaagt caatccgatg gaggtcccta gcctgcaaac
     4261 attaccgtaa acctggaact cgtgttcgaa taccatcagg catttttggg ccaccatctc
     4321 taccgaggct ttcgcctgtt caggcaaatc agacaacact acgacaaact cgtcgccccc
     4381 tacacgtgcc agcgtatcgc taccgcggat cacccggctt aaacgttgcg ccacctcaat
     4441 taatgccaaa tcacccgcct catgtccaaa ttggtcattc acagctttaa agccatccag
     4501 gtccaagtaa agaatcgctg ttgcattctt tttcagcttt gctagctcca ttgcctgctg
     4561 taaccgcagg gacagcaact tgcggttagg caaaccagta agcgcatcgt ggtgggcaac
     4621 atggttaagc tcttcgttct gtaccgaaat cttgtgatag gcctctttta aagagccaat
     4681 caaatcttcg ttttcaaaac tgatacgcgc agcctgccgt aatgactggt taaaccggta
     4741 ggcgatccca atcatcagca ggtaaaaaat cacactaaac accgccatca cataactcga
     4801 caagttttcg ctcaatgaaa acatcagtac ggttggcagc atggccggta aactaaaggc
     4861 aaaaaaagcc ggaatgcagg aagccatggt caccacgccg cccatcacca tggcctggat
     4921 cgccgtaatg acaataatca ccgtcatcgg tggggcatcc ttgactaaaa agcccagtgc
     4981 cccccatgcc aatcctgaaa gtgccaaagt caggcaatac ctgtaggcta acgcataatt
     5041 aaacccgtgc ttggcaagca tgcgcttaca gcgcctgaga caataaagcc tgaaaatgag
     5101 cacagcataa accacgccac accaaagcaa aaacaaatgg cttttctcag agtcaagttg
     5161 tgaaaacgcg atgagcaagc cgccaaacgc actcccaagt aaaggaatgc ttgctcccga
     5221 taacaacctg tgaaatagcg cctcgtcaat ccgagtttgc acattcatga attca
//
LOCUS       DATMSK010000113        12225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_8815, whole
            genome shotgun sequence.
ACCESSION   DATMSK010000113 DATMSK010000000
VERSION     DATMSK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8815"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..413
                     /locus_tag="VNZ84_03865"
     CDS             3..413
                     /locus_tag="VNZ84_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HWU66123.1"
                     /translation="MLGACASSNSGSVYSRDEARRAQTVKTGVVESVRPVKLEGTKSP
                     VGTVAGGAIGGIAGSSIGSGKGSAIAAVIGAVAGGLAGSAAEEVATRKNALEITVKLD
                     GGGLVAIVQEADEQFNAGERVRIVESGGTSRVSH"
     gene            562..1350
                     /locus_tag="VNZ84_03870"
     CDS             562..1350
                     /locus_tag="VNZ84_03870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HWU66124.1"
                     /translation="MLERNLQNLGTYQPDIQPSANSSRIPSTTLSVSLASSAEEIMEA
                     QRLRYQVFAEEMGAQIKSQNGLDVDGFDAFCDHLVVRDDATQQIVGTYRILNPMQAML
                     AGGYYSAGEFDLSRLAPLADRMVEVGRACVHADYRTGGTITMLWAGLAKYMMARQYEY
                     MIGCASISMHDGGHTAASLFNNLREDYLSPAEYRVFPHNPLPIEALDQSLPVTCPPLV
                     KGYLRLGAYICGEPAWDPAFNTADMLVLLPLSRINPRYAAHFLK"
     gene            1352..2152
                     /locus_tag="VNZ84_03875"
     CDS             1352..2152
                     /locus_tag="VNZ84_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWU66125.1"
                     /translation="MPKTNFLTRYWRIARIFLHTLTGLTLATLVLPVCGRRGREQIIR
                     WWCAKLLRCFNLQLSVSGQCPASQTQHTLFVANHISWSDIHALNSLIPVRFVAKLEIK
                     DWPVFGYLVKKSGTIFINRTRQRDAARVLKVVTQAMKLGDNLCVFPEGTTTDGQQVLP
                     FKSSLIQASIDAEATVIPIAIHYPLPDGSPNLAAAYAGETTMIESMLSFLNMQSPRVH
                     LHFCTPIEARLLDRQTLATQARNEIIAHLPWATTSEVVSQPSLQRQQA"
     gene            complement(2186..2944)
                     /locus_tag="VNZ84_03880"
     CDS             complement(2186..2944)
                     /locus_tag="VNZ84_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HWU66126.1"
                     /translation="MELWLVISAAFLAGIVDAMVGGGGLVTVPALLTTFPNVAPALLL
                     GTNKSSSVFGTAVAAWRYGRHVQLDWRWLAPAALAALIGSGLGAWTLTWTDPDFLRKL
                     LPWVLVLVLFYTFKYPQFGLSRITTSHSVRQRFLIAVAIGTSIGWYDGFFGPGTGSFF
                     IFLLVRWLGQDFLHASASAKVLNATTNLAALLMLAATGHVWWQVGLMMAAANVAGSLL
                     GTALALKHGAQLVRRVFMLVVAVLIIRTAWQAYF"
     gene            complement(2959..3798)
                     /locus_tag="VNZ84_03885"
     CDS             complement(2959..3798)
                     /locus_tag="VNZ84_03885"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HWU66127.1"
                     /translation="MARYAIGDIQGCYHSLMNLLEQLDFQPGQDTLWLVGDLINRGTG
                     SLAVLRWVFAHQHCVRVVLGNHDLHTIAVAHHIKPPRRFDTLSELLSATDGHELLHWL
                     RQQPLMVLEADFAMLHAGLYPQWTLAQARALAAEVEAVLQSPQYLGFLQQMYGNQPAA
                     WQDNLTGIDRLRAITNAFTRMRLLDAEGTMEFAFKGELADVPAGCMPWFLSPERQSRH
                     DPQTILFGHWSALGLYQGHGVMGLDTGCLWGRQLTAYALETGHISQVPLDPRDRPADL
                     AGD"
     gene            complement(3877..4380)
                     /locus_tag="VNZ84_03890"
     CDS             complement(3877..4380)
                     /locus_tag="VNZ84_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWU66128.1"
                     /translation="MSGNNQSSSIKELIIAIPGAIIGFTLLVSILAYLFGVVGGEKAE
                     PSKEATDAAEQKVVEMIKPVATVEVAEAAGAHVEKSGEEIVKGVCIACHGVGALGAPK
                     IGNAGDWGPRIAQGYETLVKHAIEGIRSMPARGGNAELSDHEVADAVAYMANQAGAKF
                     DAASLKK"
     gene            complement(4526..6013)
                     /locus_tag="VNZ84_03895"
     CDS             complement(4526..6013)
                     /locus_tag="VNZ84_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HWU66129.1"
                     /translation="MKKMTLMLALALGSISSAQAAITYEISKEWTFTHTGGGYLSEIP
                     AFDSVTNTLWVAGVKGVDVLDATTGSLLQRIDTSAYGNINSVSIKNGLAAFAIENTAR
                     TQAGIVQLYDTSTRSLAAGVNSFTVGALPDMVTFTPDGSKILVANEGTPDVYGSRIGS
                     SVPRVYGAAANDPVGSVSIIDVPSRTVAATATLAGVPTSGTHIRTNTGMDFEPEYIAV
                     SADGSKAYVTLQEANAIGILDLNTNTFTKVVGLGAKDFSLPGNTIDPLNNGSVSFISA
                     NVKGLYMPDGIATYDVAGKTYLVTANEGDFREDDGDRSAASSMGATGELANIRVSNTD
                     SSPGDLYAAGARSFSIRDADGNLVYDSGDILDKEAFARGIYDDGRSRDKGVEPEGVDL
                     FALGGRTIAAVGLERTLKGAIALFDITDPTKASFIDMIVSDGDLAPEGLVAFEKNGAI
                     FLAFANEGSNTTSLFRISAVPEPGSAAMLGLGLVLMAAMRRRRQQ"
     gene            complement(6150..7289)
                     /locus_tag="VNZ84_03900"
     CDS             complement(6150..7289)
                     /locus_tag="VNZ84_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HWU66130.1"
                     /translation="MKAMILAAGKGTRVRPLTYELPKPMIPVLGKPVMAYLIEHLAMH
                     GVADIMINVSYLHKKIQDYFEDGHRFGVNIGYSFEGDIVDGQVVPHAVGSAGGMRKIQ
                     DFSGFFDETTIVLCGDAIIDLDITRAVKEHKEKGALASLVTMQVPMDKVSGYGIVVTN
                     KEGRIISFQEKPRQQEARSNWASTGIYIFEPEVLDLIPSGSEFDIGSDLFPLLVEKGL
                     PFYAIQHPFNWIDIGVVTDYWLVMQQMMQGFFPSMPIPGKQIKPGVWVGLNVHIDWEH
                     THIEGPVYIGSGSRIDKNARIIGPTWVSSGCHIQRDSTVIRSILFDYTRIAQGYEIEE
                     RIVCGEYCVDRKGRMVHIEDDNCDIIWTDAREKVVYPQNYAYARL"
     gene            7446..7522
                     /locus_tag="VNZ84_03905"
     tRNA            7446..7522
                     /locus_tag="VNZ84_03905"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7480..7482,aa:Arg,seq:ccg)
     gene            7495..7929
                     /locus_tag="VNZ84_03910"
     CDS             7495..7929
                     /locus_tag="VNZ84_03910"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HWU66131.1"
                     /translation="MGSTPAGRTSQKSKAAEESKKKQQAKPTKPKNTTPVDPVVLIIG
                     KLQKLYPKAFPKNPTPKVPLKIGILEDLQARSEEISISKEDLQLAIKTWCKSSRYWQA
                     CKEGADRLDLDGNPVGVVETTGAAHAKGMVRKQAKAKQTPTE"
     gene            complement(8122..8217)
                     /locus_tag="VNZ84_03915"
     CDS             complement(8122..8217)
                     /locus_tag="VNZ84_03915"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66132.1"
                     /translation="MLANGKAGSVEVKQSSGRKILDEEAQDVVKA"
     gene            8449..8748
                     /locus_tag="VNZ84_03920"
     CDS             8449..8748
                     /locus_tag="VNZ84_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU66133.1"
                     /translation="MSDVVAKRLKEARLAKGLSQKKLGIAAGIDEFTASPRMNQYERG
                     VHIPDYSTLERLAQVLEVPVPYFYAADDELADWIKCFPRLGKAEREKIIAASSND"
     gene            complement(8883..9785)
                     /gene="cynR"
                     /locus_tag="VNZ84_03925"
     CDS             complement(8883..9785)
                     /gene="cynR"
                     /locus_tag="VNZ84_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HWU66134.1"
                     /translation="MILRHIHYFLAVAEHGNFTRAADVLHVSQPTLSQQIRQLEESLG
                     TVLLDRSGRRVRLTDAGEVWARHAKLALRELDSGARAVHDVAELSRGVIRLAATPTIT
                     SYLAGAVISEFHNLYPGITIDLHEIPQAQIELMLADDKLDLGIAFDHAQSAEIEYQSL
                     FSETLSLVIEKKHVLMESVNASSVKVLTTEPLVLLNTSFATRQHIDEYCRLLNIRANV
                     VIATNSISAILQIVRKGHISTILPSQIDLEGTDLYSLQLDPPLPARKVVILRRKDAYQ
                     TAAVRAFTQVLIDYADCIMARTGG"
     gene            10296..11729
                     /gene="ccoN"
                     /locus_tag="VNZ84_03930"
     CDS             10296..11729
                     /gene="ccoN"
                     /locus_tag="VNZ84_03930"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HWU66135.1"
                     /translation="MQSFNKSEFFNDTVVRQFSIMAIVWALIGTMVGVLIASILVWPE
                     IGAGIPELSFGRLRPVHTNLVVFAFGGCSLFATSYYVVQRTCQTSLFLPKLASFTFWG
                     WQAALVAAAISLPLGYTQGKEYAEMEWPIDVLMAVVWISYAVVFFGTIGIRKVRHIYV
                     ANWFYGAFILAVAMLHIMESAAIPAGWFKSYSAYAGVQDAMVQWWYGHNMIGFFLTAG
                     FLGMMYYFVPKQAERPVYSYSLSIVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVL
                     SLILFAPSWGGMINGIMTLSGAWHKLRTDPILRFLIVSLSFYGMSTFEGPMMAIKTVN
                     ALSHYTDWTVGHVHSGALGWVGFISIGSLYFLIPRLFGQKTMYSVKAIEVHFWMATIG
                     VVLYIAALWISGVMEGLMWRAMNADGTLAYTFVESVKAKFPYYFTRALGGFLYMSGFA
                     LMLWNTVKTARIGKPAAVAVPNALAHA"
     gene            11739..>12225
                     /gene="ccoO"
                     /locus_tag="VNZ84_03935"
     CDS             11739..>12225
                     /gene="ccoO"
                     /locus_tag="VNZ84_03935"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HWU66136.1"
                     /translation="MASHHQTNCNLHEKIETNNFLLIVLTFITISFGGLVEIVPLFFQ
                     KSLTEPIRGLQPYTALQLAGRDIYLREGCYNCHSQMIRPFHAETLRYGHYSVAGEYVY
                     DHPFQWGSKRTGPDLHRVGGKYSDDWHRVHLNQPRDVVPESIMPAYPWLNDNLVDDTT
                     IG"
BASE COUNT         3162 a         3225 c         2826 g         3012 t
ORIGIN      
        1 caatcctggg cgcctgcgcc agttccaact ccggtagcgt gtactcacgc gacgaagccc
       61 gccgggcaca gaccgtgaaa accggggtgg ttgaaagtgt acgcccggtc aaactggaag
      121 gcaccaaatc ccctgttggt acagtagcag gcggtgctat tggcgggatt gccggcagct
      181 cgattggtag cggcaaaggc agtgcaattg ctgcagtgat tggtgcagtg gcaggcggcc
      241 tggctgggtc agcagcggaa gaagtcgcta cccgcaaaaa tgcgcttgaa atcaccgtca
      301 aactggatgg cggcggcttg gtagccattg tgcaagaagc agacgagcaa tttaacgcag
      361 gcgagcgtgt acgcattgtt gaaagtggtg gcacctcccg cgtttcacac taaaccaatc
      421 acctcacaaa agagcggcga taagccgctt tttttattgt ccactcaccc aaacatcgtc
      481 acacaactgt cacacgaacg tcaccaatca ttcatgtgac acttctaata tcacgctaaa
      541 cctcaatcaa ggagtcgcgt gatgttagaa cgcaatctgc aaaatctcgg cacatatcag
      601 ccagacattc aaccatcagc taatagcagc cgcattccga gcaccacgct cagtgtgtcg
      661 ctcgcctcta gtgcagaaga aattatggaa gcgcagcgct tgcgctacca ggtattcgca
      721 gaagagatgg gcgcacagat caaaagccag aacggactgg atgtcgatgg ctttgatgct
      781 ttttgtgacc atttagttgt acgcgacgac gcaacacaac aaattgttgg tacataccgc
      841 attttaaatc caatgcaggc catgctggcc ggggggtact actccgctgg cgagtttgat
      901 ctcagccgcc tggcgccatt ggcagaccgc atggtagagg ttggtcgtgc ctgcgtgcat
      961 gccgactacc gaactggtgg caccatcacc atgttgtggg ccggactggc taaatacatg
     1021 atggcacgtc aatacgaata tatgataggt tgcgccagca tctccatgca cgacggtggt
     1081 cataccgccg cttctctgtt taacaatttg cgcgaagact atttgtcgcc tgccgaatac
     1141 cgcgtgttcc cacacaatcc tttacccatc gaagcgctgg accagtcctt gcccgtgacc
     1201 tgcccgccac tggtcaaagg ttatctgcgt ctgggcgcct acatttgcgg cgaacccgcc
     1261 tgggatcctg cttttaatac cgcagatatg ctggtattgt tacctttatc cagaatcaat
     1321 cccagatatg ctgcgcattt cttgaaataa cttgccaaaa acaaactttc ttactcgcta
     1381 ttggcgcatc gcccgcattt ttctccacac cctgaccgga ctcacattgg caacgctagt
     1441 gttgcctgtg tgtggccgcc gtggccgcga gcaaatcatt cgctggtggt gtgcaaaact
     1501 cctgcgctgc tttaacttac agttatccgt gagtggccaa tgcccggcat ctcagacaca
     1561 gcacaccctg tttgttgcca accacatttc ctggtcagat atccatgccc tgaatagcct
     1621 gattccggta cgctttgtcg caaaacttga gatcaaggat tggccggtgt ttggatactt
     1681 ggtgaaaaaa tcagggacga tctttatcaa ccgcacacgt caacgcgatg cggcacgcgt
     1741 gcttaaagtc gtcacacaag cgatgaagct cggtgacaat ctatgcgtat ttcccgaagg
     1801 tacgacgacc gatggacagc aggtgctacc gtttaaaagc agcctgatcc aggcgtcaat
     1861 tgatgctgag gcgactgtga tcccgattgc aatccactac cctttacctg acggcagccc
     1921 caatctggcg gctgcttatg caggggaaac gaccatgatt gagtcgatgc tgtctttttt
     1981 aaacatgcag tccccacgcg tacatttaca tttttgtacg ccgatagaag cgcgcctgtt
     2041 ggaccggcaa acgctagcca cccaagcaag aaatgaaatc attgcacact tgccatgggc
     2101 gacgacctct gaggtcgtca gccagccatc actacaaaga caacaagcgt gatggcaatt
     2161 gacgagattg ctgtgaaggg cacctctaaa aataggcctg ccaggccgtc ctgataatca
     2221 gcacggccac caccagcata aatacacgcc tcaccagctg ggcaccatgc ttgagcgcca
     2281 gcgccgtccc cagcaaactg ccggccacat tggcggccgc catcatcaac cccacctgcc
     2341 accatacatg ccccgtggcg gccagcatca gcaaggctgc caggttagtt gtggcattca
     2401 atacttttgc tgaagccgaa gcgtgtaaaa aatcctgtcc cagccaacgc accagcaaga
     2461 aaataaaaaa gctgccagta cctggcccaa aaaagccgtc gtaccagcca atggatgtcc
     2521 caatcgcaac agcgatcaag aaacgttgtc gcacactatg cgaggtggtg atgcgtgaca
     2581 aaccgaattg cggatacttg aaggtgtaga acagtaccag cactaatacc caaggcaata
     2641 atttgcgcag gaaatcggga tccgtccagg tgagcgtcca tgctcccaag cctgatccga
     2701 tcaatgcagc cagtgccgcg ggcgccagcc atctccagtc taactgcaca tgccgaccat
     2761 atcgccacgc cgcaacagca gtaccaaata cactagagga tttatttgtc cccagcagca
     2821 aggccggagc aacattgggg aaagttgtca gtaaagctgg aaccgtgacc aggccaccgc
     2881 caccgaccat ggcatccacg atgccagcca gaaatgccgc cgatatgacc agccataact
     2941 ccatatcgtt tgactgctct aatcgcctgc caaatctgct gggcggtcgc gcggatctaa
     3001 cggcacctgg ctgatatgcc cggtctccag cgcatacgcc gtaagctggc gcccccacag
     3061 gcagcctgta tccaacccca tcacaccatg cccctgatac agtccgaggg cagaccaatg
     3121 cccaaacaaa attgtctgtg gatcatgcct ggattggcgc tcaggcgaaa ggaaccacgg
     3181 catacatcct gccggaacat cagctaattc acctttgaaa gcaaactcca tggtgccttc
     3241 agcatctagc aggcgcatgc gcgtaaaggc attggtaatg gcgcgcaagc gatcaatgcc
     3301 tgtcaggtta tcctgccagg cagccggttg gttaccgtac atttgctgta aaaagccaag
     3361 atactggggc gactgcaaaa cagcttcaac ctccgcagcc agtgccctgg cctgcgctaa
     3421 tgtccactgg ggatacaggc cagcatgcag catggcaaaa tccgcttcga gcaccatcag
     3481 tggttgctgg cgcaaccagt gtagtaactc atggccatcc gtcgcagaca ggagttcaga
     3541 cagggtatca aaacggcgag gcggcttgat atgatgcgcc acagcgatgg tatgcaagtc
     3601 atgattaccc agtacgaccc tcacgcaatg ctgatgtgca aacacccagc gcagcactgc
     3661 cagtgacccc gtaccgcgat tgatcagatc ccccaccagc cacaaagtat cttgcccagg
     3721 ctgaaaatcc agctgctcaa gtaaattcat cagggagtga taacaaccct ggatatcacc
     3781 aattgcgtat ctcgccatgg tttttagagt gatccggaaa caggtttacc aataaaaaag
     3841 ccgcttgatt caagcggctt ttcgcgtttt atctcattac ttcttcaagc tggctgcatc
     3901 aaattttgca cccgcctggt tcgccatata tgccacggca tctgccacct cgtggtcact
     3961 cagctcggcg ttaccaccgc gggccggcat gctacggata ccttcaatgg catgtttgac
     4021 cagtgtttca taaccttgtg caatacgtgg accccagtca ccagcgttgc caatttttgg
     4081 cgcacccaat gcacctacac catgacacgc gatacaaaca ccttttacaa tttcctcgcc
     4141 gctcttctca acatgtgcgc cagcagcttc agccacttct actgtagcaa ccggtttaat
     4201 catttccaca accttttgtt ctgctgcatc agtcgcttct ttggaaggct ctgctttttc
     4261 gccacccact acgccaaaaa gataagccag tatggaaacc agcaaagtga aaccaataat
     4321 ggctcctgga atcgcaatga ttaactcttt aatgcttgaa ctctgattgt tacccgacat
     4381 atccgtatcc cgttgcgtta gaaaaaatat ccacgcgatt ataacgcaga acgtctggac
     4441 gtgaaacgcg cccacggctt ttggcacagg taaaaaacaa acagccaggc atatcaacat
     4501 gcctggctgt gcttacaaaa ataagttact gttggcgacg acggcgcatg gctgccatca
     4561 acaccaagcc caggcccaac atggcagctg aacccggttc aggtacggca ctgatcctga
     4621 ataaggaggt ggtattggag ccctcgttag caaaagccag gaagatagcg ccattctttt
     4681 caaatgccac caatccctcc ggggccagat caccgtcaga cacaatcata tcgataaagc
     4741 tggctttggt tgggtctgtg atatcaaaca aggcaatcgc ccctttgagg gttctctcta
     4801 gccccacagc ggcgatggta cggccaccca aggcaaacaa gtcgacccct tccggctcaa
     4861 cacctttatc gcggctacgg ccatcatcgt agatgcctcg cgcaaaggct tctttatcga
     4921 ggatatcacc actgtcgtaa accaggttgc catcggcatc acggatactg aatgaacggg
     4981 cacccgctgc ataaagatcg cctggcgatg aatcggtatt agagacccgg atattcgcca
     5041 gctcgccagt agctcccatt gagctggctg cggaacgatc gccatcatcc tcacggaaat
     5101 caccttcgtt tgctgtcacc aggtaggtct tgccagccac atcataagtg gcaatcccgt
     5161 caggcatata caagcctttt acattggcac tgataaaact gacactgcca ttattgagcg
     5221 gatcaatcgt attccccggc aagctaaagt ctttcgcacc caggccaacc actttggtaa
     5281 aggtgttggt attcaggtcg aggatgccaa tggcgttggc ctcttgtagc gtcacataag
     5341 ccttgctgcc atctgcgctc actgcgatat attcaggttc aaaatccatg ccggtattgg
     5401 tacggatatg agtaccggaa gttggtacac cagcaagcgt agccgttgct gcaacagtac
     5461 ggctgggaac atcaatgatg gacacactgc cgaccggatc attagctgcg gcgccgtata
     5521 cacgtggcac actgctaccg atacgtgaac catagacatc cggcgtgcct tcattagcga
     5581 ctaaaatctt gctgccatca ggggtaaatg taaccatgtc tggtaatgca ccgactgtaa
     5641 agctgtttac acctgccgcc aagcttctgg ttgaagtgtc gtataactgc acaataccag
     5701 cttgcgtacg cgcagtattt tcaatcgcaa acgcagccaa gccgttttta atactgacgc
     5761 tattgatatt accgtaagcg ctggtatcaa tgcgctgcag caaactgcct gtcgtcgcat
     5821 ccagcacatc cacgcctttc acacctgcca cccaaagggt attggtcaca gaatcaaatg
     5881 cgggaatttc agacaaatag ccgccaccag tgtgcgtaaa tgtccactct ttactgattt
     5941 cgtatgtaat tgcagcctgg gctgagctta tgctgcccag cgccaatgcc aacattaatg
     6001 tcattttttt cattttggta tcctctctca aaagggcttt agaggatact taacgaatat
     6061 gacacatcct tgaaagtggt atgtcccaca gatgtcagcg gactatttta cggtgaataa
     6121 atctaatttc atgtaagcac acacttccac tacagccttg cataggcata attctgcggg
     6181 taaaccactt tttcacgcgc gtcggtccag ataatgtcgc aattgtcatc ttcgatatgc
     6241 accatacggc ctttacggtc cacgcagtac tcgccacaca caatacgctc ttcaatctca
     6301 tagccttgag caatccgcgt gtaatcaaac aagatggaac gaatcaccgt gctgtcacgc
     6361 tggatatggc aaccagagct gacccaagtc gggccaataa tgcgggcgtt tttatcaatg
     6421 cgcgaaccgg aaccgatata caccggccct tcaatgtgcg tgtgttccca gtcaatatgt
     6481 acattcaaac ccacccaaac accaggcttg atttgcttgc caggaatcgg catcgaagga
     6541 aaaaatcctt gcatcatctg ctgcatgacc aaccaatagt ccgtcaccac gccaatatca
     6601 atccagttaa acggatgctg aatggcataa aaaggcaggc ccttctcaac caacaatggg
     6661 aacaaatcgg agccaatatc aaactcactg cccgacggaa tcaggtccag cacttccggc
     6721 tcaaaaatat atatgccggt gctcgcccaa tttgaacgtg cctcttgctg cctgggcttt
     6781 tcctggaacg aaataattcg cccctcttta ttagtcacga caatcccata gccagacact
     6841 ttatccatag gcacctgcat cgtcaccaga ctcgccagcg cacctttctc cttatgctct
     6901 ttcaccgcgc gcgtgatgtc caggtcaata atggcatccc cgcacaagac aatcgtcgtc
     6961 tcatcaaaaa aaccgctaaa gtcctggatt ttgcgcatgc cgccggcaga acctacggca
     7021 tgaggcacca cctgcccatc cacaatatcg ccttcaaacg aatagccaat attcacccca
     7081 aaacggtggc catcttcaaa gtaatcctga atttttttgt gcaggtaact cacattaatc
     7141 atgatatcgg ccacaccatg catcgccaga tgctcaatca ggtacgccat cacaggctta
     7201 cccagtacag ggatcatcgg ctttggtaac tcatacgtca gcggcctgac gcgtgtccct
     7261 ttacctgcag ctaaaatcat ggctttcatc atgcaacacc cttaaataac ttaacgttca
     7321 ccggccaagc atagccagcc atgatgacaa gattatttca gcagcggagt accagaaagt
     7381 gcagcataaa aattggctgc atccgcgcca agcctgggtc acttatttgc tataatggcg
     7441 ggattgcgcc cgtagctcag ctggatagag tattggtttc cgaagccaag ggtcgtgggt
     7501 tcgactcccg ccgggcgcac cagtcagaaa agcaaagccg ctgaagaatc gaagaaaaaa
     7561 cagcaagcga agccaaccaa gcccaaaaac acgactcctg tagatcctgt ggttttaatc
     7621 ataggcaaac tccagaaact ttaccccaaa gcattcccta agaacccgac acctaaagta
     7681 ccattgaaga ttgggattct agaagacctt caagcaagat ccgaagagat tagcatttca
     7741 aaggaagact tacagctggc cattaaaact tggtgcaaaa gctcacgtta ctggcaagca
     7801 tgcaaagaag gcgctgatcg tttggattta gatggtaatc ccgttggcgt ggttgagacg
     7861 accggagcag cacatgcaaa agggatggtg cgaaaacaag ccaaggccaa acaaactccg
     7921 acagagtaat ttttgtttga gaatttatta accctaataa ctaaatcgct cacaactacc
     7981 tgtgtgagcc ttatttcatg ggcactatgg cctttaccaa gaaaacagga ccacacaaaa
     8041 ttatcctcac actcgaaatt caatcggaac agtggcccat ccatcaagag gcgtatcacc
     8101 acgcttagct ggtgcaaaca ttcatgcctt cacaacatct tgcgcctctt catcaagaat
     8161 ttttcgtcca cttgactgtt ttacctcaac tgaaccggcc ttaccattag caagcacacg
     8221 aacacgtaaa attaccctgc cccccaacct tgacgctgtg caaataaagg atatacaggt
     8281 gcaggattat ttaaatatcc tgcatagcca gtagccttag tctcagctct tccttaggta
     8341 ctggcggtgg ctgcacaact ggaccggcag ccacagactc agtcgatgtt gttggtgctg
     8401 cagctacagg cccatctctg agtacctaaa acgtaccgat gattcttaat gagcgatgtt
     8461 gttgcaaaga gattgaaaga agcgagattg gctaaaggac tttctcaaaa gaagctcggt
     8521 attgcagcag gtattgatga gttcacagcc agcccaagaa tgaatcagta tgagcgtggc
     8581 gttcacattc cagactattc aacacttgag cgtctagcac aggtattaga agtgcctgtg
     8641 ccttatttct atgcggctga tgatgaattg gctgactgga taaagtgctt tccaagacta
     8701 ggcaaagctg agcgagaaaa aatcattgct gcatctagta acgactagaa cgcggcgagc
     8761 ataatgtcgc ggtgatgagt ctgctgaaga ttgcagatgc tttagatttg aagcttagtg
     8821 atgtattggt tagttgcgaa ctttagatgc aatctgaata gcgagtgcaa ctagataatg
     8881 gcttagccgc cagttcttgc catgatgcaa tccgcatagt caatcaacac ctgagtaaat
     8941 gctctcactg cagctgtttg atatgcatct ttgcgcctta aaatgactac ctttctcgca
     9001 ggcaagggtg gatcaagctg aagagaatat aaatcggtac cctctaaatc aatctgtgag
     9061 ggtagtattg tcgatatatg cccttttcgt acaatttgta aaattgcgct aatagaattc
     9121 gttgcgataa ctacatttgc tctgatgttc aatagtcgac aatattcgtc aatatgctga
     9181 cgcgtcgcaa atgatgtatt gagtaatacc aggggttctg tcgtgagcac tttaactgat
     9241 gaagcattaa cactttccat cagaacatgc ttcttttcaa tcactaaact tagcgtttca
     9301 gagaatagtg attgatattc gatctcagcc gattgtgcat gatcgaatgc aattcctaaa
     9361 tcgagtttat catcagccag catcaactct atttgcgcct gcggtatttc gtgtaagtcg
     9421 atagttatgc cgggatagag gttatgaaat tcacttatta cagcgcctgc taaatacgaa
     9481 gtgattgttg gcgttgctgc caatcttatg accccacgag atagctccgc gacatcgtga
     9541 actgcccgag cacctgaatc aagctcacgc agagcaagtt ttgcatgccg cgcccacacc
     9601 tcacctgcat ctgttaagcg cactctacga ccagagcgat caagtagtac agtgcctagg
     9661 ctttcttcaa gctgcctgat ttgttgtgac aaggtcggtt gtgaaacatg cagaacatca
     9721 gccgctcttg tgaaattgcc gtgctccgcg acagccaaaa aataatggat atgacgaagt
     9781 atcattgcaa ttcctagtat tggtttttcc tatggaatct attgtaaatc agtcttggac
     9841 gctataggta gattcctcca aaattcagcc agcttaagga agggtttaaa tcatgaaaga
     9901 agtaatcgaa ggctgcctga aactccaacg ggatatatca cccccctcgt agagtgcatt
     9961 tcgccaacta gcgtcaagtc aaacaccgca tactttgttg atttcgtgaa cggaagtcgt
    10021 ctggttctga tgtagtcagc gattatcaag ttttaaaacc ggcttttcta catcacacaa
    10081 ggtatagatt ttgcgactca tctaactctt tctaaagttt cattgaattt gccaatggac
    10141 tcagctcact ctctatattt accccatctg atatgcaaat tttgtaaatt tatgaccctg
    10201 ttttcttcac tcacttaacg tcattatgct cgctgctcta tgtgctacaa cttgaaggaa
    10261 gcatggggca atcaactctt agaggaaaaa tggacatgca gtctttcaat aagtctgaat
    10321 tttttaacga tacggtcgta cgccagttct ccatcatggc aattgtgtgg gcgcttattg
    10381 ggaccatggt cggcgtgtta atcgcgtcta ttttggtatg gcccgaaatt ggagcaggta
    10441 ttcctgagct gtcattcggc aggttacgcc cagtacacac aaacttagta gttttcgcat
    10501 ttggtggctg ctctttgttt gcgacctcat actatgtggt tcaacgcact tgccaaacaa
    10561 gtttgttttt accgaaattg gctagcttta ctttctgggg ttggcaggct gcgcttgttg
    10621 cggctgccat ttctctgcca ttgggctata cccagggtaa agagtatgca gaaatggaat
    10681 ggccaatcga tgttttgatg gcagttgtat ggatatcata tgcagtagtg ttttttggca
    10741 ctataggcat ccgaaaggtc aggcatattt acgtggctaa ctggttctat ggtgcgttca
    10801 ttctggcggt ggcaatgtta cacattatgg agagtgctgc gatcccggct ggctggttca
    10861 agtcttactc tgcttacgca ggtgtgcaag atgcgatggt gcagtggtgg tatggtcaca
    10921 acatgattgg ctttttcctg actgctggtt ttctgggcat gatgtattac ttcgtgccta
    10981 agcaagctga gcgtcctgtt tactcttaca gcttgtctat cgtgcacttc tgggcactga
    11041 tcttcactta tatgtgggcg ggtcctcatc acctgcatta caccgctttg cctgactgga
    11101 ctcagtcttt gggtatggtg ttgtccttaa tcctgtttgc accaagctgg ggcggtatga
    11161 tcaacggtat catgaccttg tccggcgcat ggcacaaact gcgcacagat cctatcttgc
    11221 gcttcctgat cgtttccttg tctttctacg gtatgagtac ctttgaaggc ccgatgatgg
    11281 cgatcaagac tgtgaacgct ttgtctcact acactgactg gaccgtgggt cacgtgcact
    11341 ctggtgctct gggctgggtt ggtttcatct ctatcggttc cctgtacttc ctgattccac
    11401 gtttgtttgg tcaaaaaacc atgtacagcg taaaagcgat tgaagtgcac ttctggatgg
    11461 caaccattgg cgtggtgttg tatatcgctg cgttgtggat ctctggcgtg atggaaggcc
    11521 tgatgtggcg tgcgatgaac gctgacggta cattggctta taccttcgtt gagagtgtaa
    11581 aagctaaatt cccgtactac ttcacacgtg cattgggtgg tttcctgtac atgagtgggt
    11641 ttgccttgat gctatggaac acagtaaaaa ctgcccgcat tggcaaaccg gcggcagttg
    11701 cggttcccaa tgcattggca catgcctaag gaataaaaat ggcatctcac catcaaacta
    11761 actgtaacct ccacgagaag attgaaacca ataacttcct gttgatcgtg ctgactttca
    11821 tcacgatctc ctttggtggc ctggttgaaa tcgtgccttt gtttttccaa aaatccctga
    11881 ctgaacctat ccgtgggttg cagccataca ctgcattgca attggcaggt cgtgacattt
    11941 acctgcgtga aggttgctac aactgccact cacagatgat ccgtccgttc catgctgaaa
    12001 cactgcgtta tggtcactac tctgtagcgg gtgaatacgt gtatgaccac ccgttccagt
    12061 ggggcagcaa gcgtaccggt cctgacctgc accgtgtagg cggtaaatac agtgacgact
    12121 ggcatcgtgt tcacttgaac cagccacgtg acgttgtgcc ggaatccatc atgccagcct
    12181 acccatggtt gaatgacaac ttggtagacg acacgaccat tggtg
//
LOCUS       DATMSK010000114         5176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_196528,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000114 DATMSK010000000
VERSION     DATMSK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196528"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VNZ84_03940"
     CDS             <1..332
                     /locus_tag="VNZ84_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HWU66137.1"
                     /translation="MASVALWTQGVIFMESIFCRVSAQFTQNIFAKLDPRGRGYLDQN
                     VLERALGDVTEEEVRQLISTLDKDGDERVYPYELNLAILDCLNEPDPLPMSRPFTCLL
                     REYGLVE"
     gene            complement(341..730)
                     /locus_tag="VNZ84_03945"
     CDS             complement(341..730)
                     /locus_tag="VNZ84_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HWU66138.1"
                     /translation="MKNLCPCHSGKSYTDCCQPLHLGQVAADAERLMRSRYSAYTLKL
                     PDYILQTWHPDTRPKSLGMEEMHGIKWLKLQVISHQQQNETLAEVEFIATYQSGKQKK
                     STLTEHSRFTKIQGQWFYVGEIKADQR"
     gene            complement(794..1258)
                     /locus_tag="VNZ84_03950"
     CDS             complement(794..1258)
                     /locus_tag="VNZ84_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66139.1"
                     /translation="MSAFMPTGILKAPSLKQPFAWLIANGYLLVDDRSWGTQYRGPIL
                     IHASKGLYEEYYQYIKSHTDIPIPERDQLDYGGVVGIARLVLCCKPGELPADLSREQR
                     VHFGGVHAEYYGFLFAQARPLPWMPCAGKLGIFEIDIDALRAAPPPAQSALF"
     gene            complement(1326..2900)
                     /locus_tag="VNZ84_03955"
     CDS             complement(1326..2900)
                     /locus_tag="VNZ84_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HWU66140.1"
                     /translation="MSSLQQEVERRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEV
                     RGRKAARHATSDWMELEKQRGISVTSSVMQFPYREHMVNLLDTPGHDDFSEDTYRTLT
                     AVDSAVMVIDSVNGVEAQTIKLLNVCRMRDTPIITFINKLDRESRAPIELLDEIETTL
                     GMECAPMTWPIGMGKTFRGVYNLVTDSILFFDPRAEKGTSETIQGIDNPRLDELIGYQ
                     AEELRVDIELVRGASNEFNKERYLSGKQTPVFFGSAVNNFGVQSLLDAVVDLSPPPQS
                     RNTATRVVAANEEKFAGFVFKIQANMDPKHRDRIAFLRVCSGRFERGMKIKQVASGKM
                     LNVNNAITFMAQDRTIMDEAYSGDIIGIPNHGTIKLGDTFTENEALKFIGIPSFAPEF
                     FRRARIKNPMKMKQLQIGLKQLSEEGAAQLFRPLLSNDLILGAVGMLQFDVVAHRLEH
                     EYGVDMVFEPYDCYTARWLKGSDADLKAIADKYGFNVGLDSADDYVYLAPNRVNLQMA
                     QERYPDIQFMETREIA"
     gene            complement(3035..5011)
                     /gene="acs"
                     /locus_tag="VNZ84_03960"
     CDS             complement(3035..5011)
                     /gene="acs"
                     /locus_tag="VNZ84_03960"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWU66141.1"
                     /translation="MSIESVLHEHRVFAPAKAFSSQAAVGSMEEYQRLHAEALQDYEG
                     FWAKLARELLIWHKPFTKTLNEDNAPFYKWFEDGELNVSANCLDKHLQTIPNKTAIIF
                     EADNGDVKNVTYKELYHQVCQFANGLKSLNLVKGDRVIIYLPMGIEAIVAMQACARLG
                     IIHSVVFGGFSAKSLNERIIDAGAVAVITSDGQYRGGKTLPLKQAVDEGIAMGGCDSI
                     RHMVVFKKTGQEVSWDNKCIWWHELIADKADTCEPVMVEAEHPLFLLYTSGSTGKPKG
                     VQHSSAGYLLHAMNTMRWTFDIKPEDIYWCTADVGWITGHSYVTYGPLAMGATQIVFE
                     GIPTYPNAGRFWEMIQRHKVSVFYTAPTAIRSLIKAGETDFTTHPNHFDLSSLRLLGT
                     VGEPINPEAWMWYYEQVGGNRCPIVDTFWQTETGGHVITPLPGATPLVPGSCTLPFPG
                     IDIDVVDETGKDIPWGSGGLLVIKKPWPSMIRTIYNDPERYKASYFPAELGGNLYLAG
                     DGAVRDATTGNFTIMGRIDDVLNVSGHRLGTMEIESALVANHLVAESAVVGKPHPIKG
                     ESVVAYVVLKGKRPEGEEAKKIVAELRDWVGKEIGPIAKPDEIRFGDNLPKTRSGKIM
                     RRLLRSLAKGEEITSDISTLDNPAILEQLKQAVN"
BASE COUNT         1214 a         1279 c         1353 g         1330 t
ORIGIN      
        1 aaatggcttc tgtcgcatta tggactcaag gagttatatt tatggaaagt attttttgta
       61 gggttagtgc acaatttacc cagaatattt ttgctaaact ggatccacgc ggccgcggat
      121 accttgatca gaatgtactt gagcgtgcct tgggtgatgt gaccgaggag gaagtcaggc
      181 aattgatatc caccttggac aaggatgggg atgagcgtgt ctatccctat gagctgaact
      241 tggcgattct ggactgcttg aatgagcctg atcccttgcc aatgtctagg ccgtttacct
      301 gccttttgcg tgaatatggc cttgtagagt agacgggtgc ttatctttgg tcggccttta
      361 tttctccgac ataaaaccat tggccttgta ttttagtgaa tcggctatgt tcggtaagcg
      421 tagatttttt ctgctttcca ctttggtagg tcgcaatgaa ctcgacttct gcaagtgttt
      481 cgttttgctg ttgatgggaa ataacctgca actttagcca tttaatgcca tgcatttcct
      541 ccatgcccag gctttttggc cgcgtgtctg gatgccaggt ttgcaggata tagtcgggta
      601 atttcaaagt ataagcacta tagcgtgagc gcatgagccg ctcggcatcc gctgccactt
      661 gtcccagatg taaaggctgg caacagtctg tatacgattt gccgctatgg cagggacaaa
      721 ggttcttcac tatgagtcct taaacaaaag tacttgaatg gctgccatgt catggctaac
      781 tagcggaggc acttcaaaat aaagcggact gcgctggtgg aggcgctgcc cgtaatgcat
      841 caatatcaat ctcgaaaatg cctaatttgc cagcacacgg catccacggt aatgggcggg
      901 cttgcgcaaa taagaaaccg tagtactctg catgcacgcc gccaaaatgc acccgttgct
      961 ctcggctcaa atcagcaggc agctcaccag gtttgcagca caagaccagc ctggcaattc
     1021 ccaccacacc accataatcc agctgatccc gctctgggat agggatgtca gtatgcgatt
     1081 tgatgtactg gtaatattcc tcatacaagc ctttgctggc atgaatgagg atagggccgc
     1141 gatattgtgt cccccagctg cgatcatcta ccagtaaata gccgttggcg attaaccatg
     1201 caaacggttg tttgagggag ggcgccttga gaatgcccgt gggcataaat gctgacatag
     1261 ttgaactgct agaagggctg tggtagaggg gctggagatt cacgtccagg ccctcaatga
     1321 tgcctttagg cgatctctcg cgtttccata aactgaatgt ctggatagcg ctcttgcgcc
     1381 atttgcaggt tgacgcggtt aggcgccagg taaacatagt cgtcggcact atcgaggcca
     1441 acgttaaagc catacttgtc ggcaatggct ttcaaatctg catcagagcc ttttagccag
     1501 cgcgcggtgt agcagtcgta gggttcaaac accatatcga caccatattc atgttcgagg
     1561 cggtgtgcaa ccacatcaaa ctgcagcata cccacggcgc cgagaatcag gtcattcgac
     1621 aatagggggc ggaataactg agccgcacct tcttcggata attgcttgag gccgatttgc
     1681 aattgcttca ttttcatcgg gttcttgatg cgtgcgcggc ggaaaaattc tggcgcaaat
     1741 gaggggatgc cgataaattt aagggcttcg ttttcagtaa acgtgtcgcc cagtttgatg
     1801 gtgccgtggt tagggatgcc gatgatatcg ccggagtagg cctcgtccat gatggtacgg
     1861 tcttgcgcca taaacgtgat ggcgttgttg acgttgagca tcttgcccga agcgacttgc
     1921 ttgatcttca tgccgcgctc aaagcggcca gagcacacac gcaaaaacgc aatccggtct
     1981 cggtgtttgg gatccatatt ggcctggatc ttgaatacaa agccagcgaa tttttcttcg
     2041 ttggccgcga cgactcgcgt ggcggtattg cgggattgcg gcggcggaga taagtcgacg
     2101 acggcatcca gcagggattg cacaccaaag ttattcacgg ctgagccgaa aaataccgga
     2161 gtttgcttgc cgctcaaata acgctctttg ttaaattcgt tagaggcgcc acgcaccagt
     2221 tcaatatcaa cacgcagttc ttctgcctgg taaccgatca gttcatccag gcgtgggtta
     2281 tctatgccct gaatggtttc tgaagtccct ttttcggcgc gcgggtcaaa aaacaggatg
     2341 ctatctgtca ccaggttgta tacgccacgg aaggttttac ccataccaat tggccatgtc
     2401 ataggcgcgc attccatgcc taaagtggtt tcaatttcgt ccagtaattc tatcggtgcg
     2461 cggctttcgc ggtccagctt gttgatgaag gtgataatcg gtgtgtcgcg catgcggcag
     2521 acgttgagca gcttaatggt ttgtgcctca acgccgttaa ccgagtcaat taccatcaca
     2581 gccgagtcga ccgcggtaag tgtccggtaa gtatcttccg agaagtcatc gtgacctggg
     2641 gtatccagca agttcaccat atgttcgcgg tatgggaact gcatgacgga ggaggtcacg
     2701 gaaatgccgc gttgcttttc cagctccatc cagtcagagg tcgcatggcg agcggctttg
     2761 cggccacgca cttcaccggc cacctgaatc gcgccaccaa accataataa cttttccgtc
     2821 aacgtggttt tacccgcatc cgggtgcgaa ataatggcaa aggtacggcg gcgttcaact
     2881 tcttgctgca aactggacat gaaaaaatag cgctttggaa aaaagcgcta ttttaaccta
     2941 atcaatcact tggccggatt attttcgcat caggaagatg gcgaccgcgg cgaaaataac
     3001 cagaataatt aaaccttcca tcgtaggcag agaattaatt aacagcttgt ttcaattgct
     3061 caagaatcgc cgggttatcc agggtggaga tatcggaagt gatttcctcg cccttggcca
     3121 ggctgcgcaa caagcgccgc atgattttgc cagagcgagt ttttggcagg ttgtcaccaa
     3181 aacggatttc gtccggtttg gcaatcgggc caatttcctt gccaacccag tcgcgtagct
     3241 cagccacaat tttcttggcc tcttcaccct ctgggcgctt gcccttgagc acgacgtaag
     3301 ccacgactga ctccccttta atcgggtgcg gtttgccgac cacggcagat tctgccacca
     3361 agtggttggc gaccagcgct gattcaattt ccatggtgcc taaacggtgt ccagagacgt
     3421 ttaacacgtc atcaatccgg cccatgatgg tgaagttgcc ggtagtggca tcacgaactg
     3481 cgccgtcacc tgccagatag aggttgccgc ccagttcagc cgggaaatag ctggctttat
     3541 aacgctcagg atcgttgtaa atcgtgcgta tcatggaggg ccatggtttt ttaattacca
     3601 ataggccgcc gctaccccag ggaatgtctt taccggtttc gtcgaccaca tcaatgtcta
     3661 tgccggggaa gggcagggtg caggagcctg gtaccagtgg ggtcgcgcca ggcagtggcg
     3721 tgatcacgtg gccgccggtt tcagtttgcc agaaggtgtc tacaatcggg cagcggttgc
     3781 cgccgacttg ctcgtagtac cacatccagg cttccgggtt gatcggctca cccacggtgc
     3841 ccagcaagcg caggctggat aaatcaaaat ggttagggtg tgtggtgaaa tcggtttcgc
     3901 ctgctttaat cagtgagcgg attgctgttg gagcggtgta aaacacgctg accttgtggc
     3961 gttggatcat ttcccagaaa cggcctgcgt tagggtaggt tgggatgcct tcaaatacga
     4021 tttgtgttgc gcccatggcg agcggtccat aagtcacata gctgtgacca gtgatccagc
     4081 ccacgtcggc cgtacaccaa tagatatctt ctggcttgat atcaaaagtc cagcgcatgg
     4141 tgttcatggc atgcagcaga tagcccgcag agctatgttg tacgccttta ggtttgccgg
     4201 tagagcctga ggtgtatagc aggaaaagtg gatgttcagc ttccaccatg acgggctcgc
     4261 atgtgtcagc cttgtctgcg atcagttcat gccaccagat acatttattg tcccagctga
     4321 cttcctgccc tgtttttttg aataccacca tatggcggat gctgtcacag ccgcccatcg
     4381 caatgccttc gtccacggcc tgtttcagtg gcaaggtttt gccgccacgg tattggccgt
     4441 cagaggtgat gacagcgact gcacctgcgt caataatgcg ctcgttcagg ctcttggccg
     4501 aaaagccgcc aaacactaca gagtggataa tgcccaagcg tgcacaggct tgcatggcga
     4561 caatcgcttc aatgcccatc ggcaggtaaa tgatgacgcg gtcgcctttg accaggttca
     4621 gggattttag gccgttggca aactggcaca cctggtgata cagctcttta taggtgacat
     4681 ttttaacgtc cccattgtca gcttcaaaaa taattgcagt tttattggga atggtttgca
     4741 ggtgtttgtc caggcagttg gcagagacat tgagttcgcc atcttcaaac catttataaa
     4801 aaggcgcatt gtcttcattc agcgttttgg tgaatggttt gtgccaaatc aataactcac
     4861 gtgccagttt ggcccaaaat ccttcgtaat cctgcaatgc ttctgcatgc aagcgctgat
     4921 actcctccat gctgcctacc gctgcctggc ttgaaaaagc cttggccggt gcaaatacgc
     4981 ggtgttcatg aagtacagat tcgatagaca tagcgtcccc caaaaatcaa tgtcaaaaaa
     5041 tgaaagtgtg agcgaacctc aggtattatt gttttggttc tgattgacaa actctccgag
     5101 cacacaactc attacaagag caaaaactca tgcaacaact tgtccaaatt ctaacagaaa
     5161 agcaggtcgc gtctac
//
LOCUS       DATMSK010000115        12629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_8938, whole
            genome shotgun sequence.
ACCESSION   DATMSK010000115 DATMSK010000000
VERSION     DATMSK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8938"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VNZ84_03965"
     CDS             <1..464
                     /locus_tag="VNZ84_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWU66142.1"
                     /translation="EYEAAVAQVEKIKLDLERTVVRSPVDGWVSNLLVRPGDFAQVGA
                     AKLAVIDQHSFWVYGYFEEHKLSMINVGDQAEIQLLGSHTTLKGHVESIAHGITDRDN
                     PTDIRLLANVNPTFNWVRLAQRIPVRIHLDQIPAAMPLVAGMTCSVVIRHG"
     gene            535..1626
                     /locus_tag="VNZ84_03970"
     CDS             535..1626
                     /locus_tag="VNZ84_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HWU66143.1"
                     /translation="MLTEKPPQSLLSLLISIIAYLVLAQADKIMHIGQPESLVISILF
                     VLLIINLAFQLSFQVEYVAERLKEPYGTMILTISAVIIEVVIIVMMLSHTSSTTLARD
                     AIYSAVMLDINGILGLCAIVGGIKYGEVEYNVNSGNTYIVMIMTALGISMAMPAFLPP
                     EHWRLYSMFTIVTMSVFYALFLKMQTGRHRNYFSYRDQHQANLLAGMPKAISANVKFS
                     ILYILAGIVTIGFLSEEMSKHMDAGLTGSGVPPIVAAIVVAIIAASPEVLTALKAAVN
                     DRMQTVVNIALGATLSTVILTVPVIEAIALIEDRPLIVGLSPTQMVMTFLTLIVATIN
                     LHDGETNAIEGMTHFVLFMAFAMLAALGM"
     gene            1657..2616
                     /locus_tag="VNZ84_03975"
     CDS             1657..2616
                     /locus_tag="VNZ84_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HWU66144.1"
                     /translation="MHTVLELIQQYGLLIVFASVFLEQMGLPLPAYPTLLLAGVLIGN
                     GQYSWAAMLLVAIVAALAADSIWYRAGRKYGKRVMGKLCKISLSPDTCVRQTEALYLK
                     FGPPALLVCKFIPGFASISSALAGSSGTRYWLFALMDGLGALLWAGSALWLGYLFSSA
                     IDELMLTLVEMGKWGTLLVLVLLVAFIASKWWDRQRFIKSLRMARISVDELNTMLNNG
                     AAPVILDTRAKHLIDDGWIPGAQFVTLEDVDQLVLEIDEDAPVVLYCSCPNEVTAAKV
                     AKKLIGRGYRNIRPLTGGIDAWRDAGFEVTMPEKEAMTKEAHV"
     gene            2609..5782
                     /locus_tag="VNZ84_03980"
     CDS             2609..5782
                     /locus_tag="VNZ84_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU66145.1"
                     /translation="MFNVVGIALKRPYTFVVMALLILIVGTLSAIRSPVDIFPEIRIP
                     IVAVAWQYTGLPPDDMAGRITTLYQRSLTTTVNDIEHIEANSYIGFAIVKIFFHPGVN
                     IATANAQVTAISQTVTRQMPTGTMPPLILNYNASTVPILQIALSGKGLTEQNLADLGL
                     NAVRLRLVTVPGAGVPFPYGGKSRQIQIDLNPAALQARGLSAQDISNALAAHNMIVPV
                     GTQKIGSIEYTLNLNNAATAIDDLAAIPIKVVNGATVYMRDIAQVHDGSAPQTNVVHV
                     DGNRSVLMTILKNGAISTLAIVEGIRSKLEEIKPSLPDALVATPINDQSLFVRAAIEG
                     VVFEGVLAAILTSLMILLFLGSWRSSLIIATSIPLSVLGAIMGLSLIGETLNIMTLGG
                     LALAVGLLVDEATVTIENINWHLEQGKPVEQAILDGAAQIVTPAFVSLLCICIVFVPM
                     FFLEGVSRFLFVPMAEAVMFAMVSSFILSRTLVPTMANYFLKPHTEGEHGHVSRNPLV
                     RFQQGFEARFERFRGAYKNLLTLAVNHSRLFMAGFMAVVLLSFLLVPLLGQNFFPAVD
                     SGQILMHARVPVGTRIEDTANRFANIQAAIKQIIPADEILTIVDNIGMPISSINLTYN
                     NTGVMGSQDGDIQIALKKHHAPTADYVRTMREVLPKQFPDTTFSFPPADIVSQILNFG
                     SPAPIDIQIRGGKLADNFDYANRLLAKVRSVPGVVDARIQQSRSRPAFDINVDKTRAQ
                     EIGISTRDVTNSMVINMAGSSQVSPTYWLNPANGVTYSIVLQTPQYHLDSLSALANLP
                     ISGSDGPVQTLGGLATFQRNVGNAVVSQYDIQPMVQVHASTQGRDLGAVAKDIRQILE
                     DTKDQVPKASTVVMLGQAYTMDRAFTGLFFGLLGAIVLVYLLIVVNLQSWTDPFVIIT
                     ALPAALAGIVWMLFATYTPLSVPALTGAIMCMGVATANSVLVISFARERLAELQDATA
                     AAIDAGFVRFRPVLMTALAMVIGMTPMALGLGEGGEQNAPLGRAVIGGLFFATVATLL
                     FVPVVFSAIHRKTYPGPVSSTNGESHE"
     gene            5775..6941
                     /locus_tag="VNZ84_03985"
     CDS             5775..6941
                     /locus_tag="VNZ84_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU66146.1"
                     /translation="MSNQVTGMSRGFKLSGLLLVLAAIGIVIVGVTSRANNQDKLKAW
                     TEKQAVATVRANPPEHGAAAGNLILPGRFEAYARAPIYARVSGYLKSWKVDIGGSVKS
                     GQLLAEIETPDLDQELLQAKADLASAQANVALAETTAKRWQEMLKTQSIARQEVDEKV
                     GDYQSKQAIVKAAEANVNRLLALKSFARILAPFDGSVTARNTDTGALINAGSSSATVL
                     PLFEVSNTRKLRLYINVPQNYVGQIKPGGKAKFTVPEAPGAEFEATIQSTSGAINASS
                     GTTLVQLLVENASGRFLPGGFANVNLLFNDVVERLSIPASALIFDQNGLKVATVGHDQ
                     KVTLQTITIARDLGKTLEVQSGLNSHDQVIENPPDGVSDGDVVNLATADVKPKP"
     gene            complement(6964..8499)
                     /locus_tag="VNZ84_03990"
     CDS             complement(6964..8499)
                     /locus_tag="VNZ84_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWU66147.1"
                     /translation="MATHHLPAMPAQAFKTNLLSGLTVALAMVPEAIAFALIAHVSPL
                     TGLYAAVIVSFITSAFGGRPGMISGAAGALAVVMVALVVQHGVQYLFATIVLMGLLQI
                     LFAVAMLGKFIRMVPHPVMLGFVNGLAIVIFLAQFNHFKTLQPDGSSSWMTGAPLYIM
                     LGLIAITMAIIYLLPKLTRAIPSTLAAIVVVSVSVIYFGLETKTVGDLGSIGGGLPSF
                     HLPEVPWTWETLTIIFPYALILAAVGLIETLLTLNLIDEMTETRGRPNRESLAQGAAN
                     IVTALFGGMGGCAMIGQSMINIGNGALQRLSGIATSVFLLSFILFASAWIERIPLAAL
                     IGVMFVVAEKTFEWGTLNLFGKVPKKDVFVGLLVGGVTIVADLAIAVIAGVVVSALVF
                     AWEHAKQMSVRSETDTEGRTVHQLSGTLFFASVSSFQALFQPAHDTDQVVIDFKQARV
                     ADHSALMAIDSLAAKYRAAGKQLYLRHLSPDCQALLTNAAAMIEINLLEDPKYRVPDN
                     ALD"
     gene            complement(8686..9804)
                     /locus_tag="VNZ84_03995"
     CDS             complement(8686..9804)
                     /locus_tag="VNZ84_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWU66148.1"
                     /translation="MVLHWWSTLRGIPFFKEPLFIKLLVVLATVIILHAVVRFLLYRL
                     MRVATISHSYWDDVLTYGAMKPLPAAIWLWGVSHMAKMIARTYQFDDWLEGIVQARNI
                     LALILVGWFFWRMLDEIRQQFLVRTTRTDSQADQTTVDALCKLGYLSVTIITLITLLQ
                     TLGVSISGLLAAGGIGGIAIGFAAKDILANFFGGLTIYLDRPFSVGDWIRSPDKDIEG
                     TVEMISWRHTRIRRFNKNPIYVPNMLFTTIAVENPSRMSHRRIREVIGIRYSDLDKMT
                     AIVNDVRHMLRTHPDIEQTQTLIVNFLTFNASSLDFMIYTFTKTRVWVDYHHIKQDVL
                     LKVADIIRQHGAKIALPTQTIHLSQDSVETADASTVPE"
     gene            complement(9953..10324)
                     /locus_tag="VNZ84_04000"
     CDS             complement(9953..10324)
                     /locus_tag="VNZ84_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HWU66149.1"
                     /translation="MSYLDDPRVYFAAERTLLAWQRSAIALMGLGFVIERFGLFIRLI
                     AHDHPASAHQSALSLYIGVGFILIGASTALLSAIQFLLFCKSLSPQETPRGHIAWLPP
                     AVNLFLAIAAISLSGWLLLAD"
     gene            complement(10342..12561)
                     /locus_tag="VNZ84_04005"
     CDS             complement(10342..12561)
                     /locus_tag="VNZ84_04005"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWU66150.1"
                     /translation="MVSVHRSTHLPTAVDPQAFGSWMASLPQRFTADELLHVQQAGDM
                     ATALYAEHHALNGQPLLQHVLGTASILCEMNMDADSLVAAILHAVPDILADWTETLSA
                     RFSESVIHLIDGISRMSQIQALSEVDGLHAGHKNNDRAQQLEALRKMLLAMVQDIRVV
                     LIKLAERTQLLRHLSGATPEQQVQIARENQHIFAPLANRLGVWQLKWELEDLSLRYLE
                     PQLYKEVAKMLDERRIDREQYITEVVAQLQQELVQAGIQGEVSGRPKHIYSIVKKMKS
                     KHLQFNQLYDVRAVRIMVHDIKDCYAALGLVHHLWQPIPGEFDDYIARPKSNNYRSLH
                     TAVSGPRGLALEVQIRTFEMHQHSELGVAAHWRYKEGGKSDAKFDEKIAWLRQILSWK
                     DEVADHGDLLEQFNSELFQDKVYVLTPQGKVIDLPRGATPIDFAYALHTDLGHRTKGA
                     KINGQIVPLHTRLENGQRVEILTSKQGAPSRDWLNPALGFLQSASARAKVRHWFKYQH
                     FEENVAQGRAKLDKELHRAGAGSLPLERISQKLQFQKLEDCLAAIGRGDISEHQIAIT
                     IQGELAPVEPAPAPPLVKRPTASQVNHAGVTIEGIGNVQTTMARCCKPVSPEPIIGYL
                     TRNHSVTIHRQACTFITRLADARQNRLLVAQWGDRQPGLSEVDIVLEANDRHGLLRDI
                     SELFAREKINTTKANTLSRNHVALMQFTVELSDIDQLQHLLIRLQQVPGVISAQRKT"
BASE COUNT         2870 a         3203 c         3509 g         3047 t
ORIGIN      
        1 gtgaatatga ggctgccgtg gcccaggtag aaaaaatcaa acttgacctc gaacgcaccg
       61 tggtacgttc gcctgtggat ggctgggtgt ctaacctgct ggtgcgccca ggggattttg
      121 cccaggtggg tgcggccaaa ctggcagtga tcgatcagca ctcattctgg gtctatggct
      181 attttgaaga acataaactg agcatgatca atgtggggga tcaggccgag attcaactgc
      241 tgggtagtca tactacgctc aaaggccatg tggaaagcat cgcccatggc attaccgacc
      301 gtgataaccc gaccgatatc cggttgctgg ccaatgtgaa cccaaccttt aactgggtac
      361 ggctggcgca gcgcatcccg gtgcgtatcc acctcgacca gattccggct gccatgccgc
      421 tggttgccgg gatgacgtgt tcggtggtga tccggcacgg ataggctgag gtgcatgcaa
      481 cagaatcagg cagtctctgt taagatggtt attcattgat cacaatctaa gcgcatgctt
      541 acagaaaaac caccccagag cctgttgtcc ctgctgattt ctatcattgc ctacctggtg
      601 ttagcgcaag ccgacaaaat tatgcatatt ggccagccgg aatcgctggt gatttccatc
      661 ctctttgtat tactgattat taacctggcg tttcagttgt ctttccaggt ggagtatgtg
      721 gcagagcggc tcaaggagcc ttatggcacc atgatcctga ccatcagtgc cgtgattatt
      781 gaggtggtga ttattgttat gatgcttagt catacctcat cgaccaccct ggccagggat
      841 gcgatttact cggccgtgat gctggatatc aacggaattc tcggcctgtg tgcaatcgta
      901 ggtggcatca aatatggcga ggtggagtat aacgttaact ccggtaacac ctatattgtc
      961 atgatcatga ccgcgcttgg gatttccatg gcgatgccgg cctttttgcc acctgagcat
     1021 tggcggctct actcgatgtt taccattgtc accatgagtg tgttttatgc attgttttta
     1081 aagatgcaaa ccggccgcca tcgtaactat ttcagttacc gcgaccagca tcaggccaat
     1141 ctgctagcgg gcatgccgaa agcgattagt gccaatgtca aattttccat tctttacatc
     1201 ctggccggca tcgttacgat tggtttcctg tctgaggaaa tgagcaagca tatggatgcg
     1261 gggttaaccg gtagcggagt accgccgatc gtcgccgcga ttgtggtcgc gattatcgct
     1321 gccagcccgg aagtgctgac ggccttgaaa gccgcagtga atgaccgtat gcaaacggtg
     1381 gtcaatattg cgctgggggc tacgctgtct accgtgatcc tgaccgtgcc ggtgattgag
     1441 gcgattgcct tgattgaaga ccggcctttg attgtggggt tgtcaccgac gcagatggtg
     1501 atgacctttt taacgctgat tgtggccaca atcaacttgc atgacggtga aaccaacgcg
     1561 attgagggca tgacacactt tgtattgttt atggcatttg ccatgctggc cgcattgggc
     1621 atgtagtttt tgattaagta aaagaattaa ccttgtatgc ataccgttct tgagttgata
     1681 cagcaatatg ggttgctgat tgtgtttgcc agcgtatttc tggaacagat gggtttgcct
     1741 ctccctgcct atcccacgct gttgctggcg ggtgtgctca tcggcaatgg ccagtactct
     1801 tgggcggcca tgttgctggt ggcgattgtt gcggcgctgg cagcagattc catctggtat
     1861 cgcgccgggc gcaaatatgg caaacgtgtc atgggcaagc tctgcaaaat ttccttatca
     1921 cctgatacct gtgtacggca aaccgaggcc ttgtacctca agtttgggcc gcccgccttg
     1981 ctggtatgca agtttattcc cggttttgct tctatttcga gcgcgttggc aggctcttct
     2041 ggcacgcgtt actggctgtt tgccttgatg gatggcctgg gtgccttact ctgggcgggt
     2101 tcggcgctat ggctgggtta tttattcagt tcggccatcg atgagctgat gctcacgctg
     2161 gttgaaatgg gtaaatgggg caccttgctg gtgctggtgt tgctggtcgc gtttattgct
     2221 tccaagtggt gggacaggca gcgctttatc aaaagcctgc gcatggcacg tatcagtgta
     2281 gatgaattaa ataccatgct taataacggc gctgcgcccg tgattctcga tacgcgtgca
     2341 aaacacctga ttgatgatgg ctggattcca ggtgctcaat ttgtcacgct ggaagatgta
     2401 gaccaattgg tgctggagat cgatgaagat gccccggtgg tgttgtattg ttcatgtccg
     2461 aatgaagtga cggcggccaa agtagccaaa aagctgattg gtcgtggcta tcgcaatatc
     2521 cgcccgttga ctggtggaat tgatgcctgg cgggatgccg ggtttgaagt gacgatgccg
     2581 gaaaaagaag ccatgacgaa ggaagcgcat gtttaacgtc gttggtattg ccctcaagcg
     2641 tccatacact tttgtggtca tggcgctgct gattttaatt gtcggcacct tgtctgccat
     2701 ccgtagcccg gtcgatattt tccctgaaat ccgtatcccg attgtggccg tggcctggca
     2761 atataccggc ttgccgccag atgatatggc cggacgcatc accacgctgt atcagcgtag
     2821 tttaacgaca acggtcaatg atattgagca tattgaagcc aactcctata tcggtttcgc
     2881 catcgttaaa atctttttcc atcctggcgt caatattgcg acggccaatg cccaggtgac
     2941 ggcgatttcg caaacggtga ccaggcaaat gccaaccggc accatgccgc cgttgatcct
     3001 caattacaac gcatccacgg tacccatctt gcagattgcg ctgtcaggca aggggctgac
     3061 tgagcagaat ttggctgacc ttggcctcaa tgctgtgcgc ttacggttgg tgacagtgcc
     3121 gggtgctggc gtgcctttcc cttatggcgg caaaagccgg caaatccaga tcgacctcaa
     3181 tcctgcggcg ttgcaggcac gtggtttgtc tgcacaggat atttccaatg cgctggcagc
     3241 ccataacatg attgtgccgg tgggcacaca gaaaatcggc agtattgaat acacactcaa
     3301 cctgaataac gcggcaaccg cgattgacga tctggccgcg attccaatca aggtggtcaa
     3361 tggggccacg gtgtatatgc gggatattgc ccaggtgcat gatggcagtg cgccacaaac
     3421 caatgtggtc catgtggatg gcaaccgctc tgtgctcatg acgatcctaa aaaatggtgc
     3481 catttctacc ctggcgattg tggaagggat caggagcaag ctggaggaaa tcaaacccag
     3541 cctgccggat gcgttggtgg cgaccccaat caatgatcag tcgctttttg tacgcgccgc
     3601 catcgaaggc gtcgtgtttg aaggcgtgct ggcagccata ttgaccagtt tgatgatttt
     3661 gctgtttttg ggcagctggc gctcgtcact gattattgcc acctctattc cgctttctgt
     3721 gctgggcgca atcatgggcc tttcgctgat cggtgaaacg ctcaatatca tgaccttggg
     3781 tgggctggcg ctggcggtgg gtttgctggt ggatgaagcc accgttacca tagaaaacat
     3841 caactggcat ttggagcaag gcaaaccggt agagcaggcg attttggatg gcgccgcaca
     3901 gatcgtgact ccggcttttg tgtccttgtt gtgtatttgc attgtgtttg tgcccatgtt
     3961 cttcctcgaa ggcgtctcgc gtttcctgtt cgtgcccatg gctgaagcgg tgatgtttgc
     4021 catggtgagc tcgtttattt tgtcgcgcac gctcgtgcca accatggcca attatttcct
     4081 taaaccacat actgaaggag aacatggcca tgtcagccgc aatccgctgg tacgcttcca
     4141 gcaggggttt gaggcacgct ttgagcggtt ccgaggggct tacaaaaacc tcctcacttt
     4201 ggctgttaac catagccgcc tgtttatggc tggtttcatg gcggtggtgc tgttgtcgtt
     4261 cctgctggtg cctttgctgg gacaaaactt ctttccggct gtggatagcg ggcaaatcct
     4321 catgcatgcg cgcgtgcctg tggggacacg tatcgaagac acggccaacc gatttgccaa
     4381 tattcaggca gcgattaaac agatcattcc tgcggatgaa atcctgacca tcgttgataa
     4441 catcggcatg ccgatcagca gcattaacct gacctataac aacactgggg tcatgggttc
     4501 gcaagatggg gatatccaga ttgcgctcaa aaaacatcat gcgccgaccg cagactacgt
     4561 gcgcaccatg cgtgaagtgt tgcccaagca gtttccggat accacattct catttcctcc
     4621 ggcagatatc gtcagccaga tcctgaattt tggctccccg gcgccgattg atatccagat
     4681 ccgtggcggt aaactggctg ataattttga ctatgccaac cgcttgctgg cgaaagtgcg
     4741 cagtgtgccc ggcgtggtcg atgcccgtat ccagcagtca cgtagccggc cagcctttga
     4801 tatcaatgtg gacaaaaccc gtgcgcagga aatcggcatc agcacacggg atgtgaccaa
     4861 cagcatggtc atcaatatgg cgggcagttc gcaggtatcg cctacctact ggctgaaccc
     4921 ggcgaatggg gtgacctatt cgattgtcct gcaaacgccg caatatcatc tggatagcct
     4981 gagcgcgctg gccaatctgc ctatcagcgg cagtgacggc ccggtacaaa ccttgggtgg
     5041 cttggcaact tttcagcgca atgtcggcaa tgctgtggtc agccagtatg atatccagcc
     5101 catggtgcaa gtccatgcca gcacacaggg cagggatttg ggcgccgtgg caaaagatat
     5161 ccgccaaatt ctggaagaca ccaaagacca ggtgcccaaa gcctctacgg tagtgatgct
     5221 gggccaagcc tataccatgg atcgtgcctt caccggcctg ttcttcggac tattaggcgc
     5281 gatcgtgctg gtgtatttgt taatcgtggt caacctgcaa tcgtggaccg atccattcgt
     5341 tattattacg gccttacctg ctgcactggc tggcatcgtc tggatgctct ttgcaaccta
     5401 cacgccattg tccgtacctg cattgacggg ggccattatg tgcatggggg tggcgaccgc
     5461 taacagtgtg ctggtgatca gctttgcgcg cgaacgactt gctgagttgc aagatgcgac
     5521 ggcggcagcc attgatgcgg gctttgtacg cttccggccg gtgctgatga ccgcgctggc
     5581 aatggtcatc ggtatgacgc cgatggccct gggcttgggc gagggtggcg agcaaaatgc
     5641 gcccttggga cgtgccgtga ttggcggtct gttctttgcc actgtcgcga ccttattgtt
     5701 tgtaccagtt gtcttcagtg ccattcatcg caaaacgtat cctggccctg tcagcagcac
     5761 taacggagag tcacatgagt aatcaggtaa cggggatgtc ccgtggattt aagctaagtg
     5821 gtttgctgct tgtattggct gccattggca ttgtcatcgt gggcgttacc agccgtgcaa
     5881 acaaccagga taaactcaag gcctggacag agaaacaagc ggtcgccacc gtaagagcca
     5941 acccgcctga gcatggcgca gcagcaggga acctgattct gcctggcagg tttgaagcct
     6001 atgcacgtgc accgatttat gcacgggtga gcggctattt gaaaagctgg aaagtggata
     6061 ttggcggcag tgtgaaaagt ggccagttgc tggcagagat tgaaacgcct gacctggatc
     6121 aggagctatt acaggccaag gctgaccttg ccagtgccca agcgaatgtc gccttggcag
     6181 agaccaccgc caagcgctgg caggaaatgt tgaaaaccca atcgattgcg agacaggaag
     6241 tcgatgagaa ggtcggggat taccaaagca aacaggcgat cgtcaaggca gccgaggcca
     6301 atgtgaaccg gctgcttgcc ctgaagagtt ttgcccgtat cctggcgcca tttgatggtt
     6361 cagtgactgc gcgaaatacc gataccggcg ctttaatcaa tgctggtagc agcagtgcta
     6421 ccgtattgcc actgtttgaa gtgtccaata cgcgcaagtt gcgtttatat attaatgtgc
     6481 cgcaaaacta tgtcggccag atcaagccgg gtggcaaagc caagtttacc gtgccagaag
     6541 cgccgggtgc cgaattcgag gcgaccatac agtctacttc aggcgcgatc aatgcgtctt
     6601 ctggcacaac gctggtgcag ttactggtag agaatgcgtc cgggcgcttt ttgccagggg
     6661 gctttgccaa tgtcaatttg ctctttaatg atgttgtcga gcgcctgagt attcctgcca
     6721 gtgcactgat ttttgaccag aacggtctta aagtggccac tgttggtcat gatcagaagg
     6781 tgactctgca aacgatcacc attgcccgcg acctggggaa aacactggaa gtgcaatccg
     6841 gattgaacag ccatgatcag gtcattgaga acccgccaga tggcgtaagc gacggcgatg
     6901 tcgtcaatct tgccacggcg gatgttaaac ctaaacctta atgcctaagc gtgtcaggca
     6961 gccctagtcg agtgcattgt ctggcacgcg atatttggga tcttccagta ggttgatctc
     7021 tatcatggca gcagcattcg tcagtagggc ctggcagtcc ggactcaggt gacgaagata
     7081 caactgcttg ccggcggcac ggtatttggc tgccaggcta tcgatcgcca tcagtgcaga
     7141 gtggtcagca acacgtgcct gtttgaagtc aatcacaact tggtctgtat cgtgcgcagg
     7201 ctgaaacaag gcctggaagc tgctgacaga agcaaaaaac aaggtgccac tgagttgatg
     7261 cacggtgcgg ccttccgtat cagtctctga gcgcactgac atctgttttg cgtgctccca
     7321 ggcaaatacc agcgcagaca ccaccacccc ggcaattact gcgatggcca ggtccgccac
     7381 aatagtgacc ccgccaacca gcaagccaac aaacacatct tttttaggca ccttgccaaa
     7441 caggttaagt gtgccccatt caaaggtttt ttcagccacc acaaacatga ccccgatgag
     7501 ggcggccagt ggaatgcgct caatccaggc cgaggcaaac aaaataaacg acagcaagaa
     7561 tacagacgta gcgatgccag acaagcgttg caaggcaccg ttgccgatat tgatcatgct
     7621 ttggcctatc atggcacagc cgcccatgcc accaaacagc gccgtgacaa tattggccgc
     7681 accttgtgcc agggattcgc ggttaggacg gcctctggtc tcagtcattt catcaatcag
     7741 gttcagggtc agcagggttt caatcaatcc caccgcagcc aggattagcg cgtaggggaa
     7801 gataatggtc agggtttccc aggtccatgg cacttcaggc aggtgaaagc tcggcaaacc
     7861 gccaccaata gaccccaggt caccgacggt cttggtttcc agcccaaagt agatcacgct
     7921 gacggacacc acgacgatag ccgccagggt ggaaggaatg gcacgggtaa gcttgggtag
     7981 caggtaaatg atggccatgg tgatggcgat cagtcccagc atgatgtaga gcggcgcacc
     8041 cgtcatccag ctgctgctgc catctggctg taaggtttta aagtgattga actgcgccag
     8101 aaaaatcaca atggccaggc cattcacaaa gcccagcatg accgggtgcg ggaccatgcg
     8161 gatgaatttg cctaacatgg cgacggcaaa caggatttgc aacagcccca tgaggacaat
     8221 ggtggcaaac aggtattgca cgccatgttg tacgaccaga gccaccatga ccaccgcgag
     8281 tgcgccggcg gcaccagaga tcatgccagg ccgcccgcca aaggctgagg taataaaaga
     8341 cacaatcacg gcggcgtata aacctgtcag cggtgaaaca tgggcaatca gggcaaaagc
     8401 aatcgcttct ggcaccatgg ccagtgcgac ggttagcccg ctgagcagat tggttttgaa
     8461 tgcctgtgca ggcatggctg gcaaatggtg cgtagccatc atcactctcc ggaaattggc
     8521 agggctgcag gtatcgaata tctgggcaac tgcaaagggt gggttcaatc agtggcagac
     8581 agggatgtta cccgtctgta tgttagggtt cactcgcgcg acacatggtt tgttgtcaag
     8641 gtcggcgtat acaaaccatg cgtttattat agcatgtgcc tgttatcact ctggtacggt
     8701 gctggcgtcc gctgtctcca cagaatcttg cgaaagatgt atggtttggg tggggagtgc
     8761 gatttttgca ccgtgctggc ggatgatgtc agcaactttc aagagcacat cctgcttgat
     8821 atggtgatag tccacccata cccgggtttt tgtgaaggtg taaatcatga agtccagcga
     8881 ggaggcgttg aacgtcagga aattcacaat cagggtctgg gtttgttcaa tgtcgggatg
     8941 cgtacgtagc atatggcgta catcattgac aatggccgtc attttgtcca aatcgctgta
     9001 gcgaatgccg atgacctcgc gaattctgcg atggctcatg cgagaggggt tctcgacggc
     9061 aatggtggta aacagcatgt taggcacata aatcggattt ttgttgaagc ggcggatacg
     9121 ggtatgacgc cagctaatca tctcaaccgt gccttcgatg tctttgtctg gcgagcgtat
     9181 ccagtcgccc acactaaagg ggcgatccag atagatggtc aggccgccaa aaaagttggc
     9241 cagaatatct ttagcggcaa agccaatcgc gataccaccg atgccgcctg ccgccagcag
     9301 gccagaaatg ctgaccccca gggtttgcaa caaagtgatc agggtgatga tggtgactga
     9361 gaggtagccg agtttgcata aagcatctac ggtggtttgg tcggcttgcg agtccgtacg
     9421 cgtagtgcgc accaaaaatt gctgtcggat ttcatcaagc atgcgccaga aaaaccagcc
     9481 aaccagtatc aatgcgagga tgttgcgtgc ctggacaatc ccctctaacc aatcgtcgaa
     9541 ttgataagtg cgcgcaatca tttttgccat atggctgacg ccccacaacc agatggcggc
     9601 aggtaaaggt ttcatggcgc cgtaggtcag cacatcatcc cagtaactgt ggctgattgt
     9661 ggcaacccgc atgagacgat acaacaggaa tcgtacgaca gcatgcagaa tgatgactgt
     9721 ggctaatacc accagcagtt tgataaataa gggttcttta aaaaatggga tcccgcgcaa
     9781 agtactccac caatgcaaaa ccatgtcctc ctaatcaccg gcagagtgcc gactacagat
     9841 caaaaaggcg catactaaag caatttgctg ccagggtcga atgtgttgct gacaggcagt
     9901 ctggacaatt gccgtaaatc tgggcaagtg taggctgcgt tatgggggag tgttaatcgg
     9961 ccagcaataa ccagccactc agtgaaatgg ccgcaatcgc cagaaacagg ttgaccgccg
    10021 gtggtaacca ggcaatgtgt ccgcgaggcg tttcctgagg actcaaagat ttgcagaata
    10081 acaggaattg tatggcggac aacaatgcag tgctggcgcc gattaaaatg aatcccacgc
    10141 cgatatacag ggaaagtgca gactggtggg ctgatgccgg atggtcgtga gcgattaacc
    10201 ggataaatag cccaaatcgt tcaatcacaa agcccagccc catgagcgca atcgctgagc
    10261 gttgccaggc cagcaaagtg cgctcggcag caaaatacac acgcggatca tccaggtaag
    10321 acatatggcc tctttggtct gtcaggtttt tcgttgtgcc gaaattacgc ctggcacctg
    10381 ttgcaaacgt atcagtaagt gctgtaactg gtcaatatca ctcagttcta cagtgaattg
    10441 catcagtgcc acatggttgc gactgagcgt gttggctttg gtcgtgttga tcttttcgcg
    10501 tgcaaacagc tcgctgatat cacgcagcag gccatgtctg tcgttggctt ccagcacaat
    10561 atccacttct gataagcccg gttgacggtc accccattgt gccaccagca gccggttctg
    10621 gcgtgcatct gccagccgtg tgataaaagt acaggcctgg cgatgtatgg tgacactgtg
    10681 gttgcgcgtc aggtagccga tgataggctc gggtgacact ggtttgcagc atctggccat
    10741 ggtggtttgc acgttgccta tgccttcaat cgtgacgcct gcatggttga cctgcgaggc
    10801 cgttgggcgt ttgaccagtg gcggtgctgg cgcaggttcg acaggtgcga gttcgccctg
    10861 aatggtaatg gcaatctggt gttcgctgat atcgccacgg ccaatggccg ccaggcaatc
    10921 ttccagtttc tggaattgca gcttttgcga aatgcgctct agcggcaggc tgcctgcacc
    10981 tgctctatgc agttctttat ccagtttggc acgcccctgg gcgacatttt cctcaaaatg
    11041 ctggtacttg aaccaatgcc tgactttagc gcgggcgcta gcgctttgca ggaaacccaa
    11101 cgccggattc agccagtcgc ggctgggtgc gccttgcttg gacgtgagga tttcgacgcg
    11161 ctgcccattt tccagccgtg tgtgtaacgg cacaatctgg ccattgattt ttgcgccttt
    11221 ggttctatga ccgaggtcgg tgtgcaaggc gtaggcaaaa tcaatcggtg tcgccccacg
    11281 aggcaaatca atgaccttgc cctgcggcgt tagcacatag actttgtctt gaaacagttc
    11341 gctattgaat tgttccagca agtcgccgtg atctgccacc tcatctttcc atgagagtat
    11401 ctggcgcaac caggcgattt tttcatcaaa ttttgcgtca gatttgccac cttctttata
    11461 gcgccagtgc gcggctacgc ccaactcgga gtgctggtgc atttcaaacg tacggatttg
    11521 cacctccagc gccaggccac gcggaccact gaccgccgtg tgcagtgagc ggtaattatt
    11581 gctcttcggg cgggcaatat agtcgtcaaa ctctcctgga atcggttgcc acaagtggtg
    11641 taccaagccc aaggcagcat agcaatcctt gatgtcatgc accatgatgc gcacggcgcg
    11701 cacatcgtac agctggttaa actgcagatg cttgctcttc atttttttga caatgctata
    11761 aatgtgcttg ggacgtccgc tcacctcacc ctggatgcca gcttgcacca gctcctgctg
    11821 caactgggcg accacctcgg taatgtattg ctcgcggtct atgcgccgtt cgtcaagcat
    11881 cttcgccact tctttgtaga gctgcggttc taggtagcgt aacgataaat cttcaagctc
    11941 ccatttcaac tgccacacgc ccaaacggtt ggccaatggg gcaaaaatat gctggttctc
    12001 acgtgcaatc tgtacttgct gctctggggt ggcaccagat aaatgacgta gcaattgggt
    12061 gcgctcagcc agcttgatca ataccacccg aatatcttgc accatcgcca acagcatttt
    12121 gcgcagggct tccagctgtt gcgcacggtc attgtttttg tgaccggcat gcaagccatc
    12181 cacttcactc aaggcctgga tttgagacat gcgtgagatg ccatcaatca aatggatgac
    12241 agactcgcta aaacgggcgg acagtgtttc cgtccagtca gccaaaatat ctggcacggc
    12301 atgtaatatg gcggccacca agctgtcggc atccatattc atctcgcaca ggatggaggc
    12361 agttcccagc acgtgttgca ataaaggctg gccgttgagg gcgtggtgtt cagcatacag
    12421 ggcggttgcc atgtcaccag cttgttggac gtgtaacaac tcatcggcag taaaccgctg
    12481 gggcagggag gccatccacg aaccgaatgc ttgtgggtcc actgcggttg gaagatgagt
    12541 agagcggtgt actgaaacca tgcagtatta tcgctgctat tgtcagccag cgtaaagaca
    12601 aaacgcaccc tcgcttaaag caagggtgc
//
LOCUS       DATMSK010000116         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_314705,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000116 DATMSK010000000
VERSION     DATMSK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314705"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..983)
                     /locus_tag="VNZ84_04010"
     CDS             complement(297..983)
                     /locus_tag="VNZ84_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HWU66151.1"
                     /translation="MHKKILVSLLALLTPVAAMAEAGDWVVRARAVNVSPNEDSKLGR
                     TVNSNVGNVMSPGAKLEVDSNTIPELDISYYFTKHIAAELILALGTRHDVSVHGDSAG
                     VIGNQKLGSVNLLPPTLTVQWHFRPDQTFDPYVGAGLNYTAMLDRNLQFSSGATDGTK
                     IKIDRDSVGAALQAGFDINLEGGWLINADVKYLWIDTDVKMRNGNQWTKIDSLDINPW
                     VVGIGFGKKF"
     gene            1233..2153
                     /locus_tag="VNZ84_04015"
     CDS             1233..2153
                     /locus_tag="VNZ84_04015"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWU66152.1"
                     /translation="MSMADRDGLIWYDGKMVPWREATTHVLTHTLHYGMGVFEGVRAY
                     KTDKGTAIFRLKEHTDRLFRSAHILGMKMPFDKATVVEAQKAAVRENNLESAYMRPMA
                     FYGAEAMGISAKTLSTHLIVAAWKWGAYMGEEALERGIRVKTSSFSRHHVNITMCKAK
                     ANGNYMNSILAHQEAAADGYDEALLLDVDGFVAEGSGENIFIVRNGKIYTPDLTSALE
                     GITRDTIVQLAAELGYTVIEKRITRDEVYSADEAFFTGTAAEVTPIRELDNRAIGLGK
                     RGPVTEQLQKMYFDVVTGKSAKHMDWLTIV"
     gene            2172..2366
                     /locus_tag="VNZ84_04020"
     CDS             2172..2366
                     /locus_tag="VNZ84_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HWU66153.1"
                     /translation="MAYEAKEIEVTASDLPLHCPTKEVSLWCSHPRVFLDITASGQAM
                     CPYCGTKYRLKAGEVVAHHH"
     gene            complement(2385..>3124)
                     /gene="murI"
                     /locus_tag="VNZ84_04025"
     CDS             complement(2385..>3124)
                     /gene="murI"
                     /locus_tag="VNZ84_04025"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWU66154.1"
                     /translation="PIHQALPQESLIYAADSMHAPYGSKSQAFIIDRAIQLSDFLISR
                     GAKALVVACNTATAAAISELRSRYAIPIIGMEPAVKPAAAATRSGVIGVLATSGTLQS
                     AQFAALLESYGRQVRVETQACVGLVECIERGELQTAATRQLLEKYIQPLIEAGADTLV
                     LGCTHYPFVRPLIEELAGPEVAIIDTGAAVARQLKYRLQEADLLKDDSYKMPGVTEFW
                     SNADGLSAQSVTSLLWGTPVEVMRLPA"
BASE COUNT          795 a          859 c          748 g          722 t
ORIGIN      
        1 ttatattgtt cgctcactgc actcacattg acgccagaag ttgcgacgct cgccgttgcc
       61 attactacgt ctcccgatca aaaaccgcta aagtaaagag ttgcgattat attctgtaac
      121 agctttacag aacaacataa aaaccctttt tactacatga gttttcacac attcatattc
      181 cagacttgtg cagatttgcc caaagggccc cttgcgtcag gcttttgcaa atcgtgacgg
      241 gcgtcactta aaggcaacac ccgccccgct gatgtcatgc gtgaagtttt tttatattag
      301 aacttcttgc cgaagccaat gccgaccacc catgggttga tgtcgagact gtcaattttg
      361 gtccactgat tgccgttgcg cattttcaca tccgtatcaa tccagaggta tttgacatcg
      421 gcattgatca gccagccacc ttccaggttg atatcaaaac ccgcctgcaa cgctgctcct
      481 acgctatcgc gatcaatctt gatcttggtg ccgtcggtag cacccgaaga aaactgcaga
      541 ttgcgatcca gcatggcggt gtaattcaag ccagcaccga cataaggatc aaatgtctga
      601 tcaggccgga agtgccattg cacggtcaaa gttggcggca acaagttaac cgagcccagc
      661 ttctggttgc caattacgcc agcagaatcc ccatgaacac tcacatcgtg gcgtgtaccc
      721 agtgccagga tcagttcggc agcaatatgc ttggtgaagt agtaagagat gtccagttca
      781 ggaatcgtat tgctatcaac ctcaagcttg gcgcctgggc tcattacatt acctacatta
      841 ctatttactg tgcgccccaa tttactatct tcgttagggc tgacattcac cgcccgggca
      901 cggaccaccc agtcgcccgc ctcggccatc gcagccacgg gtgtcagtaa agccagcaaa
      961 gaaaccagaa tttttttatg catgataatc ccctcttgaa atgaccataa aaagaacatg
     1021 gattaaactt tacacgtatt aaccatattt taaaagtggt ttatacttga ccataatgct
     1081 cggctttagc cgttcgagcg gcagacacca cattcccgac cagtcaatct ttgactacac
     1141 cgctacatag cgccgatttc cgctattcaa atacaagggc ttgcgattac aatgatgctt
     1201 ttaatagctt tagtattgat ggagaactga agatgtcgat ggctgaccgt gatggtctaa
     1261 tttggtatga cggcaaaatg gtgccatggc gtgaggcaac cacgcatgtt ttaacccaca
     1321 cattgcatta tgggatgggg gtttttgaag gcgtgcgcgc atacaaaacc gataaaggca
     1381 cggccatttt ccgcctgaaa gaacatacgg atcgcctgtt tcgttccgcg catattctgg
     1441 gcatgaaaat gcctttcgac aaagcgactg ttgtggaagc gcaaaaagcc gctgtgcgcg
     1501 aaaataatct tgagtcagcc tacatgcgcc cgatggcatt ttacggtgct gaagccatgg
     1561 gcatttcggc caagacactg tctacacacc tgatcgttgc cgcatggaaa tggggtgcct
     1621 acatgggtga agaagccctt gagcgtggca ttcgcgtcaa gacctcgtct ttctcgcgtc
     1681 atcacgtgaa catcaccatg tgtaaagcca aggcaaatgg caactacatg aactccatcc
     1741 tggcacatca ggaagccgcc gctgacggct atgatgaagc cctgctactg gacgtggatg
     1801 gttttgttgc cgaaggttcc ggcgagaata ttttcatcgt acgtaatggc aagatttaca
     1861 cgccagatct gaccagtgcg ctggaaggca ttacccgcga caccatcgtg caactggctg
     1921 ccgagcttgg ttataccgtg attgagaagc gcattacccg cgacgaagtc tactccgctg
     1981 acgaagcgtt cttcactggc accgccgctg aagtcacgcc gattcgcgag ctggataatc
     2041 gcgctatcgg cttgggcaaa cgcggcccgg ttaccgagca actgcagaag atgtattttg
     2101 atgtggttac cggaaaatcc gccaaacaca tggactggct caccatcgtt taaagcaaca
     2161 aagggaacat catggcatac gaagcgaaag agattgaagt gacagccagc gacctgccgc
     2221 tgcactgccc gaccaaggaa gtctcgttat ggtgttccca tccccgcgta tttcttgaca
     2281 tcaccgctag cggtcaagcc atgtgcccct attgcggcac caagtacagg ctgaaagcag
     2341 gcgaagttgt cgcccaccat cactaaaaca atattggcca gtgcttaagc tggcaagcgc
     2401 atcacttcaa cgggtgtgcc ccacaacagg ctggtcactg actgcgctga taacccatcg
     2461 gcgttactcc agaactctgt tacgccaggc atcttgtaag agtcatcttt gagcaggtct
     2521 gcttcttgca gacggtactt caactgccgc gcaactgccg ctccggtatc gataatggcc
     2581 acctccggcc cggcaagctc ttcaatcagc ggtcgaacaa aaggataatg cgtacaccca
     2641 agcaccaggg tatctgcacc agcctcgatc aaaggctgga tatacttttc cagtaattgc
     2701 cgtgtagcag ccgtttgcaa ttccccacgc tcaatacact ccaccaggcc aacacaagcc
     2761 tgcgtttcca cccgcacctg acgaccatag ctctcaagca aggcagcaaa ctgcgcgctc
     2821 tgcagcgtac cactggtcgc aagcacgccg atcacgcccg agcgtgtggc agcagcagct
     2881 ggcttaaccg cgggctccat gccaataatc gggatcgcat aacggcttct tagctcgctg
     2941 atggccgcag ccgtggccgt attgcatgcc accaccaagg ccttggcgcc acgcgaaatg
     3001 agaaagtccg acaactggat ggcacgatca ataataaatg cctgcgattt gctgccataa
     3061 ggcgcatgca tggaatcagc agcatagatc agcgactcct gcggtagcgc ctggtgaata
     3121 gggg
//
LOCUS       DATMSK010000117         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_385086,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000117 DATMSK010000000
VERSION     DATMSK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385086"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..2508
                     /locus_tag="VNZ84_04030"
     CDS             325..2508
                     /locus_tag="VNZ84_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HWU66155.1"
                     /translation="MEIYTSLTAKPVNSYRPYWAKRFGTAPVLPMSRAEMDALGWDSC
                     DIILVSGDAYIDHPSFGVALIGRLLEAQGFRVGIIAQPDWQSAEPFKALGKPNLYFGV
                     TAGNMDSMVNRYTADRKIRSDDAYTPNAEANKRPDRAVLVYSQRCKEAYSDVPLVIGS
                     IEASLRRIAHYDYWSDKVRRSILIDSKADLLVYGNAERAIVELSHRIARGEALNDIQD
                     IRGTAFIRKQVPDDFVEVPSTRLDRPGVVEKHEDPYAMKMGKGDASCATDDKAAETAP
                     IISLPLPLKAAKVPRDRQVIRMPSYEEVANDPVMYAHASRVLHLEANPGNARALVQKH
                     GNREVWLNPPPIPLSTKEMDYVYDLPYARMPHPAYQGANIPAWEMIRFSVNIMRGCFG
                     GCTFCSITEHEGRIIQSRSEDSILREIEEIRDKVDGFTGVISDLGGPTANMYRLSCKS
                     EEIEKNCRKLSCVYPGVCENLNTDHSALIQLYRKARNLRGVKKILIGSGLRYDLAVKS
                     PEYVKELVQHHVGGYLKIAPEHSEDNVLSKMMKPGMGAYDEFKRMFEKFSAEAGKKQY
                     LIPYFIAAHPGTTDEDMLNLALWLKRNDFRLDQVQTFTPTPMAMATAMYHSGKNPLRK
                     VTADSEPVPVPKTGTIRKLHKAFIRYHDPQNWPMLREALKKMGRADLIGNGKKHLVPA
                     YQPAVLPKIVQSDGSRFQAKPLPKGFNQTKSFASPKKHTGGRHKH"
     gene            complement(2526..3581)
                     /locus_tag="VNZ84_04035"
     CDS             complement(2526..3581)
                     /locus_tag="VNZ84_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HWU66156.1"
                     /translation="MRKKERLIVLFDGTWNDPEDLTNVYQLSTLIEEYDGDIYQRFFY
                     EPGVGTATGDKLRGGLFGYGLSENLLKGYDWLAKHFQEGDDIWVFGFSRGAYTARSMV
                     GLIRKCGLLKTSTPDLLQRAEKLYRDKSAHPDSAQCQAFRQTYSKEVRIHFLGVWDTV
                     GALGIPDTMISEYGFYAWHDTELSKIVSYAYHAMAIDEHREAYKVAMWTNDGGSKKPE
                     NIAVEQRWFIGAHANVGGGYGKDPLAGIALDWMMEKAREAQLKLKPFTAAPDAYLTNP
                     TDSFKAFAYGLYAWFKGVFKKGDGRFYRPYAVAQGQLRAVNVTIDASVAAKWRAQADY
                     RPPTLVSVQIDPTKTAD"
BASE COUNT          874 a          925 c          910 g          890 t
ORIGIN      
        1 ggcaataaga tcttcttgaa taatgcgtaa tgcttcattg ttaatctatg taattattat
       61 ataaaaaata tccccgtgat gctctatggt tatacagggc tttaaccctg gccgcaatcg
      121 taaatggggc atgcaggcga tatgtaagta ttttttaatg cctatcgtct gattcagcac
      181 acaaacagat tttgtgtcca tgtttgtggc tctggctttg tgctgcgcat gttgaattaa
      241 tggtcttttt gttaaaaaaa tgatttcaaa tcaatcgatt aacgcgtaaa atgcgcaact
      301 ctcaagcatt gctggtcacc tcgcgtggaa atctacactt cccttactgc caaacctgtt
      361 aacagctacc gcccttattg ggccaagcgt ttcgggactg cacctgtgtt acccatgagc
      421 cgtgctgaaa tggatgcact aggctgggat agttgcgaca tcattctggt aagtggtgac
      481 gcctatattg accatcctag ctttggcgtg gccttgatcg gacgtttgct agaagctcag
      541 gggttccgcg tcggcatcat tgcgcagcct gactggcagt cggcagaacc attcaaggcg
      601 ttgggtaagc ccaatttgta ctttggggtg acggcaggca atatggatag catggtcaac
      661 cgctatacgg cagaccgtaa aatccgttcc gacgatgcat atacgcccaa tgcagaggct
      721 aacaaacgcc cggaccgtgc tgtgctggtt tattcgcaac gctgcaagga agcttattcc
      781 gatgtgccgc tggtgatcgg cagtatcgag gccagcctgc gccgtatcgc gcattatgat
      841 tactggtcag acaaagtccg gcgctctatc ctgattgatt ccaaagcgga cctgttggta
      901 tatggcaatg ccgaacgtgc catcgtcgag ctctcacacc gtattgcgcg tggggaagcc
      961 cttaacgaca tccaggatat tcgtggcacg gcctttatcc gcaaacaggt gccggatgat
     1021 tttgtagaag tgccgtctac gcgcctcgac agacctggtg tcgttgaaaa acatgaagat
     1081 ccctatgcca tgaaaatggg caagggggat gccagctgtg cgaccgatga caaagcggcc
     1141 gagactgcgc ccatcatcag cctgccatta ccgctcaaag ccgcgaaggt gccacgcgac
     1201 cgccaggtga tccgtatgcc atcgtacgag gaagtggcga atgatcctgt gatgtatgcg
     1261 catgcctcac gcgtgttgca cctggaagcc aaccccggca atgcacgtgc actggtgcaa
     1321 aagcatggca accgtgaagt ctggctcaat ccgccaccga ttccgctttc cactaaagag
     1381 atggattacg tctacgattt gccgtatgca cgtatgccgc atcctgccta tcaaggcgcc
     1441 aatattcctg cctgggaaat gatccgcttc tcagtcaata tcatgcgcgg ttgctttggt
     1501 ggctgtacct tctgttcaat taccgagcat gaaggtcgca ttatccagag ccgctctgaa
     1561 gattccattt tgcgcgagat tgaagaaatc cgtgacaagg tcgatggttt taccggcgtg
     1621 atttcggatc taggcggccc gacagccaat atgtaccggt tgtcttgcaa gtcggaagag
     1681 attgaaaaaa attgccgcaa actctcatgt gtctatcctg gcgtttgtga aaacctcaat
     1741 accgatcatt cggccttgat ccagctttac cgcaaggcgc gtaatctgcg cggcgtgaag
     1801 aaaatcctca ttggctcagg actgcgctat gacctggccg tgaagtcccc cgagtatgtg
     1861 aaggagctgg tacagcacca tgtgggaggc tacttgaaaa ttgcgcctga gcactcagaa
     1921 gacaatgtgc tcagcaagat gatgaagcct ggcatggggg cttatgatga gttcaagcgc
     1981 atgtttgaaa agttcagcgc cgaagccggc aagaaacaat acctgatccc gtattttatt
     2041 gcagcccatc ctggcactac ggacgaagat atgctcaacc tggcgctatg gttgaagcgt
     2101 aatgatttca ggctagacca ggtgcaaacg tttacaccaa cgccgatggc gatggcgacc
     2161 gccatgtatc acagtggcaa aaaccctttg cgcaaggtga cagctgattc ggagcccgtg
     2221 ccagtaccta agaccgggac catccgcaaa ctgcataaag cttttattcg ctatcatgat
     2281 ccgcagaact ggcccatgtt gcgtgaggcg ctgaaaaaaa tgggccgtgc agacttgatc
     2341 ggtaatggta aaaagcacct ggtgcctgct taccagccgg cggtcttacc taaaattgtg
     2401 cagtctgatg gtagccgctt ccaggccaag cctttgccta aaggatttaa ccagaccaag
     2461 agctttgcca gcccgaaaaa gcatactggc ggccgccaca agcattaatc ctatcagtat
     2521 cactatcaat cagctgtctt ggtgggatca atctgaacac tgaccagcgt tggcggacga
     2581 taatccgctt gcgcccgcca tttggcggca acactggcat caatcgtgac attgacggcg
     2641 cgtaactggc cttgcgccac tgcgtatggc cggtaaaaac ggccatcccc ttttttgaat
     2701 acgcctttaa accacgcata caagccataa gcgaaggctt taaatgagtc ggtagggttg
     2761 gttaaatagg catccggtgc tgcagtaaaa ggcttaagct ttagttgggc ctcacgtgct
     2821 ttttccatca tccagtccaa tgcaatcccg gccagtggat ctttgccata gccgccgccg
     2881 acattggcgt gggcgccaat gaaccagcgt tgttctactg caatattctc cggttttttg
     2941 ctgccaccgt cattggtcca catggctact ttgtaagcct cacgatgttc atcgatggcc
     3001 atggcgtgat aggcataaga gacaattttg gaaagctccg tatcatgcca ggcgtaaaag
     3061 ccgtactccg aaatcatggt gtcgggaatg cccagcgcac ccacggtgtc ccatacgcct
     3121 aaaaaatgga tacggacctc tttgctgtag gtttgcctga atgcctggca ttgcgcacta
     3181 tcaggatggg cagatttgtc gcggtagagc ttttctgcac gttgtaataa atctggcgta
     3241 ctggttttga gtagcccgca tttccggatc aggccgacca tgctcctggc cgtataggca
     3301 ccgcggctaa aaccgaacac ccagatatca tcgccctcct gaaagtgttt cgccagccag
     3361 tcgtagccct tgagcaggtt ttcactcagt ccatagccaa ataagccacc gcgcaatttg
     3421 tcgccggtgg cggtgcctac accaggttcg tagaaaaaac gctggtaaat atcgccgtcg
     3481 tactcttcaa taagtgtgga taattgatac acattcgtca gatcttcagg atcattccag
     3541 gtgccatcaa acagcacaat caatctttct ttttttctca tgggctgtct ccaggctag
//
LOCUS       DATMSK010000118         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_196781,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000118 DATMSK010000000
VERSION     DATMSK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196781"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VNZ84_04040"
     CDS             <1..1078
                     /locus_tag="VNZ84_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWU66157.1"
                     /translation="FGTQTLKEYKLPFEDQYPSGPFERLDKKLFLFISKCGDVFFVRQ
                     NESMELVKPGKPLVKFIPGGQTSADESSSKQLVYCKELSGVKDSLLIDKILYVAYTAW
                     DEANNGVRLAVSEFEVDVENSEVSFNREIYISRPAIKEPILGHQVGGKLALGENERTL
                     YLSLGDFSKPDRVQDKTTSLGKVVRIDLQRLNAEVYASGFRSPSGGLFYDKESNELWL
                     TDHGPRGGDEINLIKRGKNYGWPIVSYGTIYERDGIGGYYGNKFNSHEGYEKPQMTFV
                     PSIGIGPLVKYPSTGRNDFWDNDLFVAGMGNTTLYRVRKEGTSLVYAEPVLSGYRIRA
                     IQIDQNGTFYLKTDHNQLLISEQE"
     gene            1181..1582
                     /locus_tag="VNZ84_04045"
     CDS             1181..1582
                     /locus_tag="VNZ84_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66158.1"
                     /translation="MKPTKKTSLVALAGALIAIGFEINAATHLRFLSHRAGLLMTLWT
                     VMPYIILWAGKYFFQRCDIQKQWLRFAVFEVLVVTWMYWQTLVMYPDAQDSLIFLFLP
                     LIQSGLNLAIGVLFYWRQSVLVKKGVHDRCD"
     gene            1601..2044
                     /locus_tag="VNZ84_04050"
     CDS             1601..2044
                     /locus_tag="VNZ84_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWU66159.1"
                     /translation="MKTTPVIGLAYQHTFTVPISKTVPALYPEAPEFQVMPVVFATGF
                     MVGLLEWACIKAIHPYLDWPDEQTVGTHINVSHQAPTPPGMTVTVSVALLAIEGRKLV
                     FSVEAHDGIDLIARGTHERFIIQRDRFDAKLSAKLVAHQPPLPHG"
     gene            2078..2401
                     /locus_tag="VNZ84_04055"
     CDS             2078..2401
                     /locus_tag="VNZ84_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HWU66160.1"
                     /translation="MLKTLFLFALTAVAEIVGCYLPYVWLKAGKSAWLLLPAAMSLAL
                     FAWLLTLHPAASGRVYAAYGGVYIGVAIFWLWLVDGIRPTVWDLLGATVAMLGMVIIM
                     FAPRH"
     gene            2403..2813
                     /locus_tag="VNZ84_04060"
     CDS             2403..2813
                     /locus_tag="VNZ84_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66161.1"
                     /translation="MNRILRYGWAFPTTLLGLLFANLLWLTGARAQRVEGVLEVSGGW
                     LAQRLTQRSGFAALTLGHVVLGCSADCLQRLRVHEHVHVRQAERWGFLFVPAYLLAGL
                     WQLVCGRHAYYDNPFERQAFDAESLECTIQPLKD"
     gene            complement(2886..3719)
                     /locus_tag="VNZ84_04065"
     CDS             complement(2886..3719)
                     /locus_tag="VNZ84_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWU66162.1"
                     /translation="MLRILPLNTLFTRAFILITALTLSACAIFGNPNPPDETKGWAEQ
                     RLYAAGQEKMADKNYDKAIEYFQKLEARYPHGIYATQSQLEVAYAYFKKQEPVLCLSA
                     IDRFIKLHPNHPNLDYAYYLKGLATFNERGFMEKYTKQEINDRDPKTLRVSFNAFKEL
                     VERFPTSRYHKDATQRMIYLVNTLAMHEMHVARYYMQRKAYVAALNRARYVLETYPNS
                     SSVEDALVTQISAYDAMDLTDLKNDTLRILKTNYPENPMVTGKINEDEKIWWKFWESL
                     Y"
BASE COUNT          909 a          842 c         1021 g         1057 t
ORIGIN      
        1 attcgggaca caaaccctca aggaatataa attacctttt gaagatcaat atccatccgg
       61 accatttgaa cggctggata aaaagctatt cctgtttatc agcaaatgtg gcgacgtatt
      121 ttttgtccga cagaatgaaa gcatggagtt ggtaaagccc ggcaagccac tggtcaaatt
      181 tattcctggc ggtcaaacat ctgccgatga aagcagcagc aagcagttgg tgtattgcaa
      241 agagctgtcc ggtgttaagg actctttact gatagataag atcctgtacg tagcctatac
      301 ggcttgggat gaggccaata atggcgtcag gctggcggtc agtgagtttg aagtggatgt
      361 cgagaacagc gaggtcagtt tcaatcgcga aatttatatc agccgcccgg ccattaaaga
      421 gccgatcctg gggcatcagg tgggcggtaa attggccttg ggagagaatg agcgtacctt
      481 gtacctgagc ctgggtgact tctccaagcc tgaccgcgtg caggataaaa ccacttcttt
      541 aggcaaggtg gtacgcattg atttgcagcg gctgaatgcc gaagtgtacg cctcggggtt
      601 tcgatcacct tctggcggat tgttttatga caaggaaagt aacgaattat ggctcactga
      661 tcacggccca cgtggtggcg atgaaatcaa cctgattaaa cgtggcaaaa actacggctg
      721 gccgattgtc agttacggta cgatttacga acgtgatggc ataggtggct attacggcaa
      781 caaattcaat agccatgaag gctatgaaaa gccgcaaatg acgtttgtgc catcgattgg
      841 tattggccca ctggtgaagt atccttctac cgggcgcaat gatttctggg ataacgattt
      901 atttgtcgcg ggtatgggca acaccacttt atatcgtgtg cgcaaagagg gcacttcgct
      961 ggtgtacgcc gagccggtac tgagtggata tcgcatccgt gccatccaga ttgaccagaa
     1021 tgggacgttc taccttaaaa cagaccacaa ccagctgctg atctcagaac aagagtagca
     1081 gagcggtaca caggggtggc gagatcaatg ccttgcttgc aagcccaatc tggcatacat
     1141 tggctgactg ttaatgacgc ccaattgatt aagtattttg atgaaaccca cgaaaaaaac
     1201 ttctctggtg gctttagctg gcgccttgat tgcgattggt tttgagatca atgccgccac
     1261 tcatttgcgt ttcctgagcc atcgggccgg actactcatg acgctatgga cagtcatgcc
     1321 ttatatcatc ctgtgggcag gcaaatattt cttccagcgc tgtgatatcc aaaagcagtg
     1381 gttacgcttt gctgtttttg aggtgctggt ggttacatgg atgtattggc aaacgctagt
     1441 gatgtatcct gacgcacaag atagcctgat tttcctattt ttacccctga tacagtcggg
     1501 cttgaacctt gccattggtg tgctgtttta ttggcgtcaa tcggttctgg tcaaaaaagg
     1561 tgtacatgat cgttgtgatt aaagcgcgta aaagcttgcg atgaaaacta caccagtcat
     1621 tgggcttgcc taccaacata cattcactgt ccccatttct aaaaccgttc cagccctcta
     1681 tccggaagcc cctgagttcc aggtcatgcc tgtcgtattt gccactggtt ttatggtcgg
     1741 cttgcttgag tgggcgtgca tcaaggcgat ccatccatac ctggattggc ctgacgaaca
     1801 aacggtaggc acacacatca atgtgagcca ccaggcgcct acgccgccgg gaatgactgt
     1861 cacagtgtcg gtggcgctac ttgcgataga gggccgtaag ttggtatttt cagtagaggc
     1921 gcacgatggc atagacctga ttgcacgtgg tacacacgag cgctttatca ttcagcgtga
     1981 tcgctttgat gccaagctct ccgccaagtt agtggctcat caacctccgt tgcctcacgg
     2041 ataagtgagg ttgtgcacgt catgaaaagg attctttgtg cttaaaaccc tgtttttatt
     2101 tgcgctcacg gcagtggcag aaatcgttgg ctgctatttg ccatatgtct ggctaaaagc
     2161 gggcaaaagt gcctggttgt tattgcctgc cgcgatgagc ctggcattat ttgcctggtt
     2221 gttgactttg catcctgctg cttctggcag ggtttatgcg gcttatggtg gtgtttatat
     2281 tggcgtggct attttctggt tgtggctggt ggatggtata agacctacgg tttgggacct
     2341 gcttggcgca acggtggcga tgctggggat ggtcattatc atgttcgcgc ccagacatta
     2401 aaatgaatcg catattacgt tatggctggg cattcccaac gactttgctt ggtttgcttt
     2461 tcgcaaacct gttatggctg accggcgcac gtgcacagcg cgtagagggc gtgctggaag
     2521 tgtccggcgg ctggctggca caaagactga cgcaacgcag tggttttgct gcattgacgc
     2581 tggggcatgt tgtattaggc tgttcggctg actgtctgca acgcttgcgt gtgcatgaac
     2641 acgtacatgt gcgccaggca gaacggtggg gtttcttatt tgtgcctgct tatttgctgg
     2701 caggactttg gcagttggtg tgtggacgac acgcgtacta tgataatccg tttgagcggc
     2761 aagcttttga cgctgaatca ctcgaatgta ccattcagcc attaaaagac tagaatgcta
     2821 ttcataaaaa aacgccagtg gatactggcg ttttttgtag gcgtttgagg ctggtaaaag
     2881 gggatttaat acaagctttc ccagaatttc caccagatct tttcatcttc attgatcttg
     2941 ccagtcacca tcgggttctc aggatagttt gttttcaaga tgcgcaaagt atcattttta
     3001 agatccgtca aatccatcgc gtcataggca ctgatctggg tgaccagcgc atcctccaca
     3061 gaacttgagt tagggtaagt ttctagtaca tagcgtgcac gattcaatgc agcgacatag
     3121 gctttgcgtt gcatgtagta acgcgcgaca tgcatttcgt gcatggccag agtattgacc
     3181 aggtaaatca tgcgttgagt cgcatcttta tggtaacggc tggtcgggaa gcgttcaacc
     3241 agttccttaa aggcattgaa tgagacacgt aaggttttag ggtcgcggtc attgatttcc
     3301 tgcttggtat atttttccat aaaaccgcgc tcattaaagg tggccaggcc tttgaggtag
     3361 taagcataat caaggttagg gtgattgggg tgcagcttga taaaacggtc aatggcagat
     3421 aaacataaca ctggctcttg ctttttgaag taggcgtaag cgacttcgag ttgtgattgt
     3481 gtcgcataga taccgtgcgg atagcgcgct tccagcttct ggaaatactc aatggccttg
     3541 tcgtagtttt tgtcagccat cttctcctgg cctgccgcgt aaaggcgttg ttctgcccag
     3601 cccttggtct catctggcgg gtttgggttg ccgaaaatgg cacaagcact caatgtcagc
     3661 gcggtgatca aaataaaagc acgcgtgaac agggtgttaa gcggtaaaat gcgaagcatg
     3721 ttgtttaatg ttgttttatt taaggaaact cagggcatta taactgatgc cgtttgaaaa
     3781 tatgaaagaa agcgcctcct tacatctggt cgttcccggc aacctcggc
//
LOCUS       DATMSK010000119         8848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_103120,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000119 DATMSK010000000
VERSION     DATMSK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103120"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4512
                     /locus_tag="VNZ84_04070"
     CDS             <1..4512
                     /locus_tag="VNZ84_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HWU66163.1"
                     /translation="SHDIVAKGLQLLDNLTHRGATGYDPKLGDGAGLLMQMPDTFIRD
                     EAAKLGMTLPAAGQYAVGQAFLPQNADNRAKCEAIFAQIIAEENQTLLGWRDVPVDNS
                     NIADAARAVEPVMRQVFIASTAADSTTFERKCFVIRKRIEHAIRALNLQDKAQFYMPS
                     FSSRTIVYKGMLLANEVGVYYKDLKDERVVSALALVHQRFSTNTFPAWDLAHPFRMIA
                     HNGEINTVQGNVNWMAARHETMKSAVLGEDLEKLWPLIAEGQSDSACFDNCLELLVAG
                     GYSLPHAMMMLIPEAWAGNPLMDEDRRAFYEYHAALMEPWDGPAAVAFTDGKMIGATL
                     DRNGLRPARYLVTEDDIVMMASEMGVITFPQEKIVKKWRLQPGKMLLIDMEQGRIIDD
                     EEVKTELAQAKPYRQWIEQTRFHLTELPSVGSGEVNLNADLLDTQQAFGYSQEDLKFV
                     LQPMLDNGEEGSGSMGNDAALPVLSNKAKTLYNYFKQLFAQVTNPPIDPIREELVMSL
                     VTFIGPKPNLLAIDETNPPLRLESSQPVLSLDGLEKLKQIESLTKGQFKSIVLDLTYE
                     AALGKAGMKQALDALHDKAEKAVQDGYNVLILSDRNVSASRIAIPALLACSATHEFLV
                     KAGLRTSTGLVIDTGSAREVHHFALLAGYGAEAVCPWLVYETMKTMTSDVEKAGKNFV
                     KAIGKGLYKVMSKMGISTYQSYCGAQIFEAIGLNSQFVNEYFTGTVSQIEGIGLEQVS
                     EETVRLHSQAFGTDPVLSNALEAGGEYAFRVRGEEHLWTPQSISKLQNATRTGQYETY
                     KEYAALINDQTRRHMTLRGLFEIKPAGKAIPLDQVESAKEIVKRFATGAMSLGSISTE
                     AHATLAIAMNRIGGKSNTGEGGEDVKRFIPVQVDSSIAKVLGSDLIEADVALKAGDSM
                     RSRIKQVASGRFGVTAEYLSSADQIQIKMAQGAKPGEGGQLPGHKVSPYIAKLRFSVP
                     GVGLISPPPHHDIYSIEDLAQLIHDLKNANPKASISVKLVSETGIGTVAAGVAKAKSD
                     HIVVAGHDGGTGASPISSIKHAGTPWELGLAETQQTLVLNQLRGRVVLQVDGQMKTGR
                     DVLIGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPELRKKFTGQPEHV
                     VNYFFFVAEEVRELMASIGIAKFDDLIGRADLLDMKAGIEHWKINGLDFSKVFHLPEM
                     PASVSRKNNDVQDHGLVKALDNQLIELAKPALEKGEKVSIALPIVNTNRTVGTMLSHE
                     VASRYGHAGLPDDTISVKFTGTSGQSFAAFLAQGITFELTGEGNDYVGKGLCGGRIII
                     KPPATFRGLSYENIIVGNTVMYGATTGQSFFSGVAGERFCVRNSGATAVVEGVGNHGC
                     EYMTGGTVLVLGATGQNFAAGMSGGIAYVYDIDGKFAQRCNMSMVSLDKVEAAEADVG
                     KVQHLGQPDEVILKGLIEQHLALTGSPRAKELLADWPASRAKFVKVFPNEYKRALTEM
                     AAASAKQAA"
     gene            4599..6026
                     /locus_tag="VNZ84_04075"
     CDS             4599..6026
                     /locus_tag="VNZ84_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HWU66164.1"
                     /translation="MGKVTGFMEFDRLAEENLPVKDRVKNYKEFVLHLTDDQAKQQGA
                     RCMDCGIPFCTTGCPINNIIPDWNDLVYHQDWRSAIDVLHSTNNFPDFTGRICPAPCE
                     AACTLNINNDAVGIKSIEHAIIDKAWENGWVTPQVNPNKTGKKIAVVGSGPAGLAAAQ
                     QLARAGHSVTVLEKNDRIGGLLRYGIPDFKLDKSNIDRRITQMEAEGVTFKVNQNVGE
                     NVSAADLERDFDAVILAGGAELPRDLPVPGRELDGVHFAMDFLTPNNKMVAGDTVPNQ
                     IKATGKHVVVIGGGDTGSDCVGTSNRHGAASVTQFELMPQPPEKENKSLVWPNWPLKM
                     RTSSSHEEGADRDWSITTKELIGENGKLTALKGARVEWKDGKMVEVPGSEFTIKADLV
                     FLAMGFVSPVQKVLDAFGVEKDNRGNAKATTDGEGCYKTSKAKVFAAGDIRRGQSLVV
                     WAIREGRQCAREVDAFLMGSSNLPR"
     gene            6200..7264
                     /gene="hemE"
                     /locus_tag="VNZ84_04080"
     CDS             6200..7264
                     /gene="hemE"
                     /locus_tag="VNZ84_04080"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWU66165.1"
                     /translation="MTQLQNDTFLRALMRQPTEYTPVWMMRQAGRYLPEYRESRKTAG
                     SFMQLCQSPSFATEVTLQPLDRYPQLDAAILFSDILTVPDAMGLGLYFEEGEGPKFER
                     TLQAEEDILKLEVPDMTKLQYVFDAVSSIRKALNGRVPLIGFSGSPWTLATYMVEGKG
                     GTDFLNTKKMGYARPELLHRILEITSQTVIQYLNAQIAAGAQAVMIFDSWGGALSHDA
                     YLEFSLHYMQKIVAGLTKTAEGKTVPSIVFTKGGGLWLEQQAAIGADALGLDWTVDLA
                     QARQRVGDQVALQGNMDPAILLSSPAAIEKEVARILASYGHGHGHVFNLGHGITQWTP
                     PEHAACMLETLRKLSPQYHG"
     gene            complement(7309..>8848)
                     /locus_tag="VNZ84_04085"
     CDS             complement(7309..>8848)
                     /locus_tag="VNZ84_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWU66166.1"
                     /translation="NLVWLGITIFWLGAAAVCRPLALPDEGRYVGIAWEMATSGDWLV
                     PHLDGLPYFHKPPLFYWLTAASFKACGPSLLCSRFAPLLSALMMALGLFWFLSRHVDR
                     KTAVFAFAALLTQPFFFASAEFANLDMLVASMIALTIMMAAEFVWALEKGLFAKRYLV
                     ATYLFVVAGVLAKGLIGIVLPGAVILCWLLATGRLSMLKSFFWWPAILLFLLLVSPWF
                     LFMESQFKGFLSYFFIHHHFERFTGTDFNNQEPFWFYLPALIVLTLPSSLWLLRFRIR
                     EFQQWPAHYKDISLLMVCWIGFIVLFFSMPASKPLGYIMPVLPAIATLAAIFMMPAWQ
                     DEVLKQRMLWATFIVSGIACVVAIGTVIQHAQKVATYQMAVQVKQIPGKDDQLVMLNK
                     YRFDLPFYLHLEHPAWIVTNWQEAANTPLADSWEKELLEAKDFDVQTANRNLLSYQAL
                     ARQVCHLHAHQAVWVAGDAGSATQFSALANDPPLVNVRGHTLWRLGPAEIARFCSTTT
                     AKVD"
BASE COUNT         2309 a         2353 c         2285 g         1901 t
ORIGIN      
        1 agccatgaca ttgttgccaa aggcctgcaa ctgctggaca acctgacgca ccgtggtgcc
       61 actggttacg accctaaact gggtgacggc gctggcctgc tgatgcaaat gccagacact
      121 ttcatacgcg atgaagcggc caagctaggc atgaccctgc ccgcagcggg ccaatatgca
      181 gtaggccaag ctttcttgcc acaaaacgca gacaaccgcg caaaatgcga agccattttt
      241 gcgcaaatca tcgccgaaga aaatcaaacc ctgctgggct ggcgcgatgt gccagtcgat
      301 aacagcaata tcgccgatgc agcacgtgcc gtagagccag tgatgcgcca ggtatttatt
      361 gccagcacag ccgctgacag caccactttt gaacgcaaat gttttgtcat tcgcaaacgt
      421 attgaacatg caattcgcgc actcaacctg caagacaagg cacagttcta catgccttca
      481 ttctcttccc gtacaattgt ctacaaaggc atgttgctgg cgaacgaagt tggcgtttac
      541 tataaagacc tgaaagacga acgtgtcgta tcagctttgg cgctggtaca ccaacgcttc
      601 tcaaccaaca ctttcccagc ctgggatttg gcgcacccat tccgcatgat tgcgcacaac
      661 ggggaaatca ataccgtgca gggcaacgtc aactggatgg cagcccgcca cgaaaccatg
      721 aagtctgccg tattgggcga agacctggaa aaactttggc cgctcattgc cgaaggccag
      781 tctgactctg cctgttttga caactgtctg gagttgttag tcgctggcgg ctatagcctg
      841 ccgcacgcca tgatgatgct gatcccggaa gcctgggctg gcaacccatt gatggatgaa
      901 gaccgccgcg ccttctacga ataccacgct gcattgatgg agccatggga tggcccggct
      961 gccgttgcat ttactgacgg caaaatgatt ggcgccaccc tggatcgcaa cggtttgcgt
     1021 ccagcacgtt acctggtcac agaagacgac atcgttatga tggcgtctga aatgggcgta
     1081 atcactttcc cacaagagaa aatcgttaaa aaatggcgtt tgcagccagg caaaatgttg
     1141 ctgatcgata tggagcaagg ccgcatcatt gatgacgaag aagtcaaaac tgaattggcg
     1201 caagccaaac cttaccgtca gtggatcgaa caaacccgtt tccacctgac cgaactgcca
     1261 agcgttggta gcggcgaagt caacctgaat gctgacttgc tggatacaca acaggccttc
     1321 ggctactcac aggaagacct gaaatttgtg ctgcagccta tgctggataa cggcgaagaa
     1381 ggctctggct ccatgggtaa cgatgcggca ctgccagtgt tgtccaacaa agccaaaacc
     1441 ttgtacaact acttcaagca gttgtttgcg caggtgacta acccgccaat tgacccaatt
     1501 cgtgaagagc tggtgatgtc cctggtgacc tttattggcc ccaaacctaa cctgctcgca
     1561 atcgacgaaa ccaatccacc attgcgcctg gaaagcagcc aaccggtatt gagcctggat
     1621 ggcctggaaa aactcaaaca gattgaatca ctgaccaaag gccagttcaa gtccatcgta
     1681 ctggacctga cttatgaggc agcccttggt aaagcaggca tgaagcaggc actggacgcc
     1741 ttgcatgaca aggccgaaaa agcagtgcaa gacggttaca acgtgttaat cctgtctgac
     1801 cgcaacgttt ctgccagtcg cattgccatt ccagcgttac tggcatgttc cgcaacgcat
     1861 gagtttttgg tcaaggctgg tttgcgtacc agcactggct tggtgattga caccggttca
     1921 gcacgtgaag tgcatcactt tgcactgctg gcaggttacg gcgcagaagc ggtatgccca
     1981 tggttggtat atgaaaccat gaagaccatg accagcgatg tggaaaaagc gggcaagaat
     2041 ttcgtaaaag ccatcggcaa aggtttgtac aaagtgatgt ccaaaatggg catctcgacc
     2101 taccagtctt actgtggcgc acaaattttt gaagcgattg gtttgaacag ccagttcgtc
     2161 aatgaatact ttaccggcac cgtcagccag attgaaggga tcggtctgga gcaagtctcc
     2221 gaagaaaccg tacgcttgca tagccaggca ttcggcaccg acccggtatt gtctaatgca
     2281 ctggaagccg gtggcgaata cgccttccgc gtacgtggtg aagagcactt gtggacacca
     2341 cagtccatca gcaaactgca gaacgccacc cgcaccggcc agtacgaaac ctataaggaa
     2401 tatgctgcgc tgatcaacga ccagacccgc cgtcacatga ctttgcgcgg cctgtttgaa
     2461 atcaaacctg ccggcaaagc catcccgctg gaccaggttg agtctgccaa agaaatcgtc
     2521 aaacgctttg cgactggcgc gatgtcactg ggctcgatct caaccgaagc acatgccaca
     2581 ctggcaattg cgatgaaccg tatcggtggt aaatctaaca ccggcgaagg tggtgaagac
     2641 gttaaacgct ttattccagt acaagtggat tccagcattg ccaaagtgct aggtagcgac
     2701 ctgatcgaag ccgatgtggc cttgaaagca ggcgactcca tgcgctcacg catcaagcaa
     2761 gtcgcttctg gccgttttgg cgtgactgct gaatacctga gcagtgcaga ccaaatccag
     2821 atcaagatgg cacaaggggc caaacccggt gaaggtggcc aattgcctgg tcataaagtt
     2881 tcgccatata ttgcgaaact gcgtttctcc gtgcctggcg taggcttgat ttcgccaccg
     2941 ccacaccacg atatttattc gattgaagat ctggcgcagt tgattcacga cctgaaaaac
     3001 gccaacccta aagcgtccat ttcagtgaag ctggtatcag aaaccggtat cggtactgtg
     3061 gctgctggtg tggccaaagc caaatctgac cacattgtgg ttgctggcca tgacggcggc
     3121 actggcgcct cacctatttc atccatcaaa catgctggta cgccatggga actcggcctg
     3181 gccgaaaccc agcaaaccct ggtattgaac cagttgcgtg gccgcgtggt attacaagtc
     3241 gatggccaga tgaaaactgg ccgtgacgtg ctgatcggtg ccttgctggg tgcggatgag
     3301 tttggctttg ccactgcgcc actggtcgtt gaaggctgta tcatgatgcg taagtgtcac
     3361 ctgaacacct gcccggtggg tgtcgcgacc caggatccag aactgcgtaa gaaatttact
     3421 ggccagccag agcatgtcgt caactacttc ttcttcgttg ctgaagaggt gcgtgagttg
     3481 atggccagca tcggtatcgc caaatttgat gacctgattg gccgcgctga tttgctagac
     3541 atgaaagccg gtatcgaaca ctggaaaatc aatggcctgg acttcagcaa ggtgttccac
     3601 ctgccagaaa tgccagccag cgtgtcacgc aaaaacaatg atgtacaaga ccacggcctg
     3661 gttaaagccc tggacaatca actgattgaa ctggcaaaac cagcgttgga aaaaggcgag
     3721 aaagtcagca tcgcgctgcc tatcgtcaac accaaccgta ctgtaggtac catgttgtca
     3781 cacgaagtgg ccagccgtta tggccatgca ggtttgcctg acgacaccat cagcgtcaaa
     3841 ttcaccggta cctccggcca gagttttgcg gctttcctgg cacagggcat tacgtttgag
     3901 ctgacaggtg aaggcaacga ctacgttggc aaaggcttgt gtggtggccg tatcatcatc
     3961 aaaccaccgg cgacattccg tggcttgtct tacgagaaca tcattgttgg taacaccgtg
     4021 atgtacggtg caactactgg ccaaagcttc ttcagcggcg tcgctggtga gcgcttctgc
     4081 gtgcgtaact ccggtgccac cgctgtcgtt gaaggcgtag ggaaccacgg ctgtgaatat
     4141 atgaccggcg gcacagtact ggttctcggt gcaacaggcc agaactttgc agcgggcatg
     4201 agcggcggca ttgcctatgt ctacgacatc gacggcaaat tcgcccagcg ttgtaacatg
     4261 agcatggtat ctctggacaa agtggaagcc gccgaagctg atgttggcaa agtacagcat
     4321 ctgggtcagc ctgacgaagt tattctgaaa ggcctgatcg agcagcacct ggcactgact
     4381 ggcagtccac gtgcaaaaga acttctggct gactggccag cttcacgtgc gaaattcgtc
     4441 aaagtattcc cgaacgaata caaacgcgca ctgacagaaa tggctgccgc ttcggccaaa
     4501 caagccgctt aatcgttttg taaattatag ccccgcctga gtgcggggcc ttgttaaggt
     4561 tgcgtctgta caaagcaaca gggaagagat gatttcacat gggtaaagtc acaggtttta
     4621 tggaatttga ccgtctggca gaagaaaact tgccagtcaa agatcgcgtc aaaaactaca
     4681 aagagttcgt cctgcatctg acagacgacc aggcaaaaca gcaaggtgca cgctgcatgg
     4741 attgcggcat tccgttctgt accacaggct gtccaatcaa caacatcatt cctgactgga
     4801 atgacttggt gtatcaccag gactggcgca gtgcgattga tgtattgcac tccaccaata
     4861 acttccctga tttcacgggc cgcatttgcc cggccccatg tgaagcggct tgcacattaa
     4921 atatcaataa cgatgctgtt ggcattaaat ccattgagca cgccattatc gacaaagcct
     4981 gggaaaacgg ctgggtcact ccgcaagtca accccaataa aaccggtaaa aaaattgccg
     5041 tggtaggttc aggccctgcc ggtctggctg ccgcacagca attggcacgt gcgggccaca
     5101 gcgtgaccgt gctggagaaa aatgaccgta tcggcggctt gctgcgttac ggcatccctg
     5161 acttcaaact ggacaaatcc aatattgacc gtcgcatcac acaaatggaa gccgaaggcg
     5221 tgaccttcaa ggtgaaccag aacgtaggtg agaacgtcag cgcagcggac ctcgagcgtg
     5281 acttcgacgc ggtgatcctg gccggtggtg ctgaattgcc acgtgacctg ccagtaccag
     5341 gccgtgaact ggatggcgta catttcgcca tggacttttt gacaccaaac aacaaaatgg
     5401 tggcgggcga caccgtgcct aaccagatca aggcgactgg caaacatgtt gtcgtcattg
     5461 gaggtggtga taccggctct gactgcgttg ggacttctaa ccgccatggc gcagccagcg
     5521 tcacccagtt cgagttgatg ccacaaccgc cagaaaaaga aaacaaatca ctggtatggc
     5581 caaactggcc actcaagatg cgcacctcca gctcacacga agaaggtgca gaccgcgact
     5641 ggtctatcac caccaaagag ctgatcggcg agaacggtaa actgaccgcg cttaaagggg
     5701 cacgtgtcga gtggaaggac ggaaaaatgg tcgaagtgcc tggctccgag ttcaccatca
     5761 aggccgacct ggtatttttg gccatgggct ttgtctcccc ggtacaaaaa gtgctggatg
     5821 cgtttggcgt ggaaaaagac aaccgtggta atgccaaagc cacgacagat ggcgaaggct
     5881 gctataaaac cagcaaagcc aaagtgtttg ccgccggcga tatccgccgc ggccaatccc
     5941 ttgtggtatg ggcgatccgc gaaggccgtc agtgtgcacg tgaggttgat gccttcctga
     6001 tgggtagctc caacttgcca cgctaattct gccgaatagc ttgtgaccat aaaacgccga
     6061 tgactttcat cggcgtttct tattgtgcta tcgcaaaaaa accgcacttt tgcagatgaa
     6121 acagcggctt ggcctttata atcatgcttt tacattttat tgacccatca tcaccgatgc
     6181 ggtcatcgcg acaaaccaaa tgactcaact gcagaacgat acctttttgc gcgccctgat
     6241 gcgccaaccc accgaataca cacccgtgtg gatgatgcgc caggcgggcc gttatttgcc
     6301 agaataccgc gagagccgta aaactgctgg cagctttatg cagctctgcc agagtccttc
     6361 atttgcaacg gaagtgacct tgcaaccctt ggaccgctac ccacaactgg atgcagccat
     6421 cctgttcagc gatatcctga ctgtgccgga tgccatgggt ctgggcttgt attttgaaga
     6481 gggtgaaggc ccgaaatttg agcgtacctt gcaagctgag gaggatatcc tcaagctgga
     6541 agtaccggac atgaccaagc tacagtatgt gtttgatgct gtcagcagca tccgtaaggc
     6601 actcaatggc cgtgtgcccc tcatcggctt ttccggtagc ccatggaccc tcgcgaccta
     6661 tatggtcgaa ggtaaaggcg gcaccgattt cctgaatact aagaaaatgg gctatgccag
     6721 accagaactg ctgcatcgca ttctggaaat cacgtcacaa accgtcattc aatacctgaa
     6781 tgcccaaatt gctgctggcg cgcaggccgt gatgattttt gactcgtggg gtggcgctct
     6841 gtcgcatgac gcctatctcg aattctcgct tcactatatg cagaaaatcg tcgccggcct
     6901 gaccaagacc gcggaaggta aaacagtgcc cagcattgtt tttaccaaag gcggcggttt
     6961 gtggctggag caacaagcgg ccattggtgc agatgcctta ggcctggact ggacggttga
     7021 ccttgcacaa gcccgccaac gcgtaggtga tcaagtggcc ttgcagggca atatggatcc
     7081 agccattttg ctgagttcac cagccgcgat tgaaaaagag gttgcccgca ttctcgccag
     7141 ctatggccat ggtcatggcc atgtcttcaa cctgggacac ggcatcacac agtggacgcc
     7201 tcccgagcat gcagcgtgca tgctggaaac cctgcgcaag ctcagcccgc agtatcacgg
     7261 ctgagccatc gcatcacacc caaggttttg agtcattaac gacccggctc aatccacctt
     7321 ggcggtggtc gtcgaacaaa aacgggcaat ctccgcagga cctaaccgcc acaaagtatg
     7381 tccacggaca ttgaccagtg gcggatcgtt tgccaacgct gaaaattggg tagcgctccc
     7441 tgcatccccg gccacccaga ctgcctgatg cgcatgtaaa tgacatacct gccgcgcaag
     7501 ggcctgatag gaaagtaagt tcctgtttgc tgtttgcaca tcgaaatctt tcgcttccag
     7561 caattctttt tcccagctat ctgccaatgg cgtattggct gcttcttgcc agttggtgac
     7621 tatccaggcg ggatgttcca gatgcaggta aaacggtaaa tcgaaacggt atttattgag
     7681 catgaccaat tggtcatctt tgcctgggat ctgcttcact tgcacggcca tttggtatgt
     7741 agccactttt tgtgcatgct ggatcaccgt tcctatcgca acgacgcaag caatcccaga
     7801 cacgataaac gtcgcccata gcatgcgttg ttttaagacc tcatcttgcc aagctggcat
     7861 catgaagata gcggcaagtg tagcgattgc tggcagcaca ggcatgatat agcccagcgg
     7921 tttagaagct ggcatggaaa agaacaacac aatgaagccg atccagcata ccatcagcaa
     7981 gctgatatct ttatagtgtg caggccattg ctgaaactca cgtatgcgga acctgagtag
     8041 ccacagtgaa gagggcaagg ttaacactat taatgctggc aaataaaacc agaacggttc
     8101 ctggttatta aaatccgtgc ctgtgaaacg ctcaaaatgg tggtgaataa agaaatagct
     8161 caagaagcct ttgaactgcg actccatgaa caaaaaccat ggactgacca gtaatagaaa
     8221 taacaggatt gcgggccacc aaaagaaaga cttcagcatt gataatcgcc cggtagccaa
     8281 tagccaacag agaatgactg ctccaggcaa aactataccg atcaagcctt tagccaatac
     8341 gccggcaacc acaaaaagat aggtcgcgac taagtaacgc ttcgcaaaaa gacctttctc
     8401 aagtgcccag acaaattcgg cggccatcat aatggtgagc gcaatcatac tggccaccaa
     8461 catatccaga ttggcaaatt cggcactggc aaaaaagaaa ggctgtgtca gcagcgcagc
     8521 aaaagcaaat acagctgtct ttctatcgac atggcgagac agaaaccaaa acagtcccaa
     8581 tgccatcatt aaagcggaaa gcaatggggc aaagcgactg cacagcaagg atgggccgca
     8641 ggccttgaaa cttgccgctg tcagccaata aaacaaaggc ggtttatgga aatagggcaa
     8701 accatccagg tgtggcacca accagtcccc gctggtcgcc atctcccagg caatgccgac
     8761 atagcgcccc tcatcaggta aggccaaagg cctgcacacg gcagcggccc ctagccagaa
     8821 tattgttatg cctaaccaga ccagattc
//
LOCUS       DATMSK010000120         7480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_291258,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000120 DATMSK010000000
VERSION     DATMSK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291258"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VNZ84_04090"
     CDS             complement(<1..572)
                     /locus_tag="VNZ84_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWU66167.1"
                     /translation="MMFRFLKILSVCTTLFFAHAALAEPAAKPVILVFGDSLSAAYGI
                     PKEKGWVNLVAQRIKDNQLPYEVANASVSGETTAGGLSRLPAALQQFKPSIVVIELGA
                     NDGLRGLPTDAMKNNLEKMIAACKQINARVVLLGMLIPPNYGPKYTNGFKQVFLDLSQ
                     TYKTPFVPFFLDGVSGHSDLVIEDGLHPNV"
     gene            575..1282
                     /locus_tag="VNZ84_04095"
     CDS             575..1282
                     /locus_tag="VNZ84_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU66168.1"
                     /translation="MQAPYAVEAVSLSKTVVSNDTTIPILTNLNLVVPKKERLAIIGS
                     SGSGKSTLLGLLAGLDIASSGKVLIDGSDIGPLDEDGRAAIRAKHMGFVFQSFQLLGS
                     LTAFENVMLPLQLAGRSDASAIAMSALESVGLAARSTHYPKQLSGGEQQRVAIARAFA
                     AKPSILFADEPTGNLDAATGESIIELLFDLNLKSGTTLVLVTHDMNLARRCQRVLNLQ
                     NGQLKEIDIADKKAGAH"
     gene            1282..3795
                     /locus_tag="VNZ84_04100"
     CDS             1282..3795
                     /locus_tag="VNZ84_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWU66169.1"
                     /translation="MLVLSIAWRQCRSLWSSGEIRVLLFALILAVTATTSVSFFTDRI
                     QSGMQTQGNVLLGGDIVVMSDHAIPASYLAEAQKRQFQTTQTMEFPSMVLNGESSQLA
                     EIKAVESGFPLRGHLTISQSASRKQLATDIPAKGTVWIEPSLAKKLALNIGDSISIGD
                     RQLKVSALLLLEPSRGGDMFSIAPRVFMNSADVAATGLIQFGSRVKYQLLVANQANEQ
                     DVMQAYADWLKPQLGRGERVEDVRSARPEMRNALDKAQQFLGIASTVGVILAMVAMLL
                     ASIPYVQKSLDTYALMRCFGATKPLITQILLYQTLLLALVGSVIGCVLGFAAQYGLAI
                     FAERLFLETLPSPGWMPVILGFVTGFATLLTVVWPQLMRLQAVPALRILRQDLEGNVR
                     TNGLVFLPAFLVLAGLVFWHSGDIKLAGIVLAALVALVVVASVLALAGMRLIRALSKQ
                     EVGASQRFGVIQLGLMGFRRRPALTVAQVVGLSMGLMAILLLAFVRNDLLKNWQTSLP
                     ADAPNRFVINIQAEQIPGINALLTEQGIRHADIFPMVRARLEAVNGKLISAADYSDDR
                     AKRMAEREFNLSWAAEMQADNTSVAGQWWQVEDAGKHLLSLEQELAKTLKLNLGDQLT
                     YDVAGTKLNLTISNLRKVEWSSMHVNFFAVTPPKVLDNYAASYLTSFHLPADQEETIN
                     RLVKQFPNLTIINVSALMEQIQTLISKMSYAIEFVFGFSLLAGLAVLYAALMATREER
                     VRESTLMRVLGASRRQVISAMLVEFVSVGLLASVVAILFTNIAAYYLATLVLKVDYQF
                     NAWLTLEAVIAAIVLVPLAAWLVARHQFNQPPRALLQSI"
     gene            3834..5042
                     /gene="hemW"
                     /locus_tag="VNZ84_04105"
     CDS             3834..5042
                     /gene="hemW"
                     /locus_tag="VNZ84_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWU66170.1"
                     /translation="MIPIYPANSLSDAPLQGDVTLSGLTNPPPLSLYIHIPWCVRKCP
                     YCDFNSHEARQEIPEAAYVDALIADLEQATPLVWGRKIRSVFFGGGTPSIFSAEAIDK
                     ILSHVRMLTPLEYGAEVTLEANPGTVDIANFKGYRDAGVNRVSLGIQSFQPRYLKSLG
                     RIHDREQALAAAELALNTFDKVNLDVMYALPEQSLEDALKDAETACQIKPAHLSFYHL
                     TLEPNTPFHRTPPSLPDDDTSATMQEEIEKILAANGYQHYETSAFAQPGQQCQHNLNY
                     WTFGDYLGIGAGAHSKLSFHDKIIRQSRHKHPKRYLEAIQQGQVVDSEWQIGREDLAF
                     EFMMNALRLVDGVEAGLFQQRTGLPLRTIQSTLQAAQQQGLLAEQSGVIAPTLKGQRF
                     LNELLGMFLP"
     gene            complement(5651..6334)
                     /locus_tag="VNZ84_04110"
     CDS             complement(5651..6334)
                     /locus_tag="VNZ84_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66171.1"
                     /translation="MMVGLILAVSLWAGMHQPAKAALGIANPKHEQAMLWPLLPGESV
                     NSLAAKLYPQSPVLQQRFVQQSITLSRSRGILLRPDERSETAQLIAVPNAQTLHTLTH
                     RIKKADEVDTQAFVTPGLVMSVQLTQDTQPSSGWHLPKLTLPSVHLPTALSFKDYSFS
                     VTNLTRRFAAIKTHLLETYEANAQAIARTPVKRLYQTPQLAVIVVSALLLLVLSVVWG
                     ILEERQPRS"
     gene            complement(6382..>7480)
                     /gene="argH"
                     /locus_tag="VNZ84_04115"
     CDS             complement(6382..>7480)
                     /gene="argH"
                     /locus_tag="VNZ84_04115"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWU66172.1"
                     /translation="EKIGDAGKRLHTGRSRNDQVATDVRLWLRSVVDDTIAGIRALQT
                     SLLDLAEKNAATIMPGFTHLQVAQPVTFGHHLMAYFEMLNRDAERFADARKRINRLPL
                     GAAALAGTSYPIKRELVAELLGFYSVCQNSLDAVSDRDFAIEFNAAASLLMMHLSRFS
                     EELILWMSPRFGFIDIADRFCTGSSIMPQKKNPDVPELVRGKTGRVYGHLIGLLTLMK
                     GQPLAYNKDNQEDKEPLFDVADTLLVTLQIYADMLRGITVKADNMRQAALEGFATATD
                     LADYLVKKGMPFRDAHEVVAHAVKAGVESGRDLAEFSLAELQAFSATIAEDVFEVLTL
                     EGSVASRNHTGGTAPEQVRRAIAQARQQLGS"
BASE COUNT         1712 a         1813 c         2078 g         1877 t
ORIGIN      
        1 atcacatttg ggtgcagccc atcttcaatc accaaatcac tgtgcccact cacaccatcc
       61 agaaaaaatg gcacaaatgg tgttttgtag gtttgcgata aatccagaaa cacctgttta
      121 aaaccattag tgtatttggg gccatagttc ggtggaatga gcattcctaa caaaacgaca
      181 cgcgcattaa tctgcttgca ggcggcgatc attttttcga ggttattctt catcgcatct
      241 gttggtaagc cgcgcaggcc atcatttgcg cctagttcta tcaccacaat actgggttta
      301 aactgctgaa gtgcggcagg caaacggctg agtccgccag cggttgtttc accactgaca
      361 ctggcgttgg caacttcgta gggtaattgg ttgtctttta ttcgttgtgc gaccagattg
      421 acccagcctt tttctttggg aatgccatag gcggcggata aactgtctcc aaacaccaga
      481 ataaccggtt tggctgcagg ctcagccagg gcggcatggg caaaaaatag agtagtgcaa
      541 acgcttaata ttttgagaaa acggaacatc attaatgcaa gccccttatg ctgttgaagc
      601 agtgtcttta agtaaaacgg ttgtttccaa tgatacaaca atccccattt tgactaattt
      661 gaatctggta gttcctaaaa aggagcgcct tgcgatcatt gggagttcag gttctggtaa
      721 atctaccttg ctgggtttgc tggcggggct ggatattgcc agcagtggca aggtgttgat
      781 tgatggcagt gatattggcc cgttggatga agatggccgc gccgccattc gcgccaagca
      841 tatgggcttt gtctttcaat cgtttcaatt attgggctca ctgactgcgt ttgaaaatgt
      901 gatgttgccg ttgcagttgg cgggtcgcag tgatgcatct gctatcgcga tgtctgcact
      961 ggaaagtgtc ggccttgcgg cacgctcaac gcattatcca aaacaattat ctggtggtga
     1021 acaacaacgg gtggcgattg ccagggcgtt tgcagccaag ccttcgattt tatttgccga
     1081 tgagccaact ggtaatctgg atgcggcgac tggcgaatct atcattgagt tgctgtttga
     1141 cctgaatttg aaaagcggca cgactttggt gctggtgacg catgatatga atctggcgcg
     1201 gcgttgccag cgtgtgctga atttgcagaa tggtcaactc aaagaaattg atattgcaga
     1261 caaaaaagca ggggcgcatt aatgctggtg ctctctattg cctggcgcca gtgtagaagc
     1321 ctgtggtcgt caggtgaaat ccgtgtcttg ctgtttgcgt taattctagc ggtgacggca
     1381 actacttcag tcagtttttt cactgaccgc atccaatccg gtatgcaaac ccagggcaac
     1441 gtgctgcttg gcggcgatat agtggtgatg tcagaccatg ccatcccggc aagctatttg
     1501 gcagaagcac aaaagcggca attccagact acgcagacca tggagtttcc aagcatggta
     1561 ctgaatggcg agagcagcca gctcgccgaa atcaaggcgg tggaatctgg ctttccatta
     1621 cgcggtcatt tgacgattag tcaatcggcc tcacgcaaac aactggcgac agacattccc
     1681 gccaaaggca cggtctggat agagcctagc ctggcaaaaa aactcgcatt gaatattggc
     1741 gatagcatca gcataggcga tagacagctc aaggtttcgg ccttattact gctggagcct
     1801 tcgcgtggcg gtgatatgtt cagtattgcg ccacgggtgt ttatgaatag cgccgatgtc
     1861 gcggcgactg ggttaatcca gtttggtagc cgggtgaaat atcagctact ggttgccaac
     1921 caagcgaacg aacaagacgt aatgcaggct tatgctgact ggctgaaacc acaattaggc
     1981 agaggcgagc gtgtagaaga tgtgcgtagt gcacggcccg aaatgcgcaa tgcgctggat
     2041 aaagcgcaac aattcctcgg cattgcttcc accgttggcg tgattctggc catggtcgcc
     2101 atgttgctgg cgagcatacc ctatgtgcaa aaaagcctgg atacttatgc gctgatgcgt
     2161 tgctttggcg caaccaaacc actgattacg cagattctgc tctaccagac gctgcttctg
     2221 gcgttggttg gtagtgtcat tggttgtgta ctgggctttg ccgcgcaata tggactagcc
     2281 atcttcgctg agcgcctgtt tttggagact ttgcctagtc caggctggat gcctgtcatc
     2341 ctgggttttg tcaccgggtt tgccacttta ctcaccgtcg tctggccgca attaatgcgc
     2401 ttgcaagcgg tgccggcatt acgcattttg cggcaggatc tggaaggcaa tgtgcgcacc
     2461 aacggcctcg tatttttacc cgcatttctg gtactggccg gcctggtgtt ctggcattct
     2521 ggggatatca aactggcggg cattgtattg gctgcgctgg tggctttggt cgtggtggcc
     2581 agtgtgctgg ccttggccgg tatgcggctg atacgcgctt tatccaaaca agaggtgggg
     2641 gcttcgcaac gctttggtgt gattcaactg ggtttgatgg gcttccgtcg ccgtccggcc
     2701 ttgaccgtgg cgcaagtggt cggtttgagc atgggcttga tggcgatttt attgctggcc
     2761 tttgtacgta atgatttgct gaaaaactgg caaacctcgt taccagcaga tgcgcctaac
     2821 cgctttgtga ttaatatcca ggccgagcag attccaggga ttaatgcgtt actgactgag
     2881 caaggcattc gccatgcgga tattttcccc atggtgcgcg cacgcctgga agcggtgaat
     2941 ggcaaactga ttagtgcagc agattactct gatgatcgcg caaagcgcat ggcagaacgg
     3001 gaattcaatt tgtcatgggc ggctgagatg caggcggata atacatctgt ggccgggcag
     3061 tggtggcaag ttgaagacgc aggtaaacat ctgttgtctt tggagcagga actggcaaag
     3121 acgctaaaac tgaatttggg cgatcaactc acttatgacg tggctggcac caagctcaac
     3181 ctgaccattt ccaacctgcg taaggtagag tggagcagca tgcatgtaaa tttcttcgcc
     3241 gtgacgccgc ccaaagtgct ggataactac gcggccagtt atctcaccag cttccacctg
     3301 cctgcagatc aggaagaaac cattaatcgc ctggtcaaac agtttcctaa cctgaccatt
     3361 atcaatgtgt ctgcgctgat ggagcagatt caaaccctga ttagcaaaat gagttatgcc
     3421 atcgagtttg tatttggttt tagcttgttg gcgggattgg ccgtacttta tgcagccctg
     3481 atggcgacgc gtgaggaacg cgtacgcgaa agcacgttga tgcgagtact gggggcttca
     3541 aggcggcaag tgatctctgc catgctggtc gagtttgtca gtgtcggttt gctcgccagt
     3601 gtggttgcga ttctgtttac caatattgcg gcgtattacc tggcgaccct ggtgctcaag
     3661 gtggattacc agtttaatgc ctggctcact ttggaagccg tgatcgcggc gattgtgctg
     3721 gtgccattgg ccgcctggtt ggtagcgcga caccagttta atcaaccgcc cagggcattg
     3781 ttgcaaagca tataggtcag taagctgtga gtgttccgag ttaaaatgcg cgcatgatcc
     3841 ccatttaccc tgccaacagt ttaagtgacg cgcccctgca gggcgacgtc acgctgagcg
     3901 gcctgactaa tccgccacca ctgtcgctct atatccatat tccgtggtgc gtgcgcaaat
     3961 gcccgtattg cgactttaac tcgcatgaag cgcggcaaga gattcccgaa gccgcttatg
     4021 tcgatgcctt gattgctgac ctggaacaag ccacgccgct ggtatggggg cgcaaaatac
     4081 gcagtgtgtt ttttggcggg ggtacgccca gcattttttc cgccgaagcg attgataaga
     4141 tactgagcca tgtgcgcatg ctgacaccgc tggaatatgg cgcggaggtc accttggaag
     4201 ccaaccccgg cacggtagat atcgccaact tcaaaggtta ccgtgatgca ggcgtgaacc
     4261 gggtgtcgct gggtatccag agctttcagc cacgatatct caaatcccta ggccgtattc
     4321 atgatcgtga acaggcgctc gcggcggcag agttggcatt gaacaccttt gataaggtga
     4381 atctggacgt gatgtatgcc ttgccagagc aaagcctgga agatgccttg aaagacgccg
     4441 aaaccgcctg ccagatcaag ccagcacatt tgtcgtttta tcatctgacg ctggagccga
     4501 acacaccgtt tcaccgcacg ccacccagct tgccggatga cgataccagc gccaccatgc
     4561 aggaagaaat cgagaagatt ttggccgcga atggttacca gcactacgaa acgtcggcgt
     4621 ttgcccagcc gggtcagcaa tgtcagcaca acctcaacta ttggaccttt ggcgactatt
     4681 taggcatagg cgccggtgcg cattctaagt tgagttttca cgacaagatc attcgccaga
     4741 gccggcataa gcatccgaaa agatatctgg aagccattca gcaagggcag gtggtcgata
     4801 gtgaatggca gattggccgt gaggacctgg cgtttgaatt tatgatgaat gcgttgcggt
     4861 tagtggatgg cgttgaggcg gggttgtttc agcaacggac gggattacca ttaaggacta
     4921 ttcagtcgac cttgcaggcg gcgcagcaac aggggctgtt ggcagagcag agtggggtga
     4981 ttgcaccgac attgaagggg cagcggtttt tgaatgagtt attggggatg tttttgccgt
     5041 aactcttagt ggtagtaaga gtttgaaaga gtgtttttcg ctttttcagc gagttacttt
     5101 tctttgcgtg cccaaagaac aagtaaccaa aagaaagtgc accccagctt ccgcttattt
     5161 cctgcgttgc tcaccaaaat aagcgctcgc agaactcatc ctagcggcct gcacaagacg
     5221 cagtcgaccg cgggattcgg acagccgctc gcttcatctt attttgcctg tgcgactcgg
     5281 cgagtcagat ggggacccga acacctactg tgcgccatcg atttgaggat ttagcaaagg
     5341 actttgattt ttgaaccgtc caaaattccg caatgtgagt ttggcgagaa ccatcccatt
     5401 ccatcacgct gagtagcgga aacaaaataa gagtagaggg agcgactgtc cgagctccgc
     5461 aacaagccac gaattgtgtg gcttgtcagg gcgagtttcg tgaccgactt attttgttga
     5521 gcaacgcaag ggagccgaag gccgggatgg ctgggtgccg tcttctttgg ttactttctg
     5581 tggggcaagc aacagaaagt aactcgccat caaggcgaaa aaagaatttt gaggcgtgat
     5641 aaaccaatcc ctatgaccta ggctgccgct cttccaaaat cccccaaacc acacttaata
     5701 ccagcaacaa taacgcactc accacaatca ccgccaattg cggtgtttga taaagccgct
     5761 tcacaggtgt gcgcgcaatt gcttgtgcat tcgcctcata agtctctaac aggtgtgtct
     5821 tgattgccgc gaaacgccta gttaggtttg tgacagaaaa agaataatcc ttgaacgaaa
     5881 gcgcggtagg taagtgcact gagggtagcg tgagctttgg caaatgccag cccgagcttg
     5941 gctgggtgtc ttgtgtgagt tgcacactca tcaccaaccc cggtgtgacg aacgcctgcg
     6001 tatcgacttc atcggctttt ttaatgcggt gcgtgagggt gtgcaaggtt tgtgcgttgg
     6061 gcacggcgat gagttgcgcg gtttcactgc gttcatccgg gcgcagcaga atgccgcgac
     6121 tacggctgag cgtgatggat tgctgcacaa agcgttgttg caataccggg ctttgcggat
     6181 agagtttggc ggccaggctg ttgacgcttt cgccaggcaa tagcggccat aacatggctt
     6241 gctcgtgttt agggtttgcg atgcctaacg ctgcttttgc cggctggtgc atgccagccc
     6301 acaagctgac ggctaatatg agccccacca tcatgaccgg gtatgatttg cgcttactcg
     6361 gtagatggta gctgtgggtc atcagctgcc taattgttgc ctggcttgtg caatggcgcg
     6421 gcgcacttgt tctggtgcgg tgccgccggt atggttgcgg ctggcgacgg agccttccag
     6481 tgtcagcact tcaaatacat cttccgcaat cgttgcacta aatgcctgta actctgccag
     6541 gctgaattca gccaggtcac ggccagactc cacgcctgct ttgacggcat gggcgaccac
     6601 ttcatgcgca tcacggaatg gcatgccttt tttcaccagg taatcggcga gatcagtcgc
     6661 ggtggcaaaa ccttccagtg cggcctggcg catattgtca gctttgacgg tgatgccgcg
     6721 cagcatgtca gcgtaaatct gcaaggtcac cagcaaggta tcagccacat caaacagagg
     6781 ttctttgtct tcctgattgt ctttgttgta agccagtggc tggcctttca tcagggtcag
     6841 cagacctatc aaatgcccat acacacggcc ggttttaccg cgcaccagtt ctggcacgtc
     6901 agggtttttc ttttgcggca tgatggacga accggtgcag aagcgatcgg caatatcaat
     6961 aaagccgaag cgtgggctca tccataaaat caactcttca gagaagcgtg acaggtgcat
     7021 catcagcaat gaggcagcgg cattaaattc gatggcgaag tcgcggtcag atacggcgtc
     7081 cagtgagttc tggcacacgc tgtagaaacc tagtaattca gccaccagtt cgcgtttgat
     7141 tgggtagctg gtaccggcca atgctgcagc gcccaaaggc aagcggttga tacgtttacg
     7201 cgcatcggca aagcgctctg catcgcggtt gagcatttca aagtaggcca tcaggtgatg
     7261 accaaaggtc actggttgag ccacttgtaa atgcgtaaag cccggcatga tagtggccgc
     7321 gtttttttca gctaaatcca gcagtgaagt ttgcagggcg cgaatacctg caatggtgtc
     7381 gtcaaccacg ctacgtaacc ataagcgcac atctgtggct acctggtcat tacgtgatct
     7441 gcctgtgtgt aagcgtttac cggcatcgcc gattttttct
//
LOCUS       DATMSK010000121         5633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_314992,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000121 DATMSK010000000
VERSION     DATMSK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314992"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1810)
                     /gene="msbA"
                     /locus_tag="VNZ84_04120"
     CDS             complement(77..1810)
                     /gene="msbA"
                     /locus_tag="VNZ84_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HWU66173.1"
                     /translation="MSDTQSTWTQLSRLMAYIKPYWLIFAAALICLVILSASNTGFLS
                     TIKHVTDEGFAGHAETSARVTLAAMLCGLLFVRALAGFGSNYLIRIVGRRVIEDVRNH
                     VFGHMLSLPASFFDQHAIANITTKMTFDCEQLYSAVTKVIISSIRDSLTILGIVLYML
                     YLDWKLTLVFLMLAPIMTRYLKKMTPKLRNSGKQVQSSMSEMTQIIEEAASGQRVVKI
                     FNGEAYEYERFSSVVGKNRQMMVRLGRISGLNSMFVEMLAAIALGMVIYYSAGQFTAG
                     EFAAFIGALLMLIGPVKAIASVNEDVQIAVTACQSVFAVLDAPAEMNLGTLVPKATIK
                     EIQFQNVDFQYPYSKHAALKNLNLTIKAGESIALVGMSGGGKSTLVNLLPRFHEIRHG
                     SITIDGVDIQQLALQALRQQFALVSQDTVLFNDTIYHNIAYGSLRGVSKEDVIAAAKA
                     AYAWEFIEKLPQQLDTHIGDRGLRLSGGQRQRLSIARAMLKNAPILILDEATSALDNE
                     SEHKVQAALNQLMLGKTTIMIAHRLTTIQHASRIIVMDQGQIVEEGTHEQLIQSGGKY
                     AALYNKYEISK"
     gene            complement(1813..2790)
                     /locus_tag="VNZ84_04125"
     CDS             complement(1813..2790)
                     /locus_tag="VNZ84_04125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWU66174.1"
                     /translation="MPKVSRILVITLSNIGDAVMTTPVLAALHQRYPQARIDLVCDVR
                     SSALFKPCPYLGQLFERSKSSGWQGYLQLIGKLRQARYDIIVDLRTDFLGYLLRGKQK
                     FKKRSNASSMHIHSVEKHMAAIRSLVGQALPLARLWVSEQDQYLVSAQYPSAGRLLAI
                     GPGANFSGKIWPVERYVAVANALTTYFDGVVLLGSQADQQYAQSFSVQCQLPVYDACG
                     RLTLPQSYALLKQCHFFLGNDSGLGHMAAAASLPTLTLFGPGSPLRYLPWSQLAYFIQ
                     DPNQDIGGITVQKVVNKLKTLLMQLEDQSNEPETSHASVAGDVDDGGYK"
     gene            complement(2801..3853)
                     /locus_tag="VNZ84_04130"
     CDS             complement(2801..3853)
                     /locus_tag="VNZ84_04130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU66175.1"
                     /translation="MPKVLWWGRSDLNYSRNRICRRLLQSLGYELIDFAPRISALADI
                     EVQLKGLVADLVWVPCFRHRDVAAATRWARKKNIPVVFDPLISAWQKQVYERRKYAAD
                     SLRAKWLLNREAKYLQAVDLVLLDTEPQRQMFIQRFKLASVKTARVYVGAEQPLFVPI
                     VAERSPALIEVLFYGSFIALHGVDIVIAAARMTAGQSHIQWVLLGEGPAKASAQLQAA
                     GLSNVRFESSVPYTQLPRRIAQADIVLGVFSDSEKAGQVIPNKVFQALACARPVITRA
                     SAAYPVEFSHEPEKGLWQIDAANPVALAEAVMQWSRCSRDKEAEAYDLYRTHFCEPQL
                     QQQLKCALGALGLQNA"
     gene            complement(3846..4958)
                     /locus_tag="VNZ84_04135"
     CDS             complement(3846..4958)
                     /locus_tag="VNZ84_04135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWU66176.1"
                     /translation="MTPKLVTYQDADCFAASLDLTIVNDLPRNPSIKRILIIKWGGMG
                     DIVISTAIMEDIRQAFPQAELHLNTMPAWQSLFAHDPRFSRIWCVNLQQRPGRWRAYR
                     QWLTEVVAMQYDLIIDLQTNDKSRSLLTMLRLMGKAPRLLVGNHPQFPYTVRQPQRLP
                     QAHSFQIMQQTLLSAGIPTSTNGPKLYISSSAVDSAELLLAQHGLAPKSFAVFLCGSH
                     AGGLTKRWGGAHYSALAEKCIQQGERVVLLGGRDEAEECSAIASQHPQQVVNLCGQTS
                     LLEVPVICGQAKYIVANDTGTAHLAAATKTPMTVICGPTNPLRVKPPGSQVIALQLDV
                     PCKNCYAKQCSHHSCMRQLSPSQLLSHLPVSGALDA"
     gene            complement(4948..>5633)
                     /locus_tag="VNZ84_04140"
     CDS             complement(4948..>5633)
                     /locus_tag="VNZ84_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWU66177.1"
                     /translation="LAWGLLLGAGVVIALSGARGYLYIYSLMLLLGLWRCQPGWKIWL
                     IVLSVPLLVAIFSSLLNPSLAKYKLANTQAITANNQSLFDRANHALSYRLNLWETGLH
                     MWQAEPLTGVGSNNYKRAYQQYSSRADDPFVANPTHTHHIYVEWLAETGLVGGFGLLA
                     IMVLCVRWFRQAAITNQHQAWPYALPLMVIYFPINTTQPMLVAWWFPVLMLLVCAFIA
                     SLEGNNHDA"
BASE COUNT         1504 a         1549 c         1301 g         1279 t
ORIGIN      
        1 gtccgaatcg actagtaaaa cttgtgttgc gtcgagaggt gagactatag caaacttaca
       61 aaaacagtgt caatagttat ttgctaattt cgtacttatt gtagagcgca gcatatttcc
      121 cgccagactg gataagttgc tcatgcgtcc cctcttccac gatttgacct tgatccatca
      181 caatgattcg gctggcatgc tgtatggttg tcaggcggtg agcaatcatg atcgtggttt
      241 tacctaacat aagttggttg agtgcagctt gtactttgtg ttcagactca ttatccaggg
      301 ccgaggttgc ctcatccaga atcaggatag gtgcattttt gagcattgcg cgtgcgatcg
      361 acaggcgctg acgctgcccc cctgacagac gcagcccgcg gtcaccaata tgtgtatcca
      421 gctgctgtgg cagtttctca ataaactccc aggcgtaagc tgccttggca gcagcaatca
      481 catcctcttt agaaactcct cgcaaactac cataagcgat attgtgatag atcgtgtcgt
      541 tgaacaacac ggtatcctgg cttaccagag caaattgctg cctcaacgcc tgtaatgcta
      601 gctgctggat atcaacgcca tcaatggtaa tgctgccatg ccggatctca tgaaagcgtg
      661 gcaacagatt gaccaaggtt gatttaccac ctcccgacat cccaaccaag gcaatacttt
      721 cgccggcctt gatagtcagg ttgagatttt ttaatgcggc atgcttgcta taaggatatt
      781 ggaagtcgac attctggaat tgaatttctt tgatggtcgc cttcggcacc agcgtaccca
      841 ggttcatttc tgccggcgca tccaataccg caaagacgct ctggcaggca gtcaccgcaa
      901 tctgcacatc ctcattcact gaggcaatcg ccttcactgg cccaatcaac atcagtaaag
      961 caccaataaa cgctgcgaac tcaccagcag taaactggcc tgccgaataa taaattacca
     1021 tccccaacgc aatcgctgcc aacatctcga caaacatgct attgagtccg gagatacgcc
     1081 ccagccggac catcatttga cgatttttgc caaccacact gctgaaccgc tcatattcgt
     1141 acgcttcgcc attaaaaatc ttaacgacac gctgcccact ggccgcctct tctatgatct
     1201 gcgtcatctc agacatgctg gactggacct gcttgccaga attgcgtaat tttggagtca
     1261 tctttttgag atagcgcgtc atgatgggag ccaacatcaa aaaaaccagt gtgagcttcc
     1321 agtccagata caacatatac agcactatgc cgagaatggt caggctgtcg cgaatagatg
     1381 aaataatgac tttagtgaca gcactatata gttgctcgca atcaaacgtc attttagtgg
     1441 tgatattggc aatggcatgc tgatcaaaga aactggcagg caagctcaac atatggccaa
     1501 aaacatggtt acgcacatct tcaatcacac gccgcccgac aatgcgtatc aagtaattgg
     1561 agccaaagcc agccagcgcc ctgacaaaca acaagccaca caacatggca gccaatgtaa
     1621 cgcgggcact ggtttcagca tgcccggcga acccctcgtc agtgacatgc ttaatcgtgc
     1681 tcaaaaaacc ggtattgctg gccgagagaa tcaccagaca gatcaatgcc gccgcgaaga
     1741 tcaaccaata gggtttaata taggccatca gcctggaaag ttgcgtccag gtgctttgtg
     1801 tatcactcat cattatttat agccgccatc atcgacatca cctgcaacgg acgcatggga
     1861 agtttcaggt tcatttgatt gatcctctaa ctgcatcaat agtgttttta atttattcac
     1921 caccttttgc actgtaattc ccccaatatc ctggttcgga tcctgaataa aataggccag
     1981 ttgtgaccat ggcagatagc gtagcggaga gccaggccca aacaaggtta acgtaggcaa
     2041 actggccgcg gcagccatat ggcccaggcc tgaatcgtta cccagaaaaa aatggcattg
     2101 tttcaataag gcataagact gcggcaatgt taagcgtcca caggcatcgt atacaggcaa
     2161 ttggcattga acgctaaatg actgcgcata ttgctgatca gcctgactac caagcaacac
     2221 caccccatca aagtaagtcg tgagcgcatt cgccacagcg acatatcgct ctaccggcca
     2281 aattttccca gaaaagttgg ccccaggccc aatagccaat aagcgcccgg cagatggata
     2341 ctgtgcagag accaagtatt gatcctgctc actcacccat aaccttgcca aaggaagcgc
     2401 ctgaccgaca agcgagcgaa tggccgccat atgtttttct accgaatgaa tatgcatact
     2461 actggcattg gaacgcttct taaatttctg cttgccccgc agcaaatagc caagaaagtc
     2521 tgtacgtaaa tcaacgatga tgtcgtaacg cgcctgcctt aacttgccaa tcagttgcag
     2581 atatccctgc cagccactgc ttttgctccg ctcaaagagc tgacctagat atgggcaagg
     2641 tttaaacaac gcagaagatc gcacatcgca aaccaggtca atacgcgctt gcggatagcg
     2701 ctgatgtaat gcggccagta ccggcgtagt catgacagca tcgccaatat tagacaaggt
     2761 aatcacaagg atacgtgaca ccttgggcat aacagcactc tcacgcattt tgtaaaccca
     2821 aagcacccaa tgcgcacttc aactgctgct gtaactgcgg ctcacaaaag tgagtccggt
     2881 agagatcgta cgcctcggcc tctttatcac gcgagcaccg tgaccactgc atcacagcct
     2941 cggccaaggc tacaggattg gccgcgtcaa tttgccatag tcccttctct ggttcatggc
     3001 tgaactccac cggataggcg gcactggcac gcgtgattac aggcctggca cacgccaacg
     3061 cttgaaacac tttattcggg atcacttgcc cggctttttc agaatcactg aacacaccca
     3121 acacaatatc cgcctgcgca atccgccgcg ggagctgcgt ataaggcacg ctcgattcaa
     3181 aacggacgtt agataaacca gccgcctgca actgcgcact cgctttggcg ggcccctctc
     3241 ccagcaacac ccactgaatg tgcgactgac ccgctgtcat cctggccgca gcaatcacaa
     3301 tatcgacgcc gtgcaaggca ataaagctac catagaacaa tacttctatt aacgcaggcg
     3361 aacgctccgc aacaatcggc acaaataaag gctgctcagc acccacatag acacgggccg
     3421 tcttaaccga tgccagttta aaccgctgga taaacatttg ccgttgcggc tctgtatcga
     3481 gcaagaccag atctacggct tgcagatatt tggcttcacg gtttaataac catttcgccc
     3541 gcaaactgtc ggctgcatat ttgcgccgct catatacttg tttttgccag gcgctgatta
     3601 atgggtcaaa tactacagga atattttttt tacgtgccca tcgcgtcgcg gcagccacat
     3661 cgcgatgccg gaaacaaggc acccagacca gatcggccac cagcccttta agctgtactt
     3721 caatatcggc cagcgcactg atacgcggag caaaatcgat caactcatag cccagcgact
     3781 gtaataaccg ccggcaaata cgatttctgg aatagttgag gtcagaccgc ccccaccaaa
     3841 gcactttagg catctagcgc ccctgaaacg ggcaaatgtg acagcaattg cgatggagat
     3901 aactggcgca tgcaggaatg atggctgcat tgcttggcat aacagttctt acacgggaca
     3961 tccagttgca gcgcaatgac ctgcgaacca ggcggcttga cgcgtaacgg gttagtcggg
     4021 ccgcaaatca cagtcattgg ggtcttggtc gctgcagcca gatgcgcagt gcctgtatca
     4081 tttgccacaa tatatttagc ttgcccgcat atcacaggca cctcgagcaa actggtttgc
     4141 ccgcacaaat tcaccacctg ttgcggatgc tggctggcaa tcgccgaaca ctcttcggcc
     4201 tcatcacgcc cacccaataa caccacgcgt tcgccttgct ggatacactt ttcagccagt
     4261 gcagaataat gtgcacctcc ccagcgcttg gtgaggccac ctgcatgact accgcacaga
     4321 aaaaccgcaa atgatttagg cgctaaccca tgttgcgcca ataacagctc ggcagagtct
     4381 actgccgatg atgagatata cagcttgggg ccattggtcg acgtcgggat gcctgcagat
     4441 agcaacgttt gctgcatgat ctgaaagctg tgtgcttgtg gcaaacgctg cggctgccta
     4501 accgtgtacg gaaactgcgg atgattgccg actaataacc ttggagcttt gcccatcaac
     4561 ctgagcatgg tcagtaaact gcgactcttg tcattagtct gcaaatcaat aatcaaatcg
     4621 tactgcatcg caactacttc agttagccac tgtcgatacg cacgccagcg ccctggcctt
     4681 tgttgcaggt tgacacacca gatacgacta aacctgggat catgcgcaaa tagtgactgc
     4741 caggctggca tggtattcag gtgtaattcc gcctgtggaa atgcctggcg aatatcttcc
     4801 atgattgccg tgctgatgac gatatcgccc atgccacccc atttgataat caggatacgc
     4861 ttgatagacg gattgcgcgg caagtcattc acaatggtga gatcaagact ggctgcaaag
     4921 cagtccgcat cctggtaggt caccagctta ggcgtcatga ttgttacctt ccagactggc
     4981 aatgaaggca cacaccagca acatgagtac tggaaaccac catgcaacca acatgggttg
     5041 ggtggtattg atcgggaaat aaataaccat caatggtagg gcatacggcc atgcctgatg
     5101 ctggttggtg atcgctgcct gcctgaacca gcggacgcac agcaccataa tcgccagcaa
     5161 cccaaagccg ccgaccaacc cggtctcagc cagccactcg acataaatat gatgtgtatg
     5221 ggttggattg gcgacaaaag ggtcatcggc gcgactggag tactgctgat aagcgcgctt
     5281 gtaattatta ctgccgacgc cagtgagagg ttcagcctgc cacatatgta ggccagtttc
     5341 ccacaagttc aggcggtaac tgagtgcatg attggctcta tcaaatagcg attggttatt
     5401 tgcggtaatc gcctgcgtat tggccagctt gtattttgcc aacgagggat ttaacagact
     5461 ggagaagatg gcgaccaata acggcaccga caacacaatc aaccagattt tccagccagg
     5521 ttggcaacgc cacaatccca gcaatagcat caagctgtaa atatagaggt agccgcgtgc
     5581 gcctgacaac gcaatcacga cccctgcgcc tagcagcaac ccccaggcca gcc
//
LOCUS       DATMSK010000122         6637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_244299,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000122 DATMSK010000000
VERSION     DATMSK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244299"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1621
                     /gene="purH"
                     /locus_tag="VNZ84_04145"
     CDS             17..1621
                     /gene="purH"
                     /locus_tag="VNZ84_04145"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWU66178.1"
                     /translation="MTIKRALISVSDKTGVLELAQSLHQLGVEILSTGGTAKLFRDNN
                     IPVKEVSDFTGFPEMLDGRVKTLHPKVHGGLLGRRDLPEHVAAMQQYGIENIDLLAVN
                     LYPFEATVAKPNCTLEDAIENIDIGGPAMVRSAAKNWNDVAVLTDAAQYGEVVSELKS
                     NNGVVSKATRFKLSVAAFNRIAQYDAAISNYLSAIDFAETESAGKPVLSQFPGQMNSN
                     FVKVQDLRYGENSHQQAAFYRDLYPAPGSLATAQQLQGKELSYNNIADADAAWEAVKS
                     FTGPACVIVKHANPCGVAVGVHALEAYSKAFKTDPTSAFGGIIAFNTTLDGAAAEAVS
                     KQFVEVLIAPDFTPEALAIFSAKANVRVLKIALPQGGNTAWEQGRNAVDSKRVGSGIL
                     LQSADNHEMQLADLKVVTKLHPTPAQMQDLLFAWNVAKYVKSNAIVFCGNGMTLGVGA
                     GQMSRVDSTKIAAIKAQNAGLSLQGSVVASDAFFPFRDGVDVLADAGASCVIHPGGSM
                     RDEEVIAAADERGLAMVVTGIRHFRH"
     gene            1724..2992
                     /gene="purD"
                     /locus_tag="VNZ84_04150"
     CDS             1724..2992
                     /gene="purD"
                     /locus_tag="VNZ84_04150"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWU66179.1"
                     /translation="MKILVIGSGGREHALAWKVSHNKEVSRVYVAPGNAGTATNPDMI
                     NVPLTKVEDLVKFAEDEQIALTIVGPEAPLSQGVVDAFRAADLKIFGPTKAAAQLESS
                     KDFAKAFMFRHDIPTAKYQTFSDVTQAHAYLDANGAPIVIKADGLAAGKGVVVAMTQE
                     EAHAAVDAMLSDNKLGDAGARVVIEEFLVGEEASFMVMVDGKNILPLATSQDHKRLLD
                     ADQGPNTGGMGAYSPAPVVTPDIHAKVMREIIVPTVEGMAKDGIPYTGFLYAGLMITP
                     AGDVKTLEFNCRMGDPETQPIMMRLKSDLVELAEHAVNGTLDQAKAEWDWRFALGVVM
                     ASANYPETPKLGDEITGLPKDVQDGHVFHAGTILKDGKVVTSGGRVLCVTALGETVKF
                     AQQQAYKILEQIQFDGAQYRKDIGYRAIKN"
     gene            3014..3928
                     /gene="hemF"
                     /locus_tag="VNZ84_04155"
     CDS             3014..3928
                     /gene="hemF"
                     /locus_tag="VNZ84_04155"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HWU66180.1"
                     /translation="MTEQIQPQAVFDYLQGLQARIVEAIELVDGKTFLQDSWQRPEGG
                     GGNSCLLEGGNVFERAGVGFSHVLGNKLPPAASVAHPEAAGRPWEAMGVSLVFHPHNP
                     YVPTVHMNVRFFVAKAQHAGEQDIWWFGGGMDLTPYYGFEDDCIHFHRVNKEALAPFG
                     ADYYRHFKKACDTYFYLKHRKEPRGIGGVFFDDFSELGFDKSFALQRAVGDAFINAYL
                     PIVQRRKDTAYGERERDFQAYRRGRYVEFNLIFDRGTIFGLQSNGRTESILLSMPPVV
                     KWRYDWKPEATSAEAKLYSDFLIEKDWV"
     gene            3931..5235
                     /locus_tag="VNZ84_04160"
     CDS             3931..5235
                     /locus_tag="VNZ84_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HWU66181.1"
                     /translation="MHVSVEARQQAQALLDFIDRSPSPWHAVETVADQLKAHGFKNLR
                     ENEPWQFKQGGKYFVVRDGGSIIAFVLGQAPLADDGFRIVGAHTDSPGLRLKPKAAYS
                     TEGLAQLGVEVYGGPILATFTDRDLSLAGRVVIQTAQGLESRLVRIDQAIARLPNLAI
                     HMNREVNEKGLVLNKQTGLPLIFGHAANTDSAKQLLHETLAGKLAIPASAIVSWDLAF
                     YDIQKGSFWGVEQEFIANSQLDNLASCHAALAALLSTETPQATSICALFDHEEVGSES
                     STGAGGSFLLDVIERICHSTGLTQEDKLRSFASSFFISADMAHAFHPNHAGSYEPCHH
                     VQVNQGPVIKTNANQRYSTNAVTAARFIQLCEKAGAPYQQYAHRTDLGCGSTIGPIMA
                     AQLGIATVDVGNPMWAMHSIRESAGVLDHNHMIATLQQHYAS"
     gene            5304..>6637
                     /locus_tag="VNZ84_04165"
     CDS             5304..>6637
                     /locus_tag="VNZ84_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWU66182.1"
                     /translation="MLKQLLRKKPIQPHAHTGLKKCLTAFDLALLGIGGAIGTGIFVL
                     TGIAAATQSGPAVVISFIIAAIAAAFAALSYAELSSSIGGSGSAYGYSYVAFGEFAAW
                     MMGWMLLLEYGVGAAAVANGWAGYFVNTLSNLGLHLPEHLTKAPVLGGSINLPAFAII
                     WILTLLLMVGVKESARANNIMVIIKLSTIAIFLALSVGHISTSNWQPFMPFGWFETLD
                     NGKNIGVLAGASLVFFAYFGFDAVSTAAEECQQPQRDLPIGLIASLTFCTIIYIIVSG
                     TLTGVIPYTELNTSSPVAYALTKLGYTWSSTLVATGVLAGLITVLLVLLYGLTRILFA
                     MSRDGLISPVFSTVNPDRQTPSKIILMCGFVVSIVAGFIPLGELAETVNIGTLASFIM
                     VCIGVIRLRQTHPDLPRPFKNPFNPLIPLGGVASCAALMSFLPAETWHRFLT"
BASE COUNT         1594 a         1781 c         1679 g         1583 t
ORIGIN      
        1 tccactcaca ttcattatga cgattaaacg cgcgcttatc agtgtttccg ataaaaccgg
       61 tgtactagaa ctggcacaat ccctgcacca gcttggtgta gaaattcttt ctactggcgg
      121 taccgccaaa ctgttccgtg acaacaacat tcctgtcaag gaagtcagcg actttaccgg
      181 atttcctgaa atgctggatg gccgcgtcaa aaccttgcac ccgaaagtgc atggtggttt
      241 gctgggtcgt cgcgacctgc cggaacacgt tgccgccatg cagcaatatg gcattgaaaa
      301 catcgactta ctggcggtca atctctatcc gtttgaggca accgtggcca agccaaactg
      361 cacgctggaa gacgcgattg aaaatatcga catcggcggt ccggccatgg tgcgctctgc
      421 tgctaaaaac tggaacgatg tcgccgtact cactgacgcc gcacaatatg gcgaagtcgt
      481 cagcgaatta aaatccaaca atggtgtggt ttcaaaagct acccgcttca aactgtctgt
      541 cgcggcattc aaccggattg cgcaatacga tgcggccatc agcaactact tgtccgccat
      601 cgactttgct gagaccgaat cagctggcaa accagtattg agccagttcc caggccagat
      661 gaacagcaac tttgtcaaag tacaagacct gcgctacggt gaaaatagcc accagcaagc
      721 ggcgttttac cgtgacctct accctgcccc tggcagcctg gcaacagcac aacaactaca
      781 aggtaaagaa ctcagctaca acaatatcgc tgatgccgat gccgcctggg aagccgtcaa
      841 atccttcacc ggcccggctt gcgtgattgt caaacatgcc aatccttgcg gtgtagctgt
      901 gggtgtacat gcattagaag cctatagtaa agcgttcaaa accgacccaa cctctgcttt
      961 tggcggcatt atcgctttca ataccacgct ggatggcgct gccgctgagg ctgtttccaa
     1021 acagtttgta gaagtattga tcgcccctga ctttacgcct gaagctctgg ccattttcag
     1081 tgccaaggcc aatgtgcgcg tactgaaaat tgctttacca caaggtggta acacggcctg
     1141 ggaacaaggc cgcaatgcgg ttgattccaa acgtgtcggt tccggcatct tgttgcaatc
     1201 tgccgataac cacgaaatgc aattggccga cctgaaagtc gtcacaaaat tacaccccac
     1261 accagcgcaa atgcaagacc tgctatttgc ctggaatgtg gcgaaatatg tgaaatccaa
     1321 tgccattgtt ttctgtggca acggcatgac attgggggta ggtgcaggcc agatgagccg
     1381 cgtcgacagc accaagattg ctgccatcaa ggcacaaaat gcgggcttga gcctgcaagg
     1441 ttctgtcgtt gcttctgatg ccttcttccc attccgcgac ggtgtagatg ttttggcaga
     1501 cgctggtgca agctgcgtca ttcatccagg tggtagcatg cgcgatgaag aagtgatcgc
     1561 tgctgccgat gagcgtggcc tggcgatggt cgtcacaggt atccgccact tcagacatta
     1621 attaaccttg ccatcgcggt cattaaggcg atgccacttc atgcagctat gatgtgagcc
     1681 ccttctgcac cacgatggcc ttaacattta ggcacttaat cttatgaaaa ttttagtgat
     1741 tggttcaggt ggccgcgagc atgcgctggc ctggaaggtt tcacataaca aggaagtcag
     1801 tcgtgtttat gtggcacctg gcaacgctgg tacagccacc aacccagaca tgatcaatgt
     1861 gcctctgacc aaagtcgaag acctggtcaa atttgcggaa gatgaacaaa ttgcactgac
     1921 catcgttggc ccggaagcgc ctttgtcaca aggcgtcgtc gacgcattcc gtgctgctga
     1981 cctgaaaatc tttggaccga ccaaagccgc ggcgcaactt gaaagctcca aggactttgc
     2041 caaagctttc atgttccgtc atgacatccc aaccgcaaaa taccagacgt ttagtgacgt
     2101 cacgcaagca catgcttatc tcgatgccaa cggcgcaccg attgtgatta aagccgatgg
     2161 cctggctgcc ggtaaaggcg ttgtcgttgc catgacacag gaagaagcac atgcggcagt
     2221 ggacgccatg ctatctgata acaaattagg tgatgccggt gcgcgcgtgg tcattgaaga
     2281 gtttttggtc ggcgaagaag ccagttttat ggtgatggtc gatggcaaaa acatcctgcc
     2341 cttagccacc agtcaagatc acaaacgcct gctggacgcc gaccaaggcc ctaacacggg
     2401 cggcatgggc gcttattctc ccgcacccgt ggtcacgcct gacatccacg ccaaagtgat
     2461 gcgtgagatc atcgtaccta ccgttgaagg catggctaaa gacggcattc cctacaccgg
     2521 cttcctctat gcaggcctca tgatcacgcc tgcaggtgac gtcaaaaccc tggaattcaa
     2581 ctgccgcatg ggcgatccgg aaacacaacc gatcatgatg cgcctgaaat cagacctggt
     2641 ggaattggct gagcacgccg tcaatggcac cctggaccag gccaaagctg aatgggactg
     2701 gcgctttgcc ttaggcgtag tgatggcaag tgcaaactac ccagagaccc caaagctggg
     2761 cgatgaaatt accggcctgc caaaagatgt gcaagatggc catgtgttcc atgctggcac
     2821 catactcaaa gatggcaaag tggtgaccag tggtggccgc gtactttgtg tgacagcgct
     2881 cggtgaaacc gtcaagtttg cacaacagca ggcatacaag atactggaac aaatccagtt
     2941 cgacggtgct cagtatcgta aagatatcgg ttaccgcgcc atcaaaaact aagacgtaca
     3001 aaagccaata gccatgacag agcagatcca accgcaagcc gtctttgatt acctgcaagg
     3061 gttacaggca cgtattgttg aggccatcga actggtcgac ggaaaaacct ttctgcagga
     3121 tagctggcag cgccccgaag ggggtggcgg caattcgtgc ctactcgaag gtggcaacgt
     3181 cttcgaacgc gccggggttg gattctcaca tgtcctgggt aacaagttac cgcctgcggc
     3241 aagcgtcgcc catcccgaag ctgctggcag gccttgggaa gccatgggcg tgtccctggt
     3301 ttttcatcca cacaatccct atgtccccac agttcacatg aatgtgcgct tctttgtcgc
     3361 caaggcacaa cacgctggag aacaggatat ctggtggttt ggtggcggca tggatctcac
     3421 accttattac ggttttgagg atgactgcat tcactttcac cgtgtgaaca aagaggctct
     3481 ggcaccattt ggcgcggact actatcgtca ctttaaaaaa gcatgtgata catactttta
     3541 cctcaaacac cgcaaagaac cgcgcggcat tggtggtgtg ttctttgatg attttagcga
     3601 gttagggttt gacaagagct ttgccttaca acgtgccgtt ggcgatgctt tcatcaatgc
     3661 ctatttgcca atcgtgcaac gccgtaagga tacggcttac ggtgagcgcg aacgtgactt
     3721 tcaggcttac cgccgtggcc gttacgtcga atttaacctg atttttgacc gcggtaccat
     3781 ttttggactg cagtcgaatg gccgtaccga atccatactg ctatccatgc cgcccgtggt
     3841 gaaatggcgc tatgactgga agccggaggc caccagcgca gaagccaaac tgtatagtga
     3901 ctttttgatt gagaaagact gggtgtaacc atgcatgttt cagtagaagc cagacaacaa
     3961 gcacaagcgt tactggattt tattgatcgt agccccagtc cctggcacgc agtagaaacc
     4021 gtggcggacc agctaaaggc acatggcttt aaaaacctgc gtgaaaacga accctggcag
     4081 tttaaacagg gaggcaaata ttttgtcgtg cgcgatggcg gctcaatcat tgcgtttgta
     4141 ttgggtcagg cgcccttagc tgacgatggc ttccgcatcg tgggggcgca tacagattct
     4201 ccaggcctac gcctgaaacc caaagcggct tacagcacag aaggtttagc gcagttaggt
     4261 gtggaagtct atggtgggcc catcctggcc acatttactg atcgagacct gagtctcgcc
     4321 gggcgcgtgg tgattcagac cgcgcagggc ttggaaagcc gcctggtcag aattgatcaa
     4381 gccattgccc gcctgcctaa cctcgcaatt cacatgaacc gcgaagtgaa cgaaaaaggc
     4441 ctggtgctca ataagcaaac cgggctacct ctgatttttg gccatgcagc caataccgat
     4501 agcgccaagc aattactcca tgaaacattg gcgggcaaac tggcgatccc tgcctccgcg
     4561 attgtcagtt gggatctcgc tttttacgat attcaaaaag gcagcttctg gggggtagaa
     4621 caagagttta ttgccaatag ccaactggac aatctggcct catgccatgc tgcactggcc
     4681 gcgctattaa gcaccgaaac gccgcaggcg acctctattt gtgcattgtt tgatcacgaa
     4741 gaagtcggct ctgaaagttc tactggcgcc ggcggcagct ttttgctgga tgtgattgag
     4801 cggatttgcc acagtacagg gctcacgcaa gaagacaagc tgcgcagttt tgccagtagc
     4861 ttttttatca gtgccgatat ggcacatgct ttccacccca atcatgccgg cagctatgaa
     4921 ccgtgccatc atgtacaggt taatcagggt ccggtcatta aaaccaacgc caaccagcgt
     4981 tatagcacca atgcagtcac ggctgcgcgc tttatccagt tatgcgaaaa agcaggcgca
     5041 ccctaccagc aatacgcaca ccgcacggat ttaggctgcg gcagtaccat aggcccaatc
     5101 atggcggcgc aactgggcat tgccacggta gatgttggca acccgatgtg ggccatgcac
     5161 agcattcgtg aaagcgcagg cgtgcttgac cataaccaca tgattgccac cttgcagcaa
     5221 cattacgcca gttaatcccg ccgcagcatt gacgctggta cagcgattgc tttaccctaa
     5281 gcaaattagg ggagaatcga ttaatgttaa agcaattatt gcgcaaaaaa ccaattcaac
     5341 cgcatgcgca tacaggcctg aaaaaatgtt taaccgcttt cgacctggct ttattaggca
     5401 tagggggcgc catcggcaca ggcatttttg tcttaaccgg cattgcagcc gcgacccagt
     5461 ctggcccagc agttgtgatt tcattcatta ttgccgccat cgccgccgct ttcgccgcac
     5521 tctcctatgc agaattgtcc tcctctattg gcggttcagg tagcgcctat ggctatagct
     5581 atgttgcctt tggcgaattt gccgcctgga tgatgggctg gatgttgtta ctcgaatatg
     5641 gtgtcggtgc tgccgcggtt gccaatggtt gggcaggcta ctttgtcaac accctcagta
     5701 accttggtct gcatttaccc gagcacctga ccaaagcccc ggtactgggc gggtcgatca
     5761 acctccctgc ttttgccatt atctggatat tgaccctact gctgatggta ggcgtgaagg
     5821 aaagtgcccg cgccaacaat atcatggtga ttatcaagct ctctaccatt gcgatcttcc
     5881 tggctttatc cgttggtcac attagcacct ccaactggca accattcatg ccgttcggct
     5941 ggtttgaaac cctcgataat ggcaagaata ttggcgtatt ggctggtgca tcactggtat
     6001 tctttgccta ttttggtttt gatgcagtct caaccgctgc cgaagagtgt cagcaacctc
     6061 agcgtgacct gcctattggc ctgattgcct cactgacctt ttgcacgatt atctatatta
     6121 ttgtttccgg cacgctcacc ggcgtcattc cctataccga actcaacacc tcatcccccg
     6181 tcgcttacgc attgaccaaa ctgggataca cctggtcatc caccctggtc gccacgggcg
     6241 tactggctgg tttgatcacg gtattactcg ttctactgta tggattgacc aggattttat
     6301 ttgccatgag ccgtgatggt ctgatttctc cagtattctc taccgtcaac ccggaccgcc
     6361 aaacgcccag caagatcatt ctcatgtgcg ggtttgtcgt gtctattgtc gccggattta
     6421 ttccgctggg agaactggca gaaacagtca atattggtac cttggccagc tttatcatgg
     6481 tctgtatcgg ggtaatccgc ttgcggcaaa cgcatccaga ccttccacgg ccgtttaaga
     6541 acccatttaa tccactgatt ccccttggtg gtgtggccag ttgtgctgca ctcatgtctt
     6601 ttttaccagc agaaacctgg catcgctttc tcacctg
//
LOCUS       DATMSK010000123         3200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_9357, whole
            genome shotgun sequence.
ACCESSION   DATMSK010000123 DATMSK010000000
VERSION     DATMSK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9357"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VNZ84_04170"
     CDS             <1..419
                     /locus_tag="VNZ84_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HWU66183.1"
                     /translation="HHDLKPLLQEADDREQLQQCWSAVAPEFSRLSHVLASYDGTLTI
                     AVQSGAVASRIRLMEAQLLRNIRDFCQNSRKIKGLNLSAIKVKVQVKSRPQSKHKRIK
                     PPSNRALNTLETYANEVNNPALETALRHFIDHQRHD"
     gene            583..2613
                     /locus_tag="VNZ84_04175"
     CDS             583..2613
                     /locus_tag="VNZ84_04175"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWU66184.1"
                     /translation="MSVALMLAIGAAVLAVLYGVVMSKWISGLPAGNARMQEIAGAIQ
                     QGAAAYLARQYKTIALVGVVLAILIGIFLSPQTAIGFVLGAVLSGACGFIGMNVSVKA
                     NVRTAQAATHGIGPALDVAFKGGAITGMLVVGLGLLGVTGFYIYLGAEQAADLNPLIG
                     LAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGD
                     CAGMAADLFETYAVTLIATMVLGGLMAAEAGASGVIYPLLLGAVSIVASIIGCFFVKA
                     SPGMKNVMPALYKGLAVAGVLSLIAFYFVTQYVFPQGYTHGEVTVSSDALFGACAVGL
                     ILTAALVWITEYYTGTDYAPVKHVAQASTTGHATNIIAGIGVSMKSTALPVISVCIAI
                     YVAFHLAGLYGVAIAAMAMLSMAGIVVALDAYGPITDNAGGIAEMSELPSSVRDVTDP
                     LDAVGNTTKAVTKGYAIGSAGLASLVLFADYTHKLEGAGITASFDLSDPKVIIGLFIG
                     GLIPFLFAAMAMEAVGRAAGSVVEEVRRQFREIKGIMEGTAKPEYGKAVDMLTSAAIK
                     EMMLPSLLPVAAPILVGIFLGPVALGGLLMGTIVTGLFVAISMCTGGGAWDNAKKYIE
                     DGHHGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLIVPLLH"
     gene            complement(2680..>3200)
                     /gene="ppa"
                     /locus_tag="VNZ84_04180"
     CDS             complement(2680..>3200)
                     /gene="ppa"
                     /locus_tag="VNZ84_04180"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWU66185.1"
                     /translation="DLPHDFNVIIEIPANGDPVKYEVDKESGALFVDRFMGTSMQYPC
                     NYGYIPHTLGDDGDPVDVLVITPVPLLPGVVVRCRALGMLKMTDEAGGDAKVLAVPIE
                     KVCGLYAYQKTLSDVSPWRLDMIAHFFEHYKDLDKGKWVKIEGWVGIEDAHKEITDGV
                     TRYNEAEVKPAF"
BASE COUNT          767 a          814 c          805 g          814 t
ORIGIN      
        1 atcatcacga cctcaagcct ttgttgcaag aggcggatga ccgcgagcag ctacagcaat
       61 gctggtctgc ggttgccccg gaattctcca ggctttcgca tgtgctggct tcatatgacg
      121 gcaccctgac gattgctgtg caatcaggcg cagtagccag caggatcagg ctcatggaag
      181 cccaactctt gcgaaacatc cgggattttt gccaaaattc ccggaaaatc aaagggttaa
      241 accttagcgc aattaaggtt aaagtgcaag taaaatctcg cccacaaagc aaacataaac
      301 gtataaaacc gccttcaaat cgcgctttaa acacgctgga aacctatgca aacgaggtta
      361 ataatccggc actggaaacc gcgttacgtc actttattga ccatcagcgt cacgattaat
      421 cctgctcttt ttcgatgcaa aaactgcgct aaaaacgtgc ttgtagacgc actttaatag
      481 tgcaataatg cccccaacca gttacatttt atttacaaat ccagtaacag gttaaacaga
      541 gtcattacgt tcaacgcagt ttcatcagga acaggagaag tcatgtcagt cgcactaatg
      601 ttagccatag gtgcagcagt tttggcagta ctctatggtg tagtaatgag caaatggatt
      661 tccggtttac ccgcaggcaa cgcacgcatg caagaaattg cgggggccat tcaacaaggc
      721 gccgcggcct atctggcaag acaatataaa accatcgcgc tggtgggtgt agtgctggcc
      781 attttaatcg gtattttctt aagcccgcaa accgccattg gctttgtgct cggcgctgtc
      841 ctgtcaggtg cctgtggctt tattggcatg aacgtctcgg ttaaagccaa tgtaaggact
      901 gcacaagcag ccacccatgg catagggcct gcactggacg ttgcctttaa aggcggcgcc
      961 attactggca tgttagtggt tggcttggga ttattaggcg tgactggttt ctacatttac
     1021 ctgggtgccg aacaagcagc tgacttaaac ccattgattg gcctggcatt cggctcttca
     1081 ttgatctcta tctttgcccg gcttggtggc ggaattttta ccaagggtgc agatgtcggc
     1141 gccgacctgg tcggtaaagt ggaagcaggc atccctgaag acgacccacg caaccccgcg
     1201 gtcattgccg acaatgttgg tgacaacgta ggcgactgtg caggcatggc ggccgacttg
     1261 tttgagactt atgccgtcac actgatcgct accatggtac ttggtggact gatggccgct
     1321 gaagcaggtg ccagcggtgt gatttatccg ttactgcttg gtgccgtgtc tatcgttgcc
     1381 tcaatcattg gctgtttttt tgtcaaagca tcgcccggca tgaaaaatgt aatgcctgcg
     1441 ctttataaag ggttagcggt ggctggcgta ctgtcattga tcgctttcta ctttgtgacc
     1501 caatatgtat ttccacaagg ctatacgcac ggtgaagtca ccgtttccag cgacgctctg
     1561 ttcggcgcat gtgcggttgg cttgatactt accgcagcgc tagtctggat tactgaatac
     1621 tatactggca ccgattacgc gccggtaaaa catgtggcac aagcgtccac gaccggccat
     1681 gcgaccaata ttattgccgg cataggggta tcgatgaaat ctactgcatt gcctgtcatt
     1741 tccgtttgta tcgcgatcta tgtcgccttt cacctggccg gcttgtatgg cgtagcaatt
     1801 gccgcgatgg cgatgctcag catggcaggc atcgtggtcg cgctggacgc ttacggcccg
     1861 atcacagata acgcaggagg cattgccgag atgtcagaat tacccagtag cgtgcgtgac
     1921 gtgacagacc cgcttgatgc cgtcggcaac actaccaaag ctgtcacaaa aggctatgct
     1981 attggttcag cagggctggc atctttggtg ctgtttgcag attacaccca caaactggaa
     2041 ggtgcgggca tcactgccag ttttgattta agcgacccga aagtaattat tggcctgttt
     2101 attggggggc tgatcccttt cctatttgcc gccatggcca tggaagccgt tggccgtgca
     2161 gcgggcagtg tcgttgaaga agtacgtcgc cagttccgcg aaatcaaagg cattatggaa
     2221 ggtacagcca aaccggaata tggcaaggct gtggatatgc tgaccagcgc cgccatcaag
     2281 gaaatgatgt tgccatcctt gctgcccgtc gcggcgccta ttttggtcgg catttttctg
     2341 ggcccagtcg cccttggagg cttattgatg ggcaccatcg tcaccggctt gtttgtcgcg
     2401 atttccatgt gtacgggtgg cggcgcctgg gacaatgcca agaaatacat cgaagacggt
     2461 catcacggcg gcaaaggctc ggatgcccat aaggctgcag tcaccggcga tactgttggc
     2521 gacccttata aagatacggc tggcccagcc gtgaacccgt tgattaaaat tatcaacatt
     2581 gtagccttgc tgattgtgcc cttactgcat taggcggagt tacaggtaat aaaaaagtcg
     2641 gctaaggccg acttttttat gatgctcaac gaacagcaat tagaacgcag gcttcacttc
     2701 agcctcgtta taacgggtaa caccatcggt aatttccttg tgcgcatctt cgatgcccac
     2761 ccagccttcg attttcaccc atttaccttt atccaggtct ttatagtgct caaagaagtg
     2821 tgcaatcatg tccaggcgcc aaggcgaaac atcgctcaat gttttctggt aggcatacaa
     2881 gccacatact ttttcgatag gcactgccaa cactttggca tcaccaccgg cttcatctgt
     2941 cattttcaac atacctaacg cacggcaacg cactacaaca cctggcaata atggtacagg
     3001 tgtgatcacc agcacatcta ccgggtctcc atcgtcgccc agcgtgtgtg gaatataacc
     3061 atagttacat ggatattgca tggatgtacc catgaaacga tccacaaaca gcgcaccact
     3121 ctctttatct acttcatatt tcaccgggtc gccgttggcc ggaatttcga taatgacgtt
     3181 aaagtcgtgc ggcaaatctt
//
LOCUS       DATMSK010000124         4576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_220471,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000124 DATMSK010000000
VERSION     DATMSK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220471"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..196)
                     /locus_tag="VNZ84_04185"
     tRNA            complement(120..196)
                     /locus_tag="VNZ84_04185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Pro,seq:ggg)
     gene            complement(302..733)
                     /locus_tag="VNZ84_04190"
     CDS             complement(302..733)
                     /locus_tag="VNZ84_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWU66186.1"
                     /translation="MLETQKVTLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFTQL
                     KPVKRRGNRRYYQHHEVLLIRKIRELLYEQGFTISGARNRLDGVDEKAEKPARSAAEI
                     SSPVSQNTQPLIPEDIRLPVVDVVALREQIQQVLQLLKVTA"
     gene            complement(748..1062)
                     /locus_tag="VNZ84_04195"
     CDS             complement(748..1062)
                     /locus_tag="VNZ84_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HWU66187.1"
                     /translation="MTLTKADLADLLFEQVGLNKREAKDMVEAFFEEVRNALEQGDSV
                     KLSGFGNFELRTKSERPGRNPKTGEEIPISARRVVTFHASQKLKLRVEEHYAEQPLQA
                     QA"
     gene            complement(1142..3490)
                     /gene="pheT"
                     /locus_tag="VNZ84_04200"
     CDS             complement(1142..3490)
                     /gene="pheT"
                     /locus_tag="VNZ84_04200"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWU66188.1"
                     /translation="MQFSEQWLRSMVNPSLDTAQLGHALTMAGLEVEELTPVAPAFSR
                     VVVAEILETAKHPDADRLQVCKVKVGEEVLQIVCGAPNARAGLKAPCALVGAELPGFQ
                     IKQAKVRGVESFGMMCSAKELGIATEANGILELPADAPVGQDIREYLNLNDHTYTLKL
                     TPNRADCLSIVGIARDVVAMTGAPMTVPEIVTAAIGSQLTKAVNLNAAAACPAYYGRV
                     IEGVNAQAKAPDWMVRRLERSGIRSISALVDITNYVLLELGQPMHAFDYALLQGDVQV
                     RFANAGEKLTLLNDTEVTLKADDLVIADGNGPVALAGVMGGKPSSVTDATQAIFLESA
                     FFTPDVMAGKARRLGLSTDSSYRFERGVDFGNTLNALERATALVLEICGGQAGTITAV
                     TAQLPVRKQVSLRHSYLRSILGIDISLTQVGQLLAQLGFVYTEANGVFNVSAPTFRFD
                     IAREEDLIEEVARLHGYEHIPALTPVAPQGMLVSPEATRSKHWLRDVMVNQGYQEVVT
                     YSFVDANWEHDLYGNNQPIALKNPIASNLSVMRSGLWGGLLDALDYNLKRQQARAMLF
                     ELGARYEKAAQSFVETLTLSGLVYGNVQPEQWSAETRAFDFYDAKAHVEALFAQPIRF
                     VVGQHPALHPGQSARIYLGDKAVGWLGKLHPQWQQQYGLTSACYLFELDAHAALATQV
                     PAYTEVSKFLPVRRDLAVIVDQNVSAQAMIDAIIAEQIPQLQTVKLFDVYHGKGVPEN
                     KKSLAFLVLMQDTCKTMVDEEVDTIVAQILNNWSSKFAASLR"
     gene            complement(3540..4541)
                     /gene="pheS"
                     /locus_tag="VNZ84_04205"
     CDS             complement(3540..4541)
                     /gene="pheS"
                     /locus_tag="VNZ84_04205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWU66189.1"
                     /translation="MADLNHLIAEAEQDFAACHDVPALENAKAKYLGKSGALTEALKS
                     LGKLTAEERPTAGAAINVVKQAVEQALNARRDGILAAAQAKQLASETIDVTLPARAQS
                     AGGLHPVTLTLRRVEELFHSIGFSVADGPEIETDFYNFTALNIPDNHPARAMHDTFYV
                     DSGNDSMEHVLRTHTSPVQVHYMENNTPPLKIIAPGRVYRVDSDATHSPMFHQVEGLW
                     VDEQISFANLKGVVQDFLQKFFERDDLTVRFRPSFFPFTEPSAEMDMSWNGGWLEIGG
                     CGMVHPEVLKHVNIDSNQYRGFAFGLGVERLAMLRYGVNDLRHFFNNDLRFLQQFIK"
BASE COUNT         1164 a         1200 c         1063 g         1149 t
ORIGIN      
        1 cttaaagacc aataaaataa ggcatataca aaaacaaaat gtatatgcct ttgtatatgc
       61 cttagcatga tttcagtaaa ttactggaga ttgatatcga ggtaagttat tgattttatt
      121 ggtcgggacg gtgtgattcg aacacacgac cccttgcacc ccatgcaagt gcgctaccag
      181 gctgcgctac gccccgacgt ttgactcgag caggaaccca ctcgaaaaga aggctgcatt
      241 ataaagcaaa accgcatatt cgctaagcag aatatgcggt tttataggct tgcaagctag
      301 ttcaggcggt gacttttaga agttgaagta cctgttgaat ttgctctctg agtgcaacga
      361 catcaaccac tggaagacgt atatcttccg gtatcagtgg ttgtgtattc tggcttaccg
      421 ggctggaaat ctcagcagct gagcgtgctg gtttttctgc tttttcatct accccatcca
      481 agcgattgcg cgcaccactg atggtaaaac cttgttcata caaaagctct cggattttac
      541 ggatcaacaa gacttcgtga tgttggtagt agcgacgatt accacgtcgt ttgacaggct
      601 tgagctgggt aaattcttgc tcccagtacc gcaagacatg tggcttaacc ccacacaact
      661 cactgacttc accaatggtg aagtagcgct tggctgggat aggcggcaac gtcacttttt
      721 gcgtttctag catggcgtgt gattagatca ggcttgtgct tgcagtggtt gttcggcgta
      781 gtgttcctcc acgcgcaatt ttaatttttg actggcgtgg aacgtcacga cacgacgtgc
      841 agagattggg atttcttcgc cagtttttgg gttacggcca ggacgttcgg atttagtacg
      901 caattcaaaa ttaccaaaac cggacagctt gacgctatca ccttgttcca gtgcattgcg
      961 aacttcttca aaaaacgctt ccaccatgtc tttggcttca cgcttgttca gcccgacttg
     1021 ttcaaacaac aaatcggcca agtcagcttt tgtgagtgtc attaacgatc ccctgaacct
     1081 tcttattatg cgagatgacg cttgtgttgt atcaactgtt atccttggtg ttacccaacg
     1141 actatcttag cgatgcagca aatttggatg accagttgtt gaggatctgt gccacgatag
     1201 tatccacttc ctcatcgacc atggtcttgc aagtatcttg cataagtaca agaaatgcaa
     1261 ggcttttttt gttttcgggc acacctttac catgataaac atcgaacaat tttaccgttt
     1321 gtaactgcgg aatttgctcg gcaataatcg catcaatcat ggcttgtgca gaaacatttt
     1381 gatcaacgat tacggccaaa tcgcgacgta ctggcaagaa tttgctgacc tctgtatacg
     1441 caggtacttg ggttgccaac gcagcatgtg catccagttc gaacaagtaa caggcactgg
     1501 tcaatccata ttgctgctgc cattgcggat gcaatttacc caaccagccg accgctttgt
     1561 cacccaggta aatacgggcg gattggccgg gatgcaaagc cggatgctgc ccgaccacaa
     1621 aacgtatagg ctgggcaaac aaagcctcca catgcgcttt agcatcataa aaatcaaagg
     1681 cacgcgtctc agcagaccat tgttcaggct gtacgttacc gtaaaccagg cccgacaagg
     1741 ttaatgtttc aacaaagctt tgcgcagcct tttcgtagcg tgcgcctaac tcaaacagca
     1801 tggcacgtgc ttgttgacgc ttgaggttgt aatccagggc atccagcaaa ccaccccata
     1861 atcctgaacg catgacgctc agattgcttg caattgggtt tttaagggcg attggctggt
     1921 tgttgccata cagatcgtgc tcccaattgg catccacaaa gctgtaggtc accacttctt
     1981 gatagccttg attgaccatc acatcgcgca gccagtgctt gctacgtgtc gcttcaggtg
     2041 aaaccagcat gccttgtggt gcgactggag tcagtgcagg gatgtgctca tagccatgca
     2101 agcgcgcgac ttcttcaatc aggtcttctt cgcgtgcaat atcaaacctg aaagtaggcg
     2161 cactgacatt aaacacacca ttcgcttcgg tatacacaaa tcccaactgg gccagcaact
     2221 gacccacttg tgtcaggcta atatcaatcc ccagaataga gcgcagataa ctatgacgca
     2281 ggctcacttg cttgcgtact ggcaactggg cagtcactgc agtgattgtg ccggcctgac
     2341 caccacaaat ttctagcacc agtgcagtcg cacgttccaa cgcgttcagg gtattaccaa
     2401 aatccacgcc gcgttcaaaa cgataagaag agtctgtgct caaacctaaa cgacgggcct
     2461 tgcctgccat cacatctggc gtaaagaaag cgctttcgag gaaaattgct tgtgtggcat
     2521 cagtcacaga gcttggttta ccgcccatca cacccgccag cgcaacaggt ccattgccat
     2581 cagcaatcac cagatcatcc gctttcagtg tcacttcggt atcattcagc aatgtgagtt
     2641 tttcaccagc gttagcaaag cgtacttgca catcgccttg cagcagcgca tagtcaaacg
     2701 cgtgcatagg ctggccgagt tcaagtaata cataattggt gatatcgacc aaggcactga
     2761 tactgcggat gccgctacgc tccaaacggc gcaccatcca atcgggcgcc ttggcttggg
     2821 cattcacacc ttcgatcacg cggccataat aagccgggca agctgctgca gcattgaggt
     2881 tcacggcctt tgtcagctga ctgccaatcg cagccgtaac aatctcaggc acggtcattg
     2941 gcgcgcctgt catggcaacc acatcgcggg caatgccgac gatgctcagg caatccgcac
     3001 ggttaggggt tagctttaat gtataggtat gatcgttcaa attcagatat tcgcggatat
     3061 cctgaccaac aggggcatct gctggcaact ccaaaatacc attggcttct gtcgcaatgc
     3121 ccagttcttt ggcagagcac atcataccaa atgactctac cccgcgcacc ttggcttgct
     3181 tgatctgaaa cccaggcaac tcggcgccta ccaatgcaca aggtgctttc aaaccagcac
     3241 gggcattcgg tgcaccacag acgatctgca gcacctcttc gcccactttt actttacaca
     3301 cctgcaggcg atccgcatcc ggatgcttgg cagtttccag aatctcagcc accaccacac
     3361 ggctaaaagc aggggctact ggcgtgagct cttccacctc gagaccggcc atggtcaacg
     3421 cgtgaccaag ctgcgcggta tccagtgatg gattcaccat ggaacgtaac cattgttcgg
     3481 agaactgcat atgtgtctaa ccctgtaaaa tcggtgttta aagtggacta tcgattgtgt
     3541 tatttaataa actgctgcaa gaaacgcagg tcattattaa agaaatgacg caggtcgttg
     3601 acgccgtatc gcagcatggc caatcgctct acgcctaagc caaaagcaaa gccacgatat
     3661 tgattgctat cgatattcac gtgcttgagc acctcagggt gcaccatgcc acaaccgcca
     3721 atctccagcc aaccaccatt ccagctcatg tccatttcag ccgaaggctc tgtaaacggg
     3781 aagaaagatg ggcggaagcg tacggttaaa tcatcacgct caaagaattt ttgcaggaaa
     3841 tcctgcacta cgccttttaa gttggcaaaa ctgatctgtt catccaccca caatccttcc
     3901 acctgatgga acatgggcga atgtgtcgca tcggagtcca cgcggtaaac acgacctggc
     3961 gcaataattt tcaaaggtgg tgtattgttt tccatgtagt gcacttgcac aggcgacgta
     4021 tgcgtgcgta acacgtgctc catgctgtca ttgccgctgt ctacataaaa agtatcgtgc
     4081 atggcacgcg caggatgatt atcggggata ttcagcgcag tgaaattata aaaatcagtt
     4141 tcaatttcag ggccatcagc cacactgaaa ccaatcgaat ggaatagctc ctctacacga
     4201 cgcagcgtga gtgtcaccgg atgcaagcca ccagcagatt gcgcacgtgc cggcaaggtc
     4261 acgtctatgg tctcgctcgc tagctgtttg gcctgtgcgg cggccagaat accatcacgg
     4321 cgcgcattca gcgcttgctc tactgcctgc ttcactacat taatcgcagc acctgcagtc
     4381 ggacgctctt cagcagtcag tttacccaag cttttcaagg cttcggtcag cgcgccagat
     4441 ttccccaggt atttggcttt ggcgttttcc agggcaggaa catcatgaca agccgcaaag
     4501 tcttgttcgg cttctgcgat taaatgattg agatcagcca tcgtgcgtat tcttcaagtt
     4561 ttaagtaaca ttaaaa
//
LOCUS       DATMSK010000125         9184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_103510,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000125 DATMSK010000000
VERSION     DATMSK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103510"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..574)
                     /locus_tag="VNZ84_04210"
     CDS             complement(158..574)
                     /locus_tag="VNZ84_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66190.1"
                     /translation="MFNFSKDNVENTTDRLAKKSDEAIEEIAAQVKSTANDLLDAVND
                     TADHTQDKAKELIHSLKANIDRLTNGENAAAIASNLSSKAGRVKDQVQREVSETYQSL
                     KGKTVDTVQEHPLGTVLVAAGVGLLIGYLLGSKRRE"
     gene            874..2298
                     /locus_tag="VNZ84_04215"
     CDS             874..2298
                     /locus_tag="VNZ84_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWU66191.1"
                     /translation="MHKVRAYLIAALDRIEKVSDTFGGNTVALLTVSLLLAAAMLVVN
                     DRFIVTTKEKTDVLSMLDELLIETWKVQSNLAIAESSQRGFLLTRSDNYFKPYDDATG
                     QISQQLQTMNTLLNHTNLVKHVQIQATMSELMSSIAKKSAEMSVTIDFAKKRQFDKAL
                     AVVKLDSGIKESRNIEVLSRRFEQQVMDYRLELEDNRNTLRRMMRWFVMACPLIFVSL
                     VVLVIRRLLRELAEKANLYVRLTDENAEYERNIQTLTTTLQRQALQAQTNSERERYQL
                     SRELHDELGSLLTAIKMDISWTIKHLKDDRPQVTEKLKKTNQYLDRSINFKREIVQNL
                     HPSMIKSFGLIASLQNLVNEAASRNQWQMDIIMPEQEMAINETLSLIIYRLVQESLNN
                     CSKYAKATQVSVHLLHDDHYIKLEVMDDGIGFDMNNLTSANTGISGMRNRVESIGGQY
                     EVTSAPQQGASMRVMLPFLKVDSL"
     gene            2429..2944
                     /locus_tag="VNZ84_04220"
     CDS             2429..2944
                     /locus_tag="VNZ84_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWU66192.1"
                     /translation="MLMSKYKQATIFGGLLATTITLNAFAFDLQEVQNGDKPYLEEFT
                     ALDKNANSTLTWAEVQQDKGVKQQDFKAYDHDKDGTLSYEEYAELKTQTGQKAIKGAM
                     SDSWITTKVKARLLEEESLKSLKISVETHKGEVLLSGFVGDASLKTKAEELVASVEGV
                     KSVKNAIVVKG"
     gene            3090..3500
                     /locus_tag="VNZ84_04225"
     CDS             3090..3500
                     /locus_tag="VNZ84_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWU66193.1"
                     /translation="MNKMNRYLISSVFSGILLVASTQVMAKGEPMYNFLPSDTVVDNV
                     KSTLQNHGIDVSAIQVNVDAQGVVQLTGEVDSKQQVEKITQLAKEADGVYAVLGELRY
                     ETGEVVPAPMPEGMDPTMESPAAGNENHSQVEMQ"
     gene            complement(3546..4073)
                     /locus_tag="VNZ84_04230"
     CDS             complement(3546..4073)
                     /locus_tag="VNZ84_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HWU66194.1"
                     /translation="MPPAAEQAIVHSFDPAQYIGTWYEIARLDNTFEKGLEQVTADYS
                     IQPDGSLKVINRGFNPEKKAWSEAVGKAKFVDPANPDGSYTGRLKVSFFGPFYGDYHI
                     LELDKPYYNYALVSSGRDYLWILSRTPQLTYPIKQHLMAKAKALGFATDQLLFIRQAN
                     QVEYESGRHVMPEHH"
     gene            4232..4672
                     /locus_tag="VNZ84_04235"
     CDS             4232..4672
                     /locus_tag="VNZ84_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HWU66195.1"
                     /translation="MKFLNGFKVCAALVLLSTQVMAGNLETGLGGGVGAAAGTAVGSV
                     LGGSTGEVIGGAVGGGLGGAATTGRSGRTGAILGGAAGGATGAMVGRQVSGTTTGAVV
                     GAGVGGAGGAVVGKVIDEPDPYERRYRGKKGKKHGHGRGHHRHD"
     gene            4706..5146
                     /locus_tag="VNZ84_04240"
     CDS             4706..5146
                     /locus_tag="VNZ84_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HWU66196.1"
                     /translation="MRAWILGVSLCCMSVLAQAQSCNNKNVAASGVGGVAGSAIGAVM
                     LGPAGEVLGGAVGGAVGSVVANGGKTQPKTMLGGAVGGAGGAILGRGLGGGRLLAVLG
                     AGLGSAGGACVAQQASAGNTTHHGRAKKHSVSNATAKAQKLLGS"
     gene            complement(5186..6160)
                     /locus_tag="VNZ84_04245"
     CDS             complement(5186..6160)
                     /locus_tag="VNZ84_04245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWU66197.1"
                     /translation="MQAPATPANEVERLHVLRALAILDTQAEERFDRLTRIATRMFNV
                     PISLVTLVDSNRQWFKSCMGLQVKETPRDISFCGHAILGNDAFVINDASKDPRFSDNP
                     LVTGVPHIRFYAGQPIRTTHGFKIGTLCLIDSQPREFPARDVEDLTDLACMVENELAA
                     IELATMDELTDINNRRGFTMLTEKYLNYFTRHHVFASLVFLDLNGFKLINDTYGHDEG
                     DRALQTFGKALKQVGRTSDIFARLGGDEFVVLLANTRQHEAHIYIQRLVTHLDTLRQQ
                     QRQPYKISFAQGIVEFDPQLPKSVEQLLLEGDALMYQLKKLAQKLASK"
     gene            complement(6280..6603)
                     /gene="sugE"
                     /locus_tag="VNZ84_04250"
     CDS             complement(6280..6603)
                     /gene="sugE"
                     /locus_tag="VNZ84_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HWU66198.1"
                     /translation="MTWLILVIAGLFETAWAIGLKYTDGFSKFWPSFWTILAMVISVW
                     LLGLCVKDLPVGTAYAVWVGIGAVGTVIGGIYLFSEPVSSLRLISLCLIIAGIIGLKL
                     ATTHP"
     gene            complement(6657..7373)
                     /locus_tag="VNZ84_04255"
     CDS             complement(6657..7373)
                     /locus_tag="VNZ84_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HWU66199.1"
                     /translation="MAKTLDLERILHRQGFGTRKMCRILIINEEVTVNGELCDDPDAK
                     FALDDNLHFTVQGEPWQYREHSYLMMHKPAHYECTHKTQFHPTIYTLLPAPLVERGIQ
                     CVGRLDEDTTGLILLSDDGQFIHKVSSPKHHVPKVYEVTCKHPVSDADRQHLLSGVKL
                     LDEEETIAALAVTSLSDTVIHMTLAEGKYHQVKRMVAAIGNRVEGLKRIKIGQLTLPT
                     ALAEGEWRWLTAEDLQAVQS"
     gene            7488..8276
                     /locus_tag="VNZ84_04260"
     CDS             7488..8276
                     /locus_tag="VNZ84_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWU66200.1"
                     /translation="MAFKHITSRDNPVFKQLRKLVDQSRERKKTASTLLEGVHLIEAY
                     LQVYGEPRLLIIPEGQSSVEATALIQQLADVDTMLLPTLMFAELAPVASSSGVMALVD
                     IPEAELPETVDFALLIEDIQDPGNLGSMLRTAAAAGVQVAYLTKGCTDAWSPKALRGG
                     QGAQFVLPVVEGVDAVEIVNGFAGLTLALTMQGDSLYQQSLRESVLFAVGNEGAGISG
                     ALQSAVNTCITIPMAHQGTLALESLNAAAATAVALFECQRQRLN"
     gene            complement(8283..9155)
                     /gene="hslO"
                     /locus_tag="VNZ84_04265"
     CDS             complement(8283..9155)
                     /gene="hslO"
                     /locus_tag="VNZ84_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HWU66201.1"
                     /translation="MTKTLSTDQLHPFIFEQSAVRGNCVHLDQSLFSALEHQTLPPPL
                     KKAIGEFTAASVLLTATLKMQGKLILQLQSQGSLQLLVVECTSDLNIRATAKVHGELL
                     GDSLTDWLKQGQLVITLMPEEGEPYQGIVPLEGDNIASMLENYMLRSQQIDTRLWLTA
                     EGDRAAGLLLQKLPQQQEQDLDCWNRVQHLAGTVTDPELLELDTTTLLQRLFVEEDVR
                     LFSGRQIQAFCSCSQTNVTNMLQMLGLAEVRSILEEQGSIEIQCDFCHKHYVVDEEEA
                     LALFNDDDDHEVTH"
BASE COUNT         2351 a         2116 c         2392 g         2325 t
ORIGIN      
        1 ctacaaaatc ctgttttaat tgctgtgcac ggtcctctgt atagtcggcc acgtgatggc
       61 gcagattgaa aagattatct ttcagcgccg ccaccttgcg attcagaaat aaaacagcag
      121 caaatttcaa gaggtttacc cacatagctt tcctagctta ttcacgacgt ttggaaccta
      181 gcaggtatcc tatcagtaaa cccacgccgg cggctaccag tacggtgccc agagggtgtt
      241 cttgtacggt atccactgtt tttcctttga gggactggta agtctcgctc acttcacgct
      301 gaacctgatc tttcacacgg cccgcttttg aggacaggtt ggaagcaatt gccgcagcat
      361 tttctccatt ggtcaggcga tcgatgttcg ctttcaggct atggataagc tctttagcct
      421 tatcctgggt atggtctgca gtgtcattga ctgcgtcgag caaatcattt gcagtagact
      481 taacctgtgc tgcaatttcc tcaatggctt catctgactt tttagccaaa cggtcagtcg
      541 tattctcaac attatctttg gaaaagttaa acattttgaa actccttttt tcgtgattac
      601 aaaagaaaac accctgtttc ttagacagtt caatgggttg aaagtgtttt cagcatgtgc
      661 ccatcttacg taatgcgtgg ctgtgcccct atcggccacc caccgatatc aaggtcagaa
      721 tcttctgaca aacctgaaaa tgcgtaacgc caagcaggtt tacgttgctg atcatgagta
      781 acatcgttat gatgccgcta atacattttt aacaatacat ataaaaaacg ttactctgga
      841 tggtctggat tttcaatctt tcatttggct gccatgcata aagtcagagc ctatcttatt
      901 gccgcgcttg accgcatcga aaaagtcagt gatacctttg gtggtaacac agtggctttg
      961 cttaccgtga gtttgttact ggcagcagcc atgctggtgg tcaacgaccg ttttatcgtc
     1021 actaccaaag aaaaaacaga tgtactgtcc atgttggacg agttgttgat cgaaacctgg
     1081 aaggtgcaat ccaacctggc gattgccgaa agttctcaac gtggcttcct gctcacccgc
     1141 tcagataatt attttaagcc ctatgacgat gcgaccgggc aaatcagcca gcagctgcaa
     1201 acgatgaata ctctgctaaa ccatactaat ctggtcaagc acgtgcagat acaagcaacc
     1261 atgagtgagc tgatgagcag catcgccaag aagtcagccg agatgagcgt caccattgat
     1321 tttgccaaga aacgccaatt cgataaggct ttggcggtgg taaaactgga ttctgggatc
     1381 aaggaaagca ggaatattga ggtgttatcg cgccggtttg agcagcaggt aatggattac
     1441 cggcttgaac tcgaagataa ccgcaacacc ttgcgtcgca tgatgcgctg gtttgtgatg
     1501 gcatgcccac tcatttttgt gtcgctggtg gtgctggtga tccggcgctt gttgcgtgag
     1561 ctggcagaga aagccaatct gtatgtccgc ttgacggatg agaatgcgga atatgagcgc
     1621 aatattcaaa ccctgactac cacgttgcaa cgccaggcat tacaggcgca aacaaatagt
     1681 gagcgcgaac gctaccagct ctcaagagaa ctgcatgatg aacttggttc tttgctgaca
     1741 gcaatcaaga tggatatttc gtggacgatc aagcatttaa aagatgatcg gccgcaagtc
     1801 acagaaaaac tcaagaaaac caatcagtat ctggaccgca gtattaattt caaacgcgaa
     1861 attgtgcaga acctgcatcc gtccatgatc aagagctttg gtttgattgc ttcgttgcag
     1921 aatctggtaa atgaagcagc gtctcgtaat caatggcaaa tggatatcat catgccagag
     1981 caggaaatgg cgattaatga gacattaagc ctgatcattt accggctggt gcaagaaagc
     2041 ctgaataact gcagcaagta tgccaaagcc acacaagtgt cagtgcactt gttgcacgat
     2101 gatcactaca tcaagctcga agtgatggac gatggcattg ggtttgatat gaataacctc
     2161 accagtgcca ataccggtat cagcggcatg cgtaaccgcg tggaatccat tggtggccag
     2221 tacgaggtga ccagtgcgcc acaacaggga gccagcatgc gtgtcatgct cccgtttctt
     2281 aaagtagata gcctttaatt tcgccttgta gtcatattct gacaacggat tcagtatttg
     2341 gacgactgac tgacactcct tccctcatta acatggacac atgcaaaaca gacacacgtt
     2401 ttgcgaaagc gtgaaaaagg agacgcacat gttaatgtca aaatacaaac aagcaacaat
     2461 ctttggcggc ctgctggcga caaccatcac attaaacgct tttgcgttcg atcttcagga
     2521 agtccaaaat ggtgacaaac cttacttaga ggaattcact gcattggata aaaatgcgaa
     2581 tagcaccttg acttgggctg aagtgcagca ggacaaaggc gtgaagcaac aggatttcaa
     2641 ggcatatgac catgacaaag atggcacatt aagttatgaa gagtatgccg agctcaaaac
     2701 ccaaactggc caaaaggcta tcaaaggcgc gatgtctgac agctggatca ccacaaaagt
     2761 gaaagcccgc ttgctggaag aagagtcact gaaatccctc aaaatcagcg tggaaacgca
     2821 taaaggtgaa gtgctgttga gtggttttgt tggtgacgca tccttgaaga caaaagctga
     2881 agagttggtc gccagcgttg aaggcgtcaa gtctgttaaa aatgcgattg tagtgaaagg
     2941 ctaaataaac acccccttgc aagctacgtt attctctccc aagtgtgtag cttgctcttt
     3001 tttttctggc gggttgttca caagtgttga cggattcatc ctctgtgact cgaacaactc
     3061 gccgccctaa ccaacagaaa ggaaacatca tgaacaagat gaatcgttat ctgataagca
     3121 gtgtttttag cggtattctc ttggtagcca gcactcaggt gatggcaaaa ggtgagccta
     3181 tgtacaattt cttgcctagc gataccgtgg tggacaatgt taaaagtacc ctgcaaaacc
     3241 atggcattga tgtctctgcc attcaggtca atgtagatgc gcaaggcgtg gtgcaattga
     3301 ctggtgaagt tgactccaaa cagcaggtag aaaaaatcac ccaattggcc aaagaggctg
     3361 acggcgtcta tgcggtgcta ggtgaattac gttatgaaac tggtgaagta gtgccagcgc
     3421 cgatgccaga aggtatggat ccgacaatgg agtcccctgc tgccggcaat gaaaaccata
     3481 gccaggtaga gatgcaatga gctgaccggg agacaatgtt ctcccgggga ttgaccaggc
     3541 aggaattagt gatgctcggg catcacatgt cgaccggatt cgtactcaac ctggttggct
     3601 tgacggatga acaatagctg gtcagtggca aatcctaatg ccttggcttt agccatcagg
     3661 tgttgtttga tcggataggt gagttgcggt gtgcgggaga gtatccataa ataatcccgg
     3721 ccgctgctta ccaaggcata gttatagtag ggtttgtcca gttccagaat atggtaatcc
     3781 ccataaaacg gtccaaaaaa tgatactttg aggcgaccgg tataactacc atccggattt
     3841 gccggatcaa caaattttgc tttgccaacc gcttccgacc atgctttctt ttcaggattg
     3901 aacccccgat taatgacttt aagcgagcca tccggctgga tgctgtaatc tgcagtgacc
     3961 tgctccaatc ccttctcaaa tgtattgtcc aaccgtgcga tttcatacca ggtgcctata
     4021 tattgtgcag gatcaaaact atgcacgatt gcctgctcgg ctgcaggtgg catggctttt
     4081 gaacaggcag tcaaagtaag caggatcaga ctagaaaaca ggaaaggtaa gcggtgaatc
     4141 cacacaagag tcagggtcat aagctattaa taaaaataat acgatttcta cagacagttt
     4201 aaaataacac aaattcaatc aagggaagaa aatgaagttt ttgaatggtt tcaaggtatg
     4261 tgcagcgctc gtcctgttgt caacgcaagt catggccggt aatttggaaa ccggcttggg
     4321 aggcggtgta ggtgccgccg cgggcacggc tgtaggcagt gtcctgggcg gtagcactgg
     4381 cgaggtgatt ggtggtgcgg taggtggcgg tttgggtggc gcagcaacga ctggccgctc
     4441 tggccgtact ggtgcgattt taggtggtgc ggcgggtggc gcgacaggcg caatggtagg
     4501 ccgtcaagtc agtggtacca ccacgggcgc cgttgtgggt gctggggttg gaggtgctgg
     4561 tggtgctgtg gtgggtaaag tgattgatga gcccgatcct tacgagagac ggtatcgcgg
     4621 taaaaagggt aaaaagcatg ggcatggccg tggtcatcat cgccatgatt aatcaaaaaa
     4681 gatagacaac agacggagct gtgtgatgag agcttggata ttgggcgtga gtttgtgttg
     4741 catgagtgta ttggcgcaag cacaaagttg taacaataaa aatgtcgctg ccagtggtgt
     4801 cggtggcgtt gccggttctg cgattggtgc cgtgatgcta gggcctgccg gcgaggtgct
     4861 gggcggagcc gtaggtgggg ctgtgggtag tgtggtcgcg aatggtggca aaacccagcc
     4921 taaaaccatg ctcggcggtg cggtgggcgg cgcaggtggc gcgattctgg gacgtggcct
     4981 aggtggtggc agattattag ctgtactagg tgctggtttg ggcagtgcgg gtggcgcttg
     5041 cgtagcgcaa caggcctcgg ctggtaatac tacgcatcat gggcgtgcca aaaaacactc
     5101 agtcagcaac gcaacggcta aagcccagaa actgttagga agttaaacct accgtttgcc
     5161 tgggaagaag tgattctgac gcaggctatt tgctcgccag cttttgcgct aactttttga
     5221 gttggtacat caaagcgtct ccctctagca ggagctgctc taccgatttt ggtaattgag
     5281 gatcaaactc cacgatgcct tgggcgaatg aaatcttgta aggctggcgc tgttgttgcc
     5341 tgagggtgtc aaggtgggtg accagccgct gtatgtaaat gtgcgcctca tgctggcgag
     5401 tattggccag taatacgaca aactcatcac cacccagccg ggcaaagata tccgaagtgc
     5461 gacccacctg ttttaatgct ttgccaaacg tctgtaaggc gcggtcacct tcatcgtgac
     5521 cataggtgtc attgatcaat ttgaaaccat tgagatccaa aaacaccaga gacgcaaaca
     5581 catggtgacg cgtaaagtag ttgaggtact tctccgtcaa catggtaaat ccacgccggt
     5641 tattgatatc ggtgagttca tccatggttg ccagctcaat ggctgcgagt tcattttcga
     5701 ccatgcaggc aagatctgtc aggtcttcca cgtccctggc tggaaattcc cggggttgtg
     5761 aatcaatcag gcatagtgtg ccaattttga aaccatgcgt cgtacggata ggctggccag
     5821 cataaaaacg tatatgcggt acaccagtca ccaatgggtt atcagaaaat cgggggtctt
     5881 tagaagcatc attgatcaca aaggcgtcat tgcccaggat ggcatgacca cagaaggaaa
     5941 tgtctctagg ggtttccttc acttgcaggc ccatgcagga tttaaaccac tggcgattgc
     6001 tatcgaccag ggtaaccagc gaaatgggca cattaaacat tcgcgtggca attctggtta
     6061 aacggtcaaa acgttcttct gcctgggtgt ccaggatagc cagagcgcgc agtacatgca
     6121 gacgctctac ttcatttgca ggggttgctg gtgcttgcaa agtagtgaat ccactcaagt
     6181 atccgaatgg gcatcatacc gcgattctgt gctgagtgta caaaaaaata cacttgtaat
     6241 tttgtgtttt gtatttgcgt tcgtttgcag agggatctat tagggatgtg tagtggccag
     6301 tttgaggcca ataatccctg caatgatgag gcacaggcta atcaggcgca gtgaagaaac
     6361 aggttcgcta aagaggtaaa tgcccccaat gactgtccca actgcaccta tgcctaccca
     6421 gaccgcataa gccgttccta ctggtaggtc cttcacacac agacccagaa gccagacact
     6481 gattaccatg gccagaatgg tccagaacga aggccagaat ttggaaaagc catcggtata
     6541 tttgaggcca atggcccacg cggtttcaaa taaaccagca atcactaata tcaaccaggt
     6601 catgaagttc cttttatggt ctgtaaaata tccctgagag attgagcagc aggccgttat
     6661 gactggaccg cttgcaaatc ctcggcggtt aaccagcgcc actctccttc agccaaggct
     6721 gttggcaagg ttaactgccc aatcttgatc cgctttaatc cttctacgcg gttgccgatg
     6781 gccgccacca tacgcttgac ttgatgatat tttccttcgg ctagtgtcat atgaatcact
     6841 gtgtcagaaa gacttgtcac ggccagcgca gcaatcgttt cttcttcgtc tagcagtttg
     6901 accccagaca ataggtgttg cctgtcagca tcactgaccg ggtgtttgca ggtgacctcg
     6961 tataccttgg gtacatgatg cttgggggag ctgactttat ggatgaattg cccgtcatct
     7021 gaaagtaaaa tcaagcccgt cgtgtcctca tccaatcggc ctacgcattg tatgccgcgt
     7081 tctaccagtg gcgcaggcaa cagcgtatag atggtcggat ggaattgtgt tttgtgcgtg
     7141 cattcataat gcgcaggttt atgcatcatt aggtagctgt gttcacggta ttgccaaggc
     7201 tcaccttgca cagtgaagtg caggttgtca tccagcgcaa acttggcgtc agggtcatcg
     7261 caaagctcac cgttcacggt gacttcttcg ttgatgatca agatgcggca cattttcctg
     7321 gtgccaaagc cttgcctatg cagaatacgt tctaaatcta gtgtttttgc catgtctgaa
     7381 ttatacgtga attgctcaag agagatggcg gcggataggt aattcaaaga ccatacaatt
     7441 atcgtcacgg gcaaaatcag ccccatcaat tcacgtataa tcctgtcatg gctttcaaac
     7501 acatcacctc ccgcgataac ccggttttca aacaattacg caaactggtt gaccagtcgc
     7561 gagagcgtaa aaaaacagcc tctaccttgc tggagggcgt tcatttaatt gaagcttacc
     7621 tgcaagtgta tggtgagcca cgcttgttga ttattcccga aggacaaagt agcgtggagg
     7681 cgacggcact tatccagcaa ttggcagatg tggataccat gctgctgcca accctgatgt
     7741 ttgcagagct cgccccggtg gcatccagca gtggcgtcat ggcgctcgtg gatattcctg
     7801 aagctgaatt acctgaaacc gtcgattttg ccttgttgat tgaggatatc caggatccgg
     7861 gtaatctggg gagtatgcta cggaccgcgg ccgcagcggg cgtgcaggtg gcctacctga
     7921 ccaaaggatg cacggatgcc tggtcaccca aagccttgcg tggcggacaa ggtgcacagt
     7981 ttgtcttgcc ggtggtcgag ggtgtagatg ccgttgagat tgtgaatggc tttgccgggc
     8041 ttacactggc tttaaccatg cagggcgata gcttgtatca gcagtctttg cgtgaatcgg
     8101 tattgtttgc ggtgggtaac gaaggtgcag gcatttctgg cgctttgcag tcagcggtga
     8161 atacctgtat caccattccc atggcgcatc agggcacgtt ggcgctggag tcactcaatg
     8221 ctgcggcagc cactgcggtg gccttgtttg agtgtcagcg ccagcggttg aactaagaat
     8281 gtctagtgcg tgacctcatg gtcatcatcg tcattaaata aggctaaagc ctcctcctca
     8341 tcgaccacat aatgtttgtg gcagaagtca cattgaatct caatgctgcc ttgctcttca
     8401 agaatgctgc gcacttcagc caggcctagc atttgcagca tattggtcac attggtttgt
     8461 gagcagctac aaaaagcctg aatctgacgg ccactgaaca aacggacgtc ctcttcgaca
     8521 aataagcgtt gtaacaaagt ggtcgtatcc agctcgagta actctggatc agtgactgtc
     8581 cccgccaaat gttgcacgcg gttccagcag tcgagatcct gctcttgttg ttgcggtaat
     8641 ttctggagta gcaaaccagc agctcggtca ccttcggcag tcagccacag gcgtgtatca
     8701 atctgctggg aacgcagcat atagttctca agcatgctag caatattgtc accttccagt
     8761 ggaacgattc cctggtaggg ctcgccctct tccggcatca aggtgatgac cagttgcccc
     8821 tgtttcagcc agtcagtcag tgaatcaccc agcagttcgc catggacttt ggccgtggca
     8881 cgaatgttca ggtcagacgt gcattcaacg accagcaatt gcagggaccc ctggctttgc
     8941 agttgcaaaa tcagtttgcc ttgcattttc agggttgcgg ttaataacac actggccgct
     9001 gtaaattcgc cgatggcttt tttgaggggc ggcggtaaag tctgatgctc cagtgcgcta
     9061 aacaggcttt gatccagatg tacgcagttg ccgcgcaccg cagattgctc gaaaataaaa
     9121 ggatgtaatt ggtccgtgct aagtgtcttt gtcatggtgc tattttaaca ttgaagcctg
     9181 tctt
//
LOCUS       DATMSK010000126        11555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_268000,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000126 DATMSK010000000
VERSION     DATMSK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268000"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VNZ84_04270"
     CDS             complement(<1..1092)
                     /locus_tag="VNZ84_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HWU66202.1"
                     /translation="MTLTQTLFTRRMLMCVALGFSSGLPLYLLLQLLPAWLRSEGLNL
                     KTIAAFAFMQLPYTWKFLWAPLMDRYSLLQALGRRRSWILATQLALFASIVGLGMCQP
                     QLDIAMVALLATLVALFSASQDIVIDAYRRETLSDEELGLGNSLYVNAYRIAGLVPGS
                     LALILADRMAWSQVFLIVGLFMLPGIGFTLWAKEPVITSVPKTLLASVVEPFKEFMQR
                     SGWQHALWVLLFILLYKLGDSMATALATPFYIDMGFSKTDIGAIAKGSGLVMQIAGAF
                     IGGLWMLKLGINRGLWVFGVVQALSILGFAWLATSGPFTDIGATERGMLAIVIGFEAF
                     GVGLGTAAYVAYMARETNPAYTATQLALFT"
     gene            complement(1296..1616)
                     /locus_tag="VNZ84_04275"
     CDS             complement(1296..1616)
                     /locus_tag="VNZ84_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWU66203.1"
                     /translation="MQLFNKTLLATALLAVTASAIAGSGAEALAQKSGCLLCHSVDKK
                     VLGPAYKEVAAKYKGDKSAEAKLIQKVKAGGAGVWGDMPMPPNSPQVKDEDIKTLVQW
                     VLSL"
     gene            complement(1630..3498)
                     /locus_tag="VNZ84_04280"
     CDS             complement(1630..3498)
                     /locus_tag="VNZ84_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HWU66204.1"
                     /translation="MQQRIKLAIGAMLLGSISTSAWANAELDAMIKDDNQWAMARKDY
                     ANQGYSRLSQIKKSNVKQLKLAWSFATGVNRGHEGAPLVIGNTMYIHTAFPNNVYALD
                     LDDNQKIAWSYFPKQDPDVQSVLCCDNVNKGLGYGDGKIFLQQNNGVLVALDAKTGAK
                     LWDVKVNDPKMGSANTNVPHVFKDKVVTGCSGGEFGVRCHISAYNIKDGSLAWKAYST
                     GSDSEVLIGADFNKENPHYSALSVYEDVNGGNKMGGSFKSLDKAQLKFPEANLGVRTW
                     LKPQQQKDGWQNGGGPTWGWYSYDPKLNLMYYGTGNPGTWNPDVRPGDNKWSMTIFAR
                     DLDTGMARWGYQMTPHDEWDYDGMNEIILWEANGKQLASHFDRNGFGYTFDRTNGTLL
                     VAEKMHPFVNWATKIDLKTGIPVKDPKYSTHQDYNAKGVCPSALGVKDEQPAAYSPKN
                     KTFYVPLNHVCMTYEPVESKYVAGQPWVGATLTMFAGPDGVMGGFMAWDGLKGKAQWY
                     NKEKFSAWGGALVTASDLVFYGTLDRWVKALDAKTGKELWKFQVGSGVVGNMITYGHK
                     GKQYVGVLSGIGGWAGVAMNLGLTNDTDGLGAAGGYKELTKYNAAPGGGAMNVFSL"
     gene            3767..5677
                     /locus_tag="VNZ84_04285"
     CDS             3767..5677
                     /locus_tag="VNZ84_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HWU66205.1"
                     /translation="MPNKYSPSTAQTSQPGLRDDQRAIAEAIARSWRRCMVKGVDEQA
                     PVEDQIITAQELNQRLEQNRLLLTHAEPEMLTLSEQIAHTRSLVILTDSEGIILRTMG
                     EGVEENEIRASLLPGASWSEEHRGTNAIGTALVERLPISVQGTEHFMAYHHSLSCSAV
                     PIFAANNHLVATLDVSNDLNAPQQHTLALVKMAAQMVENRLFHATAEGEIGVHFHVRP
                     EFIGTLWEGVALFDTQGQLQAINRSGQFQFGLPLDQDVLSARRIQFEDIFDESWTVFK
                     QRGLSASMLFPLRLRNGARLFARASATQAPLINSKQPIPSITRESAASLELLDSGDPQ
                     LKLAISQVKQVLNRDIPILILGETGAGKELFSRAIHDASARRDKPFIAVNCAALPEGL
                     IEAELFGYEEGAYTGAKRKGNIGKIQQAEGGTLFLDEIGDMPLSLQARLLRVLQDRSV
                     TPLGSSKSIPVNFMLVSATNQKLKDKVAAGEFRSDLYYRINGLAVQLPALRQRQDMAR
                     LIQMILQIEQAPQVTLSEEVMALFATHPWPGNVRQLHNVLRTAVALAEGGVIQRQHLT
                     HDFLDEMAVCSELVATAVVPTSVTGNLKAQSEAAIRQAMLQHSGNISAVARQLGISRN
                     TLYRRLKELDLA"
     gene            complement(5674..6276)
                     /gene="metW"
                     /locus_tag="VNZ84_04290"
     CDS             complement(5674..6276)
                     /gene="metW"
                     /locus_tag="VNZ84_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HWU66206.1"
                     /translation="MSVKNTNIRPDFALITNWVSTKAKVLDLGCGDGTLLTHLHETLE
                     TTGYGIEKDDANWLAALKNGVDVIQMNLEEGLSGFEDQSFDTVILSQTLQAMHNTESI
                     VQEMLRVGREAIVTFPNFGYWRNRMQITFGTMPVSKSLPYQWYDTPNVHLCTINDFDQ
                     FCVQHQIQVIERKVITGGQDVHILPNLMGNLAMYRLKKAA"
     gene            complement(6273..7400)
                     /locus_tag="VNZ84_04295"
     CDS             complement(6273..7400)
                     /locus_tag="VNZ84_04295"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HWU66207.1"
                     /translation="MSESNSVGIVKAQVAHFTQPLTLKSGAVLPQYHLTYETYGELNA
                     DKSNAVLICHALSGNHHVAGRYSPEDKYPGWWDNLIGPGKPLDTNKFFVIGLNNLGGC
                     HGSSGPSSVNPLTDRPYSATFPVVTVEDWVESQARLLDYLGIDQLAAVIGGSLGGMQA
                     LQWNIAYPERVRHAFVIASAPNLTAQNMAFNEVARQAIITDPEFFDGDYYNHGTVPRR
                     GLRIARMLGHITYLSDDVMGEKFGRKLRHGNVKYSFDVEFEMESYLRYQGDKFAGEFD
                     ANTYLRMTRALDYFDPALDYDGDLSKALSRAKAKFVVISFTTDWRFSPARSREIVQAL
                     LDNALPVKYAEVTSAHGHDAFLMPDPYYHDIMRAYLEQIKV"
     gene            complement(7454..8251)
                     /locus_tag="VNZ84_04300"
     CDS             complement(7454..8251)
                     /locus_tag="VNZ84_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HWU66208.1"
                     /translation="MQSIPYIKAVLFDLDGVLYIGKQLIPGALSAVAQLRKAGIAVRF
                     VTNTSTLSLDSLQQKLNDLGFNTVPEEIMSAPQATIQYLKKQANPVCKLLLADDVKKD
                     FACFDQSATAANYVVIGDIGDQWSYELLNEVFHCLVNGAQLIAIHKNRFWQTETGLQM
                     DIGAFVTGLEYASNTQAMLMGKPSRHFFNQVVDTLRLKPSDIVMVGDDIDADVGGAQD
                     AGLHGILVKTGKYRETYTRLSAVEPDAIIQSVADLPALLGCLSIPAH"
     gene            complement(8309..9166)
                     /gene="prmC"
                     /locus_tag="VNZ84_04305"
     CDS             complement(8309..9166)
                     /gene="prmC"
                     /locus_tag="VNZ84_04305"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWU66209.1"
                     /translation="MAQTVQQLLQTARQQLAQLVPQDEASIEARLLLMHVLEVNHAWL
                     LTHATDTPAEIHAQTFQRHVQRRLQGEPVAYILGYREFFGLKLRVTPDTLIPRPDTEI
                     LVEIALAKVTPGMQVLDLGTGSGAIALAIAQQAPRVLVTAVDASQGALSVAESNLKHL
                     QLDNVSLCLSHWFDALPQRVFDLIVSNPPYIEDSDPHLNQGDLRFEPPSALASGADGL
                     QDIRHIAAHATSHLNPGGWLMLEHGYNQADAVQELLNTHGFRAVETIKDLGGQPRVTI
                     GQWPGRKPV"
     gene            complement(9166..10014)
                     /locus_tag="VNZ84_04310"
     CDS             complement(9166..10014)
                     /locus_tag="VNZ84_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HWU66210.1"
                     /translation="MTQFRLPAAPRSIQKDNPIKFLLDREAVDCLAHNIGLVHASFDA
                     EAFKQLALTNIDPLGFMDRSAHIAAALKATLPAKFSDATEILLNTLTPPNVHTERLGL
                     SVFFYLPHTRFVAEYGRDAQHNDGEDPFDIAMRAQYELTRRFTAEFSIRPFLIHDFER
                     TLNQLTLWLNDPDPHVRRLCSEGCRSRLPWGARIPQLIADPTPVLPILEALKNDSSLY
                     VRRSVANHLGDIAKDHPELAFDICERWLTNADKDLKWVIRHAVRHPAKQGNATAIAIR
                     KASGGK"
     gene            complement(10032..10451)
                     /locus_tag="VNZ84_04315"
     CDS             complement(10032..10451)
                     /locus_tag="VNZ84_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWU66211.1"
                     /translation="MTAAAPISKHLGSCLCGAVRYEVHGELGALVYCHCQRCRKASGS
                     AFNAVIPISGANFTITQGEAALNTYRSDAGFCRTFCGHCGSPIMAYRESDPDALRLRV
                     GTLDTPVAAQVSAHIFTDSKAEWHEIHDKAPQYPERP"
     gene            complement(10458..11540)
                     /gene="prfA"
                     /locus_tag="VNZ84_04320"
     CDS             complement(10458..11540)
                     /gene="prfA"
                     /locus_tag="VNZ84_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWU66212.1"
                     /translation="MKPSMLQKLANLSERLEELNRLLSSEGVTDNMDNYRKIMQEHTE
                     ITPIVEQYHIYSQTESDLKEAHNMLSDPEMKEFAQEEIESGKKRLEEVALTLQKLLLP
                     KDPNDDKNIFLEIRAGTGGDESGLFAGDLFRMYSRFADRQGWKVEIVSANEGEVGGYK
                     EIIAKINGYGAYSKLKFESGGHRVQRVPDTETQGRIHTSACTVAILPEVEEVGDVEIN
                     PADIRIDTFRASGAGGQHINKTDSAVRITHAPTGIVVECQDGRSQHANKAQAMQVLAA
                     RIKAKQVDEQQSKIASERRNLIGSGDRSERIRTYNYPQGRITDHRINLTLYKIEAITE
                     GDMDELINALATEYQADLLASLGEDA"
BASE COUNT         2716 a         2912 c         3000 g         2927 t
ORIGIN      
        1 ggtaaacaat gcgagttgcg tcgcagtgta agcaggattg gtttccctgg ccatataggc
       61 cacataggca gcggtgccca ggccaacccc aaaggcttca aagccaatca caattgctag
      121 catgccacgt tcagttgcgc caatgtctgt gaaaggacca ctcgttgcca accatgcgaa
      181 gccgagaatt gataaggctt gcaccacgcc aaatacccat aaaccacgat taatgccgag
      241 ttttagcatc cacaagccac caataaacgc acctgcaatt tgcatgacga ggccgctacc
      301 tttggcaatc gcaccgatgt ctgttttgct gaagcccata tcaatataaa acggggtagc
      361 cagggcagtg gccatgctat ctcccaattt atacagcaag ataaacagca atacccacag
      421 cgcatgttgc cagccgcttc gctgcataaa ttccttgaaa ggctcaacca cagatgccaa
      481 caaagtcttg gggacgctag taatgacagg ttcttttgcc cagagcgtga agcctatgcc
      541 aggcagcata aataaaccca cgatcagaaa cacctgtgac caggccatac gatcggccag
      601 tatgagcgca agtgagccag gtaccaggcc ggcgatacgg taagcattca catacaggct
      661 attgcctaat cccagctctt catcgctgag cgtttcacgg cggtatgcat caatgacaat
      721 atcctggctg gcagagaata aggcgaccaa tgtcgcgagt agcgcaacca tggcgatgtc
      781 cagttgtggt tggcacatgc ccaggccgac gatgctggcg aataaagcca gctgggtcgc
      841 taaaatccag ctacgccgtc tgcctagcgc ttgtagcaag ctatagcggt ccatgagggg
      901 tgcccataga aacttccagg tgtatggcaa ttgcataaaa gcaaacgcgg cgatggtttt
      961 taagttgagg ccttcgctgc gtagccaggc gggcaataat tgcaacagga gatataaggg
     1021 taagccagag ctgaagccta atgccacaca catcagcatg cggcgggtga acagggtttg
     1081 tgtgagtgtc atgcctgcat tgtaaacgct tttacaatat cgctaatggg catttctatc
     1141 aattcaactg tggttgcaat acagcgttgc ccataaaaaa tttctccttg tctgactgag
     1201 taatttggat ttatagaaac accctgatat cagcacaaaa aaaagccaac cctttcgggt
     1261 tggccacact cggagatcaa tggtattaca gggtcttaca aacttaacac ccactggaca
     1321 agggttttaa tatcctcatc tttaacttgt gggctgttag gcggcatggg catatcaccc
     1381 caaacacctg cgccgccggc ttttactttc tggatcaact tggcttcagc agacttgtcg
     1441 cctttgtatt tggcagccac ttctttgtaa gctgggccta gtactttttt gtctacgcta
     1501 tggcagagca ggcaaccact tttctgtgcc agtgcttcag caccactacc cgcaatggct
     1561 gaggccgtca ccgctagcaa tgccgtcgcc agcaaggttt tgttgaataa ttgcatcagt
     1621 tttccttcct tataagctga atacgttcat ggcaccgcca cctggcgccg cgttgtattt
     1681 ggtcagctct ttatagccac cggccgcacc caagccatcg gtatcattag tcaggccaag
     1741 gttcatggcg acacccgccc agccaccaat gccagacaat acgcctacgt attgcttgcc
     1801 tttatggccg taagtaatca tgttgccaac cacgccagaa cccacctgaa atttccacag
     1861 ctctttaccg gttttggcat ccagtgcttt tacccagcgg tccaatgtgc cgtaaaacac
     1921 tagatcagac gcagtcacca atgcaccacc ccaggcagag aatttctctt tgttgtacca
     1981 ctgggctttg cctttgaggc catcccaggc cataaagcca cccatcacac catcagggcc
     2041 tgcaaacatg gtcagggttg cacctaccca tggttggcct gcgacgtatt tggactctac
     2101 tggctcatag gtcatacata cgtggttcag tggcacgtag aatgttttgt tttttggaga
     2161 gtatgctgct ggctgctcat ctttcacacc tagtgcagat ggacagacgc ctttagcgtt
     2221 gtagtcctgg tgagtggagt atttcgggtc tttgaccgga atgcctgttt tcaggtcaat
     2281 cttggttgcc cagttaacga atggatgcat tttttctgcg accagcaagg tgccattggt
     2341 gcggtcgaaa gtatagccaa agccattacg gtcaaaatgt gaggccagtt gcttgccgtt
     2401 ggcttcccac aggatgattt cgttcatgcc gtcgtaatcc cactcgtcat gcggtgtcat
     2461 ctggtaaccc cagcgtgcca taccggtatc cagatcgcgg gcaaaaatcg tcatcgacca
     2521 cttattatct ccaggacgta cgtccgggtt ccaggtacct gggttgccgg taccgtagta
     2581 catcaggttc agtttagggt cgtatgagta ccaaccccag gttgggccac caccattttg
     2641 ccagccatcc ttttgttgtt gcggtttcag ccaggtgcgt acacccaggt tggcttcagg
     2701 aaatttcagc tgggctttgt ccaggctctt gaaagagcca cccattttgt tgccgccgtt
     2761 gacatcttca tagaccgaca gtgcgctgta gtgcggattt tccttgttga aatccgcgcc
     2821 tatcagtact tcgctgtcag agccagtgct gtaagctttc caggccaggc taccatcctt
     2881 gatgttgtag gcgctgatat ggcaacgcac accgaattcg ccacctgagc agccggtcac
     2941 gactttgtct ttaaatacat gcggtacgtt ggtatttgca gaacccattt tcgggtcgtt
     3001 tactttgaca tcccagagtt ttgcgcccgt ttttgcatcc agtgcaacca atacaccatt
     3061 gttttgttgc aggaaaatct tgccgtcgcc gtaaccaagg cctttattca cgttgtcaca
     3121 gcacaataca gattgcacat caggatcttg tttcgggaaa taagaccaag cgattttctg
     3181 gttgtcatcc aagtctagcg catacacgtt gtttgggaat gcagtgtgga tgtacatggt
     3241 attgccaatg accagtggcg caccttcgtg accacggtta acaccagtgg caaaggacca
     3301 agcgagtttc agttgtttga cgttgctttt tttgatttgc gacaggcggc tgtaaccctg
     3361 gttggcgtaa tctttacgcg ccatggccca ttggttgtcg tctttaatca tggcgtccag
     3421 ttcggcattt gcccatgcgc tggtgctgat gcttcctaac agcattgcgc cgatggcaag
     3481 cttgatacgt tgttgcataa ataacctcca aaatggttgc ggtgaagctg gtatgagtgg
     3541 tgttgcaaac ggtcaacaaa ggcttctttt gtgactgcca gcctgtgatt gcaacgtgtg
     3601 tgccaactgg tgacgttatt ttggaaattg taaaaaatga attaaaatca aaaggttaat
     3661 ttcgtatgag aacattctgg ataacccgtg caatggcggg aactgttcac gactgtgtca
     3721 tactggtaca gttgttcaac gctatgtaac aggtgttcag ttttaaatgc ccaataaata
     3781 ttccccgtct accgcccaaa cgtcacagcc tggtctgcga gatgatcaac gtgcgattgc
     3841 tgaggccatt gcacgctcat ggcggcgatg catggttaaa ggtgtggatg aacaggcacc
     3901 ggttgaagac cagattatta cggcgcaaga gctgaatcag cgccttgaac agaaccgctt
     3961 gctgctcaca catgcggagc ctgagatgct cacgttgagc gagcagattg cccatacgcg
     4021 cagtctggtg attttgacgg atagtgaagg gattatcctg cgcaccatgg gcgagggcgt
     4081 ggaagaaaat gagatccgtg cgtctttatt acccggcgcc tcctggagcg aagagcaccg
     4141 tggtaccaat gcgattggta cggcgctggt ggagcgtctg cccatcagcg tacaaggtac
     4201 cgaacacttt atggcttacc accattcgtt gagctgttct gccgtgccaa tttttgcggc
     4261 caataaccac ttggtggcca cgctggatgt ctccaacgat ttaaatgcgc cgcagcagca
     4321 tacgctggca ctggtgaaaa tggccgcaca aatggtagaa aaccgcctgt tccatgccac
     4381 tgctgaaggt gaaattggcg tgcatttcca tgtgcgacca gaattcatag gtactttgtg
     4441 ggaaggcgtg gccctgtttg atacgcaagg ccaattgcag gcaattaacc gcagtggaca
     4501 attccagttt ggtttaccgt tagaccagga cgtgttgtct gcacgccgta ttcagttcga
     4561 ggatattttt gacgaatcct ggaccgtatt caagcaacgt ggattgtctg ccagcatgtt
     4621 atttcccctg cggctgcgca atggtgcccg tttgttcgcg cgtgcttctg caacgcaggc
     4681 tcctcttatt aacagcaagc agccgattcc atcaattaca cgcgagtctg ctgccagcct
     4741 ggagttactt gatagcggcg acccgcaatt gaagctggct atcagccagg tgaagcaggt
     4801 gctcaatcgt gacattccca tcctgatttt gggtgaaacc ggtgcaggca aagaattgtt
     4861 ttcccgtgcc attcacgatg ccagtgcgcg tcgtgacaag ccatttattg cagtgaactg
     4921 tgctgccctg cctgaggggt taattgaggc agagttgttt ggctatgaag aaggtgctta
     4981 taccggcgcc aagcgtaaag gcaatatcgg caagatacag caggccgagg gtgggaccct
     5041 gttcctggat gaaattgggg atatgccact gtcattacaa gcgcgcttgt tacgggtatt
     5101 gcaagaccgc agtgtgaccc cgctaggttc aagcaagtcg attccggtga attttatgct
     5161 ggtgagtgcc actaaccaga agctcaagga taaagtggcg gcaggcgagt tccgtagtga
     5221 tttgtattac cgcattaatg gcttggccgt gcaattgcct gcattgcgcc agcgacagga
     5281 tatggcacgc ctgattcaga tgattttgca aattgagcaa gcgccgcaag tgaccttgag
     5341 tgaagaggtc atggcgttat ttgctaccca tccgtggcca ggcaatgtgc gtcaattaca
     5401 taatgtgctg cggacggcag tcgcgctggc cgagggtggc gttattcaaa ggcagcattt
     5461 aacacatgat ttcctggatg aaatggcggt atgttcagag cttgtggcaa cggctgttgt
     5521 cccaacttct gttacaggaa acctgaaagc gcaatccgaa gctgcaatac gccaggccat
     5581 gcttcagcat agcggcaata tttcggccgt ggctcgccag ctcggcatca gcagaaatac
     5641 actttaccgc cggctaaaag agttggattt agcttaggcc gcttttttca agcggtacat
     5701 ggccaggttg cccatcagat tgggcaatat atgcacatct tggccaccag taatgacttt
     5761 gcgttctatc acctgaattt ggtgctggac acaaaactgg tcaaaatcat taatcgtgca
     5821 caagtgcaca ttaggcgtgt cataccattg gtatggcaag cttttggaga ccggcatggt
     5881 gccaaaggtg atctgcatgc ggttgcgcca gtaaccaaag ttaggaaaag taacgattgc
     5941 ttcgcgaccc actcgcagca tctcctgcac aatgctttca gtgttgtgca tggcttgcaa
     6001 ggtttgtgac agaatcacag tgtcaaatga ctggtcttca aagcccgaca aaccttcttc
     6061 caggttcatc tgaatcacat ccacaccatt cttaagcgcc gccaaccagt tggcgtcatc
     6121 tttttcaatg ccgtagccag tggtttccag tgtttcgtgc aaatgcgtca gcaaggtgcc
     6181 atcgccacaa cccaagtcca gcacttttgc ttttgtgctg acccagtttg taattaatgc
     6241 aaaatctggg cgaatatttg tattttttac actcatactt taatttgctc cagataagcc
     6301 cgcatgatgt cgtgataata agggtcaggc atcaaaaatg catcatggcc atgtgccgaa
     6361 gtcacttcgg catatttcac tggtaaggca ttgtctagca acgcctgcac gatttcacgc
     6421 gaacgcgcag gggagaaacg ccagtcggtc gtgaacgaaa tgacgacaaa cttggccttg
     6481 gcacggctaa gcgctttgct caaatcgccg tcataatcca gcgccgggtc aaagtaatcc
     6541 agcgcacgcg tcatgcgcaa ataggtgttg gcatcaaact cacctgcaaa cttgtcgccc
     6601 tggtaacgta aataagattc catttcgaac tcaacgtcaa agctgtattt cacattgcca
     6661 tgacgcagtt tgcggccaaa tttttcaccc atgacatcgt cagataaata ggtgatatgg
     6721 cctaacatgc gcgcaatgcg tagtccgcga cgtggtaccg taccgtgatt gtagtaatca
     6781 ccgtcaaaaa actccgggtc ggtaatgatg gcttggcgcg cgacctcatt gaaggccatg
     6841 ttttgtgcag tcaggttcgg ggccgaggca atcacaaacg catgccgcac gcgctcaggg
     6901 taagcaatgt tccactgcag ggcctgcatg ccacccaggc tgccaccaat gacggccgcc
     6961 agttggtcta tacccagata gtcgagtaaa cgtgcttgtg actccaccca gtcttcaacg
     7021 gtgaccacgg ggaatgttgc actgtaaggc cggtcagtca gcgggttcac actggacgga
     7081 ccactgctgc cgtgacagcc acccaggttg ttgaggccaa tgacaaagaa tttattggta
     7141 tccagcggtt tgccggggcc aatcaggttg tcccaccagc caggatattt atcttcaggc
     7201 gaatagcggc ccgcaacatg atggttgccg gacaaggcat ggcaaatcag cacggcattg
     7261 gatttatccg cgttaagctc gccataggtt tcataagtaa gatggtattg cggcagcact
     7321 gcaccacttt tcagtgtcag cggctgggta aaatgcgcaa cttgcgcttt aacgatacca
     7381 acagaattcg attcagacat gccgcgatta tactatgcgg catgccgaaa cggcgagaca
     7441 ggctgtgtgg tgattaatgg gccggaatgg acaaacaacc cagcaatgcg ggaaggtccg
     7501 cgacggattg gataatcgca tccggctcaa ctgctgaaag ccgggtatag gtttcgcggt
     7561 atttgccagt tttgaccaga atgccatgta gtccggcatc ctgcgcgcca cccacatcgg
     7621 catcaatatc gtcacccacc atcacaatgt ccgaaggctt caagcgtaag gtgtccacta
     7681 cctgattgaa aaaatggcgt gaaggtttgc ccatcagcat cgcctgggtg ttgctggcat
     7741 attccaggcc ggtgacaaaa gcgccgatat ccatttgcag gccagtttct gtttgccaga
     7801 aacggttttt atgaatagcg attagctgcg cgccattcac caggcaatga aatacctcat
     7861 tcagtagttc ataagaccac tggtcaccaa tatcaccaat caccacataa ttggctgcgg
     7921 ttgcagactg gtcaaaacag gcaaagtctt ttttaacgtc atctgccaat aagagtttac
     7981 agacggggtt ggcttgtttc tttagatact ggatggtcgc ttgtggtgca ctcatgattt
     8041 cctcaggcac tgtattgaag ccaaggtcat taagtttttg ctgcagtgag tctaaggaaa
     8101 gtgtgctggt gttagtgaca aaacgcactg caatgcccgc tttgcgcaac tgggccacag
     8161 cggacaatgc gccgggaatc aattgtttgc cgatgtagag cactccatcc aggtcaaata
     8221 aaacggcttt gatatagggt atcgattgca taaagtgtct ttccagagag tgatgcttga
     8281 gactaacgta tctttactca agcaaaaatc atactggttt acggccaggc cattgcccta
     8341 tcgttacccg gggctggccg cccaggtctt tgatggtttc gactgcgcga aagccatgtg
     8401 tgttcaataa ttcttgtacc gcgtcagctt ggttgtagcc atgttccagc atcagccagc
     8461 cgcctggatt caagtgactc gttgcgtgtg cagcaatatg acgaatgtct tgcaggccat
     8521 ctgcgcctga tgcgagcgca ctgggcggct caaagcgcaa gtcgccctgg ttcaaatgcg
     8581 gatcagaatc ctcaatataa ggcgggttgc tcacgatcag gtcaaataca cgttgcggca
     8641 gtgcatcaaa ccagtggcta agacacaggc tcacgttgtc caattgcagg tgctttaaat
     8701 tactttcggc aaccgataat gcgccttggg aagcatcgac ggcagtgact agcactcgcg
     8761 gggcctgctg ggcaattgcc agggcaatgg cgccactccc cgtgcccaag tccagtacct
     8821 gcatcccggg cgttactttt gccagcgcta tttcaaccaa tatttcggta tccggcctgg
     8881 ggatcagcgt gtctggtgta acgcgcaact tcaaaccaaa aaactcacgg taacctaaaa
     8941 tataggccac gggttcgcct tgtaaacgac gctgcacatg cctctgaaat gtttgcgcgt
     9001 gaatctccgc tggcgtatca gtggcatgcg tcagtagcca ggcatggttt acctctagta
     9061 catgcatcaa caacagtctg gcctctatgc tcgcctcatc ttgcggcacc aattgcgcca
     9121 gctgttgccg tgccgtttgt aataattgtt gtaccgtttg cgccattatt taccgccgga
     9181 ggctttgcgt atggctatcg cggtggcgtt tccctgtttt gccggatggc gcactgcatg
     9241 acggatcacc catttcaggt ctttatccgc attggtcagc cagcgctcac atatgtcaaa
     9301 ggcaagttct ggatggtctt tggcaatatc gcccaaatga ttggcgacac tacggcgcac
     9361 atacaagctt gagtcgtttt ttaatgcttc cagaatcggc aacacggggg ttggatcagc
     9421 aatcagttgc ggaatgcgtg cgccccatgg caagcgtgag cgacaacctt ccgaacacag
     9481 gcggcgcaca tgcgggtccg ggtcattgag ccataaggtc agttgattca gtgtgcgctc
     9541 aaaatcatga atcaaaaacg gacggattga aaactctgcg gtaaagcggc gcgttaattc
     9601 atactgtgca cgcatggcga tatcaaatgg atcttcgcca tcattgtgtt gcgcatcacg
     9661 gccatattcg gcgacaaagc gtgtgtgtgg taggtagaaa aatacactca gccccaggcg
     9721 ttcggtatgc acattgggtg gggtcagtgt attgagcagg atctcagtgg catctgagaa
     9781 tttggctggc aatgtcgctt ttaatgcggc cgcaatatgc gcggagcggt ccataaatcc
     9841 cagaggatca atgttggtca atgccaactg cttaaaggct tccgcatcaa agctagcgtg
     9901 caccaacccg atattgtgtg ccaggcaatc tacagcttcg cgatcaagta aaaacttgat
     9961 cgggttgtct ttctggatac tgcgtggggc ggcgggaagt ctgaattggg tcatctgaga
    10021 cggctccctt gctaagggcg ttccgggtat tgtggagctt tgtcgtgaat ctcgtgccat
    10081 tccgctttgg agtctgtaaa aatatgagca gagacttgcg cggcgacagg cgtgtccaag
    10141 gtgccgacgc gcaaccttaa cgcatccgga tcgctttccc ggtacgccat gattggggag
    10201 ccacagtgac cgcaaaacgt acggcagaaa ccggcgtcgc tgcggtaggt gttgagcgct
    10261 gcctcgccct gcgtgatggt gaaatttgcc ccggagatgg ggattacggc attaaatgca
    10321 gagccagagg ctttgcggca acgctgacag tggcaataca ccagcgcgcc aagttcgcca
    10381 tgcacttcgt agcgcacagc gccacacagg cagctgccca ggtgtttgga gataggggct
    10441 gcggctgtca tttagtgtta tgcgtcttcg ccgaggctgg ccagcaaatc tgcctggtac
    10501 tcggtggcca gtgcattaat cagttcatcc atatcaccct cagtgatcgc ttcaatttta
    10561 tacagcgtca ggttgatgcg gtgatcggta atgcggcctt gaggataatt gtaggtacgg
    10621 atacgttctg agcggtcgcc cgagccaatc aggttgcggc gctcggaggc gattttgctt
    10681 tgttgttcat cgacctgttt ggctttaatg cgcgcggcaa gcacttgcat ggcttgtgcc
    10741 ttgttggcgt gctggctgcg gccatcctgg cactcgacta cgataccggt tggtgcatgg
    10801 gtaatccgta cggcagagtc ggttttgtta atgtgctggc caccggcgcc cgaggcgcgg
    10861 aaggtatcaa tacggatatc ggccgggttg atttctacat cgcctacttc ttccacttcg
    10921 ggcaaaatcg cgacggtaca cgcggaggta tggatgcggc cttgcgtttc ggtgtcagga
    10981 acgcgttgta cacggtggcc accggactca aatttgagtt tggagtaagc gccataaccg
    11041 ttgattttag cgatgatttc cttgtagcca ccgacttcgc cttcattggc ggagacgatc
    11101 tcgactttcc agccttgacg gtcggcgaag cgggagtaca tgcggaataa atcaccagca
    11161 aacaggccgg attcgtcacc gccagtacca gcgcggattt cgaggaaaat gtttttatcg
    11221 tcgttggggt ctttagggag caacagcttt tgcaaagtca gtgcaacttc ttccaggcgt
    11281 tttttgccgc tctcaatctc ttcctgggca aactctttca tttccgggtc tgagagcatg
    11341 ttgtgcgctt ctttgagatc ggactcggtc tggctgtaga tatggtattg ctcgacaatc
    11401 ggtgtaattt ccgtatgttc ctgcatgatc ttacgatagt tatccatatt gtcagtcacg
    11461 ccttcgctac tcagcaggcg gttaagttct tcaaggcgct cgctcaagtt cgcgagtttt
    11521 tgcaacatac tgggtttcat aagttttttt accta
//
LOCUS       DATMSK010000127         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_315360,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000127 DATMSK010000000
VERSION     DATMSK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315360"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VNZ84_04325"
     CDS             complement(<1..577)
                     /locus_tag="VNZ84_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWU66213.1"
                     /translation="MHAALLASPLVHFTLPRYLANVCLWLAVMIIAWSPFSGGARLPT
                     LLLAIVGGGLLLTSLPALSRQLAVRRWTTVFLLLWLPMWLSLVHAFSVKATFAAIGWF
                     SLIYLAGIALVLLLQQSIYRRLFAASLTVVMSLWILDSAIQYLFGVDIFGIPKTPEGR
                     ITGMFSDLHKGILMLPMLPLIFYHWQPKRAWL"
     gene            complement(580..1704)
                     /locus_tag="VNZ84_04330"
     CDS             complement(580..1704)
                     /locus_tag="VNZ84_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWU66214.1"
                     /translation="MRKLTVLQVLPALQSGGVERGTLEIASHLVSQGHRSLVMSAGGR
                     MVPALEATGSTHFTCAIGRKSLLTFLLIPKLRKLWRQEQVDIVHVRSRFPAWVVFLAW
                     KGMPPAQRPHLVTTVHGYYRPGWYSRIMTCGERVIVISKTIQSYVQREYLVNAEKLQL
                     IFRGVDTTQYTPGYQPDSDWQKSWYAQYPQTRGKRLLVLPARISRWKGAEDFVDLLSI
                     VCRQHKDVHALLVGEVAQDKEAFAAHLQQKIQQADLAERVTLTGYRQDVKQIMAISSI
                     AYSLSLEPEAFGRTTVEALSLGVPVIGYQHGGVAEQLAALLPEGAVQVGNVAEAAMLT
                     LSWLEQPPAVMPNTRFSLQVMQQRTLEVYQQVVADCGQEP"
     gene            complement(1750..2721)
                     /locus_tag="VNZ84_04335"
     CDS             complement(1750..2721)
                     /locus_tag="VNZ84_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HWU66215.1"
                     /translation="MLSTNHSNNSLVVWRLLDGKPGHEHQSLGLVNAIRQRNPVHVVD
                     IPVTHPGLGWMYVLGGVWPDGQALPKPDMIIGAGHATHGHMLAAKRAYGGKTVCMMQP
                     SLPAEMFDVCLIPEHDQYWGFSSFIETRGVINHIQPTNADKKDNNDVLIMIGGPSKHF
                     HWDELGVVAQVYDLVRKHSEVHFILTTSRRTPASFVSAMQRVRLANLTIIPISDTDKD
                     WVQTTLAKVSTAWVTEDSVSMVYEALTAQVAVGLINLQAKGKSRVSRGIEKLIDRQFV
                     TRYDPYGVYRQYMRPVLGFNEASRCCEALLAYLNPASTRRRLVSRLS"
     gene            complement(2837..>3103)
                     /locus_tag="VNZ84_04340"
     CDS             complement(2837..>3103)
                     /locus_tag="VNZ84_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HWU66216.1"
                     /translation="TDMAAKFLPIVQKATANVQLADTYNKYAEMGSKFGVIKKEDANI
                     NQYVTQKALDGVYLMIAKEEAAIRQDPMGQASNLLKKVFGAVSK"
BASE COUNT          802 a          872 c          735 g          694 t
ORIGIN      
        1 ccagccatgc acgcttgggc tgccaatgat agaaaatcag tggcaacatc ggcaacatca
       61 atatcccctt gtgcaaatca ctaaacatgc cggtaatgcg tccttctggg gttttaggga
      121 tgccgaaaat atctacacca aacaggtatt gaatcgcact atccagtatc cacaaactca
      181 tgaccacagt gagactagcg gcaaacaatc ttctgtatat ggattgctgc aagagcaaca
      241 ccagggcaat accggccaga tagattaagc taaaccagcc gatggctgcg aacgtcgctt
      301 tgaccgaaaa tgcatgtacc aaggatagcc acatcggcag ccataacagc aaaaatactg
      361 tggtccagcg acgtactgca agttgcctac tcaaagcggg taaagaagtc agcaacaacc
      421 cgccaccaac aatggccagc aataatgttg gcaacctggc tccaccagaa aaaggactcc
      481 aggcaatgat catgactgcc aaccataggc aaacattggc caaatagcgc ggcagggtaa
      541 agtgcacaag tggagatgcc agcaaagcag cgtgcatgat taaggctcct gcccgcaatc
      601 agccactact tgctgataaa cttccagggt gcgctgctgc atgacctgca atgaaaaccg
      661 ggtattcggc attacggcag gcggctgctc aagccagctc aatgtcaaca tcgctgcttc
      721 agccacattc ccaacttgca ctgcgccttc aggcaataac gccgccaact gctcagccac
      781 gccaccatgc tgatagccga tcactggcac gcctagactc agcgcttcta cggtcgtgcg
      841 gccaaacgct tccggctcca atgaaagaga ataggcaata ctggagatcg ccatgatttg
      901 cttgacgtcc tggcggtatc cggtcagcgt gacacgctca gccaaatcag cctgttgaat
      961 tttttgctgc aagtgcgccg caaacgcctc tttatcctga gccacctcgc ccaccaataa
     1021 agcatgcaca tctttatgct ggcggcagac aatactcagc agatccacaa aatcttccgc
     1081 ccctttccag cgtgatatcc tggcgggcaa aaccagcaaa cgcttgccac gggtttgcgg
     1141 atactgtgca taccatgact tttgccaatc ggaatctggc tggtaacctg gcgtgtactg
     1201 tgtcgtgtcc acaccacgga aaatcaattg cagtttctct gcattgacca gatactcgcg
     1261 ctgcacatac gactggattg ttttagaaat cacaatcacg cgctcaccgc aggtcatgat
     1321 gcggctatac cagccgggac ggtaataccc atggacggtg gtcaccaaat gcggccgctg
     1381 cgcaggcggc atccctttcc aggccaagaa cacgacccag gccggaaaac gtgagcgcac
     1441 atggacgatg tcgacttgtt cctgccgcca tagcttgcgt aatttgggga tcaacaggaa
     1501 ggtcaatagc gacttgcgcc caatggcaca ggtaaagtgg gtgctgcctg tggcttccag
     1561 cgcgggcacc atacgtccac cagccgacat caccaaagac cgatggcctt gtgaaaccag
     1621 atgtgatgcg atctccaatg taccccgctc caccccaccc gattgcaagg cgggcaatac
     1681 ttgtaacacc gtcaatttac gcaaaacaga aactcaaccg ctcaacagga gactatcaaa
     1741 atgtgttcat taagatagtc gtgacaccag gcgacgtctt gtggatgcag gatttaaata
     1801 ggccagtaac gcttcgcagc aacggctcgc ttcattaaac cccaacaccg gccgcatata
     1861 ttgtcgatac acaccatacg ggtcatacct ggtgacaaac tggcggtcaa ttaacttttc
     1921 gattccacga ctgacacggc ttttaccttt agcctgcagg ttgatcagtc ccacggccac
     1981 ttgtgcagtg agtgcttcat acaccataga aaccgaatct tcggtcaccc aggccgtcga
     2041 caccttggcc agtgtggttt gcacccagtc tttatcagta tcgctaatcg ggataatggt
     2101 caggttggcc agcctgactc gctgcatggc ggagacaaag ctggcaggcg tgcgcctaga
     2161 ggtggttaag ataaaatgca cttcagaatg cttgcgcacc aggtcgtata cttgcgcaac
     2221 cacgcccaac tcgtcccagt gaaagtgttt actggggccg ccgatcataa tcaaaacgtc
     2281 attgttgtct tttttatctg cgttggtcgg ctggatatga ttaatgacgc cgcgtgtttc
     2341 gataaaactg gaaaagcccc agtattgatc atgctcggga atcaggcaaa catcgaacat
     2401 ttccgcgggc agactgggct gcatcatgca taccgtcttt ccgccgtaag cacgtttggc
     2461 cgccaacatg tggccgtgcg tcgcatggcc agccccaata atcatatccg gcttgggtaa
     2521 cgcttggcca tcaggccaga ccccacccaa tacatacatc cagcccaggc caggatgcgt
     2581 caccgggata tctaccacgt gaacgggatt ccgttgccgg atggcattga ccagtccaag
     2641 agactgatgt tcatgccctg gtttgccgtc taacagacgc caaacgacga gagagttgtt
     2701 tgaatgattc gtgcttaaca tttgcatcac ctgcatgcgt gcaggttaga tgcgtatcat
     2761 gacaaaatca ttaacctaaa aagaacaaaa tgtggcgcac cgcctccccc gctacctgtg
     2821 tggcggtgac catcctctat ttgctgaccg ccccaaacac ctttttaagc aggttactgg
     2881 cttgccccat tggatcctgg cgaatggcag cttcttcttt ggcaatcatt aaatagacgc
     2941 cgtcgagtgc tttctgggtg acgtactggt tgatattggc atcctctttt ttaatcacgc
     3001 caaatttgct ccccatttca gcgtacttat tataagtatc agccaactgc acattagccg
     3061 tggctttttg tacgatgggc agaaacttgg ccgccatatc tgt
//
LOCUS       DATMSK010000128         5439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_9509, whole
            genome shotgun sequence.
ACCESSION   DATMSK010000128 DATMSK010000000
VERSION     DATMSK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9509"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VNZ84_04345"
     CDS             <1..1158
                     /locus_tag="VNZ84_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWU66217.1"
                     /translation="RQPVTFVKGEGVWLTDTAGDRYLDALSGVAVNGLGHTHPKFVTA
                     LNAQIARLIHVSNIYQIAEQSALADKLAALSGMDRVFFCNSGCEANEAAIKLARLYGH
                     NKGIDHPEIIVMDQSFHGRTMATLSATGNRKVQAGFEPLVSGFLRVPFDDLEAVKTIA
                     SHKNNVVAVLVEPVQGEGGVHIPASLKAYLQGLREMCDANGWLLMLDEVQSGIARTGT
                     WFAFQHTGIVPDVMTLAKGLGSGVPIGACVARGVAAETFTPGKHGSTFGGNPLATAAG
                     LATLDIIAEEKLRENAETVGNYIREQFTASLKGATGVVNIRNAGMMIGIELDRPCGDL
                     VKQALAAKLLINVTSEKVIRLLPSLVMNKQEADELVSRLTPLILNFLKASA"
     gene            1181..2098
                     /gene="argF"
                     /locus_tag="VNZ84_04350"
     CDS             1181..2098
                     /gene="argF"
                     /locus_tag="VNZ84_04350"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWU66218.1"
                     /translation="MSIKHFLQFNDLARDEIEHIFARAKWIKSQFKDYKQYWPLVDRT
                     LVMLFEKASTRTRLSFEAGMQQLGGSAIYLNTRDSQLGRGEPVEDAAQVISRMSDIVM
                     IRTFEQSIIERFAQNSRVPVINGLTNEYHPCQILADIFTFIEHRGSIKGKTVAWIGDS
                     NNVCNTWLQAAEVLDFNVHVSTPPGYEVEPERANLYGTSHFESFADPMEAARGADLVT
                     TDVWTSMGFEAENEERRKDFADWQVDEDMMKVAKADAVFMHCLPAHRGEEVSAEVIDG
                     KQSVVWDEAENRLHTQKALMEYLLLGKVA"
     gene            2137..3360
                     /locus_tag="VNZ84_04355"
     CDS             2137..3360
                     /locus_tag="VNZ84_04355"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWU66219.1"
                     /translation="MSDIKKAVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEE
                     LEPAREKAKKFGVSEIYIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIAKR
                     LIEIANETKADAISHGATGKGNDQVRFELGAYALNPNIQIIAPWREWDLLSREKLLAY
                     AEKHGIPVEMKHKQGGSPYSMDANLLHISYEGRHLEDPSAEAEEDMWRWTVSPEKAPD
                     APEYLDIEFKNGDIVALNGKALKAHEILAELNRIGGKHGVGRLDLVENRYVGMKSRGC
                     YETPGGTIILKAHRAIESITLDREVAHLKDDLMPRYASLIYNGYWWSPERVALQTLID
                     HTQATVNGWVRVKLYKGNVIVVGRDSKTDSLFDSTIATFEDDKGAYDQKDAGGFIKLN
                     ALRMRIAANLHNRKK"
     gene            3365..4972
                     /gene="cimA"
                     /locus_tag="VNZ84_04360"
     CDS             3365..4972
                     /gene="cimA"
                     /locus_tag="VNZ84_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HWU66220.1"
                     /translation="MASRRVIAYDSTLRDGAQAQGVSFSVEDKLKVVQRLDQLGIGYI
                     EAGNPGSNPKDLEFFARVGELHLQHAKIIAFGSTRRIGIAAAEDRNLQSLLSANTQAV
                     AIFGKSWDYQVTDILRTTLQENLAMISDTISYLKSQGKEVVFDAEHFYDGYKHNADYA
                     IQTLQSAVEAGADVLALCDTNGGTFPNEVFAITEAVVKRFPSVQIGIHCHNDCEMAVA
                     NSVAAVQAGAIQVQGTINGIGERCGNANLVSIIPNVQLKLGLACISDVQLSELTAAAR
                     FVSEVANVAFNDKAPYVGQDAFAHKGGMHIDAVNKNPKSYEHINPGAVGNERQILVSE
                     VAGRGAILRKLQQIDPSLTKESAAAVDILEQLKALEHEGYQFENAESSFDLLMQKLLG
                     KFKPFFSLKHYHVDIDEPSVDGQNSTATIQICVNDQESSATAYGDGPVNALDKAMRAA
                     LEQFYPAIKDIKLVDYKVRVLDSNLATAAKVRVLIESSDQQTSWTTIGVSTDIIEASW
                     LALKDAIEFKLLMLQQQQSNPALTAVL"
     gene            complement(4981..>5439)
                     /gene="pagP"
                     /locus_tag="VNZ84_04365"
     CDS             complement(4981..>5439)
                     /gene="pagP"
                     /locus_tag="VNZ84_04365"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HWU66221.1"
                     /translation="LKNHLVETWQKSDQLDLYVPLVTYHNRSMYSKEKIDSFNERPWG
                     LGIGKSYRDANGNWDGFYVMSFKDSHNDWEPIAGYGHVKNLVGKADSFNAGVGVTAGV
                     TARSDYQYKPLPVLLPIAAVGYDAVTVNATYVPGGKGNGNVLFMWSTVAF"
BASE COUNT         1390 a         1215 c         1447 g         1387 t
ORIGIN      
        1 aggcagcctg tgacctttgt caaaggcgaa ggcgtctggt tgacagacac tgccggtgat
       61 cgctatctgg atgcgctgtc cggcgtggcc gtcaatggcc tggggcacac ccatcctaaa
      121 tttgtgacag ccttgaatgc gcaaatcgcc aggttgattc atgtctccaa tatttaccaa
      181 attgccgagc aatctgcgct ggccgataag cttgccgcgc tttccgggat ggatcgtgtc
      241 tttttctgta actccgggtg tgaagccaat gaggcggcta tcaagctggc acgtttgtat
      301 gggcacaaca agggcataga ccatcctgaa attattgtga tggatcaaag cttccatggc
      361 cggaccatgg caaccttgtc tgccactggc aaccgcaagg tacaggcggg ttttgaacca
      421 ctggtgagtg gtttcctgcg tgtgccattt gatgatcttg aagctgttaa aacaatcgcc
      481 tcgcacaaaa ataacgttgt tgcggtgctg gtcgagccgg tgcaaggtga gggtggggtg
      541 catatcccgg ccagcctcaa ggcttatctg caaggtttac gcgaaatgtg tgatgccaac
      601 ggctggttgc tgatgttgga cgaagtgcag tcgggtattg ccagaaccgg cacctggttt
      661 gcattccagc atacgggcat cgtgccagat gtgatgacac tggccaaagg cctgggttca
      721 ggtgtgccta ttggcgcctg cgtggcgcgt ggagtggctg ctgaaacatt cacccctggc
      781 aagcatggct ctacgttcgg tggtaatcct ctagcaaccg cggccggctt ggctaccttg
      841 gatattattg ccgaagaaaa gctgcgtgaa aatgcggaaa cagtgggcaa ttacatcaga
      901 gagcaattta ctgcatcgct caaaggcgca accggggttg tgaatatacg caatgccggc
      961 atgatgatag gaattgagct ggacagacct tgtggtgacc tggtgaaaca ggcactggct
     1021 gccaaactgt tgattaacgt gacgtcggaa aaggtgattc gtttgctgcc atccctggtg
     1081 atgaacaagc aggaagcgga tgagctggtt tccagactga caccactgat attgaatttt
     1141 ttaaaggcaa gcgcttgatc attcaagtgc ccaacacatt atgagtatca aacacttttt
     1201 gcagtttaac gacctggccc gtgacgagat tgagcatatt ttcgcccgtg ccaaatggat
     1261 taaatcacag tttaaagact acaaacaata ctggccactg gtcgatcgca ccctggtgat
     1321 gctttttgaa aaagccagta cgcgtacgcg tctgtcattt gaagctggta tgcagcaatt
     1381 aggcggttcg gccatttacc tgaatacacg tgactcgcaa ctgggtcgcg gtgaaccggt
     1441 agaagatgcg gcgcaggtga tttcgcgtat gagtgacatc gtcatgatcc gtacctttga
     1501 gcagagcatt attgagcgtt ttgcgcagaa ttcacgtgtg ccggtaatca atggcttgac
     1561 caacgaatac catccatgcc agattctggc agatattttt acttttatcg aacaccgagg
     1621 ttcgataaaa ggtaaaactg tggcctggat tggtgacagt aataatgtct gcaacacttg
     1681 gttacaggcg gcagaagtac tggattttaa tgtgcatgtg tctacgccac cgggctacga
     1741 agtcgagccg gaacgtgcca acctgtatgg cacctctcac tttgagtcgt tcgctgatcc
     1801 gatggaagcg gcgcgtggtg ccgatttagt cactaccgat gtctggactt ccatgggctt
     1861 tgaagcggag aacgaagagc gccgaaaaga ctttgcggat tggcaagtcg atgaagacat
     1921 gatgaaagtg gctaaggccg atgcggtatt tatgcattgc ctgccagcgc accgcggtga
     1981 ggaagtgtct gctgaagtga ttgatggcaa gcaaagcgtg gtgtgggatg aggcagagaa
     2041 cagattgcac acgcagaagg cacttatgga gtatttgttg ttaggaaaag tggcttgatt
     2101 tcacaagcca gatgaattca gtttgggata atttagatga gcgatattaa aaaagcggtg
     2161 ttggcttatt ccggtggtct ggacacctcc gtgattttga agtggttaca agatacctac
     2221 caatgtgagg tggtcacgtt tacggctgat ttgggtcaag gcgaagagct tgagccggca
     2281 cgtgaaaagg ccaagaaatt cggcgtgagc gagatttaca ttgatgacct gcgcgaggag
     2341 tttgtgcgtg actttgtatt cccaatgttc cgcgccaata ctgtctatga aggtgagtac
     2401 ctcttaggca cctctatagc tcgtccattg attgccaaac gtctgattga aatcgctaac
     2461 gaaaccaaag ccgatgccat ttcacacggt gcgactggga aaggtaatga ccaggtgcgt
     2521 tttgaactgg gtgcttacgc cctcaaccca aatatccaaa tcattgcgcc atggcgtgag
     2581 tgggatctgc tgtcccgtga aaagctgctg gcgtatgccg aaaagcacgg tatcccggtt
     2641 gaaatgaagc acaaacaagg cggtagccca tattccatgg atgccaactt gttgcatatt
     2701 tcttacgaag gtcgtcatct ggaagaccct tctgctgagg ctgaggaaga tatgtggcgc
     2761 tggaccgtgt ctccagaaaa agcaccggat gcgcctgagt acctggatat tgagttcaag
     2821 aatggcgata ttgttgccct gaatggtaag gctttaaaag cgcatgaaat tctggccgag
     2881 ttaaaccgta ttggtggtaa acacggcgtt ggccgccttg atttggtaga gaaccgttat
     2941 gtcggcatga aatcccgcgg ttgctacgaa acccccggtg gcaccattat cctcaaagcg
     3001 caccgtgcga ttgagagtat cactttagac cgcgaagtgg cacatttgaa agacgatttg
     3061 atgccacgct acgccagcct gatttacaac ggttactggt ggagcccgga gcgtgttgcg
     3121 ttgcaaacgc tgatcgacca tacccaggcc accgtgaatg gctgggtgcg cgtcaaactc
     3181 tacaagggca atgtgattgt cgtgggccgt gacagcaaga ctgattcact gtttgactcc
     3241 acaatcgcta cgtttgaaga cgacaagggc gcttacgacc aaaaagatgc aggtggcttt
     3301 atcaagctga atgctctgcg tatgcgtatt gcggccaatc tgcacaaccg taaaaaataa
     3361 ctttatggcc agtcgacgcg tcattgccta tgactccacc ttgcgcgacg gtgcacaagc
     3421 gcaaggggtt tcattttcag tcgaggacaa actcaaggta gtccagcgcc tggatcaatt
     3481 gggcataggc tatattgaag caggcaatcc aggttctaat ccaaaggatt tggagttttt
     3541 tgcacgcgtt ggtgagttgc atttacagca tgccaaaatt attgcgtttg gtagtacccg
     3601 tcgtatcggt attgcagcag ctgaggatag aaacctgcag tcactgctct ctgccaatac
     3661 tcaggctgtc gctatctttg gcaaaagctg ggattaccag gtaaccgata tcctgcggac
     3721 taccttgcag gaaaacctgg caatgatcag cgatacaatt tcatacttga agtcgcaggg
     3781 taaagaagtc gtattcgatg ctgagcattt ttatgatggc tacaagcaca atgcggacta
     3841 tgcgatacaa accttgcagt ctgcagtaga agctggtgct gatgtgctgg cattatgtga
     3901 taccaatggc ggtactttcc cgaacgaagt gtttgctatc accgaggcag tggttaagcg
     3961 tttcccatct gtgcaaattg gtattcattg ccacaatgat tgcgaaatgg ctgtggcaaa
     4021 ctctgtggct gcagtgcagg cgggcgcgat tcaggtacaa ggcactatca acggcatcgg
     4081 tgagcgttgc ggcaatgcaa acttggtttc gatcattccg aatgtgcagt tgaagttggg
     4141 attggcatgc atttcagatg tgcagttatc cgaactcacg gctgcggccc gttttgtcag
     4201 tgaagtggct aatgttgcat tcaatgataa ggccccttat gtaggccagg atgcatttgc
     4261 acacaaaggt ggcatgcata ttgatgcggt caataaaaat cctaaatctt acgaacatat
     4321 caatccgggt gccgttggta atgagcggca gattctggtg tctgaagtgg ctggtcgcgg
     4381 agctattttg cggaaattac agcaaattga tcctagcctg accaaagagt cagctgctgc
     4441 agtggatata ctggagcaat taaaagcgtt agagcatgag ggttatcagt ttgaaaatgc
     4501 tgaaagctct tttgatctac taatgcaaaa gttactcggg aaatttaagc ctttctttag
     4561 cctcaaacac taccacgtcg atattgatga gccctccgta gatgggcaga attcaacagc
     4621 cacgatccag atttgtgtca atgatcagga atcgagtgca actgcgtatg gcgatggccc
     4681 agtgaatgca ttggataaag cgatgcgtgc agcattggag caattttatc cggccatcaa
     4741 agatattaag ctggttgatt acaaggtcag ggtgctggat tctaatttag ctacagcagc
     4801 taaagtcagg gtcttgattg agtcttcaga ccagcaaaca agctggacca ccatcggtgt
     4861 ttcaacagat attattgaag cctcctggct ggcattgaaa gatgcaatcg aattcaaact
     4921 gctcatgctg caacaacagc aatcaaatcc agcactcact gcagtgttgt agtcataaga
     4981 tcaaaaagca actgttgacc acataaatag cacgttgcca tttcccttgc cacctggaac
     5041 ataggtggca ttgacagtga ctgcatcata acctactgcc gcaatcggta gtaaaacggg
     5101 taaaggcttg tattgatagt cacttcttgc ggtgactcct gccgtgactc ccacgccagc
     5161 attaaagctg tctgccttgc caaccaaatt cttgacatgc ccataaccgg cgataggctc
     5221 ccagtcgtta tgtgaatctt taaatgacat gacatagaaa ccatcccagt tcccgttggc
     5281 atcacggtat gatttgccaa tacccaaacc ccatggccgc tcgttaaagg agtcaatttt
     5341 ctctttggag tacatgcttc tgttgtgata ggttactaat ggcacgtata agtctaattg
     5401 atctgatttt tgccatgttt caaccaaatg gtttttgag
//
LOCUS       DATMSK010000129        13196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_291722,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000129 DATMSK010000000
VERSION     DATMSK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291722"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1880)
                     /locus_tag="VNZ84_04370"
     CDS             complement(<1..1880)
                     /locus_tag="VNZ84_04370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWU66222.1"
                     /translation="MSAPSITTASSVSHAGITHSGPLSLEEQTQLLNLQRAILKSIAR
                     GADHLEVINQTCLLAESLVPNSFSSVMLMDEKYVHLNVYVAPSLTPEQIQKINGLHPS
                     PGGGSCGNVIYQQAVQYVSNTLTDERWSEIRHLVDDLNILACWSIPIFSAENKVIGTF
                     ALSSYEHREPTQFHRMLLEIGSSIIGIVLNRNRYQESLRQYEKVFEGSEQGIMVTDAK
                     HVILSVNPAFTRMLGFTLDDVVGKTPKILSSGLHDSSFYRNLWESVNDFGHWRGEIWN
                     RRKNGEILPEWLSISEVKNESGEVSHYVGTFSDLSDLKSAQKEIQYLASHDALTGLPD
                     ITHFRDRLENAISIAAAANQKIALLALNLDNFKFLNDSLGYASGDKLLKLFAERLKGC
                     LHETDTICRHGGDEFILLLNNMHDDDSINYMVDMLLTEVSSPFAFAGSNISTSCSIGV
                     AVYPTDGNDFEKLFGRARKAMSHAKEEGRNTARFFTEKLNTDSLHYLNIAYGLREALA
                     HHQFELHYQPQISLKNAQVIGAEALIRWHHPQNGMQPPAQFISIAEQTGLIVEMGEWI
                     IEEACRQAMEWQKNGLPPLSMAVNVSAVQFRRGNLAKVIVKALEKTGLDPQLLELELT
                     ES"
     gene            complement(1958..3166)
                     /locus_tag="VNZ84_04375"
     CDS             complement(1958..3166)
                     /locus_tag="VNZ84_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWU66223.1"
                     /translation="MPLLTTLLILIVTARLMGQVFSRFNQPAIIGEMLAGVVLGPSIF
                     NLISPTPALSGLAEFAVFLIVLSAGLEMNFKEVVDSMRGKGIVIALLGFLLPLTGGIL
                     VGIGFDLDVNRTIFLGLCVSITALPVTVSILQSFKLLDSNIARYSVATAIFNDVAALL
                     VLGVILNLPEQKSYQAVGSAIFSASWKLIMLILLIVSVSLAIKKVTEKGVRIDQWSEK
                     LVEFIGNEALFGLLLLLVLVFGSVSAALGFHFIIGAFFGALLVDRNFFLPARYKELEL
                     TLGSITGGFLAPVFFAYLGLEFNVQIIDSFWFVAVVLAVSIATKILAGWLGGRLIGLS
                     RTDAMGIGFILNGRGVMELVIASIAYERGFIGQDLFSVLVLMGVITTLITPLMFRKWV
                     MPHFASRADE"
     gene            complement(3341..4915)
                     /locus_tag="VNZ84_04380"
     CDS             complement(3341..4915)
                     /locus_tag="VNZ84_04380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWU66224.1"
                     /translation="MQRVLFGMLIALWVILMPQASASPTLLGTTCMNSKGVVIASIPL
                     QGGKFVCQSVLKITHADQYVIDFKNTSTIAHFQHEITDSNQQTTILKGGLSSQEKDAF
                     LLRHGHIVHLLPGTYRLVTWLHSPYYLAQPEIFVANLATYQQQTNFTTATSLLLLGVL
                     LGIFTYYFAIGVTPGHATERMYALFILGNLIFQSAALGVFKQLFEWHWFYLTSLPILF
                     SNLAYISFVCHLLGISQYHHAKLYHLSLIVRLALVALLVTAMIFPNWMLEMDRFGVAI
                     FLVFGLTCGLRLSVQKHRVAQLYLLAITVFGILGGLSITAQRLSTNAWTIEQMGLIAV
                     AVEAVMLSLIVAYQIHRLHKEKEHMLAELQSTRSLAMTDRLTGVPNRHALENQMLHFP
                     EHGCLMFLDMDNLKYVNDHFGHEAGDQLLKSFSQILVKKLDTHGQLYRLGGDEFAIIC
                     HEDNIEWCQHQLDYTHMQMEKEGFSGVGTSIGIVFSHESEDTEELMRIADKRMYANKR
                     TRKNRKEMIQVLASQP"
     gene            complement(5092..5703)
                     /locus_tag="VNZ84_04385"
     CDS             complement(5092..5703)
                     /locus_tag="VNZ84_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HWU66225.1"
                     /translation="MFTTITSRKSLGLGLALVLSLSACFKSNPPVKTERYSLPEMTAP
                     ASTSPATTATETLVLNRIQLADYLDTDRIVIQLDDITMHPARDHLWVDSLSQELRRGL
                     RARLSSRLSPVSVVDPQPGLPATARSLNITIDQFQGQMQGYAVLSGQWQFAGSSLPAQ
                     SFKFTTPLAEDGYPALVRALGHNLDLLCEQIASQWQTAPFAAK"
     gene            complement(5724..7394)
                     /gene="pqiB"
                     /locus_tag="VNZ84_04390"
     CDS             complement(5724..7394)
                     /gene="pqiB"
                     /locus_tag="VNZ84_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HWU66226.1"
                     /translation="MTENNHLLANVSKQAKISPIWIVPIVALLIGLWLIYDNYTKAGK
                     QITLSMTNAEGIEAGKTKIKVHSVDIGLVEQVTLSEDLSHIEVIARITPKAQPMLVQD
                     TQLWVVKPRIGLEGISGLNTVISGSYIQLQPGKSLEETDQFTVLDQPPISLNGAKGVH
                     VNLIGKVGNSLRVGDPVTYQGLRVGRVVSAVFDPKQRQMQHELFIEHPYDVLVTENSR
                     FWTTVGIDVKLDSEGFKANIPTVESLIAGGVSFGHADDRPMGKPVVSETTFELYANED
                     AARQQTYDQYLEYVLLVEDTVRGLSKGAPVEFRGLRVGTVVSVPWKFNSPQRKKDLHY
                     AIPVLIHLEPGRLADNGKADLEEWKNRIAHMLQRGLHATLKSGNLITGSVFVDLNMDH
                     RAKTAYHNEHFEGLPVMPTSATGLAQIEQKLSDLLDKLNRLNVEPVLSGLDKNLQQSE
                     KTMQEIRTLSQQVNQLLSQPDLQQMPANINLTMQELRTTMQETRTMLKGLSPESPAYQ
                     ELTNTLQRLDKALRDIQPLARTLNEQPNAILFDRKPGNDPVPMAPVKP"
     gene            complement(7381..8661)
                     /locus_tag="VNZ84_04395"
     CDS             complement(7381..8661)
                     /locus_tag="VNZ84_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HWU66227.1"
                     /translation="MITQPKTKWRRLRACSACDMVSAIPVLRPGEHACCPRCGHVLAR
                     RHLHPVQRSIALATSALLCLLIAVAFPFISMDTNGLDTQIELPQTAITLVDVHQPIVA
                     LIVSCTAILLPALYLIGVILMQANILRRVPRAYSDVIARLFSRLHPWMMADVFMIAAL
                     VSLVKLTEIGEIRLGASFWAYCVFAVLLLMTTRSVDIDWLWFRLAGEPPPDTHIHCGK
                     SAAEQHATGCTTCGLVNTLPDNAVEPNYDCIRCGSTLHMRKPKSLEYTWALLLTAVIL
                     YIPANAYPMMITTSLGQTHASTIIGGMLELWQHGSEPIAVVIFVASVLVPIIKVLILV
                     LLSLLARHTGPLNAHQKTRLYRLTELIGRWSMVDVFVVAIMVALIQSGNLMSVFPGPA
                     ALAFCAVVITTMLAAMTFDPRLLWDTQGKIHDRE"
     gene            8924..9505
                     /locus_tag="VNZ84_04400"
     CDS             8924..9505
                     /locus_tag="VNZ84_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWU66228.1"
                     /translation="MRTEATIKSHKQPSANKQSHPVKHDAIVLLKQDHDEVKKMFKQY
                     QKLAQKGDTDGKVLIANKICAELIAHTIAEEEIFYPAARQAIHEDGMFNEAKVEHDSA
                     KDLIAQMQSMTPEDPMYDAKVTVLGEYIEHHVEEEESEMFPMVRKSKKVDLRQMGSLL
                     ASRKEEVMQELCDMQGEIDTQQLQAKLGMKAKH"
     gene            complement(9591..9743)
                     /locus_tag="VNZ84_04405"
     CDS             complement(9591..9743)
                     /locus_tag="VNZ84_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2474 domain-containing protein"
                     /protein_id="HWU66229.1"
                     /translation="MHNEPKHHIAKNSLTKRLGWLLLIWIVSVGVLAIASLSIKLLMQ
                     IAGFHA"
     gene            complement(9743..10744)
                     /gene="cydB"
                     /locus_tag="VNZ84_04410"
     CDS             complement(9743..10744)
                     /gene="cydB"
                     /locus_tag="VNZ84_04410"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HWU66230.1"
                     /translation="MDLSLVWAIIILFGIMMYIIMDGFDLGIGILYPFFQEKEDRDLM
                     MNTVAPIWDGNETWLVLGGAGLLAAFPLAYSVILSALSLPLLFMLMALIFRGVAFEFR
                     FRANETERHFWDKAFLGGSIAATFFQGMTLGAYLQGLKVVNQAYAGGALDWISPFSLF
                     TGLGLLVTYALLGSSWLIFKTSGELQATCYKLSQRISIALFAIIVVISIWTPLMDSRI
                     AERWFTMPNLLWLSPVPLLVAFVFYKLQAALKAKAETTPFLYTLCLVLLGYAGLGISI
                     WPNIIPPDISIQAAAAPPQSQAFALVGAVIIIPVILAYTAWGYYVFRGKVKPGEAYH"
     gene            complement(10745..12175)
                     /locus_tag="VNZ84_04415"
     CDS             complement(10745..12175)
                     /locus_tag="VNZ84_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWU66231.1"
                     /translation="MSDLALTLARIQFGFTISFHIVFPAITIGLASYLAVLEASWLKT
                     KQTVYLDLYHFWVKLFSLCFGMGVVSGLVMAYQFGTNWSGFSNVAGSVTGPLLAYEVL
                     TAFFLEAGFLGVMLFGWNKVGPTLHFIATLMVAIGTLVSATWILASNSWMQTPQGFEL
                     VNGKVIPVDWLKIIFNPSFPYRLMHMVIAAYLATALFVSAAAAWQLLRGKNNPAVRKM
                     MSMGLWLILLIAPLQVVIGDMHGLNTLKHQPAKIAAIEAHWENKGSEPTPLVLLAWPD
                     MQQEKNHFALEIPVLGSLILRHSLTEQIPALKEFAADERPNATIVFWTFRIMVGLGML
                     MILLAVTSLWLRYKNQLYQHKPFLRFAVAMGPAGVIAILAGWLTTEIGRQPWVVYGIL
                     RTADAVTPHATTEVAVTLALFVLIYLAVFGMGTMYVLRLINTGPKTGEALQGDAISIN
                     PSEVDEFALAPEFHPDAKTPSAGKDH"
     gene            12589..13095
                     /locus_tag="VNZ84_04420"
     CDS             12589..13095
                     /locus_tag="VNZ84_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66232.1"
                     /translation="MTTVTGLFRDQDSAERAYLSATQAGYSASDINVIMSQETRERYY
                     GKQATLETEVGNKAAEGAAVGGALGATAGAIAAAIAAAGTVLALPGLGLVIAGPLAAG
                     VAGAGAGGVTAGLVGALIGWGIPEDTLKEYETDVKNGGILIGLNSKNDEDARRFQSEW
                     NTPVVAGA"
BASE COUNT         3262 a         3200 c         3602 g         3132 t
ORIGIN      
        1 atggactctg taagctccag ttcgagtagc tgtgggtcta gcccggtttt ttccagcgct
       61 ttcacaatga ctttggccag attgccgcgg cggaattgaa ccgcagacac gttgacggcc
      121 atgctcagtg gcggcaagcc atttttctgc cattccatgg cctggcggca ggcttcttca
      181 atgatccatt cacccatttc tacaatgagg ccggtttgct cggcaatgct gataaattgt
      241 gcaggcggct gcataccatt ttgtgggtga tgccagcgga ttagggcttc ggccccgatg
      301 acttgtgcat ttttaaggct gatttgcggc tggtaatgca gttcaaactg gtgatgcgcc
      361 aatgcctcgc gcaggccata agcaatattt aaataatgca ggctatcggt attgagtttt
      421 tcggtaaaaa aacgggccgt gttccggcct tcttccttag cgtgcgacat ggctttgcgc
      481 gcgcggccaa acagtttttc aaagtcgttg ccatcagtgg gataaacggc gaccccaatc
      541 gagcatgagg tagaaatgtt gctgccagca aaagcaaacg gcgatgacac ctcagtcaac
      601 agcatatcca ccatatagtt gatggaatca tcatcgtgca tattgttgag caggagaata
      661 aattcgtcac cgccgtggcg gcagatggta tctgtttcgt gcaaacagcc tttgagccgc
      721 tcggcaaata gcttgagcag tttgtcgccc gaagcataac ccagtgagtc attaaggaac
      781 ttgaaattat ccaggttgag cgccagcagg gcgatttttt gattcgctgc tgctgcgatg
      841 gaaattgcat tctccagcct gtccctgaag tgggtaatgt cgggtaggcc tgtcagtgca
      901 tcgtgcgatg ccaggtactg aatttctttt tgcgcggact tcaggtcact caggtcactg
      961 aatgtgccca cgtaatggct gacttcaccg ctttcatttt taacttctga aatagatagc
     1021 cactcgggga gaatttcgcc atttttgcgt cggttccaaa tttcaccccg ccaatggcca
     1081 aagtcgttca cgctttccca caggttacga taaaagctgc tgtcatgtag gccagaggag
     1141 aggattttag gcgttttgcc tacgacatcg tccagtgtga agcccagcat tctggtaaaa
     1201 gccggattga cggacagaat cacatgcttg gcatcggtca ccataatgcc ttgttcactg
     1261 ccttcgaata ctttttcata ctggcgcaag ctttcctggt atcggttccg gttaagcaca
     1321 atgcctataa tggaagagcc gatttccagc agcatgcggt gaaattgagt gggttcgcgg
     1381 tgctcataac tggagagggc aaaagtacca atcaccttgt tttcggcaga gaagatggga
     1441 atcgaccagc aagccaggat attcaggtca tcgaccaggt gccgaatctc gctccagcgt
     1501 tcatcggtca gggtattgct gacatattgg actgcttgct ggtaaatgac attgccacag
     1561 gagccaccac cgggactagg atgcaaaccg ttgatttttt ggatttgttc tggtgttagg
     1621 ctgggcgcga catagacatt caggtgtacg tatttttcat ccatcagcat gactgagctg
     1681 aatgaattgg gcaccagact ctctgccagc aggcaggttt ggttgatgac ttcgagatgg
     1741 tctgcaccgc gcgcaatcga cttcaggatg gcgcgctgca agttgagtag ctgggtttgt
     1801 tcctcaagag acaatggacc actgtgcgtg atcccagcat gagatactga tgacgctgtg
     1861 gtgatcgaag gtgcgctcac agggggatcc aatatttaat agaggaattc gtttgtgaac
     1921 tagtgttctc cttttgtgaa ttgatgtcaa tagcccgcta ttcgtctgct cgtgacgcaa
     1981 aatggggcat cacccattta cggaacatga gcggtgtaat tagggtggtg atcacgccca
     2041 tgagcaccag taccgagaac aggtcctggc caataaagcc gcgttcgtag gcaatgctgg
     2101 cgataaccag ctccatcacg ccgcggccat tgaggataaa gcctatgccc atggcatcgg
     2161 ttctggacaa gccaatcagg cggccaccta accagccagc aaggattttc gtagcaatgg
     2221 aaacggccag caccacggcc acgaaccaga atgagtctat gatttgtacg ttgaattcca
     2281 ggcccaggta agcaaaaaat accggggcca aaaaaccgcc tgtaatagat cccagtgtca
     2341 gttccagctc tttatagcgc gccgggagga agaagttgcg gtctaccagc aaggcaccaa
     2401 aaaatgcgcc gatgatgaag tgaaagccca gggcagcact cacggagcca aacaccaata
     2461 ccagtaataa cagcaggcca aacaaggctt cattgccgat gaattcgacc agtttttcag
     2521 accattggtc gatacggacc cctttttcgg tgactttttt aatggccagg ctgacgctga
     2581 caatgagcag gatcagcatg attagtttcc agctggcact gaaaatcgca ctgccgactg
     2641 cctggtagga tttttgttct ggcaggttaa gaatcacccc caacaccagc aaggcggcga
     2701 catcgttaaa aatggcggtg gctaccgagt agcgtgcaat attggaatcg agtaacttga
     2761 agctttgcag gatgctgacg gtgaccggta gcgcagtgat tgacacacac aagccaagaa
     2821 agatggtgcg gttgacgtcc aggtcaaagc cgatcccaac caggatgccg cccgtgagcg
     2881 gcagcaggaa acccagcaag gcaatgacaa tgcctttgcc acgcatggaa tctaccactt
     2941 ccttgaagtt catttccagg ccggctgaca gcacgatcag gaacacggca aactctgcaa
     3001 ggccagacag ggcaggggta ggtgaaatca agttgaatat gctggggcct aaaaccaccc
     3061 ctgccagcat ttcgccgatg atggcaggct ggttgaaacg gctaaacacc tggcccatca
     3121 ggcgggcggt gacaatcagg ataagcaggg tggtcagtaa cggcatggtg ctctctttgt
     3181 tttcttacca ggcagtgtgg ggtgcgtctg cgggtgatac tgatgctgta gatacagcgc
     3241 tgtatgtata ttgatttaga gtgtaaatca tttacgcaag ctgcggcaca cttttacttg
     3301 tgaaaagaga gggagaggga atcaggctgg cgcggcttgc ttaaggctgg ctggcaagga
     3361 cttgaatcat ttccttgcgg ttttttcgtg tgcgcttgtt ggcatacatg cgtttgtctg
     3421 caatgcgcat cagctcttca gtgtcttcgg attcgtggct aaagacgata ccgatgctgg
     3481 tgcctacgcc agaaaagcct tctttttcca tttgcatatg ggtgtaatcc agctggtgct
     3541 ggcaccattc gatattgtct tcatggcaga tgatggcaaa ctcatcgccg cccaggcggt
     3601 agagctggcc atgcgtatcc agttttttga ccaggatctg gctaaagctt ttgagcagtt
     3661 ggtcgccggc ttcatggcca aaatggtcat tcacatattt gaggttgtcc atatccagga
     3721 acatcaggca gccatgctcc gggaaatgca gcatctggtt ttcgagcgcg tgccggttgg
     3781 ggacgccggt caggcggtcg gtcatggcca atgagcgcgt gctttgcagc tcggccagca
     3841 tgtgttcttt ttctttatgc aggcggtgaa tctggtaagc cacgatcagg gagagcatca
     3901 ctgcttccac ggcgacagcg atcagcccca tctgttctat ggtccaggca ttggtagaga
     3961 ggcgctgggc agtgatagag aggccgccaa ggataccgaa caccgtaatg gccagcaggt
     4021 agagttgcgc gacacggtgt ttttgtacgc tgagccgcag accacaggtt aagccaaaca
     4081 ccaggaagat cgccacgcca aagcggtcca tttccagcat ccagttgggg aagatcatgg
     4141 cggttaccag cagtgcgacc agtgccaggc ggacgatgag ggacaagtgg taaagcttgg
     4201 cgtgatggta ctggctgatg ccgagcaaat ggcagacaaa gctgatgtag gcaaggtttg
     4261 agaacaggat aggcaaactg gtcaggtaaa accagtgcca ttcaaagagc tgcttgaaga
     4321 cgcccaatgc ggcactctgg aaaatcaggt tgcccaggat aaacaaggca tacatacgct
     4381 cggtagcatg gcccggcgtg acaccaatgg cgaagtaata ggtaaatatg cctaataaaa
     4441 cgcccagcaa taacaggctg gtcgcggtgg tgaagttggt ttgctgctgg taggtcgcca
     4501 ggttggcgac aaagatttca ggttgtgcca ggtagtaagg tgagtgcagc caggtgacca
     4561 ggcggtaagt gcccggtagc aagtggacaa tatgcccatg cctgagcagg aaggcatctt
     4621 tttcttggct actcaagccg cccttgagga tggtggtttg ctggttgctg tcggtgattt
     4681 cgtgctggaa atgcgcaatg gtggaggtgt tcttgaagtc aatcacatac tggtctgcgt
     4741 gcgtgatttt caggacgctc tgacagacaa acttaccccc ttgcagcgga atgctggcga
     4801 tgaccacgcc cttgctgttc atgcaggtgg tgcccagcag cgttggactg gctgaagcct
     4861 gtggcatcag tatcacccat aatgcaatca gcatcccaaa gagtacacgt tgcaaaccca
     4921 ttgttcctga agtgtcaaat tacgttggag tgtacaggaa aagttccttc cgccaagagg
     4981 ctgcaaagca ttaagaaagg caaagaatta ctaactaacg tctaatagct gccgcaagca
     5041 gggttgtttg tggtgcagga cttgattttt tacgacgtta gagctggctt gtcattttgc
     5101 cgcgaatggg gcagtttgcc attggctggc aatctgttcg cagagcaaat ccaggttgtg
     5161 ccccagggcg cgtaccaggg ccggataacc atcctcagcc agtggggttg taaacttgaa
     5221 ggattgggcg ggcaggcttg agcctgcgaa ttgccattgc ccgctgagca cggcatagcc
     5281 ttgcatctgt ccttgaaact ggtctatggt gatattgagt gagcgggccg tcgcaggcaa
     5341 gcccggttgc ggatcaacga cactgacagg gctcaggcgg ctggacaggc gtgcacgcag
     5401 gccacggcgc aattcctgcg acaggctgtc gacccataga tggtcacgcg caggatgcat
     5461 ggtaatgtca tcaagctgta tcacaatgcg gtcggtgtcc aggtaatccg ccagctggat
     5521 acggtttaac accagcgttt ctgtggcggt ggtggccggg ctggtggatg ctggtgcggt
     5581 catttccggc aagctgtagc gttcggtttt taccggcgga ttggatttaa aacaggcact
     5641 gaggctcagc accagtgcta gccccagccc gagagatttt cttgaagtga ttgtggtaaa
     5701 catgttcata agtagtcagt tccttacggt ttgaccgggg ccatgggcac cggatcattc
     5761 ccgggttttc ggtcaaacag gatggcattc ggctgttcgt tcagggtgcg tgccaatggc
     5821 tggatgtcgc gcaaggcttt atccaggcgt tgcaaggtgt tcgtgagttc ctggtaagca
     5881 ggcgattccg gcgacaggcc tttcagcatc gtgcgtgttt cctgcatggt agtccgcaac
     5941 tcctgcatgg tcaggttgat gttggcgggc atttgctgca agtcaggctg gctaagcaac
     6001 tggttgacct gttggctcaa ggtgcggatt tcctgcatgg ttttttcaga ttgctgcaga
     6061 tttttatcca ggccggaaag cacgggttct acattcaggc gattgagctt gtccagcagg
     6121 tcagacagct tttgttcaat ctgtgccaag ccggttgcac tggttggcat gactggcaag
     6181 ccttcgaagt gttcattatg gtaagcggtt tttgcacggt gatccatatt caggtcgaca
     6241 aacaccgagc ctgtgatcag gttgccggat ttgagcgtcg catgcagtcc gcgctgcaac
     6301 atatgggcga tcctgttttt ccattcctcg aggtccgcct tgccattgtc tgccaggcgg
     6361 ccaggttcca ggtggatcaa aaccgggatc gcataatgca agtccttttt acgctgtggt
     6421 gagttgaact tccacggtac actgacaaca gtgcctacgc gcaagcccct gaattccacg
     6481 ggcgcgcctt tggataagcc gcgtacagtg tcctccacca gcagcacata ctccaggtac
     6541 tggtcgtagg tttgctggcg tgcggcgtct tcattcgcat acagctcgaa tgtggtttcg
     6601 gagacaacgg gtttgcccat aggacggtca tcggcatgac caaaactgac gccaccggca
     6661 atcagtgact ccacagtcgg gatgttggcc ttgaagcctt cggagtctag tttgacatcg
     6721 atgccaaccg tggtccaaaa gcgtgagttt tccgtgacaa ggacatcgta aggatgttcg
     6781 ataaacagct catgttgcat ttggcgttgt tttgggtcaa acactgcgct gacgacgcgg
     6841 ccgacacgca ggccctggta agtgaccgga tcacccacac gcaaggagtt acccactttg
     6901 ccgatgaggt tcacatgcac acctttagcc ccattcagcg aaataggcgg ttggtccagc
     6961 acggtaaact ggtcggtttc ttccaggctt ttacctggtt gtaattgaat gtaagacccg
     7021 gaaatcacgg tattcaggcc actgatgcct tccagcccaa tccgcggttt gacgacccag
     7081 agctgggtat cctggaccag catgggttgg gctttgggtg tgatgcgtgc gatcacctca
     7141 atatggctca ggtcttcaga caaggtgacc tgttccacca ggccaatatc cacactatgc
     7201 accttgattt tggttttgcc ggcctctatg ccttcggcat tggtcatgga cagcgtaatt
     7261 tgcttgccag ctttggtgta attgtcataa atcagccata agccgattag cagcgccacg
     7321 atcggcacaa tccagatggg cgaaattttg gcttgtttgc tgacgttggc cagcaaatgg
     7381 ttattctctg tcatgtatct ttccttgcgt atcccagagc aggcgtgggt caaatgtcat
     7441 ggctgccagc atggtggtaa tcaccacggc acaaaaggcg agtgcagccg ggcctggaaa
     7501 aaccgacatc aaattgcccg actgtatcaa ggccaccatg atggcgacga caaacacatc
     7561 gaccatggac cagcggccta tgagttcagt caggcgatat agcctggttt tttgatgtgc
     7621 attcaacggg cccgtatgcc gcgccagtaa agaaagcaac actaggatca gtactttaat
     7681 aatgggcacc agcacgctgg ccacaaaaat caccactgca atcggttcag agccatgttg
     7741 ccataactcc agcatgccgc caatgatggt ggatgcatgc gtctggccca gggaggtggt
     7801 aatcatcatg ggataggcat tggccggaat atataaaatg acggcagtca gcaataatgc
     7861 ccaggtgtat tccaggctct tcggtttgcg catatgcaag gtgctgccac aacggataca
     7921 gtcataattt ggctcaacgg cattgtcagg cagggtgttg accaggccgc aagtggtaca
     7981 ccctgtggca tgttgttcgg cagcactttt gccgcaatgg atatgggtat caggaggcgg
     8041 ctcaccagcc agcctgaacc ataaccagtc aatatcgacc gagcgtgtgg tcatcaacaa
     8101 cagtactgca aatacacaat aggcccaaaa agaggcgccc aaacggattt caccgatttc
     8161 ggtcagcttc acgaggctga ccagagcggc aatcataaac acatcggcca tcatccaggg
     8221 atgcaagcgc gaaaataggc gggcgattac gtcgctatag gcacgcggta cccggcgcaa
     8281 tatattggct tgcatgagaa taacacctat caggtacagg gctggtaaca ggatggccgt
     8341 gcaactgaca atcagcgcga caatcggctg gtgtacgtcc accagggtga tggccgtctg
     8401 cggcagttca atctgcgtgt ccagcccatt ggtatccatg ctgatgaacg ggaaggccac
     8461 tgcaatcagc aagcacagca atgcactggt cgccaacgca atactgcgct gtaccggatg
     8521 caaatgacgc ctggccagga cgtgaccgca gcgcggacag caggcatgtt cgccagggcg
     8581 cagcacggga atcgcactga ccatgtcgca agcactgcag gcacgcaaac ggcgccattt
     8641 tgttttgggc tgggtaatca tacggtttga ggaggtaatg atggtattac ctgactttta
     8701 ctagcatggt aaacgggttg gatttgaaca ggcaagaaaa attccattcg ctgccgactt
     8761 cagaagctga ttaatccaag cacctgaagc ttgctttcgc ccgcctttaa agattttctg
     8821 acaagattat cagtgaatcc agcctattgt acgcaggggc ttgaaggcaa tatttgcgtt
     8881 caggaatacc catatcattc aacgaccata tgaggagagt agcatgagaa cggaagcaac
     8941 gataaagtca cataaacagc cgtctgccaa caagcagtct catccggtga aacatgatgc
     9001 catcgtcttg ctgaagcaag atcatgacga agtgaagaag atgtttaaac agtatcagaa
     9061 acttgcccaa aaaggagata cagatggcaa ggtactcatc gcaaataaga tctgtgcaga
     9121 attgattgca cataccattg ccgaagaaga aattttttat cccgctgccc gtcaagctat
     9181 tcatgaggat ggcatgttta acgaggccaa ggttgagcat gatagtgcta aagacctgat
     9241 cgcgcagatg caaagcatga cgcctgagga tcctatgtat gatgccaaag tgaccgtgtt
     9301 aggcgagtac atcgaacacc atgtagaaga ggaagagagt gaaatgtttc ctatggtgcg
     9361 caaatccaaa aaagtcgacc tccggcagat gggctctttg ttggcctctc gcaaagaaga
     9421 ggtgatgcaa gagctttgtg atatgcaagg tgaaattgat acgcagcaat tacaggccaa
     9481 gcttggtatg aaagccaagc actaagcata tgtcacgcaa taaaaaagga gcattcagct
     9541 cctttttttg tatgtggtta ttggttaaag tggcggtttc cgcgtgcgtt tcaggcatga
     9601 aaaccagcta tttgcatcag caatttaatg ctgaggctgg cgatggccag cacacccaca
     9661 ctcactatcc agatcagaag caaccagccc aatcttttag tcaggctgtt tttggcaata
     9721 tggtgtttgg gttcgttgtg cattaatggt aagcctcgcc tggtttgacc ttgccacgaa
     9781 atacgtaata gccccaggcc gtgtaagcga ggatcaccgg gataatgatg acggccccta
     9841 ccagggcaaa ggcctggctt tggggcggtg cggcagcggc ctgtatgctg atgtcgggcg
     9901 ggatgatatt gggccagatg ctgatcccca atcccgcata accgagcaga accagacaca
     9961 aggtataaag aaatggtgtg gtttcagcct tggccttgag cgccgcctgc agtttgtaaa
    10021 agacaaaagc taccagcaaa ggcacgggtg acagccatag caggttaggc atggtaaacc
    10081 agcgctcggc aatccggcta tccataagcg gcgtccagat gctgatcacg acaataatgg
    10141 cgaacagggc aatcgagatg cgttgagaga gtttgtagca cgtcgcttgc aattccccac
    10201 tggttttaaa aatcagccaa ctactgccta gcagtgcata ggtcaccagc aggcctaatc
    10261 cggtaaacag tgaaaacggg gatatccagt ctaaagcccc acctgcgtaa gcctggttaa
    10321 ccactttaag cccttgcagg taagcgccca gcgtcatgcc ctggaagaaa gtggctgcaa
    10381 tcgaaccgcc caaaaaggct ttatcccaaa agtgccgctc cgtttcattg gcgcgaaacc
    10441 tgaactcaaa agccacgccg cggaaaatca gtgccatcag cataaacagc aagggcaggg
    10501 aaagggcact taaaatcact gaatatgcga gcggaaatgc tgccagcagg ccagcgccac
    10561 caaggaccag ccaggtttca ttgccgtccc agatcggggc cacggtattc atcatgaggt
    10621 ctcggtcttc tttttcctgg aaaaaggggt acaggatacc gatgccgaga tcaaagccat
    10681 ccatgatgat gtacatcatg atgccaaaca ggataatgat tgcccatacc aacgataagt
    10741 ccatttagtg atcctttccg gcagagggtg ttttcgcatc tgggtgaaac tcaggtgcca
    10801 gtgcaaactc atccacttcg ctgggattaa tagagatggc atcgccttgc aaagcttcgc
    10861 ccgttttggg cccggtattg attaacctca gcacatacat ggtccccatg ccgaataccg
    10921 ccaggtagat taagacaaaa agtgccagcg tgacggcgac ctcagtggtt gcatgtggtg
    10981 tgacggcatc tgccgtacgt aaaatgccat agacgaccca aggctggcgg cctatttcag
    11041 tggtcagcca gcctgccagg atggcaatca cgccggccgg tcccatggcg actgcaaaac
    11101 ggaggaaagg cttatgctga tacagttggt ttttatagcg taaccataat gaggtcacgg
    11161 ccagcaagat catcagcatg cccaggccga ccatgatgcg gaaggtccag aacacgatgg
    11221 tcgcattcgg cctttcatcc gctgcaaact ctttaagcgc gggaatctgt tccgtcagtg
    11281 agtgacgcag gatcaggctg cccaggaccg gaatctccag cgcaaaatga ttcttttcct
    11341 gttgcatgtc cggccaggcg agcagcacta aaggcgttgg ttcactccct ttgttttccc
    11401 agtgcgcttc gatcgcggca atttttgccg gttggtgttt taaggtgttg aggccgtgca
    11461 tgtcgccaat caccacttgc aacggggcaa tcagcaatat cagccataaa cccatagaca
    11521 tcattttgcg taccgccgga ttatttttgc cacgtaacag ttgccaggcc gctgcagccg
    11581 atacaaacag cgcggtagct agataggctg caatcaccat gtgcatcagg cggtagggga
    11641 aagagggatt gaagatgatt tttagccaat ctaccgggat gactttgccg ttgaccagtt
    11701 caaaaccttg cggtgtttgc atccagctgt tggatgccaa gatccaggta gccgatacca
    11761 gggtgcctat agcaaccatg agcgtggcga taaagtgcaa ggttggcccg accttgttcc
    11821 agccaaatag catcaccccg agaaaacctg cttccaggaa aaaggcagtc agcacttcgt
    11881 acgcaagcaa cggcccggtg acgctccctg caacgttaga gaatcccgac cagttggtgc
    11941 caaattggta ggccatgacc agcccggaaa ccacgcccat gccaaaacat aaagagaata
    12001 atttgaccca gaaatgatat aggtccaggt agaccgtctg cttggttttt aaccagctgg
    12061 cctctaggac tgctaaatag cttgccagcc caatggtgat cgccgggaag acgatatgaa
    12121 acgaaatggt gaatccaaac tggattctcg ctaacgtcaa tgccaaatca gacatacatg
    12181 ccctttacat cacttgcgac acattgcgtg cgtcagctga ttagagaaaa gctgaccgca
    12241 aatactacca gcattgacta ccattcagcg tattaagttt gcctgacatg gcgggattta
    12301 cagaaattgt gtagcaatgc ttgtggacat taggttaggt tgatttcaaa aaagagttaa
    12361 atgcatcgag tctgacccca ttgatttcat aagttattta ttcttttttg ctattgattt
    12421 ccttaacaga ttagcttgtc tccatggcga gcttcccttc tcggcttaag tgttcaacct
    12481 ccttcatcat cattatccta cagccatatc agcgccatca cactcgttcg cccgaatcgc
    12541 gtttcatata ctgtcttttc ctataagaaa caatttttgg agatgactat gactacagta
    12601 acgggtttat ttagagatca agacagtgca gagcgcgctt atctttcagc cactcaagcg
    12661 ggttacagcg caagtgatat taacgtgatc atgtctcagg aaacacgaga gcgttattac
    12721 ggtaagcagg ctactttgga aaccgaagtg ggtaataaag cagctgaagg ggctgcggtg
    12781 ggtggtgcgc ttggcgccac ggctggggcg attgcggcgg ctatagcggc tgcgggcacc
    12841 gttctggctt tgcctggctt agggcttgtg attgctggtc cgttagcggc gggtgtcgca
    12901 ggtgcgggag cgggtggtgt gaccgctggc ttggtcggtg ccttgattgg gtgggggatt
    12961 cctgaagata ctttgaagga atacgaaaca gatgttaaaa atggcgggat tctgattggc
    13021 ttgaactcaa agaatgatga agatgcccgt cgttttcaaa gtgaatggaa tacacctgtc
    13081 gttgctggcg cataataggc cttagtttta atgatgacct aaaagcaaaa agccttgcat
    13141 ttctgcaagg ctttttttat ttggctcctc aacctgggct cgaaccaggg acctac
//
LOCUS       DATMSK010000130         3318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_56933,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000130 DATMSK010000000
VERSION     DATMSK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56933"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VNZ84_04425"
     CDS             complement(<1..299)
                     /locus_tag="VNZ84_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU66233.1"
                     /translation="MKKWWMAVAMWLVSSVAMAGMSPDQLVKKTADDVIEVIKSDKDI
                     QAGNQQKIFALAEEKILPNFDFEKVSRLVLGKNWTNATPEQKTAFQAEFKTLLLRT"
     gene            complement(326..871)
                     /gene="mlaD"
                     /locus_tag="VNZ84_04430"
     CDS             complement(326..871)
                     /gene="mlaD"
                     /locus_tag="VNZ84_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HWU66234.1"
                     /translation="MERTTIDLWVGIFVALGLAALLGLAMKVGNLTTSSIGDTYMVTA
                     NFENIGGLKPRAPVKSAGVVVGRVGDIGFDAKTYEAVVSLNIDKRYNFPKDTFANIYT
                     AGLLGEQYIGLEAGGDEENLKNGDKITQTQDAVVLEKLISQFLYSKATEGDDKANKTA
                     STSTESGTDALDASPLDKIGK"
     gene            complement(898..1692)
                     /gene="mlaE"
                     /locus_tag="VNZ84_04435"
     CDS             complement(898..1692)
                     /gene="mlaE"
                     /locus_tag="VNZ84_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HWU66235.1"
                     /translation="MVVHKLKRLLSKLGAGCINKIWRLGAAARLFGLTLISSGESFRR
                     FRLTIREVYFTGVLSLIIILVSAFFVGMVLALQGYHTLQKYGSSEAIGVLVALALVRE
                     LGPVVTALLFAGRAGTAITAEIGLMKATEQLSAMEMMAVSPIARVIAPRFWAGVIAMP
                     VLATLFSMVGILGGYLVAVPLIGVDAGAFWSQMQANVDWEFDILNGVFKSLVFGVACT
                     MIALFEGYDAPPTAEGVSRATTRTVVTSSLAVLGLDFILTSFMIAV"
     gene            complement(1692..2495)
                     /locus_tag="VNZ84_04440"
     CDS             complement(1692..2495)
                     /locus_tag="VNZ84_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU66236.1"
                     /translation="MANAIVEIQDLSFGYKGRLLHKGINMTFPKGKVVAIMGGSGSGK
                     TTLLRLIGGQLKPTKGQVTVCDEVVHRQDREGIYRLRRKMGMLFQHGALFTDLSVYEN
                     VAFPMREHTNLPESMIRDLVLMKLNAVGLRGAHALMPTELSGGMARRVALARAIALDP
                     SIIMYDEPFAGLDPISMAVICDLIRSLNDALGATSIIVTHDVEETFQFADYVYFVADG
                     VVAAEGTPEELRQSELPFIHQFVHGEKDGPVPFHYAAPEYHASLLRGAR"
     gene            complement(2625..3263)
                     /locus_tag="VNZ84_04445"
     CDS             complement(2625..3263)
                     /locus_tag="VNZ84_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWU66237.1"
                     /translation="MKKEHGFSLIELAIVLVIAGLLLAGVMRGQELIANAKVKSLASD
                     FRNIPTYFYGYQDRFRVLPGDDHAADIHVAGTVASTTGQIQNGLIQGVWNSATNTDES
                     FLLWQHLRLAGLATGATDVGNAQYAPRNSEGGQLGVTSVAALAAVTAGTFNSAHAMCS
                     DGILGRYAQQLDTMLDDGSGNTGAMRILINGTPGTGEAAPAAGTAYLVCMSF"
BASE COUNT          906 a          944 c          662 g          806 t
ORIGIN      
        1 gtacgcaaca gcaaagtctt gaattctgcc tggaatgctg ttttttgctc aggcgttgca
       61 ttggtccagt ttttacctaa taccagacgg gaaacttttt caaagtcaaa gtttggcagt
      121 attttttctt cagccagcgc aaaaatcttt tgttggttac ctgcctgaat gtctttatca
      181 ctcttgatga cttcgatcac atcatcagcg gtttttttca ccaactgatc cggcgacata
      241 ccagccattg ccacactgct tacgagccac atggcaactg ccatccacca ttttttcatc
      301 gctttccctt taccaattgt ttgaattatt taccaatttt gtccaatgga ctggcatcta
      361 acgcatcagt accagactcg gttgatgttg atgccgtttt atttgcttta tcatcaccct
      421 ctgtggcttt gctatacaag aactggctaa tcagcttctc aagcaccacc gcatcctggg
      481 tctgtgtaat cttgtcaccg tttttcagat tctcttcatc gccacctgct tcaaggccaa
      541 tatattgctc gccgagcaag ccagcagtat aaatattggc gaatgtatcc ttggggaagt
      601 tataacgctt gtcgatattc agactcacaa cagcctcata agttttggca tcaaagccaa
      661 tatcccctac acggccaact accacccctg cactttttac tggtgcgcga ggtttgagtc
      721 cgccgatatt ctcaaagttc gccgtcacca tataagtatc gccaatacta ctagtggtca
      781 aattgcccac tttcattgcc aaacctagta aagcggccaa tcccagagca acaaaaatgc
      841 ccacccataa atctattgtt gttctttcca tagtctattc cccgcaaatt cctgacgcta
      901 aaccgcgatc atgaacgacg ttaatataaa atccagcccc agcaccgcca acgaagaagt
      961 caccactgtg cgcgtcgtgg cacggctgac tccttctgcc gtaggtggcg catcataccc
     1021 ttcgaacagg gcaatcatgg tacaggccac gccaaatacc aggctcttaa aaaccccgtt
     1081 caaaatatcg aattcccagt ctacattcgc ctgcatttgc gaccaaaatg caccagcatc
     1141 gacaccaatc aaaggcacag ctacaaggta gccacccaaa ataccgacca tagaaaacaa
     1201 tgtcgccagc acgggcatgg caatcactcc cgcccagaat cgcggtgcaa tcacgcgtgc
     1261 aattgggctt acggccatca tttccatcgc cgataattgc tctgtggctt tcatcaggcc
     1321 aatttctgca gtaatcgccg tacctgcacg accagcaaac aatagcgcag tcaccaccgg
     1381 accgagctcg cgtaccaacg ccaatgccac cagcacgcca atcgcttcgg aggatccgta
     1441 tttttgcaag gtatgatagc cttgcagagc cagcaccatc cctacaaaga atgctgacac
     1501 gaggataata atcagcgaca acacacccgt gaaatacact tcacgaatgg ttaaccgaaa
     1561 acgacgaaag ctttcaccag aggaaatcag ggtaagcccg aacaaccgtg cagcagcacc
     1621 caggcgccag attttattga tgcaaccagc gcctagtttg gacaacaagc gcttgagttt
     1681 atgcacaacc attagcgcgc cccccgcaac aaactggcat gatactccgg tgcagcgtag
     1741 tgaaagggta ccggaccatc cttttcacca tggacaaact ggtggatgaa aggtaattct
     1801 gactgccgta actcttctgg tgtgccttct gcggctacaa caccatcggc tacaaagtag
     1861 acatagtctg caaactggaa ggtctcttcc acatcatgtg tcacaataat ggatgtagca
     1921 cctagcgcat cattgagact gcgaatgaga tcacagataa ctgccattga aatcgggtcc
     1981 aatccggcaa atggctcgtc atacatgata atgctcggat ccagtgcgat ggcccgcgcc
     2041 aaggcaaccc gcctggccat accaccagat aactcggttg gcatcaaggc atgcgcgcct
     2101 cgcaaaccca ctgcattcaa cttcatcagc accagatcgc gaatcatact ttcaggcaaa
     2161 ttggtatgtt cacgcatggg aaatgccacg ttttcgtaaa cggacaaatc agtaaacagg
     2221 gcgccatgct gaaataacat acccattttg cgacgcaagc gataaatacc ctcacggtcc
     2281 tgccgatgaa ccacttcatc acagaccgtc acctgcccct tggttggctt taattgcccg
     2341 ccaatcaagc gtagcaatgt ggttttaccg ctgccactgc cacccatgat ggcaaccact
     2401 ttgcccttgg gaaaagtcat attgatgcct ttgtgcaaca atcggccttt atacccgaaa
     2461 gacaggtcct gaatttctac aattgcgttt gccataggag atggttgatg taaaacggct
     2521 cttattctaa aacagaatgg acttaaagta gtgtatgtat cctacataaa acaaaaagcc
     2581 tgaaaaacca ggccttttgt tacaaattta tgttgtattc tacactaaaa actcatgcac
     2641 actaagtacg ccgtccctgc tgcaggtgcc gcctctcccg taccaggcgt gccattgatc
     2701 aatatccgca ttgcgccagt attgcctgaa ccatcatcca gcatggtatc caactgctgc
     2761 gcataacgcc ctaaaatccc gtccgaacac atggcgtgcg cactgttaaa agtaccagcc
     2821 gtgaccgcag ctagtgcagc aacactggtg acaccaagct ggccaccttc actattccga
     2881 ggcgcatatt gcgcgttacc gacatccgtt gcgccagtag ccaatcccgc caatcgtaaa
     2941 tgttgccaca ataaaaatga ctcatctgta ttggttgctg aattccagac accttgtatc
     3001 aagccgtttt gaatctgccc agtggtgctg gcaaccgtgc cggccacatg gatgtctgcc
     3061 gcatgatcat ccccaggcaa cacacggaac ctgtcttggt agccgtaaaa gtaggtagga
     3121 atattccgga aatcactggc cagacttttc actttcgcat tcgcaatcaa ctcctgacca
     3181 cgcatgaccc ctgccagcaa caggccggca atcaccagca caatggccaa ttcaattaaa
     3241 ctaaatccat gctccttttt catctctaac tcccctgtat taaatgataa agatatgatt
     3301 tgagaccgca actaccag
//
LOCUS       DATMSK010000131        13718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_385846,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000131 DATMSK010000000
VERSION     DATMSK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 13718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13718
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385846"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..502)
                     /locus_tag="VNZ84_04450"
     CDS             complement(122..502)
                     /locus_tag="VNZ84_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HWU66238.1"
                     /translation="MSRFQTAAARILLGLVFFGVVILKLMAILNTPDGYLQYQMMLGQ
                     FGLPAVFAPLLILIQFVFGLMLILGFKTQLSARVLGGLAVFMALVLGQASLDAFFAYM
                     GIAGGMLLLSVYPQTACSLDNRSK"
     gene            586..1026
                     /locus_tag="VNZ84_04455"
     CDS             586..1026
                     /locus_tag="VNZ84_04455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU66239.1"
                     /translation="MSVQHTKLMIHTLAMHQPLWPECCRIRQRVFIEEQHVPVDLEWD
                     ETDEAAIHLLASLNGQAVGCARVMPEGYIGRMAVLPAWRGKGIGEALLLQAIQMCRQF
                     DVSHARLSAQMHAIGFYQQAGFEVCSEPYSDADILHVDMQLELV"
     gene            1074..1616
                     /gene="fae"
                     /locus_tag="VNZ84_04460"
     CDS             1074..1616
                     /gene="fae"
                     /locus_tag="VNZ84_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HWU66240.1"
                     /translation="MSKIVMKAGEATVFSEGKDVTAAMPEIVIGSVDGPVGTAFANMM
                     AQTKGHTAMFAVRDINQMVRPATMMVPKVTLKDSLNIELFGGVVQAGVADGITDAVIE
                     GIIPKELVNDLCIVALLWIDPGCAKEANLDKADLYKNNYEAIKLALKRALNDEPSIDE
                     IIANRHKIKHCMWEDSWDQK"
     gene            1676..2083
                     /locus_tag="VNZ84_04465"
     CDS             1676..2083
                     /locus_tag="VNZ84_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWU66241.1"
                     /translation="MKYLMKRYLAVVYEALLMLALTLALTAAYVMLVGDASHGWKRLG
                     LQALLWLTMGAYFVRCWVVTGQTLASQTWKLKVVDRHGQLLSQQQAMLRYILASLLLL
                     PAGLTLWWALLDRDHQFLHDRLLGSRMITLPTA"
     gene            complement(2080..3174)
                     /gene="lptG"
                     /locus_tag="VNZ84_04470"
     CDS             complement(2080..3174)
                     /gene="lptG"
                     /locus_tag="VNZ84_04470"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HWU66242.1"
                     /translation="MTLVILNRYFWKEISFSIMLVLLGLLAMFSFFDLLQELDSLGRG
                     HYGINSMLIYVALSIPGHIYEVAPVAVLLGIVYTLGAMASSSELIVMRTSGISLQTIA
                     KMLLSIGLVFALVTFLIGEIIAPISEKMAQRIRIQATDSVIAQDFQSGLWIKDGSSFV
                     NVNTVMPDTSLVDIRIYDFDKQFRLRTISHADKGYFEDDHWVLSNVTQTKMNTSKAIQ
                     NNSVTSQFFKQTKWESLIRPELLNVLLVLPEKMSAWNLYSFIQYLHQNGQRSTRYQVA
                     LWSKLVYPLACLVMVMLALPFGFLQQRAGGISAKIFTGIFLGVVYQIMNRVFVHLGVL
                     NDWSPLVSAISPTLLFLAAGLGMLYYVERR"
     gene            complement(3171..4280)
                     /gene="lptF"
                     /locus_tag="VNZ84_04475"
     CDS             complement(3171..4280)
                     /gene="lptF"
                     /locus_tag="VNZ84_04475"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HWU66243.1"
                     /translation="MKIFKRALSAELMSSASAIFLIILGIVVAQRAGNIVRQVSRGIL
                     PNDAIGTILTFSLVQFMPMMLSLAIFLAVLLTLTRWHRDSEMVIWMNAGLSLRQWVVP
                     ILRFITPIVLIISLFSLLIMPWANDKADNYRAQLKKRDELSSISPGTFKESNNGERIY
                     FIESFDKLGYEVKNIFVQSKQNGKTGIITANSGSRYKASNGDNFLRMEHGRRYETIPN
                     TLEVTTTEFERYDIRVETKETAPTTSSAQTKSTQSLLAGATAADHAELHWRLAIPIST
                     IVMVLLAIPLSFVDPRAGRSLNIMFAILIFIIYNNMLSIFQAWITQGKISVMVGLWPV
                     HFSFILLGWYLYYRRKHLKPLIPDWFKRRAKRTSS"
     gene            4369..5850
                     /locus_tag="VNZ84_04480"
     CDS             4369..5850
                     /locus_tag="VNZ84_04480"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWU66244.1"
                     /translation="MEFSTKSAAPEKLRTDCLLVGIYEGKKLTDLAKALDTVSEKAIS
                     NALKSGDMEGKAGTTLILRQVAGVVASRVMLVGLGKADEASVKSLRNSFRVALKALPV
                     GVESVATDFASLGIKKITTQQKAAALAEVTLDATYKVNAVKAKSVEPHPLKQVVLLAD
                     KAEIAAVEVGAKQGQGLGQGVALAKDLGNLPPNICTPSYLGKQAEKLAKEYGFKVEVL
                     DQPAIEKLNMGSFLGVTRGSEEPPRFIVLQHLQGKKSQKPVVLVGKGITFDTGGISLK
                     PGADMDEMKYDMCGAASVLGVFKAIGELKLPFNVVGLIPTCENMPSGRATKPGDILTS
                     MSGQTIEVLNTDAEGRLILCDALTYAERFEPAAVIDIATLTGACVIALGHHVSGLFAN
                     NDGLAKALLAAGEQANDRAWQMPLWDDYQGQLDSNFADMANIGGRAAGSITAACFLSR
                     FAKKYDWAHLDIAGTAWKSGKEKGGTGRPVPLLVTYLLEQAAK"
     gene            5856..6275
                     /locus_tag="VNZ84_04485"
     CDS             5856..6275
                     /locus_tag="VNZ84_04485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HWU66245.1"
                     /translation="MTRIRFYTDVTDKIALIQHLVSQALSRQRHVTVYVQDREQAVQL
                     SNGLWQQAAESFFPNALADAGHVALTPVQLVWQPEQVNQDDLLFNCQASLPKFFSRFR
                     HVFELVGTDESDKAAARQRWAFYRDRGYEIQHMNKQV"
     gene            6301..7170
                     /locus_tag="VNZ84_04490"
     CDS             6301..7170
                     /locus_tag="VNZ84_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66246.1"
                     /translation="MDISEIPVLTKVVHKSAVPATDFDELVAQLKEALLPEVAQLVSS
                     QLAKHQPDATTGQQSLAEYANTLQQELMAKAQSQMGDTIQSVEQAFTEAMGNVSKQQL
                     QTFQATLESQSTQQLQAIEEKLSHLTEIQQQLVSSAEQSLRETLEKTTEQSLNDALMP
                     LIERQQAQLEGRVQVLREQLEQGLDVHLQQLQENSRQTLAAGQEVAATELAADFKVSL
                     QQAFAELNASQLSEFRQNMQSELLASETALQEKVAAMVGTQLQEMEAEMNKRLKSRIL
                     EVLQGIKFVMPTI"
     gene            complement(7172..7942)
                     /gene="xth"
                     /locus_tag="VNZ84_04495"
     CDS             complement(7172..7942)
                     /gene="xth"
                     /locus_tag="VNZ84_04495"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWU66247.1"
                     /translation="MKLATWNVNSLTVRLPHVLDWLAANQPDVLCLQETKQEDSKFPY
                     AALKEAGYNAVHNGQKTYNGVAIISPHAIESVHLDLPDFADTQKRIIAATIQDVRVIC
                     AYVVNGQSIDSDKYQYKLNWLGVLHAWVKAELSQHSKLALLGDYNIAPDDRDCHDPAA
                     WQGQVLVSAPEREAFQQLLALGLHDSFRLFNEEAGQFSWWDYRMAAFRRNMGLRIDHI
                     LVSDALKALCQSCVIDKTPRKLERPSDHTPVVLTLANA"
     gene            8142..9326
                     /locus_tag="VNZ84_04500"
     CDS             8142..9326
                     /locus_tag="VNZ84_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU66248.1"
                     /translation="MSRRTASFSRAEWWLVVAVVLVAISLRPGIVSMGPALTAIIENF
                     RLSHATASMMTAIPDVLMGALALPTPWLVRRFGVNRILMLALALLSLSIAVRAYAGTS
                     AVLIIATVGVGAGIAVAGAMFAGIAKASFANHAAFIMGIYATALSLGSTLSAGLTGWM
                     LQLAGWRLATGIWAVFGLIAIVAWGLVIRKSVTTRQASVISSTATAPSRLPLHTPVAW
                     LIALFFACVNFLFYSILTWTAAMYQERGMPAAEAGVVLACFTLCFMLASPVVGMYSRH
                     VDRRGWLVSCALLSTIGLAGMASPGASPWLWVPLTAMGLGGAFTLAMTLPLDHTHSPD
                     AANRWNAFVLTIGYLIAATGPFTMGWMRDVSGQFEGAYQLLVGVAVLMLVIATRLKPP
                     ST"
     gene            9476..13192
                     /locus_tag="VNZ84_04505"
     CDS             9476..13192
                     /locus_tag="VNZ84_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU66249.1"
                     /translation="MKPVSPLWLLRWSLTIVMLAVSVLSSASEEITLQLKWRHQFQFA
                     GYYAAVAQGYYREEGLNVRLVEGSKDLPPLKEVLSGRAEYGIGDAEILVSRISGKPVV
                     ALAAIFQHSPGVLLSLKQSNIQKPEDLIGKRIMLSGDGQGVYQFKAMLLKQHLDISQM
                     TIVPHTWRLQDLIEGKVDVVSAYAMDEPGQLQQMGYETSMISNHPYGVDFYGDVLFTS
                     EREIAMHPERVRAFLRATKKGWLYALSHQEEMAALIAGMPGVTQRGLSKQTLLHEANV
                     MVPYVLTDVVPLGQMHEERWNTIAQMLADLGLVSQHYDLNGFMYQGDMSGKRPYMVWL
                     VRSLSILLLVLGATFVWNVQIRRQVIQRTQALQNEIDRRTQAEYLLKVAGNVAKIGGW
                     VIDLKTNLIHWSDEVAALHEMPPGYSPTVEEGMNMFVPESQPAMAGALKACREAGVPY
                     DLELEKITANGRRFWVRAMGQPVYDQAGNVIKLQGSLQDITAHKQLEAIKQGQDRIQA
                     MMLADIALPDILHAAVQLIEQHLPDAYCAMLLLDQDGSYLRHTASSRLPADYAKAIDG
                     IPLGTCGIAALTEGRVIAEDVTQHPLWEKCCELAMTHGLRACWSMPVLSRSRRVLGVL
                     AIYRKQRHTPGTAELDFIDSYAQILGLAIEREQADEQLHLLQSGISRLNDVVMITEAP
                     MSTPMQQKIVFLNDAFTRITGMPVVQLQGISPFEIFKDATPGRELERIKHAFLHMQPI
                     KTEVVAINQEGVGISLEMDALPLHDKGGRMTHWVAVLRDITERKQADAQIRQLAYYDT
                     MTGLPNRLLLQDKLSSHVRKAMRKQVGGALLFIDIDNFKTLNDTYGHDVGDALLVEVA
                     RRIRQSVRRLDTVSRLGGDEFVVVLDSLHYEPAQAEKQARRVCEKIMAAFKKPFMLKH
                     YQHYTTPSIGVVLFDPEHLSQTDELLRRADLAMYKAKEAGRNGFRFFDTQMETELRHR
                     VALEADLHAAIEKQEFTLHFQPQYNHNHEIVGAEALVRWQHPQHGLIMPGQFIGLAES
                     SGQIGALGGWVLQEACKMLQHWANHPQLKHLVLSVNVSPKQYLQPNFVAEIQQMLDES
                     GVSPGHLKLELTESMLVDNVEDILAKMDALRQIGICFSLDDFGTGYSSLSYLKRFPFD
                     QLKIDQSFVRDMLNGRDHESIVNAIISLGQSLQLEILAEGVETEAQLSVLKDLGCEIF
                     QGYYFTRPLSLEAFETWVIQPDQAVIHTSTAQAV"
     gene            complement(13248..13694)
                     /locus_tag="VNZ84_04510"
     CDS             complement(13248..13694)
                     /locus_tag="VNZ84_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HWU66250.1"
                     /translation="MTSLESTFEQTLFELIPQTRNFGIKIVKINASELIVQGSYAQNK
                     NHLDIVFGGSIAAISITTAWSLLQHNIQQAGLKGKLVIKQQEVKFLLPVHTDFECIAT
                     MSSADAWSQFVDNFHQHGRARMTLSARVVCQAKTTSIFEGVFVLYQ"
BASE COUNT         3394 a         3207 c         3716 g         3401 t
ORIGIN      
        1 taccgtaaac tcaaacagct ggggatcaag attaaaggtt aatcaacaag cgcgaatcca
       61 gatttagtga ataaaaaagc cggaaatctc cggctttttt attctcgtct tgtcaaatga
      121 cttatttgct gcggttatcc aggctgcaag cggtctgcgg ataaacactc aacaacagca
      181 tgcctccggc aatgcccatg tatgcaaaaa atgcatccag tgaggcctgg cctaatacga
      241 gtgccataaa gacagcgagg ccgcctagca cacgtgctga cagttgggtt ttgaaaccaa
      301 gaatcagcat caagccaaac acaaactgaa tcaagatcaa taaaggcgca aacactgctg
      361 gcaagccaaa ctggcctaac atcatctggt attgcaggta accatccggg gtattcaata
      421 tggccatcag ttttaaaatc actacgccaa aaaataccag cccaagcaag attctcgctg
      481 ctgctgtttg aaaacgactc atcacttttc ctcaaatcct gttttaataa aggacgtatg
      541 atgcctgtcg ccagttacaa ttgcaatatt tcagccggag cgacgatgtc cgtacaacac
      601 accaagctca tgatccacac cttggccatg catcaaccat tatggccaga gtgctgccgc
      661 atccggcagc gagtcttcat tgaagagcaa catgtgcctg ttgacctgga gtgggatgag
      721 acagatgaag ctgcgataca tttattggct agcctcaatg gtcaggcggt cggttgtgcc
      781 agggttatgc ccgaggggta tatcggacgt atggccgtat tgcctgcatg gcgggggaaa
      841 ggcataggtg aggcattatt gctgcaagcg atacaaatgt gccgccagtt tgatgtgtcg
      901 catgctcgct tgtctgcaca gatgcatgcg attggttttt accagcaagc tggctttgaa
      961 gtctgtagcg agccctacag tgatgccgat atcttgcatg tggatatgca gcttgagctg
     1021 gtataattac ggtttaagtt tttctccaac ttgcagacat aaaaggaatt ctcatgtcaa
     1081 aaatcgtaat gaaagccggt gaagcgaccg tattttccga aggcaaagat gttaccgccg
     1141 cgatgccgga aatcgtgatt ggctccgtgg atggcccagt aggtactgcg tttgccaaca
     1201 tgatggcgca aaccaaaggc catacggcca tgttcgctgt gcgtgacatc aaccagatgg
     1261 tgcgtcctgc gaccatgatg gtaccaaaag tgactttgaa agactcactg aacattgagt
     1321 tgttcggtgg cgtggtacaa gcaggtgtgg ctgacggtat cacggatgcg gtgatcgaag
     1381 gtattattcc taaagagctg gtcaacgacc tgtgcatcgt tgccctgttg tggattgatc
     1441 caggttgcgc caaagaggca aacctggata aggctgacct ttacaaaaat aactacgaag
     1501 cgatcaaact ggcattgaaa cgtgcgctga atgacgaacc tagcattgat gaaatcatcg
     1561 ctaaccgtca caaaatcaag cactgtatgt gggaagacag ctgggatcaa aaataatcct
     1621 gacgttttct acctaacccc gcgattgcgg ggtttttgct atctggacta aaacgatgaa
     1681 gtatttgatg aagcgttacc tggccgtggt ctacgaggcc ttgctcatgc tggccctgac
     1741 cctggccctg acggcggcct atgtcatgct tgttggcgat gccagtcatg gctggaaaag
     1801 attgggactg caggccttgc tgtggctgac catgggagct tatttcgtgc gctgctgggt
     1861 ggtgaccgga caaacgctgg ccagccagac ctggaagctt aaggtggtgg ataggcatgg
     1921 tcagttactg agccagcagc aggccatgct gcgttatata ttggccagtc tgttgttgtt
     1981 gcctgccgga ttgacgttgt ggtgggcctt gctggaccgc gatcatcaat tcttgcatga
     2041 ccgcttattg gggtcgcgta tgattacttt gccaacagcc taacgccgtt caacatagta
     2101 aagcatcccg agcccggccg ctaaaaagag cagtgtcggg ctgatggcac tcaccagagg
     2161 agaccagtca ttcagcacac caaggtgtac aaatacgcgg ttcatgatct ggtagaccac
     2221 ccccaggaaa atacctgtaa aaatcttggc actgatgcca cctgctcgtt gttgtaaaaa
     2281 gccaaacggt aacgccagca tcaccatcac caggcaggct aaggggtaaa ccagcttgga
     2341 ccataacgca acttggtaac gggtgctgcg ctgcccgttt tggtgcaggt actggatgaa
     2401 gctatacagg ttccaggcag acatcttttc cggcaacacc agcaatacat tgagcaattc
     2461 cggcctgatc agcgattccc acttggtttg tttgaaaaat tgggaggtca ccgaattgtt
     2521 ttgtatggcc ttgctggtat tcattttggt ttgtgtcaca ttcgataaca cccaatgatc
     2581 atcctcaaag taacccttgt cagcatggct gatcgtacgt aagcggaatt gtttatcaaa
     2641 gtcataaata cggatatcta ccaggctggt gtctggcatc acagtgttga cgttgacgaa
     2701 gctgctgcca tccttgatcc agagaccaga ctggaaatcc tgggcaatga cagagtctgt
     2761 ggcctggata cggatacgct gtgccatttt ttcgctaatc ggcgcaatga tttcaccaat
     2821 taaaaaggtc accagtgcaa agacaaggcc gatgctcaac agcattttgg caatcgtttg
     2881 cagtgaaatg ccactggtac gcatgacaat cagttcagag ctgctggcca tggcgcccag
     2941 tgtatacaca atgcctagca gaacggcgac cggagccact tcgtaaatat gtcctggaat
     3001 acttagtgct acataaatga gcatactgtt gatgccataa tgtccacgtc ccaagctatc
     3061 aagttcttgc agcaggtcaa agaaagaaaa catggcgagc aagccgagta acaccagcat
     3121 gatgctaaag ctgatttctt tccagaaata acgattgaga atgactagcg tcatgatgat
     3181 gtgcgtttag cccgacgctt gaaccagtcg ggaatcaatg gtttgagatg tttgcggcgg
     3241 taatagaggt accagcctag caagataaaa ctgaaatgta ctggccataa gccgaccatc
     3301 accgagattt tgccctgagt gatccaagcc tgaaagatgc tcagcatatt gttgtatatg
     3361 atgaagatca ggatggcaaa cataatgttg agcgaacgcc ctgcacgcgg gtcgacaaag
     3421 ctcagtggga tggcaagaag aaccatcaca atcgttgaaa tcgggatggc caatcgccaa
     3481 tgcaactcag catggtcggc ggctgttgcg cccgcgagta atgactgcgt tgatttggtt
     3541 tgtgcgctac tagtcgtagg cgctgtttct ttggtttcca cccgtatgtc gtaacgctcg
     3601 aattcagtgg tagtgacttc cagggtgttg gggatggttt cataacgcct gccatgctcc
     3661 atgcgcagaa agttatcgcc attgctggct ttatagcggc taccgctatt ggcggtgatg
     3721 atgccggttt taccattctg cttggactgc acaaagatgt ttttgacctc gtaacccagt
     3781 ttgtcgaagc tttcgataaa gtagatgcgt tcgccgttat tggactcttt aaaggtaccg
     3841 gggctgatgg aggagagttc gtcgcgtttt ttgagttgtg cgcggtagtt gtcagccttg
     3901 tcgttagccc agggcataat caataggctg aacaggctga tgatcagtac gatgggtgta
     3961 atgaagcgca agatgggtac cacccactgc cgcaagctga gtcctgcatt catccagatc
     4021 accatttctg aatcacggtg ccagcgcgtc aacgtcaata agacggcgag gaaaatcgcc
     4081 agggatagca tcatgggcat aaattgcacc aggctgaagg tcaggatggt gccgatagca
     4141 tcgttaggca ggatgcccct ggatacctgg cgcacgatat tgccggcacg ctgtgccacc
     4201 acaatgccaa ggataatcag gaaaatggca gaggctgagg acatcagctc cgcagacaat
     4261 gcacgtttga aaattttcaa tttagatgca ccgctttgaa atcagcataa aacactagat
     4321 aatacaacca tcactcaggc aaattgcatg gaatcacaaa ggataaagat ggaatttagc
     4381 acaaaaagcg ctgccccgga aaaattgcgt acggattgct tgctggtcgg catttatgaa
     4441 ggcaaaaaac tgacggattt agccaaggca ttggatacgg tcagtgaaaa ggcgatttca
     4501 aatgcgctta aatccggcga tatggaaggc aaggcaggca ccacgctgat attgcggcag
     4561 gttgcgggtg tggtggcttc cagagtcatg ttagttggtc tggggaaagc ggacgaagcg
     4621 agcgttaaga gtttgcgtaa tagttttcgc gtagctttaa aagccttacc cgtcggcgta
     4681 gaaagcgtag cgactgactt tgcaagtctg gggatcaaaa agatcacgac gcaacaaaaa
     4741 gcggctgcac tggcggaagt gaccttggat gcaacataca aggttaacgc ggtaaaagcc
     4801 aagtctgtgg aaccacatcc gctcaagcaa gtggtcttgt tggcagacaa ggcagaaatt
     4861 gccgctgttg aggttggtgc caaacagggt caaggacttg ggcagggggt tgcgctggca
     4921 aaagacctcg gcaatttgcc acccaatatt tgtaccccga gttacctggg taagcaggcg
     4981 gagaagctgg ctaaagagta tggctttaag gtcgaagtgt tggaccagcc tgcgattgag
     5041 aaactcaata tgggttcttt cctgggcgtg acacgtggca gcgaagagcc accacgcttt
     5101 atcgtactcc agcatctgca gggtaaaaaa tcgcagaaac cggtggtgtt ggtgggtaaa
     5161 gggatcacgt ttgatactgg cggtatttcg ctcaagcccg gtgccgacat ggacgaaatg
     5221 aaatatgaca tgtgcggtgc agcttcggtg ctcggtgttt ttaaagcgat tggcgagtta
     5281 aaactgccat ttaatgtggt tggtttgatc cctacctgtg aaaacatgcc aagcgggcgt
     5341 gcgaccaagc ctggcgatat cctgaccagt atgtcaggtc aaaccattga ggtcttgaac
     5401 actgatgctg aaggtcgctt gatattgtgc gatgcattga cctatgccga acggtttgag
     5461 cctgctgccg tgattgatat tgccacctta actggtgcct gtgtcattgc gctgggacat
     5521 cacgtcagtg gcttgtttgc caataatgat ggcctggcta aagctttatt ggccgctggt
     5581 gagcaagcca atgacagagc ttggcagatg ccattatggg atgattacca gggccagctg
     5641 gatagcaatt ttgctgacat ggccaacatc ggcggccgtg cggccggcag tattactgct
     5701 gcctgcttcc tctcccgctt tgccaaaaaa tatgactggg cgcacctgga tatcgcaggt
     5761 accgcctgga agtccggtaa ggaaaaaggc ggtactggtc gtccagttcc cttgctggtg
     5821 acatatttgc tggagcaggc agccaagtaa atgtgatgac taggatccgt ttttatactg
     5881 atgtgactga caagattgca ttgatacagc atctggtcag ccaggcgctg tcgcggcagc
     5941 ggcatgtgac tgtgtatgta caggaccgtg aacaggccgt gcagctcagt aatggtttgt
     6001 ggcaacaagc ggcagaaagc tttttcccga atgcattagc cgatgccgga catgttgcgt
     6061 tgacgccagt gcaactcgtc tggcaaccgg agcaagtaaa ccaggatgac cttttgttta
     6121 attgccaggc aagcctgcct aagtttttca gtcgcttccg gcatgtgttt gagttggtgg
     6181 gtactgatga gagtgataaa gccgcggcca ggcagcgttg ggcgttttac cgtgatcgcg
     6241 gctatgaaat ccagcatatg aataagcaag tgtagtcaac agcattgcaa gggagatggt
     6301 gtggatattt cagaaattcc tgtactaacc aaagtcgtgc acaaatcggc ggtcccagcc
     6361 accgattttg atgaactggt agcacagctc aaagaagctt tgctacctga agtcgcgcaa
     6421 cttgtctctt cacagctggc caagcaccag ccagacgcga cgacaggaca gcagtcactg
     6481 gctgagtatg cgaacacctt gcagcaggag ttgatggcaa aagcacagtc gcagatgggc
     6541 gacacgattc aatctgtaga gcaggccttc actgaagcca tgggcaatgt cagcaaacaa
     6601 caactgcaga catttcaagc gacgctggaa agtcagtcaa ctcagcaatt gcaggcaatt
     6661 gaagaaaaac tctcgcattt aacggagata cagcagcaat tagtgtcttc agccgagcag
     6721 tctttgcgcg aaacattgga aaagaccacg gagcaatcgt taaacgatgc ccttatgcct
     6781 ttgattgagc gtcagcaagc gcagttagaa gggcgtgtgc aagtattgcg tgaacaatta
     6841 gagcaggggc tggatgtcca tttacagcaa ctgcaggaaa atagtcgcca aactttggct
     6901 gcgggccagg aagttgctgc gacagagttg gcggcagatt tcaaagtgtc tttgcagcag
     6961 gcctttgccg aattgaatgc ctcacaactc agtgagttca ggcaaaacat gcaatctgaa
     7021 ctactagcat ctgaaactgc cttacaggaa aaagtggcgg caatggtggg tacgcaattg
     7081 caggagatgg aagccgaaat gaacaagcgg cttaaatccc gtatcctcga agtattgcag
     7141 ggcatcaaat tcgtgatgcc gacaatttag attaggcgtt tgctagagtc agtaccacgg
     7201 gggtgtggtc gctggggcgc tccagtttac gtggagtctt gtctatcaca cagctctggc
     7261 ataacgcctt taaggcgtcg ctgaccagga tatggtcaat ccgcaatccc atattgcggc
     7321 ggaaagccgc catccggtag tcccaccagc tgaactgccc agcctcttcg ttaaatagcc
     7381 taaaactatc gtgcaatccc aacgccaata attgttgaaa cgcttcccgt tccggtgcac
     7441 tcacaagaac ttgcccttgc caggcggcag ggtcgtggca gtccctgtca tccggtgcaa
     7501 tgttgtaatc gcctaacaat gccagtttac tgtgttgact gagttctgct tttacccagg
     7561 catgcaagac gcctaaccaa ttcagcttgt actgatattt atcggagtct atggattgtc
     7621 cgttgaccac ataagcgcaa atcacacgta catcctggat cgtcgcggca ataatccttt
     7681 tttgcgtatc ggcaaaatca ggcaggtcca gatgtacgga ttctatggca tgcgggctga
     7741 tgatagcgac cccattatag gttttttgtc cgttatgcac tgcgttgtaa ccagcttctt
     7801 taagtgccgc gtagggaaat ttgctgtctt cctgtttggt ttcctgcagg catagcacat
     7861 ctggctggtt ggccgccagc cagtctaata cgtgcggcaa gcgcacagtc agggagttga
     7921 cattccaggt agccagtttc aacagtattc tccaaaacta ttttttcaag gtgtttgtga
     7981 gacacccgct tttaggcgca ttttacatga ctacgtagcg caagctgaaa catccagaca
     8041 gtaatggtcg ggttttccgg gatatcaggc aaatagcgta tttctaaatg atgttatcgc
     8101 ctctacagtc ttgcctgata agttcatgtc aggagggcag catgtcgcgc cgtacagcaa
     8161 gtttttcaag agcagagtgg tggctagtcg tggctgtcgt gctggtagcc atcagtttgc
     8221 gtcctggaat tgtgtctatg gggccagctt taactgccat catagaaaat ttccggttaa
     8281 gccatgccac tgcctcaatg atgacggcga tcccggatgt cttgatgggc gcgctcgcct
     8341 tgcctacgcc ttggcttgtg cgccgctttg gggtgaaccg gattctgatg ttagccttgg
     8401 cattattgag cctgtcgatt gccgtccgcg cttatgccgg aaccagtgct gtacttatca
     8461 tagcgacggt tggtgttggc gcaggcattg cggtcgctgg cgccatgttt gccgggattg
     8521 ccaaggccag ttttgccaat catgctgctt tcatcatggg tatctatgcc accgcattgt
     8581 ccttgggtag caccttgtcg gccgggttga ctggctggat gctgcaattg gcgggatggc
     8641 gcttggcaac cggtatctgg gctgtgtttg gcttgattgc tatcgttgcc tgggggctgg
     8701 tgatacggaa gtcagtcacc accaggcagg cttctgttat atcttctaca gccaccgccc
     8761 cgtcccgatt gcctttgcat acgccagtgg cctggctgat tgcgttattc tttgcctgtg
     8821 tcaatttcct gttttactcg attttgactt ggacagctgc catgtatcag gaaaggggaa
     8881 tgccggctgc tgaggcaggc gtcgtcctgg cgtgctttac cttgtgtttt atgcttgcca
     8941 gcccggtggt agggatgtat agccggcatg tggataggcg tggctggctt gtgagttgcg
     9001 cattattgtc aaccataggg ttggccggga tggcatcgcc aggcgcgtca ccctggttat
     9061 gggtgccatt aacagccatg ggtttgggtg gtgcgtttac gctggccatg accttgccgc
     9121 tggatcatac gcacagtccg gatgcagcca atcgctggaa tgcttttgtg ttgaccattg
     9181 gctacctgat tgctgccacc ggtcctttta ccatgggctg gatgcgggat gtcagcggcc
     9241 agtttgaggg cgcgtatcag ttactggttg gggttgccgt actcatgctc gtgatcgcca
     9301 ccaggttaaa gccaccctcc acttaagcat ggcatcgttc aattgctttg ataccatcat
     9361 aaattcctgt ttgtttggat aatgcgttgg atttggtttg cggtataaat taggctggat
     9421 ttgattgtcg gcagattgcc cagtcaagaa ttgtactaat gttgcccgga cgcgcatgaa
     9481 gcctgtttcg cccctatggt tactcagatg gtcactgacg atagtcatgc tggccgtctc
     9541 cgtgctatcc agcgcctccg aagagatcac cctgcaactg aaatggcgtc accagttcca
     9601 gttcgcaggc tattatgccg cagtggcgca gggctattat cgcgaagaag gcttgaatgt
     9661 caggcttgtg gagggtagca aagacctgcc tccgttgaag gaagtgctgt caggacgcgc
     9721 tgagtacggc attggcgatg ctgagatcct agtgtcgcgc atttctggta aacctgtagt
     9781 cgccttggcc gccattttcc agcattctcc cggtgtattg cttagcctca aacagagcaa
     9841 cattcaaaag cctgaggact tgattggtaa gcgcatcatg ctgtctggtg acggtcaggg
     9901 tgtgtatcaa ttcaaggcca tgttattgaa acagcatctg gatatcagcc agatgactat
     9961 tgtgccgcat acctggcgcc tgcaagacct gattgaaggt aaagtggatg tagtctcggc
    10021 atatgccatg gatgaaccag gacagcttca gcaaatgggt tacgaaacat ctatgatcag
    10081 taatcacccc tatggggtcg atttctatgg cgatgtgttg ttcaccagtg agcgtgaaat
    10141 tgcgatgcac ccagagcggg taagggcttt tttgcgtgcg accaaaaaag gctggctgta
    10201 tgcacttagc caccaggaag aaatggctgc gctgatcgca gggatgcctg gcgtgactca
    10261 acgtggcctc agcaagcaaa cgttactgca cgaagccaat gtgatggtgc cttatgtact
    10321 taccgatgtg gtgcccttag ggcaaatgca cgaagaacgt tggaacacta ttgcacaaat
    10381 gctggcggat ttgggattag tctcccagca ctatgacctg aacgggttta tgtaccaggg
    10441 tgacatgtca ggcaaacgac cttacatggt atggctggta cgtagtctat ccatcctgtt
    10501 actggtattg ggggccacct ttgtctggaa tgtccagata cgacggcagg tcattcaacg
    10561 tacccaggcc ttgcaaaacg aaattgatcg acgtacgcag gccgaatact tacttaaagt
    10621 cgccggtaac gtggcaaaaa ttggtggctg ggtgatagac ctgaaaacca atctcatcca
    10681 ttggtcagac gaagtggccg ctttacatga gatgccgccg ggatattctc ctacagtcga
    10741 agagggcatg aacatgtttg tgccggagtc ccagcctgcc atggcggggg cgctcaaggc
    10801 ttgcagagaa gcaggcgtgc cttatgatct ggaactggag aaaataaccg cgaatggccg
    10861 ccggttctgg gtacgggcaa tggggcagcc tgtctatgat caggcgggca acgtgatcaa
    10921 gctacaaggc tccttgcagg atattaccgc ccacaagcag ctggaagcca tcaaacaagg
    10981 gcaggataga atccaggcca tgatgttggc agatattgcc ttgccagata tcctgcatgc
    11041 tgcggtgcag ctgattgagc agcatctccc agatgcgtat tgtgcgatgt tgctgctgga
    11101 tcaggatggt tcgtacttaa gacacaccgc ttcatccaga ttgccagccg actatgcgaa
    11161 agcgattgat ggcataccat tgggtacttg tgggatcgcc gcactgaccg aaggccgggt
    11221 gattgctgag gatgtgactc agcatccgct ttgggaaaag tgctgtgaac tggcaatgac
    11281 gcatggctta cgtgcatgtt ggagcatgcc ggtgttgtct cgcagtcgcc gagtattggg
    11341 cgtattggcc atctaccgca agcaacgtca tacgccagga actgccgagc tcgactttat
    11401 cgacagttat gcccaaattc tcgggttggc gatcgagcgt gagcaagcgg atgaacaatt
    11461 acacctgctg caaagcggta tatccaggct caatgatgtg gtcatgatta ccgaagcgcc
    11521 catgagtacg cccatgcagc aaaaaatcgt gttcctcaac gatgctttta cccggattac
    11581 tgggatgcct gttgtgcaat tgcagggcat ctcaccattt gagattttca aggacgcgac
    11641 accagggcga gagctggagc gtatcaaaca tgctttcctg catatgcagc cgatcaaaac
    11701 cgaggtggtg gccattaatc aggaaggcgt cggcatttcc cttgaaatgg atgcgctgcc
    11761 tttgcatgac aaaggcggca ggatgacgca ttgggtggct gtgttgcggg acattaccga
    11821 gcgtaagcag gctgatgcgc agatccgcca actggcttat tacgacacca tgaccggtct
    11881 gcctaaccga ctgttgctgc aagacaaact gtcttcgcat gtgcgcaagg caatgcgcaa
    11941 acaagtcggg ggcgccttat tatttattga tatcgacaac ttcaaaacgc tcaacgatac
    12001 ctatggccat gatgtcggtg atgccttgct ggttgaagtg gcccggcgca tcaggcagtc
    12061 tgttcgccga ttggatactg tcagccggct gggtggtgat gaatttgtgg tggtgctgga
    12121 tagcttgcat tacgagccgg cacaggcaga aaagcaggcg cgccgagtct gcgaaaaaat
    12181 catggcggcc tttaaaaaac cattcatgct gaagcattac cagcattaca ccacacccag
    12241 tattggcgtg gtgttattcg atcctgagca cttgagccag acggacgaat tgttacgtcg
    12301 ggcagatttg gcaatgtaca aggccaaaga agcaggcaga aatgggttcc gcttttttga
    12361 tacccaaatg gaaacggagt tgcgtcaccg ggtggcactt gaagctgact tgcatgctgc
    12421 cattgagaaa caagagttca cgttgcattt tcagccgcaa tacaaccata accacgagat
    12481 tgtgggtgct gaagcactgg tgcgctggca gcacccgcaa catgggctga tcatgccggg
    12541 gcaatttatt ggcctggctg aaagttctgg ccaaattggt gcactggggg gctgggtgct
    12601 gcaggaggcc tgcaagatgc tacagcactg ggcaaatcat ccgcagttga aacacctggt
    12661 gctctcggtt aatgtgagtc ccaagcagta tttgcagcca aactttgtcg cagagataca
    12721 gcaaatgctg gacgaaagcg gggtttcacc tgggcatctc aaacttgaac tgacagaaag
    12781 catgctggtc gataatgttg aagatatcct cgccaagatg gatgctctca ggcagattgg
    12841 catttgtttc tccctggatg acttcggtac cggctactct tctttgtctt acctgaagcg
    12901 ttttccgttt gaccagttga aaattgacca gtcttttgtc cgcgacatgt taaatgggcg
    12961 tgaccatgag agtattgtga acgccattat ttcattgggg caaagcctac agttagaaat
    13021 tctggcagaa ggtgtcgaaa ccgaagcaca gttaagtgtg ctgaaagacc tcggttgcga
    13081 aatattccaa ggttattatt tcacccggcc attgtcactg gaggcattcg agacgtgggt
    13141 gattcagcca gatcaagcag tcatacacac aagtacagca caggccgtat aagtccaatg
    13201 gcagaaaaac atctgttggc acccgctacc tgcctgttta gtctgcttta ttgatacagt
    13261 acaaataccc cctcgaagat ggatgtcgtt ttggcctggc agacgacacg tgcactcagc
    13321 gtcatccttg cgcgaccatg ctgatgaaag ttatctacaa attgggacca tgcatctgct
    13381 gaagacatcg tcgcaataca ctcaaaatcg gtatgaacgg gcaacaggaa tttcacttcc
    13441 tgttgcttga tcaccagctt gcccttgagc cctgcctgct gtatgttgtg ttgtagtagt
    13501 gaccaggcag tcgtaatcga aatcgcagca atactgccac caaatacaat atccagatgg
    13561 tttttgttct gtgcatagga accttgcaca atcagttcag atgcgtttat ttttacgatc
    13621 ttgatcccaa aattcctggt ttggggaatc agctcaaaca aggtttgttc gaaagtggat
    13681 tctagcgaag tcatggatat tccttaagtc acatatag
//
LOCUS       DATMSK010000132         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_173689,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000132 DATMSK010000000
VERSION     DATMSK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173689"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VNZ84_04515"
     CDS             complement(<1..233)
                     /locus_tag="VNZ84_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HWU66251.1"
                     /translation="MSFQQGLSGLNAASKSLDVIGNNVANANTVGFKQSRAEFADVYA
                     NALNGSGTGSAGIGVKVADIQRQFTQGNITNTE"
     gene            complement(269..925)
                     /locus_tag="VNZ84_04520"
     CDS             complement(269..925)
                     /locus_tag="VNZ84_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HWU66252.1"
                     /translation="MTTISNDLLTTMNTRKSGSGSTAADAQDRFMTLLITQMKSQDPL
                     NPMDNAQVTSQLAQLSTVTGIDKLNTTMESMISNVQSSQTYQASNMIGRYVLTKGDSL
                     TLTESKSYLGINLPVGADKVTVTIKDSAGNQMRQLTLGKQEAGVLPLSWDGYKDDGSK
                     AADGNYKFEVTATTANTSVTATPLSYDQVMSVSNSSSGIKLNLGKLDSIGIDDVVEVF
                     "
     gene            complement(946..1350)
                     /gene="flgC"
                     /locus_tag="VNZ84_04525"
     CDS             complement(946..1350)
                     /gene="flgC"
                     /locus_tag="VNZ84_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HWU66253.1"
                     /translation="MSLFNVFNVSGSAMSAQSQRLNAVASNLANADSATSANGQPYKA
                     KQVVFSATPTNNAAVSGVKVQQVIEDPSPPKVVYDPKHPAANAQGYVTMPNVNVTEEM
                     VNMISASRAYQNNVETMNAAKTMLLKTLTIGQ"
     gene            complement(1376..1804)
                     /gene="flgB"
                     /locus_tag="VNZ84_04530"
     CDS             complement(1376..1804)
                     /gene="flgB"
                     /locus_tag="VNZ84_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HWU66254.1"
                     /translation="MNRLDAALAFQQSALRVRAQRQELIAANIANADTPNYKARDINF
                     NDALNNALSGNDTPGSGLDVSKTSSAHLSGDPTSVDYVPTGKPGEPMFRPLIQGSVDG
                     NTVDMDVERNQFTDNAIRYEASLNMINGQIKKLLSAIQGQ"
     gene            2057..2785
                     /gene="flgA"
                     /locus_tag="VNZ84_04535"
     CDS             2057..2785
                     /gene="flgA"
                     /locus_tag="VNZ84_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HWU66255.1"
                     /translation="MASHSIVKQFLAFCTGILLLAGSALSHAEAMPNTDQTQNLTSIR
                     KKVEEFLTVQTAGYPGRVTVTAGAIDNNLKLAKCPALEVFMPQGSRAWGKTNVGVRCN
                     APAWTIYMQATVNVVGQYLVAAAPLAQGRVVDSGDLLFESGDLAQLPPGIFTDPEQAI
                     GRIVNISMNAGTVLRQEMLKASPVVQQGQTVTLITRGKGFSISAEGHALNNATDGQLV
                     RVKVANGQVVSGIARNGGKIEVSF"
     gene            2930..3241
                     /gene="flgM"
                     /locus_tag="VNZ84_04540"
     CDS             2930..3241
                     /gene="flgM"
                     /locus_tag="VNZ84_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HWU66256.1"
                     /translation="MKINDSIQKTTGLVTEKIESGTAKKTPAPAVGGATATESVTLSP
                     LSSQLQSLEARIATQTVYDTDKVDAIKTAITNGQFQVDSGKVADGLIETVKDLLTSAK
                     S"
     gene            3249..3707
                     /locus_tag="VNZ84_04545"
     CDS             3249..3707
                     /locus_tag="VNZ84_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HWU66257.1"
                     /translation="MSTSISFEQDSQLVNQLIVLLSREQASLVRANMDEIEQILDEKA
                     HLLSELGSTVQKRHDALVKLGFEGSEKGMADWLVAQKTVAVQQSWMQFHKMLVQAQEM
                     NRVNGVLINKHFSRNEQFLNALHAHHSPGQMYGANGQATTQSYLRSGLQA"
BASE COUNT          816 a          949 c         1088 g          899 t
ORIGIN      
        1 ttttcggtgt tggtgatatt gccctgggtg aactggcgct ggatgtcggc gactttcacg
       61 ccgataccgg cggagccggt gccagaaccg ttcagtgcgt tggcgtacac atcagcaaac
      121 tcggcgcgcg attgcttgaa cccgactgtg ttggcgttgg cgacgttatt gccaatgacg
      181 tccagactct tggatgcggc attcaggccg cttaaccctt gttgaaaact catgttgatc
      241 tccttgttag cgtgaggacg tgcattgact aaaagacttc cacgacatca tcaataccga
      301 tggagtcgag tttgcccagg ttgagtttga tgccactgct gctgttggat acgctcatga
      361 cctgatcgta agacaacggc gtggcggtga cgctggtatt ggcggtggtg gcggtgactt
      421 cgaacttgta attgccgtct gctgccttgg aaccgtcatc cttgtaacca tcccagctca
      481 gtggcaatac gcctgcttcc tgcttgccca gggtcagttg gcgcatctga ttgccggcag
      541 aatccttgat ggtgacggtg accttgtcgg cgcctaccgg caggttgatg cccagatagg
      601 atttgctctc ggtgagggtc aggctgtcgc ccttggtcag cacatagcgg ccgatcatgt
      661 tggatgcctg ataggtctgg ctggactgca cgttgctgat catggattcc atggtggtgt
      721 tcagcttgtc gataccggta accgtggaaa gctgggccag ctgactggtc acctgcgcgt
      781 tgtccatcgg gttgagcggg tcctggcttt tcatctgggt gatgagcagg gtcatgaacc
      841 ggtcctgcgc atcggcggct gtggagcccg agccggattt gcgcgtgttc atcgtggtca
      901 gcaggtcatt cgaaatggtc gtcatggcgt gatccttaaa ggaaattatt gtccgatggt
      961 cagtgttttc aacaacatgg tcttggcggc attcatggtt tccacgttat tctggtaggc
     1021 acgcgaggcc gagatcatgt tgaccatttc ttcggtcaca ttcacgttgg gcatggtgac
     1081 atagccttgc gcattggctg ccgggtgctt ggggtcgtac accaccttgg gtggagatgg
     1141 gtcttcaatc acctgttgca ccttgacgcc gctgacagcg gcgttgttgg tgggggtggc
     1201 gctgaatacc acctgcttgg ccttgtacgg ctgaccattg gcactggtgg cgctatcggc
     1261 gttggccaga ttgctggcaa cggcattaag ccgctgcgac tgggcgctca tggctgagcc
     1321 ggacacattg aatacattga acagagacat gatggtgctc ctttatataa tccggctatt
     1381 ggccctgaat cgccgacagc agcttcttga tctggccgtt gatcatgttg aggctggctt
     1441 cgtaacggat ggcattgtcg gtaaactggt tgcgctccac atccatgtcg acggtattgc
     1501 catccacgct accctggatc aggggacgga acatcggttc gcccggcttg ccggtcggta
     1561 catagtcgac actggtgggg tcgccactga ggtgggcgct ggatgttttg ctgacgtcga
     1621 ggccagagcc gggggtgtcg ttaccgctca gggcattatt gagcgcatca ttgaaattga
     1681 tgtcgcgcgc cttgtagttc ggggtatcgg cattggcgat attggcggca ataagctcct
     1741 gcctttgcgc acgcacgcga agagcggact gctgaaatgc cagtgctgca tctagtctgt
     1801 tcatgtgtac tgacctcctg tttcgatccg gcctaaagtt ttcacccagc tcaggttttt
     1861 acctggctca agttttcacc tggctcaagt tttaacttag tgcagccgtt atctatgatg
     1921 agggcgagtg tagagcgcga cagtcaactt caatcagagg agtaaagggg gaaatgtcgt
     1981 ctttttccac catttacttt tgtcccggct acgtagaatg caggctgtat ctttctccgc
     2041 agtcgaggaa cgaatcatgg caagtcattc tatcgtaaag caatttttgg cattctgcac
     2101 aggaatattg ctcctggctg gcagtgcgct ttcccacgct gaagcgatgc cgaatactga
     2161 ccagacgcag aacctgacct ccatccgcaa gaaggtcgaa gaattcctga ccgtgcagac
     2221 cgccggttat cccggtcgtg tcaccgtcac tgccggtgcc atagacaata atctcaagct
     2281 cgccaaatgc ccggcgctgg aagtgttcat gccgcagggc agccgtgcct ggggcaagac
     2341 caatgtcggt gtgcgctgca atgcgcctgc ctggaccatc tacatgcagg ccacagtgaa
     2401 tgtggtgggg cagtatctgg tggcggccgc gccgctggct cagggccgtg tggtggatag
     2461 cggtgacctg ctgtttgaaa gtggcgatct cgcccaactg cctcccggta tctttaccga
     2521 tcccgaacaa gccattggcc gcatcgttaa tatttccatg aatgccggta ccgtgctgcg
     2581 ccaggagatg ctgaaagcgt cacctgtcgt acagcaaggc caaacggtga cgctgattac
     2641 gcgaggcaag ggcttcagta tcagcgccga aggccacgca ctcaataatg ctaccgatgg
     2701 ccagctggtg agggtcaagg tcgccaatgg gcaggtagta agcgggattg cccgcaatgg
     2761 tggaaaaatt gaagtcagtt tttaagcatg tgtttttgcg tcgaatgaat gtaaaggctg
     2821 gcaagagccg tttcaaactt gccgatcggt gttaaagttt aagaaaaccc tgccgatatg
     2881 agccttatag aaggcaagat gcacacacat tcatgcatga ggaaaaacca tgaagatcaa
     2941 tgactccatc cagaaaacca ctgggttggt gaccgagaag atcgaatccg gcaccgcaaa
     3001 gaaaacaccg gcaccggcag tgggtggcgc aaccgccacc gaaagcgtca cgctttcccc
     3061 actgtcctcg caattgcaat ccctggaagc acggattgcg acgcagacgg tatatgacac
     3121 agataaggta gacgccatca aaacggcgat taccaacggc caattccagg tcgattccgg
     3181 caaggttgcc gatggcctga ttgaaaccgt taaggattta ttaacttcag ccaaatcgtg
     3241 agataaccat gagcacaagc atctcgttcg agcaggatag ccaactggtc aatcaattga
     3301 tcgttttatt atcccgtgaa caagccagcc tggtgagagc caatatggac gagatcgagc
     3361 agattcttga tgagaaggcg catctgctca gcgagcttgg cagcacggta caaaagcgtc
     3421 acgacgctct ggtgaaactg ggcttcgaag gcagcgaaaa aggcatggca gattggctgg
     3481 ttgcccagaa aaccgtggct gtgcagcaat cgtggatgca gtttcacaaa atgctggtgc
     3541 aagcccagga aatgaaccgt gtgaatggcg tgctgatcaa caagcacttc agtcgtaatg
     3601 aacagtttct caatgcgctg catgcgcatc actcacctgg ccagatgtat ggtgccaacg
     3661 gccaggccac gacgcaaagt tatctgcgga gtggattaca ggcctagcga acttccagtt
     3721 cctccagagg cgttaagtgt agttaacgcc cc
//
LOCUS       DATMSK010000133        15290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_127080,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000133 DATMSK010000000
VERSION     DATMSK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 15290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15290
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127080"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1794
                     /gene="mshL"
                     /locus_tag="VNZ84_04550"
     CDS             22..1794
                     /gene="mshL"
                     /locus_tag="VNZ84_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HWU66258.1"
                     /translation="MKTFLNTLGVMVIGAGLTGCTSNPFAPPNSSINPKIQNELATSA
                     AKAQAQVTPPPKEVTDELFEPLKIEPPKALAKRVEPRFDLVVNKAPAAQVLMGIVSGS
                     PYSIALSPELKGEISINLRDVTLFEALNALRDLYGYEYKMNGKQIFVEPQTLQTRVFQ
                     VNYIMGARRGTSATRVTSGTASGSGAGGAAGVAGQAAAMNISGSGSGTGTGTATGNGT
                     SGASNLQVYASDVSMRTNNDFWDEIAGSLSGLIGEENGRKVIINAQSGLIMVKAMPKE
                     IRQVEKFLSLMQVTVDRQVILEAKIIKVQLNRNSQQGINWAFLRKGARDVTFGNVNGN
                     TSLGTTGTITDGTVSSTAPGFLSNSSPIPLGGSAFALAVQGGNFSALLTFLETQGNVE
                     VLSSPRIATINNQKAVLKVGNDAFYVTNVKTNVTTTTGGAIVTPDIQLQPYFSGISLD
                     VTPQIDKDDNIILHVHPFVSDVTQVNRTVTLSSANGGTYTIPTASSNINETDSIVRTR
                     DGSVIAIGGLMSESVDNTRTKVPGLGDVKFLGNLFRQKDLSSTKTELVILLKSTVVRS
                     GENWAQDMLESQDRVDKLKADTAF"
     gene            1803..2693
                     /locus_tag="VNZ84_04555"
     CDS             1803..2693
                     /locus_tag="VNZ84_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU66259.1"
                     /translation="MYLQHFGLKEYPFTITPDTSFYYATRSLQEALNTLLIAIQSGEG
                     FVKITGEVGTGKTLLCRRLLKALASNCKIAFIPNPYLDPHALLMTLATEFGVAFKPDF
                     THHDMIDALNRKLLTFAEEGQHVVVCLDEVQAMPLETLEALRLLSNLETEKRKLIQVV
                     IFGQPELEERINHPSIRQLRQRIGFEYHLKGLQWSELGFYLNHRMHVAGYQGGMVFTP
                     ASMRLLYRYANGIPRLLNILAHKSLLSAFGRGQQGVSAEDVKAAILDTQASVKAQHRW
                     LGHGFKWWLAFHTAMAGWGR"
     gene            2648..3937
                     /locus_tag="VNZ84_04560"
     CDS             2648..3937
                     /locus_tag="VNZ84_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWU66260.1"
                     /translation="MVAGIPHGYGRLGPLVEVLGKPLSLINQVLQNVEARQGGVAMDG
                     KPDAAGWSAQVKPVLSHQGGHSGRWLRRLAWGLVLLTLAGWLLMNRPMLMAYFQPSPF
                     AATQNKGIATQVEAPSAAAKPEVEAVAIPAPAISSTPQLTRSLFSAWQSESPAAVSPV
                     APRHQPVANAAPAKAVPAPQVEGEFSIKPAEATAANPVVSVIEPPLEAANTAKQVKSN
                     QEEAVVRGVVNKQVRPDQEVNLLIQRAVDHEQKGRLNEALVTLRQAINSYPQSEDARQ
                     LLAAYLFESKQDSEAVAVLQTGIKQYPGQIGLSKSLAKWQLSHGQPEPVLQTLNPVAN
                     LLIQDAESQWMLAMAYQQTGQHAAALPHFERAMVLRPGVAQWMVAYAISLQAAGQSAQ
                     ALQQLQLAYNMPLSERLSEFVSQRIRQLGGTVPARNE"
     gene            complement(3989..4438)
                     /locus_tag="VNZ84_04565"
     CDS             complement(3989..4438)
                     /locus_tag="VNZ84_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWU66261.1"
                     /translation="MHQFKYHQQLSLGHLLATSLLDRLPTFPGPEQPHILMAMPMHPN
                     RVRERGFNHALELAKQVHRHWQIPLEIHGCTRVIDTPTQAGMDMKTRTRSLRGAFTTQ
                     SQWHGKHVVVVDDVMTTGASMHAVAKVLKNAGAKRVTALVLARTLKS"
     gene            complement(4517..4738)
                     /locus_tag="VNZ84_04570"
                     /pseudo
     CDS             complement(4517..4738)
                     /locus_tag="VNZ84_04570"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
     gene            4841..5833
                     /gene="bioB"
                     /locus_tag="VNZ84_04575"
     CDS             4841..5833
                     /gene="bioB"
                     /locus_tag="VNZ84_04575"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWU66262.1"
                     /translation="MQTSVINLDGLKPSASCHPTVERWRVADIMALFELPFNDLLHQA
                     HTVHREHFDPNAVQVSTLLSIKTGGCSEDCGYCPQAARYHTEVENEPLMQLDEVLAAA
                     QAAKDSGASRFCMGAAWRGPKQRDLEPVLKMISEVKAMGLQTCATLGMLKDGQAEQLK
                     EAGLDYYNHNLDTAPEFYGDVITTRTYQDRLDTLDRVRSVDINVCSGGIIGMGETRTQ
                     RAGLIAQLANMEQPPESVPINLLTQVEGTPLHGTDPLDPLEFVRTIAIARITMPTSFV
                     RLSAGRQGMGEAVQALCFHAGANSIFYGEKLLTTGNPEVEADNALFSKLGLKKI"
     gene            5833..7017
                     /gene="bioF"
                     /locus_tag="VNZ84_04580"
     CDS             5833..7017
                     /gene="bioF"
                     /locus_tag="VNZ84_04580"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWU66263.1"
                     /translation="MSDLLESLQADLEKRQTQGLMRQRRLLDSPQAEHITANGQAFLS
                     FCSNDYLGLANDAALVSALQQAAAEAGVGSGASNLITGHHRYHDQLEQALAAFVGKPA
                     ALLFSTGYMANIGVVSALMGRDDAVFSDKLNHACLNDGAFLSRATHHRFPHNDVAALE
                     KLLQASTARHKLITADAVFSMDGDLAPLAAYLELCERYDAYLYVDDAHGFGVLGPQGR
                     GSLRHLGLQSPRLIMMGTLGKAAGVAGAFVAGEKIVIDYLIQYANSYIYTTPAPPPLS
                     AALLASVKEIEHGEARRKQLFDMIALLKSELSLTRWHLLPSETAIQPLIVGDTHEALA
                     LSQYLQVRGILVPAIRPPTVPANTSRLRISLSAAHREADVRALIAALHAAERELPVGI
                     QA"
     gene            7014..7793
                     /gene="bioH"
                     /locus_tag="VNZ84_04585"
     CDS             7014..7793
                     /gene="bioH"
                     /locus_tag="VNZ84_04585"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HWU66264.1"
                     /translation="MSCFIEIMGQGKPLVLLHGWGMNSAVWERITKRLSANYRLFLVD
                     LPGMGQSRPVYPYHLHSLAEAVAEEIPGVSSILGWSLGGLVAQQIALNQPDRIEKLIL
                     VGTNPCFVSKPDWSHGIEARHFEQFNERFAKDFNATLLNFLTLQCMHAKDARSTVRQL
                     RIAFGAKPTPTQDSLNQALDILLQTDLREEVGRLFQPTLIIHGDRDTLAPVSAAHWLA
                     MHLQKARLRVIAGAAHAPFLSHADAFCETVEAFMADESVEN"
     gene            7794..8666
                     /gene="bioC"
                     /locus_tag="VNZ84_04590"
     CDS             7794..8666
                     /gene="bioC"
                     /locus_tag="VNZ84_04590"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HWU66265.1"
                     /translation="MQDVYQIDKSRMRQSFHRAARQYDAAAILQRQVREEMLSRLEVV
                     KLEPTVILDAGCGTGHGLSALLKRFKKAQGIALDIAEGMLSRSKTLFPWYRFWQTSPR
                     FICADIEAMPIATGSVDLVWSNLAVQWCNDMDTVLQEFRRVLRPDGLLMFATLGPDTL
                     KELRAASGSEHTHVSRFIDMHDIGDALTRAGFSAPVLDVMHYTLTYDTVESVMRDLKA
                     IGAHNATAGRAKGLSGKGFLQKLRQGYDVFRRNGKLPATYEVVFVHAWTGSQPYTAPG
                     TSPVQFMPRKDAQR"
     gene            8670..9314
                     /gene="bioD"
                     /locus_tag="VNZ84_04595"
     CDS             8670..9314
                     /gene="bioD"
                     /locus_tag="VNZ84_04595"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HWU66266.1"
                     /translation="MHALPRHFFVTGTDTGVGKTTVTVRLMQQLIAQGFTVIGMKPVA
                     SGCEWLAGRWQNEDVRQLTAASNVKAPPELINPYCFDPPIAPHIAAEQAGVSIDLSVI
                     QSAYTQLTTMADVVIVEGAGGLLVPLNSKQSLADLIQLLNIPALVVVGMRLGCINHAL
                     LTAQVLKQRGIRFCGWVANSIDPQMSMPEENLQTLIESLQQAPLLEIPFQESST"
     gene            9315..10367
                     /locus_tag="VNZ84_04600"
     CDS             9315..10367
                     /locus_tag="VNZ84_04600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWU66267.1"
                     /translation="MESSLLTRIELRASVPPVTKGLIALNVLVFVVMLFGGAGFWHSP
                     NTVQLTWGANFAPATADGQWWRLGSAMFLHFGALHLGMNMLALWDGGKLVERMFGAVR
                     FLVIYLVSGVGGNLLSLVIQGNDAVSGGASGAIFGIYGALLVYVWFGRRQMQVQEFRW
                     LFWGALLFSTLTIVMGYVIPGIDNSAHIGGFVTGMVMAALFLPSSILPSRPLMITQSL
                     AAVAWMAAVVVLLTHLPTPKYRWQQEVAIQKQVQKLNQVDQRAQQKWRKIMLEGREGS
                     VSFDEMATQIDTEVATPYEESFEKLSALPEDPKLPSAALLEQAKAYALKRSEVSKAAA
                     DKLRNMPFTPGRPASE"
     gene            complement(10388..11506)
                     /locus_tag="VNZ84_04605"
     CDS             complement(10388..11506)
                     /locus_tag="VNZ84_04605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWU66268.1"
                     /translation="MKLAFLIFKYFPYGGVQRDMLRIARDCVAQGHEVTIFTGEWRGD
                     VAQDITVNLLKATGWLNHHRHQSLIRQMRQAITQGDFDRVVGFNRMPGLDVYFAADPC
                     YAERMAREPWYKKLSGRYRFFSTMENAVFGKQATTHILLLNQHDQAIFQRWYGTESKR
                     FHLIPPNIPLQRFIALPTQDYRAYVRQAFKLPEDAIVLLTVGSAFVRKGVDRAIDALS
                     SLPTPLKQRCWLLAVGEFESSSNMKAYAEKLGVADRCIEAGGRPDIPAMMLGCDLLVH
                     AARSELAGIVLIEAMTAGLPLLVTEVCGYAGYVSTAQAGEVLPEPFNQAQMNLVLRGM
                     IEQLPTQSWSEHAKAFVQEMARSQIAAPEARIIELVNA"
     gene            11537..12505
                     /gene="waaC"
                     /locus_tag="VNZ84_04610"
     CDS             11537..12505
                     /gene="waaC"
                     /locus_tag="VNZ84_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HWU66269.1"
                     /translation="MKRILIVKTTSMGDVIHALPVVEDILQHFPDAHIDWLVEESFAD
                     IPRLHPRVQQVFTVAVRRWRKQWWHCQTWREITTVKRQVGTQAYDVVLDLQGLVKSAV
                     MACCAHGPRHGYDKHSIREPLASRFYPHQYAITYRQHAVIRMRTLAALALGYAVPQDK
                     PAYGLVTDEHHSKQPAFLALHATSRDSKLWPEDHWVALGQYYAAQGLHMWLPWASANE
                     QARAERIAAQVPQALVLPKLSLQQLAAQMPQARFAVGVDTGLSHLAAALDIPVVAIYT
                     DTEPAFTGVAEGRRAPAVNLGGKGNVPTVEAVIAQLSVISVAVDQI"
     gene            12633..13169
                     /gene="grpE"
                     /locus_tag="VNZ84_04615"
     CDS             12633..13169
                     /gene="grpE"
                     /locus_tag="VNZ84_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWU66270.1"
                     /translation="MQEPNTPADVNANEAAVNEAAPDIQVEDTQLARIAELEAALEEA
                     KAQTLYVKAEGENIRRRAMDDIDKARKFALEKFSGELLAVKDSLDAALAVESADVASY
                     KNGVELTAKQLLSVFEKFNIAEINPVGEKFDPNKHQAISMVPAEGEPNTVLSVLQKGY
                     ALNDRVLRPALVTVIQAK"
     gene            13268..15190
                     /gene="dnaK"
                     /locus_tag="VNZ84_04620"
     CDS             13268..15190
                     /gene="dnaK"
                     /locus_tag="VNZ84_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWU66271.1"
                     /translation="MAKIIGIDLGTTNSCVAVMEGGKPRVIENAEGARTTPSIIAYQE
                     DGEILVGAPAKRQAVTNPKNTLFAVKRLIGRRFDEKEVQKDIDLMPYTITKADNGDAW
                     VEVRDKKQAPPQISAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGLDKQEGDRKIAVYDLGGGTFDISIIEITEIDGEHQFEV
                     LSTNGDTFLGGEDFDNRIIDFLADEFKKENGLDLRNDLLAKQRLKEAAEKAKIELSGA
                     QQTEVNLPYITADATGPKHLVVKITRAKLESLVEDLIERTIAPCKTALKDAGVSPSDI
                     ADVILVGGQSRMPKVQEKVKEIFGKEPRKDVNPDEAVAVGAAIQGGVLKGDVKDVLLL
                     DVTPLSLGIETLGSVMTKLIKKNTTIPTKASQVFSTAEDNQNAVTIHVLQGEREMAAG
                     NKSLGQFNLSDIPPSPRGMPQIEVTFDIDANGILHVSAKDKATGKENKITIKANSGLS
                     EEEIQRMEEDAAKYADEDKKLRELVDARNQADSVLHSVKKSLAEHGDKIEADEKAKIE
                     DAIKELEAVAKDGDDKEVIEAKTNALMEASQKLGEKVYAEQQAQANTEGGAQPQEEKT
                     VEGDVVDAEFEEVKKD"
BASE COUNT         3669 a         3721 c         4280 g         3620 t
ORIGIN      
        1 gataccaata gagagactgc tatgaaaaca tttttgaata ccttgggtgt gatggtgata
       61 ggtgccggat tgactggatg taccagcaat ccatttgcgc cgcccaattc atcaattaat
      121 cctaaaattc agaacgaatt ggcgacatcc gcggctaagg cgcaagcaca agtcacacca
      181 ccgcctaaag aggtcactga tgaactgttt gagccactca agattgagcc acccaaagcc
      241 ttggccaaac gcgtagagcc caggttcgac ctggtcgtca acaaggcacc ggcagcacag
      301 gtgctgatgg ggattgtgtc aggcagtcct tacagtattg ccctgagccc ggagcttaag
      361 ggtgaaatct ctatcaacct gcgtgatgtg actttgtttg aagccctcaa tgccttacgt
      421 gacttgtatg gctatgagta caaaatgaac ggcaagcaaa tttttgtgga accacaaacg
      481 ctgcagacac gtgtcttcca ggtaaattac attatgggcg cccgtagagg gacctccgca
      541 acccgtgtga catcggggac cgcgagtgga tcgggtgcag gtggtgcagc tggtgtggca
      601 ggacaggccg cagcgatgaa tatctccggt agcggctccg ggacaggtac gggcaccgca
      661 actgggaatg gtacctcggg cgcttctaac ctccaggtct atgcctcgga tgtcagcatg
      721 cgcactaaca atgatttctg ggatgagatt gcgggttcac tgagtggcct gattggcgaa
      781 gaaaatggtc gcaaagtgat tatcaatgcg cagtctggcc tgatcatggt gaaggcgatg
      841 cctaaggaaa tccgccaggt agagaaattc ttgagcctga tgcaagtcac tgtagatcgc
      901 caggtgattt tggaagccaa gattattaaa gtacagttga atagaaattc ccagcagggc
      961 attaactggg cctttttaag aaaaggtgcg cgcgatgtga cgtttggtaa tgtcaacgga
     1021 aatacttccc ttggcacaac gggtaccatt accgatggga ccgtttcaag tacggcacca
     1081 ggtttccttt ccaatagctc tcccattcca ctgggtggat ctgcatttgc gcttgccgta
     1141 caaggcggta atttctcggc cttgctgact ttcctggaaa cacaaggcaa tgtcgaagtg
     1201 ctttccagtc cgcgtattgc gaccattaac aaccagaaag ccgtactcaa agtgggtaac
     1261 gatgcgttct atgtcaccaa tgtgaagacc aatgtgacaa caaccacagg cggcgcgatt
     1321 gtgacgccgg atatccagtt gcaaccctat ttctctggta tctctcttga tgtgacgcca
     1381 cagatcgaca aggatgacaa tatcatcctg catgtgcatc cgtttgtcag tgatgtgacg
     1441 caggtcaacc gtaccgttac cttgtccagt gcgaatggtg gtacttacac cattccaacg
     1501 gcctcctcca atattaacga aacggatagt atcgtgcgta ccagggatgg cagcgtgatt
     1561 gcgattggtg gcctgatgtc tgagtcagtc gacaatacac gtaccaaagt ccccgggttg
     1621 ggggatgtga agttcttggg taacctgttc cgccagaaag acctgagcag cactaaaaca
     1681 gagttggtga ttttgctcaa gtctactgtt gttcgtagtg gtgagaactg ggcgcaagat
     1741 atgcttgaaa gtcaggaccg ggttgataaa ctgaaagctg atacagcttt ttaggagcgg
     1801 gcatgtacct gcaacatttt ggcctgaagg aatatccatt taccatcacg ccggatacca
     1861 gtttttatta tgcgacgcgt agcctgcaag aagccctcaa tacgctgctg attgccatcc
     1921 agtctggcga gggctttgtg aagattaccg gtgaagttgg caccggtaaa accctgttat
     1981 gccgtcgttt acttaaagca ttggcctcca attgcaagat tgcatttatt cccaatcctt
     2041 accttgatcc gcatgcttta ctcatgacgc tcgccaccga gtttggcgtc gcattcaagc
     2101 ctgactttac ccatcatgac atgatcgatg cgctcaaccg caaactgctc acctttgctg
     2161 aagaggggca acatgtggtg gtgtgcctgg atgaagtgca agccatgcca ctggagacgc
     2221 tggaggcttt gcggttgctg agtaatttgg aaaccgaaaa gcgaaaactg atacaagtgg
     2281 tgatttttgg ccagccagaa cttgaggagc gcattaacca tccgtctatt cgccagttac
     2341 gtcagcgtat cggtttcgag taccatctca aaggcttgca atggtcggag ttagggtttt
     2401 acctgaatca ccggatgcat gttgcaggct accagggcgg catggttttt actcctgcca
     2461 gtatgcgatt gttgtatcgt tatgccaacg gcatcccgcg gctactgaat attctggcgc
     2521 ataagtcgct gctttctgca tttggacgag gccagcaagg tgtctctgcg gaagatgtga
     2581 aagccgccat tctggatacg caagccagtg tcaaagcaca acaccgttgg cttggacatg
     2641 ggtttaaatg gtggctggca ttccacacgg ctatggccgg ctggggccgt tagttgaagt
     2701 attggggaag ccgttgagtt taattaacca ggtattgcaa aatgtcgagg cccgtcaggg
     2761 tggtgtagcc atggatggca agccagatgc tgctggctgg agtgctcagg tgaagcctgt
     2821 gttgtcgcat caaggtgggc attccggtcg ctggctccgg cgcctggcat ggggattggt
     2881 gttgctgaca ttggcagggt ggttgctgat gaatcggcca atgctgatgg cttattttca
     2941 accttcacca ttcgctgcca cgcagaacaa gggtattgcg acacaagtcg aggcgccatc
     3001 cgcggcagct aaaccagagg ttgaggctgt ggctattcca gcaccagcaa tttcgagtac
     3061 cccgcaatta accaggtctt tattcagtgc ttggcagtcc gagtctcctg cggcagtcag
     3121 tcctgtagcg cctcgtcatc agccagtcgc taatgctgcg cctgctaaag cagtcccagc
     3181 gccacaagta gaaggcgaat tcagtatcaa gccggctgaa gcgactgccg ccaatccggt
     3241 ggtttcggtg atcgagccac cgttagaagc agcaaatacc gcgaaacagg tcaaaagcaa
     3301 tcaagaagag gctgtggtta gaggcgtggt caacaagcag gtccgtcccg atcaggaagt
     3361 caatttgctg attcagcgcg ctgtggatca cgaacaaaaa ggacgcctta acgaggcatt
     3421 ggtcacattg cgacaggcca taaatagcta tccgcaatca gaggatgcac ggcagttgct
     3481 ggcagcttat ttgttcgagt caaaacaaga ttctgaggcg gttgctgtgt tgcaaactgg
     3541 catcaagcaa tatcccgggc agattggttt atccaaatca ctggctaaat ggcagctttc
     3601 acatggccag ccagagcctg tgctgcagac cctgaacccc gtcgccaatc tgctgatcca
     3661 ggatgcggag tcacaatgga tgcttgccat ggcttaccag caaaccgggc aacatgcagc
     3721 ggctttgccg cactttgagc gtgcgatggt gttgcggcca ggcgtggcgc agtggatggt
     3781 ggcttacgcg atttcgctgc aggctgcggg ccagtctgct caggcattgc agcaattaca
     3841 gctggcttat aacatgccac tcagcgagcg gttatctgag tttgtgagtc aacgtattcg
     3901 ccaattgggc ggcaccgtcc cggcgcgcaa cgaataggca cactcaagca aagccccaca
     3961 gcgcgggcta cccgcggtga attatgtcct agcttttcaa ggtgcgtgcc aacaccagtg
     4021 ccgttacccg cttggcgcct gcgtttttca atactttggc gactgcgtgc atactggcac
     4081 ccgttgtcat gacatcatcc actaccacga catgtttgcc atgccattgt gattgcgtgg
     4141 taaacgcacc acgcaagctg cgtgttctgg ttttcatatc catgcctgct tgtgttggcg
     4201 tatcaatgac tcttgtacaa ccatgtattt cgagtggaat ctgccagtgt ctatgcacct
     4261 gctttgccag ttccagggcg tgattgaagc cacgctccct gacgcggttc ggatgcatgg
     4321 gcatggccat gagaatatgt ggttgttccg ggccagggaa agttggcaag cggtcgagca
     4381 ggctagttgc cagcaggtgg ccaagggaaa gttgttgatg gtatttaaac tggtgcaata
     4441 gacggtccag cggatacgcg tagcggaatg ccgtcaatgt atgatcaaag gcgggggcat
     4501 gttgcagaca gatcccacat aatgtgcccg agacattagg aagtgcacat tgtggacagc
     4561 gaggcgcggt ataccaaggt aataattcga cgcaatgggc gcaaaggatt gaatggcgtt
     4621 tgttagctgt ggctggcgca gcacagagtg tgcatggcaa tctggattcc agccagttct
     4681 gtaatcgaag gtacgacagg atttgattaa attttaatct gttgtaaaaa tttttcacgt
     4741 gggattttaa acaacatata attcggccag atttttcatc tatttttctt gcgtggtgct
     4801 tatggaacaa caaacttctg ctgccggttg tggcagcgaa atgcaaacct cagtcattaa
     4861 cctggacggc ctgaaaccat cggctagttg ccatcctaca gttgaacgct ggcgcgtagc
     4921 agacatcatg gcattgtttg aattgccatt caatgatttg ttacatcagg cacacactgt
     4981 ccatcgtgag cattttgatc ctaacgcggt gcaagtgagc actttactgt caattaaaac
     5041 aggcggctgt tcagaggatt gcggctactg cccacaagcg gcgcgttacc acactgaagt
     5101 ggaaaacgag ccattgatgc agttggatga agtgcttgcg gctgcccagg cggctaaaga
     5161 cagtggtgcc agtcgcttct gcatgggcgc tgcctggcgc ggccccaagc agcgtgacct
     5221 ggaacctgtg ctcaaaatga tttctgaagt gaaggccatg ggcctgcaaa cctgtgccac
     5281 cctcggcatg ttgaaagacg gccaggctga gcagttgaaa gaagccgggc tggattatta
     5341 caaccataac ctggacacgg caccagaatt ttacggtgat gtgattacga ctcgtactta
     5401 tcaggaccgt ctggataccc tggaccgtgt acgcagtgtg gacattaatg tctgtagcgg
     5461 tggcattatt ggcatggggg aaacccgtac ccaacgtgca ggcctgattg cgcaactggc
     5521 caacatggaa caaccaccag agagcgtacc gattaacctg ctgacgcaag ttgagggtac
     5581 gcccttgcat ggtaccgatc cactggaccc cctggagttt gtacgtacca tcgcgatagc
     5641 ccgtatcact atgccgacta gttttgtgag attgtctgca ggccgccagg gtatgggcga
     5701 agcggtgcaa gccttgtgtt tccatgcggg cgccaacagt attttttatg gcgaaaaact
     5761 gctgaccacg ggtaatccag aggttgaagc ggataatgct ttgtttagca aactgggcct
     5821 gaagaagatt taatgagcga tttgctggaa tccttacagg ccgacctgga aaaacggcaa
     5881 acacagggcc ttatgcgtca gcgacgcctg ctcgactcac cgcaggcaga acacatcacg
     5941 gccaatggcc aggcttttct gtcgttttgc agtaatgatt acctaggctt ggcgaatgat
     6001 gctgcgctgg tgagcgcctt gcagcaagca gccgcagaag cgggtgttgg cagtggcgcc
     6061 tccaatttaa tcaccggaca tcatcgctac catgatcaac tggagcaggc gttggccgcc
     6121 tttgtgggca agcctgctgc gttgttattt tctaccggct atatggcaaa cattggtgtg
     6181 gtgagtgccc ttatggggcg ggatgacgcc gtgttttcag acaagctcaa ccatgcttgc
     6241 ctcaatgacg gggcatttct gtcgcgtgcc acgcatcacc gttttccaca caatgatgtg
     6301 gcagccctgg aaaaattgct gcaagccagc acggcgcgac ataaactgat tacggcagat
     6361 gcagtattta gcatggacgg tgatcttgcg ccgctggccg cttacctgga actctgtgag
     6421 cgctacgatg cctatttgta tgtcgatgat gcacatggct ttggggtgtt gggcccgcaa
     6481 ggccgtggat cactcaggca tctgggcttg caatcaccgc gcctgatcat gatgggcacg
     6541 ctgggtaagg ccgccggtgt agctggcgcc tttgtcgctg gtgaaaaaat cgtgatcgac
     6601 tacctgatcc agtatgccaa cagttatatc tacaccacgc cagcgccacc tcctttgtca
     6661 gctgccttgc tggcctcggt taaggagatt gagcacggcg aggcacgtag aaaacagctg
     6721 tttgacatga ttgccttgct taaaagtgag ctgtcactca cgcgctggca tctgttgcct
     6781 tctgaaacag cgattcagcc gctgatcgtc ggggataccc acgaggcgct ggcattgagc
     6841 cagtatctgc aagtgcgcgg tattttggtg ccggctatcc gcccacctac tgtaccggcg
     6901 aatacttcac ggttgcgtat ttctctgtcg gctgcgcata gggaggccga tgtgcgcgca
     6961 ctgattgcgg cattgcatgc cgctgaacgc gagcttccag tcgggataca ggcatgagtt
     7021 gctttattga aatcatgggg cagggtaagc cattagtctt gttgcacggt tggggcatga
     7081 atagcgccgt ctgggagcgc atcaccaagc gcctgtctgc caactaccga ctgttcctgg
     7141 tggatttgcc gggtatgggc cagagtcggc cggtataccc ttatcatctg catagcctgg
     7201 ccgaggcggt ggcggaggag atccctggtg tatccagcat cttgggctgg tctctcggag
     7261 gcctagtcgc gcaacaaatt gcactcaatc agccggaccg tatagaaaag ctgatcctgg
     7321 tcgggactaa tccctgtttt gtcagcaaac ctgactggtc gcatggaata gaagcgcgtc
     7381 atttcgagca gttcaatgag cggttcgcca aagactttaa tgccaccttg ctcaacttcc
     7441 tgaccttgca gtgcatgcat gccaaagatg cccgcagcac cgtgcgccaa ttacgcattg
     7501 cctttggcgc caaacctacg ccaacgcaag atagcctgaa ccaggcgctg gatatcctgc
     7561 tacagacaga cttgcgcgaa gaagtgggac gcttgttcca gccgacactg atcatacacg
     7621 gcgaccgtga taccctggcg ccggtctcgg ccgcacactg gctggccatg catttgcaga
     7681 aggcgcgctt gcgagtgatc gctggtgctg cacatgcccc tttcctgtcg catgccgatg
     7741 ctttctgtga gactgtcgaa gcctttatgg ctgatgagtc ggtggagaac taaatgcagg
     7801 atgtctatca gatcgacaag tcgcgcatgc ggcagtcgtt tcatcgtgcc gccaggcaat
     7861 atgacgcggc cgccattctg caacggcagg tgcgcgagga aatgttgagc cgtcttgagg
     7921 tggttaagct ggagccgacc gtgattctgg atgctggctg tggcaccgga catgggctta
     7981 gtgccttgct taagcggttt aagaaagcgc agggcattgc ccttgatatt gccgaaggca
     8041 tgttgtcgcg cagtaaaaca ttattcccct ggtaccgttt ctggcaaact tcaccccgtt
     8101 ttatctgtgc agatattgag gcgatgccga ttgcaactgg tagcgtggat ttggtctggt
     8161 ctaatctggc tgtgcagtgg tgtaatgaca tggataccgt gttgcaggag ttccggcgtg
     8221 tgctcagacc agatggattg ctgatgtttg caacgctggg accagataca ctgaaggagt
     8281 tacgtgcggc ttctggtagc gagcataccc atgtcagccg ttttattgac atgcatgaca
     8341 ttggcgatgc actgacgcgt gccggcttta gcgcgccagt gctggatgtg atgcattaca
     8401 ccctgaccta cgacacggtt gagtctgtga tgcgtgacct caaagccatc ggggcgcaca
     8461 atgctaccgc tgggcgtgcc aaaggcttaa gcggtaaagg ttttttgcaa aaactgcgcc
     8521 agggctatga cgttttcagg cgcaacggta agttgccagc cacttatgaa gtggtgtttg
     8581 tgcatgcctg gacgggcagc cagccttata ctgcgcctgg caccagtcct gtacagttta
     8641 tgccgcgtaa agacgcacaa aggtaagtca tgcacgcgtt accacgtcat ttttttgtga
     8701 ccgggaccga tactggcgtg gggaaaacca cggtgactgt acgcctgatg cagcagctta
     8761 ttgcgcaggg tttcactgtg attggcatga agccggttgc ttcgggctgt gagtggctgg
     8821 ctggccgctg gcaaaatgaa gatgtccgcc agttaacggc ggcctcgaat gtgaaggcgc
     8881 cgcctgagct gattaatccg tattgttttg atccacccat tgcgccgcac attgccgctg
     8941 aacaagcagg cgtcagcatt gacctgagtg tgattcaatc tgcctatacg cagctgacaa
     9001 ccatggcgga tgtggtgatt gtcgaaggtg ccggtggttt gctggtccca ctcaacagca
     9061 agcaatcact ggcggattta attcagctct tgaatattcc tgcgttagtg gtggttggca
     9121 tgcgtttggg ttgcatcaat catgcactgt tgacggcaca agtattgaaa caacgaggga
     9181 tccgtttttg tggctgggtg gcgaacagca ttgaccctca gatgtctatg ccagaagaaa
     9241 acttgcagac cttgatagaa agtttgcagc aagcgccctt attagaaatc cctttccagg
     9301 aatcgtcgac ctagatggaa tcctcattac tgacccgcat agaattgcgt gcctcggtgc
     9361 cacccgtaac caaaggcttg atcgcgttga atgtgctggt gtttgtggtg atgctgtttg
     9421 gcggggccgg gttctggcat tcgccgaata ccgtacaact gacctggggc gctaactttg
     9481 ctccggccac ggcagatgga caatggtggc ggctgggatc tgccatgttt ctgcattttg
     9541 gagccttgca cctgggcatg aatatgctgg ccttatggga cggcggcaaa ctggtcgagc
     9601 gcatgtttgg cgcagtacgt ttcctggtga tttacctggt cagtggcgtg ggtggcaatt
     9661 tgttgtcctt ggtcatccag ggtaatgatg ccgtctctgg cggtgcctct ggcgcgattt
     9721 ttggcattta tggtgccctg ctggtttatg tttggttcgg caggcgccag atgcaggtgc
     9781 aggaattccg ctggctgttt tggggggcgc tgctgttttc tacgcttacc atcgtcatgg
     9841 gatacgtgat tcccggcatt gataattcgg cgcatattgg cggatttgtc accgggatgg
     9901 tgatggcggc gcttttcttg ccatctagca tcttgccgag caggccatta atgatcacgc
     9961 aaagtttggc cgcggtggcc tggatggcag cggtagttgt gttgctgacc catttgccta
    10021 cgcccaagta tcgctggcag caggaagtgg cgatacaaaa acaagtgcaa aaactcaatc
    10081 aggttgatca gcgggcacag caaaaatggc ggaagattat gctcgaaggc cgcgagggca
    10141 gtgtcagttt tgatgaaatg gccacacaga ttgatactga agttgccacg ccttatgaag
    10201 aaagctttga aaaactatct gcgctgccag aagatcccaa gctaccttca gcagctttgc
    10261 ttgagcaagc caaggcctat gcattaaagc gtagtgaggt cagtaaggcc gcggcagaca
    10321 agttacgcaa tatgccgttt acgccaggtc gtcccgctag cgagtaatcg cgctattttt
    10381 cggtagctta agcgttgact aactcaatga tcctggcttc aggagctgca atttgcgagc
    10441 gcgccatctc ttgtacaaag gccttggcat gttcgctcca ggattgtgtt ggcaactgct
    10501 ctatcatgcc tcgcagaacc aggttcatct gcgcttggtt aaatggctca ggcagcactt
    10561 caccggcttg cgccgttgac acatagcctg cataaccaca aacctcagtc accagtaacg
    10621 gcaggccggc agtcatggct tcaattaaga cgatgcctgc caattcagag cgtgccgcat
    10681 gcaccagtaa atcgcaaccc agcatcatgg cgggaatgtc tgggcgccca cctgcttcga
    10741 tacagcgatc cgcaaccccc agtttttccg cgtaagcttt catattgctg ctggattcaa
    10801 attcgcccac cgctagcaac cagcagcgct gcttcagtgg cgtgggtagg ctgcttaatg
    10861 cgtctatcgc ccggtctacg cctttacgca caaacgctga gcccacggtg agcaacacga
    10921 tggcgtcctc tggcagcttg aaagcctgcc gcacataggc tcggtagtct tgcgtcggta
    10981 gggcgatgaa gcgttgcaac ggaatgtttg gggggatcag gtggaagcgt ttactctcag
    11041 taccatacca gcgctggaat atagcctggt cgtgctggtt gagcagcaat atatgcgtcg
    11101 tagcctgttt gccaaacacg gcgttttcca tggtggaaaa aaaacggtag cggccagaaa
    11161 gttttttata ccagggttca cgggccatgc gctcggcgta gcaggggtca gccgcaaaat
    11221 agacatccag cccaggcatg cggttaaagc cgaccacgcg gtcaaaatcg ccttgagtaa
    11281 tcgcttgccg catttggcgg atcagtgact ggtgtctgtg gtgattgagc cagccagtgg
    11341 ccttgagcag gttgactgtg atgtcttgcg ctacatcgcc gcgccattcg ccagtaaaga
    11401 ttgtgacttc atgcccctgg gcgacgcagt ccctggcaat gcgcaacatg tcgcgctgca
    11461 cgccgccata gggaaaatat ttgaatatga gaaaagccaa cttcatgcgg tggcattgta
    11521 tgcgaaaata gcggccatga aacgcattct gattgtcaaa acgacctcca tgggcgatgt
    11581 gatccatgcc ctgcctgtgg ttgaagatat tctccagcat tttccggacg cccatatcga
    11641 ctggctggta gaggaaagtt ttgccgatat tccgcgcctg caccctcgtg tgcaacaggt
    11701 gtttaccgtc gccgtgcgcc gctggcgtaa acaatggtgg cattgccaga cctggcgtga
    11761 aatcaccact gtgaaacggc aggtgggtac ccaagcttat gatgtggtgc ttgatttaca
    11821 aggcctggtc aaaagtgcgg tcatggcttg ctgtgcacat ggcccacgcc atggctatga
    11881 caaacattcc attcgtgaac cactggccag ccgtttttac ccgcatcaat atgccattac
    11941 ctaccgccag catgcggtga tccgcatgcg cacgctggca gccctggctt taggctatgc
    12001 cgtcccgcaa gacaaaccgg cctatggtct tgttactgat gagcatcatt ctaaacagcc
    12061 tgctttcctg gcattgcatg ccaccagtcg tgacagcaag ttatggccgg aggatcactg
    12121 ggttgcgttg gggcaatatt atgcagccca aggactgcac atgtggttgc cctgggcaag
    12181 tgccaatgaa caggcgcgcg cggaaagaat tgctgctcaa gtgccgcaag cattggtctt
    12241 gcctaaactg agtttgcagc agctggctgc acaaatgcct caggcgcggt ttgccgtagg
    12301 cgtggatacc ggactttcgc atctggctgc cgccctggat attccggttg ttgcgatcta
    12361 taccgatacc gagcccgcgt ttactggggt cgccgagggg cgaagggcac ctgcagttaa
    12421 ccttggcgga aaaggaaacg tccccaccgt tgaagcggtg attgcacagc tgagcgtaat
    12481 ctcggtggcg gttgaccaga tttaaatcat atattcttca cctttaccct tgaaatcagt
    12541 aggggcatcc ccaacattct gtaaatattg agagaaaaag tgttgttagc cttctctctg
    12601 gtagaacagt gttgaacaag aaggggaccc ccatgcaaga accaaataca cctgccgacg
    12661 taaatgcaaa tgaagcagca gtgaatgaag ccgcaccaga catccaagtg gaagacactc
    12721 agctagcacg cattgccgaa ctggaagctg cgctcgaaga agccaaggca cagaccttat
    12781 atgtgaaggc ggaaggtgaa aatatccgcc gtcgtgcgat ggatgatatt gataaggccc
    12841 gcaaatttgc gctggagaaa tttagtggtg agttgctggc agtgaaagat agcctggatg
    12901 cggcattggc cgtagagagt gcagatgtgg ccagctacaa gaacggtgtc gagttaacgg
    12961 ctaaacaatt gctcagcgtg tttgaaaaat tcaatattgc tgaaattaat ccggtcggtg
    13021 agaaatttga tcctaacaag caccaggcga tcagcatggt gcctgccgaa ggcgaaccaa
    13081 ataccgtctt gagcgtattg caaaaaggct atgccttaaa tgatcgcgta ctgcgcccgg
    13141 cattagtgac agtcattcag gcaaagtaaa tcaaccacat ttgaagtggg gacttgaaat
    13201 ctattcaggc atcccaatta ttacatcatt gatgttgtga ttaacgaatt ttaaggaaaa
    13261 cataaagatg gcaaagatta tcggtattga tttgggcacc actaactcgt gcgtggctgt
    13321 gatggaaggc ggaaagccac gcgtgattga aaacgctgaa ggtgcgcgta cgacgccttc
    13381 tattatcgcg tatcaggaag acggcgaaat tttggtgggt gcacctgcaa aacgtcaggc
    13441 tgtgaccaac ccgaaaaaca ccctgtttgc ggtgaagcgt ttgatcggcc gccggtttga
    13501 cgagaaagaa gtgcaaaaag acatcgactt gatgccttac accatcacca aggctgataa
    13561 cggtgatgca tgggtagaag tgcgtgacaa aaaacaggcg cctccacaga tttctgctga
    13621 agtgttgcgc aagatgaaga aaactgccga agactacctg ggcgaagaag tgaccgaggc
    13681 cgtgattacc gtgccagctt actttaacga cagtcagcgt caggcgacta aagatgctgg
    13741 ccgtattgct ggtctggaag tgaaacgtat catcaacgaa ccaaccgctg cggcgctggc
    13801 atttggtttg gacaaacagg aaggtgaccg caagattgcg gtgtatgact tgggtggtgg
    13861 taccttcgat atttccatta tcgaaattac tgaaatcgac ggcgaacacc agtttgaagt
    13921 gttatctacc aacggtgaca ccttcttggg cggtgaagac ttcgacaacc gcatcatcga
    13981 ctttttggca gacgagttca aaaaagaaaa tggtttggac ttgcgcaatg acttgctggc
    14041 aaaacagcgt ctgaaggaag ctgctgaaaa agccaagatt gagttgtccg gtgcacaaca
    14101 gaccgaagtg aacctgcctt acattacggc agatgcgacc ggtcctaagc acttggtggt
    14161 gaaaattacc cgtgccaaac tggaatcact ggttgaagac ctgattgagc gtacgatcgc
    14221 gccttgtaag actgccttga aagatgcagg cgtgtctcct tctgacatcg ccgacgtgat
    14281 tttggtcggt ggtcaaagcc gtatgccaaa agtgcaggaa aaagtgaaag agatttttgg
    14341 caaagagcca cgtaaagacg tgaacccgga cgaagccgta gcggtaggtg ctgcgattca
    14401 gggtggcgtg ttgaaaggtg acgtcaaaga cgtattgctg ctggacgtga caccgttgtc
    14461 tctaggtatc gagactttgg gtagcgtgat gactaaactg atcaagaaaa acaccacgat
    14521 tcctaccaag gcatcacaag tgttttcaac cgcggaagac aaccagaatg cagtgacgat
    14581 tcacgtgttg caaggtgaac gtgaaatggc agctggtaac aagagcctgg gtcagtttaa
    14641 tctgagcgat attccaccat caccacgtgg catgccgcaa attgaagtga cgtttgatat
    14701 cgatgccaac ggtattttgc atgtgtctgc caaagacaag gcgactggca aagagaacaa
    14761 aatcacgatc aaggcgaact caggcttgtc tgaagaagaa attcagcgca tggaagaaga
    14821 tgccgctaaa tatgcggacg aagacaagaa gttacgtgaa ctggtcgatg cccgtaacca
    14881 ggctgacagc gtattgcaca gcgtgaagaa atcactggct gaacacggtg acaagattga
    14941 agccgatgag aaagccaaga ttgaagacgc tattaaagag ctggaagctg tggccaagga
    15001 tggcgacgac aaagaagtga ttgaagccaa aaccaatgca ttgatggaag cttcacagaa
    15061 actgggcgaa aaagtctacg ctgagcaaca ggcgcaggcc aataccgaag gcggcgcaca
    15121 accacaagaa gaaaaaactg tggaaggcga cgtggtagat gccgagtttg aagaagtgaa
    15181 aaaagactaa cttaaatggg caagtggcga cacttgcccc tctttatgat gcaggcaaac
    15241 acacctcgct gataacgggt gtgtttgttt acatggcaca aacgcatgat
//
LOCUS       DATMSK010000134         4528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_104073,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000134 DATMSK010000000
VERSION     DATMSK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104073"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /gene="typA"
                     /locus_tag="VNZ84_04625"
     CDS             complement(<1..1771)
                     /gene="typA"
                     /locus_tag="VNZ84_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWU66272.1"
                     /translation="MARNLRNIAIIAHVDHGKTTMVDKLLQQSGTFASHQAVTERVMD
                     SNDLEKERGITILAKNTALTYEGTHINIVDTPGHADFGGEVERVLGMVDGVLLLVDAV
                     DGPMPQTRFVTKKALALGLKPIVVINKVDRPSARPDWVINHTFDLFANLGATDEQLDF
                     PVVYASALNGFATLDMSKPSDNMRALFDTILSHVEPPKGDSDAPLQLQISALDYSTYT
                     GRLGVGRITNGRIKPGQVVAVMREDEQVAQGRINQVLGFKGLERVPVEEAEAGDIVII
                     SGIEEIGIGFTIADREKPVGLPHLGVDEPTLTMDFMVNTSPLAGTEGKFITSRQIRDR
                     LTKELLVNVALRVEDTEDTDVFRVSGRGELHLTILLENMRREGFEIAVGKPRVVYREI
                     NGEKCEPYEILTVDVEDENQGAVMEELGRRRGEMTNMESDGNGRTRVEYRIPARGLIG
                     FQGEFLTLTRGTGLMAHIFDEYAPVKADMPGRRNGVLISAEQGEAVAYALWKLQDRGK
                     MFSSPGDRLYEGMIIGIHSRDNDLVVNPIKGKQLTNVRASGTDEAVRLIPPVSLSLES
                     AVEFIDDDELVEIIPKTIRLRKRY"
     gene            2156..3718
                     /locus_tag="VNZ84_04630"
     CDS             2156..3718
                     /locus_tag="VNZ84_04630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU66273.1"
                     /translation="MSFADLNLDPSILRALTDAGYENPTPIQAQSIPEALQGRDIMAS
                     AQTGTGKTAAFTLPALNLLATQHPVRSRGPRILVLTPTRELAAQVNDAARKYGKFLRA
                     RCISIVGGMPYPLQNKLLSQPLDILVATPGRFIDHLERGRIDLSRLQMLVLDEADRML
                     DMGFMPDVERICEALPADRQTLLFSATLDGIMDIGKRFLRDPVTIQVAGQKERHANIE
                     QRLHFVDDMNHKNKLLEHLLIAPDVNQTIIFTSTKRHADLLAEDLYAAGHKTAALHGD
                     MTQGARNRTLTKLRHGDVKVLVATDVAARGIDVQGISHVINYDLPKFAEDYVHRIGRT
                     GRANNTGVAISFASNADRHLLRKIEQYTGQKLQAEVIAGFEPKRAARMDDGEGRRGGK
                     RNGHGKPQGKGGFRRDDRQAKSFGERVAREGNREGSNRETQGNKPRAPRQTEQRQDKE
                     VNGNSKHYNKDTDFQFARALADEFGQRRPRPAGNNQERFGNPNANRGNNKPRPAGKSF
                     GDSGVKRPRSFV"
     gene            3728..>4528
                     /locus_tag="VNZ84_04635"
     CDS             3728..>4528
                     /locus_tag="VNZ84_04635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU66274.1"
                     /translation="MSEQTTVDFRALGLAEPLLKAIEESGYTTPTPIQAQAIPLALQR
                     MDLMAGAQTGTGKTAAFSLPILHTLLPLASTSTSPAKHPIRALVLAPTRELAIQVYDN
                     IKTYAKHTALRSLVVYGGVDIKTQTPHLKNGVEILVATPGRLLDHVEQKTLSLSQVQF
                     LVLDEADRMLDMGFMPDLKRILALLPKQRQNLMFSATFSDEIKKLADAFLNNPTLIEV
                     ARSNATNDNVEQKAYLVNNDQKQRFLVQLLKQYTNQQVIVFTKTKLSAS"
BASE COUNT         1184 a         1235 c         1023 g         1086 t
ORIGIN      
        1 gatagcgctt acgcagacgg atggtttttg gtatgatctc aaccaactca tcgtcatcaa
       61 taaactctac tgcagattcc aggcttaatg agactggcgg aatcaaacgc acagcttcat
      121 cagtaccgga agcacgcaca ttggtcagtt gtttaccttt aatcgggtta actaccaagt
      181 cattatcacg actatgaata ccaatgatca taccttcgta caatctgtcg ccagggcttg
      241 agaacatctt gccgcgatct tgcagcttcc acaaggcgta agctaccgct tcaccttgtt
      301 cagctgaaat cagcacacca ttacgacggc caggcatgtc tgctttcaca ggtgcatact
      361 catcaaaaat atgtgccatc aagccagtac cacgggtcaa tgtcaagaat tcaccctgga
      421 aaccaatcag gccacgcgct ggaatgcggt attcaacacg ggtacggcca ttgccgtcgg
      481 attccatgtt agtcatttca ccacgacgac ggcccagttc ctccatcacg gcaccctggt
      541 tttcatcttc aacgtctact gtcagaattt cgtatggctc acatttttcg ccattgattt
      601 ctctgtaaac tacgcgtggt ttacccacag cgatttcaaa accttcacgg cgcatgtttt
      661 ccagcaatat ggtcaggtgc aactcaccac ggccagatac gcggaatacg tcggtatctt
      721 cagtgtcttc cacgcgcagt gccacgttca ccaacagctc tttagtcaaa cggtcacgga
      781 tctgacgtga agtaataaac ttaccttctg tacctgccaa tggagaagtg ttcaccataa
      841 agtccatggt cagggttggc tcatccacgc ctaaatgtgg caaaccaact ggtttttcgc
      901 gatcagcaat cgtgaaacca ataccgattt cttcaatacc ggaaataatc acgatgtcac
      961 ccgcttcagc ctcttcaact ggcacgcgct ccaagccttt aaagcccagc acctggttga
     1021 tacggccttg tgcgacttgt tcatcttcgc gcatcacggc aaccacctgg cctggcttta
     1081 tacggccatt agtaatacgg ccaacgccca agcgaccagt gtaagtcgag taatccaatg
     1141 cagaaatttg caattgcaac ggtgcatcgc tgtcaccttt aggtggctcg acatgactta
     1201 aaatcgtgtc aaacaacgcg cgcatattgt cagaaggctt gctcatgtcc aatgtagcaa
     1261 agccattcaa tgcggatgcg tataccaccg ggaagtccaa ttgctcatca gtcgcgccca
     1321 agttagcaaa caggtcaaaa gtatggttaa tcacccagtc aggacgtgca cttgggcggt
     1381 ccactttgtt aatcaccacg attggcttca aacccaaggc caacgctttt ttagtcacga
     1441 agcgggtttg tggcattgga ccatccaccg catctaccaa cagcaatacg ccgtccacca
     1501 tacccaacac acgctctacc tcaccaccaa agtcggcgtg gcccggtgtg tcgacgatat
     1561 taatgtgtgt gccttcatag gttaaggccg tgttttttgc cagaatggta atgccacgtt
     1621 ctttttccag atcgttgcta tccatcacac gctcagtcac tgcttggtgt gaagcaaaag
     1681 taccagactg ttgaagtaac ttatcaacca tggtggtttt gccgtggtca acgtgggcga
     1741 taattgcgat atttctgaga ttgcgtgcca tgtgaactgc catatgtttg gaaagggcgc
     1801 gattttatca caaatcaact aaataattga cttttaaaat aatttggcta taatgcgagc
     1861 aaccttaaaa cgtagcaggt acttgacccg ctaatttacg ttttaaattc attgccctga
     1921 cctgtcgata actcgcgaaa ttttgaggtt atcatcttta gatgaagtct tcatgacaca
     1981 tcttctttta tcggttagcg gccatgccca agcgccggta aaagcaattc gttcccattg
     2041 atgttgcttt ggcttagttg atattcaccg taccttagat ttgtgacggt catcacatga
     2101 gctctgcttt tgctttacta gcgccctttt taattgttga tgaaagggac attccttgtc
     2161 ttttgcagat ttaaatcttg acccctccat tttgcgcgca ttgaccgacg caggctatga
     2221 aaacccaaca ccaattcagg cacaatccat tcctgaagca ttgcaaggcc gtgacatcat
     2281 ggcctcagca caaaccggca ccggtaaaac ggctgccttt accttgccag ccttaaacct
     2341 gctggcaaca cagcatcctg ttcgcagccg tggcccgcgt attctggtgc taacaccaac
     2401 acgcgagctg gcagctcagg tcaatgatgc tgcacgtaaa tacggtaaat tcctgcgcgc
     2461 acgttgcatc agcatcgtgg gtggcatgcc ttacccatta caaaacaaat tgctgtcaca
     2521 gccactggat attctggttg caacgccagg ccgttttatt gaccacctgg agcgcggccg
     2581 tattgacttg agccgtctgc aaatgctggt acttgatgaa gctgaccgca tgttggacat
     2641 gggctttatg ccagacgttg agcgcatttg cgaggccttg cctgccgacc gccaaaccct
     2701 gctgttctct gcaacactgg atggcatcat ggatatcggc aaacgcttct tgcgcgaccc
     2761 agtgacgatt caagttgcgg gccaaaaaga aagacatgcc aatattgagc agcgcctaca
     2821 ctttgtggat gacatgaacc acaaaaacaa actgctggag cacttgctga ttgccccaga
     2881 cgtcaaccaa accatcatct ttaccagcac caagcgtcac gctgacctgc tggctgaaga
     2941 cttatatgct gcgggccata aaacagctgc attacatggt gatatgacac aaggcgcgcg
     3001 taaccgcacg ctgaccaagc tgcgccatgg cgacgttaaa gtgttggtgg ctaccgatgt
     3061 cgctgcacgc ggtatcgacg tacaaggcat ttcacatgtg attaactacg acctgccaaa
     3121 atttgcagaa gactatgtcc accgtattgg ccgtaccggc cgcgccaaca acactggcgt
     3181 tgcgatctca tttgcctcta acgctgatcg tcacctgctg cgcaaaattg aacagtacac
     3241 cggacagaaa ctgcaggctg aagtgattgc cggtttcgaa cccaaacgtg cagcgcgcat
     3301 ggatgacggt gaaggccgtc gcggcggcaa gcgcaacggc catggcaaac cacaaggtaa
     3361 aggcggcttc cgtcgcgatg accgtcaagc taagtctttt ggtgagcgcg tcgcgcgtga
     3421 aggtaaccgt gaaggcagca atcgtgaaac acaaggcaac aaaccacgtg caccccgcca
     3481 aaccgaacaa agacaagaca aagaagtcaa cggcaacagc aagcactaca acaaagatac
     3541 tgacttccag tttgcacgcg cattggctga cgagtttggt caacgccgcc cacgtccagc
     3601 tggcaacaac caggagcgct ttggcaaccc gaatgctaac cgtggcaaca ataaaccacg
     3661 cccagccggt aagtcatttg gcgattctgg cgtaaaacgt cctcgttcat tcgtttaagc
     3721 ctagcctatg tctgagcaaa ccacagtaga ttttcgtgcg ctcggcctgg ccgagccgct
     3781 gttaaaagcg attgaagagt ctggttatac gacacctacc ccgattcagg cgcaggccat
     3841 tcctttggct ttgcaacgca tggacttgat ggcgggcgcg caaacgggta ctggcaaaac
     3901 ggcagcgttt tccttaccta ttttgcatac gctattacca ctcgccagta ccagtacttc
     3961 cccggccaag caccctatcc gggccctggt gttggcgcca acgcgtgaac tggcgatcca
     4021 ggtgtatgac aacatcaaaa cctatgccaa gcataccgct ttacgtagcc tggtagtcta
     4081 cggtggcgtt gacatcaaaa cccagacacc acatttgaaa aatggcgtcg aaatcctggt
     4141 agcgactcct ggccgcttgc tagaccatgt tgagcaaaag accttgtcat taagtcaagt
     4201 ccagtttctg gtgctggatg aagctgaccg catgctggat atgggcttta tgccagacct
     4261 gaaacgcata ctggcattgc tacccaagca acgccagaac ctgatgtttt cagcgacctt
     4321 ctctgacgaa atcaaaaaac tggcggatgc ctttttaaac aacccgactt taattgaggt
     4381 ggcacgcagt aacgccacca acgacaatgt cgaacaaaaa gcttacctgg taaacaatga
     4441 ccagaaacag cgcttcctgg tacagttgct caagcaatat accaaccagc aagtcattgt
     4501 gtttacaaaa acaaaactct cagccagc
//
LOCUS       DATMSK010000135         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_362089,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000135 DATMSK010000000
VERSION     DATMSK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362089"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /gene="ccoG"
                     /locus_tag="VNZ84_04640"
     CDS             <1..1023
                     /gene="ccoG"
                     /locus_tag="VNZ84_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HWU66275.1"
                     /translation="LWMERTIEGDRMARQKLDAQPWGPRKAGIKLGKHSAWLAFSLVT
                     GFTFVGYFTPIRELLSDMQHWQLGGWSCFWLGFYSLATYGNAGFLREQVCKHMCPYAR
                     FQSAMFDNHTLIVAYDSARGEPRGGRSKNQDYQAQGLGSCIDCNICVQVCPVGIDIRN
                     GLQYECISCGLCIDACNDVMDKMQYPRDLIRFSAAGQSSQHKLSQHLLRPRVLIYSGL
                     FVFMVAWLGYGLLHKPDFKVDVMRDRNIMYRETAHGVENLYQLHLTNATEQTQHYRVH
                     ASGLTGLSVESEQTLTAGPTEEVLLPFSLSLPSSEQRGSQRIKIEVREISSGETVSTD
                     STFYLP"
     gene            complement(1088..2485)
                     /locus_tag="VNZ84_04645"
     CDS             complement(1088..2485)
                     /locus_tag="VNZ84_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HWU66276.1"
                     /translation="MLPSVTFLDLETTGATPLKDRITEIALVRFDDGVETVRWQTLVN
                     PQQPIPPFIQSLTGINDAMVADAPTFAEVADKLLALLEGSVLAAHNVRFDHGFLKSEL
                     KRLGVCFRQKVLCTVKLSRLLYPQYYSHGIDAIVERHRITGLARHRAMGDVEAILIML
                     NDARRDLGEDAVRAAALKLMSAPTLPPHIDASLIGELPEAPGVYLFYGENDLPLYIGK
                     SINLRARVLSHFSSDHASSKEMRMTQEMKRFEWIPTAGEFSALLLESRLVKERLPIYN
                     RQLRRERQLCSWRLASGDTPQPLLTLVHEDEIDPSQLMQLFGTFKSKRQAVETLRKIA
                     EMSHLCVKALGLEPGRNGPCFAFQLKRCKGVCCGQESAEMHALRVQQALMAHKLKVWP
                     YPGKIGIEEYNLENDLRQIHVFAHWVYMGVAEDDDSLQALREQKTALKFDLDTYKLLL
                     KMLAQKKTRVIDLAG"
BASE COUNT          691 a          740 c          664 g          639 t
ORIGIN      
        1 ttgtggatgg agcgcaccat agaaggcgac cgcatggcaa ggcagaaact ggatgcccaa
       61 ccctggggcc cacgcaaagc tggcatcaaa ctcggcaaac acagcgcatg gctagcgttt
      121 tcgctcgtca caggctttac cttcgtcggt tacttcacgc ccatccgtga actgctcagc
      181 gacatgcagc actggcaact gggtgggtgg tcgtgtttct ggctaggatt ttacagcctg
      241 gccacctatg gcaatgcggg ttttttacgt gaacaagtct gcaaacatat gtgtccttat
      301 gcccgcttcc aaagcgccat gtttgataat cacactctga ttgtggctta tgacagtgcc
      361 cgtggagaac cacgtggtgg ccgtagtaaa aaccaggatt accaagcaca aggactgggg
      421 tcatgcatag actgcaatat ctgcgtgcag gtatgtccgg tcgggattga tatccgcaat
      481 ggcttgcaat acgaatgcat cagttgcggc ctgtgtattg atgcctgtaa tgacgtcatg
      541 gataaaatgc agtatccacg tgacctgatc cgtttctccg cagctggcca gagtagtcag
      601 cacaaattaa gccagcacct gctgcgccca cgcgtcctga tatacagtgg actatttgta
      661 ttcatggtgg cctggctggg ttacgggctg ttacataaac ccgatttcaa agtcgatgtg
      721 atgcgagacc gcaatatcat gtaccgggaa acggctcatg gcgtcgaaaa cctttaccag
      781 ctacacctga ccaatgccac agaacagaca cagcattacc gtgtacatgc cagcggcctg
      841 accggtctca gcgtagaaag tgagcaaaca ctcacggctg gcccgaccga ggaagtatta
      901 ctgccattca gtttgtcact gccctccagc gaacaacgtg gcagccagcg catcaagatc
      961 gaagtgaggg agatttcgag tggtgaaacc gtctcgacgg acagcacgtt ctacttaccc
     1021 tgattaaaag actacccctt gtcccgcgtg tgctggcagg tgattaccaa ggttgaccag
     1081 ccttagttca acctgccagg tcaatcacgc gggtcttttt ttgcgccagc attttcagca
     1141 ataacttgta agtatccaga tcgaacttga gcgctgtttt ttgctcacgc aaagcctgca
     1201 agctgtcatc atcctccgcc acccccatgt aaacccagtg tgcaaagaca tgaatctgac
     1261 gtaaatcatt ctccaggtta tattcttcaa tccctatttt gccagggtat ggccagactt
     1321 tgagtttatg cgccatcagt gcctgctgta cacgcaaggc atgcatttcg gcagattcct
     1381 ggccacagca aacgcccttg cagcgtttga gctgaaaggc aaaacacggt ccattgcgcc
     1441 cgggttccaa ccctagcgct ttgacacata aatggctcat ttcggcgatc ttgcgcaagg
     1501 tttctaccgc ctggcgcttg cttttaaacg taccaaacag ttgcatcagc tggctgggat
     1561 caatctcgtc ttcatgtaca agcgtcagca agggctgggg tgtatcgcca gacgctaacc
     1621 gccaactgca taattggcgc tctcgccgca attgccggtt atagatgggc aggcgctcct
     1681 tgaccagacg agactccagc aacaaggcac tgaactcgcc tgcggtgggg atccactcaa
     1741 aacgcttcat ttcctgcgtc atgcgcattt ccttgctgga agcatgatcg ctactaaagt
     1801 gcgatagtac gcgcgccctg agattgatac ttttaccgat atacaacggc aaatcatttt
     1861 caccgtagaa cagatagacc ccgggtgcct ctggcaattc gccgatcagg ctggcatcaa
     1921 tatgaggagg caaggtaggc gccgacatga gcttgagcgc agccgcacgc accgcgtctt
     1981 cgcccaagtc gcgccgtgca tcattaagca tgatcagaat cgcttccaca tcgcccatcg
     2041 cgcgatgacg cgccaggccg gtaatccggt ggcgctcaac aatcgcatca atgccatggc
     2101 tgtaatattg cggatacaac aggcgcgaca gtttgacggt gcacaggacc ttctgacgaa
     2161 aacaaacccc tagccgcttg agttcagact tgaggaagcc atggtcaaaa cgtacattat
     2221 gtgcggccag caccgaacct tccagcaagg ccagcaactt gtctgccacc tcagcaaacg
     2281 tcggcgcatc ggcaaccatg gcatcgttaa tgccagtcag tgactgaata aagggaggaa
     2341 taggctgctg cggattgacc aatgtttgcc aacgcacggt ttccacgcca tcatcgaaac
     2401 ggaccagtgc aatttcggta atgcggtctt ttaaaggcgt tgccccggtc gtttcgaggt
     2461 caagaaaagt cacggaaggc aacatcacgc gtaaaggctt tctactgcac atcaagttag
     2521 ctggaatgga tactatttta acgcacttca gtctttccac gatcaccagc attccgcgcc
     2581 ggaattcctg attaagcaac aggaaaagct atttaatcac ttaatcttat gtgtgatgtc
     2641 tcatatctta tacaaaacca gagtttctcc tacaaaacag ctatagacac tccaatcaat
     2701 tgatgctgta attaccaatt acatattaat gaca
//
LOCUS       DATMSK010000136         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_409507,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000136 DATMSK010000000
VERSION     DATMSK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409507"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VNZ84_04650"
     CDS             complement(<1..221)
                     /locus_tag="VNZ84_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HWU66277.1"
                     /translation="MENVSSTKPYLIRAVHEWCVDNGFTPHLLVAVDENTRVPMAYVK
                     NGEIVLNINYSATKDLVIDNTAISFSARFS"
     gene            complement(226..825)
                     /locus_tag="VNZ84_04655"
     CDS             complement(226..825)
                     /locus_tag="VNZ84_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWU66278.1"
                     /translation="MMTLYSGTTDPYSHRCRIVLFEKGMDFQVIDVDLANKPEDLAVI
                     NPHNEVPVLVERDLVLQQANIINEYIDERFPHPQLMPADPVMRARARLFLHNFEEQLF
                     SHIKDIESGNQKLADKARLIIRDNLTQIVPLFSRQEYILGDEFSMLDVAIAPLLWRLG
                     HYGIELPKQAAPLLKYAERIFSRPAYIEAMTPSEKAMRK"
     gene            complement(894..1628)
                     /locus_tag="VNZ84_04660"
     CDS             complement(894..1628)
                     /locus_tag="VNZ84_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HWU66279.1"
                     /translation="MKKVLLLVGLLLPALVMANESVHLDHAPIDPNNQASLQRGARTF
                     VNYCLSCHSAAYMRYNRLKDIGLSDAEIKSNLMFAGEKVGDTMTVTMPKRDAKSWFGA
                     TPPDLSVQARAYANGPDWLYSYLRGFYRDDTRPTGWNNTVYDKVAMPHVLWQLQGEQT
                     LKVQKTVDEHGHEVEKHTLVLTKPGVLEPAQYDAMVADLVNYLVFMSEPAKGLRLQLG
                     LIVILFLGLLFIVAYYLKKEFWKDIH"
     gene            complement(1628..2911)
                     /locus_tag="VNZ84_04665"
     CDS             complement(1628..2911)
                     /locus_tag="VNZ84_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HWU66280.1"
                     /translation="MQKYLTGLLGWVDDRFPLTANWKAHLSEYYAPKNFNFWYFFGSL
                     ALLVLVLQIVTGIFLTMNYKPDAELAFASVEYIMRDVSWGWLIRYMHSTGASMFFVVV
                     YLHMFRGLIYGSYRNPRELIWLFGVGIFLALMGEAFFGYLLPWGQMSYWGAQVIINLF
                     TSIPFVGPDISLWLRGDYMVSDATLNRFFAFHVIALPLVLLGLVAAHIIALHEVGSNN
                     PDGVEIKKHKDPETGIPLDGIPFHPYYTVKDIVGVSVFLIVFSAIIFFAPEMGGYFLE
                     ANNFIPADPLKTPAHIAPVWYFTPYYSILRAVPPIMNSQFPGVAAMGLSVVIFALLPW
                     LDRSPVKSIRYRGPIYKVWLALFVVSFLILGYLGTEPSNVWGVFDPSTFIVGGADRAT
                     VVARIFTAVYFLFFILMPWYTSIDKTKPVPERVTG"
     gene            complement(2914..3510)
                     /gene="petA"
                     /locus_tag="VNZ84_04670"
     CDS             complement(2914..3510)
                     /gene="petA"
                     /locus_tag="VNZ84_04670"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HWU66281.1"
                     /translation="MANQQVDQGKRRFLIAATTAVGGVAAAAVAVPFITSMLPSERAK
                     AAGAPVEADISKIESGAMITVEWRGKPVWLINRSQEMMAALTQHDDKLSDPKLEVTTQ
                     QPAYCQNASRSIKSNLMVLVGICTHLGCSPSPKLQAGNEMGADWPGGFFCPCHGSRFD
                     LAGRVFKGSPAPINLVVPPHKYLNDNLLLIGEDDKGAA"
BASE COUNT         1027 a          963 c          864 g          842 t
ORIGIN      
        1 gaaaatcttg ctgaaaaact gattgcagtg ttatcaatca ccaagtcctt ggtggctgaa
       61 taattgatat ttaacactat ttcgccattc tttacgtatg ccattggcac tcgggtattt
      121 tcatccaccg ccactagcaa atgtggcgta aatccattgt ccacgcacca ctcatgcacg
      181 gcgcgtatca ggtagggctt agtggatgaa acattttcca tgaccttact tccgcattgc
      241 tttttcggaa ggggtcatcg cctcgatgta agccgggcga gagaatatac gttctgcgta
      301 cttaagtaaa ggtgcagctt gctttggcag ctcaattcca taatgtccaa gacgccataa
      361 gagcggtgcg atagccacat ccagcatgga aaattcgtcg cccagaatat attcctggcg
      421 gctgaacaaa ggcacgatct gtgtcagatt gtctcgaata atcaagcgcg ccttatcagc
      481 cagcttctga ttgccagact caatatcttt gatatgacta aacaactgct cttcaaagtt
      541 gtgaagaaac aatctggcac gtgctcgcat gacagggtca gcgggcataa gctgaggatg
      601 aggaaaacgc tcgtcgatat attcattgat gatgtttgct tgctgcagca ccaaatcacg
      661 ttcaacgagg acagggactt cattgtgcgg attgattacg gccagatctt ctggcttgtt
      721 ggcaagatcc acgtcgatca cttggaaatc catgcctttt tcaaatagca caatgcggca
      781 gcggtggctg tatggatcag tagttccaga atacaaagtc atcatgatgt tgtttcctta
      841 caaaacaaaa aagcgggatt gcccgcaaag gcaatcccgc cttgaataga acgttagtgt
      901 atatctttcc agaattcttt tttcagatag tacgcgacga tgaaaagcaa gcccaggaac
      961 aggatcacga ttaatcccaa ttgcaggcgc agacctttag caggctcgct catgaatacc
     1021 aggtaattca ccaggtcagc gaccatcgcg tcatactgag caggctcaag cacgcctggt
     1081 ttggtcagca ccagcgtatg cttctcgacc tcatggccgt gctcatccac tgttttctga
     1141 accttgaggg tttgctcacc ttgcaactgc catagcacgt gcggcatagc cactttgtca
     1201 tagacagtat tgttccagcc tgtcggacga gtatcgtcac gatagaagcc tctcaagtag
     1261 ctgtacagcc agtcaggtcc gtttgcatat gcacgcgcct gcaccgaaag atccgggggg
     1321 gttgctccaa accaggattt ggcatcgcgc ttaggcatgg tgacagtcat cgtgtcgccc
     1381 accttctcac cagcaaacat caggtttgat ttgatttcag catcgctcaa accaatatct
     1441 ttaagccggt tataccgcat ataggcagcg ctgtgacagc tcaggcagta gtttacaaat
     1501 gtcctggcac cacgctgcag cgaagcctga ttattgggat cgataggcgc atgatcaaga
     1561 tgcacgctct catttgccat caccaaggca ggcaataaca ggccgaccaa caataatacc
     1621 tttttcatta tcctgttacc ctttctggaa ctggcttggt cttatcgatg ctggtatacc
     1681 aaggcatcaa gataaagaac agaaagtaaa ctgcagtgaa aattctagcc accaccgttg
     1741 cacggtccgc accgcccacg atgaatgttg atggatcaaa caccccccag acattcgaag
     1801 gctcggtacc caggtaaccc agaatcaaaa agctcactac aaacaatgcc agccacacct
     1861 tgtagatagg accgcgatag cgtatcgact tcacgggact acgatccagc caaggcagca
     1921 atgcgaaaat cacgacggac agacccatgg cagcaacgcc agggaactgc gaattcatga
     1981 tgggcggcac cgcacgcaaa atagagtaat aaggcgtgaa ataccaaacg ggcgcaatgt
     2041 gcgctggtgt ttttaacggg tcagccggaa tgaagttatt tgcttccagg aagtagccgc
     2101 ccatttccgg cgcaaagaag ataatggccg aaaacacgat caggaaaact gacacgccga
     2161 caatatcctt caccgtgtaa tacggatgaa aaggaatgcc gtccagtggg ataccggttt
     2221 caggatcttt gtgcttcttg atttcaacac catccgggtt gtttgagccc acttcatgca
     2281 gggcaatgat atgcgcagct accaatccca gcagcaccaa cggaagagca attacgtgga
     2341 acgcgaagaa ccggttaagg gtcgcgtcag acaccatgta atcaccgcgc agccaaagtg
     2401 aaatatctgg cccaacaaac gggatagagg taaacagatt aataatcacc tgagcacccc
     2461 agtaggacat ctgcccccat ggcaacagat agccaaagaa tgcctcaccc atcagcgcaa
     2521 ggaaaatccc tacaccgaac agccatatca gctcacgagg attccgataa gacccgtaga
     2581 tcagaccacg gaacatatgc agatagacca ccacgaagaa catggaagcc ccggttgagt
     2641 gcatgtagcg aatcaaccag ccccaggaaa cgtcacgcat gatgtactcc accgaagcaa
     2701 aggcgagctc tgcatcaggc ttgtaattca tcgtgaggaa aatgccagta acgatctgca
     2761 gcaccagcac caacagcgcc aatgaaccga agaaatacca gaagttgaag ttcttgggag
     2821 cgtaatactc tgagaggtga gctttccagt tggccgtcaa tggaaaacgg tcatctaccc
     2881 acccgagcaa accagttaaa tatttttgca tgattacgcg gcccccttgt cgtcttcacc
     2941 aatcaacagc agattgtcgt tgaggtattt gtgtggaggc accaccagat tgataggcgc
     3001 gggagagcct ttgaatacac gacccgccag atcaaaacgg gaaccatggc aaggacagaa
     3061 aaaaccgccc ggccaatctg cgcccatttc gttgccagct tgcaatttgg gggatggtga
     3121 acagcccagg tgagtacata tacccaccag caccatcaaa ttcgacttga tcgaacgtga
     3181 agcattctgg caataagcag gctgctgcgt ggtgacttcc agcttgggat cagacaactt
     3241 gtcatcatgc tgggtcaatg cggccatcat ttcctggcta cgattgatca accatactgg
     3301 cttaccgcgc cactcgacgg tgatcatggc gcccgactcg atcttgctga tgtcagcctc
     3361 gactggagca cccgctgcct ttgctcgctc gcttggcaac atacttgtaa taaaaggaac
     3421 tgcaacagcc gcggctgcca cacctcccac tgcggtagtc gctgcgatta aaaagcgccg
     3481 cttcccctga tccacttgtt gattcgccat cacattccct taaatttaat gcgacttgct
     3541 ctaatacagt taattagcgc gccattttac agcgttcaga gaaaaactta aacctaaaac
     3601 cgcggcttat acagggttat tgatttcgat gtagtgatgc tccagattga atcgattttg
     3661 gagatgtgcg cccaaggcct gaatgccata acgctc
//
LOCUS       DATMSK010000137         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_80185,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000137 DATMSK010000000
VERSION     DATMSK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80185"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VNZ84_04675"
     CDS             complement(<1..916)
                     /locus_tag="VNZ84_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWU66282.1"
                     /translation="MLPSATVGLLFGVWLFQQQPRLWSLTVWMVWLAVLAIPVLCRVC
                     GPVPGISFSFPSPKLLRKLWLILLVAMLGFAWAQARAWWRMHSALPVACEQQVIPVQG
                     VIVSVPERDARGQHVDVAVEQSFNPSCPLPAKVRLHLYESSHRTQPAQSSALMPRLQA
                     GERWQLSVRLKRPHATRNPHGFDYAAWCLANQIGADGSIVAKLPMVRLQSLAWQPNAL
                     IARWRSRVGDRIERVLGNTPTSAVIRALVIGEDSQITRADWQLFVDTGINHLVSISGL
                     HITMLASLGYFCAGGLWRLRPNLALYLPS"
     gene            complement(926..1633)
                     /gene="lolD"
                     /locus_tag="VNZ84_04680"
     CDS             complement(926..1633)
                     /gene="lolD"
                     /locus_tag="VNZ84_04680"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HWU66283.1"
                     /translation="MSEWILQCQGLAKTYQGLGTSVLTGVDLTLAAGEHVAIVGASGS
                     GKSTLLHLLGGLDAPTQGEVQWLGKPLTSLSERDKSAMRNQYLGFVYQFHHLLPEFSA
                     LENVAMPLLIRRLPRAEALAQAEHYLSLVGLAHRLEHTPGELSGGERQRAAMARALVT
                     QPRCVLADEPTGNLDRQTAEQVFELLLQIQSQQGTALVVVTHDLSLAARMHRQYRLQD
                     GRLQPLTSVPAQDAISR"
     gene            complement(1626..2930)
                     /locus_tag="VNZ84_04685"
     CDS             complement(1626..2930)
                     /locus_tag="VNZ84_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HWU66284.1"
                     /translation="MKNTLSALAQSLVQRLPYEYWIGWRYTRAKRNNHFISFISLTSM
                     VGIALGVMALIVVLSVMNGFQDELRKRILGVASHIEVRSFDVGLPGWQSLQQQIESIP
                     PKSVASPIEGVAPYIMAQGMLTHDQAVQGVLIRGVLPAQESKVSELSSQMKLGQLRDL
                     QPGQFNIVLGSDLAMALGVHVGEKVVLMAPQGQVTPAGLVPRIKQFTVAGIFQVGMYE
                     YDAGLALIHLDDAAKLYRLGDQVSGLRLKLADLFMADRVSRELIQALGPNYYITDWTQ
                     QHANFFKAIQLEKRVMFIILALIVAVAAFNIVSTLVMAVTDKRADIAILRTMGATPQA
                     IRKMFVVQGAMIGIIGTVSGAVLGVILALNIETVVPWIERTFNVQFLSKDVYYISDLP
                     SKLMWSDVTAIVGLSILLSLVATLYPSARAASMNPAEALRYE"
     gene            3122..3325
                     /locus_tag="VNZ84_04690"
     CDS             3122..3325
                     /locus_tag="VNZ84_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWU66285.1"
                     /translation="MNKDIFEGNWKQLKGKVQEQWGKLTNDDLDVIEGKRDQVAGKIQ
                     ERYGITKDEAEKQIEAWESIHRH"
BASE COUNT          920 a          945 c          812 g          717 t
ORIGIN      
        1 tggatggtaa atatagtgca aggtttggcc gcaaacgcca caatccacct gcgcaaaaat
       61 agcctaacga ggcaagcatg gtgatatgca aaccagatat tgagacgaga tgattaatcc
      121 cggtatcaac aaacagttgc cagtcggccc gtgtgatctg gctatcctcg ccaatgacca
      181 gtgcgcgtat gacagcactt gtaggcgtat tccccaacac gcgctcaatc cggtctccta
      241 ctctggagcg ccaacgtgca atcaatgcgt ttggctgcca ggcaagtgac tgcagccgga
      301 ccataggcaa tttggccaca atcgatccat cggcacctat ttgattggca agacaccagg
      361 ctgcgtaatc aaagccatgc gggttacgtg ttgcatgcgg acgtttgagc cggacactta
      421 attgccagcg ctcacctgct tgcaagcggg gcatcagggc tgatgattgc gctggttgcg
      481 tccgatggga agactcatac aagtggaggc gcacttttgc gggtagcgga caggatggat
      541 taaagctttg ctcaaccgcc acatccacat gctgcccacg tgcatcacgc tcagggacac
      601 tgacaatcac accttgtaca ggaatcactt gctgttcgca cgcaacaggc aaagctgaat
      661 gcattcgcca ccaggcgcgg gcctgcgccc aggcaaagcc cagcatcgca accagtaaaa
      721 tcagccacag tttgcgtagc agtttagggg aagggaagga gaaggaaata cccggtactg
      781 ggccgcaaac tcggcataga accgggatag ccaataccgc cagccacacc atccagaccg
      841 ttaaagacca taaccgaggt tgctgctgaa acagccaaac cccaaacagc aatcccactg
      901 tggcgcttgg cagcatacac ttgctttatc gtgaaatagc gtcctgggca gggacggatg
      961 ttaagggttg caacctgcca tcctgcaagc gatactggcg gtgcatgcgt gccgccaggg
     1021 acaaatcatg tgtcaccact acgagtgcgg tgccttgttg agactggatc tgcaacaaca
     1081 actcaaacac ctgctcagcc gtttgccggt ccaggtttcc tgtcggctca tccgccagca
     1141 cacatcgtgg ctgcgtgacc aatgcacgtg ccattgccgc acgttggcgc tccccgcccg
     1201 ataactcacc cggcgtatgt tccagccggt gcgccaagcc aaccagcgat aagtaatgct
     1261 cagcttgcgc caatgcctca gcacgcggca aacgtcgtat cagcaacggc atggcgacat
     1321 tttccagcgc gctaaattct ggtagcaaat ggtggaactg gtagacaaag cccagatact
     1381 ggttacgcat cgcactttta tcgcgctcag acaggctggt caatggttta cctagccact
     1441 gcacttcccc ctgggtcggc gcatcgaggc cgcctaacag gtgcagcaag gtacttttac
     1501 cactgccaga agcgccaaca attgccacat gctccccggc tgccagcgtc aaatccacac
     1561 cggttaacac cgatgtaccc aagccctgat aggtttttgc cagcccctgg cattgcaata
     1621 tccactcact catagcgcaa ggcctccgcc ggattcatac tggctgcacg cgcgctagga
     1681 tacaaggtag cgaccaggct taataaaatt gacaatccaa caatcgccgt cacatcagac
     1741 cacatgagtt tggaaggcaa atcgctgatg taatagacat ctttggacaa gaactgcaca
     1801 ttgaaagtac gctctatcca tggcacgacc gtttcaatat tcaatgccag tatcactcct
     1861 agcacagcac cagatacggt cccaataatg cctatcatgg cgccttgcac tacaaacatt
     1921 tttcggattg cctggggagt agcgcccatg gtacgcagaa tcgcgatatc cgcgcgcttg
     1981 tcagtcactg ccatcaccag cgtagacaca atattgaaag ccgccacggc tacgattagc
     2041 gcaagaataa taaacatcac acgcttttca agctgaatcg ctttgaaaaa attggcatgc
     2101 tgctgtgtcc aatcggtaat gtagtaatta ggccccaagg cctggatcaa ctcacggctc
     2161 acacggtctg ccataaacag gtcggccagt ttcaggcgca ggccagagac ttgatcgccc
     2221 aagcggtaga gttttgcagc atcatcgaga tggatgagag ccaagccagc gtcatattca
     2281 tacataccca cctgaaaaat accggccaca gtaaattgtt tgatacgtgg gaccaatccg
     2341 gcgggcgtca cctgcccttg tggcgccatc agcaccactt tttcacccac atgcaccccg
     2401 agcgccatcg ccaggtctga cccaagcaca atattgaact gccctggctg taaatccctt
     2461 aactggccca gtttcatctg gctagaaagc tcagacactt tactttcctg ggcgggtaac
     2521 acgcctcgta tcagtacgcc ttgtacagcc tggtcatgcg tcaacatgcc ttgcgccatg
     2581 atatagggcg cgacgccttc aatcgggctg gccacagact taggcggaat agactcaatc
     2641 tgttgttgca agctttgcca gcccggcaag cccacatcaa aactgcgtac ttcgatatgc
     2701 gaagctacgc ccagaatgcg cttgcgcaat tcatcctgaa atccattcat gactgacaaa
     2761 accacaatca aggccatcac accgagtgca atccctacca tgctggtcaa tgaaataaag
     2821 gagatgaaat ggttattgcg cttggcacgt gtatagcgcc agcctatcca gtattcataa
     2881 ggcaaacgtt gcaccagtga ttgggctaac gcagaaagtg tatttttcat gcccgaatct
     2941 taacagcttt taatatgaaa gcggaaccgt gcccgagcaa aacgcttcaa caaactctgg
     3001 ttgtcagcct aatctgacct aaagttagtc caatggctga ctgaacacct catcatttct
     3061 ctttatcatg caacacaagg tcaacgacct gattacttaa atcaattgag gagaactcga
     3121 catgaataaa gatatcttcg aaggtaactg gaaacaactc aaaggcaaag tccaggaaca
     3181 atggggcaag ttaaccaacg atgatctgga tgtgattgaa ggcaaacgcg accaagtggc
     3241 cggtaaaatc caggaacgtt atggcatcac taaagatgag gctgaaaagc aaatcgaagc
     3301 ctgggaaagc atacacagac actaattgtg gtctgtgagt aaaaaaggac aggcgaggct
     3361 tgtccttttt taatggcgag ttaccccatc aacc
//
LOCUS       DATMSK010000138         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_221553,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000138 DATMSK010000000
VERSION     DATMSK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221553"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VNZ84_04695"
     CDS             complement(<1..951)
                     /locus_tag="VNZ84_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWU66286.1"
                     /translation="MKLTFMGATGTVTGSKYLLESGGQQIMIDCGLFQGLKQLRLKNW
                     VPLPVDASSIHSVILTHAHIDHSGYLPLLVKHGFSGNIYCSHATADLCKILLPDSAHL
                     QEEEAAFANRRGFSKHKPALPLYTQEDAAETIKLLTPVEMEREIELGSDLTLTLYPSG
                     HILGASFVQIRHAKKTIVFSGDIGRSNDVLMHPPASIKHADYLVVESTYGNRLHSLED
                     PKIKLANIIQHAYKRKGVIVVPVFAVGRAQELLYYIHMLKASGSIPEDIPVYLNSPMA
                     VEATEIYHHYSTQHRLNQSQTEVLFRTAHLVNSIEESKRLN"
     gene            1136..3682
                     /gene="mutS"
                     /locus_tag="VNZ84_04700"
     CDS             1136..3682
                     /gene="mutS"
                     /locus_tag="VNZ84_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWU66287.1"
                     /translation="MSFENHTPMMRQYLTLKAQYPDMLLFYCMGDFYELFFEDAEKAA
                     RLLGITLTRRGASNGEPIKMAGLPYHAVEGYLAKLAKMGEAVAICEQVGDPATSKGPV
                     AREVTRILTPGTLTDAALLDETRDNTLLCVAKGEGVLGLARLNLASGEFVLTEIAPGH
                     LAQELERIHPAELVVAEDHTSEVLQQARCAKKRLAPWQFDLDTAQRSLTQQFNTHDLA
                     GFGCGEMPAAVTAAGALLEYVKHTQRAALPHIAGLRVEQSSHYLQLDAATRRNLEIDT
                     TIRGETTPTLYSLLNSTQTAMGARLLRHWLHHPLRSQHTIQQRHLAVAELFEQFLDSA
                     LQQALHPIGDIERMTARVALRSAKPRDLSGLRDSLLQLPALVATLPGVTSSLLQAELA
                     ELQAPSDALLLLTQAIQVEPSAILREGGVIEKGYDAELDELRAIQTDHGDFLLQFEAA
                     EKARTGITNLKVEYNSVHGFYIEISRLQAESAPPEYRRRQTLKNVERFITPELKAFED
                     KVLSANERALAREKMLYEQVLDALLPHITTLQRIATAVATLDVLACFAERARALNYVQ
                     PEFRADIGLNIQHGRHPVVESIAQPYVANDVLLNPYRQLLLITGPNMGGKSTFMRQTA
                     LIVLLAYCGSFVPATSAVIGEIDRIFTRIGASDDLAGGRSTFMVEMTETANILNNATP
                     NSLVLLDEIGRGTSTFDGLSLAWAVAKQLLEKNKSLTLFATHYFELTRIVDEAKHAAN
                     VHLDAVEHGQGIVFLHKVEEGAATMSYGIQVAQLAGIPKLVVQLAKRKLQQLENQTIT
                     ATPQNDLFASHPEPEEPPLHPLVAELERIQPDDLTPKQALELLYSLKKQL"
     gene            3693..4286
                     /locus_tag="VNZ84_04705"
     CDS             3693..4286
                     /locus_tag="VNZ84_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWU66288.1"
                     /translation="MQKPAITQQPIHELIANRWSGRAYDASKPVSKEQVVSLLEAARW
                     APSCFGDQPWRYVFCNKANNPSAWQAAFDCLVPGNQSWAVNAPVLLLICADTLFGHND
                     KPNKWAAYDTGAATENLCLQATAMGLMAHQMGGFDADKARNTFNVPERYQILAMVTVG
                     YQAAVETLSGEAKERELAERSRKPLEDLFFNGIWQGK"
BASE COUNT         1127 a         1017 c         1174 g         1124 t
ORIGIN      
        1 atttaagcgt ttggattctt ctatgctgtt caccagatga gccgtgcgga acaacacttc
       61 tgtttgcgat tggttcaagc gatgctgtgt ggagtaatga tggtaaatct ccgtcgcttc
      121 gacggccatg gggctattca ggtagacggg gatatcctcc ggaatgctgc ctgaagcttt
      181 gagcatatga atgtagtaga gcaattcctg cgcccgccca acggcaaata ccggtacaac
      241 aatgacgcct ttacgcttat aagcatgctg gataatattg gccagtttta ttttgggatc
      301 ctccaagctg tgaaggcgat taccataagt ggactccacc acaagatagt ccgcatgctt
      361 gatggaggca ggcgggtgca tcagtacatc atttgaccgt ccgatatcgc cggaaaaaac
      421 aatggttttc tttgcatgcc ggatttgcac aaaacttgca cccagaatgt ggccgcttgg
      481 atacagggta agtgtgagat ctgagcccaa ctcaatttcc cgttccattt caaccggggt
      541 gagaagtttg atggtttcag ccgcatcttc ctgggtataa agcgggagtg cgggtttgtg
      601 tttggaaaag ccacgccggt tcgcgaatgc agcctcttct tcttgcaggt gggctgaatc
      661 tggaagtaat atcttgcata aatctgcagt ggcgtggctg cagtagatgt tgccggaaaa
      721 accatgtttt accagcaaag gcaggtaacc gctgtggtca atatgggcat gtgtgaggat
      781 aacgctatgg atgctgcttg catccactgg caagggtacc cagttcttga gccgcaattg
      841 cttgaggccc tggaataagc cacaatcaat catgatctgc tggcctcccg attcaagcaa
      901 gtatttggaa ccggtgacgg tccccgtggc ccccatgaac gttaacttca tcagcatctc
      961 cccctatagc tattttttat cagctcacgc gatgaaccaa gtataaaagc atactgcaat
     1021 cacatattga tgtaacgcat ataaagcaca atggtgatgc gtgctgcatt tgttgtaaga
     1081 tgctggagtt ttgctaaatt cactttcatt atcatcaacg ttctgaaata gccgcatgtc
     1141 cttcgaaaac cacaccccca tgatgcgtca atacctgacg cttaaagcac agtatccaga
     1201 tatgctgctg ttttactgca tgggtgattt ttatgaattg ttttttgagg atgcagaaaa
     1261 agcagcacga ttgctgggta tcacattaac ccgtcgtggt gcgagtaatg gcgaaccaat
     1321 aaaaatggcg ggcttacctt accatgcggt cgaaggctat ctggccaagc tcgccaaaat
     1381 gggtgaggcg gtggcaattt gcgagcaggt gggcgatcca gccacttcaa aagggccagt
     1441 cgcgcgcgaa gtgacgcgta ttcttacgcc aggtactttg accgatgctg cgttgctgga
     1501 tgaaacccgt gacaatactt tgctgtgtgt cgccaaaggg gagggtgtgc tggggttggc
     1561 gcggctgaat ctggcatcag gcgaatttgt gctgactgaa attgcgccag gacacctggc
     1621 ccaagagctg gaacgtatcc atcctgcaga gttggtggtg gctgaggatc atacttcaga
     1681 ggtgttgcag caagctcgat gtgccaaaaa gcgcttggca ccatggcagt ttgacctgga
     1741 tacggcgcag cgcagtttaa cgcaacagtt taatacgcat gatctggcag gattcggttg
     1801 tggcgaaatg cctgcggcgg tgacggcagc aggcgcattg ctggaatatg tgaagcatac
     1861 gcaacgagcg gccttaccgc atattgccgg cttgcgtgtg gagcagagca gtcactacct
     1921 acaactggat gcagcgacac ggcgcaacct ggaaattgat acgactatcc gtggtgaaac
     1981 gacgccaacg ttgtattcac tgctgaactc gacacaaacg gcgatgggtg cccgtttgct
     2041 acggcattgg ttacatcatc ccttgcgtag tcagcacacc atacagcagc gccatttagc
     2101 agtggctgaa ctgttcgaac aatttctaga tagtgcatta cagcaggctt tgcatcccat
     2161 cggcgatatt gagcgcatga ctgcacgcgt ggcgctcagg tccgccaagc cgcgtgattt
     2221 gtcgggcttg cgagatagtt tattgcaatt gccggcattg gttgcgacat tgccaggcgt
     2281 tacatcctcg ttattgcaag ctgagttagc agagttgcag gcgccttctg atgctttgct
     2341 tttgttaacg caggcgattc aggtcgagcc ttctgccatc ttgcgtgaag gcggtgtgat
     2401 tgaaaaaggc tatgatgccg agttggatga gttgcgtgcg attcagaccg atcatggcga
     2461 ttttttgctg caattcgagg cagctgaaaa agcacgtaca ggtattacca acctcaaggt
     2521 tgaatacaat agcgtacatg gcttttatat tgaaatcagc cgcttgcagg cagagagtgc
     2581 tccgccggaa tatcgccgcc gccaaacctt gaaaaacgta gagcgcttta ttacacctga
     2641 gttaaaagcg tttgaggata aagttctaag tgccaatgag cgggcactgg cacgcgaaaa
     2701 aatgctgtac gagcaggtgc tggatgcgtt gctaccacat atcactactt tgcaacgtat
     2761 cgccaccgca gtggcaaccc tggatgtctt ggcttgcttt gctgagcggg cacgggcatt
     2821 gaactatgtg cagcctgaat ttcgcgccga tattggcctc aacattcagc acggccgcca
     2881 tccggtggtc gaaagtatcg cccagccata tgtggcgaat gatgtgctcc tgaatcctta
     2941 ccgccaatta ttgctgatta ccggtcctaa tatgggcggt aagtcgacct ttatgcggca
     3001 aacggcattg attgtcttgc tggcgtattg tggcagtttt gtgccagcga caagtgccgt
     3061 gattggcgag attgaccgta tttttaccag aattggcgcc tctgatgatc tggccggcgg
     3121 ccgttcaact tttatggtgg aaatgaccga aaccgccaat atcctcaata atgcgactcc
     3181 caatagtctg gtgctgctgg atgaaatcgg tcgcggaacc agcacatttg acggcctcag
     3241 cctggcttgg gcggtggcta aacagttgct ggaaaaaaac aaatcactga ccctgtttgc
     3301 tacacattat tttgaactga cacggattgt tgacgaagcc aaacatgcgg ccaacgtgca
     3361 tttggatgcc gtagagcatg gtcagggaat tgtcttcctg cataaggttg aagagggtgc
     3421 agccaccatg agttatggta tccaggtcgc acagctggca ggtataccaa agttagtggt
     3481 gcaattagcc aaacgaaagc tacaacaact cgaaaaccag accatcacag cgacaccgca
     3541 aaatgatttg tttgccagcc atccagagcc agaggaaccg ccattacatc cattggtcgc
     3601 agaattagag cgtatacagc ctgatgactt gacacccaag caggctctgg agctactcta
     3661 ttcattgaaa aaacaactat gaggattcaa tcatgcaaaa accagcgata acacagcaac
     3721 ccattcatga actgattgcc aaccgttgga gcggccgtgc ttatgatgca agcaaaccag
     3781 tcagtaaaga gcaagtagtc agcttgctag aagctgctcg gtgggcacca tcttgctttg
     3841 gtgaccagcc atggcggtat gtattctgca acaaggcaaa taacccctcc gcttggcaag
     3901 ctgcatttga ttgcttggtt cctggtaacc aaagttgggc tgttaatgcg ccagtattgc
     3961 tgttaatttg tgcggatacc ttgtttggac acaatgataa gcccaataaa tgggctgctt
     4021 atgacacagg cgcggctact gagaatttat gtttgcaagc tactgctatg ggcttaatgg
     4081 cgcatcaaat gggcggtttc gatgcagata aagccaggaa tacgttcaat gtgccagaac
     4141 gttaccagat tctagccatg gtaacggtcg gttaccaggc agctgtggaa accttgtctg
     4201 gtgaagctaa agagcgcgaa ttggcagagc gtagtcgtaa gccgctggaa gatttgttct
     4261 ttaacgggat ctggcaaggc aaataactta ataacattgg cccatttggg tagataggct
     4321 gctaatcagc aagattattg ttgagttatt taatttgata gattagcatg attaacaact
     4381 aaaagtttta attgaagtcc gcgaattcgg gctcaaatga ttgaggagaa gtaacatgtt
     4441 ta
//
LOCUS       DATMSK010000139         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_104633,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000139 DATMSK010000000
VERSION     DATMSK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104633"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VNZ84_04710"
     CDS             complement(<1..710)
                     /locus_tag="VNZ84_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWU66289.1"
                     /translation="MTDQSKDSKYKLNLPETSFPMRGDLSKREPAWLKSWQERKVYER
                     IRAKRKGAPAFILHDGPPYANGDIHIGHAVNKILKDIIIKAKTMSGFDAPYVPGWDCH
                     GLPIELVVEKNHGKNIDPAKFRELCRAYAAEQVEKQKKDFIRLGVLGDWDHPYLTMAF
                     NTEADIMRALGEIYQNGYLYQGSKPVHWCVDCGSALAEAEVEYEDVNSPAIDVGFKVV
                     DNAALAKAFGVDKIDGSV"
     gene            complement(762..1655)
                     /locus_tag="VNZ84_04715"
     CDS             complement(762..1655)
                     /locus_tag="VNZ84_04715"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWU66290.1"
                     /translation="MQIFRHIPHTSVPQAIAIGNFDGMHLGHQALLRQLIAFTQTYKI
                     TPAVMTFEPHPREFFMPQNAPARLASMREKLEYFVECGVQHVYVQRFNQAFAAQSPAT
                     FMQVLRQQLNANVVMVGEDFCFGAKRAGSVQTLIEHGFNVIPLPEVQLDDERVSSTLV
                     RNALAAGELVKAHALLGRPYSISGKVVHGAKLGRQLGFPTANVHMRHERPALTGVYAV
                     KLNNLPAVANLGSRPTLEGIPKLKLEVHVFDFNGDLYDQHVHVQFFHKLRDEQKFAGL
                     DALKAQIALDALKARQYFSHG"
     gene            complement(1661..3205)
                     /gene="murJ"
                     /locus_tag="VNZ84_04720"
     CDS             complement(1661..3205)
                     /gene="murJ"
                     /locus_tag="VNZ84_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWU66291.1"
                     /translation="MNLLKALATVGGMTFISRLLGFVRDTLIARIFGAGMATDAFFVA
                     FKIPNLLRRISGEGAFSQAFVPILSEYRKQRTPEATKALINHVSSLLGLFLVAITIVG
                     MLAAPLIVSLTSPGFVHDPAKFALTTELLRITFPYILFISLVSMAGGVLNAFSKFQVP
                     AFTPVWLNLSFITAALFVAPHFNNSVYVLAWAVFVGGIIQLLYQLPYLQEIGMLPRYE
                     LNLQDEGVRRILKLMGPAIFGVSVAQISIVLNNIFASFMPTGSVSWLYYADRLMEFPT
                     GVLGVALGTILLPSLSKAHVDKSSTEFSLLLDWGLRLTFMLAIPAAVAMAVLSKPLVA
                     SLFYYGKFTPADVVQTERALIAYSLSLLGLIMVKVLAPGFYARQNVKTPVKIAIFTLV
                     MTQLMNVCFLMFTSLQHAGLALAVGLGACLNATLLWVTLQRTGAFQPQPGWRLFLFKL
                     LIAVTIMALALHTAATWSGDWLAMATLNRMLHLAGVVVLGMGIYFVSLGLLGFRPKDF
                     MKRVAK"
     gene            3412..>3613
                     /gene="rpsT"
                     /locus_tag="VNZ84_04725"
     CDS             3412..>3613
                     /gene="rpsT"
                     /locus_tag="VNZ84_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWU66292.1"
                     /translation="MANTAQARKRARQSVKVNAHNAALRSTLRTAIKKVIKAIQTGDK
                     AAAVASYQENVSVIDRIADKKII"
BASE COUNT         1030 a          967 c          837 g          779 t
ORIGIN      
        1 tagacacttc catcaatttt atcgacacca aaagctttgg cgagcgccgc gttatccact
       61 actttaaagc ctacatcaat cgcaggcgaa ttaacgtctt cgtattccac ctctgcttca
      121 gccagcgcag aaccgcaatc cacacaccaa tggactggct tagagccttg gtagaggtag
      181 ccattctggt agatttcacc caaggcacgc atgatatcgg cctcggtgtt aaatgccatg
      241 gtcaggtaag gatgatccca gtcacccaac acacccagtc ggataaagtc ttttttctgc
      301 ttctcgacct gctcagccgc ataggcacgg cacagttcgc ggaacttggc cggatcgata
      361 ttcttgccat ggtttttctc aaccaccagc tcgatcggta ggccgtgaca atcccagccc
      421 ggcacataag gcgcgtcaaa accactcatg gttttggcct tgatgatgat gtctttcagg
      481 attttgttaa cggcatgacc aatgtgaata tcaccattgg cgtacggtgg gccatcatgc
      541 aagataaagg ctggcgcgcc tttacgcttg gcacggatgc gctcgtacac tttgcgctct
      601 tgccagcttt tcaaccaggc gggttcacgc ttggacaagt caccgcgcat cgggaagctg
      661 gtttcaggta aattcagttt atattttgaa tctttgcttt ggtcagtcat ttaaatatcc
      721 aaaaaaatca gccacagatg atggctgatc aatgttcagc gttatccgtg gctaaaatat
      781 tgtctcgcct tcaaggcatc cagcgcaatc tgcgctttta atgcgtccag cccggcgaac
      841 ttttgttcat cacgcagttt atgaaaaaac tgcacatgta catgctgatc ataaagatca
      901 ccattaaaat caaacacatg tacttcaagc ttgagtttgg ggatgccttc gagcgtcggc
      961 cggctaccca aattggcaac cgccggtaaa ttgttcaatt ttaccgcata aacccctgtc
     1021 agtgcaggcc gttcgtggcg catatgcaca ttggccgtgg gaaaaccaag ctggcggcca
     1081 agtttggcac catgcaccac tttaccgcta atactataag ggcgcccgag caatgcatgt
     1141 gccttgacca actctcccgc agccagcgca ttacgcacca atgtgctgga aactcgctca
     1201 tcatccagtt gcacttcagg caaaggaatc acgttaaacc catgttcaat cagcgtttgc
     1261 acactgccgg cccgcttggc gccaaagcag aaatcctcgc caaccatgac cacattggca
     1321 ttcagctgct ggcgaagtac ttgcataaat gtggccggac tttgcgctgc aaatgcctgg
     1381 ttaaagcgct gcacatagac atgctgcaca ccacactcga caaaatactc cagcttttca
     1441 cgcatggaag ccaaccgtgc tggtgcattc tgaggcataa aaaactcccg cggatgcggc
     1501 tcaaacgtca tcacggccgg ggtaatttta taggtctgcg taaaagcaat cagttggcgc
     1561 aacagggcct ggtggccgag atgcatgcca tcgaaattac caatcgcgat ggcttgcggt
     1621 actgacgtgt ggggaatatg acgaaatatc tgcataacca ttatttagcc actcgcttca
     1681 taaaatcctt aggcctgaaa cccaacaagc caagggagac aaagtaaata cccatcccca
     1741 gcaccactac tccagccaga tgtaacatgc ggttcaacgt agccatggcc agccaatcac
     1801 ctgaccaggt ggcagcggta tgcaatgcca gcgccataat ggtgaccgca atcaagagtt
     1861 taaacaggaa taatcgccag ccaggctgtg gctggaacgc gcccgttctt tgcagggtca
     1921 cccatagcag cgtcgcattc aggcaagcac ccaggccaac ggctaacgcc agccctgcat
     1981 gctgcaaaga ggtgaacatc agaaaacaca cattcattag ctgcgtcatc accagtgtaa
     2041 aaatggcaat tttgaccggc gttttgacgt tttggcgcgc ataaaaaccg ggcgcaagca
     2101 ctttcaccat aatcaagccg agcaggctca gactgtaagc aatcagcgcg cgctcggttt
     2161 gtaccacgtc agcaggggtg aacttgccat aataaaacag gcttgccacc aatggtttgg
     2221 agagcactgc cattgcaaca gcagccggaa tcgccagcat aaaggtcaga cgcaaccccc
     2281 agtctaacaa gagcgaaaac tcagtgctgg atttatcaac atgcgccttg gataagctag
     2341 gcaacaaaat ggtgcccaat gcgacaccca gtacacccgt cgggaactcc atcaggcgat
     2401 cggcataata tagccaggac acactacccg tcggcatgaa tgaggcaaag atattattca
     2461 gcacaatcga aatctgggcg acagatacgc caaagattgc tggccccatc aacttgagaa
     2521 tacggcgcac accttcatcc tgcaagttga gttcataacg cggcaacatg ccaatttcct
     2581 gcagataagg gagctgatac aacaactgaa taatgccccc gacaaatacc gcccacgcca
     2641 acacatacac cgaattatta aaatgtggcg caacaaacaa ggccgcagtg ataaaactca
     2701 ggttgagcca aactggcgta aatgcgggga cctggaattt gctgaaggca ttgaggacac
     2761 cgcccgccat ggaaaccagc gaaataaaca gaatataagg aaaggtgatg cgcagcagct
     2821 cggtggtcag tgcgaatttg gcgggatcat gcacaaagcc aggcgaagtc aggctcacaa
     2881 tcaggggcgc agccagcata ccaacaatgg taatcgcgac caggaacaaa ccaagcaaac
     2941 tcgacacatg gttaatgagt gccttggtgg cctcaggcgt acgttgcttg cggtattcgg
     3001 aaagaatcgg cacgaaagcc tggctgaatg ccccttcccc agaaatacga cgcaacaggt
     3061 taggaatttt aaatgcgaca aagaaagcat ccgttgccat ccctgcacca aaaatccgcg
     3121 caatcagcgt gtcccgtaca aaccccagca agcgcgagat aaacgtcatg ccacccacag
     3181 tggccagagc tttgagcaaa ttcataaatg atggaaaata tgtgccttgc gttgtttttg
     3241 accgcgtacg actcagtcag ccaccggcag attaacaaag ttgggcgatt ttaccatgca
     3301 gccacaacca atcgaggaaa atgcgttttt aatcttgcaa aaacaaagca aaaacagtat
     3361 gatgcacggt ttcgtattac accccttttg aaagttacag gataaacgta tatggcaaat
     3421 accgcacaag ctcgcaaacg cgcgcgccaa tccgtcaaag taaatgcgca caatgctgca
     3481 ttacgttcta ctttgcgcac tgcgattaaa aaagtaatca aagcaattca aactggtgat
     3541 aaggctgctg ctgtggcttc ttaccaggaa aacgtgagcg tgattgaccg tatcgctgac
     3601 aagaaaatca ttc
//
LOCUS       DATMSK010000140         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_104703,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000140 DATMSK010000000
VERSION     DATMSK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104703"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..447
                     /gene="raiA"
                     /locus_tag="VNZ84_04730"
     CDS             121..447
                     /gene="raiA"
                     /locus_tag="VNZ84_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWU66293.1"
                     /translation="MNLQLTGHHLEITPALRDYVSSKFTRISNHFDHVIDVKVTMSVE
                     KLAQKVEATLHVPGNDLHAACTDENMYSAIDMLTDKLDRQVLKHKEKNGDHHKSGGAV
                     KHQLSS"
     gene            610..1560
                     /gene="hprK"
                     /locus_tag="VNZ84_04735"
     CDS             610..1560
                     /gene="hprK"
                     /locus_tag="VNZ84_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HWU66294.1"
                     /translation="MAQISVADLYKQIRSKLKLKWVAGESGGQKILNSGTVTKPSLAL
                     VGHLNFVHPNRVQVMGCAEMDYLGGLSILAMQQAVSNLFSTDLAMVVVANGEKVPPVM
                     LEAAQQSQTPLFTTPMISPQLMELLSHYLAVATAETTSMHGVFMEVQGFGVLITGSAA
                     IGKSELALELISRGHRLVADDIVDFYRVSPERIEGRCPELLQDFLEVRGLGILNIRAL
                     YGDNAVKPTKPLDMMIQLELADEMKPQDLDRLSANRQTERVLDVEVSKVIIPIAAGRN
                     IAVLVEAAVRNHMLLLRGVNATKQLTQRQKQIMLRESKLR"
     gene            1577..2458
                     /gene="rapZ"
                     /locus_tag="VNZ84_04740"
     CDS             1577..2458
                     /gene="rapZ"
                     /locus_tag="VNZ84_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWU66295.1"
                     /translation="MQLVIVTGLSGSGKTIALRVFEDSGYYCIDNLPATLLPHIQNHV
                     ADRDAAKVAVSIDSRSIAIESLPDILQTLKTQGIAAHLLFLDASLETLVKRFSETRRK
                     HPLSTPNQALAESIKHERDLLSGLSNLGHHVDTSNLSANALRHHTRDWMLQIEQSYQE
                     ARPHSLVLSFISFGFKHGIPLDADFVFDVRSLPNPHYDPILRPYNGKEAPIQQFLQNE
                     PMVLEMQHDIQHYIEKWLPSFYHDHRSYVTIGIGCTGGQHRSVYLVEQLGAYFKQQHQ
                     VMTRHRELQDAPVHAAP"
     gene            2483..2890
                     /locus_tag="VNZ84_04745"
     CDS             2483..2890
                     /locus_tag="VNZ84_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWU66296.1"
                     /translation="MIGVLIIAHGSLGPSLIDCATHVMGNRPAQLDFLEISKHDDPAS
                     ILPKAQALVKKLDSGQGVLVLSDIYGATPCNIVTRLLTPDEIEGVAGVNLPMLVRVLN
                     YRHEGLHSCIQKALSGGRDGVVHFTKTTCHHAQ"
     gene            2903..3172
                     /locus_tag="VNZ84_04750"
     CDS             2903..3172
                     /locus_tag="VNZ84_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWU66297.1"
                     /translation="MAKLSLEIINKLGLHARASTKFTQTASQFKSEVWVEKNGRRVNA
                     KSIMGVMMLAAAKGSIIDIEATGPDEEQALAALRELVNNYFGEGE"
     gene            3173..>4720
                     /gene="ptsP"
                     /locus_tag="VNZ84_04755"
     CDS             3173..>4720
                     /gene="ptsP"
                     /locus_tag="VNZ84_04755"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HWU66298.1"
                     /translation="MTSFSMHGVGVSSGIAIGYAHLISHALLEVVHFKIEAKGVDDEI
                     ARFERAIDLVKKDLQQLKAQLPQNAPAELSAFINTHLAMLSDKSLSEAPKAIIRNELC
                     NAEWALKQQMDDIVAQFDAIEDAYLRERKQDVVQVVERVIKVLLGRDQLAASVKKLQA
                     RQERAMILVAHDVSPADAIQFKHHQFAAFITDVGGVTSHTAILARSLNIPSIVALQRA
                     RDLVNDGELMIVDGAAGVVIVNPDPEVLSEYQLKQNQWQLAQQKLQRIKTTKAVTVDG
                     VAIDLLANIEVPEDVISAKASGAVGVGLYRTEFLFMNRSEMPGEQEQFEAYKHVAEAM
                     KGYPVTIRTLDIGADKQLNPDEVVTATNPALGLRAVRYCLAEPHIFHTQFRALLRASH
                     YGQVKILIPMLSNLAELRQARLLLERAKESLRKQSIPFDEKIQMGGMVEVPAAAINAD
                     AFAQELDFLSIGTNDLIQYTLAIDRADDAVAHLYNPVHPAVLRLIQLTIQAAEKFQKP
                     VSVCGEMA"
BASE COUNT         1209 a         1265 c         1184 g         1062 t
ORIGIN      
        1 agcagttcac ttccgggttg catctgtttt ccaaaagagt aaactgaaat tgtcctctct
       61 atccaattga taaggcaatt ggctctggaa aggcgccatt tctgaaaaaa ggagtttgtc
      121 atgaatttac aacttactgg acatcattta gaaatcactc cggcattacg cgactacgtt
      181 tcatcgaaat ttacgcgtat cagcaatcac ttcgaccatg tgatcgatgt caaagtcacc
      241 atgtctgttg aaaagctcgc ccaaaaggtt gaagccacgc tgcatgttcc aggcaacgat
      301 ttgcacgctg cctgcaccga tgagaacatg tatagcgcca tcgacatgct tactgacaaa
      361 cttgaccgtc aggtattgaa acacaaggaa aaaaatggcg atcaccataa atccggtggc
      421 gctgtgaaac atcagttaag ttcctaagtt ttagcctcac attctctacc cgcttgggta
      481 gagaattcac ctcaaagcct ctgtatcact tttacctcgt ttcaccgcct ttacgtcaat
      541 tcacccccgg caggccacat tcagttgtaa aataagcctt aatgacttgt tgatatgtaa
      601 acaaccaata tggctcaaat ctcagtagcc gatttgtaca aacagatacg cagcaaactc
      661 aagctgaaat gggtggctgg cgagtctggt ggtcaaaaaa tcctgaattc cggcaccgtc
      721 accaagccct cgctggcttt ggtcggtcat ttgaattttg tgcaccccaa tcgcgtgcag
      781 gtcatgggtt gtgctgaaat ggactatcta gggggactta gcatcctcgc catgcaacag
      841 gcagtcagca atttattctc gaccgacctg gccatggttg tggtcgccaa tggcgaaaaa
      901 gtcccgcccg tcatgctgga agctgctcag caatcccaaa caccactatt taccacaccc
      961 atgatcagcc cgcagctcat ggagttactg agtcattacc tggccgtggc aacggcagag
     1021 acgaccagca tgcatggcgt gtttatggaa gtgcaaggct ttggggtgct aattaccggc
     1081 agtgctgcca ttggtaaaag tgaattggcc ctggagctga tttcgcgtgg ccaccggctg
     1141 gtagcagatg atattgtcga tttttaccgt gtctctccag agcgtataga aggccgctgc
     1201 ccggagcttt tacaagactt tctggaggtg cgcgggcttg gcatcctcaa tatccgcgca
     1261 ctctatggcg ataatgcagt caaaccaacc aaaccactgg acatgatgat acagctggaa
     1321 ttggcggatg aaatgaaacc acaggacctg gatcgcctga gtgcaaaccg gcagactgaa
     1381 cgtgtgctgg atgtggaagt cagcaaggtg attatcccga ttgcagccgg ccgcaacatt
     1441 gcagtgctgg tcgaggccgc cgtgcgcaac catatgctac tactacgtgg cgtcaacgcc
     1501 accaagcaac tgacgcaacg gcaaaagcaa atcatgctcc gggaaagcaa attaagatga
     1561 tgaaaaggtc gcaatcatgc agctggtgat cgttacagga ttgtcaggtt caggaaaaac
     1621 catcgcactg cgtgtgtttg aagacagcgg ttattactgt attgataacc tgcctgcgac
     1681 cctgttaccc catatccaaa accacgtcgc tgaccgtgat gccgccaagg tggcggtcag
     1741 cattgatagc cgcagcattg cgattgaatc gctccctgat atcctgcaaa ccctcaagac
     1801 ccaaggcatt gcggcccatc tgctgtttct tgatgccagc ctggaaacct tagtgaaacg
     1861 tttctcggag acgcgtcgca agcacccgct cagcaccccc aaccaggcac tggcggaaag
     1921 catcaaacac gagcgcgacc tgttatccgg actaagcaat ctgggtcatc atgtagacac
     1981 cagcaacttg tcggccaatg cgctacgtca ccatacccgt gactggatgc tacaaattga
     2041 gcaaagctac caggaggcgc gcccgcactc attggtgcta tcgtttattt cgtttggctt
     2101 caaacatggc atcccactgg atgcggattt tgtgtttgat gtacgcagcc tgcctaaccc
     2161 gcattacgac ccaattttgc gtccttacaa tggcaaagag gcgccaatcc agcaattttt
     2221 gcaaaatgaa cccatggtgc ttgaaatgca gcatgacatt cagcactata tagaaaaatg
     2281 gctaccaagc ttttatcacg accatcgcag ctacgtcacc atcggcatag gctgtaccgg
     2341 ggggcaacac cgttccgtct atctggtgga acaactgggg gcttacttca agcagcagca
     2401 tcaagtcatg acacgtcatc gtgaattgca ggatgcgcct gttcacgctg ccccttaatt
     2461 taaggactct gagagcacaa ctatgattgg tgtattgatt attgcgcatg gcagcctggg
     2521 gcccagcctg atcgattgcg caacacatgt gatgggcaac cgcccggcgc agctggattt
     2581 cctggaaatt tccaaacacg acgacccggc cagtatattg cccaaggcac aggcactggt
     2641 taaaaaactg gatagtggcc agggcgtcct ggtcctgtca gacatttatg gcgccacgcc
     2701 gtgcaatatt gtgacccggc tgctgacgcc cgatgaaatc gaaggtgtcg ccggggttaa
     2761 cctgcccatg ctggtacgcg tgcttaatta ccgtcacgaa ggcctacatt cctgcatcca
     2821 gaaagccctg agtggcggcc gcgatggcgt tgtccacttt accaaaacca cttgtcatca
     2881 cgcacaataa ctttaaaacc ctatggcgaa actctcttta gaaatcatta ataaactcgg
     2941 tttacacgca cgcgcttcga ccaaattcac ccaaaccgcc agccagttca agagcgaagt
     3001 ctgggtcgag aaaaatggtc gccgcgtcaa tgctaaaagt atcatgggcg tcatgatgct
     3061 ggcggccgcc aaaggcagca tcatcgacat tgaagcgacc gggccggatg aagagcaagc
     3121 gctcgccgca ctccgggaac tggtcaacaa ctactttggt gagggtgagt aattgaccag
     3181 cttcagcatg catggcgtgg gggtgtccag cggcatcgcc attggctatg cccacctgat
     3241 ttcccatgcc ttgttagagg tcgtccactt caagattgaa gccaagggcg tcgatgacga
     3301 aattgcccgt tttgaacgtg cgattgacct ggtcaaaaaa gacctgcaac agctcaaggc
     3361 acaactcccg caaaatgcgc ctgcagaatt gagtgcgttt attaacacgc acctggccat
     3421 gctcagcgac aaatcattgt ctgaagcgcc caaagccatt atccgtaatg agttatgcaa
     3481 tgccgagtgg gcactcaagc agcaaatgga tgatatcgtg gcccagtttg atgccattga
     3541 agatgcctat ttgcgtgagc gcaaacaaga tgtcgtacag gtcgttgaac gcgtgatcaa
     3601 ggtgttgctg ggacgggatc aactggcagc cagcgtaaag aaactgcaag ccaggcaaga
     3661 gcgcgcaatg attctggtcg cacacgacgt ctccccggcg gatgccatcc agttcaagca
     3721 ccaccagttt gcggccttta ttaccgatgt tggtggtgtg acctcgcaca cggccattct
     3781 ggcgcgtagc ctgaacattc catccatcgt tgccctgcaa cgtgcgcgcg acctggtgaa
     3841 cgatggcgaa ctcatgattg ttgatggtgc cgctggggtg gtgatcgtca atcctgaccc
     3901 cgaagtgctg tccgaatacc agctgaaaca aaaccagtgg caactggcac aacaaaaact
     3961 ccagcgtatc aaaaccacca aggcggtcac tgtcgatggc gtggccattg atttgctcgc
     4021 caatattgaa gtacctgaag acgtgatcag tgccaaagct tctggtgcgg tgggcgtggg
     4081 tttgtaccgg actgagttcc tgtttatgaa ccgcagcgaa atgccaggcg aacaggagca
     4141 atttgaggct tataaacacg tggctgaggc gatgaaaggc tacccggtga ccatccgcac
     4201 cttggacatc ggcgctgata aacaactcaa tccggacgaa gtggtcacgg cgaccaatcc
     4261 ggcattgggc ttgcgcgctg tgcgctattg cctggctgaa ccacatattt tccataccca
     4321 gttccgcgcc ctcttgcgcg catcgcatta tggccaggtc aaaatcctga tccccatgct
     4381 gtccaacctg gctgaactac gccaagccag gttattgctg gaacgcgcca aagaatcgct
     4441 acgcaaacaa agtatcccat tcgacgaaaa aatccagatg ggtggcatgg tagaagtccc
     4501 agccgcggcc attaatgccg acgcgtttgc ccaggagcta gactttttat caataggcac
     4561 taacgacctg atccagtaca cattggcgat tgaccgcgca gatgatgccg tcgcacactt
     4621 gtataacccc gtgcatccgg cggtactgcg cctgatccag ctcaccatac aggcggcaga
     4681 gaaattccag aaaccggtct cagtttgcgg cgagatggcg
//
LOCUS       DATMSK010000141         4567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_221773,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000141 DATMSK010000000
VERSION     DATMSK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221773"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VNZ84_04760"
     CDS             <1..344
                     /locus_tag="VNZ84_04760"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HWU66299.1"
                     /translation="LSKEDTEKAAPDYLMNDIVERVKGPGVQYKLLLQLADKGDDVND
                     ATAVWPDSRKTVELGTLTLNKAVADSKSAEKPLMFNPLQLTDGIAPSKDPILLARPTA
                     YAVSFGRRLAH"
     gene            complement(412..858)
                     /gene="msrB"
                     /locus_tag="VNZ84_04765"
     CDS             complement(412..858)
                     /gene="msrB"
                     /locus_tag="VNZ84_04765"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWU66300.1"
                     /translation="MLKWKDILKLAREGNLEPDHKVQKSIRAWREILDDEVFAVTRLS
                     ATERPFSSEMCAAFTPGRYACTCCTQVLFDSGQKFDSSSGWPSFDQPLAANAISYFSD
                     ESHGMQRIEVKCNVCDAHLGHVFPDGKTDSGLRYCINGKAIEFLAH"
     gene            1014..1937
                     /locus_tag="VNZ84_04770"
     CDS             1014..1937
                     /locus_tag="VNZ84_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HWU66301.1"
                     /translation="MIVRPRPHLLELFFIIKGSIVLRIMPLLVFVATLSAAVVSAYTF
                     RPDWLPRFDGGPFALLGITLSIFLAFRNNACYERWWEARKIWGEFVLTCRSLGRLSVI
                     LDEPSGGVSITRQQLLKFSIAYAHALLVQLRPDSSRQVLQAMLPTDLLQAVHTSRNPP
                     EAILRAMQQLLVQGLQDKKIDVIQFQMLDELVQKLAQAQASCERIQNTPIPFGYTLLL
                     HRTAYTFCFLLPFCFANTLGWATPFVTALAAYTLFGLDALGNELEEPFSLGANALPIN
                     ALTDVIEIDLREALGEKDIPLLPAPKDFVLM"
     gene            complement(1943..2197)
                     /locus_tag="VNZ84_04775"
     CDS             complement(1943..2197)
                     /locus_tag="VNZ84_04775"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HWU66302.1"
                     /translation="MGEGLADHRPDFSATGREWRTPPLWGIGLAKQVHPEAGFLHDGR
                     ARTLQEAILWHGGEAETAKEYFRHLNKQDRTALLQFLESL"
     gene            complement(2272..3276)
                     /locus_tag="VNZ84_04780"
                     /pseudo
     CDS             complement(2272..3276)
                     /locus_tag="VNZ84_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
     gene            complement(3285..>4567)
                     /locus_tag="VNZ84_04785"
     CDS             complement(3285..>4567)
                     /locus_tag="VNZ84_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU66303.1"
                     /translation="HALVSVFNKLLLGSGLLLVSLTTTAAEKVVVMTSYPQEMISQFE
                     AAFESQFPQYRLEVLWKQSNDALTYLTQHPGEVDAYWTPSRQNFLQLGKQGLLQKLDP
                     AWFKSPASLHGTLLNDPRAYYAATEIAGLGMVVAPTAFSKPKLSLPNDWLDLTQPMLS
                     GQVAFPLPTKIGFASGLIDAMLREKPWTEGWAMLGKITLNAHFIENGSTFITDEVSAG
                     RAMVGITMDFFAASAIAKGAPLQYIYPPVVAYSPAHVAIMQNAPHQQAAQAFAKFTLS
                     PAGQALLFAPDIRKLPVDPAAYAQRPAGDFNPYAAPTKAGEKVRDVTEQNMLNAYFEA
                     TLVKHQALLQSLFVNMNFARHLKRDTAKIDSLEQDLLQPVLSEQQLFSAENYTQFQSD
                     SAPVKEALIKHWSDLAKAHYQSVSNTLQTLLVAP"
BASE COUNT         1127 a         1111 c         1150 g         1179 t
ORIGIN      
        1 atttaagcaa ggaagacact gaaaaggcag cgccggatta cctcatgaat gatattgttg
       61 agcgcgtgaa agggcccggc gtacagtaca agttactgct gcaattggca gacaaaggag
      121 atgatgtgaa cgatgctacc gcagtttggc cagatagccg caagacggtg gaactgggca
      181 ccttgacctt gaataaagcg gtcgcagata gtaagtctgc agaaaaacca ctcatgttta
      241 acccgctaca gttaacggat gggattgccc cgagcaaaga tcctatcttg ctagcaagac
      301 caaccgctta tgcagtgagt tttggcagaa gactggcgca ctaaccagtc agaacgtctc
      361 atctgaacat tcagcgcggc gctgtcacca caggccgcgc ttttttgtgt tttagtgagc
      421 cagaaactca atggccttgc cattaatgca atagcgcaat ccgctgtctg tcttgccatc
      481 gggaaacaca tggcccagat gtgcatcaca tacattgcat ttcacctcta tgcgctgcat
      541 gccatggctt tcatcagaaa agtaggaaat ggcattggct gccagaggtt ggtcaaaaga
      601 aggccagccg gaagaagagt caaatttttg ccctgagtca aataatacct gggtgcagca
      661 ggtgcaggca tagcgtcctg gtgtaaatgc agcacacatt tcagaggaaa acggccgttc
      721 ggtcgcactc aggcgagtca cagcaaacac ttcatcatcc aggatttcgc gccaggcgcg
      781 tatggatttc tgtactttgt gatccggttc cagattgccc tctctggcca gtttgagaat
      841 atctttccat ttcaacatgc tgtttaccta taagtgtgaa atatcacgac aaatattgtt
      901 taaccaacgt tttcccatgt ctttttcaca catgcgcatc aaacgctggc agatagacta
      961 caaactatcg agtatatcat tatcgcaatg gttcgccagg caggagaatt cccttgatcg
     1021 tcagacccag accccatctg ttggagctat tttttatcat taaaggctcc atcgtgctca
     1081 ggatcatgcc attgttggtg tttgtggcca ccttgtctgc agccgtggtt tcggcctata
     1141 ccttcaggcc agactggtta cccaggtttg atggtggccc atttgcattg ttgggcatta
     1201 ccttgtctat ctttctcgct ttccgcaaca atgcctgcta cgaacgctgg tgggaggcca
     1261 gaaaaatctg gggtgagttt gtactcactt gcagaagcct tggccggttg agtgtcatac
     1321 ttgatgaacc gagtggtgga gtcagtatca cacggcagca gctccttaaa ttcagcatcg
     1381 cttacgcaca tgctttactg gtgcaattgc gccccgacag ctcacgccag gtattgcagg
     1441 ccatgttgcc gactgatctt ttgcaggcag tgcataccag ccgcaatcca ccagaggcga
     1501 ttttgcgcgc catgcagcaa ttgctggtgc agggattaca ggataaaaaa atagacgtga
     1561 tccagtttca aatgctcgat gaattggtgc aaaaactggc gcaagcgcag gcatcttgcg
     1621 aacgtatcca gaacaccccc attccatttg gctacacgct gttattacac cggacagcct
     1681 ataccttttg ctttttattg ccattctgct ttgccaatac gctgggctgg gcgacgccgt
     1741 tcgttaccgc cctggctgcc tatactcttt ttggcctgga tgccttgggt aacgaactgg
     1801 aagagccttt ttcactaggt gctaatgcct tgccgatcaa tgcgctgacg gatgtaattg
     1861 aaatagacct cagagaagcg ctcggtgaaa aagatatccc gctactgcct gcacctaaag
     1921 actttgtgtt gatgtaggtc ttttataggg actcaagaaa ttgcaataat gctgtgcggt
     1981 cttgcttgtt caagtggcga aaatattcct ttgcggtttc cgcttcgcca ccatgccata
     2041 agatggcttc ctgcaatgtc ctggcacggc catcatgcaa gaaccccgct tctggatgga
     2101 cttgtttggc caagccaatg ccccataacg gaggtgtgcg ccattcgcgg cctgtcgcgc
     2161 tgaaatcagg cctatgatct gccagccctt cgcccatgtc atgcaataat aagtcgtata
     2221 aggttgaatc gtgcgttgat ttaataatgc caagctggct tgctggccag tgcgcaattg
     2281 atcagtgtga cattcaatac attgtgcgcg tttgaataag gcaccaccac gttgtaatgc
     2341 ctgtgttgac gcggctggct gtggcacaga taacgccgat aaatagaatt gaatctcatc
     2401 cagctgttgg ttggtgagtt ctggcttttt gagttcaggt attgccaagc aggcaggctg
     2461 cgctagtgta cagtttgccg ttgcaaacag gctggacgta atgcccaaat cgccgtgaaa
     2521 agcattggca atttgctggc gtaaattggg cacattcgct ttccagccaa atttcccgat
     2581 caccattttt tgctgcgcaa tatcccaaac cttattcggc ctgccagcaa tcccttttgg
     2641 tttgcggcgc tgagcctgtc gcaaaatttc ttcatctgga atattgtcca ataaacccag
     2701 gccaaaaact gcaggcgcaa tccgtgccga agtcagcaca ttttccatgg ggccgtaggc
     2761 caattcagtg aaacgcaatt gcggctggcg caaaatgact tcttcgccat ctgccagtgt
     2821 taccggcatg ttctggtagt caatttgggc gatgccttcg cctgctacgc caggaatgcc
     2881 aaattcattc agttggtcac cataaatagg gtgtggaacc gcaccttgct gctgatctgg
     2941 tacgctaagc ctgaccagca tagttttcat tgtttgttct ggaccattgg gcgcaaagcc
     3001 acgaccgttg ccaggatggc aggcaacgca ggaaaaacgg ttatacaacg ggcccaagcc
     3061 agtgaaacgc tggtcttcac cgtcaatcgt ccaaacctgg cggaacaatg tgcggccttc
     3121 ctggaaggtt ttgagagaag ccggatcaag caagtttgaa tatggccgtg tgtaagcctc
     3181 tgcggtacga ggctcagtgg tcagcaatgc ctgttggtcg gcgattgcga cacatgccca
     3241 aggcagcatg gcaaaccaga gtagaccggt acgcatatct tacgtcaggg cgccacaagt
     3301 aaggtctgta gcgtgttaga gacagactgg tagtgggctt ttgccaagtc tgaccaatgc
     3361 ttgatcaagg cctcttttac aggcgccgaa tctgactgga actgggtata attttcagca
     3421 gaaaataatt gttgttctga taaaacgggt tgcagtaaat cttgttcaag tgagtcaatt
     3481 tttgcggtat cacgttttaa gtgccgggca aaattcatat tcacgaatag cgattgcagt
     3541 aaggcctgat gcttgaccaa tgttgcctca aaataagcat tcagcatgtt ctgttcagtc
     3601 acgtcacgca ctttttcacc cgcttttgtt ggtgccgcat agggattgaa gtcaccggcc
     3661 ggacgttggg cataagctgc cgggtcgaca ggcagtttgc ggatatcggg cgcaaataat
     3721 aatgcttggc cagccggaga tagcgtaaat ttagcaaatg cctgcgcggc ttgctgatgt
     3781 ggagcgtttt gcataatggc cacatgcgct ggcgaatagg ccacgacagg cggatagata
     3841 tattgcaatg gcgcgccttt cgcaatggct gaggcagcaa aaaagtccat ggtaatgcct
     3901 accatcgctc gtccagcaga tacttcatct gtaataaacg tcgagccgtt ttcaataaag
     3961 tgcgcattga gcgttatttt gcctagcatt gcccaacctt cagtccatgg tttttcacgc
     4021 agcatggcat caatcaatcc actagcgaag ccaattttag tcggcaaagg gaatgcaacc
     4081 tgtccagaaa gcattggctg agtaaggtcc agccagtcat ttggtaacga aagtttgggc
     4141 tttgaaaacg ctgtcggcgc taccaccatg cccaagccgg caatctcggt tgctgcatag
     4201 taagctcgcg gatcattaag cagcgttcca tgtaagctgg cgggcgattt aaaccatgcc
     4261 gggtctagtt tctgcaacaa accttgcttg cctagctgaa ggaaattttg ccgggaaggt
     4321 gtccagtaag catcaacttc gccgggatgt tgagtcaaat aggttaaagc gtcatttgac
     4381 tgtttccaca atacctccag acgatattgt ggaaactgac tttcaaaggc agcttcaaac
     4441 tgtgaaatca tttcctgcgg gtagctggtc atgactacca ccttttcggc agcggttgta
     4501 gtcaggctga ccagcaaaag cccactaccg agaagtaatt tgttgaatac gcttacaagg
     4561 gcgtggg
//
LOCUS       DATMSK010000142         7724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_362639,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000142 DATMSK010000000
VERSION     DATMSK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362639"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VNZ84_04790"
     CDS             complement(<1..267)
                     /locus_tag="VNZ84_04790"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HWU66304.1"
                     /translation="MALIKTQDFIESVADALQYISYYHPEDYIQALAAAYEKEASPAA
                     KDAIAQILTNSRMCAEGKRPLCQDTGIVVAFVKVGMQARFEGDMT"
     gene            complement(293..1942)
                     /locus_tag="VNZ84_04795"
     CDS             complement(293..1942)
                     /locus_tag="VNZ84_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWU66305.1"
                     /translation="MTQLAPYPQWQWEIPEFFNIGVACSDRHLGTVKADEIAMIVEDD
                     TFGTSTITFAELAARTDKFAQVLRDLGVQPGDRVLIRLPNSLDYPTAFLGTMKTGAIS
                     VPTSTLLTVEEVAYLAKDSGAVVLVTDAAAWATMSAKMQQNISQTPNLKHIVLTQLQG
                     AVEDSTVFNVLSLETALASVHQVTPAYATRGEDAAYLVYTSGTTGYPKGVLHAHRALL
                     GRQPAAQYWFNYSDSMQDRIMHSGKFNWTYVLGTGLMDPLYLGKTVIVHEGKNDAQKW
                     LELIAKHSATIFIGVPTIYRQLLQKTTATQADIPSVRHFMSAGEHLSDEVLTQWRERF
                     GLDIYEAVGMSEFSYYLSQSIFRPIRAGSAGFPQPGHAIVLLNPETLEPVAEGEEGMI
                     CVPDNDPGLFLSYWNLPEETAKYKHDGHFFTGDYAKYDADGYLWFLGRKDDIIKSFGY
                     RVSPYEIERVYKGHPAVADCAAVGEEIEKDKLLVVIYVIPKPDTEIAPEELMSFGKQH
                     LAAYKAPKTVYLAKEFPRTKNGKILRKDVSPAIAYQKSNAR"
     gene            complement(2168..3298)
                     /locus_tag="VNZ84_04800"
     CDS             complement(2168..3298)
                     /locus_tag="VNZ84_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWU66306.1"
                     /translation="MSQHMGEEPIALDARALGDYWPGIQLYYPPVKYAPSLGIYEDME
                     QAANRMVKHAHFTNAHTLIFDLEDGCRQKEMSRTLLRQELPKLRKVNERVTIAIRANS
                     FRTDEYEEDMKLVRDMGEYIDVVMLAKAGESYGAAEVRDLSSFLLSINPQITIQPIIE
                     HPKSLKIAPDLMQYDTVKHVVFGIHDFSKALGIHITPEHWIEELFYFINQLIFEARIA
                     GKGVIGGVETLIGSNIMPDKFVEPHDVRRWLDLHGDNESRVVYKHACQEAAMGLTGKQ
                     VIHPGHIHLCKTAYTPSPTEINQKVAILKAAIEADALLGGAIKFEGEMLDPPMFGKAL
                     QTLLRAHALRALKVSDMEFALHVLQLMPAQVVRQNWPYGILL"
     gene            complement(3295..3582)
                     /locus_tag="VNZ84_04805"
     CDS             complement(3295..3582)
                     /locus_tag="VNZ84_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HWU66307.1"
                     /translation="MLNAEILREAEQRRLAWRCRRGMLELDIVLQRFVNEHFSGLTLA
                     EMEQLDALLELPDNVFWDLIQGVNPQPGMAQSAAILEKLRASRPTAEQETA"
     gene            complement(3592..4353)
                     /locus_tag="VNZ84_04810"
     CDS             complement(3592..4353)
                     /locus_tag="VNZ84_04810"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HWU66308.1"
                     /translation="MRFSIYRFNPDVDKKPYMQDYDVQLEDSDQMLLDALMRIKELDE
                     SLSMRKSCREGVCGSDAMNINGKNGLACITKLSDLEEPVVLRPMPGLPVIRDLVVDMT
                     QFFDNYHSVMPYLQNHDAPPEKERLQSPEERAKLDGLYECILCGACTTSCPSFWWNPD
                     KFVGPAGLMQAYRFIADSRDQEQDARLENLEDPYRLYRCHNIMNCTDVCPKKLNPNAA
                     IAAIKDLKIEKAKAHKESKSGKNGPDKKIFGIKVL"
     gene            complement(4355..6142)
                     /gene="sdhA"
                     /locus_tag="VNZ84_04815"
     CDS             complement(4355..6142)
                     /gene="sdhA"
                     /locus_tag="VNZ84_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWU66309.1"
                     /translation="MADSQSIGATNIQHHQVDAVIVGGGGAGLRAALQLAESGADVAV
                     LSKVFPTRSHTVAAQGGIAAALGNVSDDSWLWHMYDTIKGSDYLGDQDAIEFMCRNAA
                     KAIIELEHFGMPFDRLENGRIFQRPFGGMTRNFGETPISRTCAVADRTGHAMLHTLYQ
                     RNVKANTQFLIEWFALDLIRDADGDVLGVIALEIETGDIHVIHAKATMLATGGGGRIF
                     QASTNAYINTGDGLGMAARAGLPLQDMEFWQFHPTGVLNAGVLITEGVRGEGGYLINS
                     DGERFMERYAPSAKDLASRDVVSRAMATEIKEGRGIGKHKDAVYLKLDHLGEDIISKR
                     LPGIREIAKTFAGVDPTNDPIPVVPTAHYMMGGIPTNLDGQVVVHDGQSERVVNGLYA
                     VGECACVSVHGANRLGSNSLLDLVVFGKAAGDKMAADIAANKTHKPLPANAADATLAR
                     VSRLESDSDGEKVAEVGLAMRQTMQKHCGVFRFPELLAEGVDAIQQVAQRAQYLKITD
                     KSKVFNTARVEALELDNLMQVALATMQAAHARQESRGAHAREDYPERDDANWLSHSLY
                     FANEQKLAYKPVRLKPLSVEPFELKKRVY"
     gene            complement(6153..6506)
                     /gene="sdhD"
                     /locus_tag="VNZ84_04820"
     CDS             complement(6153..6506)
                     /gene="sdhD"
                     /locus_tag="VNZ84_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HWU66310.1"
                     /translation="MLFELLTAKYPGMRRWLTQRLCALSMAAVFVLLWLRMVWLSPQG
                     YSEWQALFAPLWWRLLMLWFFACMLLHAWLGVRDVLRDYVPHLGIRAVLQWLVTITVW
                     GNALAVVWILFGMNF"
     gene            complement(6496..6906)
                     /gene="sdhC"
                     /locus_tag="VNZ84_04825"
     CDS             complement(6496..6906)
                     /gene="sdhC"
                     /locus_tag="VNZ84_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWU66311.1"
                     /translation="MIVKKQKKQRPKNLDLFTIRLPVNAVVSIMHRASGVVLFLIQPV
                     LLWALYLSLSSEQDYGAVTATLHQWPFKLLLTVLAAAFFHHFYAGIRHLAMDVHWMTT
                     LQKARFSSRVVLWLVAVTMLFVSWAIWLRGWHVV"
     gene            complement(6918..>7724)
                     /locus_tag="VNZ84_04830"
     CDS             complement(6918..>7724)
                     /locus_tag="VNZ84_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HWU66312.1"
                     /translation="NLEDIAAPHCFYIENELKKRLDIPVFHDDQHGTAVIVAAALLNA
                     LDLQGKTLPRAKIVFLGAGAAGCSCAKLLKLMGATHITMVDRKGVLDSNRDDLHDNNR
                     ELAITTGSVKTLADAMNDADVFIGVSAKNGLPPELVATMAAKPVIFALANPDPEILPD
                     QVFAVRDDVIMATGRSDFPNQVNNALCFPYLFRGALDAQAKQITEQMKVAAAKALSQL
                     AREPVPQQVLDAYQLQSLSFGKEYIIPKPFDPRLMDYVAGAVARAANASE"
BASE COUNT         1891 a         2142 c         1837 g         1854 t
ORIGIN      
        1 ggtcatgtca ccctcaaaac gcgcctgcat gccgacttta acaaacgcca ccacaatgcc
       61 ggtatcctgg cacagcgggc gtttaccctc ggcacacata cgcgagttgg tcaaaatctg
      121 tgcaatcgca tctttggcgg caggcgaagc ctctttttca tacgccgcag ccagtgcctg
      181 gatataatct tccgggtgat agtacgaaat gtattgcaag gcatcggcaa cactttcgat
      241 aaagtcttgg gttttaatca gggccatgtt ctgtgtcttt caggcgggca tctcaacggg
      301 cattgctttt ctgataggca atggcaggac tcacatcttt acgcaagatt ttaccatttt
      361 tggtccgtgg aaactccttg gccaggtaaa ccgttttagg cgccttgtaa gccgcaaggt
      421 gctgtttacc aaacgacatc agttcttcgg gcgcaatttc agtatccggt ttggggatca
      481 cataaatcac caccagcagc ttgtcttttt caatttcttc gcccactgca gcgcagtccg
      541 ccacagccgg atgacctttg tacacacgtt caatctcgta tggagataca cggtaaccaa
      601 agctcttgat aatgtcatcc ttgcggccga ggaaccaaag atagccatcg gcatcgtact
      661 tggcatagtc accggtaaag aagtgaccat cgtgcttgta tttggcagtc tcctccggca
      721 ggttccaata gctcaggaat aaacctggat cattatcagg cacacaaatc atgccctcct
      781 cgccttcggc caccggttca agcgtttctg gatttagcaa cacaatggca tggcctggct
      841 gtggaaatcc cgccgacccc gcacggatcg gccggaaaat gctttgtgac aggtagtaag
      901 aaaactcact catccccact gcttcataaa tatccaaccc gaagcgctcg cgccattggg
      961 tcagcacttc atcagacaaa tgttctcccg cgctcataaa atgacgcacg ctgggaatat
     1021 cggcctgggt agccgtcgtt ttttgcaaca actggcggta aatcgttggt acaccaataa
     1081 aaatggtggc gctatgtttg gcaatcagct ctagccattt ttgtgcatcg tttttgccct
     1141 catgcacaat cacggtttta cccaagtaaa gcggatccat gaggccagtg cccaagacat
     1201 aggtccagtt aaacttgcca gaatgcatga tacggtcttg catgctgtca ctataattaa
     1261 accagtattg tgcagcaggc tggcggccca gcaaggcacg atgcgcatgc aacacgcctt
     1321 tgggatagcc cgtggtgccc gaggtataca ccaggtaggc tgcatcttca ccacgtgtgg
     1381 catacgctgg cgtaacctga tgcacacttg ccaaggctgt ttctagcgac aatacattga
     1441 ataccgtcga atcttcaaca gcaccctgca attgggtcaa aacaatgtgc tttagattag
     1501 gcgtttgtga gatattctgc tgcattttgg cagacatggt tgcccaggca gcagcatccg
     1561 tcaccagtac gactgcccca gaatctttgg ccagataggc cacttcttca acggttaaca
     1621 aagtagaagt tggcactgaa atcgccccgg ttttcatcgt gcctagaaaa gcagtcggat
     1681 aatcgagact gttaggcaaa cgaatcagga cacggtcacc cggctgaacg cccagatcac
     1741 gcaacacctg ggcaaacttg tcggttctgg cagcgagctc ggcaaaggtg atagtactag
     1801 tgccaaacgt gtcgtcttcc acaatcatgg caatttcatc cgccttgacg gtgcctaaat
     1861 ggcggtcact gcacgccacc ccgatattga aaaactcagg gatttcccat tgccattgtg
     1921 gataaggggc taactgtgtc atgttcaaac ctttccaatc actctatgca cgcatggttg
     1981 atgcgatgct gttatatgca tttcttttgg aaatgccttt taaatcttca atgtcgctta
     2041 tacgctcatc aatgagcgag cgggatgaag cacaaggcgc ccggaacaca gaaaccggaa
     2101 tgtacttaaa gtacatgagg atttcgagta ccgcgcaacg ccgtgattcg cccgcgcagc
     2161 cattgattta aagcaagatg ccgtagggcc agttttgtcg caccacctgc gccggcatca
     2221 actgcaatac atgcaatgca aactccatgt cactcacttt gagggcacgt aaagcatgcg
     2281 ccctcaatag cgtttgcaag gccttaccaa acattggtgg gtccagcatt tcaccttcaa
     2341 acttgatcgc cccacccagt aaagcatcag cttcaatcgc cgccttcagg atcgctactt
     2401 tctggttgat ctccgtcggt gatggcgtgt aagcggtttt acataggtgg atatgcccag
     2461 gatgaataac ttgtttaccg gtcaggccca ttgcggcctc ttggcaggcg tgtttgtaca
     2521 ccacccgcga ctcgttatcc ccatgcaaat ccagccagcg gcggacatca tgtggttcga
     2581 caaatttatc cggcatgata ttgctaccga ttagcgtttc cacaccaccg atcacgcctt
     2641 taccggcaat cctggcttca aaaatcaact ggttaataaa gtaaaacagc tcttcaatcc
     2701 agtgctcggg cgtaatatgg atgcccaacg ctttggagaa atcgtggata ccgaatacga
     2761 cgtgtttcac ggtgtcgtat tgcatcaaat ccggggcaat tttgagcgat ttcgggtgtt
     2821 cgataatcgg ctgtatcgtg atctgcgggt taatactgag caaaaagctc gacagatcac
     2881 gtacctcagc cgcaccataa gactccccgg ctttagccag catgacgacg tcgatatact
     2941 cgcccatgtc acgcaccagt ttcatgtctt cttcgtactc atcggtacgg aaagagttgg
     3001 cccggatggc gatagtgacg cgctcattca ccttgcgcag tttgggtagc tcctgccgca
     3061 gcaaggtacg gctcatttcc ttctgacggc aaccatcttc caggtcgaaa atcagcgtat
     3121 gtgcattggt aaaatgtgca tgcttgacca tacggttggc cgcctgctcc atgtcttcat
     3181 agatacccag actgggcgca tatttcaccg gtgggtaata caactgtatc cccggccagt
     3241 aatcgcctaa tgccctggca tccagtgcaa ttggctcttc tcccatgtgc tgactcatgc
     3301 ggtttcctgt tctgccgtgg gccggcttgc tcttaatttt tcaaggatgg cggcagactg
     3361 ggccatgcct ggttgcgggt tgacgccctg gatcaagtcc cagaacacgt tgtccggcag
     3421 ttccagcaag gcatccagct gttccatttc ggccagtgtt aggccactga agtgttcgtt
     3481 cacaaagcgt tgcaatacaa tgtccaactc cagcatgcct cgacggcagc gccaagccaa
     3541 ccgccgctgt tctgcttcac gtaaaatttc agcattcaac atcgtccgct cctataacac
     3601 cttgatgcca aagattttct tgtcaggccc atttttgcca gacttggatt ccttatgcgc
     3661 cttggctttt tcaatcttga ggtctttaat cgccgcaata gccgcgttcg gattcagttt
     3721 tttcgggcac acatcggtac agttcatgat gttgtggcaa cgatacaaac ggtaagggtc
     3781 ttccaggttt tccagtcgcg catcctgttc ctgatcgcgg ctatcggcaa taaagcggta
     3841 ggcctgcatc aagccggcag ggccgacaaa tttgtccggg ttccaccaga aactcgggca
     3901 ggaagtggta caggcgccgc acaaaataca ttcatacaaa ccgtcgagct tggcgcgctc
     3961 ttcagggctt tgcaggcgtt ctttttccgg tggcgcatca tgattttgca gatatggcat
     4021 gaccgaatgg tagttatcaa agaactgtgt catgtcgacc accaggtcac gtatcacagg
     4081 caggcctggc atagggcgta acaccacagg ttcctcaagg tcactcagct tggtaatgca
     4141 agcaaggcca tttttaccat tgatattcat ggcatcgcta ccacagacac cttcacggca
     4201 ggatttacgc atgctgagtg actcatccag ctccttaatg cgcatcaagg catccagtag
     4261 catctggtcg ctgtcttcca gctggacatc gtagtcctgc atatagggct ttttgtctac
     4321 gtcgggattg aaccggtaaa tagagaaacg catatcaata cacccttttc ttcaattcaa
     4381 atggctctac ggataatggt ttgagacgca ctggtttgta agccagtttt tgctcattgg
     4441 cgaaatacag tgaatgactc agccagttgg catcgtcccg ttcaggataa tcttcacggg
     4501 cgtgcgcacc acgactttcc tggcgggcat gtgcggcctg catggtcgcc aacgcaactt
     4561 gcatcaggtt atccagttcc agtgcttcga cccgggcagt gttaaatact ttgcttttgt
     4621 cggtgatttt taagtactgt gcacgctgtg caacttgctg aatggcatca accccctctg
     4681 ccagtaattc ggggaagcgg aacacaccgc aatgtttttg catggtttgg cgcatcgcca
     4741 gaccaacttc agccaccttc tcgccatcac tatcactctc cagtcgactc acgcgtgcca
     4801 gcgtggcatc tgcagcattg gccggtaatg gtttatgcgt tttgtttgcg gcgatatcag
     4861 cagccatctt gtcaccagcc gccttgccaa atacgaccag atccagcaat gagttagagc
     4921 ccagacgatt ggctccatgt accgaaacac acgcgcactc gccgactgca tacaatccat
     4981 taacgacgcg ttcggactgg ccatcatgga cgacgacctg gccatcgagg ttggtcggaa
     5041 taccgcccat catgtaatgc gccgttggta cgacggggat aggatcattg gtgggatcca
     5101 cgcccgcaaa tgttttggcg atttcacgga tgcctggtaa acgtttgctg ataatgtctt
     5161 cccccaaatg atccagtttc aggtacaccg catctttgtg tttaccaatg ccgcggcctt
     5221 ccttgatttc ggtggccatc gcacgcgata ccacatcacg gctggcgagg tctttggcac
     5281 tgggggcata gcgctccata aagcgctcac catcactgtt gatcaggtaa ccaccctcgc
     5341 cgcgcacgcc ttcggtaatc agcacaccgg cattgagtac cccggtcgga tgaaactgcc
     5401 agaactccat atcctgtagc ggcaaccctg ctcttgcggc catgcccaga ccatcgccag
     5461 tattaatata ggcattggta ctggcctgaa aaatacggcc accaccgcca gtcgccagca
     5521 tggtggcttt ggcgtgtatc acgtggatat cgccggtttc aatttcgagc gcaatcacac
     5581 ccaaaacatc gccatcagca tcacgaatca aatcgagtgc gaaccattca atcagaaact
     5641 gggtattggc tttgacgtta cgctgataca gcgtatgcaa catggcgtgg ccggtacgat
     5701 cggccacggc acaggtacgt gaaatagggg tttcaccaaa attgcgggtc atcccaccaa
     5761 atgggcgctg aaaaatacgg ccattttcca agcggtcaaa cggcatgcca aaatgttcca
     5821 gctcaataat cgccttggcg gcattgcggc acataaactc aatcgcatcc tggtcaccca
     5881 ggtagtctga acctttgatg gtgtcataca tgtgccataa ccagctgtca tccgagacat
     5941 tccccagcgc cgcggcaatc ccgccttgtg ctgccacagt atgtgagcgg gttgggaata
     6001 ctttggaaag cacagccaca tcagcgccag actcagccag ttgcagggcc gcgcgcaagc
     6061 cggcgccacc accgccgacg atgacggcat cgacctgatg atgctgtata ttggttgcac
     6121 ctatcgattg agaatcagcc atgccactca ccttaaaaat tcattccaaa taaaatccag
     6181 acaactgcca gtgcattacc ccatacggtg atagttacca gccattgcaa caccgccctg
     6241 atgcccaagt gtggtacata atcacgtaac acatctcgca cgcctaacca ggcatgtaac
     6301 agcatgcaag caaaaaacca cagcatcagt agccgccacc ataggggtgc aaatagcgcc
     6361 tgccactcgc tgtagccttg tggcgaaagc caaaccatcc ttagccatag cagcacaaaa
     6421 acggcggcca tggacaaggc acataatctc tgcgtcagcc agcgacgcat gccaggatat
     6481 ttggctgtca gcaactcaaa caacatgcca gcccctcagc caaatcgccc aactcacaaa
     6541 caacatggtg accgctacca gccaaagtac aacccggctg gagaagcgcg ccttctgcaa
     6601 ggttgtcatc cagtgcacat ccatcgccaa gtgtcgaatg cccgcataaa aatggtgaaa
     6661 aaatgcggca gccaacaccg tcagtagcaa tttgaacggc cactgatgaa gcgttgccgt
     6721 gaccgcacca tagtcttgct cactagatag cgacaaatac aatgcccaaa gcagcacagg
     6781 ctgaatcagg aataacacca ccccactggc gcgatgcata attgacacca ccgcattcac
     6841 cggcaggcga atcgtgaata aatccagatt cttggggcgc tgtttctttt gctttttgac
     6901 tatcatgcta aatacgctta ctcgcttgca ttggccgcac gcgccactgc gccagccaca
     6961 taatccatta aacgcgggtc aaatggtttg ggaataatgt actctttacc aaaggataag
     7021 gactgcagtt gataggcatc gagcacttgt tgcggcactg gctcgcgtgc tagctgactg
     7081 agcgctttgg ccgctgccac tttcatctgc tcggtaatct gtttcgcctg ggcatccagt
     7141 gcgccgcgga acagataagg gaagcacaag gcattattca cctggttagg aaaatcgcta
     7201 cggccagtcg ccataatcac gtcatcgcgc acagcaaaca cctggtcagg caaaatttcc
     7261 ggatcaggat tggctaaggc aaaaatgaca ggcttggccg ccatcgttgc caccagttcc
     7321 ggcggcaaac catttttggc agacacccca ataaacacat cggcatcatt catggcatca
     7381 gccagcgttt tgacactgcc tgtagtgata gccaattcac ggttgttatc atgcaaatca
     7441 tcacggttac tatccagcac acctttacgg tcaaccatgg tgatatgggt cgcccccatc
     7501 aatttgagca acttggcaca tgagcaacct gccgcacccg cgcctaaaaa tacaatcttg
     7561 gcgcggggca acgttttgcc ttggagatca agtgcgttca acaacgcggc tgccacaatg
     7621 accgctgtgc catgctggtc gtcatgaaat accgggatat ccagacgttt tttcagttca
     7681 ttttcaatat agaaacagtg cggcgcggcg atgtcttcga gatt
//
LOCUS       DATMSK010000143        21991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_269372,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000143 DATMSK010000000
VERSION     DATMSK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 21991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21991
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269372"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..903)
                     /locus_tag="VNZ84_04835"
     CDS             complement(136..903)
                     /locus_tag="VNZ84_04835"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HWU66313.1"
                     /translation="MKLKGCVVALIFLQTWLTPVFAAACHAESGQYVIPLLELYTSEG
                     CSSCPPADRWLSSLQSRQGEVNALAFHVDYWDGLGWKDRFAQEAFSDKQRTQASQTGS
                     NVVYTPQFTLNGYDFRGWSDSRLDRLLQKINMQPAQLTLALQQLNEDNGQIAFRTTVT
                     TPQQHLPVQLWLAVYQHGLHTQVKAGENNGRVLNHDFVVLQHMGPYSLDQLNILRHLV
                     PDQSLLAHANGVVLYARHALNGEVLQSLQLPVCRPGH"
     gene            complement(999..1895)
                     /locus_tag="VNZ84_04840"
     CDS             complement(999..1895)
                     /locus_tag="VNZ84_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWU66314.1"
                     /translation="MRYQVEHQTIYSYDLPVIQSQQLLHMMPRQTPFQTCVSYTLACE
                     PIPAEQSQRLDFFGNHNHYLTMLATHQVLQVTSRFQVELQPRPPITALMQSPAWEQVQ
                     AMLQAQGAQHLEAVPFIYATNRVKSSEALAAFAKNCFTPQRSLLDAAMALTQKIHAEF
                     EFDAQATDVSTPLDEVLKGRRGVCQDFAHLMIGALRSLGLACRYVSGYILTHPPPGQP
                     RLVGADASHAWVSVYCPVYGWVDFDPTNNCLVQQEHITVAWGRDFGDVSPMRGVVLGG
                     GRQQLEVSVTVTPLEATTMLQV"
     gene            complement(1906..4446)
                     /locus_tag="VNZ84_04845"
     CDS             complement(1906..4446)
                     /locus_tag="VNZ84_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWU66315.1"
                     /translation="MQSVLNQLYFSDRYNELLDSSGQARPHWQALVSQLETEEPTAMR
                     KRIEAVQRQIRDNGVTYNVYADTHGVQRPWDLDVLPLIIPQEEWQHIAASVAQRATLL
                     NRVLLDIYGEQRLLSSGQLPPALIYGHSGFLPSCVGIPHYDNMALHSYAVDLARAPNG
                     QWWVVSDRTQTPTGAGYALENRTIISSAFPELFRDLNIERLSGFFASMRDSLAHWGHR
                     VAANQAQTVPGVTALGQGEPPLIVILTPGPYNETYHEQSFLAGYLGYPLVHGSDLTVR
                     NGIVWLKTIAGLKPVHVILRRIDDDYCDPLELNADSLLGVAGLTQAARLGNVLIANAL
                     GSNVLQSGALLGFLPNLCQSLLGEALRMPSVATWWCGEPAALEYVIQHLDELVIKPAY
                     PQLRSSPIFGEDLNASQKESFIAKLRAQPGHYIAQEQVDISHAPVLTNHHQQPQLGTL
                     AVSLRVYAFATPNGYAILPGGLSRVASGKDARVVTMQRGGTSKDTWVLSQDSQPPFSL
                     LRKTNSSQDLVRENAYLSSRMAENLFWYGRYSVRNLQKAITLRATIRALVEYTPESRA
                     GEWPTMQGLCQWFELLPSPKDEEESANWQPWTDDDIEPLLVQAVFSQQSTSLATSVQQ
                     LFQQAFNLRERISNDHWRTVNVLSRLFITRHQHASLQDALSYIEQAINHFVTLTGFAM
                     DWMTRDLGWRFMSLGRRIERLQFMCVLLSRALNMPQHSNLEWLLQVTNNLVTYRARYA
                     AQPEWLPVLDLLLMDENNPNSVVFQINGLVKYLEEMDAQDNAGGIATELNGCWQTLLQ
                     LDPDQCFIAGNAALADWLNQTYNVTLNLSDRLSMRFFSYASPLVTPGY"
     gene            complement(4507..7797)
                     /locus_tag="VNZ84_04850"
     CDS             complement(4507..7797)
                     /locus_tag="VNZ84_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWU66316.1"
                     /translation="MAIRVALHHRTSYKFDRLVNLSPHEVRLKPAAHARTPVLSYSLT
                     VNPEKHFINWQQDPYGNYIARFVFPEMVRELDFTVDLVADMTVINPFDFFVEKYAEHF
                     PFNYTEQQAFELTAYLQAEPMGPLLEAWMAVVRAEVVKHDIGIVDFLVAVNQRLQGDI
                     GYTVRMEPGIQTPEDTLQKRTGSCRDSAWLLVQVMRNLGLAARFVSGYLIQLKADVEA
                     LDGPSGTDKDFTDLHAWTEVFIPGAGWVGLDPTSGLMAGEGHIPLACTALPSSAAPVT
                     GFTDVAEVTFSHEMKVTRIHEDPRVTKPYSEKDWQAILKLGEQVDKQLTKMDVRLTQG
                     GEPTFVSIDDMDGAQWNTAALGEHKRKLAGELALRLKDHFAPHGFLHYGQGKWYPGEP
                     LPRWAVNIYWRPDGKPVWRDASLFSAEGPGDGYGIKQAQVFATHLADMLGFPKACVMP
                     AYEDVMSLAQQEQQLPANIDPLKADLKDAAERKRLASLLSQGLGQVVGYIMPLKPDES
                     RSAGEWLTSKWPLRREHLYLLGGDSPMGLRLPLSSLPWQLPADIEPEFAPDTFAEKPA
                     LDEAKTPKVRVKHQTTQPTAREVIHTALCVQVRQGRLYVFMPPVTRLEDYLDLLSAIE
                     QTASDLKLKLWLEGYTPPRDSRLKLLSVTPDPGVIEVNIHPAKSWKELVANVGTLYEE
                     ARLCRLATEKFMLDGRHTGTGGGNHVTLGGETAADSPMLRRPDVLRSLITYWQNHPAL
                     SYLFSGTFIGPTSQAPRVDEARDDNLYELEIAFQQMEKMLSSGKESQQPWLVDRLLRN
                     LLVDLTGNTHRSEFSIDKLYSPDGPTGRLGLVEFRAFEMPPHARMSLLQSLLLRALVA
                     RFWKTPYTGKLVHWGTELHDRWMLPQFLAQDIADVVADLREAGYAFEQRWFDPFVEFR
                     FPRYGTVAYHGIELELRQAIEPWNVLGEEMSGGGTARYVDSSVERMQLRVRGLTDGRH
                     AVTCNGRQLPLQPTGNAGEFVAGIRFRAWSPWSALHPTIPVQAPLVFDLVDTWNAKSL
                     GGCTYHVSHPGGRNYSTFPVNANEAEARRFARFWSHGHSPGRVDVVKEPPNPRFPFTL
                     DLRWQAKL"
     gene            complement(7923..8765)
                     /locus_tag="VNZ84_04855"
     CDS             complement(7923..8765)
                     /locus_tag="VNZ84_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWU66317.1"
                     /translation="MEISIIILILAIIAIVKGVRIVPQGEEWVVERLGKFAGILTPGL
                     HVINPILSTISYKVTTKDIILDVPEQEVITRDNAVILANAVAFIKVTNIERAVYGIEN
                     FREAMRNMVQTSLRSIIGGMDLNHALTSRDRIKAELKEAIADEALDWGLTVKSVEIQD
                     IKPSANMQHAMEQQASAERERVAVVTRAEGQKQSLILNAEARLEAARKDAEAQMVGAK
                     ASAEAIKMIAEAVKENNSSATFLLGDRYIQSLQKMADSSNSKIVVMPGDVVSAVKNLV
                     GGSK"
     gene            complement(8843..9298)
                     /locus_tag="VNZ84_04860"
     CDS             complement(8843..9298)
                     /locus_tag="VNZ84_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWU66318.1"
                     /translation="MSTVQLVWSWGVIGLVLLCLEMMSGTLYLLWPGAAALVMALFSW
                     LFPAFSLAWQIVIYAIFSVLAVAIWRKREQAHPAPRVGQAQGEEIGRQGVITVAVSPA
                     QPGKIRFSQGVMGSKEWTVYADVEIAINQPAKIMAVEGNCLRVAPLTQH"
     gene            9432..9998
                     /locus_tag="VNZ84_04865"
     CDS             9432..9998
                     /locus_tag="VNZ84_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWU66319.1"
                     /translation="MEIVTLVIFAVLTLYIIMLYNSLVSVKNNISKAWSNIEILLKQR
                     HDELPKLIDTCKQYMKHEQETLERVMLARSQVSKAQQSHDVDALGTAEGALRSGLGHL
                     FAVAEAYPELKANENFLHLQSRISGLENAIADRREFYNESVNINNVRIEQFPDLLVAK
                     LFNFPRFELMRFASEELRDIDIGKRFNS"
     gene            10007..10918
                     /locus_tag="VNZ84_04870"
     CDS             10007..10918
                     /locus_tag="VNZ84_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66320.1"
                     /translation="MLQFLRGHDSDAITSVGHLTLLLMAIYAHNQRVWIGCCSLIGLI
                     SLWAWAANYQRCRVISDTPTSRIASAAQGYVELYGRAAMTYNNLLISPYSHTRCIWFR
                     YRVYEKEDKGWRQVTNHTSDSTIEITDGTGSCVIDPDHAEVISPDRRVVTQGSYKYVE
                     ELLRSDSVYALGEFSTIGGANSVLDLKSDVAALLATWKENKQTLLQRFDLDGNGEIDI
                     KEWELARRAAVREVEKEHRDIRAADGVHVMRAPKNGQPFLLSNLSPQKLRNIYRCWGA
                     VHLTILFLAVAGALWFWKGHTLTELFI"
     gene            11120..12376
                     /locus_tag="VNZ84_04875"
     CDS             11120..12376
                     /locus_tag="VNZ84_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HWU66321.1"
                     /translation="MTQPPTRLTLATDRYERLAQIRDCFPQATGPDLAETWQGGRQMA
                     LQRLSAIDAVAYGRSRNHLDGSVTRLSPYLRHGCLSLQEAINTIQQDFGAQGIKLIAE
                     LSYRDYFRQVWYRFGDAIQQPLEPSKVIPGENSLPEFIGQGFTGLVCMDEIARALQQE
                     GYVHNHARMWFAAYVLHWLKVDWRQAADWFEQHLLDGDLASNHLSWQWIASSFSHKPY
                     YFNKENIIKFGGQRWCQQCRVRCPFDASYEALEQQLFTTSSGAKTTLPNVVTRAAHPQ
                     QADDLSQAAKANTLVWLHDEMLSPEHPLLTTKGAVAFVFDPALYGQWPLKRLQFMADC
                     LSEIPQAEIWMGSTNEVLAGLSQEQIISQETPNTAIKAVVKDARLTWIKEPSLYNTSF
                     SDHELMRFSRYWKKVEPLVFAETHAG"
     gene            complement(12431..13942)
                     /locus_tag="VNZ84_04880"
     CDS             complement(12431..13942)
                     /locus_tag="VNZ84_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HWU66322.1"
                     /translation="MTFKLAAMTVAISLALPTLANATPSLITFGALSGTTDLSSFSGV
                     LENGVDHQNVLGGMGSGLAWAGGNTFLALPDRGPNATVYNPLVDNTTSYIARFQTLKL
                     DLTQTFNGGSYSYSLTPTLTGTTLLYSNTPLNYGSGSAGTGSVGGTSYTLGSGATVDA
                     GKYYFNGRSDNFLAGSNSSNPNNGRFDPEAIRVSNDGKIVYISDEYGPYVYQFDRATG
                     ARTNVYNLPSAFTASSLSSQGANEISGNSSGRVANKGMEGLAITPDGKALVGFEQSPL
                     IQDGGDGGRTNRIVTIDTVTGATKQFVYDNFLSDKNKSYNSSEILAVNDHTFLVLERD
                     GKGLGDDSKADVKRIYKIDLNDANGDGVSGDLAVDIGSLNAGAGISGQSNLLGYAVQK
                     SLFLDLKSALTDVGISNTNIPSKLEGMTFGEDIMEGGVLYHTLYVANDNDFLPATAGA
                     NKFFVFKFTDADLNGSAFVNQALSVSAVPEPGNVSLLVSGLLLMTWIQRRRQR"
     gene            14068..16965
                     /locus_tag="VNZ84_04885"
     CDS             14068..16965
                     /locus_tag="VNZ84_04885"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWU66323.1"
                     /translation="MTTSNQNSNPARELAKSFDPKSIESQWYAFWESKGFYAAGLNPD
                     VQDNFCILLPPPNVTGTLHMGHGFNQTIMDALTRYHRMRGDNTLWQPGTDHAGIATQI
                     VVERQLDAQGVSRHDLGREKFLKKVWEWKEYSGGTITKQMRRLGTSPDWGRERFTMDA
                     GLNKVVTETFVRLYNEGLIYRGKRLVNWDVKLGTAVSDLEVVQEEEDGSMWHINYPLA
                     DGSGQLTVATTRPETMLGDVAVMVNPEDERYAHLIGKRVSLPLCDREIPIIADEYVDK
                     EFGTGVVKVTPAHDFNDNMVGQRQGLDKIVILNLQGFVPETAEVYAADGSTKAPIALP
                     ANLVGLERFAARKQVVADLEAGGFLVKVEKHKLKVPRGDRTNVVIEPMLTDQWFVAMS
                     KAGEDGKSITEKALEVVANGEIKFYPENWVNTYNQWLNNIQDWCISRQLWWGHQIPAW
                     YSDDGKVYVAHDEAEAKAMAAKDGYTGALTRDNDVLDTWYSSALWPFSTLDWTGDEAI
                     DAQNQALQQFLPSSVLVTGFDIIFFWVARMVMMTKHITGKIPFKHVYVHGLIRDAEGQ
                     KMSKSKGNVLDPIDLIDGIDLESLVKKRTTGLMNPKDAEKIEKRTRKEFPEGIAAYGT
                     DALRFTFASLASPGRDIKFDLQRCEGYRNFCNKLWNATRFVLMNTEGQDNGFGVESCE
                     DGGRKFSQADRWIVSLLQRTEAEVERAFNDYRFDLAAKAIYEFVWDQYCDWYLEIAKV
                     QIQGGSEAEQRATRRTLLRVLETILRLAHPIIPFVTEEIWQTVAPMTDLKDKSRGTES
                     IMLQAYPKADLEKIDETAEAWVATLRTAVEACRSLRGEMSISPAAKVPLIAAGDAAQL
                     AAYAPYLKSLAKLENVDIVAELPEADAPVMLAGDFKLMLKVEIDVAAEKERLGKEIAR
                     LQGEIAKANGKLGNESFVARAPVAVVDQEKARLAEFSANLEKLQAQLAKLK"
     gene            complement(16995..18017)
                     /locus_tag="VNZ84_04890"
     CDS             complement(16995..18017)
                     /locus_tag="VNZ84_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase"
                     /protein_id="HWU66324.1"
                     /translation="MSKGVRLSEKWFNRALWMVAIIFAWFLTGLGKSIIGDLPRVETY
                     YTTEHFVNHAEIDPLRTELKTLAWQRQTLADQLEQVNLQLIIRQKDYQAARTTFDNWV
                     KTRDTTDEVDDLVSRTRALDALKKLERDAEREIEDIQQQQTNISQHETQLNTQVNSLE
                     DAARQQLDDAYEAQAMRVFLYRLLLTLPLLALAAWLFAKKRQSQYWPFVWGFIIFALM
                     AFFVELVPYLPDYGGYVRYTVGILLTVVGGKYAIAALQNYLEQQKLAESQPEFQRREE
                     LGYDTALARLAKNVCPGCERTVDLKNEQNDFCTHCGICLHNYCNACQSRKNAFAKFCH
                     KCGTVA"
     gene            18158..18370
                     /locus_tag="VNZ84_04895"
     CDS             18158..18370
                     /locus_tag="VNZ84_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWU66325.1"
                     /translation="MCEIDEENPKEVLCSCSGTTRGLIIELVKQGKDLEGISRYSGAL
                     SGCGGCEWEISELVNKLQAGDSTPKP"
     gene            complement(18367..19113)
                     /locus_tag="VNZ84_04900"
     CDS             complement(18367..19113)
                     /locus_tag="VNZ84_04900"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWU66326.1"
                     /translation="MQDNLLLRYSRHIMLPQIEYAGQEKLTQSHALIIGAGGLGAPVA
                     MYLAAAGVGTLTICDFDQVDLTNLQRQIIHTTSAVGQNKALSAQATIASLNPEIIVHV
                     ETTRLSEEALAGRVAATDVVIDCSDNFATRYLLNQLCFDHKTPLVSGAAIRFEGQLSV
                     FDFRHDESPCYHCLYPDVGDDQELRCADNGVFAPLVGMIGTAQAAEALKILLNIGQTM
                     KGKLLLLDALSAEWRTIRLKKDASCAVCGH"
     gene            complement(19129..19854)
                     /locus_tag="VNZ84_04905"
     CDS             complement(19129..19854)
                     /locus_tag="VNZ84_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWU66327.1"
                     /translation="MSTFCTQCRPAHKFIPGFSLLEMALALVILSLMMGWLMTPLRVQ
                     HGLQKYQQTEQRLVQAQQALLGHAMIYHYLPCPDTDQPPDGWENVLANQSCASDEGVL
                     PWAQLGLVASDAWGRFFRYRVDSTFTHHAQWFSISAAESASNLQVIGEAGAVTSTPSR
                     PAAIILSHGENGLGGIQSVSGGQTYVMAMPTHPDEQENADGDLVFVDKSQQQTGGAVF
                     DDRLIMLSPKVLIQQMVQAQRLP"
     gene            complement(19851..21005)
                     /locus_tag="VNZ84_04910"
     CDS             complement(19851..21005)
                     /locus_tag="VNZ84_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66328.1"
                     /translation="MAVWVVLLLLSGAGGWMLYTSSNTLSARHLMMQKTITSLALAQQ
                     ALLAYAQQPLGVTQCESNCPRPGDLPCPDRNNDGIAETSCSNTARLGRLPWKTLGIGD
                     VRDGSGERLWYAVSERYKNNPRILPLNLDTSGTWSMLSAEGVLWDATQGDGLVAVLIA
                     PMQPLMREDGWVQQREEASSEIAKHYLDIHGLDNANPVEKAVNGFVMAASSPHFNDVV
                     WPITTGSMHQQMQKQVLSELKRSLRCAVAPCTTHPSPAATEDDRCLGFQSMPAGQCLS
                     ATANIGRLPLDAAAHWPGATQHLLDGDARHHWFQQNGWREQVFYQAGPTQATLVVAGE
                     RLPGQVRENVADKGNLNAYLEASTLQILQVQNASALTLPSNDILDRVMQP"
     gene            complement(21032..21646)
                     /locus_tag="VNZ84_04915"
     CDS             complement(21032..21646)
                     /locus_tag="VNZ84_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66329.1"
                     /translation="MSDNSYWRTLRGVFIGWLAALGVLAVFVAIIMHRQSGAEKALAQ
                     AEQKWRHSQQQSAQYASYQPDLDDYLAHRERWQALGLLQLPNLDRWESAWANMQQQYA
                     LPHLAYQIEPSITCPSTHCTQQWPFKQWPSLNFTLTPIRLSWSVGHESAIIPWIQQLQ
                     EAYRGMLLIRRCEWRLAEAKETVAVQCDVVMFNFPDVLTMLGQS"
     gene            complement(21643..>21991)
                     /locus_tag="VNZ84_04920"
     CDS             complement(21643..>21991)
                     /locus_tag="VNZ84_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66330.1"
                     /translation="ALQNTSAGRSHWQLKALEWNAGLPEGLPNEAIATDAPGVQDTLT
                     ATWLSSEPPVQAGAEWAELLSWLHNRPEIAKVEIIHPRDAAGNPQRLGDTRQLQPSGQ
                     QILKLYFKQGAGL"
BASE COUNT         5509 a         5969 c         5460 g         5053 t
ORIGIN      
        1 gctggcgtaa aaatgatccg gggggcgcgt tgtcgctgac catgtcggag ttcagacagt
       61 tgctcaagga cgaatacaag aaaaggtatg cttgagggcc agaaaacatg tcttggtatc
      121 gatcacccgt gctactcaat ggccaggtcg gcataccggc aattgcagcg attgtaatac
      181 ttcgccattc agcgcatggc gggcatataa caccacccca ttcgcatgcg cgagcagcga
      241 ctgatccggg acaagatggc gtaagatatt gagctgatcc aggctatatg gccccatgtg
      301 ttgcagcaca acgaaatcat ggttcaacac gcgcccatta ttttcaccgg cctttacttg
      361 cgtgtgtaaa ccatgctggt agactgccag ccacaactgg acaggcaggt gctgttgggg
      421 cgttgtgacc gttgtgcgga atgcaatctg gccgttatcc tcattcagtt gctgcagcgc
      481 aagtgtcaac tgggcaggtt gcatgttgat tttttgcagc agacgatcca gccggctatc
      541 actccatccg cggaaatcat atccattcag cgtaaattgc ggggtataca ccacattgct
      601 gccagtctgg ctagcctgag tacgctgttt gtcactaaag gcctcttggg caaaacggtc
      661 tttccagccc aaaccatccc agtagtccac atgaaatgcc agggcattga cctcaccttg
      721 cctggactga aggctactta accaacggtc agcgggcgga caactgctgc agccctcgga
      781 ggtatacagt tccagcaaag gtatgacata ttggccactt tccgcgtgac atgcggctgc
      841 gaaaacaggc gtaagccatg tttgcagaaa tatcagtgca accacacacc ctttgagttt
      901 catgattcat gctccggtat ctatcaaggt gcatattggt cgcacgcaaa tgatctacct
      961 tacatcaatc atttagttta tcccgttcgc catggcgact acacctgtag catggtagtc
     1021 gcctccagtg gcgtcaccgt gacgctcacc tcaagctgtt gccttcctcc acctaacacc
     1081 accccgcgca tgggagaaac gtcgccaaag tcacggcccc aggcaacggt aatgtgctct
     1141 tgctggacca ggcaattatt ggttgggtca aagtctaccc agccgtatac cgggcaatat
     1201 accgagaccc aggcatgtga agcatctgcg ccaaccaacc ggggctggcc cggaggcggg
     1261 tgtgtcagga tataaccact tacatatcgg cacgccaggc caagtgagcg cagtgcacct
     1321 atcatcaggt gcgcaaagtc ctggcaaacg ccacgccggc ctttgagcac ctcatccagt
     1381 ggcgtagata catctgtcgc ctgtgcatca aactcaaatt cggcatgaat cttttgggtc
     1441 agcgccatgg ccgcgtccag caaggaacgc tgtggcgtaa agcagttttt cgcaaatgcg
     1501 gccaaggctt cgctggactt gaccctgttc gtggcataaa taaaaggcac cgcttcaaga
     1561 tgctgcgcgc cttgtgcttg cagcatagcc tgcacttgct cccacgccgg actttgcatt
     1621 aatgcagtga taggcggacg cggttggagt tcaacttgga aacgcgatgt cacctgcaac
     1681 acctgatgcg tcgcgagcat cgtcaggtaa tgattgtgat tgccaaaaaa gtccagccgt
     1741 tgtgactgct ctgccggtat tggttcgcaa gccagcgtgt aggacacgca ggtttggaac
     1801 ggcgtctggc gcggcatcat atgcagtaac tgctgtgact ggatcactgg cagatcatag
     1861 ctatatatcg tttgatgttc gacttggtag cgcatgcaaa tcaccttaat acccgggtgt
     1921 caccagcgga ctggcataac taaaaaaacg catactcaga cggtcagaca aattcagtgt
     1981 cacgttatat gtttgattaa gccaatcagc caatgcggca ttacctgcaa taaagcattg
     2041 atccggatca agttgcagca aggtctgcca gcagccgttc aactcggtag caatcccgcc
     2101 tgcattatcc tgcgcgtcca tttcttcgag gtatttcacc agtccattga tctgaaacac
     2161 caccgaattc gggttgtttt catccatcag cagcaggtcc aggacaggca accattcagg
     2221 ttgtgccgca taccgtgcac ggtaagtgac aaggttattg gttacctgta acaaccattc
     2281 gagattacta tgctgcggca tgttcaatgc acgacttaat aacacgcaca tgaattgcaa
     2341 tcgctctatg cggcgaccaa gcgacataaa acgccagcca aggtcgcgcg tcatccagtc
     2401 catggcaaag ccggtcaggg tcacaaaatg attgatggcc tgctcaatat aagacagggc
     2461 atcttgcaga ctggcatgct ggtgtcgcgt aataaataac ctggacagta catttaccgt
     2521 acgccaatga tcgttagaaa tacgctcccg caggttaaaa gcctgctgga agagttgctg
     2581 tacgctagtg gccaaactgg tcgattgctg ggagaacacg gcctgaacca acaacggctc
     2641 aatatcatca tctgtccagg gttgccagtt ggcggactct tcttcgtctt tagggctggg
     2701 taacaattca aaccactggc acaggccctg catggtaggc cactctccgg cacgcgattc
     2761 tggtgtgtat tcaaccagtg cgcgtatcgt tgcccttagc gtgatggctt tttgcaggtt
     2821 acggaccgaa tagcggccat accaaaacag gttttctgcc atgcggctgg aaaggtaggc
     2881 attctcgcgc accaggtcct ggctggaatt ggttttacgc aacaagctga acgggggctg
     2941 gctatcctgc gacaacaccc aggtatcttt gctggtccca ccgcgctgca tggtgactac
     3001 gcgggcatct ttaccactgg ccacacgtga cagccctccc ggtaaaatcg cgtagccgtt
     3061 tggggtagca aacgcatata cacgcaaact caccgccagg gtacccagct gtggttgctg
     3121 gtgatggttg gtcaacacgg gcgcatgcga aatatccact tgctcctgcg caatgtaatg
     3181 gcctggctgc gcccgcaatt ttgcgatgaa agactctttc tggctagcat tcagatcctc
     3241 gccgaaaata ggtgatgaac gcagttgcgg ataagctggt ttaatcacaa gctcatcaag
     3301 atgctgaatc acatattcca gtgccgcagg ctcaccgcac caccaggtgg ccaccgatgg
     3361 catgcgcaaa gcttcaccca gtaatgattg acataaattg ggtaaaaagc ccagtaaagc
     3421 accagactgc aacacattgg atcccagcgc attcgcaatc agcacattcc ccaaacgtgc
     3481 agcctgggtc aggccggcca cgcctagcaa ggagtccgca ttgagctcca gtggatcgca
     3541 gtaatcatca tcaatgcgac gcaaaatcac atgcaccggc ttaagtccgg cgatggtttt
     3601 gagccagacg atgccgttac gtacagtcag atcgctccca tgtaccaatg gataccccag
     3661 ataacctgcc aggaaggact gctcgtgata ggtttcgtta tagggccctg gagtcaggat
     3721 cacaatgagc ggcggctccc cctgtcccag cgcggtcacc ccagggacag tctgtgcctg
     3781 gttagccgcc acacgatgcc cccagtgcgc aagactgtca cgcatgctgg caaagaaccc
     3841 actcaagcgc tcgatgttga ggtcacggaa caactcagga aaagcgctag aaataatggt
     3901 gcgattttcc agcgcatacc cagcaccggt aggcgtctgt gtacggtcag acaccaccca
     3961 ccactgcccg ttgggcgccc gcgccaggtc gaccgcatag ctatgcagcg ccatgttgtc
     4021 gtaatgcggg ataccgacac aagagggcaa aaagccgcta tgcccataaa tcagggcggg
     4081 cggcaattga ccactggaca ataagcgctg ctcgccataa atatccagca acactcgatt
     4141 gagcaaggtg gcacgctgcg ccaccgaggc ggcaatatgc tgccattcct cctgcgggat
     4201 aatcaatggc agcacatcca ggtcccaagg ccgttgcacg ccatgggtat ccgcataaac
     4261 gttgtaagtg acgccgttat cacgaatctg gcgctggacg gcctctatgc gtttgcgcat
     4321 ggccgtcggc tcttcggtct ccagttgcga gaccaatgcc tgccaatgcg gacgtgcctg
     4381 accgctgcta tccaacagtt cgttgtagcg gtcagaaaaa taaagctggt taagcactga
     4441 ttgcatattg aataaagggt cagtcacaac acattacatg acaagacact gagtgtaaca
     4501 ggatgttcac agcttggcct gccagcgcag gtcaagcgtg aatggaaaac gggggttcgg
     4561 tggttctttt accacatcca cccggcccgg tgaatgccca tgcgaccaga agcgtgcaaa
     4621 tcgacgcgcc tccgcttcat tggcattgac cgggaaggtg ctgtaattac gcccgccggg
     4681 atgcgagaca tggtatgtgc atccgcctag agactttgca ttccaggtgt ccacaaggtc
     4741 aaacactaac ggggcttgta ccggaatagt cggatgcaac gctgaccaag gactccacgc
     4801 ccggaaccgt atgccagcaa caaactcccc tgcattgccg gtaggttgca gcggtaactg
     4861 cctgccgttg caagtcaccg catgacgacc atcggtcagg ccgcgtacgc gcaactgcat
     4921 acgctcgacc gaagaatcaa catagcgcgc cgtgccacca ccagacattt cctcaccgag
     4981 cacattccag ggttcaatcg cctgccgcaa ctccagctcg atgccatgat aggcaaccgt
     5041 gccatagcgc ggaaacctga actcgacaaa cggatcaaac cagcgctgct cgaatgcata
     5101 tccggcttca cgcaaatcag ccaccacatc cgcaatatcc tgtgccaaaa actgcggcag
     5161 catccagcga tcatgcaact ctgtgcccca atgcactagt ttgccggtat aaggtgtttt
     5221 ccagaagcgt gcaaccagtg cacgcaataa taggctttgc agcaaactca tgcgcgcatg
     5281 cggcggcatt tcaaaggcac gaaactccac cagccccaga cgcccggttg ggccatcagg
     5341 cgaatatagt ttgtctatag aaaactctga acggtgtgtg ttgccagtta aatccaccag
     5401 caggttacgc aacaaacggt ctaccagcca gggctgctgg ctttcttttc cgctagacag
     5461 cattttctcc atttgctgga aggcaatctc cagctcatac aggttatcgt cacgcgcctc
     5521 atcgacacgc ggagcctggc tagtgggtcc aatgaatgtg ccagaaaaca ggtaggacag
     5581 ggccgggtga ttttgccagt aagtaatcag gctgcgcagt acatcagggc gccgtagcat
     5641 cgggctatct gctgccgtct cacctcccag ggtcacatga ttcccgccac cggtaccggt
     5701 atgacggcca tccagcatga atttttcagt tgccaggcgg cataaacgcg cctcttcata
     5761 caacgtaccg acattcgcaa ccagctcttt ccagcttttt gccggatgga tattgacctc
     5821 aatcacgcca ggatccggcg tcacgctaag taatttcagg cgactgtcac ggggcggggt
     5881 atagccttcc aaccatagtt tcaatttgag atcactggcc gtctgttcaa ttgcgctcag
     5941 caaatccagg taatcttcca gccgggtcac tggtggcatg aatacgtata accggccttg
     6001 cctgacctga acgcacaatg cagtatgtat cacttcgcgt gcagtgggtt gagtcgtctg
     6061 atgcttcacc ctcactttgg gcgtcttagc ctcgtccaaa gcaggttttt ctgcaaacgt
     6121 gtcgggggca aactccggct caatgtctgc aggcaattgc cagggcagtg aagacaaggg
     6181 caagcgcaag cccataggcg aatcaccacc tagcaggtac aaatgctcac gccttaacgg
     6241 ccatttgctg gtcaaccatt caccagcact gcggctttcg tctggcttaa gcggcattat
     6301 gtaacctacc acctgcccca ggccctgact gagcaggcta gccagacgtt tgcgctcggc
     6361 ggcatccttc aagtcggctt tgagcggatc aatattggca ggcaactgct gctcctgctg
     6421 tgccagactc atgacatctt cataggcagg catcacacac gccttgggaa aacccaacat
     6481 atccgccaag tgggtggcaa acacctgcgc ctgcttaata ccgtaaccat ccccagggcc
     6541 ttccgccgag aacaggctgg cgtcgcgcca tactggctta ccatctggcc gccagtaaat
     6601 attgacggcc caccgcggca acggctcacc tggataccat ttcccctggc catagtgcaa
     6661 gaatccatga ggcgcgaagt gatctttcaa acgtaatgcc agctcacccg ccaacttgcg
     6721 tttatgttca cccaaggcgg cggtattcca ctgtgcgcca tccatgtcat cgatggagac
     6781 aaatgttggc tcgccccctt gcgtcagccg cacatccatt ttggtgagct gtttgtctac
     6841 ctgctcaccc agcttaagaa ttgcctgcca atccttttca ctatatggct tggtcacacg
     6901 cggatcttca tggatacggg tgactttcat ttcatgactg aacgtcacct ctgccacatc
     6961 ggtaaagcct gtcacagggg ctgctgaaga tggcaatgcg gtacacgcta gcgggatatg
     7021 tccttcgcca gccatcaagc cagacgtcgg gtccagcccc acccagccag cgccaggaat
     7081 aaagacctcg gtccacgcat gtaggtcggt aaagtcctta tcggtgccac tcggtccatc
     7141 cagtgcctca acatcggctt tgagctggat caggtagcca gagacaaacc tcgccgccaa
     7201 gccaagattg cgcatgacct gaaccagcaa ccaggcagaa tcacggcaag agccggtgcg
     7261 tttttgtaac gtgtcttccg gcgtctgaat tccaggttcc atgcggaccg tataaccgat
     7321 atcgccttgc agtcgctggt tcacagctac gagaaagtcc acaatgccaa tatcgtgctt
     7381 caccacctcc gctcgcacca ccgccatcca tgcttccagc aaaggcccca taggttcagc
     7441 ttgcaaataa gcggttaatt caaatgcctg ttgctcggta tagttaaatg gaaaatgctc
     7501 ggcatatttc tcgacaaaga aatcaaacgg gttgatgacc gtcatatctg ccactaaatc
     7561 gaccgtaaaa tcgagctcgc gcaccatttc ggggaataca aagcgggcga tgtagttacc
     7621 gtaaggatct tgctgccaat tgataaagtg cttttcggga ttcactgtca atgaatatga
     7681 caacactggc gtacgcgcat gcgcggctgg ctttaggcgg acttcgtggg gagagaggtt
     7741 gaccaggcgg tcgaacttgt aactggtgcg gtgatgtaac gcaacgcgga tagccactgc
     7801 cactcctttt agaattatgg ttttaggcag tgagatgcag gaaatatgcc agctacgcaa
     7861 ggatcactta agcatagctg gtgaacaatc attccatcat aagtcgcgtt ctgaccaggc
     7921 cgttatttgc taccaccaac caggtttttc acggcactga caacatcgcc aggcatgacc
     7981 acaatcttgc tgttgctgga atccgccatt ttttgcagcg actgtatata acggtcgccc
     8041 aacaaaaacg ttgctgaaga attattttcc ttaacggctt cggcaatcat cttgatcgct
     8101 tcagccgaag ccttggcgcc aaccatttgc gcttcagcat ccttgcgtgc ggcttcaagc
     8161 ctcgcttcgg cattcaaaat cagggactgc ttctggcctt cggcacgcgt aaccaccgcc
     8221 acacgctccc gctcggcgct ggcctgttgc tccatcgcgt gctgcatatt ggctgatggc
     8281 ttgatgtcct ggatttcgac actttttact gtcaggcccc agtctaatgc ctcatcggca
     8341 atcgcctctt ttagctccgc tttgatccga tcccgtgaag tcagggcatg gttcaggtcc
     8401 atgccgccaa taatcgagcg caaactggtc tgcaccatat tacgcatggc ttcgcgaaaa
     8461 ttttcaatgc catagacagc acgctcaata ttggtcacct tgataaaggc gactgcattg
     8521 gccaggatca ccgcgttatc gcgtgtaatg acctcttgct caggcacgtc cagaataata
     8581 tccttggtcg tcaccttgta gctaatcgtg ctcaatatcg ggttaatcac atgcaaaccc
     8641 ggcgttaaaa tacctgcaaa cttgcctagg cgctcaacca cccactcttc gccctggggc
     8701 acaatacgta cgcctttaac aatggcaata atcgccaata tcaatatgat gatgctgatt
     8761 tccatgtgta ttcctctgct gaaaaatatt gagctgccgc atgtagcaac cccaaaagcc
     8821 ttgggctagg ggctagtgtc gcttaatgtt gtgtcagtgg cgcaacacgc aggcaattgc
     8881 cttccactgc catgattttg gcaggctgat tgatagcgat ttcaacgtcc gcatagacgg
     8941 tccattcttt gctgcccatc acgccttggc taaaacgaat tttgcctggc tgtgccgggc
     9001 tgacggcgac ggtgatcaca ccctgacgac caatctcctc accctgcgcc tgcccgacac
     9061 gcggggccgg atgggcctgt tcccgcttgc gccagatagc cacggccaat acggaaaaaa
     9121 tggcatagat gacgatttgc caggcaagac taaatgccgg gaataaccag gaaaacaacg
     9181 ccataaccaa ggcggcggcg cctggccaaa gcaaatacaa agtaccggac atcatttcca
     9241 gacataataa aaccaagccg ataacgcccc aactccagac cagttgcacc gtgctcacct
     9301 cactattgtt tttaatatat cctatcagaa aagaacgtat ttttgctgcc tattgcgtac
     9361 atgggaacaa tgctgattgc attagcatgc cctaggctat ccgtttaaaa cgttaccaaa
     9421 cagggagaat tatggagatc gtcaccttag tcatattcgc agttttaacg ctgtatatca
     9481 tcatgctata taacagtcta gtcagcgtta aaaacaacat ttccaaagcc tggtcgaaca
     9541 ttgaaatact gctgaaacaa cgtcatgacg agttgcctaa actcattgat acctgcaagc
     9601 aatacatgaa gcatgaacaa gaaaccctgg aaagggtcat gttagccagg tcacaggttt
     9661 ctaaagcgca gcagtcacac gatgtggatg ccttgggcac tgccgaaggc gcattgcgct
     9721 caggcttagg tcatttgttt gccgtggcag aagcgtatcc tgaattgaaa gcaaacgaga
     9781 atttcctgca tctgcaatcc cgcatcagcg ggctggagaa tgccattgct gatcgccgtg
     9841 agttttacaa tgaaagcgtg aatatcaaca acgtacgtat cgaacaattt cccgatctgt
     9901 tagtggcaaa attattcaat tttccccgct ttgagctcat gcgttttgcc agcgaagaat
     9961 tgcgagacat cgatatcggc aaacgcttta acagttaagt tacacgatgt tgcagttttt
    10021 acgcggtcac gatagcgacg cgatcacgtc agttggccat cttaccttgt tgctgatggc
    10081 catttatgcc cataatcaac gggtatggat aggctgttgc agtttgatcg gcctcatcag
    10141 cctctgggcc tgggctgcca actaccaaag atgccgcgtc atctcagata caccgacctc
    10201 acgcattgcc tctgctgcac aaggctatgt tgagctgtat ggacgcgctg cgatgacgta
    10261 caacaacctg ctcattagcc cctacagcca cacccgctgc atttggttcc gttatcgcgt
    10321 ctatgaaaaa gaagacaaag gctggcgcca ggtcactaac cataccagtg acagtacgat
    10381 agagattacc gatggcacgg gcagttgtgt gatcgatcca gaccatgcag aagtcatttc
    10441 gcccgatcgc cgggtggtaa cccaaggcag ctataaatat gtggaggagc tgctgagaag
    10501 cgactccgtt tatgccttag gcgagttctc gaccataggc ggcgccaatt ctgtactcga
    10561 cttgaagtcg gatgtcgctg ccttgctggc cacctggaag gaaaataaac aaactctttt
    10621 gcagcgcttt gacttggatg gcaatggcga gattgatatt aaagagtggg aattagcacg
    10681 cagggcagcc gtgcgtgaag tcgagaagga acatagagat atccgggccg cagatggcgt
    10741 ccatgtgatg cgcgcaccca agaatggcca gccatttcta ttatcaaacc tgtccccaca
    10801 aaagttacgc aacatctatc gatgctgggg tgccgtgcat ttaaccattc tctttttggc
    10861 agttgcaggc gcactatggt tctggaaagg ccatacgttg accgaactgt ttatttaagt
    10921 gtctgaaaaa cgcacaatct aagaaggcgt gttatctcag agtggattgt attcggtagc
    10981 attaaaccta aggtcaggca gcgctagaaa tagctgacca tctacccaaa ccagaccgcc
    11041 atcctgttag cataagtgcc cccgaacaac ttaagaatat ttgaaggcat ggtatcacta
    11101 cttgtgtcaa aatggggcaa tgactcaacc gcctacccgc ctcacgcttg ccaccgaccg
    11161 ttacgaacgg ctggcacaaa tccgtgactg ctttccgcaa gccaccgggc cagatctcgc
    11221 cgagacatgg caaggcgggc gtcagatggc attgcaacgg ttatcagcca ttgatgccgt
    11281 ggcttatggc cgcagtcgca accatctaga tggctcagtg acacggctct ccccttattt
    11341 gcgtcatggc tgcctgtcat tacaggaagc catcaatacc atccagcaag actttggcgc
    11401 gcaaggcatc aagctgattg ccgaactcag ctaccgggat tatttcaggc aggtctggta
    11461 ccgttttggc gatgcgatcc agcaaccctt ggagccatcc aaagttatac caggcgaaaa
    11521 cagcttgcct gagtttattg ggcagggatt taccggactg gtgtgtatgg atgagattgc
    11581 gcgcgccctg caacaggagg gctatgtaca caatcatgcg cgcatgtggt ttgccgccta
    11641 tgtactgcac tggttaaagg ttgattggcg ccaagcggcg gactggtttg aacaacactt
    11701 actggatggt gaccttgcct ctaatcatct ttcatggcaa tggatagcct ccagctttag
    11761 ccataaaccc tattatttca acaaagaaaa tatcatcaaa tttggcggtc aacgctggtg
    11821 ccagcaatgc cgggtgcgct gcccgtttga tgccagctac gaagcgttgg aacaacaact
    11881 atttaccacc tcctccggag ctaaaacgac gcttcctaac gtcgtcacga gagccgcaca
    11941 tcctcaacaa gcggacgatt tgtcacaagc ggccaaggcc aatacgctgg tctggcttca
    12001 cgatgaaatg ctctccccgg aacatccact actgactacc aagggggctg tggcctttgt
    12061 tttcgatcct gcactttacg ggcaatggcc cttgaaacgg ctgcaattta tggctgactg
    12121 cctgagtgaa attccacagg cagaaatatg gatggggagc acaaacgaag tgctggcagg
    12181 cctctcgcag gagcagatca tcagtcagga gacaccaaac accgctatta aagcagtggt
    12241 gaaagatgcc aggctgacct ggataaaaga gcccagttta tataacacct cctttagcga
    12301 tcatgagctg atgcgattta gccgctactg gaaaaaagtc gaaccactgg tatttgcgga
    12361 aacccatgca ggatgattca aaaaataaaa gccgccagat atccgcggct tttttaaaca
    12421 acctgcctaa ttaacgctgg cggcgacgct gtatccaagt catcagcaac aagccagaaa
    12481 ccagcagcga tacattgcct ggttctggca cagcgctcac tgaaagcgcc tgatttacaa
    12541 acgcgctacc gttcaaatcg gcatcggtaa acttaaacac gaagaacttg ttggcgcctg
    12601 ctgtcgccgg caaaaagtca ttatcattcg ccacatataa ggtgtgataa agcacaccgc
    12661 cttccatgat gtcctcgcca aaggtcatgc cttcaagctt ggatggaata ttggtattac
    12721 tgatacctac atctgtgagt gctgacttta agtcaaggaa caagcttttt tgtactgcat
    12781 atcccagtaa gttactttgg ccgctgatac cagccccggc attgagagag ccaatatcta
    12841 ccgccagatc accactgaca ccatcgccat ttgcgtcatt taaatcaatt ttgtaaatgc
    12901 gtttaacgtc tgccttagaa tcatcgccca ggcctttacc atcccgttcc agcacgagaa
    12961 aggtgtgatc attgaccgcc agaatttcgc tgctgttata gcttttattt ttatccgaga
    13021 gaaagttgtc atacacaaat tgcttggtcg cgccggttac tgtgtcaatg gtcacgatac
    13081 ggttggtgcg gccgccatca ccgccatcct gaatcagagg gctttgctca aaaccgacca
    13141 gcgctttacc atctggggta atggccaggc cttccatgcc tttgttggca actcggccac
    13201 tgctgttacc gctgatttcg tttgcgccct ggctgctcaa actgctggcg gtgaaggcag
    13261 atggcaggtt gtacacattg gttctggcac cggtagcacg gtcaaactgg tacacataag
    13321 ggccatattc atcactgata taaacaattt taccatcgtt cgacacacga attgcctctg
    13381 gatcgaaacg gccgttattc gggttactgg agttgcttcc tgccaaaaag ttgtcagagc
    13441 ggccattgaa atagtacttg ccggcatcga cagttgcgcc actccccagc gtatagctgg
    13501 tgccgcccac cgaacctgtc ccggctgaac ctgacccata attcagcggc gtattgctgt
    13561 ataacaatgt ggtgccagtc aaggtcggcg tcaggctgta actgtagcta ccgccattaa
    13621 atgtctgggt caaatcaagc ttgagtgttt gaaagcgcgc aatgtaagaa gtcgtattgt
    13681 cgaccagcgg attgtatacg gtggcattcg gcccacggtc aggcagtgcc aggaaggtat
    13741 taccccctgc ccaagccagg ccggaaccca tgccgcccaa tacattctgg tgatccactc
    13801 cattttccag tacgccggaa aagctggata agtccgtggt gccagacagc gcaccaaacg
    13861 tgatgagtga aggggtagca ttggccagtg taggcaacgc cagcgagatc gccacggtca
    13921 tggcagccaa tttgaaagtc atgtcaaaac tccagaataa gaaattttga ctaaattaat
    13981 cccatgcagt gaattaaata tgacaggact agcgacaccc ggctgcgagc accagaacag
    14041 agggagtgct gtggtaaaat cacgcgcatg actacttcta atcaaaactc caatccagcg
    14101 cgtgaactcg ccaaatcgtt tgaccctaaa tccatcgaaa gccaatggta tgccttctgg
    14161 gaaagcaaag gcttttatgc cgcaggcctg aaccctgacg tccaggataa cttctgcatc
    14221 ttgctgccac cgccaaacgt cactggcacc ctgcatatgg gtcatggttt taaccagacc
    14281 attatggatg ccctgacccg ctaccaccgt atgcgtggtg acaataccct gtggcaacct
    14341 ggtactgacc acgccggtat tgcgacacag attgtggtag aacgtcagct ggatgcgcaa
    14401 ggtgtgagtc gccatgattt aggtcgcgaa aaattcctga aaaaagtgtg ggaatggaaa
    14461 gaatactccg gcggcaccat caccaagcaa atgcgccgcc tgggcacctc ccctgactgg
    14521 ggccgtgaac gcttcaccat ggatgcaggc ctgaacaagg tcgttaccga aacctttgta
    14581 cgcctctaca acgaaggcct gatttaccgc ggcaaacgcc tggtgaactg ggatgtgaaa
    14641 ctcggaactg ctgtttctga cctggaagta gtacaggaag aggaagacgg ttccatgtgg
    14701 catatcaact atccgctggc tgatggctca ggccagctga ctgtggcaac gacccgccct
    14761 gaaacgatgc tgggtgatgt agctgtgatg gtgaatccgg aagatgagcg ctatgcccac
    14821 ctgatcggca agcgtgtttc cctgccttta tgcgatcgtg aaatcccaat cattgccgac
    14881 gaatatgtag ataaagagtt cggtactggc gtagtgaaag tgacaccagc gcatgacttt
    14941 aacgacaaca tggtcggcca gcgccaaggt ctggacaaaa tcgtcatcct gaacctgcaa
    15001 ggttttgtcc ctgaaacagc agaagtctat gctgctgatg gtagtaccaa agcgccaatt
    15061 gcgttgcctg caaacttggt aggcttagag cgttttgcgg ctcgcaaaca agtggtggct
    15121 gatctggaag ccggtggctt tctggttaaa gttgaaaaac acaaattgaa agtgccgcgt
    15181 ggtgaccgta ccaatgtagt cattgaacct atgctgactg accaatggtt tgtggccatg
    15241 agcaaagccg gcgaagacgg caagagtatt accgaaaaag cgctggaagt cgtggcaaat
    15301 ggcgaaatca agttctatcc ggaaaactgg gtaaacacct acaaccaatg gctgaacaat
    15361 atccaggact ggtgtatttc acgtcagtta tggtggggtc accagattcc ggcatggtac
    15421 agcgatgatg gcaaagtcta tgttgcgcat gatgaagcag aagccaaagc catggctgct
    15481 aaagatggtt ataccggtgc actcacccgc gacaacgacg tactggatac ctggtactcc
    15541 tccgcattgt ggccattctc taccttggac tggaccggcg acgaagcgat tgacgctcaa
    15601 aaccaggcgt tacagcaatt tctgccttct tcagttttag taactggttt cgacattatt
    15661 ttcttctggg tagcgcgcat ggtcatgatg accaagcaca ttaccggcaa gattccgttc
    15721 aagcatgtct atgtacacgg cttgatccgc gatgcggaag gccagaaaat gtccaagtcc
    15781 aaaggtaatg tgctagaccc tatcgatttg atcgacggca ttgacctgga atcacttgtg
    15841 aagaaacgca ccactggcct gatgaatcca aaagatgcag agaaaatcga gaagcgtaca
    15901 cgtaaagaat ttccggaagg gattgctgct tacggcacgg atgcactccg cttcaccttt
    15961 gccagccttg cttcacctgg ccgcgacatc aaatttgacc tgcaacgctg cgaaggctac
    16021 cgcaacttct gcaacaagtt atggaacgcc acgcgtttcg tcttgatgaa caccgaaggc
    16081 caagacaatg gctttggcgt ggagagctgc gaagacggtg gccgcaagtt cagccaggcc
    16141 gaccgctgga ttgtcagctt gttgcaacgt accgaagcag aagtcgaacg tgcgtttaac
    16201 gattaccgtt ttgacctggc cgccaaagcc atttatgagt ttgtctggga tcaatattgc
    16261 gactggtacc tggaaattgc caaagtgcaa atccagggtg gcagtgaagc tgaacaacgc
    16321 gcgaccagac gtactttgct gcgcgtgcta gaaaccatcc tgcgcctggc gcacccgatt
    16381 atcccgtttg tcactgagga aatttggcaa accgtcgcac cgatgactga cttgaaagac
    16441 aagtcacgcg gcaccgaaag cattatgttg caggcttacc ccaaagccga cctagagaaa
    16501 atagacgaaa cagcagaagc ctgggtggct acattgagaa cagcggtgga agcctgccgt
    16561 agcctgcgag gcgaaatgag tatttcaccg gcagccaagg tgccactgat cgccgctggt
    16621 gacgctgcac aattggcagc ttacgcccca tacttaaaat cattggccaa gctggaaaac
    16681 gtggatatcg tggcagaact gccagaagcc gacgcgccag tgatgttggc gggtgacttt
    16741 aaattgatgc tcaaggtaga aattgatgtt gccgccgaaa aagagcgcct gggtaaagaa
    16801 atcgcccgct tgcaaggtga aattgccaaa gcgaatggta agttaggcaa tgaaagcttt
    16861 gttgcccgtg cgccggtcgc tgtggttgat caggaaaaag cccgattggc ggagttcagt
    16921 gccaatctgg aaaagctaca agcgcaactg gcaaaactga agtagttttc atcacaaggt
    16981 actccgttgc cagatcaggc gacggtgcca cacttgtggc aaaacttggc aaaggcattt
    17041 ttgcgcgatt ggcaggcatt gcaataattg tgcaagcaaa tgccgcaatg cgtgcaaaaa
    17101 tcattttgct catttttcag gtctacagtg cgttcgcagc ccgggcatac atttttagcc
    17161 aatctcgcta gtgcagtgtc atacccaagc tcttcccgcc gttgaaattc gggctgtgac
    17221 tccgctagtt tttgctgctc caaataattt tgcaacgcag ctatcgcata ttttccgcct
    17281 accaccgtca ataaaatacc tacggtatac cgtacatagc cgccataatc cggtaaatat
    17341 ggcaccaact caacaaagaa ggccatcaga gcaaaaataa taaatcccca cacaaatggc
    17401 cagtattggg attgccgctt ttttgcaaat aaccaggcag ccaacgccaa taaaggtaaa
    17461 gtcagcaaca agcgatacaa aaatacgcgc attgcctgcg cctcataggc gtcgtctaac
    17521 tgctggcgag ctgcgtcctc caggctattc acctgtgtgt tcaattgcgt ttcatgctgg
    17581 gaaatattcg tttgctgctg ctggatatct tcaatttccc gctcagcatc ccgttccagc
    17641 tttttgagcg catccaatgc ccgcgtccgg ctgaccaggt catccacttc atccgtcgta
    17701 tcccgggttt tgacccagtt atcaaaagta gtgcgcgccg cctggtaatc tttttgccgg
    17761 ataatgagtt gtaaattgac ttgttccagc tgatcagcaa gtgtctgtcg ctgccaggca
    17821 agcgttttca actctgtacg tagtggatca atttcggcgt ggtttacaaa atgttcggtc
    17881 gtgtaatacg tttctacacg cggtaaatca ccaatgatgc ttttgcccaa acccgtcaaa
    17941 aaccaggcaa aaataatggc caccatccat agtgcacggt taaaccactt ttcggataaa
    18001 cgaacgcctt tactcatcag cttgcccttt caacggtatg catggatgct aacgggcttt
    18061 tggccacaca gcaatagcac acttcgcatg cgatgctggt ctgactagtg gtctgccgct
    18121 agaatgcagg ttggcaagat taaacaattg tgtacggatg tgtgaaatcg acgaagaaaa
    18181 cccgaaagaa gtgttatgca gctgtagcgg caccacccgg ggattaatta tcgaactggt
    18241 caaacagggc aaagaccttg agggcatttc gcgctattct ggcgcactca gcggctgcgg
    18301 tggctgcgaa tgggagattt ccgagttggt caataaactc caggccgggg actcaacgcc
    18361 taaaccttaa tgaccgcaca ccgcacagga ggcgtctttt ttcaggcgta tggtgcgcca
    18421 ctctgcactg agtgcatcca gcaacaataa tttacccttc atggtttggc cgatattcag
    18481 caaaattttg agcgcttcgg cagcctgcgc agtacctatc atgcccacca atggtgcaaa
    18541 tacaccgtta tcggcacaac gtaattcctg gtcatccccc acatccggat acaggcagtg
    18601 ataacaagga ctctcgtcat gccggaaatc gaagacactt aactgcccct caaaacggat
    18661 ggcagcgccg gaaaccaggg gcgttttatg gtcaaaacaa agctggttca acaagtagcg
    18721 cgtggcaaag ttatccgagc aatcaatcac gacatccgtt gctgccacac gacccgccaa
    18781 cgcctcttca ctcaaccggg tggtttcaac atgcacaatg atttcaggat tcaggctggc
    18841 aattgtcgcc tgtgcagata acgccttgtt ttggccgacg gctgacgtag tgtgaataat
    18901 ctggcgctgc aaattggtca ggtctacctg gtcaaaatcg cagatagtca aggtgccaac
    18961 acccgccgca gccaggtaca tggccaccgg cgcgccaagc ccacccgcac cgatgatcag
    19021 cgcatggctt tgtgtcagtt tttcctgccc cgcatattca atctgcggca gcataatgtg
    19081 ccgactgtaa cgtaacaaca aattgtcttg catcagaatt ccgttttttc agggcagcct
    19141 ttgcgcctgc accatttgct ggatcaatac tttgggtgac agcatgatca ggcggtcatc
    19201 aaacactgca ccacccgtct gctgttggga tttatcaaca aacacaaggt cgccatccgc
    19261 attttcctgc tcatcaggat gcgttggcat tgccatgaca taggtttgcc caccagacac
    19321 ggattgtatg ccacccagcc cgttttcgcc atgcgaaagg atgatcgccg ccgggcgact
    19381 gggcgtactg gtgaccgccc cagcctctcc aatcacttgg agattactgg cgctctcagc
    19441 agcagagatt gaaaaccact gggcatggtg ggtgaatgta ctatccaccc gatagcgaaa
    19501 aaagcgaccc caggcatcag aagccaccaa gcctaattgc gcccagggca gcaccccttc
    19561 atcgctcgcg cagctctgat tcgccaatac attttcccaa ccatccggtg gttggtctgt
    19621 atccgggcat ggcaggtaat gatagatcat tgcgtgtccg agaagcgcct gctgtgcttg
    19681 cactaagcgc tgctcggttt gctgatattt ttgcagccca tgttgtacac gcaatggcgt
    19741 cattaaccag cccatcatca aactaagaat cacgagggcc agggccatct ccagtaaact
    19801 gaagccagga ataaatttgt gcgctggtcg gcactgggta caaaacgtgc tcatggctgc
    19861 atcacccgat ccaggatgtc gttgcttggc aaagtcagtg ctgaggcatt ttgtacctgc
    19921 aatatttgta aagtagatgc ctccaggtac gcattcagat tccccttatc agccacattc
    19981 tctctgacct gccccggtag ccgctcacct gccacgacca gagtcgcttg tgttggacca
    20041 gcctggtaaa acacttgctc ccgccagcca ttttgctgga accaatgatg cctggcatca
    20101 ccatccagca gatgctgtgt ggctccaggc caatgtgccg ccgcatccag gggcaaacgc
    20161 ccaatatttg ccgtagcaga aagacattgt ccggcaggca tcgattgaaa tcccaagcaa
    20221 cggtcatcct ctgtcgcagc gggagatgga tgggtcgtgc acggcgctac tgcacagcgc
    20281 aggctgcgct tgagttcact caaaacctgc ttttgcattt gctggtgcat gctccctgtc
    20341 gtgatgggcc aaaccacatc attgaagtga ggactggaag ccgccatgac aaagccattc
    20401 actgcttttt ctacagggtt ggcattatcc aggccatgga tatcaagata atgcttggct
    20461 atttcgctgg aagcttcttc acgttgctga acccagccat cttcacgcat caatggctgc
    20521 atgggcgcaa tcagcacagc gacaagccca tcaccttgag tagcatccca gagcacacct
    20581 tccgcagata gcatgctcca ggtaccagaa gtatcaagat ttaagggcaa aatgcgtgga
    20641 ttgtttttgt aacgctcaga gacggcatac cataatcgct ctccactgcc atcgcgcaca
    20701 tcgccaatac ctagcgtctt ccatggcaat cgtccgaggc gggccgtatt actgcatgaa
    20761 gtttcagcaa taccatcatt attacggtca ggacatggca agtcgccagg ccgcggacaa
    20821 tttgattcgc attgcgtcac acctaatggc tgctgggcat aagccaataa tgcctgttgc
    20881 gccagtgcaa gcgacgtgat cgttttctgc atcatgaggt gcctggctga aagggtgttt
    20941 gatgaggtat acaacatcca tccacccgca cctgacagca ataacaatac aacccaaact
    21001 gccacgcctc tctgccaaat cgacgtgtgc atcatgactg acccagcatg gttaacacat
    21061 caggaaaatt aaacatcact acgtcgcatt gcacagcaac agtttccttt gcctctgcta
    21121 gacgccactc acagcgccgg atcagtaaca tcccgcgata cgcctcttgc agttgctgta
    21181 tccatggaat gatggccgac tcatgaccta ccgaccagct aagccgtatg ggcgtcagtg
    21241 tgaaattaag tgagggccat tgtttgaacg gccattgctg agtacagtga gtacttgggc
    21301 aggttatcga gggctcaatc tggtaagcga gatggggtaa ggcatactgc tgttgcatgt
    21361 tcgcccaggc cgactcccaa cgatccaaat tggggagttg cagtaaccct aatgcttgcc
    21421 atcgctcacg atgcgcgaga tagtcatcaa gatcaggctg gtagctggca tattgcgcgg
    21481 attgctgctg ggaatgccgc cacttttgct cagcctgcgc caacgctttt tcggcaccag
    21541 actgccgatg catgatgata gccacaaaga cggcgagaac acccaaagca gctaaccagc
    21601 cgatgaatac tccccgcaaa gtgcgccagt aactattgtc actcacaagc cagcaccttg
    21661 cttgaagtac aacttaagga tttgctgacc agacggctgc aactggcgtg tatcaccgag
    21721 ccgttgtgga ttcccagctg catctcgcgg atgaataatt tcgacttttg caatctcggg
    21781 ccggttatgc aaccagctta ataactccgc ccactctgcg cctgcctgga caggaggctc
    21841 actgcttagc catgtcgccg tcagcgtgtc ttgaacacca ggcgcatctg tcgctatggc
    21901 ttcatttggt aagccttctg gcaaaccagc attccactct aaggccttta attgccagtg
    21961 actacgaccc gccgatgtat tttgcagtgc t
//
LOCUS       DATMSK010000144         4120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_292871,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000144 DATMSK010000000
VERSION     DATMSK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292871"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VNZ84_04925"
     CDS             <1..1039
                     /locus_tag="VNZ84_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU66331.1"
                     /translation="SQPCTAPATCSPPVVENHHETRLGTWRVLNYAQLSLHFPLSALL
                     FCILSSAIFAGILAWSISHPVKLLLDHLKSAAKGDFLIGVSPKITASHDEFSELGEAL
                     DLMTLRIDNMIKSQSNMLHHVSHELRSPLARIQMAVGLVMQNPKKMQSSLQRVELEAV
                     RMERMISELLEIFRFESGMQKLQKTEVDLKGLLTGIVGDVLFEKHEAKLRLDMPGDPV
                     VVEGQLELLQRAIDNVIRNAVKYGPEEGVVAIELQHSARTGEVILEVQDQGEGVEPQE
                     LEQIFRPFVRGRRASQVDGHGIGLAMTKHIVEAHGGFVKAANLNPHGFMIRIHLPAPQ
                     KHHAQETKKWM"
     gene            1091..2629
                     /gene="lysS"
                     /locus_tag="VNZ84_04930"
     CDS             1091..2629
                     /gene="lysS"
                     /locus_tag="VNZ84_04930"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWU66332.1"
                     /translation="MTAQHANPDSTTESVVDENHVIAERREKLRLLREQAHSAQAAVF
                     PNDFKPEHKAAALHAAYAEVDKETLDPQELPVVVAGRMMLKRVMGKASFATIQDSSAR
                     IQLYIARDEIGEELYESFKHWDMGDILGARGRLFKTRTGELSVHVTGLRLLTKSLRPL
                     PEKFHGLQDQEIKYRQRYVDLITSEETRTTFKARSKVVAAIRQFMNDNEFLEVETPML
                     HPIPGGASAKPFITHHNALDMQMFMRIAPELYLKRLIVGGFERVFEINRNFRNEGLSV
                     RHNPEFTMMEFYAAYTNYQWLMDFTEQCIRAAAIAANGTAVLSYAGKEVDLSKPFERL
                     TIIGAIQKYAPQYTLDQLNDVDFLRKELLKFGVKPFDHAGLGALQLSLFEETAESQLW
                     QPTYIIDYPVEVSPLARASDKHPEITERFELFITGREMANGFSELNDAEDQAARFHAQ
                     MKAKEAGDQEAMYYDADFIRALEYGMPPTGGCGIGIDRLVMLITDSPSIRDVILFPHM
                     RAES"
     gene            2788..>4120
                     /locus_tag="VNZ84_04935"
     CDS             2788..>4120
                     /locus_tag="VNZ84_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWU66333.1"
                     /translation="MSLKFRRLLMATLTGSLLTGLTSIAHADSTTDLVQALVSKGVLT
                     EEEGALLMKGRTNEVEVQKKKESKSWTSRVNIRGYVQTRNTTMLGGDEGINLWSDRSV
                     GDDKSLGDADKNFLIRRARVIIFGDYGDHLSYYIQPDFASSASGTNNIAQLRDAYGDV
                     NFDKTKVHRIRVGQSKVPYGFENLQSSSNRLALDRNDALNSAVRDERDLGAFYYYTPT
                     DVQALFEEINKQGLKHSGNYGMFALGLYNGQGANRAEQNDNYHVVSRLTYPWKTQSGQ
                     IYEAGIQAYKGEYVSTTGAYSRLNPATGALASATPTLGTGTPLSGRNGIDDERVGISF
                     IMYPQPFGIQAEWNWGNTPGIDITKNVIEKKSLNGGYVQAMYKIDNFQFLSTNGTLIP
                     FIKWQYFDGYNKAETNSPENHVNDWELGVEWQLAPELELTAVYHRMNRTNLV"
BASE COUNT         1093 a          911 c         1053 g         1063 t
ORIGIN      
        1 ttcacagcct tgcaccgccc cggcaacctg ctcacctcct gttgttgaaa atcatcatga
       61 aacccgctta ggtacttggc gggttttaaa ttacgcacag ctttcacttc attttccgct
      121 gtcggcgctt ttattttgca tcctctccag cgctattttt gccggcatcc tggcctggtc
      181 aatctcgcat ccggtcaaac tcttgcttga tcacctgaaa agtgcggcta aaggcgactt
      241 tctgattggc gtcagtccta aaattacagc tagccatgac gagttctcag agttaggcga
      301 ggcgttggat ctgatgacgc ttcgtattga taatatgatc aaaagccaat ccaacatgct
      361 gcatcatgtt tcccatgagt tacgctctcc acttgccaga atccagatgg ctgtgggatt
      421 ggtcatgcaa aatcccaaaa aaatgcaaag ttcattgcag cgtgttgagc tggaagctgt
      481 tcgcatggag agaatgattt ccgaattgct ggagattttt cgctttgaat ctgggatgca
      541 aaagctgcaa aaaaccgagg tggatttaaa gggtctgctg actgggattg tgggtgatgt
      601 tttatttgaa aagcacgaag cgaagctccg tcttgatatg ccaggagatc ctgtcgtcgt
      661 agaggggcag ctcgagttgt tgcaaagagc catcgataat gtgatccgca atgcagtgaa
      721 atatggacca gaggagggtg tcgtggccat cgagctacaa cacagtgcgc gtactggcga
      781 agtgatttta gaggtgcagg accagggcga aggcgtagaa cctcaagagt tagagcagat
      841 tttcagacca tttgtgcgtg gtcgtcgtgc ctcacaagtt gatggccatg gtattggcct
      901 ggcaatgacc aagcatattg tcgaggctca cggtgggttt gttaaggcgg ctaacttgaa
      961 tccacatggc ttcatgatcc gtatccactt gccagcaccg caaaagcatc atgctcaaga
     1021 gaccaaaaaa tggatgtaaa aatgctaaaa tagcaagttt gagataacgc agacgattgc
     1081 cggatatttt atgacagcac aacacgccaa cccagacagt acaacagaat cagtcgtgga
     1141 tgagaatcac gtgattgcgg aacgtcgcga gaaattacgt ttactgcgcg aacaggcgca
     1201 ttccgcacaa gccgccgtgt tccccaatga tttcaaacca gagcataaag ccgcagcctt
     1261 gcacgccgct tatgccgaag tcgataaaga aacattggat ccgcaagaac tgccagtggt
     1321 ggttgctggc cgtatgatgc tcaagcgtgt catgggtaag gccagctttg caacgatcca
     1381 ggacagttcg gctcgcattc agttatacat tgcacgtgat gaaattggtg aggagctata
     1441 cgaaagtttc aaacattggg acatgggtga tattttgggt gcgcgtggcc gcttgtttaa
     1501 aacgcgtact ggcgaactgt cagtgcatgt gaccggattg cggttgctga ccaagtctct
     1561 gcgtcccttg cctgagaagt ttcatggcct gcaggatcag gagatcaagt atcgccagcg
     1621 ctacgttgat ttgatcactt ccgaagaaac ccgaaccacg tttaaggcac gctctaaagt
     1681 ggtcgctgca attcgtcaat tcatgaacga caacgagttt ctggaagttg aaacgccgat
     1741 gttgcacccg attccaggtg gcgcttcggc caagcctttt atcacacatc ataatgccct
     1801 ggatatgcag atgtttatgc gtatcgcacc ggagctttat ttaaagcgcc tgattgtggg
     1861 tggctttgag cgcgtgtttg aaatcaaccg taacttccgt aacgaaggcc tgagtgtccg
     1921 ccataaccct gaattcacca tgatggaatt ctatgcggca tacactaact atcagtggtt
     1981 gatggatttt accgagcaat gtattcgcgc agcagccatt gctgcaaatg ggaccgcagt
     2041 gcttagctac gccggcaaag aggtggattt gagcaagccg tttgagcgct tgacaattat
     2101 tggtgcgatt cagaagtatg caccacaata tacgctggat caactcaatg atgtcgattt
     2161 cttgcgcaag gaattgctga agtttggtgt aaaaccattt gatcatgccg gcctgggtgc
     2221 attgcagttg tcactgtttg aagagacggc tgaaagccaa ctttggcagc cgacttacat
     2281 tattgactat ccggtagagg tgtcaccact ggcacgtgct tcggataagc atccggaaat
     2341 taccgagcgt ttcgagttgt ttatcactgg ccgtgaaatg gcaaacggtt ttagcgagtt
     2401 gaatgatgct gaagaccagg ctgcccgctt ccatgcacag atgaaagcca aggaagccgg
     2461 tgaccaggaa gcgatgtact atgatgccga ttttatccgt gcactcgaat acggcatgcc
     2521 gccgacaggt ggttgcggga ttggcataga ccgcttggta atgttaatta cggatagtcc
     2581 aagcatccgc gatgtgattc tattcccgca tatgcgcgcc gaaagttaaa cctgctagat
     2641 atagcatata gccaccctct ttgggtggct tttttatgac aaatagtctg cccgtaagga
     2701 aactgtaagg tttgtcatca aactgtcata ttgaaaactc aaaatacgcc ccggatacac
     2761 acagtaatct tataacaggg gataaaaatg agtttgaagt ttcgtcgttt gttgatggca
     2821 accttgacgg gtagcctgtt aacagggttg acgtctatcg cacatgctga ttcaacgact
     2881 gacttggttc aggcattggt tagcaaaggc gttttgactg aagaagaggg cgctttgctg
     2941 atgaagggcc gtacgaatga agttgaagtg cagaaaaaaa aggaaagtaa aagctggacg
     3001 agtcgtgtga atatacgcgg ttatgttcaa acaagaaata cgaccatgct gggtggcgat
     3061 gaaggcatta acttgtggtc agatcgttca gtcggtgacg ataaatcctt gggtgatgca
     3121 gacaagaact tcctgatccg ccgtgcacgc gtcattattt ttggcgatta cggtgaccac
     3181 ctgagttatt acatccagcc agactttgcg tcatcagcca gtggtactaa caatattgcc
     3241 caattgcgcg acgcttatgg tgatgtcaat tttgataaaa ctaaagtaca tcgtatccgt
     3301 gttggtcagt ctaaagttcc gtatggtttc gaaaacctgc aatcctcttc aaaccgcctg
     3361 gcgttggatc gtaacgatgc attgaatagt gctgtcaggg atgagcgcga tctgggtgct
     3421 ttctactact acacgcccac agatgttcag gcgttgtttg aggaaatcaa caagcagggc
     3481 ctgaaacact ctggcaacta cggtatgttt gcacttggtt tgtacaatgg ccagggcgcc
     3541 aaccgcgctg agcagaatga caattatcat gttgtatcgc gcctgactta tccatggaaa
     3601 acacaaagtg gccagattta tgaagccggt attcaagctt ataaaggcga atatgtttca
     3661 acaacaggtg cttatagccg ccttaatcct gcgacaggcg ctttagcttc tgcaaccccc
     3721 actttaggta caggtactcc tctttctggc agaaacggta ttgatgatga gcgtgtaggt
     3781 attagcttta tcatgtatcc acagccattt ggtattcagg ctgaatggaa ctggggtaat
     3841 acaccaggta ttgatatcac caagaacgtg attgaaaaga aaagcctgaa tggcggatat
     3901 gtacaagcca tgtataaaat cgacaacttc cagttcctga gcacgaatgg cacattgatt
     3961 ccatttatca aatggcaata ctttgacggt tataacaaag cggaaaccaa ctcgccagaa
     4021 aaccacgtca atgactggga gttgggtgtg gagtggcaac tggcgccaga gcttgagttg
     4081 acggcggttt accatcgtat gaatcgtact aacctggtca
//
LOCUS       DATMSK010000145         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_127982,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000145 DATMSK010000000
VERSION     DATMSK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127982"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2352
                     /gene="glnE"
                     /locus_tag="VNZ84_04940"
     CDS             <1..2352
                     /gene="glnE"
                     /locus_tag="VNZ84_04940"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HWU66334.1"
                     /translation="QLAITYGLPRDASGTVQQLIVVGMGKLGGRELNVSSDIDLIFAY
                     EQEGVTDGEKSISNQDFFTRLGKKVIGALDEVTADGFVFRVDMRLRPFGSEGALVCNL
                     DALEDYYQNYGREWERYAWIKGRAVYGPGEALAKVMKPFVFRKYLDFNAYASMRDLKV
                     QIQRDVVQRRLSNNIKLGRGGIREVEFIAQVFQLIRGGQVPDLQIKPTLAVLDCLVER
                     GMLPAADVAALKESYIYLRNLEHRLMYIDDQQTQDLPDSETYRGYLLQMVKHADWASF
                     MTELNTHRDRVQRCFDDTFKDEQSSDLQQETALWQGTLSTAEGEQWLVQHGYTRAGDI
                     VHLLQQTRRSQRIQQLPEQSRRRLDQLMPLLLREASQQQSTTPDTALIRTLDLLESIC
                     RRASYLALLAEFPDALALIVKLCAASPWIAQYVSAHPILLDELLNKQTLLKRLDAAQL
                     KAELTRHMEQLAGDVEAQMDAMRHFKHANVLKIAAQDLMTALPLEAISDDLSALADVI
                     LQVSVQTVWQNLSFKHLDTPKFAVIGYGKLGGKELGYVSDLDIIFLYDDEAEKAQEIY
                     ARFAQRLNTWFNTLTNAGLLYETDLQLRPDGNSGLLVSTVKAFADYQQHKAWVWEHQA
                     ITRARFVAGDSAIGAAFERIRHDIMCQPRVIEALSQDIVQMREKMREAHKPKPGFFDV
                     KQGLGGIIDVEFIVQYLVLAHAASHPQLTDNIGNIGLLRRCVQLGLIESSTLAEQVVN
                     AYREYRTLIHHVKLQGAEAIVPIDELQAERESVRRLWQVLFPE"
     gene            complement(2361..>3658)
                     /locus_tag="VNZ84_04945"
     CDS             complement(2361..>3658)
                     /locus_tag="VNZ84_04945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWU66335.1"
                     /translation="DPTALEWMVSLETPIDITPTNTLNIGHDILLNALFNRVFNDHLS
                     GTYNFIFRQDGRLIAHPAKIEDMNRTRGMLMISQLKDAQLSHYFQALQARIEKKQSNY
                     FITETEDEFLAATKIEGPGWWFVTVYPKSLLSSPSLNAARFILIISILALIIELVMLY
                     LVMKKKVVEPLEAFIAASSAIEAQRYDKITGNVLPLPDQLDNEIGKLAKLFKSMSSSL
                     QQFRGDMQHLTHTLEDQVKVRTRELQKAKDKAEKEATIDSLTQIPNRHSFYNRAKALL
                     QQADHTGDTVCFLMLDIDFFKIINDSYGHPQGDQVLIACARTIEKTIRDKDLLGRLGG
                     EEFAVVLPGTNLEEAQVIAERIRARIAEQAFHTTQHTQSFHTTISIGISSSSETIYAY
                     ELLYKHADLALYKAKQSGRNRVVSYSGTWPTTPEDAPAT"
BASE COUNT          858 a          812 c         1035 g          953 t
ORIGIN      
        1 caattggcaa tcacctacgg tttaccacgc gatgccagtg ggacagttca gcaactgata
       61 gtggttggta tgggtaagtt aggtgggcgt gaactgaatg tatcgtcgga tatcgacctc
      121 atttttgcct atgagcaaga aggtgtcaca gatggcgaga aatcaattag caatcaagat
      181 tttttcacgc ggttaggcaa aaaagtcatt ggggcactgg atgaagtgac tgccgatggg
      241 tttgtattca gggtagatat gcgtttacgc ccgtttgggt ctgagggtgc actggtatgt
      301 aacctcgatg cgctggaaga ttattaccag aattatggcc gtgagtggga gcgctatgcc
      361 tggattaaag gccgtgctgt atatggtccg ggtgaagcgt tggctaaagt gatgaagcca
      421 tttgtgttcc gcaagtacct ggattttaat gcctatgcct caatgcgcga cctgaaagtc
      481 cagattcagc gtgacgtggt gcaacgccga ttatccaata atattaaact ggggcgcggt
      541 gggatccgtg aagtagagtt cattgcgcag gtattccagc tgatccgtgg cggccaggtg
      601 cctgatctgc agatcaagcc gacgttggct gtactggatt gcctggtgga gcgaggtatg
      661 ttgccggctg cagatgtggc ggccttgaaa gaaagttata tctacctgcg taatttggaa
      721 caccggttga tgtatatcga tgatcagcaa acgcaggatt tgcctgactc tgaaacctat
      781 cgcggttatt tgttgcagat ggtgaaacac gctgactggg cgagttttat gaccgagctt
      841 aatacgcatc gcgaccgtgt acagcgttgt tttgatgaca cctttaaaga cgaacaaagt
      901 agtgatttgc agcaggaaac tgcgttgtgg cagggcacat tgagcacggc ggaaggagag
      961 cagtggttag tacagcatgg gtatacgcgc gccggtgata ttgtgcattt gctgcagcag
     1021 acccgtcgca gccagcgtat acagcaactg cctgagcaaa gccgccgccg cctggaccag
     1081 ttgatgccct tattactgcg agaggccagc cagcagcaaa gtactacgcc agatactgcg
     1141 ctgatccgga cgctggattt gctggaaagc atttgccgcc gtgccagtta tttagccttg
     1201 ctggccgagt ttccggatgc actggcgtta attgtcaaat tgtgtgcagc cagtccctgg
     1261 atcgcgcaat atgtttccgc acacccgatc ttgctggatg agttgctgaa caagcaaacc
     1321 ttgcttaaac gtcttgatgc cgcgcagttg aaagcggagt taacgcgcca tatggagcaa
     1381 ctggcggggg atgtcgaagc acaaatggat gccatgcgac atttcaagca tgccaatgta
     1441 ctcaagattg ccgcgcagga cttgatgacc gcattaccgc tggaggcgat ttctgatgat
     1501 ttaagcgcgt tggcagatgt gatcctgcaa gtgagtgtgc aaactgtttg gcaaaacctc
     1561 tcgttcaagc acctagatac gccaaaattt gccgtgatag gctatgggaa gctggggggc
     1621 aaggagctgg gttatgtatc cgacctagat attattttcc tgtatgacga tgaggctgaa
     1681 aaagcgcagg aaatttatgc gcgctttgcc cagcgcctga atacatggtt taacaccctg
     1741 accaatgcag gactgttata tgaaacggat ttgcaattgc ggccagatgg caacagtggg
     1801 ttgctggtta gcacggtgaa agcgtttgca gactaccagc agcacaaagc ctgggtatgg
     1861 gagcaccagg ccatcacccg ggcgcgtttt gtcgcaggag atagtgcgat aggtgctgca
     1921 tttgaacgca tccgacatga catcatgtgc cagccacgcg tgatagaggc tttgagccag
     1981 gacatcgttc aaatgcgcga aaaaatgcgc gaggcgcata aacccaagcc aggctttttt
     2041 gatgtgaagc aggggctggg tggcattatt gatgtcgagt tcattgtgca atacctggtg
     2101 ctggcgcacg cagccagcca tccgcaactg accgataata tcggtaatat cggtttgttg
     2161 cgacggtgtg tgcagttagg attgattgag tcatctacgt tggctgaaca ggtggtcaac
     2221 gcctaccgcg agtatcgtac cttgatccat catgtgaagt tgcagggcgc agaggcgatc
     2281 gttcctattg atgagcttca ggctgagcgt gagtcagtca ggcgtttgtg gcaggtgctc
     2341 tttcctgaat aattaactgc ttaggtggca ggagcatctt ccggagttgt aggccaggtg
     2401 cctgagtaac tgaccacacg gttacggccg gattgtttgg ctttgtacag ggccaggtcg
     2461 gcgtgcttgt agagcagctc ataagcgtaa atagtctctg atgagctcga aatgcctatg
     2521 ctgatagtgg tatggaaact ctgtgtatgc tgtgtcgtgt ggaatgcttg ctctgcaatc
     2581 ctggcccgta tccgttcggc aatcacttgc gcttcttcga ggttggtgcc gggcaaaacc
     2641 actgcgaatt cttcaccgcc caacctaccc agcaggtctt tgtcacggat ggttttttcg
     2701 atggtgcgtg cgcaggcaat caagacctga tcaccttgcg ggtgaccgta agagtcgttg
     2761 ataattttaa agaaatcaat atccagcatc aggaagcata cagtgtcacc agtatggtcc
     2821 gcctgctgca ataaggcttt ggcgcggttg taaaatgaat ggcggttggg aatttgtgtc
     2881 aggctgtcga tggttgcttc tttttctgct ttgtcttttg ccttttgtaa ttctcgcgtc
     2941 ctaaccttaa cctggtcttc gagcgtgtgc gtgagatgct gcatatctcc gcggaattgc
     3001 tgcaggctgc tggacatgct tttaaacagt ttggccagtt taccgatttc gttatccagt
     3061 tgatctggca gcggtagcac attgcctgtg attttgtcgt aacgctgggc ctcaattgcg
     3121 ctagatgccg caataaaagc ttccaatggc tccacgactt tcttcttcat gaccaggtat
     3181 aacatgacaa gctcaataat cagggccaat atactgatga tcaggataaa cctggctgca
     3241 ttgagggaag gggatgacag cagggacttg gggtaaacgg tgacgaacca ccatccaggg
     3301 ccttcaattt tggtcgccgc caggaattcg tcctctgtct cggtgataaa gtaattcgac
     3361 tgcttcttct caatcctcgc ctgcaaagcc tggaaatagt ggctgagttg agcatctttt
     3421 aattgcgaaa tcatcaacat gccgcgagtg cggttcatgt cttctatttt tgccgggtgt
     3481 gcaatcaggc ggccatcttg cctgaaaatg aagttgtagg taccagacaa gtggtcatta
     3541 aagacacgat taaacaaggc attcagtaag atgtcatgac cgatattcaa ggtgttcgtt
     3601 ggcgtaatgt cgatcggtgt ttccagggac accatccact ccagtgcagt cgggtcgt
//
LOCUS       DATMSK010000146         9437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_457368,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000146 DATMSK010000000
VERSION     DATMSK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457368"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..998
                     /locus_tag="VNZ84_04950"
     CDS             93..998
                     /locus_tag="VNZ84_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HWU66336.1"
                     /translation="MTLIELKFVLAVAQERNFRRAAEKCFVTQPALSLGIKKLEEELG
                     VLLFERNRNEVTPTEIGEQIIAQANVVLEQAAKIKDMAKHGNDPLNGLFKLGMIHSVG
                     PYLLPEIIPPLIKTAPHMPLEVEENITATLEQQLKNGVIDAAVIALPFNVPGIETTPL
                     YDEDFNVVVPVNHPWATRKSIHAEELSTEKVLLLNTGHCFSNQVTQACPELSRKGEVL
                     QGNSLETIRNMVASNLGITVLPAMATAARYQNPLIRVIPFSKPAPSRRIALAWRKSFV
                     RTAAIEVLGKVMAEVGEGKAQISRL"
     gene            complement(1009..1896)
                     /locus_tag="VNZ84_04955"
     CDS             complement(1009..1896)
                     /locus_tag="VNZ84_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HWU66337.1"
                     /translation="MSLITACPACQTQFEVTDEQLQAYAGKVRCGECNHVFDARSFLL
                     EPSVSPVAVPGAGESEEAASDAAVYFKVSAEAAQVEHSPLLPDQSLQPDLALEQTGSP
                     TWNINLDDGVDVEPSIPDFLRDVSLADERPAPVQSVASQRVFASLSGILLVAILLQVV
                     YFTRTNLAANYPQTKPWLLAMCQRLHCELNLPHDITQLTIDDADIQEHRERQGVLAFS
                     SVLMNHGTVVQAYPKVELTLTNTADEAVLRKVLQPAQYLPPSVDHNVGLAPQQEQAVR
                     VFLGVDEKLVTGFRVAIAY"
     gene            complement(1887..2789)
                     /gene="prmA"
                     /locus_tag="VNZ84_04960"
     CDS             complement(1887..2789)
                     /gene="prmA"
                     /locus_tag="VNZ84_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWU66338.1"
                     /translation="MHWLLLKIQSTEPQAEALSDVLMEHGVLSVSIEDAHADTLAEQA
                     IFGEPGDPPPGIWQQNIVTAMLDAEADVQALLANVQSELELEGLKYQVEQIEEQDWVR
                     ATQAQFDPIRVTDDLWIVPSWHEAPNAEAINIVLDPGLAFGTGSHPTTHLCLSWLAQL
                     PKALLAQSHVLDYGCGSGILAIAAKKLGAKSATGVDIDPQAVIASRDNAVNNQVDAIF
                     YDSADYLHEPFDIVVANILSSALMVLAPVIANSCKSGGKVALSGILDSQQEMLLAHYS
                     EWFEMDAPIQQDGWVLLTGTRKCP"
     gene            complement(2792..4141)
                     /gene="accC"
                     /locus_tag="VNZ84_04965"
     CDS             complement(2792..4141)
                     /gene="accC"
                     /locus_tag="VNZ84_04965"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWU66339.1"
                     /translation="MFEKILIANRGEIALRVQRACRELGIKTVAVHSEADRDAKYVKL
                     ADESVCIGPAPSAKSYLNIPAVISAAEVTDAQAIHPGYGFLSENADFAERVEQSGFVF
                     IGPRAETIRLMGDKVSAKDAMKKAGVPCVPGSDGALTDDPAETIKTAKRIGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVNMTKAEAQSAFGNPMVYMEKFLENPRHIEIQILADQ
                     HGNAVFLGDRDCSMQRRHQKIIEEAPSPLLPARLRDKIGERCAEACRKIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHTVTEMITGIDLVQQQIFIAAGEKLPFRQRDIVLNGHA
                     IECRINAEDPYNFVPSPGFINRFHMPGGPGVRMDTHVYGGYTVPPHYDSMIGKLITHG
                     ATREQAIARMRVALSEMYVEGIKTNTPLHVDLMADLAFQQGGTSIHYLEQKLGISSK"
     gene            complement(4148..4594)
                     /gene="accB"
                     /locus_tag="VNZ84_04970"
     CDS             complement(4148..4594)
                     /gene="accB"
                     /locus_tag="VNZ84_04970"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HWU66340.1"
                     /translation="MDLRKLKKLIDLVEESGISELELTEGEEKVRISRAMAPGAAPVT
                     QYVAAPAPAPVAPAAAAPAAAPAEALDGKVVKSPMVGTFYRASSPDAKAFVDVGSSVN
                     TGDTLCIIEAMKLLNEIESEFSGTIKKIFVENGQPVEYGEPLFLIG"
     gene            complement(4629..5066)
                     /gene="aroQ"
                     /locus_tag="VNZ84_04975"
     CDS             complement(4629..5066)
                     /gene="aroQ"
                     /locus_tag="VNZ84_04975"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWU66341.1"
                     /translation="MGAKSILVIHGPNLNMLGTREPQHYGSQTLADINQRLQELARAD
                     QVRLETFQSNAEADIIEKIHALSVNKVDYIIINPAAFTHTSIAIRDAISAVKVPFIEV
                     HLSNVHARESFRHHSYFSDIATAVICGLGAEGYFAAYRYIQQL"
     gene            complement(5261..5794)
                     /locus_tag="VNZ84_04980"
     CDS             complement(5261..5794)
                     /locus_tag="VNZ84_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWU66342.1"
                     /translation="MRNVARIVMTVLLIVVLGSGFRWLYLNRHQFMATESSLPGETEV
                     AALWASTLPDHTGKDYPLAQFKGKPLILNFWATWCEPCREEMPEISALASAHPEIAVL
                     GLAIDEAAAVHEFVESTPVSYPLLIAENDGMPLAETLGNHKGVLPYTVIISAQGVVTQ
                     IFFGRVNREMLQKALNL"
     gene            complement(5787..7580)
                     /gene="dsbD"
                     /locus_tag="VNZ84_04985"
     CDS             complement(5787..7580)
                     /gene="dsbD"
                     /locus_tag="VNZ84_04985"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HWU66343.1"
                     /translation="MLAMMWLSVLPAQAESQFSKLFTDSDSEEFLPVDQAFKVEAGLV
                     DANHAQLKFIVAPGHYLYRDRIQLVSPVDGTANIALPAGETKDDPNFGEQQVFHHDTL
                     AEVTFSGTAPATIKVRYQGCSEKGLCYPPQTKSLELGAVTSSSTTNNLAIADQETSVQ
                     AEEEFSGKLLASGHWWQIVAGFFGAGLLLAFTPCVFPMIPILSGIIVGKNAHVSRGKA
                     FTLSLAYTLGMCLTYTLAGVAAGLSGQLLSNALQTPAALIAGALIFVVLSFSMFGFYE
                     LKLPSAVENYFFNWSNRFKGGHLFSDFLMGAISALVISPCVAAPLAGALLYISQTHDV
                     TLGAVALFSLSLGMGVPLLLIGASAGTILPKAGEWMNTIRRLFGVLMLGVAIWIVSPI
                     LPVALQFILWAAICIVPAIYLRAIDPLPATATTLQRLFKGFALMLLLYGVALLIGAWS
                     GARSPLQPLQNLTTGKSANMQPLAFQRVHNLQELQQRVQTAGQPVMLDFYADWCVSCK
                     EYEQFVFSDPAVRSELKGVLLLQADVTDNNPEDAALLKRFELFGPPGIVFFNAQGQAL
                     SPVIKGYQDAERFARSVRALKLSSTEASPHA"
     gene            complement(7615..7950)
                     /gene="cutA"
                     /locus_tag="VNZ84_04990"
     CDS             complement(7615..7950)
                     /gene="cutA"
                     /locus_tag="VNZ84_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HWU66344.1"
                     /translation="MTPKEEIIIVFTHVPNRACAEHIAQTLVTEKLAACVNISSPVTS
                     IYQWQGKVERAEEVGLTIKTSCKGYAACETKLRMLHPYELPEIVGIHVNEGLPEYLAW
                     VTAETLLQE"
     gene            7980..8435
                     /locus_tag="VNZ84_04995"
     CDS             7980..8435
                     /locus_tag="VNZ84_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HWU66345.1"
                     /translation="MRFFIFAILLAFPFLEIAVLIELCQRYGWWVLVYLVIIGYLGLK
                     LIRSEKDLIAARMFQQMSAGGNPVSALLSTARNMFAGVLLLIPGVISDALAVILLLIP
                     TKNPGLGKSGQNNGTYYQTSANDEVIEGEFTEVKETKAADNVVHLPRKD"
     gene            8457..>9437
                     /locus_tag="VNZ84_05000"
     CDS             8457..>9437
                     /locus_tag="VNZ84_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HWU66346.1"
                     /translation="MNRVLIENSGHTFDVRPSQTVLEAAIEAGINMPYGCRNGACGAC
                     KGKILTGKVMHDDYQGNALTDAELAAGQALFCCARPLEDLVIECRQIDMVQGIKPRIL
                     PVRVQNKEQFGDDVMVLHLQLPGTEQLSFMAGQYVEFLLKDGRRRAFSIANAPHEKGF
                     LELHIRKIAGGHFTEQVFNEMPVKTILRIEAPLGSFFLREQNEKPILMVAGGTGFAPV
                     KGMIEHMIFNQIQRPVYLYRGARQVGDLYMDELCQRWAQFMPNIQYIPVISDGKEGDG
                     WDGRRGLVHEAVLEDHADLSGFEAYVCGAPGMVDISKETFVKQGLPEEEFY"
BASE COUNT         2502 a         2461 c         2233 g         2241 t
ORIGIN      
        1 agccgcttca atttgtacaa ttgtatgttt tgatattatt gattaataaa attaatggca
       61 ttacacttta ataaatacct ttagagatca ccatgaccct gattgaattg aagttcgtgc
      121 tagccgtcgc gcaagagcgc aattttcgcc gtgcggcaga aaaatgtttt gtgacacaac
      181 ccgcattaag cttgggcatc aaaaagctcg aagaggaatt aggcgtgttg ttgtttgaac
      241 gcaaccgtaa tgaagtgacc cctactgaaa tcggcgagca aattattgcg caggcgaatg
      301 tggtattgga gcaagcagca aaaattaaag atatggccaa acacggcaac gatccgctta
      361 acggcctgtt taagctaggt atgattcatt cagtgggccc ttacttgctg ccagagatca
      421 ttccaccatt aatcaagacc gcaccgcaca tgccactgga agttgaagaa aacatcaccg
      481 ccacactgga gcagcagctc aaaaatgggg tgattgatgc cgcggtgatt gctttgcctt
      541 ttaatgttcc aggtattgaa accacgccgc tttatgatga ggactttaac gtggtggtgc
      601 ctgtgaacca cccttgggcc acgcgtaaaa gcatacatgc cgaagaatta agcactgaaa
      661 aagtattgtt gttgaatact ggccattgct ttagcaatca ggttacgcag gcctgtccag
      721 aactgagccg caaaggcgaa gtattacaag gaaactctct ggaaaccata cggaatatgg
      781 tcgcttcaaa cctgggcatc acggtattgc ctgccatggc gactgctgca cgctaccaga
      841 atccgctcat acgcgtgatt cccttcagca aacctgcccc tagtcggcgc attgcactgg
      901 cctggcggaa aagttttgtg cgtacagcag cgattgaggt acttggaaaa gtaatggcgg
      961 aagtaggaga gggaaaggcg cagatttcac gcctttgaaa aatagcgatt agtaggcgat
     1021 ggcgacgcga aaacctgtga ccagcttttc atccacgccc agaaaaactc ttaccgcctg
     1081 ctcctgctgg ggcgctaacc caacattatg atcaacggat ggcggcaagt attgtgctgg
     1141 ctgcaacact ttgcgcaata ccgcttcatc agccgtattg gtcaaagtga gttcaacttt
     1201 gggataagct tgtacaacgg tgccgtgatt catgagcaca ctggaaaaag ccaatacgcc
     1261 ctggcgctcc ctgtgttcct gaatatcagc atcatcaatc gtgagctggg taatatcgtg
     1321 cggtaaattc aactcacaat gcaagcgttg gcacatagcc aacaaccatg gcttggtttg
     1381 cggataattt gcagcgaggt tagtacgtgt gaagtagact acttgcagca aaattgccac
     1441 tagcaggata cctgacaacg atgcaaaaac ccgctgactg gcaacacttt gcacaggggc
     1501 tggccgctca tctgccagcg atacatcccg cagaaaatcc gggatcgaag gctcaacatc
     1561 cacaccatca tcgagattga tgttccaggt tggcgagccc gtctgctcaa gcgccagatc
     1621 gggctgtaat gactggtcag gcaggagcgg tgaatgctcc acctgcgccg cctcagcgct
     1681 tactttaaaa tatacggcgg catcactggc cgcttcttcg ctctcaccag ccccaggtac
     1741 agccactggc gatactgatg gctccagcaa aaacgagcgc gcatcaaaca catgattaca
     1801 ttcaccacaa cgcaccttgc ctgcatatgc ttgcaattgt tcgtcggtga cttcaaattg
     1861 tgtttggcag gccgggcagg ccgtgattaa ggacattttc tggttcccgt cagcaagacc
     1921 catccatctt gctggatggg ggcatccatt tcaaaccact ctgagtaatg tgccagcaac
     1981 atctcctgct ggctgtctaa tatgccggat aatgcaactt taccgccact tttgcaagaa
     2041 ttggcaatta cgggggccaa taccatcaag gcactggaca gaatgttggc aaccacaata
     2101 tcaaacggct cgtgcaagta gtccgcggag tcataaaaaa tcgcatctac ctgattgttc
     2161 accgcattat cacggctggc aatgaccgct tgtggatcaa tatccacgcc tgtcgcactt
     2221 ttagcaccca attttttggc tgcgatcgcc aaaatacctg acccacaacc ataatcgagc
     2281 acatgcgact gcgccagcaa agctttaggt aattgcgcaa gccaacttaa gcataaatgc
     2341 gtggttggat ggctgcctgt gccaaatgcc aaccctggat cgaggacgat attaatcgcc
     2401 tcagcattcg gcgcctcatg ccaagaaggc acaatccaca aatcatccgt cacacgaatg
     2461 gggtcgaact gggcttgtgt tgcacgcacc cagtcctgct cctcaatctg ctcaacctga
     2521 tatttcagcc cctctaactc aagctcagat tgcacattcg caagtaatgc ctgtacgtca
     2581 gcctccgcat ccagcatggc cgtgacaata ttctgttgcc agattcctgg tggcggatcg
     2641 ccaggctcac caaaaatggc ttgttcggcc agggtgtccg cgtgtgcatc ttcgatgctc
     2701 actgacaaca caccatgctc catgagcacg tcactgagcg cctcggcctg aggttcggtc
     2761 gattgaattt taagtaacag ccaatgcaaa attatttaga gctgataccc agcttctgct
     2821 ccaaatagtg aatgctggtg ccaccctgct gaaaagccag gtcggccatc aggtcaacat
     2881 gcaacggtgt attggtttta atgccttcca cgtacatctc ggacagtgct acacgcattc
     2941 tggcaattgc ctgctcacgg gttgcaccat gcgtgatcag cttgccgatc atggagtcat
     3001 agtgcggagg cacagtgtaa ccgccgtata catgggtatc catacgtaca cctgggccac
     3061 ccggcatatg aaagcggttg ataaaacctg gagagggaac aaagttatac ggatcctcag
     3121 cattgatgcg gcattcaatc gcatggccat tcagtacgat atcacgctgg cggaacggca
     3181 gtttttcgcc tgcagcaatg aaaatctgct gttgtaccag gtcaatcccg gtaatcattt
     3241 cagtgaccgt atgttctacc tgcaaccgcg tattcatttc aatgaaataa aactcgccat
     3301 tttcgtacag gaattcgaaa gtacccgcac cacggtaacc aatcttgcgg caggcctctg
     3361 cacaacgttc accgattttg tcgcgcaatc ttgctggcaa caaaggggag ggggcttctt
     3421 caataatttt ctggtggcgg cgctgcatgg agcaatcacg gtcacccagg aatacagcgt
     3481 tgccatgctg gtcagccaga atctggatct caatatggcg cggattttcc agaaattttt
     3541 ccatgtaaac catggggtta ccaaacgcac tctgcgcttc ggctttagtc atattcaccg
     3601 cattgagcaa tgcagcctct gtatgcacca cacgcatgcc acggccaccg ccgccacctg
     3661 ccgctttaat aatgacaggg tagccaatac gcttggctgt cttgatggtt tcagctggat
     3721 catccgtcag tgcaccgtct gaacctggca cacaaggcac gcccgctttc ttcatggcgt
     3781 ctttggccga caccttatcg cccattaggc ggatggtttc agcacgcggc ccaataaaaa
     3841 caaaaccgct ttgctcgaca cgttcggcaa aatcggcatt ttcagacaga aaaccatatc
     3901 ctggatgaat ggcctgtgca tcagttacct cagcagcact aatcaccgcc ggaatgttca
     3961 agtaactttt agcagaaggg gcggggccaa tgcatacgga ctcatccgca agcttgacgt
     4021 atttggcatc acggtcagct tcggaatgga cagcgaccgt tttaatcccc aattcgcggc
     4081 acgcacgttg cacgcgcagg gctatttcgc cgcggttggc aatcagaatt ttctcaaaca
     4141 tagtggttta accaatcagg aacaggggct cgccgtattc gacaggctga ccgttttcaa
     4201 caaaaatctt tttaatcgtg ccagaaaact cgctctcaat ttcattaagc agcttcattg
     4261 cttcgataat acacagagta tcaccggtat tcacgctgct gccgacatca acgaaagctt
     4321 tagcatcagg tgaagacgca cggtagaacg tacccaccat tggtgatttc acaactttgc
     4381 catccagcgc ttcagccggt gctgcggctg gcgcagctgc cgctggagcc acaggagccg
     4441 gagcaggggc ggcgacatat tgcgtcaccg gtgcggcacc tggcgccatg gcacgactga
     4501 tacgtacttt ttcctcaccc tcggtcagtt ccagttcgga aatccccgat tcctctacca
     4561 gatcaatcag ttttttgagt ttgcgtaaat ccataaaagc ctcgaatgtg taaatgtttt
     4621 aagtgttatt agagttgttg gatgtagcga tatgcggcga agtatccctc tgcgcccaaa
     4681 ccgcaaatca cggcggtagc aatatcgctg aaataagaat gatgccggaa tgactctcgt
     4741 gcatgcacat tggagagatg cacttcaata aacggcacct tgactgcaga aatggcatcg
     4801 cgaatcgcga tagaagtatg tgtgaacgcc gcaggattaa taatgatgta atccaccttg
     4861 ttcacggaca atgcatgtat tttctcgatg atgtcagctt cagcgttgct ttgaaatgtt
     4921 tccaaacgca cctgatcagc ccgtgccaac tcctgcagac gttggttgat gtctgcaagc
     4981 gtttgactac cataatgctg cggctcacgt gtgcccagca tatttaaatt gggtccgtga
     5041 atcaccagga tggatttggc gcccatttca catgcctctt atggagaaaa atacaacctt
     5101 catggcgtcg aagtttgccg aagttattgc attttgtcca gcagaattcg cagtttaaag
     5161 aaaattgaaa gtcgctaatt tggccgacaa ttgtagaggt ttggtcttga acaagcagga
     5221 tctggtaggg tagaggtgac ggttaaaaac tatttagccg ctataaattc agcgcttttt
     5281 gcagcatttc acggtttacc cgcccgaaaa atatttgtgt gacaacgcct tgtgccgaaa
     5341 tgatgaccgt ataaggcaac acccccttat gattacccaa cgtctcggcc agtggcatgc
     5401 catcattctc ggcaatcagc agtggataac tcaccggcgt gctttctaca aactcgtgta
     5461 cggcagcggc ctcatcaatc gccagcccca gcactgcaat ctctgggtga gcgctggcca
     5521 gcgctgagat ttccggcatt tcttctcgac aaggctcaca ccaggtcgcc caaaaattca
     5581 ggattagcgg cttgccctta aattgtgcca gcggataatc tttgccagta tgatccggca
     5641 atgtgcttgc ccatagcgca gccacttcag tctcaccagg caatgatgat tccgtggcca
     5701 taaactggtg acgattcaaa tataaccagc ggaagccgct accaagcacc acgatcaata
     5761 ataccgtcat gacaatacgg gcaacattac gcatggggtg atgcctccgt agaacttaat
     5821 ttaagtgccc gcacactgcg cgcaaaacgt tcagcatcct gatagccttt aatcacaggg
     5881 gatagcgcct ggccttgtgc attaaaaaag acgatcccag gtgggccaaa caattcaaat
     5941 cgcttcagta aggcggcatc ttctggattg ttatcagtca catcagcctg taacaacagc
     6001 acacctttca attcagagcg cacagcagga tcactaaata cgaactgctc atactcttta
     6061 caagaaacac accaatcggc ataaaaatcc agcatcactg gttgaccggc ggtttgtaca
     6121 cgttgctgaa gttcctgcaa gttatgtacc cgctggaaag ccaatggctg catgttggcc
     6181 gacttgcctg tcgtcaggtt ttgcagcggc tgcaagggag agcgagcccc agaccaggca
     6241 ccaatcaaca aagcgacacc atacagcaaa agcatgagtg cgaaaccctt aaacaagcgc
     6301 tgtagagtag ttgcggttgc aggtaatgga tcaatggctc gcaaataaat ggcaggtaca
     6361 atacaaattg ccgcccacaa tatgaattgc aatgcgacgg gtaagatcgg gctaacaatc
     6421 cagatggcta cccccaacat gagcacacca aacaggcggc gaatcgtatt catccactca
     6481 ccagctttag gcaaaatggt tcctgccgat gcgccaatca ataacagtgg cacacccatc
     6541 cccagcgaca gggaaaacaa tgctaccgcc cccaaggtca catcatgggt ttggctgata
     6601 tatagcaacg cgccggccaa tggcgctgca acacaaggac tgattactaa cgccgaaatc
     6661 gcccccatca gaaagtcgct gaacaaatgc ccgcccttaa agcggttgct ccagttaaaa
     6721 aagtaattct caacagcgct tggcaacttc aactcataaa agccaaacat ggagaaagag
     6781 agcaccacaa aaatcagggc gccagcaatt aatgctgctg gcgtttgcaa ggcattactc
     6841 agcagttgcc cggacaatcc agctgcaacg ccagccagcg tgtaagtcag acacatgccc
     6901 aaggtataag ccaatgacag cgtaaatgct ttgccacgcg acacatgcgc atttttacca
     6961 acaataatgc cagacaaaat cgggatcatc ggaaacacac aaggcgtaaa tgccaacagc
     7021 aggccagcac caaagaagcc cgccacaatc tgccaccaat ggccggaggc cagtaactta
     7081 ccggaaaact cttcttcagc ctgaacgctg gtctcttgat cagcaatcgc caggttattc
     7141 gtcgttgaac ttgaagtgac agcacccaat tccaacgact tggtttgcgg cggatagcac
     7201 aagccttttt cactacagcc ctgataacgt acctttatcg tagcaggcgc cgtgccacta
     7261 aaagtcacct ccgctagggt gtcatgatga aacacttgct gttcaccaaa gttaggatca
     7321 tctttggttt caccggcagg cagcgcgatg ttagcggtgc catccactgg tgaaaccagc
     7381 tgtatgcgat cacggtagag ataatgacct ggcgcaacaa taaatttaag ttgtgcatga
     7441 ttggcatcca ccaatccggc ctctacttta aatgcctgat caactggcaa aaactcttct
     7501 gaatcactgt cagtgaacag cttggagaat tgcgactccg cctgtgcggg caacacgctc
     7561 agccacatca tcgccagcat gacccagcat ctcatccata tggttatcaa caaattactc
     7621 ctgcagcaaa gtctcagccg ttacccatgc caaatattct ggcaaaccct cgttcacatg
     7681 gatacctaca atctctggaa gttcataagg atgcagcatc cgaagcttag tctcacaggc
     7741 ggcatatccc ttacaactgg ttttaatggt caagccaacc tcttcggcac gctccacttt
     7801 tccttgccat tgataaatcg aggtgacagg gcttgaaata ttgacacatg ccgccaactt
     7861 ctctgtaacc agcgtttgag caatatgctc ggcacatgcc ctgttcggca catgcgtaaa
     7921 cacgattata atttcctctt ttggcgtcat cggcgctgag tcaccaacag gattctgaca
     7981 tgcgtttttt catctttgct attctactgg cttttccgtt tctggagata gccgtcctga
     8041 tcgaactctg tcagcgctat ggctggtggg tcttggtgta tttggtcatc atcggctatc
     8101 tgggactcaa attgattcgc agcgaaaaag atctcattgc ggcacgtatg ttccagcaaa
     8161 tgagcgccgg tggcaatcca gtttcagcct tgctctcaac tgcccgtaat atgtttgccg
     8221 gggtattgtt actgattcca ggggtcatta gtgatgcgct ggctgttatc ctgttactga
     8281 tcccaaccaa gaatccaggc ttggggaaaa gtggccagaa caatggtacg tattatcaaa
     8341 caagtgccaa tgacgaggtg atcgaaggtg aatttaccga agtcaaagaa accaaagcgg
     8401 cggataatgt cgttcacttg ccacgcaagg actaatttca aagaaaacat tagcttatga
     8461 atcgtgtctt gattgaaaac agcggccaca ctttcgacgt gcggcccagc caaacggtcc
     8521 tagaagccgc cattgaggct ggcatcaata tgccatacgg ctgtcgtaat ggtgcctgcg
     8581 gtgcctgtaa aggcaaaatc ctgacgggta aagtcatgca tgacgactat caaggcaacg
     8641 ctctcaccga tgccgagctg gcagcagggc aagctttgtt ctgttgtgcc agaccgctgg
     8701 aagatctggt cattgaatgt cgccagatcg acatggtgca aggtatcaaa ccacgtatct
     8761 tacccgtccg cgtacaaaac aaagagcagt tcggtgacga tgtcatggtg ctgcatttac
     8821 aactgccagg caccgagcag ctctccttta tggcaggaca atatgtcgaa ttcctgctta
     8881 aagatggccg tcgccgcgcg ttctcaatcg ccaatgcacc gcatgaaaaa ggctttcttg
     8941 agctgcatat ccgcaaaatt gcgggtggtc actttaccga acaagttttt aacgagatgc
     9001 ccgtcaaaac gatcctgcgc atcgaagctc ctcttggcag ctttttcctg cgcgaacaaa
     9061 atgaaaaacc catcctcatg gtggctggcg gcaccggctt tgcaccggtt aaaggcatga
     9121 tagaacacat gattttcaac cagatccaac gcccggtata tctctaccgt ggcgcgcgac
     9181 aagttggcga cttgtatatg gatgagctct gccaacgctg ggcgcaattt atgcccaata
     9241 ttcaatatat tccagtgatc tcagatggca aagagggaga tggctgggat ggccgccgtg
     9301 gcttagtgca tgaggctgtt ttggaagatc atgccgattt gagtggattt gaagcttacg
     9361 tgtgtggcgc accgggcatg gtggatattt cgaaagagac gtttgtgaag caaggcctac
     9421 cagaagaaga attttat
//
LOCUS       DATMSK010000147         4730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_293100,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000147 DATMSK010000000
VERSION     DATMSK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293100"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="VNZ84_05005"
     CDS             complement(<1..112)
                     /locus_tag="VNZ84_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66347.1"
                     /translation="MKIAIAGTGYVGLSLAVLLAQKNEVIALDIVPEKVAL"
     gene            complement(228..1181)
                     /gene="miaA"
                     /locus_tag="VNZ84_05010"
     CDS             complement(228..1181)
                     /gene="miaA"
                     /locus_tag="VNZ84_05010"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWU66348.1"
                     /translation="MTNRQPVLPPAIFLMGPTASGKTAATIALAERLPVEIISVDSVL
                     VYRGMDIGTAKPDAETLRLAPHHLIDLITPLEQYSAAQFAKDALALMHAATAKERVPV
                     LVGGTMLYFNALQHGLSDLPEADAAIRAQIEAEAQAVGWPAMHAQLASIDPTVAAKIH
                     TTDSQRIERALEVYRISGKPMSVLRAQSTKVSLPFNILKLALMPSDRSVLHQRIAERF
                     QLMLKQGFVDEVKTLLADYPQLTIESPSMRCVGYRQVLQYLNGDIDLPTLGEHGVYAT
                     RQLAKRQLTWLRGMDDVRVLNCLRLDWTEEMLGAIALHITN"
     gene            complement(1234..1605)
                     /locus_tag="VNZ84_05015"
     CDS             complement(1234..1605)
                     /locus_tag="VNZ84_05015"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWU66349.1"
                     /translation="MTDQVLTEQEIQQHFSESLPHWVYEEGWIRRKYKTNSWKGTLMV
                     VNTVGHLAEAAWHHPDLTVSYAFVIVKLCTHSAKGITMKDIELARKIEEVVQWQPQLQ
                     AGSALEGTPQGDLRFSYVKYD"
     gene            complement(1684..2526)
                     /locus_tag="VNZ84_05020"
     CDS             complement(1684..2526)
                     /locus_tag="VNZ84_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HWU66350.1"
                     /translation="MIISVQQAYLNACLAELEALKPGNVHVFADGHGMQVQDFIHSAE
                     VSAPALCDDQVFGVRSLGQRILDALHATYGKVGCNTNLGIVLLAAPVVQAWANYPQQP
                     LQYGLQQVLQQTTIADAACVYQGIRLVNPAGMGQRQEHDLSEAPQIDLLEAMRLASAQ
                     DMVARQYVDGYATIFSAALPLYRELMRRWERPAWALTGVYLYWLSTFPDSHIARKYGA
                     AMAGSVQQTAQMHYQAFLALENPKHYLPELLKWDHVLKEQKLNPGTSADLTVITAMCE
                     ALAE"
     gene            complement(2553..3446)
                     /locus_tag="VNZ84_05025"
     CDS             complement(2553..3446)
                     /locus_tag="VNZ84_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWU66351.1"
                     /translation="MKVVPIFTDEVAQAGGWHGQSLAQAFARRGWQALMVSLDSCHVS
                     IIEQQVTVHIPGLMQSAPIAFVRGVAAGTTQQIITRMNILHTLQRQGMIIYNQARAIE
                     ITVDKGLTSQLLAEQGVATPSTWICEHRGVAHSLMQQAMDAGKSLVIKPLFGSQGKGV
                     RLIDPQQFALPQDPFVDGVYYLQEKIECGLHQHDYRVFVVRGEPVAVMKRQGDGWLHN
                     VARGAKCTACDEPDVASLGVKAAQAINIDYAGVDIMRDKHGKLWVIEVNSIPAWRGLQ
                     SITSVEIADVLVNDLLRLTKL"
     gene            complement(3529..4503)
                     /gene="mch"
                     /locus_tag="VNZ84_05030"
     CDS             complement(3529..4503)
                     /gene="mch"
                     /locus_tag="VNZ84_05030"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HWU66352.1"
                     /translation="MSVTASNSTSISVQHYSAPLVAHLIANAPALGCAVYTHETGATI
                     VDAGIQAAGGLEAGRIIAEICMGGLGKVALQQVPQFAHWPFSVVVTATQPVIACLGSQ
                     YAGWALSHEKFFSLGSGPARSIAQREDVFKDINYRDKGEQTVLVLETDKVPPVQVIEK
                     VARDTGLPANQLTFILTPTRSVAGSLQVTARVLEVALHKCHTLHFDLNAIVDGYGVAP
                     VPPPSPDFIVGMGRTNDAILFGGFVQLFVNVDDAAAEQLAKQLPSSASKDYGRPFAEV
                     FKAVNMDFYQIDPMLFSPAKVSVTNLKSGRTFFAGKFNEDLLNQSFGS"
     gene            complement(4469..>4730)
                     /locus_tag="VNZ84_05035"
     CDS             complement(4469..>4730)
                     /locus_tag="VNZ84_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66353.1"
                     /translation="FPRQKAHASHCIAYARQEIDLSQLDFPDWLEDRPSGGTIATGQP
                     FCSMYAAGDSDSEVRQALQNKKTRLEKLWGTYVCDRIEFNIH"
BASE COUNT         1203 a         1262 c         1113 g         1152 t
ORIGIN      
        1 gcagcgccac tttttccgga acgatatcca aagcaataac ttcatttttt tgcgctaaca
       61 aaaccgcaag cgataagcct acataccccg taccagcaat agcaattttc ataggaacag
      121 ctcacaaaat actaacaata atattttcat aggcaataac agccaaacta aatcttcaaa
      181 taacaatgac aatatcaagt attcataaaa ttcagtaaaa ttctagttta attagtgatg
      241 tgcaatgcaa tcgcacctaa catttcttct gtccagtcca aacgcaagca attcagcacc
      301 cgcacatcgt ccatcccgcg tagccatgtg agttgacgct tggccagttg acgtgtggca
      361 tacacgccat gctctcccag cgtgggtagg tcaatatcac cattcagata ttgcagcacc
      421 tgacgatagc cgacgcaacg catgctggga ctttcgattg tcagttgtgg gtaatcagcc
      481 aatagcgttt taacttcatc cacaaagccc tgtttaagca taagttggaa gcgttcagcg
      541 atccgttgat gtaacacact gcggtcactt ggcatcaacg ccagtttaag gatattaaac
      601 ggcaacgaga cttttgttga ttgcgcacgc aatacgctca tgggcttgcc gctgatgcga
      661 tagacttcta gcgcccgctc aatccgctgg gaatcggtgg tatgtatttt ggcggcaacg
      721 gttggatcta tgctggctaa ttgggcatgc atggctggcc aaccaacggc ctgtgcttct
      781 gcttcaattt gggcgcgaat ggcggcatct gcttctggca aatcactcaa cccatgctgc
      841 aaggcattga aatagagcat ggtaccaccg accaacaccg ggacacgttc tttggctgtt
      901 gcagcatgca ttagcgccaa tgcatctttg gcaaattgtg ctgcggaata ttgctccagc
      961 ggagtaataa gatcaattaa gtgatgtggc gccagacgca aggtttcagc atcaggcttg
     1021 gcggtgccta tatccatgcc ccggtaaacc agtacggaat ccacactgat aatctcaacc
     1081 ggtaagcgct ctgccaatgc tattgttgct gccgttttgc cgctggcagt ggggcccatt
     1141 aaaaatatgg ctggcggcaa tacaggctga cgattagtca tacaacactc aacaacaaaa
     1201 cagacgaaaa atgatcaata aaaatggttt ggattagtcg tatttgacat agctgaaccg
     1261 caagtctcct tgcggtgtgc cttccagggc tgatcctgct tgtaattgtg gctgccattg
     1321 cacgacctct tcaattttgc gcgccagctc gatatctttc atggtgatgc ctttggcgct
     1381 atgtgtgcaa agtttcacta tgacaaacgc gtaggaaacg gtgagatcag gatggtgcca
     1441 ggccgcctca gcgaggtgtc ccacagtatt caccaccatg agcgtgccct tccagctgtt
     1501 ggttttatat ttacggcgta tccagccctc ttcatacacc cagtgcggca atgactcaga
     1561 aaaatgttgt tgaatttcct gctcggtgag tacttggtcg gtcatggcag actcctgatc
     1621 attgttttga aaggctacca gtatactcca gtgacgccag tgcactcact atgaataggg
     1681 cgcctactct gctaaagctt cacacatagc cgtgatcacg gtaaggtctg cactggtgcc
     1741 aggattgagc ttttgctcct tgagcacatg atcccacttt agtaattcag gcaggtaatg
     1801 tttaggattt tccaaggcaa gaaaggcttg gtaatgcatc tgtgccgtct gctgaacgct
     1861 accagccatt gccgccccat atttacgcgc aatgtggcta tcaggaaaag tggatagcca
     1921 atataagtaa acacccgtca gtgcccaggc tggacgctcc cagcgcctca taagctctcg
     1981 gtaaagtggc aacgctgcag aaaagatggt ggcatagcca tcgacatatt ggcgtgcaac
     2041 catatcttgc gcgctggcca gacgcatcgc ctctaacaaa tcaatctgtg gcgcttctga
     2101 taaatcatgt tcttggcgct gccccatgcc agccgggttc accagcctga taccttgata
     2161 cacgcaggct gcatcagcaa tggtggtttg ctgtagcact tgttgcaaac catattgcaa
     2221 tggttgttgc ggatagtttg cccaggcctg gaccactgga gctgccagta acacaatgcc
     2281 cagattggta ttgcagccaa ctttgccata cgtggcgtgt agggcgtcta aaatacgctg
     2341 ccccagagaa cgtacgccaa aaacttggtc atcacataag gccggtgcag atacctcagc
     2401 actgtgaata aagtcttgga cctgcatgcc atgcccatcc gcaaaaacat gtacattgcc
     2461 gggcttcagt gcctcaagct ctgccaggca ggcattcaag taggcttgtt gtacagagat
     2521 gatcatcacc aatatttaaa gcttagccaa tattaaagtt tcgtcaggcg caacagatca
     2581 ttgaccagca catcagcgat ttcaaccgag gtgatacttt gcaaaccccg ccaggcaggg
     2641 atgctgttga cctcaatcac ccataacttg ccatgcttgt cgcgcatgat atcgactccg
     2701 gcataatcaa tattgatcgc ctgcgcagcc ttgacaccaa gcgaggcgac atcaggttca
     2761 tcacaagcag tacatttcgc acctcgggct acgttgtgca gccaaccgtc accttgtcgc
     2821 ttcatgacag ccaccggctc accgcgcacg acaaatacac ggtaatcatg ctgatgcaga
     2881 ccacactcaa ttttttcttg cagatagtac acaccatcga caaacggatc ttgcggtaag
     2941 gcaaattgtt gcggatcgat taaacgcacg cctttgccct gggaaccaaa caagggcttg
     3001 atgactagtg acttcccggc atccattgcc tgctgcatca atgaatgcgc cactccacga
     3061 tgctcgcata tccaggtcga aggtgtcgca accccctgct ctgccagcaa ctggctggtt
     3121 aaacctttat ctacggtaat ttcgattgca cgcgcctgat tgtaaatgat catgccctga
     3181 cgttgcaggg tatgcaggat attcatacgc gtaataattt gctgtgttgt gccagcggcc
     3241 accccccgca caaaggcgat gggtgcgctc tgcatcaaac ccgggatatg tactgttacc
     3301 tgctgttcga taatactgac atggcagcta tccagtgaca ccatcaatgc ttgccagcca
     3361 cgccgcgcaa atgcctgcgc caggctttgt ccgtgccagc cacccgcttg ggcgacttcg
     3421 tcggtaaaga taggaacaac cttcatatcc actctaaaat aatgacgcgg gaaccgtgca
     3481 tcagatgcat tgtatcccgc gcaaaagtgt ctttaattaa ctttaaactc agctaccaaa
     3541 tgattgattc aaaaggtctt cattgaactt cccagcaaag aaagtacggc cggattttaa
     3601 gttggtcacg ctcaccttgg ccggtgagaa cagcatgggg tcaatttggt aaaagtccat
     3661 attgacggct ttgaatacct cggcaaacgg gcggccataa tctttagatg cagaggaagg
     3721 taactgcttg gccaactgtt cggcggcggc gtcatccaca ttgacaaata gctggacaaa
     3781 gccaccaaac agaatcgcat cattggtgcg gcccatcccc acaatgaagt ctggtgaggg
     3841 aggcggtacc ggggctacgc cataaccatc cacaatcgcg ttcaagtcaa aatgcaaggt
     3901 atggcattta tgcagtgcga cttccagcac gcgtgccgta acttgcaacg aaccagccac
     3961 gctacgggtg ggtgtcagga taaaagtcag ttgattggcg ggcaatccgg tatcacgggc
     4021 cactttttca atcacctgaa caggaggtac cttatcggtt tccagcacca gaaccgtttg
     4081 ttcgcctttg tctctgtagt taatgtcttt aaatacgtct tcacgctgag caatcgagcg
     4141 tgccgggccg ctgcccaatg agaaaaattt ctcatgcgac aaggcccaac cagcatactg
     4201 gctgcccagg caggcaatga caggttgggt agccgtcacg acaacactga atggccagtg
     4261 cgcaaattgc ggcacttgct gcaatgccac tttacctaaa ccacccatgc aaatttcggc
     4321 gataatacgc cccgcttcca ggccaccagc ggcctggatc cctgcatcca ctatcgtggc
     4381 gcctgtttca tgcgtataca ccgcacagcc caaggccggc gcgtttgcga tcaggtgcgc
     4441 caccagtggt gcgctatagt gctgaacgct aatggatgtt gaattcgatg cggtcacaga
     4501 cataagtccc ccataatttc tctaatcgtg tttttttgtt ctgcaaagcc tgtcgcactt
     4561 cgctatctga gtcccctgct gcatacatgc tgcaaaaggg ttgccctgtg gcaatcgtgc
     4621 caccactggg ccggtcttcc agccagtcag gaaaatccag ctggcttaaa tcaatctctt
     4681 gccgtgcata cgcaatacaa tgactggcat gcgctttctg ccgcgggaac
//
LOCUS       DATMSK010000148         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_151662,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000148 DATMSK010000000
VERSION     DATMSK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151662"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VNZ84_05040"
     CDS             <1..488
                     /locus_tag="VNZ84_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HWU66354.1"
                     /translation="IERSTGTWALVFLLLSLSITPLRHWTRQAWLIAVRRLLGLWMFA
                     YACLHVTSYVWLDYQFDWLNIVKDIIKHPYVLVGAAALLLTLPLAATSNQKAIKLLKQ
                     RWKSLHALVYAIGVLVLLHYWWLVKKDVTEPVIYSIIFAVLMLFRWQHSWKNDSIHPI
                     L"
     gene            809..3010
                     /locus_tag="VNZ84_05045"
     CDS             809..3010
                     /locus_tag="VNZ84_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU66355.1"
                     /translation="MASPALSATFEKWQAQVNSSGFYIAAETDNTGHCLSVLASQSPE
                     PVRDLWAYLPVEARDPIRAILSPPSTEIKQRTLHIACHDSQHRWLECCVYYFGLDTGG
                     HRSLLLVRETTSEHQSATVLQQEKLRAMAYERIKFSILELILKEQDAVRILEKMVHDI
                     ESLHTDVYCAIVLTRNLGKLVQTVIAPSLPQAMLHTLRQLQVEAGNGSLATAIATRQR
                     VIIENVMTHPYWINHREAALVSNIQAGWSEPILSAEGEVLGAFAIYYRKPQTPSVLEQ
                     SMIEQAAKLVSIVLDRHLSQESIQNLAYLELVTGLPNRRQVYEILHQLRTQHASENYW
                     GVVYIDLDHFKWINEAHGHAAGNSLLQQVAERLQKVVEPHFIACMGGDEFVVLLSGLA
                     PQADKALQQVWAMQRKLQKVFDRPFKVAGQKLSMTASMGLCSFDAMSAEKTDYVKAAD
                     IAMHKAKQAGRNHACLYEQGMQTEIATQVMLETELREAIAQQQFRLYYQVQVDHTGRP
                     FGAEVLLRWHHPQRGVISPVQFISVAETSGLIIEMGMWVLDQACAQIAAWQLSPKTRE
                     LSLSVNISARQFRQANFVAMVKACIQRHQINPNALRLELTESLLLENVDDAVHIMEEL
                     SQFGIQFSLDDFGTGYSSLQYLKKLPLYQLKIDRSFVRDIVTDSHDRTIVRTIIAMAQ
                     SMYLSVIAEGVETQEQMELLLNNGCRRYQGFLFGQPMPIASFEQWLQHALV"
     gene            complement(3007..3894)
                     /locus_tag="VNZ84_05050"
     CDS             complement(3007..3894)
                     /locus_tag="VNZ84_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWU66356.1"
                     /translation="MYRRRPLDDEDESHERWLISYADFITLLFAFFVVMYATSTINLN
                     KYRALSDAVVTAFQGKPGQTTSLDAQTQMTLQNQSSVLKPLPLSYLYQEKKQRDQEKV
                     RALGQHMANILAPWIEQKLISVFQSDLGIEMDIQSNLLFNGNQATYSPQASTVLNAIS
                     EPLKNEYRTIQVEGHSDKQVFSGQSDAETKRWELTAAQAAHIAASLVAQGVASKRLSA
                     IGLAETKPVSSSDNELAQALNTRVTIRILTAESSAQLTSNIANRQEITPGSVETEPGL
                     ADKASSASPIQQGSGEHPH"
     gene            complement(3894..>4351)
                     /locus_tag="VNZ84_05055"
     CDS             complement(3894..>4351)
                     /locus_tag="VNZ84_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWU66357.1"
                     /translation="IKREQDPFLRKGLQLLVDGTPADRIREVCAIDMYYYELQEREAA
                     KIWSAAGGYAPTVGILGAVLGLIHVMENLSDPAKIGSGIAVAFVATIYGVALANLVFL
                     PISNKLKGHIQHEMSRREMLLNAWVSIARGDNPKLVSERLESYLHLRET"
BASE COUNT         1064 a         1038 c         1103 g         1146 t
ORIGIN      
        1 tcatagaacg ctccaccggc acctgggcgt tagtgtttct gctgttgtcg ttaagtatca
       61 cgccgttaag gcactggacc agacaggcat ggctgattgc cgtgcgccgt ttattgggat
      121 tgtggatgtt tgcctatgcc tgcctgcatg tgaccagcta tgtgtggctg gattaccagt
      181 ttgactggct aaatattgta aaagacatta tcaagcaccc ttatgtgctc gtgggggccg
      241 cggcattact tttgacactg ccattagccg cgacctcaaa ccagaaagca atcaagctac
      301 ttaagcagcg ttggaaaagc ctgcacgcgc tggtttatgc catcggcgta ttggtgttat
      361 tgcattattg gtggctggtg aaaaaggatg tcaccgaacc agttatatac agcatcattt
      421 ttgctgtact catgcttttc agatggcagc acagttggaa aaatgattcg atacatccca
      481 tattgtgaaa atggttgtca tgagtgaaaa gccataaagg cctgtttcca tcagcaattg
      541 tcacatcttt gtgacgatcg gccctgacag ttgttaatgt ctgcgctatc aagttccttt
      601 gtcttgacat cacatggctt tttagacaca ttacgaagaa tataaagtga gcagtcaaca
      661 tgattgactc ccaactaaaa ttattcgggg gtgtagcatc ttaacaaccg gcaggcagag
      721 ggtgcctatg cgtcggcagt tagattgtgc actgcggttg tgaaagtatg cgatgtctac
      781 agtaaatcta ctgccaaatg cagttgtttt ggcaagtcct gccttgagtg cgacgtttga
      841 aaagtggcag gcacaggtta attcttccgg tttttatatt gcagcagaaa cggataacac
      901 aggacattgc ctctctgtgt tagccagcca gtcacctgaa ccagttcgtg acctgtgggc
      961 ttacttgcct gttgaggcac gcgacccgat ccgggcaatc ctgtctccac cttctactga
     1021 gatcaaacag cgcacgttgc atattgcatg tcacgatagt cagcatcgct ggctggagtg
     1081 ctgcgtctat tatttcgggc tcgacactgg cggtcatcgc agcctgttgt tggtccggga
     1141 aacaacaagt gagcatcaat cagccaccgt attgcagcag gaaaagttac gcgcaatggc
     1201 ttatgagcgt attaaatttt ccattcttga gttgatcctg aaagaacagg atgcagtacg
     1261 gattttagaa aagatggtgc atgatataga aagcctgcac acggatgtct attgcgccat
     1321 tgtgcttacc aggaacctgg gtaaactggt gcaaacagtc atcgcgcctt ccttacccca
     1381 ggcaatgctg cataccttgc ggcagttaca agttgaggct ggcaatggct cactggcaac
     1441 tgccattgcc accaggcaac gtgtcatcat cgaaaatgtc atgacgcatc cttactggat
     1501 caaccaccgt gaagccgcgc tggtttccaa tatacaggca ggctggtcag aaccgatttt
     1561 gagtgcggag ggggaagtcc tcggcgcttt cgctatttac taccgtaaac cgcagacacc
     1621 tagcgtgctt gagcaatcca tgatagagca ggcggccaaa ctggtcagta tcgtgctgga
     1681 ccgccattta tctcaagagt ctatccaaaa cctggcatac cttgagcttg tcacaggctt
     1741 acccaatcgc aggcaggtgt atgaaatcct gcatcagctg cgtacgcaac acgccagcga
     1801 aaactattgg ggggtcgtat atatagacct ggatcacttt aaatggatta atgaggccca
     1861 tggccatgcc gccggtaaca gcttattgca gcaagtggct gaacgtttgc aaaaagtcgt
     1921 cgagccacat tttattgctt gtatgggagg tgacgaattt gtcgtattgc tttcaggact
     1981 tgcgccgcag gcagataagg cattgcagca ggtatgggct atgcagcgta agctgcaaaa
     2041 agtatttgat cgcccattca aggttgctgg acaaaagcta tccatgacgg ccagcatggg
     2101 cctatgcagc tttgatgcca tgagcgcaga aaaaacggac tatgtaaaag ccgctgatat
     2161 cgcgatgcat aaagcgaagc aggctgggcg taatcatgcc tgtctttacg agcaggggat
     2221 gcaaaccgaa attgcaacgc aggtcatgct ggaaactgag ctccgtgagg ccattgcgca
     2281 gcaacaattc cggttatatt accaggtgca ggtcgaccat accggccggc catttggtgc
     2341 tgaggtctta ttaagatggc atcacccgca gcgcggtgtg atttcgcctg tgcaatttat
     2401 ctcggtagca gaaacaagtg gtctcatcat tgaaatggga atgtgggtat tagatcaggc
     2461 gtgtgcacaa attgctgcct ggcaactatc gcccaaaacc agggagctca gcctgtctgt
     2521 taatatcagc gcacgccaat ttaggcaggc caattttgtg gccatggtta aagcttgtat
     2581 tcagcgtcac cagattaacc ccaatgcctt acgtctggag ctcaccgaga gcttgttact
     2641 ggaaaatgtg gatgatgccg tgcacatcat ggaagagtta agccaatttg gcattcagtt
     2701 ttcactggat gactttggca caggctattc ttctctgcaa tacctgaaaa aactgccgct
     2761 ataccagctt aaaattgacc ggtcgtttgt gcgggatatc gtcactgaca gccatgaccg
     2821 caccatcgta cgtaccatta tcgccatggc acaaagcatg tatcttagcg tgattgctga
     2881 aggtgtagaa acacaagagc agatggagct gctgctgaat aatggatgcc gacgttacca
     2941 aggcttcctg tttggacagc cgatgccgat tgcgtcattt gagcagtggt tacaacacgc
     3001 gctggtttaa tgcgggtgct caccggaccc ttgctgtatc gggctggcac tgcttgcttt
     3061 atctgctaaa cccggctcgg tttcgacact accaggggtg atctcttgcc ggttagcaat
     3121 attgctggtt agttgtgcgc tgctttcggc cgttaaaata cgtatcgtca cccgggtatt
     3181 cagtgcttgt gccagctcat tatcgctgct tgaaacgggt tttgtttcag ccaggccaat
     3241 ggcagataaa cgtttactgg caaccccttg tgcgaccagg ctggctgcga tgtgcgctgc
     3301 ttgagcagcg gtcaattccc agcgtttggt ttctgcatcc gattgcccgc taaatacctg
     3361 tttgtcgcta tgaccttcaa cttgtatggt ccgatactcg tttttcagtg gctcactgat
     3421 cgcattcaaa accgtgcttg cctgcgggga gtaagtcgcc tggttgccat tgaataatag
     3481 gttactctgg atatccattt ctataccgag gtcagactga aacacgctga tcagtttttg
     3541 ctcaatccag ggcgcaagga tattggccat gtgctgacca agtgcgcgca ccttttcctg
     3601 gtcacgctgt ttcttttcct gatatagata gcttaatggc aggggtttaa gtacagaact
     3661 ctggttctgc agggtcattt gtgtttgcgc atctagcgat gtggtttgcc ccggcttgcc
     3721 ctgaaaggcg gtgaccacgg catcagacaa tgcacggtat ttattcaggt tgatggtcga
     3781 agtcgcatac atcaccacaa aaaaggcgaa tagcagggtg ataaagtcgg cataagagat
     3841 cagccaacgt tcatggcttt catcttcatc atccaatggc ctgcgtctgt acattatgtt
     3901 tcccgtagat gcaggtatga ctccaggcgc tcgcttacca gtttaggatt gtcaccgcgg
     3961 gcaatggaga cccaagcatt cagcaacatt tcccgtctgg acatttcatg ctggatatgg
     4021 cccttgagtt tgtttgaaat cggtaaaaat accaggtttg ccaaggcaac cccgtaaatg
     4081 gtggcaacga atgccacagc gataccgcta ccaattttgg caggatcaga caggttttcc
     4141 atgacatgga tcaggcccag gactgcaccc aaaatgccaa ctgtaggcgc atagccccca
     4201 gctgcagacc aaatttttgc tgcttcacgt tcttgcagtt catagtaata catatcaatg
     4261 gcacagactt cacggatgcg gtcagcaggc gtgccatcta ccagtagttg caacccttta
     4321 cgcagaaaag gatcttgttc gcgcttgata t
//
LOCUS       DATMSK010000149         4711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_246213,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000149 DATMSK010000000
VERSION     DATMSK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246213"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..931
                     /locus_tag="VNZ84_05060"
     CDS             5..931
                     /locus_tag="VNZ84_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWU66358.1"
                     /translation="MQAAELLNASYDVTREFYKDFNPAFVQYWKEKTGETVTINQSHG
                     GSTKQARSVVDGLPADVITMNRSADIDILAQKGKLIPANWQTRLPNNSVPSASPTVFL
                     VRKGNPKQVKDWDDLVRPGVTSVIPNPKTSGNGKFSYLAAWGYITHKGGDATKARQFV
                     TELFKHVPVLDTGGRGATTTFAQREIGDVLVTFENEVYLLQKELGEDKYEVVYPSVTV
                     LAENPVALVDKVVDKKVTRKVAQAYLEFHFSPQGQAIAAKHYLRPQVTEVAQPTFKKL
                     NTFTVKQAFGSWHEAENTHFSDGGIFDQIYQK"
     gene            958..1827
                     /locus_tag="VNZ84_05065"
     CDS             958..1827
                     /locus_tag="VNZ84_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU66359.1"
                     /translation="MTQRQPNQEPQHVIQLRAELKQALSLDLEELGLLEASSGEYEST
                     FIGVHLKTAFQPIYDAKQGDIYGYEALIRPSLFGTLGSTPEFAFTYAEQSGKLVQFDR
                     VCRSQHVLNYRAIHQENGLLFLNVHPKLLLEVSAHGKVFEQILHKYSVPTHRVVLEIN
                     ESLIEQDKHLISAIENYRSLGYRIAFDHVGGHQSKLYRLWNAHHDYIKFDVSVIHQAA
                     QQPALAKALKGLVSTVQDLGSIPVILGIETQQQLDIAIASGATVLQGNLLAVPVVAKV
                     INDQYQHRSPKVA"
     gene            1936..2769
                     /gene="cysT"
                     /locus_tag="VNZ84_05070"
     CDS             1936..2769
                     /gene="cysT"
                     /locus_tag="VNZ84_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HWU66360.1"
                     /translation="MASHKKKHLLPGYGLSLGVTLVYLSLIVLIPLAAVFLRTTELSF
                     HEFWAVVTTPRVVATYKLTFGASFLAAVINLVFGLLTAWVLVRYQFVGKKILDALVDL
                     PFALPTAVAGIALTAIYAPNGWLGQWLEPHGIKVAFTPLGVVVALTFIGLPFVVRTVQ
                     PVLEDFSAEAEEAAASLGANRWQTFYKIILPAIWPALLTGFSLAFARAVGEYGSVIFI
                     AGNMPMISEITPLMIITKLEQYDYAGATAIAVVMLVISFVLLLLINLLQWWSNHRHGN
                     K"
     gene            2809..3699
                     /gene="cysW"
                     /locus_tag="VNZ84_05075"
     CDS             2809..3699
                     /gene="cysW"
                     /locus_tag="VNZ84_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HWU66361.1"
                     /translation="MHTTQFQQYQGKVASKRATSEPAWVRWSLIGLALAFLGLFLLVP
                     LAAVFAEALRKGWGVYLAAITEADALSAMKLTLIASAISVPLNLVFGVAAAWAITKFD
                     FKGKSFLITLIDLPFAVSPVIAGLIFVLIFGLQGWFGEWLIDHDLKVVFAVPGIVLAT
                     IFVTFPFVARELIPLMQAQGKEEEEAALVLGASGWKMLWHVTLPNVKWGLIYGVILTN
                     ARAMGEFGAVSVVSGHIRGLTNTMPLHVEILYNEYNYAAAFAVASLLTLLALVTLVLK
                     TYVEWQASRDAAHQEKEVTA"
     gene            3701..4678
                     /locus_tag="VNZ84_05080"
     CDS             3701..4678
                     /locus_tag="VNZ84_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HWU66362.1"
                     /translation="MSITIQQINKGFGQFSALRDINLQVPSGELVALLGPSGCGKTTL
                     LRIIAGLETPDNGQVLFDGVDTTHADVRERQVGFVFQHYALFRHMTVFENVAFGLRVR
                     PKESRPSNAEIKKRVHDLLKLVQLDWLADRYPPQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKDLRRWLRRLHDELHVTSVFVTHDQEEALEVADTIVVMNHGQIEQV
                     GSPQAVYDQPATPFVYEFLGNVNAFETPQGKGFVRPYEIAVSREADANSIPGALTYVH
                     SVGSLVRLEIKRTDNEQYVDVELGREQFNLLDFKQGETVYLKPTQLRVF"
BASE COUNT         1110 a         1150 c         1251 g         1200 t
ORIGIN      
        1 gtcactgcaa gccgccgagt tgttaaatgc ttcttatgat gtgacgcggg aattctataa
       61 agactttaac ccggcttttg tgcagtactg gaaagaaaaa accggggaaa cggtgaccat
      121 caaccagtca catggcggct cgaccaaaca ggcacgttca gtagtcgatg gtttgcctgc
      181 ggatgtgatt accatgaacc gcagtgcgga cattgatatc ctggcgcaaa aaggaaaatt
      241 gatcccggct aactggcaga cgcgtttgcc gaataacagt gtgcctagtg cttccccaac
      301 cgttttttta gtccgcaaag gtaaccctaa acaagtgaaa gactgggatg acctggtgcg
      361 tcccggcgtg acttcagtga ttcctaaccc aaagacgtct ggcaatggta aattcagcta
      421 cctcgccgcc tggggctata tcacgcacaa agggggcgat gccaccaaag cccgccagtt
      481 tgtgactgag ctatttaagc atgtgcctgt actggatacc ggtggtcgcg gtgcaactac
      541 cacttttgcc caacgcgaaa ttggcgatgt gctggtgact tttgagaacg aagtctactt
      601 gctgcaaaaa gagctgggtg aggataaata tgaggtggtg tatccctctg tcacggtgtt
      661 ggctgaaaac ccggtcgcgc tggtagacaa agtagttgat aaaaaagtca cacgcaaagt
      721 cgcgcaggct tacctggagt tccatttttc accacaaggc caggcgattg ctgccaagca
      781 ttacttgcgt ccgcaagtga ctgaagtggc acaacccaca ttcaagaagc tgaatacatt
      841 tacggtcaaa caagcgtttg gcagctggca cgaagctgaa aatacccatt ttagcgatgg
      901 cggcattttc gaccagattt atcagaaata gccgttgcat cgcaggaagg aatcatcatg
      961 acacaacgtc aacctaacca ggagccacag cacgtcatac agctacgtgc agaattaaag
     1021 caggcactgt cgctggacct ggaggagctg gggctgctgg aagccagttc gggcgaatat
     1081 gagagcacct ttatcggcgt gcacctcaaa accgctttcc agccgattta tgatgccaaa
     1141 caaggagata tctatggcta cgaagccctg atccgcccat ccttgtttgg tacgctgggc
     1201 agcacgccag agttcgcatt cacctatgcc gagcaatcgg gcaagctggt gcagtttgac
     1261 cgagtatgcc gctcgcaaca cgtgctgaac taccgcgcca tccatcagga aaacggcttg
     1321 ctgtttttga acgtgcatcc caagttgttg ctggaagtca gtgcacacgg caaggtgttt
     1381 gagcagattt tacataagta ttctgtaccc acgcataggg tcgtgctgga gattaatgag
     1441 tccttgatcg agcaggataa acacctgatt tcagccattg agaactatcg ttcactgggt
     1501 taccgcattg cgtttgacca tgtaggaggc caccaaagca agttataccg cttatggaac
     1561 gcacatcatg actacatcaa gttcgacgtg tccgtgatcc accaggcggc gcaacagcct
     1621 gcgttggcca aagccttgaa aggcttggtg tctacggtgc aggacttagg cagtatcccg
     1681 gtgattttgg gcatcgaaac ccaacagcaa ctggatattg cgattgcgtc tggtgcgacg
     1741 gtgttgcagg gcaacttgct tgcagtgccg gtggtggcca aagtgattaa tgaccagtac
     1801 cagcaccgct caccaaaagt ggcgtaattc ttatttttaa tataacgggc gcttataaat
     1861 taatgattat ttattcttta atgagttaaa agaataaggc tatattagcg cctgtctaac
     1921 tggttaccat acatcatggc atcacataaa aagaaacatt tacttcccgg ctacgggctg
     1981 tcactagggg tgacactggt ttatttaagc ctgattgtat tgattccctt agccgcggtt
     2041 ttcttacgca ccaccgaatt aagctttcat gagttttggg cagtggtcac cactcctcgc
     2101 gtggttgcaa cttacaaact cacctttggc gcttccttcc tcgccgcggt catcaacctg
     2161 gtgtttggtt tgctgacggc atgggtgttg gtgcgctacc agtttgtggg taaaaaaatc
     2221 ctcgatgccc tggtggacct gccatttgcc ttgccgaccg cagtggccgg tattgcactc
     2281 acggcgattt atgcccctaa cggttggtta gggcaatggc tggagcctca tggcattaaa
     2341 gtcgctttta caccgttggg tgtcgtggtg gcgctgacct ttatcggctt gccgtttgtg
     2401 gtgcgtaccg tgcagcctgt gctcgaagac ttcagtgccg aagctgaaga agccgctgcc
     2461 agcctgggtg ccaaccgctg gcaaacgttt tacaaaatca ttctgccagc catctggcct
     2521 gcgctactca ccggtttttc attggcgttt gcccgtgcag tcggtgagta cgggtcagtg
     2581 atttttatcg ccggcaatat gcctatgatc tccgagatta cgccgctgat gattattacc
     2641 aagctggagc aatacgacta cgccggagcc acagccattg cggtagtgat gctagtgatt
     2701 tcattcgtat tgctcttgct gattaacttg ttgcagtggt ggagcaatca tcgccatggc
     2761 aataagtaat taagttacgc actcgatatt taattgaata gaacatttat gcataccaca
     2821 caatttcaac aataccaggg caaggtcgct tccaaacgcg ccacatcaga gccagcatgg
     2881 gtgcgctgga gcttgatcgg actggcgctc gcattccttg gactgttttt actggtgcca
     2941 ctggcagctg tatttgctga ggccttgcgt aaaggttggg gcgtttacct ggccgcaatt
     3001 actgaagcag atgctttatc agccatgaag ctgacgctga ttgcgtcagc catttccgtg
     3061 ccattgaatc tggtattcgg cgtagctgca gcctgggcaa ttaccaaatt tgattttaaa
     3121 ggcaaaagct tcctcatcac tttgattgac ctgccattcg ccgtctcacc cgtgattgcc
     3181 ggtttgattt ttgtgctgat ctttggcctg caaggctggt ttggagaatg gctgattgat
     3241 catgacctga aagtggtctt tgcggtacct ggtattgtac tggccaccat ttttgtgacc
     3301 ttcccatttg tcgcacgcga gctgattcca ctcatgcagg cacaaggcaa ggaagaggag
     3361 gaggctgcgc tggtgttagg ggcttccggc tggaaaatgc tgtggcatgt gaccttgcct
     3421 aacgtgaagt gggggctgat ttatggcgtg attttgacca atgcccgtgc catgggcgag
     3481 tttggtgcgg tgtctgtggt gtctggccat atccgcggcc tgaccaacac catgccgctg
     3541 catgtcgaaa ttttatacaa cgaatataac tatgctgccg cgtttgctgt tgcttcattg
     3601 ctgacgctgc tggccttggt aaccctggtt ttaaaaacat atgtcgaatg gcaggcttca
     3661 cgcgatgctg cccatcagga aaaggaagtg acggcttaat atgagcatta ccatacaaca
     3721 gattaataaa ggctttggcc agtttagcgc actgcgcgat atcaacctgc aagtgccatc
     3781 cggcgagctg gtcgcgttgt tggggccttc tgggtgcgga aaaaccacct tgttgcgcat
     3841 tattgcaggc ctggaaacgc cagataacgg ccaggtattg tttgatggcg tagatacgac
     3901 ccatgccgat gtccgtgagc gccaggtagg ctttgtgttc cagcattacg ccctgttccg
     3961 tcacatgacc gtgtttgaaa acgtagcatt cggcctgcgt gtgcgcccca aagaatcacg
     4021 tccatccaac gccgagatca agaagcgcgt gcatgaccta cttaaactgg tccagcttga
     4081 ttggctggct gatcgttacc caccccaact ctcaggtggc cagcgccagc gtatcgccct
     4141 ggcacgtgcc ttggcagtag aaccaaaagt gctgttgctc gacgagccgt ttggcgcact
     4201 cgatgccaaa gtgcgtaaag acctgcgccg ctggttgcgc cgtttgcatg atgagttgca
     4261 cgtgactagc gtgtttgtta cgcacgacca ggaagaagcg cttgaagtgg ccgataccat
     4321 cgtcgtgatg aaccatggcc agattgagca ggtgggctca ccccaagcgg tgtatgacca
     4381 acccgccacg ccgtttgtgt atgagttttt gggcaatgta aacgcctttg aaaccccgca
     4441 aggcaaaggt tttgtgcgcc cttatgaaat tgccgtgagt cgagaggcag atgccaatag
     4501 cattcctggt gctctgactt atgtccactc ggttggctcg ctggtgagac tggaaatcaa
     4561 gcgtacagat aacgagcaat atgtagatgt ggagttgggg agagagcagt ttaacctact
     4621 cgattttaag cagggtgaaa cggtttactt gaagcctaca caattgcggg tgttttagct
     4681 aattcactga ataaaaaggc gccgcgtcaa t
//
LOCUS       DATMSK010000150         4830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_175198,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000150 DATMSK010000000
VERSION     DATMSK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175198"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1388)
                     /gene="hpnE"
                     /locus_tag="VNZ84_05085"
     CDS             complement(30..1388)
                     /gene="hpnE"
                     /locus_tag="VNZ84_05085"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HWU66363.1"
                     /translation="MATPAFSRRKIAIIGAGLAGLSAAHKLLRQGHHVIVFEAAPQAG
                     GRARGVTHGNTTLDNGQHLCIGAYSATLNLLQAARLKPEEVFMRLPLSLHMHAWTHNS
                     QQRMSLVTPAWLPAPLHLLWGLLAARGLDWRSKWQAIRWMLDLKRIGFRLEKDITVAA
                     LLVRGQQTAIAISTLWEPLCLGALNTPIAEASAQVFLNVLQDSFQHRRQDSDFLIVKG
                     DLSTALIQPLLQAIQDLGAEVHLRQAVTSIQPLDTGCLVSTATSTVAFDDVVVAVGPH
                     QLKTIESELVIPRFDYQPITTIYLQFESGFRLPHVVMGLCHGLAQWVFDRGQCCDQPG
                     LLAVVISAHPPLNMGKESLLRQCIEEINRALDMYGKQLSTTPLWTQIITEKRATFSCT
                     PDLSRPPIKTGIPHLFLAGDYVAGRYPATIEGTVRSGHAAAQAIMNGDYSLAESLHKT
                     AR"
     gene            complement(1388..2227)
                     /gene="hpnD"
                     /locus_tag="VNZ84_05090"
     CDS             complement(1388..2227)
                     /gene="hpnD"
                     /locus_tag="VNZ84_05090"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HWU66364.1"
                     /translation="MTPHQYCQQKTRESGSSFTLSFMFLPKEKRDAMTALYAFCREVD
                     DVVDECTDYQIAQTKLAWWKQEIHRLFHETPQHPVTQALQPVIKTFDLKEAHFIEIID
                     GMQMDTQYNRYADFEQLALYCYRVASVVGLLSAQIFGYSDPATLQYAHDLGMAFQLTN
                     IIRDVGEDARRGRIYIPLDEMERAGVTEQQLLNSQASPALQALLLRQIERAELFYDKA
                     LNQLPKADIKPQLPGLMMAAIYRALLQEIKPEPNLVLTHKVMLPPFRKLKLALGAWFK
                     YRK"
     gene            complement(2224..3093)
                     /gene="hpnC"
                     /locus_tag="VNZ84_05095"
     CDS             complement(2224..3093)
                     /gene="hpnC"
                     /locus_tag="VNZ84_05095"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HWU66365.1"
                     /translation="MQAFSKPAQQQDSLTLAHTHYENFPVATLLLPAHLRHAVTVIYQ
                     FAREADDIADEGDASEADRLRALQTYEDELLLTQAYIQPSQPLFLALQQVVKTHRLDV
                     QLLLDLIYAFKQDVVKTRYASFDEVLDYCRYSANPVGRLMLQLYGSDTAQHQLWSDQI
                     CTALQLINFYQDIAIDLQKHGQTGRIYLSQDEMQAAGISEQDLHQQRVDAPWQAFFVH
                     NVLRAERMLLAGKPLGRALPGRIGFELRMMIAGGERILHKLKSCRGDIYHHRPTLKAY
                     DWPLILLKALFKQ"
     gene            3162..3917
                     /locus_tag="VNZ84_05100"
     CDS             3162..3917
                     /locus_tag="VNZ84_05100"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HWU66366.1"
                     /translation="MLGIIGGTGLTALDNLNISKRLIVRTPYGEPSQPLVFGEINGKE
                     VVFLARHGGGHTIPPHAVNYRANIWALHSVGVCDLLSVATVGGIARNLIPGDMVLPNQ
                     ILDYTYGRSNTYHDGVELPVRHIDFTQPYSQAMRERCLKAAADVGYSLVDGGVYACVQ
                     GPRLETAAEIDRYERDGATLVGMTGMPEAALAREMGVSYAAICPVANYAAGRGDSAQA
                     IQFEQVMPLLQQTMDKVRTVIAQYLSDNDCSVA"
     gene            3933..4466
                     /gene="def"
                     /locus_tag="VNZ84_05105"
     CDS             3933..4466
                     /gene="def"
                     /locus_tag="VNZ84_05105"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWU66367.1"
                     /translation="MAVKPVLRMGEPLLNRVADRVTAFNTPELHALIQDMHDTMTHMN
                     GAGIAAPQIGVSLQVVMFGVGANPRYPDAEEVPFTILINPELTPVDQVEEEGWEGCLS
                     VPGMRGLVPRYQHLHYRGFDPQGNIIDRTVSGFHARVAQHECDHLFGVLYPMRIRDLT
                     KFGFNDVLFPGITLPDD"
     gene            4492..4581
                     /locus_tag="VNZ84_05110"
     tRNA            4492..4581
                     /locus_tag="VNZ84_05110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4526..4528,aa:Ser,seq:tga)
     gene            4653..>4830
                     /locus_tag="VNZ84_05115"
     CDS             4653..>4830
                     /locus_tag="VNZ84_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU66368.1"
                     /translation="MTQIIIDHNPSEEKLNELGVRSWNTWDCPPSKFPLDFTATEKAY
                     VLEGEFKVTPQGGDT"
BASE COUNT         1130 a         1185 c         1304 g         1211 t
ORIGIN      
        1 ggcgatggta tttatcggcc gtcaaataat caccttgccg ttttgtgcaa agattctgcc
       61 agtgaataat caccattcat gatggcttgg gcagcggcat gtccgctgcg cacggtgcct
      121 tctatcgttg ccgggtagcg gcctgccaca taatctccgg ctaaaaacag atgtggaatg
      181 ccagtcttga tcggggggcg tgataaatcg ggggtgcaac taaaggtcgc ccgtttttca
      241 gtgatgattt gtgtccagag tggggtggtc gataactgct taccgtacat gtccaaggca
      301 cgattaatct cctcgataca ttgcctgagc aggctctctt tacccatgtt cagtggtgga
      361 tgtgcgctga tgaccacggc cagtaaacca ggttgatcgc agcactgccc ccggtcaaac
      421 acccattggg ccagtccatg acacaatccc atgacgacgt gaggcaggcg gaagccagat
      481 tcaaattgca ggtatatcgt ggtgattggc tggtaatcga atcgcgggat gaccagctca
      541 ctttcaatcg ttttcagttg atgcggcccg acagccacga ctacatcgtc aaaagcgacc
      601 gtactggtcg ccgtcgagac caggcaacct gtgtctaatg gctggatgga ggtgactgcc
      661 tggcgcaggt gtacctctgc ccctaaatct tgaatggcct gcaagagtgg ttgaatcaac
      721 gcagtcgata aatcgccttt aacaatcaga aagtcgctgt cttgacggcg atgctggaaa
      781 ctatcctgca gtacattgag aaatacttgg gcgctggctt ctgcaatcgg agtattgagt
      841 gcccccagac acaagggctc ccacaatgtg ctgattgcga tggctgtttg ctggcctcgt
      901 accagaaggg ctgcaacggt gatgtctttt tctaatctaa acccaatacg ttttaagtct
      961 agcatccagc ggatggcctg ccatttgctg cgccaatcca gtccacgagc agccagtaaa
     1021 ccccataaca aatggagcgg ggcaggcagc catgcaggcg tgactaatga catgcgttgc
     1081 tggctattgt gagtccaggc atgcatatgc aaggagagtg gcagtcgcat gaagacttct
     1141 tcaggcttga gtctagctgc ctgtagcaag ttgagcgttg cgctgtaagc gcctatacac
     1201 aggtgctggc cattatccag tgtagtgttg ccatgagtga ctccgcgcgc gcgtcctcct
     1261 gcctgtggtg ccgcttcaaa cacgattaca tggtggcctt gccgtagtaa cttatgtgcc
     1321 gcagataagc cagccagacc ggcaccaata atggcaattt ttcggcggga aaatgctggc
     1381 gttgccatca cttgcgatac ttaaaccagg cgcccagcgc cagcttcagc tttctaaacg
     1441 gcggcagcat gactttatgg gtgagtacca ggttgggctc aggcttgatt tcctgtagta
     1501 acgcgcggta gatggctgcc atcatcagtc ccggtaattg cggtttgata tctgctttag
     1561 gtagctgatt taatgccttg tcataaaaca gctcggcacg ctcaatctgt ctcagcaaga
     1621 gtgcctgcaa tgctggtgag gcctggctgt tgagtaattg ttgttcagtg acgcctgcgc
     1681 gctccatttc atccaatggg atataaatgc gtccgcgtct tgcatcttca cctacatcgc
     1741 ggatgatatt ggtgagctgg aaggccatgc ccaggtcatg cgcatattgc aatgttgcag
     1801 ggtcgctgta accaaaaatt tgtgcggata gcagccccac cacactggca acacgatagc
     1861 agtacaaggc taattgctca aagtctgcat aacggttgta ttgcgtatcc atttgcatgc
     1921 catcgatgat ttcgatgaag tgtgcttctt tcaggtcgaa ggtcttgatc actggctgca
     1981 atgcctgtgt gactgggtgt tgcggtgtct cgtggaacag cctgtggatc tcctgtttcc
     2041 accaagccag tttggtctgg gcaatctggt aatcggtaca ttcgtcgacc acatcgtcca
     2101 cttcgcggca aaacgcatac aacgcggtca tggcatcgcg tttttcttta ggtaaaaaca
     2161 taaaactcag cgtgaagctg gagccactct cacgggtttt ctgttggcaa tattggtgcg
     2221 gggtcattgt ttaaacaggg ctttcaacaa aatcagcggc cagtcatagg ctttcagggt
     2281 cgggcgatgg tgatatatat caccacggca ggattttaat ttatgcagga tacgttcgcc
     2341 accggcaatc atcattcgca attcaaatcc aatgcggcca ggtaatgcgc ggcccaatgg
     2401 ttttcctgcg agcagcatgc gctcggcacg cagtacgttg tgcacgaaaa aagcctgcca
     2461 tggtgcatct actcgttgct gatgcaagtc ctgctcgctg ataccggcag cttgcatttc
     2521 atcctggcta aggtaaatgc gaccggtttg accatgtttc tgcaggtcta tggcaatgtc
     2581 ctggtagaag ttgatcaatt gcaaagcggt gcatatctgg tcagaccata actggtgttg
     2641 cgcggtatcg ctaccataca gctgcaacat caggcggcct accgggttgg ccgagtaacg
     2701 gcaataatcc agcacctcgt caaagctggc atagcgtgtt ttgaccacat cctgtttgaa
     2761 cgcgtatatc aggtccagca agagctgcac gtccagccga tgcgtcttga ccacttgttg
     2821 cagtgccaga aacaaaggtt gtgatggctg gatatacgcc tgagtcagca ataactcatc
     2881 ctcatacgtc tgcaaggcac gcaatcgatc agcttcactg gcgtcacctt catcggcgat
     2941 atcatctgct tcacgtgcga actggtaaat caccgtgacg gcatgacgta aatgtgctgg
     3001 caacagcaaa gtggcaaccg gaaagttttc gtaatgggtg tgcgccagcg tcaggctgtc
     3061 ttgctgctgg gctggcttgg aaaaggcttg catggcttaa gtatgccata aatccgtgat
     3121 acgcttacgc cttgcatatt aacaagtagt aaagagaact tatgttaggg attattggcg
     3181 gcaccgggct gacggcactg gataatttga atatttctaa acgcctgatt gtgcgcacgc
     3241 cttatggaga accttcgcag ccgctggtat ttggcgagat caatggcaaa gaagtggttt
     3301 ttctggcgcg tcatggcggt ggacatacca ttccgccgca tgccgtcaat taccgcgcca
     3361 atatctgggc actgcattct gtcggcgtgt gtgacttgct ctcagtggca actgtgggcg
     3421 gcattgcccg aaatttaatt cctggcgata tggtgttgcc aaaccagatt ctggattaca
     3481 cctatggccg cagcaatacc tatcatgatg gggtggagtt acccgtgcgc catatcgact
     3541 ttacacaacc ctattcacaa gccatgcgtg aacgctgtct caaggccgca gcagatgtag
     3601 ggtatagcct ggtggatgga ggtgtctatg cctgtgtgca aggccctcga ttggaaacgg
     3661 cggcagagat agaccgatac gagcgtgatg gtgcgacact ggtaggcatg acaggtatgc
     3721 cagaagcggc tttggcacgg gagatgggtg tctcttacgc cgcaatttgc cctgtggcta
     3781 actatgcggc cggacgcggg gatagtgccc aggcaatcca gtttgagcag gtgatgccgt
     3841 tgttgcagca aaccatggac aaggtacgta ccgtgatcgc gcagtatttg tccgataatg
     3901 attgttctgt agcttaaacc aaaggaataa gcatggcagt gaaaccggta ttacgcatgg
     3961 gtgaaccact gttaaaccgc gtggcagacc gggtgactgc gtttaatacg cctgaattgc
     4021 atgccttgat ccaggatatg catgacacca tgacccacat gaatggcgca gggattgctg
     4081 cgccacagat cggggtcagc ctgcaggtgg tgatgtttgg cgtgggagca aacccacgtt
     4141 acccagatgc tgaagaagtg cctttcacta ttctgattaa tcccgagttg acgccggtgg
     4201 accaggtaga agaagagggc tgggaagggt gcctgtctgt gcctggcatg cgtggcctgg
     4261 ttccacgcta ccagcatttg cattaccggg gttttgaccc acaaggaaat atcattgacc
     4321 gcactgtgag tggctttcat gcacgcgttg cccagcatga gtgtgaccat ttatttgggg
     4381 tgctttaccc catgcgcatt cgcgacctca caaaatttgg ctttaatgat gtactttttc
     4441 ctggaataac gcttccagat gactaattgt ttgctataat gcgcgctttc gggaagagtg
     4501 gcagagcggt ttaatgcaac agtcttgaaa actgtcgagg gttcacgccc tccgtgagtt
     4561 cgaatctcac ctcttccgcc agaacatgaa tgccgcagga atgcggcatt ttcatttgca
     4621 ggttttattc attacatatt taaggaaacg tcatgacaca aatcatcatc gaccataacc
     4681 cgtctgaaga gaaattgaac gaactgggcg taagaagctg gaacacctgg gattgcccac
     4741 cttccaaatt ccctctggac ttcactgcca ctgaaaaagc ctacgtgtta gaaggcgaat
     4801 tcaaagtgac tccacaaggt ggtgacactg
//
LOCUS       DATMSK010000151         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_316929,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000151 DATMSK010000000
VERSION     DATMSK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316929"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1369
                     /locus_tag="VNZ84_05120"
     CDS             2..1369
                     /locus_tag="VNZ84_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HWU66369.1"
                     /translation="MLGVSIVLAVVVFNWWQEKKYRRDVEYRFSHTRGDVLDNDSSSG
                     KTKNSAQKAAAEKNTVSGDVEGWMSEPRVRHSPKFEPDFSVNESVTAKKTAGDKSGLN
                     LNKPSGEVRVATPKNEPSIPAPTVAKAPVAEIPNNQASHQPTPDTLHVEVEENEQDPF
                     ALEEPIVASNLATDAVLPAAVHTAMDLVGLIDAPLGVMLAELEDMQAEVRTFPQHATL
                     WAQNSISGNWQDVLGLDAKGMQDQTPASQVVCSLQLADRGGPVDEGTLKRFQKSMETL
                     ARALGLSVSWQGGYEPSQQAQSIDAFCIEVDKAVEFHVLASQGMFHATKLRGLAEACG
                     MQLSDDGRFELFNSEGQLEFSVRNYEGKPFSADMLKSAVMTGVTFQLDIPTTPHSLRV
                     FDHMVEVASNMAASLSGSLIDVNRKPIGDVQLDKIRQQLKNISTAMAVQGIIPGSSHA
                     KRLFS"
     gene            1372..3447
                     /gene="ligA"
                     /locus_tag="VNZ84_05125"
     CDS             1372..3447
                     /gene="ligA"
                     /locus_tag="VNZ84_05125"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWU66370.1"
                     /translation="MSAQEVALTLQQLKQRLAEYDYHYYVLDAPLVTDNEYDGLYRQL
                     VEIEQQFPELVTPDSPSQRVGGQALSAFASVQHRQAMLSLNNAFSDDELEAFDRRVRE
                     GAGVSVVEYAVEPKFDGLAITLTYEQGFFVQGATRGDGYTGENVTHNLKTIRAIPSRL
                     NMTNPPALLEVRGEVLMLKKDFEKLNQQQVAVGGKLFANPRNAAAGSLRQLDSNITAK
                     RPLHFFAYGLGASDGVPELHSHAEAMQYLADLRFPVSVLRGVKTGVQGLRDYYAEIGE
                     KRKDLPFDIDGVVYKVNDFDMQETLGFVSRAPRWAVAHKFPAEEATTVVEDITVQVGR
                     TGAITPVARLKPVFVGGVTVTNATLHNEDELRRKNILIGDTVVVRRAGDVIPEVVLVK
                     PELRPGNARTFVMPTACPECGSHVVRLEDEAVARCTGGLICPAQRKQAITHFASRRAM
                     DIEGLGEKLVDQLVARELIHHLDDIYRLDLPVLAGLDRMADKSTQNVLAAIEKSKQAT
                     LPRFIYALGIRNVGEATAKDLAQHFGSLEALMEADIETLMQVNDVGPVVAEAVYQFFL
                     EPHNREVIAAMRALGVHWADIAKTKSSAAFAGKTFVLTGTLPTLKRDQAQAMIEAAGG
                     KVSGSVSAKTSFVVAGTEAGSKLEKAQQLNIPILDETALLEMLSFPANSVEVENPAQT
                     SHSKQPGLF"
     gene            3392..4327
                     /locus_tag="VNZ84_05130"
     CDS             3392..4327
                     /locus_tag="VNZ84_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWU66371.1"
                     /translation="MKSKTQLRHPIQNNLVYFSMNSKVTLALLINLLILKESVAKDNA
                     LEACDVALKNRRYTQAIQLAEQHLSQAEFWLCKGRAQYGLDQHEAAQASFKQAIDLHP
                     QGLDLISAYILLGNAQLAAKAHAVSIESYQKALKFSEEQNMRRYTRIAHNLIGEAYFE
                     MGQYAESLKAFETAEKLALNDDERADSYIHEAMAYQQLQQIDKAVEYQLKGVLMLRKS
                     GTPDQYAEASLTLGQLFVAKKDFVGADKTYQRLMDFARENGGSFYEAKTAIYWAEAKR
                     TQGDAGGADQLIKQAEMIALQLKDPELDALLAKGK"
BASE COUNT         1175 a          991 c         1163 g         1119 t
ORIGIN      
        1 gttgctaggg gtcagcatcg ttctggcagt ggtagtgttt aactggtggc aggaaaaaaa
       61 atatcgtaga gacgttgagt accgcttcag ccatacacgt ggcgacgtat tggataacga
      121 ttcttcttct ggcaaaacca aaaattcagc ccagaaagcg gctgctgaaa aaaacaccgt
      181 ttcaggagat gttgaaggct ggatgtccga gcctcgtgta cggcactctc ccaaatttga
      241 gccagatttt tctgttaatg aatctgtcac cgcaaaaaaa actgcgggtg ataaaagtgg
      301 attaaatctt aacaagccat ccggtgaagt acgggtagcc accccaaaaa atgagccttc
      361 tattccagcg ccaacagtgg ccaaggctcc tgtagcagaa attcccaata atcaagcgag
      421 tcatcagccg acgccagata cgctacatgt tgaggttgaa gagaacgaac aggatccatt
      481 cgccttagaa gagcccattg tggcgtcgaa tttagcgaca gatgcagtgt tgcctgctgc
      541 ggtacataca gccatggatt tggttgggct gattgatgcg ccgttaggtg tcatgttggc
      601 tgagttggag gacatgcagg ctgaagtaag aacctttcca caacatgcta ccctgtgggc
      661 acaaaattca atctcaggca attggcaaga cgtgctcggc ctggatgcta aaggcatgca
      721 ggaccaaaca cctgcaagcc aagtagtgtg tagtttgcaa ttggctgacc gtggcgggcc
      781 tgttgatgag ggaacactca aacgtttcca gaaatctatg gaaaccctgg cacgagcact
      841 tggtttgagt gttagctggc agggcggcta cgagccttca caacaggcac aatctatcga
      901 tgcattctgt attgaggtgg acaaggcggt tgaatttcat gtgcttgcta gccaggggat
      961 gtttcatgcg acaaaattgc gtggactggc tgaggcttgt ggtatgcagt tgagcgatga
     1021 tggccgtttc gagttgttta actctgaagg tcagctggag ttttctgtac gaaattatga
     1081 aggtaagcca ttttctgccg atatgctgaa atctgccgtg atgacgggtg taacttttca
     1141 acttgatatt cctaccacgc cgcatagcct gcgggtgttt gaccatatgg ttgaagttgc
     1201 gagcaatatg gcagccagct tgagcggtag cctgattgac gtgaaccgta aaccgatcgg
     1261 tgacgtgcaa ttggataaaa tccgtcagca attgaaaaat atctccactg ccatggccgt
     1321 acaaggaatc attcctggta gcagccacgc taaacgtctg ttctcttaat aatgtctgcc
     1381 caagaagttg cgctgacttt acagcagctt aagcagcgcc tcgccgaata tgactatcat
     1441 tattacgtgc tggatgcacc gttagtcact gacaatgaat acgatggcct ttaccgtcaa
     1501 ttagttgaga ttgagcagca attccctgag cttgttacgc cggactcgcc tagccagcgg
     1561 gtaggcgggc aagcactttc cgcctttgcc agcgtccagc atcgccaggc aatgctgtct
     1621 ctcaataatg ctttcagtga tgatgagcta gaggcttttg accgtcgtgt acgggaggga
     1681 gctggcgtct ctgtggttga gtatgccgtt gaacccaagt ttgatggttt ggcgatcacc
     1741 ctaacctatg agcagggttt ttttgtgcaa ggtgccacac gcggtgatgg atataccggt
     1801 gaaaatgtca cgcataacct taaaaccatc cgggcgattc ctagccgcct caatatgacc
     1861 aatccaccag cgttgcttga agtccgcggg gaagtgctca tgctgaaaaa ggactttgaa
     1921 aagcttaacc agcaacaggt ggcggttggc ggcaaattat ttgccaatcc acgcaatgcg
     1981 gctgccggta gcctgcgcca gctcgactct aatatcacag ctaaacgccc cttacatttt
     2041 tttgcttatg gcttaggtgc cagtgatggt gtcccagaac tgcactctca tgccgaagcc
     2101 atgcaatatc tggctgattt acgcttccct gtctctgtgc tgcgaggggt gaaaaccggc
     2161 gtgcaggggt tacgtgatta ttacgcggaa attggtgaaa aacgtaaaga cttgccattt
     2221 gatattgatg gcgtcgttta taaggtgaac gattttgaca tgcaggaaac tcttggattt
     2281 gtgtcgcgtg cacctcgctg ggcagtcgcc cataaattcc cggccgaaga agcgacgaca
     2341 gtggtcgagg atatcactgt acaagttggc cgtaccggtg ccattacgcc cgtggccagg
     2401 ctgaagcctg tgttcgttgg tggtgtgact gtcaccaatg ctacgctgca taatgaggat
     2461 gagttacgcc gtaaaaacat tttgattggc gatacggtgg tggtgaggcg ggcaggggat
     2521 gtcattcctg aagttgtgct ggtaaagcct gaattgcgcc cgggcaatgc gcgtactttt
     2581 gtcatgccaa cggcatgtcc tgaatgcggc tcccatgtgg ttcgcctgga ggatgaagct
     2641 gttgcacgct gcacaggggg gttgatttgc cctgcgcagc gcaagcaggc aattacgcat
     2701 tttgcttctc gccgtgccat ggatattgaa ggtttgggtg aaaaactggt ggatcagcta
     2761 gtcgcgcgtg agttgattca ccatttagat gatatttacc gcctcgattt gcctgttttg
     2821 gctggcttgg accgcatggc cgacaagtca acacagaatg tactcgccgc tattgagaaa
     2881 agtaagcaag cgacattgcc acgctttatc tatgcgcttg gtatccgaaa tgtaggcgag
     2941 gcgacagcca aagacctggc gcagcatttt ggcagcctgg aagctttgat ggaagccgat
     3001 atcgaaacac tgatgcaggt gaacgatgtt ggccccgtgg tagcagaggc tgtttaccag
     3061 tttttcctgg aaccacacaa ccgcgaagtc attgcagcca tgcgggcctt gggtgtgcac
     3121 tgggccgata ttgctaaaac caagtcatct gcagcttttg caggtaaaac atttgtcctc
     3181 actggcacat tacccacgct caagcgtgat caggcacaag ccatgattga agcggcgggc
     3241 ggcaaggtca gtggtagtgt ctcggcaaaa accagtttcg tggtggccgg tactgaggct
     3301 ggaagtaaac tggaaaaagc tcagcagctc aatattccca ttttggacga gacagctttg
     3361 ctggagatgt tgtcattccc tgcgaatagc gttgaagtcg aaaacccagc tcagacatcc
     3421 cattcaaaac aacctggttt attttagtat gaattcaaaa gttacattag cattgttaat
     3481 taatttattg attttaaaag aatcagtcgc caaggataat gcactcgagg cctgcgacgt
     3541 tgcgctgaaa aatcgacgct atactcaagc gattcaactc gccgagcaac atctcagcca
     3601 ggcagagttt tggttatgca aagggcgggc gcagtatggt ttagatcagc atgaggctgc
     3661 acaagcaagc tttaagcagg caattgactt gcatccccaa gggctggatc tgatttctgc
     3721 ctacatattg cttggaaacg ctcagcttgc tgcaaaagcg catgcagtct caattgaaag
     3781 ttaccagaag gcattaaagt tcagtgaaga gcagaatatg cgtcgttata cacgtatcgc
     3841 acacaacctg ataggtgaag cttactttga aatggggcaa tatgcagaat cactcaaagc
     3901 ctttgaaacc gctgaaaaac tggctttgaa tgatgatgag cgtgctgaca gctatattca
     3961 cgaagcgatg gcctaccaac aattgcagca aattgacaag gcagttgaat accaactcaa
     4021 aggtgtcctg atgctgcgta aatctggcac tccagatcaa tatgctgaag caagcctcac
     4081 attaggccaa ctgtttgtgg ctaagaaaga ctttgttggt gcagacaaaa cgtaccagcg
     4141 cctaatggat tttgcacgag aaaatggagg ctcattttat gaggcaaaaa cagctatcta
     4201 ttgggcagaa gccaaacgca cacaaggcga tgctggaggt gctgatcaat taattaaaca
     4261 ggcagaaatg attgcattgc agttaaaaga tcctgaactg gatgctttac ttgcaaaagg
     4321 caagtaaagc tatcttttag acttagtaca caaggataga tcgtaattac aaaaccgata
     4381 aaaactctgc gttcaaaagg cagagttttt gtttaatctt ttatttaaca taatatacat
     4441 tatgcgaa
//
LOCUS       DATMSK010000152         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_246357,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000152 DATMSK010000000
VERSION     DATMSK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246357"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VNZ84_05135"
     CDS             <1..541
                     /locus_tag="VNZ84_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HWU66372.1"
                     /translation="LENSVMAAERAAVLKKIGKANIGSIVVVEDTPPQTILPKKEFYS
                     NWQQKALAALQSLLKIQRSTGSHDLDSCFAEFPYFEVIFELEPNGVQRYANWINPNMY
                     GKIKAGGAGIDRSQQAYYQKVVETHAPYISTIYLSTATEDFCLTVSLPILNEDGSLKS
                     ILVADINIAAMAVLSSARH"
     gene            complement(558..1202)
                     /locus_tag="VNZ84_05140"
     CDS             complement(558..1202)
                     /locus_tag="VNZ84_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWU66373.1"
                     /translation="MQAVLFDLDGTLVDTAPDLGYALNLQRQRHGLNFLPEETIRPYA
                     SHGSRGLLEVGFGLLPTDSHFEAMRTEYLDLYTQVMTRQPMLFEGMAETLQAIEQGGL
                     RWGIVTNKPRRFTQPMMAHMGLDVRAGAVISGDDASQPKPSPQTLFLACERMKIDPKA
                     VVYVGDAERDVQAGNAAGMKTLVALFGYLSANDKPKEWGAYGCIQQPKDLLAYL"
     gene            complement(1215..1913)
                     /gene="ubiG"
                     /locus_tag="VNZ84_05145"
     CDS             complement(1215..1913)
                     /gene="ubiG"
                     /locus_tag="VNZ84_05145"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HWU66374.1"
                     /translation="MMNVEAAEVNKFAELAHQWWDTQGVFKPLHQLNPLRLNYIDSRA
                     QLAGKQILDVGCGGGILSESMAQRGAQVTGIDLAEKSLQVAQLHALEAGVQLEYRCVA
                     VEALAQEKPQAFDVVTCMEMLEHVPDPASVVRACAALVKPGGHVFFSTLNRNAKAYLM
                     AVVGAEYVLNLLPKGTHDYSKFIKPSELAAWMRQSELELQHQTGVTYHPLNKQYALTV
                     DTSVNYMLHAIKEV"
     gene            complement(1945..>3239)
                     /locus_tag="VNZ84_05150"
     CDS             complement(1945..>3239)
                     /locus_tag="VNZ84_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HWU66375.1"
                     /translation="WVIPVDASHTLLEQQAILVEEGEIKAIIPKAEIPAWAADWPVVD
                     CSQHALIPGLINLHAHSSMNLLKGYADDYALMPWLNEHIWPAEKKWVSRSFVQDGSLL
                     ACAEMLSGGITTFNDMYFFPQATIEAVKRAGMRAHIGLTVMEFPTNYAATANDYIRLG
                     TEVRDQYKSDEHISFSFAPHAPYTVSNQTFERIATLSEQLNLGIHTHLHETQAEIQQS
                     LEQFGVRPLQRLLGLGVIGPATVLAHGVHLETSELDTLSIQGAHIAHCPSSNLKLASG
                     IAPVAAALRTGINIGLGTDGAASNNRIDLWQEMRTAALLAKVADQNAEAVPAYQALEM
                     ATLYGARALGLQDKVGSIEVGKQADLVAVKLDDLNSLPCFDPVSHLVYVAGREQVTHT
                     WVSGRLCYQRHAEGRSEFTGMETEELRSIALQWQLKLQ"
BASE COUNT          850 a          876 c          721 g          792 t
ORIGIN      
        1 actggaaaat agcgtgatgg ccgccgaacg ggccgccgta cttaaaaaga taggcaaggc
       61 aaatatcggg agtatcgtgg tcgtagagga cacgccacca caaacaatct tgcctaaaaa
      121 agagttttat tctaactggc aacaaaaagc gctcgctgcc ttgcagtcat tgcttaaaat
      181 ccagcgttca acaggttcac acgatctgga cagctgcttt gcagaattcc cttattttga
      241 ggtaattttc gagctggagc ccaacggagt tcaacgctac gccaattgga tcaacccgaa
      301 tatgtacggc aagatcaaag cgggcggtgc aggcatagac cgtagccagc aggcgtatta
      361 ccaaaaagtg gtggagacac atgctccgta catctcgacg atttacctgt ccacggccac
      421 agaagatttc tgcctcacgg tatcgttgcc aatcctgaat gaggacggtt cgcttaaaag
      481 cattttagtc gcggatatta atattgccgc gatggccgta ctaagtagcg cgcgtcatta
      541 atgaaacgtt taagaaatta cagataggca agcaggtcct ttggttgctg aatacaacca
      601 taggcgcccc actcttttgg tttgtcattg gcagataaat agccaaacaa ggccaccagt
      661 gttttcatac ccgcggcatt acctgcttgc acgtcgcgtt cggcatcgcc cacatatacc
      721 actgctttcg ggtctatctt catacgctcg cacgcaagaa acagggtttg tggtgaaggc
      781 ttgggttgtg acgcatcatc accactgatg accgctcccg cacggacatc cagccccata
      841 tgcgccatca taggctgcgt aaagcgacgt ggcttattgg tcacgatacc ccaacgtaac
      901 ccaccttgct caatcgcctg caaggtttct gccattccct caaacaacat gggctggcgt
      961 gtcatcacct gcgtatacaa atccaggtac tcagtacgca tggcctcaaa atgactgtct
     1021 gtgggcagca aaccaaaacc aacttctagc aatccacgtg aaccatgaga ggcataaggg
     1081 cgaatagtct cctctggcag gaaattgaga ccatgtcgct ggcgttgcaa attcagggca
     1141 taccccaaat ccggcgccgt atcgactaag gtcccgtcca aatcaaacaa aacagcctgc
     1201 attcaacttc cttgttaaac ctctttaatg gcatgcagca tgtaattcac actggtatcc
     1261 acggttaacg catactgttt gttcaaagga tgataagtca ccccggtttg atgctgcagt
     1321 tccagctcag actgacgcat ccacgctgcc agctccgatg gcttgataaa tttgctgtaa
     1381 tcatgggtgc cttttggcaa caaattaagc acatattcgg caccgacgac agccattaaa
     1441 taggctttgg cattacgatt cagagtagag aaaaaaacat ggccgccagg tttcactaaa
     1501 gcggcgcaag cacgcacgac acttgcaggg tcaggcacat gctccagcat ttccatgcag
     1561 gtcacaacat caaatgcctg aggtttttcc tgcgccaacg cctccactgc aacgcaacgg
     1621 tattccagtt ggacaccagc ctccaatgca tgtaattgcg ctacttgcag cgacttttcc
     1681 gcaaggtcta ttcctgtcac ctgtgctccg cgttgcgcca tggactcact caaaatgcca
     1741 ccaccacagc ccacatcgag tatctgtttc ccggccagct gcgcacgact atcaatataa
     1801 ttcaagcgca ggggatttaa ttgatgtagg ggtttaaaga cgccttgcgt atcccaccat
     1861 tgatgcgcca gctcggcaaa cttgtttacc tctgcagctt ctacattcat catatcgttc
     1921 cttctgtatc ctgtgtgcat tagactattg caatttgagc tgccactgca acgcaataga
     1981 ccgtaactct tctgtttcca taccagtaaa ttcgcttcga ccctctgcat gccgctgata
     2041 gcataaccgg ccactcaccc atgtgtgggt aacctgctca cgcccagcca cgtaaaccag
     2101 atgtgaaacg ggatcaaagc atggcagact gtttaaatca tccagtttga ctgccaccaa
     2161 atctgcctgt ttaccgacct caatactgcc aaccttgtct tgcaaaccta atgcacgtgc
     2221 cccatacagg gttgccattt ccaatgcctg gtaagcagga acagcctctg cattttgatc
     2281 tgcaacctta gccagtaatg ccgcagtgcg catttcctgc caaagatcaa tgcgattatt
     2341 acttgcggca ccatccgtgc ccaagccaat attaataccg gtccttaaag cagcagcaac
     2401 cggtgcaatt ccactggcca gctttaaatt tgaagatggg caatgggcaa tatgtgcccc
     2461 ctggatagac aatgtgtcca gttcggacgt ttcaaggtga acaccgtgcg ccaggacagt
     2521 cgctggacca atcacgccca agcctaataa gcgctgtagt ggacggaccc caaattgctc
     2581 caggctttgt tgaatttcag cctgtgtttc gtgcaaatgg gtgtgaatac ccaggtttaa
     2641 ttgctcggat agtgttgcaa tacgctcaaa ggtttgatta cttacggtat aaggtgcatg
     2701 tggcgcaaat gaaaagctga tatgctcatc agacttatac tgatctctta cttcggtgcc
     2761 caaacggatg tagtcgttgg cggtggcagc ataattggtt ggaaactcca tcacagtcaa
     2821 gccaatatgc gcacgcatac ctgcacgttt aacagcctca atcgtcgctt gagggaaaaa
     2881 atacatatca ttgaatgtgg tgatgccacc gcttaacatt tccgcacatg ccagcaaact
     2941 gccatcctgt acaaaagacc tggaaaccca tttcttttct gcgggccaga tatgttcatt
     3001 caaccatggc atcagcgcat aatcgtccgc atacccttta agcaagttca tactgctatg
     3061 tgcatgcagg ttaataagtc ccgggatcaa tgcatgctgg gaacaatcaa ctacaggcca
     3121 atcagcggcc catgcgggaa tctcggcttt ggggataatc gctttaatct ccccctcttc
     3181 cacgaggatc gcctgttgct ctaataatgt atggctcgcg tctacgggaa tcacccagc
//
LOCUS       DATMSK010000153         6592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_340159,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000153 DATMSK010000000
VERSION     DATMSK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340159"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="VNZ84_05155"
     CDS             <1..1770
                     /locus_tag="VNZ84_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66376.1"
                     /translation="RSGPSYPEVDTSEIESTLSIKPPLKSATPTLLINLAVHEAPVQE
                     VLLAIARESGLNIDVHPGLRGKVSLHAVQQSVPSILARMAKQLDMYYEWSNGVWFVTP
                     DMPVIRSYKLNCVNMSRDTTGYIGVASEINSAGRAATSAGNNSVAQVSAAQGGSSNSS
                     RTAVTSQSRYHLWESIVENIQALLDETDKAGNARDEVVGSVSSALQLSDQTVQSPELV
                     QRAPALEQTGHAEKMNQAHVRWRASRLITNPETGILTIRASQSQQAQVRTYLESVMQV
                     AARQVLIEATIVEVELSKSFQMGIDWSRLSNSAQQRGWQFAQSLGSEAAQFNRSSGSW
                     ESNNSKNALGQNNGSVAFMLGYFNPFSRLGNLSASVTLLEQFGKTRVISSPKLMVLNN
                     QTAILKVVDNLVYFTIQSQISQSSSNSGSNLQSVTTTANTVPVGLVMAVTAQINSHQR
                     VNLNVRPTVSKVLRYIEDPNPQLGSGSARIVSSIPEIQVREMESVLNIASGNIAVLGG
                     LMQEDQREFTDRVPGLSRLPMIGQVFTGKNQAAKKTELIVFLRPTVIGLADVRSGDLQ
                     SFQPLLPEALPADFEINVGGEPS"
     gene            1767..2492
                     /locus_tag="VNZ84_05160"
     CDS             1767..2492
                     /locus_tag="VNZ84_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66377.1"
                     /translation="MKLAQLVASFMPASRQLRRSQPYKGALVNEKDAYFERRLIACLW
                     IGIALLAYLVCRQAYYFCLENEKAYPFITQKSALHRPNIPALKTTKHSDILPAMQEIK
                     IGRESSLFKIGQVTAPQRPSEVSQYPREEKHRSKIGPQKTNSHSHVWQLEVKRLSEAN
                     MAYQLADARDQLQAGHLELARQSFEQILEKDPHQVVALAGMLVVISQRGDISQRENYL
                     QRLRQEIPDFMPDDDLFLLQVAD"
     gene            2534..4213
                     /locus_tag="VNZ84_05165"
     CDS             2534..4213
                     /locus_tag="VNZ84_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWU66378.1"
                     /translation="MLEKGMITADQLDIALIEQHKSHLLLGQQLVRLHFVTDAIIRDV
                     LAQHQAQESIDLQQVIPDHEALAMVPEIFARQHKLLPLDLSADRSSMRIALSGPTHLL
                     VLDELRHMLGGNMQLEVVLATEAHLEQALDKFYGHSLSLDSILQDIEQEHTTGLPSLQ
                     QESRPVIRLVNALLIDAVKQGASDIHFEPEQAFLRIRYRIDGVLWQIRCLHGRYWPAI
                     CVRLKILAGMDIAEQRLPQDGRVDLFLCGRQIDFRGSSHPTLYGENIVLRVLDRDKAI
                     IALDAMGLGAPKLSQVKAMLAKPEGLLILTGPTGSGKTTTLYSMLSHLNHEHVNIMTL
                     EDPVEYPHPLMRQTSINAANKVDFSNGIRAILRQDPDIILIGEVRDESTAAMAMRAAM
                     TGHLVMTTLHTNSAAAAFARLQDLGVSPHMMAGCLVGVIAQRLLRRLCFYCKRNLEVD
                     HELAQWLGQVTGVEPSALQLFEAEGCVHCRMSGYRGRLVIMEVLAMHRDMEVMLTQSY
                     SMPSLQEQARQHGYTTLAEEGLKVVVSGETSLQELRRVVDLSSLGSRIGEI"
     gene            4210..5409
                     /locus_tag="VNZ84_05170"
     CDS             4210..5409
                     /locus_tag="VNZ84_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWU66379.1"
                     /translation="MTYYRYTAIDQYGRYQRGCLDAPTEQSLVAYLQQRELELLTFKL
                     GRPLLRWQPRVNISALILFCVQMEQLLQAGVPLLQAISEVAEQSEHKGLQAALLTVQR
                     ELEAGKLLSQALLAQPKIFPPLFGQLIDAGERTGQLPEIFGHLNRTLTWQAELVAQIR
                     RGLTYPAVLCIMVAVAAYVMLTFLVPQMASFLQSLGQDLPWSTRLLLGLSNGLMRYGG
                     WLLFAITGLTGAVVLAVRHSPRYALLLDQCLLKLPVLGRLLHQLVLARLCRFLGLMYQ
                     TGIPLLQALEWCQPLLQQRFIALAVAEIHQRVLAGESLTSSFRATQQFPPLMLRMLSV
                     GETTGALDRMLLQLAVFYDREVQSQLQTLLRLLEPALTIALGCMLLFLMAAVMLPVYD
                     SFSSLRY"
     gene            5457..>6592
                     /locus_tag="VNZ84_05175"
     CDS             5457..>6592
                     /locus_tag="VNZ84_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66380.1"
                     /translation="MRVIHCVGQNVLALREFGFTDDSDVALLRWCQQFPQASVSFLSN
                     LADEHYHVETLPHVRGAAQRQLLLRKLAAWPFAQGMYTAFRLSSVQNLRKEDRFLFVA
                     LFCPPLLAWLSGQQPRSLRIQGIYTQALTLPYCLPALPKGVSHRLCIQCTPHHVRISY
                     LQQHRLFLSRFIPMSQEALPDLEERFNRIAQEANQIRMTLIQQRWVAEADILQIVWLG
                     QEPADTRGLKKHLPAHVLWVFMPTVELSRQSGCSPVLKGIDVMDWMSIQPILRGRALP
                     NLAPQEALLQSSIIRIKRYVHQAGAAMVALMLLAGWIAVQATQSDFLKIKQLNHHLQA
                     LQPVKPVNGLSESQWPQVSVLTQTVQKLEASLRSPADALQALQNT"
BASE COUNT         1590 a         1512 c         1811 g         1679 t
ORIGIN      
        1 cgatctggcc cttcttatcc tgaggtggac acctccgaga ttgagtctac tctttcaatc
       61 aagccaccct taaagtcagc gacgcccacc ttactgatta atttggccgt acatgaagca
      121 cccgtgcagg aagttttgtt agcgatcgca cgtgaaagtg gtttgaatat tgatgtacat
      181 cctggcctgc gcggtaaggt cagccttcat gccgtccagc aatctgtccc atccattctg
      241 gcccgcatgg caaaacagct ggatatgtat tatgagtggt cgaatggtgt atggtttgtc
      301 acgccagata tgccggtcat tcgtagctat aagttgaact gtgtgaatat gagccgcgat
      361 accactggtt atatcggtgt cgctagcgaa attaacagcg ctggtagagc cgccaccagt
      421 gccgggaata atagtgtagc ccaagtctct gctgcacaag gcgggtcaag caatagctct
      481 agaacagccg ttacctcaca atcacgttat cacttatggg agagtattgt tgagaatata
      541 caggcattac tggatgaaac agacaaggca gggaatgcgc gtgatgaggt ggttgggagt
      601 gtttcctccg cactgcagtt gtccgatcag acggttcagt caccagagtt agtgcagagg
      661 gcgcccgcac tggaacagac tggtcatgca gaaaaaatga accaggcgca tgtgcgttgg
      721 cgggcttcac ggctgattac caatcctgaa acagggattt tgacgatcag ggcttcccag
      781 agtcagcagg cacaagtccg gacctatctg gagtcggtaa tgcaagtggc ggctaggcaa
      841 gtgttgattg aagcaaccat tgtcgaagtc gaactcagta aaagtttcca gatggggata
      901 gactggagca gattatcgaa ctctgcccag cagcggggct ggcagtttgc acaatcgctt
      961 gggagcgaag cggcgcaatt taaccgttca agcggcagtt gggaaagtaa taacagtaag
     1021 aatgcactgg ggcaaaataa cggcagtgtt gcatttatgt tgggttactt taacccattc
     1081 agccgcttgg gtaatcttag tgcaagcgtc acgctacttg agcagtttgg caaaacacga
     1141 gtgatatcca gccctaaatt gatggtgctg aataaccaga ctgcgatact caaggtggtt
     1201 gacaatctgg tgtacttcac gattcagtcg caaatttcac aaagtagtag taatagtggc
     1261 agcaacctac agtcggtgac gacgacggca aataccgttc cagtaggcct ggtgatggcg
     1321 gtcactgccc aaatcaacag tcatcaacgc gttaatttga atgtgcggcc caccgtttcc
     1381 aaagtattgc gctatatcga agatcctaat cctcagctcg gaagtggtag cgcccgtatc
     1441 gtcagcagta ttccggaaat acaggtgcgg gaaatggagt cagtgctgaa tattgcgagt
     1501 ggtaatattg cggtgcttgg cgggttaatg caagaagatc agcgagagtt cactgaccgg
     1561 gtgcctggct tgtccaggct accaatgatt ggtcaggtat tcaccggtaa aaaccaggca
     1621 gcgaaaaaaa cagagttgat tgttttcctg cgaccaaccg tcatcgggct tgcggatgtg
     1681 aggtctggcg acttgcagtc gtttcaaccc cttttgccgg aagcattgcc cgcagatttt
     1741 gagatcaatg tgggcgggga gccatcatga aacttgcaca gctagtcgcg agtttcatgc
     1801 ctgcgagcag gcagttgcgc agatcgcaac catacaaggg agcattggta aacgaaaaag
     1861 atgcgtactt tgaaagaaga ctgattgcgt gtttatggat tggaatcgcc ttattagctt
     1921 acctggtttg tcgtcaggca tattattttt gtctcgaaaa tgaaaaagct tatcccttca
     1981 tcacccagaa aagtgcttta cacagaccga atatacctgc tctgaaaaca accaagcaca
     2041 gcgatatctt gcctgcgatg caagaaataa aaattggcag ggagtcgagt ctgtttaaaa
     2101 tcgggcaagt caccgcgcct cagcgtccct cggaggtgag ccagtatcct cgagaagaaa
     2161 agcatcgctc taagataggt cctcaaaaaa caaatagcca ttctcacgtt tggcagttgg
     2221 aagtcaaacg cttgagcgag gccaatatgg cgtatcaact tgctgatgcc agagatcagt
     2281 tacaggcagg gcatttggag ttggcacgtc agtcttttga gcaaatttta gagaaagacc
     2341 cacaccaagt ggtcgcttta gcgggcatgt tagtcgtgat cagtcagcgg ggtgatatct
     2401 ctcaacggga aaattacctg cagcgattgc gtcaggaaat tccagacttt atgcctgatg
     2461 acgatctgtt tttgttgcag gtggcagatt gaatacgccg ctagcttcag ttgtcccgcg
     2521 cttgggagag tggatgctgg aaaaaggcat gattacggct gatcagcttg atattgcctt
     2581 gattgagcag cataagagcc atctattgct agggcagcag ttggtccggt tgcattttgt
     2641 gacagatgcc atcatccgtg atgtgttggc gcagcatcag gcccaggaaa gcatagattt
     2701 acaacaagtc atcccggatc atgaggcgct ggcaatggtg ccggagattt ttgccaggca
     2761 gcacaaattg ttgccgctag acctgtcagc agataggtcc agcatgcgta ttgccttatc
     2821 gggcccaacg cacttgttgg tgctggacga actacggcat atgctggggg gaaacatgca
     2881 acttgaagtt gtcctagcta ccgaagcaca tctggagcaa gcgctggaca agttttacgg
     2941 ccatagtttg tcgctagata gcattctgca ggacatcgag caggaacaca ccacaggatt
     3001 gccttcactt caacaagagt cacggccggt aatcaggctg gtcaatgcgt tgcttattga
     3061 tgccgtgaag caaggggcat ctgatatcca ctttgagcct gagcaggcgt ttttgcgtat
     3121 ccgctatcgt attgatggcg tgctgtggca gatcagatgt ttgcatggtc gttactggcc
     3181 tgcgatatgt gtacgactca agatcttggc cggcatggat attgccgagc aacggctgcc
     3241 gcaagatggc cgggttgatc tgtttttatg tgggcggcaa attgatttta gggggtctag
     3301 ccatccaacc ttgtatggtg aaaacattgt gctgcgtgtg ttggaccgtg ataaggcaat
     3361 tattgcgctg gatgcgatgg gattgggggc accgaaactc agtcaggtca aagccatgct
     3421 tgccaaaccg gaaggcttgc tgatcctgac agggcctact ggcagcggta aaacaacgac
     3481 tttgtattcc atgttaagcc atctcaacca cgagcacgtc aacatcatga cgctcgaaga
     3541 tcccgtcgaa tatccacatc ccctcatgcg gcaaacatcc atcaatgccg ccaataaagt
     3601 ggattttagt aatggaatcc gcgccattct gcggcaagat cccgacatca tcctgattgg
     3661 tgaggtgcgg gacgaatcga cggctgccat ggcaatgcgc gcagcaatga caggtcactt
     3721 ggtcatgacg accttgcata ccaatagtgc ggctgctgct tttgcacggt tgcaagactt
     3781 gggggtgagt cctcatatga tggcgggttg cctggtggga gtcatcgcac agcgcttgct
     3841 aaggcgcttg tgtttctact gtaagcgtaa ccttgaggtg gatcacgagc tggcgcaatg
     3901 gttagggcaa gttacaggcg tagagccatc cgcgcttcag ttatttgaag ccgaggggtg
     3961 cgtacactgt cgtatgagtg gatatcgcgg caggcttgtg attatggagg tgttggccat
     4021 gcaccgcgat atggaggtaa tgttgacgca atcatactcc atgccaagcc tgcaagaaca
     4081 ggcaaggcaa catggatata cgacgctggc agaagagggg ttgaaggtcg tcgtcagcgg
     4141 tgaaaccagt ttgcaggagt tgcgccgggt ggtcgatttg agctcgcttg gcagtcgtat
     4201 cggtgagata tgacttacta tcgctatact gcgattgacc aatatggccg ctatcagcgc
     4261 ggctgtcttg acgcgcccac cgagcagtca ctggtcgcgt atttacaaca aagagaattg
     4321 gagttgctga ccttcaagct tgggcggcct ttattaagat ggcagccacg cgtgaacata
     4381 tctgcgctga tcctgttttg tgtgcagatg gagcagttgt tacaagctgg ggtgcctttg
     4441 ctacaggcca tttccgaggt tgcagagcaa agcgaacaca aaggtctgca ggctgcattg
     4501 ctcactgtac agcgtgaact tgaggcaggt aaattgttat cgcaagcgtt gctggcgcaa
     4561 ccgaaaatat tcccgccttt atttggtcag ttgatcgatg caggcgagcg taccgggcag
     4621 ttgccggaga tttttgggca tttgaaccgc acattgacgt ggcaggcaga gctggtggcg
     4681 caaatccgcc gtggcctgac ttatcctgct gtgttgtgca tcatggtcgc ggtagccgcc
     4741 tatgtcatgc tgacttttct ggtgccacaa atggccagtt tcctgcaaag ccttggccaa
     4801 gatctgcctt ggtccacgcg tttgctacta ggactatcga atggattaat gaggtatggt
     4861 ggttggctac tgtttgccat cacagggctg acaggggctg ttgtattggc agtccgccat
     4921 tcaccccgat atgctttatt gctggatcag tgcttactta aattgcctgt tttgggcaga
     4981 ttgttgcatc aattggtatt ggcaaggctt tgccggtttc ttggcttgat gtaccagacg
     5041 ggtatccctt tattgcaggc gctggaatgg tgccagccac tattgcaaca gcgtttcatc
     5101 gccttggcgg tggcagaaat ccatcaacgt gtgctggccg gggagagtct cacgagcagc
     5161 ttcagagcca cgcagcaatt tccaccctta atgctgcgca tgctcagtgt cggtgaaaca
     5221 acaggcgcac tggatcgcat gttattgcaa ttggctgttt tttacgacag ggaggtgcag
     5281 tcacagctac aaaccttgtt acgcctctta gagccagcac tcactattgc gctcgggtgt
     5341 atgttgttat ttttaatggc agcagtgatg ctgcccgtgt acgacagctt ttccagtctg
     5401 cgctattaat catggctagg catcattttg ttgtcctgat tgaccaaagt gcggttatgc
     5461 gcgtcataca ttgtgttggg cagaatgtgt tggcgttgcg ggagtttgga ttcaccgatg
     5521 atagtgatgt tgcgttgcta cgctggtgtc aacagtttcc acaggcttcg gtttcatttc
     5581 tgagcaatct tgcggatgag cactatcacg tcgagacgct gccgcacgtg cgaggcgcag
     5641 cccaaaggca attattgcta cgtaagcttg cagcgtggcc tttcgcacaa gggatgtaca
     5701 cggcattccg tctgagtagt gtacaaaacc tgcgcaagga agatcgcttt ctgttcgttg
     5761 ctttattttg tccgccatta ttggcctggc tatccgggca acaaccccga tcgttgcgca
     5821 ttcagggaat atacacacag gcactcactt tgccttattg cttacctgcc ttgccaaaag
     5881 gtgtttcaca tcgtttatgt atccaatgca caccacacca tgtgcgaatc agctatctac
     5941 aacagcaccg attgtttttg agtcgtttca ttcctatgtc ccaagaggcg ctgcctgatc
     6001 ttgaggaacg ttttaatcgt attgcccaag aggcaaatca aatccgtatg acactgattc
     6061 agcagcgctg ggtagcggag gcagatatct tgcagattgt ctggctaggc caggaacccg
     6121 ctgatacgcg tggattaaaa aaacacttgc cggcacacgt tttgtgggta ttcatgccta
     6181 ccgttgagtt atcacgccag tcgggatgca gtccagtgct taaaggaata gatgtgatgg
     6241 attggatgtc gatacagcct atattgcgag gacgggcact ccctaacctg gctcctcaag
     6301 aggctttgct gcagagtagc atcattcgta ttaagcggta tgtgcaccag gcgggtgctg
     6361 caatggttgc gttgatgttg ctggctggtt ggatagctgt acaggcaacg caatctgatt
     6421 ttttgaaaat aaaacagctt aatcatcacc tgcaggcttt gcagcccgtg aagcctgtga
     6481 acggattgag tgaatcacag tggccacagg tgagtgtatt gacgcaaacg gtgcaaaagc
     6541 ttgaagcttc gttacgttca ccagcagatg ctttacaagc actgcaaaat ac
//
LOCUS       DATMSK010000154         5062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_58810,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000154 DATMSK010000000
VERSION     DATMSK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58810"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1733
                     /locus_tag="VNZ84_05180"
     CDS             3..1733
                     /locus_tag="VNZ84_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWU66381.1"
                     /translation="MLRMLVSDGHIDKAWAEKLHQDRKRDSSKIHPVVIVAEQKWQDL
                     QNPSQLLTVDSLSRFVAEKAKVPYYQIDPLKLDFSAAAQIVSQAYAERARIMPITVKD
                     GIATIATADPFHQEWLHDLERMLKLRIEVVMVNPLDLQRYLPEVYQLSAAIQSANAAK
                     AGQIVGVQNFEQLIELGKDRQLDANEQHVVNIVDWLFKYAFEQRASDIHLEPRRNMGL
                     MRFRIDGVLHQVYQLPSNIMNAITSRIKLLGRMDMVEKRRPQDGRVKTVGSDGKEIEL
                     RLSTMPTAFGEKLVMRIFKPDILVQDFQLLGFSKRQSEQWAQWTHQPNGIILVTGPTG
                     SGKTTTLYTTLKQLATSEVNVCTVEDPIEMIEPAFNQMQVTQNIGLGFAEGIRTLMRQ
                     DPDIIMVGEIRDQETADMAIQAALTGHLVLSTLHTNDAPSAVTRLLDLGVPAYLIHSS
                     LLGIMAQRLVRTLCPHCKTEAEISDEEWASVTRPFKAKKPAKTMKAVGCKECRQTGYR
                     GRTGIYEMLTLTPAIRKLITPETDLLQLRKVATQAGLQPLMLNGAEKVAAGLTTVEEV
                     LKVAPPIEMD"
     gene            complement(2021..3250)
                     /locus_tag="VNZ84_05185"
     CDS             complement(2021..3250)
                     /locus_tag="VNZ84_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU66382.1"
                     /translation="MFSAMLGEAWHAMGANRLRTFLTMLGMVIGVSAVVLMMAIGQGA
                     EASVKRSISAMGSHLFVVLSGPPRMGGARTATGNAPSLNVRDANAISELYGVVGVAPV
                     TMGKGQIVYASNNWNTDIIGTTSEYMDIRGWSLSAGYPFSSSDTRSATRVALLGKTVV
                     QNLFGEDGDIDPVGKTIRINQRPFVVLGVLDSKGQTLDGRDQDDTIIVPLTTAQRKLF
                     GNQLPGTVRQIMVQAESDNVMSLVEDALNSLLNQRHNIREGADSDFSVRNLTAIANSA
                     AETARTMSLLLGAIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARERDILMQF
                     LLEAIMISIVGCLIGIVIGVGGAILVSIFTQAEVIISTYSVLIAFSVAASIGVFFGFY
                     PARKAARLNPIEALRYQ"
     gene            complement(3243..3959)
                     /locus_tag="VNZ84_05190"
     CDS             complement(3243..3959)
                     /locus_tag="VNZ84_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU66383.1"
                     /translation="MQQPVISVNHLYKAYQTAAGDFPVLKDVNLQVLSGDYVAIMGPS
                     GSGKSTFMNILGCLDMPTAGSYVLDGYAVATMSADQVAKVRNQTIGFVFQGFNLLARA
                     SLLDNVSLPLVYAGVGKEERHARAEVLLEKVGLSKHVHSHPNQISGGQQQRVAIARAL
                     VNQPRLILADEPTGNLDSTTSEEIMAIFDGLNQEGITIVLVTHETDIAEHAKRQVRFL
                     DGKIVQDTSASASSGGEGHV"
     gene            complement(3964..>5062)
                     /locus_tag="VNZ84_05195"
     CDS             complement(3964..>5062)
                     /locus_tag="VNZ84_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU66384.1"
                     /translation="TVSANGTLNPVSLISVGTQVSGIVKKMYVDFNDVVKKDQVLMEL
                     DSRLFNAQIAQSQGNIRNNEAAVELAIANEKRMRQLYAQEYVSKQELDTAVQVLKSAK
                     AQLETARGTLARDVTNLNFSIIRSPVSGVVVNRVVDVGQTVAASFQTPTLIQIAQDLS
                     KMQIDTSFAEADIGKIKEGQKVNFNVDAFPDRSFDGVVKQLRLNSTNTSNVVTYNVVV
                     SVDNQDLTLLPGMTAYVNIIVDRARHALLVPNAALRYKPKLADAAKKPESPASDGEGG
                     KRSWRKHAAKGEGREPSPSNSVAGKIYVLRARQPVMVRVHTGITDGRSTVVTGKGLAA
                     GDKVIVGELQSDNTASKGQANNNGPRGPRMF"
BASE COUNT         1240 a         1459 c         1232 g         1131 t
ORIGIN      
        1 atttgctgcg catgctggtt tcagatggcc atatcgacaa agcctgggct gaaaaactgc
       61 atcaggaccg caagcgcgat agctccaaga tccatccagt ggtcattgta gccgagcaaa
      121 aatggcaaga cctgcaaaat ccgtcacagc tcctgacagt cgatagcctc agtcgttttg
      181 tcgccgagaa ggctaaagtt ccttattacc agattgatcc cttaaaacta gatttcagtg
      241 ccgcagcgca aattgtgtcg caagcctatg cagagcgtgc acgcatcatg ccgattacag
      301 tcaaggatgg cattgccacg attgccacgg cagacccttt ccaccaggaa tggctacacg
      361 acctggagcg catgctcaaa ttgcgcattg aagtggtcat ggttaatcca ttggatttac
      421 agcgttactt gcccgaggtt taccagcttt cggccgctat ccagtcagcc aacgccgcaa
      481 aagcgggcca gattgtcggc gtacagaact ttgaacaact gattgaactg ggcaaagacc
      541 gccagctgga tgcgaatgaa caacatgtgg tcaatattgt tgactggctg tttaaatatg
      601 cttttgagca acgcgccagc gacattcatc tggagccacg ccgcaatatg ggcttgatgc
      661 gcttccgtat tgatggggtg ttgcaccagg tgtaccagtt acccagcaat atcatgaatg
      721 cgattaccag ccgcatcaaa ctgcttggtc gcatggatat ggtggagaaa cgccgcccgc
      781 aagacggccg ggtcaaaacc gtgggtagtg atggcaagga aatcgaatta cgcttatcta
      841 ccatgcccac tgcgtttggc gaaaagctgg tcatgcggat tttcaaaccg gatattctgg
      901 tacaggactt ccagctattg ggcttctcca aacgccagag tgagcaatgg gcgcaatgga
      961 cgcatcaacc caatggcatt atcctggtca ccgggcccac cggctcaggc aagaccacca
     1021 cgctctacac gacactcaag cagctggcca ccagcgaagt taatgtatgt acggtagaag
     1081 acccgattga gatgatagag cctgcgttca accagatgca ggtgacacag aatattggat
     1141 tgggctttgc cgaaggcata cgcacgctga tgcggcagga cccggacatc attatggtcg
     1201 gcgaaatccg cgaccaggaa accgccgaca tggcgatcca ggcggccctg accggtcacc
     1261 tggtgctctc tacactgcac accaacgacg ccccctcagc cgtcacccgc ttactggacc
     1321 taggggttcc cgcttacctg attcactcca gcttgctcgg cattatggcg caacgcctgg
     1381 tacgcacttt gtgcccgcat tgcaaaaccg aagctgagat ttcagacgag gaatgggcca
     1441 gtgtgacacg cccgttcaag gccaaaaaac cagccaaaac catgaaagct gttggctgca
     1501 aggaatgccg ccagactggt taccgtggcc gcaccggtat ttatgaaatg ctgacgctca
     1561 caccggcaat ccgcaaactg attacaccgg aaaccgacct gcttcagtta cgcaaagtag
     1621 ccacccaggc ggggctgcag cctttaatgc tgaatggtgc ggagaaagtt gctgcgggac
     1681 tgaccacagt agaagaagtc ctgaaagtcg caccgcctat cgagatggac tagggcctgt
     1741 taacgctatt gcaattgtcg atgttacctc tgtcatcagt gctatcaagg cgtgagaagc
     1801 gcagtttggt tattcccaac aagcgccgaa caacgcggag agtgctgatg acagaggtaa
     1861 ccctgcgggc tggagtcatt tattcgcttc gctacgttgt ctatcgctta tgtagccagc
     1921 tagacccatc tatttcagcg actctttccg ccttgcgtaa cgcgtaaatg ctctccagca
     1981 gcggcaactg aaatagcgtt aacaggccct agctactcgt ttactggtac cgcaaggctt
     2041 ctatcggatt taggcgcgca gccttcctgg ccggataaaa gccgaaaaag acaccgatac
     2101 tcgccgccac gctaaacgca atcagcacag aataagtgga aataatcact tcggcctggg
     2161 tgaatatact aaccaatatt gcaccgccaa cgccgatcac aataccgatc aggcaaccca
     2221 caattgaaat catgatcgcc tcaagcagga actgcatgag gatatcgcgc tcccgggcgc
     2281 cgatggccat gcggatgccg atttctcgcg tacgctcagt cacagatacc agcatgatat
     2341 tcatgatgcc aataccccct accagcaaag acaccgaagc gatcgccccc aacagcaaag
     2401 acatggtgcg cgccgtttcg gccgcagaat tggcgatcgc cgtcaggttg cgtaccgaaa
     2461 aatcactgtc ggcgccctca cggatgttgt ggcgctggtt caacaagcta ttcagcgcgt
     2521 cttcgactaa gctcatcaca ttgtccgact cggcctgcac catgatctgc cgtactgtcc
     2581 ccggtagctg gttaccaaac agttttcgct gggcagtcgt cagcggcacg atgatggtgt
     2641 catcctgatc gcgcccatcc agcgtttgcc ctttactgtc cagcacgccc aatacgacaa
     2701 aaggccgctg gttaatacgt atggttttac caacaggatc aatgtcaccg tcttcgccaa
     2761 acaggttttg tacgacggtt ttacctaata atgccacgcg tgtagcagag cgtgtatcag
     2821 aagatgaaaa cggatatccg gcagacaaac tccagccacg gatgtccata tattccgacg
     2881 tggtgccaat aatatcggta ttccagttat tgctggcata gacaatctgg cccttgccca
     2941 tggtcaccgg ggccaccccc accacaccat atagctcgct aatggcattg gcatcccgca
     3001 cattaagtga cggtgcattc cccgtcgcgg tacgcgcacc gcccatgcgt ggtggcccgg
     3061 acaacaccac aaacaaatgg ctgcccatgg cactgataga gcgcttgacc gacgcctcag
     3121 cgccttgacc gatcgccatc attagtacaa cagcactcac accaatcacc atgcccaaca
     3181 tggtcagaaa cgtccgtaag cggttggcgc ccatggcatg ccaggcttcc cccagcatgg
     3241 cactaaacat gtccctctcc tccactggat gcactagcac tcgtgtcctg cacaattttt
     3301 ccatccagaa aacgcacctg ccgtttggcg tgctcagcaa tatccgtttc gtgcgtcacc
     3361 agtacaatgg tgatcccttc ctggttgagg ccatcaaaaa ttgccatgat ctcttcactg
     3421 gtggtgctat caaggttacc cgtcggctca tctgccagga taaggcgtgg ttgattgact
     3481 aatgcacgcg caatggccac ccgctgctgt tgtccgccag aaatctggtt aggatgcgaa
     3541 tgcacatgct tgcttaaacc taccttttcg agcagcactt cggcacgcgc atgccgctct
     3601 tccttaccta cgccggcata caccaatggc aggctaacgt tatcaagcag gctggcacgc
     3661 gccagcaggt taaagccttg gaacacaaaa ccgatggtct ggttacgcac cttggcaacc
     3721 tggtccgcac tcatggtggc gactgcataa ccatccagca cataactgcc ggcggttggc
     3781 atatcaaggc aacccaggat attcataaag gtagacttgc cggaaccgga aggccccatg
     3841 atggcgacat aatcaccgga cagcacctgc aaattgacgt cttttaatac gggaaagtca
     3901 cctgcagcag tctggtaggc tttatacaaa tgattcacag agatgacagg ctgctgcata
     3961 ccgttagaac attctcgggc cacgtgggcc gttattatta gcctgccctt tgctcgcggt
     4021 attatcgctt tgtaactcac cgacaatcac cttgtcaccc gctgccaggc ctttgccagt
     4081 cacgaccgtt gaacgtccat cggtaatgcc agtatgcaca cgcaccatga ctggttgcct
     4141 tgcccttaag acataaatct taccagccac gctgttggag ggagatggct cacgcccttc
     4201 acctttggca gcatgtttgc gccaagaacg cttaccaccc tccccatcgc tcgctggaga
     4261 ctctggtttc ttcgctgcat ctgctaactt cggtttgtag cgtagcgccg cattaggcac
     4321 cagcaaggca tgccgggccc ggtccacaat aatattcaca taggccgtca tgccaggcag
     4381 caacgtcaga tcctggttat ccaccgacac cacaacgtta taggtcacga cgttggaagt
     4441 gttggttgag ttcaggcgta actgtttcac cacgccgtca aaactacggt caggaaaggc
     4501 atccacatta aaattgacct tctgcccctc cttgattttg ccaatatctg cttcggcaaa
     4561 gctggtatca atttgcatct tggaaaggtc ttgcgcaatt tgaatcaggg tcggggtctg
     4621 gaaactggcc gccaccgtct ggccgacgtc gaccacccgg ttgaccacta ccccagacac
     4681 gggtgaacgg ataatactga aattcaagtt ggtgacatca cgtgccagcg tcccgcgcgc
     4741 ggtttccaat tgcgccttgg ccgactttaa tacctgtacg gcggtatcca gctcctgctt
     4801 ggaaacatac tcctgggcat ataactgccg catacgcttt tcattggcaa tcgccaattc
     4861 caccgctgct tcattattcc tgatgttgcc ctgagactgg gcaatctggg cattaaacaa
     4921 ccgactatca agctccatca gcacctggtc ctttttgacc acgtcattaa aatcaacgta
     4981 catctttttc acaatgccgg acacctgggt acccacactg atcaggctta cgggattgag
     5041 tgtgccattg gcagacactg tt
//
LOCUS       DATMSK010000155         8990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_222603,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000155 DATMSK010000000
VERSION     DATMSK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222603"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2201
                     /locus_tag="VNZ84_05200"
     CDS             <1..2201
                     /locus_tag="VNZ84_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU66385.1"
                     /translation="VNSMDGVLLDITESKHRNAEYEGIVNAMNYAVSAAEFTIDGVIL
                     NANDNFLTLLGYTLEEVTGRHHSILCPPDFISTDRYKQKWQALRRGEFVHGEFLRYGK
                     YDKQIWINAFYSPIRDIDGKVIKVLMFMMDISERKQMEQDLLRAKETAEQAANAKSTF
                     LANMSHEIRTPMNSIIGFSELLLDTPMQPEQRNYLSTVSQSAKSLLHLLNDILDSAKL
                     EKGMLELELLDFSIRELVDGLISSLWLQARKKNLDLRLNLDPQITGYYKGAEHRIRQV
                     LTNLLGNAIKFTETGYVELKVHLSPQGEVQFDIIDTGIGIDEDRLDVIFEPFTQADAS
                     MSRRFGGTGLGTTISKQLVELMGGRITASSKIGQGSCFSVSLPLAEGSAQEKALKNHL
                     LRSALPVLKILAADDIEQNRKLLSIVLEKQGHQVTLAQNGQEAIDAWQAQPYDVILMD
                     VQMPVMDGLNASMHIRAQEEKTGRTKTPIIALTASVLEQDRLAAAAAGMDGFASKPIE
                     LHLLLAEIQRVLQLEPLAITLPLKESAATTHHFDLHRGIALWGDQQTYLYEIHRYIQE
                     SRKHITMLTQAIAAQDHAALKQIAHANKGVSANLALSHIQESYHALEQSADGEWQTCT
                     QQVTQLQMHAQSLHAFITPLLSQQTVSVAIDEHALPITLLQDCLLALRTLVAMNELDD
                     ALLSKLTEHAPAEWRQALNKITQLLNEFDFEQATVHIDQLLEIQTQETSS"
     gene            2198..3196
                     /locus_tag="VNZ84_05205"
     CDS             2198..3196
                     /locus_tag="VNZ84_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HWU66386.1"
                     /translation="MMDHTMQQAKILIVDDEPLNLKVLKQVLQDNYRLSFAKNGIDAL
                     ELVHKEKPSLILLDVMMPGMTGFEVCRKLKNDPETNTIPVIFVTALAEEADESEGFAA
                     GGVDYINKPISPSLVRARIKTHLSLVHIDELKRTHLELIQRLGRAAEYKDNETGMHVI
                     RMSHTSGRIALQMGFDAHNAEQLVHAAPMHDIGKIGIPDHVLLKPGKLDEEELKTMRT
                     HPQIGAEILDNSSSPLIRLAHKVALYHHEKWDGSGYPFGLKGEDIPIEARIVAVADVF
                     DALLSKRPYKEAWPVEKAVEEIKSQTNRHFDPGVVEAMMACLPDLIAINEKWREED"
     gene            complement(3257..4702)
                     /gene="gatB"
                     /locus_tag="VNZ84_05210"
     CDS             complement(3257..4702)
                     /gene="gatB"
                     /locus_tag="VNZ84_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWU66387.1"
                     /translation="MQWEVVIGLETHTQLQTKSKQFSGASTTYGAEPNTQACEVSIAL
                     PGVLPVLNKEAVECAIKFGLAIDAEMAPRSVFSRKNYFYPDLPKGYQISQFELPVVGK
                     GQITIQVPATKNTEAYEKVINITRAHLEEDAGKSVHGAVEGMSGIDLNRAGTPLLEIV
                     TEPDMRSAAEAVAYAKKLHELVQWIGICDGNMQEGSFRCDVNVSVRPKGSEKFGTRRE
                     IKNLNSFKFMQQAIEYETQWQIETLEDGGTIQQATVLFNPDTGETRAMRTKEEANDYR
                     YFPDPDLLPLEVTEADKARVRAAMPELPQALQARFISDYQLSSYDASTLTASRSLADY
                     FVAVVNAGAEPKQAANWIMGALSAKLNAEEKTITQSPVTATQLAALLKRIADNTISNN
                     AAKQVFEGLWAGEGEVDAIIEAKGLKQVSDTGAIEAIINEVLAANQAMVDEYKAGKEK
                     AFNALVGQCMKASKGKANPAQVNELLKKALG"
     gene            complement(4703..6184)
                     /gene="gatA"
                     /locus_tag="VNZ84_05215"
     CDS             complement(4703..6184)
                     /gene="gatA"
                     /locus_tag="VNZ84_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HWU66388.1"
                     /translation="MINSSLKQLGQQLASKQVSSVELTQAYLQRMAQLNPSINAYVTI
                     DEAKTLAQAKAADARIAAGDFGPLTGIPLAQKDIFCAKGWRTTCSSKMLETFISPYDA
                     HVISQFDAAGAVNLGKTNMDEFAMGSSNETSYFGGVKNPWDVDRVPGGSSGGSAAAVA
                     ARLCAAATGTDTGGSIRQPASLCGFTGLKPTYGLVSRYGMIAFASSLDQAGPMAKSAE
                     DCALMMNVMAGFDERDSTSVNREKEDYTRGLDKMTGEQPLKGLRIGLPKEYFADGLNA
                     DVAKVIETAIAEYKKLGAEIVEVSLPNNNLSIPVYYVLAPAEASSNLSRYDGVRYGHR
                     AAEYNDLMEMYMKSRAEGFGAEVKRRILIGTYVLSAGYYDAYYLKAQKIRRLIAQDFV
                     EAFKHCDVIMGPAAPSPAFKAGEKTSDPVAMYLEDLYTIAVNLAGLPGMSIPAGFATS
                     SEGKALPVGLQIIGNYFDEARMLDIGHAYQQVTDWHTRMPEGI"
     gene            complement(6236..6523)
                     /gene="gatC"
                     /locus_tag="VNZ84_05220"
     CDS             complement(6236..6523)
                     /gene="gatC"
                     /locus_tag="VNZ84_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWU66389.1"
                     /translation="MALNLDDIKRIAHLARIEVSDNEAQETLNKLSGILGLIEQMQAV
                     DTTGIEPMSHSQDVTQRLREDVVTATNQRELFQSIAPATQDGLYLVPKVIE"
     gene            6652..7695
                     /locus_tag="VNZ84_05225"
     CDS             6652..7695
                     /locus_tag="VNZ84_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWU66390.1"
                     /translation="MFGSLNSYFSNDLAIDLGTANTLIYVRGKGVVLDEPSVVAIRTE
                     GGPNGKKVLLAVGSDAKSMLGRAPANIQAIRPMKDGVIADFTITEQMLKFFIRKVHES
                     RWFSPSPRIIICVPVGSTQVERRAIKESAERAGAKQVFLIEEPMAAAIGADMPVAEAT
                     GSMVVDIGGGTTEVGVISLGGIVYAKSERVGGDKFDQAIIDYIRRNYGMQISEPTAEA
                     IKKRIGSAFPGSEVLEMEVTGRNLAEGLPRKFTISSNEILEALTEPLNSIVGAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDLDRLLVEETGLPVIVAEDPLTCVARGCGRAL
                     EEMDKLGNVFASE"
     gene            7725..8813
                     /gene="mreC"
                     /locus_tag="VNZ84_05230"
     CDS             7725..8813
                     /gene="mreC"
                     /locus_tag="VNZ84_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWU66391.1"
                     /translation="MLKGIHQQLDPQESPAFFIQGPKPFSRLMFFCALSLVMMAVDAR
                     FDYLGQVRQWFSTALHPLEVVASAPNEWARNINTYFTSHNQLIQDNFKLKQQALIDHA
                     ALQRLATVDAENAHLRTLLAGDAPIIPQATLGEVMHMGRDPFSNIITLNRGSHHQITP
                     GQAVVDEQGVVGQITRVYPFTSEVTLITDKKLSIPIQIERNQLRAIAFGEGKRNTLDI
                     PYLPTSVDIKVGDKLVTSGIDGVYPAGLAVAVVTQIKQSQDSPFAKIISRPIAGVNNH
                     KHVLILNIPDSPATSPSSENRVVLPRSNAQHLDQSVVDVLQKHNAAVDPHPATETPVL
                     DAHNPEANPASPETTPGNTTAPASPERP"
     gene            8810..>8990
                     /gene="mreD"
                     /locus_tag="VNZ84_05235"
     CDS             8810..>8990
                     /gene="mreD"
                     /locus_tag="VNZ84_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWU66392.1"
                     /translation="MKPVKFSTFLFTMLIGLIFQLYPWSNSGIIIRPDFLLVIMLYWL
                     LQAPYHCNIGWVWFAG"
BASE COUNT         2229 a         2405 c         2216 g         2140 t
ORIGIN      
        1 aagtaaactc catggatggc gtgttgcttg acatcacgga atccaagcac aggaacgcgg
       61 agtatgaagg catagtcaac gccatgaact acgccgtcag cgccgcagaa ttcaccattg
      121 atggcgtgat cttgaatgcc aatgataact tcctgacatt actgggctat acactggagg
      181 aggtgactgg ccgtcaccat tccatactct gcccacctga ctttatttcc acagaccgtt
      241 ataaacaaaa atggcaggca ttacgacggg gcgagttcgt gcatggcgaa tttctgcgtt
      301 atggcaaata tgacaaacag atctggatca atgcctttta cagcccgatc cgcgacatcg
      361 acggtaaagt gatcaaggtc ctgatgttta tgatggatat cagcgagcgc aaacaaatgg
      421 aacaagacct gttgcgcgct aaggagacgg ctgagcaggc cgccaatgct aaaagcacct
      481 tcctggccaa tatgagccat gaaattcgca cacccatgaa ttcgattatc ggttttagcg
      541 agctcttgct cgatacgcca atgcaaccag aacagcgtaa ttatttgtct actgtcagcc
      601 agtcagccaa atccttactg cacttgctta atgacatcct ggatagcgcc aaacttgaga
      661 aaggcatgct agagctggaa ttgctggact tttccatacg cgagctggtc gatggcctga
      721 tttcttcgct ttggctccag gcacgcaaaa agaatctgga tttgcgcctg aacctggacc
      781 cgcaaatcac tggttattac aaaggcgccg agcaccgtat acgccaggtt ttgaccaacc
      841 tgctgggcaa cgccatcaaa tttaccgaaa ccggctacgt tgaactcaaa gtacatctct
      901 ctccccaagg tgaagtgcag tttgacatta ttgatactgg catcggtatt gatgaagacc
      961 ggctggatgt gatttttgaa ccatttacac aggccgatgc ttcaatgagt cgtcgctttg
     1021 gcggcacggg tctggggacg acgatcagca aacagctggt agagctgatg ggggggcgca
     1081 tcaccgccag cagtaaaatt ggtcaaggca gttgcttctc ggtgagtttg ccactggctg
     1141 aagggagtgc gcaggaaaaa gcactgaaaa accacctact cagatcggca ctgcctgtgc
     1201 tgaaaatact tgctgcggat gatattgaac aaaaccgcaa gctgctgagc attgtgctgg
     1261 aaaagcaagg gcatcaagtg accttggccc agaatggcca agaggccatt gatgcctggc
     1321 aagcccagcc atacgatgtg atcctgatgg atgtgcaaat gcctgtcatg gatggcctaa
     1381 acgccagtat gcatattcga gcgcaggaag agaaaactgg ccgcaccaaa acgccaataa
     1441 ttgcattgac cgccagcgtg cttgaacaag accggctggc agccgccgcg gcaggtatgg
     1501 atggctttgc ctccaaaccc attgagctgc acttgttact ggctgaaatc cagcgcgtgt
     1561 tgcagctgga gcctctggct atcacacttc cactcaaaga atctgcagca acaacgcatc
     1621 attttgacct gcatagaggc atagccttgt ggggtgacca gcaaacctac ctgtatgaaa
     1681 tccatcgtta catacaagag tcacgtaaac acatcacgat gctaacacaa gcgatcgccg
     1741 ctcaagatca cgccgcattg aaacagattg cccatgccaa caaaggcgtt agcgccaatc
     1801 ttgcactatc tcatattcag gaaagttacc atgctctgga acagagcgcc gatggcgaat
     1861 ggcaaacctg cactcagcaa gtgacgcaat tacaaatgca cgctcagtca ctacatgcgt
     1921 tcatcacccc tttgctatcg cagcagacgg taagtgtggc cattgacgag cacgctcttc
     1981 caattaccct gctgcaagac tgtctgctcg cgctacgtac actggttgcc atgaacgaac
     2041 tggacgatgc attgctcagc aaactcaccg agcatgctcc ggccgaatgg cgccaggcat
     2101 tgaacaagat tacccagcta ctcaatgaat ttgactttga acaagcgact gtgcatattg
     2161 accagctatt ggaaatccag acgcaggaga cctcctcatg atggatcaca cgatgcaaca
     2221 agcaaaaatt ctgattgttg acgatgagcc actcaacttg aaagtactga agcaggtgct
     2281 gcaagacaat taccgtctga gctttgccaa aaatggcata gatgcattgg aactggtcca
     2341 caaagagaaa cccagcctga tattactgga tgtcatgatg cctggcatga cggggtttga
     2401 ggtttgccgt aaattaaaaa atgatccaga aactaatacc atcccggtga tttttgtcac
     2461 ggcactggca gaagaagccg atgagtccga aggttttgcg gcgggcggcg ttgattacat
     2521 caacaaaccg atttcaccct cgctggtccg cgcacgcatc aaaacacatt tatccctggt
     2581 acatattgat gaactcaagc gtacacacct ggaactgata caacgcctgg gacgtgcggc
     2641 agaatataaa gacaatgaaa ctggtatgca tgtaatccgt atgagccata cctctggcag
     2701 aatcgcgctt caaatggggt ttgatgcaca caatgccgaa cagttagtgc atgccgcacc
     2761 catgcatgat atcggcaaga tcgggattcc tgaccatgtg ctgctgaaac cgggcaaact
     2821 cgatgaagaa gaacttaaaa ccatgcggac acatccacaa attggtgcgg agattctgga
     2881 caactcatcc tcgcccttga ttaggctggc ccataaggtg gcactttacc accatgaaaa
     2941 atgggatggc agcggttatc cttttgggtt aaaaggcgaa gacatcccaa ttgaagcgcg
     3001 tattgtcgcg gttgcggatg tgtttgatgc tctcttgagt aaacggcctt acaaagaggc
     3061 atggcccgtt gaaaaggccg tagaagaaat caagtcacaa acaaaccggc attttgatcc
     3121 cggcgtggta gaagcaatga tggcctgttt gccagatttg atcgccatta atgaaaaatg
     3181 gcgcgaagaa gactgatgtg cttcctcacg ccatctacac tcctctcgct aatttccatt
     3241 agcgagcagg cgagcattac cccagtgcct ttttcaacag ttcgttgact tgtgccggat
     3301 tggccttgcc ttttgaagct ttcatgcact ggcccaccag cgcattaaaa gctttttcct
     3361 tacccgcttt atactcatcc accatggcct ggttagcggc cagcacttca ttgataatcg
     3421 cttcaatggc gcccgtatcc gacacttgct tgaggccttt ggcttcaatg atcgcatcca
     3481 cttcaccctc acctgcccac aagccttcaa acacctgctt ggccgcgttg ttggaaatgg
     3541 tgttatccgc aattctttta agtaaagcgg ccagttgggt ggcagtcacc gggctctgag
     3601 taattgtttt ttcttccgca ttcagtttgg cggataaggc acccatgatc cagttcgctg
     3661 cctgcttagg ctctgcgcca gcattaacca cggccacgaa ataatctgcc aagctgcgtg
     3721 atgccgtcag cgtgctggca tcgtaacttg agagctggta atcactgata aaacgcgcct
     3781 gcagcgcttg cggcagctca ggcattgctg ccctgacgcg cgctttgtcc gcttcagtga
     3841 cttctagtgg cagcaagtct gggtcgggga agtaacggta atcgttagcc tcttccttgg
     3901 tacgcatggc gcgggtttca ccggtatctg gattgaacaa gacagtcgct tgctgaatgg
     3961 taccgccgtc ttccaatgtc tcgatttgcc attgggtctc gtactcaatc gcctgctgca
     4021 tgaacttgaa ggagttcagg tttttgattt cgcggcgtgt accaaacttc tcgctgcctt
     4081 ttgggcgcac agacacgttt acatcacaac ggaaactacc ttcctgcata ttaccgtcac
     4141 aaatacctat ccattgcacc agttcatgca gttttttggc gtaggccacg gcttcagccg
     4201 cgctacgcat atccggctcg gtcacaattt ccagcaacgg cgtccccgca cggttcaagt
     4261 cgatgcctga catgccttcc acagcgccat gtacagattt accggcatct tcttccagat
     4321 gcgcacgtgt gatattgatg actttttcgt aggcttcggt atttttagtc gcgggcacct
     4381 gaatggtaat ttgcccttta cccaccaccg gcaactcaaa ctggctaatc tggtagcctt
     4441 taggtaagtc tggatagaaa taatttttgc gcgaaaacac actgcgtggt gccatctcgg
     4501 catcaatcgc taggccaaat ttaatcgcac attcgacggc ttctttattc agcactggca
     4561 aaacaccggg taaagcgatg ctcacttcgc aagcctgggt atttggctca gcgccataag
     4621 tcgttgaagc gccgctaaat tgcttggatt tagtttgtaa ctgagtatgg gtttccaacc
     4681 cgatcacgac ttcccactgc atttaaattc cctccggcat acgtgtgtgc cagtctgtca
     4741 cttgctgata ggcgtggcca atgtccagca ttcgcgcctc atcaaaataa ttaccaataa
     4801 tctgcaaacc gaccggcaag gctttacctt cgctactggt ggcaaaaccg gcaggaatac
     4861 tcatgcctgg caaaccggcc aaattgacgg cgatggtgta caaatcttcc aggtacatgg
     4921 cgaccggatc actagtcttc tccccagctt taaaggctgg cgaaggcgca gcaggcccca
     4981 taatgacatc acaatgcttg aacgcttcga caaagtcctg cgcaatcagg cgacggattt
     5041 tctgcgcctt gaggtaataa gcatcgtagt aacccgcgct cagcacatag gtaccaatca
     5101 ggatacgtcg cttcacctcg gcaccaaagc cttcggcgcg tgacttcatg tacatttcca
     5161 tgaggtcgtt atattcagct gcacggtgac cgtaacgcac accatcatag cgtgacagat
     5221 tgctggaagc ctctgccggc gccagcacgt aatacactgg aatcgacaga ttgttattag
     5281 gcaaactgac ttcaacaatt tctgcaccca gttttttgta ttcggcaata gcggtttcaa
     5341 tgacttttgc cacgtcagca ttcaggccat cggcaaagta ctctttaggc aggccgatac
     5401 gtaaaccttt gagcggctgt tcgcctgtca tcttgtccag gccgcgggtg taatcttctt
     5461 tttcacggtt gacgctggtg gaatcacgct catcaaagcc ggccatgacg ttcatcatca
     5521 aggcacagtc ctctgccgat ttagccattg ggccggcctg gtccagactg gaagcaaaag
     5581 caatcatgcc gtaacgcgac accaggccat acgtcggctt caaaccggta aagccacaca
     5641 aggaagccgg ctgacgaatt gaaccaccgg tatcggtccc cgtcgctgcc gcacataagc
     5701 gcgcagcgac tgcggctgcc gaaccaccgg aactaccgcc tggtacacgg tcaacatccc
     5761 atgggttttt cacgccaccg aagtaactgg tttcgttaga cgagcccatg gcaaattcgt
     5821 ccatgttggt cttgcccaga ttgaccgcac cggcagcatc aaactggctg atcacatgcg
     5881 catcgtacgg agagataaac gtctccagca ttttcgagct acaggttgtg cgccagcctt
     5941 tggcacagaa aatatccttc tgtgccagcg gaatcccggt caacggtccg aaatcaccgg
     6001 ctgcgatacg cgcatcagcg gctttggcct gtgccaatgt tttggcttcg tctatggtga
     6061 cgtaggcatt gatgctagga ttgagctgcg ccatgcgctg caaataggct tgcgtcagct
     6121 cgacgctgga aacttgcttg ctggccaact gttggcctag ctgtttaaga ctgctgttga
     6181 tcatggctag ggtatctgta aattaatgac gtcaactcgc gtagaagcga cgctgttatt
     6241 caatgacttt gggcaccagg tacaaaccat cctgcgttgc cggagcaatg gattgaaata
     6301 gctcacgctg gttggtagcg gtgaccacgt cttcacgcag acgctgtgtc acatcttgtg
     6361 agtgcgacat cggttcaata ccggtagtat caactgcctg catttgttcg atcaaaccga
     6421 ggataccgga gagtttgttc agggtttctt gcgcttcatt gtcactgacc tcgatacggg
     6481 cgaggtgagc gatgcgcttg atatcatcta gattcagtgc catgtctggt taattgtctg
     6541 taagattttg aaagcacatc ttaatttcat gtgcgttttg cgctattata ccttgatttt
     6601 atgccgcact tagcgcgcag ttctaaagtc ggaaacttca caggataaaa catgttcggt
     6661 tcactcaaca gctatttctc caatgacctt gccattgacc taggcaccgc caacaccctg
     6721 atttacgtgc gtggtaaagg cgtcgtactg gatgagccgt cggtggtggc gattcgtacc
     6781 gaaggtggtc ctaacggcaa aaaagtattg ctggctgtcg gttctgacgc gaaaagcatg
     6841 ctgggccgtg cgccagccaa catccaggcg attcgcccga tgaaagatgg tgtgattgcc
     6901 gacttcacca tcaccgagca aatgctcaag ttttttatcc gtaaagtaca cgaatcccgc
     6961 tggttctccc ctagcccacg catcatcatt tgtgtgcctg taggatccac acaagtggag
     7021 cgccgcgcga ttaaagagtc tgccgagcgc gcaggtgcca aacaggtatt cctgattgaa
     7081 gaaccaatgg cggcggcgat tggcgccgac atgccagtgg ctgaagcgac cggctccatg
     7141 gtggtggata ttggcggcgg cacgacagaa gtcggtgtga tttcactggg cggcattgta
     7201 tatgccaaat ccgagcgtgt cggtggcgac aagtttgacc aggcgattat tgactatatc
     7261 cgccgtaact acggcatgca gatttcggaa cccactgctg aagcgatcaa gaaacgtatc
     7321 ggttctgcct tcccgggctc tgaagtcctc gaaatggaag tgaccggccg caacctggca
     7381 gaaggcttgc cacgaaaatt cacgatttcc agcaacgaga tcctggaagc cctgactgaa
     7441 ccactgaatt ccatcgttgg tgcggtgaaa tccgcccttg aacaaacgcc accagaactg
     7501 ggtgccgaca ttgctgaaaa agggatggtt ctcactggtg gtggcgcctt gctgcgcgac
     7561 ctggaccgct tgctggtgga agaaaccggc ttgcctgtca ttgtggctga agacccttta
     7621 acctgtgtgg cacgcggctg cggccgagcc ctcgaagaaa tggataagct gggcaacgtt
     7681 tttgctagcg aataatccgg ggcaaccttg gcgcatcccc gatcatgcta aaaggcatac
     7741 accagcagct tgacccacag gagtccccgg ccttttttat ccagggaccc aagcctttct
     7801 cacgcctgat gtttttttgc gccctgtcat tggtcatgat ggcggtggat gcgcgttttg
     7861 actacctggg ccaggtcagg caatggttca gtaccgccct acatcctctc gaagtggttg
     7921 ccagtgcgcc aaatgaatgg gcacgtaata tcaacaccta ttttacttcg cacaaccagc
     7981 ttatccagga taactttaag ctcaagcaac aggccttgat tgatcatgca gccttgcagc
     8041 gcctggcaac ggtagatgca gaaaatgccc atttacgcac cctgctagct ggcgatgcac
     8101 cgattatccc gcaggcaacg ctaggcgaag tcatgcacat gggccgtgac cctttcagca
     8161 acatcatcac ccttaaccgc ggcagccatc accagattac gcccggccag gccgtggttg
     8221 atgaacaggg cgtggtcggg caaatcacgc gtgtttatcc attcaccagc gaagtgacgc
     8281 tcattaccga caaaaaactg tccatcccga tccagattga acgcaaccag ttacgtgcga
     8341 ttgcctttgg tgaaggcaag cgcaacacgc tggatatccc ttacctgcca accagtgtag
     8401 atattaaagt cggtgacaaa ctggtcactt cgggcattga tggcgtgtat cctgccgggt
     8461 tggctgtggc agtcgtcacc caaatcaaac aaagccagga ctcgccattt gccaaaatca
     8521 tcagccgccc gattgccggc gtcaataatc acaaacatgt gttaatcctc aatattccag
     8581 attcaccggc aaccagccca tccagcgaaa acagggttgt ccttccgcgt agcaatgccc
     8641 agcacctgga ccaatccgtg gtcgatgtac tgcaaaaaca taatgcggcg gtagacccgc
     8701 accctgccac cgaaacaccc gtgcttgatg cacacaatcc tgaagcaaat cctgccagcc
     8761 cagagacaac gcccggcaac accacggcac ccgcctcacc ggagcgacca tgaagccggt
     8821 caaatttagt acattcctgt ttaccatgct gatagggctg atttttcagc tatacccctg
     8881 gtctaattcc ggcattatta tccggccaga ctttttgcta gtcattatgc tttactggct
     8941 attgcaggct ccttaccact gtaatattgg ctgggtgtgg tttgccggct
//
LOCUS       DATMSK010000156         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_81401,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000156 DATMSK010000000
VERSION     DATMSK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81401"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1954)
                     /locus_tag="VNZ84_05240"
     CDS             complement(<1..1954)
                     /locus_tag="VNZ84_05240"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HWU66393.1"
                     /translation="MLKAVEQKKVHSEEVAAEIPVQPVSLDIWDKKYRLKTKSGEHVD
                     KDMDDSYSRVARALADVEITEEKKHEWHEKFLWALRRGAIPAGRITSNAGAQEHKPAT
                     STINCTVSGVVEDSMDDILGKVHEAGLTLKAGCGIGYEFSTLRPKGAFVAGAGAYTSG
                     PLSFMDIYDKMCFTVSSAGGRRGAQMATFDISHPDVTDFIKAKRENGRLRQFNLSCLI
                     TKEFMEAVKADAEWRLAFPVTEKEAIIDGLNVNDESQVVWRDWPIKGKYLTKTEGPEA
                     GKVACRIYKTIKARRLWDVIMSSTYDFAEPGFILIDRVNEMNNNWFCENIRATNPCGE
                     QPLPPYGACLLGSVNLTKFVRQPFTNDAHFDWDEYREVVGVFTRMLDNVVEINGLPLE
                     QQRQEIARKRRHGMGYLGLGSTLTMLKMKYGEEDSVKFTEEVTRIMAEVGWEVGVQLA
                     EEKGPAPIMDEKFEVTADMLRVRPEMAADGIKVGQKLAGKVLLGKYSRYMQQFPEGLR
                     NQIATKGVRFTHHSSIAPTGTISLSLANNASNGIEPSFAHHYARNVIREGKKSKEKVD
                     VFSYELLAYRELVNPNAMPFSDKPEEQLPDYFLDSSTIMAKAHVDIQAAAQKWIDSSI
                     SKTINVPTDYDFEDFKKIYLYAYEKGL"
     gene            2331..3596
                     /gene="waaA"
                     /locus_tag="VNZ84_05245"
     CDS             2331..3596
                     /gene="waaA"
                     /locus_tag="VNZ84_05245"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HWU66394.1"
                     /translation="MPKFVYTLLMTLVALLLPLKLFWRGLKQPEYRQHVGERYGFYSQ
                     QVHAPVIWVHCVSVGETRATQPLIQALLAAYPQHQVLITHGTPTGRDTSSQLFKQEIA
                     HGRVLQAYLPVDTPGASQRFLAHFKPVLGLLMETELWFHLINQSIKSNIPMALLSARL
                     SAKSAAGYAKLGSLTRQGLQQLALVAAQTAADAERLQVLGANHPMICGNLKFDVTPPE
                     DSTAKGKALRQLLGENRTVCMAASTREGEESLILDAVQGTCVLTLLVPRHPQRFAEVE
                     SLLKSRGLHYVLRTELTQPVSDDVQVVLGNSMGEMYTYYAASDFAVMGGSLQPLGGQN
                     LIEAAAMGVPTILGPNMFNFTQVAQAAVLADAAVQLKEVSELHGMIATLAADKVKVAT
                     MSLAAKGFTLQHRGTTERLMAAIAPLLKR"
BASE COUNT          840 a          992 c         1012 g          949 t
ORIGIN      
        1 tgaggccttt ttcatacgca tagaggtaga tttttttaaa atcctcaaag tcgtaatcgg
       61 tgggaacgtt aatggtttta gagatggagc tatcaatcca tttttgcgcg gcagcctgaa
      121 tatccacgtg ggctttggcc atgatggtgg atgaatcgag gaagtaatct ggcaactgct
      181 cttctggctt gtcgctaaac ggcatggcgt ttgggttcac cagttcacgg tatgccagca
      241 actcgtagct gaatacatcg actttctctt tagacttttt gccttcacga atgacgttac
      301 gcgcatagtg gtgggcaaag ctcggctcga tgccgttgct ggcgttgttc gccaagctca
      361 gtgaaatcgt gccggttggc gcgatggagc tatggtgcgt gaaacgtaca cctttggtcg
      421 cgatctggtt acgcaggcct tccgggaact gctgcatgta gcggctgtat ttacccagca
      481 atactttacc cgccagtttc tggccgacct tgatgccatc cgcagccatt tctgggcgta
      541 cgcgcagcat atcggcagtg acttcaaact tctcatccat gatcggtgcc gggccttttt
      601 cttcagccag ttgcacgccg acttcccagc ccacttcggc catgatgcgg gtgacttctt
      661 cagtgaattt cacggaatct tcttcaccgt atttcatttt cagcatggtg agtgttgagc
      721 ccaggcccag gtaacccata ccgtgacggc gtttacgggc gatttcctgg cgctgctgct
      781 ctaatggcaa gccgttgatc tccaccacgt tatccagcat gcgggtgaac acgccaacga
      841 cttcgcggta ttcatcccag tcaaaatgtg cgtcattagt gaatggctgg cgcacgaact
      901 tggtcaggtt gacggagccc agcaggcagg cgccgtaagg tggcaatggc tgctcgccac
      961 aagggttggt cgcgcggatg ttttcgcaga accagttatt gttcatctcg ttgacgcggt
     1021 cgatcaggat aaaccctggc tcagcaaagt cgtaggtgga agacatgatg acatcccaca
     1081 gacggcgcgc cttgatggtc ttgtaaatgc ggcaagccac tttaccggct tctgggcctt
     1141 cggttttggt gaggtatttg cctttgatgg gccagtcacg ccacaccact tgtgattcgt
     1201 cattcacatt caggccatca ataatggctt ctttttcggt gacagggaaa gccaggcgcc
     1261 attcggcatc ggctttcacc gcttccataa attctttggt gatcaggcag ctcaggttga
     1321 actggcgcaa acggccgttt tcacgcttgg ccttgataaa gtcagtgacg tctggatgcg
     1381 aaatatcaaa ggtcgccatt tgtgcgccac gacgaccacc ggctgaagac acggtgaaac
     1441 acattttgtc gtagatatcc ataaaagaca gcgggccgga agtataggca ccggcacctg
     1501 cgacaaacgc gccttttgga cgcagcgtgg agaactcgta accgatacca caaccggctt
     1561 tgagggtcag gcccgcttcg tgcactttgc ccagaatatc gtccatgctg tcttcgacta
     1621 cgccagatac ggtgcagtta atggtagaag tggcaggttt gtgctcttgc gcaccggcgt
     1681 tggaggtaat gcggcctgcc ggaatcgcgc cacggcgcaa cgcccacagg aatttttcgt
     1741 gccattcgtg ctttttctct tcggtgattt caacatcggc cagcgcacgt gccacgcggc
     1801 tgtaagaatc gtccatgtct ttatccacat gctcaccgga tttggttttg agacggtatt
     1861 ttttatccca gatatcaagt gagacaggct gcaccggaat ctcggctgcg acctcttctg
     1921 aatggacttt cttttgttca actgctttca gcattgagtt ctcccacctt tggtgtttct
     1981 tattcattga tagcgcattg caaaccagaa taacccctgt ggtttacaac gcgttggcac
     2041 gtagatcggc ttaaaacctg gcgttgcgcc gctaattttg ctgcacgcga cagattgcgt
     2101 tgcaccaccg atcaaacaca acatcttgtg ttggtggcga taatttatgc ctatattttg
     2161 ttgacgtcaa tgcttttttt gcgccttttt ttggtgcgta tggcttgcat tttcgcaatg
     2221 acttttagta caagggctca ggcataggtg agtcatcact ctcgcgtttt gcatgcattt
     2281 gattggtgca cttcagcgtg gtatttgcac aaatatgcga gaatagcgcc atgccaaaat
     2341 tcgtgtatac cttactgatg acactcgtgg ccttgctgct gccgctcaag ctgttctggc
     2401 gcggcctcaa gcagccagag taccgtcagc acgtgggtga gcgctatggc ttttacagcc
     2461 aacaagtaca tgcgccagtg atctgggtgc attgtgtttc agtcggtgaa acacgcgcca
     2521 cgcagccgct gatccaggca ttgctggctg catatccgca acaccaggta ctgatcacgc
     2581 acggcacacc gaccggccgc gacaccagca gccagctatt caagcaggaa atcgcccatg
     2641 gccgtgtgct gcaagcttat ttgccggtgg atacgcctgg cgccagtcaa cgcttcctgg
     2701 cgcacttcaa gcctgtatta ggtttgttaa tggaaaccga gttatggttc cacctgatca
     2761 atcaatccat aaaatcaaac atccccatgg ccttgctcag cgcccgcctc tcggccaaat
     2821 ctgctgcggg ttacgccaag cttggtagcc tgacgcggca aggtctgcaa caactggcgc
     2881 tggtcgccgc gcaaactgcg gccgatgccg agcgccttca agtactgggg gcaaaccacc
     2941 ccatgatctg cggtaacctt aaatttgatg tcactccgcc agaggacagt actgccaaag
     3001 ggaaagccct gcgccaacta ctgggtgaga accgcacagt atgtatggcc gccagcacac
     3061 gcgaaggcga agagtcattg atcctggatg cggtacaagg cacctgtgta ctcacactat
     3121 tggtgccccg ccatccgcaa cgctttgccg aagtggaatc gttactgaaa tcacgcggcc
     3181 tgcactatgt gctgcgcaca gaactcacgc agcctgtaag cgacgatgtg caagtcgtgc
     3241 tcggcaacag catgggcgag atgtatacct attatgcggc gagtgatttc gcggtaatgg
     3301 gtggtagcct gcaaccgctg ggcgggcaaa acctgattga agcggcggcc atgggtgtac
     3361 cgacgatatt agggccaaat atgtttaatt ttacgcaggt ggcgcaagcg gccgtgcttg
     3421 cggatgcggc tgtgcaatta aaagaggtga gtgagctgca tggcatgatt gcaaccttgg
     3481 cagcggataa ggtaaaagta gcgacgatgt cactggcagc gaaggggttt acgttgcagc
     3541 atagagggac aacggagaga ttgatggcgg cgatagcgcc tttattaaaa cggtaattct
     3601 ccacgttgaa agtggcttgc gaatatagaa atatcgccga acaaggtcac atctaatttc
     3661 gcctcttggc gagttacttt ctgttgcttg ccccacagaa agtaaccaaa gaagacggca
     3721 cccagccatc acggccttgc ggctcccttg cgttgctcaa caaagtaagc ggtcacgaaa
     3781 ctcgccctaa cag
//
LOCUS       DATMSK010000157        12706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_387931,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000157 DATMSK010000000
VERSION     DATMSK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 12706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12706
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387931"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1108)
                     /locus_tag="VNZ84_05250"
     CDS             complement(221..1108)
                     /locus_tag="VNZ84_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWU66395.1"
                     /translation="MKSVTFPLLAIMMWSGNVLVSKLAAGLIHPIAMTFCRLLVALIV
                     MALFLLPSIWRNRAVIIQSLPKLAVLGFLSMAFYQCLSYQAALTTTANHMAVMTALNP
                     LLTMLVSAGLLGEAISVGMISGGLTALLGITILISHGQPMSLVQEGAPQGDVLMLMAS
                     LVYALYSVLIRKWAFPLSAWQSTFMQALCVCLCMGVALLFTRPAINVNAHTLPLILYA
                     GVLASVLLPYFWIKGVLLIGPARCSLFMNLLPVLTAMLSAIWLQATVDTYFVVGTSLA
                     LGGVMLAQGMQRPLWQRKA"
     gene            1208..1990
                     /locus_tag="VNZ84_05255"
     CDS             1208..1990
                     /locus_tag="VNZ84_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU66396.1"
                     /translation="MPLHDQHKPYELATMPVTGMRADYANGWIIPPHSHRRAQLLYAI
                     RGVMAIDADHGRWIVPPTRGVWIKQGELHSVRMHGDVSMHTLFIDTDAAAGLPTSTCV
                     LDIQPLMKALIVEIVQAGLSYVADSREARIAQLILDEIRLYPALPFHLPWPSSPRLKQ
                     LCEQMTQQLEDAKPSQKWALQLGISDKSFHRHFKQETGITFGQWREQARMLKALEAVA
                     QGDKLLHVALSVGYQSQSAFSAVFKKHFGLSPSAYYQNLPVK"
     gene            complement(2006..3586)
                     /locus_tag="VNZ84_05260"
     CDS             complement(2006..3586)
                     /locus_tag="VNZ84_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HWU66397.1"
                     /translation="MSNSFYFNLILFFLVAIIALEVLANRLRWPPAVAQLAGGALIAL
                     VPELPAFEIDPALVMEVFLPPLLMAGAYFTVWHEFRRHLGSILLLAIGAVLFTTLAVG
                     LALHTFNPGLPWAACFALGAIVSPPDAIAAKAVLQRVALPRKLLVLLEGESLLNDATG
                     LVLYKFSIVALLTGVFSLGGALETFAWLSVGGLLLGAAMGYLVFKTLRYLHGTELMVL
                     ASALPGWICYVVGEHVGVSGVIATVTYGMMLGWHQHEIMSATARRRGVAFWEIMIFVF
                     ESLVFIFIGLSLRGVWERIAHADKSLGAYGLEVLLVLAVVIVSRFVWIFMIDGLARLI
                     RKPHGTHGHSAWGESFVKSWAGMRGVVTLAAALAVPAQVPGRDLMLLCAFAVILVTVV
                     VQGTTIGHVIRAFKLKATTHERIYLNEPQVWARIEQAQYSVVNKLAYNEHGALMHPRL
                     LEQYSYRQSLSDKHKHEDSLPDDIRKAHFNVVLAAVAAGREELLKLHRSGQIHDELLH
                     LMERDLDIQEISALHSRG"
     gene            3881..4126
                     /locus_tag="VNZ84_05265"
     CDS             3881..4126
                     /locus_tag="VNZ84_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66398.1"
                     /translation="MFKLKKWLWTQWQKLRGADRAYAKYLAHFQHYQAQVVNSELQQS
                     LNIQPMSKEAFLQAWQKQGLSSGGKSCGCKTKTGCCG"
     gene            4136..4411
                     /locus_tag="VNZ84_05270"
     CDS             4136..4411
                     /locus_tag="VNZ84_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HWU66399.1"
                     /translation="MEMMRTHSGKLRAIGYDAGKRTLRVTLEDGNTLEYTNVGESLWR
                     DFKNAGAQWSYYRDNIEDTFSCLRVSHQAATSSSSVPARNPLDDLFK"
     gene            complement(4431..5666)
                     /locus_tag="VNZ84_05275"
     CDS             complement(4431..5666)
                     /locus_tag="VNZ84_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU66400.1"
                     /translation="MQGCKLSTDSRQWLAVMNFFMADVRDGLGAFLGVYLLAQSWQAD
                     DIGYVMTIGGLATMLAGIPAGALMDRTHAKRLWLSVCAILMVMGCLVLLLWHSFVAIA
                     LSQAATGVVRAMIGPAIASISLGLVGAKYLPRQLGQNEAWSHAGSLVSVSVAGVLGYF
                     WGLSAVFVLLAVMAVLALMCIRRIRPKEIDYHAARGLAAHAAVSDSGHAVWAALLQSR
                     PLLMLASIMMLFHLANAAMLPLLGQAMVAQGLVNASLFTALTVMIAQCVMIPMALLAG
                     RYAEQFSYERLVKLALLALPLRGVIASSWNSPWAVVPVQLLDGVGAGLLGVALPGLVA
                     KMLQGSGHFNVGLGFVLTIQGLGAALSPAIAGWVAQRYGYALSFTLLTGFAVLALLLW
                     WQQGKQTSAQAAERLQAGH"
     gene            complement(5677..6021)
                     /locus_tag="VNZ84_05280"
     CDS             complement(5677..6021)
                     /locus_tag="VNZ84_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HWU66401.1"
                     /translation="MTAKFAPIVLLLVSNIFMTIAWYGHLKFTGKPLYVVIVVSWLIA
                     LLEYCFAVPANRIGHTVYSVAALKTMQEVISLSVFAVFSVYALGEHLTSSHLLGFALI
                     ALGAFFIFKAPM"
     gene            complement(6131..6949)
                     /locus_tag="VNZ84_05285"
     CDS             complement(6131..6949)
                     /locus_tag="VNZ84_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66402.1"
                     /translation="MLISIPFSSATRWWGTAICVTTFSLAFDVFALPPLPPPIPESST
                     SLPAVSGARVPAAAIQPLDMQATAALPAVAEADVKSILFNPYGEAVGLRLVNDVIVKF
                     PPHRALALLAAVQPGQHVRILGHAEPGVRMGQVMPAGMPAMVKADAIINVTTGNVVVD
                     QPPTPGLPLLPPHLRAQTLQPLQVEGRVELVLNGPRGEMEGVILDNQSIVRFPPESLN
                     TPLHPGMHLVVAGLGSKTASGFSLEAMRMGSSAATMQPMYAPACQAVNTAASQC"
     gene            complement(7105..8445)
                     /locus_tag="VNZ84_05290"
     CDS             complement(7105..8445)
                     /locus_tag="VNZ84_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWU66403.1"
                     /translation="MAYLLIIDDDLAFRSSLSEMLADLGHHVLEACHVQAGLQYLRSE
                     PVDLVITDLKLTGEDGLSFLRQAQAIRGVPCIMLTAYASGSNTIEAMRLGAFDHLTKP
                     VSRQLLMETLQRALPERTGTLAASHAPPQEEESTERLIGNSECMRQVFKQIGLAAGTE
                     ASVLILGETGTGKEEVARALHRNSQRAHGPFVAVNCAAIPPDLMESELFGHIKGAFTG
                     AVADRKGYLRQAEGGTLFLDEIGDMPPALQAKILRVLEAREFSPVGAQTVLSLDARII
                     AATHRDLPAAVRSGQFREDLWYRLQVMPISLPPLRERGEDLLMLAQHFLASEARGRAK
                     RLTAEALKKLVQHDWPGNIRELRNTIARAVIMSHHEWVEAEHISFTPRPQASGLNGGS
                     PPPGGTLAEALQQLEREMIMNALRESGDNRSEAARKLGLSRQQLYRKLDEHGLS"
     gene            complement(8430..9788)
                     /locus_tag="VNZ84_05295"
     CDS             complement(8430..9788)
                     /locus_tag="VNZ84_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU66404.1"
                     /translation="MHTPINTLRRDLIALWSVIAAVSSILVWLLFELSRQGATAQIEQ
                     ARFHAATSCQVMQHGALQAKLLPATTERVNAEGWQAIIDLALREEAGSEGGFWQAGKG
                     VFAYAFPTYDGTGVKRDPPSAELEKITNMAELASHRHQLIVEVRPGLREAIVFAACPL
                     DKMQDAPVAWVMKRILLIQADVLNHLSLALALLIGLVVMSGAWLAWMLGRWQRQVTTL
                     VQQLAQSERMATLGRVSAGLAHEIRNPLATMRMKVENAMAASPERYQQRSQSAFEAVL
                     SQTSRLERLISSLLALTHPLQLDKQVLDLRAWLSGIASLHQEAMVAQQAAWILTVDDE
                     VVEHARTQALLDPEKMAQVMDNLILNALAHVPAGGSIELGLGYTPAQQLRLWVADSGS
                     GVPEHLRSHVFEPFSTARSGGTGLGLALAREIVQAHGGELWLADTPAGACFEIELPWH
                     TS"
     gene            complement(9935..11404)
                     /locus_tag="VNZ84_05300"
     CDS             complement(9935..11404)
                     /locus_tag="VNZ84_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWU66405.1"
                     /translation="MSLISNQIGKSPKAVNHWSARDMYFSLLAPSGFSKGRADSAGFL
                     AAHLKETSQHSSNMPMHPEQLDDWLQDHHQAVGRQFQDYLAARKEGAGRWYFSSKAHA
                     LYFLRTVAPTKMVDGAWLYGITRHWHDSRFASLLKIYLEELGNGVEAMNHVSIYRRLL
                     KVHECEDWKHLDDQFFTQGAVQLALAANTENHIPEVIGFNLGYEQLPLHLLITAYELK
                     ELNIDPYYFTLHTTIDNAHSGHARAAVDAVTEAMPTFGSTRNYMARVMAGVKLNDAGL
                     GTLDIIKHFDLLGEVIRILKDKADIGQYMHANRCLIEGKTINAWLADPDQVARLLYVM
                     QKTGWIKRHHDPEQSRFWQVIAADKAPMFGVFTAYERQVIYDWIAGDVLESLPRVARL
                     GPPPRMSKTMPVAETKQPGNVYDLRSGRRLIEAGLPADDINQNLLSLERYLQGLGQKR
                     MAFLVDWLSPAKHHTSLGLCATRYFKSYLDGAYLQASDT"
     gene            11585..12547
                     /locus_tag="VNZ84_05305"
     CDS             11585..12547
                     /locus_tag="VNZ84_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU66406.1"
                     /translation="MKKSVKTLFQPHIDDALVSVLDALQARNYRFVTVTPVTHHLVNQ
                     RPENAWSHSLQDIFGWNRPFMASTLDAELFELMQAANIARPLAQGWQSLLRVSSLNNT
                     LFAHSAFPTDAEDAVFFGPDTYRFIRAMDHALPGLQHPIQRCVDIGTGPGTAAILLAD
                     RFPEAEVWGVDINPKALNLAASNAKAKGISNVHFEYSNLLNDIAGNFDLIVANPPYLV
                     DPFERAYRHGNGPLGAQLALDIVDTALARLTPGGTLMLYTGVAMLDGQDPFLDAVEEK
                     INKAGASYQYSEIDPDIFSEELLNTAYIEADRIAAVWLQVTRPM"
BASE COUNT         3277 a         3644 c         3143 g         2642 t
ORIGIN      
        1 ggtacatggc atccatgtca tgtgtatgct acgccggcga tgccggactg gcaatacaca
       61 aaaaacagtg tccatcaagg cttcaggcga aatattttta gcgcgcagtt tttaatcacc
      121 cccattttaa atcgtcaaat catagggtta cagcactcac ccacaacaca acccaaaagt
      181 tatccacaaa aaatgtggac agcgcatcta ctcctatccc ttacgctttc cgctgccaca
      241 gcgggcgttg catcccttgc gccagcatga ccccgcctaa tgccagactg gtgccaacca
      301 caaagtaagt atctaccgtc gcctgcagcc aaatagcaga aagcatggcg gtcaggacag
      361 ggagcagatt cataaacaaa ctgcatcgtg ccgggcctat caatagcacg cccttaatcc
      421 agaaataagg caacagtacc gatgccagca cgccggcata cagaatgagc ggcaatgtat
      481 gcgcattcac gttgatggct ggccgggtaa acaacaaagc gacccccata caaagacaaa
      541 cacacagggc ctgcatgaaa gtcgattgcc aggcagacag tgggaatgcc catttacgaa
      601 tcaaaacact ataaagcgcg tacaccagag aagccatcag catcaataca tctccttgcg
      661 gcgcgccctc ttgcaccagg gacattggtt gaccatggct aatcaggatg gtaattccga
      721 gcaaggcagt caatccgccc gaaatcatgc ccacactgat ggcctcccct aacaggcccg
      781 cactcaccag catggtcagc aaaggattta aagccgtcat cacggccata tgattcgccg
      841 tggtcgtcaa cgctgcctga tatgacaaac actgataaaa agccatgctc aaaaaaccta
      901 atactgccag cttgggcaag gattgaatga tgacagcccg gttgcgccag atggaaggca
      961 acaggaataa tgccattaca atcaaagcga ccagcaaacg gcaaaatgtc atcgcaattg
     1021 ggtgaatcaa accggcagct aacttggaaa ccaaaacatt cccggaccac atcatgatgg
     1081 ccagcaaggg aaaagtaaca ctcttcatcg tcagattcct gtgtcgttga aaaaagacat
     1141 ggtaaagtgc aaggcaacta ctatctttag acaaacagcc aacttttatc taaaaatgga
     1201 ccaaacaatg cccttgcatg atcagcacaa accctacgaa ctggcgacca tgccagtcac
     1261 gggcatgcgt gccgattacg ccaatggctg gatcatcccg ccacacagcc atcgccgggc
     1321 gcaattgcta tatgccattc gaggggtgat ggccatagat gcggatcatg gccgctggat
     1381 tgtgccgcct acgcgcggcg tatggatcaa acaaggtgaa ttacattcgg tgcgcatgca
     1441 tggggatgtc agtatgcata cgctgtttat tgatacagat gcggcggctg gattgcccac
     1501 cagcacctgt gtgctggata tccagccact gatgaaggca ttgattgtag aaattgtcca
     1561 agccggtcta agttatgtcg ctgactcccg cgaagcgagg attgcacagt tgatcctgga
     1621 tgaaatccgg ttatatccag ccttgccttt tcacttgccc tggcccagct cacccaggct
     1681 caaacagctc tgtgagcaga tgacgcaaca gctggaggat gccaaaccct cacaaaaatg
     1741 ggcactacag ctcggcatca gcgacaagtc atttcatcgg catttcaaac aggagacagg
     1801 cattaccttt ggccaatggc gggaacaggc gcgtatgctc aaagccctgg aagcagtcgc
     1861 tcaaggagac aaattactcc atgtcgccct gtcggttggc taccagagtc aaagcgcttt
     1921 ttcggccgtg tttaaaaagc attttggcct cagcccatcg gcctactatc aaaacctgcc
     1981 tgtgaaataa gggaccatac cgatactagc ctcggctatg taatgcggaa atttcctgga
     2041 tatcgaggtc gcgttccatc aggtgcagga gctcatcatg aatctgcccg ctacgatgca
     2101 gtttaagcag ctcttcgcgg ccagcggcca ctgccgccag taccacgttg aaatgtgctt
     2161 tgcggatatc atcaggcaga ctatcttcat gcttgtgttt gtctgacagg ctttgccggt
     2221 aactgtattg ctccagcaaa cgcgggtgca tcaatgcacc atgttcgtta taggccagtt
     2281 tgttcaccac gctatattgc gcttgctcaa tccgcgccca tacttgcggc tcattcaggt
     2341 aaatgcgctc atgcgtggtc gctttcagtt taaatgccct gatcacgtgt ccaatcgtcg
     2401 tcccctgtac caccactgtc accaggatca cagcgaatgc acacaacagc atcaagtcgc
     2461 gccccggcac ctgggcgggc accgccagtg ccgctgccag cgtaaccacc ccgcgcatac
     2521 cggcccagct tttgacaaag gactcgcccc acgcgctatg cccatgcgta ccatgcggct
     2581 tgcggatgag cctggccaaa ccatcgatca taaaaatcca gacaaagcgt gacacgatca
     2641 ccaccgcaag caccagcaac acctccagcc catacgcacc cagtgatttg tcggcatggg
     2701 cgatacgctc ccatacgcca cgcaaggaca ggccgataaa gataaacacc agcgattcaa
     2761 aaacaaaaat catgatttcc caaaatgcga ctccgcggcg gcgcgcagtg gcagacatga
     2821 tttcatgctg atgccagccc agcatcatgc cgtaagtcac tgtggcgatc acacctgaga
     2881 cgccgacatg ctcgcctacc acataacata tccatcctgg caaggctgag gccagcacca
     2941 tgagttcggt gccgtgcagg tagcgcaggg ttttaaaaac caggtaaccc atagccgccc
     3001 ctaacaacag tccgccgaca ctcagccagg caaatgtttc gagcgcacca cctaaagaaa
     3061 atacgcccgt cagcaaggcg acaatggaaa acttgtacag caccaggcca gttgcgtcat
     3121 tgagcaggct ctccccttcg agcaatacca gcagcttgcg tggcaatgcc acgcgttgca
     3181 gcaccgcctt ggcggcaatc gcatccggcg gcgacacaat cgcccccaag gcaaaacagg
     3241 ctgcccacgg caggccgggg ttgaacgtat gcaaggccag ccccaccgcc agtgtggtga
     3301 acagcacagc gccaatcgcc agcagtaaaa tactgcccag gtgacgcctg aattcatgcc
     3361 aaacggtgaa ataggcgccc gccatcagca agggcggcaa aaaaacctcc atcacgagtg
     3421 ctggatcgat ttcaaaagcg ggtaactccg gcaccaaggc gatcagcgcc ccgccagcca
     3481 gttgcgcgac tgcaggtggc cagcgcaacc ggttggccaa gacctccagt gcaatgatgg
     3541 ccaccagaaa aaacaggatc aaattgaaat aaaacgaatt actcatgatg gatgaagagg
     3601 agcggcgccc cattaaacca gattaaacaa tgccctgtgg acacgtaagc agccataatc
     3661 taacacaggc agcttgcgcc aagcttgctt taacaatgtt tgatacacac cgatacgaga
     3721 ggcataaaac cagacttgcc acgctggcac caaactggaa cttgccagcc agccagtgtt
     3781 cataactgtt atgccctgta ttaaacacct cactacagcc acggccagct ccaagttgga
     3841 gtaagatgac ggtagtcagg tgcgggataa aggagtcaac atgtttaaac tgaaaaaatg
     3901 gttatggaca caatggcaga aactgcgcgg ggcagatcgt gcctacgcta agtacctggc
     3961 acatttccag cattaccagg cccaagtggt gaatagcgaa ttacagcagt ctttgaacat
     4021 ccagcccatg agcaaagagg ccttcctgca agcctggcaa aaacaaggcc tcagcagcgg
     4081 aggaaaatcc tgtggttgca agaccaagac tggatgctgc ggctaggata atgtcatgga
     4141 aatgatgcgc acccattctg gcaaactacg cgcaattggc tatgatgcag gcaaacgaac
     4201 tttgcgcgtg acattagaag acggcaacac actcgaatat accaatgtgg gcgaaagcct
     4261 gtggcgtgac ttcaaaaacg ctggggctca atggagctac tatcgtgaca acattgaaga
     4321 cacctttagt tgcctacgcg tgagccacca agctgccacg tcttcctcct cagtcccggc
     4381 ccgcaatccc ctggatgatt tatttaaatg aagatgcatg gaaataccca ttaatgtccg
     4441 gcttgcaagc gctcggccgc ttgcgctgat gtttgtttac cttgttgcca ccacagcaac
     4501 agggccagca ccgcaaaacc tgtcagcagg gtaaaactca acgcatagcc ataacgctgt
     4561 gcaacccaac ccgcaattgc agggctcaag gcggcaccca gcccctgaat agtcagcaca
     4621 aagccgaggc cgacattaaa atgcccgctc ccctgcaaca tcttggccac cagcccaggc
     4681 aaggccacac ccagcaaccc tgcgcccacg ccatccagta attgcacagg caccaccgcc
     4741 cagggcgaat tccagcttga agcgattaca ccgcgtagcg gcaatgcaag cagggcgagc
     4801 ttgactaggc gttcgtagct aaattgctcg gcataccgcc cggccagtaa ggccataggg
     4861 atcatgacac actgtgcaat catcaccgtc agggcagtaa acaggctagc attgaccaaa
     4921 ccctgcgcaa ccatggcttg cccaagtaaa ggcagcatgg cggcatttgc cagatgaaac
     4981 agcatcataa tggaggccag cattaacagg gggcgggact gtagcaaagc agcccacacg
     5041 gcatggccag aatcactcac cgcggcatgt gccgccagtc cacgtgccgc gtggtaatca
     5101 atttctttcg ggcggatacg gcgtatgcac atcagcgcca gcaccgccat gaccgccaac
     5161 aggacaaaga cagcagacag gccccagaaa tagcccaaca ccccagccac ggagactgac
     5221 accagactgc cggcatggct ccaggcctca ttctggccga gttgcctggg taaatatttt
     5281 gccccaacca atccaaggct gatactggca attgccggcc ctatcatggc gcgtaccacg
     5341 ccggtcgcgg cttgtgacaa ggcaatggcc acaaaagaat gccacagcaa cagcaccagg
     5401 caccccatca ccatcaggat ggcgcacaca gacaaccaca ggcgcttggc atgggtacgg
     5461 tccatcagcg caccagccgg gatgccggcc aacatggtgg ccaaaccacc tatcgtcatg
     5521 acatagccga tatcatcagc ctgccaggat tgtgccaata aatacacccc gagaaaggca
     5581 cccagcccat cgcgcacatc ggccatgaaa aaattcatga ctgccagcca ttgtcgggaa
     5641 tccgttgata atttgcagcc ttgcatgcgc ctctccttac atgggtgctt taaagataaa
     5701 aaaagcgccc aatgcaatca aggcaaagcc cagtagatga ctggaggtga gatgttcgcc
     5761 tagcgcatat acggaaaaca ccgcaaacac gctgagcgaa atcacttcct gcatggtttt
     5821 aagggcagcc accgaataca ccgtatgacc gatacgattg gctgggacgg cgaaacaata
     5881 ttcgagcagc gcaatcagcc agctcaccac aatcaccacg tacagcggct tacccgtgaa
     5941 cttgaggtga ccataccagg cgatcgtcat aaaaatatta gagaccagta gcaacactat
     6001 gggggcgaat tttgcagtca tgctgaatcc tatcgggaga aataagcagg tttgacctgg
     6061 ggtgataagg gcaacaacat tgcccatgcc aactcaaatg ccaggtcatg cccaatggtt
     6121 aatttttggc ctagcactga ctggccgcag tgtttaccgc ctggcatgcc ggtgcgtaca
     6181 tgggctgcat tgtggcggca gaactcccca tacgcatggc ctctaaagaa aaaccactgg
     6241 cagtttttga gcctaagcct gccaccacaa gatgcatgcc gggatgtaat ggcgtattca
     6301 ggctttccgg ggggaaacga acaatactct ggttatccag gatgacgcct tccatctcac
     6361 cacgagggcc atttaagacg agctctaccc ggccttctac ttgcaatggc tgcaatgtct
     6421 gtgcacgcaa atgtgggggc aacaatggca ggccaggggt tggcggctga tccaccacaa
     6481 cattgccagt ggtcacattg ataatggcat cagctttcac cattgctggc attccagctg
     6541 gcatgacctg tcccatacgc actcctggtt cagcatgccc cagaatgcgt acatgctggc
     6601 ctggttgcac cgcagccagc aatgccagag cgcggtgcgg tgggaatttg acaatcacat
     6661 cattgaccag gcgcaacccg acggcttcgc catacggatt aaacaggatg cttttgacat
     6721 cagcttctgc cacggccggt aatgccgccg tggcttgcat gtctaatggt tggatggctg
     6781 cagctggcac ccgtgcccca gaaacagcgg gcagagacgt cgaagattca ggtatgggtg
     6841 gcggaagcgg gggtaaggca aatacgtcaa aggcaagact gaaagtagtg acgcaaatag
     6901 cggtgcccca ccagcgagta gcgcttgaaa aagggataga aattaacatg agatgctcct
     6961 tgtcagtgat gaacctgatt catctttgca aggggcatgc caaataaaaa attttttaat
     7021 atcaacaagt taaaaaaatt taacctgtca aaccgtccgt tctggtgaca gtcttctgtc
     7081 cgcaaggcgg acaggtaggc agacttaact caaaccgtgc tcatcgagtt tgcggtagag
     7141 ctgctgacgc gacaacccga gtttccgcgc cgcctcacta cgattatctc cactctccct
     7201 gagtgcattc atgatcattt cgcgttcgag ctgctgtaat gcctcagcca aggtgccgcc
     7261 aggaggcggt gacccgccat tcaaccctga agcttgggga cgaggggtaa atgaaatatg
     7321 ttcagcctct acccactcgt gatgactcat aatcactgca cgggcgatcg tattgcgtaa
     7381 ctcccggata ttaccgggcc aatcatgctg aacgagcttt tttaaggctt cagccgtcag
     7441 tcgcttggcg cgaccacgcg cttcgctagc gaggaaatgt tgtgccagca taagcagatc
     7501 ttcgccacgc tcccgcagcg gtggcaatga gatcggcatc acctgtagcc ggtaccataa
     7561 atcttcacga aattgcccgc tacggacagc agcgggcagg tctctgtgtg tcgcggcgat
     7621 gatacgcgca tctagcgaaa ggactgtttg cgcgcccaca gggctaaact cgcgcgcctc
     7681 caatacccgc aaaatcttgg cttgcaacgc cggcggcatg tcgccaatct catcgagaaa
     7741 cagggtgcct ccttcggctt ggcgcaaata acctttgcga tcggcaaccg caccggtaaa
     7801 tgcgcctttt atatgcccaa acaactcgct ttccatcaag tcgggaggaa tcgccgcgca
     7861 attgacggcg acaaaagggc catgggctcg ctggctgtta cgatgcaggg cacgtgccac
     7921 ttcttctttg ccagtgccgg tctcgcccag gatcaggacg cttgcctccg tgcccgccgc
     7981 cagaccgatt tgcttgaaca cttgccgcat gcattcacta ttcccaatca agcgttctgt
     8041 gctctcctct tcctggggtg gagcatggct agccgccaag gtgcccgtcc gttcaggcaa
     8101 tgctcgctgc aaggtttcca tcagcaattg acgcgaaacc ggcttggtta aatggtcaaa
     8161 ggcgcctaaa cgcatggctt ctatcgtgtt gctgccactc gcataggctg tcaacatgat
     8221 gcagggcacg cccctgatcg cctgcgcctg gcgtaaaaaa gacaggccat cttcgcccgt
     8281 gagtttgaga tcggtgataa ccaggtctac aggctcagac cgcaagtatt gcaggccagc
     8341 ctgcacatgg cacgcttcga gtacatgatg tcccagatcg gccagcattt cagacaggct
     8401 gcttctaaac gccagatcgt cgtcaataat caggaggtat gccatggtaa ctcaatctca
     8461 aaacaggcac ctgcaggtgt atcagccaac cacaactcgc ccccatgcgc ctggacaatc
     8521 tcacgtgcaa gggcgagtcc caagccggta ccgccgctac gtgccgtact aaacggttca
     8581 aatacatggc tgcgcaaatg ttcaggcacg ccactgccac tgtctgccac ccacaatcgc
     8641 aattgctgag cgggtgtata cccaagccct aactcaatac ttccgccagc cggcacatgt
     8701 gccaaggcat tcaggatcag gttatccatg acctgcgcca ttttttcagg atccagcaag
     8761 gcctgtgttc gggcatgctc aaccacctcg tcatctaccg tcaatatcca ggctgcctgt
     8821 tgcgcgacca tggcttcttg atggagactg gcgatcccgg acaaccaggc acgtaaatcc
     8881 aacacctgct tatcaagctg aagcggatga gtaagcgcta acaaactgga tatcaggcgt
     8941 tcgagacggg aggtttgcga taatacggcc tcaaaagcac tttgactgcg ttgctgataa
     9001 cgctcgggcg aggcggccat ggcattctcg accttcatgc gcatggtggc caagggatta
     9061 cggatttcat gtgccagccc tgcagatacg cggccaaggg ttgccatacg ctcggattgt
     9121 gcaagctgtt gtaccaaggt cgtcacctgt cgttgccagc gtcccaacat ccatgccagc
     9181 caggcgccag acatcaccac cagcccaatc agcaaagcga gcgctaaact taagtggttc
     9241 agcacatccg cctgaatcag taatatcctt ttcatcaccc aggccacggg cgcgtcctgc
     9301 atcttatcca gagggcaagc cgcaaagaca atagcctccc ttaacccagg ccgtacttcc
     9361 acaatcagtt gatggcgatg gctggccaat tcagccatat ttgtgatctt ttcaagctcg
     9421 gcactgggcg gatctcgctt aaccccagtg ccatcatacg ttggaaaagc ataggcaaac
     9481 acgcctttgc ctgcttgcca gaaaccgcct tcactcccag cctcttcgcg caaggccaaa
     9541 tctatgatgg cctgccaacc ctctgcattg acgcgctcag tagtggctgg caggagtttg
     9601 gcctgcagtg ccccatgttg catgacctgg caactggttg cggcatggaa gcgggcctgc
     9661 tcgatttgtg cggtggcacc ctggcggctt aattcgaata acaaccagac caggatggag
     9721 gagaccgctg cgatcacaga ccacagcgcg atcaagtcgc gacgcaaagt attgatgggt
     9781 gtatgcataa gcgctattta aatcatgaaa ataggcatct gccatggcga ggtaagctca
     9841 tacctgcaag gtagcataac accaccagcg cttcaatcca gcgcccttgg taggcattat
     9901 cgccgatacg acgcttgtca ttgcacctac acgtctatgt atcggaggcc tgcaagtaag
     9961 cgccatccag ataagattta aaataccgcg tggcacacaa cccaagagag gtgtgatgct
    10021 tggcagggga cagccaatcc accaggaacg ccatacgctt ttgccccagg ccttgcaggt
    10081 aacgttccag cgacaataga ttctggttaa tgtcatcagc aggcaggcct gcttcaatca
    10141 ggcgacgccc ggagcgcaag tcatatacat tccccggctg cttagtctca gccactggca
    10201 tggttttact catccgcggt ggcgggccta accgggccac ccttggcagg ctctccaata
    10261 catcaccggc aatccagtca tatatcacct gccgctcata ggctgtaaac acaccaaaca
    10321 taggggcttt atctgccgca attacttgcc agaaacggct ttgctcaggg tcatggtggc
    10381 gtttaatcca gccggttttt tgcatcacat acagcaacct ggccacctgg tcaggatccg
    10441 ccaaccacgc attaatggtt ttaccctcaa tcaggcagcg attagcgtgc atatactgcc
    10501 caatatcagc cttgtctttc aggatacgga tcacttcacc gagcaaatca aagtgcttga
    10561 taatatccag tgtacccagc cccgcatcat ttaatttcac gccagccatg acccgcgcca
    10621 tgtagttgcg agtcgagcca aaggtgggca tcgcttcagt gacagcatcc accgctgcac
    10681 gcgcatgccc gctgtgggca ttatcaatgg tggtgtgcag cgtaaaataa tacggatcaa
    10741 tattcagctc tttcagctca taagccgtaa tcagcaaatg taggggcaat tgctcatagc
    10801 ccaggttaaa gccaatcact tccgggatat ggttctcggt attggcggcc aatgccaact
    10861 gaactgcacc ttgcgtaaag aactggtcat ccaggtgttt ccaatcttcg cactcatgga
    10921 ctttaagcag cctgcggtaa atagagacat ggttcattgc ctcaacccca ttccccagct
    10981 cttccaggta gatcttcagc aatgaggcaa accgtgaatc atgccaatgc cgggtgatgc
    11041 catacagcca ggcaccatcg accattttgg tcggcgccac cgtgcgcaga aaatacaatg
    11101 cgtgcgcttt gctgctaaag taccagcgac ctgccccttc tttcctcgcc gcaaggtaat
    11161 cctggaactg gcggccaaca gcttgatggt gatcttgcag ccagtcatcc agttgctctg
    11221 gatgcatagg catattgctt gagtgttggc tggtttcctt gagatgggca gcgaggaagc
    11281 ccgcactatc tgccctgcct ttgctaaaac cagagggggc aagcagtgaa aaatacatat
    11341 ccctcgctga ccagtgattc accgctttcg ggctttttcc gatttgattg ctgatgagtg
    11401 acatctcttc tgcctccgat gattaacttg cagagttaat tgctagtaga aagcagataa
    11461 gttacgcaga tgtgatcaaa atccctatca gacgatgcag gagcatgctg taggaatgcg
    11521 cttataagag aaaacgctgg cttctgctaa gctggtagac accgtttcgc tggaggtttc
    11581 ttctgtgaaa aagtctgtaa agaccttatt tcaaccacat attgatgacg cattggtcag
    11641 tgtgttggat gccttgcagg cccgcaatta ccgttttgtc acggtcacgc ccgtcacgca
    11701 ccatctggtc aaccagcgtc cggaaaatgc ctggtctcat tctttacagg acatttttgg
    11761 ctggaatcgc ccatttatgg ccagcacgct ggatgcggag ttatttgagc tgatgcaagc
    11821 tgccaatatt gcacgcccac tggcccaggg ctggcagtca ctcttacgtg tatccagtct
    11881 caataacacc ctgttcgcgc actccgcttt tcccactgac gccgaggacg ccgtcttttt
    11941 tggcccggac acttaccgtt tcatacgcgc catggatcac gccttgccag gcctgcaaca
    12001 tcccatccag cgctgtgtgg atatcggcac cggaccaggg actgccgcca ttttactggc
    12061 cgacagattc cctgaggccg aggtgtgggg cgtggatatc aaccccaagg cactcaacct
    12121 ggccgccagt aatgccaagg cgaaaggcat cagcaatgtc catttcgagt acagcaacct
    12181 gctcaacgat attgcgggta attttgacct gattgtggct aacccgcctt acctggtcga
    12241 cccgtttgaa cgtgcatacc gccatggcaa tgggccatta ggtgcacaac ttgcgcttga
    12301 tatcgtcgac accgcgctgg cacggctgac cccaggtggg accttgatgt tgtataccgg
    12361 ggtcgccatg ctggatgggc aagacccatt tcttgatgcg gtcgaagaaa aaatcaacaa
    12421 ggcaggggca agctaccaat attccgaaat tgaccctgac attttcagcg aagagctgtt
    12481 gaacactgcc tatattgaag cggacaggat agccgcggta tggctacaag tgactcgacc
    12541 gatgtgatgg acaacccttg ctgacagcaa gctgacaact gccatatttg ctgcacatgc
    12601 agcataaaca tcacggtagt atgcaatcac ctgctgccgc atcacctgac tggacaactg
    12661 tatgaccaac aatgaccgtc ttgtgtatca acattttgga ctaggt
//
LOCUS       DATMSK010000158        11861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_106074,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000158 DATMSK010000000
VERSION     DATMSK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11861
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106074"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1596)
                     /locus_tag="VNZ84_05310"
     CDS             complement(139..1596)
                     /locus_tag="VNZ84_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HWU66407.1"
                     /translation="MRIEWSQNSEVNSTLLSAFSKHVLVVFEEALLSSLPFYEVLTSK
                     LQRAGLTTEDLKQKPVTLEMAEGTLISVCQLEAKASTFTMNTLLRAAVKPLLSEHPER
                     IALAVYSKKQASLFARLAAYVTLVNAQALPVLKQKPPGPKLREIGLFGVKDLDWTNEV
                     TALVAGNTVCRALTMTPPNMLTPALYRQCLQTMAHEKGWQYTEWDTEALKHLGAGAFV
                     AVAQGSDPQDAAIVKLSYRHPQAQKTLALVGKGICFDTGGHNLKSSKYMQGMHQDMNG
                     SAVAIGVLQAISAQQLAINVDCWLALAQNHIGPNAYKQNDVITALNGMTIEIVHTDAE
                     GRMVLADTLTLASRERPEVMIDYATLTGSMQTALGSRMSGIVANREPLAQQMIEAGAL
                     SGERVVPFPYADDYDSELESKIADIKQCTLDSDADHILAARFLGKFIEGEVDWCHVDL
                     SSYAHKEGLGAVASEVNGFGVALTLQWLQAFMAGK"
     gene            1896..4565
                     /gene="aceE"
                     /locus_tag="VNZ84_05315"
     CDS             1896..4565
                     /gene="aceE"
                     /locus_tag="VNZ84_05315"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HWU66408.1"
                     /translation="MATHSQAHLNETDAQETQEWLEALSAVIENEGPERAHFLLEAMI
                     DQARRSGGHLPYKATTAYVNTIPTHLQAKLQGDPEMERRIRALIRWNAIMTVLRANEK
                     SPGIGGHIASFQSAATLYDVGFNHFFRAANESFGGDCIYFQGHSSPGVYARAFLEGRI
                     TEEQMDNFRQETGGNGLSSYPHPWLMPDFWQFPTVSMGLGPLAGIYQARFLKYLHNRG
                     IADTADRKVWVFCGDGEMDEPESQGAISLAAREGLDNLIFVINCNLQRLDGPVRGNGK
                     IIQELESNFRGAGWNAIKVIWGSYWDPLLAMDKDGLLRKRMEECVDGEYQNFKQKGGA
                     YTREHFFGKYPELREMVAAMSDADIWRLNRGGHDPHKVFAAYQSAVNHKGQPTVILAK
                     TVKGYGMGEAGEGQNTTHQQKSMDIASLKAFRDRFDLPLTDEQVENLSYYRPAEDSPE
                     IKYMMARRAALGGFVPARRRKGNELTVPELSAFENMLGATGEREISTTMAFVRILSTL
                     VRDKQVGKYVVPIVPDEARTFGMEGMFRQLGIYSSVGQLYEPQDSDQVMFYKESQSGQ
                     ILEEGINEAGSFASWLAAATSYSVTGTQMIPFYIYYSMFGFQRIGDFAWAAGDSRARG
                     FLLGATAGRTTLNGEGLQHEDGHSHLMSATIPNCISYDPTFAYELAVIIQEGMRRMVQ
                     NQEDVYYYITLMNENYTHPAMPEGSAEGILKGMYSFSKSKAKGPKVQLMGSGVILREV
                     IAAAELLEKDWGVSADVWSVTSFTELRRDGIDAERHNLLNPEAKPKLSYVAQALAKAE
                     GPVIASTDYMRSFADQIHNYVPNRFVALGTDGYGRSDSREALRSFFEVDRYYVVLAAL
                     KALADDGKLPASKATEAIKKYNIDITRANPATI"
     gene            4592..5935
                     /gene="aceF"
                     /locus_tag="VNZ84_05320"
     CDS             4592..5935
                     /gene="aceF"
                     /locus_tag="VNZ84_05320"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HWU66409.1"
                     /translation="MSIKEVLVPDIGNFDSVDVIEVLVSVGDTVAKEDSLITVESDKA
                     SMDIPSSLSGVVKEIHIKVGDKTKQGALILTLDTAGEAAPAEKVAPQPTATPVVATVT
                     VDEAKVAIPEPTRPVAEVPQVIQPQATPNPVAASTVASDGKLAHASPSVRKFARELGV
                     NLSLVKASGPKNRILQSDVQAYVKGELSKPRSENMGVGTGLSTLPMPVIDFSQFGTIE
                     TKPLSRIKKLSGANLHRNWVTAPHVTQFDEADITDLEDFRKSMLAEAEKRGVKLTLLA
                     FLMKAVVNALRTYPNFNASLSPEGDSLIMKQYYNIGFACDTPDGLVVPVVRDVNQKDV
                     MDIARDLADLSAKARERKLKVEEMQGGCFTISSLGGIGGTMFTPIINCPEVAILGVSR
                     AAMQPVYNKDSGGFDPRLIMPLSLSYDHRVIDGADGARFTSHLRVMLSDVRRLLL"
     gene            complement(5981..6391)
                     /locus_tag="VNZ84_05325"
     CDS             complement(5981..6391)
                     /locus_tag="VNZ84_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HWU66410.1"
                     /translation="MSDLLFVYGTLRQGNTNDMATYLANHAVFVAGGWFQGQMYQISY
                     YPGVIASDNPGDRVYGEVYRLHDARGTLAILDDYEECGALDEQPAEYQRVQTQVLVMD
                     GVILKPVWIYLYQWSLVNKAIIVTGDFMQQNQLV"
     gene            complement(6397..7485)
                     /locus_tag="VNZ84_05330"
     CDS             complement(6397..7485)
                     /locus_tag="VNZ84_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HWU66411.1"
                     /translation="MNEKPLIYKGRAASSNLVSRFDPHTRESLDDGWGGLDEEAPPLR
                     TEVMLDVSRSVINYIQSPDLPFDRTLNPYRGCEHGCIYCYARPTHAYLGMSPGLDFES
                     RLLMKADAVALLKKELAHPKYQCKPIALGMNTDGYQPIEREYQLTRQIIQVLSEANHP
                     FSLVTKSSLVERDIDLIAPMAAKRLASIYVSITTLDRQVARRLEPRAAAPERRFETLR
                     KLSEAGIPTGVMVAPVIPALTDCDMEQILEKAHQAGARKAGYVFLCLPHELSDLFEQW
                     LQQSFPLKANHVMSIVRQSRGGKSYQSGFGQRMRGEGVFADLLSTRFKLSCQKLGMSG
                     ERLGFRTDLFKVPDAWRQSSSHPQLVLF"
     gene            complement(7581..10142)
                     /locus_tag="VNZ84_05335"
     CDS             complement(7581..10142)
                     /locus_tag="VNZ84_05335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU66412.1"
                     /translation="MIVSWFKKLIPGLVFAILFSALHFGLWALTNRALPLINVKPTVN
                     GFAYTGYRANQSPLEGQHPSTAELAEDLDLMHKHTRHIRMYSSLDQNEVIPLAARRNM
                     NVIAGAWIDTDNMKNTREVSALLEKLENYSNIDRVIVGNEALLRNDLPVQALIGYLDF
                     VREHSNVPVSTAEPWHVWIKHPELVKHVDYIAVHLLPYHEGVPVEKAVQYAIERYNQL
                     MEAYPRKKIVITEVGWPSRGPAIGASVASEVNQARFIREFLARNSIEDLDFYLMEAFD
                     QPWKVALEGWAGAYWGMYNADRHQKYSLQGAVPPNTRWIAKATWSTALAFIPLMLIAW
                     RFKHWGIGGVLSMAVLFQACITTLTIAWNLPGDYYYTLRDFVVLVGLILGIALTSMVL
                     MIYAAEFSEVMFKPYWRRLFKRAQPLPADQELFVSIHLACYNEPPEMVIATIDSLRNL
                     NYTRYEVIVVDNNTKDEAKWKPLEAYMANMPDNFHFYHLPSWPGFKAGALNFALDKTH
                     PDAQVVGVVDADYVVTPDWLSDLVPHFLESQVAVVQAPQAHREWEHSFFHRMCNWEFE
                     GFFRIGMHHRHERNALIQHGTMTLIRAESLKGFGGWSEWCICEDTELGLRLLENNMEL
                     RYIDETFGRGLTPANFKALKSQRNRWAFGAMQILKHHMPKLLGKSSLNFSQRYHFLTG
                     WFGWFGEALQLLFTIGSIGWTIAMLAFPKYFSLPVVVMIVPILCFLAIKAILGPVLYR
                     KTMHCSWMDIFGASLASLGLSHAIARGIISGLTHKAGVFVVTNKTKAKLSSWQLIAPI
                     KEELTILISLLLCAVAMIYSRGFSNLDAQLWVSMLALQSLPYWSALACQWISEKR"
     gene            complement(10190..11764)
                     /locus_tag="VNZ84_05340"
     CDS             complement(10190..11764)
                     /locus_tag="VNZ84_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66413.1"
                     /translation="MIILQRLLSRVSPVVWLTLFLIGSLYYVWRLQQPQVLIDAATDA
                     LPLQCVSYAPYYKPGMNPTVKDSFVDPVQIEQDLQALSKVTKCVRTYSVSQGLDYVPK
                     AAQKLGMTVILGVWIGWVDDLNRKELQLAVQQANQYPDTVRGLVVGNEVLLRGEQSEP
                     KMHEYLQWAKANTKAPITYADVWEFWLKHPTLEQDVDFVTVHILPYWEDKPVAIEQAV
                     AHTASVMGHLGTVFKKPILIGETGWPSQGRQRFWSKPSAINQALYMRGFLNAAQQNHW
                     QYNVIEAVDQPWKRELEGTVGGYWGVLDTGLQPKFSLQGSMAERSDGFKPYLVALVLA
                     VLGAAWGQYTQLSARYKVLLALTGLAAGLHAYLQVDYVHMAARNTGEYVMLGGLVVLG
                     WALAWLQLRQWLGLKVYSCIEQSLVRLLALAFLLTSAGLAINGRYLDFPLILVCLPLV
                     LITISLISSTKIEQSAAAFNQAGFWLSAVMGVMASNMAVAVAMMEPGNIMAWWWCGAC
                     GLALFVLPARSLQLSR"
BASE COUNT         3009 a         3214 c         2918 g         2720 t
ORIGIN      
        1 tgaaaggccg tgaatggctg gcccgctgcg gggcttgcgg ttaaggctaa ccaagacttt
       61 ccatgaggcg attttcaatc tacttgaagg tccgcttgtt cagcgttgtc gtttcaactc
      121 ctcatggaat atttcgattt atttgcctgc cataaaggcc tgtagccatt gcaaggtgag
      181 cgccacacca aatccgttca cctcgctggc cactgcgccc aggccttcct tgtgcgcata
      241 ggaagataag tctacatggc accagtccac ctcgccttca ataaacttgc ctagaaaacg
      301 tgccgccagg atatggtcgg catcgctatc cagggtgcat tgcttgatat cggcaatctt
      361 gctctctaat tcactgtcat aatcgtcggc atacgggaaa ggcaccacgc gctcaccact
      421 gagcgccccc gcttctatca tttgttgtgc taatggctca cgattagcca caatcccgct
      481 catacgcgaa cctaaggcgg tttgcatact gccagtcaat gtcgcgtaat caatcatcac
      541 ttccggtctt tcacggctgg ccagtgtcag cgtatccgcc agcaccatac gtccttcagc
      601 atcggtgtgg acaatttcta tcgtcatgcc attgagggcg gtaatcacat cattttgctt
      661 ataagcatta gggccaatat ggttttgcgc cagcgccagc cagcaatcaa cgttaatcgc
      721 caactgctgt gcgctaatcg cctgcaacac gccaatagcc accgcgctac cattcatgtc
      781 ctgatgcatg ccttgcatat atttggacga ctttaaattg tggccgccag tatcaaagca
      841 aatcccctta cccaccagcg ccagcgtttt ctgtgcttgc gggtgacggt aggagagttt
      901 gacgatggcg gcatcttgcg gatctgagcc ctgtgccacg gccacaaaag cccctgcacc
      961 caagtgcttc agcgcctccg tgtcccattc ggtatattgc cagccttttt cgtgtgccat
     1021 ggtttgcagg cattggcgat acagcgcagg cgtcagcata ttgggtggcg tcatggtcaa
     1081 tgcacggcag acagtattgc ccgcaaccaa ggccgtgact tcatttgtcc aatcaaggtc
     1141 tttaacccca aacagaccga tttcacgcaa ttttggcccg ggtggttttt gttttaatac
     1201 cggcaatgct tgtgcattca ccaaggtgac ataggcggcc aatcgggcaa ataatgacgc
     1261 ctgctttttt gaatagaccg ccagcgcaat acgctccgga tgctccgaca gaagtggttt
     1321 aacagccgcg cgcaataagg tattcattgt gaatgtggat gcttttgcct caagctggca
     1381 cacgctgatc aaagttcctt ccgccatttc cagggtcaca ggcttttgct ttaaatcctc
     1441 agtcgtcagg cctgcacgtt gtaatttgct ggtgagtacc tcgtaaaatg gcaatgacga
     1501 caataacgcc tcttcaaaca cgaccaaaac atgcttggaa aatgctgata gcaaagttga
     1561 attgacttct gaattttgtg accactcgat gcgcatatcg ttaaaaaaca ctcttatata
     1621 aaacattaaa taaacgtagc aaatacctga acttaggcaa ggattattca agcaaaactt
     1681 gaccaaaagc gccaaaaaag agacacttac agttgatagc taggttgctg ggactcatca
     1741 ataatattca gcaacgtgat accataaaag cagtgtccaa tttcggaatc ccttcttggt
     1801 aatcaatact ttctcagcgc taggcatggc tcagtgcttg atgctaagtg ctagattata
     1861 cgcatagcga cttaaaactc ggagaatcct cacgcatggc aacgcattcg caagcacacc
     1921 tcaatgaaac tgacgcacaa gaaacgcaag aatggctaga agccctcagt gccgtcattg
     1981 agaatgaagg gccagaacgt gcccacttcc tgctggaagc aatgattgat caggcccgcc
     2041 gttccggcgg ccatttgcct tataaggcaa cgaccgctta cgttaatacc atccccacgc
     2101 atttgcaggc caaactgcaa ggcgaccccg agatggaacg ccgcattcgt gccctgattc
     2161 gctggaatgc aatcatgacc gtattacgtg ccaacgaaaa atcaccaggg attggtggcc
     2221 acatcgccag cttccaatct gctgcaacgt tgtacgatgt cggcttcaac cactttttcc
     2281 gcgcagccaa cgaaagcttt ggtggtgact gtatctactt ccagggccac tcctctccag
     2341 gcgtgtacgc gcgcgccttc ctcgaaggcc gcattactga agaacaaatg gacaacttcc
     2401 gccaggaaac gggcggcaat ggtttgtcca gctaccctca cccatggctg atgccggact
     2461 tctggcagtt tccaaccgta tccatgggtc tgggcccatt ggcagggatt taccaggcac
     2521 gcttcctgaa atacctgcac aaccgtggca ttgccgatac ggctgaccgc aaagtatggg
     2581 tattctgcgg tgacggcgag atggacgagc cggaatcaca aggtgcgata tcactggctg
     2641 cgcgtgaagg tttagataac ctgatttttg tcattaactg taacctgcag cgcctggatg
     2701 gcccggtacg cggcaatggc aagattatcc aggaactcga atccaacttc cgcggtgctg
     2761 gctggaacgc gatcaaggtt atctggggct cctattggga tccattgctg gccatggata
     2821 aagacggcct gctgcgcaaa cgcatggaag aatgtgtgga cggcgaatac cagaacttca
     2881 agcaaaaagg tggcgcctac acacgcgagc acttctttgg caaatatcct gagctgcgtg
     2941 aaatggtggc agccatgtct gatgcagaca tctggcgcct gaaccgtggc ggccacgatc
     3001 cacacaaggt gtttgcggca taccagtctg cagtgaatca caaaggtcaa ccaaccgtta
     3061 ttttggctaa gaccgttaaa ggctacggca tgggtgaagc gggtgaaggc cagaacacga
     3121 cgcaccagca aaaaagcatg gacattgcgt cactgaaggc tttccgtgac cgttttgacc
     3181 tgccactgac tgacgaacaa gttgagaacc tgtcttacta ccgcccggct gaagacagcc
     3241 cggaaatcaa gtacatgatg gcacgccgcg ctgcgctggg tggctttgtg ccggctcgcc
     3301 gccgtaaagg taacgagctg accgtaccgg agttgtccgc atttgaaaac atgctgggcg
     3361 cgacgggtga gcgtgaaatc tccaccacta tggcattcgt gcgtatcctg tctacgctgg
     3421 tacgtgacaa gcaagtcggt aaatatgtgg tgcctatcgt gccagacgaa gcccgtactt
     3481 tcggcatgga aggcatgttc cgtcaattgg gcatttactc atccgttggc cagttatatg
     3541 agcctcaaga ctctgaccag gtgatgttct acaaggaatc acaaagcggc cagatccttg
     3601 aagaaggcat taacgaagcc ggttcttttg ccagctggct ggcagctgcg acgtcctaca
     3661 gcgtcaccgg cacgcagatg attccgttct acatctatta ctccatgttc ggtttccagc
     3721 gtatcggtga ctttgcctgg gccgctggtg atagccgcgc acgtggtttc ctgttgggtg
     3781 ccaccgcagg ccgtactacc ttgaatggtg aaggcttgca acatgaagat ggtcatagcc
     3841 accttatgtc agccaccatt cctaactgta tttcctatga cccgaccttt gcttacgagc
     3901 tggctgtgat catccaggaa ggcatgcgcc gcatggtgca gaaccaggaa gatgtgtact
     3961 actacatcac cctgatgaac gaaaactata cccaccctgc gatgccagaa ggcagtgccg
     4021 aaggcatcct caaaggtatg tatagcttca gcaagtccaa ggccaaaggc cctaaagtac
     4081 aactgatggg ttcaggcgtg attctgcgtg aagtgattgc tgcggctgaa ttgctggaaa
     4141 aagactgggg cgtgtctgcc gacgtatgga gcgtgaccag ctttaccgaa ttgcgccgtg
     4201 atggtattga tgccgagcgc cataacttgc tgaacccgga agccaagcca aaactgagct
     4261 acgttgcaca agcgctggca aaagcggaag gcccagtgat tgcttctact gactatatgc
     4321 gttcgtttgc agaccagatt cacaactatg tgcctaatcg ttttgtcgca ctgggtactg
     4381 atggttatgg ccgttctgac tcacgtgaag cgctgcgcag cttctttgaa gtggaccgct
     4441 actacgtggt actggccgca ctcaaagcct tggcggatga cggcaagcta ccagcaagca
     4501 aagcgaccga agcgatcaaa aaatacaata tagacattac tcgtgccaac ccggccacga
     4561 tctaagtcac gacataagga aagtacaaaa catgtctatc aaagaagtac tggtgccgga
     4621 tatcggcaac tttgacagcg tcgatgtgat tgaagtcctg gtcagcgtgg gtgataccgt
     4681 tgccaaggaa gactcactga ttaccgtgga atcggacaaa gcctccatgg atattccctc
     4741 ttcactgtct ggtgtagtga aggaaatcca cattaaagtc ggtgacaaaa ccaaacaagg
     4801 ggcactgatt ctgaccctgg acaccgcagg tgaagcagcg ccagctgaaa aagtcgcacc
     4861 gcaaccaacg gcaacgcctg tcgttgccac ggtaacagta gatgaggcta aagtagcgat
     4921 tcctgagcct acacgcccgg ttgcagaagt accgcaggtg attcagccac aagcaacacc
     4981 caacccagtc gcagccagca cggttgccag cgatggaaaa ctggcacacg ccagcccttc
     5041 cgtacgcaaa tttgcgcgtg agttaggcgt gaacctgtca ctggtcaaag ccagcggccc
     5101 gaaaaaccgc atcctgcaat ctgacgtgca agcttacgtc aaaggtgaac tgtccaagcc
     5161 acgttctgaa aacatgggcg tgggtacagg cctgagcacc ttgccgatgc cggtcatcga
     5221 tttcagccag tttggtacta tcgaaaccaa accgttgtca cgtatcaaaa aactgtctgg
     5281 tgccaacctg caccgcaact gggtcacggc cccacacgtc acacagtttg acgaagcaga
     5341 cattaccgat ctggaagact tccgtaagtc tatgctggcc gaagcagaaa aacgcggtgt
     5401 gaaactgacc ttgctggctt tcctgatgaa agcggtggtc aacgcgctac gcacctaccc
     5461 caacttcaat gcctcgcttt cgccagaagg tgatagcctg attatgaagc agtactacaa
     5521 tatcggcttc gcctgcgata cgccagatgg cttggtcgtg cctgtggttc gtgatgtaaa
     5581 ccagaaagat gtgatggata tcgcccgtga cttggccgat ctgtctgcca aggcccgtga
     5641 gcgcaagtta aaggttgaag aaatgcaagg cggttgcttt actatttcca gcctgggtgg
     5701 gattggtggc acgatgttca cgccaattat caattgtcct gaagtggcca tcctgggtgt
     5761 ttcccgtgca gccatgcagc cagtctacaa taaagactcc ggcggctttg accctcgcct
     5821 gatcatgcct ctgtcattgt cctacgatca ccgtgtgatt gatggggctg atggtgctag
     5881 attcaccagt cacctgcgcg tgatgctgag cgatgtgaga cgtttactgc tctaagctag
     5941 tcacaacaag tcaaagggcg cttttagcgc cctttgtttt ttaaactagc tgattttgct
     6001 gcataaagtc acctgtgacg attatcgcct tattcaccaa tgaccattga tacaagtaaa
     6061 tccagactgg tttcaatatc acaccatcca taaccagcac ctgtgtttgc acacgctggt
     6121 attccgcagg ctgctcatcc agcgctccgc attcttcata atcatccagt atagccagcg
     6181 tacctcgcgc atcatgcaag cggtacacct ctccatacac acgatcacct ggattgtcag
     6241 aagcaatcac accgggataa taactgatct ggtacatctg cccttgaaac cagccgcctg
     6301 cgacaaacac ggcatggttg gcaagataag tagccatgtc attggtattg ccttgacgca
     6361 gggtgccata aacaaataac aaatcagaca tactggctaa aacaatacga gctgtggatg
     6421 actggatgat tgccgccagg catccggcac tttaaacaaa tccgtacgga aaccgagccg
     6481 ctctcccgac atacctaatt tctggcaact cagtttaaaa cgggtcgaca gcagatcagc
     6541 aaaaacgcct tcaccccgca tgcgttgccc gaaaccgctt tgatagcttt taccaccgcg
     6601 gctttgcctg acgatgctca tgacatgatt ggccttgagg gggaaagact gctgcagcca
     6661 ttgctcgaac aaatcgctta actcatgtgg cagacacaaa aagacataac ccgctttacg
     6721 ggcaccggcc tggtgtgctt tttccaaaat ctgttccata tcgcaatcgg tgagcgccgg
     6781 gatcacaggc gccaccatca ccccggtagg aatacccgcc tcgctgagtt tacgcaatgt
     6841 ttcaaacctg cgctcaggtg cagcagcccg aggctccagc cgacgtgcga cttgcctgtc
     6901 cagcgtcgta atgctgacat aaatactggc cagacgtttg gctgccatag gggcaatcag
     6961 gtcgatatca cgctcgacca gcgaagactt ggtgaccagg ctgaatggat gattggcctc
     7021 gcttaacacc tgaataattt gccgggtcaa ttgatattca cgttcaatcg gctggtaacc
     7081 atccgtattc atgcccagtg caatcggttt gcactgatat ttgggatgcg ccagctcttt
     7141 tttcaggagc gccacggcat ccgctttcat taacagccgc gattcaaaat ccagcccggg
     7201 agacatcccc agatacgcgt gcgtaggtcg tgcataacag taaatgcagc catgttcgca
     7261 cccgcggtaa ggattcaggg tgcggtcaaa aggcagatcc ggcgattgaa tatagttgat
     7321 gaccgatctg gagacatcca gcatgacttc cgtacgtaat gggggagcct cttcatcgag
     7381 gcctccccaa ccatcatcga gcgactcgcg ggtgtgtggg tcaaagcggc tgaccagatt
     7441 gctgctggcc gcacgccctt tgtaaataag aggtttttca ttcataaaac tcattcctga
     7501 aaaaaggcct ctgacaaaat gctattcgtt gttgttttta ccctgccaaa accttgagtt
     7561 gcatatagta agtgccatca tcagcgtttt tctgaaatcc actggcaagc cagcgcgctc
     7621 cagtaaggca gtgactgtaa agccagcatg gagacccata actgcgcatc cagattgcta
     7681 aagccgcgcg agtagatcat ggccacggca caaagaagca gggaaatcag aatcgtcagc
     7741 tcctctttga tgggcgcgat gagttgccag ctgctgagtt ttgccttggt tttattcgtg
     7801 acgacaaaga caccggcctt gtgtgtcaag ccactgataa tccccctggc aattgcatgc
     7861 gataagccca ggctagctag cgacgcgcca aagatatcca tccagctaca atgcatggtc
     7921 ttgcggtata gcactggtcc caagatggct ttgatcgcca ggaaacacag aataggcaca
     7981 atcatgacca ccactggcaa actgaaatac tttgggaatg ccagcatggc aattgtccag
     8041 ccaatagagc caatggtgaa tagcagttgc aaggcctcgc caaaccagcc aaaccagcca
     8101 gttaaaaagt ggtagcgctg gctgaagttg agcgatgact tgcccaatag ttttggcata
     8161 tggtgtttga gaatttgcat ggcgccaaac gcccagcggt tacgctgcga cttaagcgcc
     8221 ttgaagttgg ctggcgtcag gccacggcca aaagtctcat caatgtagcg caactccata
     8281 ttgttctcaa gtaaacgcag gcctagttcg gtatcctcac aaatacacca ctctgaccac
     8341 ccaccaaagc cttttaaaga ctctgcacga ataagagtca tggtgccgtg ctggatcagg
     8401 gcattacgct catggcggtg gtgcatcccg attcggaaaa agccttcaaa ctcccagtta
     8461 cacatgcggt ggaagaagct atgttcccat tcacggtgtg cctgcggcgc ctgcaccacg
     8521 gccacctggg attccaggaa gtgcggcacg aggtcagaca accagtctgg tgtgactaca
     8581 taatcagcat ccactacccc cacgacttgc gcatcaggat gtgttttatc cagggcaaaa
     8641 ttcagcgctc cggctttaaa gccaggccag gaaggtaaat gatagaaatg gaagttatcc
     8701 ggcatattgg ccatgtaagc ctctagcggt ttccatttgg cttcatcctt ggtgttgtta
     8761 tccaccacga tcacttcata gcgggtgtag ttcaagttac gtaaactgtc gatggtggca
     8821 atcaccattt ccggcggctc gttatagcaa gccaggtgga tagaaacaaa cagttcctgg
     8881 tctgcaggca atggctgcgc gcgcttgaat agacgtcgcc agtaaggctt gaacatgacc
     8941 tcgctaaact ctgccgcata aatcatcaac accatggagg tgagtgcaat gcctaaaatc
     9001 agcccgacca gtacaacaaa gtcgcgtagc gtgtagtagt aatctcctgg caggttccag
     9061 gcgatggtga gcgtggtaat acaggcctgg aacaagaccg ccatcgacaa tacgccacct
     9121 atgccccaat gcttgaagcg ccaggcgatc aacatcagcg gaataaaagc caatgcggtg
     9181 gaccaggtcg ccttggcaat ccagcgggta ttaggcggca cagcgccctg taaggaatac
     9241 ttctggtgac ggtcagcgtt atacatcccc caatacgccc cggcccaccc ttccagtgcg
     9301 accttccagg gctggtcaaa cgcttccatc aggtaaaaat ccaggtcctc aatagagtta
     9361 cgtgccaaaa actcccggat aaaccgcgcc tggttgactt cgctggccac agaagcgcca
     9421 atggctggac cgcggctagg ccagccgact tcggtaatca cgattttctt gcgcggataa
     9481 gcttccatca actggttgta gcgctcaatc gcatattgca ccgccttttc gacgggcacg
     9541 ccttcatggt atggcaacag gtggaccgca atatagtcta cgtgtttaac gagttccgga
     9601 tgcttaatcc agacatgcca tggctcggca gtggataccg gcacattgct atgctcacgt
     9661 acaaaatcca ggtaaccgat taatgcctgt actggcaagt cattacgtaa caaggcctcg
     9721 ttaccgacaa tcacacggtc aatattgctg tagttttcca gcttttccag cagcgccgac
     9781 acttcacgcg tatttttcat gttgtcggtg tcaatccagg cgccggcaat tacgttcata
     9841 ttgcgtcttg ccgcgagtgg gatcacctca ttctgatcca atgaggagta catacggata
     9901 tgacgcgtat gcttatgcat caagtccaga tcttctgcca actctgctgt actcggatgc
     9961 tgcccttcga gcgggctttg attggcacga tagccggtat acgcaaaccc gttcacggta
    10021 ggcttgacat tgatgagcgg caaggcacga ttggttaatg cccacagccc aaaatgcaag
    10081 gctgaaaaca ggatggcaaa taccaggcca ggaatcagtt ttttaaacca ggaaacgatc
    10141 acttctcact ctttccatca tttgatgatg tgtttattta aatcttgtat taacggctaa
    10201 gttgcaggct tctggcaggc agcacaaaca gggccaagcc acatgctcca caccaccacc
    10261 atgccatgat attgccaggc tccatcatgg ccacggccac cgccatattg gaggccatga
    10321 cgcccatgac cgcactcaac caaaaaccgg cttgattgaa ggccgcagca gattgctcaa
    10381 ttttggtcga agaaatcagc gaaatggtga tcaacaccaa tggcaggcaa accaggatca
    10441 gtgggaaatc gagatagcgg ccattaatcg ccaggcccgc actggtcaat aagaacgcca
    10501 gcgcaagcaa tcgtaccaag gactgttcga tacagctgta cacctttaat cccagccact
    10561 gccgtagctg tagccacgcc aacgcccagc ctagcacgac cagcccaccc agcatgacat
    10621 attcgccagt attgcgcgca gccatatgaa cataatccac ttgcaagtat gcgtgcaagc
    10681 ctgcggccag cccagtcaag gcaagcaata ctttatagcg ggctgacagt tgtgtgtact
    10741 gcccccacgc cgcgccaagc actgccagca ctagtgcaac cagataaggt ttaaagccat
    10801 cagaacgttc ggccatgctg ccttgcaaac tgaatttggg ttgcaagcct gtatccagca
    10861 ccccccagta gccacctacc gtaccctcca gctcgcgctt ccatggctgg tcaactgctt
    10921 caatcacatt gtattgccaa tggttttgct gggcggcatt taaaaaaccg cgcatgtaga
    10981 gcgcctggtt gatggcactg ggtttagacc agaagcgctg gcgcccttgt gatggccaac
    11041 cggtttcgcc aatcaggatt ggctttttaa acacggtgcc cagatgcccc atgacggaag
    11101 ccgtatgcgc aaccgcctgc tcaatcgcaa ccggtttgtc ttcccagtaa ggcaggatat
    11161 gcacagtgac gaaatctaca tcctgttcca atgtcggatg cttgagccaa aactcccaga
    11221 catccgcata ggtaatgggt gctttggtat tggctttggc ccattgcagg tactcatgca
    11281 tcttgggctc ggactgctcg ccacgcaaca acacctcatt gccgaccacc aggccacgca
    11341 cagtatcagg gtattggttt gcctgctgca cagcaagttg caattccttc ctgttcaggt
    11401 catcaaccca gccgatccag actcccagaa tcaccgtcat gcccaacttt tgcgcggcct
    11461 tgggaacata atccaggcct tgtgaaactg aatacgtgcg tacgcacttt gtgactttgg
    11521 ataatgcctg caagtcctgc tcgatctgca ccgggtcgac aaaactgtct ttcactgtgg
    11581 ggttcatgcc cggcttgtaa tacggggcat acgaaacaca ttgcaatggc aatgcgtccg
    11641 tagcggcatc aatcaatact tgtggctgtt gcagacgcca tacatagtag agagagccga
    11701 tcagaaacaa ggtcagccag accactggtg aaactcgaga taacaaacgc tgcaatatga
    11761 tcaccctgaa cggaattgct aaaaaatagc ggttattctc tcataaatag cactgccatg
    11821 cgtcttacac atatagatga catggcacaa atattccaaa a
//
LOCUS       DATMSK010000159         5555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_388119,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000159 DATMSK010000000
VERSION     DATMSK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388119"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..627
                     /locus_tag="VNZ84_05345"
     CDS             130..627
                     /locus_tag="VNZ84_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HWU66414.1"
                     /translation="MLKKLLIAASLLVSTSAFATSTTITFSGTDLSGSSDARTAKISI
                     SSAVDFDLTGILDPSFRFLQSNNSGLYQGLTLISDANSTYTYAPVILPLSDFDLTRYT
                     FSVSNLAAGNYTLQFNLIGGGNYAGSYTINPITTAVPEPETYGMMFAGLALMGVIAFR
                     RQKNS"
     gene            complement(699..1436)
                     /gene="kdsB"
                     /locus_tag="VNZ84_05350"
     CDS             complement(699..1436)
                     /gene="kdsB"
                     /locus_tag="VNZ84_05350"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HWU66415.1"
                     /translation="MFKVVIPARFASTRLPGKPLLDIAGKPMVIRVAEQAAQSQASEV
                     VIATDHEAVMQAAQAHDIATVMTRADHLSGTDRIAEVVKIMHWPDEMIVVNVQGDEPL
                     IDPVLINEVAQTLANDPQAVMSTACHPLHTPEAFENPNVVKVVLNAKQQALYFSRAAI
                     PFPRDAEYRQHIVTHRHIGIYAYRVGFLKYYASLPVTELEKIESLEQLRALYHGYQIA
                     VTVTAHAPASGVDTAEDLDMVRKVFAG"
     gene            complement(1439..1621)
                     /locus_tag="VNZ84_05355"
     CDS             complement(1439..1621)
                     /locus_tag="VNZ84_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HWU66416.1"
                     /translation="MNPKLLEILVCPVTKGPLVYDKQKQELVSKAARLAYPVRDGIPV
                     MLEDEARRLDEHEYSE"
     gene            complement(1625..2656)
                     /gene="lpxK"
                     /locus_tag="VNZ84_05360"
     CDS             complement(1625..2656)
                     /gene="lpxK"
                     /locus_tag="VNZ84_05360"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWU66417.1"
                     /translation="MLRQWFERQWQMTGWAQCLLLPLSWLFALLATARRWCYKAGLFS
                     SQDLPVPVIVVGNLSVGGVGKTPMVIYLAQQLKAAGYTPGILSRGYGGQHTGEVTSNS
                     PPELCGDEPVLIARRSGCPVWVDASRVVGGLALIKAHPQVNVVICDDGLQHYALQRDI
                     EIAVVQRPLGLGNGRLLPAGPLREPLGRLQQVDIVIESGQLPVALALARTYQLKLRSG
                     TWTAVNDFESEISTEALREQSLVAMAGIGHPQRFFEVLETMGLQCEQRALADHYAYTQ
                     TDFESLHGKTVLMTEKDAVKCQYLGLSRAWFLPVSAELEAVGHKQSLIVDITERLDAI
                     RATKQRQRK"
     gene            complement(2656..4410)
                     /gene="msbA"
                     /locus_tag="VNZ84_05365"
     CDS             complement(2656..4410)
                     /gene="msbA"
                     /locus_tag="VNZ84_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HWU66418.1"
                     /translation="MARKSSSESVPISNPVLLYKRLFAYAWHYKFQFALSMVSTAILA
                     SSNTFFLALIKKVTDEGFTRSAGQAAYVLPLMLFGLMAIRAVAGFFSVYSLRTVARQV
                     VEVMRKQIFAKLLMLPVSFFDANSNGLLVSKMTYDIERLSTVVTRSALNVLRDVLTVI
                     GLIGYMLYLDWRLTLIFAMVAPLMGLYLARTTPKLRANAKKVQQAVGEITKTAEEAIA
                     AQRIVKIFGAQAFENDRFGAVVSRNRQLELRTARISGLNSFVIEILSALVLGLVVFYA
                     LSQFTVGEFAAFVGALMMLIAPIKHITSANEDLQIGLAAAQSVFDVLDIENEVDQGSY
                     QIARVQGKLKFDQVTVRYPQAKKDALSALSFEIHAGEKVALVGRSGSGKTTLVNLLPR
                     FYESQQGSVLLDDHDVREYQLDNLRAQFSLVSQDIVLFNDTIFNNIAYGALRDASEQE
                     VIEAAKAANAWEFIQQMPEGLQSEIGDRGVRLSGGQRQRIAIARAILKNAPVLLLDEA
                     TSALDSESELHVQMALDRLMRDRTTIVIAHRLSTVENADRIFVMDHGKIIETGSHQTL
                     LAENGYYSRLYHKQFQDD"
     gene            complement(4500..4928)
                     /locus_tag="VNZ84_05370"
     CDS             complement(4500..4928)
                     /locus_tag="VNZ84_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWU66419.1"
                     /translation="MNFKRGVRDEELEINFIPLIDVLLVIIIFLIVSATFSRTSELQI
                     NLPTADANAPQEKPLNITVEVDASGRYLVNGKEANGAVVSDLSAALTQAGAEGPSGKE
                     PTIIINADAKATHQSVVNIMEAARMANYTHITFATQVNSH"
     gene            complement(4965..>5555)
                     /locus_tag="VNZ84_05375"
     CDS             complement(4965..>5555)
                     /locus_tag="VNZ84_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWU66420.1"
                     /translation="LAAGWPIWPLIIASVISLAIIVERFWALRASVVTPDQLLPEVQN
                     WLKQGGVTRETLSRLEAHSLLGKVFASALANMNNSREVTKEAIEETGRAVAHKLEQYL
                     PTLGTIATVAPLLGLLGTVIGMVELFGSFTNSGHDVAQFARGISVALYNTAAGIVVAV
                     PSMIAYRYFRSKVDGFLVDMEQQAIKLVEIIHGDRG"
BASE COUNT         1473 a         1494 c         1241 g         1347 t
ORIGIN      
        1 catgatggga gctgtttatt ttatcttcag aacctgagcg actagcccta atgggctatt
       61 tctacaatgt cacaaaacag ccataatccg tttcaagtaa tactttaatc atatctctcg
      121 gggaaaatta tgttgaaaaa actcttaatc gcagcttcat tgctggtaag tacgtctgct
      181 tttgcaacaa gtacaacaat cacgttcagt ggtaccgact tatctggctc tagcgacgca
      241 agaacagcta aaatcagtat tagctcagct gtggatttcg atttaacagg gattctcgat
      301 ccaagcttca gatttctgca aagcaacaat tctggtttgt atcaaggcct gactctgatc
      361 agcgacgcaa acagcacata tacgtacgca ccagtgatct tgccactcag tgattttgat
      421 ctgaccagat atacatttag cgtgtctaat ttggctgcag gtaattacac attacaattt
      481 aacctgattg gtggtggcaa ctacgcaggc agttatacca ttaacccaat cactacagcg
      541 gtaccagaac cagagactta cggcatgatg tttgctggct tagccttgat gggtgtgatt
      601 gcattccgcc gtcagaaaaa cagctaatca gcgatctatt aagctgcaat aaaaaagcct
      661 cacattgttg aggctttttt attttgacga tgctgaagtt aaccagcaaa cactttccgc
      721 accatgtcta aatcttccgc agtatcaacc ccactggcag gcgcatgtgc cgtcacggtg
      781 actgcaattt gataaccgtg atataacgcg cgcaactgct ccaggctctc aatcttttct
      841 aactcagtga ctggcaaact ggcgtaatac tttaaaaagc ctacacggta agcataaata
      901 ccaatgtgcc ggtgggtgac gatatgctgg cgatattcag catcccgtgg gaaaggaatc
      961 gccgcacgac tgaaatacaa ggcttgctgt ttggcattca aaacaacctt gaccacattt
     1021 ggattctcaa atgcttcagg cgtatgcagt ggatgacacg cagtcgacat gaccgcttgc
     1081 gggtcatttg ccaaggtttg tgcgacttca ttaatcaata ctggatcaat cagcggctca
     1141 tcgccctgca cgttcaccac aatcatctcg tccggccaat gcataatttt aaccacttcg
     1201 gcaatgcgat ctgtgccgga aaggtggtca gcacgcgtca taacggtagc aatatcgtgt
     1261 gcctgtgctg cctgcatgac agcttcatgg tcagtcgcaa tcacgacttc actcgcctgg
     1321 ctttgtgctg cctgctcagc cacgcgaatc accatgggtt taccagcaat atccagcaaa
     1381 ggcttgcctg gcaaacgtgt gctcgcaaaa cgggcaggaa tgacaacctt aaacatgttt
     1441 attcgctata ttcatgctca tccaggcggc gcgcttcatc ttccagcatc accgggatac
     1501 catcgcgtac tggataagcc aggcgtgcag cttttgagac cagctcttgc ttttgcttat
     1561 cgtacaccag cggtcctttg gtcactgggc ataccagaat ttccaatagt ttaggattca
     1621 ttttctactt cctttgtctt tgctttgtgg cacggattgc atcaaggcgc tcggtgatgt
     1681 caacgatcag tgattgcttg tgacccacag cctcaagctc ggcgctgacg ggtaaaaacc
     1741 aagctcgcga taatccaaga tactgacatt ttacggcatc tttctccgtc atcagtacgg
     1801 ttttaccatg cagcgactca aaatccgttt gcgtataagc ataatgatca gccagtgcgc
     1861 gttgctcaca ttgcaatccc atagtttcca acacttcaaa aaaacgctgt ggatgcccaa
     1921 tccctgccat ggccaccagg gattgctcac gtaatgcttc agtagagatt tcactctcaa
     1981 aatcattcac agccgtccag gtgccgctac gtaatttaag ttgataggtc ctggcaagcg
     2041 caagcgctac aggtaactgg ccactttcta taacgatatc cacctgctgt aaccggccaa
     2101 gtggctcacg taatggtccg gcaggtaaca atctgccatt ccccaatcct aatggccgct
     2161 gtactacagc gatttcgata tcacgctgca aggcataatg ttgcaggcca tcatcgcaga
     2221 ttaccacatt gacctgggga tgggctttga tcaaggctaa cccgccgact acacgagaag
     2281 cgtccaccca cacagggcaa ccgctgcgtc tggcaatcaa taccggttca tcgccacaca
     2341 gctctggcgg actattagac gtgacttcac ctgtgtgctg acctccatag cccctgctca
     2401 aaataccggg agtgtagcct gcggctttta attgctgtgc caggtaaatg accatcggcg
     2461 ttttacccac gccgcctacg gaaagattac ccaccacgat cacaggcaca ggcaagtcct
     2521 ggctggagaa aagacctgcc ttgtaacacc agcgtctcgc tgttgccaac aaggcaaaca
     2581 accaggataa cggtaacaac aggcattgtg cccagcctgt catttgccac tgtcgttcaa
     2641 accactgccg caacatcagt catcttgaaa ctgtttatgg tacagccggc tgtaatatcc
     2701 attctcggcc agcaaagtct ggtgtgaacc ggtttctatg atcttgccat gatccatgac
     2761 aaaaatcctg tcggcatttt caaccgtact caaccggtgt gcaatcacaa tcgtcgtgcg
     2821 gtcacgcatc aggcggtcca gtgccatctg cacatgcaat tctgattcgc tatccagtgc
     2881 agaagttgcc tcgtcaagca gcaacacagg tgcatttttt aaaatggcac gtgcaatcgc
     2941 gatacgctgg cgctgaccgc cggacaagcg cacaccgcgg tcgccaattt cactttgcag
     3001 gccttctggc atctgctgga taaactccca ggcattggcc gccttggcgg cctcaatcac
     3061 ttcctgttca ctggcgtcac gtaacgcacc ataggcaata ttgttgaaaa tcgtatcatt
     3121 gaacaatacg atatcctggc tgaccaatga gaactgcgcg cgcaggttat ccaactggta
     3181 ctcccggaca tcgtgatcat ccaggaggac gctgccctgc tgcgactcat aaaaacgcgg
     3241 caacaggttc accagtgtgg ttttaccact gccagaacgg ccgacgaggg caactttttc
     3301 gcccgcgtgt atctcaaaac tcagggcaga caaagcatct ttttttgctt ggggataacg
     3361 cacggtgact tggtcaaact ttaactttcc ttgtacacgt gcaatttgat aactgccctg
     3421 atccacctca ttctcaatat ccagcacatc aaacacactc tgcgcagcag ccagcccgat
     3481 ctgcaaatct tcgttggctg aggtaatgtg cttaattggc gcaatcagca tcatcaacgc
     3541 gccgacaaag gcggcaaact ctcccacagt gaattgtgac agcgcataaa acaccaccag
     3601 ccccaatacc agtgcagaca aaatttcaat cacaaagctg ttcaaaccgg agattcgtgc
     3661 agtccgtaac tctaattggc ggttacggct cacaacggcc ccaaaacggt cgttctcaaa
     3721 agcctgcgca ccaaaaatct tgacgatgcg ttgcgcagca atcgcctctt ccgccgtttt
     3781 ggtgatctcc cctactgctt gctgcacttt ttttgcgttg gcgcgcagtt tgggggtggt
     3841 gcgggcaaga tacaaaccca tcagcggagc aaccatggca aaaatcagtg ttaaacgcca
     3901 atccaggtaa agcatataac caataagacc aatcaccgtc agcacatcac gcagtacgtt
     3961 taatgcggag cgggtgacta cggtagagag gcgctcaatg tcgtaggtca ttttagaaac
     4021 cagcagccca ttagagttgg catcaaaaaa actcaccggc aacatcaata atttggcaaa
     4081 tatctgctta cgcatcacct cgaccacctg ccgggcgact gtacgcaggc tatacacaga
     4141 gaaaaaccct gccaccgcac ggatcgccat cagaccaaat agcatcaatg gcaacacata
     4201 agctgcttgc ccggcagacc gggtaaaacc ttcatcagtc acctttttaa tcagggccag
     4261 gaagaaagta ttgctggacg ccaatatggc ggtagaaacc atggataagg caaattgaaa
     4321 cttatagtgc caagcatagg caaataaccg tttatataat aaaacgggat tgctgatagg
     4381 gactgattcg gaggaagact tacgtgccat ggctttttct caggcagact aaaaacaaac
     4441 agcccgccaa ggttgacggg cttgttgatg ggcgactgtg atatgtagcc cgtgatacat
     4501 taatgcgaat ttacctgcgt cgcaaaagtg atatgggtgt aattggccat acgcgcagct
     4561 tccatgatat tcacgaccga ctggtgtgtc gccttggcat cggcattaat aataatcgtg
     4621 ggctctttgc ctgacgggcc ttctgcacct gcttgcgtca gcgccgccga caggtcagat
     4681 accacagcac cattcgcttc tttgccatta acaaggtaac ggccactcgc atccacttca
     4741 actgtaatat tcaatggctt ttcctgtggt gcgtttgcat ccgccgtggg caggttgatc
     4801 tgcaattcgc tggtgcgtga aaaagtggca ctgacaataa ggaaaatgat aatgaccagc
     4861 aacacgtcga tcaacggaat aaagttgatt tccagctctt cgtcacggac accacgtttg
     4921 aaattcatga gattgccttt gtttccaatg tcctgttaag cgggttatcc gcgatcaccg
     4981 tgaataattt caaccaactt gatcgcctgc tgctccatat ccaccaggaa accatcgacc
     5041 ttgctgcgga aatagcggta agcaatcatg gaaggaactg ccaccacgat accagccgca
     5101 gtgttgtaca acgcgacgga aataccacga gcaaattgcg ccacgtcatg cccactgttc
     5161 gtaaaagaac caaacagttc aaccatacca atcaccgtcc ccaataagcc caataaagga
     5221 gccaccgtgg caatcgtgcc caaggttggc agatattgct ccagcttgtg agccacggca
     5281 cggccggttt cctcaatggc ctccttggtc acttctcgtg agttattcat attagccaaa
     5341 gcactggcaa acaccttacc cagtaaagag tgtgcttcga ggcggctcag ggtctcgcgt
     5401 gtcaccccac cctgctttaa ccagttttgt acttctggga gcaattgatc aggcgtcact
     5461 accgaggctc ttaatgccca gaaccgttca acaataatcg ccaaagaaat cacggaggca
     5521 ataatcagag gccagatagg ccagccggcc gctaa
//
LOCUS       DATMSK010000160         5595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_11806,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000160 DATMSK010000000
VERSION     DATMSK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11806"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /gene="ccoG"
                     /locus_tag="VNZ84_05380"
     CDS             <1..1212
                     /gene="ccoG"
                     /locus_tag="VNZ84_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HWU66421.1"
                     /translation="IFGLIFHPQDLIYLSFLLIVSALALFLFTAVAGRLWCGYTCPQT
                     VYTEIYMWMEQKIEGDRIARMRLDDNKWNLNKITRKSLKQFAWIGFGLWTGVTFVGYF
                     SPIRELVPAIFEWQLGGWETFWICFYGFATYGNAGYMREQVCKYMCPYARFQSAMFDG
                     DTMTVTYDEARGEPRGARSKKAGENANGLGSCIDCSICVQVCPTGIDIRDGLQYECIG
                     CGACIDACDDVMDKMGYAKGLIKYTTQNGLENRWSRQQIWRRLLRPRVLVYTAILSAL
                     TIGMVVSLYFKPDFRLDVLRDRAMMARYTNEGKIENVYNLKLENDTESTRDYTLQVTG
                     LAEIAVHISDDNGQPFKALKPFETRNVIVELQIPDGVTNAGSHKIYFEMTDVESKDTV
                     KEKSVFIVPSN"
     gene            1243..1545
                     /locus_tag="VNZ84_05385"
     CDS             1243..1545
                     /locus_tag="VNZ84_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HWU66422.1"
                     /translation="MSNTQAMPNHGTSLNADQKWYRNPFVWLIIGGPLLVVVASFITL
                     YLAIKHPDPAIDDYYRKGIEINKTLDAQGYDTQSESMAPAVQARNHAQTGLRPAEE"
     gene            complement(1564..2412)
                     /locus_tag="VNZ84_05390"
     CDS             complement(1564..2412)
                     /locus_tag="VNZ84_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66423.1"
                     /translation="MEPSPLFTALTAYAATCGWQYIYPVWSRRADGLSIGINLHPNHC
                     CNWHCIYCQIPGLQRGPSPLIDSRRLQVELTDCLDWLSHHLSPTLTMQDSVQDIAFAG
                     DGEPTTSPQFVEVLLMVANLLQARQSHDRPATVRLITNGSQCQHAHVQHALKLLSGMG
                     GEVWFKLDAGNDAEMQAINASHLPLGLHMQRLHTCCSICPTWVQTAIVSRTLGEKTVT
                     TPSLPEYLQVLVPLKAHIQGVLLYGIARPSQQDTQGCILRTTEAMLRRYEEGLRTQGF
                     RVRMFA"
     gene            complement(2444..3163)
                     /gene="fnr"
                     /locus_tag="VNZ84_05395"
     CDS             complement(2444..3163)
                     /gene="fnr"
                     /locus_tag="VNZ84_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HWU66424.1"
                     /translation="MQAESSFKVACSNCNLRELCLPIGLSTDEMKQLDGMVATRKKVK
                     QGGHLYSSGDPFTTLYAIRTGFFKTCVVSEDGREQVTGFQMAGEIMGLDGIVSDKHNC
                     HAIALEDAEVCVMPFNHVEDLSREIPMLQRHVHKIMSREIVRENGVMMLLANMRAEER
                     LAAFLLNLLQRLHARGYSKSELILRMTREEIGSYLGMKLETVSRTFSKFSEDGIIEVK
                     QKNIRILDHEKLKTIFNSSTC"
     gene            3403..4788
                     /gene="hemN"
                     /locus_tag="VNZ84_05400"
     CDS             3403..4788
                     /gene="hemN"
                     /locus_tag="VNZ84_05400"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HWU66425.1"
                     /translation="MKQPLMITQEHIERYDVSGPRYTSYPTADRFVEAFDAAAYYKSA
                     EQRNISNWTQPLSVYVHIPFCASLCFYCACNKIVTKRYEQSAIYLNYLQRELGLHLNK
                     IGKAQAVNQLHLGGGTPTFLNDEELSSLMQMLKTAFVFSEEGEYSIEIDPRTVNQVRL
                     QHLRDLGFNRLSFGVQDFDAEVQQAVHRVQSYTQVETLMQQARQMGYQSINADLIYGL
                     PKQTPATFRETISKITQLRPDRIALYGYAHLPSRFKPQRRILQEDLPNAVDKIQMLSE
                     AIQQLMQAGYVYIGMDHFALPTDALAIAKQQGRLHRNFQGYSTMADQDILALGVSAIG
                     KMGATYSQNAKTLEEYYDFLDQGQLPIVRGLALSRDDLIRRAVIMAIMCHGELSYESI
                     DLSYMVNFRDYFKSEMQQLVEKEKQGLVILEDDGLKVTEMGWYFVRAIAMVFDRYLQH
                     DANRARFSKIL"
     gene            4798..5514
                     /locus_tag="VNZ84_05405"
     CDS             4798..5514
                     /locus_tag="VNZ84_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWU66426.1"
                     /translation="MLASLLLTAFVMGLAGGPHCIAMCGAACGAMQSSSQTTSQFRSI
                     PLRVVKTIPWSFYLGRMLGYGLLGALAAETLRGLAWLSNQSHVFQPLWTFSHTLIFAW
                     GVLLLVTGKQPQWAIKRAQQVWVWIKQRTQHDRVGFLGTGMVWALLPCGLLYSAVMLA
                     SLQSQWLQGALVMMAFAAGSATVLAIAPLVWRSGQGALPNWMRESTGMRLSGIFLISL
                     SLIALWMDLMHHDKLWCQVL"
BASE COUNT         1339 a         1379 c         1449 g         1428 t
ORIGIN      
        1 atttttggcc tgatattcca tccgcaggac ctgatttatc tgtcgttcct gctaatcgtt
       61 tcagccttgg cgctgttcct gttcacagca gtggcggggc gcttatggtg tggctacacc
      121 tgtccgcaaa cggtgtatac cgaaatctat atgtggatgg aacagaagat agaaggtgac
      181 cgtattgctc ggatgcgcct agatgacaac aagtggaatc tcaacaaaat cacccgtaag
      241 tcgctcaagc agtttgcctg gataggattt ggcctgtgga ccggtgtgac ctttgtcgga
      301 tatttctctc ccattcgcga actggtcccc gccatttttg aatggcaact aggcggttgg
      361 gaaaccttct ggatctgctt ctatggcttt gcgacttatg gtaacgcagg ctatatgcgt
      421 gagcaggtct gtaaatacat gtgcccctat gcacgtttcc aaagtgccat gtttgacggc
      481 gataccatga cggtgaccta tgacgaggca cgtggtgagc cgcgtggcgc acgctccaaa
      541 aaagcgggcg aaaatgccaa tggcttaggc agttgtattg actgtagcat ctgtgtgcag
      601 gtatgcccga ctggcattga tatccgtgat ggtctgcaat atgagtgtat cggctgcggt
      661 gcctgtattg atgcctgtga cgacgtcatg gataaaatgg gttatgccaa gggcttgatc
      721 aaatatacga cgcaaaatgg cctggaaaac cgctggagcc gccagcaaat atggcgtcgc
      781 ctgctgcggc cacgtgtgct agtttacacc gctattctat cggctttaac cataggcatg
      841 gttgtcagcc tgtatttcaa acctgacttc aggttggatg tgttgcgtga ccgtgccatg
      901 atggcgcgct ataccaatga aggtaagatt gaaaatgtgt ataacctcaa actggaaaac
      961 gatacagagt ccacgcgaga ctataccctg caagtcactg gattggccga gatcgccgtc
     1021 catatctcag atgataatgg ccagccattc aaagcactca aaccttttga aacccgcaat
     1081 gtgatcgtgg agttgcaaat cccggatggc gtgaccaacg ccggttcgca caagatttat
     1141 tttgaaatga cggatgtcga atccaaagat acggtaaaag agaagtcagt gtttatcgtg
     1201 cccagtaatt aatgcaccag gaatttaacg gagtcacaaa ccatgagtaa tacgcaagca
     1261 atgcctaatc acggaacatc tttgaacgct gaccaaaaat ggtaccgcaa tccgtttgta
     1321 tggctgatca tcggcgggcc tttgcttgtg gttgtggctt cttttatcac cctgtacctg
     1381 gccatcaaac atccggatcc ggccatcgat gattattacc gcaagggcat tgaaatcaat
     1441 aaaaccctgg atgcccaggg ctatgatacg caaagtgagt ccatggcacc tgcggttcag
     1501 gcacgtaacc atgcccaaac gggtttaaga ccggccgaag aataagctta cacactttac
     1561 gtgtcaggca aacatacgca cgcggaatcc ttgcgtgcgt aacccctctt cataacgcct
     1621 caacatagct tccgttgtgc gcaggatgca gccttgcgtg tcctgctggc tgggtcgtgc
     1681 aatcccgtag agcaaaacac cttgaatatg cgcttttaga gggactaata cctgcaggta
     1741 ttccggcaat gatggcgtag tcaccgtttt ttcacccagt gtcctgctca cgatggctgt
     1801 ctgtacccag gttggacaaa tggagcaaca ggtgtgcaag cgctgcatat gtaatcccag
     1861 cggcaagtgg gaggcattga tggcctgcat ctcggcatca ttgccggcat ccagtttaaa
     1921 ccatacttct ccacccatcc cgcttaatag cttgagtgca tgttgaacgt gcgcgtgttg
     1981 gcactggctg ccattggtaa tcagccttac tgtcgcaggg cggtcatgcg actggcgtgc
     2041 ctgcaaaaga ttggccacca tcaacagcac ttctacaaac tgcggcgatg tcgttggttc
     2101 gccatcacct gcaaaagcaa tgtcttgcac actgtcttgc atggtgaggg ttgggctcaa
     2161 gtggtggctc aaccaatcca ggcaatctgt gagttctacc tgcaagcggc ggctgtcgat
     2221 gagtggtgaa ggaccgcgct gcaagccagg gatctggcag taaatgcaat gccagttaca
     2281 gcagtgattc gggtgcaggt tgatgccgat agagaggccg tcggcgcggc gggaccacac
     2341 aggatagata tattgccagc cacaggtggc tgcataggcg gtcaaggccg taaatagggg
     2401 agagggctcc atgcactgaa accggtggcc agcaaaatcc gtgttagcag gtgctggaat
     2461 taaaaatggt tttcagcttt tcgtggtcga ggatgcggat atttttctgc ttgacctcaa
     2521 taatgccgtc ttcactgaac ttggagaacg tacggctgac ggtttccagt ttcataccca
     2581 gatagctacc gatttcctca cgtgtcatgc gcaaaatcaa ttcgctctta gagtagccac
     2641 gggcatgcaa gcgttgtaat aaattcagca ggaaagctgc caggcgttct tccgcgcgca
     2701 tattggccag taacatcatc acgccatttt cacgcacaat ttcacggctc atgattttgt
     2761 gtacatggcg ctgcagcatg gggatttcac gggacaggtc ttcgacatgg ttaaacggca
     2821 tcacacagac ctcagcgtcc tcaagtgcaa tcgcgtggca gttgtgtttg tcgctgacaa
     2881 tcccgtccag gcccatgatt tcaccagcca tctgaaaacc cgtgacttgc tcacggccat
     2941 cttccgacac gacacaggtt ttaaagaaac cggtgcggat ggcatacaag gttgtaaatg
     3001 ggtcgccgct actatacagg tggccgcctt gtttcacttt cttgcgggtg gcaaccatcc
     3061 catccagctg cttcatttca tcggtgctta aaccgatggg gaggcacagc tcacgcaggt
     3121 tgcaattaga acaagcgacc ttgaagctgg attcggcttg catgggaggt gtccttgtct
     3181 gtcgttgaac atgcgaagga ttatacgcct cttcagagat ggtttcattg aacatggtga
     3241 tctatcttcc cttttatatt gcgttgcatt atgcatggga cggcctagac agcgtttgat
     3301 ttgcatcaag aagtcatcta ttgctttgtg gcaaagtggc atccaatgat cccggggctg
     3361 ctcgccccca ctgtgctgac aggaaagaat ttgctagaaa ccatgaaaca accgttgatg
     3421 attacccagg agcatatcga acgctatgat gtttccgggc cgcgttatac ctcttatccg
     3481 acggcagacc gctttgttga agcttttgat gcggctgcct attacaagtc ggcagagcag
     3541 cgcaacatca gtaactggac gcagccactg tcggtgtatg tgcatattcc gttttgtgcc
     3601 tcactatgtt tttattgcgc ctgcaataaa attgtcacca aacgctatga gcaaagcgcc
     3661 atttacctca attacctgca acgtgaactt ggcttgcatc ttaacaagat tggaaaagca
     3721 caggcagtga atcagctgca tttgggcgga ggcacaccga ctttcctcaa tgatgaagag
     3781 ttgtccagcc tcatgcagat gctgaaaaca gcttttgtat tcagtgagga aggcgaatac
     3841 tccatcgaaa ttgacccgcg gaccgtgaat caggtccgtt tgcaacactt gcgtgacctc
     3901 ggttttaacc gcctgagttt tggcgtgcag gatttcgatg cagaagtgca gcaggccgta
     3961 caccgggtac agtcttatac gcaagtcgaa accctgatgc aacaggcgcg gcagatgggc
     4021 taccagtcta tcaatgccga cctgatttat ggtttgccca agcaaacgcc agcgactttc
     4081 cgcgaaacca tcagtaaaat tactcaattg cgtccagacc gcattgcctt gtatggctat
     4141 gcgcacctgc cttcacgctt taagccacaa cgccgcattt tgcaggagga cctgccgaat
     4201 gcagtcgata aaatccagat gctatcagaa gccatccagc aattgatgca ggcgggttat
     4261 gtgtacatcg ggatggatca ctttgccttg ccgactgatg cgctggcgat tgccaagcag
     4321 cagggcaggt tgcaccgcaa tttccagggc tacagcacca tggccgacca ggatatcctg
     4381 gcactgggtg tctctgctat cggcaaaatg ggcgccacct acagccagaa tgccaaaacg
     4441 ctggaagagt attacgactt tcttgatcaa gggcagttgc ccatcgtacg cgggctggca
     4501 ttgagccggg atgacctgat ccgccgtgcc gtgatcatgg caattatgtg ccatggtgaa
     4561 ctcagctatg aatctattga tctttcctat atggtgaact tcagagatta ctttaagtca
     4621 gaaatgcaac aacttgttga aaaagaaaaa caaggcctgg tgattctgga agatgacggc
     4681 ctcaaagtca ccgaaatggg ttggtatttt gtacgtgcga tagccatggt gtttgaccgc
     4741 tatttgcagc acgatgccaa tcgtgcaagg ttctccaaaa tcctttaaac tgccggcatg
     4801 ttagccagct tactcctgac ggccttcgtc atggggctgg caggcggccc gcattgtatt
     4861 gccatgtgtg gcgcagcttg cggcgccatg cagtcctcct ctcaaacgac cagccagttt
     4921 cgttccatcc cattgcgagt ggttaaaacc atcccctgga gcttttacct gggacggatg
     4981 ctcggttacg gcctgttggg cgcgttggca gcagaaaccc tgcgcggcct ggcctggctt
     5041 tctaaccaga gtcatgtttt tcaaccttta tggacctttt cccataccct gatttttgcc
     5101 tggggtgtgt tattgctggt gacaggtaag caaccgcaat gggcgataaa acgtgcgcaa
     5161 caagtctggg tctggatcaa gcaacgtaca caacatgaca gggtaggctt cctgggcact
     5221 ggtatggtct gggcgttatt accgtgtggc ttgttatatt cggccgtcat gctggcgtct
     5281 ttgcaatcac agtggttgca aggtgcattg gtcatgatgg catttgcggc tggctctgcc
     5341 actgtcttgg ccattgcgcc attggtatgg cggtctgggc aaggcgcgtt gccaaactgg
     5401 atgcgtgaat ctacaggcat gcggctcagt ggtatttttc tcatcagctt aagcctgatt
     5461 gcattatgga tggacctcat gcatcacgat aagttatggt gccaagtact atgatttata
     5521 aatgaaaagt aattaattta taaaacttat tctttcatcc ttccttgccc aaaatatcaa
     5581 tctgatgtag cttac
//
LOCUS       DATMSK010000161         8095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_129206,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000161 DATMSK010000000
VERSION     DATMSK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129206"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..288)
                     /locus_tag="VNZ84_05410"
     CDS             complement(52..288)
                     /locus_tag="VNZ84_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU66427.1"
                     /translation="MQNDSKLSLAVRESLDDYFTHLDGQPPHAIYDMVLGCVEKPMLE
                     YVLNKVGGNQSKAADMLGINRNTLRKKMATYGLE"
     gene            complement(288..1316)
                     /gene="dusB"
                     /locus_tag="VNZ84_05415"
     CDS             complement(288..1316)
                     /gene="dusB"
                     /locus_tag="VNZ84_05415"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HWU66428.1"
                     /translation="MQIGSHQLKNNLVVAPMAGVTDRPFRMLCKKMGAGLAVSEMVTS
                     NSLLYGSEKTKRRANHEGEVSPISVQIAGAEPAMMAEAARHNVENGAQIIDINMGCPA
                     KKICNVMAGSALLRDEPLVSQILKAVVNAVNVPVTLKIRTGWDRQNKNAIQIARIAED
                     IGVQALTIHGRTRNDLYHGDAEYDTIAAVKQAINIPLIANGDITTPEKAKFVLDYTKA
                     DAVMIGRAAQGRPWIFRETEHFLKTGEHMLPPTVDEIHQVMLEHLHDLYAFYGDLTGM
                     RVARKHISWYTKGLSGSAAFRHNMNTLQTIELQLQAINDFFAELRAKNDRLVYADNEE
                     SSDEELAA"
     gene            complement(1396..2022)
                     /locus_tag="VNZ84_05420"
     CDS             complement(1396..2022)
                     /locus_tag="VNZ84_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWU66429.1"
                     /translation="MVRKTKEDAAITRQRIIDAAREMFLLKGVSRTSLEQIASHASVT
                     RGAVYWHFQNKAELFHAMREEVYLPLIDRMDDTLLGNTDDPSPDPLGRIRKHLESTIQ
                     ILDDDQTTRATYEVMMTKCEYVDEFADVLQSILSNCSGLVHKMEQAYAKAQEIGQVGT
                     QLSAAELALDTHLFFSGLLHMWVKDTEGTLFRNRALQLIDTHIKLRKK"
     gene            2207..3388
                     /locus_tag="VNZ84_05425"
     CDS             2207..3388
                     /locus_tag="VNZ84_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU66430.1"
                     /translation="MLISQTIQPRHTLLVLALGVVLSACGKSSNEQNPAAAGMPAMPV
                     TVQAVAAETVPIQTEVVAQTEGAKQIEVRPRVGGIVLKRLYQEGETVKAGQDMFLIDP
                     VPFQLQVDQAKALIAQQKARIEQTAREALRLKGLLDSKSISQREYDNAVSDSSVATAT
                     MLQYQAQLREAELNLSYTHVKAPESGIGGRFVLSEGALASANTTLLSTIVQVSPIWVR
                     FSLSESELQALGGHLRPGKVQDVRLILADESEYPLSGKINFSASQIDPALGTQQLRAE
                     FQNPDSALLPGQFVRVRLTTGHRDGVFLIPQTAVLTGQQGKFVFVADKDKEGKTVAAV
                     RPITVGGWFGERWSVLSGLKAGDEVITDNLIKVRPGAPVAPHAPDAAPAGAPGQDKPK
                     S"
     gene            3463..6597
                     /locus_tag="VNZ84_05430"
     CDS             3463..6597
                     /locus_tag="VNZ84_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWU66431.1"
                     /translation="MTRFFITRPIFASVLSIIIVLSGLAAAFKLPIAQYPQIAPPTVI
                     ITATYPGASAETLSKTVAAPIEEQLSGVENLLYFNSSSDSSGTLTITATFDIGTNVDQ
                     ATFNVSNRVNIALPRLPEDVRRTGVVVQKRSNDILLVVMLISSKKEHDRLFLSNYATL
                     NVLDEFKRTKGVGDVTIFGGQDYSMRIWLRPDRMAQLGVSTSDISAAISSQNAQYAAG
                     KIGAEPAPASQQLSFTVTAKGRLIDPEEFGNIIVRAQGPNGVLRLKDVARIELGAQSY
                     NVASALNGQPGVALPIFLQSGANALETAEAIKAKVNELKAKFPEGMDYNIPYDTSDFV
                     KATIKEVFHTFGEALILVVLVVFLFLQSWRATLIPMIAVPISIIGTFAGLYLFGFSIN
                     LLTLFAMILAIGIVVDDAIVVLENTERLMKEENLNPLHAAIKSIAQVQAAVVAIVLVL
                     CAVFVPVAFQGGIAGELYRQFAVTVAISVVISGVVALTLTPALCAMILKNGHHENAFF
                     RKFNQGFERLTKLYTGTVNLTLHHKIIGAVVFGGMLIVMILLFRTVPGGFVPPEDQGY
                     VISIVVMPDGATLRRTSQTTENIRAAIAKDPATAHEFAVNGFELLTGANKTNAATMFV
                     RMTDWDKRTTNADQMVGKLIGIGMSQPDGLSFAVNPPAIRGLGSAGGFEVYVQSKGDT
                     DPIKLAQVMNNFMDAMRADSKLTGINSFFRPTVPQLFIEVDEAKALSQNVKISDIYAT
                     LQSTMGSLYVNDFNRSGRTYRVQLQAEAEYRMKPEDLGKVYVRSQPTADNPDGNMIPM
                     SALAKVSNIVGAEQLERYNGLLSAKVFGSGAAGVSSGDAIKLVEKIAKESLPDGYQIA
                     WTGQAYQEKRTGSAALVAFGFATIMVFLILAAQFETWALPLAVIMAIPFAMTGALIAV
                     LTRGMNNDIYFQIGLITLIGLAAKNAILIVEFASQKMEEGMPLAEAALEAARLRFRPI
                     VMTSMAFVLGIIPLVFATGAGAAARQSMGTGVFGGMLLATFIATIFIPLFFTWLTNEK
                     KVRHHGHIVEEESV"
     gene            6594..8078
                     /locus_tag="VNZ84_05435"
     CDS             6594..8078
                     /locus_tag="VNZ84_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWU66432.1"
                     /translation="MKPTMKFALKPLQQSLLLGLLTLSMTGCQMLGDLYSRPAPQLPE
                     KYGTANTGVSDPAQQVALQQWWTLFNDSQLNQLVETALVKNNNVQLAVARIEEADAQM
                     REIGGNLLPTITATSSGIRNRVTQSGVFPVFGNNPRKTYKIGLNGSYELDLWGKLRRA
                     NEAARAQLLATQYAKEAVTWSLSSLVANNYLAIRSLDAQLRVNEENLKTAQESLDLAK
                     RRVEGGVATVLDAHQAELVVTTLQAQIVDLKRLRALSEHQLGLLTNNLGLKVAPGDLM
                     AIPTPPVVPTGLPSELMEARPDVRQAEQDMVSANANIAVARAAFYPSIALSATYGGES
                     IALNNLLKSPARVWSLGFDINLPIFNGGRLNARLDQATAQQKQMVATYQNTLQTAFTE
                     VSDALNNVQQYREQEALADSKQKTTGNILRVAKNRYEAGYTSYLEVLDAQRNHNEATQ
                     AMVQSRQNTLTASVDLFKALGGGWKPESLADQKVSKADEKVSKK"
BASE COUNT         1897 a         1892 c         2220 g         2086 t
ORIGIN      
        1 gtgataaaaa tgaaaaggtc taatgacgca tgtcatcaga cccggttaag tctattcaag
       61 gccataggtg gccatttttt ttctcagggt gttgcggtta atgcccagca tgtctgccgc
      121 tttactttga ttgccgccta ccttgttgag cacgtactcc agcatgggct tttctacgca
      181 gccgagtacc atgtcgtaaa tggcatgtgg tggttggccg tccaggtgcg tgaagtaatc
      241 atccagagat tcgcgaacgg cgaggctgag cttgctatcg ttttgcatta tgcggccaac
      301 tcctcgtcac tgctttcttc gttgtcggca tagaccaagc ggtcattttt ggcacgtagc
      361 tcggcaaaaa agtcattaat ggcctgtaat tgcaactcta tagtctgaag ggtattcatg
      421 ttatggcgga aagcggcgga gcctgacagt cctttggtgt accaggaaat atgtttacgc
      481 gccactcgca tgcccgtgag gtcgccataa aaagcgtata gatcatgcag gtgctccaac
      541 atgacttgat ggatttcatc tacggttggc ggcagcatat gttcgccggt tttcaggaag
      601 tgttctgttt cacggaaaat ccatggcctg ccttgcgctg cgcgccctat catcacggca
      661 tccgccttgg tgtaatcgag tacgaatttg gctttttccg gcgtggtgat gtcgccgttg
      721 gcaatcaacg gaatgttgat ggcttgttta actgcagcaa tcgtgtcgta ttcggcgtcg
      781 ccatgataaa gatcgttacg ggtgcggcca tgaattgtca acgcttgtac gccaatatct
      841 tcggctattc tggcaatctg gatggcgttt ttattttgtc tgtcccagcc ggtgcggatt
      901 ttcagagtca caggaacgtt tactgcgtta actactgcct tgagaatttg tgataccagc
      961 ggctcatcgc gcaacaaggc tgagcctgcc atcacattac agatcttttt ggccggacag
     1021 cccatgttga tgtcaatgat ttgtgcgcca ttctcgacat tatgtctggc ggcttccgcc
     1081 atcatggctg gttctgcgcc agcaatctgg actgaaattg ggctgacttc cccttcgtga
     1141 ttggcgcggc gcttggtttt ttcactgccg tagagtaatg agttggacgt gaccatctcg
     1201 gacacggcca gtccagcccc catctttttg cacagcatgc ggaaaggcct gtcggtcacg
     1261 ccagccatag gggcgacgac caaattattc ttgagttggt gcgatccgat ttgcatgatg
     1321 tggataagtc agtgaaaaga ctgactattt taactgcaaa acgcggatca gggaatccgt
     1381 aaaatgaatt attgcttatt ttttacgcag tttgatgtgt gtatctatga gctgtaaggc
     1441 gcgattgcgg aagagtgtgc cctcggtgtc tttgacccac atatgcagta ggccggagaa
     1501 aaataaatgc gtatcgagtg ctagctctgc tgccgagagt tgggtgccta cctggccaat
     1561 ttcctgggct ttggcataag cctgttccat tttgtgtacg agtccgctac agttacttaa
     1621 aatagattga agcacgtcgg caaactcatc gacatattca catttggtca tcatgacctc
     1681 ataggtggca cgcgtggttt ggtcgtcgtc gaggatttgt atcgtgcttt ccagatgttt
     1741 gcggatgcgg ccaaggggat ctgggctagg gtcatctgta ttgccgagca gcgtgtcatc
     1801 catacggtcg attaaaggca ggtagacctc ttcgcgcatg gcatgaaaca gttctgcctt
     1861 gttttgaaag tgccagtaaa ctgcgccgcg ggtcacgctg gcgtgtgagg caatttgttc
     1921 caggctggtt cggctcacgc ctttgaggag gaacatttcg cgcgccgcat caatgatgcg
     1981 ctggcgggta atggcggcat cttctttggt tttacgtacc ataagtctct atcgctcttt
     2041 cagatttcat taaagtttct tgtagcttgt gtattttacc ggtagtgcca ggtggctttt
     2101 tcggcaaaat ttttacaaac cccatttaca tacatacatg tttgtatata atatacatcc
     2161 aagaatgtat gtaatcaagt ctaatttaaa tttttttgag gataacatgt tgatttcaca
     2221 gactatccag cctcgtcata ccttgctcgt acttgctttg ggcgtcgtat tgagtgcctg
     2281 cggaaaatca tccaatgaac aaaacccagc tgcggctggc atgccagcga tgccagtgac
     2341 cgtgcaggcg gtggccgcgg aaacggtgcc aattcagact gaagtggttg cgcaaacaga
     2401 aggcgccaag cagattgaag tccgtccgcg cgtgggtggc attgtgctca agcgcctgta
     2461 tcaggaaggt gaaacggtga aggctgggca ggatatgttc ctcattgatc ctgtgccttt
     2521 ccagttgcag gttgatcagg cgaaagcatt gattgcacag caaaaagccc gcattgaaca
     2581 aactgcgcgc gaagcgttac gtttgaaggg gttgctggat agtaaatcca ttagccagcg
     2641 tgaatacgat aatgccgttt cagatagttc agtggcgacc gcaaccatgt tgcagtatca
     2701 ggcgcaactg cgtgaagcag agctgaattt gtcttatacc cacgtcaaag cgccagaaag
     2761 cggtattggt ggtcgttttg tcttgtctga aggggcattg gcaagtgcta acacgacctt
     2821 gttaagtacc attgtgcaag tttcccctat ttgggtacgg ttcagtttgt ctgagtccga
     2881 gctgcaggca ttgggcggtc atttacgtcc aggcaaggta caggatgtac gtttgatcct
     2941 ggcggatgaa agcgagtatc cgttgtcagg aaaaatcaac ttttctgcca gccagattga
     3001 tccagccttg ggcacacagc aattgcgtgc cgaattccaa aacccggata gcgccctgct
     3061 gccaggccag ttcgtacgtg tacgtttaac gaccggtcac cgtgatggcg tgttcctgat
     3121 cccgcaaacg gccgtgttaa caggccagca aggcaagttt gtatttgtgg cggataagga
     3181 taaggaaggt aaaaccgtag cggccgtcag acccattact gtcggtggct ggttcggcga
     3241 acgttggagc gtgttgagtg gtttgaaggc aggtgatgaa gtgatcactg ataacctgat
     3301 caaggtacgt ccaggcgccc cggtcgcgcc gcatgcacca gatgccgccc cggctggagc
     3361 acctggtcag gacaagccca agtcttaata cctctttatt acgttgatat tatgttattc
     3421 acggcctgag cgtcgtttca ggccgatttc aggaaaaacg atatgacccg attttttatc
     3481 acacgcccga tttttgccag cgtgctgtct atcatcatcg tgttgtccgg tttagcggct
     3541 gcattcaagc tgccaattgc gcaatatccg caaattgcac cgccaaccgt gattatcacc
     3601 gccacttatc caggtgccag tgctgaaaca ctgtccaaaa ccgtggctgc cccgattgaa
     3661 gagcagctca gtggtgtgga aaacctgctg tactttaact caagctcaga cagtagtggc
     3721 acgctgacaa tcacagcaac ctttgatatc ggcaccaatg ttgaccaggc gacttttaac
     3781 gtcagtaacc gcgtgaacat tgcgctgccg cgtttgccag aggatgtacg ccgaactggt
     3841 gttgtcgtgc agaaacgttc taacgacatc ctgttggtcg tgatgctgat ttccagcaaa
     3901 aaagaacatg accgcttgtt cctgagcaac tacgcgaccc tcaatgtcct ggatgagttt
     3961 aaacgcacca aaggtgtggg tgatgtgacc atttttggtg gccaggatta ctccatgcgt
     4021 atctggttgc gtccagaccg tatggcgcag ttaggggtat ctacctctga catttctgcg
     4081 gcgatcagtt cgcagaacgc gcaatatgca gcaggtaaga ttggcgcgga accagccccg
     4141 gccagccagc aattgtcatt tactgtgact gctaaaggcc ggctcattga tccggaagag
     4201 ttcggcaata tcatcgtacg tgcgcaaggc cctaacggtg tacttcgcct gaaagatgtg
     4261 gcgcgtatcg agctgggtgc acagagctat aacgtggcat ctgccttgaa tggacagcca
     4321 ggggtggcgt tgcctatctt cctgcaaagt ggcgccaatg cactggaaac cgctgaagcc
     4381 attaaagcaa aagtgaatga gttgaaagcc aagttcccag agggtatgga ttacaacatt
     4441 ccttacgata ccagtgactt tgttaaagca accatcaaag aagtgttcca cacctttggt
     4501 gaggcgctga ttctggtggt gctggtggtg ttcctgttcc tgcaaagctg gcgtgcgacc
     4561 ttgatcccta tgattgcggt gccgatttcg attatcggta cgtttgccgg cttgtatctg
     4621 tttggcttct ccatcaacct gctgacgctg ttcgccatga ttctggcgat tggtattgtg
     4681 gtggatgacg cgatcgtggt gctcgagaac accgagcgac tgatgaagga agaaaacctg
     4741 aatccgctgc atgcggcgat taaatcgatc gctcaggtac aggcagcggt ggtggcgatt
     4801 gtgctggtat tgtgcgcggt atttgtaccg gtcgctttcc agggtggtat tgccggtgag
     4861 ttgtaccgtc agtttgcggt cactgttgcc atttctgtgg tgatttcagg tgtggtggcg
     4921 ttgacgctga cccctgcttt gtgcgcgatg atcctgaaaa atggccatca tgaaaatgcg
     4981 ttcttcagga aattcaacca gggctttgaa cgtttaacca agctgtatac cggcacggtg
     5041 aacctgacgt tgcatcataa aatcattggt gccgtcgtgt ttggcggcat gttgatcgta
     5101 atgatcctgc tgttccgtac tgtaccaggt ggttttgtgc cgccggaaga ccaaggctat
     5161 gtgatcagta ttgtggtgat gccggatggc gcgaccttaa gacgtacctc tcaaaccaca
     5221 gaaaatatcc gtgcagcgat tgccaaggac ccagccactg cgcatgagtt tgcagtgaac
     5281 ggctttgagt tgctgacagg tgcgaataaa accaatgcgg ccaccatgtt tgtgcgtatg
     5341 acggactggg ataaacggac caccaatgct gaccagatgg tgggcaagtt aattggtatt
     5401 gggatgagcc agccagatgg tttaagtttt gccgtgaacc cgccagcgat tcgtggtctg
     5461 ggttctgcgg gtggttttga agtttatgta caaagtaagg gtgatactga tccaatcaag
     5521 ttggcgcagg tcatgaataa cttcatggat gcaatgcgtg cagactctaa attgaccggt
     5581 attaacagct tcttccgccc aacggttccc caattgttta ttgaagtgga tgaagccaag
     5641 gcgctgtcac aaaatgtcaa aatttcagac atttatgcca ctttgcagag caccatgggt
     5701 tccctgtatg tgaacgactt taaccgtagt ggccgtacct atcgtgtgca gttgcaggct
     5761 gaagccgagt accgcatgaa gcctgaagac ctgggtaaag tgtatgtacg cagccaacca
     5821 acggcagata atccggatgg caatatgatc ccgatgtctg cattggctaa agtcagcaat
     5881 attgtcggtg ccgagcagtt ggagcgttac aacggcctgc tgtctgccaa agtgtttggt
     5941 agtggcgcgg ctggcgtgag ctcgggtgat gcgatcaagc tggtggagaa aattgccaag
     6001 gaaagcttgc ctgatggata ccagattgca tggacaggcc aggcttacca ggagaagcgt
     6061 accggttctg cggcattggt agcgtttggt tttgcgacca ttatggtgtt cctgatcctg
     6121 gcagcgcagt ttgaaacctg ggccttgcca ctggcggtga ttatggcgat tccgtttgcc
     6181 atgaccggtg ccttgattgc agtgctcaca cgcggcatga acaatgatat ttacttccag
     6241 attggcctga tcacgctgat cggtctggca gcgaagaacg cgatcctgat cgtggaattt
     6301 gcgtcacaga aaatggaaga gggcatgcca ttggctgaag ccgcattgga agctgcacgt
     6361 ttgcgtttca gaccgattgt gatgacatca atggcgtttg tattggggat cattccactg
     6421 gtattcgcca ccggggccgg tgcggcagcg cgtcagtcca tgggtaccgg tgtgttcggt
     6481 ggtatgttgc tggcaacgtt tattgccacc atctttatcc cattgttctt tacctggttg
     6541 accaatgaga aaaaagtacg tcaccacggt catattgtag aagaggaatc ggtatgaaac
     6601 cgacaatgaa atttgcatta aaaccgttgc agcaatcgtt attgctgggg ttgttgacct
     6661 tgtccatgac aggctgccag atgctgggtg atttgtattc ccgtccggca ccgcaactgc
     6721 cggaaaagta tggcacggcc aatactggcg tgtctgaccc agcacagcaa gttgccttgc
     6781 aacaatggtg gaccttgttt aacgacagcc agctgaacca gctggtagaa actgcgctgg
     6841 tgaaaaacaa taacgtgcag ctggccgtag cgcgcattga agaagccgat gcccaaatgc
     6901 gagagattgg cggtaacctg ttgcccacga tcactgcgac gagttccggg atacgtaacc
     6961 gtgtgacaca gtcaggtgtg ttcccggtgt ttggcaataa cccgcgtaaa acctacaaaa
     7021 ttggtttaaa cggtagttat gagctagacc tgtggggcaa gctgcgtcgt gcaaatgagg
     7081 cggcacgtgc gcaattgctg gcgacacaat atgccaaaga agcggtaacc tggtcgttat
     7141 ccagcctggt ggcgaacaat tatcttgcga tacgcagcct ggatgcccag ttacgggtga
     7201 atgaggaaaa ccttaagacc gcgcaagaga gcctggactt ggccaaacgg cgcgttgaag
     7261 gtggtgtggc gactgtgctg gatgcacatc aggctgagct ggtcgtgacc acactgcaag
     7321 cgcagatcgt ggacctgaaa agattgcgtg ctctaagcga gcatcaactg ggtttactga
     7381 ctaacaacct cggcttgaag gttgcgccag gcgacctgat ggctatccca acaccaccag
     7441 tcgtaccgac cggtctccct tcagaattga tggaagccag acctgatgta cgtcaggcgg
     7501 agcaggatat ggtatcagcc aacgccaata ttgccgtggc ccgtgctgct ttttacccta
     7561 gtattgcgct ctcagccacg tatggtggcg aaagtattgc cctgaacaat ttgctgaaat
     7621 ccccggcacg tgtctggtcg ctcggttttg atataaacct gcctattttc aatggcggac
     7681 gcctgaatgc acgattggat caggccactg cgcaacagaa acaaatggtg gcgacgtatc
     7741 aaaacacctt gcaaactgcc tttactgaag tgagtgacgc actcaacaat gtgcagcaat
     7801 accgagaaca agaggcattg gcagactcca agcaaaaaac cacagggaat atcctgcgtg
     7861 tagcaaaaaa ccggtatgaa gcgggctata ccagctatct ggaagtgctg gatgcacagc
     7921 gcaaccataa tgaagcaacc caggccatgg tgcaaagccg tcaaaacaca ttgaccgctt
     7981 cggttgattt atttaaggca ttgggcggtg gctggaaacc ggaaagcctg gcagatcaga
     8041 aggtctctaa agctgacgag aaggtttcta aaaagtaatt ttgtcaaagt gtatg
//
LOCUS       DATMSK010000162         7977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_434641,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000162 DATMSK010000000
VERSION     DATMSK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434641"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="nadA"
                     /locus_tag="VNZ84_05440"
     CDS             <1..929
                     /gene="nadA"
                     /locus_tag="VNZ84_05440"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWU66433.1"
                     /translation="KLSKYCDSLDAEYIVFLGVHFMAEVADILSRPEQIVILPDLAAG
                     CSMADMANLAKVERSYRELNKVLNFDETITPVTYINSAADLKAFCGEHGGIVCTSTNA
                     PKILEWGFSQREKILFFPDQHLGRWSGHKMGIPLEQMPVWDPDLPMGGLTEQQIKDAK
                     ILLWKGHCSVHQMFRKQNIDQFRAQHPDGKVISHPETAFEVCINSDYVGSTEYILRTV
                     SEAEPGSKWLVGTELNLVTRLAEEMKPQNKLVQFMSHVVCECSTMARIDPQHLAWCLE
                     NLAEGNVVNQIKVPEHEAKLAKLTLDRMLAVS"
     gene            929..1357
                     /locus_tag="VNZ84_05445"
     CDS             929..1357
                     /locus_tag="VNZ84_05445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HWU66434.1"
                     /translation="MSDCPLCQPTPHDLLWQDEFCRVVLLHDSDYPAYCRIELLAHVK
                     EMTDLAPADRARTMKVVFAVETAIREVIQPDKINLASLGNKTPHMHWHVLPRFESDKH
                     FPNSHWGEPMRDSGVQPLSQPVRDQLQKIVIERVECALKS"
     gene            complement(2081..5152)
                     /locus_tag="VNZ84_05450"
     CDS             complement(2081..5152)
                     /locus_tag="VNZ84_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU66435.1"
                     /translation="MNFSAWSIRNPVPAILLFVVLSFLGLKSLNQLGKQNFPDIELPV
                     INVSATLEGAAPPQLETEVARKIEDKIATIGGIEHMTTTITDGSVSISVQFTIDTNSE
                     EALNLVRNAVDSARSELPAAVTTPTVSKVTTSGNAILTFVASADNMDEGELSWFVDNE
                     VSKAMLAVNGVGKITRTGGVDREVQVNLDPTLMAGLEVNVGDVSAQLKRVQQDASGGR
                     GDIGDNIQSVRTLGALHSADEIRNLDIPLGDGRHIKLSQVAEVLDTYAERTTYASYDG
                     KPVIRFEITRSKGTSEVTVAKDVRAALEKFEQLHPQVKIVEAFNMVKPVEDNYEGSMH
                     LLYEGALLAILVVWWFLRDWRATIIAAVALPLSMIPTFAFMQYFGFSLNVLTLLAMAL
                     VVGILVDDAIVEIENIVRHLRMGKTPYQAAMEAADEIGFAVIATTFTLVAIFLPTAFM
                     GGIPGKFFKQFGITAAIAIMGSLIVARLLTPMMSAYLLKPHPLKEESDSTSMRVYLQW
                     VSWCLKHRKTVVAGFILFIVGSLSLMPLLPKGFVPSSDSSQTKVNIELQPGTPLEQTR
                     QVVQMAEQIVKQHPEVKAVFSAAGTASTGGGGPNASQNSTDVRKGSLVISLTDRSERK
                     QKQSEIEADLREQLKNLPGARVSVGIGESGEKLQITLASDDAIALTNASQQLQQQLRT
                     LNGVGNVTSSASLQRPEVQIIPDIDKAAELGVTVESIAQVIRVATSGDFTNTMPKLNL
                     PQRQVPIRVRLGPSVRTSLQEIAQLRVPSRNGSVSLETVATVRMGSGPQQIARMDRMR
                     NVTFDIELNKRQIGEVMREVQQLPAMQNLPPSVKQLEAGDAKRMNELFASFGGAMIIG
                     VMCIYVVLVLLFGDFLQPVTILGALPLSLGGAFFALLVTHNSFSMPSVIGLLMLMGVV
                     TKNSILLVEYTIMARKERKLNRFDAIIDGCHKRARPIIMTTIAMAAGMLPIALGWSAD
                     PSFRSPMAITVIGGLITSTVLSLVVIPVLYTYVDDLLGWLRGLGRLARKHV"
     gene            complement(5149..6345)
                     /locus_tag="VNZ84_05455"
     CDS             complement(5149..6345)
                     /locus_tag="VNZ84_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU66436.1"
                     /translation="MAKKLTKKTKFLIIAALLVGVSAWFWQHPLHQAELAEKRAGRQP
                     VAKPVTDNKTSTPAQAVKAALSVEVTQPAVVQWPMTLVLSGSIYPWQEALVSAEISGL
                     RIQQILADVGTQVSKGQPLVMLADETVKADLQKQLATVDRDKAALAEAKSNADRAREI
                     QNSGALSAQKINEYVIAEQTAKANLALSEAELENQKIRLRQTKVVAPDDGVISSRSAN
                     LGNVVSSGAELFRLVRQGRIEWRGEVNADQQTALRAGQSVRLTLNGGNSVNGKVRLIS
                     PTVDNNTRNALVYVDIPKDSAKPGMYVQGQVDIGQQQGLAVPLSAVTYRDGFAYVFEL
                     AAADAQGLHKVAQRKVQTGRTRGEQIELIGNLSRDAKLVLSGGAFLNDGDIVKVVTVN
                     KRGASV"
     gene            complement(6351..7772)
                     /locus_tag="VNZ84_05460"
     CDS             complement(6351..7772)
                     /locus_tag="VNZ84_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWU66437.1"
                     /translation="MGLGGLLLCLSLTGCSVIDDYIRPKPATAEQWQAPLPHGGSVTS
                     LKDWWGQFNDPVLDQLLAESQKESPSLEIALANIRVARANVLSARAQGIPDLSSTGSM
                     TKSKGGGGGAFPSSTIEIDSVSLDASWEVDLFGKVKAAKQAAKAQLEAKKIAWHDARL
                     SLAAEVANNYVNYRACQVSVQALKQAYDSRSETARLTGISANAGFSAPSDLALAEAAT
                     RASESTLDGQQAECDGLVKALVELTNLPEPELRNLLAKGHGMPEPAMFDVNAMPANLL
                     TRRPDLAVDERNLAEASANIGVSKAQMYPSLTLTGSIGYRRTNFNGAVTRTDSWSYGP
                     SLKLPIFDGGTLRADLAEARANFDSLLATYKQDVRKAVKEVEQALVNLDSATRRAQSE
                     QASAQQYQQYFKAAEINWKSGGLDLLSLEDARRQKINAELNVITQQKNRVLQWIALYK
                     AFGGDWQETQQTVKPPLKPMEKR"
BASE COUNT         1960 a         2221 c         1896 g         1900 t
ORIGIN      
        1 tgaaactatc caaatactgt gacagcctgg atgccgaata catcgtgttc cttggtgtgc
       61 actttatggc agaagtggcg gatattctta gccgcccaga acaaattgtg atcctgcccg
      121 acctcgctgc cggttgctcc atggcggata tggccaacct ggccaaggta gagcgtagtt
      181 accgcgagct gaataaggta ctcaactttg atgagaccat cacgccagtc acctatatca
      241 actcggcggc tgatttgaaa gcattctgtg gtgagcacgg cggcattgta tgcaccagta
      301 caaatgctcc gaaaattctg gaatggggtt ttagccagcg cgaaaaaatc ctgttcttcc
      361 ccgatcagca cctgggtcgc tggagcggcc ataaaatggg tatccctctg gagcaaatgc
      421 cagtatggga tccagacctg ccgatgggtg gcctgacaga acaacagatc aaggatgcca
      481 aaatcctgct atggaaaggc cattgctccg ttcaccagat gttccgcaaa cagaatatcg
      541 accaattccg cgctcagcat ccagatggca aagtgatctc tcacccggaa acggcatttg
      601 aagtctgtat caactccgac tatgtcggct ctaccgaata tattctgcgc accgtcagcg
      661 aagctgaacc gggtagcaag tggctggtgg gcacggagct caacctggtc acccgcctgg
      721 ctgaagaaat gaaaccacaa aacaaactgg tgcagtttat gagccatgtc gtctgtgaat
      781 gttcgaccat ggcccgtatc gacccgcaac acctggcctg gtgcctggaa aacctggctg
      841 aaggcaatgt ggtcaaccag atcaaagtgc cagagcatga agctaaactg gctaaactca
      901 cactggaccg tatgctggca gtctcataat gagtgactgc ccactttgcc aaccaacgcc
      961 gcacgacctg ttatggcaag atgaattctg ccgcgtggtg ttactgcatg attcagatta
     1021 tccggcctac tgccggatcg aattactggc ccatgtgaaa gaaatgacag acctagcgcc
     1081 tgcagaccgc gcgcgaacca tgaaagtggt gtttgcagtc gaaacggcaa tacgtgaggt
     1141 catccagccg gacaaaatca acctggccag tttaggcaat aaaacaccgc atatgcattg
     1201 gcatgtatta cccaggtttg agagtgacaa gcacttccct aatagccatt ggggagagcc
     1261 tatgcgggat tccggggtgc agccattaag ccaaccagtg agagatcaat tacagaaaat
     1321 cgtaattgag cgcgtggaat gtgcgcttaa gtcctgatgt tttgaaactc tacttctgtt
     1381 attaactcga caaatttatt tcgcctctcg acgagttact ttctggtgct tgtcccacag
     1441 aaagtaacca aagaggacga cacccagcca tcacggcctt gcggctccct tgcgttgctc
     1501 aacaaagtaa gcggtcccga aactcgccct agcagcttga caccccgcaa gcttctgcgg
     1561 agctcagaca gtcgctcctt tacttcttac tttgtttccg ctactcagcg tgatgggatg
     1621 ggatttgtct cgccaaactt acagcgtaaa agattgaacg tttaaaaact caaattcttt
     1681 tctttgatcc actaccagtg gcgctaacga gtttttgggg cccctatcta ccgcgccgag
     1741 tagcgcaggc aaaataagat gaagcgagcg gctgttcgaa tcccgcggtg gcctgcgaag
     1801 tatgcaggcc gctaggatga gtttcgcgag cgcttatttt ggtgagcaac acaggaaaca
     1861 agcggaagct agggtgcatt tcttttggtt acttattctt tgggcaaaca aagaaaagta
     1921 actcgcctag aggcgaaata gaaagattgt aagttgttaa gtgacgatac gagtggcact
     1981 acaaccaaaa atttaaagaa ttaccttctt atacatccaa taaataacga ggcgcgcctc
     2041 tcaacacgcc tcgttttaag tcacctgcat tttatagaga ttacacgtgc ttgcgcgcca
     2101 atctccccag acctcgcaac cagcctaaca aatcatccac ataggtatac aacacgggga
     2161 tcactaccag acttaacacc gtagatgtaa tcaacccacc aatcaccgta atcgccatcg
     2221 gcgaacggaa actgggatct gcactccatc ccagcgcaat gggcaacatg cctgctgcca
     2281 tggcaatcgt cgtcatgata atcggccgtg cacgtttatg gcagccatca atgatcgcat
     2341 caaagcggtt gagcttacgt tccttacgcg ccatgatggt gtactcaacc agcagaatag
     2401 agtttttggt gaccacgccc atcagcatca acaagccaat cacacttggc atggagaaac
     2461 tgttatgcgt caccaggagc gcgaagaaag caccgcccaa cgacaacggt aatgcaccca
     2521 aaatagtaac cggttgcaaa aagtcgccaa acaacaatac cagcacgacg taaatgcaca
     2581 tgacaccaat aatcatggca ccgccaaaac tggcaaataa ttcattcatg cgcttggcat
     2641 cgccagcttc gagctgttta acactaggtg gcagattttg catggcgggc agttgttgta
     2701 cctcacgcat gacttcccca atctggcgct tgttcaattc aatatcaaac gtcacattac
     2761 gcatacggtc catgcgcgca atctgctgcg ggccgctacc catgcgcaca gtagcaactg
     2821 tttccaggct gacactgcca ttacgtgatg gaacacgaag ctgtgcaatc tcctgcaaac
     2881 tggtacgcac actaggacct aagcgcactc taatcggaac ctggcgttga ggtaaattca
     2941 gcttgggcat cgtattggta aagtcccccg aagtcgccac ccgaataacc tgcgcgatgg
     3001 attccacggt tactcctaat tccgccgctt tgtcgatatc aggaataatc tgcacttctg
     3061 gccgttgcaa gctggcgctg gaagtaacat tgccaacacc gttcaaggta cgtaattgct
     3121 gttgcaactg ctggctggca ttggttaaag caattgcatc atcactggct agcgtaattt
     3181 gcaatttctc acccgactca ccgataccaa cagacacgcg tgcgccgggt agatttttca
     3241 attgctcgcg caggtcagct tcgatttcgg attgcttttg tttacgctcg cttcgatctg
     3301 tcaggctaat caccaaactg cctttacgta catcggtcga gttttgagag gcattggggc
     3361 caccgccgcc cgtgctggca gtaccggccg cactaaatac ggccttcact tcaggatgtt
     3421 gtttgacgat ttgctctgcc atttgtacga cttggcgtgt ttgctccagg ggtgtccccg
     3481 gttgcagctc gatattcacc ttggtctggc tactgtcaga ggaaggcaca aaccctttag
     3541 gcaataaagg catcaagctc agtgaaccga caataaacaa gataaaaccg gcaaccaccg
     3601 ttttacgatg cttcaagcac cagctgaccc actgcaggta aacacgcatg gaagtggagt
     3661 cgctttcttc tttgagcgga tgcggcttga gcagataggc ggacatcata ggcgttaata
     3721 atcgtgcgac gatcagggat cccataattg caattgctgc tgtaattcca aactgcttga
     3781 aaaacttgcc tggaataccg cccataaacg cggtcggtaa aaagatagcc accagcgtaa
     3841 acgtcgttgc aatcacggca aagccaatct cgtctgctgc ctccatcgct gcctggtaag
     3901 gtgttttacc catacgcaaa tgccgcacga tgttttcaat ttcaacaatc gcatcatcca
     3961 ccaggatacc cactaccaac gccatggcta gcaaagtcag cacatttagc gaaaagccaa
     4021 aatattgcat gaaggcaaaa gtagggatca tggacagcgg caacgccacg gcagcaataa
     4081 tggtggcgcg ccagtcgcgc aagaaccacc acacgaccag gatcgccaat aaggcacctt
     4141 catacaacag gtgcatagag ccttcgtagt tatcctccac cggcttgacc atattaaaag
     4201 cttcaacaat tttcacctgc ggatgtaatt gttcaaactt ttccagggca gcccttacat
     4261 cctttgccac cgtcacttcg ctggtgcctt tactacgcgt gatttcaaag cggatcacag
     4321 gtttgccatc gtagctggca taggtggtgc gctcggcata agtatccagg acctcagcca
     4381 cttgcgatag cttgatatgg cgaccatcac ccagcgggat atccaggttg cgtatttcat
     4441 cggcactatg caatgcccca agcgtccgca ctgactggat attgtcgcca atatcaccac
     4501 gcccaccgga agcatcttgt tgtacgcgct taagctgtgc agatacatcg ccgacattga
     4561 cttccagtcc tgccatcaag gtaggatcca ggtttacctg cacctcgcga tcaacgccac
     4621 cggtacgggt gatcttgcct acgccgttca cggccagcat ggctttgctg acttcattat
     4681 ccacaaacca tgataattcg ccttcatcca tattgtcggc tgaagcgaca aaggtcagaa
     4741 tggcattacc tgaagtggtc accttggaaa cggtcggggt tgtcactgca gcaggcagct
     4801 cggagcgggc actatcgacc gcattgcgca caagattcag tgcctcttcg ctattggtat
     4861 caatagtgaa ttgcacgcta atactgacgc taccatcagt gatggtagta gtcatgtgct
     4921 caatgccacc aatcgtcgca atcttgtctt caatcttgcg cgcgacctca gtctccagtt
     4981 gtggtggtgc agcgccttcc agcgtggcac tgacattaat caccggtaat tcaatatctg
     5041 ggaaattctg cttgcccagt tgattgaggg atttaagacc caggaaactc agtacgacaa
     5101 acagcaggat ggccggaacc gggttacgga tggaccaggc ggaaaaattc ataccgatgc
     5161 cccgcgttta ttgacggtca ccactttaac gatgtcgcca tcatttaaaa aagcgccccc
     5221 actcaacacc agcttggcat ccctactcag gttgccaatc aactcaatct gctctccgcg
     5281 tgtacggccg gtttgaactt tgcgctgggc gactttatgc aagccttggg catccgccgc
     5341 tgcgagttca aatacgtaag caaagccatc acgataggtc acagcactca gcgggacagc
     5401 aaggccctgc tgctggccaa tatccacctg tccttgcaca tacatgcctg gtttggcact
     5461 gtctttagga atatcgacat agaccagcgc attgcgcgta ttattatcca ccgtcggcga
     5521 aatcaggcgt accttgccat tcacactgtt tcctccattg agtgtcaagc gtacagactg
     5581 gccagcacgc aaggctgtct gctggtctgc attcacctcg cctcgccact caatgcgccc
     5641 ctggcgtacc agccggaaca actcagcccc tgaggagacc acattcccca gattggcaga
     5701 acgtgatgaa ataacaccat cgtcaggcgc taccaccttg gtttgacgca aacgaatttt
     5761 ctggttttcc agctcggcct cagacaaagc cagattcgcc ttggcggttt gctcggcaat
     5821 cacatactca ttaatttttt gcgcagataa tgcgccgcta ttttggatct cacgcgcacg
     5881 gtctgcatta cttttagctt cggccaaagc ggccttgtcc cggtctacgg tagccagctg
     5941 cttttgcaaa tcggctttta cagtttcgtc agccagcatg accaggggct gccccttgct
     6001 gacttgcgta cccacatccg ccaggatttg ctgaatacgc agtcctgaaa tttctgcact
     6061 caccaaagcc tcctgccatg gataaatact gccactcagg accagcgtca tcggccattg
     6121 cacaacggcg ggctgtgtca cctcgacact caaagccgct ttgacggcct gggcaggcgt
     6181 actcgttttg ttgtccgtga caggttttgc cacaggttgt cggcctgcgc gtttttctgc
     6241 cagctcggcc tggtgtaagg ggtgttgcca gaaccatgca ctcacgccga cgagcagcgc
     6301 cgcaatgatt aagaatttgg ttttttttgt cagttttttt gccatcatgc ttaccttttt
     6361 tccatgggtt tcaatggagg tttcacagtt tgctgtgttt cctgccagtc cccgccaaat
     6421 gccttgtata gggcaatcca ttgcaatacc cggttctttt gctgcgtaat cacattcagt
     6481 tcagcgttaa ttttttgacg gcgggcatct tccagcgaca acaagtcgag gccgccagac
     6541 ttccagttaa tttccgccgc tttaaagtat tgctggtatt gctgcgcact ggcttgttct
     6601 gattgtgccc gtcgcgttgc gctatccagg ttgaccagcg cctgctcaac ctctttgacg
     6661 gccttacgaa catcttgctt gtaggttgcg agcaaactgt caaaattggc acgtgcctct
     6721 gccaggtcag cgcgcaaggt cccgccatcg aagatgggca atttgagcga agggccataa
     6781 gaccaggagt ccgtgcgggt caccgcgcca ttaaagttgg tacgacggta accaatagag
     6841 cccgtcaggg tcaggctggg atacatctgc gctttggaca caccaatatt ggcgctagct
     6901 tcggccagat tacgttcatc cacggcaaga tcggggcgcc gtgtgagcaa gtttgccggc
     6961 atggcattca catcaaacat ggcaggctct ggcataccgt gccctttagc cagcaggttg
     7021 cgtagctcag gctcaggcag gttggttaac tccaccagcg ccttcaccag gccatcacac
     7081 tctgcctgct ggccatccag tgtcgactca ctggcacgag tggcagcctc tgccagtgcg
     7141 agatcagacg gggcagagaa cccggcattg gcggatatgc ctgtcaaacg tgcggtttca
     7201 ctacgcgagt cgtacgcctg ctttaaggcc tgcacggata cctggcaagc acggtagttg
     7261 acgtagttat tggccacctc agctgccaaa ctcaacctgg catcatgcca ggcgatcttc
     7321 ttggcctcta gctgcgcctt ggccgcctgt ttggccgctt tcaccttgcc aaacaagtcg
     7381 acctcccagc tcgcatccaa tgataccgag tcaatttcta tcgtactgga tggaaaagcg
     7441 ccaccacctc cccccttact ttttgtcata ctgccagtac tgctcaaatc tggaatgcct
     7501 tgtgcacggg ccgaaagcac attggcacgt gccacacgga tattcgccaa cgcaatctcc
     7561 agcgaaggac tctctttttg cgactcggca agcagttggt ccagtactgg gtcgttaaat
     7621 tgaccccacc agtcttttaa tgaagtcaca cttccaccat gtggcaaagg cgcttgccac
     7681 tgctcggcag tggctggttt ggggcggata tagtcatcaa tcactgagca acctgtcagt
     7741 gacaggcata acagcaaccc gcccaacccc atctttcccg actttaaaac accaggcctc
     7801 atttatttct catcccttgt aatccgttca agaacaattg caccacaaat gcaatgtgcc
     7861 cttctgcatc ccaatccggc tccaatatcc caaacagggc tttgttacgc ggacctgcga
     7921 tgaggagctc gcaatatgac tctgccgcct gcataaaatt ctgtacgggc aactcac
//
LOCUS       DATMSK010000163        11667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_152656,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000163 DATMSK010000000
VERSION     DATMSK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152656"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2151
                     /locus_tag="VNZ84_05465"
     CDS             <1..2151
                     /locus_tag="VNZ84_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HWU66438.1"
                     /translation="DEGVEVLGVTSYGDLTSFAQQQSRASAFILSIDDEEIVEEKPEA
                     IEQLRNFVAEIRYRNEEIPIFLHGETRTSRHIPNDVLRELHGFIHMNEDTPEFVARLI
                     IREAKAYLDSLPPPFFKALTHYAADGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENM
                     LRADVCNAVDELGQLLDHTGPVAASERNAARIYNCDHLYFVTNGTSTSNKIVWNSTVA
                     PGDIVVVDRNCHKSVLHSIIMTGAIPVFLMPTRNHFGIIGPIPKSEFAWENIQKKIER
                     NPFATDKNAKPRVLTITQSTYDGVLYNVEEIKEMLDGKIDTLHFDEAWLPHATFHDFY
                     GDYHAIGADRPRCKESMVFSTQSTHKLLAGLSQASQILVQDAQDNHLDRDLFNEAYLM
                     HTSTSPQYSIIASCDVAAAMMEAPGGTALVEESLKEALDFRRAMRKVDEEWGTDWWFK
                     VWGPNDLSEDGLEERDAWMLKANERWHGFGNLAEGFNMLDPIKATIITPGLDVDGEFS
                     EEFGIPAAIVTKYLAEHGVIVEKTGLYSFFIMFTIGITKGRWNTMVAALQQFKDDYDK
                     NQPLWKVLPEFVQKHPRYERIGLKDLCTQIHEVYKANDVARLTTEMYLSDMVPAMKPT
                     DAFSKMAHRKIERVAIDELEGRVTAVLLTPYPPGIPLLIPGERFNKVIVNYLKFAREF
                     NEKFPGFETDNHGLVKQIVDGKAVYYVDCVKQED"
     gene            complement(2867..3349)
                     /locus_tag="VNZ84_05470"
     CDS             complement(2867..3349)
                     /locus_tag="VNZ84_05470"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HWU66439.1"
                     /translation="MQALSLIVAHADNRVIGHNNQLPWHLPEDLKRFKQLTMGHHIIM
                     GRKTYESLGRLLPGRTTVIVTRDTEYAVDGAKVVHSLPEALTACAGDEEPFLIGGAEM
                     YAQGLAYVTRLYITRVYAHVGGDAFFPEIEMHDWELVSEEGHASVNGLHYSDMVYQRR
                     "
     gene            complement(3361..3900)
                     /locus_tag="VNZ84_05475"
     CDS             complement(3361..3900)
                     /locus_tag="VNZ84_05475"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWU66440.1"
                     /translation="MLIKLIMYGLLLWLAIWGYRQYQQPSTRVSVGMRAPEFKLPDAQ
                     GNMRSLSDWQGKWLVLYFYPKDDTPGCTREACQFRDDIQQIHALGAEVVGISVDTVAS
                     HAEFAQKHRLSFPILADSNGQVADAYGALFNLVVFQAAKRMTFIIDPQGVVAQTYSHV
                     NPDGHSQAIIDDLKRLSAF"
     gene            4032..5858
                     /locus_tag="VNZ84_05480"
     CDS             4032..5858
                     /locus_tag="VNZ84_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWU66441.1"
                     /translation="MNNEAIAKLLKLIAADPQKWEAYQKLAGLMLQNQEFHAAEQYLL
                     QAISLQPNTLELRLQLANLHMRTQQFKAAHECYKQAIALDAKQAHIYYLDARALKNLG
                     RTQEAILRLRTVTKLNPQHTEAYSECVDLMLQVQQVQQAAALLRQAIKAHPEHANFHY
                     RLGVLCQQLHATDEALECMNQAISIQPDFAEAYHQRGLMLQLQGKPQEALSSFHHAIA
                     HEPNLPVPYNNRGVILCQLGQYAEAINDFRQALKLNPGYASAYCNLGLALYENGNSDE
                     AYQCLGTALEYAPQHAQARYYLSMLKLAKGEYEDGWPMYQARWQTLPDKKRLDLQLLW
                     HGHESLVGKTILVQAEMTLSDTLQFCRYIPLIKAFKPNQIIVAVPETLYDLLNECWAQ
                     DAAVLVIRQDGRPLPHFDMHCPMLSLPTAFKTLVGTIPSSLPYIKIADRHQQPWQELL
                     GPARRLRIGLNWCGDAGNKYDKLRSIPLYLMAPLLKMEVEWHSLQKEFRREDHIMLKR
                     FPMLECHHPALMDFTQTAGLIMQMDLVISVDTAVAYLAAALGKTVWLLLPHQAHFRWL
                     QNREDSPWYPGMRLFRQEADETWENVIDRVHQAITDRLSQRD"
     gene            complement(5882..6712)
                     /locus_tag="VNZ84_05485"
     CDS             complement(5882..6712)
                     /locus_tag="VNZ84_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWU66442.1"
                     /translation="MVTLTEARDNRTVHHWVAFLKQADIPVLRQTARELAALQEREEE
                     TGARDITRVVINDPLMMFKVLTFANNNRSRHQLQDLVQVEQAIIMMGTSTFFAQIPTV
                     PHVEEVLNQHVPALLSLLKLMIRAHRAGYFAAEFASHLMDLHAEELRVAASLYDFAEM
                     LMWCFNPDAMNEISQRQAADKTLRSRVVQQEVLGFRIVELQAELVNVFNLPSLLTDLM
                     DEKRSHLPRVRNVQLSVNLARHSADGWDNAALPDDYKDISQLLHVDVERVMHIVGVPR
                     "
     gene            6922..7740
                     /gene="map"
                     /locus_tag="VNZ84_05490"
     CDS             6922..7740
                     /gene="map"
                     /locus_tag="VNZ84_05490"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWU66443.1"
                     /translation="MALKVVKHMAIILHTPEEVEKMRVAGRLASEVLDYITPFVVAGV
                     TTNKLDQLCHDYIVNVQQAIPAPLNYAPDGHTPYPKSICTSINQQICHGVPSEKELKN
                     GDIVNIDITVIKDGYHGDTSRMFMVGTVTPQAKRLCELTYQAMWRGIEKVKPGNTLGD
                     IGYAIQSFAEKNGYSIVREFCGHGIGKKFHCEPQVLHYGKPGAGVTLKPGMIFTIEPM
                     INAGRREIKFMSDGWTVVTKDRSLSAQWEHTVLVTEDGFEIMTVSAGTPPRPSL"
     gene            7752..10328
                     /locus_tag="VNZ84_05495"
     CDS             7752..10328
                     /locus_tag="VNZ84_05495"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HWU66444.1"
                     /translation="MTVIAKEQLKHWRESLQEARQKIADDYLIKPQAKTLLQQHTRIL
                     DQLLRDIWHSYALPLNVNLIAVGGYGRGDIFPHSDIDLLILMPENSPPPLQTDVESLI
                     GTLWDIGLNIGHSVRTLRECLDEAKLDVTIMTNLLEARWLTGDKSSFKTLTQSLHAEL
                     DSARFFAEKLREQQIRHAKFHDTAYNLEPNIKESPGGLRDLHMILWVAQSQNLGHDWN
                     DLVHNGLLTRGQASQLKRHERLLQNLRIRLHLLAKRREDRLVFDFQNALAEQMGYQTT
                     SQKRASEQLMQGFYRSAKAIIIENEVLLKLMHARVNPATEPATAIDEDFTLSQGLLSA
                     QSADLFQRKPEAILRCFQLLQQHPAITGFSAPLVRALQAAQSLINTHFRQNAAYKALF
                     LQILQSREGVHRSLRRMNRYGILGRYIPVFGRVIAQMQHDLFHVYTVDEHTLNVLRNL
                     RRFAKPDLRHEFPLCSQLFNEFSQPHLLYLAAIFHDIAKGRGGDHSKLGTIDARRFCL
                     KLGLPKADCELVAWLVEAHLKLSSTAQKSDLSDPDVIEAFAQLVGSEYRLTALYLLTV
                     ADIRGTSPNVWNAWKAKLLESLFLQTRRVLQQSLDTEAQLNLRKQEVLQKFKSFNLKE
                     ASVQPLWQAFGPGYFNRFDSDEIAWQSRLLIPHQRTEKPIVRARLSPNGDGIQVMIYS
                     RDQKEIFARICHFFDSMQYNIVQAKIYTTAHGYALDNFIVLEPETQQISYNGLLKHIE
                     QGLTDQLLSDKAIPTPIRGRVSRQVKHMPISTQIFLRPVINQQEPTTLFQQLDVIAND
                     RPGLLASMALLFLNHGIELHNAKINTLGNRVEDSFLISACHGQAINESQVTALKQAFS
                     EL"
     gene            complement(10351..10623)
                     /locus_tag="VNZ84_05500"
     CDS             complement(10351..10623)
                     /locus_tag="VNZ84_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU66445.1"
                     /translation="MSAIIVEKPTPARLNELGVKQWPTWSKEVSTFPWQYSSTEIAYI
                     LEGEVTVTPNGGTPVSFSAGDLVTFATGLDCVWEVKKPLRKYYHFE"
     gene            10815..>11667
                     /locus_tag="VNZ84_05505"
     CDS             10815..>11667
                     /locus_tag="VNZ84_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66446.1"
                     /translation="MFTYPQLTASEQASLPKLIAQPTTIPNKFEDSTHQLFCCQTTDG
                     EMVLKVCNEATIRKSHFWSGANHLFAADFPNSLGNIHSTHCFLEKNGALAVPEFVSAN
                     AHRYVLTRYLHGQDIEAEYITDEWVIALAEHIAKLHQCTYIGWGQLHKPEFSAAEWAG
                     RLHDTLAFLSKQHDTGIADPLVQEVLTHAKNIQESDFVPVMLDLRWDQFRLIHAGSHH
                     LALIDLDAFVIAPRALDLVLLEYVLTPAQLALFKQHYTLIHNWPDVTVQKPCYQLLLF
                     LMNILGET"
BASE COUNT         3051 a         3055 c         2756 g         2805 t
ORIGIN      
        1 gatgagggcg tcgaagtgct gggcgtgacc agctatggtg acttgacttc gttcgcacaa
       61 cagcaaagcc gcgcctcggc atttatcctg tctatcgacg atgaggaaat cgtagaggaa
      121 aaaccggaag ccattgagca attacgcaat ttcgtagctg aaattcgtta ccgtaatgag
      181 gaaattccta tcttcctgca cggtgaaacg cgcaccagcc gccatatccc taacgatgtg
      241 ttgcgtgagt tgcatggttt tatccacatg aatgaagata caccagaatt tgtggcacgc
      301 ctgattatcc gtgaagccaa agcttatctg gacagcctgc caccgccatt cttcaaggca
      361 ctgacacact atgcagcgga tggctcttac tcctggcact gccctggcca ctccggcggc
      421 gtcgcattcc tgaaatcacc cgtcggccag atgtttcacc agttttttgg tgaaaacatg
      481 ttgcgtgcag acgtttgtaa cgctgtagat gaattaggtc agttactaga ccataccggt
      541 ccggtggcgg cttccgagcg taacgctgcg cgtatttaca actgcgacca tttgtacttt
      601 gtcaccaacg gcacctcgac atcgaacaag attgtctgga attctactgt ggcgccaggt
      661 gacatcgtgg tcgttgaccg taactgccat aagtcagtcc tacactcgat tatcatgacc
      721 ggtgcgatcc ccgtattcct gatgccgacc cgcaatcact ttggcattat cggcccgatc
      781 cctaaaagtg aatttgcctg ggaaaacatc cagaaaaaaa tcgagcgcaa cccgtttgcg
      841 actgacaaaa atgcaaaacc acgtgtatta accattacac aatcgaccta tgacggcgtg
      901 ttgtacaacg tggaagaaat caaggaaatg ctggatggca agatcgacac cttgcacttc
      961 gacgaagcct ggttaccaca tgcgactttc catgactttt acggtgacta ccacgcaatt
     1021 ggcgcggatc gtccacgctg taaagaatcc atggtgttct ctacccaatc tacgcataaa
     1081 ctgctggcgg gcttaagcca ggcctcacag atcctggtgc aggacgcgca agataaccac
     1141 ctggatagag acttgtttaa cgaagcctac ctgatgcaca cctctaccag cccgcaatac
     1201 tcgattattg ccagctgcga cgtggctgcg gccatgatgg aagcaccagg tggtacagca
     1261 ctggtagaag aatcgcttaa agaagcgctg gatttccgcc gtgcgatgcg caaagtggac
     1321 gaagaatggg gcaccgactg gtggtttaaa gtctgggggc cgaatgactt gtctgaagat
     1381 ggcctggaag aacgcgatgc ctggatgcta aaagccaacg agcgctggca cggtttcggc
     1441 aacctggccg aaggcttcaa catgctggac ccaattaaag cgaccatcat cacgcctggc
     1501 ctggatgtag atggtgaatt ctctgaagaa ttcggtattc ctgcggccat cgtgaccaag
     1561 tacctggccg aacacggtgt gatcgttgag aaaaccggtc tctactcatt ctttatcatg
     1621 ttcaccatcg gcattaccaa aggtcgctgg aacactatgg ttgcggcctt gcagcaattt
     1681 aaagatgatt acgacaaaaa ccagccacta tggaaagtgt tgcctgagtt cgtgcagaaa
     1741 catccacgct acgagcgcat tggcctgaag gatttatgta cacaaatcca tgaagtgtac
     1801 aaagccaacg acgttgcacg tttaaccacg gaaatgtacc tgtccgacat ggtgccggcg
     1861 atgaaaccaa ctgatgcttt ctctaaaatg gcacaccgca aaattgaacg cgtggccatt
     1921 gatgagctgg aaggtcgcgt gactgcagtg ctgttaacgc cctacccacc gggcatccca
     1981 ctgctgattc caggggaacg ttttaacaag gtgattgtga actacctcaa gtttgcacgt
     2041 gagtttaatg agaaattccc gggtttcgag acggataatc atgggttggt gaagcaaatt
     2101 gtggatggta aagcggttta ttacgtggat tgcgtaaagc aagaggatta gtttctagta
     2161 atgcgatgag ggaaacgcca aagcgtttcc ctttttgctt ttaaggctat tgctattgca
     2221 tgcatttttg cttttcgcct ctaggcgagt tactttctgt tgcttgcccc acagaaagta
     2281 accaaagagg agggcaccca gccatcacgg cctacggctg ccttgcgttg ctcaacaaaa
     2341 taagtcggtc gcgaaactca accttgcagc ccacaaacta cgtgagctgc tgcgggattc
     2401 aaacagccgc tccctctact cttattttgt ctccgctact cagcgtgatg ggatgggatt
     2461 tgtctcgcca aactcacagc gtagaaatat ggacggttta aaactctaat tctgttgttg
     2521 aaacaaatga cggttgcgct cgtatggtgt tcggggtccc catctgccgc acgctttgat
     2581 taaagcagtg caacaccggt ttggcgggag ttctcggcca tcggctgtcc gagttccgca
     2641 acaagccacg ttttgtgtgg cttgtcaggg cgagtttcga tggacgcccg gcaatccgag
     2701 tagcacagga caaaagcgga agctggggtg cactttcttt tggttacttg ttctttgtgc
     2761 aagcaaagaa aagtaactcg ccgaaaaggc gaaaagcaat gcatcaacac tcccattcaa
     2821 agaatgactt ttttaaaact atctctatct ggtcaaatca tctctatcag cgccgctgat
     2881 aaaccatatc actataatgc aagccattca ccgaggcatg cccttcctca ctcaccaatt
     2941 cccagtcatg catctcaatt tccggaaaaa atgcatcgcc gcccacatgc gcgtagactc
     3001 gcgtgatgta taaccgcgtg acataagcca gaccctgcgc gtacatctct gccccaccga
     3061 tcaagaacgg ctcttcatca cccgcacacg cagtcagtgc ctcaggcagt gaatgcacta
     3121 ccttggcacc gtccacggca tattcagtat cgcgcgtgac aatcacggtc gtgcggcccg
     3181 gcaataaccg tcccagtgac tcataggttt tgcgtcccat gatgatatgg tgacccatgg
     3241 tcagttgctt gaaacgtttg aggtcttctg gcagatgcca tggcaattga ttgttatggc
     3301 cgatcacacg gttatctgca tgcgcgacga tcaaggataa ggcttgcatg gctatattct
     3361 ctaaaaagca gacaggcgtt tcaggtcatc aataatggcc tggctatggc catctggatt
     3421 cacgtgactg taggtttgtg caacgacacc ttgtggatca atgataaaag tcatgcgttt
     3481 ggctgcctga aaaaccacaa gattaaataa cgcaccataa gcatccgcca cttggccatt
     3541 actatcagcc aaaatgggga atgagaggcg gtgcttttgt gcaaattcgg catggctggc
     3601 tactgtatcc acactgatcc ctaccacttc agcacccagt gcatgaattt gctgtatatc
     3661 atcccggaac tggcaggctt cacgggtaca ccctggcgta tcatccttgg gataaaaata
     3721 cagtaccagc catttgcctt gccagtctga caggctacgc atattgcctt gagcatctgg
     3781 caatttgaat tctggcgccc gcatgccgac actgacacgt gtagatggct gctggtactg
     3841 gcggtatccc cagatggcca gccataacaa tagtccgtac atgatcagtt tgatcagcat
     3901 gcaatttttc ctctctacaa cagatttatg acattcaggt cgttgacgct tacagctgct
     3961 tattgtacct taatccgcca tctgtcattt taaggtcatc cgcaatcgtt tgaaggtttg
     4021 cagtcactcc catgaataat gaagccattg caaagctgtt aaaactgatt gccgcggatc
     4081 cacaaaagtg ggaggcctac cagaaactgg ccggcctgat gctgcaaaac caggagtttc
     4141 atgcggctga gcagtacctg ctgcaagcca tcagtttgca gccgaatacg ctggaactgc
     4201 gcctgcaatt ggccaatctg catatgcgta cacagcagtt taaagcggca catgagtgct
     4261 ataaacaagc cattgcactg gatgccaagc aagcccacat ttattacctg gatgcccgcg
     4321 cactcaagaa tctcgggcgt acccaggaag ccattttacg tttacgcacc gtcaccaaac
     4381 ttaatccgca gcataccgag gcttatagtg aatgtgtcga tttaatgtta caggtccagc
     4441 aagtacagca ggcagctgct ctgctcaggc aggcgattaa ggcacatccg gagcatgcca
     4501 attttcacta ccgccttggc gtactctgcc agcaactgca tgcaactgat gaagcactgg
     4561 agtgcatgaa tcaggctatc agcattcaac ctgactttgc ggaggcctac catcaacgcg
     4621 gactgatgtt gcagttgcaa ggcaagccgc aagaggcgtt gagcagcttc catcatgcca
     4681 ttgcccatga acccaacctg cccgtgccct ataacaatcg tggggtcatt ttgtgccaac
     4741 ttggccaata tgcagaggct atcaatgatt tccgccaggc gctgaaactg aatccaggct
     4801 atgcctcggc ttactgcaac ctgggacttg ccctgtatga aaatggcaat agcgatgaag
     4861 cttatcaatg tttggggacc gcactggaat atgcaccaca gcatgcgcaa gcccgttact
     4921 acttgagcat gctcaaactc gccaagggtg agtatgagga tggctggccc atgtatcagg
     4981 ctcgctggca aacactgcct gataaaaagc gcctggattt gcaattgtta tggcacggcc
     5041 atgagtcatt agtcggtaaa acgattctgg tccaggctga aatgacgctc agcgacacat
     5101 tacaattttg ccgctacatt ccgctcatca aggcttttaa accgaatcag atcattgtcg
     5161 cggtacccga gactttgtat gatttgctga atgaatgctg ggcacaagac gcagcggtgt
     5221 tagtcattcg ccaggatggc cgccccctgc ctcactttga tatgcactgc ccgatgctga
     5281 gtttgcctac agcatttaag accttggtcg gtacaatccc ctccagcctg ccctatataa
     5341 aaatcgctga ccggcaccaa caaccgtggc aggaattact cggtccggca cgccggttgc
     5401 ggataggcct caattggtgc ggggatgctg gcaacaaata tgacaagctg cgcagtattc
     5461 cactgtacct catggcaccg ctgctcaaaa tggaggtcga atggcatagc ttgcaaaaag
     5521 aattccggcg tgaagaccat atcatgctta aacgcttccc catgctggaa tgccatcatc
     5581 cggcattgat ggattttacg caaactgccg gattgatcat gcaaatggat ctagtgattt
     5641 cggtcgatac cgctgttgcc tacctggctg cagcactggg aaaaaccgtg tggctattgt
     5701 taccgcatca ggcgcatttc cgctggttgc aaaacaggga agacagccct tggtatccag
     5761 gcatgcgctt attcaggcaa gaagcggatg aaacttggga gaatgtgatt gaccgcgtgc
     5821 accaggccat taccgacagg ctgtcacaac gcgactagtc tgttgcaaac cagggactaa
     5881 actagcgagg aacccctaca atatgcatta cacgctccac atcgacatgc agtaactgtg
     5941 agatgtcttt gtaatcatcc ggcaatgcag cattatccca accatctgct gaatgcctgg
     6001 ccagattcac agataactgt acgttacgga cacgcggcaa atgcgagcgc ttttcatcca
     6061 tcaggtcggt cagcaacgag ggcaaattaa acacattcac gagttcggct tgcaactcca
     6121 cgattctgaa gccaagtacc tcttgttgca caacgcggct acgtaaggtt ttatccgccg
     6181 cctggcgctg actgatttca ttcatggcat ccgggttgaa gcaccacatc agcatttcag
     6241 caaaatcgta taatgacgcg gcaacacgca actcctctgc atgcagatcc atcaaatgtg
     6301 atgcaaactc agcggcaaaa taacctgccc tgtgtgcacg tatcatcagt ttaagcaggc
     6361 tcaacaaggc ggggacatgc tggttaagca cctcttctac atgtggcacg gttggaatct
     6421 gagcaaaaaa agtactggtc cccatcatga taatggcttg ctctacctgt accaggtcct
     6481 gcaactggtg ccgactgcgg ttgttattgg caaaggtgag gaccttgaac atcatcagcg
     6541 gatcattaat cactacgcgg gtaatatccc tggcgccagt ttcttcttcg cgctcctgca
     6601 acgcagccaa ctccctcgcc gtttgacgca aaacagggat atccgcctgt tttaaaaagg
     6661 caacccagtg atgtacagtc cggttatctc ttgcttcggt gagtgtcacc atatttccgc
     6721 tccagctcga ttcatgcgcc cgagtataaa acatcaaggc ttgatctcat gccgcaatca
     6781 atcacagaat tgtttgcctg tttgttttta tggcatatct gctttaacgt cacaaaaggc
     6841 gtgcttcttt aggcatggct gataaatgcg tatgttagaa tggcatttta gttattgctt
     6901 tccaagccag attcaaacga tctggcccta aaggtcgtca agcatatggc aattattctg
     6961 cacacccccg aagaagtcga aaaaatgcgc gtcgccggcc gtctggcatc cgaggtgctg
     7021 gattacatta cgccgttcgt tgttgctggc gttacaacca ataaactaga ccagttatgc
     7081 cacgactaca tcgtgaatgt gcaacaggcg attcctgcac cattaaacta tgcacctgat
     7141 ggccacacac catatcccaa atccatctgc acctctatca atcagcaaat ctgtcacggc
     7201 gtgcctagcg agaaagagct taagaacggc gatatcgtta acatagacat cactgtgatc
     7261 aaagatggtt accatggcga cacttcgcgc atgtttatgg ttggaaccgt aaccccgcaa
     7321 gcaaaacgcc tgtgcgaact gacttaccag gccatgtggc gcggcattga gaaagtcaaa
     7381 ccaggcaaca cactgggtga catcggttat gccatacaat cctttgctga aaagaatggt
     7441 tacagcattg tgcgcgaatt ttgtggtcac ggcattggca agaaatttca ttgcgagccg
     7501 caagtactgc attacggcaa gcctggtgca ggtgtcacgc tcaaacccgg catgattttc
     7561 accatagagc ccatgatcaa tgctggacgt cgcgagatca aattcatgtc agatggctgg
     7621 acggtcgtga ccaaagaccg cagcctgtcc gcgcaatggg agcataccgt actggtgact
     7681 gaagacggtt tcgaaatcat gacggtctct gccggtaccc caccgcgccc atccttgtaa
     7741 gtgacgcagc catgacggtg attgccaaag aacagctcaa gcactggcga gagtcgttac
     7801 aagaggctcg ccagaaaatt gcagatgatt acctgatcaa gcctcaggca aaaacactgc
     7861 tgcaacaaca cacccggatt cttgatcagc tcctccggga catctggcat agctacgccc
     7921 ttcctctcaa tgttaacttg attgctgtcg gtggttatgg gcgtggcgat atattcccgc
     7981 attcagatat cgacctgctg atcctgatgc cggaaaacag cccaccgccc ttgcaaacgg
     8041 atgttgagtc cctgattggc acgctatggg atattggcct caacattggc catagtgtac
     8101 gcaccttgcg cgagtgcctg gatgaggcca agctggacgt caccatcatg accaacttgc
     8161 tggaagcacg ctggctgact ggtgataaat ccagctttaa aacactgacg caatcattac
     8221 acgcagaact cgacagcgcg cgtttttttg ccgagaaact gcgtgagcaa caaattcgcc
     8281 acgctaaatt ccacgatacg gcttataacc ttgagccgaa catcaaggaa agccccggcg
     8341 ggctgcgtga tttgcatatg attttatggg ttgcgcaaag ccaaaacctg gggcatgact
     8401 ggaacgacct ggtgcacaat ggcttgctga cacgcggcca ggccagccag ctcaaacgac
     8461 acgaacgcct gctgcaaaac ctgcgtatcc gcctgcactt gctggccaag cgccgtgaag
     8521 accgattggt atttgacttt caaaacgccc tggctgaaca aatgggttat caaaccacct
     8581 cgcaaaaacg tgccagtgag caattgatgc aaggttttta ccgtagtgcc aaagccatca
     8641 ttattgaaaa tgaagtgctg ttaaaactca tgcatgcccg tgtaaatccg gctacggaac
     8701 cagccacagc aatagatgaa gattttacgc tgtcacaagg cttgctcagt gcacaaagcg
     8761 ctgacctgtt ccaacgcaaa cctgaagcca tcttgcgctg cttccagctt ttgcagcaac
     8821 atcctgccat cactggcttt agcgccccgc tagtccgggc cctacaagca gcccagtccc
     8881 tgattaatac tcactttagg caaaatgcgg cctataaagc cttattcctg caaattctgc
     8941 aaagccgtga aggggtgcac cgcagcctgc gccgtatgaa ccgttatggc attttagggc
     9001 gctatattcc tgtatttgga cgtgtcatcg cgcaaatgca acatgacctg ttccatgtct
     9061 acacggtaga tgaacacacc ctgaatgtct tacgcaactt gcgccgtttt gccaagccgg
     9121 acttacgcca tgagtttccg ttatgcagcc agctattcaa tgaattcagc caaccacacc
     9181 tgctttacct ggccgctatt tttcacgata ttgccaaagg ccgtggcggt gaccactcaa
     9241 aattaggcac catagatgcc aggcgctttt gcctgaagtt gggcttaccg aaagctgatt
     9301 gcgaactggt ggcctggctg gtcgaagcac acttaaaact ctcaagtacc gcacaaaaaa
     9361 gcgacctgtc agacccggat gtgattgaag cgttcgcgca attggtcggc tctgagtacc
     9421 gcctgacagc cttatacctg ttaacggttg ccgatatcag gggcaccagc cccaatgtct
     9481 ggaacgcctg gaaagccaaa ctactggaga gcctgttcct gcaaacacgc cgtgtactgc
     9541 aacaatcgct ggacactgaa gcgcaactca atttgcgtaa acaggaagta ttacaaaaat
     9601 tcaagagctt caatctgaaa gaggcttccg tacaacccct gtggcaagca tttggacctg
     9661 gttatttcaa ccgtttcgac agcgatgaga ttgcctggca aagccgtttg ctgatcccgc
     9721 atcaacgaac agaaaaaccc attgtacggg cgcgtctcag tcccaatggc gatggcattc
     9781 aggtcatgat ctacagccgt gaccagaaag aaatttttgc ccgcatctgc catttctttg
     9841 acagcatgca atacaacatt gtgcaggcca aaatctacac gactgctcac ggctatgcgc
     9901 tggacaattt tattgtcctg gagcctgaaa cacagcaaat cagctataac ggcttgctca
     9961 agcatattga gcaaggcctg acggatcagc tactcagtga caaggctatt ccaacaccga
    10021 tacgcgggcg tgtcagccgg caagtcaaac atatgccgat ttcaacccag attttcttgc
    10081 gacccgtcat caaccagcaa gaaccaacca cgctatttca acaactggac gtgattgcca
    10141 acgataggcc tgggttgctg gccagcatgg cgctcttgtt tttgaaccat ggcattgaat
    10201 tacacaatgc caagatcaat acgctaggca accgcgtcga agatagtttc ctgatttcag
    10261 cctgtcacgg ccaagcgatc aatgaaagcc aagtcactgc gctgaagcag gcttttagcg
    10321 agttataacc agcctgggtt aagagtttcg ctactcaaaa tgataatact tgcgtagcgg
    10381 ctttttcact tcccagacac aatccaggcc cgtcgcaaac gtcaccaaat cacctgcaga
    10441 aaagcttact ggtgttcctc cgttgggcgt cacagtcacc tcgccttcca gaatgtaggc
    10501 aatttctgta ctgctgtact gccaagggaa ggtagatact tctttggacc aggtaggcca
    10561 ttgcttcaca cctagctcgt tcagacgcgc tggcgtaggc ttctcaacaa taatcgctga
    10621 catatcgctc tccattttta tagtgaaaag ctattctaat cacctgtcag tgccaaatac
    10681 aaccttccat gaagtaagta tcttggaaga acccatgcca cacgacattg aatacggaac
    10741 cagaaaaacc aagcaagtga ggttttctta aagatgattt caacgatcgg atgacttaaa
    10801 atataggcct gaccatgttt acttatccgc aactcaccgc atccgaacaa gccagtttac
    10861 ccaaactgat tgctcaaccc actactatcc cgaataagtt tgaagatagt acgcatcagc
    10921 ttttttgttg ccaaacaacg gatggtgaaa tggtgctgaa agtatgtaat gaagcgacta
    10981 taagaaaatc tcacttctgg tcaggcgcaa accatttatt tgctgctgac ttcccaaaca
    11041 gtctgggcaa cattcattca acgcattgtt ttttggagaa aaacggtgcg cttgccgtgc
    11101 cagagtttgt ctcagccaat gcccaccgat acgtactcac gcggtacctg catggtcaag
    11161 atatagaagc ggaatatatc accgatgaat gggtaatcgc gctggccgaa catattgcca
    11221 aattgcatca atgcacctat atcggctggg gacaactaca caaaccagaa ttctctgcag
    11281 cagaatgggc tggccgttta catgacacgc ttgcttttct gtccaagcag cacgatacgg
    11341 gcattgctga tccgttagtg caggaagtgt taacgcacgc aaaaaatatc caggaaagcg
    11401 actttgtacc cgtgatgctg gatttgcgct gggatcaatt ccgcctcatt cacgctggca
    11461 gccatcatct tgcactgatt gatcttgatg cgtttgtgat tgcaccgcgt gcgctagact
    11521 tggtattact ggaatacgtg ttaacacccg cgcaattggc tttatttaag cagcactata
    11581 cgctaataca caattggcct gacgttactg tgcagaaacc ctgttaccag ttactgctgt
    11641 ttttgatgaa tatactgggt gaaacta
//
LOCUS       DATMSK010000164         7021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_176041,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000164 DATMSK010000000
VERSION     DATMSK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176041"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VNZ84_05510"
     CDS             complement(<1..432)
                     /locus_tag="VNZ84_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HWU66447.1"
                     /translation="MADNNEIISTLNDLIEVSKDGEEGFRSSAENVDDPQLKTFFLRR
                     SKEVATSVQELQELVRSLGGKPADSSSLSGTLHRRWIDLKTALTSNDTVAVLNETERG
                     EDVALATYRKAAEKELPPHIRFVVVRQLEGAKRNHDEVKRLQ"
     gene            complement(556..1689)
                     /locus_tag="VNZ84_05515"
     CDS             complement(556..1689)
                     /locus_tag="VNZ84_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWU66448.1"
                     /translation="MEQWLHTNVWLGIPLYEWLYAALVSSLLYLGFYQGWKIIISRLS
                     KIAERTSTKLDDISIEVLQSTQQLTLLLLATLIGLHVLELPDKWATRLDHVLFITIGI
                     QAAIWLNKGVGIWAREHLAAIDGPAPAANPVMTTVLSWILRVFIWSVALLTVLDNVGV
                     NITAFVASLGVGGVAVALAVQNILSDLFASLAIGLDKPFEVGDFVVFGDIAGVIERVG
                     LKTTRIRSISGEQVICSNTELLKNTIHNYKRMEERRVVFTFGINYATPPDTIADIPGI
                     VRKAVEAFKNTRFDRAHFKAFGASSLDFEIVYFITSTDFNVYMDIQQGINLYLMKAFQ
                     AHGVDFAFPTMTLNMPDLIRDNTANQASTPSSPTREKGQAAKH"
     gene            1924..2529
                     /locus_tag="VNZ84_05520"
     CDS             1924..2529
                     /locus_tag="VNZ84_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HWU66449.1"
                     /translation="MNPIRLFYFVSPKNHHAKLLTAEKPSVSQLLFFYVLPLSLIPCV
                     MMYFRMYESYPKLFFDNLPGNRMMMVSIELLFLQLLVVLTMAWITQNLAEMVNIKPNF
                     RDSLLLVALATTPLWMTSLFYLVPSLSFNVLVHGVAVLAAVLLVYRGVRYIFGIRERG
                     ALITLSMAIVCTAGLGFAVILIGSLISWEAIEQLQFAVKKM"
     gene            complement(2540..3196)
                     /locus_tag="VNZ84_05525"
     CDS             complement(2540..3196)
                     /locus_tag="VNZ84_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU66450.1"
                     /translation="MKNILLIYGHALSHSALRELLNEDPHLHLADEVANAQDALQLIK
                     TQQYDMVILDVSAPEKNGLETFCDLKQMAPDLPILIINGSESTDRMVHFLRLGCAGYL
                     PYSTDSAQVLAAMKTIARKQPYRLPEQQAMMNGKRHLLHERLSLRELQVFFKLIKGQP
                     VNAIAIELAIAPGSVSVFRSKILKKMDASNNAALIHYALAYQLTPPIGGASKPMSLSS
                     "
     gene            complement(3247..4200)
                     /locus_tag="VNZ84_05530"
     CDS             complement(3247..4200)
                     /locus_tag="VNZ84_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HWU66451.1"
                     /translation="MRGKFRYLDAQGQCLKCRQSLSRIAKLRIPPAWESVWICPHPQG
                     HLQATGRDAKGRKQYLYHAAWRLQRENKKYAHMVAFGQQLPIIRQQVDRDLKTSGMGK
                     QKVLAMMIRLLENTLIRIGNDAYARTNKSFGLTTLRNRHVAIENNDVEFHFRGKSGVE
                     HRVQLHDPCLARMMRQLRELPGQALFQYVDSEGLQHAIGSTDVNDYLRQLSGDDYTAK
                     DFRTWFGSLHTLVALSAFPPCHSEAEAKKNIVAAIRLAASRLGNTPAICRHSYVHPSI
                     IDSYLAVCGCKWMPLMQATNNDWPALSMAEQSMLELLQQRT"
     gene            complement(4277..5686)
                     /locus_tag="VNZ84_05535"
     CDS             complement(4277..5686)
                     /locus_tag="VNZ84_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HWU66452.1"
                     /translation="MPSKPPSQSSMRLIASAGILALSACSQPAKLPETAGFGPDPDLS
                     SYQTGLMPTIHVAEAVGWPEGKKPTSPPGTNVVAFASDLHHPRWLYELPNGDILVAET
                     NHHEKSDGFELKSWIASKFMKDGGAKAESMNRILLLRDNDGDGVADERHIFIQDLHSP
                     FGMALIGDTFYVANADALVKFPYQEGQTEITAAPIKVTSLPAERNHHWTKNILPKQDG
                     SKLYVTIGSNSNVGENGMEIEKERAAIWEVDPDSGQHSIYASGLRNPNGMAWEPDSGA
                     LWVVVNERDELGSDLVPDYMTSVTQGAFYGWPYSYYGQHVDDRIQPQKPELVAKAVKP
                     DYALGSHTASLGLTPAKGNQLPDQFKNGMFVGQHGSWNRRPYSGYKVIFVPFEAGKPA
                     GQPIDVLTEFLNTDGQAQGRPVGVLVDKKGHLLVADDVGNVIWNVKGNGNSRKTAHQE
                     RTQEIVSEAEQTGIEISEN"
     gene            complement(5712..7001)
                     /locus_tag="VNZ84_05540"
     CDS             complement(5712..7001)
                     /locus_tag="VNZ84_05540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HWU66453.1"
                     /translation="MQFETPLLDQPQPWQWDENVQTAANGETSQAGFILNRLFQDYPG
                     AFSVRLWNGNLLNIGEGDPAFTFCLQQASVLRNMVWFCDPKCLAEAYFSGQIQILGDF
                     HAAMQLREHFESLSLPLHEKLGLIFRALTLATDFAQPEQTSLPQIDPTPDQLDTSSPV
                     ALSYDNVPEDFYQCWLDEHMLHSCAYFSGNTRDLAQAQRNQLDLICLKLHLQHGDSLL
                     DIGCGWGGLACWAAKHYGVFVHGITQNASQYAYATKQVKRLGLEHLVKIELGSYQDLP
                     ATGSYDKAACIGIADHIGEAHFPDYLAKVHAALKPGGLFLTHGITNEQLAGQQDASSA
                     FINRQLFPQGDLSTFPHFLQQMDAAKFDVFNVESLRRHYVMTLHQWLAGLEANHATVA
                     SLVGERTYRIWRLYMTASAIQFEQGVTGIHQVVATRR"
BASE COUNT         1581 a         1780 c         1819 g         1841 t
ORIGIN      
        1 ctgcaaccgt ttcacctcat catggttacg tttggcacct tcaagctgcc ttacgacgac
       61 aaaccggatg tgtggaggca actctttttc ggctgccttg cggtaagtgg ccagggcgac
      121 gtcttcaccg cgttcagttt cattgaggac ggcgacagtg tcgttgctag tcagcgctgt
      181 tttcaggtca atccagcgtc tatgcagggt gccgctcaag ctgctcgagt ctgcaggctt
      241 accgcctagc gagcgtacca gctcttgcaa ctcttgcacg ctggtagcta cctctttaga
      301 acgacgcaaa aagaatgttt tcagttgtgg atcatccacg ttctcggccg aagaacggaa
      361 tccttcttca ccgtctttgg atacttcaat gaggtcattg agggtagaaa taatttcgtt
      421 attgtctgcc atgttttcgt ctccaagcta tcaaagagta gttaagaaac tttagtatgc
      481 ctatgaacta tcagtggcta catcggagga atacttaaag cgatgtagga attactttat
      541 gtccacaggc attgctcaat gcttggccgc ctggcctttt tccctcgttg gcgaggacgg
      601 cgtggaggcc tggttagcag tattgtccct gataaggtca ggcatattga gtgtcatggt
      661 cgggaacgca aaatctacac catgcgcctg gaatgctttc atcaggtaca ggttaatgcc
      721 ctgttgaata tccatataga cgttaaagtc agtagaggtg atgaaataaa caatttcgaa
      781 atccagcgag ctggcgccga atgccttaaa gtgggcccgg tcaaaccgtg tatttttaaa
      841 cgcttccacg gctttgcgta cgatgcccgg gatgtctgca atggtgtcag gaggcgtggc
      901 gtaattaatg ccaaaggtaa acacaacgcg gcgttcctcc atgcgtttgt agttatggat
      961 cgtatttttc agcagctcgg tgttgctaca gatgacctgc tcgccactga tactccggat
     1021 gcgggtggtt ttcagcccca cgcgctcaat caccccggca atatcgccaa agactacaaa
     1081 gtcaccgacc tcaaaaggtt tatccaggcc aattgccaag gacgcaaaca ggtcacttaa
     1141 gatattttgc accgccaggg cgacagcgac gcccccaacc cccaaactgg ccacaaatgc
     1201 ggtgatgtta acgcccacat tatcaagcac ggttagcagg gccaccgacc agataaatac
     1261 ccgcaatatc caggacaaca ccgtggtcat gaccggatta gctgcagggg ccgggccatc
     1321 aatcgccgcc aggtgttcac gcgcccagat acccacgccc ttgttcagcc agatagctgc
     1381 ctggatgccg atggtgataa acaggacgtg atccagccgc gttgcccatt tgtccggtaa
     1441 ttccagcaca tgcaggccaa tcaatgtggc caggagcaat agcgtgagtt gttgggtgct
     1501 ttgcaacacc tctatcgaaa tatcatccag cttggtggag gtcctttcgg cgattttcga
     1561 caatctggaa atgatgatct tccagccttg atagaagcca aggtatagca gcgaagaaac
     1621 aagtgccgca tatagccatt cataaagggg gatgcctaac cagacattgg tgtgtaacca
     1681 ctgttccata gggggttcct tgctaagtca atgaatcaaa tgacatttta agtaagagac
     1741 ggtttacagg ctgtaggcgt gcactgattt agctgtcaga gtcgtccaca ttgcagttgc
     1801 attctcctac acttaaatca gccattgccg tgtagcgcct tgttttgacc tgtcttcata
     1861 atgggacgtg tctagtggcc gcttggttcg ccacgttatg cattaatgat gaaggagcac
     1921 gacatgaacc ctatccgctt gttttacttt gtttccccca aaaatcatca tgccaagttg
     1981 ctcaccgcag aaaagcctag tgtctcgcag ttgttgtttt tttatgtatt accgctttca
     2041 ttgattccgt gcgtcatgat gtatttccgc atgtatgaga gctaccccaa gctgtttttt
     2101 gataacctgc ccggcaaccg gatgatgatg gtgagcattg agctgctgtt cctgcagcta
     2161 ctggtggtgt tgaccatggc ctggattacg caaaacctgg ccgagatggt gaatatcaaa
     2221 cctaattttc gtgattcatt attactggtc gcgcttgcca ccacgccatt gtggatgact
     2281 tcgctgtttt acctggtccc cagcctgagt ttcaatgtgc tggtgcatgg tgtggctgta
     2341 ttggcggcag tgctgttagt ctatcgcggg gtgcgttata tctttggtat ccgtgaacgc
     2401 ggggcattga tcaccttgag catggccatt gtatgcactg cggggcttgg ctttgccgtg
     2461 attctgatcg gctcactgat ctcgtgggag gctattgagc aattgcaatt cgcggtcaaa
     2521 aaaatgtaat gacaccctct caggatgaga gggacattgg cttgctggcg cctccgatag
     2581 gaggtgtcaa ctggtatgcc agcgcataat gaatgagcgc ggcattattc gaggcatcca
     2641 ttttcttgag aatttttgaa cgaaacacac tgaccgagcc gggcgcaatc gccagttcta
     2701 tggcaatggc attgactggc tggcccttga ttaacttaaa gaatacttgc agttcacgca
     2761 gtgacagccg ctcatgcagc aagtgacgct tgccgttcat catggcttgt tgttccggta
     2821 aacggtaagg ctgtttacgt gcaatcgttt tcatggctgc cagcacctgt gcgctatcgg
     2881 ttgagtaggg taaatagcca gcgcagccca ggcgtaaaaa atgcaccatg cgatccgtcg
     2941 attcgctgcc attgataatc agaatgggca agtcgggtgc catctgcttg agatcacaaa
     3001 atgtttccaa gccatttttc tcgggtgcag agacatccag gatcaccatg tcgtattgct
     3061 gtgtcttgat gagttgcagc gcgtcctgcg cattcgcaac ctcatcagca aggtgcagat
     3121 gtggatcttc attcagcagt tcacgcagtg cactatgact cagcgcgtga ccgtaaatca
     3181 ataatatatt tttcatggca tggtctactc atctttaatc tttcgttaag gtgcttacta
     3241 gcaccgtcat gttcgctgtt gtagtaattc cagcatactt tgttcagcca tgcttaatgc
     3301 aggccaatcg ttattagtcg cttgcataag cggcatccat ttacagccac acacggcgag
     3361 gtagctgtcg ataatgctgg gatgcacgta actatggcga cagatggctg gcgtattgcc
     3421 aagcctggaa gctgccagcc ggatagctgc gacgatgttt ttctttgctt ctgcctcact
     3481 gtggcagggc ggaaatgcgc tcagtgccac taaagtatgc aagctgccaa accaggttct
     3541 gaaatccttg gcggtgtaat catcgccgct caactggcgc aggtaatcgt tgacatctgt
     3601 cgaaccgatt gcatgctgta agccttcact atctacgtac tggaacaagg cttgccctgg
     3661 tagttcacgt aattggcgca tcatgcgcgc cagacacggg tcatgcagtt gcacgcgatg
     3721 ctcaacgcca cttttacccc ggaagtggaa ttccacgtca ttgttttcaa tggcaacatg
     3781 gcggttacgc aaggtggtaa ggccaaacga tttattcgtc ctggcataag cgtcattgcc
     3841 aatgcggatc agggtgtttt ccaataacct tatcatcatg gccagcactt tctgcttacc
     3901 catgccggat gtttttaaat cgcggtccac ttgttggcgg ataatgggaa gttgctggcc
     3961 aaaagcaacc atatgcgcgt atttcttgtt ctcgcgctgt aaacgccatg ctgcatggta
     4021 aagatactgc ttgcggcctt tggcatcacg gccggtcgct tgtaaatgtc cttgtggatg
     4081 cgggcatatc cacactgact cccacgccgg gggaattctc agcttggcga ttcgggacaa
     4141 cgactgtcta catttcaggc attgaccctg tgcgtccaga tacctgaatt tgcctcgcac
     4201 ccgctcacgg ctgaatccag gcgtgacttc ttgccggata taatgcagct tctccgggcg
     4261 agccgtaccg acagggctaa ttttctgaga tttcgatgcc tgtttgctca gcttcgctga
     4321 cgatttcctg tgtccgttct tgatgggccg ttttacggct atttccatta cctttgacat
     4381 tccagatcac attgcctaca tcatccgcca ctaataaatg ccctttttta tcgactaaca
     4441 cgccaaccgg acgtccttgt gcttgaccat cggtattcag gaattcggtc agcacatcaa
     4501 tcggctggcc agccggttta cctgcctcaa atggcacaaa aatgactttg tagccactgt
     4561 aagggcggcg attccaggag ccgtgctgtc cgacaaacat gccgttctta aactggtctg
     4621 gcaactgatt gcctttggcc ggggtaagcc ctaatgaagc agtatgtgag cccagcgcat
     4681 aatccggttt caccgccttg gcaaccagtt ccggcttttg cggctggata cggtcatcta
     4741 catgctggcc gtaatagctg tagggccagc cgtagaaggc accttgcgtc actgaggtca
     4801 tatagtctgg taccagatcg ctgcccagtt catcacgttc attgacgacc acccacaacg
     4861 cgccactatc tggctcccag gccatgccat tggggttgcg cagcccagaa gcataaatac
     4921 tgtgctgtcc agagtccggg tctacttccc agatagccgc ccgctctttt tctatctcca
     4981 tgccgttttc accgacattg ctattggagc cgatcgtcac gtaaagcttg ctgccatcct
     5041 gcttgggcaa gatgtttttg gtccagtggt ggttgcgctc agctggcagg cttgtgacct
     5101 tgatgggcgc agcggtaatt tctgtttgtc cctcctgata gggaaatttg accagcgcat
     5161 ctgcgttggc tacatagaat gtatcgccga tgagtgccat gccaaaaggg gagtgcaggt
     5221 cttgaataaa gatgtgccgc tcgtcggcga ccccgtcccc atcgttatca cgtagcagca
     5281 ggatacggtt catgctctct gccttggcac cgccatcttt cataaatttg ctggcaatcc
     5341 agcttttgag ctcgaatcca tcactttttt catgatggtt ggtttcggcc accagaatgt
     5401 cgccattcgg taactcgtat agccagcgtg gatgatgcaa gtcactggca aatgccacca
     5461 cgttagtccc aggcggcgaa gtcggctttt tgccttctgg ccagcccacg gcttcagcca
     5521 catggatggt cggcatcaag cctgtttggt aggaagaaag gtccgggtct gggccaaatc
     5581 ctgctgtttc aggcagtttg gctggttgag agcaagcact cagagcgaga atgccagcgg
     5641 aagcaatcag tcgcatgctg gattgtgaag gtggtttcga tggcatggtt cgctcctttt
     5701 tcagtgtctt tttatctgcg tgtggcaacc acctgatgga tgcccgtgac gccttgttca
     5761 aattgaatgg cgctggcggt catatacaat cgccagatcc ggtaagtgcg ttcaccgacg
     5821 aggctggcaa ctgtcgcgtg attggcttcc agcccggcca gccattgatg caaggtcatg
     5881 acataatggc gacgcaagct ttccacgtta aaaacgtcaa acttggccgc atccatctgc
     5941 tgcaaaaagt gagggaatgt ggacagatca ccttgtggaa acaactggcg gttgataaat
     6001 gcgctggacg catcttgttg cccagccaat tgctcgttgg taatgccgtg ggtcaggaac
     6061 aggccgccgg gtttcagggc ggcatgtacc ttggccaggt aatcgggaaa atgcgcctcg
     6121 ccgatatgat cggcaatgcc aatgcacgcc gctttgtcat agctgcctgt ggctggcagg
     6181 tcttgatagc tgcccaattc tattttcacc aggtgctcca gccccaatct tttcacctgt
     6241 ttggtggcat aggcatactg gctggcgttt tgcgtaatgc catggacaaa cacgccatag
     6301 tgcttggctg cccagcaggc gagtccaccc cagccacacc cgatatccag cagtgaatcc
     6361 ccatgttgca aatgcagctt gaggcagatc aggtccagct ggttacgttg tgcttgcgcc
     6421 aggtcacgcg tgtttcctga gaaataagca cacgaatgca gcatgtgttc gtcgagccag
     6481 cactggtaaa agtcttctgg cacattgtcg taactgagtg ctaccgggga tgaagtgtct
     6541 aattggtcgg gggtggggtc tatctgagga agagaggttt gttcaggttg ggcaaaatcg
     6601 gttgccagtg tcagcgcccg gaagatcaag cctaattttt cgtggagtgg caaagagagc
     6661 gattcaaaat gttcacgtaa ctgcatggcg gcgtgaaagt cgcccagtat ctggatctga
     6721 cccgagaaat aagcctctgc caggcattta gggtcgcaga accacaccat gttgcgtaag
     6781 acagacgctt gttgcaggca aaaggtaaat gctgggtcac cttctccaat attcagcagg
     6841 ttgccattcc ataatctcac cgaaaatgca cctggataat cctggaacag gcggttcagg
     6901 ataaagccag cctgtgaagt ttcaccgtta gcagcggttt gtacattttc atcccattgc
     6961 cacggctggg gttgatccag taatggtgtc tcaaactgca aggcggtcgc gaggtgatga
     7021 c
//
LOCUS       DATMSK010000165         4981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_129600,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000165 DATMSK010000000
VERSION     DATMSK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129600"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2996
                     /locus_tag="VNZ84_05545"
     CDS             <1..2996
                     /locus_tag="VNZ84_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU66454.1"
                     /translation="VIQTESPGLSASLVEKLVTQPIENVISGAIGLKSLRSQSIPGLS
                     IITVVFHDRSDIYRNRQIVAERLNMVGNRLPPGIVPNITPLTSSASTVMGLGVTSNKL
                     SLMELRTLVDWHLTPHLMTIPGIADINVYGGEVRQFQIKLDPRKLMLYQLNVQDVETA
                     ASRATGVRGAGYIQNDNQRIILNTQGQTTTPQQLANITLVRKLGRTIRIRDVAEVSEG
                     AAPSISAAAINGQPAVYLSVQGQLGANVYALTKQLELKLAEIKPSLDKQKVTLHEGLF
                     RPANFIEIAIQSLRSDILIGSVLVILVLFIFLFNVRTAIISATAIPLSLLTAIVVLYQ
                     SGVGLNIMVLGGLAIALGEVVDDAIIDTENIFRRLRENRLLSAPLPVTQVVFQASMEV
                     RSSVVYATIIVALVFLPLLTLSGVAGKLFAPLGYAYISAILASLVVALTLTPALCYVL
                     LARAPLKDEDSPLLKWLKKRYVRLLHQIEHRYQAILMSSFVCIALGLAILPLFRGQFI
                     PALREGHYILHMTAVPGTSEIQTLKLGNQVTKVLLDIPGVKSVAQWVGRAPNAADTFG
                     THYSEFEVEIGTVSGKEQERIFRAIREELAGETEDEDNDGKIEPGFVGVNFAINTFLT
                     ERIEETISGYAAGLVINIFGQDLDALDRDAQKVAGLLSGIRGIRDVIVQSPPETPEIV
                     IRLKPEKLALWGLQPVDVLDTVRACYEGVPVNHVFMGNRSIGVSVLLNDTSRDDIADM
                     RNIPIFNAEGQMLKLSDVAYVAQEGGRSKILHAGAKRIQTITANIAGRDQALLIADIR
                     ATLHNKLQLSSGNYLEFSGEAEANAQSRQDLVIHSLVAGVAIFLMLYIAFGRLRNLML
                     TFANLPFALIGGVVATFFTGGWISVGTLVGFVTLFGITLRNSIMMVSHFQHLVNKEGC
                     TWNLETCIRGASERLPSVLMTALVTALGLLPLAIGSGEPGREIEGPMATIIVGGLISS
                     TILNLLILPTIMLHFGRFEKSDHLE"
     gene            complement(3048..3704)
                     /locus_tag="VNZ84_05550"
     CDS             complement(3048..3704)
                     /locus_tag="VNZ84_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66455.1"
                     /translation="MWVIKLGGSVTHYGSLVQWLQLVVRWGDGKVIIVPGGGLYADAV
                     RDFQRMRAALPQGHINDSQAHALAIYAMDQMARSLVAMVPELALVRNPLEIAESGWQH
                     RGLVWLPSEMALNPVLANELALPETWEVTSDSLAAWLAWQLEANHLLLVKSDDRLLEM
                     RDGLQLGKLQALDIVDQGLSRLLTQAKFQTWIAHQTQYKLFEQGFSVNLPQDYLLHSE
                     "
     gene            complement(3722..>4981)
                     /gene="pabB"
                     /locus_tag="VNZ84_05555"
     CDS             complement(3722..>4981)
                     /gene="pabB"
                     /locus_tag="VNZ84_05555"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HWU66456.1"
                     /translation="VAAPVAKITHRRPYTQVSVNDSVQQSQQDPFFEIQQWLSSHHME
                     RVADIPFAGGALGYFAYDLGRSIERLPELALEDMPVPELLVGIYDWAIVVDHHLASTK
                     LVSHLRFGTHEALETLHQALLQPRNEEGAEPFTVISDIEPNMNQETYAQAFQQVKEYI
                     RAGDCYQINLAQRFAVRVKGDVCQAYDQFRQLSRAPFMAFLQVEDASGTPFAVLSMSP
                     ERFLQVIDQKVETRPIKGTRPRHMDAVMDQQAAEELAHSIKDRAENLMIVDLLRNDIG
                     KVCEVGSVKVDALFKLQKFTNVHHLVSIVRGILAPGFNALDLLRGCFPGGSITGAPKL
                     RAMEIIEELEPHRRGIYCGSIGYIGFDGCMDSNIAIRTAVVHNQQMTFFAGGGVVADS
                     DCDKEYQETFDKASQFFKLVAQSKANN"
BASE COUNT         1200 a         1355 c         1194 g         1232 t
ORIGIN      
        1 tcgtgataca aaccgaatcg cccgggctct cggctagcct ggtcgaaaaa ctggtcaccc
       61 agcccataga aaatgtcatt tccggcgcca tcggtctcaa aagcctgcgc tcgcaatcca
      121 tccctggcct gtcgattatc accgtcgttt ttcatgatag atcagatatt taccgtaacc
      181 ggcaaattgt cgccgaacgg ctgaacatgg tcgggaaccg cctaccgccg ggcattgtgc
      241 ccaatatcac accactcacc tcctctgcca gcacagtcat ggggttgggc gtcacctcaa
      301 acaagctgtc gctgatggag ttacgcacat tagtagactg gcatttaacc ccacatttaa
      361 tgacgatccc gggcatcgca gacattaatg tctacggtgg cgaagtgcgc cagttccaga
      421 tcaagttgga tccgcgcaaa ctgatgcttt atcagctgaa tgtgcaggat gtagaaacag
      481 ccgccagccg tgccaccggt gtgcgtggtg caggctatat ccagaacgac aaccagcgca
      541 ttattctgaa tacacaaggc caaacgacca cgccacaaca actcgcaaac atcacgctgg
      601 tgcgtaagct gggccggacc atacgcatta gggacgtcgc cgaggtcagt gaaggtgcgg
      661 ccccttctat cagtgccgcg gccatcaacg gccagccagc ggtttacctg tcggtacaag
      721 gacaattagg tgcgaatgtc tacgccctga ccaagcaact tgaactcaag ctcgcagaaa
      781 ttaaacctag cctggacaag caaaaagtca ctttgcatga agggctgttc cgcccggcca
      841 actttattga aatcgcgatc cagagtttgc gcagtgacat tctgatcggc tctgtactgg
      901 tgatcctggt gctgttcatc tttttgttta atgtgcgaac agccattatc tctgcgaccg
      961 ccattccgct atctttgctt acagccatcg tcgtactgta tcaatctggc gtgggcctca
     1021 acatcatggt actaggaggg ctggccatcg cactgggcga agtcgtggac gatgccatca
     1081 ttgatactga aaacattttc cgccgtttac gtgaaaatcg cttgctatca gcgccgttac
     1141 ccgtgaccca agttgtattt caagcctcta tggaggtgcg cagctcggtc gtctatgcca
     1201 cgattattgt cgctctggtg tttttaccat tactgaccct ctccggagtt gccggtaaac
     1261 tgtttgcacc actggggtac gcttacatct cggccattct ggcctcactg gttgtcgcac
     1321 tcaccctgac ccctgccctt tgctatgtct tactggcgag ggcacccctt aaagatgaag
     1381 actccccctt actgaaatgg ctgaaaaaac gctatgtcag attactgcat caaattgaac
     1441 accgctatca agccattttg atgagcagtt ttgtctgcat cgccttgggc ttggcgattc
     1501 tgcctttgtt caggggccag tttattcctg ctttgcgtga aggccattat attttgcaca
     1561 tgacggccgt cccgggcacc tctgaaatac aaaccctcaa actcggcaac caggtgacca
     1621 aagtcttgct ggacattcct ggtgttaaaa gcgttgcgca atgggtaggc cgcgcgccca
     1681 atgccgcaga tactttcggc acgcattaca gtgaatttga agttgaaatc ggtaccgtat
     1741 ccggcaaaga acaggaacgt atcttccggg caatccgcga agagctcgct ggggaaacgg
     1801 aagatgaaga taatgatggc aaaatcgaac ctggctttgt cggggttaat ttcgccatta
     1861 ataccttttt aaccgagcgt attgaagaaa ccatttccgg ttatgccgcc ggtttggtca
     1921 tcaatatttt cggccaagac ctggatgcgc tagacaggga tgcgcaaaaa gtggctggcc
     1981 tgctcagtgg tatccgtggt atacgcgacg tcatcgtgca gtcacctccg gaaacgcctg
     2041 agattgtgat ccgcctcaaa ccggaaaagc tggcactgtg gggcttgcag ccagtagatg
     2101 tgctcgatac tgtcagggcc tgctatgaag gtgtaccggt caaccatgtg tttatgggca
     2161 accgctctat aggcgtgagt gtattgctca atgatacctc acgcgacgac attgccgata
     2221 tgcgaaatat cccgattttt aacgcagaag gccaaatgct gaagttgtct gatgtggcct
     2281 atgtcgcaca agaaggtggc cgctccaaaa ttctgcatgc cggcgccaag cgcatacaga
     2341 ccatcacggc caatattgcc ggacgcgacc aagcgttgct catagcggat atccgcgcca
     2401 ctctgcataa taaactacag ttatccagtg ggaactacct ggaattttcc ggtgaggcag
     2461 aagccaatgc acaatcccgg caggatctgg ttattcactc gctggtagct ggtgtcgcga
     2521 ttttcctcat gctttacatt gcctttggcc gcttgcgcaa cctgatgctg acatttgcta
     2581 acctgccctt tgcgctcatt ggtggcgtag tcgcgacttt ctttaccggg ggctggatct
     2641 ctgtgggcac attggttggc tttgtgacgc tattcggcat cacactgcgc aattcgatca
     2701 tgatggtttc gcactttcag cacctggtaa acaaggaagg ttgcacctgg aacctggaaa
     2761 cttgtattcg tggggcatct gaacgcttac cctcagtgtt gatgacagca ttagtcaccg
     2821 cactgggcct gttgccgctc gccatcggca gtggcgagcc aggtcgcgaa attgaaggac
     2881 caatggcaac gattattgta ggcggcctga tctcgtctac tatccttaac ttgttgatcc
     2941 tgccaacgat catgctgcat tttggccgat ttgagaaaag cgatcacctt gagtgagttc
     3001 ctgaacaatc ctgattaagc gataaaaatc aaccgctcta gtgacagtta ctcgctatgc
     3061 agcaaatagt cctggggcag attgactgaa aagccttgtt caaacagctt atattgggtt
     3121 tgatgcgcta tccaggtctg gaatttggcc tgtgtgagca atcttgataa gccttgatcc
     3181 acaatatcca gcgcctgaag ttttccgagt tgcaaaccgt cacgcatctc taataaccga
     3241 tcatcagatt taaccaagag taaatggttg gcttcaagtt gccaggccag ccaggcggcc
     3301 aggctgtctg atgtcacctc ccaggtttca ggcagagcca attcattggc gagcaccgga
     3361 ttcaacgcca tttcactcgg caaccatacc aacccacgat gttgccagcc actttctgca
     3421 atttcaagcg gattacgtac cagcgccagc tcaggcacca tcgccaccag actacgtgcc
     3481 atttgatcca ttgcataaat cgccagtgca tgtgcttgac tgtcattaat atgcccttgt
     3541 ggcaacgcgg ctcgcatgcg ctggaaatca cgcacggcat ctgcatacag gccaccccca
     3601 ggcacgataa tgactttacc atccccccag cggacaacca gttgcagcca ttgcaccagg
     3661 ctgccatagt gtgtcacgct accccctagc ttgatcaccc acatattgtt attcccgagt
     3721 tttaattatt tgctttagat tgtgccacca atttgaaaaa ctgggaggct ttatcaaatg
     3781 tttcctggta ctctttgtcg cagtctgagt ctgcaaccac accccctcca gcaaagaatg
     3841 tcatctgctg attgtgtaca acggctgtgc ggatggcaat attgctatcc atgcagccat
     3901 caaagccaat gtagccaata ctgccacaat aaatcccgcg ccgatgaggc tccagctctt
     3961 caatgatctc catggcacgt aatttaggcg ctccagtaat ggagcctccg gggaagcatc
     4021 cccgtagtag atccagtgca ttaaagccag gcgccagtat cccacggaca atacttacca
     4081 gatgatgcac attcgtgaac ttttgcaact tgaacaaggc atcgactttc actgagccaa
     4141 cctcacatac cttgccaata tcgttgcgca gcaaatcaac gatcattaaa ttctctgctc
     4201 tatccttgat actatgcgcc aactcctcag cggcctgttg atccattacc gcatccatat
     4261 gcctggggcg tgtgccttta atcggacgtg tttccacttt ctgatcaatc acttgcagaa
     4321 atcgttcggg tgacattgac agtactgcaa atggggtgcc actggcgtcc tccacttgta
     4381 aaaaagccat gaagggcgcc cggctcaatt gccggaattg atcataagcc tggcagacat
     4441 ctcctttgac acgcacggca aagcgctgcg ccagattaat ctggtaacaa tcccctgccc
     4501 ggatatactc cttaacctgc tggaaagcct gcgcataggt ctcctggttc atattcggct
     4561 caatatcgct tatcactgta aaaggctcag cgccttcttc attacgaggc tgcaataagg
     4621 cttggtgtaa tgtttccaat gcctcgtgag taccaaaccg cagatgtgaa accagcttgg
     4681 tactggcgag atgatgatcg acgactatcg cccagtcata aatgccaacc agcagttcag
     4741 gcacaggcat atcttcgagc gccagctcgg gcaatcgctc tatactgcgc ccgagatcat
     4801 aagcaaagta gcctagcgcg ccgccagcaa atggaatatc ggcaacacgt tccatgtggt
     4861 gcgatgacaa ccattgctgg atctcaaaaa aaggatcttg ctgggactgc tgtaccgagt
     4921 cattcaccga gacttgagta tatggtcgac gatgggtaat tttggcgaca ggtgccgcga
     4981 c
//
LOCUS       DATMSK010000166         8567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_36098,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000166 DATMSK010000000
VERSION     DATMSK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36098"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1323
                     /locus_tag="VNZ84_05560"
     CDS             115..1323
                     /locus_tag="VNZ84_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU66457.1"
                     /translation="MAGAFGAAVMMNMVPAAIRSQAWAAGSDAPEITEVKVGFIPLTD
                     CAPIVVAAQMGFDKKYGIKIVPSKEASWAGVRDKLANGELHAAHVLYGLIYGVQLGIG
                     GQQKDMNILMALNNNGQGITLSNQLKEKGVTSGATLKRLIDNENRDFTFAQTFPTGTH
                     AMWLYYWLATYGINPMQDVKTIVVPPPQMVANMRIGNMDGYCVGEPWNARAIYDKVGY
                     SVATSQDIWTDHPEKVLGTTAEFVAKNPNTALALTKAILEACRYIDATKNRPEVAKLI
                     AGKAYVNAPEEVIEGRFLGHYDNGIGKKWEDPNYMKFFNDGKVNFPYLSDGMWFLTQH
                     KRWGLLKSDPDYLGVAKKVNRIDIYTEAAKSLGISVPADVMRTSKLIDGVVWDGKDPK
                     AYANSFKVKA"
     gene            1378..2271
                     /gene="ntrB"
                     /locus_tag="VNZ84_05565"
     CDS             1378..2271
                     /gene="ntrB"
                     /locus_tag="VNZ84_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HWU66458.1"
                     /translation="MQESQPAAPAATTKPIAAVVTPLSTTAAVATPSLQRRGTWAATA
                     KMAFEWLLPPVIGLIIFGAIWATVSALTQGLPGPVKTWHSAVELFSDPFYINNPNDMG
                     IGWNILNSLKRVATGFALAAIVGIPMGFVIGRYAFMSRMAAPIISLLKPVSPLAWLPI
                     GLLVFKAANPAAIWVIFITAVWPMIINTAVGVSKVPQDYMNVAKVLNLSEWKVVTKIL
                     LPYVMPYMMTGVRLSIGAAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVEHIIIAIFIV
                     GIVGLLLEQLLVLVAKRYSYE"
     gene            2295..3104
                     /locus_tag="VNZ84_05570"
     CDS             2295..3104
                     /locus_tag="VNZ84_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882909.1"
                     /note="This model describes the ATP binding subunits of
                     ATP-binding cassette (ABC) transporters for nitrate
                     transport, or for bicarbonate transport, in bacteria and
                     archaea; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter ATP-binding protein"
                     /protein_id="HWU66459.1"
                     /translation="MTSNVMQDRYVLIENVNMTFQTKKGSFQALKDINLNIREGEFIS
                     IIGHSGCGKSTVLNLIAGLLQPTDGYLFCAGREIAKPGPERAVVFQNHSLLPWLTCFE
                     NVYLAVERVFGDKEGQAQLADRTRAALELVGLSHAMEKRPNEISGGMKQRVGIARALA
                     MEPKVLLLDEPFGALDALTRAGLQDELMSIMARSGCTAVMVTHDVDEAVLLSDRIVMM
                     TNGPAATIGEILEVDLPRPRKRLELVEDAGYTHLRSEVLKFLYERHAKKAA"
     gene            3187..4623
                     /locus_tag="VNZ84_05575"
     CDS             3187..4623
                     /locus_tag="VNZ84_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HWU66460.1"
                     /translation="MNANAHPSLRQVSVAILLAALSMQAMSEEEALPEYTFMDAIKTG
                     KNMTSFRLRYENVQQDGLQPGTYTNASNTPNPTGTQDLKDANALTLRSLIGWQTAPYH
                     NWSFAAQLINVSKLDDDFNDGTNNFRTNGASNQNSRIEYAKVADPDHTDINQLYVDWT
                     GIKNTRVRAGRQQVNLDNVRFIGDIAFRQVMQVFDGVSVFNKSIPDTELFLAHFGKVS
                     QIFTTERTGDLEVINARYRLSPTESLIGYGYLSNFDNLGMGNAWFGTGAANLAANQSN
                     KIIGIRLDGTHPFTPNYRAHYTAEYAKQSDYKGGDDRIDAHYYKLGGGFGIDNFNLRI
                     DQELLSSNNNQYAFQTPFGTNHLFQGWVDKFLVTPRSGIKDSFVTATYRYGDFVFFAD
                     YHVLDSDENFYTVNGGTARNGNRYGTEWNAAVTWNVDKNWMTKLEYGKFSEDDHFAAA
                     ANTTSNVAGTRGRIRDTEKLWLTAMYTF"
     gene            4642..5427
                     /gene="cobA"
                     /locus_tag="VNZ84_05580"
     CDS             4642..5427
                     /gene="cobA"
                     /locus_tag="VNZ84_05580"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWU66461.1"
                     /translation="MTSAAKVYLVGAGPGDPDLITLKAIKALQQAEVVLVDDLVNPAL
                     LTHCPQARIVPVGKRGGCKSTPQHFINRMMVALAEQGETVVRLKGGDPFVFGRGGEEM
                     LALRAANIPYEIVPGMTSGLSACASMEVPVTHRGVNHGVTLITGHTQDGGPLPWQALV
                     ESNTTLVIYMGMQHLPDISRALINAGMSAEMPCMVVENGTRSDQRAVTSSLQSMPYLA
                     HQEVMKSPAIIVVGEVVKLARNTQQESDWQHNVQTWLNMATAA"
     gene            5493..6704
                     /locus_tag="VNZ84_05585"
     CDS             5493..6704
                     /locus_tag="VNZ84_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HWU66462.1"
                     /translation="MKKSFWEVGHKPTLFSAFFYFDLSFLVWVMLGPLAVQIAADLQL
                     EPAQKGLMVATPVLTGAILRIVMGVLVDQLKPKKAGLIGQAIVILSLLTAWLHGIHTY
                     QQALLLGVFLGFAGAAFAVALPLASRWYPPEHQGTALGIAGAGNSGTALAALFAPGLA
                     VAFGWSNVFGLALIPLALALIVYVLMAKDAPDAPAPKSMSQYLSVLKDKDAWWFMFFY
                     SVTFGGFVGMASSLTIYFHDQYGLDPVHAGYFTAACVFAGSLVRPFGGLLADRIGGIR
                     SLSAMYILAALFLFILSAGIETQLIGLGVVMLAMAALGMGNGAVFQLVPQRFRKEIGV
                     MTGLVGMAGGVGGFYLASSLGYSKQITGSYQVGLTIFAGLAILALIGLSNVKNRWRTT
                     WGAATVTSAKI"
     gene            6743..8431
                     /locus_tag="VNZ84_05590"
     CDS             6743..8431
                     /locus_tag="VNZ84_05590"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HWU66463.1"
                     /translation="MGLIFSVGESSLTGPRARNEDYIGIVTPADAQLAIKGALLAVAD
                     GISGGAGGAEAAEMTIRTLSADYYATPDTWTIQHALEKVVQAANRWVLSQARKQSEMA
                     GMATTLSAMVLRGQRYVLAHIGDCRVYRLREQSLQCLTTDHVWDRPDMRHVLKRAVGL
                     DDQLSVDYAEGLLQPGDIYAIVSDGVWAALGDAEIHKALMLYHTPQLAAEHLTRQALE
                     KGGQDNASAVVVSIENTGQDHLTDMLADAHTLPIPPKLSVGSQIDGFTVTQLIQDTRV
                     SILYQVKNTAGQLFVLKTLQPDRADDKATCEALLNEEWLAKRVVSQCVPQILPLTRDQ
                     RTALYYVMTWHAGFTLQQRLNAEHHFTINGVANIGQELLRGLSMLHRLNIVHRDIKPA
                     NIHMGSDNRLRILDLGVALNTTLPMPEQMENPGTPSYMAPELFDGKVADRQADIYAAG
                     VTMYHLLTRKYPYGEIEPFQSPRFGEPIRPSRYRPEIPAWLENIILKAIARKPTDRFE
                     TAEEMLLALEHGELRPVVIPRTPLIARARLVKWQWLAVCSLLANIILIYLLFVS"
BASE COUNT         2045 a         2286 c         2284 g         1952 t
ORIGIN      
        1 aacatgagca gttctgatga aaaaacaacg gttgtaccag tgagcgttca gccaacgtta
       61 gcaaacgaac catccgctgc ggatcaaagc cgccgtgcgt ttttccgcca agccatggcc
      121 ggtgctttcg gtgctgcagt catgatgaat atggtgcctg ctgccattcg tagccaggct
      181 tgggcagccg gttcagatgc gccggaaatc actgaagtga aagtcggttt catcccgctg
      241 acagactgtg caccgattgt ggtggctgca caaatgggct tcgacaaaaa atatggcatc
      301 aagattgtgc cctccaagga agcttcctgg gctggcgtac gcgacaagct ggcgaatggt
      361 gaactgcatg ccgcccacgt gttgtacggc ctgatttacg gcgtacaact gggcatcggt
      421 ggccagcaaa aagacatgaa tatcctgatg gcgctgaata acaatggcca gggcatcacc
      481 ctgtccaacc agctaaaaga aaaaggcgtg accagtggcg cgaccctcaa acgactgatt
      541 gataacgaaa accgcgactt tacctttgca caaaccttcc ccactggcac ccatgccatg
      601 tggttgtatt actggctggc cacttacggc atcaacccga tgcaagatgt caaaaccatt
      661 gtggtccctc cgccacaaat ggtggccaac atgcgtatcg gcaatatgga tggctactgt
      721 gtaggcgaac catggaatgc gcgtgcgatt tacgacaagg ttggttactc agtagccacc
      781 tcacaagaca tctggaccga tcacccggaa aaagtattgg gcaccaccgc cgagtttgtg
      841 gccaaaaatc cgaatacagc actggcactg accaaagcca ttctggaggc ttgccgttat
      901 atcgatgcca ccaagaaccg tcctgaagtg gccaagctga ttgcaggcaa agcctatgtg
      961 aatgcacctg aagaagtgat tgaaggccgc ttccttggcc attacgacaa tggcatcggc
     1021 aaaaagtggg aagaccccaa ctacatgaag tttttcaatg acggcaaggt taacttccct
     1081 tacctgtctg atggtatgtg gttcctgacc cagcacaaac gttggggctt gctgaagtct
     1141 gaccctgact acctgggtgt agcgaaaaaa gtgaaccgca tcgatatcta caccgaagcg
     1201 gcgaaatctc tgggcattag cgtgccggcc gacgtcatgc gtacgagcaa gctgatcgac
     1261 ggtgtggtgt gggatggcaa ggatcctaaa gcctatgcca acagctttaa agtgaaagcc
     1321 taatcttttt taaggagaac acacatgagt gtcgtcagct tttacaagga aaaaaacatg
     1381 caagaaagtc agccggctgc tcccgcagcc acgacaaaac cgattgctgc cgtggttaca
     1441 cctctctcaa ccactgctgc agtagccacc ccgtctttgc agaggcgcgg gacatgggcg
     1501 gcaacagcca aaatggcgtt tgaatggttg ttgccgcctg tgattggttt gatcattttt
     1561 ggcgcgatct gggccacggt gtctgccctg acacaaggcc tgcccggccc ggtaaaaacc
     1621 tggcactccg ccgtcgagtt attcagtgat ccgttttata tcaacaatcc taatgacatg
     1681 ggcatcggct ggaacatcct gaactcgctc aagcgcgtgg ctaccggatt tgcactggca
     1741 gcgattgtcg gcattcccat ggggtttgtc attggacgtt atgcatttat gtccaggatg
     1801 gcagccccca tcatcagcct gctgaaaccc gtctcaccgc tggcctggtt gccgattggc
     1861 ctgctggtat tcaaggccgc taacccggca gcgatctggg tgattttcat tacagcggtc
     1921 tggcccatga tcatcaatac cgctgtcggt gtcagcaaag tgccacaaga ctatatgaat
     1981 gtggccaagg tgctcaattt gtccgaatgg aaagtggtca ccaaaatcct gctgccgtat
     2041 gtcatgccct acatgatgac tggcgtacgc ctctccatcg gggcagcctg gctggtgatt
     2101 gtcgcagcag agatgctgac cggtggcgtg ggcattggtt tctgggtgtg ggatgaatgg
     2161 aacaacctga atgttgaaca catcatcatc gccatcttca ttgtcggcat tgtcggcctg
     2221 ttactggagc aattactggt actggtcgct aaacgctaca gttacgaata accctcggga
     2281 aaaggataaa gattatgacg agcaatgtca tgcaagaccg ttatgtcctg attgaaaacg
     2341 tcaacatgac ctttcaaacc aaaaaaggca gttttcaggc cctgaaagac atcaacctga
     2401 atatccgtga aggcgagttt atctccatca tcggccactc tggctgcggc aagtcgaccg
     2461 tgcttaacct gattgccggc ttgttgcaac cgacagatgg ctacctgttt tgcgccggcc
     2521 gtgaaattgc caaaccaggc cctgaacgcg ctgtggtgtt ccagaatcac tccctgctgc
     2581 catggctcac ctgttttgaa aatgtgtatc tggcggttga acgtgtattt ggcgacaaag
     2641 aaggccaggc acaactggca gatcgcacgc gtgccgcact ggaactggta ggtttaagcc
     2701 atgccatgga aaaacggcct aacgaaatct cgggcggtat gaagcagcgg gtcggtattg
     2761 cccgcgcact ggccatggag cctaaagtcc tgttactgga tgagccattt ggtgcattgg
     2821 atgccctgac ccgtgcaggt ttgcaggacg aactcatgag catcatggcc cgttcaggct
     2881 gcactgcggt aatggtcacc cacgatgtcg atgaagccgt actgctgtct gaccggattg
     2941 tcatgatgac caacggccca gccgcgacga ttggtgaaat cctggaagtg gatttaccac
     3001 gcccccgcaa gcgcctggaa ctggtagaag acgcgggcta cacgcatttg cgcagcgaag
     3061 tcctgaagtt tttatatgaa cggcacgcca aaaaggctgc ataagatatg atgctgacgg
     3121 gcggccaccc gccagcatcc acacctcatc gaagtatggt taccccacat tgaaaaggag
     3181 caacacatga acgctaacgc acacccaagc ctgcgtcaag tttccgtcgc cattttgctg
     3241 gcggctttaa gtatgcaggc gatgtctgaa gaagaagcct tacctgaata cacttttatg
     3301 gatgcgatca aaaccggcaa aaatatgacc agtttcaggt tacgttatga aaatgtgcag
     3361 caggatggtc tgcaacctgg cacttacacc aatgcaagca acacgcccaa cccgacgggg
     3421 acacaggacc tcaaggacgc aaacgcatta accttgcgca gcctgatcgg ttggcaaaca
     3481 gcgccgtatc acaactggag ttttgccgcg cagttgatca atgtcagcaa gctggatgat
     3541 gactttaatg atggcaccaa caatttccgc accaacgggg catccaacca gaacagccgc
     3601 attgaatacg caaaagtggc tgacccagac cataccgaca ttaaccagct gtacgtagac
     3661 tggaccggca ttaaaaacac ccgtgtccgt gctggtcgcc agcaagtcaa tctggataat
     3721 gtgcgtttta tcggtgacat tgcttttcgc caagtcatgc aggtttttga cggtgtttct
     3781 gtgtttaata aatccattcc ggacaccgag ttattcctgg cacactttgg aaaggtcagc
     3841 cagattttca ccaccgaacg taccggcgac ctggaagtga tcaacgctcg ctatcgccta
     3901 tcccccactg agtccctgat cggctatggc tacctgagca attttgataa cctggggatg
     3961 gggaatgcat ggtttggtac tggtgcagcg aacctggcag ccaatcaatc taacaagatc
     4021 atcggcatcc gcctggatgg tacgcacccg ttcactccca attaccgtgc gcattacacc
     4081 gctgaatatg ccaaacaatc cgattacaaa ggtggggatg accgtatcga tgcccactac
     4141 tacaagctgg ggggcggttt tggcattgac aactttaacc tgcgtatcga ccaggagctc
     4201 ttatccagca acaacaacca gtatgcattc cagacaccat ttggcaccaa ccacctgttt
     4261 cagggctggg tagataaatt cctggtcacg ccacgatcag gcattaaaga cagtttcgtg
     4321 actgccactt accgttatgg tgattttgta ttttttgctg actaccacgt cctggactct
     4381 gacgaaaatt tctacaccgt caatggtggt accgcacgca atggcaaccg ctacgggaca
     4441 gagtggaacg ctgccgtcac ctggaatgtg gataaaaact ggatgaccaa gctggagtac
     4501 gggaaattta gcgaggatga ccactttgct gccgcggcca ataccaccag caatgtggcc
     4561 ggcacccgcg gacgtatccg ggataccgaa aaattatggc tgactgccat gtataccttc
     4621 taacccaata aggatttagc aatgacttca gccgcaaaag tatatttagt cggtgccgga
     4681 cccggcgacc ctgacctcat cacgctcaag gccatcaagg ccttacagca agccgaagtc
     4741 gtgctggtcg acgacctggt caatccggcc ctgctcacgc actgcccaca ggcacgcatt
     4801 gtgccggtcg gcaagcgtgg tggctgcaag tctaccccgc agcactttat caaccgcatg
     4861 atggtggcac tagccgagca gggagaaacc gttgttcgcc tcaaaggtgg cgaccctttt
     4921 gtgttcgggc gtggcggcga agaaatgctg gctttgcgtg cagccaatat cccctacgaa
     4981 attgttccag gcatgaccag cggcctgagt gcatgcgcca gcatggaggt gcctgtcacc
     5041 catcgcggcg ttaatcatgg cgtcaccctg attaccggcc atacccagga tggcggccca
     5101 ttaccttggc aagcgctggt ggaaagtaat accaccctgg tgatttatat gggcatgcag
     5161 catttgccag acatctcacg tgcattgatc aatgccggca tgtcagccga aatgccatgc
     5221 atggtggtgg aaaatggcac ccgcagcgat cagcgggcag tgacttcctc actgcaatcc
     5281 atgccttacc tggctcatca ggaagtcatg aaaagcccgg ccattattgt ggtgggcgaa
     5341 gtggtaaaac tggccaggaa cacacaacag gaaagcgatt ggcaacacaa tgtgcagacc
     5401 tggttaaaca tggcaactgc ggcataaacg catcaagctt gctatcggca tagacacaat
     5461 atttaatatg gtttaagaaa ccggagatga aaatgaaaaa gagtttttgg gaagttggcc
     5521 acaagccgac cctgttttca gcgttctttt attttgacct gagctttttg gtttgggtca
     5581 tgttgggacc attggccgta caaatcgcag cagacctgca attggaacca gcgcaaaaag
     5641 gcctgatggt cgcaacccct gtgctgaccg gggctattct acggatcgtt atgggcgtct
     5701 tggtcgacca gctcaagccc aaaaaagcgg gcttgatcgg ccaggcgatt gtcatcctgt
     5761 ctttgctgac tgcctggctg catggcattc atacctacca gcaagcctta ttgttaggtg
     5821 tgttcctggg ctttgccggc gctgcatttg ccgttgcatt gccgctggct tcccgctggt
     5881 atccacctga gcatcaggga accgcgcttg gtatcgctgg cgcaggaaat tctggtacgg
     5941 ccctggcagc gctgtttgcg cctggcctgg ccgtcgcttt tggctggagc aatgtatttg
     6001 ggctggctct tattccgctg gcactggctt taatcgtgta tgtattgatg gcgaaagatg
     6061 cgcctgatgc cccagcgccc aaatccatgt cgcaatactt gtccgtgctg aaagacaaag
     6121 atgcctggtg gtttatgttt ttctatagcg tcacctttgg tggttttgtc ggcatggcct
     6181 cctctttgac catttacttc catgaccagt atggcctgga tcctgtgcat gccggttact
     6241 ttaccgcggc ctgtgtattt gccggctccc tggtccgtcc atttggtggc ctgctggctg
     6301 accgcatcgg tggcatccgc agcctgagtg cgatgtatat cctggcggcg ctcttcttgt
     6361 ttatattgag tgcggggatt gagacgcaac tcatcggttt aggggtcgtg atgttggcca
     6421 tggcggcatt ggggatgggc aatggggcgg tgttccagtt ggtcccacaa cgtttccgca
     6481 aagagatcgg cgtcatgacc gggttggtgg gcatggctgg cggcgtaggt ggtttttacc
     6541 tggcctccag cctgggttac tccaagcaaa tcactggcag ttatcaggtg ggcctgacta
     6601 ttttcgcggg cctggctatc ctggcgctga ttggcttatc caatgtgaaa aaccgctggc
     6661 ggacgacctg gggtgcagcc accgtcacca gtgctaaaat ctagtcatac tccagaacaa
     6721 ccaacaaaaa gggcgtagcc ggatggggct gatattcagc gtaggtgaaa gtagtttgac
     6781 cgggccaagg gcacgcaatg aggattacat tggcattgtc acgcccgccg atgcgcaatt
     6841 ggccatcaag ggcgccctgc tggctgtcgc cgacggcatc agtggcggtg ccgggggtgc
     6901 tgaagcggct gaaatgacga tacgtacgct ttcggcagat tattacgcca caccggacac
     6961 ctggaccatc cagcatgccc tggaaaaagt cgtgcaagcc gccaatcgct gggtcttgtc
     7021 acaagcacgc aaacaaagcg aaatggccgg gatggcaacc acgctttcgg ccatggtctt
     7081 gcgtggacaa cgctatgtgc tggcccatat cggtgactgc cgcgtctatc gcctgcgcga
     7141 acaatccttg cagtgcctga cgacagacca cgtttgggat aggcccgaca tgcgccacgt
     7201 actcaaacgc gcagtgggcc tggatgacca gctcagtgtc gattacgctg aaggcctgtt
     7261 acagcctggg gatatttatg ccatcgttag cgatggtgtc tgggctgcgc ttggcgatgc
     7321 ggaaattcat aaagcgctca tgttgtacca cacaccgcaa ctggccgcag agcacttaac
     7381 gcggcaggcg ctggaaaaag gggggcaaga caacgcttct gccgtggttg tcagcattga
     7441 aaacaccggc caggatcatc tgaccgacat gctggcagat gcccataccc tgccgatccc
     7501 tcccaaattg tcggtgggca gtcagattga cggatttacc gtgacgcaat tgattcagga
     7561 tacgcgcgtc agcatcctgt accaggtcaa aaatactgcc gggcagctgt ttgtattaaa
     7621 gacgctgcaa cctgaccgtg ccgatgacaa ggcaacctgt gaggccttgc tcaatgaaga
     7681 gtggctggca aaacgggtcg tatcgcaatg tgtaccgcag atattgccgc tgacgcgtga
     7741 ccagcgcacg gcactgtact atgtcatgac atggcatgca ggcttcacac tacagcaacg
     7801 gttgaatgca gagcatcact ttaccatcaa tggcgtcgcc aatatcggcc aggaattgtt
     7861 acgcggcctg agcatgctgc accgtctgaa tattgtgcac agggatatca aacccgccaa
     7921 tattcatatg ggcagcgata accgcctgcg gattcttgac ctgggtgttg cactcaacac
     7981 caccttgccc atgcctgaac agatggagaa tcctggcacg cctagctata tggcaccaga
     8041 actgtttgac ggcaaagtgg cagaccgcca ggcagatatc tatgctgcag gggtcaccat
     8101 gtatcactta ctgacacgta aatatcccta tggtgaaatc gagcctttcc agtctccgcg
     8161 ctttggtgaa cctatccgtc ccagccgtta tcgtccggaa attccggcct ggctggaaaa
     8221 cattatcctc aaagccattg cccgcaaacc gacagaccgt tttgaaactg cggaagaaat
     8281 gttactggcg ctggagcatg gtgagttgcg gccagtggtc atcccgcgca ccccactcat
     8341 tgcccgcgca cggctggtga aatggcaatg gctagcggtg tgctccctcc tcgccaatat
     8401 catcctgatt tacctgctgt ttgtttcctg atgccccctg caagggacac aaaaccactg
     8461 catcgtgatt gaaaggtcga ttctggtgca atcgcatcat aaccgtgcat gcgatggatg
     8521 acccaccctt aacactctca tttcccagac aaaccagtgg gcaattt
//
LOCUS       DATMSK010000167         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_82414,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000167 DATMSK010000000
VERSION     DATMSK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82414"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VNZ84_05595"
     CDS             <1..462
                     /locus_tag="VNZ84_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU66464.1"
                     /translation="VFDTNVMGLVHTFQPFISAMKKAAANGETAQLAGIASVAGIRGL
                     PGSGAYSASKAAAMTYLESLRVEMQLYDITVTTIAPGYIRTPMTDINTYPMPFLMDAE
                     TFADKALNAIAKQRRFTVIPWQMGILARLMRFLPAVIWDYIMKKAPHKARL"
     gene            654..893
                     /locus_tag="VNZ84_05600"
     CDS             654..893
                     /locus_tag="VNZ84_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66465.1"
                     /translation="MDKLALFYQKVMDNTHPAQHPLQPQAHEVWQDQCRICLVKGKLV
                     PLPYIEASRYDDYEFGATLAAINANRLIRKLVKKQ"
     gene            complement(897..1676)
                     /locus_tag="VNZ84_05605"
     CDS             complement(897..1676)
                     /locus_tag="VNZ84_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HWU66466.1"
                     /translation="MSAMQAANFNLQDYLARIGFQGVLAADIATLNALMRCQLFSVPF
                     ENLDVQAGKVVSIIPEDIVEKIVYQRRGGYCYEVNGVFAMALQALGFDYQLVAARPMF
                     YPVKRPKTHMAIVVHINQQAWLCDLGFGSYGIRAPMSLEVLDSEVMQDHDCFMLTATN
                     TDGFLLKAKVDQAWQPQFAFDLTPWELIDFYPANYMNSTHPDAIFVQKLLVVFHTQAG
                     RHILFGNTLKTIADGVVEKRILSAAERTDTLKTLFGLSLLD"
     gene            complement(1734..>3341)
                     /gene="ettA"
                     /locus_tag="VNZ84_05610"
     CDS             complement(1734..>3341)
                     /gene="ettA"
                     /locus_tag="VNZ84_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HWU66467.1"
                     /translation="QIIKNISLSFFPGAKIGLLGLNGSGKSTVLRIMAGADKEYEGEV
                     QWQPNLSIGYLPQEPQLDPDKTVRQEVEAGLGAIMEAKQKLEEIYAAYAEPDADFDKL
                     AEEQAKYENILAASGSDVEQQLEIAADALRLPPWDAMIGPLSGGEKRRVALCKLLLSK
                     PDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAVTHDRYFLDNAAEWILELDRGEGI
                     PWKGNYSSWLEQKEDRLALEQKQEDAHRKAMKQELEWVRQGAKARQSKSKARLARYEE
                     MSSVEYQRRNETQEIFIPPGERLGNQVIEFSNVSKGFGDRLLIDNLSFTIPPGAIVGV
                     IGPNGAGKSTLFRMLQGKEQPDSGEIITGPTVKLAAVDQTRDGLDNDKTVFEAISGGL
                     DILSVGKFEMPSRAYIGRFNFKGGDQQKIVGQLSGGERGRLHLAKTLISGANVLLLDE
                     PSNDLDVETLRALEDALLEFSGCVLVTSHDRWFLDRIATHILAAEGDSQWTFFNGNYQ
                     EYEADKKKRLGEEGAKPKRIRYKPITR"
BASE COUNT          836 a          892 c          766 g          847 t
ORIGIN      
        1 gtctttgata ccaatgtcat gggtctggta catacctttc aaccttttat ttctgcgatg
       61 aaaaaagccg ccgcaaacgg tgaaactgcg caactggccg ggatcgccag tgttgcagga
      121 atccgtggat tacctggctc cggggcttat agcgccagta aggcagcagc gatgacctac
      181 ctggaaagcc tgcgtgtcga aatgcagctt tacgacatta ctgtcacgac gattgcacca
      241 ggctatatcc gcacaccgat gacagatatc aacacctatc ccatgccatt tttaatggat
      301 gctgagacat ttgctgataa agctctcaat gctatcgcca aacagaggcg ttttacagtc
      361 atcccctggc aaatgggcat tttggcgcgc ctgatgcgct ttttacctgc agtaatatgg
      421 gattacatca tgaaaaaagc cccgcataag gcgaggcttt gatccatatt ttctcttcta
      481 gcttaggtct taaagcgttt cagcactccc attatttcgc ttaaaacgcc tatgcatctt
      541 accttaccaa cattgcacag cctctttaca tgaccaacga cttagacgta ttaatgcgaa
      601 cagatgtatt agtcctaaca ggctatttga gccaaaatgg ctaagagaga taattggaca
      661 aactcgcatt gttttatcaa aaagtaatgg acaacacgca ccccgcccaa caccccctac
      721 aacctcaagc acacgaagtc tggcaagatc aatgtcgtat ctgtttagtc aagggtaaat
      781 tggtccccct gccttatatt gaagcatcgc gctatgatga ctatgaattt ggtgccaccc
      841 tggcagccat caatgccaat aggctgatac gcaaactggt taaaaagcaa taggcttcaa
      901 tccaacagcg acaagccaaa taatgtttta agcgtatcag tacgctctgc ggcggataag
      961 attcgttttt ccacaacgcc atcggcaatg gtttttaagg tattaccaaa taaaatatgc
     1021 ctccctgctt gcgtatgaaa caccaccagc aacttttgta caaaaattgc atccggatgc
     1081 gtggagttca tatagttggc cggatagaaa tctatcaatt cccatggcgt caaatcaaag
     1141 gcaaactgcg gttgccacgc ctgatcaacc ttggccttga gtaaaaatcc atctgtattg
     1201 gttgcagtca acatgaagca atcgtgatcc tgcatgactt cgctatccag cacttctaaa
     1261 gacattggtg cacgaatccc ataactacca aaacccagat cacacagcca ggcctgttgg
     1321 tttatgtgca caacgatggc catatgggtt tttggccgct ttaccgggta aaacatcggc
     1381 ctggcggcca ccagttgata atcaaagccc aacgcctgta aggccatggc aaacacacca
     1441 ttgacttcgt aacaataacc accgcgacgc tgatagacaa tcttctcgac aatatcctca
     1501 ggaatgatgg acactacttt tccagcttgt acatccaggt tttcaaacgg cacactgaac
     1561 agctggcaac gcatcaaggc gttcagcgta gcaatatctg cagcgagcac gccctgaaag
     1621 ccaatacgtg cgagataatc ttgcaggttg aaattagcag cttgcatagc actcatgtgt
     1681 cttgccatat taaaagttga aaaaagcccg ctttaagagg cgggctttgt atattaacgc
     1741 gtaatcggct tgtagcggat gcgcttgggc tttgcccctt cttcacccag ccgttttttc
     1801 ttgtcggctt catattcctg atagttaccg ttaaaaaacg tccactgtga atcaccctct
     1861 gccgccagga tatgcgttgc aatccggtcc aggaaccagc ggtcatgtga ggtgaccagt
     1921 acgcaacctg agaactccag cagtgcatct tccagcgcac gcagcgtttc cacgtcgagg
     1981 tcgtttgaag gttcatccag cagtaatacg tttgcaccgg aaatcagcgt ttttgccaaa
     2041 tgcaagcggc cacgctcacc acccgagagt tgaccaacga ttttctgttg gtcaccaccc
     2101 ttgaagttga agcggccaat ataggctcgg ctaggcattt caaatttgcc cacactgagt
     2161 atatcaagcc caccggaaat cgcttcaaac acggttttgt cgttatccaa gccatcgcgc
     2221 gtctggtcca cggcggccaa tttgacggta ggcccggtga tgatttcacc gctatctggc
     2281 tgctctttgc cttgcagcat gcggaacaag gtagatttac cggcaccgtt agggccgatc
     2341 acacccacaa tcgcgccggg cggaatcgta aatgacaggt tatcaattaa taagcggtca
     2401 ccaaagcctt tggacacatt gctaaattca atgacctggt tacccaagcg ctcaccgggc
     2461 ggaataaata tttcctgcgt ctcgttacgg cgctggtact caacgctgga catttcttca
     2521 taacgggcca gacgcgcctt gcttttgctc tggcgggctt tagcgccctg gcgtacccat
     2581 tccagctctt gcttcatggc tttacggtgg gcatcttcct gtttctgctc cagggccagg
     2641 cggtcttctt tttgttccag ccagcttgag taattgcctt tccatggaat cccttcgcca
     2701 cgatccagtt ccaggatcca ttcggctgca ttatcgagga aataacggtc gtgggtgaca
     2761 gccaccacag tgcctgggaa acgcacgagg aactgttcca gccattcgac agattcagca
     2821 tccaggtggt tggtcggttc gtccagcaac agcatatcgg gcttggacaa tagcaactta
     2881 cacaaggcga cacggcgttt ttcaccgcct gataatgggc ctatcatggc atcccatggc
     2941 ggcaggcgca gcgcatcagc ggcgatttcc agttgttgtt ctacatcgct gccgctggcg
     3001 gcgaggatat tctcgtactt ggcctgctct tcggccagct tgtcaaagtc ggcatcgggt
     3061 tcagcatacg ccgcgtaaat ctcttccagt ttttgcttgg cttccataat ggcacccaaa
     3121 ccagcttcca cttcctgacg cacggtttta tcggggtcca gctgcggctc ctgcggcaag
     3181 tagcctatgg acaaattggg ctgccactgt acttcgccct cgtattcttt gtcagcaccg
     3241 gccatgatgc ggagtacggt ggacttacct gagccattga ggccgagcag gccaattttg
     3301 gcacccggaa aaaatgacag ggaaatgttt tttatgattt g
//
LOCUS       DATMSK010000168         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_223617,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000168 DATMSK010000000
VERSION     DATMSK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223617"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VNZ84_05615"
     CDS             <1..145
                     /locus_tag="VNZ84_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HWU66468.1"
                     /translation="KVKTTGGQGSGILSSMSQANCFIVLDERSGPLEAGTLVEVQPFY
                     GIC"
     gene            334..1017
                     /locus_tag="VNZ84_05620"
     CDS             334..1017
                     /locus_tag="VNZ84_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWU66469.1"
                     /translation="MFTLPKPDYDLPPPRKQEIRIELVKEQPPAPVQPAAEPEPTPPK
                     PIPEPPKPQPTPVKQEQKPIKHDPIPQKPTPVPEPVVEAPRPAVDSPTPPVIAAKPTV
                     SEAKPEVVVPPAPPPEPVKAGPSEGDIEAARNAFRNAAHRELKKNQRYPRIAQDRGIE
                     GDVKLSINMDDHGNVTGIEIVESSGNKAIDDAAMTAAQKSQLKQHFTEILHGKINNII
                     VTVSFKLSS"
     gene            1024..2499
                     /locus_tag="VNZ84_05625"
     CDS             1024..2499
                     /locus_tag="VNZ84_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWU66470.1"
                     /translation="MFFKRTSSSINGAHDKPLRYLLLFHDLYFIALIILAVASGGYGI
                     YLWDKASTQSQRINFVIQEIYLVRGDLYRQTKELFDAFFLQEENALHEYNQYTTSILA
                     HLERLQNIAIDQQEREALMEIERKYRSLVNEAPALFYRYQNNPNRKAQKSIYQDIETG
                     IFRHYEEVSKRAEYLLMVKQGEIEHRLDDARSNSIAVLSMPLILACILIIYSRRILKN
                     SIVRPINDIMQATNAISAGNLSHQVPEAGAEELAVLSREINKMAEDLAASRDALVKSE
                     KQAALGLLVPMLAHNIRNPLASIRATAQVIDDPLLDKDTLESIQGIIHTVDRLERWTG
                     SLLSYLHPIKPMLNQLLLSSIVQGAISTLQPKLDEKQIKVLENSANIKRLILTDEHLL
                     EQMLYNLLLNASEASPKGAEIQLRCTLENNLLMLVIIDQGPGMPFKPDPHALTPGPTT
                     KRFGTGLGIPFAFKVCEELGGAMSFESEAGSGTRITITLPQ"
BASE COUNT          737 a          688 c          618 g          607 t
ORIGIN      
        1 gaaagtgaaa accacaggag gacagggctc aggcatctta agtagcatga gccaggctaa
       61 ttgctttatt gtgcttgatg aacgctcagg cccgttagag gctgggacgc tggtcgaagt
      121 ccaaccattt tatggcattt gctaaaccac taaccaagct gggaatactt gcacgcgctg
      181 gcaagaatta tcaaaaatag cttatagtta cgggtcgcgg tgggggttaa tcataaggat
      241 agtggcgtgc aagccgtttt aaaagagtca agacagttta atcatttatt agagtggtgc
      301 ctcgttgctt cgctactgat ccatgcatta ttcatgttta ccttgcccaa gccggattat
      361 gatctgccgc caccaagaaa gcaggaaatt cgcattgagc tggtcaaaga acagccccct
      421 gcacctgtac agccagctgc cgagccagaa ccaacgccgc ccaagccaat accggaacca
      481 cccaaaccac agccaactcc tgtcaaacag gaacagaaac ccattaaaca cgacccaata
      541 ccgcaaaaac caacgccggt accagaacct gtcgtggagg cacctcgccc agctgttgac
      601 tcacccacac cgccagtgat tgccgccaaa ccgacagtca gtgaagcgaa accggaagtg
      661 gtcgttccgc cagcaccgcc acctgaaccg gtcaaagcag gcccgagtga gggcgatata
      721 gaggcggcgc gcaatgcctt caggaatgca gcgcatcgtg agctcaagaa aaatcaacgc
      781 taccccagaa ttgcccagga tcgaggcatt gagggagatg tgaagctgag catcaatatg
      841 gacgatcatg gcaacgtcac tgggattgaa atcgtggagt ccagtggcaa caaggcgatt
      901 gatgatgcag cgatgactgc cgcgcagaaa tcccagctaa aacagcactt taccgaaata
      961 ctgcatggca aaatcaataa cattattgtc acggtcagct ttaagttgtc cagttagact
     1021 gccatgtttt tcaagcgaac atcctcctca ataaacggcg cccacgacaa gcctctcagg
     1081 tatctgctgt tatttcacga cctgtatttc attgccctga ttatcctggc cgtcgccagt
     1141 ggtggttatg gcatttacct atgggacaaa gcatctacac agtcacaacg tattaacttt
     1201 gtgatccagg aaatttacct ggtgcgcggt gacctttacc gacagactaa agagttattc
     1261 gatgctttct tcttgcagga agaaaatgca ctgcatgaat acaaccaata caccacctcc
     1321 atccttgccc atctggaacg cctgcaaaac atcgcgattg accagcagga acgcgaagca
     1381 ctcatggaga tcgagcgcaa ataccgcagt ttggtgaatg aggcaccggc tttgttctat
     1441 cgctatcaaa acaatccaaa ccgtaaagcg caaaagtcta tctaccagga tattgaaacg
     1501 ggaatattca ggcattacga agaggtttcc aaacgtgctg aatacttgct gatggtcaag
     1561 cagggtgaaa tcgaacaccg gctagatgac gccaggagca attcgatagc tgtgctctca
     1621 atgccattga tactggcctg catcctgatt atatattccc ggcgtattct aaaaaattct
     1681 atcgtcaggc cgattaatga catcatgcaa gcgaccaatg ccatcagtgc gggcaatctg
     1741 agccatcagg tgcctgaagc gggggcggaa gaactggcgg tactgtctcg agaaatcaat
     1801 aaaatggctg aagacttggc tgccagccgt gatgcactgg ttaaaagtga gaaacaggcc
     1861 gcccttggct tgctggtacc tatgctagct cacaacatcc gcaacccgct ggccagtatt
     1921 cgcgcaacag cacaggtgat tgatgacccc ttacttgata aagatacctt ggaatctatt
     1981 caaggcatta tccataccgt ggaccgcctg gagcgctgga caggcagcct gctgtcctat
     2041 ctgcatccga tcaaacccat gctcaaccag ctgttgcttt cctctatcgt gcagggcgcc
     2101 atttccacct tgcaacccaa acttgatgaa aagcagatca aagtgctgga gaatagtgcc
     2161 aacatcaagc gactgatact cactgatgaa cacctgctgg aacaaatgtt atataacctg
     2221 cttctaaatg cctcggaagc ctctcccaag ggcgcagaga tccagcttcg ttgtacactg
     2281 gaaaacaatc ttttaatgtt agtgatcatt gaccaggggc caggtatgcc gtttaaacca
     2341 gacccgcatg cactgacacc cggaccaacc accaagcggt tcggcacggg gttgggcatt
     2401 ccatttgcgt ttaaggtatg tgaagagctg ggtggcgcca tgtcttttga aagtgaagca
     2461 ggctcaggca cccgtattac catcactttg ccgcagtaat atgcctgaca cccactcaga
     2521 tgaaatttgc gttttgtgct tgcttaattt tgtattttta tcaacaatgt gattaagtga
     2581 ttgtcatgac tactatgttt aattcacctg ttccagaact ctccttaccg gccacctccg
     2641 gcctgcaatt
//
LOCUS       DATMSK010000169        11019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_12492,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000169 DATMSK010000000
VERSION     DATMSK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12492"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VNZ84_05630"
     CDS             <1..138
                     /locus_tag="VNZ84_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HWU66471.1"
                     /translation="EVPNDPWGKPYQYRAPGQHGEYDLFSLGKDGQVGGSDEAQDITN
                     W"
     gene            145..1317
                     /locus_tag="VNZ84_05635"
     CDS             145..1317
                     /locus_tag="VNZ84_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWU66472.1"
                     /translation="MHYQAKVLNGATLTMVELQADDEQDAYAQLKSKGLMPVSLHAQK
                     IAKTSRREAFPLALFSQELLALLESGLNLVEAIDALSLRQDHTRVVLQGILQSLQQGN
                     AFSAALERQPAVFPPLYIATVKAAERTGDLGNALSRYIAYDNQVSLLKKKIVSASIYP
                     LLLIVVGGLVIAFLMGFVVPKFSSVYEGTGADLPWMSKMMLHWGTLISQHGEAMLAMV
                     AGMIIGLVLLFKTPATRQSLLKAIWRIPAIGGKLHLYQLARFYRTLGMLLAGGTPIMT
                     ALGSLGGLLDSSLARSLQQAQTMISQGQPLSQAFQTVGMTTPIGERMLLVGERGGNVA
                     QMMDRIASFYDEELARWIDWFTKLFEPLLMLFIGLVIGVVVLLLYMPIFELAGQIQ"
     gene            1314..2996
                     /locus_tag="VNZ84_05640"
     CDS             1314..2996
                     /locus_tag="VNZ84_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWU66473.1"
                     /translation="MNQLLPILSAEVLAQAQTQAIQQQRRVIEVLEELAPVDNETMVS
                     QLAHVLHYPVATMREMREWQVAFDIVSYNDAQQKECLAFYDAEQHLILVFADPFNRQL
                     SLWAQHSVQVPFEWRLAHRLEISAYLAGEEETMRAMDGVGLSTEQAQEGDEEVVMQLS
                     LKSIHEDANPIVKLINSTLYDALKTGASDIHLETHPGGLAVKYRIDGVMASIANSPGL
                     DSAEQAISRIKVMAQLDIAERRIPQDGRFKVHAMGRLIDFRVSIMPSAFGEDAVLRVL
                     DRKALTEQAQGLSLKALGFDADVIARFRKLASEPYGMVLVTGPTGSGKTTSLYAAISE
                     VNTGFDKIITIEDPVEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDA
                     ETAQIAVQAALTGHLVFSTVHANSVLDVIGRFMHMGVDPYHFVSAVNGIIAQRLVRAL
                     CPQCAEPFVPDEEMIAASGLNLAQASNMQFKHAKGCGHCRGTGYKGRKAVAEMLVLND
                     ELREMIIARQPIRQLKEAAARNGMRTIREAAVSAAAEGMTSLQEINRVTFIA"
     gene            2977..3813
                     /locus_tag="VNZ84_05645"
     CDS             2977..3813
                     /locus_tag="VNZ84_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66474.1"
                     /translation="MSRLLPDLRWLGLTRLLVLSDRLWAESWPAGLRSRRVVAQLEWP
                     DSDVQPLASALQALMAKLPASRWQRIEIYLTDRHAHVLQLPAMASSLLTFSWSGEEQL
                     AYARAVLMQTYGEAARAWPLRLQDIREARDYLLATIPALQSVDLSALLTKHAVQWSVQ
                     PYVSALWAQARLPEHGTVLTAESQMLRLLQLQHGHIVHVASLAVDLFEGETITAWLMR
                     ERTLLGVQASPCYWLMEPGCDVMALTGGRLKQALSTHLTLQTLYAAHPVTTLWQEAMH
                     VA"
     gene            3803..4366
                     /locus_tag="VNZ84_05650"
     CDS             3803..4366
                     /locus_tag="VNZ84_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HWU66475.1"
                     /translation="MWPNADYQFEFAKPAFAWRAVRWPGVVLVLLLAIAVGIAYTVLT
                     EQAVQVQALVQRQQQLLAQHVTEHPAPTSAQKAPPKPAIKLSQPEQKAAEKIVQQLNV
                     RWFDLLQALEAKQVPEVALLQLLPDANRGQFVLSGEAKNYQALLKYVSQLQTVAVLEQ
                     VHLQKHQVNESHPQRPVSFEIQGGWQP"
     gene            4363..4977
                     /locus_tag="VNZ84_05655"
     CDS             4363..4977
                     /locus_tag="VNZ84_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66476.1"
                     /translation="MKQLRLPTLMTLLWLGRRCAWYIGWIPMLMSVLLLLAWVMVLQE
                     QATQEKAFDALEKEIRQLAIKPVIPQASVPTVIEEQVVTIPEPVQLAELWQQLPAFDD
                     LSPRMMQIANIAQKRNISLNVGDYQWQAHQVNAASHDIQQFDMRFTIQADYVTCRKFI
                     IEVLRKYPSMALTGLELRKNETVQPTIDATLTFSVFIRGGVHVS"
     gene            4967..5599
                     /locus_tag="VNZ84_05660"
     CDS             4967..5599
                     /locus_tag="VNZ84_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66477.1"
                     /translation="MSLDPKASTRPLVWIALVATLGLVCWQWSQEDSNPEDIVSRPAK
                     ASHVPVTTQVSRQQQLPLKVVRPVDSAAAVIPSPAPYPYFTRALPEGKVSHALFVAHE
                     WLPPPPKPQPPPPPEAPPLPYTYVGSMQDVPDGDTVILMQQKKVLLSRLGSQINTQWR
                     LDREDAQAVYFTYLPLNKPVMLSKTKTASASQRQASANAQNNYSEEMFNQ"
     gene            5659..7629
                     /locus_tag="VNZ84_05665"
     CDS             5659..7629
                     /locus_tag="VNZ84_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HWU66478.1"
                     /translation="MPSAIVLGVSVLLLSSCSLFPPKLNATTPEGKVHELAQQVAEHP
                     ADAVLRNRWFREREQAINLLLTEAEAADAKGDIATAKQVYARILTLDPHHIKALDYAR
                     ASEREIVLRKQLDQVKQHMDNPKQALKEVRDILLEQPTNTEAQALERQLIAQEPRARA
                     ALPQLQSNLSKPVTLELRDANIKVVFEALSRATGINFVLDKDIKPETKASVFVKKMPI
                     EDAIDMVLASNGLQKKVLSPASVLVYPATQQKNKDYQDLLIRNFYFANTSAKQCADML
                     RTILKVRDIYVDERLNMLVLRDRPEVLALAEKMIKAQDIADPEVMLEIEVMEITRGKL
                     NQLGILYPSSLTVLDSQLTLEALKRIKSSDIGVSPNPSVQFKTTDSDLNLLSNPRIRV
                     KNNEKAKVLVGNKVPVITTNTTANVGTSESVSYVDVGLKLEVEPRVMLDDFVSIKVNL
                     EVSSLGEAITLSNNSKVYNIGTRNATTMLRLKHGETQILAGLIQDSERKNADKLPGLG
                     EIPILGRLFSRHIDEKNKTEIVLAITPKVISNVTLPEASFSEYWSGTDTVISDKPLLN
                     SVTSSPLTRTQQMREQQLQRQRELNDATSSPVNEVPESAPAPEPVTIPAPDAQATEKE
                     PQPDSAMPGLSNNVSDIPEQGTTSLPSPKVIP"
     gene            7629..8141
                     /locus_tag="VNZ84_05670"
     CDS             7629..8141
                     /locus_tag="VNZ84_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWU66479.1"
                     /translation="MSLCRLFKRGVMRRSGFTLIELMVSLAIIAIMASVATPMIQLTV
                     KRQKEQQLREHLREMRRAIDAYKKASDDGRIKKSADASGYPPNLQVLVDGVEDAKDAK
                     HTRLRFLRRIPQDPMLNMQANNGLNQPAGWGMRSYDSPPEQPRYTQDVYDVYSLSKQT
                     GINGVPYAQW"
     gene            8128..8520
                     /locus_tag="VNZ84_05675"
     CDS             8128..8520
                     /locus_tag="VNZ84_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWU66480.1"
                     /translation="MRSGSFKCVLRGFTLVELLVVLSILALLLSLAVPRYFSGVQRAK
                     EQALKQQLTTTRKALDEYYADQGQYPETLQELVDKHYLDKLPWDPIAERNDGWVITPP
                     ELPLVGGVYTLHSNAEGQATDGSAYTDW"
     gene            8534..9022
                     /locus_tag="VNZ84_05680"
     CDS             8534..9022
                     /locus_tag="VNZ84_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWU66481.1"
                     /translation="MPKQTGFSYIGVMVTLVIAAIGLQGAAVLWQQQTQRHNETVLLE
                     TGEAYRLAIGRYYESTPQPVKQYPLRLEELLEDKRFPVPKRHLRRLYPDPFFVKQGMT
                     LIIKDGRIVGVQSQSLLEPIRHSGYQESQAGFNQAKHYREWLFTYEPNTLADLEQAWV
                     NR"
     gene            complement(9048..9716)
                     /locus_tag="VNZ84_05685"
     CDS             complement(9048..9716)
                     /locus_tag="VNZ84_05685"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWU66482.1"
                     /translation="MNTPCLRLDCIQLATLLLAMTVFCSTTYAGSLPGVEPLTEHILL
                     DNSNEQELIISNTEITATENDLLTQATDSKWQVKSDWKQKTVLYQAEVQAAAQATQLD
                     PALIHAVIATESGYNPKALSRKGAYGLMQVLPATAQTMTTIPVKQWSVRQQILWGSHY
                     LRRLLNMFEGNVTLALAAYNAGPQAVKTYHHAVPPFTETRQYVPKVLGYYQAFKTRLS
                     PMDD"
     gene            9984..10817
                     /gene="epsA"
                     /locus_tag="VNZ84_05690"
     CDS             9984..10817
                     /gene="epsA"
                     /locus_tag="VNZ84_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated transcriptional
                     regulator EpsA"
                     /protein_id="HWU66483.1"
                     /translation="MDTTITGMDQVNDQWALALNEEQRALFMEVIEESLRLQHRSHFF
                     NWLQRGFQCLLAHEVVIIGVRGLERANYDFEYLTSSRYFGDTQFDQVLNEEDGLVNEA
                     FTQWSKLGMPVFYHTELPDQQYNHYAVEHVDAERMLASELKRFVVHGFGNEHSRIATV
                     VMFGRLSSPVNAQTAHLLELLMPHLHCAIVKVTANKCPSVLSTTARNGRIKPLSKREL
                     EVLEWLQAGKTNWEIGSIMQVSPLTIKNHVQNILRKLDVENRSQAASKALKLGFITRK
                     Q"
BASE COUNT         2686 a         2691 c         2965 g         2677 t
ORIGIN      
        1 gaagtgccca atgatccctg gggcaaaccg tatcaatatc gtgcgcctgg gcaacatggc
       61 gaatatgact tgttcagctt gggtaaggat ggtcaagttg gtggcagtga cgaagcgcag
      121 gacattacta actggtagac cgccatgcac tatcaggcaa aagttcttaa cggcgccacc
      181 ctgaccatgg tagagctgca ggctgacgat gagcaggatg cttatgcaca actaaagtcc
      241 aaaggcttga tgccggtttc gctacatgcg cagaaaatag cgaaaacctc cagacgtgaa
      301 gcctttccgc tggcattgtt cagccaggag ttgctggcct tgctcgagtc tggcctgaat
      361 ctggtcgaag cgattgatgc tttgtcttta cgtcaagacc atacacgggt tgtgttgcaa
      421 ggcatcctgc aatcgctgca gcaagggaat gctttttctg cagcacttga gcgccagcca
      481 gccgtttttc caccacttta tattgccacc gttaaagccg cagagcgtac gggtgaccta
      541 ggtaatgcat tgtcacgcta tatcgcctat gacaaccagg tgtccttgct caaaaagaag
      601 attgtcagtg cctcgattta ccccctgttg ctgatcgtgg ttggtggcct ggtcattgcg
      661 tttttaatgg gatttgtcgt gccgaaattc agcagtgttt atgaagggac aggggctgat
      721 ttgccatgga tgtcaaaaat gatgttgcac tggggtacgc tgatttcaca gcatggcgag
      781 gcgatgctgg cgatggtggc aggcatgatc attggcctgg tgctattgtt taaaacacct
      841 gctacgcgcc agtcactgct caaagcgatc tggcgcatac cggcgattgg tggaaagctg
      901 catctttatc agctcgcccg tttttaccgc acattaggta tgttgttggc gggaggtaca
      961 cccatcatga cggcgctagg ttccttaggc ggcttattgg atagcagcct ggcccgcagc
     1021 ctgcagcagg cacaaaccat gatcagccaa ggtcagccat tatcgcaggc gtttcaaacc
     1081 gtgggcatga ccaccccgat tggagagcgc atgctgctgg ttggcgagcg tggcggcaac
     1141 gttgcgcaga tgatggatag aatcgccagc ttttatgacg aagagctcgc gcgctggatc
     1201 gactggttta ccaaattgtt tgagccatta ctcatgttgt ttattggcct ggtcatcggt
     1261 gtggttgtgc tcctgttata tatgcctatc tttgaactgg cagggcaaat ccaatgaacc
     1321 agctattgcc gatcttatct gccgaagtat tggcacaagc ccaaacgcag gcgatacagc
     1381 agcaacgccg cgtgattgaa gtgctcgaag agcttgcgcc cgtcgacaat gaaaccatgg
     1441 tcagccaatt ggcgcatgta ttgcactatc cggttgcgac catgcgtgag atgcgtgaat
     1501 ggcaggtcgc ctttgacatc gtaagttata acgatgcgca gcagaaagaa tgcctggcgt
     1561 tttatgatgc tgaacaacac ttgatcctgg tgtttgctga cccatttaac cgtcagttat
     1621 cactgtgggc gcagcattcg gtgcaagtgc cgtttgagtg gcggctggca caccggctgg
     1681 agatcagtgc ttacctggca ggcgaagaag aaacgatgcg tgccatggat ggggtagggt
     1741 tgtcgaccga gcaagcgcaa gaaggcgacg aagaagtcgt catgcagctg tcacttaagt
     1801 ctattcatga agatgcgaat cccatcgtca agctgattaa ctccaccttg tatgacgcgc
     1861 tgaaaaccgg cgcaagtgat atccatctgg aaacgcatcc cggtggtctg gccgtgaagt
     1921 accgcattga cggtgtcatg gccagtatcg cgaatagtcc gggtctggac agtgcagagc
     1981 aagccatttc acgtatcaaa gtcatggcgc aactggacat tgccgaacgc cgtattccac
     2041 aagatggccg ctttaaagta catgccatgg ggcggctaat tgattttcgc gtgtctatta
     2101 tgccaagtgc ctttggtgaa gatgccgtac tgcgagtact agaccgcaaa gctctgaccg
     2161 agcaggcaca gggcctgagt ttgaaagccc ttggatttga tgccgatgtg attgcccgct
     2221 tcaggaaact cgccagtgag ccttatggca tggtgctggt cactggcccg acgggtagtg
     2281 gcaaaacgac ctctctctat gctgccattt cagaggtcaa taccggtttt gacaagatta
     2341 tcaccatcga agatccagtg gaatatcaat tgcctggcgt gctacagatc cccgtcaatg
     2401 agaaaaaagg cctgacgttt gcacgcggcc tgcgatcgat tctgcggcat gacccggaca
     2461 aaattatggt gggcgagatt cgggatgctg aaacggcaca aattgccgtg caggccgcac
     2521 tcactggcca cctggtgttc tccaccgtac atgccaacag cgtgctcgat gtgattggcc
     2581 gttttatgca tatgggtgta gacccttacc actttgtgtc tgccgtgaat ggcattattg
     2641 cccagcgcct cgtacgtgcc ttgtgtccgc aatgtgccga accctttgtg ccggatgaag
     2701 aaatgattgc cgcgtctggc ctaaacttgg cacaggcctc caatatgcag ttcaagcatg
     2761 ccaagggttg cggccattgc cgtggcactg gctataaggg tcgcaaggcg gttgcggaaa
     2821 tgctggtact caacgatgag ctacgcgaga tgattatcgc aagacagcct attcgccaat
     2881 taaaagaagc ggccgccagg aatggcatgc gaaccatacg cgaagcagcc gtcagcgcag
     2941 ctgccgaagg catgaccagc ttacaggaga tcaatcgtgt cacgtttatt gcctgacctc
     3001 cgctggctag gccttacacg cctactggtc ttgtctgacc ggctgtgggc tgagtcttgg
     3061 ccagctgggc tgcgttcgcg ccgggtggtg gcgcagctag agtggcctga ttctgatgtg
     3121 cagcctttgg cttctgcttt gcaagccctg atggctaagc tccccgctag ccgttggcag
     3181 cgcatagaaa tttatctgac agacaggcat gcgcatgtac tgcaattgcc tgccatggcg
     3241 tcatccctgc tgacattcag ctggtcagga gaagaacagt tagcgtatgc ccgtgcggtg
     3301 ctgatgcaaa cttatggtga agccgcacgt gcatggcctt tgcggttgca ggatatccgt
     3361 gaagcccggg attacctgct ggcgaccatt cctgcgctgc agtctgtaga tttgtctgcg
     3421 ttgctgacga agcatgcagt gcaatggtct gtgcagcctt atgtttccgc actttgggcg
     3481 caagcacggt tacctgaaca tggcacggtg ttgactgcgg agtcacagat gttgcgactg
     3541 ctgcaattgc agcatggtca tattgtgcat gtggcaagcc tggcagttga cctgtttgag
     3601 ggggaaacga tcaccgcttg gttgatgcgt gagcgcactt tgctaggggt acaggcctct
     3661 ccatgttact ggttgatgga gcctggatgc gatgtcatgg cactgactgg tggccgcctg
     3721 aaacaggcat tgtctacaca cttaacactg caaaccttat atgcggctca tccggtaacc
     3781 accttgtggc aggaggcgat gcatgtggcc taatgccgat taccagtttg agtttgctaa
     3841 gcccgcattt gcctggcgcg cagtgcgctg gcccggtgtg gtgttagtgc tcttgcttgc
     3901 aatcgctgtg gggattgctt atacggtatt gacggagcaa gcggtgcaag tgcaagcatt
     3961 agtccaacgc cagcaacagc tattagcgca acatgtcact gaacatcctg cgccaacttc
     4021 agcgcagaag gcacctccca aacctgccat caagctcagc cagcccgagc aaaaggcggc
     4081 agaaaaaatt gtgcaacagc tcaatgtgcg ctggtttgac ttgctgcagg cacttgaggc
     4141 caagcaggtg ccagaggttg ccctgttgca attgttgcct gatgccaatc gtggtcaatt
     4201 cgtcctttct ggcgaagcga agaattatca ggcactgctc aaatatgtga gccagttgca
     4261 aactgtggct gtgcttgagc aggtgcattt acaaaaacac caggtcaacg agagccatcc
     4321 gcaacgcccg gtcagttttg aaattcaagg gggatggcaa ccatgaagca actccgcttg
     4381 ccgaccttga tgactttgct ctggctgggg cgccggtgtg cctggtacat cggctggatc
     4441 cctatgttga tgtccgtgct gttgttattg gcatgggtga tggtgttaca agaacaggcg
     4501 acacaagaaa aagcatttga tgcgctagaa aaagaaatcc ggcagttggc catcaaaccg
     4561 gtaataccgc aagcgtctgt gccgacggtg attgaagagc aagtcgttac tattccggaa
     4621 cctgtccagc tggctgagct ctggcaacaa ttgcctgcat ttgatgactt gtctccacgc
     4681 atgatgcaga ttgccaatat tgcgcaaaaa cggaacattt cactcaatgt gggcgattac
     4741 caatggcaag cgcatcaggt caacgcagcg agccatgata ttcagcaatt tgatatgcgc
     4801 tttaccatcc aggccgacta tgtcacttgc aggaaattta tcattgaggt gttgcggaaa
     4861 tatccctcca tggcattgac cgggctagag ctgcgcaaaa atgaaacggt gcaaccaacc
     4921 attgatgcca cgctgacatt ctcggtgttt atccgtggag gcgtacatgt ctcttgatcc
     4981 taaagcctct acgcggccgt tagtatggat cgccttggta gctaccctgg ggttggtgtg
     5041 ctggcaatgg tcgcaagagg atagcaaccc agaagacatc gtgtccaggc cagccaaagc
     5101 atcacacgtg cccgttacaa cgcaggtaag cagacagcag cagttacccc taaaggtggt
     5161 gcgtccagta gactccgcgg ccgcagtcat accctcgcca gcgccttatc cctatttcac
     5221 ccgggcattg cctgagggca aggtgagtca cgccttattt gttgcgcatg agtggttgcc
     5281 accaccgcct aagccacagc cgcccccgcc acctgaagca ccgccgttgc cttataccta
     5341 tgtgggtagc atgcaggatg tcccggacgg tgatacggtc attctcatgc aacagaaaaa
     5401 agtactgctc tcgaggctgg gcagccagat caatacgcaa tggcggctag accgcgaaga
     5461 tgcgcaagca gtctatttca cttatttacc gctcaataaa ccggtgatgt taagtaaaac
     5521 aaaaacagcg tcagccagcc agcgccaggc cagtgccaac gctcaaaaca attattcaga
     5581 agaaatgttt aaccaatgag tgcttcaaat atgagcttta tcatgcgggc tgctgtttca
     5641 gggaggaaca taccgtgcgt gccatctgcc atcgtgctgg gagtatcggt attactactg
     5701 tcatcctgca gtttgttccc tcctaaacta aacgcaacta cgccagaggg gaaggtgcat
     5761 gagttggcgc aacaggtggc ggaacatccg gcagatgcgg tattgcgtaa ccgctggttc
     5821 cgcgagcgtg agcaagcgat taatctactg ttgaccgagg ctgaagcagc agatgccaaa
     5881 ggcgatatcg ctactgccaa acaggtctat gcccggattc tgaccttgga cccgcaccat
     5941 atcaaagccc ttgattatgc gcgtgccagt gagcgcgaga ttgtcttgcg caaacaactt
     6001 gaccaggtca aacagcatat ggataaccct aagcaagcct tgaaagaggt cagggatatt
     6061 ttgctggagc aacctaccaa tacagaagca caggccctgg agcgacagct gatcgcacaa
     6121 gaaccgcggg cgcgtgcagc tttaccgcaa ttgcaaagca atttgagtaa acctgtcacg
     6181 ctggaattgc gagatgctaa tatcaaggtc gtctttgaag ccttatcacg tgctaccggt
     6241 atcaattttg tgctggataa agatattaaa ccggaaacca aagcttcggt atttgtgaaa
     6301 aagatgccga ttgaagatgc gattgatatg gtgcttgcga gcaacgggtt gcagaaaaaa
     6361 gtgttatcac cagccagtgt cctggtgtat ccggcgaccc agcaaaaaaa caaggattat
     6421 caggatctgc tgatccgtaa tttttatttt gccaacacca gtgccaagca atgcgccgac
     6481 atgttgcgta cgattttgaa agtccgtgat atctatgtcg acgaacgctt gaatatgctg
     6541 gtactcagag atcggccgga ggtgctggcg ctggcagaaa aaatgatcaa ggcgcaggac
     6601 attgccgacc ccgaggtcat gctggaaatc gaggtgatgg aaattacgcg cggtaaatta
     6661 aatcagttgg ggatcctcta tccctcgtca ttaacggtgc tggatagcca gttaacgctg
     6721 gaagccctta aacgtatcaa gtcgtctgat attggtgtga gccccaatcc cagtgtgcaa
     6781 ttcaaaacca ctgacagtga tttgaacctg ttatccaatc cgcgtatccg cgttaaaaac
     6841 aatgagaaag ccaaggtgct ggtaggtaac aaagtgccgg taattaccac aaatactacg
     6901 gccaatgtgg ggacttctga gagcgtgtct tatgtcgatg tgggcctcaa gctggaggtt
     6961 gagccgcgtg tgatgctgga cgattttgtc agtattaaag tgaatctgga ggtcagttca
     7021 ttgggggagg ccattaccct gagcaataac tccaaggtgt ataacattgg aacgcgcaat
     7081 gcgacgacca tgctacgcct caaacatggt gaaacccaga ttctggcggg tttgatccag
     7141 gactctgagc gcaaaaacgc tgataaatta cctggtctgg gtgaaatccc gattctggga
     7201 cgcttgttct cccgccatat tgatgaaaaa aataaaaccg aaattgtgtt ggcgatcaca
     7261 cccaaggtca ttagcaatgt gaccttgccg gaagcctctt tttcagagta ctggagcggt
     7321 accgatacgg tcatttctga taaaccgctg ctcaatagtg tgacaagcag ccccttgacg
     7381 cgcacccaac aaatgcgtga acagcagtta caacgccagc gtgaattgaa tgatgctacc
     7441 tcatcgcctg tgaatgaagt gcctgaatct gcacctgctc cagagccggt gaccatccct
     7501 gcaccagatg cacaggccac tgaaaaagag ccgcagcccg attcggcaat gcctggactg
     7561 tctaataacg tgagtgatat tccagagcag ggaacgacta gcttgccatc acccaaggtg
     7621 attccttaat gtcgttgtgt cgtttattta aacgtggtgt gatgaggcgc agtgggttca
     7681 ccctgattga gctcatggtg tcgctggcga ttattgccat tatggccagt gtggcaacac
     7741 ccatgataca gctgacagtg aaacgacaaa aagagcagca gttacgcgag catttacggg
     7801 aaatgcggcg cgcgatagat gcttataaaa aagcctcgga tgacggacgc atcaagaaaa
     7861 gtgctgatgc cagtggctac ccaccgaatt tgcaagtctt ggtggatggc gtagaagatg
     7921 ccaaggatgc caaacatacc cggttgcgat ttttgcgtcg tattccgcag gatccgatgc
     7981 tgaatatgca ggcaaataac ggtttaaacc agccggctgg ttggggcatg cgcagttatg
     8041 atagcccacc ggaacagccg cgctatacac aggatgttta cgatgtgtat tccctgagca
     8101 agcaaacagg gatcaatgga gtgccttatg cgcagtggta gcttcaagtg cgtgttacgc
     8161 gggtttacat tggttgaatt gctggtggtg ctgtccatcc tggccttatt attatcattg
     8221 gcggttccgc gttatttttc tggtgttcaa cgtgccaagg agcaggcact caaacagcag
     8281 ttaaccacaa cgcgaaaggc cttggatgag tattatgccg accagggcca gtacccggaa
     8341 acactgcaag agttagttga taaacattat ctggacaaat tgccttggga cccgatagcc
     8401 gagcgtaatg atggttgggt cattacccca ccggaattgc cactggtagg gggcgtgtat
     8461 accttgcaca gtaacgcaga agggcaagcc acagatggtt cagcgtatac cgactggtag
     8521 ggcttattgt gacatgccca aacaaacggg gttttcttac ataggcgtca tggtgacgct
     8581 agtgattgcg gcgattggtt tgcagggtgc tgccgtccta tggcaacagc aaacccagcg
     8641 tcataatgag acggtattgc tggaaacggg tgaagcctac cgcctggcca ttggccgtta
     8701 ttatgaatcc acaccacagc cggttaaaca atacccgcta cgcctggaag aactgttgga
     8761 agataagcgt ttcccggtcc ccaaacggca tttacgtcgg ctctatccag accccttttt
     8821 tgtaaaacag ggcatgacct tgatcatcaa ggatggacgt attgtcggcg tgcagagcca
     8881 gtccctgctt gaaccgatcc gccatagtgg gtatcaggag tcgcaggcag gatttaatca
     8941 ggcgaagcat taccgcgagt ggctgtttac ctatgagcca aacaccttgg cagatttgga
     9001 acaggcctgg gtcaatcggt aaccaagtgg gtgatccatc gagacagcta atcgtccata
     9061 gggcttaagc gggttttaaa cgcttgataa tagccaagca ccttaggcac atattgccgg
     9121 gtttcggtaa aaggcggcac tgcatgatga tatgtcttaa cggcttgcgg gccggcatta
     9181 taggcggcca gtgccagtgt gacgtttcct tcaaacatat tcagcaaccg ccttaaatag
     9241 tgtgatcccc ataatatctg ttggcgcaca gaccattgtt tgacggggat ggtggtcatg
     9301 gtttgcgccg ttgccgggag aacctgcatc agcccataag cgcctttgcg tgataaggct
     9361 ttgggattgt agccagattc ggtggcaatg acggcatgga ttagtgccgg gtctagctgg
     9421 gtggcttgtg cggctgcttg cacttctgcc tgatagagga ctgtcttttg cttccaatcc
     9481 gacttcactt gccacttgct atcagttgcc tgcgtcaata gatcattttc agtggcagtt
     9541 atctccgtat tgctgataat gagctcttgc tcattggagt tatccagcag gatatgctcg
     9601 gtgagcggtt ctacacctgg cagactgcct gcgtaggttg tactgcaaaa aaccgtcatt
     9661 gccagcaata atgtggctag ctgaatgcaa tccaaacgca ggcagggagt attcataatg
     9721 gaaggattgt gccatgccta tatgacatgg caaagacaat caggtctagg ctaggcctcc
     9781 gattttatga cgtattcgct gggtgttcat gcttgctgca atgctgtttg agccacaagt
     9841 cattcgtagt ctattggctt cttcgcatag tcttgatcac taatccgttg tcacgaattt
     9901 atcaatcaac tgacacgccc gaatcatttt tctgtcacaa attctgagca aacttgctgc
     9961 aattatataa atgtgcaaaa cacatggata ccacgataac agggatggat caggtcaacg
    10021 accagtgggc gttggcactg aatgaagagc aacgcgcgct ttttatggaa gtgattgagg
    10081 agtctctgcg tctgcagcat cgctcgcact tctttaactg gctacaacgg ggatttcagt
    10141 gtttattggc ccacgaagtg gtgatcatag gtgtacgtgg cctggaacgc gccaattatg
    10201 attttgaata tctgacctcc agccggtatt ttggcgatac tcaatttgac caagtgctca
    10261 atgaagagga tggcctggtc aatgaggcat ttacgcagtg gagcaagcta ggcatgccgg
    10321 tgttttacca tactgagctc cctgaccagc aatataacca ctatgccgtt gaacacgtgg
    10381 atgctgagcg tatgctggct tctgaactta aacgttttgt ggtgcacgga tttggtaatg
    10441 agcatagccg cattgcaacc gtagtcatgt ttggccggtt aagctcacca gtgaatgcgc
    10501 aaacagcgca tctgctcgaa ttgctgatgc cacacctgca ttgcgccatc gttaaagtga
    10561 ctgccaataa atgcccgtct gtgttgtcca caaccgcccg taatggacgt atcaaacctt
    10621 taagcaagcg cgagctagaa gtgctggaat ggttgcaagc cggtaaaacc aattgggaaa
    10681 tcggcagcat aatgcaggtc agcccattaa cgatcaaaaa ccatgtacaa aacatcttgc
    10741 gcaagctgga cgtggaaaac cgcagccagg ccgccagcaa agccctcaag ctgggtttta
    10801 ttacccgcaa gcagtaaacc tctccaattt ttctcctgtt caatcggcac tttatccaac
    10861 tagtccgttt ggactagtgg cttcctcttc ccctgtcatt tttatttcct agactgctcc
    10921 taatttttat gtgggcatct tgctcagggc ggactagtcc atatggacta ggtttgcaaa
    10981 gtaagcctgt catgttgccc ccccataatt tttcgggat
//
LOCUS       DATMSK010000170         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_388863,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000170 DATMSK010000000
VERSION     DATMSK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388863"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..896
                     /gene="truA"
                     /locus_tag="VNZ84_05695"
     CDS             123..896
                     /gene="truA"
                     /locus_tag="VNZ84_05695"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWU66484.1"
                     /translation="MKIAIGIEYHGAYFQGWQSQPNAAGVQDALERAIAQVGGESVRL
                     HAAGRTDSGVHALMQVAHYETSVPRPLNAWVRGTNAHLPAWVRVLWASEVADDFHARF
                     SARARSYQYVLFNHAVAPASQHGRVGWYHRPLNIDLMQQAIQILVGEHDFSAFRASEC
                     QASSPIRTMRRAEIVQHGRYFIFSFKANAFLQHQIRNMVGALVYVGHGKLSVTGFAEL
                     LEHKDRRLSPPTFMPDGLYFTGVDYDASWQLPLCEARWD"
     gene            913..1560
                     /locus_tag="VNZ84_05700"
     CDS             913..1560
                     /locus_tag="VNZ84_05700"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWU66485.1"
                     /translation="MIRTRVKICGITRLQDASTAIDAGADALGFVFYAPSPRAVTAAA
                     AQAITAVLPPFVSKVGLFVNAEAREVQEAIATAGLDCLQFHGDESADYCTQFNLPYYK
                     AIRVKPGVNLVQCELDFASASALLLDTYSEKAVGGTGEAFDWSLIPAGLQKPLVLAGG
                     LNPENVVQAINQVRPYAVDVSGGVEAEKGIKSPQKIAAFMQQVMQCDAARNGRLE"
     gene            1583..2785
                     /gene="trpB"
                     /locus_tag="VNZ84_05705"
     CDS             1583..2785
                     /gene="trpB"
                     /locus_tag="VNZ84_05705"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWU66486.1"
                     /translation="MQVYDMPDERGHFGPFGGVFVAETLVEALEELRVMYEKYRHDPE
                     FLAEFAYDLKHFVGRPSPIYHAKRLSDKVGGAQIYLKREDLNHTGAHKINNTIGQALL
                     AKRMGKPRVIAETGAGQHGVATATIAARLGLECVVYMGAEDVKRQAPNVFRMKLLGAT
                     VVPVESGSKTLKDALNEAMRDWVTNISNTFYIIGTVAGPHPYPMMVRDFQAVIGIEAK
                     EQMQEMIGRQPDAVVACVGGGSNAMGIFYPYIDVEGVRLIGVEAGGHGLSTGQHAAPL
                     TANSPIGVLHGNRTYLMQDEDGNIIETHSISAGLDYPGVGPEHAWLKDIKRAEYVAIT
                     DDEAMAAFHNLCRTEGIIPAMESSHALAHAEKLAKNMSPDKVILVNLSGRGDKDINTV
                     ARLANITL"
     gene            2875..3675
                     /gene="trpA"
                     /locus_tag="VNZ84_05710"
     CDS             2875..3675
                     /gene="trpA"
                     /locus_tag="VNZ84_05710"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWU66487.1"
                     /translation="MSRIQSVFSALKAQGKKALIPYITAGDPHPDQTVPLMHALVASG
                     ADMIELGVPFSDPMADGPVIQRASERALVHKMGLRKVLEMVKAFRETNQATPIVLMGY
                     ANPIEAMGSDKFVALAHEAGVDGVLTVDYPPEECETFNQKLAAANIDSIFLLSPTTEP
                     SRTDLIVKQATGFLYYVSLKGVTGAANLDIVEVKKRVAEIRKQTDLPIGVGFGVKDAA
                     TAREVAAIADAVVVGSRMVLAIEGSDTNNLMSNVQSLMKELRTAIDSV"
BASE COUNT          900 a          821 c         1047 g          967 t
ORIGIN      
        1 agcgacagca ggccaacgag ctgcttgcaa aaatcgctta aaacaaggcc gggtctcccg
       61 gccttgtttg ttttgtgcat gccgattagc ctttatcact gactgccttt atactaggcg
      121 ccatgaaaat tgcgattggc attgaatatc acggcgccta ttttcagggg tggcagagtc
      181 agccgaatgc cgcaggtgtt caggatgccc tggaacgggc aatcgcgcag gttggtggtg
      241 agtccgttcg actgcatgct gctggacgaa ccgattcagg tgtgcatgcc ttgatgcaag
      301 tggcacatta tgaaacttcg gtcccccggc ctttgaatgc atgggtgcgt ggcaccaatg
      361 cgcatttgcc tgcttgggta agggtgttat gggctagcga agtggctgac gattttcatg
      421 cccgatttag cgctcgcgcc cgcagttatc aatacgtact gtttaaccat gcagtggcgc
      481 cagccagcca gcatggccgt gtcggctggt atcatcgacc actcaatatt gacctgatgc
      541 agcaggcgat tcaaatactg gtaggcgagc atgatttcag tgctttccgt gccagtgagt
      601 gccaggccag tagcccgata cgtactatgc gacgtgccga gattgttcag catggcaggt
      661 actttatttt cagtttcaag gccaatgcgt ttctgcagca ccagattcgc aacatggttg
      721 gtgccctggt ttatgtgggg catggcaagc tgagtgtcac tgggtttgct gagttgcttg
      781 agcataagga caggcggctt tctccaccca cctttatgcc agatggttta tattttaccg
      841 gtgttgatta tgacgcaagc tggcaattgc cgctttgtga agctcgctgg gattaaggaa
      901 aaaatacgta acatgataag aacgcgggtt aaaatttgcg gtatcacgcg attgcaagat
      961 gcatcgacag cgatagatgc aggtgcagat gcgttaggat ttgtttttta tgcaccgagt
     1021 ccgcgggcgg tgacagcggc agcagcgcaa gcaatcacgg ccgtgttgcc accttttgtc
     1081 tctaaggtgg gtttatttgt aaatgctgaa gcccgtgaag tacaagaggc tattgctacc
     1141 gccggcctgg attgtttgca gtttcatggg gatgaatcag cggactattg tacgcaattt
     1201 aatctgccat attacaaagc aatccgcgtc aaaccggggg taaatttggt acaatgcgag
     1261 cttgattttg catcggcttc agccttgctg cttgacacat attcagaaaa ggcagtgggc
     1321 ggaacaggcg aagcatttga ctggtcgctc attcctgcgg gtttacaaaa accattggtg
     1381 ttagcaggcg gattaaatcc agagaatgtg gttcaggcga taaaccaggt gcgtccctat
     1441 gcggtggatg tcagtggtgg tgttgaagca gaaaagggga ttaaatcccc gcaaaaaata
     1501 gctgcattca tgcagcaggt gatgcagtgt gatgctgcac gtaatgggcg gttagagtaa
     1561 caaatgagat acggagtaac gcatgcaagt ctatgacatg ccagatgaac gcggtcattt
     1621 tggtcctttt ggtggcgttt ttgtcgcaga aaccttggta gaagccctag aagagctgcg
     1681 ggtgatgtat gaaaaatatc gccatgatcc tgagtttttg gctgagtttg cttatgatct
     1741 taagcatttt gttggccgtc ccagcccgat ttaccatgcc aagcgccttt ctgacaaagt
     1801 gggtggagca caaatctacc tcaagcgcga agatttaaat cataccggtg cgcacaagat
     1861 caacaacacc attggtcagg cattgctggc aaagcgtatg ggcaagccac gtgtgattgc
     1921 cgagactggt gctggccaac atggcgtggc cactgcgacg attgctgccc gtctaggttt
     1981 ggagtgtgtg gtgtacatgg gcgctgagga tgttaagcgc caggcaccga atgtgttccg
     2041 tatgaagctg cttggtgcaa ccgttgtgcc ggttgaaagc ggctccaaga cgctgaaaga
     2101 cgccttgaat gaggccatgc gcgattgggt gaccaatatt tctaatactt tttatatcat
     2161 tggcacagtg gcaggtccgc acccttatcc gatgatggtg cgtgattttc aggcagtcat
     2221 tggtattgaa gctaaagagc aaatgcaaga aatgataggc cgccagcccg atgccgtggt
     2281 ggcctgtgtg ggcggtggtt cgaacgccat gggtattttt tacccgtaca tcgatgtaga
     2341 aggtgtgcgc ctgattggtg ttgaggcggg cgggcacggg ctgagcaccg gccagcatgc
     2401 tgcgccattg accgcaaata gcccgatcgg tgtgctgcat ggtaaccgca cttacctgat
     2461 gcaagacgaa gatggcaaca ttattgaaac ccattccatt tcagctggcc tggattaccc
     2521 tggcgtaggt cccgaacatg cttggttgaa agatatcaag cgagcagaat acgttgcgat
     2581 cacagacgat gaggcgatgg ctgcttttca taatctctgc cgtacagaag ggattattcc
     2641 tgctatggag tccagtcatg cgttggcaca tgccgagaaa ctggcaaaaa acatgtcacc
     2701 tgataaagta attctggtga acctgtctgg ccgtggggat aaagatatta atacggtggc
     2761 gcgcttggcc aatattacac tctgatgctg atttgcttgc cgatctgatg attaatttca
     2821 tcgggttggc aaagcaagaa acgtcattat ttgaattcat taaaaaacat tcttatgtca
     2881 cgcattcaat ctgtattttc tgcactcaaa gcgcaaggta aaaaggcact gattccctac
     2941 attacagccg gcgatcctca tcctgatcaa accgtaccgc tcatgcatgc cctggtagcg
     3001 tctggggcag atatgattga gcttggtgtc cctttttctg acccgatggc tgatgggcct
     3061 gtgatccagc gcgccagtga gcgcgccttg gtacacaaaa tgggcttgcg caaggtgctg
     3121 gaaatggtaa aagcctttag ggaaaccaat caggcaaccc ctattgtgtt aatggggtat
     3181 gccaatccga ttgaggcgat ggggagtgac aaatttgtag ccttggctca tgaagcaggc
     3241 gtggatggcg tcctgacagt ggattatccg ccggaagagt gtgaaacttt taaccagaaa
     3301 ttagcagcag ccaatattga cagcatcttc ctgttatcac ctacaactga gccatcccgc
     3361 acagatttaa ttgtcaagca ggcgactgga ttcctttact acgtttccct caagggtgtg
     3421 actggtgctg cgaacctgga tattgtcgag gttaaaaaac gtgtggccga gatccgcaag
     3481 caaactgatt tgccaattgg tgtgggtttt ggtgttaaag atgcagcgac cgcccgtgaa
     3541 gtagctgcaa ttgctgatgc ggttgtcgtg ggtagccgta tggtgctggc cattgaaggt
     3601 tcagatacca ataacttgat gagcaatgtg cagtcattaa tgaaagagtt gcgtactgcg
     3661 attgattcgg tataggccag ttttaagata aggcacctcg tcgcactatt taagcaggag
     3721 agattatgag ttggt
//
LOCUS       DATMSK010000171         6414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_294875,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000171 DATMSK010000000
VERSION     DATMSK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294875"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /gene="yedA"
                     /locus_tag="VNZ84_05715"
     CDS             complement(<1..871)
                     /gene="yedA"
                     /locus_tag="VNZ84_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HWU66488.1"
                     /translation="MAQKNHWWLVIIALSCTYFIWGSTYLAIKYAIESFPPFIMVGIR
                     FTVAGTLLYTILRSMGHAAPTWMQWRGAALVGLLLPVLGNGTVSHVQLHVSSSVAALA
                     IATAPIWMAVFSSLWGHKISRREWLGIGIGLVGIVLLNTRGSLQGDWLSAGLLMFAAA
                     SWSLGSVWSKHLAMPSGLMGAASQMLCGGLISLLFSVAFDEHLPAHISAHSWAAISFL
                     IVPGSIIAFSAYHWLLHHVRPLVASSNTFVNPVVAFAVGVWFASEQIHVMEYIALAVI
                     LVGVFLVLTATTEA"
     gene            complement(953..2485)
                     /locus_tag="VNZ84_05720"
     CDS             complement(953..2485)
                     /locus_tag="VNZ84_05720"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWU66489.1"
                     /translation="MEQIIIFDTTLRDGEQSPGAAMTKEEKIRIARQLEKLGVDVIEA
                     GFAAASPGDFDAISEIAKVIKTSTVCSLARASENDVRRAGEAIQHAAKGRIHTFIATS
                     KIHMENKLRMSEDQVVERAVQAVKWAREYTQDVEFSAEDAVRSEIDFLVRVFEAVIEA
                     GAKTINVPDTVGYSIPSVWGERMAELIKRVKNSDKVVWSTHCHNDLGMAVANSLSAVM
                     NGARQVECTINGLGERAGNASLEEIVMAIKTRSDIFNLTTRIDTTQIVPASKLVSTIT
                     GYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWGANKLTLGKLSGRN
                     AFRTRLKELGIELESEDALNAAFARFKELADKKSEIFDEDLHALVSDEVVQDTAELFK
                     LSYLQVASQTGEIPHAILTLGVDGAEQRTEAQGGGPVDATFKAIENVVQSESELLLYS
                     VNNITSGTDAQGEVTVRLAKGGRIVNGQGADTDIVVASAKAYLNALNKLYAKPEKLNP
                     QV"
     gene            complement(2622..3410)
                     /gene="pssA"
                     /locus_tag="VNZ84_05725"
     CDS             complement(2622..3410)
                     /gene="pssA"
                     /locus_tag="VNZ84_05725"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HWU66490.1"
                     /translation="MSEPQTRLSRREEHTSLRQRGIYLLPNLFTSAALFGGFYAIVQA
                     MNQHFEQSAIAIFIAMVMDGLDGRVARMTKTQSAFGAEYDSLSDMVSFGVAPALIMYV
                     WALQPMNKLGWIAAFIYCACAALRLARFNTKLDDPFQDKRFFQGLPSPAAAALLAGFV
                     WVSYEYNIDGREIFFGALQVKWIAWFLTIFAAGSMVSDLKFYSGKDINLRHSVPFVAI
                     LAVVMAFVLISYSPPEVLFSVVFVYALSGYYMWVRGRLEAKKSA"
     gene            3566..4756
                     /gene="tgt"
                     /locus_tag="VNZ84_05730"
     CDS             3566..4756
                     /gene="tgt"
                     /locus_tag="VNZ84_05730"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWU66491.1"
                     /translation="MQFSLHNTDGLARRGTVSLPHGEVQTPAFMPVGTYGTVKAMSPQ
                     ELNDIQAHIVLGNTFHLWLRPGLDVIAAHEGLHKFMGWSNPILTDSGGFQVWSLGDLR
                     KITEEGVKFQSPINGDRCFLTPEESMRIQRVLNSDIVMIFDECTPYPASHDQARRSME
                     LSLRWAQRSRDAFNVYQTQTIAQTLTMLGGPQEVSQLRSEAVPNNGNALFGIIQGGMY
                     EDLRDISRNGLEAIGFDGYAIGGLSVGEPKEDMLRILAHTAPQMPKDKPRYLMGVGTP
                     EDLVDAVSYGIDMFDCVMPTRNARNGWLFTQYGDVKIKNASYKTDTRPLDEECECYTC
                     QNFSRAYLHHLHRIGEILGARLNTIHNLHYYQVLMQGMRAAIEENRFEAFKAEFARKR
                     AEKR"
     gene            5694..6008
                     /gene="yajC"
                     /locus_tag="VNZ84_05735"
     CDS             5694..6008
                     /gene="yajC"
                     /locus_tag="VNZ84_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWU66492.1"
                     /translation="MFISNAYAAAAPATDIMSFLPMVVIFVLFYFMLIRPQMKQAKQH
                     KSMLETLKAGDEVATTGGIVGKVTKVSEHFVSIEISANTVVNVQKHTVQTLLPPGTLK
                     SI"
     gene            6067..>6414
                     /locus_tag="VNZ84_05740"
     CDS             6067..>6414
                     /locus_tag="VNZ84_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWU66493.1"
                     /translation="MNRYPIWKFVLIGTVFIIGLLYTIPNFFGESPAVQISPAKTSLK
                     LDESLLQKVESTLKLANIPFDGLFMDASGVKVRFANTDTQLLAKDKLVANLGKDYTVA
                     LNLVPQTPNWLQSI"
BASE COUNT         1698 a         1784 c         1481 g         1451 t
ORIGIN      
        1 cggcttcagt agtcgccgtt aataccagaa acacgccgac caggatcaca gccagcgcga
       61 tatattccat gacatgaatc tgctcactgg caaaccagac gccaactgca aacgccacca
      121 ccggattcac aaaagtattg ctgctggcga ccagagggcg cacatgatgc aacagccaat
      181 ggtaagccga aaaggcaata atggagccgg gcacaattaa aaagctaatc gcggcccatg
      241 agtgtgccga aatgtgggca ggcaaatgct catcaaatgc gacactgaat aataacgaga
      301 tcaggccgcc gcataacatc tggctggcag cccccattaa gccgcttggc atggccaaat
      361 gtttgctcca cacggagcct agcgaccacg acgcagctgc aaacatcagt aaaccggcgc
      421 tgagccagtc gccttgcaaa ctgccacgcg tattcagcaa tacaatgccc accaggccaa
      481 tgccaatccc cagccactca cgacgactga tcttgtgccc ccacaggctc gaaaaaacag
      541 ccatccaaat gggggccgtg gcaattgcca aggctgccac actggacgac acatgcaact
      601 gcacatgcga gacggtcccg ttacccaaca ccggcagcaa taaccccacc aaagctgcac
      661 cacgccattg catccaggtg ggtgccgcat gccccatgct acgcaagatc gtgtaaagca
      721 aggtgcctgc cacggtaaac cggatgccaa ccatgataaa aggcgggaaa ctttcaatcg
      781 catatttgat ggcaagataa gtggatcccc aaatgaaata ggtacagctc aacgcaatga
      841 tcaccagcca ccaatgattt ttttgcgcca tgccgaggcc cttacctttc atttcaccca
      901 aaataaaacg cttgctgttt gactccacag caagcgttcg cccataccgt gattacactt
      961 gaggattcag tttttccggt ttggcataca acttattcaa tgcattcaaa taagccttgg
     1021 cagaagcaac cacaatatct gtatctgccc cctgcccgtt cacaatacgg ccacctttag
     1081 ccaggcgcac tgtgacttcg ccttgcgcat ccgtgccact cgtgatgttg ttcacagagt
     1141 acagcaacag ctcagactca ctttgcacca cgttttcaat cgctttaaag gttgcatcca
     1201 caggaccgcc accttgcgcc tccgtacgct gctcggcacc atccacgcca agggttaaga
     1261 tcgcatgcgg aatttccccg gtttgtgatg cgacttgcag gtaagacagc ttgaacagtt
     1321 cggcagtatc ctgcaccact tcatcactga ccagtgcatg caagtcctca tcaaaaatct
     1381 cggacttttt atccgccagc tctttaaacc gggcaaaggc tgcattgagc gcatcttcac
     1441 tctctagctc aatccccagt tctttcaagc gggtgcgaaa cgcattacgg ccggacagct
     1501 tacccaaggt gagtttattg gcgccccagc ccacatcttc ggcacgcatg atttcatagg
     1561 tttcgcgatg cttaagcacg ccatcctgat gaatgccaga ctcatgcgcg aatgcattgg
     1621 caccgacaat cgccttgttt ggttgtacgg gataaccagt aatcgtcgac accagcttgg
     1681 aagcaggcac aatttgcgta gtatcaatac gcgtcgtcag attgaaaata tcactgcggg
     1741 ttttaatcgc catgacgatc tcttcaaggc tggcattacc cgcacgctca cccaaaccat
     1801 taatcgtaca ctcgacctga cgcgcaccgt tcatcacggc cgacagtgag ttggccacgg
     1861 ccattccaag gtcattgtgg caatgcgtgg accagactac cttatcagaa tttttgacgc
     1921 gcttgatcag ctcggccatg cgctcacccc aaacagaagg aatgctataa ccaaccgtat
     1981 caggcacatt gatggttttg gccccagctt caatcacggc ttcaaacacg cgtaccagga
     2041 aatcgatttc tgaacgtact gcgtcttccg cagaaaactc cacatcttgc gtatattcgc
     2101 gtgcccactt cacggcctgc acggcgcgct cgaccacctg gtcttcgctc atgcgcagtt
     2161 tgttttccat atggattttg ctggtcgcga taaaggtatg tatgcggcct ttggccgcat
     2221 gttgaattgc ctcacctgcg cggcgtacgt cgttttcgct ggcgcgggcc agtgagcaca
     2281 cggtggaggt tttaatcact ttggcaattt cagagatcgc gtcaaaatcg ccagggctgg
     2341 cggctgcaaa accagcttca atcacgtcca cacccagttt ttccagctga cgtgcaatac
     2401 ggattttttc ttctttggtc atcgctgcgc cgggactttg ttcaccatca cgtagcgtag
     2461 tatcaaaaat aataatttgt tccatgactg ttcctaatct gataggcggg aattttactc
     2521 gcttgtccta cacttgacca tcaccttaaa tgttttaacc agttgatacg acgcataaaa
     2581 aacgcgcctt tgaggcgcgt tttcaatgaa ccgacaatgc atcaggcaga tttttttgcc
     2641 tctagcctgc cgcgcaccca catgtagtat ccagacaagg catagacaaa taccacgctg
     2701 aacaacacct caggcggact atatgaaatc aacacaaatg ccatgaccac cgccaggatc
     2761 gccacgaacg gcactgagtg cctgagattg atatccttgc cgctgtagaa cttgagatca
     2821 gacaccatgg aaccggcagc gaatatggtc aaaaaccagg caatccactt cacctgcaag
     2881 gcaccaaaga aaatttcacg gccatcaata ttgtattcat aagacaccca gacaaagccg
     2941 gccagcaagg cagcagcggc cgggctgggc aagccctgga aaaagcgttt atcctggaac
     3001 gggtcatcaa gcttggtgtt aaagcgggcc aagcgcaacg ctgcacaggc acaataaata
     3061 aaggcagcaa tccagcctaa tttattcatg ggctgcaatg cccatacata cataatcaag
     3121 gctggagcca ccccaaacga caccatgtca gacaaactgt catactccgc accaaaagca
     3181 ctctgcgttt tggtcatacg tgccacacgg ccatctagtc cgtccatcac catcgcaata
     3241 aaaatcgcaa tcgccgactg ctcaaaatgc tggttcatcg cctgaacaat ggcataaaaa
     3301 ccaccgaata aggcagcaga agtaaataaa ttcgggagca gataaatgcc acgctgacgc
     3361 aagctggtgt gttcttcacg tcgtgatagg cgggtttgtg gttcagacat aagaaagatc
     3421 aagaatagag atgtgtacat tgttgcgggc aatggcaaaa aatgcaagca tggccggaga
     3481 gtccaaaagc ctggtcgcag catcaggagc caccctcacg ctgtgctaaa atattggttt
     3541 atttcagcta tttatcgtca tcttcatgca gttttcctta cacaataccg atggcctcgc
     3601 ccggcgcggc accgtcagcc tgcctcatgg cgaagtacaa accccggcgt ttatgcctgt
     3661 cggcacttat ggcacggtca aggccatgtc cccgcaagaa ctcaatgata tccaggcaca
     3721 tatcgtcctt ggtaacacct ttcatttatg gctgcgccct gggttggacg tgattgcagc
     3781 gcatgaaggc ctgcataaat tcatgggctg gagcaatccc attctgacgg attcaggcgg
     3841 cttccaggta tggagcctgg gagacttgcg taaaatcacc gaagaaggtg tgaaattcca
     3901 gtcacccatc aatggtgacc gctgcttttt aacgccggaa gaatccatgc gcattcagcg
     3961 cgtgctcaac agtgacatcg tcatgatttt cgacgaatgc actccctacc cagccagcca
     4021 cgaccaggca cgcaggtcta tggaactgag cctgcgctgg gcgcaacgta gccgggatgc
     4081 atttaatgtg taccaaaccc agacgattgc gcaaacactg accatgctcg gcggcccaca
     4141 agaagtgagt caacttcgct cagaagctgt gccgaacaac ggcaacgcac tctttggcat
     4201 tatccaaggc ggcatgtacg aagaccttcg tgatatttcg cgcaatggcc tggaagcgat
     4261 tggcttcgat ggctacgcta tcggtggttt atctgtgggc gaacccaagg aagacatgct
     4321 acgcattttg gcgcacactg caccgcaaat gcctaaagac aaaccacgct acctcatggg
     4381 cgtcggcaca cccgaagact tggttgatgc tgtcagctat ggcattgata tgtttgattg
     4441 cgtgatgcca acccgcaacg cacgtaatgg ctggctattc acgcagtatg gggatgtcaa
     4501 aatcaagaat gccagctata aaacggatac ccgccccctg gatgaagaat gcgaatgcta
     4561 tacctgccag aacttcagcc gcgcttattt gcatcacttg caccgcatag gcgaaattct
     4621 tggggcgcgc ttgaatacca tacataacct gcattattac caggtgctga tgcagggaat
     4681 gcgggcggcg attgaagaga accgttttga ggcgtttaag gcggaatttg ctcggaagcg
     4741 ggcggagaaa cgttaacgcc gcaatctggc gcatgagtga gtcgcaacaa ggcattattt
     4801 agaacaaagg tcactctcat tacaacactc tatttcgcct ctcggcgagt tactttctgg
     4861 tgcttgtccc ccagaaagta accaaagagg acgacaccca gccatcacga ccttcggctc
     4921 ccttgcgttg ctcaacaaag taggcgtcca tcgaaactcg ccctagcggc ttgcacactt
     4981 cgcaagccac cgcggaactc gaacagccga tggccgactt cccccacttc gtctccgcta
     5041 ctcagcgtga tgggatggga ttcatctcgc caaactcaca gcgtagaagt ttggacattt
     5101 aaaaactcaa attcttttct ttaatccaca aacggtagcg ctaatgcgtt ttcgggcccc
     5161 tatctgccgc gccgagtagc gcaggcaaaa ttagatgaag cgagcggctg ttcgaatccc
     5221 gcggtggcct gcgttttgtg caggccgcta ggatgagttt cgcgagcgct tattttgacg
     5281 agcaacgcag gataaaagcg ggagctaggg tgcactttct tttggttact tattctttgg
     5341 gcaagcaaag aaaagtaact cgccgaaggg cgaaaaagaa catcaaagac aacaagaaac
     5401 catccgcgtg ggtgtacatg cccaccctac cgctgaacta agtattcacg ctacctgaat
     5461 cctgcctctg ccaaaatgac gacttgttgc agggtacgct ggttctacga aagccagctg
     5521 ttccgtcggt gcagggcatg gtctatcccc tgctagacct tacgccggac agccttcacc
     5581 cctttttagc aaactacttc catacaccct cgaatcacaa cccgccattt attcttcccc
     5641 cgcctgtgct agaatcccgt gttatttctt agattgactc aggaacaaag tatatgttta
     5701 tcagcaacgc atacgcagct gccgctccag ccaccgacat catgagcttt ttgcctatgg
     5761 tggtgatttt tgtcttgttt tacttcatgt tgatccgccc gcaaatgaag caggccaaac
     5821 agcataaatc catgctggaa acactgaaag ccggagatga agtggcgacc accggtggta
     5881 tcgtgggtaa agtgacaaaa gtaagtgagc actttgtcag cattgaaatt tcagccaata
     5941 ctgtggtcaa tgtacaaaaa catactgtac aaactctgct gcctccaggc actttgaaat
     6001 ccatttaacc tgaccgttta aataaaccaa gacccgcgag tctttcattc tgaccaagag
     6061 tttcttatga accgttatcc gatatggaaa ttcgtgctga tcggcacagt gtttatcatt
     6121 ggcctgctgt acacgatccc aaactttttt ggtgaatcgc cagcagtaca aatcagcccg
     6181 gccaaaacct ctcttaagct agatgagagc ctgttacaaa aagtggaaag cacgctgaaa
     6241 ctggccaaca ttccatttga cggtctgttt atggatgcca gcggcgtgaa agtacgcttc
     6301 gccaataccg atacccagtt actggcaaaa gacaaactgg tcgccaatct gggcaaagac
     6361 tacaccgttg ctctcaactt ggtgccacaa acgcctaact ggttgcaaag catc
//
LOCUS       DATMSK010000172         7928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_435446,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000172 DATMSK010000000
VERSION     DATMSK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435446"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VNZ84_05745"
     CDS             complement(<1..169)
                     /locus_tag="VNZ84_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU66494.1"
                     /translation="MDLQLVGKTALVSGSTAGIGLAIASQLAQEGAQVIINGRSESAV
                     AQVVAAVAKQTG"
     gene            complement(216..1151)
                     /locus_tag="VNZ84_05750"
     CDS             complement(216..1151)
                     /locus_tag="VNZ84_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU66495.1"
                     /translation="MTTSPNHTVLIVDDVPENLAYLHDALDESGYQVYVANSGEMALQ
                     VAHLMQPHVILMDAMMPGLDGFATCRRIKAAAETQHIPVIFMTGLTEVEHVTMAFDAG
                     GVDYVTKPVRPAEVLARLKVHVQNATKMHQATSALDAFGQATIAILPTDKRLVWQTPL
                     AKQYLEQYFAEWAAKEAYSAVQTLPPMIYLWLESCIQQFSSGQSMQTLNVNQGNARLS
                     LTVANTSEEQWVVVLREYSDEAQIESLGKLFQLTKRESEVLYWTVKGKTNKDVGEILG
                     TSPRTINKHLEHVFQKLGVETRTAAASLVASKIKF"
     gene            complement(1148..4591)
                     /locus_tag="VNZ84_05755"
     CDS             complement(1148..4591)
                     /locus_tag="VNZ84_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU66496.1"
                     /translation="MSTPSTSGLSTEIRAEPIQRIVKIRRDYNNWVANETIEDYALRY
                     TPRAFRKWSILRVSNTALGAISFLVLEAIGGTITINYGFPNAMWAILAVGLMIFLTGL
                     PISYYAAKYGLDMDLLTRGAGFGYIGSTISSFVYASFTFILLSLEAAIMGYALELYFH
                     LPLYLGYLVSALIVIPLVTHGVTLISRIQMITQPIWIALMFLPFLAILKMDPGIFDRL
                     SHFAGISANGGEFNWMLFGAATAVGVALIPQIGEQVDFLRFMPERNKENYWRWNLGVL
                     MAGPGWVILGMLKMFAGALLAYMVFNRDMPLSSAINPTHMYWVGFGHVFSNPEVALAV
                     TALFVCVSQIKVNMTNAYAGSLAWSNFFARLTHSHPGRIVWVAFNVMIAIVLMELNVF
                     QALEEILGLYANIAIAWIAAVVADLVINKPLGLSPKGIEFRRAYLYDINPVGVGAMFM
                     ASALSIVAYTGAMGPLAQSFSTFISLFTAMLLSPLIAWYTKGKYYLARQRPKYLQPNA
                     TKQCVICEREYEVSDMAHCPAYGGAICSLCCCLDARCHDTCKPHGRLSAQWEALNKRI
                     LPKQLWPHLSGGVAHFLLLMVIAVLLFGATISLIYLHDALTIGRESPELMHHFRLGYL
                     KVFVAMLFLGGIVAWWLVLTSASRRVAQEESNRQTGLLLEEIESHKKTDAQLQKARRL
                     AEEANQAKSRYITGISHELRTPLNSILGYAQLLTNDDSIPEHRRQAIRTISRGGEHLL
                     SLIESTLDIARIESGKMAFDVKPLHFPEFISQIVSMFEIQAQNKGLAFYFERQGNLPE
                     YVRADHKRLMQILVNILGNAVKFTTKGSVTFRVSYAREIAQIDIEDTGPGIAPNEVEK
                     IFEPFERGSAADVVGIGGTGLGLTISKLLTQLMGGEMHFNSVMGRGTRFEIRLFLPQI
                     RETAHLSKTAVRQRIGYEGPIRTILVVDNEEVDRELLRNTLQPLGFIVHEAGNGQACV
                     NQYQMIHADLILMDLAMPVMDGWEAAYIIRQVHHAKLPIAIISANAYDRSLDNQAKIP
                     AHDFFVKPVNLEEVLDWIGNQLGLTWRYADSKENKRLSPVIAAASAADGVSLPPIPDV
                     LLENLAQFARMGYVKGVREVLATLNREHSQHQTLINALQQAMERFDFAKLQSLLEEHA
                     K"
     gene            complement(4768..5994)
                     /locus_tag="VNZ84_05760"
     CDS             complement(4768..5994)
                     /locus_tag="VNZ84_05760"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat-containing protein kinase
                     family protein"
                     /protein_id="HWU66497.1"
                     /translation="MTPTPAPDDTLVQLKAGKLQGAKQLKLSAQLTQFPAEIFELADS
                     LEILDLSGNALTDLPSDLSRLKKLRILFCSNNQFTHLPEVLGQCENLGMIGFKANQIK
                     HIAENAIPTQTLRWFIVTDNALSKVPHALGECSKLQKLMLAGNQLSSLPASLANCHAL
                     ELLRISANQFETLPEFLFDLPKLTWLAYAGNPFCNAIEQALISQHPMQHIDWQTLTLQ
                     QVLGEGASGVTYQALMQDKNMQEFVAVKLFKSGLTSDGLPRCEMHANMLVGEHPNLVG
                     VKGVIHNHPQGVDGLVMPLLDADLKVLAKPPSYESCTRDVYANDLTLTPQQAKFILKG
                     ITQAAAHLHAHGLMHGDLYAHNILWGPEKVVLSDLGGASFLPLDQPELTQKILKLEER
                     ALAVLAEELEAVVSGK"
     gene            complement(6175..6738)
                     /locus_tag="VNZ84_05765"
     CDS             complement(6175..6738)
                     /locus_tag="VNZ84_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HWU66498.1"
                     /translation="MKKSILLFLGALAASTSAVAHPGHGLESGFAAGFMHPFSGWDHL
                     LVMFALGIWAARRPAAQGWQLPVLFVTVMALSASLAMAWVPVSLAEILVAASILVIGL
                     LLVSNVKVNRAVQIGVVSIAAAAHGYLHGMEIGNQWSGLVGMVLATAVLHGLGWVLGR
                     QTHPQLQRATQLLGGVMLGLGAVWMLA"
     gene            complement(6916..7569)
                     /gene="ureG"
                     /locus_tag="VNZ84_05770"
     CDS             complement(6916..7569)
                     /gene="ureG"
                     /locus_tag="VNZ84_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HWU66499.1"
                     /translation="MKIHTQFDTPTPAVTEYKEPLRVGIGGPVGSGKTALTLALCQRL
                     REVYNIAVVTNDIYTKEDAEFLTRNEALVPERIIGVETGGCPHTAIREDASMNLEAVD
                     QLSKRFKTLDIVFVESGGDNLAATFSPELSDLTIYVIDVSAGEKIPRKGGPGITKSDL
                     LVINKIDLAPMVGASLEVMDQDAKRMRGEKPFIFSNLKSGQGLEDIVAFIEKQGLML"
     gene            complement(7566..>7928)
                     /locus_tag="VNZ84_05775"
     CDS             complement(7566..>7928)
                     /locus_tag="VNZ84_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HWU66500.1"
                     /translation="RLLNDLKPMSDTLVAQINTLESPAFPTLYAAIAHHWQIPPEQAL
                     HGYAWGWLENQASAAMKAVPLGQVAGQTILLAMGERLPHVVASAMHLPEEHMSNFNPL
                     LSIAGCQHETQYSRLFRS"
BASE COUNT         1993 a         2163 c         1927 g         1845 t
ORIGIN      
        1 cgccggtttg cttcgccacc gcagcaacca cttgggcaac cgcagactca gagcggccat
       61 tgataatcac ctgcgcgcct tcctgcgcca gctgactggc aatggccaag ccaatgccag
      121 cggtggatcc agagacaagt gctgttttgc ctaccagttg caagtccata ttcactcctg
      181 ttttgagtct tcggtgatta aagaatcgta tccgcctaaa acttgatttt tgaagcaacc
      241 agtgaggcgg ctgccgtcct ggtttctacc ccgagcttct ggaaaacatg ctcaaggtgc
      301 ttattgatgg tacgagggct ggtgcccaaa atttcgccca catccttgtt ggttttacct
      361 ttgacagtcc agtacaacac ctcagactca cgcttggtca gctggaacag tttacccagc
      421 gactcaatct gtgcttcatc tgagtattct cgcaacacca ccacccactg ctcttcgctg
      481 gtattggcca cggtcaggct caagcgtgca ttgccctggt tgacgttgag ggtttgcatg
      541 gactgtccac tggaaaattg ctggatacag ctttccagcc acaggtaaat catgggtggc
      601 aaagtttgta ccgcactata ggcttctttg gctgcccatt cagcaaaata ttgctccagg
      661 tattgcttgg ccagcggggt ttgccacacc aggcgtttgt ctgtcggcag gatcgcaatc
      721 gttgcctggc caaaggcatc cagtgctgaa gttgcctggt gcatttttgt cgcattctgg
      781 acatgcacct tgaggcgggc cagcacttct gccggacgta ccggtttggt cacgtagtcc
      841 acaccacccg catcaaacgc catggtcacg tgttctacct cagtcaggcc ggtcatgaaa
      901 atcaccggga tatgttgcgt ttctgccgca gccttgatgc gcctgcaggt ggcaaaacca
      961 tcaaggcccg gcatcatggc atccattaaa attacatgtg gctgcatgag gtgtgcaacc
     1021 tgcaatgcca tctcgcccga gttcgccaca tagacctgat agccggactc atccagtgca
     1081 tcatgcagat aggccaggtt ttctggcaca tcatccacaa ttaacacggt atggtttgga
     1141 ctggtagtca tttggcgtgc tcctcaagca agctttgcag tttagcaaaa tcaaaacgtt
     1201 ccatggcctg ttgtagtgca ttaatcaatg tttgatgctg cgaatgttca cgattgaggg
     1261 tggccaacac ctcacggacg cccttgacgt agcccatgcg tgcaaattgt gccaggtttt
     1321 ccagcaagac atcaggaatc ggtggtaatg atacgccgtc tgctgcagat gcagctgcaa
     1381 tgaccggtga caaccgtttg ttctctttac tatcagcata gcgccaagtc aggcctaatt
     1441 gattgcctat ccaatccagc acctcttcga gattgacggg tttgacaaag aaatcatggg
     1501 ccggaatttt ggcctgatta tccaggctac ggtcataggc attggccgaa ataatcgcaa
     1561 tcggcaactt ggcgtgatgc acctggcgga taatataggc cgcttcccag ccatccatga
     1621 cgggcatggc cagatccatg aggatcaggt cggcatgaat catctggtac tggttgacgc
     1681 acgcttgccc gttacccgct tcatgcacaa taaaacccaa tggctgcagg gtattgcgca
     1741 acaactcgcg atcaacctct tcattatcca cgactaggat agtgcgtatt ggcccctcgt
     1801 aaccgatccg ctggcgtacg gcagtttttg ataaatgtgc agtctcacga atctgcggca
     1861 agaacaagcg gatttcaaag cgcgtgccac gacccatcac gctattaaaa tgcatttccc
     1921 cgcccatgag ctgggtcagc aatttactga ttgtcagccc aaggccggta ccgccaatgc
     1981 caaccacatc tgcagcactg cctcgctcaa acggttcaaa aattttctca acctcgttcg
     2041 gcgcaatccc tgggccggta tcttcaatat caatctgtgc gatttcgcgt gcataactga
     2101 cgcggaaagt aacactgccc ttggtggtga acttgaccgc attgcccagg atattgacca
     2161 gaatctgcat caagcgttta tggtctgcac gaacatattc cggcaaattg ccttgccgtt
     2221 caaaataaaa tgccaatcct ttgttttgtg cctggatttc aaacatgctg acaatctggc
     2281 taataaactc gggaaagtgc agcggcttga catcaaacgc catcttgccg ctctcaatac
     2341 gtgcaatatc cagcgtactc tcaatcagtg ataacaaatg ttcgccacca cggctaatgg
     2401 ttctaatcgc ctgcctgcgg tgctcgggaa tactgtcatc gttagtcagc aattgtgcat
     2461 aaccaaggat actattcaag ggagtacgca actcatgact aatgccagta atatagcggc
     2521 tcttggcctg gttggcctct tccgccaggc ggcgcgcctt ttgcagttgc gcatcggttt
     2581 tcttgtgcga ctcgatttct tcaagcagca aacccgtttg ccggttggac tcttcttgtg
     2641 ccacacgccg gctggctgag gtcaacacca gccaccaggc gacaatcccg cccaaaaaca
     2701 gcatggccac aaataccttt agatagccga gcctgaaatg atgcatcaac tctggagact
     2761 ctcgaccaat cgtcaaggca tcatgcaggt aaatcaggct gatcgtggcc ccaaataaaa
     2821 gtaccgcaat caccatcaac aacaaaaagt gcgccacccc gccactcagg tgcggccata
     2881 gctgtttggg taaaatcctt ttgttcaacg cctcccattg cgcactcagg cggccatgcg
     2941 gcttgcaggt atcatggcag cgggcatcca ggcagcagca aagcgaacaa atcgcaccac
     3001 catatgccgg gcagtgcgcc atatcactga cctcatattc acgctcgcaa atgacacact
     3061 gcttggtcgc attgggttgc aaatatttgg ggcgctgcct ggccaggtaa tatttacctt
     3121 tggtgtacca tgcaattaaa ggcgacagca gcatggcggt gaatagggag ataaaagtcg
     3181 aaaaagactg ggccagcgga cccatggcac cggtataagc cacaatagac aacgccgaag
     3241 ccataaacat cgcccccaca cccacgggat tgatgtcata taaataggca cgcctgaact
     3301 ctatcccttt aggactcagg cccaaaggct tgttgatcac caagtcagcg acgactgccg
     3361 caatccaggc aatggcaatg ttggcatata gccccaagat ttcttccagc gcctgaaata
     3421 cattgagctc catgagcaca atggcgatca tcacgttaaa cgccacccag acaatccggc
     3481 caggatggct atgcgtcagg cgggcaaaaa agttagacca cgccagcgag ccggcatacg
     3541 cattagtcat attcaccttg atttgcgata cgcagacaaa gagcgccgtc actgctaggg
     3601 ccacttcagg gttactgaac acatgcccga atcccaccca atacatatgc gtcgggttga
     3661 tcgccgaaga cagcggcata tcgcgattaa acaccatata ggccagcagc gccccggcaa
     3721 acattttgag catgcccaga atcacccatc ctggcccggc catcaatacc cccagattcc
     3781 agcgccagta attttcctta ttgcgctccg gcataaaacg caggaaatcg acctgctcac
     3841 caatttgcgg aatcagggcc acacccactg ccgtcgccgc gccaaacaac atccagttga
     3901 actccccacc attggcagaa ataccggcaa aatgactcag tctgtcgaag attccgggat
     3961 ccatcttgag aatcgccagg aaaggcaaaa acatcaaggc aatccagatc ggttgcgtaa
     4021 tcatttgtat gcggctaatc agcgtcaccc catgcgtgac cagcggaatc acgatcagcg
     4081 cactgaccag ataacccaaa taaagcggca ggtggaaata tagctccagc gcatacccca
     4141 tgatcgcggc ttcaagcgaa agcaaaataa aggtaaacga ggcatatacg aacgaggaaa
     4201 tggttgagcc tatgtagcca aaaccagccc ctcgcgtcag caggtccata tccaggccat
     4261 acttggccgc gtaataactg ataggcaagc cggttaggaa aatcatcagg ccaacagcca
     4321 gaatcgccca catggcattc ggaaaaccat agttgatggt gatcgtgcca ccaatcgctt
     4381 ccagcaccag aaacgaaatg gcccccagcg ccgtattgga aacccgaaga atagaccatt
     4441 tacggaatgc ccgcggcgtg tagcgcaagg catagtcttc tatggtttca ttggccaccc
     4501 agttgttata gtcgcgacga atcttgacga tacgctgtat cggctcagca cgtatttcgg
     4561 tagataatcc agaagtggac ggggtagaca atcaacaatc cttaagtatc aatagccagg
     4621 ttgggaacaa ggccagcttt ttcccgagga tatactagga gagttcactc atgcaatcac
     4681 tacgttaatt gacgtaggag atgtgccacg aaggacgagc gccagaataa tcaaaatccg
     4741 gcacaaagcg atctctgcat acacccatca cttcccactc acaaccgctt ccaactcctc
     4801 agccaaaacc gctaacgccc tttcctccag tttaagtatt ttttgcgtga gctccggctg
     4861 gtccagcggt aaaaaagagg caccgcccaa atcacttaac acgactttct caggccccca
     4921 caaaatatta tgcgcgtata aatcgccgtg catgaggcca tgggcgtgca ggtgtgctgc
     4981 ggcttgcgtg atccctttta aaataaattt ggcttgttgc ggcgtgagcg ttaagtcatt
     5041 cgcgtataca tcgcgcgtgc agctctcata gctagggggc tttgccaaca cttttaaatc
     5101 ggcgtctagg aggggcatga ccaagccatc aacgccttgc gggtgattgt gtattacgcc
     5161 tttaacgccg accaaattag ggtgttcgcc cacaagcata ttggcgtgca tttcgcaccg
     5221 aggcaggcca tcgctggtga gtccactttt gaagagtttt actgccacaa actcttgcat
     5281 gtttttgtct tgcattaacg cctggtaggt gacaccagag gcgccttcac ctaagacttg
     5341 ctgcagcgtg agggtttgcc agtcgatgtg ctgcatggga tgttggctga ttaacgcttg
     5401 ctcaatagcg ttacaaaacg ggttgccagc ataggcgagc caggtgagct tgggtaagtc
     5461 gaataaaaat tcgggcaagg tctcaaactg gttggcagaa atccgcaata actctaaagc
     5521 gtggcaattg gccaggctgg cgggcaagct gctgagctgg ttgcccgcca gcatgagttt
     5581 ttgcagcttg ctgcactcac ccaaggcatg cggcacttta ctcagtgcat tatcagtgac
     5641 gataaaccag cgtaaagttt gggtggggat ggcgttttca gcgatgtgtt tgatctggtt
     5701 ggctttaaaa ccaatcatgc ctaagttttc acattgcccc agcacttctg gtaggtgcgt
     5761 aaactggttg tttgagcaaa aaagaatgcg cagttttttc aggcggctta aatcactagg
     5821 caagtcagtg agcgcattgc cggacaagtc caggatttcc aggctatcgg ctaactcaaa
     5881 aatctcagcg gggaactggg ttaactgcgc acttaatttg agctgtttag ccccttgaag
     5941 tttgcctgcc ttgagttgaa cgagggtatc gtctggcgct ggggtgggtg tcatcacaat
     6001 gcatgtattt tatggatgcg ctatgttact tcaattactt tggatgcaag gcgaactgag
     6061 tgtctagaag caaaaataaa agccagcagt gacactgact tttattgtgc agaggcaatt
     6121 aggaactacg ccgaatacgc gcagcacctt atcggtgtat accctattac gacgctacgc
     6181 cagcatccac acagcgccca agcccagcat cacgccgccc agtaattgcg tggctctttg
     6241 caattgtgga tgcgtctgac ggcccaatac ccaacctaag ccatgcagca cagccgttgc
     6301 cagcaccatg cccaccaggc ctgaccattg gttgccaatt tccatgccat gcaaatagcc
     6361 atgtgcagcg gcagcgatgc tgacaacgcc gatttgcacg gcgcggttca cttttacatt
     6421 gctcaccagt aacaagccta tgaccaagat gctggcagcg accaggattt cagccaagga
     6481 aaccggtacc cacgccatgg cgagtgaagc tgacaaggcc atgacagtga caaacaacac
     6541 gggcaactgc cagccttgcg ccgccggacg gcgtgctgcc cagatcccca gcgcgaacat
     6601 caccagcaag tggtcccaac cactgaatgg atgcatgaag ccagcggcaa agccagattc
     6661 cagtccgtgg ccggggtgtg caaccgcact ggttgaagct gcgagcgcac ctaaaaataa
     6721 aagaatactt tttttcattg aatcttctcc aaacgaattg cattgcgatg agtcagtctt
     6781 gcacccacca ttttttcact tcgatttcac cccgaaaatt ggcaatacgc ggttatggca
     6841 aggcgtatcg gcgcagacag tacaactagt acggcaagcc gatacaacac cgccacagac
     6901 gcgtagtgcc aattttcaaa gcatgaggcc ttgtttttcg atgaatgcga cgatgtcttc
     6961 caacccctgc ccactcttca gattgctgaa aataaaaggt ttctcgccgc gcatgcgctt
     7021 ggcatcctga tccatcacct ctagcgaagc gcctaccata ggcgccaagt ctattttgtt
     7081 gatcaccagc aagtccgact tggtgatgcc aggaccgcct ttacgtggga ttttctcgcc
     7141 agctgatacg tcaatcacgt aaatggtcag gtcagataat tctgggctaa acgtggctgc
     7201 caggttatca ccgccacttt ccacaaacac gatatccagc gtcttaaagc gcttgcttaa
     7261 ttgatccacc gcttccaggt tcatggaagc atcttcacgg atggccgtat gcgggcaacc
     7321 accggtttcc acaccaataa tgcgttctgg caccagtgct tcgttgcgag tcaggaactc
     7381 ggcatcttcc ttggtataaa tatcgttagt caccacggcg atgttatata cctcgcgcaa
     7441 gcgctggcac aacgccaagg ttaaagcagt tttgccggaa cccaccgggc cgccgatacc
     7501 aacgcgtaga ggctccttgt attcggtcac ggccggggtg ggtgtatcaa attgggtatg
     7561 tattttcatg atcggaataa cctgctgtat tgtgtttcgt gctgacatcc ggcgatgctc
     7621 agcagtggat tgaagttgct catatgctcc tctggcaaat gcatggcact cgctaccacg
     7681 tgtggaagcc gttcacccat tgccagtaaa atggtttgcc ctgcgacctg gcctaatggt
     7741 acagccttca tggcggcact ggcctggttt tccaaccagc cccaggcata gccatgcaat
     7801 gcttgctcag ggggaatctg ccaatgatgt gcgatggcgg catacaaggt tggaaaagcc
     7861 ggtgactcca atgtattaat ctgcgcgacc aatgtgtctg acatgggttt taaatcattc
     7921 agtaaccg
//
LOCUS       DATMSK010000173         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_200671,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000173 DATMSK010000000
VERSION     DATMSK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200671"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VNZ84_05780"
     CDS             complement(<1..755)
                     /locus_tag="VNZ84_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWU66501.1"
                     /translation="MKRLFWILLLINVAVFAYFKRDALSPTVSSLKPELHPEQIKLVD
                     AKTLESYPKRLSEPAPAPEPTVTPEEPSPAQTALAPSLPKPESKQADSKLPEKTPAKP
                     AETHASAPTACYEWSGFTMSRVTEATNAAQQLNIKTQANMTSSSQENVRYWIYKPPLA
                     TPEAAQAKAEELRKLGVEDFFIVQDDPKWRNAISFGVFRDEKLADKLMADLKNKGVKL
                     LIKAPRNGGQAVIKLQQVSPQQFASLQKSRAHFP"
     gene            complement(772..1542)
                     /locus_tag="VNZ84_05785"
     CDS             complement(772..1542)
                     /locus_tag="VNZ84_05785"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWU66502.1"
                     /translation="MKLLIDAGNSRTKWAWCPASFGDEASWQFHAVENAEWLAASGKA
                     ESLRQAVIEADQVWLSNVAGERWLQALPIIMKSLHVVQATGAALGLRNHYSQPAQLGS
                     DRWCSLLAVWRQFGQSALVVTAGTAMTIDALLVSEHEANFLGGSIQPGLRMMWQSLQQ
                     GAAQLDYAYPEDIQWEAFARNSQQAMWLGCVQALTAAIAMQYARLSDQARTSPLLLLS
                     GGDAQLLCRYLPHALSAQAIIVDNLVLKGLACLAENTD"
     gene            complement(1539..2528)
                     /locus_tag="VNZ84_05790"
     CDS             complement(1539..2528)
                     /locus_tag="VNZ84_05790"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWU66503.1"
                     /translation="MNKLAFPILAVLADGKFHSGEALAQRFGVTRATVYNAIQSVEVL
                     GITVFSVRGRGYRLPKPVTLLDVEEIAHACGEYAPWFHIQVLAQVDSTNRYLMQESAH
                     GVAHGTCVAAQIQTAGRGRRGRSWLAQLGGSLTFSLLWRFQCGAAGLSGLSLVVGLAL
                     VRALHGLGLTDVQLKWPNDLLVKHQGQWHKLGGILIELQGDMEGPSAAVIGIGLNLEM
                     DADIRQQIEQPVMGLNTLFGQSLDPNLLLGRCLSQLAIHLNQFAEHGFAYFQAEWTSY
                     HAFHQQAVNLLLASGQVVPGQVLGVAEEGSLLVETAAGQQRFNAGEISVRSAA"
     gene            2709..>2959
                     /locus_tag="VNZ84_05795"
     CDS             2709..>2959
                     /locus_tag="VNZ84_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HWU66504.1"
                     /translation="MSTEKEPIPAASNFIRAIIDKDLAEHKYATKKWAGSPGDAAHQA
                     TGQVDFAKIRTRFPPEPNGYLHIGHAKSIFLNFGLARDY"
BASE COUNT          678 a          868 c          702 g          711 t
ORIGIN      
        1 gggaagtgcg cgcgggactt ctgcaggctc gcaaactgtt gtggtgatac ctgttgcagc
       61 ttgatcaccg cctgcccacc attccgggga gctttaatca gcagtttcac ccctttattt
      121 ttgaggtctg ccatcagttt atccgccagt ttctcatcac ggaagacgcc aaacgaaatc
      181 gcattccgcc atttagggtc atcctgcaca atgaagaaat cctccacgcc gagtttgcgt
      241 aactcttcag ccttggcttg ggccgcttcg ggtgtcgcca atggtggctt gtatatccag
      301 tagcgtacgt tttcctggct gctgctggtc atattggctt gcgttttgat attcagctgc
      361 tgtgccgcgt tggtggcttc agtgacgcgt gacatagtaa atccgctcca ctcataacaa
      421 gcagttggtg ctgaagcatg cgtttcagct ggtttggcgg gtgttttttc cggtaattta
      481 gagtctgcct gtttgctttc cggcttgggc aagctgggtg ccaaggcagt ttgcgcaggc
      541 gatggctctt ccggcgttac cgttggctca ggtgctggcg caggctcgct gaggcgcttt
      601 gggtaagact ctaacgtctt ggcatccacc agttttattt gttcgggatg cagttccggc
      661 ttcaggctac tgactgtcgg cgataacgca tcgcgtttga agtaggcaaa tacagccaca
      721 ttgatcaata gcaatatcca gaatagtcgt ttcattgaac tgtacttttc attaatctgt
      781 attttcggcc aggcaggcca acccctttaa taccagatta tccacaataa tcgcctgtgc
      841 tgacaatgca tgtggcaaat atctgcacaa caactgcgca tccccaccgc tgagtagcaa
      901 taatggtgat gtccgggcct ggtctgacaa tcgtgcatat tgcatggcaa tcgccgctgt
      961 cagtgcttgc acgcagccta accacatcgc ttgctgacta ttacgcgcaa atgcctccca
     1021 ctgtatatct tctggatacg cataatcgag ttgcgcagct ccctgctgca aactttgcca
     1081 catcatcctc aagcccggct gtatgctgcc acccagaaaa tttgcttcat gttcgctgac
     1141 cagcaaggcg tctatcgtca tggcggtgcc tgcagtgacc accagcgcgg actggccaaa
     1201 ttgccgccac acagccagca ggctacacca gcggtcgctg cctaattgtg ccggttggct
     1261 gtaatgattt cgtaaaccca gggcggcacc ggtcgcctgc accacatgca aagacttcat
     1321 tatgattggc aatgcctgca accagcgttc gccggcgaca ttgctaagcc agacctgatc
     1381 ggcctctatg acggcctgtc gtaacgactc agctttgccg cttgctgcaa gccactcggc
     1441 attttccact gcatgaaact gccatgatgc ctcgtcaccg aatgaggctg gacaccatgc
     1501 ccatttggtc cggctattgc ccgcgtctat caataatttc acgctgcgct ccggacactg
     1561 atttcaccgg cattaaaccg ctgctgacca gcggccgttt ctaccagcag actgccctct
     1621 tctgccacgc ctaacacttg cccaggcacg acctgcccac ttgccagcaa cagattgact
     1681 gcctgttgat gaaacgcatg ataacttgtc cattcggctt gaaaataggc aaacccgtgt
     1741 tctgcaaact ggtttaaatg aatcgcaagc tgactgaggc aacggcccaa taacaagtta
     1801 gggtccaacg attgaccaaa cagtgtattc aggcccatga ctggctgttc aatctgctgg
     1861 cgtatatcag catccatttc caggttcagg ccgatgccaa tcactgccgc actcgggcct
     1921 tccatatcgc cttgcaactc aatcaaaatg ccacccagtt tatgccactg cccctggtgt
     1981 tttaccagca ggtcattcgg ccatttgagt tgcacatccg tcaatcccaa accatgcagg
     2041 gcacgcacca acgccaggcc cactacgagg cttagacctg acaagcctgc cgccccacat
     2101 tgaaaccgcc acaatagtga aaacgtcagg ctaccaccca gctgcgccaa ccagctacgg
     2161 ccacgacggc cacgccccgc agtctggatt tgtgcagcga cacacgtacc atgtgctaca
     2221 ccgtgtgcgc tctcctgcat caggtaacga ttggtggagt ctacctgtgc cagcacctga
     2281 atatgaaacc atggtgcata ttcaccgcag gcatgcgcaa tctcctccac atccagcaag
     2341 gtcacaggct tgggtaagcg gtaaccgcgt ccgcgtactg aaaacaccgt gatccccagc
     2401 acttcaaccg attgaatggc gttatacacc gtggcacgcg tcacgccaaa gcgctgtgcc
     2461 agcgcctcac cggagtgaaa tttgccatcg gccaacacag ccaatatcgg gaatgccagt
     2521 ttgttcatca aatgttgttg agatcgtttt attgaggtga gccagtggcc atcatcttag
     2581 cccgcagtgt agcatacccg ccttgcagac ttggccgata aaagcccgaa atcccatgaa
     2641 ttaagcgttt tgggcccagt cacgaagcgt gtaaaataga ccttacttta gaaacttcaa
     2701 gaatcgccat gtctacagaa aaagaaccta ttcccgctgc ctccaacttt atccgcgcca
     2761 tcattgataa agacctcgca gaacacaagt acgcgaccaa aaaatgggca ggcagcccag
     2821 gcgatgccgc gcatcaagcg accggccagg tagattttgc caaaatccgc acccgttttc
     2881 cgcccgagcc taacggctac ctgcatattg gtcacgccaa atctatcttc ttgaattttg
     2941 gcctggcaag ggattacga
//
LOCUS       DATMSK010000174         4807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_36479,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000174 DATMSK010000000
VERSION     DATMSK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36479"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2999)
                     /locus_tag="VNZ84_05800"
     CDS             complement(<1..2999)
                     /locus_tag="VNZ84_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HWU66505.1"
                     /translation="MNAPVNADLLKHDSTPLTRLREIAYNYTSFSDREIVIRFLGEEV
                     WGILNVLRAERKTGRSARMLFEVLGDLWVVSRNPYLQDDLLENPKRRKALIEALYHRI
                     SAINERSGGNDKVQKLVSAATKAVEKFADDFKQTAELRKKALAKLTKFTRKDNVQFDG
                     LARVSHVTDATDWRVEYPFVVINPDTEAEISYLVRACIELGLTIIPRGGGTGYTGGAV
                     PLTPLSAVINTEKLDQHTGVQMRTLPGVSRQVATIECGAGVVTRRAMDAATAAGLEFA
                     CDPTSADACCIGGNVAMNAGGKKAVLWGTALDNLASWRMVTPDATWLEVERLDHNLGK
                     IHDIETARFKISRYDIDFKNLLSEPEILEIPGPSFRKVGLGKDVTDKFLSGLPGVQKE
                     GCDGLITSATFILHRMPKHVRTIALEFFGNVSHAVPAIVEIKDYLDATAKNDPAVIMA
                     GLEHMDERYVKAVGYATKAARQQRPKMVLLCDIASDDENAVGEVASAVVRMCNARNGE
                     GFIAVSAEARKKFWLDRSRTAAIARHTNAFKINEDVVIPLPRLGEYSDGIERINIELS
                     INNKLRLVDELEAFVSGDLPLQPDEDGNADPEGVKSKQNIALQLLRDVRARWRLLLNT
                     LDEPVSVLGEMGKPFAQYENVFRAVQSYDLRVSWKRELKRPMADIFAGREYQKILDKL
                     DEIQKAVLKSRVFIALHMHAGDGNVHTNIPVNSDDYGMMQDAHAAVARVMDLARGLNG
                     VISGEHGIGITKMEFLDQATIDTFAKYKAKVDPEGRFNKGKLMAGSGLENAYTPSFGL
                     LEQESIIMEQSAIGEIADDISDCLRCGKCKPVCSTHVPRANLLYSPRNKILGTSLLIE
                     AFLYEEQTRRGISLKHFDEFNDVADHCTVCHRCEKPCPVNIDFGDVSVKMRNFLREQG
                     KKKFNPGTAAGMMFLNVKDPATIKLLRGTMIQMGYKAINIANSLAKKFGLLKDKKAPP
                     ATVGRAEIKAQVVHFINRPMPKKMQSKT"
     gene            3095..3601
                     /locus_tag="VNZ84_05805"
     CDS             3095..3601
                     /locus_tag="VNZ84_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWU66506.1"
                     /translation="MSTLKSKFKKWVVPLVALILFAGLAVAVMQKPQAPEVTFTTLTG
                     EKISMQALRGKTVLVNFWATDCPGCIKEMPELIDTYQQYKDKNFVVIAVAMPYDPPAQ
                     VANYTKEKNLPFAVMHDGYGEMVKAFGDVNLTPTSFIFDAQGNRLQKTIGELNFAELR
                     KLLNQKAR"
     gene            3602..4012
                     /locus_tag="VNZ84_05810"
     CDS             3602..4012
                     /locus_tag="VNZ84_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWU66507.1"
                     /translation="MLWVKAFHIIFVTSWFAGLFYLPRIYVNLAMEQDTQAHARLILM
                     ANKLYRFMQPLMLLALAFGIWLWLGYGFKGVWLHAKLTLVVLLIGYHHYCGRLLKQFR
                     AQQNRHSHVWFRWFNEVPVLILFAVVILVVTKPF"
     gene            4017..4772
                     /locus_tag="VNZ84_05815"
     CDS             4017..4772
                     /locus_tag="VNZ84_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66508.1"
                     /translation="MLNALLQSEWLIRMLDKSGKQASHKLLGLFNILDDLLSSPLSGA
                     NVQQMVQPATDAASSDRLQNYLTTQAQLAGARLPDMLANQLYFMALSACQEKLLHKQS
                     SALIHARQAAQALIDEQTKHEINWLAIRNYSIVAVALFSGVIGGYYWHMQYATSPVAQ
                     AAIEEPPPTTPMSHEASPTETAAIYTRLETMKHGDCRLLEALQLPDRVKSVYIENIIN
                     GHVTTNRDEQVLVNQLLDQVQCNYTPKLMANSK"
BASE COUNT         1227 a         1296 c         1145 g         1139 t
ORIGIN      
        1 gttttggatt gcattttctt cggcatcgga cggttgatga aatgaaccac ctgcgccttg
       61 atttctgccc gacccaccgt cgctggcggt gcctttttat ctttgagcaa gccgaacttc
      121 ttagccagac tattggcgat gttaatggct ttatagccca tttgtatcat ggtgccgcgc
      181 agcaatttga tcgtcgccgg gtctttgaca ttcaagaaca tcatgcccgc tgcagtgcca
      241 ggattgaatt tctttttacc ctgttcgcgc aggaaattgc gcattttcac ggacacatcg
      301 ccgaagtcga tattgaccgg gcacggtttc tcgcaacggt ggcagaccgt acaatggtca
      361 gccacgtcgt tgaattcgtc aaaatgcttg agcgaaatac cgcggcgcgt ctgctcttca
      421 tacaagaagg cttcgatcag caacgaagtc cccagaatct tgttacgtgg ggaatacagc
      481 aggttagcgc gtggcacgtg ggtagaacac acaggcttac acttgccgca acgcaagcag
      541 tcagaaatat catcggcgat ctcgccaatg gcagactgtt ccataatgat ggactcttgc
      601 tccagcagac caaagctagg cgtgtaggcg ttttccaggc ctgagcccgc catcagtttg
      661 cctttgttaa agcggccttc agggtctacc ttggctttgt atttggcaaa ggtatcaatg
      721 gtcgcctgat ccaggaactc catcttggta ataccaatac cgtgttcacc agaaatcaca
      781 ccattgaggc cacgcgccag gtccatcacc cgtgcgaccg ctgcgtgtgc atcctgcatc
      841 atgccgtagt catcggagtt caccggaata ttggtatgca cgttaccatc accagcgtgc
      901 atgtgcagcg caataaagac gcggctcttc aacacggctt tctggatttc gtccagcttg
      961 tcgaggatct tctggtattc gcggccagca aaaatatccg ccatcgggcg cttgagttcg
     1021 cgcttccagg agacgcgcaa atcataagac tgcacggcac ggaacacgtt ttcatactgt
     1081 gcaaatggtt tacccatttc gcccagtaca gacaccggct catccagcgt attgagcaac
     1141 aagcgccatc tggcgcgtac gtcgcgcaac aattgcaagg caatattttg cttggatttc
     1201 acaccttctg ggtcagcgtt gccgtcttcg tccggctgca aaggcaaatc gccagacaca
     1261 aaggcttcca actcatcaac taaacgcagt ttgttgttga tggaaagctc aatattgatg
     1321 cgctcaatgc catcagagta ttcgcccagg cggggtaatg gaatcaccac gtcttcgtta
     1381 attttgaaag cattggtgtg acgagcaatc gcagcggtac gcgagcgatc caaccagaat
     1441 tttttacgtg cttccgctga aacggcaata aaaccttcac cgttacgtgc gttacacata
     1501 cgcaccacag cagacgccac ttcaccgaca gcattttcat cgtcagaggc gatatcgcac
     1561 aacaacacca ttttcggacg ttgctgccgt gcggccttgg tcgcatagcc aaccgctttc
     1621 acatagcgct cgtccatgtg ctccagaccc gccataatca cagcaggatc gtttttagcc
     1681 gtagcgtcca gatagtcttt aatctcaaca atggccggca ccgcgtgtga gacgttacca
     1741 aagaactcca gcgcaatggt acgcacatgc ttgggcatgc ggtgcaaaat aaaggtggct
     1801 gaagtaatca ggccatcaca accttctttt tgcacgccag gcaagccaga cagaaacttg
     1861 tcggtcacat ctttacccag gcccacttta cggaagcttg gaccagggat ttccaggatt
     1921 tcaggctctg acaacaggtt tttgaaatca atgtcgtaac ggctaatctt gaagcgcgcc
     1981 gtctcgatat cgtgaatctt gcccaggttg tgatccaggc gctccacttc cagccaagtc
     2041 gcgtccgggg taaccatgcg ccaggaagcg aggttatcca gcgcagtgcc ccacagcacg
     2101 gcctttttac cgccagcatt catggcgacg ttaccgccaa tgcagcacgc atcggcagaa
     2161 gttgggtcac atgcaaactc cagcccagcc gcagttgcgg catccatggc acggcgggtg
     2221 acaacacccg caccacactc aatcgtggct acctgacgtg acacgcctgg caaggtgcgc
     2281 atttgcacgc cagtgtgctg atccagtttt tcagtgttga tgacggcaga taatggcgtc
     2341 aatggtaccg cgccaccggt ataacccgta ccaccgcctc gcgggatgat ggttaagccg
     2401 agttcgatac aggcacgcac caggtatgaa atttcagcct cggtatccgg attgatcacc
     2461 acaaacggat actcaacgcg ccagtcggtc gcatcagtca catgtgagac gcgcgccagg
     2521 ccatcaaact gcacattatc cttgcgcgta aacttggtca gtttagctaa ggctttttta
     2581 cgtaattcag ccgtttgctt gaagtcatcg gcaaactttt caacagcttt ggtcgcggca
     2641 gagaccagtt tttgtacttt atcgttacca ccgctgcgtt cattaatggc agaaatacgg
     2701 tggtacaaag cctcgatcaa agctttgcga cgcttaggat tttccagcaa atcatcctgc
     2761 aggtaaggat tgcgagacac tacccacaga tctcccagca cctcaaacag catgcgtgcg
     2821 ctacggccgg ttttgcgctc agcgcgcaag acattcaaaa taccccaaac ctcttcgccc
     2881 agaaaccgaa taacaatttc acggtcagag aaggaagtat agttgtacgc gatttcacgt
     2941 aaacgcgtga gaggagtaga atcgtgcttt aacaaatctg cgttgacagg tgcgttcatt
     3001 gataatttat caaaaacaaa agtcaattat aacacgccgc atacggcaca aacacgttaa
     3061 acggtctagg gtttttgagt aaacaggttt tcggatgtcg acattaaaat ctaaatttaa
     3121 aaaatgggta gtgccactgg ttgcgttgat attgtttgcc ggtttggcag tggcggtcat
     3181 gcaaaaacct caagcaccgg aagtcacctt tacgacgtta acgggtgaaa aaatcagcat
     3241 gcaagcgctg cgcggcaaaa ccgtcctggt gaatttctgg gccactgatt gcccaggctg
     3301 catcaaagaa atgccagaac tcattgacac ataccagcag tataaagaca aaaatttcgt
     3361 cgtgattgca gtagccatgc cctatgaccc tccggcgcaa gtagccaact acaccaagga
     3421 aaaaaacctg ccattcgccg tgatgcatga tggctatggc gaaatggtta aggcatttgg
     3481 tgacgtcaac ctcactccaa ccagcttcat ttttgatgcg caaggcaacc gcctgcaaaa
     3541 aaccatcggt gaactcaatt tcgctgaact gcgcaagcta cttaaccaga aagcccgcta
     3601 aatgctgtgg gtgaaggcgt ttcacatcat ctttgtgacg agctggtttg ccggcttgtt
     3661 ttacctgcca cgtatttacg tcaacctggc catggagcaa gatactcaag cccatgcccg
     3721 cctaatcttg atggctaata aactttaccg ctttatgcag ccactgatgc ttctggcatt
     3781 ggcctttggc atttggttat ggctgggata cggtttcaaa ggtgtatggt tacatgccaa
     3841 gctcacatta gtagtgctgc tgattggcta tcaccattat tgcggcaggt tgcttaaaca
     3901 gtttagagcc cagcaaaacc gccacagcca tgtctggttc cgctggttta atgaggtacc
     3961 ggtgctgatt ttatttgccg tcgtcatcct ggtcgtgacc aaaccattct gatcacatgc
     4021 tcaacgcgct gctgcaatct gaatggctga tacgcatgct ggataagtcc ggcaaacaag
     4081 cttcccataa actgctgggc ctgtttaaca ttctggatga tctgttatcc agcccgctat
     4141 ccggtgcaaa tgtgcagcag atggtgcaac cagcaactga tgccgcctca tctgacaggc
     4201 tgcaaaacta cctcacgaca caagcacagc ttgccggcgc cagactgcct gatatgctgg
     4261 cgaatcagtt atatttcatg gcgctcagcg cctgccagga aaaattgctg cacaagcaat
     4321 ccagcgcact tatccacgcg aggcaagcag cgcaggcact gattgacgaa caaacaaagc
     4381 atgagattaa ctggttagca atacgtaatt acagcattgt ggccgtcgca ctattcagtg
     4441 gtgttattgg cggttactac tggcatatgc aatatgccac atctccagtt gcgcaagctg
     4501 caatagaaga acccccaccc acaaccccga tgtcacacga agcgagccca acagagacag
     4561 ctgccatcta tacacgcctg gaaaccatga agcacggtga ttgccgcctg cttgaggcct
     4621 tgcagctacc tgaccgggtt aaaagcgtgt atatcgagaa catcatcaat gggcacgtca
     4681 ccaccaaccg cgacgaacaa gtactcgtga atcagttact ggatcaggtg caatgtaatt
     4741 acacgccaaa attgatggcc aactccaaat aaaaaggccg cctgacgacg gcctcttgca
     4801 tcagaac
//
LOCUS       DATMSK010000175         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_82993,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000175 DATMSK010000000
VERSION     DATMSK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82993"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..678
                     /gene="ahpC"
                     /locus_tag="VNZ84_05820"
     CDS             115..678
                     /gene="ahpC"
                     /locus_tag="VNZ84_05820"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HWU66509.1"
                     /translation="MSLINTQVQPFKAQAFHNGKFIEVTEESLKGKWSVLIFMPAAFT
                     FNCPTEVEDAADNYAEFQKAGAEVYIVTTDTHFSHKVWHETSPAVGKAKFPLVGDPTH
                     QLTRAFDVHIDEEGLALRGTFVINDEGVIKTAEVHSNEIARDVKETLRKLKAAQYTAA
                     HPGQVCPAKWNDGAATLTPSLDLVGKI"
     gene            853..2418
                     /gene="ahpF"
                     /locus_tag="VNZ84_05825"
     CDS             853..2418
                     /gene="ahpF"
                     /locus_tag="VNZ84_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HWU66510.1"
                     /translation="MLDDTLKTQLQTYLGMLRQPIRLIASLDNSDNAAKMRELLQEIT
                     ALSDKVSLDESGDDARKPSFVIAKEGETRGVRFAAIPLGHEFTSLVLALLWTGGHPPK
                     VEQDVLDQIKALDNDLNFEVYMSLSCHNCPDVVQATTLMTVFNPRINVTVIDGGLYQD
                     EVNERNVMAVPATFLNGEMFASGRMLVEEFLAKVDTGAVAKEAEKISAREPFDVLVVG
                     GGPAGASAAIYAARKGIRTGIVADRFGGQVNDTLAIENFISVKETEGPKLVAALEEHV
                     KTYDVDIMPLQRAVGLAEPSKADNKHAGLIEVKLESGATLRSKSVILATGARWKNLNV
                     PGEVEYKNKGVAYCPHCDGPLFKGKHVAVIGGGNSGVEAAIDLAGVVGHVTLLQFDTE
                     LKADAVLQRKLRSLQNVTILTSAQTTEITGDGTRVNGLTYTDRVSGESKHVALEGVFI
                     QIGLVPNSDWLKGTLNLSKYGEIEINNHNATSLPGVFAAGDVTTIPYKQIVIAMGEGS
                     NAALGAFDYLIRQ"
     gene            complement(2473..4104)
                     /locus_tag="VNZ84_05830"
     CDS             complement(2473..4104)
                     /locus_tag="VNZ84_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU66511.1"
                     /translation="MLRITEIKLPIELADTLKHQDDQIKAAILKRLEIPETELIHFEI
                     FKRGVDARKSHAILYVYNIDVEVRNEARLLAKFKKDPHIKPAPDTHYKFVARQETNTH
                     ERPVVVGFGPAGIFAALILAQAGFNPIVLERGKEVRKRTKDTWGLWRKRELNPESNVQ
                     FGEGGAGTFSDGKLYSQIKDTKHYSRKVLHEFVKAGAPEEILYVSHPHIGTFRLVGMV
                     EEMRKTIIALGGEIRFESRVTDIAIEHDQVQGVTLHTGEFIPTRHLVLAVGHSARDTF
                     EMVYEKGIYVEAKPFSIGFRIEHPQSLIDRARYGKSYSDDILQKLGAADYRLVHHAKN
                     GRSVYSFCMCPGGTVVAATSEPNRVVTNGMSQYSRNERNANAGIVVGITPEVDYPDHP
                     LAGVAFQRQLESNAFVLGGSNYSAPGQLIGDFLANRPSTKFGEVMPSYTPGVHLTNLD
                     TALPEFAISAMREAIPAFAKQVPGFDLTDGVLTGVETRTSSPIRIKRDDDTLQSINTK
                     GLFPCGEGAGYAGGILSAAVDGIKVAEAVSLSLVG"
BASE COUNT         1089 a         1027 c         1130 g         1090 t
ORIGIN      
        1 attttacata ttatatatgt gtgtctaaaa tgcactcatt cgctgttgaa acacagcaca
       61 ttttaattaa ccctgtcatt aaccaatgtt ttataaatca taaaggagaa ctacatgtca
      121 ttgattaaca cacaagtaca accattcaag gcgcaagcat tccacaacgg taaatttatc
      181 gaagtgacag aagagtcttt gaaaggcaaa tggtctgtgt tgattttcat gccagcagcg
      241 ttcactttca actgcccaac agaagttgaa gatgcggctg acaactacgc agaattccag
      301 aaggctggtg ctgaagtgta catcgtgacc accgataccc acttctccca caaggtttgg
      361 cacgagactt cacctgcagt aggcaaagcg aaattcccac tggtcggtga cccaacacac
      421 caattgacac gtgcatttga cgtgcatatt gatgaagaag gcctggcatt gcgcggtacg
      481 ttcgtcatca atgacgaagg cgtgatcaag actgctgaag tacacagcaa cgaaatcgcc
      541 cgtgatgtaa aagaaacctt gcgcaagttg aaagcagccc agtacaccgc tgctcaccca
      601 ggccaggttt gcccagccaa atggaacgac ggtgctgcaa cattgacacc atcattggat
      661 ttggtaggta agatctaatc tctctgagat tgacttgagc aaagccctgc gtgcagggcg
      721 ctcaggtaag gtcactcaga gatgtggctt tacctgagcg aatcccccaa ccatttttga
      781 ttgccatatg agatggccac ggctttctgt cgccgcaagg ttcagtaagt gcgtgaattc
      841 ggagaataaa aaatgttaga cgatacctta aagacacaat tgcaaaccta cttgggcatg
      901 ctgcgtcagc ctatccgtct gattgcatcc ctggataaca gtgacaacgc tgcaaaaatg
      961 cgtgaattgc tacaggaaat tactgcgttg tccgacaaag ttagcctgga tgaaagcggt
     1021 gatgacgcac gtaaaccttc attcgtgatt gccaaggaag gcgaaacacg tggcgtacgc
     1081 tttgcggcga ttccgttagg ccatgaattc acttcactag tactggcttt gctatggacc
     1141 ggcggccatc caccaaaagt ggagcaggat gtgctcgacc agattaaggc actggataac
     1201 gacttgaact ttgaagtcta tatgagcctg tcatgccata actgcccgga tgtggtccaa
     1261 gcaacgaccc tgatgaccgt gtttaatccg cgtatcaatg tcaccgtgat tgatggcggc
     1321 ctgtatcagg atgaagtcaa cgagcgtaat gtgatggccg tgccagcgac tttcctcaat
     1381 ggcgagatgt ttgcgtccgg ccgtatgctg gtggaagagt tcctggcgaa agtcgatact
     1441 ggtgcagtgg cgaaagaagc tgaaaaaatc agtgctagag aaccatttga tgtgctggtg
     1501 gttggtggtg ggccagcagg tgcttcagca gcgatttatg cggcccgtaa aggcatacgt
     1561 accgggattg tggcggatcg ttttgggggt caggtgaatg acaccctggc gattgaaaac
     1621 ttcatttccg tcaaggaaac cgaagggccg aaactggtgg cagcgctgga agagcacgtg
     1681 aaaacttatg atgttgatat catgcctttg caacgtgcag ttggcctggc tgagccatct
     1741 aaagctgata acaagcatgc cggtttgatc gaggtgaagc tggagagcgg agcaaccttg
     1801 cgcagcaagt cagtgattct ggccaccggc gcacgctgga aaaacctgaa tgtgccgggt
     1861 gaggtagagt ataaaaataa aggcgtggct tattgcccac actgtgatgg cccgttgttt
     1921 aaaggcaagc atgtcgcagt cattggcggg ggcaactccg gcgtggaagc ggcgattgac
     1981 ctggcgggtg tggttggcca tgtgacgttg ctgcagtttg ataccgagct caaggccgat
     2041 gccgtattgc aacgcaaact gcgctcactg caaaacgtga ccattctgac cagtgcccaa
     2101 accactgaaa tcaccggtga tggtacacgc gtcaatggcc tgacttacac tgatcgcgtt
     2161 agcggggaga gcaagcatgt ggcactggaa ggtgtgttta tccagattgg cctggtacca
     2221 aacagtgatt ggttgaaagg cactttgaac ttgagcaaat atggtgaaat tgaaattaac
     2281 aaccataatg ccacttcatt accaggcgtg tttgccgcag gggatgtgac cacgattcct
     2341 tacaagcaaa tcgtcattgc catgggtgaa ggctctaatg cagcactagg tgcgtttgat
     2401 tacctgattc gtcagtaagt cttttagcaa taaaaaacct gcttcggcag gttttttatt
     2461 ggaagaggtt ggttatccaa ccaaagacag tgaaacagct tcagccactt tgatgccgtc
     2521 gacagccgca gacaaaatgc caccggcata gcccgcgccc tcaccacacg ggaacaagcc
     2581 cttggtattg atgctttgta aggtgtcatc atcgcgcttg atgcggattg ggctggaggt
     2641 gcgcgtttca acacccgtca atacgccatc cgtaaggtca aaacctggca cttgcttggc
     2701 aaaggccgga atcgcttcgc gcatggcact gatggcaaat tccggcaaag cggtatctag
     2761 gttggttaag tgcacaccag gcgtataaga aggcatgact tcgccaaatt ttgtcgaagg
     2821 gcggttagcc agaaaatcac cgatcagctg ccccggggca gagtaattac tgccgccgag
     2881 tacaaacgcg ttgctctcca gttggcgctg gaatgctaca cccgccagcg gatggtcggg
     2941 gtaatccacc tcaggcgtga tgcccactac aatcccggca ttggcgttgc gctcgttgcg
     3001 gctgtattgg ctcatgccgt tagtgaccac acggttgggc tcagaggtcg ctgcgaccac
     3061 ggtgccgcca ggacacatgc aaaaactgta cacgctgcgg ccatttttgg catgatgcac
     3121 caggcggtaa tcggctgcgc ccagtttttg caggatatcg tcactatagc ttttgccata
     3181 gcgggcgcgg tcgatcaggc tttgtgggtg ctcgatacgg aaaccgattg aaaacggttt
     3241 tgcttcgaca taaatgcctt tttcatacac catctcgaag gtgtcacggg cgctgtggcc
     3301 aacggccagc accagatgtc tggtcgggat aaattcacct gtgtgcaagg tgacgccttg
     3361 tacttggtcg tgttcaatcg caatgtccgt cacgcggctt tcaaagcgga tttcaccgcc
     3421 cagcgcgatg atggttttgc gcatttcctc caccatgccc accagcctga acgtaccaat
     3481 gtgcggatgg ctgacatata agatttcttc cggagcgccg gctttgacga attcgtgtaa
     3541 taccttgcgg ctgtagtgct tggtgtcttt gatctggctg taaagtttgc catcggagaa
     3601 tgtgccggcg ccgccttcac caaactgtac gttggactca gggttcaatt cgcgcttgcg
     3661 ccataagccc caggtatctt tggtacgctt acgcacctct ttgcctcgct cgagtacgat
     3721 cgggttgaat cccgcttgcg ccagtatcag tgcagcaaaa ataccggcag ggccgaaccc
     3781 gacgaccact ggccgttcat gcgtatttgt ttcttgtctg gctacaaatt tgtagtgagt
     3841 gtctggtgca ggtttgatat gtgggtcttt tttaaacttt gccagcaatc tggcttcatt
     3901 cctgacctca acatcaatgt tatagacata caagatcgcg tgcgatttac gcgcatcaac
     3961 accacgtttg aaaatctcaa aatggattaa ttcagtctct ggaatttcca ggcgcttgag
     4021 aatggcagcc ttgatctggt catcctggtg cttgagcgtg tctgcgagct caatagggag
     4081 tttgatttca gtgattctga gcatatggag gatagtggtt atctatcaaa actctatttt
     4141 attataatca gactagtcgt atcatagatt ttgttcggct tagatttaat ctgcgcaaaa
     4201 aaatgacgat ataaacatcg tatgtttaat tacaagcgac gaatacttat cccgttaatc
     4261 attgctgctg cacttttgtg cgtatcaatg atgtggctat ttattgccat gtaccagctt
     4321 gtctattatt cagagc
//
LOCUS       DATMSK010000176         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_36837,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000176 DATMSK010000000
VERSION     DATMSK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36837"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1133
                     /locus_tag="VNZ84_05835"
     CDS             153..1133
                     /locus_tag="VNZ84_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HWU66512.1"
                     /translation="MENSTEVKPAPKLFPLIFERLHALNYSPRTQESYIQWIKRYILF
                     HSKRHPREMGAPEVEAFLNHLATEKKVSASTQNQAKSALFFLYDALEQALPALQNVTR
                     IKAQKQLPVVLSKQEVQAILSSLDGTAWLIVSLLYGSGLRVMECLRLRVADIDFARLE
                     IFVRDTNGHKNRITLLPASLVEPLKMHLQNVQALHNEDLSKGVGEVWMPPELTKKYPD
                     AGKDWQWQYIFPSSRLSVEQQSGVVYRHHADEKPIQRVVKKVAAQAGIKKVVSPNIFR
                     HSFATHLLEGGQEIKTVQQLMGHADESTTKLYTKVVNTGGRGVASPLDVI"
     gene            complement(1145..3325)
                     /locus_tag="VNZ84_05840"
     CDS             complement(1145..3325)
                     /locus_tag="VNZ84_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWU66513.1"
                     /translation="MKCPAILESEQERLAALYEHGLDDEGLIPSLDPIVQIAARMFDM
                     PVAAVNMIGSDHVFFAASVGIGETDMRRDVSFCGHAINQLEIMVVPDAAQDERFHDNP
                     LVTGDANVRFYAGVPLRSPGGLALGALCVLDNKPHLNFSEEDQRRLLELARIVSDRLE
                     LRRIETSSNRHRPAFEKYAGNSTTPIVWFDERLNIIAWNAAAALSHGYSMPEGAGMGV
                     DRLVLERDRPILQETIAKAVAADTVGHLDTFSEIFGLRKDGTKFLVGFSLFAWKEAGK
                     MRFEASMKDITEQRMEEAELRLRANVDLLTGLANRARFYRVVEDTLTVPKPAAVFMID
                     LDGFKDVNDTLGHTLGDNVLKQVASQMARLVNAPGIVARIGGDEFAIIVPHITTTEVA
                     IHLAEKIIHQIAMPILIDGQEVRVAASCGVALAPLHAQEALELVSDADLALFKAKMSG
                     RGRVCLFTPALRMEAVARRLYAMELHRAVDQGEFLLYYQPQVNLQTGAVIGAEALLRW
                     HHPKRGILTPISFMPALEGGPLAAIVGSWVLDEACAQAAYWRRHGAAEFRIGVNLFEA
                     QFRVGNLVQEVMDVLARHGLPPDALELEITENIALDNDEVVLESLRKLKSYGIGIAFD
                     DFGTGYASLSLLKTYPLTRIKIDRSFVHGMLEHKQDDSVVCATLDIAHGFDLQTIAEG
                     IEHKSQRDYLKERNCEEGQGELFGKPMPAEQFASLIGIRAASAA"
     gene            complement(3480..>3789)
                     /locus_tag="VNZ84_05845"
     CDS             complement(3480..>3789)
                     /locus_tag="VNZ84_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66514.1"
                     /translation="GILKYLETIPKEQSLWEGDCFVFDQRVAVKHGLEVGDHDQCYAC
                     RMPLTAEEMQSEQYEAGISCPYCFDKLSAEKKESLRERQKQVKLARMRGQSHIGDKQK
                     "
BASE COUNT          994 a          969 c          899 g          927 t
ORIGIN      
        1 ctctctccct gcttaggtct aacgtaaatt agaccccaaa aacgaaccta attttttatt
       61 taggctatcg tctataaaac caataaaatg atttatcata attaaatcaa tgtttagtgt
      121 atttttatta cacctattta attggatagc caatggaaaa ctcaactgaa gtaaaaccag
      181 caccaaagtt gtttccctta atctttgagc gtttgcatgc gttgaattac agcccacgta
      241 cccaggaatc ctacatacaa tggattaaac gctatatttt atttcacagc aagcggcatc
      301 cacgtgaaat gggcgcccct gaggtagaag cttttttaaa ccatttagcc actgaaaaaa
      361 aggtatcggc cagtacgcaa aaccaggcaa agagcgccct gttctttttg tatgatgcac
      421 ttgagcaagc tttacctgct ttacaaaacg tcaccagaat caaagcgcaa aagcagttac
      481 ctgtagtgct aagcaaacag gaggtacagg caattctttc cagtttagac ggcactgcat
      541 ggttaatcgt gagtttgtta tatggtagtg gcttacgggt gatggagtgc ttgcgtttac
      601 gcgtggcgga tattgatttt gccaggcttg agatttttgt gcgtgatact aatggtcata
      661 aaaaccgtat tacgctatta cctgccagct tggttgaacc attaaaaatg catttgcaaa
      721 acgtgcaagc tttacacaat gaagatttaa gcaaaggcgt tggtgaagtg tggatgccac
      781 ctgaactgac aaaaaaatat ccggatgctg ggaaagactg gcagtggcag tatatcttcc
      841 catccagcag gttgtcagta gagcagcagt ctggtgtggt gtataggcat catgccgatg
      901 aaaaacccat acaacgagtg gtgaaaaagg tagccgcgca agcaggcatc aagaaagtag
      961 tctcgcccaa catctttcgc catagttttg ccacgcactt actagaaggt gggcaagaga
     1021 ttaaaacggt acaacaactg atgggtcacg cagacgaaag tacgaccaag ctgtacacaa
     1081 aagtggtaaa tacgggcgga cgtggtgtcg ccagcccgtt agatgtcatt taacgactta
     1141 taaactacgc tgccgaagcg gcccggatgc cgattaggct ggcaaactgt tcggccggca
     1201 tgggcttgcc aaacaattca ccctgccctt cttcgcaatt tcgttccttg aggtaatcac
     1261 gctggctttt atgttcgata ccttctgcaa tggtttgcag gtcaaagccg tgcgcaatat
     1321 ccagtgtggc acagaccacg gaatcatcct gcttatgctc tagcatgcca tgcacaaacg
     1381 agcgatcaat tttaatgcgt gtgagcggat acgttttaag caggctgagt gaggcatagc
     1441 ctgtgccaaa gtcgtcaaac gcaatgccta tgccgtagct tttcagtttg cgcaggcttt
     1501 caagcacaac ttcatcatta tccagggcaa tgttttcggt gatctccagt tccaatgcat
     1561 ctggaggcaa tccatggcgt gcaagtacat ccatcacttc ctgcactagg ttgcccacgc
     1621 ggaattgcgc ttcaaacaag ttcacgccaa tacggaactc tgcagcgcca tgccgacgcc
     1681 agtaagcggc ttgcgcgcaa gcttcgtcca gaacccagga gccaacaatg gccgcgagcg
     1741 ggccgccttc gagggcaggc ataaatgaaa tgggcgtcag tatgccacgt ttgggatgat
     1801 gccagcgcaa caaggcttcg gcgccgatga cagcacccgt ttgtaaattc acttgcggct
     1861 ggtaatacag caaaaattcg ccctgatcga ccgcacgatg taactccatg gcatacaggc
     1921 ggcgcgcgac agcctccatc cgaagcgcag gggtaaacag gcagacccgg ccacggccag
     1981 acattttggc cttgaacagt gccaggtcag catcactcac caactccaat gcttcttggg
     2041 catgcaatgg cgccagcgcc acgccacagc tcgcagccac acgcacctct tgcccatcaa
     2101 tcaggatagg cattgcgatc tggtgaatga ttttttccgc caggtggatg gcgacttctg
     2161 ttgtcgtaat atgaggcacg atgatggcga actcgtcacc gcctatcctg gccacaatcc
     2221 cgggggcatt caccaaccgg gccatttgag aagcgacttg ttttaataca ttatcgccca
     2281 acgtatgacc caaggtatcg ttcacatcct taaagccgtc aaggtcaatc ataaacacgg
     2341 ccgctggttt tggtaccgtc agcgtatctt cgacaacacg gtaaaatcgc gcacggtttg
     2401 ccaggccagt cagcaagtca acattggccc gcaagcgcaa ctcagcttct tccattcgct
     2461 gctcggtaat atctttcatg ctggcttcaa accgcatttt gccagcctct ttccaggcaa
     2521 ataaggagaa accgaccagg aacttggtgc catccttgcg caggccgaaa atttcagaaa
     2581 aggtatccag atgccccacg gtatcggctg caacggcttt tgcgatggtc tcctgtagga
     2641 taggacgatc acgctcaaga accagcctat cgacgcccat ccctgcccct tctggcatac
     2701 tgtatccatg cgaaagtgcc gcggcagcat tccatgcaat gatattaaga cgctcatcaa
     2761 accatacaat tggcgtggtt gaattaccgg catacttttc aaaggctggg cggtggcggt
     2821 tgctggaggt ttctatacgc cggagctcga ggcgatcaga cacaatacgt gcaagctcca
     2881 acaggcggcg ttgatcttct tctgaaaagt ttagatgcgg cttgttatcg agcacacata
     2941 aggcgcccag ggcaagaccg ccaggagaac gcagaggaac acccgcataa aatcgtacat
     3001 ttgcgtcgcc agtgaccaag ggattgtcat ggaagcgctc gtcctgcgcg gcatctggca
     3061 ccaccataat ctctagttgg ttgatggcgt ggccgcaaaa agatacatca cgccgcatat
     3121 cggtttcacc aataccaaca ctggcagcaa aaaacacatg gtcactgcct atcatgttga
     3181 ccgcagccac cggcatatca aacatgcgtg cggcgatctg gacgataggg tccaggctgg
     3241 gaatcagccc ttcatcatct aagccgtgct catataatgc ggctaaccgt tcttgttcac
     3301 tctccaaaat ggcaggacac ttcatcgcag ctccctgtta ctagcttgga ttaatggact
     3361 gttgtgggtc aatggactgt gaattgcaag tcacccaact tcatgacata tatttacata
     3421 attatttcaa tttggcacta cgctcaagtt gcccattcgg gctattggag gagaaggtgt
     3481 tatttctgct tgtcgccaat gtggctttgg ccgcgcatcc gggccagttt gacctgtttt
     3541 tgccgctcac gcagtgactc ttttttctca gcagataatt tgtcaaaaca atatgggcaa
     3601 gaaatgcctg cttcgtattg ttcgctttgc atttcctctg cggtcaaagg catacggcaa
     3661 gcgtagcact ggtcatgatc accaacttcc aggccatgct tgaccgctac acgctggtca
     3721 aacacaaaac aatcgccttc ccacaggctt tgctctttag ggatggtttc caggtattta
     3781 agaataccg
//
LOCUS       DATMSK010000177         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_389671,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000177 DATMSK010000000
VERSION     DATMSK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389671"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VNZ84_05850"
     CDS             complement(<1..556)
                     /locus_tag="VNZ84_05850"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWU66515.1"
                     /translation="MKQVNNNISYSFEFFPPKTAEGMANLRKVRHELAAFSPEFFSVT
                     FGAGGSTRDRTMESVLEIQSEGFGAAPHISCISSSKEEIRELLQAYQSKGIKRLVTLR
                     GDIPSGEVSAGDFKYANELVGFIRAETGDWFHLEVAAYPEFHPEAGSAQKDLENFKRK
                     IDAGADSAITQYFYNVDAYFRFVDA"
     gene            complement(602..952)
                     /locus_tag="VNZ84_05855"
     CDS             complement(602..952)
                     /locus_tag="VNZ84_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWU66516.1"
                     /translation="MFKLLVVWVLNAVALISVTYLVPGIHVANFKAALVAALVIGLVN
                     VLIKPLLVILTLPITLLTLGLFILVINGLLFWAVGNYLEGFSVSSIFIGVIGALVYSL
                     ISGVLSAIVVDGKD"
     gene            complement(1041..2450)
                     /gene="ahcY"
                     /locus_tag="VNZ84_05860"
     CDS             complement(1041..2450)
                     /gene="ahcY"
                     /locus_tag="VNZ84_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWU66517.1"
                     /translation="MNTVADIKDYVVADMALADWGRKEIAIAETEMPGLMAIREEYAA
                     QQPLKGARITGSLHMTIQTAVLIETLKDLGADVRWASCNIFSTQDHAAAAIAATGTAV
                     FAVKGETLEDYWDYTHRIFEWTDGGYSNMILDDGGDATLLLHLGTRAEKDLSVVANPT
                     SEEEVCLFNAIKAKLKMDPNWYSVRLAAIKGVTEETTTGVHRLYQMHKEGKLAFPAIN
                     VNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIAVVAGYGDVGKGSAQALRALSA
                     QVWVTEIDPICALQAAMEGYRVVTMDYAAEHADIFVTATGNYHVITHEHMAKMKDQAI
                     VCNIGHFDNEIDVASIEKYQWEEIKPQVDHVIFPDGKRIILLAKGRLVNLGCGTGHPS
                     YVMSSSFANQTIAQIELFTRTSEYPVGVYTLPKHLDEKVAILQLKKLNAQLTTLTDAQ
                     AAYIGVNKNGPYKPDHYRY"
     regulatory      complement(2543..2625)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            2857..3000
                     /locus_tag="VNZ84_05865"
     CDS             2857..3000
                     /locus_tag="VNZ84_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66518.1"
                     /translation="MYQVMDEENHCIATFHHFQEAIYTAQDFTLWDDEHYYHVEELDM
                     AVA"
BASE COUNT          847 a          819 c          717 g          778 t
ORIGIN      
        1 cagcatcaac aaagcggaag taagcatcca cattgtaaaa atactgtgta atggctgaat
       61 ccgcgcccgc atcaatttta cgtttgaagt tttccaggtc tttttgtgca gaacccgctt
      121 ccgggtgaaa ttcgggatac gctgcgactt ccaggtgaaa ccagtcaccg gtttcagcac
      181 ggataaagcc cacaagttca ttggcatatt taaagtcgcc agcactgacc tcgcctgaag
      241 ggatatcgcc acgcaacgtc accaaccgtt taatgccttt ggattggtac gcttgtaata
      301 gctcacggat ttcttccttg cttgaggaga tacatgagat atgaggcgca gcgccaaacc
      361 cttcggactg gatttccagc acactttcca tggtacggtc gcgtgtagaa ccacctgcac
      421 caaatgtgac agagaaaaat tcaggtgaaa atgcagccaa ctcatgacgc accttgcgca
      481 aattggccat gccttccgcc gtttttggcg gaaagaactc aaaactgtag gagatgttat
      541 tgttcacttg tttcatcttt aatctatatt gctcttgacg ctacaccagc gggctgagta
      601 cttaatcttt accatccacg acaatcgcac tgagcacgcc cgagatcagg ctgtacacca
      661 gcgcaccaat cacaccgatg aagatagagc tgactgaaaa accttccagg taattgccca
      721 ccgcccaaaa cagcagtccg ttaatgacaa ggataaacag gcccagcgtt aataatgtaa
      781 tgggtaaggt cagaataacc agcagcggtt taatcaacac attgaccagg ccaatcacca
      841 aggctgccac caatgccgct ttaaagttcg ccacatgaat gccagggacc aaataggtga
      901 ctgatatcag cgcgactgca ttcaaaaccc agacaaccag caacttaaac atgctcactc
      961 ctcaggatgg acttgagaag agtgtacacc gaaaccaccg ccaggacatc tggcggcggc
     1021 tgaccggtga atactgccgt ttagtatctg tagtgatctg gcttgtatgg gccgttttta
     1081 ttcacgccaa tataagctgc ttgcgcatct gtcagggtgg tcaattgcgc attgagtttc
     1141 ttcaactgca agatggccac tttctcatcc aggtgcttgg gcaaggtata tacgccaact
     1201 ggatactcgc tggttcgcgt aaacaactcg atttgcgcaa tcgtctggtt cgcaaagctg
     1261 gagctcatca catagctcgg gtgaccagta ccgcaaccca ggttcaccag acggcctttt
     1321 gctaacagga tgatacgttt gccatccggg aaaatcacgt gatccacttg tggcttgatc
     1381 tcttcccatt ggtatttttc aatgcttgca acgtcgattt cgttgtcgaa atggccgata
     1441 ttacatacga tcgcctgatc tttcattttg gccatgtgtt cgtgcgtaat cacatggtag
     1501 ttaccggtgg cagtcacaaa aatgtccgca tgctcagccg cataatccat ggtcacaaca
     1561 cggtagcctt ccatcgcagc ctgcaaggcg caaatcgggt caatctcagt cacccagact
     1621 tgggcagaca aggctcgcag cgcttgcgct gaacctttac ccacgtcacc gtagccagcg
     1681 accacggcaa ttttgccggc aatcatcacg tcggtcgcac gcttgatacc gtctacaagg
     1741 gactcacggc agccatacag gttatcaaac ttggacttgg tcacggagtc attaacgtta
     1801 atcgccggga aagccagctt gccctctttg tgcatctggt acaaacggtg tacgccagtg
     1861 gtggtttctt cagtcacgcc cttgatcgca gccaaacgaa cagagtacca gtttggatcc
     1921 attttcagct tggctttgat cgcattaaac aggcagactt cttcttcaga agtcggattg
     1981 gccaccacag ataaatcttt ttcagcgcga gtacccaggt gtaacaataa agtcgcatca
     2041 ccaccatcat ccaggatcat gttggagtaa ccaccgtcag tccactcaaa aatacggtgg
     2101 gtgtaatccc agtagtcttc cagtgtttca cctttcacgg caaacacagc cgtgcctgtc
     2161 gcagcaatcg cagcggcagc gtggtcctgg gtagagaaaa tattacagga ggcccagcgc
     2221 acgtctgcgc ccaggtcttt cagcgtttca atcagcaccg ctgtctgaat ggtcatatgc
     2281 aaagaaccag tgatgcgtgc gcctttgagt ggctgttgtg ccgcgtattc ttcgcggatc
     2341 gccatcaagc caggcatttc ggtttcagcg atggcaatct ctttgcggcc ccaatcggcc
     2401 aaagccatgt cggccacaac gtagtcttta atatcagcta cagtattcat gtttcaaatc
     2461 cttaaaaaaa tgaaaactga gcaggaaaag ttctaagaac cggtggaaac gtttgttcac
     2521 cttttttccg tgctcgcaca gtgaaaagtg aacgccgttg agatgctttg cagcaaatgc
     2581 tctgagcctg ggagtcagat gactctcgca gcgttcctca gaagttgcgg attatacgcc
     2641 aaccccggca ttttacaaaa aatcggccag agccaagcgc gaaacaaggg gcgaaatcca
     2701 ccgtaaatat gctgcctaaa cgcgcagaaa acactatccc tcaaactgac acaattacac
     2761 cttgttgctc tgcaaaatca ctgtcacaca cctgtgtcat gatgtcatca cggcatcaaa
     2821 cagataacca gattaggaaa ggtggctcgt atgacaatgt atcaagtaat ggacgaagag
     2881 aatcattgca tcgcaacctt tcatcatttt caggaagcga tttataccgc acaagatttc
     2941 accctgtggg atgatgaaca ttactaccat gtagaagagc tggacatggc agtagcataa
     3001 gcgattgcac cattcgattt aagacccaag tcgatttaat acccaagtga tttaaaacaa
     3061 aaagcgcccg gttgggcgct ttttcatatt gttgattttg cttcaaattg atttttcttc
     3121 actagggcaa ctagggcagg cttttcattg ctaccgtcaa g
//
LOCUS       DATMSK010000178         7433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_107855,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000178 DATMSK010000000
VERSION     DATMSK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107855"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VNZ84_05870"
     CDS             complement(<1..392)
                     /locus_tag="VNZ84_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU66519.1"
                     /translation="MSEILRLEGLTITPTHAPERKLVSNVSFTLAQGKTTCIVGESGS
                     GKSLTALSIMQLLSPQLHMQGRVFFQGQDISQFNDAAMQHLRGRQMGMIFQEPMTSLN
                     PVLTIGFQIGEPLQTHLGLKGQALKQKV"
     gene            complement(389..1144)
                     /locus_tag="VNZ84_05875"
     CDS             complement(389..1144)
                     /locus_tag="VNZ84_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882093.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWU66520.1"
                     /translation="MSEQKHFFNAMTALGLLLAVGMASAAFILGVQAKHAVSSQQSIT
                     VKGLAEKPVKADRAQWTINIGVVAPTQAETLRAIDRERAVLANFLDQQGLDSSMRTID
                     VQTLGPHYEEEYIHDSPRQVQRGFEGYQSVHISSTDLKKIAAANQAILALRADNHPVT
                     SQPPEYLVSNLETVKMSLIADATKNARTRATEFVKQDGVKVGVMKSASQGAFYILPAT
                     GGEDSDNSYGGIYDKSTVDKIARVVVTVVYSIE"
     gene            complement(1169..2428)
                     /gene="hisS"
                     /locus_tag="VNZ84_05880"
     CDS             complement(1169..2428)
                     /gene="hisS"
                     /locus_tag="VNZ84_05880"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWU66521.1"
                     /translation="MTQKFQSIKGFYDILPEATPLWFKLEDTARRILSQYGYHNIRMP
                     VVEPTELFVRSVGEHTDIVEKEMYAWEDKLNGDKLTLRPEGTAGCVRAVIEHNLTYNG
                     PQRLWYMGPMFRHENVQKGRQRQFHQIGVEAFGFDGPDVDAEQIVLLARLWQALGIKD
                     VELQLNSIGDAEERAQYRQTLITYFEQHAELLDEDAKRRLHSNPLRILDTKNPRMQAM
                     CEAAPKLMDCLGEASRQHFARLCSLLDQAGIAYVINHRLVRGLDYYNRTVFEWVTTKL
                     GAQGTIAGGGRYDTLVERIGGSATPACGFGIGLERVFLLMQEYGVTAQNQADIYLVNV
                     GELAEQKAFTVAENLRSMGLSVVLHAGGGSFKSQMKKADRSGARFAAILGDDEAAADE
                     LSLKPMLVQGEQARVKLDQVLGIIHSS"
     gene            complement(2432..3712)
                     /gene="ispG"
                     /locus_tag="VNZ84_05885"
                     /gene_synonym="gcpE"
     CDS             complement(2432..3712)
                     /gene="ispG"
                     /locus_tag="VNZ84_05885"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWU66522.1"
                     /translation="MISRRNTRQVMVGHVAVGGGLDGTTVSPVVVQSMTNTDTADAQA
                     TIAQVYELWQAGSEVVRITVNSPEAAAQVATIRKGLDALGCPVPLVGDFHYNGHKLLQ
                     AYPDCAQALAKYRINPGNVGKGSKRDEQFAAMIEAAIQYKKAVRIGVNWGSLDQAKMA
                     QMMDDNGKSVSPLSADALMREALIQSALESAQQAVDLGLNPDQIIISCKVSNVQDLVL
                     VYQELGNRCDYPLHLGLTEAGMGSKGVVASTAALAILLQQGIGDTIRVSLTPEPTESR
                     TKEVVVAQEILQTMGIRSFTPLVTACPGCGRTTSTYFQELALQIQTWLRNQMPIWRSQ
                     YPGVETLNVAVMGCVVNGPGESKLANIGISLPGTGEVPVAPVFVDGEKTVTLKGDHIA
                     EEFQQIVDDYVHTHYAQGGKLRAAKSGVIPIVAI"
     gene            complement(3715..4938)
                     /locus_tag="VNZ84_05890"
     CDS             complement(3715..4938)
                     /locus_tag="VNZ84_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HWU66523.1"
                     /translation="MTSEDKTNVNPAEAAEKPKRGRKRKTELSPSASNQLTPTTTSEN
                     NAAPLSTSFESANSPEQQIQTPDSPEEYGAALAMESSPQPVTMALSGSGCGNVLKAAR
                     EAQGLSIHEVCSQLRLGIKQIQAIEQDDFEKLPQPSIVRGFIRNYARLLNIDVQPILE
                     AYQRIVPNKAPLPLSVRSNASPSVIDKPARAFRPQRLLTFLIFLILAGIAAYFYINHI
                     KPHALQDASLAPDVGEISDTTGQEIAMPAPESVPATPDTAPAPTATEGLDTSTATTQA
                     ADNSIQSVPGPNNETSSVEPTANNGTDSTIVSTPTPATTQAAESTTLKATDPQKANLT
                     FKVTEDSWVRIEDQQGKKVFSEVLPAGSERQVITEKPVNITVGHAAGTQLTIDNQPYD
                     LTQATRGRVARIQLK"
     gene            complement(4945..5742)
                     /gene="pilW"
                     /locus_tag="VNZ84_05895"
     CDS             complement(4945..5742)
                     /gene="pilW"
                     /locus_tag="VNZ84_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HWU66524.1"
                     /translation="MKSTFFAPSAVMLALATALWLNGCAQNPSASNNSPYDQQAIGRE
                     TGDVESARVHTELGAEYFRLKRYEVALEEFNTAIERSPSYALAYNGLGLVYATLGEQA
                     RAEEAFKRSIQLQPSNSESHNNYGRFLCDQGKFEASQKEFALAVKNPLYKTPQVAFYN
                     AGVCAMRAQQNKQAENFFFQALQVDPLSHASAYQLANLQFSRGEPALALNTLQNSVNV
                     APTPESLFLAVRICRALHMSDDATYYSVQLHKLFPNANETKQLLKME"
     gene            complement(5746..6864)
                     /gene="rlmN"
                     /locus_tag="VNZ84_05900"
     CDS             complement(5746..6864)
                     /gene="rlmN"
                     /locus_tag="VNZ84_05900"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWU66525.1"
                     /translation="MVNLLNLNQPQLAEYFLSLGEKPFRAKQLMRWMHHFGVLDLEQM
                     TDIAKTLREKLRQETEFTLPDVQLEQVSDDGTRKWLIGTNNLRDGTANSVETVFIPED
                     DRGTLCISSQVGCALECTFCSTGRQGFNRNLQVSEIIGQLWIANHALRQAPGYDLLPP
                     GERIISNVVMMGMGEPLANYDNVVTAMQIMLDDSAYGLSRRRVTLSTSGMVPAMDRLK
                     EDCPVALAVSLHAPNDALRDEIVPINKKYPIKELMAACERYLVKAPRDFVTFEYVMLD
                     GVNDTLEHAKELINIVRNVPCKFNLIPFNPFPNSGYGTSKPMQVRAFRDMLMQAGFVV
                     TVRKTRGDDIDAACGQLAGKVLDKTKRSEKTIAIHPEQ"
     gene            complement(6915..7319)
                     /gene="ndk"
                     /locus_tag="VNZ84_05905"
     CDS             complement(6915..7319)
                     /gene="ndk"
                     /locus_tag="VNZ84_05905"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWU66526.1"
                     /translation="MALERTLSIIKPDAVAKNVIGQIYTRFENAGLKIVAAKMTQLTQ
                     AEAEGFYAVHKERPFFNDLVKFMISGPVMIQALEGENAVLKNRELMGATNPKDAAPGT
                     IRADFADSIDANAVHGSDSLENAAIEIKYFFG"
BASE COUNT         1661 a         1866 c         1933 g         1973 t
ORIGIN      
        1 tcgacctttt gtttgagcgc ctggccttta aggccaagat gggtttgtaa tggctcgccg
       61 atttgaaagc cgatggtaag tactggattc aaggaggtca tgggttcctg gaaaatcata
      121 cccatttgcc tgccacgcag atgctgcatc gcagcatcgt taaattggct gatatcttgc
      181 ccctggaaga acacgcgtcc ttgcatgtgc aattgtggcg acaacagttg catgatactg
      241 agcgctgtca ggctttttcc gctaccagac tcaccaacga tacaggtggt cttgccctgt
      301 gctaatgtga atgagacatt gctgaccagt ttacgctccg gtgcatgtgt tggcgtaatg
      361 gtcagtcctt ctaaacgcag gatttcactc attctatgct gtacaccacg gtgaccacta
      421 cccgggcaat tttatcaacg gtggatttat cataaatgcc gccatagctg ttatcactgt
      481 cttcgccacc agtagcaggc aggatataaa atgcaccctg gctggcagac ttcatgacgc
      541 cgaccttgac gccatcctgt ttgacaaatt cggtggcacg ggtacgcgca tttttggtag
      601 cgtctgcaat cagtgacatt ttgacggttt caaggttgct caccaggtat tcaggtggtt
      661 gcgaggtgac gggatggttg tcggcacgca gggctaaaat cgcctggttg gcggctgcaa
      721 tttttttgag gtcggtggag ctgatatgca cgctttggta gccttcaaaa ccgcgctgga
      781 cctggcgagg gctatcatgg atgtattctt cttcataatg cgggcccagg gtttgtacat
      841 cgatggtgcg catgctggag tccaggcctt gctggtctag gaaatttgcg aggactgccc
      901 gttcgcggtc gatagcacgt agcgtttcgg cttgggtagg agcaaccaca ccaatattaa
      961 tcgtccattg cgcacggtcc gccttgactg gtttttctgc caagcctttg acggtaatgg
     1021 actgttggct ggagaccgca tgtttggcct gcacgccaag aataaaggcg gcagatgcca
     1081 tcccgactgc cagtaacagg cccagggccg tcatggcatt aaaaaaatgt ttttgctctg
     1141 acatccggcc tcctgtcatc aatgacaatt aacttgaatg aatgatgcct aatacctggt
     1201 caagtttaac acgtgcttgc tcgccttgga ccagcatggg tttcaggctg agttcatctg
     1261 cggcggcttc atcatcaccc aaaatggcag caaacctggc gccactgcgg tctgcttttt
     1321 tcatttgtga tttaaaactg ccaccccctg catgtaatac gacactgagt cccatgctac
     1381 ggaggttttc tgcgaccgta aacgctttct gttctgctag ttcaccaacg ttcaccaggt
     1441 aaatgtcggc ctggttttgt gcggtgacgc catattcctg catcagcaag aatacacgct
     1501 ccaagccaat gccaaagccg caggccgggg tggcgctgcc gccaatgcgt tcaaccaagg
     1561 tgtcataacg gccaccaccg gcaatggtcc cctgggcgcc gagtttggtg gtcacccatt
     1621 caaacaccgt acggttgtaa taatccagcc cgcgtaccag tctatggtta atcacataag
     1681 caatgcccgc ctggtcaagt aggctgcaca agcgcgcaaa gtgttgtctg gaagcctcac
     1741 cgaggcagtc catcagttta ggcgctgctt cgcacatggc ctgcatgcgt ggatttttgg
     1801 tgtctaggat acgcaagggg ttactgtgca ggcggcgctt ggcatcctca tccagcaatt
     1861 ctgcatgctg ttcaaaataa gtaatcaagg tctggcggta ctgcgcacgt tcttccgcat
     1921 caccgatgct gtttaactgt aattcaacat ctttaatgcc aagcgcctgc cacaaacggg
     1981 ccaacaggac aatttgctcg gcatccacat ctggtccatc aaagccaaac gcttccacgc
     2041 caatctggtg aaattggcgc tggcggcctt tctgcacgtt ttcatggcgg aacatcgggc
     2101 ccatatacca caagcgttgt ggaccattgt aggtcaggtt atgctcaatg actgcacgca
     2161 cacagcccgc agtaccttcc gggcgcaggg tcagtttgtc gccattgagt ttgtcttccc
     2221 aggcatacat ttctttttca acaatatcgg tatgctcacc cacactgcgt acaaacaatt
     2281 cggtgggttc taccaccggc atgcgaatat tgtgataacc atactggctc aaaattctgc
     2341 gtgcagtatc ttcgagttta aaccagagtg gggtggcttc aggcaggatg tcgtaaaagc
     2401 ctttaatgga ttgaaatttt tgcgtcataa actaaatagc aacgatagga attacgccag
     2461 atttggcagc gcgtagtttg ccgccttgag cgtagtgcgt atggacgtaa tcgtccacga
     2521 tctgttgaaa ctcttcggca atatggtcgc ctttgagggt aaccgttttt tcgccatcga
     2581 caaagactgg cgccaccggg acttctcccg tacccggcag gctgatacca atgttggcca
     2641 gtttggattc acctggtcca ttcaccacac agcccatgac agctacgttg agtgtttcta
     2701 ccccgggata ttggctgcgc cagattggca tctggttgcg caaccaggtt tggatttgta
     2761 atgccaattc ctggaaatag gtactcgttg tgcgtccgca gccaggacaa gcagtcacca
     2821 aaggtgtgaa ggagcggata cccatggtct gcaggatttc ctgcgccacc acgacttctt
     2881 tagtacggga ttcggtgggt tctggcgtca gggagacgcg tatggtatcg cctatgcctt
     2941 gctgtagcag aatggcaagt gcagcggtag acgccaccac gcctttagag cccatgcctg
     3001 cttcggtcag gcctaagtgc aaggggtaat cacaacggtt accaagctcc tggtagacta
     3061 gcaccaggtc ctgcacattg ctgaccttgc acgaaataat gatttgatca ggatttaaac
     3121 ccagatccac ggcttgctgg gcactttcca gcgccgactg aatgagcgct tcacgcataa
     3181 gggcatcggc agacagcggg ctgactgatt taccgttatc atccatcatc tgcgccattt
     3241 tggcctgatc caggctaccc cagttcacgc ctatgcgcac ggctttttta tattggatag
     3301 cagcttcaat catggcggca aactgttcgt cgcgcttgct gcctttgccg acattccccg
     3361 ggttaatgcg gtatttagct aatgcttgtg cgcaatcggg ataggcttgc aatagcttgt
     3421 gaccgttata gtgaaagtca cccaccagtg gtaccgggca acctagtgca tccagccctt
     3481 tgcggatagt cgctacctgg gctgctgctt ccgggctgtt tactgtaatg cgtaccactt
     3541 cggagccggc ctgccataat tcataaactt gcgcgatagt ggcttgtgca tcagcagtgt
     3601 cggtattggt catgctctgc acaacaacgg gagataccgt tgtgccatcc aggccgccac
     3661 ctacggcaac gtggccgacc atcacttgac gggtatttct acgggatatc atgattattt
     3721 caattgaatg cgtgccacac ggccacgggt agcctgtgtt aaatcataag gctgattatc
     3781 aatggttaac tgtgtacccg cagcatgtcc cacggtaata ttcacgggtt tttcagttat
     3841 cacctggcgc tcagaaccag ccggcagcac ttcactgaat acttttttgc cttgctgatc
     3901 ctcaatgcgt acccaagagt cctcagtcac tttgaatgtc aggttggctt tttgtggatc
     3961 agttgcttta agggttgtgc tttccgctgc ctgagttgtt gccggtgttg gcgtgcttac
     4021 aatcgtgcta tcagtgccat tgttggctgt aggctcgacg gaagatgttt cattattcgg
     4081 gccgggcacg gattgaatgc tattatcagc cgcctgggtt gttgctgtac ttgtgtctag
     4141 cccctctgtc gcagttggcg caggagccgt gtcaggcgtt gctggcacgc tttcaggggc
     4201 tggcatggca atttcctgac cagtggtgtc gctgatttcg cccacatctg gggccaggga
     4261 tgcatcttgc agcgcatgag gcttgatgtg attgatataa aaatatgccg caatgcctgc
     4321 caggatcagg aaaatcaaaa acgttaacag ccgttgcggg cggaacgcac gcgctggttt
     4381 atcaatcacc gatgggctag cattggaacg aacgcttaat ggcagtgggg ccttgttggg
     4441 gacaatgcgc tggtatgctt ccaggatggg ctgcacatca atattcaaca agcgagcata
     4501 attgcggata aacccacgca cgatactggg ttgtggcaat ttttcgaaat catcctgttc
     4561 aattgcctgg atttgcttga tgcccaatct tagttggcta cacacttcat ggatactcag
     4621 cccctgggct tcgcgtgctg ctttcaggac attaccgcag cctgagcctg aaagggccat
     4681 cgtgaccggt tggggcgaac tttccatcgc caaggctgcg ccatattctt cagggctatc
     4741 tggcgtttga atctgttgtt ccggtgaatt ggcggattca aacgaggttg ataggggggc
     4801 agcgttattt tcgctagtcg ttgtaggcgt caactgattc gaagcagaag gagaaagctc
     4861 cgttttgcgc ttacggccac gttttggttt ctctgctgcc tctgcaggat tgacgttggt
     4921 tttgtcttct gacgtcactc gcttttactc cattttcagc aattgtttgg tttcgttggc
     4981 gttagggaac agcttatgca attgaacgct gtagtaggtc gcatcatcgg acatatgtaa
     5041 tgcccggcaa atgcgtactg ccaaaaatag tgactccggt gtcggcgcaa cgttcacgct
     5101 gttttgtagg gtattcaagg ccagcgctgg ctcgccacga ctaaattgca ggttggctag
     5161 ctggtaagcg ctggcatgtg agagagggtc tacttgcaag gcctggaaaa agaaattttc
     5221 tgcctgctta ttttgctgtg cgcgcatggc acagaccccg gcattataaa acgctacttg
     5281 gggcgtcttg tacaaaggat tcttgaccgc caacgcaaac tctttttgtg aggcctcaaa
     5341 cttaccctgg tcgcataaaa agcggccata gttattgtgc gactcggagt ttgatggttg
     5401 cagttgtatg gaacgtttaa acgcctcttc agcgcgggct tgctcaccta gggtcgcata
     5461 taccagcccc aggccattat aggcgagggc atagcttggg ctgcgctcaa tcgctgtgtt
     5521 gaattcttcc agagcgactt cataccgttt gaggcggaaa tattcagctc ctaactcagt
     5581 atgcacgcgc gcggattcaa cgtctccagt ttcgcgacca atggcttgct ggtcgtaagg
     5641 agagttgtta cttgctgacg gattttgcgc acaaccattc agccacaacg ctgtggccaa
     5701 ggcaagcatg accgcggatg gcgcgaagaa ggttgatttc atgacttact gttccggatg
     5761 aatcgcaatg gttttttctg accgtttagt tttatctaag accttgccag caagctggcc
     5821 acaggctgca tcaatatcgt cgccgcgtgt tttacgcacg gtcaccacaa agccagcttg
     5881 cattaacata tcacgaaatg cgcgcacctg catgggtttg gacgtgccgt aaccactgtt
     5941 ggggaagggg ttgaatggga tcaggttaaa tttgcacggg acattgcgca caatattaat
     6001 cagctctttg gcgtgctcca aggtgtcatt cacaccgtcc agcatcacgt attcaaaggt
     6061 cacgaaatca cgtggggcct tgaccaggta gcgctcacag gcagccatca gctctttaat
     6121 cgggtatttt ttgttgatgg gtacaatttc atcacgcagg gcatcattag gcgcatgcaa
     6181 tgaaacagcc aatgctaccg gacaatcctc tttcaagcgg tccatggcag gcaccatgcc
     6241 actggtcgat aaggtcacgc gacgccgact caggccgtaa gcgctgtcat ccagcataat
     6301 ctgcatggcc gtgacgacgt tgtcatagtt cgccaatggc tcacccatgc ccatcatgac
     6361 cacgttggaa atgatgcgct caccgggtgg cagcaagtcg taacctggtg cctggcgtaa
     6421 ggcgtggttg gcaatccata actggccgat gatttcggag acttgcagat tgcggttaaa
     6481 gccctggcgg cccgtgctgc agaatgtgca ttccagcgca cagcctacct ggctggagat
     6541 acataatgta ccgcggtcat cttcagggat aaaaacagtt tctacactgt tggcagtgcc
     6601 atccctaagg ttgttggtcc caatgagcca cttccgagtg ccatcgtcag acacttgttc
     6661 cagttgcaca tctggcaggg taaattctgt ttcctggcgt agtttctcgc gcagggtttt
     6721 ggcaatatcg gtcatctgtt cgaggtctaa caccccgaag tgatgcatcc agcgcatcaa
     6781 ttgcttggcg cgaaaaggct tctcgccgag acttaaaaag tactcggcga gttgcggttg
     6841 gttaagattc aataaattaa ccaagtgtat tccttaagct ttggaatcat tcatcagttg
     6901 agtggatgaa gcgattagcc gaagaagtat ttgatttcga tcgctgcgtt ttccagtgag
     6961 tcggaaccgt gcaccgcgtt cgcatcgatg ctgtcagcaa agtcagcacg gatagtgcct
     7021 ggcgctgcgt ctttagggtt agttgcaccc atcagttcac ggtttttcaa cacggcgttt
     7081 tcgccttcca atgcttgaat catcactgga ccggagatca tgaatttcac caggtcgttg
     7141 aagaaaggac gctctttgtg cacagcgtaa aagccttcag cttcagcttg tgtcagttgt
     7201 gtcattttag ctgcaacgat tttcaaacca gcattttcaa aacgggtgta gatttgacca
     7261 atcacgtttt ttgcaactgc gtctggtttg atgatgctga gtgtgcgctc taaagccatg
     7321 agaactccaa aataaaagta attaacaaga aaaacaaacg actattctac tatttttaac
     7381 gtgattgata aaccgcattc atgcacctaa gtgatgacgc gtgtcagttt att
//
LOCUS       DATMSK010000179         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_61417,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000179 DATMSK010000000
VERSION     DATMSK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61417"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VNZ84_05910"
     CDS             <1..507
                     /locus_tag="VNZ84_05910"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWU66527.1"
                     /translation="PTTIITSAHAAKQNIMAAAWVCALDFKPPKVTVVIDSKTYTREL
                     MEAEGTFAINLPCVKQIEMVKQVGGISGRDLAGTDKFAEYGIETFAAQEISAPLVKDC
                     VAWLECKIIPEPHNQNAYDLFIAEVVAAHADERVFSDGRWHFEGHDDLRTIHHIAGGV
                     FFATGQAC"
     gene            813..3008
                     /locus_tag="VNZ84_05915"
     CDS             813..3008
                     /locus_tag="VNZ84_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HWU66528.1"
                     /translation="MRHKKTDQNDQLYILLISMHGLIRGDQLELGVDADTGGQCTYVL
                     ELTKALAKQSQVAKVDLLTRLIDDPHVSTDYAQPEEVLSSKARIIRLPCGPKRYMRKE
                     SLWPHLDQMVDKCLHYLRQQRRLPDVIHSHYADAGYVGRQLSMLLGIPLVHTGHSLGL
                     PKRERLLSSGRKATTIEKQFNFERRIAEEEALLRDAALIVTSTRQEIELQYGMYAHMD
                     GKRFKVIPPGTDTTRFSPAGRRPIASGVELSITRFLEQPKKPMILCICRPELRKNING
                     LLKAYGSHPALHERANLVLVAGSREDIRQLDKAQQSVLNELLLDIDAYDLWGKVAIPK
                     HLPQEDIPELYRMAAKSGGVFINAAFTEPFGLTLLEAAASGLPIIAPDDGGPRDILAN
                     CHNGLLTNTLDSDAIAESLRSALSDKKRWQKWARNGVLNIKRHYTWDGHVTKYLKEVR
                     HLQHKHRKNLRKQRIALHEGTSTIPLADYAFISDIDNTLLGNRAAQADLVDWLNTNAG
                     EAVFGIATGRPLESAIDILRKHRIPIPDVLITSVGSEIHYGARQTPDIGWANHIRHLW
                     RREDLLQVLQALPGLRLQAPENQREFKISFLVNPEDMPGIEAIKQHLHQHHLHAQVIY
                     SHDEFLDILPVRASKGHAIRYLAYKWGLPLTRFLVAGDSGNDIEMLSGDTMAVVVGNH
                     SHELASLKGQQKIYFAQAHYAHGILEGMAHYHFGKPPSRAANAKDAVVA"
     gene            3036..>3220
                     /locus_tag="VNZ84_05920"
     CDS             3036..>3220
                     /locus_tag="VNZ84_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66529.1"
                     /translation="MYEQISHAILNDTLRKLTTGSRDKEWQYFYTRLGANFYAIYSLF
                     HHLYGHLPDFKPQLAHL"
BASE COUNT          809 a          819 c          807 g          785 t
ORIGIN      
        1 ccgactacca tcattacgtc tgcccatgct gcaaaacaaa acatcatggc tgccgcctgg
       61 gtctgcgctt tagacttcaa gccacccaaa gtcacggtcg tgattgacag caaaacctac
      121 acccgcgaac tgatggaagc ggaagggact tttgccatca acctgccctg tgtcaaacaa
      181 atcgagatgg tgaaacaagt cggtgggatc agcggccgtg atttggcagg caccgacaaa
      241 tttgctgagt acggcattga gacctttgct gcacaggaaa tcagcgcacc gctggtgaaa
      301 gattgcgtgg cctggctgga atgtaaaatt atccccgagc cgcacaacca gaacgcttac
      361 gatttgttta ttgctgaagt ggtggccgcg catgccgatg agcgggtgtt tagcgatggc
      421 aggtggcatt ttgaggggca cgatgattta cggacgatcc accacattgc tggtggtgtg
      481 ttttttgcca ctggccaagc ctgttagtta acctctgttg cgataaaaaa gccctgtcat
      541 ctttgcaggg cttttttatt gtcccgattt tgcagtgaaa ctttcctctt ttcattggtg
      601 tcaatattaa tatctgtaag caaaaagagg ggcttacagg taaatattcc accagtttcc
      661 tatacggaaa aaatcaggat gcccaggcat cttgtctatc gtgcgtgttc catcattggc
      721 cgcatgcgag tgtttcatga tcggcagtca caaaagccgg accgtttgtc ctttgctggc
      781 gttgctgccc gagaatgcag aagggaggcg ccatgcgcca taaaaaaaca gatcaaaacg
      841 accagttgta tattttgctt atcagcatgc atggcctgat ccgcggcgac cagcttgagt
      901 taggcgtaga tgcggacacc ggtggccaat gcacctatgt gctggaactg acaaaagctt
      961 tggcaaaaca atcgcaagtc gccaaggtag acttgctcac ccgcctgatt gacgatcctc
     1021 acgtctctac cgactacgca caaccggaag aagtactctc cagcaaagcc agaattattc
     1081 gcctgccgtg cgggccaaaa cgctacatgc gtaaagaaag cttatggccg cacctggacc
     1141 aaatggtgga taaatgcttg cactacttac gccagcagcg acgtttgcct gatgtgattc
     1201 actcccatta tgcggatgca ggctatgttg gccgccagct ctccatgttg ttggggatcc
     1261 cattggtaca taccggccac tcgctggggt tgccgaaacg cgaacgtttg ctctctagcg
     1321 gacgtaaagc gaccaccatc gaaaaacaat ttaactttga acggcgtatt gcagaagaag
     1381 aagcattgct gcgggatgct gcgctcatcg tcaccagtac gcgccaggaa attgagttgc
     1441 agtatggcat gtatgcccac atggatggca aacgcttcaa agtcatcccg cctggtacag
     1501 acaccacgcg tttttcgccg gcaggccgtc ggcctattgc cagtggtgtg gagctcagta
     1561 tcacccgctt tttggaacag cctaaaaagc caatgatatt gtgtatttgc cgccccgagt
     1621 tgcgcaaaaa cattaacggt ttactcaaag cttatggatc acatccagcg ttgcacgaac
     1681 gggcgaacct ggtgctggtc gcaggatctc gtgaggatat ccgtcaattg gataaagccc
     1741 agcaatcagt attaaatgag ctgctactgg atattgatgc ttatgatttg tggggcaaag
     1801 ttgcgattcc caagcatctg ccacaggagg atatccctga gctctaccgt atggccgcga
     1861 aaagtggggg tgtttttatc aatgccgcgt ttaccgaacc ttttggcctg acgctattgg
     1921 aagccgccgc cagcggttta cctatcattg cgcccgatga tggtggtcca cgcgatattc
     1981 ttgccaactg ccacaacggc ctgctgacca acaccttgga cagcgatgcc atcgccgaga
     2041 gtttgcggag cgccctgtct gacaaaaagc gctggcagaa atgggcacgt aacggcgtac
     2101 tcaatattaa gcgccactac acctgggatg gccatgtcac caagtacctg aaagaggtac
     2161 gtcatttgca acataaacac cgcaagaact tacgtaaaca acgcatcgca ctccatgaag
     2221 gaacgtctac gattccattg gcggattatg cgttcatcag tgatattgat aacaccttgc
     2281 tgggtaaccg tgctgcgcag gctgatttag tggactggtt aaataccaac gccggtgagg
     2341 cggtttttgg cattgccaca ggcaggccac tggaaagtgc gattgatatt ttgcgcaaac
     2401 accgtatccc gattcctgat gtcctgatta catctgtcgg ttctgaaatt cactatggcg
     2461 cccgccagac gccagatatt ggctgggcga accatatccg tcacttatgg cggcgggaag
     2521 atctgctgca ggtgttgcaa gcattacctg gcttacgcct gcaggcacca gaaaaccagc
     2581 gtgaatttaa aatttccttc ctggtgaatc cggaggatat gcctgggatc gaagcgatta
     2641 aacagcactt gcatcaacat catctgcatg cacaggtcat ttactcacat gatgaattcc
     2701 tggatatttt gcctgtgcgc gcttccaaag gtcacgccat ccgctatctg gcttacaagt
     2761 ggggattgcc gctcacccgg tttttagtcg caggcgactc cggcaatgat attgaaatgc
     2821 tctctggcga cacgatggcc gtggtagtcg gcaatcatag ccatgagctt gcctcgctca
     2881 aggggcagca aaaaatttac tttgcccagg cgcactatgc acatggcatt ctagaaggca
     2941 tggcccatta ccactttggc aaaccgccct cgcgtgcggc taatgccaaa gatgcagtag
     3001 tcgcttaatc aatattcgtt ggactggaaa accgtatgta cgaacaaata tcacacgcga
     3061 tattgaatga caccttacgc aagctcacca cgggcagccg tgataaggaa tggcaatatt
     3121 tttatacccg gttgggcgca aacttttatg caatttattc gttatttcat catttgtatg
     3181 gccatctgcc agatttcaaa ccgcaactgg cacatttaat
//
LOCUS       DATMSK010000180         6985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_225160,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000180 DATMSK010000000
VERSION     DATMSK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225160"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VNZ84_05925"
     CDS             <1..1191
                     /locus_tag="VNZ84_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWU66530.1"
                     /translation="VSGLTGAIIAFEDELDAWLNPQLFRPAWQPGKPVLDPYQLQALA
                     QQQAPVGTLVDEVLFKLPEAQAASGLTERVLAFRLSPSDPRVTETVADQLFIDPQTGA
                     SLGARKYGESLFQRETLITFLYRLHYSLALPGKSGHLIMGIVALLWTLDTFVAFALTL
                     PRRQAQAKTRFWHRWRPAWLVKWQASATRINFDLHRALGLWLCTVLLIFAWSSVMFNL
                     RDQVYLPLMSKVLPFDLSWRSVPKLDQPLDRLPMPWPDAHHMARQAMQGFAQYHGLKI
                     DFEDRLRLDRVRGVYMYSVHSSADLREDRGNTAILIDAQTGDLKGWWLPTRGETGNTV
                     SNWLGALHMGQVFGLPYRIFVCLTGLALVAISVTGVLIWSRKRAGKTKLKPVGRKAVK
                     RVSV"
     gene            complement(1193..2458)
                     /locus_tag="VNZ84_05930"
     CDS             complement(1193..2458)
                     /locus_tag="VNZ84_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HWU66531.1"
                     /translation="MISTRLFSLPFATALAFAGASGAVHATNGINLIGFGAESTLMGG
                     ADVAVARDTSALNTNPAGLTQIKSPLMDVFGSVLRTTDLVHKDPRNEEHASNRYTFLG
                     GGGYARPLEGTDCTAGIGLFAQGGAGGVFENLRTPFGNRDDLSSLFGIAKIIPGIGCQ
                     VNEKLSLGASLNLVYGSIEQDFFSNTSVGTTFAGYKLEGASAIRTGFKLGMQYRVTPS
                     LTLAAAYTEKTELPMTGGTLTADFTGAGLGKVKYADASVKGFALPREVAVGLAFKPTD
                     DWLLSFKLNWLNWDDAINDVKLRATKPDNAAAPAVYGSSNPADWKNQWVVATGLAYKW
                     DDKTTLYAGHNYGKNPIPRQNSSPLLAGILEHHLTFGAARKLDQNWLMTSGVEWMLPV
                     SVKYNSPLFGDSEIRNEAIFLHFMLSRRW"
     regulatory      2809..3031
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3166..5445
                     /gene="nrdA"
                     /locus_tag="VNZ84_05935"
     CDS             3166..5445
                     /gene="nrdA"
                     /locus_tag="VNZ84_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HWU66532.1"
                     /translation="MSQYESMQATKRDGRKEPINLDKIHRVIDWAAQGLNNVSVSQVE
                     LRSHIQFYDGIRTDDIHETIIKSAADLISEETPDYQYLAARLSIFHLRKIAYGQFEPP
                     HLFDHVTNLVELGKYDKHLLSDYSREEFDVLNGYIDHWRDLNFSYAAVKQLEGKYLVQ
                     NRVTKKIYESPQFLYILVAMSLFANYKGQTRLDLIKRFYDAVSTFKISLPTPIMAGVR
                     TPTRQFSSCVLIECDDSLDSINATSSAIVKYVSQRAGIGVNAGRIRALGSEIRGGEAQ
                     HTGCLPFYKLFQSAVKSCSQGGVRGGAATLFYPLWHLEVESLLVLKNNRGVEENRVRH
                     LDYGVQINRLMYQRLIKGQNITLFSPHDVPGMYDAFFADQDEFERLYTQYEADESIRK
                     RTMPAVDLFSLMMQERAGTGRIYIQNVDHCNTHSPFDPAVAPVRQSNLCMEIALPTHP
                     LNDINDEEGEIALCTLSAFNLGALESLDELEGLADLVVRALDALLEYQDYPVKAALNA
                     TNKRRSLGVGVINYAYYLAKNGTGYTEDAALGLTHRTFEAIQYYLLKASVQLAREFGP
                     CGAFNETTYAKGILPIDTYKKDLDAVCSEPLLLDWESLRTEIQKDGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVSVKQSKDGILRQVVPEIERLRDQYQLLWKMPNNDGYLK
                     LVGVMQKFVDQSISANTNYDPTRFEGGRVPMKQLLKDLLQVYKLGIKTLYYHNTRDGA
                     TEGGTEAEDDGCASGACKI"
     gene            5644..6777
                     /gene="nrdB"
                     /locus_tag="VNZ84_05940"
     CDS             5644..6777
                     /gene="nrdB"
                     /locus_tag="VNZ84_05940"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HWU66533.1"
                     /translation="MAYSIFTKTDNDQLKEPMFFGQPVNVARYDQQKYPVFEKLIEKQ
                     LSFFWRPEEVDVSQDRADYQGLPEHEKHIFLSNLKYQTLLDSIQGRSPNVALLPLVSL
                     PELETWIETWAFSETIHSRSYTHIIRNIVNDPSIIFDDIVRNDNITARASDIAHYYDE
                     LIQQTMLYSQLGEGNHVINGETKVVNRRELKKLLYRCLMNVNILEAIRFYVSFACSFA
                     FAERKVMEGNAKIIRLIARDEALHLTGTQHILNIMRSGQDDPEFAEIAAELHNECFEM
                     FKTAAEQEKQWAEYLFKDGSMIGLNKDILSQYVEYITNSRMASVGLPPAFPNAKNNPI
                     PWINTWLASDTVQVAPQEVELSSYLIGQIDSEVSADDLNDFEL"
BASE COUNT         1680 a         1839 c         1838 g         1628 t
ORIGIN      
        1 gtctccgggt taactggcgc cattatcgcg tttgaggatg agctggatgc ctggttaaac
       61 ccgcaactgt tccggccagc ctggcagcct ggcaagcctg tgctcgatcc ttaccagcta
      121 caggcgctgg cgcagcagca agcgccagtc ggcacgctgg tggatgaagt gttattcaag
      181 ttgccagaag cccaggcagc ttctggcctg actgaacgtg tactggcctt ccggctcagc
      241 ccgtctgatc cacgcgtcac agaaacggtg gccgaccagc tgtttataga cccgcaaacg
      301 ggtgccagtc ttggggcgcg taaatatggc gagagcctgt ttcagcggga gaccttgatc
      361 acctttctct acaggctgca ttattcactg gctttaccgg gcaaaagtgg gcacctgatt
      421 atgggcattg tggcgctgtt atggacgctg gatacctttg tagcgtttgc cttgacgctg
      481 ccccggaggc aggcacaggc caaaacccgc ttctggcatc gctggcgacc ggcctggctg
      541 gtcaagtggc aggccagtgc tactcgcatc aactttgacc tgcaccgtgc attgggcctg
      601 tggctatgca cggtgttact catatttgcc tggtccagcg tcatgttcaa cttgcgtgac
      661 caggtctacc tgcctttgat gtccaaagta ttgccgtttg acctgtcctg gcgcagtgtg
      721 cccaagctgg accagccact ggaccgcttg cccatgccat ggccagacgc gcatcacatg
      781 gcacggcagg ccatgcaagg ctttgcacaa taccatggtt taaagatcga ttttgaagac
      841 aggttgcgct tagaccgcgt gcgcggcgtc tatatgtatt cagtgcatag ttctgccgac
      901 ttgcgtgaag accggggcaa tacggcgatc ttgattgatg ctcagaccgg ggatctgaaa
      961 ggctggtggc taccgaccag gggtgaaaca ggcaataccg tcagtaactg gctgggggcc
     1021 ttgcatatgg ggcaggtgtt tggcctgcct taccggatat ttgtctgttt gactggcctg
     1081 gctctggttg ccatttccgt cacgggggtg ttgatctgga gccgcaagcg tgcaggcaaa
     1141 acaaagctta agcccgttgg tcgcaaagcg gtaaaacgtg tttcagtcta atttaccagc
     1201 ggcggcttag cataaagtgc aggaaaatgg cttcgttgcg gatttctgag tcaccaaaca
     1261 atggactgtt gtatttgacg ctgactggta gcatccactc cacaccactt gtcatcagcc
     1321 agttctggtc tagtttgcgc gccgcaccaa aagtcaggtg atgttccagg atgcctgcca
     1381 gtaatgggct ggaattctgg cgcgggatcg ggtttttgcc atagttatgg cccgcataaa
     1441 gtgtcgtctt gtcatcccat ttataggcaa gccctgtcgc gacgacccac tggtttttcc
     1501 agtctgcagg attgcttgag ccatacacag ccggtgctgc agcattgtca ggttttgtgg
     1561 cacgcagttt tacgtcatta attgcatcgt cccaattaag ccaattgagc ttgaatgaca
     1621 gtagccagtc atctgtaggc ttgaatgcca accctaccgc cacttcgcga ggcaaggcaa
     1681 agcctttgac tgaagcgtca gcatacttca ccttacccaa tccggcgccg gtgaagtcgg
     1741 ccgttagcgt gccgccagtc atgggaagct cggttttttc ggtataggca gccgccagcg
     1801 ttagcgaagg cgtcacccga tattgcatgc cgagcttgaa gcccgtacgg atagccgaag
     1861 caccttccag cttgtagcca gcaaaggttg tgccgactga agtgttggaa aagaaatcct
     1921 gctctatgct gccatacacc agattcaggc tggcgcccaa tgaaagcttt tcgttcacct
     1981 ggcagccgat gccgggaata atcttggcga taccaaacag agaggacaaa tcatcacgat
     2041 tgccaaacgg tgtgcgcaaa ttctcgaaca cgccacccgc gccaccttgg gcgaacaagc
     2101 ctataccagc cgtacaatcg gtaccctcca gtggccgtgc gtagccgcca ccacccaaaa
     2161 aagtatagcg gttagaggcg tgctcttcgt tacgagggtc tttatgcaca aggtccgtcg
     2221 tgcgcagcac agagccaaac acatccatga gcggcgactt gatttgtgtc agcccggccg
     2281 ggttggtgtt cagcgcactg gtatcgcgcg ccacggcgac atcggcgccc cccatcaggg
     2341 tagattcagc gccgaagcca atcaggttaa tcccgttggt agcatgaacg gcgccgctag
     2401 cgcctgcaaa ggcgagagcg gttgcgaagg gtaggctaaa aaggcgggta gaaatcattg
     2461 cggcgtgaac tattattaat tgggtgtagt ttcgcatact ctggcaacgc tgtgtcgtca
     2521 taatagagcc ggaattacat cgtatttatg cttaagaaag ttagccggag ccatttgtac
     2581 acggatgagt gcaacagtct ttattctgat ttaaaggcat gactttcatc cgtgtgcata
     2641 gcaaatacgt gtgtattttc gcacgccaaa tcacaggtaa tcaatggtga gatcgcctgt
     2701 gtcacgggcg cggaaatatt ccccaataaa aacaatcagt tgctggttgt caatcaaaat
     2761 aaatcaatat attgtgtttt tggtaattga aaagccatag atattgtttt atagtcgcat
     2821 ccatacggta tgcagaagag cgtcctcttc tgcgtttaat agggaacctg gtgcgtgaaa
     2881 ctgcgcagga atcactaagc cagggctgcc cccgcaactg tgatcagcga gttgtttgct
     2941 gcaatcgacg ccactgagta aattcgggaa ggcacaagca aataatgatg acctgagagc
     3001 caggagacct accgtgattt ttttcttgtt aacgtttgag gcggggtgtc ctcggcagat
     3061 acgatttttt cagaagcggg cttgataaag gggttgctcg gcctgaaaaa atgatgtcta
     3121 tgccacttgt cgggtcacct tcgctgccgg aattgggagc caataatgtc acaatacgaa
     3181 tccatgcagg ccacgaaacg cgatggccgc aaggaaccta ttaatctcga caaaatccac
     3241 cgcgtgattg actgggccgc acaaggcctc aataacgtct cggtgtcgca agtcgagctg
     3301 cgctcccaca tccagtttta tgacggtatc cgcactgatg atatccacga gaccatcatc
     3361 aagtctgcgg ctgacctgat ctcggaagaa acaccagact accagtacct ggccgcacgc
     3421 ctgtcgatct ttcacctgcg caagatcgcc tatggccagt ttgagccgcc ccacctgttt
     3481 gaccacgtca cgaacctggt agagctgggc aaatacgata aacatttgct gagcgattac
     3541 agtcgcgaag aattcgatgt gctgaatggc tatatcgacc actggcgtga cctgaatttc
     3601 tcctatgctg cggtcaagca gttagagggc aaatacctag tacaaaaccg cgtcaccaag
     3661 aaaatctacg agagcccgca gttcctgtac atcctggtgg cgatgagcct gtttgccaat
     3721 tacaaagggc agaccaggct ggatctgatc aagcgtttct atgatgcagt gtccaccttc
     3781 aagatctcgc tgcctacgcc tattatggcc ggtgtgcgta cgccgacgcg ccagttcagc
     3841 tcttgcgtgc tgattgaatg tgacgacagc ctggatagca tcaacgccac ctctagcgcg
     3901 attgtgaaat acgtgtcgca acgcgccggt atcggcgtca acgcgggccg catccgtgca
     3961 ctgggcagtg aaatccgggg tggcgaagca caacacactg gctgcctgcc gttctacaaa
     4021 ttattccagt ctgccgtgaa atcgtgttca cagggtggtg tgcgtggtgg ggcggcgacc
     4081 ttgttctatc ctttgtggca cctggaagtc gagtccttac tcgtcctgaa aaacaaccgc
     4141 ggtgtagaag aaaaccgtgt gcgccacctc gattacggcg tgcagatcaa ccgcctgatg
     4201 taccagcgcc tgatcaaggg ccagaacatc accttgttct caccgcatga tgtgcctggc
     4261 atgtacgacg ctttctttgc cgaccaggac gagttcgagc gtttgtacac gcaatacgaa
     4321 gctgatgaaa gcatccgcaa acgcaccatg ccagcggtag atctgttctc actcatgatg
     4381 caagagcgcg ccggtactgg ccgtatctac atccagaacg tggaccactg caatacgcac
     4441 agcccatttg acccagcggt ggcaccggtt cgccagtcca acctgtgtat ggaaatcgcg
     4501 ctgccaaccc atccattgaa cgacatcaat gatgaagaag gcgaaatcgc gctgtgtacc
     4561 ttgtctgcgt ttaacctggg tgcgctggag tccctggacg agctggaagg tctggccgac
     4621 ctggtcgtgc gtgcgctgga tgccttgctg gagtaccagg attacccagt caaagcagca
     4681 ttgaacgcca ccaataagcg tcgttcactg ggcgtgggtg tcatcaacta cgcttactac
     4741 ctggcgaaaa atggcacggg ttacactgag gatgccgcgc tgggcctgac ccaccgcaca
     4801 tttgaggcga ttcagtatta cctgctcaaa gcctcggtac aactggcgcg cgaatttggc
     4861 ccatgtggtg cgtttaacga aaccacctac gccaaaggca ttttgccgat tgatacctat
     4921 aaaaaagacc tggatgccgt atgtagtgag ccattactgc tcgactggga aagcctgcgc
     4981 accgaaatcc agaaagacgg cttgcgtaac tcaaccttga cggcactgat gccatccgag
     5041 acgtccagcc agattgctaa cgccacaaac ggtattgaac cgccgcgcgg ccttgtctcc
     5101 gtcaagcagt ccaaagacgg tattttgcgc caggtggtgc cagagattga gcgcctgcgt
     5161 gaccaatacc agttgctgtg gaaaatgcct aacaacgacg gttatcttaa gctggtaggt
     5221 gtgatgcaga agtttgtcga ccaatcgatt tctgccaaca ccaactacga cccaacccgt
     5281 tttgaaggtg gccgtgtgcc gatgaaacag ctgctgaaag acttgctgca agtgtacaag
     5341 ttgggcatta aaacgctgta ctaccacaac accagagacg gagcgactga aggtggcacc
     5401 gaggcagaag atgatggatg tgccagcggt gcttgtaaaa tctaagagtt acaacgcata
     5461 cgcaatgcgc gtatgcgttt ggggttgatt taacaaaaaa tagcaataag tgagcgagcg
     5521 ggatgaagta caaggcaccc ggaacgcacg aaccgagaca gtacaaatag tacggcgagg
     5581 ttggtagtac cgcgtaacgc agtaattcgc ccgcgcagcc acttatctcc aaaggaacac
     5641 aagatggcct acagcatctt caccaaaacc gacaacgatc agttaaaaga acccatgttc
     5701 ttcggccagc ccgtcaatgt ggcgaggtac gaccagcaga aatatccggt attcgaaaag
     5761 ctgatcgaga agcagctttc tttcttctgg cgtccggaag aggtcgatgt gtcgcaagac
     5821 cgtgccgatt accaaggtct gcctgagcat gaaaagcata ttttcctgag taacttaaag
     5881 taccagacac tgctggattc cattcagggc cgtagcccta acgtggcctt gctgccattg
     5941 gtgtcactgc cagagctgga aacctggatc gagacctggg cgttttccga gaccatccac
     6001 tcgcgctcct acacccacat catccgcaac atcgtcaatg atccttccat catttttgat
     6061 gacatcgtgc gtaacgacaa catcaccgcg cgagcgtccg atattgcgca ttattacgat
     6121 gagctgattc agcagaccat gctgtattcg cagctgggcg agggcaatca tgtcatcaat
     6181 ggcgaaacca aagtagtgaa tcgccgtgag ctgaaaaagc tgctctaccg ctgcctgatg
     6241 aacgtgaata tccttgaggc gatccgcttt tatgtcagct tcgcttgctc gttcgccttt
     6301 gccgagcgca aggtgatgga aggtaacgcc aaaatcatcc gcctgattgc gcgtgacgaa
     6361 gccttgcacc ttaccggcac ccagcacatc ctcaatatca tgcgctccgg ccaggatgat
     6421 cccgagttcg ccgagatcgc cgccgagttg cacaacgaat gtttcgagat gtttaaaact
     6481 gctgccgagc aagaaaaaca atgggcagaa tatctgttta aagatggctc catgattggc
     6541 cttaataaag acatcctgag ccaatacgtg gaatacatca ctaacagccg catggcctcg
     6601 gtcggtttgc cacccgcgtt ccctaacgcc aaaaataacc cgattccatg gatcaacacc
     6661 tggctggcct cagacactgt gcaggttgca ccgcaagaag tagaattaag ttcatacctg
     6721 attggtcaga ttgattccga ggtttcggct gacgatttaa acgactttga actgtaataa
     6781 gtggctttgc gggcaaattg ctacgttgtg cggtttttta gtttcggcat aacataaatg
     6841 ttagcctaca ctgaaactcc gttttcacaa cttcgccgta ctatctgtac tgtctcagtt
     6901 gttgcgttcc gtgcgccttg cactttatct cgcacagtca cttattgatt gtttcgctat
     6961 taagcttgaa aagacttttg tacag
//
LOCUS       DATMSK010000181         4083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_319822,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000181 DATMSK010000000
VERSION     DATMSK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319822"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..336
                     /locus_tag="VNZ84_05945"
     CDS             109..336
                     /locus_tag="VNZ84_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWU66534.1"
                     /translation="MYVCVCNAVTERQIHLAVKNGARTVKHLKDELGVGAECGKCASC
                     AKACLKEAHAEQHPGLVKKLIQLTPVQLGAA"
     gene            451..1359
                     /locus_tag="VNZ84_05950"
     CDS             451..1359
                     /locus_tag="VNZ84_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWU66535.1"
                     /translation="MTETSSPALHWRTLVLLLLPPLLWAGNFIAGRAINTEVPPVMLS
                     LIRWSIAAVCLLPFAISPFLKDWRSYRQYRWHVLGTAITGVATFNLLVYWGLHTTSAT
                     NGIILNSFIPFLVAIFGFLLYRHQLPGNQLVGLLVSLVGVLTVVSHGDWQILSSLAFA
                     PGDLIIFLAMIFWALYTLWIKEIPANIHRTGLMLCQVFIALLLLAPLAALESMTTSTH
                     LQNANTLWILGYIGIFPSVIALLAYSKAVTIVGSVRASVFIHAMPIFGSILSTLLLHE
                     TFHLYQLLGMLTIFTGIWLANRTSKT"
     gene            1439..1945
                     /locus_tag="VNZ84_05955"
     CDS             1439..1945
                     /locus_tag="VNZ84_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWU66536.1"
                     /translation="MAIVFNPELDLILEREVDVTPAQIWHAWTTPEHLMKWFCPLPWK
                     TVACEIDLKPGGLFRTVMQSPEGQQFPNIGCYLEVIPNKKLSWTNALQPDFRPAKTPE
                     LSPGHECAEFLMTATLLLEATTSGTRYTAYVLHADSASKQRHEAMGFEQGWGIVLDQL
                     VAEIKKTR"
     gene            complement(1956..2669)
                     /locus_tag="VNZ84_05960"
     CDS             complement(1956..2669)
                     /locus_tag="VNZ84_05960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HWU66537.1"
                     /translation="MIARQLQLPDGQHITYQLDLRARKTIGLKITANGLVVHAPKRIS
                     ATQLQQVLLEKSGWIQQKLALREANQVAPMQWQDGELLLFMGQDIVLALQHHGTNKAV
                     ILQGNQLLVRSPQIDQPAFVARKVLQWYAQQALPDFTRRVELIAAQMGEKISGVALSN
                     AKSRWGSCNSRRQIRLNWRLIQAPPHIIQYVVCHEMAHLREMNHSARFYAVQESLFPN
                     HVQAEKELRALSPILHRMA"
     gene            complement(2666..3379)
                     /locus_tag="VNZ84_05965"
     CDS             complement(2666..3379)
                     /locus_tag="VNZ84_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWU66538.1"
                     /translation="MQKAVLYVRAWLFYVGLFLLTIPFSLLAILLIPLPAVMRSRWVS
                     GWAHAVMWWLKVTCNLRYEVTGREHIPATPSIILAKHQSAWETIALQSIFPPQVWVLK
                     RELLMIPFFGWGLWAVGSIPIDRSSGREALKKLVNHGKDRLKRGLWVVIFPEGTRTAP
                     GARGKYHIGGAWLASHTETAVVPVAHNAGRFWRKNSILKYPGVIQVVIGQPIDTTGLK
                     PDAVNKQVEEWIEAEMLQL"
     gene            complement(3379..3921)
                     /gene="gmhB"
                     /locus_tag="VNZ84_05970"
     CDS             complement(3379..3921)
                     /gene="gmhB"
                     /locus_tag="VNZ84_05970"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HWU66539.1"
                     /translation="MKLVILDRDGVINRDSATFIKNPNEWIPIPGSLEAIAQLNQHGY
                     RVAVATNQSGIARGYFDMATLNAIHDKMHKALAQVGGRIDAVFYCPHAADDHCNCRKP
                     NTGMIEEIGRRFNLELAKVPGVGDALRDLQAFDKAGCQPYLVRTGKGEETYNLADVPM
                     NTIVVADLAAAVQDIIAGDK"
BASE COUNT         1017 a         1088 c          950 g         1028 t
ORIGIN      
        1 ttaagccgcg aaagcggctt ttttcttggc cgttaggaac caatttcgcc ttgacaaact
       61 cttattaaat gataataatt atcattagtt attttaatac gaggcaatat gtacgtttgc
      121 gtgtgtaatg cagtcactga gcgccaaatt cacttggcag tgaaaaatgg tgcaagaacc
      181 gttaagcatt taaaagatga attaggcgta ggcgctgaat gcggcaaatg tgccagctgc
      241 gccaaggctt gccttaaaga agcgcacgct gaacaacatc ctggcttggt caaaaaactg
      301 attcaactga cacccgtaca attaggcgca gcttaatcta aaaccgattc tgaaaagggg
      361 cctctggccc cttttgttat tctaagccag actctttgca cctcagccaa tcgccgtcta
      421 caatctggat tccccatttt gaccatccgc atgactgaaa cttcttcacc agcgctacac
      481 tggcgcaccc tggttttatt attactgcca ccactcttat gggctggtaa cttcattgcc
      541 gggcgcgcca tcaatacaga agtgccgccc gtcatgcttt ccctgattcg ctggagcatt
      601 gctgcagttt gtctgctacc gtttgccatt tccccttttt taaaagactg gcgaagctac
      661 aggcaatacc gctggcatgt gctgggcact gccataaccg gcgtcgcgac atttaactta
      721 ctcgtgtact ggggcttaca tacgaccagc gccaccaatg gcatcatcct caattcattt
      781 attccattcc tggtagccat ttttggtttt ctgctctacc ggcatcaact gcctggcaat
      841 caactggtcg ggttgctcgt atcgctggtt ggcgtactca ccgtcgtctc ccatggtgac
      901 tggcagatat taagttcttt agccttcgct cccggcgacc tgattatttt ccttgccatg
      961 attttctggg cactgtatac cttatggatc aaggaaattc cggctaacat tcatcgcact
     1021 ggccttatgt tatgccaggt atttattgct ttgttgctgt tggcgccatt ggccgcgttg
     1081 gaaagcatga caacaagtac ccatctgcag aatgcaaaca ccttatggat attaggctac
     1141 atcgggattt tcccgtcagt gattgcacta ctggcttatt ccaaagcggt aacaatagtt
     1201 ggctcagtgc gcgccagtgt ttttatacac gccatgccga ttttcgggtc gatactctca
     1261 accttgctac tacatgaaac attccatctt taccaactac tgggtatgct gaccattttt
     1321 actggcatct ggctggcgaa ccgcacaagc aagacgtaag catttaaact ttactaaccg
     1381 ctgtcagcta gctttttatt ggcggttatt catttcaaaa cgtcaaggag atcaagtgat
     1441 ggcaattgta ttcaaccctg agctggattt aattctggaa agagaagtag atgtaacgcc
     1501 ggcgcaaatc tggcatgcgt ggacgacccc ggaacacctg atgaaatggt tttgcccgtt
     1561 accttggaaa acagtggcat gtgaaattga cttaaaaccc ggcggtcttt tccgcacggt
     1621 gatgcagtcg cccgaagggc agcaatttcc caatattggc tgttatctcg aggtgatccc
     1681 aaacaaaaaa ctgagttgga ccaatgccct gcaaccagac tttaggccag caaagacgcc
     1741 ggaactttca cctggccatg aatgtgcaga attcttgatg actgccaccc tgctactgga
     1801 agccaccaca agtggaacca gatataccgc ctatgtattg catgcggatt ccgcatccaa
     1861 acaacggcat gaagccatgg ggtttgagca gggctgggga atcgtgctgg atcagctggt
     1921 ggccgagatc aaaaaaacac gctaagaaga agcgatcagg ccatccggtg caaaatcggc
     1981 gacaatgccc gaagttcttt ttcagcttgc acatggttcg gaaacaaact ttcctggacg
     2041 gcataaaacc tggcggaatg gttcatttcc cgcagatgcg ccatttcatg acagacgaca
     2101 tactggataa tgtgtggcgg tgcctgtatc aagcgccagt tcaagcgaat ctgcctgcgg
     2161 ctattacaac tgccccaacg tgatttggca ttggaaagcg cgacaccact tattttttcg
     2221 cccatttgtg cagcaattaa ctctacccgg cgagtgaagt ctggcagcgc ctgctgtgca
     2281 taccattgca gtactttgcg tgcgacaaag gcaggctggt cgatctgcgg cgacctgacc
     2341 aaaagctggt tgccttgtag tatcactgct ttattagtgc catggtgctg cagtgccaac
     2401 acaatatctt gccccataaa cagcagaagt tcaccatcct gccactgcat gggtgccacc
     2461 tggttggctt cacgcaaagc cagtttttgc tgtatccaac cggacttttc cagtaaaact
     2521 tgctgcagct gtgtggctga aatacgcttg ggcgcatgca ccaccaagcc attggccgta
     2581 attttaagac cgatggtttt acgtgctctc aggtcaagct ggtaggtgat gtgttgccca
     2641 tctggcaatt gcaactgccg tgcaatcaca gttgcaacat ctccgcctca atccactctt
     2701 caacctgttt attgacagca tccggcttga ggccagtcgt atcaatcggc tgaccaatca
     2761 ccacctggat cacgccagga tatttaagaa ttgaattttt acgccagaaa cgccctgcat
     2821 tatgcgcgac cggcacaacc gcagtttcag tatgtgatgc cagccaggca ccaccgatat
     2881 gatatttgcc acgtgcaccc ggcgccgtac gggtgccttc tggaaaaatc actacccaca
     2941 agccacgctt gaggcggtcc ttgccgtgat tcaccagttt tttcaaggcc tctcggccgc
     3001 tactgcggtc aatcgggatg gaccccaccg cccataaacc ccagccaaaa aacgggatca
     3061 tcagtaactc acgtttaagc acccaaactt gaggcgggaa aatactttgc agtgcgatag
     3121 tttcccaggc tgactggtgc ttggccagga taatagaggg cgttgccgga atatgctcac
     3181 ggcctgtcac ctcatagcgc aggttgcaag tgactttaag ccaccacatc actgcatgtg
     3241 cccagccact cacccagcgc gagcgcatca ctgctggcaa cgggatcagc aaaatagcca
     3301 gcaacgagaa tggaatggtc aatagaaaca ggccaacata aaacagccag gcacgcacgt
     3361 acaacacggc cttttgcatt atttgtcacc tgcaatgatg tcttgaacgg cagcggccag
     3421 atcagcaacc acgatggtat tcatagggac atccgccaaa ttataggttt cttcaccttt
     3481 acccgtacgc accaggtaag gctggcatcc tgccttgtcg aaggcttgca aatcacgcag
     3541 ggcatcacca acgcctggca cttttgccag ctcaagatta aaccggcggc caatctcttc
     3601 aatcatgccg gtgtttggtt tacggcagtt acaatggtca tcagctgcat gcgggcaata
     3661 aaacaccgca tcaatgcggc cacccacttg cgccaaggct ttgtgcattt tgtcgtggat
     3721 ggcattcagc gtcgccatgt cgaaatagcc acgcgcaatg ccagattgat tagtcgctac
     3781 cgccacgcgg taaccatgct gatttaattg ggcaatcgcc tccaggctac ctggaatcgg
     3841 aatccactca ttaggatttt tgataaaggt tgcactgtcg cggttaatca cgccgtcacg
     3901 gtcgaggatg actaatttca tacataaact cctgacggca atagattaac tggccagctt
     3961 ggacaaatca gcgacacgat tcatcgcctg atgcaaggta gccagcaagc ccaaacggtt
     4021 ggctttgagt gccggatcct ctgcattcac catgacctcg tcaaagaagg tgtctaccgg
     4081 cgc
//
LOCUS       DATMSK010000182         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_13958,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000182 DATMSK010000000
VERSION     DATMSK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13958"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /gene="clpS"
                     /locus_tag="VNZ84_05975"
     CDS             complement(<1..260)
                     /gene="clpS"
                     /locus_tag="VNZ84_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWU66540.1"
                     /translation="MVRPKREGDLALQSQVAKPKPPELFKVLLLNDDYTPMEFVVAVI
                     ERFFNKSREQATQIMLKVHTEGMGVCGVYPQDIAETKMHQVL"
     gene            complement(341..1612)
                     /locus_tag="VNZ84_05980"
     CDS             complement(341..1612)
                     /locus_tag="VNZ84_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HWU66541.1"
                     /translation="MDNIPLSWQIGLLLLLLSLTAFFSIAETSLMSLNRYRMRHLAKE
                     GHRGAKLASLLIAQTDKTLGAILLGNTFCIVGSSTLEVIISKRLFGEGDYVLMVGSLA
                     ITFAILVFSEISPKVIAAAHAEKLGFASSYLLYPLLWLFNPVVWFVNLFVKAFLKLLG
                     VRINFNGEAQAVTSEELRSIVSDAGQYIPSQSRTILLNLLDLEDIIVDDAMTAHTMVE
                     FIDLDHPIAQIMEQLARSHHSRLPVRQGDHEDILGILHVRKVLPLLRDFHTGKAPTKA
                     LLQAAMSLPYYIPSGTPLYTQLQQFQKRHERIALVVDEYGEFKGLLTLEDILEEVVGE
                     FTTQSPDHVSHFHQNEDGSWLVDGSSLLRDLNRKLKLSLPTDGPRTLNGLILEHFEDI
                     PEPDTSFKIGEHRLEILDAQDRIVKRVKIYL"
     gene            complement(1697..2641)
                     /gene="secF"
                     /locus_tag="VNZ84_05985"
     CDS             complement(1697..2641)
                     /gene="secF"
                     /locus_tag="VNZ84_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWU66542.1"
                     /translation="MELFRIKKDIPFMSYGRLTTSISLITFILAIFFLLHRGLNYGVD
                     FTGGTVMEVHYPAAANIEQIRQVMDAVGLKDVTVQNFGSSQDVLIRLPVKKELSIAQL
                     SEKISTALKGADAGAEVRRVESVGSQVGEELYENGALALLLVCAGIMGYLALRFEWRF
                     AVAAIIANMHDVIIILGFFAFFQWEFNLTVLAAILAVLGYSVNESVVVFDRVRENFKK
                     MRKATSVQVIDNAITRTMSRTIITHLMTQTMVCSMLFFGGETLHNFSLALTIGILFGI
                     YSSVLVACPIAMWLGVSRANLIGDAEKKPKEGETAVAP"
     gene            complement(2702..>4271)
                     /gene="secD"
                     /locus_tag="VNZ84_05990"
     CDS             complement(2702..>4271)
                     /gene="secD"
                     /locus_tag="VNZ84_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWU66543.1"
                     /translation="KDKLVANLGKDYTVALNLVPQTPGWLQSIGAKPMYLGLDLRGGV
                     HFLLQVDMKAALDKAAESTVGDFRMSLRKEKSNYYGIERQGQVVVVKFESEAEATKAR
                     NLLAKDFSDLTYKISGSDKDFSLTASMSVMAQKRIQEFALKQNLQTLHNRINELGVAE
                     PIVQQQGLDRIVVQLPGVQDTAKAKEILGRTATLEIRMVDEEKSDMATLQKAQAGQVP
                     FGDELFSDREGRPVLVKKSVVLTGDRITDAGPGVNGQNGASVVNVTLDGRGAGIFKNV
                     TRENVGKRMAILLIEKGSTEVITAPVINEEIGGGRVQISGMANAQEATDISLLLRAGA
                     LAAPMEIIEERTVGPSMGEENIKRGVHSTLWGFAAIAVMMIVYYMVFGGVSVMALGVN
                     LLLLVAILSMLQATLTLPGLAAIALALGMAIDANVLINERIREELRNGNTPQASIHAG
                     YDRAFDTILDSNVTTLIAGLALFMFGTGPIKGFAVVHVLGIMTSMFSAVLVSRALVNL
                     LYGYRRKIEHLRIG"
BASE COUNT         1062 a         1115 c         1073 g         1021 t
ORIGIN      
        1 tctaatacct gatgcatctt ggtttccgca atatcctgcg ggtatacacc gcatacgccc
       61 atgccctctg tatgtacttt gagcatgatt tgtgtggctt gttcgcgaga tttgttaaaa
      121 aagcgctcaa tgaccgcgac aacaaactcc atgggggtgt agtcgtcgtt gagcaacaac
      181 accttgaata gctcgggagg cttgggtttg gccacttggg actgtagagc caaatcacct
      241 tcacgcttgg gtcgtaccat agttttgtat acctgtctta ggacttattt tgcgccttgt
      301 attaaaaaat tcaagagctg gatgcggaag aaatccgctt ttaaagataa atcttgacgc
      361 gcttgacgat acgatcctga gcatcgagga tttccagccg gtgttcacca attttaaagc
      421 tggtatccgg ttccggaatg tcttcaaaat gttcaagaat caatccattc aatgtgcgtg
      481 gaccatcggt gggcagggag agtttcaatt tgcggttcag atcacgtaac aggctgctgc
      541 catccactag ccagctgccg tcttcattct ggtggaaatg gctgacatga tctggtgatt
      601 gggtcgtgaa ttcgccgacg acttcttcca gaatgtcctc cagggtcagc aagcccttga
      661 attcaccgta ctcatcgacg acgagggcaa tacgctcatg ccgtttttgg aattgttgta
      721 attgcgtata gagcggggtg ccagaaggaa tgtaataagg caaactcatg gccgcctgca
      781 acaaggcctt ggtgggcgct ttgcctgtgt ggaaatcacg cagcaggggc aagactttac
      841 gcacatgcaa aatgcctaag atatcttcat ggtcgccctg gcgcacaggc aggcgcgaat
      901 gatggctgcg agccagttgc tccatgatct gggcaatcgg gtgatcaaga tcgataaact
      961 ctaccatggt atgtgcggtc atggcatcgt ccacaataat atcttccaga tcgagcaggt
     1021 tcagcaggat ggtgcggctt tgcgagggaa tatactgccc cgcatcgctg acgatgctgc
     1081 gtaattcttc ggaggtcact gcttgcgcct cgccattaaa gtttatacgt acccccagca
     1141 atttgaggaa ggctttgaca aataaattga caaaccagac cacgggatta aacagccata
     1201 acaatgggta tagcaggtag ctactggcaa agccaagctt ttcggcatgc gcggccgcaa
     1261 tgaccttggg agagatttcg ctaaacacca ggatggcaaa ggtaatcgcc aaggagccga
     1321 ccatcagcac ataatcgcct tcaccaaaca ggcgcttgga aataatcacc tctagtgttg
     1381 acgagccgac gatgcaaaag gtgttgccaa gcaggatggc gcccagggtt ttgtcggttt
     1441 gggcaatcag caggcttgcc agctttgcgc cacggtggcc ttctttggcc aaatgccgca
     1501 tgcggtagcg gttgagcgac attaaactgg tttcggcaat ggagaaaaaa gccgtaagag
     1561 acagtaatag cagtagcaga ccaatctgcc agcttaaggg gatattgtcc aaaagtgtgg
     1621 ggttaaaaac aagaagccac tagtgtaacc accagtggct aaaaatccaa gcctaaaaat
     1681 caagcagcta tacagcttaa ggtgcgacgg cagtttcacc ttctttgggt ttcttctctg
     1741 catcgccaat caggttagcg cgactgacac ccagccacat ggcaatcggg caggccacca
     1801 acacagagga gtaaatgcca aacaaaatac caatggtcag tgccagcgag aagttgtgca
     1861 gagtttcgcc gccaaaaaac agcatggaac agaccatggt ttgtgtcatc aagtgcgtga
     1921 tgatggtacg tgacatggtg cgtgtaatcg cattgtcgat cacttgcact gaagtggctt
     1981 tacgcatttt tttgaagttt tcacgtaccc ggtcaaacac cacgacggat tcgttaaccg
     2041 aatagcctaa gactgccagg atcgccgcca ggaccgtgag gttaaattcc cactggaaga
     2101 aagcaaagaa accgagaatg atgatgacgt catgcatgtt ggcgataatc gctgccacag
     2161 caaaacgcca ttcaaaacgc agcgccaggt agcccataat gcccgcacat accagcaata
     2221 aagccagggc gccattttca tacagttctt cacctacttg tgagcctact gactccacgc
     2281 ggcggacttc tgcgccggca tccgcgcctt ttagcgcagt agagattttt tcagatagtt
     2341 gtgcaatgga taattctttt tttaccggca agcggatgag cacgtcctgg ctggagccga
     2401 agttttgtac cgtcacatcc tttaagccca cggcatccat cacctggcgg atttgctcga
     2461 tattggcagc cgctgggtag tgcacttcca tgaccgtacc accagtgaaa tctacgccgt
     2521 aattcaggcc gcgatgtagc aggaagaaaa ttgccaatat aaaggtaatc agcgaaatcg
     2581 aggtggtgag acgcccgtag ctcatgaacg ggatgtcttt tttaattctg aataattcca
     2641 taatgtattc tcttttatgc gttgtcagcc attgcgctat gcgcgattaa cagatgcgcg
     2701 attaaccaat ccgcaggtgc tctatcttgc gacggtagcc gtacaagagg ttgaccagcg
     2761 cacgtgatac cagcacagca ctaaacatgg aagtcatgat gcccagcacg tggacgacag
     2821 caaagccctt gatcgggccg gtaccaaaca taaacaacgc caggcccgca atcagggtcg
     2881 tgacgttgga gtccagaatg gtgtcaaatg cacggtcata accggcatga atgctggctt
     2941 gaggcgtgtt gccgttgcgc aattcttcac ggatacgttc gttgatcagt acgttggcat
     3001 caatcgccat ccccaatgcc agcgcaatcg cagccaggcc tggcaaggtg agtgtcgctt
     3061 gcaacatgga caagatcgct accagcaaca gcaggttgac gcccaaggcc atcacagaca
     3121 caccaccaaa gaccatgtag tacacaatca tcatgaccgc aatcgctgca aatccccaca
     3181 gggtagagtg cacgccacgc ttgatgtttt cttcacccat gctaggacca acggtacgtt
     3241 cctcaatgat ttccatcggg gccgccagtg cgccagcacg caacagcaat gaaatgtctg
     3301 ttgcttcctg cgcattcgcc atgccggaaa tttgtacgcg gccaccaccg atttcttcat
     3361 tgatgaccgg cgcggtaatg acttcagtac tacctttctc aatcagcaga attgccatgc
     3421 gcttgccgac gttttcacgg gtcacgtttt taaagatgcc ggcaccgcgg ccatccagcg
     3481 tcacattaac cacagacgcg ccattctggc cattcacacc agggcccgcg tcagtgatac
     3541 ggtcaccggt cagcacgacg cttttcttca ccaatacagg gcggccttca cggtcactaa
     3601 acaactcatc accaaagggt acctggcccg cttgcgcttt ttgcagcgtc gccatgtcag
     3661 atttctcttc atccaccatg cggatttcca gcgtggcggt acggcccaga atttccttgg
     3721 ctttggcggt gtcctgtacg ccaggcaatt gcaccacgat gcggtccagg ccttgttgct
     3781 gcacgatagg ttcagccacg cccagttcgt tgatccggtt gtgcaaggtt tgcaggtttt
     3841 gcttgagcgc aaactcctgg atgcgttttt gcgccatcac gctcatgctg gccgtcaggc
     3901 taaagtcttt atcactgccg gaaatcttgt aagtcaggtc agaaaagtct ttggccaaca
     3961 gattgcgtgc tttggtggct tcggcttctg attcaaactt caccaccaca acctggcctt
     4021 ggcgctcaat gccgtagtag ttgctttttt ccttgcgcag cgacatgcgg aaatcgccga
     4081 ctgtactttc agctgccttg tccagcgccg ctttcatatc cacttgcagc aggaaatgca
     4141 caccaccgcg caagtccagg cccaaataca taggtttggc accgatgctt tgcaaccagc
     4201 ctggtgtctg tggcaccagg ttgagggcga cggtatagtc tttacccagg ttagcaacca
     4261 gcttatcttt c
//
LOCUS       DATMSK010000183         9118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_178126,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000183 DATMSK010000000
VERSION     DATMSK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178126"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..872
                     /locus_tag="VNZ84_05995"
     CDS             75..872
                     /locus_tag="VNZ84_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWU66544.1"
                     /translation="MASVINTNLASLNAQRNLTANQSALNTSIQRLSSGLRINSAKDD
                     AAGMAIATRMDSQVRGMDVATRNANDAISFLQTAEGGLSSATDALQRMRELAVQAANG
                     SYSSGDRANLDTEFSQLKSELTRLSTATKFNGQAAFGTGYNFQIGADSGDTISVASVA
                     AASLSGSLSSADAASAAITAIDAQLTTVNTNRATLGAYQNRFASVVSNLGVASENMAA
                     AKSRITDADFASETAKMTRAQILQQAGTAMLAQANQLPNNVMTLLRG"
     gene            945..1289
                     /locus_tag="VNZ84_06000"
     CDS             945..1289
                     /locus_tag="VNZ84_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HWU66545.1"
                     /translation="MSDSSVSGVNGGLQGVQPASSLKLVKYAESAAEKAAADVNSKAA
                     LTGAVKKLNEYVAPALQTIEFSIDDDTDRIIVKVVDTETQQVLRQIPNEEVLAISKTL
                     DKLRGLVVRQTA"
     gene            1323..3002
                     /gene="fliD"
                     /locus_tag="VNZ84_06005"
     CDS             1323..3002
                     /gene="fliD"
                     /locus_tag="VNZ84_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HWU66546.1"
                     /translation="MASIVSSTGASGLPIDSLVTAQMQLEQQPLTDIQTKISSYNTKL
                     SAYSTLKSGLSTFQTAVTKLATAAQFNAQSVTASDATSISATANGTAVVGNYSVSVSQ
                     LATAQKLATSAFSSATDTVGTGKITISFGTYTAAAGSDPASFTANSDKSDITIDITSS
                     NNTLAGIRDAINAKNASVSASIVNDGSGNRLVITSKDTGEVNSLKISVADDDGNNTDT
                     GGLSALAYDPLASTNSMTQMVAAKNALLTVDGMSISKSSNTVSDVIQGVTLTLKAVTS
                     TSNTLSVGTDSDTIQSSVQSFVDAYNALNTSMRNLTKFVSAGSSSNGVLLGDSTARNI
                     MVKLKSMLSASSPTATTYKTLSDIGVSMGSDGSLSLDSTKLQKAITNNVSDVAKLFSP
                     SATSTDPQVTFVSSKDDTVSGTYAVNITRLGGNGVDAAGTINGVTAITTGSILTGAGG
                     DASYGLQLSVTGTATGSRGTVTFSKGLAGELNSLLTSWLDTDGALATKTDGINSSIKD
                     LNKKADDINAKLPSIEARYRAQYAKLDALLSSLQSTSSSLTSQLAAISANG"
     gene            3084..3587
                     /gene="fliS"
                     /locus_tag="VNZ84_06010"
     CDS             3084..3587
                     /gene="fliS"
                     /locus_tag="VNZ84_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HWU66547.1"
                     /translation="MFGMKKSGVNAYASVGLETGDVDASPLKLIVMLYDGAITACIKA
                     QQAMAQNDIAKKGEYLTQAVTIVDSGLRTCLDKRAGGLIAQNLDQLYQYMTRSLMEAS
                     VRQDVKKVQEIQQLLMELKGAWETLEKSAVTRPAQVDMSLGQMVAQKTHELQHMGQAN
                     RLATAGA"
     gene            3589..3921
                     /locus_tag="VNZ84_06015"
     CDS             3589..3921
                     /locus_tag="VNZ84_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HWU66548.1"
                     /translation="MSQALINTYESAAKLMEQMLIAARQNAWEKVTELEQSYMLKIEV
                     VKTLEQNMRLASEKLSPDLHARKQVLVQKIMADDGEIRNLLYPVMHRITDMIFDAQLH
                     ARIPPDVT"
     gene            3922..5055
                     /locus_tag="VNZ84_06020"
     CDS             3922..5055
                     /locus_tag="VNZ84_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HWU66549.1"
                     /translation="MLPIPQVLTQRFIASETESLRAVSSILPVEGTQAEAPLWQRMRP
                     GMQFTGLIVQKEAAGENGSELAQFKVQIQLPNQPPALVQMQLPAYLAQQQTLNMQFMG
                     LAQQSTGKDALPQVKWGLQQGNANAGSARLGDAALLTDMSQPEGSEHLQDMVKTAAGQ
                     ASLVELSNPAKHLQRWLSSSEFQQQTNALQAQAVVSHSPQKPQVLAQDLKHAMDNSGL
                     FYESHLKEATVGSRPWHQLLQEPQNKPSFAPPEMVAQQLQVLEQQRVVWHGEVWPGQT
                     MQWQVNEQESSPQSSEEPVLNSLQSDLTLHLPRLGDVKVNIAMVNGRFSVHILPSESQ
                     ALKTMQGSRSQLAMSLQSAGLKLDALQITRDEADEDRRQYAAR"
     gene            5042..5332
                     /locus_tag="VNZ84_06025"
     CDS             5042..5332
                     /locus_tag="VNZ84_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HWU66550.1"
                     /translation="MQRDKAYTQSAVALAYEAGDLAPRVVAKGNGLLAERIIALAKEH
                     EVYVHESQALLSLLMQVELDAHIPPQLYQAIAEILAWLYQLEQGELREPPAV"
     gene            complement(5395..5760)
                     /gene="fliE"
                     /locus_tag="VNZ84_06030"
     CDS             complement(5395..5760)
                     /gene="fliE"
                     /locus_tag="VNZ84_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HWU66551.1"
                     /translation="MNAGGIDAQRITSMLAQLRAAAQKPEGLGSSAIASPVQKTNETS
                     PTSQVSFSDALKASLDQVNNMQLQSEKLGQDFAMGNDNVSLSDVMISGQKANIAFQAT
                     VQVRNKLVTAYQDMMNMQV"
     gene            6024..7730
                     /gene="fliF"
                     /locus_tag="VNZ84_06035"
     CDS             6024..7730
                     /gene="fliF"
                     /locus_tag="VNZ84_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HWU66552.1"
                     /translation="MAADAALLTSNDESNGSPNMFGQLGQKLMLVAGIAAVVAVMVVF
                     WLWSQKPDYRVLFSNFADKDGGAIVAELDKLNVPYQFAEGGNAILVPADQVHQVRLKL
                     AAQGLPKGGNIGFELLENQKFGVSQFVEQVNFQRGLEGELERSIQSISAVQAARIHLA
                     IPKPSVFVREQQYPTASVLLNLHNGRRLDAQQVAAVVHLVASSVPNLSADHVTVVDQN
                     GNLLSDNANKIKQNGLDPEQMAYVENMQNSIARRVESIITPIVGAKNVHAETSAEIDF
                     SMTEQANESYKPNTKPDDMAIRSAQSHEAQNAAGSSGAVPGALSNQPPGDSTAPINAP
                     GAQAPGDNAAAAAPPPAPPQNTQKDTTTNYELDKTVSYIQQAKGGIKRLHVAVVVNHI
                     PVVDSTGKTTYRALSNAEKQQVSDLAMQAMGFNKERGDTISVVNTPFIAEAQEKLAEP
                     PLWKDPLVIEYGKDALRFIAGVIVLMMIYKKLLKPMANKLTGADKKQLALAGTPALAG
                     PDGAAVAAGADGEDALVTLSGDATALPSSLDQTLEAAKQVAKENPRMVANVVSSWTSA
                     EK"
     gene            7734..8732
                     /gene="fliG"
                     /locus_tag="VNZ84_06040"
     CDS             7734..8732
                     /gene="fliG"
                     /locus_tag="VNZ84_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HWU66553.1"
                     /translation="MSDQGLTRSAILMLALGEDEAAEVMKHLTPKEVQRLGIVMASMK
                     PVPREEVEQVLEKFMLDFASSSDFGLDSDSYIRSVLIKAFGDDKASNLLNRIPQSQDA
                     AGIESLKWMDAFSVADFIKNEHPQIIATILSHLEAEQSAEILGHFTERLRQDVILRIA
                     TLDSIKPTALRELNEGMLKMLAGNDNIKRQAIGGVKTAAEIMNFISGEHEAKLMDGLK
                     SYDDGMAQQIMDQMFVFDNIMEIDDRGIQVLLREVQSDMLIVALKGTSDEMKEKFFRN
                     MSSRAADMMREDLESRGPVKLSEVEGQQKQILQIVRRLADEGQIMLAGKGDSEQYV"
     gene            8757..>9118
                     /locus_tag="VNZ84_06045"
     CDS             8757..>9118
                     /locus_tag="VNZ84_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HWU66554.1"
                     /translation="MVASAIPKEQMSAYQRWEMASFDDSDSSERVKRERAQAEENART
                     VSQILKQVRQEAYEEGFKTGYVDGMGHAEKQLSAEREQLLQMAGNFQQALSMQDVSVA
                     ESVLSLALDLAKAMMKTK"
BASE COUNT         2431 a         2151 c         2432 g         2104 t
ORIGIN      
        1 aaatagcgat caacgttgaa agtaacatga cgatttgaca cttaactgga atacgactct
       61 aaaggagatt taacatggct tcagtaatta acaccaactt ggcatccctg aatgcacaac
      121 gtaacttgac cgctaaccag tcagctctga atacttctat tcaacgcttg tcttctggtc
      181 tgcgtattaa cagcgctaaa gatgacgcgg ctggtatggc gattgcaaca cgtatggact
      241 cacaagttcg cggtatggat gttgcaacac gtaacgcaaa cgacgcgatt tccttcctgc
      301 aaactgcaga aggtggtttg agcagcgcaa ctgatgcatt gcaacgtatg cgtgaactgg
      361 ctgtacaagc agctaacggc tcttacagct ccggtgaccg tgcaaacctg gatacagaat
      421 tctcacaact gaagtcagaa ctgactcgtt tgagcacagc aaccaagttt aacggccagg
      481 cagcattcgg tacaggttac aacttccaga tcggtgctga cagtggtgat accatcagtg
      541 tggcttctgt agctgctgcc tcattgagcg gttccctgtc ttcagccgat gctgcatcag
      601 ctgcgatcac tgcgattgat gcacaattga ctacagtgaa cactaaccgt gcgaccttgg
      661 gtgcttacca gaaccgtttc gcttcagtag tgagcaactt gggtgtggct tctgagaaca
      721 tggctgctgc caagtctcgt atcactgacg ctgacttcgc ttctgaaaca gccaagatga
      781 cacgtgcaca gattctgcaa caagctggta ctgcaatgtt ggcacaagcg aaccaattac
      841 ctaacaacgt gatgacatta ctgagaggtt aattcatcag acggtaatgc taaatccaca
      901 ggcctagccc ctgtggattt ggcctaggta aaaggagcaa catcatgagt gattcatctg
      961 tgagtggtgt aaacggtggt ttgcaaggtg tgcaaccagc cagtagtttg aaactggtga
     1021 aatatgctga atctgctgct gagaaagctg cagccgatgt gaatagcaaa gctgcgctaa
     1081 ccggtgcagt caagaagtta aatgagtatg tagccccagc gttacaaacg atagagtttt
     1141 ctatcgatga tgataccgac cgtatcattg tgaaagtggt ggataccgaa acacaacagg
     1201 tattacgcca gattcccaat gaagaggtgc tggccatcag taaaacgctg gataaattac
     1261 gtgggctggt ggtgcgacaa actgcctgat atcacgttga atagacggtt gggagattaa
     1321 aaatggcatc aattgtatcc tctacaggcg catcgggttt accgattgac tcgctggtca
     1381 ctgcgcaaat gcagttagag cagcaacctc tcaccgatat tcaaaccaag atatcttcat
     1441 ataacaccaa actatccgct tatagcacgc ttaaaagcgg attaagtact ttccagacgg
     1501 ccgtgactaa attggctact gcagcccaat tcaacgcaca atccgtgaca gccagtgacg
     1561 cgaccagcat cagcgccacc gcaaacggga cggctgtggt cggtaactac agtgtcagcg
     1621 tcagccagtt ggcgacggct caaaaattgg caacttcagc gtttagcagt gccaccgaca
     1681 ccgtgggtac cggcaaaatc actatttctt tcggcactta tacggctgct gccggcagtg
     1741 atccagcttc attcactgcg aatagtgaca agtcagatat caccatagac attaccagta
     1801 gcaacaatac actggctggc atccgcgatg cgattaatgc aaagaatgcc tctgtttccg
     1861 catccattgt gaatgatggc agtggtaacc ggctggttat tacctccaaa gataccgggg
     1921 aagtgaatag cctgaaaata tctgtggcag atgatgatgg taataatacg gacactggtg
     1981 ggctgtcggc cctagcctat gacccattgg caagtactaa cagcatgacg cagatggtgg
     2041 cggcaaaaaa tgctttgctg acggtcgatg gcatgagtat cagcaaatcc agtaatacgg
     2101 tgtctgatgt gattcaaggc gtgacattga cccttaaagc cgtgacgtcg acgagtaata
     2161 ccctctcggt aggcaccgac agcgatacca tacagagttc ggtacaaagt tttgttgatg
     2221 cctataatgc attgaatacc agtatgcgta acctgaccaa gtttgtgtcg gcaggttcga
     2281 gctctaatgg cgtgctgcta ggggattcaa cagcacgcaa tatcatggtt aagttgaagt
     2341 cgatgctatc ggcttcttcg ccgacggcga cgacctataa aacgctgtcg gatattggcg
     2401 tctccatggg ttcggatggc tcgttgtccc tggatagtac caagctgcag aaagctatta
     2461 ccaataacgt gagtgatgtt gccaaactgt tttcaccgtc tgccacatct accgatccgc
     2521 aagtcacgtt tgtcagcagc aaggatgata cggtttcagg cacttatgcc gtcaatatca
     2581 cacgattagg tggcaatggt gtcgatgcag ctgggaccat caatggagtg acagccatca
     2641 ccacgggctc catattgacc ggggcgggcg gggatgccag ctatggcttg caactcagtg
     2701 tcaccgggac tgcaaccggc agccgtggca cagtgacctt cagtaaaggc ctggcggggg
     2761 agcttaacag cttgctgacc tcttggctgg ataccgatgg tgccttggca acaaaaaccg
     2821 atggtattaa ctcttcgatt aaagatttga ataaaaaggc cgatgatatt aatgccaagc
     2881 tgccttctat tgaagcacgt taccgtgccc agtatgcaaa actggatgcc ttgctcagta
     2941 gtttgcaaag cactagtagc tctctgactt ctcaactggc tgccattagc gctaacggtt
     3001 agttaagtcg gtagtcgtct gcaagaagcg gctggcaagt aattttaaac catacgcttt
     3061 taagcagaat tcaggagtaa aaaatgttcg ggatgaaaaa atcaggtgtg aatgcttatg
     3121 caagcgtggg gttggaaacc ggcgatgtgg acgccagccc gctgaaattg atcgtgatgt
     3181 tatatgacgg cgcgatcact gcctgtatca aggcacaaca ggccatggca cagaatgata
     3241 ttgccaaaaa aggcgaatac ctgacccagg ccgtgacgat tgtggatagt ggcttgcgta
     3301 cctgcctgga caaacgtgct ggtgggctca ttgcccaaaa tctggatcag ctttaccaat
     3361 acatgacacg tagcctgatg gaagccagtg tgcgccaaga cgtgaaaaaa gtacaagaaa
     3421 ttcagcaatt gctgatggaa ttaaaagggg cttgggaaac actggaaaaa tctgcggtta
     3481 cccgcccagc acaagtagac atgtcattgg gccagatggt tgcccagaaa acacatgaat
     3541 tgcaacatat ggggcaagcc aaccgtttgg ccactgccgg agcttaagat gtcacaagca
     3601 ttgattaata cctatgaatc cgctgcaaaa ttgatggagc agatgctgat tgcggcgagg
     3661 caaaatgcct gggaaaaagt cacggagtta gagcagtctt acatgcttaa aatcgaagtg
     3721 gtgaaaaccc tggagcagaa tatgcgcctg gcatctgaaa agctgtcgcc agacttgcat
     3781 gcacgcaaac aggtgttggt gcaaaagatc atggccgatg atggcgagat ccgaaatttg
     3841 ctttatccgg tgatgcaccg gattacagac atgatttttg acgcgcaatt acatgcgcgt
     3901 attcccccgg atgtgaccta aatgctaccc atcccgcaag ttctcaccca acgttttatc
     3961 gccagtgaaa cagagagctt gcgcgcggtt tcttccattt tgcctgttga aggaacacaa
     4021 gcagaagcgc cattatggca gcgcatgcgt cctggcatgc aatttaccgg cttgatcgtt
     4081 cagaaagaag ctgcgggcga gaacggttca gagctggcac agttcaaagt tcaaatccaa
     4141 ctccctaatc aaccaccagc cctggtacag atgcagctgc ctgcctacct ggcacagcag
     4201 cagaccttga atatgcagtt tatggggctt gcccagcaaa gtaccggaaa agacgctttg
     4261 ccacaggtga aatggggatt gcagcagggt aacgccaatg ctggctcggc acgtttgggt
     4321 gatgcagcat tactgacgga tatgtcacag ccggagggca gtgagcattt gcaggacatg
     4381 gttaaaaccg ctgccgggca ggccagcctg gtcgagctga gtaatcctgc caagcatttg
     4441 cagcgctggc tgagctcttc ggagttccag caacaaacca atgcattaca ggcacaagcc
     4501 gtggtcagtc atagtccaca gaaaccgcag gtgctggcac aagacctcaa gcacgccatg
     4561 gataattccg gcctgtttta tgaatcccat ttaaaagaag cgaccgtagg cagccgcccc
     4621 tggcatcaac tgctgcagga gcctcagaac aagcccagtt ttgcaccacc ggaaatggtg
     4681 gcgcaacagt tacaagtgct agagcagcaa cgtgtggtct ggcacggcga agtctggcca
     4741 ggacaaacca tgcaatggca agtcaacgag caggaaagct cgccgcagtc gtcagaagag
     4801 ccggtattga acagtctgca aagtgacctg accttgcact tgccgcggct cggcgatgtc
     4861 aaagtgaata ttgccatggt gaatggccgt tttagtgtgc acatactgcc aagtgaatca
     4921 caagcgctca aaaccatgca gggcagtcgt agccagttag ccatgagcct gcagtctgca
     4981 ggcttgaaac tggatgcgtt gcagattacg cgcgatgagg cggatgaaga caggcggcaa
     5041 tatgcagcgc gataaggctt atacacaatc tgcggtggca ctggcctacg aagcaggaga
     5101 tcttgcccca cgcgtagtgg ccaaagggaa tggtttgctg gctgagcgta ttattgcgct
     5161 agcaaaagaa catgaggtat atgtgcatga gtcgcaggcc ttgttaagcc tgttgatgca
     5221 ggtagaactg gatgcacata ttccaccaca gctataccag gccattgccg agatattggc
     5281 ttggctgtat caactagagc agggagagtt gagggaaccg cctgcggtat aaggcagtct
     5341 caatttaatt ggctggtttg acgaacaagg tgtgacttgg gacagcaagc aaggttatac
     5401 ctgcatgttc atcatgtcct gataggcggt gaccagtttg ttgcggacct ggacggttgc
     5461 ctggaaagca atattggctt tttggcctga aatcatcaca tcagacaggc tgacattatc
     5521 attgcccatg gcaaagtcct gccccagctt ttctgattgc agctgcatat tattgacttg
     5581 gtccagcgat gccttgaggg catcactaaa gctgacctgg ctggtcgggc tcgtctcatt
     5641 ggttttttgt accggactag cgatagcgct gctgccaagc ccttcaggct tttgtgctgc
     5701 tgcacgtaac tgtgccagca tggacgtaat gcgttgagca tcaatgccgc ctgcgttcat
     5761 ggtggtctcc tttcgttgtc atgggcacat tcagcccttg gttgtcatgg tagatacttt
     5821 agcatcgcgt ggtatgaact gctttgcaaa ataggcaaca ttaatcggtc ttatttggca
     5881 atttgaaaat cttgagtaag cgcataatgt gatccatgtg agagtgtcct taaaaggcac
     5941 ttgagttcgg gaaagcgctt ttgaacctgt ccacccgttg tggacgtcgt tcagaagtca
     6001 tggtagtaaa catggaggtt cagatggctg ctgatgctgc cttacttacg tcaaatgatg
     6061 agtctaacgg gtcacccaat atgttcgggc aactaggaca aaagctgatg ctggtcgcag
     6121 gcattgcggc ggttgtcgcc gtgatggtcg tcttctggct ctggagccag aaaccggatt
     6181 accgtgtctt gttttccaat tttgccgata aagatggcgg cgccatcgtt gctgaactcg
     6241 ataagttgaa tgttccttac cagtttgctg aaggcggcaa tgctatcttg gtccctgccg
     6301 accaagtcca tcaagtgcgt ttgaaactgg ccgcacaggg cttgcctaaa ggcggcaata
     6361 ttggttttga gctgctagaa aaccagaagt ttggtgtatc ccagtttgtc gaacaagtga
     6421 acttccagcg tggcctggag ggagagcttg agcgtagtat ccaatccatt tcagcggtgc
     6481 aggctgcgcg tattcacctg gcgattccca agcctagcgt gtttgtccgt gagcagcagt
     6541 atccaacagc ttctgtcttg ctgaacttgc ataatggccg ccgcctggat gcacagcaag
     6601 tggctgccgt tgttcacctg gtggccagca gtgtgcctaa tttgtctgcg gaccacgtca
     6661 ccgtggtaga ccagaacggt aatctgttgt ctgacaacgc caataaaatc aagcaaaacg
     6721 gcttggatcc tgagcaaatg gcgtatgtgg aaaatatgca gaacagcatt gcccgccgcg
     6781 ttgaatcaat tatcacgcct atcgttggtg ccaagaatgt gcatgccgaa accagtgcgg
     6841 aaattgattt ctccatgacg gagcaggcca acgagtctta taaacccaat accaagcctg
     6901 atgacatggc tatccgtagt gcacaaagcc atgaagcgca aaatgccgct ggcagcagcg
     6961 gtgccgtgcc gggcgctttg tctaaccagc caccaggtga ttccactgcg ccaattaatg
     7021 caccaggggc acaggcgcct ggtgacaatg cagcggctgc ggcgccacca ccagcaccgc
     7081 cacaaaacac acaaaaagac acgaccacca attatgagct ggataaaacc gtcagctata
     7141 tccagcaggc aaaaggtggc attaaacgct tgcatgtcgc tgtggtggtc aaccatatcc
     7201 ctgtagtaga tagcacagga aaaacaactt accgcgcctt gagcaatgcc gagaagcaac
     7261 aagtgagtga tttggccatg caggcgatgg gcttcaataa agagcgtggg gataccattt
     7321 ctgtggtgaa taccccattt atcgctgaag cccaggagaa actggctgag ccgcctttgt
     7381 ggaaagatcc cctggtcatt gagtatggta aagatgcctt gcgcttcatt gccggtgtga
     7441 ttgtgctgat gatgatttac aaaaagctgc tcaagccaat ggcgaataag ctaacaggcg
     7501 cagacaagaa acagctggca ttggcaggta ctcctgcatt ggccggccct gatggcgcag
     7561 cggttgcggc tggcgctgat ggcgaagatg ccctggttac cctgagtggc gatgctactg
     7621 ccttgccgag tagcctggac caaacgctag aagcggccaa gcaagtggct aaagaaaatc
     7681 ctcgtatggt tgccaatgtg gtttccagct ggacatcggc ggagaaataa gccatgagtg
     7741 accagggatt aaccagaagt gcaattttaa tgctggcgct cggtgaggat gaagccgccg
     7801 aggtgatgaa gcatttgacc ccgaaagagg tgcagcgctt gggcattgtc atggcgtcca
     7861 tgaagccagt gcctcgcgaa gaagtggagc aggtgctgga aaagttcatg ctggactttg
     7921 ccagcagcag tgattttggc ctggattcag acagctacat ccgctcggta ctcattaaag
     7981 cgtttggcga tgataaagct tcaaacttgt tgaaccgtat tccgcaatcg caagatgcgg
     8041 caggcattga aagcctgaag tggatggatg cgttcagcgt ggctgatttt atcaagaatg
     8101 agcatccgca aattatcgcc actatcctgt cccatctgga ggctgagcag tctgcagaga
     8161 ttctggggca tttcactgaa cgtttgcgcc aggatgtgat tctgcggatt gcaacattgg
     8221 atagtatcaa gcccacagcc ttgcgcgaac tcaatgaagg catgctcaaa atgctggcgg
     8281 ggaatgacaa tatcaagcgt caggccattg gtggcgtgaa aacggctgcc gagatcatga
     8341 acttcatcag tggggaacat gaagccaaat tgatggatgg cctcaaaagt tacgatgatg
     8401 gcatggccca gcaaatcatg gaccagatgt ttgtgtttga caacatcatg gaaattgacg
     8461 acaggggcat ccaggtacta ttgcgcgaag tacagtccga catgctgatt gtcgccctga
     8521 aaggcacctc ggatgaaatg aaagaaaaat tcttccgcaa tatgtcttca agggcagcag
     8581 acatgatgcg cgaagacctg gaaagccgtg gcccggtcaa gctgtctgaa gtcgaaggcc
     8641 agcaaaaaca aattctgcag attgtgcgcc gccttgctga tgaaggccag attatgctgg
     8701 caggcaaggg tgacagcgaa cagtatgttt aaagcattca ataaggaaag tgtcatatgg
     8761 tagcttctgc aattccaaaa gaacaaatga gcgcctatca acgctgggaa atggccagct
     8821 ttgatgactc tgattccagt gagcgtgtca aacgcgagcg tgcgcaagcg gaagaaaatg
     8881 cacgcacagt cagccagatt ctcaaacagg tccgccagga agcctatgaa gaaggattca
     8941 agaccggata tgttgatggc atggggcatg ctgaaaaaca gttaagcgcc gaacgcgaac
     9001 agctcttaca aatggccggt aatttccagc aagccttgtc catgcaggat gtgagtgttg
     9061 ccgaatctgt cttaagcctg gcgctggatc tggcaaaagc catgatgaaa accaaatt
//
LOCUS       DATMSK010000184         4735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_273064,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000184 DATMSK010000000
VERSION     DATMSK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273064"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VNZ84_06050"
     CDS             complement(<1..403)
                     /locus_tag="VNZ84_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HWU66555.1"
                     /translation="MSRVWFLSSLLAYCANCHAQPVLEKVLDGDTVIIREEAHSYHLR
                     LLDIDAPELHQDFGKQSKRSLAQLCLNAHISVQPKGQDRYGRTLGYLYCGNTDASQSQ
                     VALGMAWFNQRYSNRTTLSALQQQAQQQGLGL"
     gene            complement(404..2014)
                     /gene="nadB"
                     /locus_tag="VNZ84_06055"
     CDS             complement(404..2014)
                     /gene="nadB"
                     /locus_tag="VNZ84_06055"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HWU66556.1"
                     /translation="MNKVFDVLIIGSGLAGQSLALRLADQHRVCILSKRELSLSASNW
                     AQGGIAAVLNSEDSVEAHIQDTLDAGAGLCDSEVTRMVVTNGRTSIEWLLQQGVNFTR
                     EAEDDRLHLTREGGHSHRRVVHVADATGQAVQTTLTDRVREHPNITILEHHIAVDLIT
                     THKLFKQTANDGPNRCLGAYVLNNQTGKVITLSAQYTVTATGGAGKVYLYTTNPDIST
                     GDGIAMAWRAGCRVANLEFVQFHPTCLYHPHAKSFLISEVLRGEGGLLLLPDGTRFMP
                     WHDERAELAPRDVVARAIDFEMKKRGLDCVYLDISHKPAEFIQSHFPNIYQRCLQLGI
                     DITKQPIPVVPAAHYTCGGVMTDMHGRTDIDCLYAIGETACTGLHGANRLASNSLLEC
                     LAFSQSCAQDIETLGAQPTISLPDWDESRVTDADEEVLITHTWDELRRFMWNYVGIVR
                     TTKRLSRALHRIHMLRDEVGEFYSNFKVSHDLIELRNLLQVAELIVLSAIERHESRGL
                     HYSKDYPDLLPEAIPTVLTPDNYTSLLD"
     gene            2224..2853
                     /gene="rpoE"
                     /locus_tag="VNZ84_06060"
     CDS             2224..2853
                     /gene="rpoE"
                     /locus_tag="VNZ84_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HWU66557.1"
                     /translation="MTVQAANAKQQENKEFDQMLVERAQQGDKRAFGLLVEKYHRKLG
                     RLLSRMIRDQAEVEDVVQESFIKAYRALHSFRGDSAFYTWLYRIGINTAKNYLVSMGR
                     KPQVMQDVEIEDVENFDEGNDMRTMETPETSLMTKEIAQTVNDAIAALPEELRTAITL
                     RELEGLSYEDIANVMQCPIGTVRSRIFRARETIATKLRPLLDTPQHKRW"
     gene            2941..3411
                     /locus_tag="VNZ84_06065"
     CDS             2941..3411
                     /locus_tag="VNZ84_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HWU66558.1"
                     /translation="MKQQLSALVDGEVDIERCEHIFLAAKSSGEVAQAWRDYHVIRDV
                     LREEGWVQSDMTSRIMAQLADEPTMLAPQAVAASEKISHIRPRFSKYTWSIAASVAAA
                     FFVGLFVVNQPEVTAPAQIADNDADTDQYVLAHHNYAPNSATYYMHNVAYNAGE"
     gene            3434..4402
                     /locus_tag="VNZ84_06070"
     CDS             3434..4402
                     /locus_tag="VNZ84_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HWU66559.1"
                     /translation="MSLKLVFRGYFLPVLLLAVTSQPVVAEEDLWATMQKTASAAREL
                     NYQGLFVYQNGAVIRSVQITHIYEGGREFTRNVVLDGRPREVFSEGDDIVIFNAKNDK
                     VVIEKRRGQNLFPAMLPTQLYPLKQNYKLKFVGQERVAGRLTQVLDMIPNDNLRYQYR
                     IWSDSEYGLLLKMSMMDVQGHTLEQIGFNQVSMLQTKDLDWFQPKIDATKPYVMDDTT
                     DLTHTVENLVIPNLPAGYKKIDQIQLKVPGRTTTVQQLIFSDGLASVSLFVEPMIKGM
                     QPRIGKKNMGSTNVYAHVLNGYQLVVVGEVPAQTVEQIAQQVTFKK"
BASE COUNT         1196 a         1096 c         1258 g         1185 t
ORIGIN      
        1 ataagcccag gccttgttgt tgggcctgct gttgcagcgc tgacagtgtg gtgcggttgg
       61 agtagcgctg gttaaaccag gccatcccta aggcaacttg tgattgactg gcatcggtat
      121 tgccacagta tagataccca agtgtcctgc cgtagcggtc ttgtcctttt ggttgcacgg
      181 aaatatgtgc attgaggcaa agttgtgcaa ggctgcgttt agattgtttg ccaaagtctt
      241 gatgcagctc aggcgcatca atatccagca ggcgtaagtg ataggagtgt gcctcctccc
      301 gaatgatgac agtgtcgcca tcaagcactt tttccagtac gggctgtgca tggcagttgg
      361 cgcagtatgc gagcaggctg ctcagaaacc agactcggct catctaatcc agcaggctgg
      421 tgtagttgtc cggtgtgagc acggtaggaa tcgcttccgg caacaagtca gggtagtctt
      481 tgctgtagtg caggccacgg ctttcatggc gctctattgc actgagtacg atcagctccg
      541 ccacttgtag caagttgcgc aattcaatca ggtcgtggct gactttaaag ttgctgtaaa
      601 actcgccgac ttcatctctg agcatatgga tacggtgcag ggcgcggctc aggcgcttgg
      661 ttgtgcgcac aatacccacg tagttccaca taaaccgacg taattcatcc caggtatgag
      721 taatcaaaac ttcttcgtca gcatcggtga cacggctttc atcccagtca ggaagtgaga
      781 tggtgggttg tgcacctagc gtttcaatgt cctgtgcgca gctttggcta aatgcgaggc
      841 attccagtaa ggagttactg gccaggcggt ttgcaccatg caatcctgtg caggcagttt
      901 caccaatggc atacaggcag tcaatgtcag tgcggccatg catgtcagtc atgacgccac
      961 cacaagtata atgtgcagct ggaaccacag gaatcggctg cttggtgatg tctatgccca
     1021 attgcaggca gcgctggtaa atattgggga aatgagactg gataaattct gccggtttgt
     1081 ggctgatatc taggtagaca cagtcaaggc cgcgcttctt catttcaaag tcgatggcgc
     1141 gtgcaaccac atcgcggggt gccaattccg cacgctcatc atgccagggc ataaaacgcg
     1201 taccatcagg caataacagc aaaccacctt caccacgcaa cacctctgaa atcaaaaacg
     1261 atttggcatg cgggtggtaa aggcaggttg ggtgaaactg tacaaactcc agattggcga
     1321 cgcggcaacc cgcacgccag gccatggcga taccgtcgcc ggtgctgatg tctggattgg
     1381 tggtatacaa atagaccttg ccagcaccac cggtcgccgt gaccgtgtat tgcgccgaga
     1441 gtgtgatgac cttgccggtc tggttattga gcacataggc gcccaggcag cggttagggc
     1501 catcattagc cgtttgcttg aacagtttat gcgtggtgat caggtccacg gcgatgtggt
     1561 gctcaagaat ggtgatgttg gggtgttctc gtacacggtc tgtgagtgtg gtctgcacgg
     1621 cttgcccggt ggcatccgca acatgaacga cacgcctgtg actatgtcca ccctctcgcg
     1681 tgagatgcag acggtcatct tcagcttcgc gggtaaagtt cacgccttgt tgtagtaacc
     1741 actcaatact ggtgcggcca ttggtgacca ccatgcgagt gacctcgctg tcacataatc
     1801 ccgctcccgc atccagcgta tcctggatgt gagcttccac tgagtcttca ctattcagta
     1861 cggcggcaat cccgccttgc gcccagttgc tggcgctcag ggatagctca cgtttgctga
     1921 gtatgcatac tcgatgctga tcggccaagc gtaaggccag tgattggcct gccaggccgc
     1981 taccgataat caggacatcg aacactttat tcatattctg gtggagttaa tggaacttcg
     2041 atggggatgg ggtgtcacat aaattgccag cattgcatat tgacgttgca ggcaaaaata
     2101 agggatgaga cgatgttaca cacagcaaat ttagaggcgt taacacgcac cgggttcgtc
     2161 gaaaataaca caaaagcgta tactactccc aatcataaaa ttacccaagg gagtcatcgc
     2221 gatatgactg ttcaggcggc gaacgccaaa cagcaagaga ataaagagtt cgaccaaatg
     2281 ctggtcgaac gtgctcagca aggcgacaag cgtgcctttg gactattggt ggaaaaatac
     2341 catcgtaagc ttggacgcct attgtcgcgc atgatccgcg accaggcgga agtggaagat
     2401 gtggtgcaag agtcctttat caaggcctat cgtgcattgc acagcttccg tggtgatagt
     2461 gccttttata cctggctgta ccggattggc attaatacgg ccaagaatta cttggtttcc
     2521 atgggtcgca agccgcaggt gatgcaggat gttgaaatcg aagatgtaga aaacttcgat
     2581 gaaggtaacg atatgcgcac catggagaca ccagaaacct cgttgatgac caaagaaatt
     2641 gcgcaaacgg tgaacgacgc cattgcggcg ttgccagaag agttgcgtac tgcaattaca
     2701 ttgcgcgagc ttgaaggatt gagttatgaa gacattgcta acgtgatgca atgtccgatt
     2761 ggtactgtac gatcacgtat tttccgggcg cgagaaacaa ttgcgaccaa acttagacca
     2821 ttgctggata cgccacagca taaacgctgg tagtggtatc cacagtaaaa cgatgacggg
     2881 tgttatgaca aattttttgt taaatgctaa cataaaaaca ttgatggatt tggggttatt
     2941 atgaagcagc aattatctgc cttggtcgac ggtgaggtcg atatcgagcg ctgtgaacat
     3001 atttttctcg cagctaagtc tagtggagag gtcgcgcagg cttggcgtga ttatcatgtg
     3061 atccgggatg tgttgcgtga agagggttgg gtccaatctg atatgaccag ccgcattatg
     3121 gcgcaattgg ccgatgagcc caccatgctg gccccgcagg cggtcgcggc atccgaaaag
     3181 atttcccata tccgtcctcg tttcagcaag tatacgtggt caattgccgc ttcagtggcc
     3241 gctgccttct ttgtcggcct gttcgtggtc aaccaacccg aagtgactgc tccggcacag
     3301 attgcggata acgatgcaga tactgatcaa tatgtattgg ctcatcataa ctatgcccct
     3361 aatagcgcca catactacat gcacaatgta gcgtataacg ctggcgaata agtctattgt
     3421 tgtttacggg tatatgtcac tcaagcttgt ttttcgtggg tatttcctgc ctgtactatt
     3481 gctggctgtc accagtcaac ctgtggttgc cgaggaggat ctctgggcaa ccatgcagaa
     3541 aaccgcttct gcagcccggg agctcaatta ccagggattg tttgtctacc agaatggagc
     3601 ggtgatacgc tcagtccaaa ttacccacat ctatgaaggt gggcgtgaat ttacgcgtaa
     3661 tgttgtgctg gatggcaggc cgcgtgaagt gtttagcgaa ggcgatgata tcgtcatttt
     3721 caatgccaag aatgacaagg tcgtcatcga aaaacggcgc gggcagaact tgtttcctgc
     3781 catgcttccc acgcaactgt atccgctcaa gcaaaactac aagctgaaat ttgttggcca
     3841 ggaaagagtg gccggacggc tgacgcaagt cctggatatg attcctaatg ataacctgcg
     3901 ctatcaatac cgtatctggt ctgatagtga gtatggtttg ctgcttaaaa tgagcatgat
     3961 ggacgtgcag gggcataccc tggagcaaat aggcttcaac caggtgtcta tgctacagac
     4021 caaggacctt gattggttcc agccgaaaat tgatgccacc aagccttatg tcatggatga
     4081 caccacagat ttgacccata ctgtggaaaa cctggtcata cctaatctgc ctgccggtta
     4141 caaaaaaata gatcaaatcc agctaaaagt gccgggaagg acgactacgg tacaacaact
     4201 cattttttct gatggtctgg cgtccgtatc gctgtttgtc gagccaatga ttaaaggtat
     4261 gcagccgcgc ataggtaaga aaaacatggg ttcaactaac gtttacgcgc atgtgttgaa
     4321 tggttatcag ctggttgtgg tgggtgaagt gcccgcgcaa acagtagaac aaatcgcgca
     4381 gcaagtgact ttcaagaagt agtttgctgt gtcataaaga atatgcagat aaattgctat
     4441 gattcaaact gcattcgttt ccatgacttg tatttgcaaa tacaagtcgc caattaatgg
     4501 gtgaaaagtt tttgtgacta ccactcaaac ctccgagtag agtgcaactt ttaattttta
     4561 atagtcatta aatgatagga ataatcatga tgagacaatg gatttctggc ttgatgatgg
     4621 ctagtgcaat cacgctggct gcaccggcaa ttactgccca agcaaaggat ttgcctgatt
     4681 ttacagagct ggctgaaaaa cagggcccgg cagtagtgaa tattagtatt acttc
//
LOCUS       DATMSK010000185         8376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_460639,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000185 DATMSK010000000
VERSION     DATMSK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460639"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2923
                     /locus_tag="VNZ84_06075"
     CDS             <1..2923
                     /locus_tag="VNZ84_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU66560.1"
                     /translation="TIQVIFDLGVDPNAAMVLVKTRLDQVMSKLPPLVQLEGVIVNTV
                     QPSMLMYVNLYSKDKNADEKFLFNYVNVSVIPEIQRVHGIAQAQNLGSRQFAMRIWLN
                     PERMRAYKVSTKEVMDAISSQSVIGRPGRLGQSSGIYAQSKEYVLVYKGRYNKPEEYG
                     DIIIRADSNGEILHLKDLAKVELGSEFFDIYSNKDGYPSAAIVLKQNYGTNASEVIAD
                     VKTKMEELKQSFPAGMDYEINYDVSKFLDASIEKVIHTLGEAFVLVALVVFIFLGDWR
                     STLIPTLAVPISLIGAFFVMKSFGLSINLVTLFALVLAIGVVVDDAIVVVEAVHAKMA
                     SDHVGPYKAVQRVLGEIGGAIVAITLLMTSVFIPITFMPGPVGVFYRQFAITMASSIV
                     LSGIVALTLTPVLCAMILKDTHGQPKRKTPISLFLDGFNWVFEKVTGRYTNFLEKVVT
                     LRTVTFIVLGLFSAGIFYVDKVLPAGFIPGEDQGMIYAIIQTPPGSTLETTNKVARHL
                     QSLAKQIEGVESVSSLAGYEVLTEGRGSNAGTCIINLKDWSERKQSVKQIIEELEEKT
                     KNFGAVVEYFQPPAVPGYGAASGLSLRLLDKTTTADYYVFDKLNKEFMAALGKRKELT
                     GLFTFYAANYPQYELIIDNQLAMQKKVSIEDAMNNLDILIGSTYEQGFIRFNNFFKVY
                     TQAGPEFRRYPSDVLNYFVKNEDGEMVPYSAFMTLKKRQGPNEITRYNLYNSAAIRAE
                     PAPGYTTAAAIAAVKEVAKATLPKGYDIAWEGLSYDEEKRGNEALMIFLVVLGFVYLV
                     LAAQYESFILPFAVILSLPAGIFGSLFLLKILGLANDIYAQVGLIMLIGLLGKNAVLI
                     VEFAVQKRGQGMSIRDAAIEGARTRFRPILMTSFAFIAGLIPLVIATGAGAVGNRTIG
                     TSALGGMLFGTIFGVIVIPGLYYIFGSLADGKALIRKEETEPLTETYHYNIHQDDDDE
                     E"
     gene            2913..4376
                     /locus_tag="VNZ84_06080"
     CDS             2913..4376
                     /locus_tag="VNZ84_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWU66561.1"
                     /translation="MKNKQLQWMVAGAVSLMLQSCAIPFITSKQADTTLPTQYQETVA
                     PAGNSASVKWQDFFEDQNLVSLIDTAVNNNKEIHIMAQRITVAQNEIQARKGEYLPFV
                     GIGASADKEKVGKYTRNGAVEENLDIKEGRSNPRFLGNYQFGLVASWELDVWKKLRNA
                     TKVAVMEYMATIEGKNFLVTNLVAEVAHAYYELIALDNELITLDQNIKIQQNALEVVK
                     QLQQYARANSLAVKRFEAEVQKNQSRQYVLKQSIVETENRINYLLGRTPQPITRASAN
                     FMSMQNKVVQTGIPSELLANRPDIRQAELELSAAKLNIDVAKANFYPSFSIRAGLGFQ
                     AFDPKYLLNVPASIMAGFGGEMIAPLINRNAIEAQYKSANAVQIQAAYDYEQKIINAY
                     MEVANQIANIDNLDKNYQLKNKQVEALTQSIDVANQLFKAARADYMEVLLTQRDALDA
                     KMELIETKQQQVAAVVDLYRSLGGGWQTAETAAPAKP"
     gene            complement(4439..4801)
                     /locus_tag="VNZ84_06085"
     CDS             complement(4439..4801)
                     /locus_tag="VNZ84_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HWU66562.1"
                     /translation="MRDLDQLFQTLQRSSFRNSFHLNHRDLHYLQQKGLPVILDHAEQ
                     FIAERLAPAEIANDGQQTPMRGHPVFVAQHATATCCRGCLSKWHRLPAGRALTLEEQA
                     YIVVVIERWLQRYLSAPE"
     gene            complement(4807..6039)
                     /locus_tag="VNZ84_06090"
     CDS             complement(4807..6039)
                     /locus_tag="VNZ84_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU66563.1"
                     /translation="MSLSVRQILAHQGFAFPKLLTYRIFMVLSYQMMMVAIGWHIYEL
                     THDPWSLGLIGLAEVIPYMGSALFAGHAVDHYFSRRGFAVVSALFLAVNAGLLVWITQ
                     HQGPHTANLIYGTVALTGFARALISPSYNTLFALIIPRASIAKASGIGSSMFQIGLIV
                     GPAIGGLLVGFADKSVAYEISAVLALGGAIAALGLRVKEYRSHTPMRVFASIGEGLRF
                     VRRTQVVLGAQLLDMFAVLFGGAVAMLPAFVHDIYQMGPEGLGVLRAAPAFGAIVTGL
                     WLAKHPIHEHSGRWLLFAVAGFGVCMIAFGLCRTYWLGIVILAVSGMMDAVSVILRQT
                     ILQLATPDGMRGRVSAINGIFIGSSNELGAFESGLTSRLMGLVPSVIFGGCMTILVVG
                     VTAFKAPKLRDLHLSQLH"
     gene            6293..7531
                     /locus_tag="VNZ84_06095"
     CDS             6293..7531
                     /locus_tag="VNZ84_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWU66564.1"
                     /translation="MADYLELRRQLYYAVLTVPKPLQSELGIRFVQSTGTGDRRRAKD
                     IAAQLVSNWKVQIEQARGRDTSTLSKAIQWRRELEKAKSDDLKETITTLITDEAERLH
                     DTTGSVDTAVQFVAIATGAKTLAEPYLSQWKAQLTLTPRTVEQYSKDALLFVQRFPFI
                     EDVSKPKVSLWLDTLATQGVSKDSQKRIIKGCRSFWKFVYRYGIKGMPEDPFHKVITT
                     DKTKKKRPRETFEPQEIVTLWQMAKDRSDDLLADLIAMGAYTGARIEELCTIKVSDVT
                     EDTIKINDSKTSAGIRSIPIHRRIRPVIERLKTTSTDGYLMSGLTFDKYLDRSGAMCK
                     RFGKLKTSAGHGRTKVFHCLRHTLITTMVNAGVSEFVIQDIVGHEKKGVTGQSYVKTI
                     KHEVKLEAIERVAYPFPESV"
     gene            7648..8016
                     /locus_tag="VNZ84_06100"
     CDS             7648..8016
                     /locus_tag="VNZ84_06100"
                     /inference="COORDINATES: protein motif:HMM:NF039385.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HWU66565.1"
                     /translation="MEETNFFSQDNVSVSNSRFIVNGQTYAMSNVTSVKSGVTPPNRG
                     AAILLILVGLLCFLGSGTAYIIGFGLIFAGAIAWSNAKAQYSVILNTSAGENQALTSE
                     DSLYIEKVIHALNAAIVSRG"
     gene            8150..>8376
                     /locus_tag="VNZ84_06105"
     CDS             8150..>8376
                     /locus_tag="VNZ84_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66566.1"
                     /translation="MESQKLQLEIQNLKKAQEKSESRILIATTEETKEYNQPAFSRTG
                     AYQDDYANFSAGNSSKSRASKTPLLDYGIMV"
BASE COUNT         2231 a         2148 c         2158 g         1839 t
ORIGIN      
        1 aaccatccag gtgatttttg acctgggtgt ggatccgaat gcggccatgg tgctggtcaa
       61 aacccggcta gaccaggtga tgagtaaact ccctcccttg gtgcagttag agggggtgat
      121 cgtcaatacc gtacagccga gcatgctgat gtatgtgaac ctgtacagta aagataaaaa
      181 cgccgacgag aaattcctgt ttaactatgt aaacgtcagc gtgattcctg aaatccagcg
      241 tgtacacggg attgcccaag cgcaaaacct gggtagccgc cagttcgcga tgcgtatctg
      301 gctcaaccct gagcgtatgc gtgcctacaa ggtatcgact aaagaagtca tggatgcgat
      361 cagcagccag agtgtgattg gtcgccctgg ccgcctgggc caaagctcgg ggatttacgc
      421 ccagtccaag gaatatgtcc tagtgtataa gggccggtat aacaagcctg aagagtatgg
      481 tgacatcatt atccgcgccg attccaacgg cgagatcctg cacctgaaag acctggccaa
      541 agtcgaactt ggcagtgaat tctttgacat ttactccaac aaggacggct acccatccgc
      601 cgcgattgtg ctcaagcaga actatggtac caacgccagt gaagtgattg ctgacgtgaa
      661 aaccaaaatg gaagagctga aacagagctt tccggccggc atggattatg aaatcaacta
      721 tgacgtctcc aagttcctgg acgcatcgat tgaaaaagtg atccatacct tgggcgaagc
      781 gttcgttctg gtggccctgg tggtatttat cttcctcggt gactggcgct ccacgctgat
      841 accgaccctg gcggtaccga tctcgctgat tggcgcgttc tttgtcatga agtcgtttgg
      901 cctgagtatc aacctggtca cgctatttgc cttagtcctg gcgatcggcg tggtggtgga
      961 tgatgcgatt gtggtggtgg aagcggtgca cgccaagatg gcctcggacc acgtcggccc
     1021 gtataaagcg gtacagcgtg tgctgggtga aattgggggt gccatcgtcg cgattaccct
     1081 gctgatgacc tcggtattta ttccgatcac ctttatgcct ggcccggtcg gtgtgttcta
     1141 ccgccagttt gccatcacta tggcctcctc catcgtgctg tccggtattg tggcattgac
     1201 actgacaccg gtgctttgcg ccatgatcct gaaagataca catggtcaac ccaaacgcaa
     1261 aacgccgatc tcgctgttcc tggatggctt taactgggtg tttgagaagg taaccggccg
     1321 ctacactaac ttcctggaaa aagtggtcac gctacgtaca gtcacattta ttgtactggg
     1381 gctgttctca gccgggattt tctatgtcga caaagtcctg ccggccgggt ttatccccgg
     1441 tgaagaccag ggcatgattt acgccattat ccagacgcct cctggctcta cgctggaaac
     1501 caccaacaag gtggcacgtc acctgcaatc gctggccaag cagattgaag gtgtggaatc
     1561 agtatcttcc ctggcgggtt acgaagtact gaccgagggc cgtggctcta atgcaggtac
     1621 ctgtattatc aacctgaaag actggtctga gcgcaagcaa tccgtgaagc agatcatcga
     1681 agagctggaa gaaaaaacca aaaactttgg tgcagtggtg gagtacttcc agccaccagc
     1741 ggtgcctggt tacggtgcgg cttccggttt gtcgttgcgt ttgctggata aaactaccac
     1801 ggctgactac tatgtgtttg ataaactcaa caaggagttc atggcggcat taggcaagcg
     1861 taaagagctg actggcctgt ttacgttcta tgcagccaac tatccgcagt atgagttgat
     1921 tattgacaat cagctggcca tgcagaaaaa agtgtctatc gaagatgcca tgaacaacct
     1981 ggacatcctg atcggtagta cctacgagca aggttttatc cgctttaaca acttcttcaa
     2041 ggtctacacg caggccgggc ctgagttccg ccgttacccc agtgatgtac tgaattactt
     2101 cgtgaaaaac gaagatgggg aaatggtgcc ttactcagcc tttatgacac tcaagaagcg
     2161 tcagggtccg aacgagatca cccgttacaa cctgtacaac tcagcggcga tccgggcaga
     2221 acctgcccct ggctacacca cggctgcagc gattgctgcg gtgaaggaag tcgccaaggc
     2281 cacgctcccc aaaggctatg acattgcctg ggaagggctg tcctatgacg aggaaaaacg
     2341 cggtaatgaa gccttgatga ttttcctggt ggtgctgggc tttgtctacc tggtgctggc
     2401 ggcgcagtat gagagcttta tcctgccatt tgcggtgatc ctgtccttgc ctgccggtat
     2461 ttttggttcg ctgttcctgc tgaagatact ggggctggcc aatgacattt atgcgcaggt
     2521 gggcctcatc atgctgattg gcttgctggg taagaacgcg gtgctgattg tggagtttgc
     2581 ggtacagaaa cgcggtcaag gcatgtctat acgcgatgcg gccattgaag gtgccagaac
     2641 ccggttcagg cctatcctga tgacctcgtt cgcctttatt gccggcctga tcccactggt
     2701 gattgccacc ggtgccggtg ccgtgggtaa ccgtacgatt ggtacgtcgg ccctgggtgg
     2761 tatgttgttc ggtaccattt ttggcgtcat cgtgatccct ggcctctact acatttttgg
     2821 ttcgctggct gatggcaaag cactcatccg caaggaagag accgagccgc tgaccgaaac
     2881 ctatcactac aatatccatc aggatgatga tgatgaagaa taagcaattg caatggatgg
     2941 tcgctggtgc agtgtcgttg atgctgcaat cgtgcgccat cccatttatc accagcaagc
     3001 aggcggacac cacactcccg acgcaatatc aggaaacggt ggccccggct ggcaatagcg
     3061 ccagtgtcaa atggcaggac tttttcgaag accagaatct ggtctcgctg atcgacactg
     3121 ccgtgaataa caacaaggaa attcatatca tggcgcaacg aatcacggtt gcgcagaatg
     3181 aaatccaggc ccgcaagggt gaatacttac cgtttgtcgg catcggcgcc agcgccgata
     3241 aagagaaggt cggcaagtac acccgtaacg gtgcggttga agaaaacctg gacatcaagg
     3301 aaggccgctc caaccccaga ttcctgggca actaccagtt tggcctggta gcctcctggg
     3361 agctggatgt gtggaagaaa ctgcgtaatg cgaccaaagt cgccgtgatg gaatacatgg
     3421 cgaccattga aggtaaaaac ttcctggtca ccaatctggt ggccgaggtg gcgcatgcct
     3481 actatgaatt gatcgcgctg gataacgagc tgatcacgct ggaccagaat atcaagatcc
     3541 agcaaaacgc gctcgaagtt gtgaagcagt tacagcaata tgcacgtgct aactcgctgg
     3601 cagtgaaacg gtttgaagca gaagtgcaga aaaaccagag ccgccagtat gtgctgaagc
     3661 aaagtattgt tgaaaccgaa aaccgcatca actacctgct gggtagaacg ccgcaaccga
     3721 tcacacgggc ttcggcaaat ttcatgtcca tgcaaaacaa ggtggtgcag acgggtatcc
     3781 cttcagagct gctggccaac cgcccggata tccgccaggc ggagttggag ctctcggcag
     3841 ccaagctgaa tattgatgtg gccaaagcca atttctatcc gtcattctcg atccgggcag
     3901 ggctgggctt ccaggcgttt gatcctaaat acctgctcaa cgtcccggcg tccatcatgg
     3961 ctggctttgg cggcgaaatg atcgccccac tgatcaaccg gaatgccatc gaggcacagt
     4021 acaagagtgc caatgcggtg caaatccagg cagcgtatga ctacgagcaa aagatcatta
     4081 atgcctacat ggaagtggct aaccagattg ccaacattga caacctggat aaaaactacc
     4141 agctgaaaaa caagcaggta gaagccttga cgcaatccat tgatgtggcc aaccagttat
     4201 ttaaagccgc ccgcgcagac tacatggaag tgttgctcac gcagcgtgac gcactggacg
     4261 ccaaaatgga gctgatagag accaagcagc aacaggtcgc ggccgtagtg gacttgtatc
     4321 gctcactcgg cggcggctgg caaacggctg aaacagccgc acccgccaaa ccctaaagac
     4381 aacgtgtctg atcaggagaa gtgcccatgg ccttctcctt ttttttattt ctaacaccct
     4441 actctggcgc agataagtag cgttgcaacc agcgctctat gaccacaacg atatacgcct
     4501 gctcttccag agtcagtgcg cgccctgcag gtaaacgatg ccatttggat aagcagccgc
     4561 ggcagcaggt ggcggtggca tgttgcgcaa caaagaccgg gtggccgcgc atgggcgttt
     4621 gctgaccgtc attggcaatt tctgcgggtg ccagccgttc ggcaataaac tgttcggcat
     4681 ggtccagaat gactggcaaa cctttttgct gaaggtaatg caggtccctg tgattgaggt
     4741 ggaagctgtt acgaaaactg gaacgttgca gcgtttgaaa caactggtcc aggtcacgca
     4801 tggcatttaa tgcaactgcg agagatgcag gtcacgtagt ttgggcgcct tgaatgccgt
     4861 cacaccgacc acaagaatcg tcatacagcc gccaaaaatc accgatggca ccagccccat
     4921 cagccgggag gttaagccag actcaaacgc tcctaattca ttcgacgatc cgataaaaat
     4981 gccattaatc gctgaaacac gccctcgcat gccatcaggg gtggccaact gcaaaatggt
     5041 ttgccgtaag atcacagaca cggcatccat catgccggaa accgccagga tcacaatccc
     5101 taaccagtag gttcggcaca aaccaaacgc aatcatgcac acaccaaagc ccgctactgc
     5161 gaacaataac cagcgtccac tgtgctcgtg aatcgggtgt tttgccaacc agagacccgt
     5221 cacaatcgcg ccaaaagccg gtgcggcacg caatacgccc agcccttccg gacccatttg
     5281 gtaaatatca tgcacaaaag cgggcaacat ggcgacggca ccaccaaaca ggactgcaaa
     5341 catatccagc agctgcgctc ccagtaccac ctgcgtacgc cgcacaaaac gcaagccttc
     5401 gcctatgctg gcaaataccc gcataggcgt atggctacgg tactctttaa cccgcaaacc
     5461 cagcgcagca atggcgccac ccaaagcgag taccgcagaa atttcgtatg ccacagattt
     5521 gtctgcaaac ccgaccagca agccaccaat cgccggaccc acaatcaggc caatctggaa
     5581 catggagcta ccgatgcctg aggctttggc aatggaggct cgcggaataa tcaaggcaaa
     5641 cagggtgttg taactaggtg aaatcaacgc acgtgcaaag ccggtgagcg cgacggtgcc
     5701 ataaatcaag ttagccgtgt gtggcccttg atgctgcgta atccacacca gcaaaccggc
     5761 attcaccgct aagaacaatg cagaaaccac cgcgaatcca cgccgggaaa aataatgatc
     5821 cacggcgtgg ccggcaaata aggcactccc catgtacggg atcacctcgg ccaggccgat
     5881 caggcccaaa gaccaagggt catgcgtcag ttcataaata tgccaaccaa tcgcaaccat
     5941 catcatctgg taggaaagca ccatgaaaat acggtaagta agcaacttgg ggaaagcgaa
     6001 accctgatgg gctagaatct ggcggacgga taaggacatg gctaggatta tacgctatca
     6061 gcctgcatgg cagattgtcg cggaatcccc tacaatccgt ctgcgggtgc agtgaatcag
     6121 gcagtaggcg aaagctgatg agccgtcgtt gacctcgtgc gactctgccg ctatagatga
     6181 ggcgcacccg ccctccttgt attcagccta gtataacact ttaaaggcga gaccgtgtgg
     6241 taaaaacgtg tggtaaaatt acaattaata tctcacacaa taagaagact caatggcaga
     6301 ctacctagag ttaagaaggc agctttacta tgccgtccta accgtcccca aacccctcca
     6361 aagtgagcta ggaatccgct ttgtgcaatc cactggcaca ggcgatagga ggcgcgcaaa
     6421 ggatattgca gcgcagcttg tgtcgaactg gaaggtacag attgagcaag cccgaggccg
     6481 tgacacctcc acactcagca aggccataca atggaggcgc gagcttgaga aggctaagag
     6541 tgatgacctg aaggaaacta taacaaccct tatcaccgat gaagcagaga ggctacacga
     6601 caccacaggg tctgtagata ccgccgttca atttgtcgcg attgccactg gagccaagac
     6661 acttgccgag ccatacctaa gccaatggaa ggcacaactc acgcttacac ctcgcaccgt
     6721 ggagcagtac agcaaggatg cgttactctt cgttcagaga ttcccattca ttgaggatgt
     6781 ctccaaaccc aaagtatcgc tatggctgga caccctagcc acccaaggag tctccaaaga
     6841 ctcacagaag cgaatcatta agggttgccg gagcttctgg aagtttgtct atagatacgg
     6901 catcaagggc atgcctgaag accccttcca caaggtcata actactgaca agacgaaaaa
     6961 gaaacgccct agagagacgt ttgagccaca ggagatagtt accctttggc agatggctaa
     7021 agaccgctct gacgacctcc ttgcagacct gattgcgatg ggcgcataca ctggcgcaag
     7081 gattgaggag ctatgcacaa tcaaggtgag cgatgtcacg gaagacacca tcaagattaa
     7141 tgactctaag acatccgcag gcattcgctc cattccgatt caccgccgta tcaggccagt
     7201 gattgaacgc ttaaagacca ccagcacaga cggttatctg atgtcaggac ttactttcga
     7261 taaatacttg gataggtctg gtgccatgtg taaacgcttt gggaagctta agacaagcgc
     7321 aggccacggc aggacaaagg tattccattg cctaaggcac accctaatca cgaccatggt
     7381 aaacgctgga gtgagtgagt ttgtcattca ggatattgtc ggccatgaaa agaaaggcgt
     7441 gacaggccag agctacgtta agaccatcaa gcatgaggtg aagcttgagg cgattgaaag
     7501 agtggcttac ccgtttcctg aatccgtgta acttattatt tacaccccac tgccgcaatg
     7561 gtttaattgg gagataactt agaacgagca agctgttctt tccgaatcag tatctttgct
     7621 attaaaaaca acaagggagt ggatgcaatg gaagaaacaa atttctttag tcaagacaat
     7681 gtaagtgtta gtaatagtcg ttttatcgtc aacggacaaa catatgcaat gagcaatgtt
     7741 acatcagtaa aatctggcgt tacccctcca aatcgaggag cagctatcct cttaatctta
     7801 gtaggtctac tctgctttct tggctctggt accgcttaca ttattgggtt tgggctgatt
     7861 ttcgctggag caatagcatg gtctaatgca aaggcgcagt actccgtaat actgaacaca
     7921 tctgccggtg aaaaccaagc tttgaccagt gaagacagct tatacattga gaaggtcatc
     7981 catgccttga atgcggcaat tgtgagcaga ggttgatgct tcgattagtt agtccgcacc
     8041 tatgtatttc gacataacac ctttctggtt ctactgcctc accgtagcgc ctctgattgg
     8101 tgctgtcgtt ttatttggtg tccacctcag tacattacat aagcatcgtt tggaatctca
     8161 aaaacttcaa ttggaaattc aaaatctgaa gaaagctcaa gaaaaatcag agagccgaat
     8221 attgatagct acaacagagg aaacaaaaga atacaatcag ccagcattca gcaggacagg
     8281 agcttatcaa gatgactatg ccaatttttc cgcaggtaat agtagcaagt cgagagcatc
     8341 caaaacgccc ctattagatt acggaataat ggtcat
//
LOCUS       DATMSK010000186        12261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_249855,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000186 DATMSK010000000
VERSION     DATMSK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 12261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12261
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249855"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VNZ84_06110"
     CDS             complement(<1..462)
                     /locus_tag="VNZ84_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66567.1"
                     /translation="MQSDNNVMSASQDTTTALDDQFGLAPQSQGLYDRRHEKDSCGVG
                     FVAHIKGQKSHDIVAKGLQLLDNLTHRGATGYDPKLGDGAGLLMQMPDTFMREEAAKL
                     GMTLPAVGQYAVGQAFLPQNADNRAKCEAIFAQIIAEENQTLLGWRDVPVDN"
     gene            complement(836..2116)
                     /gene="hemL"
                     /locus_tag="VNZ84_06115"
     CDS             complement(836..2116)
                     /gene="hemL"
                     /locus_tag="VNZ84_06115"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HWU66568.1"
                     /translation="MTSRNQQLFEKSQQLIPGGVNSPVRAFRSVGGTPIFFKKGQGSR
                     LWDADGKEYIDYINSWGPMILGHAHPEVIDAVQQAAANSLSFGAPTGLELEMAELINS
                     LVPSMEQVRLVSSGTEATMSAIRVARGFTKRSKIIKFEGCYHGHADALLVKAGSGLLT
                     FGEPSSAGVPPEVAAHTLTLDYNNTQMLKDTFASIGDEIACVILEPVVGNMNLIVPHQ
                     EFLQTLRELCTQHGTVLIFDEVMTGFRVALGGAQALYGITPDMTTLGKVIGGGLPVGA
                     FGGRKDIMSVLAPLGPVYQAGTLSGNPVAVTAGLTTLKLVQAPGFYEKLTAQTTKLMD
                     GFKQAAQAAGVAFSAQSVGGMFGLYFSAQCPRSYAEMMLADKNAFNKFFHSMLDSGIY
                     LGPSAFEAGFVSAAHTDADIAQTVQAATKAFAAL"
     gene            complement(2183..3199)
                     /locus_tag="VNZ84_06120"
     CDS             complement(2183..3199)
                     /locus_tag="VNZ84_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWU66569.1"
                     /translation="MRFLFYVCLLSTGLPLTYNVMAEPDHELVYRLKASVVKVHTITA
                     SGGHGVGSGVVVEKDTVATNCHVLANSSGISVSKFGDSISPVGMIADWQHDICLLKFA
                     YLELPAVKLAAAANLHYGDEVFSIGFPGGPPKPQTIAGKVRALYPYDGSVIVRTDAAF
                     VMGSSGSPTFNQDGELVSLSTFKSPGKHAYFYSVPVEWIQALMQQAPSPSTAPTASAF
                     WDVLPSSRPYWMQVVQPYQSGDWPALEEIARTWLVQEPSSAEACFYLASALHGQGKLS
                     EAQQAYERTTTLQPAHLDAWSGLAILAQQTQQPTLLSTAEQHVKALDIQAGTNLEQRL
                     QEAH"
     gene            3352..4092
                     /locus_tag="VNZ84_06125"
     CDS             3352..4092
                     /locus_tag="VNZ84_06125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWU66570.1"
                     /translation="MLITSEIVDLNTPTGVMRTYVHRPKHDGKVPAVLFYSEIFQQTG
                     PIERAARFLAGHGYAVLVPEVFHELNPIGTVLGYDDAGRDKGNADKMAKDVQAYDTDN
                     RAMIDYLNAQPWYDGNLGAAGFCIGGHLAFRAALQPEVKATACFYATDLHTTIIPNKP
                     GQHSMERVSEIKGELLMIWGKQDPHIPADKRAELYNRFAAIPGLHYTWHEFNGQHAFM
                     RDEGERYDAQLVNIGYQLTLQMFGNVLK"
     gene            4263..5777
                     /gene="gndA"
                     /locus_tag="VNZ84_06130"
     CDS             4263..5777
                     /gene="gndA"
                     /locus_tag="VNZ84_06130"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HWU66571.1"
                     /translation="MTTKNADIGLVGLAVMGQNLALNIADHGYTIAVYNRDPKKMLNF
                     IEECKKNEPSHERVVGHADLASFVLSIKRPRKIVLLVKAGSATDVTINALLPFLEQGD
                     IIIDGGNALWTDTIRREKELAAKGIEFIGSGVSGGETGARFGPSLMPSGTRKAWASLE
                     PIWRDIAAKVDPVTGAPLEGGAPGKPVQGGFSCAEYIGPDGAGHYVKMVHNGIEYIDM
                     QLICEAYWLMKNLLGMPADEIGKVFAEWNKGELSSFLIEITADILQQKDPSGKGFLVD
                     NILDTAGQKGTGQWTAANALELGAPANAIAAAVYARALSSLKEERVEASKILKGPTIV
                     QEKDKAAIIEAIKNALYCSKICAYAQGFQLIDKAQVAYNWKLNFGEIAQIWRGGCIIR
                     ARFLQKITDAYALNSRLKNLMLDPYFTNAMNEGQAGWRKVIALAVTNGIPAQGFAAAL
                     AYYDGYRSADLPANLLQGQRDFFGAHTYERKDQPRGQFFHLDWPEAGRPQLTIE"
     gene            complement(6014..6190)
                     /locus_tag="VNZ84_06135"
     CDS             complement(6014..6190)
                     /locus_tag="VNZ84_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66572.1"
                     /translation="MQIVESYQPASSQHLLVDEIYAPSLSTPAANKAAAQAQQHLKAI
                     TELHRFLESSCDCV"
     gene            6527..6730
                     /gene="thiS"
                     /locus_tag="VNZ84_06140"
     CDS             6527..6730
                     /gene="thiS"
                     /locus_tag="VNZ84_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWU66573.1"
                     /translation="MQIFVNGKAMSLPAGVINVLALVEHMQLTGKRIAIERNGDIVPR
                     SQFANVSLQSDDKLEIVGAVGGG"
     gene            6784..7566
                     /locus_tag="VNZ84_06145"
     CDS             6784..7566
                     /locus_tag="VNZ84_06145"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWU66574.1"
                     /translation="MNDALVIAGKSYQSRLLVGTGKYKDFTQTRAAIDASGAEIVTVA
                     IRRTNIGQNANEPSLLDYLPPSEFTYLPNTAGCYSAEDAVRTLRLARELLDDHRLVKL
                     EVLGDPNTLYPNVTETIAAAKTLVKEGFDVMVYTSDDPIIAKQLEDIGCMAVMPLASL
                     IGSGMGILNPWNLQIIIENAKIPVLVDAGVGCASDAAIAMELGCAGVLMNTAIAAARD
                     PVLMASAMKKAVEAGRESFLAGRMAKKLYSASPSSPTTGMIG"
     gene            7569..7952
                     /gene="apaG"
                     /locus_tag="VNZ84_06150"
     CDS             7569..7952
                     /gene="apaG"
                     /locus_tag="VNZ84_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HWU66575.1"
                     /translation="MAESDLYACEISVVTEYLAAQSSPEAGQYVYAYTITIRNTGKIS
                     SQLISRHWVITDALQQVREVKGLGVVGEQPLLAPGGEYSYTSSVMFSTPSGEMYGTYQ
                     MVAVDGHWFEVDIPMFWLHQAAGLH"
     gene            8039..9205
                     /locus_tag="VNZ84_06155"
     CDS             8039..9205
                     /locus_tag="VNZ84_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HWU66576.1"
                     /translation="MATVLWLAGCKSVPPATQSHPPSTEKPASVTPPVPAPAQPYALL
                     KAASWTDLPGLESEDWLASWSAWLQSCQGLKNKPDWQLVCATAQNVNKDAQAIQAYWQ
                     QYFNVYQTQQVDGAQQGLITGYYQPVLKGARHASARYPVPLYKAPSDLITVNLSALFP
                     ELKYKRVRGRLQGQSLIPYYTRSEIEQSQSPLAGNELLWVNDPVEAFFLQVQGSGIVE
                     LENGERLPVGYEDQNGHPYQSIGKLLVDRGEMVASEASMQGIKTWAAQHPEQLRGLLD
                     ANPSYVFFKLLPAGLSGPLGALGVPLTAARSLAVDPFYIPLGAPVYLASTYPNSQALL
                     QQLMMAQDTGGAIKGGVRADVYWGDGESAGKLAGAMRQQGQLWVWLPKTFPLPH"
     gene            9212..9787
                     /locus_tag="VNZ84_06160"
     CDS             9212..9787
                     /locus_tag="VNZ84_06160"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HWU66577.1"
                     /translation="MHRIQLSKDLRWQRHVVGQMALRPWLQERGSLTARLKRKYPDFS
                     VQVLSQGWRKSNVDEQSLLRLPAATRAWVREVMLMGQGQPQVFAHSVIARKDLRGTWH
                     GLRAIGRAPLGASLFADARVRRGRLHYRKLSAMHPLRLALGGHFPVSNRQPLWARRSL
                     FSLRHYRLLVTEVFLPPCQQQGLESASGMDF"
     gene            9787..10650
                     /gene="ubiA"
                     /locus_tag="VNZ84_06165"
     CDS             9787..10650
                     /gene="ubiA"
                     /locus_tag="VNZ84_06165"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HWU66578.1"
                     /translation="MQSIAKWHAYYRLTRLDKPIGIWLLLWPTLWGLLIAGHGFPQPW
                     VVVVFVVGTVLMRSAGCALNDIADRHWDGAVARTKLRPLATGEVSVKEAYGVALGLSI
                     MAFLLVCTLGWHVVLWSVPALFLAVSYPYTKRFFAIPQAYLGIAFGFGIPMSFVALQG
                     EVSAVAWWLLLANVFWSVAYDTAYAMVDRADDLKIGIRSSAITFGRFDVLAIMICFGA
                     MMLILAYVADEIALKWPFWLGWLVAAMQLLRQYALIRDRQPRECFKAFLENFWIGAAL
                     TSGMAAAYWLG"
     gene            complement(10642..>12261)
                     /locus_tag="VNZ84_06170"
     CDS             complement(10642..>12261)
                     /locus_tag="VNZ84_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66579.1"
                     /translation="IHKVKKQHHFFAANHRLRLLVFLFIAFTPLSQASELTTVNIRSA
                     PGEPLLAQVAINNILPDTDVGCFSLKQAPTDATPVRLEYTTLGNQQGELTLWSENATS
                     RTLRLTLIDQCQHTQQTLTIQTDNTVTITSPSASPPKNTSEPATDVMVRVIEPAHPAS
                     SPAIPVEAYTALQAQINDLQANFKQLQIKSDVLYASNLAQANILATLKTQLTQLQHEN
                     HLLRTLVIALGLALIASGFFFADWLRRNAAAPKVVKSRQSSRTSDATATGDHASGPST
                     QPGAGKAFSSTLPPGNRHTANPATQPPAQTKPASRLKSVPMHANDDAVAPYLVPQKLS
                     SLQSTQTVMQDASRLFTHGRVNQAIDHLQQHLAKHPKCSPWMWLYLLDLLSKEGKQQE
                     FDIVAGECRKHFNLNVEHTPDLKQQGIESFPRVMKALQQAWGTPQVIALIDDLVYNTR
                     LVPRMGFERSVFEDLMLLKEIACQTQNLPAVPPVPKSDSHEEPISGNLRELQQLSEDI
                     YILAPAEHTPFQYWTDFTFELDEPSTLRRSA"
BASE COUNT         2799 a         2799 c         3449 g         3214 t
ORIGIN      
        1 gttgtcgact ggcacatcac gccagcccag taatgtctgg ttttcttcag caatgatctg
       61 ggcaaaaatg gcttcgcatt tggcacggtt gtctgcgttt tgcggcaaga atgcctggcc
      121 gacggcatat tggccgacag ctggcagggt catgcctagt ttggcagcct cttcacgcat
      181 aaaggtgtca ggcatttgca tcagcaagcc ggcgccatcg cccagtttcg ggtcgtaacc
      241 agtcgcacca cggtgcgtca ggttgtccag cagttgcagg cctttggcaa cgatatcatg
      301 gcttttctgg cctttaatat gggcgacaaa gcccacgcca cagctatctt tctcgtggcg
      361 tctgtcgtat aaaccctgcg attgcggagc taaaccaaac tggtcgtcca gagcggttgt
      421 ggtatcttga gaagcactca tcacgttgtt gtcagactgc atgtccattg ccagattctt
      481 cacttacaaa acatttggag gaaccttcaa tgataccgtt aaagtttagc aatttcaatt
      541 gtttagcctg cgttaaataa cggataaatc tttttttgca tttcaattga gaatggtttt
      601 tatttaccag ttgatctggc ttctgtaagg gttttcttgc tggctgagat tgcttggcgg
      661 ttgttcttta agtgtgcaag cggtcaagaa tgcatgcttg taatgtcgga aaagtttgct
      721 ctttaatgct aggtgagagt gattctaatg aagaacggtc ttaatgagcc gtgggaatgg
      781 cgcgtatggg ttgcctgcca gcgcgatagt gagcgttggc aggctttcaa ttcatttaaa
      841 gtgcagcaaa tgctttagta gcggcttgca cggtttgtgc aatgtccgca tcagtatgcg
      901 ctgccgagac aaagcctgct tcaaacgcag atgggccaag gtagatgccg ctatccagca
      961 tgctatggaa aaatttattg aatgcatttt tatctgccag catcatttca gcgtaactcc
     1021 ttgggcattg ggcgctgaag tacaggccga acataccgcc tacactttgt gcgctgaacg
     1081 cgacaccggc agcttgtgct gcctgcttga aaccatccat gagttttgtg gtttgcgctg
     1141 ttagtttttc atagaagcct ggtgcttgaa ccaatttcaa ggtcgtgagc cccgcggtga
     1201 ccgccaccgg gttgcccgac aatgtacccg cctggtaaac cgggcctagt ggagccagga
     1261 cgctcataat gtccttgcgg ccaccaaagg caccgactgg taatccgccg ccgatgactt
     1321 tgcccagagt ggtcatgtct ggtgtaatgc catataaggc ttgtgccccg cccaaggcta
     1381 cgcggaagcc tgtcatgact tcgtcaaaaa tcaagacggt gccatgttgg gtgcacagtt
     1441 cacgcagggt ttgcaagaac tcttgatgcg gcacaatgag gttcatattg ccgacgactg
     1501 gttcaaggat cacgcacgcg atttcatcgc cgatgctggc aaaagtatct ttgagcatct
     1561 gtgtgttgtt gtagtccagc gtcaaggtgt gtgcagccac ttctggtgga actcccgctg
     1621 agcttggttc gccaaacgtg agcaggccgg aaccggcctt taccagcagc gcgtcagcat
     1681 ggccatggta acagccttca aatttgataa ttttgctgcg tttggtaaag ccgcgagcga
     1741 cacggattgc gctcatcgtg gcttcggtac cgctactcac caggcgtacc tgctccatgc
     1801 tgggcaccag gctgttgatc agttctgcca tttccagttc aagaccggtt ggtgcgccaa
     1861 agctcaggct attggcagcc gcttgttgca ccgcgtcaat cacttcaggg tgcgcatgcc
     1921 ccaggatcat cggcccccag gagttgatgt aatcaatata ttctttgccg tccgcatccc
     1981 ataatctgga tccttgcccc tttttgaaga agataggggt gccacctacg ctacgaaacg
     2041 cacgtaccgg cgagttgaca ccgcctggga tcagttgctg ggatttttca aaaagttgct
     2101 ggttgcgaga ggtcataagt gtgcaatctg ggtaacatca aaaaagcata ttctatcaga
     2161 ggtggggctg cagttgccca tgctagtgag cctcttgcag gcgttgttcg aggttggttc
     2221 cggcttggat gtccagtgct ttgacatgtt gctcggcggt gctgagtaag gttggctgtt
     2281 gtgtttgctg tgccaggatg gcgagtccgc tccatgcgtc tagatgcgct ggctgcaagg
     2341 tggtggtgcg ctcataggcc tgctgcgcct cagagagttt gccttggccg tgcagggcgc
     2401 ttgctaggta aaaacaagct tcagccgagc tgggttcttg caccaaccat gtccgcgcga
     2461 tctcttcgag tgctggccag tcgccacttt ggtaaggctg gacgacttgc atccagtagg
     2521 ggcgtgacga tgggagcaca tcccagaacg ccgacgcggt gggcgcagtt gaaggggaag
     2581 gtgcttgttg catcaaagcc tgtatccact ccacaggcac gctataaaaa tatgcatgtt
     2641 tgccagggct tttgaaggtg ctgagcgaca ccagttcacc atcctggtta aaggtagggc
     2701 tgccactcga ccccatcaca aacgcagcgt ctgtgcgtac aatgacgctg ccatcgtaag
     2761 gatataaggc acgtaccttg ccagcgatgg tttgtggttt gggtgggccg cctgggaagc
     2821 caatggagaa cacctcatcg ccataatgca ggttcgcggc tgcggctaac tttactgctg
     2881 gcagttcgag gtaggcaaat ttgagcaggc agatatcgtg ctgccagtcg gcaatcattc
     2941 ccaccggact gatgctgtcg ccaaatttgg aaacgctgat tccactactg tttgctaata
     3001 cgtggcaatt agtcgcaaca gtgtcttttt ccactacaac accgctacca acgccgtgac
     3061 ctccgcttgc agtgatggtg tgtactttaa ccacggaagc tttaaggcga taaactagct
     3121 catgatcagg ctcggccatt acgttgtatg tgagtggtaa gcctgtagaa agcaagcaga
     3181 cataaaataa gaagcgcata tggactcccg tgatcaggcg aactttaagg atcgtgatta
     3241 gtttttgacc tgcaaagtgc aaaaaattct cattcagggt cagattaccc gtgaaatgct
     3301 atgcaaacag cgctagaata gccgatattt ttgattgtct aaggatttac catgctgatt
     3361 acaagcgaaa tcgttgattt aaacactcca accggggtga tgcgcaccta cgtacatcgt
     3421 ccaaaacacg atggcaaagt gcctgcggtt ttgttttatt ctgaaatttt ccagcaaact
     3481 ggcccgattg agcgcgcagc ccgttttctg gctggtcatg gctatgccgt gctggtgccg
     3541 gaagtgttcc acgaattaaa cccgattggg actgtgctgg gttatgacga cgctggccgc
     3601 gataaaggta acgccgacaa aatggctaaa gatgtgcaag cctatgacac tgataaccgt
     3661 gccatgattg attacctgaa tgcgcagccg tggtatgacg gcaacctggg tgcagcgggt
     3721 ttctgtattg gcggtcatct ggcgttccgt gctgccttgc agccagaagt gaaggcaact
     3781 gcctgcttct atgccaccga tttgcacacc actattattc caaataagcc aggtcaacac
     3841 agcatggagc gtgtgagtga gattaaaggc gagttgctca tgatttgggg caagcaggat
     3901 ccacacatcc cagccgacaa gcgtgcagag ctgtataacc gctttgcagc gattcccggt
     3961 ttgcattaca cctggcatga atttaatggg caacatgcct ttatgcgcga tgaaggtgaa
     4021 cgttacgatg cgcagttggt caacatcgga tatcaattga ccttgcaaat gttcggtaac
     4081 gtactcaagt aagcgaagac acaatcactt gaggtaaaga aaactatggt gctgaatgtt
     4141 ttatgcctgt tgcgattcat taaacaacac gcatggaatg ttcaatacat accctagtcc
     4201 aagcacaggc caaaagcctg tgcttttttt tcaactaaat taaccattta ttagagataa
     4261 ctatgactac aaaaaatgcg gatattggat tggtaggatt ggcagtgatg ggccaaaacc
     4321 tggcgcttaa cattgcggat catggctata ccatcgcggt ctataaccgt gacccgaaaa
     4381 aaatgctgaa cttcatcgag gagtgcaaaa agaatgagcc ttcacatgag cgtgtagttg
     4441 gccatgcgga tctggcttct tttgtattaa gcatcaaacg tcctcgtaaa atcgtgttgc
     4501 tggtgaaggc cggtagcgcg acagatgtga cgatcaacgc tttgctgcct ttcctggagc
     4561 agggcgacat tattatcgat ggcggcaatg ccttgtggac ggataccatc cgccgtgaaa
     4621 aagagctggc tgccaaaggt atcgagttta ttggctctgg tgtttccggt ggcgaaaccg
     4681 gtgcacgttt tggcccctcc ctgatgccat cgggaacacg taaagcctgg gctagcctgg
     4741 agccaatctg gcgtgatatt gccgccaagg ttgaccctgt cactggcgca cctttagaag
     4801 gtggtgcacc gggcaagccg gttcaaggtg gtttctcttg tgccgagtat attggcccgg
     4861 acggtgctgg ccattatgtg aaaatggtac acaacggtat tgaatacatt gatatgcagt
     4921 tgatttgtga agcctattgg ctgatgaaaa acctgcttgg catgcccgcc gatgaaatcg
     4981 gtaaagtctt tgctgagtgg aacaaaggtg agctatccag cttcctgatt gagattacgg
     5041 cagatatcct gcaacaaaag gatccgtctg gtaagggttt cttggtagat aacattctgg
     5101 atacggccgg tcaaaaaggc actggccaat ggactgcagc taacgcgctg gagttgggtg
     5161 caccagccaa tgcgatcgcg gcagccgttt atgcgcgcgc gctatccagc ctgaaggaag
     5221 agcgagtaga agccagcaaa atcttgaaag gcccgacgat tgtgcaggaa aaagataagg
     5281 ctgcgattat tgaggccatc aaaaatgcgc tgtattgctc caaaatttgc gcttatgcac
     5341 aaggcttcca gctgatcgac aaagcacaag tggcttacaa ttggaaactc aactttggtg
     5401 agatcgcgca gatctggcgt ggggggtgta ttatccgtgc gcgcttcctg caaaaaatca
     5461 ctgatgctta tgcattaaat tcacgtctga aaaacctgat gctggatcca tacttcacca
     5521 atgcgatgaa tgaaggccag gctggctggc ggaaagtcat tgcgttagca gtgaccaatg
     5581 gtataccggc gcaaggtttt gctgcggcat tggcttacta cgatggttac agaagcgctg
     5641 atttgccagc taacttgttg caaggtcaac gcgacttctt cggcgcgcat acttatgagc
     5701 gcaaggacca accacgtgga caattcttcc atttggactg gcctgaggct ggtcgtccgc
     5761 aattgacgat tgagtaattg ctctgctggg tgccggtaac ggcacttgga cataaaaaag
     5821 atggcgctag tcgccatctt tttttattga ttcatgtttt cgaaaaacaa aattgaaagg
     5881 cccaagtttt caggggaaac cagggctgat acaggctgaa tgatgttcag tgtataaaca
     5941 ttaacgcaga tctctgcgct aactaaaaaa tcgtgacccg ctatcgggtc ttgcgccagc
     6001 taatcctgcg tgattaaacg cagtcgcagc tggattccag aaagcggtgt aactcggtga
     6061 tggctttcag gtgttgttgc gcttgggcgg ctgctttgtt ggcggcggga gtgctcaagc
     6121 tgggggcgta aatttcatcc actaaaagat gctgggacga agcgggttgg tatgactcta
     6181 caatctgcat ttcaatcttc cttttgggat ttccaatatt tcggctacaa gtcgacgaat
     6241 tgtgttcgtc gtgcatgtgg cgccaaaatt aacaaaaagt tccttgcgca gcaatgcgtt
     6301 atctgtcgta atgactaaag ttgtcaattt gttgccagat atttgcatgg ctacaacata
     6361 caagcaaaca tggtgccatg tttgctctta acaacgcatg ttcattccat tcggatgaca
     6421 aggctcgaat gacacatcaa ataaagacca tgcacgaaaa acagaaagca aaatgcgctg
     6481 atttttcgta aaatgtcggt ttgattaatt aaaaatgaag agcagtatgc aaatttttgt
     6541 caacggcaaa gccatgtcgc taccagcagg cgttattaat gtgcttgcat tggttgagca
     6601 tatgcagctg acgggtaagc gtattgcgat tgaacgtaat ggcgatatcg taccgcgcag
     6661 ccagtttgct aatgtgtcat tgcagtcaga cgacaagctg gaaattgtag gcgctgtcgg
     6721 tggtggttaa ttgcagcctg gctgcgatat tgacagaaca gaattgaata gagagttgtg
     6781 agtatgaatg acgctttggt aatcgccggt aagtcttatc agtcccgcct attggtgggg
     6841 accggcaaat acaaagactt tacgcagacg cgtgccgcaa ttgatgccag cggtgccgag
     6901 attgttacgg tggcgattcg ccggaccaat attggtcaga atgcaaacga accttctttg
     6961 ctagattatt taccccccag tgaatttacc tatttgccaa atacggctgg ttgctacagt
     7021 gcggaggacg ccgtgcgtac tttgcgtctg gcgcgcgagc tactggacga ccataggctg
     7081 gtcaagctgg aagtgctggg tgacccaaac actttgtacc ccaatgtgac agaaacgatt
     7141 gcagcagcta aaactttggt caaagagggc tttgacgtga tggtgtatac ctctgatgac
     7201 ccgattattg ccaagcagtt ggaagatatt ggctgtatgg cggtgatgcc gttggcgtca
     7261 ttgattggct caggcatggg cattttaaac ccatggaacc tgcagatcat tatcgaaaat
     7321 gccaagattc ccgtgctggt ggatgcaggt gtgggctgcg catctgatgc cgcgattgcc
     7381 atggagttgg gctgcgccgg tgtgctgatg aatactgcca ttgccgctgc acgcgatcct
     7441 gtgctcatgg ccagtgccat gaagaaagca gttgaggctg ggcgtgagtc attcctggcg
     7501 ggacggatgg cgaaaaagct gtattctgcc agtcccagtt caccgacgac agggatgata
     7561 ggctaatcgt ggcagagagt gatttatatg cttgtgaaat ctcggtggtc accgagtatc
     7621 tggctgcgca atcttcacca gaagccgggc agtatgtgta tgcctacacg attaccatcc
     7681 gcaatacagg caaaatctct tcgcaactga tcagccgcca ttgggtcatt acagatgcct
     7741 tgcagcaggt gcgagaggtc aaaggcttgg gtgtggtagg cgaacagccg ctgctggcac
     7801 cgggtgggga gtacagttat accagtagtg tcatgtttag cacaccaagt ggtgaaatgt
     7861 atggcactta tcaaatggtt gcggtagatg ggcactggtt cgaagtcgat attcccatgt
     7921 tctggctgca tcaggcggca ggtctgcatt aatcggcctg cgaaagtaat ggttttaagt
     7981 gaattaacga aaagtgccgg ttatcaacaa tctttttaac gtccgtctgg gtttattaat
     8041 ggcaactgta ttgtggttgg caggctgtaa aagtgtgccg ccagcaacac agtcgcatcc
     8101 gccatcgaca gagaagccag cttccgtaac gcctcctgta ccagcccccg cgcagcctta
     8161 tgcgttgctg aaggctgcga gttggaccga tttgcccggc ttggagagcg aagactggtt
     8221 ggcgagctgg agtgcttggt tgcaaagctg tcagggacta aaaaataagc ccgattggca
     8281 attggtctgt gctacggcgc aaaacgtgaa taaagatgct caggcgatac aagcctactg
     8341 gcagcaatat tttaatgtgt accagacgca acaagtagat ggtgcgcagc aaggattgat
     8401 cacagggtat taccaacccg tgttgaaagg tgcccgtcat gccagcgccc gctatccggt
     8461 gcctttatac aaagcaccat cagacctgat cacagtgaat ttgtctgcgc tgtttcctga
     8521 acttaaatac aagcgagtac gtggtcgctt gcaggggcag tcgctgatcc cttattacac
     8581 gcgctcagag attgagcaga gtcagtcgcc actggctggt aacgaattgt tatgggtgaa
     8641 tgatcctgtg gaggcttttt tcttgcaggt gcaggggtcg gggattgttg agctggaaaa
     8701 tggcgagcgt ttaccggtcg gctatgaaga ccagaacggg cacccttatc aatcaatagg
     8761 taagctgttg gttgatcgcg gagagatggt ggcaagtgag gcttcgatgc aggggattaa
     8821 aacctgggct gcgcaacatc ctgagcaatt gcgcggcttg ctggatgcga atcctagtta
     8881 cgtgtttttt aagctgttgc cagcaggctt gtccgggcct ttgggcgcac tgggcgtgcc
     8941 attaacggct gcccgcagtc ttgcagtgga tccattttat attccattgg gtgcgccagt
     9001 ttatttggcg agcacctatc caaactcaca agcgctttta cagcaactaa tgatggcgca
     9061 agatacaggt ggcgcgatta aaggtggtgt gcgcgcagac gtttattggg gcgatggcga
     9121 atcggccggt aagctggcag gcgcgatgcg acagcaagga cagttgtggg tatggttgcc
     9181 caaaaccttc ccgctgccgc attaggcttt tatgcacagg attcaattga gcaaagacct
     9241 tagatggcag cgtcacgttg tcgggcagat ggccctgcgc ccctggttac aggagcgtgg
     9301 ctctttgacg gcgcggctca agcgtaagta ccctgatttt tcagtccaag tcctttccca
     9361 aggatggcgt aagtcgaatg ttgacgaaca atcattacta cggctgccag ccgccacccg
     9421 tgcctgggtg agagaggtga tgctgatggg ccaaggacag ccgcaggtgt ttgcacatag
     9481 cgtcatcgcc cgtaaggatt tacgtggcac ctggcatgga ttgcgtgcta ttggccgtgc
     9541 tccgttgggc gcctcgctgt ttgctgacgc acgagtcagg cgtggccgct tgcattaccg
     9601 caagttatcc gcgatgcatc cgcttcgcct cgctctaggt gggcatttcc cggtttccaa
     9661 tcgacagcca ttgtgggcca ggcggtcact atttagtttg cgacattatc gtttgctagt
     9721 gacggaggtt tttttaccgc cgtgtcagca gcagggcctt gaatcagcct cgggaatgga
     9781 cttttaatgc aatcaatcgc taaatggcat gcgtattacc ggctgacccg gctagataaa
     9841 ccgataggga tctggctgct attgtggcct actttgtggg gcctgttgat tgccggacat
     9901 ggattcccgc aaccttgggt cgtggtggtt tttgtagtgg gaacggtatt gatgcgttcg
     9961 gcgggttgtg cgctgaatga cattgctgac cggcactggg atggtgctgt tgcgcggaca
    10021 aaactacggc cgctggcaac gggcgaagtt tcggtgaaag aggcgtatgg tgtggccttg
    10081 ggtctttcaa tcatggcgtt tttgctggtg tgtacgcttg gctggcatgt ggtgctatgg
    10141 tctgtgccgg ctttgtttct ggcggtgagc tatccttata ccaaacgatt ctttgcgatt
    10201 ccgcaagcct atctcggtat tgcgtttggg tttggtatcc cgatgtcttt tgtggccttg
    10261 caaggagagg tgtcggcggt ggcatggtgg ctactgctgg ccaatgtatt ctggtccgta
    10321 gcttatgata cggcatatgc catggtagac cgtgcagatg acctcaagat tggtattcgc
    10381 tcttcagcca tcacgtttgg acgttttgat gtgctggcga ttatgatttg ttttggggca
    10441 atgatgctca tacttgctta tgtggcggat gagattgcgc tcaagtggcc gttttggttg
    10501 ggttggcttg tggctgcgat gcaactgttg cgacagtatg cgcttattcg agatcgccag
    10561 ccccgagagt gtttcaaagc gtttctggaa aatttctgga ttggggccgc gctgacgtcg
    10621 gggatggctg ctgcttattg gttaggctga ccttcgtaag gtcgagggtt cgtccagttc
    10681 gaatgtgaaa tccgtccagt actggaaagg cgtatgttca gcaggcgcca aaatgtagat
    10741 gtcttcagac aactgctgaa gttcgcgcag gttgccactg atcggttctt cgtggctatc
    10801 gctcttgggc acaggcggta ccgccggcag gttttgtgtc tggcaggcaa tttcttttag
    10861 cagcatcaag tcttcaaaca cgctgcgttc aaagcccata cggggcacca agcgggtgtt
    10921 gtaaaccaag tcgtcaatca aggcaatcac ttgtggcgtt ccccaggctt gttgcagtgc
    10981 tttcatcacg cgagggaatg actcaatccc ttgttgtttc aggtctggtg tgtgctccac
    11041 attcaggttg aaatgcttgc ggcattctcc tgccacaata tcaaactctt gttgtttgcc
    11101 ttctttggat agcaggtcca gtaaatacag ccacatccat ggggaacatt tgggatgttt
    11161 ggccaaatgt tgctgcaagt gatcaatcgc ttggttcacg cggccatgcg taaacaagcg
    11221 ggaggcatcc tgcatgacgg tttgtgtgct ttggagtgaa ctaagttttt gcgggaccag
    11281 gtatggggct acggcatcat cgttggcatg catcggtacc gactttagcc ggctggcagg
    11341 tttggtctgc gcgggtggct gtgttgccgg gttggctgta tgccggtttc cgggaggtag
    11401 tgtagaggaa aatgctttgc ctgcgcctgg ttgtgtcgaa ggcccggagg catgatcacc
    11461 cgtcgcagtg gcgtcgctcg tgcgtgagga ttgtcggctt ttgactactt tgggggccgc
    11521 agcattccgg cgtaaccagt cagcaaagaa aaagccactg gcaatcaggg ctaatcccag
    11581 tgcgatgacc agggtgcgca gtaaatggtt ttcgtgttgc agttgcgtga gctgggtttt
    11641 cagggttgcc aatatgttgg cttgtgccag gttgcttgcg tacagtacat cagatttgat
    11701 ttgtaattgc ttgaaattcg cttgcaaatc attaatttgt gcctgcaggg ctgtgtaagc
    11761 ctctacaggg attgctggtg acgacgctgg gtgagcaggc tcaatgactc tcaccatgac
    11821 atccgttgct ggttcggaag tgtttttagg tggtgatgct gatggtgaag tgattgtgac
    11881 agtgttatct gtttgtatgg tcaacgtttg ctgcgtgtgc tggcattgat caattaacgt
    11941 taatctcagt gtcctgctag tagcattttc agaccagagc gttaactcac cttgctgatt
    12001 accgagcgtg gtgtactcta atcgcactgg tgttgcatcc gtcggtgctt gtttcaagct
    12061 gaagcagccg acatcggtgt ctgggagaat attgttaatg gctacttgtg ccagtaaagg
    12121 ctcgccagga gcagaccgaa tgttgacagt ggtcagctcc gacgcttgag aaagcggagt
    12181 aaatgcgatg aataggaaaa ccaatagccg cagccgatgg ttggctgcaa aaaaatgatg
    12241 ctgtttttta accttgtgta t
//
LOCUS       DATMSK010000187         8309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_62192,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000187 DATMSK010000000
VERSION     DATMSK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62192"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..624
                     /locus_tag="VNZ84_06175"
     CDS             406..624
                     /locus_tag="VNZ84_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HWU66580.1"
                     /translation="MKSVRKSLAWSLLLVLALSTTACGSMSTRGKSTALGAGIGAVGG
                     AVLTGGSALGTVGGAAVGGVIGNQIDKK"
     gene            719..1825
                     /gene="hemH"
                     /locus_tag="VNZ84_06180"
     CDS             719..1825
                     /gene="hemH"
                     /locus_tag="VNZ84_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWU66581.1"
                     /translation="MSYYQPEPAYTHGSQPKVGILLANLGTPDAPTAKALRPYLQQFL
                     MDRRVVEIPRFIWCWILHCIILVVRPKKSAEKYASIWGKDGSPLMAYAQQQKKLLQGY
                     LTQKINSPFAVELGMTYGNPSMKSAIKSLKAQGCDRILVFPLYPQYAASSTAAALDAV
                     WRVLLKMRNVPAIRTIKHYHDHPLYIQALATHIQTYWKQHGKPEKLVMSFHGVPKFHL
                     LKGDPYHCECHKTARLLAEALGLNKEQYEVAFQSRFGRQEWLQPYLANTLDALGKQQL
                     QRIDVVCPGFSSDCLETLEEIAMEGKHLFQSVGGGDYHYIPALNSEAVWIEAMRDIAL
                     ENLQGWVSNAFDQTKANAEAQTSKQLALAMGADR"
     gene            1933..2922
                     /gene="rfaD"
                     /locus_tag="VNZ84_06185"
     CDS             1933..2922
                     /gene="rfaD"
                     /locus_tag="VNZ84_06185"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HWU66582.1"
                     /translation="MIIITGGAGMIGSIMAWHLNNKLGRQDLVIVDRITHENQWQNLV
                     HRHYAEYLDKDQLFEFLEDNDGITAVVHMGAISATTERDFNKLVADNMHYSQDLWSWC
                     TEYEVPFFYASSAATYGGGEQGYDDASIENLRPLNGYGYSKHFFDQWVLQQVAQKQAT
                     PPAWAGFKFFNVYGPNEYHKERMASVAYHTFNQFSETGTMRLFKGTKAGVEDGMQLRD
                     FVYVKDVADVMGFFLEGALKNKPVTSAIYNIGTGQARSFKDLATNVMTSMSREPNITY
                     IDMPQDLQGKYQYFTQAEMKKLRDAGYKKPFTTLEEGVKDYVQNYLLQDDPYA"
     gene            2946..3506
                     /locus_tag="VNZ84_06190"
     CDS             2946..3506
                     /locus_tag="VNZ84_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HWU66583.1"
                     /translation="MNHIEYLKGEHAAHMAMFQALEPLFPLIGDVGNLLRDCIQRGGK
                     VLLCGNGGSAADSQHIAAEIVGRFKKERKGLPSIALTTDTSILTSVGNDYGYDYIFAR
                     QVEALCRPEDILIGLTTSGNSANVVRAIETANEIGATTIGLTGGSGGKMNALCTHNIV
                     VPSNVTARIQEAHIFIGHCLCEILES"
     gene            3506..4978
                     /gene="hldE"
                     /locus_tag="VNZ84_06195"
     CDS             3506..4978
                     /gene="hldE"
                     /locus_tag="VNZ84_06195"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HWU66584.1"
                     /translation="MTGNALHSDLATVVAHFGEQTQHALVIGDVMLDRYLMGEVNRIS
                     PEAPVPVVLIKSEQQRAGGAANVAANLALLGVTTHMIGLIGNDHEGQRLLAAMSEYGI
                     NTQAMITTPARPTIAKTRILGGHQQMMRLDQEVTGALSDKEAAQVMQATQTALQQHPA
                     LIILSDYAKGLLTEQVCQQVIDYGRQHNIPVLVDPKGRNYDKYRGATALTPNKKETAE
                     ACDTQMSDAALIDKACTLKTSLSLDFLAVTRGEEGITLIADQTTHLSATAKQVFDVSG
                     AGDTVIATLAAGLMHGLSSLDSLALANHAAGVVVGKVGTVPITKADLLDAIATAQGSQ
                     QAHKISELPDLLQKVANWKQQGQKIVFTNGCFDLLHAGHVTYLEGAKKHGDRLILGLN
                     TDRSVSALKGPTRPVVNEKDRARVLAALESVDAVILFDEDTPLNLINAIQPNIIAKGS
                     DYTADQVVGGKEVLSWGGEIALIDLVEGRSTTNLIKKMHA"
     gene            4975..5997
                     /gene="waaF"
                     /locus_tag="VNZ84_06200"
     CDS             4975..5997
                     /gene="waaF"
                     /locus_tag="VNZ84_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HWU66585.1"
                     /translation="MNKILVMGPSWVGDMVLAQSLFKQLKIEQTDCQITVAAPAWTLP
                     LLERMPEVSQAIALPFKHGDLALRERFKLGRELAKEGFTQAILLTNSLKSAILPWAAR
                     IPVRTGFKGEMRYGLVNDMRPLDKSTLKQTVQRFVALGLPRHATLPESLPQPTLLANA
                     ANAPSLLRKFDLPTHGTVLGLCPGAEYGEAKRWPTEYYAEVANTALNQGWQVWLFGSE
                     KDQPVTTSIQQQTGQRCVDLAGKTKLGEAIDLMSLCHTVISNDSGLMHVAAALDKKLI
                     AIYGSSDPKHTPPMHPQAVIEYLGLTCSPCFERTCPLGHLNCLRQLKPASVLAKLPWK
                     ILETGK"
     gene            5994..7535
                     /locus_tag="VNZ84_06205"
     CDS             5994..7535
                     /locus_tag="VNZ84_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66586.1"
                     /translation="MTSFSFLRKISQSQWLNIYLLAAFLIACKIMYNQHGWVNNDSLL
                     YFEQARLIAAGQLQQAFGLFTWILYPGLLAIIHKLTGLSIHVSAISLNVLCFVIFVAG
                     FQRLLIEAGAKLRTLHWGMLLLFSTQYIVGDVLGMLLRDEGFWAAYTWGLLFWLRALK
                     SQHWKHILQFQAVMAVAVLFRIESSAYLLALPLCALLLPQLTWLQRLKLWLKANSLAL
                     LTTVIVAIGLLAGVLQQEQLGRLQEVFTQIAHLFTERITFINQKAAILGHQVLGEDLD
                     QYGTFSLWSSLVLIAIFKTLKVAGLPALAVILWPRQTWWSKLPEVTRYLVISTLTISF
                     VVSLVIIFNVFILSSRYVIASGIVMLLLAAFAITEWQQRWPKWVSKSYVLLLAVLFMY
                     SLFDKHEIDLDRVAVDYIAQINPQHKPAFYDTENARFYADQPYTDRILGRVAFPQLVN
                     KNEIDQYDYFMITISKDPADIAYERQALQVLGEHQYKLVNTLYGWRNKTKALIFVRHP
                     ENKPN"
     gene            7586..>8309
                     /locus_tag="VNZ84_06210"
     CDS             7586..>8309
                     /locus_tag="VNZ84_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66587.1"
                     /translation="MTPLPLNLALTVPANLRASFKQLLRFSLLWVVLFACTANVCNSF
                     FIKWGFRDDSHYASLLSVIDGSAYQPYVYRSTVPRLADLAIQHIPAEKQEQLFHRIIS
                     SDKLKHHFFNDVPLEKWTPRIALDYHLMYGLTLLSFLITLVYLRKIFLYFSENYSASI
                     LAVVLFSLMYPLTFKRGGYFYDFFELLGITVSTYYFLTNRKLLASILLMLTAFNKETA
                     FVAAFGFFFLHAKDYPMQKRLAY"
BASE COUNT         2175 a         2110 c         1971 g         2053 t
ORIGIN      
        1 tacaggttgt aggcagcagt gtgggtgcca ccgcaggtta caatggaacg atcattacta
       61 ccgctgtaac agagcctgaa agtttcgccc taatgcttgc cgggattagt attattgggg
      121 caatcggacg cagaaagctg cgcaagcaat tgccaagtct tagttattaa tcaggtaagt
      181 taataaccct tcaaatctcc gcctcatgcg gggatttgta gtgcgtaaac atcttaacaa
      241 gttacgtaaa cattagactc gaccatcctc gcctcaataa agcattacat tgcctgctaa
      301 agcttgtccg aattatctga caccacaatc agcattcatc ctctactcgc ttaactgcat
      361 tattttcata atccacagca catattaccc attaggagag atgaaatgaa aagcgtacgc
      421 aagtctttag cctggtcact attattagtt ctcgcactta gcacaacagc ctgtggctca
      481 atgagcactc gcggtaaaag tacagcttta ggcgctggca ttggtgcagt aggtggtgca
      541 gtgttaactg gtggcagtgc cttgggtact gtcggcggcg ctgctgttgg tggtgtgatc
      601 ggcaaccaga tcgacaaaaa ataacgtcaa acaggcaact gatgagccaa gcttagcggt
      661 tgccgcactt ttttagttgc tgtgcgtcat aagggcattt atcgcttaaa atgcccttat
      721 gagctactac caaccagaac ctgcttacac ccacggcagc caaccaaaag tgggcatctt
      781 gctagccaac ctcggtacac ctgatgcccc gacggcgaaa gcactccgtc cttatttaca
      841 gcaattcctg atggatagac gcgtggtaga aatcccccgc tttatctggt gctggattct
      901 gcattgcatc attctcgtcg tgcgtcctaa aaaatcagcc gaaaagtatg ccagcatctg
      961 ggggaaagat ggttcaccgc tgatggcgta tgctcagcaa caaaagaaac ttctgcaagg
     1021 ctacctgaca caaaaaatca actcaccatt tgccgtcgaa ttaggcatga cttatggcaa
     1081 tcccagtatg aaaagcgcga taaagtcgct taaagcccag ggctgtgacc gcattctggt
     1141 gttccccctc tatccgcaat acgctgctag cagtaccgct gcagctttgg acgccgtgtg
     1201 gcgcgtatta ctcaaaatgc gtaacgtgcc tgctatccgc acgatcaagc actaccatga
     1261 tcatccgctg tatattcagg ccttggccac ccatatccaa acttactgga aacaacatgg
     1321 caaacctgaa aaactggtca tgagttttca tggcgtacca aaattccacc tgctaaaagg
     1381 cgatccctac cattgcgagt gccataaaac ggcacgcttg ctagcagaag ctttaggctt
     1441 aaataaagag caatacgaag tagcgttcca atcccgcttt ggccgccagg aatggctaca
     1501 accctacctc gccaacacgc tggatgcact aggcaaacaa caactccaac gcatagacgt
     1561 cgtttgccct ggctttagca gtgactgcct ggaaaccctc gaagaaattg ccatggaagg
     1621 caaacacttg ttccagtccg ttggcggtgg tgactatcac tacatccctg cgctgaatag
     1681 cgaagcggtg tggatagagg ccatgcggga tattgcatta gaaaacttac agggttgggt
     1741 aagtaatgcg tttgaccaga caaaagcgaa tgctgaagca caaaccagca aacaactggc
     1801 attagcaatg ggcgcagatc gctaacttag tctagagggg atgagttgcg aaagaacaga
     1861 cgcatccatt ccactgccag ccttgcttga ttacgcgcta taatcgcgct ttaacgtttt
     1921 tatgactccc ctatgatcat tatcaccggt ggtgcaggta tgattggctc tatcatggcc
     1981 tggcatttga acaacaagct gggtcggcaa gacctggtga ttgttgaccg tatcacgcat
     2041 gaaaaccagt ggcaaaacct ggtgcacagg cactatgccg aatatctgga taaagaccag
     2101 ctgtttgagt ttttagaaga taatgatggc atcactgcag tggttcatat gggggcgatc
     2161 agtgccacca ccgagcgcga ctttaacaag ctggtcgcag acaacatgca ctactcgcag
     2221 gatttatggt cctggtgcac tgagtatgaa gtgccgtttt tttatgccag ctctgcggcg
     2281 acgtatggcg gcggtgaaca gggctatgac gatgccagca ttgagaacct gcgcccctta
     2341 aatggctatg gctattccaa acactttttt gaccagtggg tgttacagca agtcgcacaa
     2401 aaacaggcta cacctccggc ttgggcaggc ttcaagttct ttaatgtcta cgggccaaac
     2461 gagtatcaca aagaacgcat ggccagcgtg gcctaccaca cctttaacca gttcagtgaa
     2521 accggcacca tgcgtctgtt taaaggcacc aaggccggtg tagaagacgg catgcaactg
     2581 cgtgattttg tctatgtgaa agacgtggcc gatgtgatgg gatttttcct ggagggtgcg
     2641 ttaaaaaaca aaccggttac cagcgcgatt tacaatatcg gcactggtca ggcacgcagc
     2701 tttaaagacc tggcaaccaa cgtcatgacc agcatgtctc gcgaacccaa catcacttat
     2761 attgatatgc cgcaagacct tcaaggcaaa tatcagtatt ttacgcaggc agagatgaaa
     2821 aaactgcgcg atgcgggtta taaaaagccg tttaccacac tggaagaagg cgtgaaggat
     2881 tacgtgcaga actatttgct gcaagatgac ccttacgcat aactccaaca aagacagtga
     2941 gcgatatgaa ccatattgaa tatctaaaag gcgagcacgc cgcacatatg gcgatgttcc
     3001 aggcgctgga gccattgttt ccgctgatag gtgatgttgg caacttattg cgcgactgta
     3061 ttcagcgtgg cggtaaagtg ttgctgtgcg gcaatggcgg cagcgcggca gacagtcagc
     3121 atattgcggc tgaaatcgtc ggtcgcttta agaaagagcg taaaggcctg ccttctatcg
     3181 ccttgacaac ggatacgtct atcctgactt cagtcggcaa cgattatggt tatgactata
     3241 tttttgcacg tcaggtagag gccttgtgcc gcccagaaga cattttgatt ggcctgacca
     3301 ccagcggcaa tagcgccaac gtggtccgcg ccattgaaac tgccaatgag attggcgcca
     3361 ctacgattgg cctgacaggc ggcagcggtg gcaaaatgaa tgcactgtgc acacataaca
     3421 ttgtcgtgcc ttccaacgtt acagcccgca ttcaagaggc gcatattttt atcggccatt
     3481 gcctgtgtga aatcctggaa tcctgatgac tggcaacgcc ttacatagtg acctggcaac
     3541 cgtggttgcc cattttggtg aacaaacgca acacgcactg gttatcggcg atgtcatgct
     3601 ggaccgctat ctgatgggtg aagtcaaccg tatttcgcct gaagcaccag tgccagtcgt
     3661 gctaatcaaa tcagaacaac aacgtgctgg cggcgccgcc aacgttgccg ccaacctggc
     3721 actgctggga gtcactaccc acatgatagg cctgattggc aatgatcatg aagggcagcg
     3781 tttattggca gccatgtcgg aatacggcat caatacgcag gccatgatca ccacaccggc
     3841 acgccccacc attgccaaaa cccgaatttt gggcgggcat cagcaaatga tgcgtctgga
     3901 tcaggaagtg accggagcgc tctcagacaa agaggcagcg caagtcatgc aggcgactca
     3961 aacggcattg caacaacatc ccgcgctgat tattttgtcg gactacgcca agggcttgct
     4021 gactgagcag gtctgccagc aagtgataga ttatggccgc cagcacaaca ttccggtatt
     4081 ggttgatccc aaaggacgta actacgataa atatcgtggt gcaactgcgc tgacaccaaa
     4141 taaaaaagaa actgctgagg cctgtgatac gcagatgagt gatgctgcac taatagacaa
     4201 agcgtgcaca cttaaaacat cgttgtcgtt agactttctg gccgtcaccc gcggtgaaga
     4261 aggcattaca ctgattgccg accaaaccac tcacctgagc gcaaccgcca aacaggtgtt
     4321 tgatgtctca ggcgcaggcg ataccgtgat tgccacatta gctgccggcc tcatgcatgg
     4381 cttaagttct ttggacagct tggcactcgc caaccatgct gcaggtgtcg ttgtcggcaa
     4441 ggtcggtacc gtacccatta ccaaagctga ccttctggac gccattgcca ctgcgcaggg
     4501 cagtcaacag gcacacaaaa tcagtgaatt acctgactta ctgcaaaaag tggctaactg
     4561 gaaacagcag ggccagaaaa ttgtgtttac caacggctgt tttgatttgt tgcatgctgg
     4621 tcatgtgacc tatctggaag gcgcaaaaaa acacggggac aggctgattt tgggcctcaa
     4681 taccgaccgc tctgtttctg cactcaaagg cccgacacgc cctgtcgtca atgagaaaga
     4741 ccgtgcccgc gtcttggcag cactggagtc cgtggatgcg gtcatcctgt ttgacgaaga
     4801 caccccgctc aacctgatca acgccataca acccaatatc attgccaaag gcagcgatta
     4861 cactgctgac caggttgtcg gtggcaagga agtgttgtca tggggtggag aaattgcctt
     4921 gatagacttg gtcgaaggcc gtagcaccac caacctgatc aaaaaaatgc acgcatgaac
     4981 aagattctgg tgatgggccc atcatgggtg ggcgacatgg tactggcgca aagcctgttc
     5041 aagcagctca aaatcgagca gacggattgt cagattacgg ttgccgcccc cgcatggaca
     5101 cttcccttgc tggagagaat gcccgaggtg tcacaagcga ttgcattgcc ctttaaacat
     5161 ggcgacctgg cattacgtga acgcttcaag cttggccgcg aactggctaa agaaggcttt
     5221 actcaggcta ttttactcac caacagcttg aaatctgcca ttttaccttg ggccgccaga
     5281 attccagtgc gcacaggctt caaaggcgaa atgcgttatg gcctggtgaa tgacatgcgc
     5341 cccttggaca agtccacact caagcaaacc gtgcagcgat ttgtcgcctt gggcttaccc
     5401 aggcatgcaa cgctccctga gtcactgcca caaccaacct tgttggcaaa tgcagctaat
     5461 gctccctcgc ttttgcgaaa gtttgattta cccacccacg gcacggtatt aggcctctgc
     5521 cctggcgcag aatacggtga agccaaacgc tggccgacag aatactatgc agaagtagcc
     5581 aatactgcgc ttaaccaagg ctggcaagtc tggttgtttg gctctgaaaa agaccagccg
     5641 gtcacaacta gtattcagca gcagacgggg cagcgctgcg tagacctggc gggcaaaact
     5701 aaacttggcg aagccataga cctcatgtca ctgtgccaca ccgtgatcag caacgattcc
     5761 ggcttaatgc acgtcgcggc agcactggat aaaaagctga tcgcgattta cggctcatct
     5821 gaccccaaac acaccccgcc catgcatccg caagccgtga ttgaatactt gggacttaca
     5881 tgtagtccct gttttgaacg cacctgcccc ttaggccatc tcaactgttt gcgacaactc
     5941 aaacccgctt ctgtcctagc caaactgcct tggaaaattc tggaaaccgg caaatgacct
     6001 ccttttcttt tttgcggaaa atatcgcaat cacagtggct caacatttat ctcttagcag
     6061 cctttttaat tgcctgcaag atcatgtaca accagcatgg ctgggtcaac aatgattcac
     6121 tactgtactt tgagcaggca cggctaatcg ctgccggtca gctgcaacaa gcttttggtt
     6181 tattcacctg gatactttat cccgggcttc tggcaatcat tcacaaactg acagggctga
     6241 gcattcatgt ttctgccatc agcttgaacg tcctatgttt tgtgatcttt gttgcaggtt
     6301 ttcagcggtt gttaatagaa gcaggagcca aactgcgcac actgcactgg ggcatgctat
     6361 tactcttttc aacacaatat attgttggtg acgtactcgg catgttattg cgtgacgaag
     6421 gattttgggc ggcttatacc tggggattgc tattttggtt acgtgcgctg aagtcgcaac
     6481 actggaaaca tattctgcag tttcaagctg tcatggcggt cgccgtgctt ttccgtatcg
     6541 aatcatcagc ctatttatta gcgcttccgc tatgcgcatt actcttgcca caactaactt
     6601 ggctgcaacg tctcaagtta tggctaaaag caaactcgct agcactatta acgaccgtga
     6661 tcgtagcaat tggattgctg gcaggcgttt tacaacaaga gcaactggga cggttgcagg
     6721 aagtatttac ccaaatagca cacctgttca ctgagcgcat taccttcatc aaccagaaag
     6781 ccgccatcct gggtcatcag gtgttggggg aagatcttga tcaatacggt acatttagcc
     6841 tatggtcaag cctggtcttg attgcgatat ttaagaccct caaagtggca ggcctgccag
     6901 ctttggcagt gatcctctgg ccaaggcaaa cctggtggtc aaaactgcca gaagtaaccc
     6961 gttatctggt catctctacg ctgaccatca gtttcgtagt gagcctggtt atcattttta
     7021 atgtgtttat attgtcgagt cgttatgtga ttgcaagcgg tattgtcatg ctacttctgg
     7081 cggcgttcgc catcacagag tggcaacaac gctggcctaa atgggtaagc aaaagctatg
     7141 tgctactact tgcagtactt ttcatgtact ccttgtttga caaacatgag attgacttgg
     7201 accgagtagc cgtcgactat attgcacaga ttaaccctca gcacaaaccg gcgttttatg
     7261 ataccgaaaa tgccagattt tatgctgacc aaccctatac tgaccgcatt ttaggccgtg
     7321 tcgcgtttcc acaattggtc aacaagaatg agattgatca atacgactac ttcatgatca
     7381 ccatcagtaa agatccggca gacattgctt atgaacggca agcgctgcaa gtgttaggtg
     7441 agcatcaata caaacttgtt aatacgctct acggctggcg gaacaaaacc aaagcactta
     7501 tttttgttcg tcatccagag aacaagccaa actaatacgt taaaatacgt aaccatataa
     7561 ttaaaaaaac aggcacctca tcgatatgac gcccctacct ctcaatcttg cattgactgt
     7621 gccagccaac ttgcgcgcat catttaagca actattacgt ttcagcctgc tttgggtggt
     7681 gctatttgcc tgtaccgcca atgtctgtaa cagttttttc atcaagtggg gattccggga
     7741 cgattcacac tatgcgtcac tgctcagcgt gattgatggg agtgcttacc agccttatgt
     7801 ttaccgttca acagtgcccc gcctcgcaga cttggccatt cagcatattc cggctgaaaa
     7861 acaagagcaa ctctttcacc gcatcatctc ttcagacaaa cttaaacatc acttttttaa
     7921 tgatgtgcct ctagagaaat ggacgccacg catagcgctg gattatcacc tgatgtatgg
     7981 actcaccttg ctgagttttt tgattacgct ggtttatctg cgcaaaatat ttttatactt
     8041 ttctgaaaac tattcagcct ctatcctggc tgttgtattg tttagcctga tgtaccctct
     8101 cacattcaag cgcggtggct atttttatga cttcttcgaa ttattgggta tcacggtcag
     8161 cacatattac tttctgacaa accgcaaact cttggcctcc atcctgttga tgttgacggc
     8221 gttcaacaaa gaaaccgctt ttgttgcggc gtttggcttc tttttcctac acgccaaaga
     8281 ttatcccatg caaaaacgcc tggcgtatt
//
LOCUS       DATMSK010000188         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_225670,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000188 DATMSK010000000
VERSION     DATMSK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225670"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VNZ84_06215"
     CDS             <1..387
                     /locus_tag="VNZ84_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWU66588.1"
                     /translation="ETTDRGNRFNQPAGHLEEGETIIAAAIRETLEETAYDFTPEALV
                     GIYHWQHPLNGITYLRFAFTGKLGAHYPEQALDDGILRTVWKTEAEIAAEGELMRSPQ
                     VLLCVQDYRAGKRYSLDILKHLGQAL"
     gene            384..1478
                     /gene="mnmA"
                     /locus_tag="VNZ84_06220"
     CDS             384..1478
                     /gene="mnmA"
                     /locus_tag="VNZ84_06220"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWU66589.1"
                     /translation="MSKPHVIVGLSGGVDSSVTALLLKQQGYQVTGLFMKNWEDDDTD
                     EYCSSRQDLIDAVSAADRIGIDIETVNFSAEYKERVFANFLQEYQAGRTPNPDILCNA
                     EIKFKAFLDHAMGMGADLIATGHYAQVRENPIQPGNFQLLKADDGSKDQSYFLSRLNQ
                     AQLSKTLFPLGKYLKREVREIAREHGLANAEKKDSTGICFIGERPFQEFLQRYLPRHP
                     GVMRTPEGKVVGEHEGLMYYTIGQRQGLKIGGSRESNGEPWFVAAKHMASNELIVVQG
                     HDHPLLQSDGLKAGQLHWISGEEPRTNWVYAAKTRYRQPDAPCEVDAVDAEEATIRFG
                     QHQWAITPGQSVVVYESNVCLGGGIITGAI"
     gene            1559..1888
                     /locus_tag="VNZ84_06225"
     CDS             1559..1888
                     /locus_tag="VNZ84_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66590.1"
                     /translation="MAYVLKNKEGEIVAASPDTQVAADWVEVENTNSEYLDFLDNELK
                     KHHTFRESDIQLARVLEDLISILIDRSLISFTDFPSAAQKRLNERQSLRHKNKLNLLG
                     DDDVAHL"
     gene            complement(1891..>4007)
                     /locus_tag="VNZ84_06230"
     CDS             complement(1891..>4007)
                     /locus_tag="VNZ84_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWU66591.1"
                     /translation="TVTDTRTKAPETGINRTLPITTITSEQLQRVQPSNIFDAVRSVP
                     GVSITGGPRPSGMTFNIRGYGDNEDVMVKVDGVPKGFEKYRMGGTFIEPELLKSIEVQ
                     RGPQITSGSGSLGGTVNATTKNAEDFLKPGQKYGGKAKFGYGNNSDEYSRSYLLYARP
                     DERVDILYNYSNRQSNNMTLGDGTKLDNSAIESISHLLKVSVFLTEDLQLVTSVVKFD
                     DTGLQPYDATGGQPGFFGNVIRSVDDLTWSETIHYDPESQWVNLKATVGAGHTDLSDL
                     AKPGMSSVINPSITGCNGTTYTPDPSQVSRCPGNATDTYQYKTKTVDISNQAIFFERG
                     NLNLSLLAGYQYNASDREISRTFDNPLSVAQSRYPEGFNASAPPGSKSFNAIYIQPKI
                     EVDQFTVTPGYRQDRYQVEAAGGTLALLQPYDQKSKIRFKEETWSLGLAYDAFAKNNP
                     DKLTFYSNYGQGFRPPLIDEYFTQGPFSRCRALLMPISGPDSLICGDLYQPQRSETTE
                     VGVSVQTPHLLNTDIWFSGKLNFYHTYTSHLLLSLREEADGTITQNGWERRNGVEVES
                     FLQYHGWYMRSGYSRINGEIFNGLAFAPLFTAPGNALNINVGAELTKHLEVNVTYRKV
                     SERDILISGDGTVNSRYVFGQQDGYEMWNAGIRLKLNEQLTLRLIGENLKNEEYRLDG
                     SMGGLGMYGPGRNVKFLVEMTY"
BASE COUNT         1037 a          989 c          960 g         1021 t
ORIGIN      
        1 gaaaccacag accgcggtaa tcgcttcaac cagccggccg gacatctgga agagggcgaa
       61 acgattattg ccgcggcaat tcgtgaaacg ctggaagaaa cggcttacga tttcacgcct
      121 gaagcattgg taggcattta ccattggcag catccactca atgggattac ctatttgcgt
      181 tttgccttta ccggcaaatt gggtgcgcat tatccggagc aagctttaga cgatggcata
      241 cttcgcaccg tatggaaaac tgaagccgag attgcggctg aaggcgagtt gatgcgtagc
      301 ccacaagtct tgctgtgtgt gcaggattac cgggcaggga aacgttattc tttagacatt
      361 ttgaagcatt tgggccaagc attatgagta agccacatgt catcgtcggg ttgtccggcg
      421 gtgttgattc ttccgtcacc gccttgttgc tgaagcaaca aggttaccag gtcaccggtt
      481 tgtttatgaa aaactgggaa gacgatgata ctgacgaata ctgttcttca cgccaggatt
      541 tgatcgatgc cgtttcggcc gctgacagaa taggcattga catcgaaacc gtgaatttta
      601 gcgctgagta caaagagcga gtgttcgcta actttttaca ggaataccag gctggccgca
      661 cgccaaaccc ggatattctg tgcaatgccg agattaaatt caaagccttt ctggatcacg
      721 ctatgggcat gggtgctgac ttgatcgcca ccggtcatta tgcgcaagtg cgtgaaaatc
      781 cgattcaacc cggtaatttt cagctactga aagccgatga cggtagtaaa gatcaaagct
      841 actttttgtc acgcctgaac caggcacaac tgtcaaaaac gctgttcccg ctgggcaaat
      901 acctcaagcg tgaagtacgt gaaattgccc gcgagcacgg tttagccaat gccgagaaaa
      961 aagactccac cggtatttgt tttattggtg agcgtccgtt tcaggaattt ttgcagcgtt
     1021 acctgcctcg tcacccgggc gtgatgcgca cgccagaagg caaagtggtg ggagagcatg
     1081 aaggcttgat gtattacacc ataggtcagc gtcagggcct taaaattggt ggcagtcgcg
     1141 aaagcaacgg cgagccctgg tttgtggccg ccaaacatat ggccagcaat gagttgatcg
     1201 tggtgcaagg tcacgaccat cctttgctgc aaagtgatgg tctcaaggct ggacaattac
     1261 attggatcag cggtgaagaa ccgcgcacta actgggtcta tgctgccaaa acacgctatc
     1321 gccagccgga tgcgccttgt gaagtcgatg ctgtggatgc cgaggaagcc accatccgtt
     1381 tcggccagca ccagtgggcg atcacgccag ggcaaagcgt agtggtgtat gaaagcaatg
     1441 tctgcctggg cggcggcatt attacgggtg ccatttagcc acctctgagg catggttatt
     1501 gcttgcactt gcagaatata atgacaacga acctccaagt gacgaggaat gacaccttat
     1561 ggcctatgta ttaaaaaata aagagggtga gattgttgca gcaagcccgg atacgcaggt
     1621 ggcggcagac tgggtagagg tggaaaacac gaatagtgaa tacctcgatt ttttagacaa
     1681 tgagctaaaa aaacaccata ccttccgtga aagtgacatt caactggcac gtgtattgga
     1741 agacttgatc agcatcctga ttgatcgtag cctgatcagc tttaccgatt tccctagtgc
     1801 cgcacaaaaa cggttgaatg aacgccagag cctgcgtcat aaaaacaagt taaatctgct
     1861 tggcgatgac gatgtggcgc acttgtaata tcaataagtc atttctacca aaaacttcac
     1921 atttctaccg ggaccgtaca tccccaagcc tcccatgctg ccatcaaggc ggtattcctc
     1981 atttttcaag ttttccccaa taagccttaa agtcagctgt tcgttgagct tcaaacggat
     2041 accggcattc cacatttcat agccatcctg ctgaccgaac acataacggc tattcactgt
     2101 cccatccccg cttatcagaa tatcccgctc agagactttt ctgtaagtca cattcacctc
     2161 aaggtgtttg gtcaattcgg cacccacatt aatatttaat gcattacctg gcgccgtaaa
     2221 taatggggca aacgccaaac cgttaaagat ctcaccgttg atacgtgagt acccgctgcg
     2281 catataccag ccatgatatt gcaagaaact ctcaacctct acgccatttc tgcgctccca
     2341 gccattctgg gtaatcgtgc catctgcttc ttcgcgcagg gaaagcaata agtgagaggt
     2401 gtaggtatgg taaaaattaa gctttccaga gaaccagata tccgtattca gcagatgtgg
     2461 cgtttggaca ctcacgccaa cctcggtagt ctctgaccgc tgtggctggt ataggtcgcc
     2521 acagatcaat gaatcaggac cactgattgg catcaataac gctcggcaac ggctaaatgg
     2581 cccttgtgtg aaatattcgt ctatcaaggg tgggcgaaag ccttgcccat aattactgta
     2641 aaacgtcagc ttgtcaggat tattctttgc aaacgcatca taagccaatc caagactcca
     2701 ggtctcttcc ttaaatctga tcttactttt ctgatcataa ggctgaagca aagcgagtgt
     2761 cccgccagcg gcttcgactt gatagcggtc ttgacgatac cctggggtca ccgtaaattg
     2821 gtccacttca attttaggtt gtatgtaaat ggcattaaat gatttgctac ctggcggtgc
     2881 agaagcatta aatccttcag ggtatctgga ttgggcaacg ctcaaaggat tatcaaacgt
     2941 acggctaatt tcacggtcac tggcgttgta ttgatagcct gccagtagtg aaagatttaa
     3001 gttgccacgc tcaaaaaaaa tggcctgatt gctgatatca accgtcttgg ttttatactg
     3061 ataggtatct gttgcattac cgggacatct gcttacctgg ctcgggtctg gtgtatacgt
     3121 tgtaccatta caacctgtga tactcgggtt aatcactgat gacatgcctg gctttgccag
     3181 atcactcagg tcggtatgtc cggcaccgac tgtcgctttt aaattcaccc attgactctc
     3241 cggatcataa tggatggttt cagaccaggt caggtcatct accgaacgaa tcacatttcc
     3301 aaagaatcct ggctgccctc cggtcgcatc ataaggttgc aggccagtat catcaaactt
     3361 caccaccgag gtcaccagtt gcaaatcctc cgtgaggaac acactcacct ttaataaatg
     3421 cgagatagac tcaatcgccg agttgtccag tttggtccca tcacctaatg tcatgttatt
     3481 ggactggcgg ttagagtagt tataaagaat atccactcgc tcgtccgggc gcgcatagag
     3541 taaataagag cgcgaatact catcgctgtt attcccatag ccaaacttgg ccttacctcc
     3601 atacttttga cccggcttta agaaatcctc cgcgtttttg gtcgtggcat tgacggtgcc
     3661 tcctaacgaa ccagagccgc tcgtaatttg cggaccacgt tgtacttcta tgcttttcag
     3721 caattccggc tcgatgaacg tgccacccat gcggtatttt tcaaagccct tgggaacgcc
     3781 atcgactttc accatcacat cttcgttatc gccataaccg cgaatattaa atgtcatgcc
     3841 actaggacgt gggcccccag tgatggaaac cccaggcacg gacctgaccg catcaaaaat
     3901 attactgggc tgtacacgct gcaattgctc actcgtaata gttgtaatgg gcaacgtgcg
     3961 attaatgccc gtctcggggg ctttggttct cgtatcggtg accgtca
//
LOCUS       DATMSK010000189         8201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_461144,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000189 DATMSK010000000
VERSION     DATMSK010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461144"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1021
                     /gene="fabD"
                     /locus_tag="VNZ84_06235"
     CDS             98..1021
                     /gene="fabD"
                     /locus_tag="VNZ84_06235"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWU66592.1"
                     /translation="MSKFAFFFPGQGSQSVGMMQGFENHAVVKQTFDEASTVLGVDFW
                     KMATEANDAINLTENTQPIMLTAGVATWRVYQALGGKLPAVMAGHSLGEYTALVAADA
                     ISFADALPLVKFRAQVMQQAVPEGQGAMAAILGLDDDTVRAVCNEAAQGEVVQAVNFN
                     SPGQVVIAGNKAAVERGMELAKAKGAKRALPLPVSVPSHCDLMKPAAEKMREYLQNLT
                     VQAPLVPVLHNADVAAYNDADQIKDALVRQLYSPVRWVETVQQLAAQEILTGAECGPG
                     KVLAGLAKRIVAEMSCVALVNDESVQAFIAQ"
     gene            1033..1773
                     /gene="fabG"
                     /locus_tag="VNZ84_06240"
     CDS             1033..1773
                     /gene="fabG"
                     /locus_tag="VNZ84_06240"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWU66593.1"
                     /translation="MSNQQVALVTGASRGIGAAVAQALGKQGAFVIGTATSASGAEAI
                     TAHLKAAGVNGVGFALDVTQPEQIDAVLKQIAEQYGEVSILVNNAGITKDTLLMRMKD
                     EDWDAVLNTNLKSVFRMSQAVLRPMMKARQGRIINISSVVGHMGNAGQTNYAAAKAGM
                     TGFTKSMAQEVGSRGITVNCVAPGFIDTDMTAELPEEIKAKMLERIAVGRLGQVDEIA
                     ATVAFLASPAAAYITGETIHVNGGMYCA"
     gene            1892..2128
                     /gene="acpP"
                     /locus_tag="VNZ84_06245"
     CDS             1892..2128
                     /gene="acpP"
                     /locus_tag="VNZ84_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWU66594.1"
                     /translation="MSDIEQRVKKIVCEQLGANEADVKNTSSFVDDLGADSLDTVELV
                     MALEEEFDCEIPDEEAEKITTVQQAIDYVNANLK"
     gene            2180..3412
                     /gene="fabF"
                     /locus_tag="VNZ84_06250"
     CDS             2180..3412
                     /gene="fabF"
                     /locus_tag="VNZ84_06250"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWU66595.1"
                     /translation="MTKRRVVVTGLGVVSPVGIGTQTAWNNLVAGQSGITRITKFDPT
                     PFASQIAGEVKGFNAEDFIPAKDARRMDTFIQYGLAAGLEAFRDSGLEVTEANAERIG
                     VTIGSGIGGLGLIESTNDSYDEGGPRKISPFFIPGTIINMISGNLSIMLGLKGPNIAV
                     VTACTTGTHSIGEAARMIEYGDADVMVAGGAEAAITELSVGGFASARALSSRNDDPAT
                     ASRPWDKDRDGFVIGEGAGVLVLEEYEHAKARGAKIYAEVAGYGLSADAYHMTAPNMD
                     GPRRSMVNALKNAGVSPDQVQFMNAHGTSTPLGDTNETNAIKATFGDHAYKLVVNSTK
                     SMTGHLLGGAGGLESVFTVLSVYHQVSPPTINLFNQDPECDLDYCANTARDLQIEYAL
                     KNNFGFGGTNGSLLFKRV"
     gene            complement(3475..3978)
                     /gene="tpx"
                     /locus_tag="VNZ84_06255"
     CDS             complement(3475..3978)
                     /gene="tpx"
                     /locus_tag="VNZ84_06255"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HWU66596.1"
                     /translation="MASVTLKGNPVAVGGKFLATGDTAPAFQLADAKRNLVGLDAYAG
                     KRKILNIFPSIDTPTCATSVRTFNKEASSASNSVVLCISADLPFAQSRFCGAEGLENV
                     VTLSTFRDTAKFATDYGVFITDSSLAGLTARAVVVLDENNKVLHSELVGEIADEPNYA
                     AALAVLK"
     gene            complement(4069..4575)
                     /gene="tpx"
                     /locus_tag="VNZ84_06260"
     CDS             complement(4069..4575)
                     /gene="tpx"
                     /locus_tag="VNZ84_06260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HWU66597.1"
                     /translation="MPNATALLDDPILVEDNLPGLGQALPYFQLVNQALEDVSIEAFS
                     GQRKILHFFPGIDTATSANSVRILDKLAASLPNTALLHISADLPFTMARFYATENLTN
                     GIHLSTMRGRDMLKNYGVLMMTSKLAGLPARVLMVADENDRVLHVELVKELTNEPDYE
                     AAIEALQG"
     gene            4760..>8201
                     /gene="dnaE"
                     /locus_tag="VNZ84_06265"
     CDS             4760..>8201
                     /gene="dnaE"
                     /locus_tag="VNZ84_06265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWU66598.1"
                     /translation="MEALQTPTLQPAFIHLRCHSEYSIVDGTVRIDDYVKAAKSDAMP
                     AIALTDLANLFGAVKFYKAARGKGLKPVLGCDVWVENTIQRDQPHRLLLLVQNQQGYR
                     NLCELLSRAYLENQYRERAEIKPEWLQEKNAGLLMLSGFAAGDVGQALQAGNQALAVQ
                     YAGQWTKTFAGRYYLEVQRVHEAASAEAYTQEQLIQQTVLLAQQLDLPIVATHPVQFQ
                     TADDFMAHEARTCIAEGYVLADNRRPRRFLASQYVKTQAEMQALFADMPLALQNSVEI
                     AKRCNLTLTLGKNYLPDFPTPNGESLDAYLVDQSKAGLEHRLAYLYPDEQQREAKRAE
                     YEERLQFECNIIVQMGFPGYFLIVADFINWAKNNGVPVGPGRGSGAGSLVAYSLGITD
                     LDPLKYNLLFERFLNPERVSMPDFDIDFCMEGRDRVIDYVKRKYGLASVSQIATFGTM
                     AAKAVIRDVGRVLDLPFNFVDGIAKLIPMELGITLESALEKEPQLQERREKEEEVAEL
                     LSLALRLEGLVRNIGMHAGGVLIAPGKISDFSPVYCQPNGESLVSQYDKDDVEAVGLV
                     KFDFLGLRTLTILNMALENANAQREQDELPPLSFETIPLDDKRSYHLLKTANTTAVFQ
                     LESRGMKDMLKQAKPDLFEDIIALVALYRPGPMDLIPDFCRRKHGTQRVEYPHASTES
                     ILKETYGIAVYQEQVMQIAQVVAGYSLGGADLLRRAMGKKKPEEMVKQREIFNEGALK
                     NGLTLQQSNDLFDLLEKFAGYGFNKSHAAAYALVAYHTAYLKAHYPAAFLAATMSADM
                     NNTDNVHTFFDDCGANHVEVLPPDINASIYQFKPLNAQQVLYGLGAIKGTGLAAIELI
                     LASRQAQGPFKDIFDFCQRLDLRKVNRRVIESLIRAGAFDKLEQNRAALLAGLDTAIS
                     VAEQGAAHAGQDCLFGGVIETRHELPAIAAWSPEQALQEEKTALGFYLSGHPYLSYKA
                     QLGPFIKGNLSELAPQDQPKLLAGVVMGVRVRMTQRGKMAIVTLDDAVGRVDVVIGAE
                     LLMQHAPWIKDDQLLVVEGRISHDEFSGGIRVSARKLYDLAAVRNRFAQMLTLSCNGS
                     SDVQRLMNVLTPYRYAGEEQKRCPVKVDYHNQSAQVSLMLGDDWQVVLHDDLLHNLQG
                     W"
BASE COUNT         2018 a         1863 c         2248 g         2072 t
ORIGIN      
        1 ggttttacct ggggctctgt actggcgaag tattaacttt atacccataa gcatgatcaa
       61 catgcgtcac tttagctaac acactacttg aaaatatatg tctaaatttg cttttttctt
      121 tcctggccaa ggctcacagt ctgttggcat gatgcaaggc tttgaaaatc atgcggttgt
      181 taaacaaacc tttgatgaag cctccacagt attgggtgtt gatttctgga aaatggcgac
      241 tgaagccaat gatgcgatta atctcaccga aaacacacag cccattatgt tgaccgcagg
      301 agtggctact tggcgtgttt accaggcatt aggtggaaaa ttgcctgctg tcatggcggg
      361 acatagtctt ggagaatata ccgctttggt ggctgctgat gctatttcat tcgctgatgc
      421 gttgccctta gtgaaatttc gtgcccaggt aatgcagcaa gctgtgccgg aagggcaagg
      481 ggcgatggcc gcgattctcg gcctggatga tgatactgtg cgtgctgttt gcaatgaagc
      541 agcgcaaggc gaggtggtgc aagcggtaaa ctttaactcg ccaggccagg ttgtgattgc
      601 cggtaataaa gcagccgttg agcgtggcat ggagttggcc aaagccaaag gtgccaagcg
      661 tgctttacca ctgcctgtga gtgtgccatc gcactgtgac ttgatgaagc cagctgcgga
      721 aaaaatgcgc gagtatttgc aaaacctaac agtgcaggcg ccattagtcc cggtgctgca
      781 taacgctgat gtggctgctt ataatgatgc tgatcagatc aaggatgcgt tggtgcgcca
      841 attgtatagt ccagtgcgct gggttgagac cgtgcagcaa ttggctgcac aagaaatatt
      901 aactggtgcc gagtgcggtc caggcaaggt gctcgctggt ttagccaagc gtattgtggc
      961 tgagatgagt tgtgtggcgc tggtaaatga cgaatctgtg caagcattta ttgcgcaata
     1021 aggggaagaa tgatgtcaaa tcaacaagta gcattagtga ctggtgcgag cagaggaatt
     1081 ggtgctgccg ttgcgcaagc tttaggtaag cagggtgcat tcgtgattgg aacagcgacg
     1141 tccgcgagcg gtgctgaagc cattacagcc catttgaagg cggctggcgt gaacggtgtt
     1201 ggctttgcgc tcgatgtgac gcagccagaa cagattgatg cggttttgaa acagattgcc
     1261 gagcaatatg gagaagtgtc cattctggtg aacaatgccg gaattaccaa agacactttg
     1321 ctgatgcgga tgaaagatga agactgggat gctgtattaa atactaattt gaagtctgta
     1381 ttccgcatga gccaggctgt cttacgccca atgatgaagg cacgccaagg ccgtattatc
     1441 aatatttcca gtgtggttgg tcatatgggt aatgccggtc agaccaatta tgcggcagcc
     1501 aaagccggga tgacagggtt taccaagtcg atggcgcaag aagtcggtag tcgcggtatt
     1561 acagtcaact gtgtcgcacc aggctttatt gacactgata tgacggctga gttgccagaa
     1621 gagattaagg ccaaaatgct ggagcgcatc gccgtcggtc gcttaggtca ggtagatgaa
     1681 attgccgcta cggtcgcttt cctggcttca ccagctgccg cttatatcac gggtgaaacc
     1741 atccatgtca atggtggcat gtactgcgca taagatatgc taactgtagt tttcatatca
     1801 ttttaaataa ttgttttttg gcattctggg caagatttgt tagaatgccg gtttactgaa
     1861 cacgacactt tatcaataag gggtatttta gatgtccgac atcgaacaac gcgtaaagaa
     1921 aatcgtttgt gaacaactgg gtgcaaacga agctgacgtt aagaacacat catctttcgt
     1981 agatgatctg ggtgcagatt ctctggacac cgtggaattg gtgatggctt tggaagaaga
     2041 attcgattgc gaaattcctg acgaagaagc tgaaaaaatc acaaccgtgc agcaagcgat
     2101 tgactacgtc aacgctaacc tcaagtaatt cattgactta attctttaat cagccctcgc
     2161 aagagggctg atttctatta tgacgaaacg cagagtagta gtaaccggct tgggcgtggt
     2221 gtctccagta gggattggta cccaaaccgc ttggaacaat ctggtcgctg gtcaatcagg
     2281 cattacccgt atcactaaat ttgatcctac cccgtttgcc tcgcaaattg caggtgaagt
     2341 aaagggattt aatgctgagg attttatccc ggccaaggat gctcgccgta tggatacctt
     2401 tatccagtat ggcttggcag ctggcttgga agcattccgt gactcaggtc tcgaggtgac
     2461 tgaagccaat gccgagcgta ttggcgtcac tatcggttcc ggtattggcg gtttgggttt
     2521 gatcgaatct acgaatgaca gctatgacga aggtggcccg cgcaagattt cgccattctt
     2581 tattcctggc accatcatta atatgatttc tggtaacctg tcgatcatgt tgggtctgaa
     2641 aggcccgaat atcgccgtgg tgacggcttg tacgacaggc acacatagca ttggcgaagc
     2701 cgcccgtatg attgagtatg gcgatgcaga tgtcatggtt gcaggtggcg ctgaagcggc
     2761 gattacagag ctgagcgtag gtggctttgc ctctgcgaga gccttgtcta gtcgcaatga
     2821 tgatcctgca acggctagcc gtccctggga taaagaccgc gacggttttg tcattggtga
     2881 aggtgcaggg gtattggtgc tcgaagagta tgagcatgcc aaagcacgtg gagccaagat
     2941 ttatgctgaa gtagctggtt acggtttaag tgccgatgcc tatcacatga ccgcgccaaa
     3001 catggatggc ccacgtcgct ccatggttaa cgcgctcaaa aatgcgggag tgagccctga
     3061 ccaagtgcag tttatgaacg cacatggaac ctcaacgccg ctgggtgaca cgaacgaaac
     3121 caacgctatt aaagccactt ttggtgatca tgcttataaa ctggttgtga attcaactaa
     3181 gtctatgact ggccacttgc tgggcggtgc tggtggtctg gagtctgtgt ttactgtgtt
     3241 atccgtgtat caccaggtat ctccaccaac gattaacttg tttaatcagg atcctgaatg
     3301 tgatctggat tactgcgcta atactgcgcg tgatttgcag attgaatatg ccctgaagaa
     3361 caacttcggt tttggtggta ccaacggtag tctgctgttc aagcgcgttt aagcaattga
     3421 atgacaaata aaaaagccag catctgctgg cttttttatt tgcgttcgag tggcttattt
     3481 caatacagcc agcgcagcag cgtagttagg ctcatcggcg atttcgccta ctagttcgct
     3541 atgcaagact ttattgtttt catccagtac gacaaccgcg cgagcagtta agccagccaa
     3601 gctgctatcg gtgataaata caccgtagtc agtggcaaat ttagcggtgt cacggaaggt
     3661 tgacaaggtc accacgtttt ccaggccttc tgcaccacag aagcggcttt gcgcgaacgg
     3721 caggtcggca gaaatgcata acacaacact attgctggcg ctactggctt ccttgttaaa
     3781 tgtgcgtaca gaggtggcac atgtcggtgt gtcaatgctt gggaaaatgt tcagaatctt
     3841 gcgcttgcca gcataggcat ccagaccaac caggttccgt ttggcatctg ccagttgaaa
     3901 tgcgggagcg gtatcacccg ttgccaaaaa cttgccgcca acggcgacgg gattaccttt
     3961 aagcgttacg cttgccatag tgttctccaa aacaatcaat gatgggtggt taatgttcta
     4021 ctttaacttg tcgggcagtg gcaatcaacc acataaaaag taagggaatc atccctgcag
     4081 cgcttcaata gctgcttcgt agtcaggctc attggtgagt tctttgacta actcaacatg
     4141 cagcacacgg tcattttcat cggcaaccat gaggacgcgt gcaggcaagc ctgccagttt
     4201 gctggtcatc atcagcacgc cataattttt aagcatgtcc cgcccgcgca tggtagacaa
     4261 gtgtatccca ttggtcaggt tttccgtggc gtagaagcgc gccattgtaa acggcaaatc
     4321 tgcagagata tgcagaagag ccgtgttagg caagctggcg gccaatttat ccagaatgcg
     4381 gacactattc gcgctggtgg cagtatctat gcctgggaaa aagtgcaata ttttgcgttg
     4441 cccgctaaat gcctcaatcg aaacgtcttc cagggcctgg tttacaagct ggaagtaggg
     4501 cagtgcttgt cccaaaccgg gtaaattgtc ttccaccagt atcgggtcat cgagtaaggc
     4561 agtggcgttg ggcataatta aacagtcaac ttacaaaaac gactgacatc atagagcata
     4621 acctggccgg aattaacttt tttatcactt gttaaccagt agggcagatg cgtattgtgc
     4681 cgggttttga gtgatgtcaa ccacattatt tgtagcgggt atgcgtacaa aaaaggagag
     4741 ggcgggtaaa ataaaagcca tggaagcgct tcaaactccc accctacaac ctgcatttat
     4801 tcacctgcgt tgccacagtg agtattccat tgtggatggc actgtacgta ttgacgatta
     4861 cgtcaaggca gccaaaagtg acgcgatgcc tgcgattgcc ctgactgacc ttgccaacct
     4921 gtttggtgca gtcaagtttt acaaggctgc ccgcggcaaa ggcttaaaac cagtgctcgg
     4981 atgcgatgtt tgggttgaga atacgataca gcgagatcag ccgcatcgtt tgttattgct
     5041 ggtgcagaac cagcaaggct atcgtaatct gtgcgagttg ctctctaggg cttacttaga
     5101 aaaccagtac cgtgaacggg ccgaaatcaa acctgaatgg ttgcaggaaa aaaatgccgg
     5161 tttattgatg ctgtcagggt ttgcggctgg cgatgttggt caggcgttac aagctggaaa
     5221 ccaggcattg gctgtgcagt atgccggtca atggacaaag acttttgctg gtcgatacta
     5281 ccttgaggtg caacgtgtac atgaggctgc cagcgccgaa gcttacacgc aagagcaact
     5341 gatccagcaa accgtgctgc tggcacagca actggatctg ccgattgtcg ccacacatcc
     5401 cgtacagttc caaacggctg atgattttat ggcacacgag gcgcgtacct gtattgccga
     5461 gggctacgtg ctcgcggaca accggcgtcc acgccgcttt ctcgcatcac aatatgtcaa
     5521 aacacaggcc gaaatgcagg ccttgttcgc cgacatgcct cttgctttac aaaactcggt
     5581 agaaattgcc aaacgatgca acctgacact gacattgggt aaaaactacc tgccagattt
     5641 tccaacaccc aatggcgaaa gcctggatgc ctatctggtt gaccaatcta aagccgggct
     5701 ggagcatcgg ttggcgtatt tatatccaga tgagcaacaa cgggaagcaa agcgcgctga
     5761 gtatgaagaa cgcttgcagt ttgagtgcaa tatcatcgta caaatgggtt tccccggata
     5821 ttttctgatc gttgccgact ttattaactg ggctaaaaac aacggtgtgc ctgttggccc
     5881 tggccggggt tccggtgctg gctccttggt ggcttacagt ttaggaatta ccgacctcga
     5941 tccgctcaaa tacaatctac tgtttgagcg gttcctcaac cctgagcggg tgtccatgcc
     6001 cgacttcgat attgactttt gtatggaagg acgagatcgc gtcatcgatt acgtcaagcg
     6061 caagtatggc ttggcatcgg tgtcacagat cgccactttc ggtaccatgg cggccaaagc
     6121 ggtgatccgc gatgtaggtc gcgtgcttga tttgccgttc aactttgtgg atggcattgc
     6181 caagttaatc ccgatggagc tgggcattac gctcgagtca gcccttgaga aagagccgca
     6241 actgcaagag cgccgcgaaa aagaggagga agtggcagag ctactctctc tcgcactgcg
     6301 cctggaaggc ctggtgcgta acattggtat gcacgccggt ggggtgttga ttgcgccggg
     6361 taagatctct gatttctcgc cggtatattg ccagccaaat ggcgaaagcc tggtcagtca
     6421 gtatgacaaa gatgacgtgg aagcagtggg tttggtcaaa tttgacttcc tgggcctgcg
     6481 tacgctgacc atcctcaata tggcgctgga aaatgccaat gcacagcgtg agcaagacga
     6541 gttgccaccc ctgagttttg agacgattcc gctggatgac aagcgcagtt accatttgtt
     6601 gaaaaccgcc aataccacgg ccgtattcca gctggaatct cgtgggatga aggacatgct
     6661 taagcaagcc aagccggact tgtttgagga cattattgcg ctggtcgctt tataccgccc
     6721 tggtccaatg gacctgatcc cagacttctg ccgccgtaag catggcacgc aacgtgtgga
     6781 gtatccacat gcctcgactg agtctatcct gaaagaaacc tatggcattg cggtatatca
     6841 ggagcaggtg atgcagattg cccaggtggt ggctggttac agcctcgggg gtgccgactt
     6901 gctgcgtcgc gccatgggta agaaaaagcc tgaggaaatg gtcaagcagc gcgaaatttt
     6961 caacgaaggc gcgcttaaaa atggtttgac cttgcagcag tcgaatgacc tgtttgactt
     7021 gctggaaaaa tttgccggct atggtttcaa caagtcacat gcggcggcgt atgctttggt
     7081 ggcatatcat actgcgtacc ttaaagcgca ttatcctgcg gccttcctgg cggccaccat
     7141 gtcggccgat atgaataaca cggataacgt gcataccttt tttgatgatt gtggcgcaaa
     7201 ccatgtcgaa gtgctgccgc cggatattaa cgccagcatt taccaattta agccattgaa
     7261 tgcgcaacag gtgttgtatg gtttgggggc gattaaaggc acgggcttgg ctgcgattga
     7321 gctgattctg gcttcacgcc aggcgcaggg accattcaag gatatttttg atttctgcca
     7381 gcgactggat ttgcgtaagg tcaaccgccg ggtgattgaa tcgctgattc gtgccggtgc
     7441 ctttgataag ctggagcaaa accgtgccgc cttgctggct ggattggata cggcgattag
     7501 tgtggccgag cagggtgcag cgcatgctgg tcaggattgc ctgtttggtg gtgtgataga
     7561 aacccgtcat gagttgccag cgattgctgc ctggagtccc gagcaggcac tgcaagaaga
     7621 aaaaacggct ttaggttttt atttaagcgg ccatccttac ctgagttaca aagcacagtt
     7681 ggggccattt attaaaggca acctcagtga acttgcacca caagaccagc ccaaactgtt
     7741 ggctggagtg gtgatgggtg tacgtgtacg tatgacccag cgcggcaaaa tggcgattgt
     7801 gacgctggat gatgccgttg ggcgtgtgga tgtcgtgatt ggcgcagaat tgctgatgca
     7861 gcatgcgcca tggattaagg atgaccagtt gctggtggtg gaagggcgca tcagtcatga
     7921 tgagttttcc ggtggaatcc gcgtcagtgc cagaaagctt tacgacttgg ctgccgtacg
     7981 taaccgattt gctcaaatgc tcacgcttag ttgcaatggt tcatcagacg tgcagcgcct
     8041 gatgaatgta ctgacaccct atcgctacgc cggtgaagag caaaagcgct gtccggtgaa
     8101 agtcgattat cataaccagt ccgcccaggt cagcctcatg ctgggcgatg actggcaagt
     8161 cgtattgcat gatgacctgc tacataattt acagggctgg t
//
LOCUS       DATMSK010000190         6631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_296905,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000190 DATMSK010000000
VERSION     DATMSK010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296905"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..808
                     /locus_tag="VNZ84_06270"
     CDS             56..808
                     /locus_tag="VNZ84_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWU66599.1"
                     /translation="MKALVAIKRVVDYNIQVRVKADGSDVDIEGVKMGVNPFDENAIE
                     EAIRLKEKGIVTEIVAVTIGSAANQDILRHALAMGADRAMLVETQETLHSLSVAKVLK
                     AVVEREQPKLILLGKQAIDDDAGQTGQMLAALLQLPQATYASQITIEGDQATVVREID
                     GGTQTLAVSLPAVITADLRLNEPRYVKLPNLMMARKKPLENVALDTLNVSLTHNIQQL
                     NVATPPTRPAGIMVKDVNELLSKLRTHEGIIA"
     gene            805..1734
                     /locus_tag="VNZ84_06275"
     CDS             805..1734
                     /locus_tag="VNZ84_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWU66600.1"
                     /translation="MRTLIIAEHDGQHLNDQVARAVTAAQQWQQPVDILIAAAQPDTL
                     AAQAAAIQGVSSVLVASDAALQHPTAEHLAEIIVPLATNYTVVLAAHSAFSKNVLPRV
                     AALLDVNMISDVLQIVSTNTYVRGIYAGNVLATVQSSDSKQVLTVRGSKFAAAASGAT
                     AEVKPVAAPAAPAGSRWVAEAFNKSERPPLAAAKVVVSGGRALGSSEAFAEVLAPLAD
                     KLHAAIGASRAAVDAGYAPNDLQVGQTGVVVAPELYIAVGISGAIQHAYGMKDSKVVV
                     AINQDPEAQIFKIADYGLVADLFEAVPQLTAAL"
     gene            1777..2556
                     /locus_tag="VNZ84_06280"
     CDS             1777..2556
                     /locus_tag="VNZ84_06280"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HWU66601.1"
                     /translation="MSKKYSTETVLSVHHWNDTLFSFKTTRDPGLRFENGQFVMIGLE
                     VDGLPLTRAYSIASPNYEEHLEFFSIKVPNGPLTSRLQHLKVGDPLLVSRKPTGTLIT
                     SDLKPGKNLYFFSTGTGLAPFMSLIQDIEVYDRFEKVILIHGVRYVNELAYEDFITKE
                     LPNNEFFGEEVRNKLIYYPTVTREPFKNMGRLTDLVENGKLFTDIGLPPLNPETDRAM
                     ICGSPAMLVDTAAMLDKRGFKVSPRIGELGDYVIEKAFVEK"
     gene            2595..3062
                     /locus_tag="VNZ84_06285"
     CDS             2595..3062
                     /locus_tag="VNZ84_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU66602.1"
                     /translation="MTATPAVVLESANLSDLSALTALLALLFEQEHEFKPDLQAQQKG
                     LSLILQHPDSGRLLVARHQGEVIAMVSLLFTISTALGGRVALLEDMVVHPNFRGNGIG
                     SQLLRFAIDDARRQQVLRITLLTDDDNHAAQCLYEHHGFQPSSMQAWRLVLKA"
     gene            complement(3045..4496)
                     /locus_tag="VNZ84_06290"
     CDS             complement(3045..4496)
                     /locus_tag="VNZ84_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU66603.1"
                     /translation="MSLPTQALTFEILTQRYGERYKWMVLLIVGLGTIAGVLCTSSFN
                     VAVPALTHTFHLGQDHVQWAMTAFLAAMTIGMLPAAWWLERYGLRRVFMTALWLLIAA
                     SVAGFYAQTFVQVVIARLIQGLAAGVLQPLGIMALMRLFPPHIQGRASGILIVGIAFT
                     PAIAPSISGWLLDQFGWHAIFLLGVPFALLALVSAQVWLPAPRQKEVPLFDWPGLGWL
                     SAATIGLIEFVSSLHHSGVVAIWTWGYGMLTVVSLICYIWHAHRLPHAIIQLHLFSRP
                     TFSRGTFVAFAYGFGLFASTYLIPVFLQNAMAFSATDAGLLLLPSGIALVLTIPLAGR
                     MADRFSPRRITLAGLAIFGASFLVFVFVAMRVQYWEIVAATVVGRIGLGLILPALNLA
                     TLHEMEPQHLGQSSVVVSYARQLGGVVGVTMMAVFMEWREQVWGSVAPGVFQAYAQGF
                     LLLSVVFVLAILVAMGMKDARRQDNLAPSGLED"
     gene            4737..5387
                     /locus_tag="VNZ84_06295"
     CDS             4737..5387
                     /locus_tag="VNZ84_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66604.1"
                     /translation="MKRWMLYTCLALLTQGALADEALDSDLASLQHAWAKATYQTPKD
                     QQEPAFKALTEQAHQLSAKNPEASEAMIWEAITLSGYAKSMGPLHPVNALKAAEQARD
                     LLLASIKTNPQALNGSAYTTLGSLYFRVPGWPIGFGDKKKGREYLEKALQFNPTGIDS
                     NYFYADYLRAQGEYSQAVTYYQKALQAPARPGREDADAGRKQEVEEGLRLAQSKLK"
     gene            5415..5972
                     /locus_tag="VNZ84_06300"
     CDS             5415..5972
                     /locus_tag="VNZ84_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HWU66605.1"
                     /translation="MLLKPLAIRLASSLLLSLPLLASAEGFYGGLNLGVGMPNLKTPN
                     GVDKDTQAVAGVVAGYKFNPYFAVEGQYTGIGKVTDNVSGSAKADALSLTAVGILPLN
                     DAFDLYGKFGVAATKSKVSSGLNAYNDATHTGATYGLGAEYHFDKAISMRVGWDHYKA
                     EVDVNGGGSKSFDSNVTTVGVVYHF"
     gene            complement(6045..>6631)
                     /locus_tag="VNZ84_06305"
     CDS             complement(6045..>6631)
                     /locus_tag="VNZ84_06305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU66606.1"
                     /translation="AVAQALAEKHPEVDVLVNNLGIFEPKAFEEIPDADWIRFFDVNV
                     LSGVRLARLYLPYMKQQDWGRILFISSESGVQIPPEMIHYGMTKSAQIAVARGLAESL
                     AGTQITVNSILPGPTRSRGVGDFVEAMAQAEQKSAERFEKEFFEHVRPTSLIKRFATT
                     EEVASLVTYIASPLSSATNGAALRVDGGVIKSAF"
BASE COUNT         1799 a         1828 c         1614 g         1390 t
ORIGIN      
        1 acctggctta aaatgctcgg ttacgcttga acatccagat gaaggcaaac gttttatgaa
       61 agcattagta gcgatcaaac gggtcgttga ttataacatc caagtccgcg tcaaggcaga
      121 cggttcggat gtggatattg aaggggtcaa aatgggcgtg aacccatttg acgaaaacgc
      181 gattgaagaa gccatccgcc tcaaggaaaa aggcatcgtc acagaaattg tcgccgtgac
      241 cattggcagt gctgccaacc aggatattct gcgccatgca ctggcgatgg gcgctgaccg
      301 cgccatgctg gtcgagacac aggaaacctt gcattcactc agcgttgcca aagtgctaaa
      361 agccgtggtt gagcgcgaac agcccaagct aatcttgctg ggcaagcaag ccattgatga
      421 tgatgcaggc caaactggcc agatgttggc agcattgttg caattgccac aagccaccta
      481 tgcttcacaa atcacgattg aaggtgacca ggcgactgtg gtgcgtgaaa ttgatggcgg
      541 cacacaaaca ctggccgtca gcctgccagc cgtgattact gctgacttac gcctgaacga
      601 gccacgctat gtcaaactgc ctaacctcat gatggcgaga aagaaacccc tggaaaatgt
      661 ggcattagat acactaaatg tttccctcac gcacaatata caacagctca acgtcgctac
      721 accgcctaca cgccctgccg gcatcatggt gaaagatgtc aatgagttat tgagtaaact
      781 gcgtacgcac gaagggatca tcgcatgaga acactgatta ttgccgaaca cgacggccag
      841 catttgaatg accaggtcgc gcgcgcggtc actgccgcac aacaatggca gcaaccggta
      901 gatattctga ttgctgccgc tcaacccgat acattagcgg cacaagctgc agccatccaa
      961 ggggtgagca gtgtattggt tgccagcgat gcggctttgc aacatcccac tgcagagcac
     1021 ctggcagaga tcattgtgcc tttggcgacc aactacacag tcgtactggc tgcacatagt
     1081 gcattcagca aaaatgtact gccacgtgtg gctgcattac tggatgtcaa tatgatttcc
     1141 gatgtattgc agatcgtcag taccaacact tatgtgcgtg gcatctacgc gggcaatgta
     1201 ctggcaaccg tgcaaagcag tgacagcaaa caagtactca ctgtacgtgg cagcaagttt
     1261 gccgccgccg caagcggcgc gactgcagaa gtgaaaccag ttgcagcccc ggctgcgcca
     1321 gccggcagcc gttgggttgc ggaggccttt aacaaatccg agcgtccacc attggctgct
     1381 gccaaagtcg tggtctctgg tggccgagca ctaggcagct ccgaagcctt tgccgaagta
     1441 ttggcgccat tggccgataa actgcatgcc gcgattggtg ccagccgcgc cgccgtggat
     1501 gccggttatg cgccgaatga cttgcaagtc ggccagaccg gtgttgtcgt tgcgcctgaa
     1561 ctgtatattg ccgtgggtat ttccggcgca atccagcatg cctatggcat gaaagatagc
     1621 aaagtcgtgg tggcgatcaa ccaggatcca gaagcacaga tcttcaagat cgcagattat
     1681 gggctggttg ccgacctgtt cgaggccgtg ccgcaactga cagctgcgtt ataatgcatc
     1741 ctttaaatta aacccttcag aaacctactt catctcatga gtaaaaaata ttctaccgaa
     1801 acggtgttga gcgttcatca ctggaatgac acactgttca gcttcaaaac cacacgtgac
     1861 ccgggcttgc gctttgaaaa cggccagttt gtcatgatcg gcctcgaagt ggatggcctg
     1921 ccgctgaccc gtgcttacag tattgccagc cctaactatg aagagcacct ggagtttttc
     1981 agcattaaag tgcctaatgg cccgctgacc tcccgcctgc aacacttgaa agtgggtgac
     2041 ccgctactgg tcagccgcaa gcctaccggc acgctgatca cctctgacct gaagccaggc
     2101 aaaaacctgt acttcttctc taccggtact ggcctggcac cgttcatgag cctgatccag
     2161 gacatcgaag tgtatgaccg ttttgagaaa gtcatcctga ttcacggcgt gcgctacgtg
     2221 aatgaactgg cctacgaaga tttcatcact aaagaattgc cgaataacga attttttggc
     2281 gaagaggtgc gtaacaaact gatttactac ccaaccgtca cccgcgaacc attcaagaac
     2341 atgggccgcc tgactgacct ggtagaaaat ggcaaactgt ttacagatat cggcctgccg
     2401 ccattgaatc cggaaacgga tcgcgccatg atttgtggta gcccagccat gctggtcgat
     2461 accgcggcca tgttggacaa acgtggtttc aaagtctctc cccgcattgg cgagttgggc
     2521 gactacgtga ttgaaaaagc cttcgtcgag aaataacaga ccatgcaaga cggctgtcat
     2581 ggagagatga tcgcatgaca gccactcctg cagttgtatt ggaatcggca aacctatctg
     2641 atttgtctgc cttaaccgcc ctgctagcct tgctattcga gcaagagcac gaattcaaac
     2701 cagacctcca agcccagcaa aaaggcctat ccctgattct gcaacacccg gacagtggcc
     2761 gactcttagt ggccagacac caaggtgaag tgatcgccat ggtgagcctg ctgtttacca
     2821 tttccaccgc attgggtggc cgtgtcgctt tactcgagga catggtggtt catccaaatt
     2881 ttcgcggtaa cggcataggc tcgcaattac tgcgctttgc cattgatgat gcgcgcaggc
     2941 aacaagtact acgcatcaca ttgctgacag atgacgacaa tcatgccgcc cagtgtttat
     3001 atgagcacca tggcttccag ccctccagca tgcaagcctg gcgattagtc ctcaaggcct
     3061 gatggcgcaa gattatcttg gcgccttgca tccttcattc ccatcgccac caatatcgcc
     3121 agtacaaaca ccacagacag caataagaag ccttgtgcat aggcctgaaa cacgcctggc
     3181 gccactgagc cccatacctg ctcccgccac tccataaaca ccgccatcat ggtcaccccg
     3241 acaaccccgc ccaactgccg ggcatagctg accactacag atgactgccc cagatgctgg
     3301 ggttccattt catgcaaagt ggcgagattt agcgcaggca agataagccc caacccaata
     3361 cggccaacca cggtcgcagc aacgatctcc cagtattgca cccgcatcgc cacaaacaca
     3421 aagactaaaa acgaggcacc gaatatggcc aagccagcca aggtaatacg tcgtggtgaa
     3481 aatctgtctg ccatccggcc cgccaacgga atggtcaaca ccagcgcaat ccctgagggc
     3541 aacagcaaca aaccagcatc tgtcgcactg aaggccatcg cattctgcaa gaaaaccggg
     3601 atcaggtaag tagaagcaaa tagcccgaaa ccataagcaa aagcgacaaa cgtgccacgg
     3661 ctaaaggtgg gcctggaaaa caggtgcaat tgaataatcg catgtggcaa ccgatgtgca
     3721 tgccagatat aacagataag agaaacaacc gttaacatgc catagcccca ggtccagatg
     3781 gcgaccacac cactatgatg caagctggag acaaattcga tcaaacctat tgttgcggca
     3841 gacaaccagc ctaatcctgg ccagtcaaac aatggcacct ctttttgacg cggtgccggt
     3901 aaccagactt gagcactgac caatgccaac aatgcaaacg gcacaccgag taaaaaaatg
     3961 gcatgccagc caaactggtc aagtaaccag ccactgatgg aaggtgcaat ggccggtgta
     4021 aacgcaatgc cgacaatcag gatgccggag gcacgcccct ggatatgcgg cggaaacaac
     4081 cgcatcaacg ccataatccc cagtggttgc aacacccccg cggccaagcc ctgtatcaat
     4141 ctggcaatca ccacctgtac aaaagtctgc gcataaaatc cggccacact ggcagcaatc
     4201 aacaaccaaa gcgctgtcat aaatacgcga cgcaaaccat agcgctccag ccaccaggca
     4261 gcgggcaaca tgccgatggt catcgcagcc aggaaagccg tcatggccca ttgcacgtga
     4321 tcctgcccca gatgaaaagt atgtgtcaac gcggggaccg ctacattaaa actggaggtg
     4381 cagagtacgc cggcaatggt acccaagccg acgatcagca gcaccatcca tttataacgt
     4441 tcgccatagc gctgggtcaa tatttcaaaa gtaagggctt gtgttggcag ggacattttt
     4501 cttcacggct tgaggcaaga tgacagcgac catagcatgc attttttgca tagtcacctg
     4561 ctgagaccct gcaaaaaaag cagggttcgc gcacccagga aaagttcacc tggctgacag
     4621 tcccatagaa ttcaagttaa gcccatgttt ttattgaaaa tattattacc ttgattcatt
     4681 cagtctgctt ggcattgata ttgcaaaaac taaagtattt aaaacgagga gcgaatgtga
     4741 aacgttggat gttatatacc tgcctagctt tgctgaccca aggcgcattg gcagatgaag
     4801 cgctggatag cgatcttgcc agcttgcaac atgcctgggc aaaagccact taccaaacac
     4861 ctaaggacca gcaagaacct gcgtttaaag cactgaccga acaggcacat caactatccg
     4921 ccaaaaaccc ggaggccagt gaggccatga tttgggaagc cattacatta agtggttacg
     4981 caaaaagcat ggggccctta catccggtga atgcgctaaa agccgccgag caagcgcgcg
     5041 atttgttact ggccagcatc aaaacaaatc cacaggcact caatggttca gcttatacaa
     5101 cactgggatc gttgtatttc cgcgtccctg gatggccaat cggttttggc gacaagaaaa
     5161 aaggccgcga atacctggaa aaagcactgc aattcaatcc aacagggata gacagcaact
     5221 atttctacgc agactaccta cgcgcccaag gcgaatacag ccaggcagtc acgtattacc
     5281 agaaagcgtt acaggcacct gcccgccctg gccgtgaaga tgcagatgcc ggacgcaaac
     5341 aggaagtgga agaaggcctg cgcctggcac aaagcaaatt gaaataatcc taatcacctt
     5401 tacttggagt cgccatgtta cttaaacctt tagctatccg gctcgcaagc agcctgttgt
     5461 tatctctgcc attactcgcc agtgcggaag gattttatgg cgggctcaac ctcggtgttg
     5521 gcatgccaaa cctaaaaaca cccaacggcg tagacaaaga tacgcaagct gtcgctggcg
     5581 tggtcgctgg ttacaagttt aacccttact ttgcggtaga aggccaatat accggcattg
     5641 gtaaagtgac tgacaatgtc agcggtagcg ccaaagccga tgcactcagc ctgacggcag
     5701 tcggcatttt gccactcaat gatgcttttg atctctacgg aaaatttggt gtcgcagcga
     5761 ccaaaagcaa agtatcttct ggcttaaatg cttataacga tgccacgcac acgggtgcaa
     5821 cctacggctt gggagcggaa taccattttg acaaagcgat ttccatgcgc gtcggttggg
     5881 atcactataa agccgaagtg gatgtgaatg gtggtggcag caaatctttt gattccaatg
     5941 tcacaacagt cggcgtggtc taccacttct aggcatcagg tatatccctc tctcaatacc
     6001 tgatgctcgc ctgccagggc cgatcaccct ggcaggctac ttccctaaaa agcgctttta
     6061 ataacgccac cgtccacgcg caatgccgcg ccgttcgtag cagaggataa agggctggca
     6121 atataagtca caagactggc aacttcttca gtagtcgcaa accgcttgat caatgacgtc
     6181 ggccgcacat gctcgaaaaa ttctttttca aaacgctccg cggacttttg ctctgcttgc
     6241 gccatggcct ctacaaaatc ccctacacct cttgagcgcg tcggacccgg caatatacta
     6301 ttgaccgtaa tttgcgtgcc cgccaaggac tccgccaagc cgcgtgccac ggcaatctgc
     6361 gccgacttcg tcatgccgta gtggatcatt tctggtggaa tctgcacacc actctcgcta
     6421 gaaatgaaca ggatacggcc ccaatcctgc tgtttcatat aagggagata cagtctggct
     6481 aggcgcacgc cgctcaacac attgacatca aaaaaacgta tccagtcagc atcgggaatc
     6541 tcttcaaacg cctttggctc gaagataccc aggttattaa ccaagacatc cacctcggga
     6601 tgcttctcag ccaaggcctg tgctacagcc g
//
LOCUS       DATMSK010000191         6554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_414478,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000191 DATMSK010000000
VERSION     DATMSK010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414478"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="infC"
                     /locus_tag="VNZ84_06310"
     CDS             complement(<1..478)
                     /gene="infC"
                     /locus_tag="VNZ84_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWU66607.1"
                     /translation="MAQDKDTRINGDITAPQVRLIGVDGEPLGVMALREAFAKAEEAD
                     IDIVEIAPNAAPPVCRLMDYGKFKYAESKRLHEQKLKQKQVQIKEVKFRPGTDDGDYN
                     IKLRNIIRFLGDGDKAKITLRFRGREITHQEFGLALLKRLEADLTEHALVEQYPKME"
     gene            complement(522..2444)
                     /gene="thrS"
                     /locus_tag="VNZ84_06315"
     CDS             complement(522..2444)
                     /gene="thrS"
                     /locus_tag="VNZ84_06315"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWU66608.1"
                     /translation="MPVIRLPDGSSRSFEQPVTVADVAMNIGAGLAKAALAGKVTYTG
                     QEAKLVDTSHLIEQDVDLAIITDKNDEGLEVIRHSTAHLLAYAVKELFPDAQVTIGPV
                     IDNGFYYDFSYKRPFTPDDLAAIEKKMAELAKKDEKVERKVLPRDEAVAYFKSINEHY
                     KAEIIASIPSNEDVSLYSEGNFTDLCRGPHVPNTGKLKAFKLMKLAGAYWRGDSNNEM
                     LQRVYGTAWRNKDELEAYLHMLEEAEKRDHRKLGKQLDFYHMQEDAPGMVFWHPRGWV
                     IWQEVEQYMRAKFKEYDYQEVRTPTVLDKTLWEKSGHWQNYRDNMFVTQSENRDYAVK
                     PMNCPGHIQIFNSSLHSYRDLPLRLAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFC
                     MESQVEAEVADFIKMLYEVYKDFGFDDVIVKLSTRPEKRVGTEEAWDMAETALANAIK
                     ANGLEFEYQPGEGAFYGPKIEFTLKDSLGRMWQCGTIQLDPNLPERLGAEYVAEDNTR
                     QRPIMLHRAIVGSMERFLGILIENFAGTMPVWLSPVQVMVLNISDNQADYVRQVVEKL
                     KKNGIRCDFDLRNEKITYKIREHSLQKLPYLIVVGDKEMQNGHVAVRTRKGEDLGVMS
                     VDAFVAHIQQDIQNRV"
     gene            complement(2649..2725)
                     /locus_tag="VNZ84_06320"
     tRNA            complement(2649..2725)
                     /locus_tag="VNZ84_06320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2689..2691),aa:Val,seq:gac)
     gene            2796..3482
                     /locus_tag="VNZ84_06325"
     CDS             2796..3482
                     /locus_tag="VNZ84_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWU66609.1"
                     /translation="MVSAYNIPMRNIASSPIPDSLLDTIIDTLATQEYCVIDQFLSNT
                     LVSDLRAIALERQQLGQMHQAGTSQSAITNLNLRGDQIAWLEADDPHPAIQHYLALLD
                     TIQQAINRHLMMGLESFETHFAIYPENSAGYATHIDQFRPHRHQLAKGARTLTSIIYL
                     NDAWPESAGGQLRLYLDEHTEKPAADARHLDIAPLGGKLVLFLAARFWHEVLPASLPR
                     ISVTGWFKNR"
     gene            complement(3483..4658)
                     /locus_tag="VNZ84_06330"
     CDS             complement(3483..4658)
                     /locus_tag="VNZ84_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HWU66610.1"
                     /translation="MVGLAVVTHVSYADDEEQFQSWLVTVRQEALALQIAPAAVDATL
                     NQVTYLPRVIELDRKQPEFVTSFSAYVNRRINPRVVSKGKQAMQDYQGVLYVVEELYQ
                     VPREVLVAFWGLETNFGGFMGDIPLASALATLSYEGRRAEFFKRELFNLMRVVEREQR
                     YDSPVVGSWAGATGHMQFMPTTLLKYGVDADADGKIDIWGSYPDIFSSAANYLAQAGW
                     QPGKPVSLQVALPAGFDYSQAQWQLNKPVSEWVKAGVAGVPDSALNLPQAAILLPQGY
                     DGPAYMVFPNFDVIMQWNRSVNYALAVGLLSQQLRQDTYTLMMPPEPPALSYQQIWSL
                     QESLNAKGFDCGTPDGFPGARTQAAIRRYQASKGLPQDGYAGAEIYTRLTAQVQSAQ"
     gene            complement(4717..5547)
                     /locus_tag="VNZ84_06335"
     CDS             complement(4717..5547)
                     /locus_tag="VNZ84_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWU66611.1"
                     /translation="MRVLQIGCGGLGRLIAEATLAQGHALTIVRRSHQPVPEGAQVLY
                     ANVMTGEGLSALANVQADILLCCLAPVDGQSYQETYVDGLRHVLASAQQDTLNHVFFV
                     SSTGIYGEHQGEWIDEETVAKPVDDSGHIMLAAEQLLEGLTCGHTALRVSGIYGATRL
                     YLLRLLQNRERWPTQTHWTNRIHELDVAAAVVYLYQQVAKGQALPAHCILSDGVPAAQ
                     HEVLQWIATQKQVPGPEAPPLLPQSGKRIRNRFLQQSGFKLQFADYRAGYASILSNLK
                     "
     gene            complement(5557..6147)
                     /locus_tag="VNZ84_06340"
     CDS             complement(5557..6147)
                     /locus_tag="VNZ84_06340"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWU66612.1"
                     /translation="MSESKTVNQDQHLIETTVSSEILAKGKMLTVRYDEARLPNGVIS
                     GREYVIHPGAVVVIALTQEGGVVLERQFRYPLKQVFIELPAGKIDPDEPVLTTGQREL
                     LEETGYTAQEWVKLGVQHPCIGYSNEVIHIYLATGLSLGAYARDIDEAMDVFVWPFEQ
                     VMQAIEAGEITDSKTMSAMLLAERYLRKHPISGVDS"
     gene            complement(6166..>6554)
                     /locus_tag="VNZ84_06345"
     CDS             complement(6166..>6554)
                     /locus_tag="VNZ84_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWU66613.1"
                     /translation="AYVIAHEVGHHVQNLLGTSAKVQQARQQARSEAESNGYSVRLEL
                     QADCYAGVWAHHADQQFRILEEGDIEEALRAATAIGDDALQKQAQGYVVPDSFTHGSS
                     AQRVRWFKQGLQSGRVNDCNTFKNSV"
BASE COUNT         1635 a         1892 c         1427 g         1600 t
ORIGIN      
        1 cttccatttt aggatattgc tccaccaatg catgctctgt taaatctgcc tcaaggcgtt
       61 ttaacaatgc caaaccaaat tcctggtgcg taatttcgcg accacggaag cgcaaagtaa
      121 tctttgcttt atcaccatca cccaaaaagc ggatgatatt gcgcaattta atattgtaat
      181 cgccgtcatc ggtgccaggt ctgaatttga cctctttaat ctgcacctgt ttttgtttca
      241 gtttttgctc atgcagacgc ttgctctcgg cgtatttgaa tttgccataa tccatcaaac
      301 ggcacacagg gggtgcagca tttggcgcaa tttcaacaat atcaatatca gcttcttccg
      361 ctttggcaaa ggcctctctc agagccatca cacctaacgg ttcgccatct acaccaatca
      421 aacgcacttg tggcgcagta atgtcgccat tgatgcgtgt atctttatct tgagctataa
      481 caatccccta acaataaaca aagccgcacg aatacccgaa attagacccg gttttggatg
      541 tcttgctgta tgtgcgcgac aaacgcatct accgacatca cacccagatc ttcacctttt
      601 ctggtacgta cggccacatg accattttgc atttctttgt cgcccaccac gatcaggtat
      661 ggcaactttt gcaaactatg ttcgcgtatt ttataggtaa tcttctcatt tctcaagtca
      721 aaatcgcatc gaatgccatt tttcttcaat ttttcaacca cttggcgtac gtaatccgcc
      781 tggttatcag aaatattgag caccatcacc tgtactggcg acaaccagac cggcatcgtc
      841 cccgcaaagt tttcaatcaa aatacccagg aaacgctcca tagaaccgac aatcgcgcga
      901 tgcaacatga tcgggcgctg acgagtattg tcttcagcga catactccgc acccaggcgc
      961 tcaggcaggt taggatcaag ctgaatcgta ccgcattgcc acatacggcc cagagagtct
     1021 ttaagggtaa actcaatttt cggaccgtaa aaagcaccct cacctggctg atactcaaac
     1081 tcaaggccat tggctttaat ggcgttggcc aaagcggttt ctgccatatc ccaggcttct
     1141 tccgtgccca cgcgtttttc tgggcgggta gacaacttga caatcacatc gtcaaaacca
     1201 aagtccttgt agacttcgta gagcatctta ataaagtctg ccacctcggc ttcaacttgc
     1261 gattccatac agaaaatatg ggcatcatcc tgggtaaaac cgcgcacccg catcaagcca
     1321 tgcagcgaac ctgaaggctc attacggtgg caggatccaa actccgccaa gcgcaacggc
     1381 aaatcacggt aactgtgcaa actgctatta aaaatctgga tatggccagg acagttcatc
     1441 ggcttcacag catagtcgcg gttttccgat tgagtcacaa acatgttgtc gcggtaattt
     1501 tgccagtggc cggatttttc ccacagggtt ttatccagca ctgtcggcgt acgcacttcc
     1561 tggtaatcgt actccttgaa cttggcacgc atgtactgct caacttcttg ccagatcacc
     1621 caaccgcgtg gatgccagaa caccatacca ggcgcatctt cctgcatgtg gtaaaaatca
     1681 agctgcttac ccagcttgcg gtggtcacgc ttttcagcct cttctagcat gtgcaggtaa
     1741 gcttccaact catccttgtt gcgccaggcg gtgccgtata cgcgctgcag catttcatta
     1801 ttgctgtcac cgcgccagta ggcaccggcc agtttcatta acttaaaggc cttgagtttg
     1861 ccggtgtttg gcacatgcgg accacggcac aagtcagtga agttgccttc agagtacaaa
     1921 gatacatctt cattgcttgg aatgctggca atgatctcgg ctttgtaatg ctcgttaatg
     1981 cttttgaagt aagccactgc ttcatctcgc ggcagcacct tacgctcgac tttttcgtct
     2041 tttttcgcca gctcagccat tttcttttca atcgcagcca ggtcgtccgg cgtaaacggg
     2101 cgcttgtagc tgaagtcata gtaaaagcca ttatcaatca ccggaccgat ggtcacctgc
     2161 gcatcaggga acagctcttt caccgcgtaa gccagcaagt gcgcggtgga gtgacgaatc
     2221 acttccaggc cttcatcgtt cttatcggta atgatggcga ggtccacatc ctgttcaatc
     2281 aaatgactgg tatcgacgag tttagcttcc tgacctgtgt acgtgacctt acccgccagc
     2341 gcagctttcg ccaggccagc gccaatattc atcgcaacat cggccacggt caccggctgt
     2401 tcaaaacttc tggatgaccc atcaggcaag cggattacag gcatgactcc ctctctttaa
     2461 acattaaaca accaaacaaa acaagcacat ggcacctgtt tttggtcaaa cctcgattat
     2521 ctcacagcgc acgaaaattt cgcaatcaca agacaaaact catgcatgac ttcaaactag
     2581 ccttaacgcc gatcagcagg gggctaaaaa aacaaaaacc accagaatat cctggtggtt
     2641 tttttatttg gtagtcgcga atggactcga accatcgacc cccaccatgt caaggtggtg
     2701 ctctaaccag ctgagctacg cgactaaatg tatttgcaca atgtactgca aatcaataag
     2761 gttgcgcatt ctatccgaaa gacatcggtt cggcaatggt atccgcttat aatataccga
     2821 tgcgcaacat cgcctcctca ccaatccctg atagcctgct tgacaccatt attgatacct
     2881 tggcgacgca ggagtattgc gtgattgatc aatttttatc caataccctc gtaagcgact
     2941 tgcgcgcaat cgcactcgaa cggcaacagt taggccaaat gcaccaggcg ggtaccagcc
     3001 aatcagccat caccaacctc aacttgcgcg gagaccagat agcctggctg gaagccgatg
     3061 acccacaccc cgcaatccag cattacttag cgcttctgga tacaatacag caagccatca
     3121 accgccactt aatgatgggg cttgagagct ttgaaacgca ttttgctatt tacccggaaa
     3181 actctgctgg ctatgccacc catatcgacc agttccgacc gcatagacac cagcttgcca
     3241 agggagcacg cacactcacc tcgatcattt atttaaacga tgcctggcca gaaagcgcgg
     3301 gcgggcaact tcgtctctat ctggatgaac acaccgagaa gcccgctgca gatgccagac
     3361 atctggacat cgccccactc ggaggcaaac tggtattgtt tttggccgcc agattctggc
     3421 atgaggtatt gcccgccagc ctgccacgca tcagcgtcac cggctggttt aaaaaccgtt
     3481 agctattgcg ctgactgaac ttgtgcagtc agccgggtat atatctcagc gcctgcgtaa
     3541 ccatcttgcg gcaaaccttt gctcgcctgg taacgccgga tggccgcttg cgtcctggcc
     3601 cctggaaaac catccggtgt tccgcaatca aatcccttgg cattgagcga ctcctgcaaa
     3661 ctccatattt gctggtagct cagcgctggc ggctcgggtg gcatcatcag agtataggta
     3721 tcctgacgca gctgctggct aagcaaaccg actgccagcg catagttcac tgagcggttc
     3781 cattgcataa tcacatcaaa attcgggaat accatgtaag cagggccatc atagccctgt
     3841 ggcaacagaa ttgctgcttg aggcaaattc agtgcactgt ccggcacccc agccacgcct
     3901 gcttttaccc actcactcac aggtttattc aactgccatt gtgcctgact gtaatcaaac
     3961 ccagcaggca atgccacttg aagactaacc ggcttgccag gttgccagcc tgcttgcgcc
     4021 aggtaattgg ctgccgagct aaaaatatcc gggtaagagc cccaaatatc aatcttgcca
     4081 tcagcatcgg catcgacacc atatttgaga agagtagtcg gcatgaattg catgtgaccc
     4141 gtcgcgcctg cccatgagcc gaccaccgga ctatcgtagc gttgctcccg ctccactacg
     4201 cgcatcaggt taaacaactc acgtttaaaa aactcggcac gccgaccttc ataagagagc
     4261 gtagccaaag ccgaagccag tgggatatct cccataaatc cgccaaagtt ggtttccagc
     4321 ccccaaaaag ccaccaacac ttccctgggc acctggtata actcttcgac cacatagagc
     4381 accccttgat aatcctgcat cgcttgcttg cctttgctga cgacccgggg attgatacga
     4441 cgattcacat aggcagaaaa gctggtcacg aactcaggct gcttgcgatc caactcgatc
     4501 acgcggggca ggtaagtgac ctgattcagt gtggcatcta ccgcagccgg cgcaatctgc
     4561 aaggccaatg cctcctggcg caccgtcaca agccaggact ggaattgctc ttcatcatcg
     4621 gcgtaactca cgtgcgtgac aacggccaag ccaaccaaga atgcattccg gacagttgcc
     4681 caccactgag ctgtcttttt cttactcgtc ataaccctac tttaagttag ataaaatgga
     4741 tgcatagccc gcccgatagt cggcaaattg cagcttgaat ccactttgtt gtaaaaaccg
     4801 gttacggata cgtttaccgg actgcggcaa taatggcggc gcttctggtc caggcacttg
     4861 cttctgtgtg gctatccatt gcaagacttc atgttgtgca gcaggcaccc cgtcggagag
     4921 gatgcaatgc gcgggcaacg cctgtccctt agcaacttgc tggtacaaat atacgactgc
     4981 cgccgccaca tccaattcat gtatgcgatt ggtccagtgt gtttgtgttg gccaacgctc
     5041 cctgttttgc agcaagcgca acaggtataa gcgagttgcc ccataaatac ctgacactct
     5101 taatgcggta tgcccacacg tcaaaccttc cagcaactgc tccgcagcca gcatgatgtg
     5161 cccgctatca tctactggtt ttgcaacggt ttcctcatca atccactcac cttgatgctc
     5221 accataaatc ccggtgctgg aaacaaagaa tacgtgattc agggtatcct gttgagcact
     5281 tgccaagaca tgacgcaaac cgtccacata ggtttcctgg taactctggc cgtcgacggg
     5341 agccaggcag cacaaaagaa tatcggcttg cacattcgcc agggcagaca atccctcccc
     5401 agtcatcaca ttggcatata gcacttgcgc cccttccggc acaggctgat gtgaacgccg
     5461 gacgatcgtc aacgcatggc cttgcgccag cgtcgcttca gcaatcagac ggcccaatcc
     5521 accacatcca atttgcaata cccgcatacc catcgattac gaatctacac ctgagatggg
     5581 atgcttacgt agatagcgct cggccagcaa catcgccgac atggttttgc tatcagtgat
     5641 ttctcccgcc tcaatcgctt gcatcacttg ctcaaacggc cagacaaaga catccatggc
     5701 ttcatcaata tctctggcat acgcccctaa tgacaagcca gtcgcgagat agatatgaat
     5761 gacttcattg gagtagccga tacaggggtg ttgtacgcct aatttgaccc attcctgcgc
     5821 cgtgtaaccc gtctcttcaa gtaattcccg ttggccggtg gtcaaaaccg gttcatccgg
     5881 gtcaatttta cccgccggca actcaataaa cacctgcttc agtggataac gaaactgacg
     5941 ttccaatacc actccacctt cctgcgttaa tgcaatcaca accactgcac ccggatggat
     6001 cacatattcg cggccactga tgacgccgtt aggcaaacgg gcttcgtcgt aacgcacggt
     6061 cagcattttg ccctttgcca aaatctcgct tgatacggtc gtttcgatta aatgctgatc
     6121 ctgattgact gttttacttt ctgacatact gctatttcac cacacctaaa ctgaattttt
     6181 gaacgtgttg caatcattga cccgccctga ctgcagtcct tgcttaaacc aacgcacacg
     6241 ttgcgccgag ctgccatggg taaagctatc agggactaca tagccttgtg cctgcttttg
     6301 caacgcatcg tcgccaatgg ccgttgcagc gcgcaaggct tcttcaatat cgccctcttc
     6361 cagaatgcgg aattgctgat cggcatgatg cgcccagacc ccagcataac aatccgcctg
     6421 taattccaac ctgaccgagt aaccattcga ctcagcttcc gaacgtgcct gctgtctggc
     6481 ttgctgtacc ttagccgagg tgcccagcaa attctgtaca tgatggccga cttcatgcgc
     6541 aatcacatac gctt
//
LOCUS       DATMSK010000192         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_297061,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000192 DATMSK010000000
VERSION     DATMSK010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297061"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1754)
                     /locus_tag="VNZ84_06350"
     CDS             complement(273..1754)
                     /locus_tag="VNZ84_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWU66614.1"
                     /translation="MLLRHPLVVLTFTSVLTGCAVGPDYKKPDVTLTDHYLASAQTEQ
                     RSAVIYANLDRWWEGFNDPLLTRFVTIAFNQNLDLAQAAARISQARSGLSMTNVALLP
                     SGTMSASAARNHQSLETPLGQLLNATPGYDRNGRQYEANMSASWEMDLFGGLRRDQEA
                     AIAEYQASEAGRNATRLAVAANTADLYILIRSLQNRIAIAQHQLDIQQKLVTLVDLLH
                     GRGLAPTRELDLAKGARAQTQSTIPVLEASLDEAMNALDVMLGVPPGTYRNELATVQD
                     IPSSPQITSMGTPSDLLRRRPDLIMAERRLAASNARIGPAIAEYYPKFSITALLGTAT
                     AISSGNIFSSGANQATGLLGLRWRLFDFGRINAQIDLAKGQEAEAMAAYRQTALRATE
                     DVENALSSLIKREEQTSLLLQSENSLSQARNSSFLAYQKGAVSLIEVLHADENLFRTS
                     DAKAQAQTEAARSAVSAFKALGGGWEASSSSHQALSALSMNTD"
     gene            1840..2457
                     /locus_tag="VNZ84_06355"
     CDS             1840..2457
                     /locus_tag="VNZ84_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU66615.1"
                     /translation="MNKSTSPLTPPRGPVDHNVRTQIIEAADQHFSHYGYEKTTVSDL
                     AKSIGFSKAYIYKFFDSKQAIGEVICTNCLETIIANVKEDVAKAPTPSAKLRSIFSGL
                     VKNGIELFFQDRKLYEIAAFAAGEQWPPTLVYEDEIKQMLLNIIREGREAGEFERKTP
                     IDETVNAIHLVMRPFINPLLLQHNLHLVDDAPSLLSALVLRSLSP"
     gene            2532..>3439
                     /locus_tag="VNZ84_06360"
     CDS             2532..>3439
                     /locus_tag="VNZ84_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU66616.1"
                     /translation="MGRSQLASLAVFGFLILALSGCAEKPSPDPRTQLALVRVGSVEA
                     APSPSRAFTGTVSARVQSDLGFRVSGKVLARLVDTGQFVKVGQPLMRIDPNDFQLETR
                     ARVEAVAAARALADQTAEDELRNRDLVAAGAVSASAYDKIKAAADSAKAQLNAAEAQA
                     GVASNAFSYAVLVADADGVVVETLAEPGQVVAAGQPVIRVARAGQREDVVHLPETLRP
                     ALGSSAQASLYGRDATVSAKLRQLSGVADAVSRTFEARYVLEGELADAPLGATVTIRI
                     SENGAAHQNRFQVPVGAIFDAGKGPGV"
BASE COUNT          835 a          841 c          897 g          866 t
ORIGIN      
        1 gtgcaaaaga cggatcttca aaagctgaac tagaatcttc atgcaaattt gctttgaaag
       61 cgttgacatt ggttctacgc aactcccacg caaggtcgta ggtgatggta tctaccaaat
      121 gggattctac tggcgaataa tctttcattg acaatttagg catgcatctt ggactgataa
      181 aagatgggcg agcatgccaa ttgaacttgg agttgcgatc tgcctgaaag ctaacatata
      241 gttaatcacg aaaatctcag tttatctttc gactaatcag tattcatgga aagtgcgcta
      301 agtgcttgat gtgaagaact gctggcttcc cagccacctc ccagggcttt gaatgcactg
      361 acagccgagc gagcggcctc ggtttgggct tgcgcttttg catcggatgt tcggaataaa
      421 ttttcatccg catgcagaac ttcaatcaag cttactgccc ctttctgata cgctaaaaaa
      481 gaggaattcc gtgcttgtga gagtgagttc tcgctctgca ataaaagcga agtttgttct
      541 tcacgtttga ttaaagagga gagggcattc tcaacatcct ctgtcgcacg aagagcagtc
      601 tgacggtatg ccgccattgc ttcagcttct tgtcctttgg caagatctat ctgtgcattg
      661 atgcgtccaa aatcaaacag gcgccagcga aggccaagta gacctgtggc ttgattggcc
      721 ccgctgctaa agatattgcc gcttgaaatc gcagtagccg tacccagtaa agccgtgatg
      781 gaaaacttgg ggtaatactc cgcaattgct gggccaattc tcgcattgct ggcagccaag
      841 cggcgctcgg ccataatgag atcaggtcgg cgtctcagca gatcggatgg tgtacccatg
      901 gaggtgatct gtggggaaga tggaatatcc tgaacggtag caagctcatt cctgtaagtc
      961 cccggtggaa cgccaagcat cacatcaaga gcattcatcg cctcatcaag acttgcttct
     1021 agcacaggga tggtggattg ggtctgggca cgcgcaccct tagccaaatc aagctctctg
     1081 gtaggagcga gccctctgcc atgaagtaaa tctaccagcg tgaccaattt ttgctggatg
     1141 tcgagttgat gttgtgcaat ggcaatgcgg ttctgcaagc tgcgtatcag gatatacaag
     1201 tcggccgtgt ttgccgcaac cgccagacgc gtggcatttc tacccgcttc tgaagcctgg
     1261 tattcggcga tggcagcttc ctggtcacgg cgtagcccac cgaataaatc catctcccaa
     1321 gaggcgctca tattggcttc gtattgccga ccatttctgt catagcctgg ggtcgcattc
     1381 aggagttgtc ccaatggcgt ttcgagcgat tgatgatttc tggctgcaga tgcactcatc
     1441 gtgcctgacg gcagcaaggc gacgttcgtc atgcttaacc cggaccgagc ctggctaata
     1501 cgggcggctg cctgcgccaa gtctagattc tgattaaaag cgatggtaac gaaacgtgtt
     1561 agcaagggat cattgaaccc ttcccaccat ctatcaaggt tcgcgtaaat tacagcggag
     1621 cgttgttcag tttgtgcgga agccagataa tgatcggtta gcgtgacatc cggtttttta
     1681 taatcagggc caaccgcaca accagtcaat acgctggtaa atgttaaaac aaccagaggg
     1741 tgacgaagca gcataagatt ctcacttgtc tatatttgtg accatattat catttggtca
     1801 caagttgtca aatagtagaa atataggtac cgtgtttgta tgaacaaatc cacttctcca
     1861 cttactcctc cacgaggtcc ggttgatcat aatgtgcgta cgcaaattat cgaagccgca
     1921 gatcagcact tcagtcatta cggctacgaa aaaacaaccg tatctgatct ggcaaaatcc
     1981 atcggttttt ccaaggcata catctataaa ttttttgatt cgaaacaagc cataggtgag
     2041 gttatctgca ccaattgcct ggagaccatc attgccaatg tgaaagagga tgttgccaag
     2101 gccccgactc cttctgcaaa attacgaagc attttcagtg gcttggttaa aaatggtatt
     2161 gaactctttt tccaggatcg caagctgtat gagattgctg cctttgcagc gggcgaacag
     2221 tggccaccca cgcttgtgta tgaagacgag ataaaacaaa tgcttttgaa catcattcgg
     2281 gaaggtcgcg aggccgggga gtttgaacgc aaaacaccga ttgatgagac ggtaaatgcc
     2341 atacaccttg taatgcggcc attcataaac cctttgcttc ttcagcacaa tctacacttg
     2401 gttgacgatg ctccttcgtt attgtcggcc ttggttctca ggagcttgtc accataaatg
     2461 atttgtgact gttgactaat ttggtcactt aatcaacaat gagtccccat cactatatgg
     2521 aggggatact catgggacgt tcacagcttg cctcacttgc tgtttttggt tttttgatac
     2581 tggcattgtc agggtgcgcc gaaaagccca gcccggatcc acgtacacag ctcgcccttg
     2641 tcagggttgg aagtgtcgag gcagcacctt ccccttctcg tgccttcacg ggaacggtgt
     2701 ccgcacgcgt gcaaagtgat ctcggttttc gtgtctccgg aaaggtcctg gcgcgattgg
     2761 tggatacggg tcaattcgtc aaggttggcc aaccgcttat gcgcatagat cccaatgact
     2821 tccagttgga aacgcgtgcg cgcgtagaag cggttgccgc tgccagagcg ttggccgatc
     2881 agactgcaga agatgagctt agaaatcgcg acttggtagc ggcaggcgcc gtttctgctt
     2941 ccgcctacga taaaatcaaa gccgccgccg actctgccaa agcgcaactc aatgcggcag
     3001 aagcgcaagc gggcgtcgcc agcaatgcat tcagctatgc cgtgttggtg gcggatgcgg
     3061 atggtgtggt ggtggagaca ttggctgagc ctggtcaggt cgtcgcagct ggtcagcctg
     3121 tgataagagt tgcccgggcc ggacaacggg aggatgtggt gcacttgcct gaaaccctgc
     3181 gcccagcgct tgggtctagc gctcaggcca gtctgtatgg aagagatgcc acggtatccg
     3241 caaaactgcg ccagctgtca ggcgtagctg atgctgtttc ccgtacattt gaagccagat
     3301 acgtgctgga gggggaactg gccgacgccc cgcttggcgc cacggtcacc atccgtattt
     3361 cggagaacgg cgccgcacat caaaacagat ttcaagttcc tgtaggagcc atttttgatg
     3421 ccggcaaagg tccaggcgt
//
LOCUS       DATMSK010000193         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_14646,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000193 DATMSK010000000
VERSION     DATMSK010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14646"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..2048
                     /locus_tag="VNZ84_06365"
     CDS             240..2048
                     /locus_tag="VNZ84_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWU66617.1"
                     /translation="MIYAAPQLEVEAILAQKNLLPLFQPIVDIKQARIFAHEALIRGP
                     VESKLHSPIALFNAASVCGLNIEMEYASREVIFNEYSRTERAHPLFVNFSPDCLLLTD
                     SHLSFGLTQLARSGLKPDDIVIEITESSTIRDYAELRDAIARLKKMGFKIALDDLGEG
                     TSSLRLWSELSPDYVKIDKHFIANIHNDPIKLEFVRGIQKIAVESNTLTIAEGIETKE
                     ELAVIKDLKVDFAQGYLFGRPFPKFQYALTEEVENLLNKNIIRLFADHQLASSKQATV
                     ISLASYQTAAFPQMTNEEVYNWFDRENTLNSIPVIDHKSKPLGLISRYDTIDRFARRY
                     QKELHGKKSCTTFMDANCLVVQKDMSILAMSELILAADPKYLLNGFIIADGERYVGMG
                     SGHALLREVTNMQIHAARYANPLTLLPGNVPINAHIDKLLERRIPFVACYCDLDHFKP
                     FNDAYGYRRGDEVIQFVGRLLSSLVNHEQDFVGHIGGDDFVLVFQSERWEVTCKDILD
                     TIVKVMPDFYDLKDIQQGGIRIEDRLGNEHFFPFGSLSIGAVQVHAEAFASHHEVAGA
                     MSNAKKQAKKILGNSLFIERRRLATTANRAMDESIS"
     gene            complement(2045..2830)
                     /locus_tag="VNZ84_06370"
     CDS             complement(2045..2830)
                     /locus_tag="VNZ84_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HWU66618.1"
                     /translation="MTETGSQRGMRWVKESLALFRMQPHVWMLCSLAYIVIFMVLPSL
                     PLLGFLGLISVVIWPVCMAFVVMLYRQMDLQGSMSPRTVWQQLQQHFKPLVLLGASCL
                     IYAVLATYLLSSELASLVSHAPTKVGMTESQMTQFLENTLSFIVKVMLLLLPLFIATW
                     FSPMLIALNGYPLLKAIKSSIAGMLQYTVPMGVSWLVMTAGMLVVMLGLGLVIGILGA
                     LIPMLAQLLMPLLVFAALLVINALMFAFQYISYKDVFRAAPQV"
     gene            complement(2856..3782)
                     /locus_tag="VNZ84_06375"
     CDS             complement(2856..3782)
                     /locus_tag="VNZ84_06375"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWU66619.1"
                     /translation="MSVFTTLSLEEVRQWLAPFNVGELQTLRGIAAGITNTNYFVETS
                     ESRYVLTVFEKNDFDELPYFVHLMTHLSRHGIPCPKPVTDENHIALHRIHGKPALMVS
                     CLKGSDVSQPNVKQIEAVAWTLARMHVAGLDFHEQSHNQRGQGWRVMTAQQVMPKLAP
                     VQQHLLQEELEFQHGLDLSRLPHGVIHGDLFRDNVLFDGDALGGFIDFYYACHDVLAY
                     DVAIAANEWCVDANGNFVDEKLAAFMNAYQSERPFTEAEKTHWPALLRRAALRFWLSR
                     LYDFYYPVAGELTHAKDPAHFERILLNRKVLT"
     gene            complement(3821..4165)
                     /locus_tag="VNZ84_06380"
     CDS             complement(3821..4165)
                     /locus_tag="VNZ84_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HWU66620.1"
                     /translation="MRNFSRLLLAGICFAAIAMPAWAAAPNDAELLEEVPSPPKVIEG
                     QPIDQPTVNKRKDGENDIEEYSLNGEVYMVKVTPPSGKSYYLHREDRNGDWVHDGPTR
                     PLSVPKWILFRF"
BASE COUNT         1163 a         1150 c         1035 g          988 t
ORIGIN      
        1 ccattcagca tttgaaatgt ctgctgcaag tttctaatgg agatttgcgt cagaatatac
       61 agcagttgcc atcagatcac gccagactgc ttcaacgttt gttagccgaa atcaaacaaa
      121 acaagcccat ccagtagtgc agaattcagt cttgaatttt cctggccctg tcacaaataa
      181 ttcatcccct ctttgcacaa cttaatcaag atgccgccaa aacagtcgat actgctcgca
      241 tgatctatgc cgctccccag ctcgaagttg aagccattct ggcgcaaaaa aacctgttac
      301 cgctgtttca accgattgtc gacattaaac aggcccgcat ttttgcacat gaggcattga
      361 tacgcggccc ggttgaatcc aaactgcatt ctccaattgc gctctttaat gcggccagtg
      421 tttgcggttt gaatatagag atggagtatg cctcgcgtga agtgattttt aatgagtaca
      481 gccgcactga gcgagcacac cccctatttg tgaactttag cccagactgc ctgctactga
      541 ctgacagcca tttgtctttt gggttaaccc agctcgcgcg cagtggactg aaaccagacg
      601 atattgtgat tgaaatcacg gagagctcga ctatccgcga ctatgccgaa ctccgtgacg
      661 cgattgcccg actgaaaaag atggggttca aaattgcgct ggatgatctt ggcgaaggga
      721 cctccagttt gcgcctgtgg tccgaactat cgccagacta tgtcaaaatc gacaagcatt
      781 ttattgccaa tatccacaac gacccgatca agctggagtt tgtacgaggc atacaaaaaa
      841 tcgccgttga atccaacacg ctcaccatcg ccgaagggat agaaaccaaa gaagaactgg
      901 cggtgattaa agacctcaaa gtggattttg cgcaaggcta cctgtttggt cggccatttc
      961 ccaagtttca gtatgccttg actgaggagg tcgagaacct gctcaataag aacattatcc
     1021 gcttgtttgc cgaccatcaa cttgcatcct caaaacaggc cacagtcatc agtcttgcca
     1081 gctaccagac ggccgctttt ccgcagatga cgaacgaaga ggtgtataac tggttcgacc
     1141 gcgaaaatac gctcaactcg attccggtca ttgaccataa aagcaaacca ctaggcttga
     1201 tcagccgtta cgacaccata gaccgctttg cccggcgtta ccaaaaggag ttacatggca
     1261 aaaagtcctg tacaacgttc atggacgcca actgcctggt ggtacaaaaa gacatgtcga
     1321 ttctggcaat gagcgagctc attttggcag cagaccctaa atacttactc aatggattca
     1381 tcattgccga cggtgaacgc tatgtgggca tgggcagcgg gcacgcacta ctgcgcgaag
     1441 tcaccaatat gcagattcat gcagcacgct atgctaaccc gctcaccctg ctccctggca
     1501 atgtcccgat caacgcccac attgataaac tactagagcg ccgcattcct tttgttgcct
     1561 gctattgcga tctggaccac ttcaaaccct ttaatgatgc ctatggttac cggcgtggtg
     1621 atgaggtcat ccagtttgtc ggccgtttac ttagcagcct ggtcaaccat gagcaggatt
     1681 ttgtgggaca tataggcggc gacgactttg tcttggtgtt tcagagcgag cgctgggaag
     1741 tgacttgcaa ggacatcctg gataccatcg tgaaagtcat gcctgatttt tacgacctca
     1801 aggatattca gcaagggggt atccgcatag aggaccgctt gggaaacgag cactttttcc
     1861 cgtttggcag cctatccata ggcgcagtac aggtacatgc cgaagctttt gccagccatc
     1921 atgaagtggc tggcgcgatg agcaatgcca aaaagcaggc caagaaaatc ctgggcaaca
     1981 gcctctttat agaacgccgg cgattggcaa caacggccaa ccgggcgatg gatgagtcta
     2041 tttcctagac ttgtggtgca gccctgaaga catccttgta gctgatatac tggaaggcaa
     2101 acatcaaggc attaatcacc agcaaggctg caaacaccag tagtggcatc agcaattgtg
     2161 ccaacatcgg gatcaaggca cccaaaatgc caatcaccaa gccaagccct aacatcacga
     2221 ccaacatgcc tgccgtcatc accagccatg aaacgcccat gggcacggtg tattgcaaca
     2281 tacccgcaat gctagattta atcgccttga gcagcgggta gccattcagc gcgatcagca
     2341 tgggcgaaaa ccaagtggca ataaacagtg gcaacaacag caacatgacc ttgacgataa
     2401 atgacagcgt gttttccaga aactgcgtca tttgggactc ggtcatcccc actttggttg
     2461 gcgcatgtga aaccagcgag gcaagctcac tgctgagcaa gtaggtggct aacaccgcat
     2521 agatcaggca actagcgccc aacaacacca atggtttgaa gtgctgctgt aattgctgcc
     2581 acaccgtacg cggcgacatg ctaccttgca aatccatctg gcgatataac atgacaacaa
     2641 acgccataca gaccggccag atcacgacag aaatcaggcc caggaagccg agcaagggca
     2701 aggacggcag caccatgaaa atcacgatat aggccagcga acacagcatc cacacatgcg
     2761 gctgcattcg gaacaaggcc aggctttctt tcacccagcg catgccgcgc tggcttccgg
     2821 tttctgtcat gtgtctccct ttttatctct taatattaag tcaaaacttt acgatttagc
     2881 agtatccgct caaaatgcgc cggatcttta gcatgggtca actcgcctgc aaccggataa
     2941 taaaaatcat acaggcggga gagccagaaa cgcaaggcag cccggcgcaa caatgcgggc
     3001 caatgcgttt tttcggcttc ggtaaatggg cgttcagact ggtaggcatt catgaatgcg
     3061 gccagttttt cgtcgacgaa gttaccattc gcatcgacac accattcgtt cgcagcaatc
     3121 gccacatcat aggccagcac atcgtggcag gcatagtaaa aatcaataaa cccaccgagc
     3181 gcatcgccat caaacaacac attgtcgcga aacaggtcac catgaatgac cccatgaggc
     3241 aaccgcgaca gatccaggcc atgctgaaac tccaattcct cttgcaacag atgctgctgc
     3301 actggtgcca gcttgggcat cacttgttgg gcagtcatca cccgccagcc ctgcccacgt
     3361 tggttgtggg actgttcatg aaaatccagt ccggccacat gcatgcgtgc caaggtccag
     3421 gcgacggcct caatctgttt tacgttcggc tgactgacat cgctcccttt caggcaactc
     3481 accatcaggg cgggttttcc gtggatgcgg tgcaaagcga tgtgattctc atccgtcact
     3541 ggtttggggc aaggaatgcc gtgccgggaa agatgtgtca ttaaatggac aaaataaggc
     3601 agttcatcaa aatcattttt ttcaaacaca gtcagcacat agcgggattc gctggtttca
     3661 acaaaataat tggtattagt aatcccggcg gcaataccgc gcagggtttg caactcgcca
     3721 acattaaaag gcgccagcca ttggcgcact tcttccaaac tgagggtggt aaagacagac
     3781 atattgatta gctagcctgc cggaaggcag gccttcaaga ttagaatcgg aacaggatcc
     3841 atttaggaac ggatagcggg cgtgtcgggc catcatggac ccaatcacca ttgcggtctt
     3901 cgcggtgtag ataataggac ttgccactag gtggcgtcac cttcaccata tacacttcgc
     3961 cattcaggct atattcctca atgtcatttt cgccatcttt gcgtttgttc actgtcggct
     4021 gatcaatggg ttgcccttca atcacctttg gcggtgacgg cacctcttct aatagctcgg
     4081 catcgttggg ggcagctgcc cacgctggca ttgcgatcgc agcaaagcag ataccggcca
     4141 gtaacaatcg ggaaaaattg cgcatgaagg actccttagt cagtgacgcc tattctagca
     4201 aaaaccaccc tgctggcggt aactgcaaac agaataaact taaataaagc ggggcaactt
     4261 tcacccggaa tgagtcaatt agaggtagag ctgcattttg cgttctgccg gggtcagctc
     4321 aaagccagtc gtttca
//
LOCUS       DATMSK010000194         6023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_297127,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000194 DATMSK010000000
VERSION     DATMSK010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297127"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="sbmA"
                     /locus_tag="VNZ84_06385"
     CDS             <1..1018
                     /gene="sbmA"
                     /locus_tag="VNZ84_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HWU66621.1"
                     /translation="AVFAVFWFKLKPTRWQWWSIVGSAFILFSTYYSVQVSVAINNWR
                     RPFFDGVQAALSPNSKVTTAELYTLMLQFAEIAFVAIFIYVITRFFVSHYIFRWRTAM
                     NDFYVSRWPQVRRIEGASQRVQEDTMRFATIMEGLGVSMVEAIMTLVAFLPVLWSLSS
                     YVSELPVFGAVPAPLFTSAIAWSVFGTLLLAAVGVKLPGLEFRNQRVEAAYRKELVYG
                     EDNAERAQPITLAALFMDVRKNYFRLYFHYMYFNVARSLYLQADNIFAYLILVPTIAA
                     GKITFGILQQILTAFSQVSNSFQYLVNSWTTIVELLSVYKRLTAFEAAIKHEALPEIE
                     QTVS"
     gene            complement(1039..3204)
                     /locus_tag="VNZ84_06390"
     CDS             complement(1039..3204)
                     /locus_tag="VNZ84_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU66622.1"
                     /translation="MNSHTTLADLMQNDIMESLLTAQQQRLLDTLLDNLDGMIYRCLY
                     DSNWTMIYISKGCEPLTGYPAQELLLNKTISYENITHPDDREKARATIAAAIEKGERF
                     ELEYRIVRADGEEVWVAERGSAVLDPSGQPVALEGIIQDISSRKRIEHELFSTEQKYR
                     SMFENSTLGMFQTTESGTYLNANPALAKLYGYASPAALISDLNNISSQLYVAESRRGE
                     FTEAIQAKGRVLNFESEVYRLNGETIWISENAHAVYDAQGNILYYEGTVEDITDRKNY
                     ELQIRQLAVTDNLTGLLNRHAFSGKLASLIADADKMQRKIAIAFIDLDHFKLINDTLG
                     HRAGDKLLENVAQRLIQSTRPTDAIVRFGGDEFVMLYPGLRSSEDAEPLLARVMEAIS
                     QPIAIGDYVFNMTCSVGVSIYPDHAQEIDTLLTCADSALYSAKNAGKNKVQMFSGTLA
                     QQLGERNEIEYGLGHALQRQELMLYYQPQINMQHGNISALEALIRWQHPEKGLIPPDQ
                     FIPVAEETGHILAIGEWVLQTACQKIKSMQTEWGYLVPIAINVSPIQFLRGNLVETVQ
                     RVLRQERVPPHLITLEVTENARFKDEAMFTRTLEQLKRLGVCIAIDDFGIGYSNMAYL
                     KNYPIDELKIDKAFVQDLEQSVTNGNILLALINLGKSLHKSVTAEGIETEYQHRFLLH
                     SGCDVGQGFYYSRPLSEEALAQFMRDQRPKFDHLQQVAR"
     gene            complement(3204..5786)
                     /locus_tag="VNZ84_06395"
     CDS             complement(3204..5786)
                     /locus_tag="VNZ84_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWU66623.1"
                     /translation="MHPQPLTEDLANHFKKVNDFRTHIYQLQHAWDSLALMAQLSGTG
                     SEMEETRSAFNTISNDVLRHLSQETLHKTAAQLSSKAFEIINLLVRNLFERTADIGFF
                     ATDDDVREYLEMQAADTLDADDLHRLEYRFQEYQKKYTVYENIVLFDAQGHMLHQLDD
                     TQTMLNHYHPIVELAKQSTEPYVERFYETEINGRMRKSLVYAHEVRSKDESRSLGVVC
                     LCFKFVNELDTIFHNLISHNDWTIGVLLDEERNVIASSDPYQIPLGINLCVDTDTDWF
                     ITRFSGREYLCVTKQSEGYQGYLGPGWLGQAMIPIEHAFNQSIHDIESSIDAETLRKV
                     MRSPLIFSESLLNIPKQAANIQSKLNQSVWNGNIWQSNYVNTQQKNFSKSLLSEISHT
                     GHKTQKIIENMVSELYQTVVSVMLENSSFYAQLAVEIMDRNLYERANDVRWWALTHVF
                     RELLAKASLADEDKQKMTAVLSYINSLYTVYDNLIVFDRKGEVIAVSNTQYAHLIGQP
                     LADEWVGRTRSCASTQDYVVSQFEPTPLYNDKPTYIYAAPIRHLDGNSIVGGIAIVFD
                     STPQFQAMLRDVIPRDKTDTPVSGSFTLFVNEQLSVIASTHRDFTIGDTFELMPAITK
                     LKEGDQLFDIAVYQNTYYAVGARAAYGYREFKSEQDSYRNKVVALIFTPLGKVDEINQ
                     RILAEEQVIHNKFNPSLFAQSGQDSQEYATFYVGDSWLGIEARLVREAIDEEQLRTLP
                     ESAPFIAGMHTYRDGVIPVIDLARMLGHPHYFTDYRQIVVIEDQASSLKFGIFVSALG
                     EIPYLSASQIQPMNTFFKGASNNPGIAIANLAQNSMLTLITASSLWEKVLSVRSNPAE
                     QVYA"
BASE COUNT         1383 a         1460 c         1541 g         1639 t
ORIGIN      
        1 ggctgtattt gcggtgttct ggttcaagct gaaaccgacg cgttggcaat ggtggtcgat
       61 tgtgggttca gcatttatcc tgttttcaac ctattattcc gtgcaagttt cggtcgctat
      121 caacaattgg cgccggccat tctttgatgg tgtgcaggca gcgctgtcgc caaactccaa
      181 agtgacaacc gctgaactgt acactctcat gttgcagttt gcagaaattg cattcgtggc
      241 gatttttatt tatgtcatta cacgcttttt tgtcagccac tatattttcc gctggcgtac
      301 ggccatgaac gatttttacg tgagccggtg gccacaggta cgcaggatag agggtgcttc
      361 acagcgggtg caggaagaca ccatgcgctt tgcgacgatt atggaaggct tgggtgtgtc
      421 catggtggaa gccatcatga cgctggtggc tttcctgccg gtgttatgga gcttgtcttc
      481 ctacgtgagt gagttgccgg tatttggtgc tgtgcctgcg cctttgttta catcggcgat
      541 tgcatggtca gtctttggca cattattgct ggcggcggtc ggtgtgaaat tgcccgggct
      601 ggaattcagg aaccagcggg tagaagcggc gtatcgtaaa gagctcgttt atggcgaaga
      661 caacgccgag cgggcgcaac cgatcacact ggcggcgctg ttcatggatg tacgcaagaa
      721 ttatttccga ctgtatttcc actatatgta ctttaatgtg gcgcgcagtt tatatttgca
      781 agcagataat atttttgcct atctgatcct ggtacccacc attgctgccg gtaaaatcac
      841 ttttggcatc ctgcagcaaa tcctgactgc attttcacag gtcagtaatt cgttccaata
      901 cctggtgaat tcctggacga ccattgttga gttactgtcg gtatataaac ggttgacggc
      961 atttgaggct gccatcaagc atgaagccct gcctgaaatc gagcagacag tttcctgata
     1021 aaacagcagt ggccagcgtt acctggccac ctgctgcaag tgatcgaatt taggccgctg
     1081 gtcacgcata aattgcgcca gcgcttcctc tgataacggt cggctgtagt aaaagccttg
     1141 gccgacatcg cagccgctgt gtagcaaaaa gcggtgctgg tattccgttt ctatcccctc
     1201 ggccgtcact gacttatgca ggcttttgcc taggttgatc aatgccagca ggatgttgcc
     1261 gttggtcaca ctttgttcca ggtcttgcac aaatgccttg tcgattttta attcatcgat
     1321 ggggtagttt ttgaggtagg ccatattgga gtagccgata ccaaaatcat caatggcaat
     1381 acacacgcct aaccgtttca attgctccag ggtgcgtgtg aacatggctt catccttgaa
     1441 gcgcgcattt tccgtgactt ccagcgtgat caggtgtggc ggtacgcgct cctgccgcaa
     1501 tactcgttgc actgtttcca ccaggttgcc acgcaaaaac tggatgggcg agacattgat
     1561 ggcaattggt accaggtagc cccattcagt ctgcatggat ttgattttct gacaggcggt
     1621 ttgcagcacc cactcaccaa tggcaagaat atggccggtt tcttcggcca ccgggataaa
     1681 ctggtcgggc ggaatcaatc ccttctctgg atgttgccag cggatcaggg cttccagagc
     1741 actgatattg ccatgctgca tattgatttg cggctggtaa tacagcatca gctcctgccg
     1801 ttgtaatgca tggcccaggc cgtattcaat ttcattgcgc tcacctaact gctgtgccaa
     1861 tgtgccacta aacatctgta ccttgttttt accggcattt ttggcactat acaacgccga
     1921 atctgcacag gtgagcaggg tgtctatctc ctgcgcatgg tccgggtaaa tactgacacc
     1981 cacactgcac gtcatgttaa agacatagtc gccaatcgca atcggttgtg aaatggcttc
     2041 catcacacgg gccagcaagg gctcggcatc ttctgaggaa cgtaagcccg ggtagagcat
     2101 gacgaattca tcgccaccga aacgcacaat tgcatcggtg ggtcgtgtgc tttgaatcaa
     2161 gcgttgtgcg acattttcca gcagtttgtc accggcacgg tgccccaagg tatcgttaat
     2221 cagcttaaag tgatcaagat caataaaggc aatcgcaatc ttgcgttgca tcttgtcagc
     2281 atcagcaatc aggctggcca gcttgccact aaacgcatgg cggttcagta agccggtcag
     2341 gttgtcggtc actgccagtt gccgaatctg caattcataa ttcttgcggt cggtaatatc
     2401 ttctacggtc ccctcgtaat agaggatatt cccttgcgcg tcataaaccg catgtgcatt
     2461 ttcagaaatc cagatggttt caccattcag gcggtatacc tcggattcga agttgagtac
     2521 gcgccctttg gcctggatgg cttctgtgaa ctcgccacga cggctttccg ctacatacag
     2581 ctgcgagctg atattattca ggtcactaat cagggcggca ggtgaagcat aaccatagag
     2641 cttggccaac gccggattgg cattcaggta agtgccagac tcggtagtct ggaacatgcc
     2701 cagtgtcgag ttctcgaaca tggaacggta tttttgttcg gtactaaata actcatgctc
     2761 aatgcgttta cggctgctga tgtcttgaat aattccttcg agcgcaactg gttgtccaga
     2821 aggatccagc acagcactgc cgcgctcggc gacccacacc tcttcaccat cggcgcggac
     2881 gatgcggtat tccagttcaa aacgctcacc tttttcgatg gccgcggcaa tcgtggcccg
     2941 tgctttttcc cggtcatccg gatgggtaat gttttcgtag gaaatggttt tgttcagcaa
     3001 taactcctgc gcagggtaac ctgtgagagg ctcacagccc ttgctgatgt aaatcatggt
     3061 ccagttgctg tcatacaggc agcggtagat catgccatcc agattgtcca gcagcgtatc
     3121 aagcaatctt tgttgctgtg ctgtcagtaa ggattccatg atgtcgtttt gcatgaggtc
     3181 agcgagtgtc gtatgactat tcatcaggcg taaacctgtt ctgccgggtt agaacggacg
     3241 gacagtacct tttcccacag gctgctggcg gtgatcaatg tcagcatgct gttctgcgca
     3301 agattggcaa tggcaatgcc cggattgttg ctggcgccct tgaaaaacgt attcatgggc
     3361 tggatctggc ttgccgacaa ataaggaatc tcacccaatg ccgagacaaa tatgccaaat
     3421 ttgagtgatg aagcctggtc ctcaatcacc acgatttgcc ggtaatcggt aaagtagtga
     3481 ggatgtccca gcatgcgcgc caggtcaatc accggaatca cgccatcacg gtaggtgtgc
     3541 atcccagcga tgaagggcgc tgactctggc agtgtgcgta gttgctcttc gtcaatggct
     3601 tcacgtacca aacgtgcctc tatccccagc caggagtcac ccacatagaa ggtggcgtac
     3661 tcttggctat cctgccctga ctgcgcgaac aggctagggt taaatttatt gtggatgact
     3721 tgctcctcgg ccaggatgcg ctggttaatt tcatccactt tacccaaggg ggtaaagatc
     3781 agggcgacca ccttgttgcg gtagctgtcc tgttcgctct tgaactcacg gtaaccataa
     3841 gctgcgcggg cccccacggc ataataggta ttttggtaaa ccgcaatgtc aaacagttgg
     3901 tcaccttctt tgagcttggt aatggctggc atgagctcaa acgtgtcgcc aatagtgaag
     3961 tcgcgatggg tgcttgcaat aacactgagt tgttcattca caaataaggt aaagctgcca
     4021 ctgacgggtg tatctgtttt gtcgcgcgga atcacatcac gcaacatggc ctggaattgc
     4081 ggggtgctat cgaacacaat cgcgatgcca ccaacgatgc tattgccatc caggtgacgg
     4141 ataggagcgg cgtaaatata ggtcggcttg tcattgtaga ggggagttgg ttcaaactgg
     4201 gaaaccacat aatcttgcgt cgaggcgcag ctacgcgttc gacctaccca ctcgtcggcc
     4261 aacggttgac caattaaatg cgcatattgc gtattggata ccgcgataac ttcgccttta
     4321 cggtcaaata cgatcaggtt gtcatagacg gtatacaggc tgttgatgta actcaacacg
     4381 gcagtcatct tttgtttgtc ttcatcagcc aggctggctt tagccaggag ctccctgaaa
     4441 acatgtgtga gtgcccacca acgtacgtcg ttagcgcgct cgtacaggtt gcggtccatg
     4501 atttccacgg ccagctgcgc ataaaaactg gaattctcca gcatgactga taccacggtc
     4561 tggtagagct cggacaccat gttctcaatg attttttgcg ttttgtgccc tgtatggctg
     4621 atctcggaaa gcaggctctt ggaaaagttt ttctgctggg tattcacata gttgctttgc
     4681 cagatattgc cgttccagac cgattggtta agtttgcttt gaatgttagc tgcctgtttg
     4741 ggaatgttga gcaggctttc agaaaaaatt agcgggctgc gcatcacctt gcgcagggtt
     4801 tccgcatcaa tgctgctttc aatgtcatgg atgctctggt tgaaggcatg ctcgatcgga
     4861 atcatggcct ggcccagcca gcccggtccc agatagccct ggtaaccttc tgactgtttg
     4921 gtgacgcaca ggtattcgcg accgctaaaa cgggtgatga accagtcggt atcggtatcc
     4981 acacagagat tgatgccgag cggaatctga tacgggtcac tggaggcaat cacattgcgc
     5041 tcttcatcca gcaatacgcc tatggtccaa tcattatggg aaatcaggtt gtggaaaatg
     5101 gtatccagtt cgttgacaaa tttaaagcac aggcaaacca cacccaagga gcggctttca
     5161 tccttgctgc gcacctcatg tgcatacacc agtgatttgc gcatccggcc attgatctct
     5221 gtttcataaa acctttccac ataaggttct gtggattgtt ttgccagttc cacaataggg
     5281 tggtaatggt tgagcatggt ttgggtatca tcgagctgat gcaacatatg gccttgcgca
     5341 tcaaacaaca caatgttttc gtagacggta tatttcttct ggtattcctg aaaacgatac
     5401 tccaggcgat gcagatcatc ggcatccagg gtgtctgcgg cttgcatttc cagatactcg
     5461 cgcacgtcat catcggttgc aaaaaaaccg atgtctgctg tccgttcaaa caggttgcgt
     5521 accagaaggt tgataatttc aaacgcttta gaagataact gtgcggcggt tttgtgcagg
     5581 gtttcctgcg acaggtggcg caatacatcg tttgagatag tgttaaatgc gctacgggtt
     5641 tcttccattt cagagcccgt gccagacagc tgggccatca gtgccaggct atcccaggca
     5701 tgttgcaatt gataaatgtg agtacgaaaa tcattgacct tcttaaagtg attcgctaag
     5761 tcctctgtta aaggttgcgg gtgcatctat tgcctctttc gctatgctaa atgggttgtt
     5821 gctagtgatt aacatgtgct ttacagcaag aatggttccc gttttggcag gttgaattgc
     5881 aaaacaatgg caagaaaaat gcttttatta caatattaaa gtcctaaatg cacccaacat
     5941 ggtgtaaaaa tcgcttatca atgattgaaa tagcaggtaa ctcccgtctt tttcgcggaa
     6001 tcgttttctc tgtgcatttt tac
//
LOCUS       DATMSK010000195         4872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_155449,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000195 DATMSK010000000
VERSION     DATMSK010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155449"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VNZ84_06400"
     CDS             <1..1468
                     /locus_tag="VNZ84_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HWU66624.1"
                     /translation="VHLQGEFRYLDENYSGINNIEYLDKDSMTDQTRYYVRLSHRQNF
                     SDHWSGGYNLEKVSDNTYFSEMATRIQVTSRVNLQQSAFVNYNSEHVTFNGTVQRYQN
                     LDNVSYVYQRLPQLTLSYNDEWKGFKTQTGAQYVNFQTMDGMINAPSGSRLSFYPSVS
                     YPMKNAYGYVTPKVGVQTTQYSLNNNIPLGYSQEYNQLNRTLPIVSLDSGMYFDRETS
                     LFDNTYTHTLEPQAYYVYIPYRDQSKIPIFDTSLNTLNQNSIFYENQFSGDDRINDAN
                     QMTLALTSRLIDSETGIERLSGTLGQRFYFEDQKVTLPTTTSSTRKSSDILAGFTARL
                     SSRLNLDTFWQYDPDRGNMQRNNYLLRYNPEPGKSFTFGYRYTANTLEQLDTSAQWPL
                     GTGWYGIGRLNYSIRDGNAVQFLGGLEYDAGCWQARAVMQRVQTATANANYAMFFQLE
                     LGGLTSIGANPLNVIKRNIPSYMRTYDIPTIYRDQNSQ"
     gene            1465..2781
                     /locus_tag="VNZ84_06405"
     CDS             1465..2781
                     /locus_tag="VNZ84_06405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWU66625.1"
                     /translation="MMKSLFNFLLSVVVLTLSATPVALSATEKPPIEKMDRIIAVVDQ
                     SVITERELMDRTESVKAQMIKKGVEVPPDEVLQKQILERLIVDNLQLQMAAQTGIKVD
                     DAQLDKTIERIAEQNNLTLPAFKKALEEEGTRFYKFREDIRNDIILARLRERDVDNKV
                     NISEAEIDNYLSTQEKEGDLDEFNISQILIRLPEDSSPEDIQKARARTEQVIKALSEG
                     MSFEKASANFSDASNALDGGTLGWRSGQQMPPEFLELIKTLQVGGVSRPFRSGNGVHI
                     LKLNERRAGSSTLIVEQTHVRHILLKPNEVLSDKEAKQKIEGIKERIDHGTPFQDMAR
                     QYSDDGSASNGGDLGWISPGDTVPVFEKTMAELAINEVSAPIRSQFGWHLIQVLERRK
                     QDMSKESKRLKARQEIRARKAEEAYNEWIHELRDKAFVELRLEDKF"
     gene            2785..3783
                     /gene="pdxA"
                     /locus_tag="VNZ84_06410"
     CDS             2785..3783
                     /gene="pdxA"
                     /locus_tag="VNZ84_06410"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWU66626.1"
                     /translation="MNHLPRIAITSGEPAGIGLDICVKLAHTPVPAALVVLADPNALR
                     ARASQLGLPLTITPYTEASIEHVGDGCLSVLAIETAAPVIAGELNKNNSTAVLTMLDR
                     ALSGCLADEFDAMVTAPVHKGIINDAGITFTGHTEYLAEKTNTPQVVMMLVGGGMRVA
                     LATTHLPLTQVSAAITASALTSTIRILHDDLVHKFGIAQPSIYVAGLNPHAGENGYLG
                     TEEIETINPVLDHLRAEGMQLTGALPADTMFSKKNLAHADAFLAMYHDQGLSVLKHAS
                     FGEGVNVTLGLPIIRTSVDHGTALDIAGSGQADVGSMLSAIQLAIDLSASRHTKHA"
     gene            3780..4553
                     /gene="rsmA"
                     /locus_tag="VNZ84_06415"
     CDS             3780..4553
                     /gene="rsmA"
                     /locus_tag="VNZ84_06415"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWU66627.1"
                     /translation="MKHIAKKKFGQNFLIDQGIIQSLINAIRPQEDDLMVEIGPGLGA
                     MTQPLIKHLNHLHVVEIDRDIIQWMQGFYPPKKITIHNSDVLKFDFATIGERIRVVGN
                     LPYNISSPILFKLLENTAQIIDMHFMLQKEVVERMVAAPSSAEYGRLSVMLQYRLQME
                     YLVTVPPEAFDPAPKVESAFVRCVPYATLPYPAKDEALFAKIVTAAFGQRRKTLRNTL
                     KGLLDDDGFTALNLDSQLRAENLGVAEFVAISNHLADNA"
     gene            4648..>4872
                     /locus_tag="VNZ84_06420"
     CDS             4648..>4872
                     /locus_tag="VNZ84_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HWU66628.1"
                     /translation="MRLILLGAPGAGKGTQATFIKEKFNIPQISTGDMLRAAVKAGTQ
                     LGLEAKKFMDAGGLVPDEVIIGLVKERIKDA"
BASE COUNT         1333 a         1258 c         1172 g         1109 t
ORIGIN      
        1 cgtgcactta caaggggaat tccgctacct tgatgaaaac tattccggga tcaataatat
       61 tgagtacctg gataaagaca gcatgaccga ccaaacccgc tattacgtca ggctgtcaca
      121 tcggcaaaat ttcagtgacc actggtctgg cggctataat ctggagaagg tgtctgataa
      181 cacttacttc tccgagatgg cgaccagaat tcaggtcacc tcgcgcgtca acctgcaaca
      241 aagtgcgttt gtaaactata acagtgagca tgtgacattc aatggtacgg tacagcgtta
      301 tcaaaacctg gacaatgttt cttatgttta ccaaagactg ccccaattga cgctgagtta
      361 taacgatgag tggaaagggt ttaaaaccca gacgggcgca caatatgtca atttccagac
      421 catggatggc atgatcaatg caccctcagg cagccgcctg agcttctatc cttctgtgtc
      481 ttaccccatg aagaatgctt acggctacgt cacacccaaa gtgggggttc aaacgacgca
      541 atacagcttg aacaataaca ttccgttagg ttattcccaa gaatataacc agcttaaccg
      601 gacactgccg attgtcagcc tggattcggg catgtatttt gaccgtgaaa ccagtttatt
      661 cgataacacc tacacgcata ccctggaacc gcaggcatac tatgtctata ttccctatcg
      721 tgaccagagc aagatcccga tttttgatac gtccctcaac acactgaacc agaactcgat
      781 tttctatgaa aaccagttta gtggcgatga cagaatcaac gatgccaacc aaatgacgct
      841 ggcattaacc agccgcctga ttgacagtga aaccggcatt gaacgcctga gcggtacgct
      901 tggacagcgc ttctattttg aagatcaaaa agtaacgctg cctaccacaa caagcagcac
      961 acgcaaatcg tctgatattc tggcaggatt tacagcgcgc ctttccagcc ggttgaacct
     1021 ggatacattc tggcaatatg acccagaccg cggcaatatg cagcgtaaca actatttgct
     1081 acgttacaac ccagagcctg gcaagtcatt cacatttggt taccgctaca cggcgaatac
     1141 gctggagcaa cttgatactt cagcacaatg gccattagga accggctggt atggcatcgg
     1201 cagactcaat tactctattc gtgacggcaa cgctgtgcaa ttcctcggcg gcctggaata
     1261 tgatgctgga tgctggcagg cgcgtgcggt catgcagcgc gtgcaaacag caacagccaa
     1321 cgcgaactat gccatgtttt ttcaactcga actgggtggg ctgacttcga ttggcgccaa
     1381 ccccttaaat gtgatcaagc gcaatattcc aagttatatg cgcacctacg acattccgac
     1441 aatttacaga gaccagaact cgcaatgatg aaaagcttat tcaatttctt gctatcagtc
     1501 gttgtgctta ccttaagtgc aacacctgta gcattatccg ctactgagaa accacccatt
     1561 gaaaaaatgg accggattat tgcggtcgtg gaccaatccg tgattacaga acgcgagctg
     1621 atggaccgta cggaatccgt caaggcccaa atgatcaaga aaggggttga ggttcctcct
     1681 gacgaggtat tgcaaaaaca gatccttgaa cgcctgattg ttgataacct gcaattgcaa
     1741 atggcggcac aaaccggcat caaggtggat gatgcccagc tggacaaaac catagagcgg
     1801 attgccgagc aaaacaacct caccttgccc gccttcaaaa aggcgctgga ggaagaaggc
     1861 accaggttct ataaattccg tgaagatatc cgcaatgaca ttatcctggc gcgcctgcgt
     1921 gagcgggacg tagacaacaa ggtcaacatt tccgaagccg agattgataa ttacctcagc
     1981 acacaagaga aagaaggcga cctcgatgaa ttcaatattt cacagatatt gattcgcttg
     2041 ccggaggaca gctccccgga ggatatccag aaagcccggg cacgcactga acaagtgatc
     2101 aaggcgctct ctgaaggcat gagttttgag aaagcctcag cgaatttttc agatgcgtca
     2161 aatgcattgg atggcggcac actgggctgg cgcagtggcc agcaaatgcc acctgaattc
     2221 cttgagctga tcaaaacatt gcaagtgggc ggtgtgtcac gtccgttccg tagtggcaat
     2281 ggggtacata tcctcaaatt gaatgagaga cgtgccggct ccagcaccct gattgttgaa
     2341 caaacccatg tgcgccacat cctgctaaaa cctaacgagg tactgtctga caaagaagct
     2401 aagcagaaaa tcgaaggtat caaggaacgc atagaccatg gcacgccatt ccaggatatg
     2461 gcacgtcaat actctgatga tggtagtgcg tccaatggcg gtgatctggg ctggatcagc
     2521 ccaggggaca cggtccctgt attcgaaaaa accatggcag aactggcaat taatgaggtc
     2581 agcgctccca tccgcagcca atttggctgg cacctgattc aagtgctgga gcgccgcaaa
     2641 caagatatgt ccaaagaatc caagcgcctg aaagcacgcc aggaaatccg ggcgcgtaaa
     2701 gctgaagagg cttataacga gtggatacac gagctgcgtg acaaagcctt tgtcgagctg
     2761 cgcttagaag acaaattcta acgaatgaac cacttaccac gtattgccat tacctctggc
     2821 gaacccgctg gcattggatt ggacatttgc gtcaagctcg ctcatacgcc tgttccagcg
     2881 gcgctggtgg tgttggctga tccaaacgct ttgcgagcca gggcatctca gttgggattg
     2941 ccgttaacga tcacgcctta caccgaggcc tccatagaac atgttggtga tggctgcttg
     3001 tctgtcttgg cgattgaaac agccgcgcct gtcatcgcag gtgagctcaa taaaaataac
     3061 agcaccgccg tattgaccat gctggatcgc gcactctctg gttgcctggc agatgaattt
     3121 gatgcgatgg tcacggcccc cgtgcataaa ggcattatca acgatgccgg catcacattt
     3181 accggacata ccgagtatct ggcagaaaaa accaatacgc cgcaagtggt gatgatgctg
     3241 gttgggggcg gtatgcgtgt cgcgctcgcc actacccatt taccattaac ccaagtcagc
     3301 gcagccatta cagcgagtgc cctgacgagc acaatccgta tcctgcatga cgacctggta
     3361 cacaaatttg ggatcgccca gcccagtatc tatgtggcag gtttgaatcc acatgccgga
     3421 gagaatggct atctggggac tgaagaaatt gaaaccatta acccggtact tgatcacttg
     3481 cgtgcagaag gcatgcaact gacgggtgcc ctgcctgcag acaccatgtt ttcgaaaaaa
     3541 aacctggcgc atgcagatgc ctttctggcc atgtaccatg accagggctt gagtgtactt
     3601 aagcacgcca gttttggtga gggcgtcaat gtcaccctgg gcttaccaat tattcgtact
     3661 tctgtcgatc atggcacagc gctggatatt gccggtagcg gccaagcaga tgttggcagc
     3721 atgctcagcg ccatccagct ggcgatcgac ttgtcagcat cacgccatac aaaacacgca
     3781 tgaaacatat cgccaagaaa aaatttggtc agaacttcct gatcgaccag ggcattattc
     3841 aaagcctgat caacgccatc cgtccacaag aggatgacct catggtcgaa atcggccctg
     3901 gcttgggcgc gatgacgcaa cccctgatca agcacctgaa tcacttgcat gtcgtggaaa
     3961 tcgaccgcga cattattcaa tggatgcaag gcttctaccc gcccaaaaaa atcaccatcc
     4021 ataattcaga cgtattaaag tttgatttcg ccaccatagg cgagcgcatc cgcgtggttg
     4081 gcaacctgcc atataacatc tccagcccta tcctgttcaa gctgctggaa aacacagcgc
     4141 agatcattga tatgcacttt atgctacaaa aagaagtggt cgaacgcatg gtggccgcac
     4201 cgtccagcgc ggaatatggc cgtttatcgg tcatgctgca gtaccgactg cagatggaat
     4261 acctggtgac tgtcccgcca gaagcctttg acccggctcc caaggttgaa tcagcgtttg
     4321 tacgctgtgt cccctatgcc acactgcctt atcctgccaa agatgaagcc ctgtttgcca
     4381 aaattgtcac cgcagctttt ggtcagcggc gtaagacctt gcgtaatacg ctaaaaggac
     4441 tgcttgatga cgatggattt accgccttga atctcgactc ccaactacgt gccgaaaatc
     4501 tgggcgtggc agaatttgtc gccattagta atcacctggc ggataacgct taaacctctc
     4561 aagcaaagct atgcctgcct ttcctgattt aaagcttagc ctgatataat ccttttttta
     4621 ctaaaaccca aacttttgga gcatgttatg cgtttgattc tgctgggcgc ccctggtgcc
     4681 ggtaaaggga cacaagccac ctttattaaa gaaaaattca atattccgca aatttccact
     4741 ggcgatatgt tgcgcgcagc agtcaaggca ggcacccaat taggtctgga agccaaaaaa
     4801 ttcatggatg cgggcggcct ggtacccgat gaagtcatta tcggcctggt gaaagaacgc
     4861 attaaagatg ca
//
LOCUS       DATMSK010000196        11538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_226055,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000196 DATMSK010000000
VERSION     DATMSK010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11538
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226055"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..2723
                     /gene="leuS"
                     /locus_tag="VNZ84_06425"
     CDS             108..2723
                     /gene="leuS"
                     /locus_tag="VNZ84_06425"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWU66629.1"
                     /translation="MQQQYPFKEVEQQAQQHWDSNLSFQAKADSSKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFHHMKGFNVLQPMGWDAFGLPAENAAIQNSVPPAQWTY
                     DNIAYMRKQLKSLGFAIDWSRELATCQPDYYKWNQWLFLRMLEKGIAYKKTQVVNWDP
                     VDQTVLANEQVIDGRGWRTGAVVEKREIPGYYLNITGYAQQLLDDLDKLPGWPERVKT
                     MQTNWIGKSVGVRFAFTHEIKNSEGQLIDDGKLWVFTTRADTIMGVTFCAVAAEHPLA
                     THAAQHNPALQAFIEKCKQGSVMEADMATMEKEGMDTGLTVAHPITGEQVPVWVGNYV
                     LMTYGEGAVMAVPAHDERDFGFAKKYQLPIKQVISVVGQEFDLDGWQEWYGDKSKGVC
                     IHSGKYNDLGYTEAVDAVATDLAEKNLGGKQTNWRLRDWGVSRQRYWGCPIPIIHCDS
                     CGDVPVPDDQLPVKLPEDCVPDGSGNPLNKREDFLNCACPKCGQPAKRETDTMDTFVD
                     SSWYYARYASGFSDTAMVDAQTNHWMPVDQYIGGIEHAILHLLYSRFWSKVMRDLGLV
                     NYDEPFANLLTQGMVLNHIFSRRTAKGGIEYFAPEEVELVHDDNGKISGAKLVSDGST
                     IDYQGIGTMSKSKRNGVDPQSLIEQYGADTARFFMMFAAPPEQTLEWSDSGVEGSHRF
                     LKRVWNFGYALSEVQPVDMPTKLTGELANHRREVHSVLKQANVDLAKLQFNTVASACM
                     KILNTLEKVQKEAEKDWQAVAFEGYSILLRVLSPIAPHITQVMWQSLKLGTDILTAKW
                     PEPANSALVQDEVEYVVQVNGKLRGSISIPKSMAKEQIEATAVNQDFVQKFLNEGSVK
                     KIIVVPNKLINIVVA"
     gene            2739..3248
                     /gene="lptE"
                     /locus_tag="VNZ84_06430"
     CDS             2739..3248
                     /gene="lptE"
                     /locus_tag="VNZ84_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWU66630.1"
                     /translation="MLQILKHKSLTYWLSMLTLSVMLTACGFHLRQPSPIAFKTIHIK
                     GTTLINTALKKALTEQGIKVVADPEDAELQLDLLREENEQRILSLSGTGVVREYELYY
                     RVQYRTKMADEATWTLPLIMEGRRDYTYNDANLLAKQAEQKLLTESMQTDVLNGILRR
                     LSALKKAPQ"
     gene            3251..4294
                     /gene="holA"
                     /locus_tag="VNZ84_06435"
     CDS             3251..4294
                     /gene="holA"
                     /locus_tag="VNZ84_06435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWU66631.1"
                     /translation="MRIQVAQLTQHLPPKQHLFVLAGDEPLSITEAIDQIRTAARQHG
                     AQERASFTVERYFNWGQVSQFLQSFSLFAEQRILEINIPTGKPGVDGAKALQQLAELP
                     AADTTIIITLPAPDRDMTSSAWYEALSQHGVLLVLNQVPLAQLPEWIAQRLALQQQTA
                     DETSRRFIAEQVEGNLLAAHQEIQKLGLLYPPGPLTEVAIRQCVLNVARFDVSQLGEA
                     MLQGDQSRVMRIIEGLREEGETAVAVMNPLVWLFRPLLQVRLAMQNGLAAQAAMSQAR
                     LFGDRQILVKRALEFLPQKHIEAALQKLSDIDRMAKGVGDGDAWLELSRLCSGIARMA
                     ANKSARAAAARSR"
     gene            4318..5574
                     /locus_tag="VNZ84_06440"
     CDS             4318..5574
                     /locus_tag="VNZ84_06440"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWU66632.1"
                     /translation="MDIQHYMKQLGEQARAASRLMAKADTNTKNQALRNIAALIRQHE
                     KALLAANQQDMDAAKANGMEAAMLDRLALSEKSVATMAEGLEQIASLPDPIGEMSNFK
                     YRPSGIQIGQMRVPLGVIGIIYEARPNVTVDAAGLCIKSGNACILRGGSEAIHCNQAL
                     AKLVHQGLQQAGLPQAAVLVVETTDRAAVGELITMKQYVDVIVPRGGKGLIERISNDA
                     RIPVIKHLDGNCHVYVDDEADINKAVRILENAKTQRLGTCNTAESLLVARSIANTALP
                     KLAEMLLSKSVEIRGCDETRAIVPQAKAATEEDYYTEYLDSIISCKVVSGLDEAIEHI
                     NKYSSQHTEAIVTENYTKARQFLREVDSSSVMVNASTRFADGFEYGFGAEIGISTDKL
                     HARGPVGLDGLTSLKYVVLGDGHVRA"
     gene            5732..6373
                     /gene="nadD"
                     /locus_tag="VNZ84_06445"
     CDS             5732..6373
                     /gene="nadD"
                     /locus_tag="VNZ84_06445"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWU66633.1"
                     /translation="MELIGIFGGTFNPIHNGHLALAQTVLDTLHCTQIRVIPAAVPPH
                     KEAPAVTAQHRATMVKLAIQSQPTFVLDTCELDRQGPSYTIETLHLLRTRFPDQALCL
                     MMGQDSYQKLPTWHRWLELLDYCHLLVVHRADKEKTLTLHPEHEGKLVDIATAPQAFS
                     AHANGLISFLTQAPPDISSTRTREQLRQADNAVRNQIPSAVWQYIQENRLYQT"
     gene            complement(6437..8635)
                     /locus_tag="VNZ84_06450"
     CDS             complement(6437..8635)
                     /locus_tag="VNZ84_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC domain/YcaO domain-containing protein"
                     /protein_id="HWU66634.1"
                     /translation="MEVKVNFLDKLRLEAKFDDFTVIADQPIRYKGDGSAPGPFDYFL
                     ASSALCAAYFVKLYCSTRGIPTENIRLSHNNIVDPENRYKQIFKIQIELPADISEKDR
                     IGILRSIDRCTVKKVVQEGPDFIIEEVANLDADAQALLTVDTSAASQTYITGKDLPLE
                     QTIANMTGLLANLGIKIEIASWRNIVPNVWSLHIRDAHSPMCFTNGKGATKESALASA
                     LGEYIERLSNNHFYAGSYWGEEIANAPFVHYPNERWFKPGRKDALPKEILDEYTLEIY
                     NPDGELRGSHLIDTNSGNVERGICSLPFVRQSDGETVYFPTNLVENLFVSNGMSAGNT
                     LVEAQVQCLSEIFERAVKREIIEGEIALPDVPQDVLAKYPGVVAGIKGLEEQGFPVLV
                     KDASLGGQYPVMCVTLMNPRTGGVFASFGAHPTMEVALERSLTELLQGRSFEGLNDLP
                     QPTFASEAVTEPNNFVEHFIDSSGIVSWRFFSAKSNYEFVEWDFSGEGEDSNAQEAAT
                     MFGMLADMGKEAYVAVYDQLGAVACRILVPGYSEVYPVEDLVWDNTNKALLFREDILN
                     LHTLDDASLEALLDRLENNELDDYGDIATLIGIEFDENTDWGQLTVMELRLLIHLALQ
                     QFEEAHDLVGAFLQYNDNSLERGLFYQALNVVLEVELDNELDMADYEASFRKMFGDTR
                     MDAALGSVDGSVRFYGLTPTSMKLEGLDRHHRLIDSYKKLHAARVKWTAK"
     gene            8922..11513
                     /locus_tag="VNZ84_06455"
     CDS             8922..11513
                     /locus_tag="VNZ84_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU66635.1"
                     /translation="MAADDRKFITLTDFLHEPVIHYDRDCKRTYLNPAAIKLIGEPSK
                     DLEGKTPSSLRPSSQALQHYQEKIRGVIETGVDVEFDFFLDRLPDGPNVHAKVLRIRA
                     VPELDSEGKVVGVLAIAHDVTALKLNKKRLTALIENHPDHITRFDLQARYSYVSPSFT
                     ALLSIEESTLLGKTLPECEQIATAAERERIHQAIWQTLQQQTSCTLESYWPNDRVIEL
                     RLVQEKDAIGEISGVLCILRDITQQKHSEKALLTLNRSLRLLSSCNQTLIHAENEQQL
                     LNDICQLVIESGGYRLSWVGYPERGTYQPLRIAALAGEGLTNPETIALEQQTPEISLA
                     VKAFHSGRVEIIQDYPDAAALNSLGEMASREGLLSCIALPLKVAKKTIGVITIYASQT
                     HAFSDEEVTLLDELANDLAFGIQSLRIRAEHRDAETKLEYLAHHDPLTGLPNRLLLRK
                     SFDQCIHQANKRDYGLAMLFLDMDNFKEINDSFGHATGDALLVEVVRRLERELSESCT
                     LSREGGDEFLLLINNTHGSEIVTQTAQQILNAMQEPFEVSGNTLHASFSIGISLYPND
                     GKDFETLRKNADAALYLAKDCGRNTYRFFVAQMNADAQKRMRIQTDMHTALKNNEFLL
                     HYQPQVRVCDGRISGIEALIRWQNAEGQMLSPCEFIPIAEQSGMIIQMGQWVLREACR
                     QLAVWHQQGHTQLVVAVNLSSHQFRHGNIVETISAALHDFGLPPQCLELELTESILIQ
                     DTESVISTLHTLKHIGVKLAIDDFGTGYSSLSYLKQLSVDKLKIDKSFIRDLNQDKDS
                     AEIVRAIIQLGHILQLTVVAEGVETEEQLVYLANSYCDEMQGYLFSRPVAAEHIPEIL
                     DRVGVRT"
BASE COUNT         2949 a         3253 c         2873 g         2463 t
ORIGIN      
        1 accaccaatc atgcgaatcc cttcctgagt gaccgctaag tcaaaacatg cccatgcgtt
       61 ctcgggtata attgaagctt ccttttagtt acgttcaagc caaccacatg caacagcagt
      121 atccattcaa agaagtcgaa caacaggcac aacaacattg ggactccaac ctcagctttc
      181 aggccaaagc tgatagcagc aaacccaaat actactgttt gtccatgttc ccctacccta
      241 gtggcaaact gcatatgggg catgtacgca actacaccat tggagacgtg ttaagccgtt
      301 tccaccatat gaaaggcttt aacgtactgc agccgatggg ttgggatgcg tttggcctac
      361 cggctgaaaa tgcagcgatc cagaacagtg tgccacccgc acaatggacg tatgacaata
      421 ttgcctatat gcgcaaacag ctcaaatcgc tgggctttgc catcgactgg agtcgtgaac
      481 tggctacctg ccagccagac tactataaat ggaaccagtg gcttttcctg cgcatgctgg
      541 aaaaaggcat tgcctacaaa aagacccagg tcgtgaactg ggacccggtc gaccagactg
      601 tgctcgccaa tgaacaggtg attgatggcc gcggctggcg tacaggcgcc gtcgtggaaa
      661 aacgcgaaat ccccggttac tacctgaata tcaccggcta cgcccagcaa ctgctggatg
      721 acctggacaa actgccaggc tggccagaac gagtcaaaac catgcagacc aactggatcg
      781 gtaagagtgt aggcgtgcgt tttgccttta cacacgagat caaaaacagt gaaggccagc
      841 tgattgatga cggcaaactg tgggtattca ctacccgtgc cgataccatt atgggcgtga
      901 ccttctgtgc tgtggcggcc gagcatccac tggccaccca tgctgcgcaa cacaatccag
      961 cactgcaagc ctttattgaa aaatgcaaac aaggctccgt catggaagcc gatatggcaa
     1021 ccatggaaaa agaaggcatg gacaccggcc tgaccgtcgc ccacccgatt accggcgaac
     1081 aagtgccggt ttgggtaggt aactatgtac tcatgaccta cggtgaaggc gcggtgatgg
     1141 ctgtacccgc acatgatgaa cgtgattttg gctttgccaa aaaatatcag ctccccatca
     1201 aacaagtgat tagcgtcgtc ggccaggagt ttgacctcga tggctggcag gaatggtatg
     1261 gtgacaagag caaaggcgtc tgtatccact ctggcaaata caacgatctc ggctacaccg
     1321 aggctgtaga tgctgtggcc accgacttgg ccgagaagaa cctgggtggc aagcaaacca
     1381 actggcgcct gcgtgactgg ggcgtctccc gccagcgcta ctggggctgc ccaatcccga
     1441 ttatccactg cgacagctgt ggcgatgtgc ctgtgccaga tgatcaattg ccggtcaaac
     1501 tgccggaaga ttgcgtgccg gatggttccg gcaatccact caataaacgc gaagacttcc
     1561 tgaactgcgc ttgccccaaa tgcggccagc cagccaaacg cgaaaccgat accatggata
     1621 cctttgtcga ctccagctgg tactacgcac gctatgcctc cggcttcagc gacacagcca
     1681 tggtggatgc tcaaaccaac cactggatgc cggtcgacca atatatcggc ggcattgaac
     1741 atgccattct gcacctgctg tactcccgct tctggagcaa ggtcatgcgt gaccttggtc
     1801 tcgtcaatta cgacgaaccc tttgccaacc tgctgacgca gggtatggtg cttaaccata
     1861 tcttctcgcg ccggactgcc aaaggcggca ttgaatattt tgcgccggaa gaagtcgaac
     1921 tggtccatga tgacaacggc aaaattagcg gtgcaaaact agtgagcgat ggctctacca
     1981 ttgactatca gggcataggt accatgtcca agtccaagcg taatggtgta gacccacaat
     2041 ctctgattga gcaatatggt gcagacaccg cgcgtttctt catgatgttt gcggcgcctc
     2101 cggagcaaac cctggagtgg tcagacagtg gcgtggaagg ctcccaccgc ttcctcaagc
     2161 gtgtatggaa ctttggctat gccttgtcag aggtccagcc agtggacatg ccaaccaaac
     2221 tgacgggcga gctggccaac cataggcgcg aagtccacag cgtactcaaa caggcgaatg
     2281 ttgacctggc caagctgcag ttcaacacag tcgcttctgc ctgcatgaaa atcctcaaca
     2341 cactggaaaa agtgcagaaa gaagccgaga aagactggca agccgtggct tttgaaggct
     2401 acagcatcct gttgcgcgtg ctgtcaccga ttgcaccaca cattacacaa gtcatgtggc
     2461 aaagcctgaa gctggggacg gatatcctga ccgccaaatg gccagaaccg gccaactctg
     2521 ctttggtgca ggatgaagtc gaatacgtgg tgcaggtcaa cggcaagctg cgcggcagca
     2581 tcagcattcc caaaagcatg gccaaggaac agattgaagc cactgccgtc aaccaggatt
     2641 tcgtccagaa attcctcaat gaaggcagcg tcaaaaaaat tattgtggtg cctaacaaac
     2701 tgattaatat cgtggtggct taacccaagg agcaaacgtt gctgcagata ctcaagcata
     2761 aaagtctgac ttactggttg agcatgctga ctctgtcagt catgctgact gcctgcggct
     2821 ttcacttgcg ccagccttcg ccgattgcct ttaaaaccat ccatatcaaa ggcacgaccc
     2881 tgattaatac agctctcaaa aaagcgttaa cggaacaagg catcaaagtc gttgccgatc
     2941 ctgaagatgc agagctgcaa ctggatctgc tcagggaaga aaacgagcaa cgcatcctgt
     3001 ccttgagcgg taccggcgtg gtccgggaat acgagcttta ctatcgcgtg caataccgca
     3061 ccaaaatggc agacgaagcc acatggacat taccactgat tatggaaggc cgtcgcgact
     3121 acacctataa cgacgcaaac ttgctggcca aacaggcgga acaaaaactg ctgacagaaa
     3181 gcatgcagac cgatgtgctg aacggcatcc tgcgccgcct gtctgcattg aaaaaagcgc
     3241 cacaataacc atgcgcatcc aggttgcgca actgacccaa cacctgccgc caaaacaaca
     3301 cttgttcgtg ctggcgggtg atgagccgct ctcaatcaca gaggccatag accagatccg
     3361 cacagccgcc cgccagcatg gtgcgcaaga acgcgccagc tttacggtgg agcgctattt
     3421 caactgggga caagtcagcc agttcctgca aagtttctcg ctgtttgccg agcaacgtat
     3481 ccttgagatt aacattccca ccggcaaacc cggcgtggat ggtgccaagg ccttgcaaca
     3541 actggccgaa ctgcctgcgg ccgataccac cattatcatc accctgcccg cgccagaccg
     3601 ggacatgacc agcagtgcct ggtatgaagc gctgtcgcag catggcgtac tgctggtgct
     3661 taaccaggtg ccactggcac aattgccgga gtggattgcg caacgcctgg ccttacagca
     3721 acaaacggcg gatgagactt cacgccgctt tattgccgaa caggtagaag gcaatttgct
     3781 tgccgcgcac caggaaatcc agaagctggg cttactctac ccgccagggc ctctgacaga
     3841 agtcgctatc cgccaatgcg tgctcaatgt ggcgcgcttt gatgtctcgc agctggggga
     3901 agccatgctg caaggcgacc agagccgcgt catgcgcatt attgaaggcc tgcgtgaaga
     3961 aggtgaaacc gccgtcgccg tcatgaaccc gctggtctgg ttattccgcc cgctcttgca
     4021 ggtacgcctc gccatgcaaa acggcctggc cgcacaagct gccatgagcc aggcccggtt
     4081 atttggggat cgacagatac tggtgaaacg cgcattggaa tttttaccgc aaaaacatat
     4141 cgaagctgct ttgcaaaagt tgtcagacat agaccgtatg gcaaaaggcg tgggcgatgg
     4201 cgatgcctgg ctggaactct ccaggctatg tagcggcatc gcgcgtatgg cggcgaataa
     4261 atccgcccgg gcagctgccg caaggtctcg ttaaggtttt aagatagaat gatggttatg
     4321 gatattcaac actacatgaa acaactgggt gagcaggcgc gtgccgcttc ccgtttgatg
     4381 gcaaaagccg ataccaacac caagaatcag gccttgcgta atattgccgc cttgatccgc
     4441 cagcatgaaa aagcgctgct tgcggccaac cagcaagaca tggacgctgc caaagccaac
     4501 ggcatggaag ccgccatgct ggaccgcctg gccctcagcg aaaaatctgt cgccaccatg
     4561 gcggaaggcc tggaacagat tgccagcctg ccagacccga ttggcgaaat gagcaacttc
     4621 aaataccgcc catccggcat ccagatcggc cagatgcgtg tgcctctggg tgtgatcggc
     4681 attatttatg aagcacgtcc taacgtgacc gtcgatgcgg ctggcctgtg catcaaatcc
     4741 ggcaatgcct gtattctacg tgggggctct gaggccatcc attgcaacca ggcactggcc
     4801 aaacttgtgc accagggcct gcaacaggca ggcttgccgc aagctgccgt gcttgtggtg
     4861 gaaacgacag accgcgccgc cgtgggtgag ctcatcacca tgaagcaata cgtcgatgta
     4921 attgtgccac gcggtggcaa aggcctgatc gagcgtatca gtaacgatgc gcgtatcccg
     4981 gtcatcaaac accttgacgg caattgccat gtctatgtcg atgacgaagc ggatatcaac
     5041 aaagccgtac gcatcctcga aaacgccaaa acccagcgcc tgggcacttg caacacggca
     5101 gagtcattgc tggtagcccg cagcatcgcc aacaccgccc tgcccaaact ggccgagatg
     5161 ctgctcagca aaagcgtgga aatccgcggc tgcgacgaaa cccgcgccat cgtgccacaa
     5221 gccaaggcgg cgaccgagga agactactac accgagtacc tggattcgat tatctcgtgc
     5281 aaggtagtga gtggcctgga tgaagccata gagcacatca ataaatactc cagccagcac
     5341 accgaagcga ttgtgaccga aaactacacc aaggcacgcc agttcctgcg cgaagtggat
     5401 tccagcagtg tcatggtcaa cgcctccacg cgctttgccg atggctttga atacggcttt
     5461 ggtgcagaaa tcggtatttc taccgacaaa ctgcatgcac gtgggccagt cggattagac
     5521 gggctgacgt cactgaaata tgtcgttcta ggcgacggcc acgttcgagc ataaattgca
     5581 tgaattgggc acatcacgtt gttacgcggt gctcgaaatc cccatgtact ctggtttttg
     5641 tgctccgtgc gcgccagcgc tcaatacaaa gcactgacgc acaattgatg caatttgatg
     5701 attgctgcga tgcgccgaaa gaagcattaa gatggaactc ataggcattt ttggcgggac
     5761 gttcaatccc atccacaacg ggcacctagc cctggcgcaa accgtgcttg ataccctgca
     5821 ttgcacacaa atccgcgtga ttcctgccgc cgtgccaccc cacaaagaag cgcctgccgt
     5881 caccgcccag caccgtgcga ccatggtcaa gctggcgata caaagtcagc caacatttgt
     5941 actggatacc tgcgaactgg accgccaggg tccttcctat accatagaaa ccctgcatct
     6001 gttgcgtacc cgttttcctg accaggcctt atgcctgatg atgggccagg acagttacca
     6061 aaaactgccg acctggcacc gctggctgga gctgctggac tattgccacc tgctggtggt
     6121 acaccgggcc gataaggaaa aaaccttaac cttgcatcca gagcacgaag gtaaactggt
     6181 agatatcgcc acagccccac aagcattttc tgcgcacgca aatggtttaa tcagcttttt
     6241 aacccaggca ccgccagata tttcctccac ccgtacccgt gagcaacttc gtcaggctga
     6301 caatgccgtg cgcaaccaaa tcccctcagc ggtctggcaa tatatccagg aaaatcgcct
     6361 gtaccaaaca taaaagccac agtgttccaa cacggtggct ttgtttaatt caacctaggt
     6421 gactggacta agtcgcttat tttgcagtcc atttgaccct ggccgcatgg agcttcttat
     6481 agctgtctat caggcggtga tgcctatcta gcccttccag tttcatactg gttggcgtca
     6541 aaccgtagaa gcgtacgctg ccatctaccg agcctaatgc ggcgtccatg cgcgtatcac
     6601 caaacatttt gcggaagctg gcttcgtaat ccgccatgtc caactcgtta tccagttcta
     6661 cttccagtac cacattcaaa gcctggtaaa acaggccgcg ttccagcgag ttatcgttat
     6721 attgcaaaaa ggcacccact aggtcatgcg cttcttcaaa ctgctgcaag gcgagatgaa
     6781 tcagcagtct taattccatc accgtaagct ggccccaatc ggtattttcg tcaaactcaa
     6841 tgccgatcaa ggtggcaata tcgccgtaat catccagctc gttattttcc agacggtcca
     6901 gcaacgcttc caggctggca tcgtccagtg tgtgcaggtt gaggatatct tcacggaaca
     6961 atagcgcttt attggtgtta tcccacacta gatcttctac cggataaact tcagaatatc
     7021 ccggcaccag gatacggcag gcaactgcgc ctaattggtc atacacggcg acatatgcct
     7081 ctttgcccat gtcggccagc atgccaaaca tggtcgcggc ttcctgagca tttgaatcct
     7141 caccctcacc ggaaaaatcc cattccacaa attcgtaatt cgacttcgcg ctgaaaaacc
     7201 gccacgacac aatgccgctg gaatcaataa agtgttcgac aaagttattc ggttcggtca
     7261 ccgcttcgct ggcaaaagtc ggttgcggca aatcattgag gccttcaaag ctgcggccct
     7321 gcagtaattc ggtcaagctg cgttccagcg cgacttccat ggtcgggtgc gcgccaaacg
     7381 aggcaaacac accgcccgtg cgtgggttca tgagggtcac acacattaca ggatattgcc
     7441 cgcccagcga ggcatctttc accagcaccg ggaatccctg ctcctccagc cccttgatac
     7501 ccgccaccac gccagggtat ttcgccagca cgtcctgcgg cacatccggc aaggcgattt
     7561 cgccctcaat gatttcgcgt ttgactgcac gctcaaaaat ttctgacaag cattgcacct
     7621 gcgcttccac cagcgtattg cctgcactca tgccattgct gacaaacagg ttttccacca
     7681 ggttggtcgg gaagtacacg gtttcaccat ccgactgacg cacaaacggc agcgaacaga
     7741 taccgcgttc cacattgccg gaattagtgt ctatcaggtg cgaaccacgc agctcgccat
     7801 ccgggttgta aatctccaag gtgtattcat caagaatctc tttcggcagc gcatccttgc
     7861 ggccaggctt gaaccagcgc tcgttcgggt aatgcacaaa tggcgcattg gcaatctctt
     7921 cgccccagta cgatccggca taaaaatggt tgttactcag ccgctcgata tattcaccca
     7981 gcgctgaagc cagcgcgctt tccttggttg cacctttgcc attcgtgaag cacataggtg
     8041 aatgcgcatc gcggatatgc agcgaccaca cattaggcac aatattgcgc cacgacgcga
     8101 tctcgatctt gatgcccaga ttcgccagca gcccggtcat attggcgata gtttgttcca
     8161 gcggtaaatc tttgccggtg atgtaggtct gtgacgctgc cgatgtatcc accgtcagca
     8221 aggcctgtgc gtcggcgtct aaattagcca cttcttcaat aatgaaatcc ggcccttcct
     8281 gtaccacttt cttcactgtg cagcggtcaa tcgaacgcaa aatgccaatg cggtcttttt
     8341 cagaaatatc ggcaggcaac tcgatttgta ttttgaaaat ctgtttgtag cggttttccg
     8401 ggtccacaat attgttgtgc gacaaacgga tattctcagt cggaatcccg cgcgtgctgc
     8461 aatacagctt cacaaaatac gctgcacaca aggctgatga cgccaggaaa taatcgaacg
     8521 ggcccggtgc cgagccatcg cctttatagc ggataggctg gtcagcaatc accgtgaaat
     8581 catcaaactt ggcctcaagg cgcagcttat cgagaaaatt gactttgact tccatggaag
     8641 aattccaaca atgacattta aaacaaagac cgacattatc gcccacaaac tacaggcaac
     8701 ctaatggtgt tgccgggttt gttgctgaaa gtagaggact aatcggtgcc tgtgattttg
     8761 cgggccatga tttttcaacg ccctgcgcca atgaagcggg tgcatcatgt ctctattctg
     8821 acaattggct agcaaccaag catcgtaaaa tgctctgaaa acgataaagt ttgtgtgata
     8881 ttctttaacg agtaaaacta tattcttcgg ggatgacaac catggctgct gatgacagaa
     8941 aattcatcac gctgacagat tttctgcatg aaccagtcat acattatgat agggattgca
     9001 agcgcaccta tctcaacccg gctgcaatca aactgattgg tgaaccctct aaagacctgg
     9061 aaggcaaaac acccagctcg ttgagaccct cttcacaagc cttgcaacac tatcaggaaa
     9121 agatccgtgg cgtgatagaa accggggtag atgtcgaatt tgatttcttt ctcgaccgac
     9181 tgcctgatgg cccgaatgtc cacgccaagg tactcagaat tcgtgcagtt cccgagctgg
     9241 atagtgaggg caaagtggtg ggcgtcctgg cgattgccca tgatgtcacc gcactcaaat
     9301 taaacaaaaa gcgcctcacc gccctcattg aaaaccatcc cgaccatatc acccgttttg
     9361 acctgcaggc cagatacagc tacgtcagcc cttcatttac agccttattg agcatagagg
     9421 aatccacgct gctgggcaaa accttacctg aatgtgaaca gattgcaact gccgcagaac
     9481 gcgaaagaat tcaccaggca atctggcaaa cattgcagca acaaacgtct tgcaccctgg
     9541 agagctactg gccgaatgac cgcgtcatag aacttcgcct ggtccaggaa aaagatgcca
     9601 tcggcgagat ttccggcgtg ctctgcatct tgcgggatat cacccagcaa aagcattccg
     9661 aaaaagcgtt gctgacactc aatcgctcat tacgtttact cagcagctgt aaccagacac
     9721 ttattcatgc cgaaaatgaa caacaactgc tcaacgacat ttgccagttg gtgatcgaat
     9781 ccggtggcta ccggctgtca tgggtcggtt atccagaacg tggtacctac caaccgttgc
     9841 gtattgcggc cttggccggt gaagggctga ccaatccgga gacgattgcg cttgagcagc
     9901 aaacgccaga aatcagcctg gccgtcaaag cctttcactc cggccgtgtg gaaatcatcc
     9961 aggactaccc cgatgcagcg gcgttgaaca gcctcggcga aatggccagc cgcgaaggcc
    10021 tgctttcctg tatcgccctg ccgctcaaag tagccaaaaa aaccataggc gtcatcacga
    10081 tttatgcctc ccagacccat gcgttttcgg acgaagaagt cacgctgctc gatgaactcg
    10141 ccaatgacct ggcgtttggc atccagtccc tgcggatccg ggccgaacac cgggacgcag
    10201 aaacaaaact cgaatatctt gcccaccatg acccgctgac cggcctgcct aaccgactgc
    10261 tactgcgcaa aagttttgat cagtgcatac accaggccaa caagcgcgac tacggcctcg
    10321 ccatgctgtt tcttgacatg gacaacttca aggagatcaa cgacagcttt ggtcatgcca
    10381 ccggagatgc gctgctggta gaggtggtca ggcgcctgga acgcgagctt tcagagtctt
    10441 gcaccctgag ccgtgaaggg ggtgacgagt ttttgctgct catcaataac acccatggct
    10501 cggagatcgt cacccaaacc gcgcagcaga ttctcaacgc aatgcaagag ccgtttgagg
    10561 tcagcggtaa cacattgcac gcctctttca gcatcggcat cagcctctat ccaaacgatg
    10621 gcaaagactt tgaaacccta cgcaaaaacg ccgatgccgc cttgtacctg gcaaaagatt
    10681 gtggccgtaa tacctacagg ttttttgttg cgcagatgaa tgccgacgcg cagaaacgca
    10741 tgcgcatcca gactgacatg cacacggcac ttaaaaacaa tgagtttttg ctgcactacc
    10801 aaccccaagt acgcgtttgt gatggccgca tcagcggcat tgaagcccta atccgttggc
    10861 aaaacgcaga ggggcaaatg ctctccccgt gcgaatttat tcccatcgca gaacaaagtg
    10921 gcatgattat ccagatgggg cagtgggtgc tgcgggaagc ctgccgacaa ctggccgtct
    10981 ggcatcagca agggcacaca cagctggtgg tggcagtcaa cttatcctca caccagttcc
    11041 gccatggcaa tattgtagaa accatcagcg cagccctgca tgattttggc ctgcccccgc
    11101 aatgcctgga gctagagctg acagaatcta tcctgatcca ggacacagag tccgtgatca
    11161 gcacgctgca cacgctcaag cacatcggcg tcaaactggc cattgatgac tttggcaccg
    11221 gctattccag cctgtcatac ctcaaacaac tatcagtcga caaactcaag attgataaat
    11281 cctttatccg cgatctaaat caggacaagg acagtgccga aattgtccgc gcgatcatcc
    11341 agcttgggca tatcctgcaa ctgacggtgg tggccgaagg ggttgagact gaagagcaac
    11401 tggtctatct cgccaatagt tactgtgacg aaatgcaagg atacttgttt agccgcccgg
    11461 tggccgcaga acacattcca gagatacttg atcgagtagg cgtgaggacc tgatccagta
    11521 tttttttcgc aaaacctt
//
LOCUS       DATMSK010000197         4832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_132331,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000197 DATMSK010000000
VERSION     DATMSK010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132331"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..974)
                     /gene="cyoE"
                     /locus_tag="VNZ84_06460"
     CDS             complement(78..974)
                     /gene="cyoE"
                     /locus_tag="VNZ84_06460"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWU66636.1"
                     /translation="MQTTWMTQLATAKMGLRHFYALCKPRVTALIVFTAIIGMLMATP
                     GMVPASVLLAATVGIAFASGAAAAFNCLIEHKIDAMMARTRARPLPTGQLSQMETLLF
                     ASVLGGIGLSILYYWVNPLTMWLTLGTFVGYAVIYTVFLKPATPMNIVIGGASGAMPP
                     ILGWAAVNNTVSPESLVMFLIIFAWTPPHFWALALYRREEYAKVGMPMLPVTHGEQFT
                     LLHILLYTVILVVVSLMPYGLGMSGWLYLASAAVLNLIFMYYVVGLYRRYSDALAKTT
                     FKYSIIYLSLIFAALLLDHYLV"
     gene            complement(1000..1971)
                     /locus_tag="VNZ84_06465"
     CDS             complement(1000..1971)
                     /locus_tag="VNZ84_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HWU66637.1"
                     /translation="MFKALSITAAIVAFCVVVLGSYVRLSDAGLGCPDWPGCYGALTV
                     PQSEAAIESAQQAYPDKPVEHAKAWKEMAHRYLAGSLGLLVAGLAVLAYRQRLTIRTS
                     WQLSVALVVLIIMQALLGMWTVTLLLKPVIVTLHLLGGMTTLAVLSWIAFRHTCKADI
                     ALPADQQYWLRLALLIWIAQVLLGGWTSSNYAALACTDFPTCHSLWWPAMDMGDAFHI
                     TRALGVSRDGGQLHLDALTAIQWVHRLGAMVVLVWMGMSTRILLQRQTSKMLAWALLA
                     IVLCQIAVGIGNLLLQLPLVLAVLHNAGAALLGVCLVAINARLTKTS"
     gene            complement(1975..2595)
                     /locus_tag="VNZ84_06470"
     CDS             complement(1975..2595)
                     /locus_tag="VNZ84_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66638.1"
                     /translation="MTHEEANSLTAIDPTQQRKGRMIFLLLAIFFTVPLLVVVAMFKF
                     DWRPSGKSYGQLIQPPVSIAYASHWVSDQQQALPAFWQDKWNIVLIARECDDICMQRL
                     YDLRQIYVSLYKDMIRVQRVLITQQTDTAPIRARYPDLLIINGESEQVTDLGRMLSGG
                     GQNTLDARRVYFIDPLGNIMMQYGPEMEAKWIRKDLVKLLKASWAG"
     gene            complement(2598..3323)
                     /locus_tag="VNZ84_06475"
     CDS             complement(2598..3323)
                     /locus_tag="VNZ84_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HWU66639.1"
                     /translation="MQIPLGNYVIRVRWMVVCVMVAAMWGCVRLGLWQWHKAEQKQEI
                     TRALTYHAGAIQVQAFVLDDAAKVQALHLQTVELTGHYLPEFSFLLDNQVEQGQAGFH
                     VITPFLLKDQQHVVWVNRGWIAGFSDHQKTPVVATPQTEKIIKGLFWQQTKIGFRLDK
                     PGQAWEAVQPVIDFAYLRQHVPYTFPDVIIKLDSGMPDGYVRHWEVPSGQVEKHLSYA
                     YQWFGFALASLAIGVYQMVEKRA"
     gene            3373..3573
                     /locus_tag="VNZ84_06480"
     CDS             3373..3573
                     /locus_tag="VNZ84_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HWU66640.1"
                     /translation="MFIKIATILILITIIGSLFSALLFLLKDKRGSERTVKALTMRIG
                     LSIFLFLALVIAAHFGYVGHTL"
     gene            complement(3620..4552)
                     /locus_tag="VNZ84_06485"
     CDS             complement(3620..4552)
                     /locus_tag="VNZ84_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWU66641.1"
                     /translation="MASHSENHYFVPHGSIYPALISVGLLSMASGFVFNVTGSDTRMD
                     GSIKNPALSYLATPGKYMMYIGLAIVLTMMFKWLSTVVTESITGQYKKWEDKSFRIGM
                     IVFICSEVAFFAAFFGALFYMRVLSVPDLASYAPDITPYKDFLSTWPSQGPGGTVLGE
                     TYKPNTGFHPMTWSGLPLINTLLLLSSGATITWAHWGLIKNNRKQLIVGLFLTIALGV
                     TFLCCQAAEYHHAYTEMGLTLKSGAYGATFFMLTGFHGFHVTIGTLMLIVIWLRSIKG
                     HFTPEHHFGFEGVAWYWHFVDVVWLGLYIFVYML"
     gene            complement(4596..>4832)
                     /locus_tag="VNZ84_06490"
     CDS             complement(4596..>4832)
                     /locus_tag="VNZ84_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWU66642.1"
                     /translation="MFEAKNITNEEVAGQAIPSVTPGIASAHLKKIECFCFVRQTLKP
                     GEVKALPLRFFISADLPQDVQEMTLSYSFFPASQ"
BASE COUNT         1368 a         1324 c         1028 g         1112 t
ORIGIN      
        1 accgcattca tcggctgatg aacagcgaga ccaaacatta acagtcagct ggtagcgcaa
       61 atgcgctacc agtgagctca caccaaatag tgatcgagca ataacgcagc aaatataaga
      121 ctcaagtaaa taatggagta cttaaaggtt gttttggcga gtgcatccga atagcggcga
      181 tacaagccga ccacgtaata cataaaaatg agattgagta cggccgccga agccaggtag
      241 agccagccac tcatccccag gccgtaaggc atcagggata caacaaccag aatcaccgtg
      301 tagagcaaga tatgcagtaa agtgaattgc tcaccatgcg tcacaggcaa catgggcatg
      361 cccacttttg catactcttc acgacggtac agggctagtg cccagaagtg cggtggtgtc
      421 caggcaaaaa taatgaggaa catcaccaac gactcgggcg atacggtgtt attgactgcg
      481 gcccatccca aaatgggtgg catggcgcct gaggcaccgc caatgacaat attcatcggc
      541 gtggctggct tgagaaacac agtatagatg acagcatacc caacaaaggt gcccaatgtc
      601 agccacatgg tcagcggatt cacccagtag tacaaaatgc ttaagcctat accacccagc
      661 acactggcaa acagcaaagt ttccatctgt gacagctgcc cggtcggtaa cggacgtgcg
      721 cgtgtacgcg ccatcatggc atcaatttta tgctcaatca ggcagttaaa cgcggccgca
      781 gcaccggagg caaaagcaat ccccaccgtc gccgccagta aaaccgatgc cggcaccatg
      841 ccaggcgttg ccatcagcat cccaataatc gcagtaaaaa cgatcaaggc cgtcacccgc
      901 ggtttgcata aagcataaaa atgccgcagg cccattttgg ccgttgccag ttgtgtcatc
      961 caggtagttt gcatactgct attcccctta catttttaat tatgatgtct tcgtcagcct
     1021 ggcattgatt gcaaccaggc aaacgcccaa caaagctgct cctgcattat gcaacaccgc
     1081 caacaccaat ggcagctgca ataaaaggtt gcctatcccc accgcaatct ggcaaagcac
     1141 aattgccagc aatgcccacg ccagcatttt ggacgtttgg cgctgcaata gtatcctggt
     1201 gctcatcccc atccatacca acaccaccat cgcccctaac ctgtgtaccc actgaatggc
     1261 cgtcaatgca tccaagtgca actgtccacc atcacggctc acgcccaatg cacgcgtaat
     1321 atgaaaggca tcgcccatat ccatcgccgg ccaccacaaa ctgtgacagg ttggaaagtc
     1381 cgtacaagcc aaggccgcat aattactgct agtccatccg cctaacagca cttgagcgat
     1441 ccagatcagt aatgccagac gcaaccagta ctgttggtcg gccggcaagg caatatccgc
     1501 tttgcaggtg tgacgaaacg cgatccaact cagtactgcc agcgtcgtca tcccacctaa
     1561 aagatgcagg gttacgatga caggctttaa caacaaagtg actgtccaca tccccagtaa
     1621 agcctgcatg atgatcagca ccaccaacgc tacagagagt tgccaggatg tcctgattgt
     1681 aagccgttgg cgataagcca ataccgctag cccggctacc agcaaaccca agctgcctgc
     1741 cagatatctg tgcgccattt ctttccatgc tttggcatgt tcaaccggtt tatccggata
     1801 ggcctgttgc gcagactcaa tagccgcttc actttgcggt accgtcaatg cgccataaca
     1861 gcctggccag tccgggcacc ctagtcctgc atcggatagc cgcacatatg aacccagtac
     1921 gacgacacaa aaagccacga ttgcagccgt aatacttaat gctttaaaca ttgcctagcc
     1981 tgcccacgaa gcctttaaca atttgactaa atccttgcgt atccacttgg cttccatttc
     2041 cgggccatat tgcatcatga tatttccaag tgggtctatg aaatacacgc gtctggcatc
     2101 tagcgtgttt tgaccgccac cactgagcat acgacctaaa tcagtcacct gctcactctc
     2161 cccgttaatg atcagcaaat ccggatagcg ggcacgtata ggtgctgtat ccgtttgttg
     2221 ggtaatcagc acacgctgta cgcgtatcat gtctttatat aaagaaacat aaatctgtct
     2281 gaggtcatac aagcgctgca tgcatatatc atcacattca cgcgcaatca gcacgatgtt
     2341 ccacttgtcc tgccagaatg ccggcaaggc ttgctgttga tcgctgaccc aatgtgaggc
     2401 ataagcaatc gacaccggcg gttgaatcaa ctgaccataa cttttaccac ttggtcgcca
     2461 gtcaaacttg aacattgcaa ccacaaccag caacgggact gtaaaaaaaa tcgccaacag
     2521 caaaaaaatc atgcgccctt tacgctgctg cgttgggtca attgcagtta aagaatttgc
     2581 ttcctcgtgc gtcatactca agcccgtttc tccaccattt gatacacacc tatcgccaaa
     2641 cttgccaagg caaaaccaaa ccactgatag gcataactaa gatgcttctc gacctgcccg
     2701 gaaggtactt cccaatgcct cacatagcca tcgggcatcc ccgaatcgag tttaatgatg
     2761 acatcaggaa aagtatatgg cacgtgctgg cgcaaataag caaagtcaat cacaggttgt
     2821 accgcttccc atgcctggcc aggtttatcc agacggaaac ctattttggt ctgttgccag
     2881 aataagccct taatgatttt ttctgtttgc ggcgtagcga ccacaggcgt tttttggtga
     2941 tctgaaaaac ctgcgatcca gccacggttg acccagacga catgctgctg gtcttttagt
     3001 aaaaaggggg tgatcacgtg gaaaccagct tgcccttgtt ccacttggtt atcaagcaag
     3061 aaactgaact caggcaagta atgtccagtc agctcaacag tctgcaaatg caatgcttgt
     3121 acctttgccg catcatccaa gacaaatgct tgcacctgaa tggcccccgc atgatatgtc
     3181 aatgcgcgcg tgatttcctg cttttgctca gctttatgcc attgccataa accgagccga
     3241 acacaacccc acattgcggc aaccatcaca cacaccacca tccagcgtac tcgaatgaca
     3301 taatttccca agggtatttg cattttcgtg cttacagccc ataatgtaat caacttacag
     3361 cgagatcaca tcatgttcat aaaaattgcc accattttga tactgatcac cattattggc
     3421 agcttgtttt ccgccctgct tttcttgctt aaagacaaac gtggctcgga acgtaccgta
     3481 aaagccctca ccatgcgtat cggcttatct atctttctgt tccttgcttt ggtcatcgcg
     3541 gcccacttcg gctatgtggg ccacaccctc taagaaagac tagtgataaa aagggcgctt
     3601 ttagcgccct ttttattcat cagagcatgt aaacaaagat atacaaacct agccacacca
     3661 catccacaaa gtgccaatac caggccacac cttcaaagcc gaaatgatgc tcaggtgtaa
     3721 agtgtccttt gatgctacgc aaccaaatca caatcagcat taaagtacca atggtcacgt
     3781 gaaaaccgtg gaagccagtg agcataaaaa aagtggctcc gtaagcacct gatttaaggg
     3841 tcagtcccat ttcagtataa gcatgatggt attcagcggc ttggcaacac aggaaggtca
     3901 cccccagcgc aatggtcaaa aacaaaccga caattaactg cttacggtta ttcttaatca
     3961 ggccccaatg tgcccaggta atggttgcac cagagctcag cagcagcaag gtgttaatca
     4021 gcggcagtcc actccaggtc atcgggtgga atcccgtgtt gggcttgtag gtttcaccca
     4081 gcaccgtacc gcctgggcct tgcgatggcc aagtactcaa aaagtccttg tacggcgtaa
     4141 tatctggcgc ataagaagcc aaatcaggca ctgacagcac gcgcatataa aatagcgcac
     4201 caaaaaaagc tgcaaaaaaa gcaacttcgg aacagataaa tacaatcatg ccaatgcgga
     4261 aagacttgtc ttcccacttt ttgtattggc cggtaatact ttcggttacc acggtgctta
     4321 gccatttgaa catcatggtc agcacaatcg ccaaaccgat gtacatcatg tatttgccag
     4381 gggttgccag atacgataaa gccggattct tgattgagcc atccatgcgt gtatctgatc
     4441 ccgtaacatt aaaaacaaaa ccactggcca tagacagcaa accgacggaa atcagcgccg
     4501 gataaatgct gccatgcggg acaaaatagt gattttctga atgactagcc atgcattaac
     4561 tccctatcct taaattgtat tactcatatt atttattact gacttgccgg gaaaaatgaa
     4621 tacgacaaag tcatttcttg tacatcttgg ggcaagtcgg cactaataaa aaaccgaagc
     4681 ggcaatgcct tcacttcccc aggtttgagt gtctgcctga caaaacagaa acactctatt
     4741 tttttcaaat gtgccgaggc aattccaggc gtcacacttg gaattgcctg cccggccact
     4801 tcctcattgg taatgttctt tgcctcaaac at
//
LOCUS       DATMSK010000198         4990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_414766,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000198 DATMSK010000000
VERSION     DATMSK010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414766"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VNZ84_06495"
     CDS             complement(<1..390)
                     /locus_tag="VNZ84_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWU66643.1"
                     /translation="MALLQISEPGHSPAPHQHKLAVGIDLGTTNSLVATVRSGMSTVL
                     HDEHGHALLPSVVRYLDGAIVVGHEAQAAQSQDPVNTIVSVKRFMGRALHDITDRAHI
                     PYHFVENDSSQGMLELKTRAGLKSPVEI"
     gene            complement(395..907)
                     /gene="hscB"
                     /locus_tag="VNZ84_06500"
     CDS             complement(395..907)
                     /gene="hscB"
                     /locus_tag="VNZ84_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HWU66644.1"
                     /translation="MQNYFALFQLPQQFELDIAQLDNQYRKLQAEVHPDKFVSASPAE
                     RMQSMQMATLANEAYQTLKQPTARARYLLQLQGIATDEENNTAMPTDFLMAQMEWREA
                     IDDAKFSKDVPALDALLKDMRTQAKALQLQVANEIETTPTQAALTVRKLRFIDKVSED
                     VNQLIAQLED"
     gene            complement(962..1285)
                     /gene="iscA"
                     /locus_tag="VNZ84_06505"
     CDS             complement(962..1285)
                     /gene="iscA"
                     /locus_tag="VNZ84_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HWU66645.1"
                     /translation="MAITLTETAANRVEKFLANRGKGVGLRLGVKTTGCSGMAYTLEF
                     VDELLPEDVTFESHGIKVIVDPKSLVYIDGTELDFTKEGLNEGFKFKNPNVKDECGCG
                     ESFTV"
     gene            complement(1287..1670)
                     /gene="iscU"
                     /locus_tag="VNZ84_06510"
     CDS             complement(1287..1670)
                     /gene="iscU"
                     /locus_tag="VNZ84_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HWU66646.1"
                     /translation="MAYSDKVLDHYENPRNVGSMDKNDPNVGTGMVGAPACGDVMKLQ
                     IKVNDAGVIEDAKFKTYGCGSAIASSSLVTEWLKGKTLDQAQEIKNSAIAEELALPPV
                     KIHCSVLAEDAIKSAIADLKSKQGH"
     gene            complement(1849..3066)
                     /locus_tag="VNZ84_06515"
     CDS             complement(1849..3066)
                     /locus_tag="VNZ84_06515"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HWU66647.1"
                     /translation="MSERKPIYLDYSATTPVDPRVAQKMIPYLTEDFGNPASRSHPYG
                     WTAEQAVENAREEVAKLVNADPREIVWTSGATESNNLAIKGAAHFYAASKGKHIITSA
                     TEHKAVLDTVRELEREGYEATYLQPEADGLISLEKFKAAIRPDTVVASIMLVNNEIGV
                     IQDIEALGNVCRANNIIFHVDAAQATGKVAIDLEKLPVDLMSFSAHKTYGPKGIGALY
                     VRRKPRIRIEAQMHGGGHERGMRSGTLATHQIVGMGEAFRIAREEMASENERIRMLRD
                     RLLNGLLQIEETYVNGDLDHRVPHNLNVSFNFVEGESLIMAIKDIAVSSGSACTSASL
                     EPSYVLRALGRSDELAHSSIRFSIGRFTTEADVDYTIELLKSKIGKLRELSPLWEMFK
                     DGIDLSKVEWAEH"
     gene            complement(3091..4236)
                     /locus_tag="VNZ84_06520"
     CDS             complement(3091..4236)
                     /locus_tag="VNZ84_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWU66648.1"
                     /translation="MQTVFFDHNSTTALKPQVLEAMLPWLTQPTGNPTSRHVYGRNAR
                     EAMEQARKQIAECAGVQVSQVVFTSGGTEANNFAIKGITANLTPSQLLVSAIEHPCVT
                     RPAQSLAWHDWKVVQIGVDGQGVIDLRQAEQILKTRTGLVSAMLANNETGAIQPIAEL
                     ATLVKASGVLLHTDAVQAFGKIEVNMTALGVHAMTISSHKIGGPIGVGALILDKRVDI
                     APLLHGGGQERGLRSGTENVAGIVGFARACQLAMQSLDSRRLTTTALRDQLEAGLSKL
                     GAAIFAQQVTRLPNTSFFAIPNIEGETLVTALDKAGFAVASGSACSSDSTEPSHVLLA
                     MGVEPDLARGAVRVSLSDNNTSEEITQFLAALQQQVQRLKGLSAVAA"
     gene            complement(4252..4722)
                     /locus_tag="VNZ84_06525"
     CDS             complement(4252..4722)
                     /locus_tag="VNZ84_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWU66649.1"
                     /translation="MRLTTKGRFAVTAMLDIALYEADKPVTLAGISERQSISLSYLEQ
                     LFSRLRKQGLVSSVRGPGGGYRIAKAHHEISVSDIITAVDEQIDATQCGGNENCHDEG
                     RCMTHELWASLNGKILEYLSGVSLADMVDMQRKKGAGTVVFSPRKCDSEATVAA"
BASE COUNT         1150 a         1292 c         1212 g         1336 t
ORIGIN      
        1 gatctcaacc ggactcttta acccggctct ggttttgagt tccagcatgc cttgtgaact
       61 gtcgttctcc acgaaatggt aagggatgtg agccctgtcg gtgatgtcat gtaaggcacg
      121 tcccataaag cgtttgacgg acacgatggt attgaccggg tcctggcttt gcgcggcttg
      181 agcttcgtgg ccaaccacga tggcgccatc caggtaacgc acaacagaag gcagcagcgc
      241 atgaccatgt tcgtcatgca agacagtgct catgccactg cgtaccgtcg ctaccagcga
      301 gttggtggtg cccaggtcaa tgccgactgc cagtttgtgc tggtgtgggg caggggagtg
      361 tccgggttcg gaaatctgta ataaggccat taaactaatc ttccaactga gcgatcagct
      421 gattcacatc ttcactcact ttgtcgataa aacgcaactt acgtaccgtg agtgctgctt
      481 gggtgggcgt cgtctcgatt tcattggcga cttgcagttg caaagctttg gcttgcgtgc
      541 gcatgtcttt caaaagggca tccagcgcag gaacatcctt actgaatttg gcatcgtcaa
      601 tcgcttcacg ccattccatt tgtgccatta aaaaatccgt gggcatggcc gtattgtttt
      661 cttcgtctgt ggcaatgccc tggagttgta gcaggtagcg tgcacgggcg gttggctgtt
      721 tcagtgtctg gtaagcttca ttcgccagtg tggccatttg catggattgc atgcgctcgg
      781 caggcgaggc actcacaaat ttatcaggat gtacttcagc ctgtagtttg cggtattggt
      841 tatccagctg agctatgtct aactcaaatt gctgcggcag ttgaaacagt gcgaaatagt
      901 tttgcataca ctaaagaaaa ccagcataga accggggcag gcaacagcgc cccggtcagg
      961 cttagacggt aaaagactct ccgcaaccac actcgtcctt cacattcgga tttttgaact
     1021 tgaagccttc gttcaagcct tccttggtga agtccaattc tgtgccatcg atatagacca
     1081 gacttttagg gtcgacgatg accttgatgc cgtgactttc aaacgtcaca tcttctggca
     1141 gtagctcatc aacgaattcc agcgtataag ccatgcctga acagcctgtg gttttgacgc
     1201 ccaggcgtaa gccgacaccc ttgccacgat tggcgagaaa tttctcgaca cggttggccg
     1261 cggtttcagt cagggtaatt gccatattaa tgaccttgtt tggatttcag gtcagcaatc
     1321 gcagacttga ttgcatcttc tgctaatact gagcagtgaa ttttaaccgg tggcaacgcc
     1381 agctcttcgg caatcgcaga gttcttgatc tcttgcgcct gatccagcgt tttgcccttc
     1441 agccattctg tcaccagaga gcttgaagca atcgcagaac cgcaaccata ggtcttgaac
     1501 ttcgcgtctt caatcacgcc cgcgtcattc accttgattt gcagtttcat cacgtcgccg
     1561 caggctggcg cgccgaccat gccggtgccc acgttagggt cattcttgtc catagaaccc
     1621 acgttacgtg gattttcgta gtggtctagt actttatctg agtaagccat ttaacactcc
     1681 ttgaaattaa atgccatcgc aattcacgat agcttttaat ttttaattaa ctttccaatc
     1741 atccaaaaaa atgctgattt gactgtctgg caaggcggag gattgcgccg tttagttcag
     1801 ctaaacaagc attccgacaa caacgccagg cggtcaaaga ccaatttttt aatgctctgc
     1861 ccattcaact ttggacaaat caatcccatc cttgaacatc tcccataatg gggacagttc
     1921 acgtaatttt ccaatcttgc tcttcaacag ttcaatcgtg taatcgacat cggcttctgt
     1981 tgtgaagcgg ccaatgctga aacggatgga gctgtgtgcg agttcgtcag agcggcccag
     2041 ggcgcgcaac acatagctcg gttccaggct ggcagaagta caggctgaac cggaagacac
     2101 tgcaatgtcc ttgatcgcca tgatcaatga ttcgccttct acaaagttga aactcacgtt
     2161 caaattgtgt ggtacacgat gatcaagatc accattgaca taagtttctt cgatttgcag
     2221 caggccgttc agcaaacggt cacgcagcat acggatacgc tcattttcgc tcgccatttc
     2281 ttcacgcgca atgcggaagg cctcacccat gccaacaatc tggtgggtcg ccagagtacc
     2341 ggaacgcatg ccgcgttcat gaccaccacc gtgcatttgc gcttcaatac gaatacgcgg
     2401 tttgcggcgt acatacaatg cacctattcc tttcgggccg taggttttat gtgctgaaaa
     2461 gctcatcagg tctacgggca gcttttccag gtcaatcgct actttacctg tggcctgcgc
     2521 tgcatcgacg tggaaaatga tattgttggc gcggcaaaca ttgcccaggg cttcaatatc
     2581 ctggatcacg ccaatttcgt tattgaccag catgatggat gcaacaaccg tatccgggcg
     2641 aatcgctgcc ttgaattttt ccaatgaaat caggccgtcg gcttctggtt gtaggtaggt
     2701 ggcttcgtag ccttcacgct ccaactcacg caccgtatcc agcacggctt tgtgttcggt
     2761 cgccgaagtg atgatatgtt tgcctttgct tgctgcgtaa aagtgcgctg cacctttaat
     2821 ggccaggttg ttggactcgg tcgcgcctga agtccagacg atttcgcgtg ggtcagcatt
     2881 caccagtttg gccacttctt cgcgtgcatt ctccactgcc tgttctgcag tccagccata
     2941 aggatggcta cgcgaagctg ggttgccaaa gtcttcggtc aggtatggaa tcattttttg
     3001 cgcaacacgc gggtctaccg gagtggtcgc agaataatcc aggtaaatcg gctttctttc
     3061 agacatatct catgctcttt caattaaagt ctatgccgca acggcactta atcctttcag
     3121 acgctgcact tgttgctgca acgctgctaa aaactgtgtg atttcttcac tggtattgtt
     3181 atcgcttagg ctaacgcgga cagcaccacg ggccaaatct ggttctacac ccatcgctag
     3241 caatacatgg ctgggctcgg tgctatcact ggaacaggcg gagccactgg ccacggcaaa
     3301 gcccgcttta tccagcgcag tgaccaatgt ttccccctcg atattgggga tggcaaagaa
     3361 actcgtattg ggcaggcgtg ttacttgctg cgcaaagatg gccgcaccaa gcttgctgag
     3421 gccggcttcc agttgatcac gtaatgctgt cgtcgtgaga cgacgggagt ccaaggattg
     3481 cattgccaat tggcaagccc gcgcaaagcc aacaatacca gccacatttt ccgtaccact
     3541 acgtaaacca cgttcctggc cgccaccgtg taataacggt gcaatatcaa cacgtttatc
     3601 cagaatcagt gcgccaacgc caatcgggcc gccaattttg tggctggaaa tcgtcattgc
     3661 atgcacgccc aaggcggtca tgttgacttc aattttgcca aaggcctgta ccgcatcggt
     3721 atgcaacaac acgccgcttg cttttaccag ggtagccaat tccgctatcg gttgtatggc
     3781 accagtttca ttgtttgcca gcatggcaga cactaatcct gtgcgcgttt ttaagatttg
     3841 ttctgcctgc ctcaaatcta tgacaccttg accgtctacc ccaatctgga caactttcca
     3901 gtcatgccat gccagtgatt gagcagggcg ggtgacacat gggtgttcaa tcgcactgac
     3961 cagtagctgt gatggtgtca ggttggccgt gatgcctttg atggcaaagt tattcgcttc
     4021 ggttccgcca gaagtgaata cgacttgcga aacctgtaca cctgcgcatt cggcaatctg
     4081 ctttctggct tgttccatgg cttcacgcgc attgcggcca tacacatgcc gactggtcgg
     4141 gttgccagtt ggttgcgtca accaaggcaa catcgcttcc aatacctgtg gtttcaaagc
     4201 ggtggtgcta ttgtggtcaa agaaaacggt ttgcatggtc atgcccaaga gctatgcagc
     4261 gactgttgcc tcagaatcac attttcttgg cgaaaatacg acggtcccag cccctttttt
     4321 gcgttgcatg tctaccatgt ctgccaggct gacccctgat agatattcaa ggattttgcc
     4381 attcaacgat gcccataatt catgcgtcat gcaacggcct tcatcatggc aattttcatt
     4441 accaccacac tgtgtggcat caatttgctc atccacggcg gtgataatgt cagaaactga
     4501 aatctcatga tgagctttgg caatgcgata gccgccaccg gggccgcgca cgctggaaac
     4561 caggccttgt ttgcgcaagc ggctaaacaa ttgctcaagg taagaaaggg aaatgctttg
     4621 acgctcgctg ataccagcca atgtgactgg cttatctgcc tcgtaaagtg caatgtccag
     4681 cattgcagtc acagcaaaac gtcctttggt agttaatcgc atctcaagat gaccttttaa
     4741 aacagaggtg aataatataa taacctactg ttttagtcaa ttattatgac cgaaaagttc
     4801 aggccaatat ggtaagtgat cttgatgagg attattcagg cttttttgca aaagcttcgc
     4861 cgacgccgct ctctgtttca gcgtcactga gtttggctaa ttgcactttc agttcggcga
     4921 tctcggcttc ctgcttatgc gcatgatcaa gcaggctgtt aatggctttg atcatggggt
     4981 cattttcatc
//
LOCUS       DATMSK010000199        16244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_38670,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000199 DATMSK010000000
VERSION     DATMSK010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 16244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16244
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38670"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /gene="pbpG"
                     /locus_tag="VNZ84_06530"
     CDS             complement(<1..1061)
                     /gene="pbpG"
                     /locus_tag="VNZ84_06530"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HWU66650.1"
                     /translation="MCSIAFAPAGQAIAKSPSKKQTVSTAKSKKYSTRASSQYRVNPE
                     KTKSMRPVKMHTHARKSARPAVDAEMFDSFQGKSSSNGNLSIASTKALVMNQNTHEII
                     YSKNLDTPTPIASVTKLMTAMVVLDAKLNLNEQVSITEMDVDYLKGTSSRLPVGTTMT
                     REDMLNLALIASENRAASALATNYPGGKARFIQDMNAKAASLGMMNTHFEDSTGLTSN
                     NVSTAMDLAKMVHAAYQYPLIRQITTTSDYDLSVGRRQPLHFHNTNALVRDSSNSSWE
                     IGLSKTGYISEAGRCLVMQATIAGEPLILVLLDSVGKLTRIGDAKRIKKWMEHNYNTL
                     SSNSGADGEIVLTGLSMSKT"
     gene            1277..1648
                     /locus_tag="VNZ84_06535"
     CDS             1277..1648
                     /locus_tag="VNZ84_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system H protein"
                     /protein_id="HWU66651.1"
                     /translation="MSAGLKYFFSKDHTWAAQSDDGCWLVGITDYAQNMLGDLVFVDP
                     PKVGQAVEQFTVCGLVESVKTGSDIYAPLSGEVQAVNEEALASPEKINDHPYETWLFK
                     LAAGASGVDSLLDQTAYEAQL"
     gene            complement(1678..1872)
                     /locus_tag="VNZ84_06540"
     CDS             complement(1678..1872)
                     /locus_tag="VNZ84_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66652.1"
                     /translation="MQQFSLKISFMLTALFFLGFASRDLNIHHTTLSLGNHAGSGKVV
                     TLQLADLLYAEPTLKIKRAQ"
     gene            2001..2264
                     /locus_tag="VNZ84_06545"
     CDS             2001..2264
                     /locus_tag="VNZ84_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWU66653.1"
                     /translation="MPIYDFQCTACGHQQELMRKVSAPSVEACPKCQQQTFSKQVSAP
                     NFQLTGSGWYATDFKNNQAASSKPAETASAGESAKCNPGCACH"
     gene            2277..2876
                     /locus_tag="VNZ84_06550"
     CDS             2277..2876
                     /locus_tag="VNZ84_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HWU66654.1"
                     /translation="MKKYFITGLLVLVPLFITVWVLKTLVQTLDQSLLLLPAAWRPEA
                     WLGIDIPGFGVILTVGIVLATGLVATNIFGQQLIELWEGLLVRVPVVKSIYSSVKQVS
                     DTLFSDSGNAFRQALLIEYPRQGSWTIAFLTGTPGGDVANHLQGEYVSVYVPTTPNPT
                     SGFFLMLPRSEVIELDMSVDQALKYIISMGTVAPLPKHH"
     gene            2909..4699
                     /gene="aspS"
                     /locus_tag="VNZ84_06555"
     CDS             2909..4699
                     /gene="aspS"
                     /locus_tag="VNZ84_06555"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWU66655.1"
                     /translation="MMRTHYCGHLNREHIGQTVTLCGWAHRRRDHGGVIFIDLRDREG
                     LAQIVIDPDTKDAFAIAETVRSEFVLRVVCKVRARPEGTVNPNLPTGEVEMLATEIEV
                     LNASLTPPFMLDDDNLTETVRLEHRYIDLRRPAMQKNLMLRYKVAKNLRDYLDENGFI
                     EVETPMLTRSTPEGARDYLVPSRVHHGMFFALPQSPQLFKQLLMVSGFDRYFQITKCF
                     RDEDLRADRQPEFTQVDIETSFLEENEIMDIVEEMIRRMLKKVQNVDLPAKFPRMPFS
                     EAMNRYGSDKPDMRVTLEITELSDVMKDVDFKVFAGAANMAGGRVAAIRVPNGAAISR
                     SEIDAYTEFVKIYGAKGLAYIKINDITKLNEEGLQSPIVKNIHVNALQAIIERTGAQN
                     GDIVFFGADKAKIVNEALGALRTKVGHEKGHVDGRQWAPLWVVDFPMFEHDEEADRWV
                     ALHHPFTAPKDGHEDLLVSNPGACLSKAYDMVLNGWEVGGGSVRIHKQDIQSKVFDAL
                     KISKDEAQEKFGFLLDALQYGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKTQRAQ
                     CLMTNAPNEVDEKQLRDLHIRVRQQNVAGS"
     gene            4800..5330
                     /locus_tag="VNZ84_06560"
     CDS             4800..5330
                     /locus_tag="VNZ84_06560"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWU66656.1"
                     /translation="MIDLTAYVRTIADYPKAGIQFRDITTLLKDAVAFNAAIDALALR
                     YADVALDKVVGIESRGFIIGAALASKLSLGFVPVRKPGKLPGASISQVYALEYGQDAL
                     HLHTDAIAAGEKILLIDDLIATGGTALAAVALIRQLGGMVEHAGFVIDLPALAGMQRL
                     QNIGVQTFALCQFEGH"
     gene            5399..5827
                     /locus_tag="VNZ84_06565"
     CDS             5399..5827
                     /locus_tag="VNZ84_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU66657.1"
                     /translation="MPLTGIDHVNFRTDRDTMHQLRDFYCDVLGLTVGKRVASTTYGY
                     WLYIGKQAVVHLAEYKTAEAPQLHVHGTYDHVSFNCNDMPAMEAHLQKLGVTYTTRIL
                     ANGIRQINMRDPAGNGVELNFAEYADPDYVMRPSGDPSKL"
     gene            5925..6713
                     /locus_tag="VNZ84_06570"
     CDS             5925..6713
                     /locus_tag="VNZ84_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HWU66658.1"
                     /translation="MKKVWMMMLGVMALGMAGQAYAEADPKLWAVVKEAFFPKREIQE
                     VDFLKIEAPRRAESGAQVPVTLIYDKAAANGVELKKLYVIVDANPIQLASTYHLTDML
                     NGFQMATRIRQETDSFVRLIGETADGKLYMGKREIRAAGGCGGTVDNNEAEVRAAAGK
                     IKLNVDAPKFGEPATATFNIKHVMRTGLQRDLVSQGYVPAFYINKTTFTYNGKEVMTV
                     DVGVGTSEDPYMKFSFIPEAPGKLEVVATDNEGKTFTHSLDVHG"
     gene            6817..7236
                     /locus_tag="VNZ84_06575"
     CDS             6817..7236
                     /locus_tag="VNZ84_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWU66659.1"
                     /translation="MKSFRKELWFNIPARMDFKNITHDVMACVRESGVKEGLVLINAM
                     HISASVFINDDERGLHHDYKVWLEKLAPHEPVSGYRHNDTGEDNADAHIKRQIMGREV
                     VVAITDGQLDFGPWEQIFYGEFDGRRNKRVLVKIIGE"
     gene            7244..8590
                     /gene="yegQ"
                     /locus_tag="VNZ84_06580"
     CDS             7244..8590
                     /gene="yegQ"
                     /locus_tag="VNZ84_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HWU66660.1"
                     /translation="MKSPELLAPAGDLDRMRTAFDYGADAVYAGQPRYSLRVRENDFS
                     MQNLAIGIQEAHARGKKFFVASNISPHNAKVKTYMRDMAPVIDMQPDALIMADPGLIM
                     MVREQWPEMPIHLSVQANAVNYASVKFWQRMGLTRVILSRELSLDEIAEIRQECPDME
                     LEVFIHGALCIAYSGRCLLSGYFNSRDPNQGTCTNSCRWDYKVHDAQPDAAGVIRLKE
                     FDFHAEMEKSNLSSCGSLPRHPAADDVYLIEEKGRPGELLPIMEDEHGTYIMNSKDLR
                     AIEHVEKLIAIGVDSLKIEGRTKSRYYVARTSQNYRQAIDDAVAGRPFDWRLLGELEN
                     LANRGYTDGFYQRHHTAEHQNYVQGHSSANRSLYVGDVVDYDAESGLASIQARNKFAV
                     GDKLQLIHPKGNRDIVVEQLFDKRGEPIEQAAGSGHFVKLPIAAAALNNAMVARYL"
     gene            complement(8658..9089)
                     /locus_tag="VNZ84_06585"
     CDS             complement(8658..9089)
                     /locus_tag="VNZ84_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWU66661.1"
                     /translation="MKTLQQVLNLKTHQGIISIAPGRPVYDALVILAEYKIGALAIIK
                     DGELVGIFSERDYAREVILKGRSSKTTPISDVMTKKVITGKPSDLVESAMSTMLEKRI
                     RHLPVVDNGKMLGMLSIGDILKETISYQQELIRQLESYIHS"
     gene            9200..9712
                     /locus_tag="VNZ84_06590"
     CDS             9200..9712
                     /locus_tag="VNZ84_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU66662.1"
                     /translation="MLQLRDLPTQEVLERFAQRYPEADVTAIASFLMLLRVATDLSVA
                     LDACLSKHDLLQGRWWVLILLMREVDLTSTPSVLAEKLGITRATMTGLLDGLEQSGLV
                     QRVSAPHDRRSVQIKLTEVGQAKLDAVMPDYYTRLRLCMQGLSESQRNDLQSILGVID
                     QGISAWDFNQ"
     gene            9742..10119
                     /locus_tag="VNZ84_06595"
     CDS             9742..10119
                     /locus_tag="VNZ84_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HWU66663.1"
                     /translation="MATVVDTLSALNQQFDAAFNSKNTQAIAALYANDAIVMPAPAGQ
                     VVKGRQAVADFFGGLIEAGVIEHALTMKEAVVSDTMATQTGNWAAAMIGEDGVKQQFS
                     GNVQVVFQKQSDGQWLAVTHIWN"
     gene            10153..11055
                     /locus_tag="VNZ84_06600"
     CDS             10153..11055
                     /locus_tag="VNZ84_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWU66664.1"
                     /translation="MDQLIKQRSQALTALLVLTIIWGYNWVVMKSALQYAGPFQFAAM
                     RTFFGALVLFLVLYVTRRPMGLTEFPTMLLLGILQNVGFTGVLMWALVEGGAGKTAVL
                     TYTMPFWTLLFAWPMLGEHVHGWQWLAVIAAVLGMLFIFDPLHIRADSFSMLLAVLSG
                     VSWALSAIMTKQLHRRHPTLDLINLTAWPMLLGSLPMVLIAWLVPAPPIDWSPYLIGA
                     VLFNVVLCGALAWLLWLFALQRLPAGVASMASMLAPVIGVIAAWIQLNEVPDHMEMTG
                     MALIIFSLSLISFLMMRPGYGKSQ"
     gene            11199..11924
                     /locus_tag="VNZ84_06605"
     CDS             11199..11924
                     /locus_tag="VNZ84_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU66665.1"
                     /translation="MAGHSKWANIQHRKGRQDAKRGKIFTKLIKEITVSARMGGGDTA
                     MNPRLRAAVAAAKEENMPSDNITRAIKKGTGELEGVNYEEIRYEGYGINGAAIIIDCL
                     TDNRQRAVMDVRHALTKHGGNLGTDGSVAFMFKHCGQLVFEPGVSEDAVMEVALEAGA
                     EDVITDEDGSIEVITAPGDFVTVKEALEAAGLKAVMAQVTMKSLNEVVFTGDDAVKMQ
                     KILDMLEDLDDVQDVYTSAVIEE"
     gene            11957..12517
                     /gene="ruvC"
                     /locus_tag="VNZ84_06610"
     CDS             11957..12517
                     /gene="ruvC"
                     /locus_tag="VNZ84_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWU66666.1"
                     /translation="MAAQRILGIDPGLRITGFGVIEAVDGKLSYITSGVIKTASAKKT
                     AKGESEKEALPERLKVILDGLFEVIATYQPTQVAIEKVFVNVNPQSTLLLGQARGAAI
                     SAAVIRQLSVAEYTALQVKQSVVGNGHAQKEQVQEMVKRLLKLPANPSPDSADALACA
                     ICHAHGGQGLGALATTGFRVRNGRLI"
     gene            12514..13089
                     /gene="ruvA"
                     /locus_tag="VNZ84_06615"
     CDS             12514..13089
                     /gene="ruvA"
                     /locus_tag="VNZ84_06615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWU66667.1"
                     /translation="MIARLQGKLLEKSPPLIVVDCNGVGDEVEVPMSTFYNLPELGQS
                     VQILTHMVVREDAQLLYGFGSEQEKNTFKQLLKVNGIGAKSALSILSGVSVNDLMLAV
                     SQQEVSMLTRIPGIGKKTAERLLLELKDKFVVTGSTSAQPAAKAATDDILNALVALGY
                     HEREAGATVKLLEPGVSVSDGIRQALKYLAK"
     gene            13222..14265
                     /gene="ruvB"
                     /locus_tag="VNZ84_06620"
     CDS             13222..14265
                     /gene="ruvB"
                     /locus_tag="VNZ84_06620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWU66668.1"
                     /translation="MIETDRLVAPATASAQEEALERALRPKLLDEYVGQEKARGQLEI
                     FINAARGRQEALDHVLLFGPPGLGKTTLAHIVAKEMGVNLRMTSGPVLERAGDLAALL
                     TNLEPNDVLFIDEIHRLSPVVEEILYPAMEDYRLDIMIGEGPAARSVRLDLPPFTLVG
                     ATTRAGMLTNPLRDRFGIVARLEFYSHEELAKIVSRSANLLEVDMQSAGALEIARRAR
                     GTPRIANRLLRRVRDYAQVKSDGIVTDVIADAALKMLDVDQQGLDVMDRKLLQAVVEK
                     FGGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGYLMRTPRGRVATMLTYQHFGLKPP
                     ASMVSGDLWQDGQ"
     gene            14267..14662
                     /gene="ybgC"
                     /locus_tag="VNZ84_06625"
     CDS             14267..14662
                     /gene="ybgC"
                     /locus_tag="VNZ84_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HWU66669.1"
                     /translation="MFVFPVRVYYEDTDAGGVVYHSRYLNFMERARTEWLRAAGFVQT
                     VLKQQEQVLFVVHSMQLQFKKPARLDEELKVVSQLIEMGRGSFSCRQQIIRDETVLIE
                     AQVKVACVDADSFKPTGIPARIKIALESS"
     gene            14659..15333
                     /gene="tolQ"
                     /locus_tag="VNZ84_06630"
     CDS             14659..15333
                     /gene="tolQ"
                     /locus_tag="VNZ84_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HWU66670.1"
                     /translation="MNVTNDMSIFTLVSGASLPVQLVMLILLLTSLVSWWYIFIKVFS
                     LKAANENVDQFEAQFWSGGDLNELYERITAGRKTPQGMASIFQAGLKEFIRYKQQKDT
                     EVSDVMEATRRAMRAAYNRELDYLDSHLPFLASVGSVSPYIGLFGTVWGIMNAFRGLS
                     NVAQATLSQVAPGIAEALVATAIGLFAAIPAVIAYNRFAAGVDRLSVRYESFIEEFTN
                     ILQRRA"
     gene            15345..15737
                     /locus_tag="VNZ84_06635"
     CDS             15345..15737
                     /locus_tag="VNZ84_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HWU66671.1"
                     /translation="MSRTRKRRMMNQINVVPYIDVTLVLLVIFMVTAPMTNPGMVELP
                     KVGQALKQSGDPIVVTVQKEGTVMMDGKEMQRDDMLALIRQRLSEAEKPVVVSADKNT
                     PYGEVVSVMDLLKQAQVDKVGLLFQNEK"
     gene            15803..>16244
                     /gene="tolA"
                     /locus_tag="VNZ84_06640"
     CDS             15803..>16244
                     /gene="tolA"
                     /locus_tag="VNZ84_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HWU66672.1"
                     /translation="MSPQDQAIRKRAAVLSVLMHVLLFLGMIFMIDWKAVHVNPSVME
                     ATLWDSLPSKQPVKPVTKPVDPTPPPPAPEPKPEPKPPEPKPPQPEPKPDPQVKQAEQ
                     EAQIALKKQEDEKKRQLEEEKKKEEEKKKQEEKLKKIQEALRKQE"
BASE COUNT         3944 a         3603 c         4512 g         4185 t
ORIGIN      
        1 gttttgctca tggataatcc tgtcagcaca atttcaccgt ctgccccact attggatgac
       61 agggtgttgt agttgtgttc catccatttc ttgatgcgct tggcatcgcc gatacgggta
      121 agttttccca cagaatcgag tagtaccagg attagtggtt cgcccgcgat ggtggcttgc
      181 atcacgaggc aacggcccgc ttcgctgata tagccagttt tagacaggcc gatttcccag
      241 ctactgttgc tgctgtcgcg caccaaagca ttggtgttat ggaagtgcaa tggctggcgc
      301 ctgcccacac tcaagtcata atcagatgtg gtggtgatct ggcgaatcag tggatactgg
      361 tatgcagcat gcaccatttt ggccaggtcc attgcagtgg agacattgtt gctggtcagt
      421 ccggtggagt cttcaaagtg ggtgttcatc atccccaggc tggctgcttt ggcattcatg
      481 tcttgaatga agcgtgcttt gccaccaggg taattggttg cgagtgccga agctgcacgg
      541 ttttcagagg cgattaatgc caggtttagc atgtcttcac gtgtcatcgt ggtgccgacc
      601 ggcaggcgtg aggaggtgcc tttaaggtag tccacgtcca tttcggtaat ggagacctgc
      661 tcatttaaat taagcttggc atccagtact accatggcgg tcatcagttt tgtcactgaa
      721 gcaatcggtg ttggggtatc caggttcttg gagtagatga tttcatgtgt gttctgattc
      781 atcaccaggg ctttggtgga ggcaatcgag agattgccgt tgctgctgga tttgccttgg
      841 aacgagtcga acatttcggc atcaacggct ggcctggcag actttctggc atgcgtatgc
      901 atcttgactg gacgcatgga ttttgttttc tcgggattca cacggtattg tgaagaggct
      961 cgcgtgctat attttttact tttcgctgta gagaccgtct gcttcttgga aggtgacttt
     1021 gcaatggctt gacccgcagg tgcaaaagca atggaacaca ttagcaatac gttaagacaa
     1081 tgcttgtaga aactcataat caaccttccg aaaacccatt tcattattgc ccagttatta
     1141 ttcagagtca atcacttatc gcaatttttt agtgtaaaaa attaagcctt ttctaaggct
     1201 gttttcaata agttttggtt gcattcacag actgtggcac aataattatc tactaaactt
     1261 ttgattttta aatcatatgt cagcaggctt gaaatacttc ttttctaaag accacacttg
     1321 ggcagcgcaa tctgacgatg gctgctggct ggttgggatt actgattacg cccaaaatat
     1381 gttgggagac ctggtgtttg tggatccgcc taaagttggt caagcggtgg agcagtttac
     1441 cgtgtgcggg ttggtcgagt cggtgaaaac gggctcggat atctatgcgc cactatctgg
     1501 tgaagtgcag gccgtcaatg aagaggcgct ggcttcacct gaaaaaatca atgaccatcc
     1561 ctacgagacc tggttgttca agttggcagc aggtgcaagc ggtgtggatt cattactgga
     1621 ccagactgcg tacgaagcac aactctaatg tcataccgca ttgcgggagt tctcctgtta
     1681 ttgcgctctt ttgattttga gtgttggttc tgcatagagt aaatctgcca gttgtaaagt
     1741 taccactttg cctgatccgg catgattccc cagcgataga gtggtgtggt ggatattcag
     1801 gtcgcggctg gcaaacccca gaaaaaacag cgcagtcagc atgaaagata tcttgagtga
     1861 aaattgttgc atgtcgtgct ccttaaagtc ttttgaattc aaatacatcg cggctgtttt
     1921 tatgatttgc aacgcgaatc aggtataatt tgcttttact aaaaattttc agcaaattgt
     1981 tcttcgggtt gtgttaacaa atgcctatct acgatttcca atgtaccgca tgtggtcacc
     2041 agcaagagct gatgcgcaag gtctctgcgc cttctgtaga ggcttgccct aaatgccagc
     2101 agcaaacttt tagcaagcag gtctcagcgc ctaacttcca actgacgggt agtggctggt
     2161 atgcgactga tttcaaaaac aatcaggcag caagcagtaa gccggctgag actgccagtg
     2221 ctggtgaatc agccaagtgc aatcccggtt gtgcctgcca ctaaaaaacg atccccatga
     2281 aaaaatattt catcacaggc ttgctggtat tagtcccatt attcattacg gtgtgggtgc
     2341 tcaaaacttt agtacaaacg ctggaccaga gcctgctgct gttgccggct gcctggcgcc
     2401 ctgaggcatg gttgggaatt gatattcctg gctttggcgt gatcctgacg gttgggatcg
     2461 tgctggcaac agggttggtg gcgacaaata tttttgggca gcaattgatt gagttgtggg
     2521 aggggctgct ggtacgtgtg cctgtagtca aatctatcta ttccagtgtc aagcaagtgt
     2581 ccgataccct gttctctgac tctggtaatg ctttccgtca ggccttgctg attgaatatc
     2641 cccggcaagg ttcatggacg attgcctttt taaccggtac gccgggcggc gatgtcgcta
     2701 atcacttaca gggcgaatat gtgagtgttt atgtcccgac cacgcctaat ccgacttcgg
     2761 gtttcttttt gatgttgccc aggtcggaag tgattgagct tgatatgagt gtggatcagg
     2821 ccttgaaata catcatcagc atgggtacag tggcgccctt gcctaaacac cattaaccgc
     2881 aacacacctt taactctata aaattattat gatgcgtaca cattattgcg gtcacctgaa
     2941 ccgcgaacat attggacaaa ctgtgacact gtgtggctgg gcccaccgtc gtcgcgacca
     3001 cgggggcgtg atttttattg atttgcgtga tcgcgaaggc ctggcgcaaa tcgtgattga
     3061 cccggatacc aaagacgcat ttgcgattgc tgaaacggta cgtagtgagt ttgtcttgcg
     3121 tgtggtctgt aaagtacgtg cccgtcctga aggtactgta aaccccaacc tgcctaccgg
     3181 tgaggttgaa atgttggcga ctgaaattga agtactgaat gcttcattga cgccgccgtt
     3241 catgctggat gacgacaacc tgaccgagac ggtgcgtctg gaacaccgtt atatcgatct
     3301 gcgccgtcct gccatgcaga aaaacctcat gctgcgctac aaagtagcga agaacctgcg
     3361 cgactatctg gatgaaaacg gcttcattga agttgaaaca ccaatgctga cacgttcaac
     3421 acctgagggt gcacgtgatt acctggtgcc atcccgtgta catcacggta tgttctttgc
     3481 actgccgcaa tcaccacaat tgttcaaaca gttgttgatg gtgtccggtt ttgaccgcta
     3541 tttccagatc accaagtgtt tccgtgatga ggacttgcgc gccgaccgcc agccagaatt
     3601 tacccaggtc gatattgaaa catcgttcct ggaagaaaat gaaatcatgg acatcgttga
     3661 agagatgatc cgccgcatgc tgaaaaaagt gcaaaacgtc gatttgccag ccaagttccc
     3721 acgcatgcca ttttctgagg cgatgaaccg ttatggttcc gataagccgg atatgcgcgt
     3781 gacactggaa atcacagagc tgagcgatgt gatgaaagat gtagacttta aagtgtttgc
     3841 cggtgctgcc aatatggcag gtggccgtgt ggcggcgatc cgcgtgccta atggggcagc
     3901 gattagccgc tctgagattg atgcttacac cgagtttgtg aaaatctacg gtgccaaagg
     3961 tctggcctat atcaagatca acgacattac caagctgaac gaagaaggcc tgcaaagccc
     4021 gattgtgaag aacattcacg tcaacgcgct gcaagccatt attgagcgta ctggcgcaca
     4081 aaacggtgat atcgtattct ttggtgcaga caaagccaaa attgtgaacg aagcgctggg
     4141 tgccttgcgt accaaagtcg gccacgaaaa aggccatgtc gatggtcgcc agtgggcgcc
     4201 attgtgggtg gtggattttc cgatgtttga gcatgatgaa gaggctgacc gctgggttgc
     4261 cctgcatcac ccgttcaccg cgcccaaaga tggtcacgaa gatttactgg tcagcaatcc
     4321 tggcgcctgc ttgtccaaag cctatgacat ggtgctaaac ggctgggaag tgggtggcgg
     4381 ttccgtgcgt atccacaaac aggacatcca gtctaaagtg tttgatgcat tgaaaatcag
     4441 caaggacgaa gcgcaagaga agtttggctt cctgctggat gccttgcagt acggtgcgcc
     4501 tccacatggt ggtctggcgt ttggcctgga tcgcctggta accttgatga caggtgccga
     4561 gtctatccgt gacgtgattg ctttcccgaa aacccagcga gctcagtgtc tgatgaccaa
     4621 tgcgccaaac gaggtggacg agaagcagct gcgcgatttg catatccgcg tacgccaaca
     4681 gaacgtcgct ggtagttagg acgactggct tgtgtgacag gcctgtgtga ttgcattaaa
     4741 cgcgtctgcc aatgggtagg cgcgtttttt attggcgtaa aggtttattg ggctggagta
     4801 tgatcgactt aactgcttat gtgcgtacca ttgcagatta tcctaaggct ggtatccagt
     4861 ttagggacat cacgaccctg ctcaaggacg ctgttgcttt taatgcggct attgatgcac
     4921 ttgccttgcg gtatgcggat gttgccctgg acaaagtggt tggcatcgag tcgcgcggat
     4981 ttattatcgg tgcggcactg gcgagcaagc tgagtttggg atttgtccct gtacgtaagc
     5041 cgggcaagtt gccaggtgcc agcatttccc aggtgtatgc gctggagtat gggcaagatg
     5101 ccttacactt gcacaccgat gcaattgcgg caggtgaaaa gatcttgctc atagatgact
     5161 tgatcgcaac cgggggcact gccctggcag cggttgccct gatccggcaa ttgggcggca
     5221 tggttgaaca tgccgggttt gtgattgatt tgccggcgct ggcgggtatg caacgattgc
     5281 aaaatatcgg cgtccaaaca tttgccttgt gtcaatttga gggacattag tttttgtact
     5341 atattgcgca ggttatctgc acctattttt tatacggcac tcatcagatt ggaaaatcat
     5401 gccattgaca ggaattgatc acgttaactt tcgcactgac cgcgacacca tgcatcagtt
     5461 gcgtgatttt tattgcgacg tgttgggctt gacggtaggt aaacgggtgg ctagcactac
     5521 ctatggctac tggctatata ttggcaagca agcagtggtg catctggcag aatacaaaac
     5581 ggctgaagcc ccgcaattac atgtgcatgg cacctatgat catgtttcct tcaactgtaa
     5641 cgacatgccg gcgatggagg cacatctgca aaagttgggc gtgacctata ccacgcgtat
     5701 cctggcgaac ggtatccgcc aaatcaatat gcgtgacccg gcgggtaacg gcgttgagct
     5761 gaactttgct gaatatgcgg atccagacta cgtcatgcgg ccttcgggtg acccgagcaa
     5821 gctttgattg gtattgtgat gtgcatcaca tattgtcgtc aggcattcgt tagacaatta
     5881 ttttcacgca gatcaaaatt aattatttga gtacggggaa gatcatgaaa aaagtatgga
     5941 tgatgatgtt gggggtgatg gcattgggca tggccggaca ggcatatgcg gaagccgatc
     6001 ccaagttatg ggcagtggtc aaagaggcat tttttcccaa gcgtgagatt caggaggttg
     6061 atttcctgaa aattgaagcg ccgcgccgcg cggaaagtgg tgcgcaagtg ccggtgacgc
     6121 tgatttatga caaggccgcc gccaatggcg tggagcttaa aaaactgtac gtgattgtgg
     6181 atgctaaccc gattcaactg gcatctacct atcatctgac cgacatgctg aatggttttc
     6241 agatggccac ccgtatccgc caggaaacgg attcttttgt caggttaatt ggtgaaacag
     6301 ctgatggcaa actgtatatg ggtaaacgtg aaatccgtgc cgccggcggc tgtggtggta
     6361 cggtagataa caacgaagct gaagtacggg cagccgcagg caagatcaag ctgaatgtcg
     6421 atgcacccaa gtttggtgaa ccagccacag ccacgtttaa tatcaaacat gtgatgcgca
     6481 caggtttgca gcgcgacctg gtttctcagg gatatgtacc agcgttttat atcaataaaa
     6541 cgacttttac ctataacggt aaagaggtga tgactgtgga tgtgggtgta ggaaccagtg
     6601 aggatcctta catgaagttc agctttattc cagaggcccc tggcaaactg gaggtggtcg
     6661 ctactgataa tgaaggtaaa acatttacac atagcctgga tgtgcatggc tgagatgccg
     6721 ttgacagaca aggcctcttt ggaggccttt tttgttttgg ggcaaatgcc tggttgaaca
     6781 cggtgctaaa ataacttctt tcggaaaggg gatgagatga aaagttttcg taaggagttg
     6841 tggttcaata ttccggcgcg tatggatttc aagaacatta cgcatgacgt gatggcttgc
     6901 gtacgtgaga gtggcgtgaa agaagggctg gtattaatca atgccatgca tatttctgcc
     6961 agtgtattta tcaatgacga cgagcgcggc ctgcatcacg actacaaagt ctggctggaa
     7021 aaattggcac cacatgagcc tgtgagtgga taccgtcata acgataccgg cgaggataat
     7081 gccgatgcgc atatcaagcg ccagatcatg ggtcgtgaag tggtggtggc gattaccgac
     7141 ggccaactgg actttggccc ttgggaacaa attttttatg gtgaatttga cggtcgccgc
     7201 aataaacgtg tgttggtgaa aattatcggt gaataaattg agtatgaagt ctcctgaatt
     7261 gttggcccct gccggtgacc ttgatcgcat gcgtacagcg tttgattatg gggcggatgc
     7321 tgtttatgct ggccagccgc gctatagctt gcgtgtacgt gaaaatgatt tttccatgca
     7381 gaatctggcc atcggtattc aggaggcgca tgcgcgcggc aaaaagtttt ttgtcgccag
     7441 taatatttca ccgcataacg ccaaggtgaa aacctatatg cgcgatatgg cgccagtgat
     7501 tgatatgcag ccggatgcat tgatcatggc cgatcctggc ctgattatga tggtgcgcga
     7561 gcaatggccg gagatgccga ttcatttatc cgtgcaggcc aatgccgtca attacgcatc
     7621 agtcaaattc tggcagcgca tggggctgac acgcgtcatt ttgtcgcgtg aattgtcctt
     7681 ggacgaaatt gccgagatcc gccaggaatg tccggacatg gagctggaag tctttattca
     7741 tggcgcctta tgtattgcct actcaggacg ttgcctgttg tctggttatt tcaattcgcg
     7801 ggatcccaat cagggcactt gtaccaacag ttgccgttgg gattacaaag tgcacgatgc
     7861 ccagcctgat gctgcaggcg tgatccggct gaaagaattc gatttccatg ctgaaatgga
     7921 aaaatccaat ttatcttcct gtggatcatt gccgcgccat ccggcagcag atgatgtcta
     7981 cctgatagag gaaaagggtc gccccggcga gttgctaccg atcatggaag acgagcatgg
     8041 tacctatatc atgaacagca aggatttgcg cgcgattgaa cacgtggaga agttgattgc
     8101 gattggcgtc gattcgctca aaatcgaagg acgtaccaaa tctagatact atgtcgcgcg
     8161 taccagccag aattaccgcc aggcgattga cgatgcggta gcgggcagac cgtttgactg
     8221 gcgtttgctg ggtgaactgg aaaacctggc taaccgtgga tataccgatg gtttttatca
     8281 gcgccaccat accgctgagc atcagaatta tgtgcaaggg cactccagtg ccaaccgcag
     8341 tttatatgtc ggtgatgtgg tggattacga tgccgaaagt ggcttggctt caatacaggc
     8401 acgtaacaaa tttgcagtag gggacaagct gcaattgatt cacccgaaag gaaatcgcga
     8461 tattgtggtg gaacaattgt ttgataaacg aggagagccg atagagcagg ctgcaggcag
     8521 tggacatttt gtaaagctac cgattgctgc agcggcactc aataacgcca tggtcgccag
     8581 gtatttataa acgttctgat caaaaataaa gggaagctaa gctccccttt atttttatcc
     8641 tgatggacgg gaaggtttta actgtgaatg tagctttcca attgcctgat cagttcttgc
     8701 tggtaactga tcgtttcttt caggatatcc ccgatagata acatccctag catcttgcca
     8761 ttgtcgacga cgggcagatg gcgaatacgt ttttccagca tcgtactcat ggccgactcc
     8821 accagatctg aaggcttacc ggtgatgacc tttttagtca tgacatcgct gataggtgtt
     8881 gtttttgatg agcggccttt gagaatcact tcccgcgcat agtcgcgctc agagaaaatg
     8941 cctaccagct cgccgtcttt gatgatggcc aatgcaccga tcttgtattc tgccaggatc
     9001 acaagtgcat cgtataccgg tctgcccggt gcaatgctga taataccttg gtgtgttttc
     9061 agattgagta cttgttgaag tgtcttcatc actgcctcct ttcatgatca cagaccccta
     9121 atatacaccc atgcgatgta ttagtaacat atttgtttca cttgacataa ttagccagct
     9181 aactataatg tagctaatta tgttgcagct tagagatctc cccacccaag aagtcctgga
     9241 aaggtttgcc cagcggtatc ccgaggcaga cgtgacagct attgccagct tcttgatgtt
     9301 gttgcgcgtg gccacagact tgtctgttgc gctggatgca tgtttgagta agcacgactt
     9361 gctgcaagga cgatggtggg tgttgattct gttgatgcgt gaggtggatc tgacctctac
     9421 gccatctgtg ttggccgaaa agttagggat cacgcgtgca accatgaccg gattgctaga
     9481 tggtctggag cagagtggcc tggtacaaag ggtttctgca ccacatgata gacgcagtgt
     9541 gcaaatcaaa ctgaccgaag tgggccaggc caagctggat gcagtgatgc ccgattatta
     9601 cacgcgcctg cgtttatgca tgcaagggtt atcggaaagt cagcgtaacg atctgcaaag
     9661 tattttaggt gtgatagacc aagggatatc cgcctgggat tttaatcaat aatttcgaaa
     9721 ttcttaaaga ggagtaacac catggcaaca gtcgtcgata ctttgagcgc gttaaatcaa
     9781 caatttgatg cggcgtttaa cagcaaaaat acacaggcaa ttgcggcctt gtacgccaat
     9841 gacgcaattg tgatgccggc accggccggt caagtagtta aaggccgtca ggctgtggct
     9901 gacttttttg gtggtttgat tgaggcaggc gtgattgagc atgccctcac catgaaagag
     9961 gctgttgtga gcgataccat ggcgacgcaa acaggtaact gggcggctgc aatgattggt
    10021 gaagatggtg tgaagcaaca gtttagtggc aatgtgcaag tcgttttcca gaaacagtca
    10081 gatggccagt ggctggcagt gacgcatatc tggaactaat caatacggaa tttatttttc
    10141 ggaacggacc atatggatca actgatcaaa caacgttcgc aggcgctcac tgccttgctc
    10201 gtactcacca tcatctgggg ttataactgg gtggtgatga agagcgcgct gcaatatgcc
    10261 gggccgttcc agtttgcggc catgcgtacg ttttttggtg cgctggtgct gttcctggtg
    10321 ttgtatgtga cgcgtcgccc aatgggtttg acggagtttc cgaccatgct gctgctgggc
    10381 attttgcaga acgttggctt tactggtgtc ctgatgtggg cgctggtaga aggcggtgcc
    10441 ggtaaaacgg cagtgctgac ctacaccatg ccgttctgga ccctgctgtt tgcctggccg
    10501 atgctgggtg agcacgtgca cggttggcaa tggctggcgg tgattgcggc agtgctgggt
    10561 atgctgttca tctttgatcc gctacatatc cgtgcggata gtttcagtat gttgttggca
    10621 gtattgtccg gcgtgtcatg ggcattgtcc gccatcatga ccaagcagct gcataggcgt
    10681 catccgacgc tggatttgat caacctgacc gcctggccga tgttgctggg ttcattgccc
    10741 atggtgctga tcgcctggct ggtgcctgcg ccgccaatag actggtcacc gtacctgatc
    10801 ggtgcggtat tgtttaacgt ggtgctgtgt ggtgcgttgg cctggctgtt gtggctgttt
    10861 gccttgcaac ggttgccggc cggggtggca agtatggcgt ctatgctggc gccggtgatt
    10921 ggtgtgattg cggcctggat acagttgaac gaagtgccgg atcatatgga gatgacgggg
    10981 atggcattga ttatcttttc attgagcctg atttccttcc tgatgatgcg ccctggttac
    11041 ggcaaaagcc aataagcgaa cgctagtttg aaaaaaaagc ctgacattgt caggcctttt
    11101 ttgttgtgtg cggcggtcgc ttggtgatcg ggcggctgtg gtaaaatcat cgggttaaat
    11161 tacctcaaca acgttgctaa aaaatacaag gaagcactat ggctgggcat agtaaatggg
    11221 ccaatattca acaccgtaaa ggccgtcaag acgccaaacg cggcaagatt ttcaccaagc
    11281 tgatcaagga aatcaccgtt tctgcgcgca tgggcggcgg cgataccgcc atgaatccgc
    11341 gcctgcgtgc ggccgtggct gcggctaagg aagaaaatat gccttcagac aatatcacgc
    11401 gtgcgattaa aaaaggcacg ggtgagctgg aaggcgtgaa ttatgaagaa attcgttatg
    11461 aaggctatgg tatcaacggt gcggccatta ttattgattg cctgactgat aaccgtcagc
    11521 gcgcggtaat ggatgtgcgt cacgccctga ccaagcacgg cggcaacctg ggtaccgatg
    11581 gtagcgtggc cttcatgttc aagcactgtg ggcaattggt gtttgagcca ggcgtgtcag
    11641 aagatgcggt gatggaagtg gcgttggagg cgggcgcgga agatgtcatt accgacgaag
    11701 atggcagtat tgaagtgatt actgccccgg gcgacttcgt gaccgtcaaa gaggcgctgg
    11761 aagccgctgg cctgaaagcc gtgatggcac aagtgacgat gaaatcactg aatgaagtgg
    11821 tattcaccgg tgacgacgcg gtcaaaatgc agaaaatcct ggatatgctg gaagatctgg
    11881 atgatgtgca ggatgtgtac acttcagccg tgattgaaga gtaatccgtc atcattgact
    11941 ggatgattcg ttttcaatgg ctgcgcaacg tatcttaggc atagatcctg gcctccgaat
    12001 aaccggtttc ggtgtgatcg aggccgtgga tggcaagttg tcctatatta ccagtggtgt
    12061 cattaaaaca gcttctgcca aaaaaactgc aaagggggag tctgaaaaag aagccttgcc
    12121 tgagcgattg aaagtcattt tagatggctt gtttgaggtt attgccacct atcagccgac
    12181 gcaagtcgcg atagaaaaag tgtttgtgaa cgtgaacccg caatcgactt tgctgttagg
    12241 ccaggccaga ggggctgcga tttctgcagc tgtcatccgc cagttatctg tcgccgaata
    12301 tactgctttg caagtcaagc aatccgtggt cggcaatggc catgcgcaaa aagagcaggt
    12361 gcaggaaatg gtgaaacggt tattaaaatt acctgctaat ccaagcccgg actcggcgga
    12421 tgcgctggca tgcgccatct gtcatgcaca tgggggacaa gggctggggg cactggcaac
    12481 cacaggattc agggtgagga atggaagatt aatatgattg cgcgtttgca gggtaagtta
    12541 ctggaaaaat cacccccctt gatcgtggta gattgcaacg gtgttggtga tgaagtggaa
    12601 gtacccatga gcacttttta taacctgcca gaattgggtc aatccgtgca gatattgacg
    12661 catatggtgg tgcgcgaaga tgcgcagttg ctatatggtt ttggcagtga gcaggaaaaa
    12721 aataccttca agcaactgtt aaaagtcaac ggtatagggg cgaagtctgc gctgtcgatc
    12781 ctcagtggcg tcagcgtcaa tgatttgatg ctggcggtga gccagcaaga ggtttctatg
    12841 ttgacccgta ttcctgggat tggcaaaaaa accgctgaac gtttattgct ggagctcaag
    12901 gacaagtttg ttgttaccgg atcgacctca gcgcagcctg ctgccaaagc ggcaacggat
    12961 gatatcctca atgcattggt ggcgctgggc tatcacgagc gcgaggctgg ggcgactgtc
    13021 aagttgctgg aacctggcgt cagcgtcagt gatggtatcc gccaggcgct caaatatctt
    13081 gccaagtaac agtgtgacgg cagacctcgg tctgtaatag tcaaacgctg catggtatga
    13141 tggtgaaaaa ataacttcat caacagcggc atgaggccct atattgttta gtgttgtact
    13201 gcacttcatc gccgatcact catgatagaa accgatagac ttgtagctcc tgccactgcc
    13261 agcgctcagg aagaagcgtt ggaacgtgca ttgcgcccca aactgctgga cgaatatgtc
    13321 gggcaggaaa aagcccgtgg ccagctcgag attttcatta atgccgcccg tggccgccag
    13381 gaagcgttag accatgtgct gctatttggc ccacctggtc tcggtaaaac cacccttgcc
    13441 catattgttg cgaaagaaat gggtgtcaat ctgcgtatga cctctggtcc ggtattggag
    13501 cgcgctggtg atttggccgc gttgctgacc aatcttgaac ccaacgatgt cctgtttatt
    13561 gatgaaattc accggttaag tcctgtggta gaagaaattc tttatccggc aatggaagat
    13621 taccgtttgg atatcatgat tggtgaaggt ccggctgcac gcagtgtgcg tctagacttg
    13681 ccgcccttca cgctggtggg agccactacg cgtgcgggca tgttgaccaa tcccctacga
    13741 gaccggtttg ggattgtggc acgtctggaa ttttatagtc atgaagagct ggccaagatt
    13801 gtcagccgtt cagccaattt gttagaggtg gatatgcagt ctgctggcgc gctcgaaatc
    13861 gcgcgccgcg cacgtggcac gccacggatt gccaaccgtt tgttacggcg ggtgcgcgat
    13921 tatgctcagg taaaatctga tggtatcgtc acagatgtga ttgcagatgc cgctttgaaa
    13981 atgctggatg tggatcagca agggctggat gtcatggacc gcaagctgtt gcaggccgtg
    14041 gttgaaaaat ttggtggtgg tccggttggc ctggataatt tggcggcggc cattggtgaa
    14101 gagcgcgata cgattgaaga tgtattggag ccttatctta tccagcaagg atatctgatg
    14161 cgaacaccac gcggacgcgt ggcgaccatg ctgacttatc agcattttgg cttgaagccg
    14221 cctgcaagca tggtgtcagg tgatttgtgg caagatggtc aataatatgt ttgtatttcc
    14281 agtccgcgtt tattacgaag ataccgatgc cggtggcgtg gtttatcata gccgttacct
    14341 gaattttatg gagcgggcac ggactgaatg gttgcgggct gctggttttg tgcagactgt
    14401 tttaaagcag caggaacagg tgttgtttgt ggtgcatagc atgcagctgc agtttaaaaa
    14461 gccagcccgt ctcgatgagg agttgaaggt ggtttcacaa ctgattgaaa tggggcgcgg
    14521 cagctttagt tgcaggcaac aaatcatacg cgatgaaacc gttttaattg aggcacaggt
    14581 caaggtggcc tgtgtggatg cagattcatt taaacccacg ggtattccgg cccgtatcaa
    14641 aattgcactg gagtcttcat gaatgtaacg aacgatatgt cgatttttac cctggtgagc
    14701 ggggccagtt tgccagtaca actggtgatg ctgatcttgc tgctgacctc gctggtctcc
    14761 tggtggtata tcttcattaa agtgttttca ctgaaggctg ccaatgaaaa tgtggaccag
    14821 tttgaagcac agttctggtc tggtggtgac ctgaacgaat tgtatgaacg cattaccgca
    14881 ggcagaaaaa caccccaggg catggccagc attttccagg ccgggcttaa ggagtttatc
    14941 cgctacaaac agcaaaaaga tacagaagtt agtgacgtga tggaagcgac acgccgcgcc
    15001 atgcgtgcgg catataaccg tgaactggat tacctggatt cgcatttacc attcctggct
    15061 tccgtcggct cggtcagtcc ttacatcggc ttgttcggta cagtatgggg cattatgaac
    15121 gctttccgcg gcttgtctaa tgtggcacaa gccacattga gccaggtcgc gccaggtatt
    15181 gctgaagctt tggtggcaac cgccattggc ctgtttgcgg cgattccggc cgtgattgcc
    15241 tacaaccgct ttgcggcagg ggttgaccgc ctctctgtgc gctacgagag ctttatcgaa
    15301 gaatttacca acattttgca acgtagagcc tgatcggagt cactatgagc agaacgcgta
    15361 aacggcgcat gatgaaccag attaacgtcg taccttatat cgacgtcacg ctggtattgc
    15421 ttgtaatttt tatggtgacg gcaccaatga cgaaccctgg aatggttgag ttgcctaaag
    15481 tggggcaggc gctcaagcaa agtggtgacc ctatcgtcgt gacggtgcaa aaagaaggta
    15541 ccgtgatgat ggacggcaag gaaatgcagc gtgacgatat gttggcatta attcgtcagc
    15601 gcttatccga agccgaaaag ccagtagtcg tatccgcgga taaaaacacg ccttatggcg
    15661 aagtggtatc ggtcatggat ttacttaagc aggcgcaggt agataaagtc ggcttgctgt
    15721 tccagaacga gaaataagtg gttacaaacc taagcagcca actgacaata gtttaagcga
    15781 cataattcag atgaaaagca cgttgtcccc acaagatcaa gccatacgta agcgtgccgc
    15841 agtgctttcg gtattgatgc atgtgctgtt gtttctgggc atgattttca tgatcgattg
    15901 gaaagcggta catgtgaatc ctagtgtcat ggaagcgacc ttgtgggaca gtttgcctag
    15961 taaacaacct gtcaaaccgg tcacgaaacc tgtggatcca acaccgcctc caccggcgcc
    16021 tgaaccgaaa ccagagccta aaccgcctga accaaagcca ccgcaaccgg agccaaaacc
    16081 agaccctcag gtcaagcaag cggaacaaga agcgcaaatt gcactcaaaa agcaggaaga
    16141 tgaaaaaaaa cggcaattag aggaagagaa aaaaaaggaa gaagagaaaa agaaacagga
    16201 agaaaagctg aagaaaattc aagaagctct gcgtaagcaa gagg
//
LOCUS       DATMSK010000200         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_62793,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000200 DATMSK010000000
VERSION     DATMSK010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62793"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VNZ84_06645"
     CDS             complement(<1..249)
                     /locus_tag="VNZ84_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWU66673.1"
                     /translation="MMRWQVLAILGFSWPWMAAQAEEIADIRVRQRLEKTEGRALETY
                     SRQPGVPLHDAIKQQQNRKPAVTVDLKSALKPFDSPESD"
     gene            complement(246..1553)
                     /locus_tag="VNZ84_06650"
     CDS             complement(246..1553)
                     /locus_tag="VNZ84_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWU66674.1"
                     /translation="MHDQLQMTLMHSVQTPHRLHAWIKRLAWLLVGLPIFFMFLPWQQ
                     NVTALGKVTAYSPSERVQTIDAPVNGLIYRWHVQEGTQVKEGDLLLEISDTDPNFKQR
                     LEAQRDNLQSKLTAKQDELRAYELQKQNLTAARDAKISAAQYKLDVANQKIRSSQESV
                     NAAQATLDAASQQVQRLQRLFGDGLVSKRDLEVAERDHIIASRNLSSAQAQWQSAKAE
                     ASSAAADIQQIRSDTQAYLDSTTAVINKIRGETADSENNLHTSEISLARQNMQRIVAP
                     RDGTIFRLPVNSQSQIIKQGDPLLVIVPHTSQRAVELWVDGRDAPLITSRSTVRLEFE
                     GWPAIQVPGWAQVGVGTFEGEISFVDPTDNGSGHFRIMVVHKPGTPEWPSARFLRQGI
                     SAKGWVLLERVTIGYELWRLLNGFPPRIPPEPVPVHQTGGKPA"
     gene            complement(1546..>2674)
                     /locus_tag="VNZ84_06655"
     CDS             complement(1546..>2674)
                     /locus_tag="VNZ84_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU66675.1"
                     /translation="GKQAEITAIKESKAKYDMAAWLENIARNKWLSKFYGAKQRATAN
                     TELLAQNYLGARVKHFRVLMMQLIGAVGMYALIGTGMLILGGTLVIKGQINLGQFVAA
                     ELIIFGVLSAFVRFITKLEYFYDLLAAVDKLGVLETLPVETSGTHQPEVDGYAPLKVT
                     DLSFSHPDQPPLVRHVSFELQQGQSLAVFGELGSGKSTLLELITGLRQPDHGYVNYHD
                     IDLRQLNLDQLRDQIGIANKVEWQHGSIFENLRLDRAEITLDAMTEILQVLGLWEDIS
                     KLPKGIDTVLTDYGAPMTYTQLQRLMLARAIIGRPHLLIIDGLLDSLGQHELDQVLRL
                     LKRQQAQWMLIVTTRFEHIAQQFQQVIALGSHEGQSGGQHA"
BASE COUNT          615 a          736 c          657 g          666 t
ORIGIN      
        1 gtcactttct gggctatcaa acggtttcaa ggcacttttc aaatccaccg tgacggctgg
       61 tttgcggttt tgctgttgct taatcgcatc atgcaaaggc acccctggtt gccggctata
      121 ggtctccagt gcccgccctt cggttttttc caggcgctgc cgtacacgta tgtctgcaat
      181 ctcttcagct tgcgcggcca tccaaggcca agagaatccc agtatggcca agacttgcca
      241 ccgcatcatg ctggtttgcc tccggtctgg tgcacaggca caggctctgg cggaatacgc
      301 ggcggaaaac cgttgagcaa acgccacaat tcatagccaa tggtcacacg ctccagcaac
      361 acccagccct ttgcactaat gccctggcgc aaaaagcgcg cagatggcca ctccggagtc
      421 cccggcttgt gcacgaccat gatacggaaa tgaccactac cattatctgt tgggtcaaca
      481 aatgaaattt caccttcaaa ggtgccgaca cccacctggg cccagccagg cacctggatc
      541 gcaggccagc cttcaaactc aagcctgacc gtgctgcggg aagtgattaa gggtgcatca
      601 cggccatcca cccagagctc aacggcacgc tgggaggtat gcggaacaat gactagcaaa
      661 ggatcgcctt gcttgatgat ttgtgattgc gaattgactg gcaacctgaa aatggtgcca
      721 tctcttggcg ccacgatgcg ttgcatattc tggcgtgcca gcgaaatttc ggaggtatgc
      781 aggttattct cactgtccgc agtttctccg cggattttat tgataacggc tgtggtggaa
      841 tccaggtagg cctgggtatc agagcggatt tgctggatat ctgccgcggc agagctcgcc
      901 tccgctttgg cagattgcca ttgtgcctgc gcactactca ggttgcggct ggcaataata
      961 tggtcacgct cagccacttc aaggtcacgt ttagatacca agccatcccc aaacaggcgc
     1021 tgaaggcgtt gcacttgctg gctggcggca tccagggtcg cctgcgccgc attgacggat
     1081 tcctggctgc tacggatttt ttggttggcg acatccagct tgtattgggc ggccgaaatt
     1141 ttggcatcac gcgctgcggt caggttctgt ttttgcagct cataagcccg taactcgtcc
     1201 tgcttggcag tcagtttact ttgcaggtta tcgcgctgtg cttccaaacg ctgcttgaaa
     1261 ttggggtcag tatcgctgat ctccagcaag agatcacctt ctttgacttg cgtgccctct
     1321 tggacatgcc agcggtaaat gagcccattc accggcgcgt ctatcgtttg tacacgctca
     1381 gaaggggaat acgcggtgac tttgcctagt gcagtcacgt tttgctgcca tggtaaaaac
     1441 atgaagaaaa ttggcagccc aaccagtagc caagccaggc gtttaatcca tgcatgcaag
     1501 cgatgtggtg tttgcacact atgcattagg gtcatctgca actgatcatg catgttgacc
     1561 tcctgactgc ccctcgtgac tgcccagggc aatcacttgc tggaactgtt gcgcaatatg
     1621 ctcgaatcga gtcgtgacga tcagcatcca ctgtgcttgc tgacgcttga gtaatctcag
     1681 cacctgatcc aactcatgct ggcctagact gtctagcaaa ccatcgataa tcagcaagtg
     1741 cggacggcca atgatggccc tggccaacat cagccgctgc aactgtgtat aggtcattgg
     1801 tgcaccataa tcggttaata cggtgtcgat tcctttgggt agcttgctaa tatcctccca
     1861 caagcccagc acctgtaaaa tctcggtcat tgcatccaaa gtgatttctg ccctatccag
     1921 gcgtaaattc tcaaagatag acccgtgttg ccactcaacc ttattcgcaa tcccaatttg
     1981 gtcacgtaat tgatccaggt tcagttggcg taaatcaatg tcatgatagt taacataacc
     2041 gtgatcgggc tgcctgaggc cggtaataag ctccagcagg gtacttttgc cactgcccaa
     2101 ttcaccaaag acagccaggc tctggccttg ttgtagctca aaactgacat gcctcactag
     2161 cggcggttgg tcaggatggc taaaactgag atccgtcact tttaatggcg cataaccatc
     2221 aacttcaggc tgatgtgtcc cggaagtttc taccggtaac gtctccagta cgcccagctt
     2281 gtccacggca gccagcaaat cgtaaaaata ttcgagcttg gtgatgaagc gcacaaacgc
     2341 tgataacaca ccaaaaataa tcagctcagc cgctacgaac tggcccaaat tgatctggcc
     2401 cttgatcacc aaggtgcccc cgagaatcag catgccggtg ccaatcaagg catacatacc
     2461 gacagcaccg atcagttgca tcatcaatac acgaaaatgt ttgaccctgg cacccagata
     2521 gttttgtgca agcagctctg tattggctgt cgcccgttgt ttggcaccat aaaacttcga
     2581 tagccatttg ttacgtgcaa tattctctag ccaggcggcc atgtcatact tggcttttga
     2641 ttctttgatt gcagtgattt ccgcctgttt acca
//
LOCUS       DATMSK010000201         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_203420,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000201 DATMSK010000000
VERSION     DATMSK010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203420"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1552)
                     /gene="guaB"
                     /locus_tag="VNZ84_06660"
     CDS             complement(92..1552)
                     /gene="guaB"
                     /locus_tag="VNZ84_06660"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWU66676.1"
                     /translation="MRLLQQALTFDDVLLVPAHSNVMPREVNLSTKLTKSITLNIPLL
                     SAAMDTVTEARMAIALAQLGGMGIIHKNMTVEMQASRVSRVKRFESGVVIDPVTIHPA
                     MSVREVMDITKQQKISGLPVLQDGKVVGIVTNRDLRFETNLDQAVSNVMTPQDRLVTV
                     REGASREEIMGLLHKHRLERLLVVGENYELKGLITVKDIQKSSDHPLACKDAKGRLRV
                     GAAVGVGDGTDERIAALVEAGVDAIVVDTAHGHSQGVLDRVKWAKTRYPDLQVIGGNI
                     ATAEAALALVDHGADCVKVGIGPGSICTTRIVAGVGVPQITAISNVAKALEKSGVPFI
                     ADGGIRFSGDISKAIAAGAYNVMLGGMFAGTEEAPGEVELYQGRSYKSYRGMGSIGAM
                     QKGSSDRYFQDNTANADKLVPEGIEGRVPYKGSVVAVIHQLMGGLRASMGYVGCSTIE
                     EMRNKAEFVQITSAGMRESHVHDVQITKEAPNYRID"
     gene            1695..2174
                     /locus_tag="VNZ84_06665"
     CDS             1695..2174
                     /locus_tag="VNZ84_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HWU66677.1"
                     /translation="MKMTLKLMLVWVLCCSLPAGAEIYKWRDEKGIMNYSDVPPKNTG
                     TGTQKIKAATVPNDIPLTRSDAYQHEGDSASANKKPGESREAANQSPEAEALKLMQER
                     VKAQNCAAARSNYRNYSVGGRMQNVNENGQKEYLSDDQIREGLARAQQEIDEYCPPE"
     gene            complement(2194..2511)
                     /locus_tag="VNZ84_06670"
     CDS             complement(2194..2511)
                     /locus_tag="VNZ84_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HWU66678.1"
                     /translation="MRQAPTDTIPVEVAYALPHKQSLIALDVLTGTSALQAVMQSRIL
                     EKYPELNASKLTLGIFGKRVAADTILHAFNRVEIYRPLLADPKEARRQRAAAKNGKLK
                     QNK"
     gene            complement(2516..>2847)
                     /locus_tag="VNZ84_06675"
     CDS             complement(2516..>2847)
                     /locus_tag="VNZ84_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HWU66679.1"
                     /translation="GSELIERTEEITQATIHIRYHGIQQHFTTRNHKQFPNRMEITLV
                     DGPFKQLAGHWHFTALREDACKIEFKLEYVFANSLVERIIAPVFSHIANTFVDSFVKQ
                     ADKLRLK"
BASE COUNT          690 a          686 c          748 g          723 t
ORIGIN      
        1 atcaggattt ttgagtgcat aaattttcag gtcactagaa tagacaaaag cgcagcggat
       61 aaactgctgc gcttttacgt ttaaacaaat attaatcaat cctgtagttg ggggcttctt
      121 tcgtgatttg cacatcatgc acatgcgact cgcgcatgcc ggcactggtg atctgtacaa
      181 attcggcctt gttgcgcatt tcttcgatag tggagcagcc aacatagccc atagaggcgc
      241 gcagaccacc cattaactgg tgaatgacgg cgacgacact gcctttgtag ggtactcggc
      301 cttcaatgcc ttcggggacc agtttatcag cattagctgt attgtcctgg aagtagcggt
      361 cagaagagcc tttttgcatg gcgccaattg agcccatgcc gcgataggac ttgtatgagc
      421 ggccttggta taactccact tcacctggcg cttcttcagt cccggcaaac ataccaccca
      481 gcatgacgtt ataggcgcca gctgcaatcg cttttgaaat gtcgccagag aaacggatac
      541 caccgtcggc aataaagggc acgccggatt tttccaacgc tttggccaca ttgctgatgg
      601 ccgtgatttg cggcacacca accccggcga cgatacgggt ggtacagata gagcctgggc
      661 caataccgac tttgacgcag tcggcaccat gatccaccaa ggctaacgcg gcttcagcgg
      721 tggcaatgtt gccaccaatc acttgcaggt ctgggtaacg tgttttagcc catttaacgc
      781 ggtccagcac gccttgggag tggccatgtg cagtgtcgac cacaatcgca tccacacctg
      841 cttctaccag ggccgcaatg cgttcgtcgg taccatcacc cacgcccaca gcagcgccta
      901 cgcgtaagcg gcccttggca tctttgcatg ccaatgggtg gtcggatgat ttttggatgt
      961 ctttaacagt aatcaggcct ttgagctcgt agttttcacc gacgaccagc aaacgctcca
     1021 ggcggtgttt atgcaacagg cccatgattt cttcacggct ggcaccctca cgcacggtaa
     1081 ccaagcgatc ttgcggcgtc atgacgttac tcacggcttg atccaggttg gtttcaaagc
     1141 gcaggtcgcg attggtgaca atgccaacca ctttgccatc ctgcaacaca ggcaggccag
     1201 aaatcttttg ctgtttggtg atgtccatca cttcacgcac gctcatggca gggtgaatgg
     1261 ttactgggtc aatcacaacg cctgattcga aacgttttac gcgggagacg cgggaggcct
     1321 gcatctcaac cgtcatgttt ttatgaataa tccccatgcc gcccaattgt gccagggcaa
     1381 tggccatgcg tgcttcagtg acagtgtcca tcgcagcaga taacagtggg atatttaaag
     1441 tgatgctttt ggtcagcttg gtactgaggt tgacctcgcg aggcatgaca ttcgaatggg
     1501 ctggaacgag taaaacgtca tcaaaggtca gggcttgttg caacaaacgc atgtgaattc
     1561 cttatctcca aaatataatt ataccgattt cgttttgcct tgacaaagcg ttttgcattt
     1621 tttgccgaag atatggagca gtttggcatg gtttatgcat ggatgttttc gaataaagta
     1681 tgcgagtgag attgatgaag atgactttaa agttgatgtt ggtttgggtg ttgtgctgca
     1741 gcctgccagc aggcgctgaa atttataagt ggcgcgatga aaaaggcatc atgaattatt
     1801 cggatgtgcc accaaaaaac acagggacag gcacgcaaaa aatcaaggcg gccaccgtgc
     1861 cgaatgatat tccactgaca cgctcggatg cctatcagca tgagggcgat tcagcttctg
     1921 ccaacaaaaa acctggcgaa agtcgggagg ctgccaatca gtcgcctgaa gctgaggctt
     1981 taaagctgat gcaagagcgt gtgaaagcgc aaaactgtgc tgccgcacgt agtaattacc
     2041 gcaattattc agttggtggc cgcatgcaaa atgttaatga aaacgggcag aaagagtatt
     2101 tgagtgacga ccagatcagg gaaggtttgg ctcgtgcaca gcaagagatt gacgaatatt
     2161 gtccacctga gtaagcaaat aacgtcaacg acgtcattta ttttgtttta acttcccatt
     2221 tttggcagct gcgcgctgtc gtctcgcctc tttaggatcg gcaagcaggg ggcggtaaat
     2281 ctcgacccga ttaaaagcat gcaagatggt gtcggctgcg acccgtttgc caaagatgcc
     2341 taatgtgagt ttgctggcgt tcagctcggg atatttctcc aggattctgg attgcatgac
     2401 agcttgtaat gcagaggtcc cggttaagac atcgagtgcg atcaagcttt gcttgtgtgg
     2461 caaggcataa gctacttcaa ccgggattgt gtcagtgggt gcttggcgca tcaatttact
     2521 tcaagcgcag cttgtctgcc tgtttgacaa agctgtctac aaaggtgttg gcgatatgac
     2581 tgaataccgg cgcaatgatg cgctccacca ggctgttggc aaacacatac tccagtttga
     2641 attcgatttt gcaggcatct tcacgtaaag cggtaaagtg ccagtgaccg gccagttgct
     2701 tgaaagggcc atctaccagc gtaatctcca tgcggttagg aaactgtttg tgattgcgtg
     2761 tcgtgaagtg ctgttgaatg ccgtgatagc ggatatgtat tgtggcctgg gtgatctctt
     2821 ccgtccgctc aatcagttcg ctgccac
//
LOCUS       DATMSK010000202         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_344589,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000202 DATMSK010000000
VERSION     DATMSK010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344589"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VNZ84_06680"
     CDS             <1..225
                     /locus_tag="VNZ84_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HWU66680.1"
                     /translation="LFQTMPQLSHEWRLFIAMGFLGGLTTFSTFSAQAFILLQKQAYA
                     WAGLHVLTHVIVSVVLTILGYWCVMRWPST"
     gene            complement(299..2962)
                     /locus_tag="VNZ84_06685"
     CDS             complement(299..2962)
                     /locus_tag="VNZ84_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWU66681.1"
                     /translation="MKRMLFNATQSEELRVAIVDGQKLIDLDIEHAGKEQRKSNIYKG
                     VITRIEPSLEAAFVDYGTDRHGFLPFKEIARSYFQDKPEQGRARIQDVIKEGQEVIVQ
                     VDKDERGSKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEERNELRDTMAQLEVP
                     NGMSIIARTAGIGRSAEELQWDLNYLSQLWQAIEDASNFQPGAYLIYQESSLVIRAIR
                     DYFSADIGEILIDTVDVHEQALQFMNHVMPGNVTRVKLYQDEIPLFSRFQIEHQIESA
                     FAREVRLPSGGAIVIDHTEALVSIDVNSGRSIKGADIEQTAFNTNLEAAEEVARQMRL
                     RDLGGLVVIDFIDMENQRNQRDVENALRDALHHDRARVQMGKISRFGLLELSRQRLRP
                     SLGETNHMTCPRCNGTGHIRGIESTALHILRITQEEAMKDNSAIIQVQLPVEAATFLL
                     NEKRADIHKIEQRTGVEVILIPNIHMETPNYNILRIRHDDVNEETTQASYKMVDLPSE
                     SDYQHRLQQAAAPAKTEAVVKGITPATAAPIVEEKPAAAPATEAKKSFFGVIKSWLGL
                     EEKEVVQEVEKPARESRESGRDRNKNRNRRDRQRERNQERGERPAKEENTHNKQRQER
                     QAQEKPVQKDRQERQAAQDTAKTDVNQPALTTQASENKQERGERGNRRNRHGRRDRRQ
                     RDESSPATTATANVTVETATVDAAPVVTTEMTPATQEAANVKPAAQTVDPVAQAPVTE
                     SVAEKNESAKAETSTAPSEESSTEKKAPRSRSRTSKSSKTRGKQAADKTEAPAIELVE
                     TTSAPTSVEATSETETPKKKPARKRKPAAKTAEPVADAGIQLVETKSEQAVSAEAPAK
                     VKKPRTAAKWKKEEANAAADAGLVMVETQKD"
     gene            3558..>4442
                     /locus_tag="VNZ84_06690"
     CDS             3558..>4442
                     /locus_tag="VNZ84_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWU66682.1"
                     /translation="MMSQIRPSALQPATTASDGRPASDRVTHLLVDEASETQRIDNYL
                     AKVLKGVPKSHIYRILRSGEVRVNKKRVDAEYRLQLGDEVRVPPIRIAEAASKPTVSS
                     VKAKLAEQVIYEDDAMLALNKPAGMAVHGGSGISLGVIEQLRLERPQAKFLELVHRLD
                     RETSGVLMIAKKRSALTALHDAMRHHQMDKRYLMLVQGQWQDSKKKVTLDLQKYVTES
                     GERRVQVVDEPLLNIEPGGRKSLKASGLVAKRHAAKGRFEVETQISETWFYLQEKLGA
                     YSFLQAKLITGRTHQLRVQ"
BASE COUNT          979 a         1089 c         1154 g         1220 t
ORIGIN      
        1 ttatttcaaa ccatgccaca actgtcacat gaatggcgct tgtttatcgc gatgggcttt
       61 ctgggcgggc tgacgacttt ttccactttt tcggcacaag cattcatctt attgcaaaag
      121 caggcttatg cttgggctgg cctacatgta ctgacacatg tcatcgtttc agtggtactc
      181 accatcctgg gatattggtg tgtgatgcgt tggccttcaa cataagcggt tgattggaca
      241 aaaaaagagg accctgcggt cctctttttt atttgcgatg ttttgatgac accagaattt
      301 aatctttctg cgtttctacc atcaccaatc cagcgtctgc tgcagcattg gcttcctctt
      361 ttttccattt ggccgcagta cgtggttttt ttaccttggc gggtgcttca gcgctaacag
      421 cctgttctga tttggtttct accaattgaa tccctgcgtc ggcaacgggt tctgctgttt
      481 tcgcggcagg tttacgtttg cgtgcgggtt tctttttggg tgtttcagtc tctgacgttg
      541 cttccactga agttggtgca gaggtggttt ccaccaattc tattgcaggt gcttcagttt
      601 tatctgctgc ctgcttgccg cgcgttttac tagatttgct ggtgcgtgag cggctacgtg
      661 gtgctttttt ctcagttgag ctctcttcgc ttggtgctgt acttgtttca gccttggcgc
      721 tttcattttt ctctgcaaca gactctgtca caggcgcttg tgcaactggg tcaacggttt
      781 gagccgctgg cttcacgtta gctgcttctt gggttgcagg tgtcatttct gtcgtgacaa
      841 ctggcgctgc atcaactgtt gcagtttcaa cagtaacatt agcggttgcg gttgttgccg
      901 ggctggactc atcacgctgg cggcgatcac ggcgtccgtg acgattacgg cggttaccac
      961 gttcaccacg ctcctgtttg ttctcgctag cttgcgtcgt cagtgctggc tggtttacat
     1021 ctgttttagc tgtgtcttgc gcggcttgac gctcttgtct gtctttctgc acaggttttt
     1081 cttgtgcctg gcgttcctga cgctgtttgt tatgagtgtt ttcctcttta gccggacgct
     1141 cgccacgctc ctggttacgc tcacgctggc gatcacggcg gttgcgattc ttattgcgat
     1201 cacgaccaga ttctctgctt tcgcgggctg gtttttcaac ctcttgcact acttcttttt
     1261 cttcaaggcc cagccaggat ttgatcacac cgaagaacga ttttttggct tcggttgcag
     1321 gtgctgcagc tggtttttct tctacgatag gtgcagcggt tgcaggggtg atgcccttaa
     1381 ctaccgcttc agttttggct ggcgcggcag cttgctgcaa gcggtgctgg taatcagatt
     1441 cgctaggcaa gtccaccatt ttgtagctgg cctgcgtcgt ttcttcattc acatcatcat
     1501 gacggatacg caggatgttg taatttggcg tttccatatg gatgttagga atcaggataa
     1561 cctctacgcc agtacgctgt tcgattttgt ggatatctgc acgtttttcg ttgagcagga
     1621 aggttgcggc ttcaacgggc aactgcacct ggataatcgc gctgttgtct ttcatcgctt
     1681 cttcttgcgt aatacgcagg atatgcaacg cagtcgactc aatgccacgg atatggccag
     1741 tgccattaca acgcgggcac gtcatgtgat tggtttcacc caggctaggg cgcaagcgct
     1801 gacgtgaaag ctccagcagg ccaaatcgag aaattttgcc catttgaacg cgggcgcggt
     1861 cgtgatgcag ggcatctcgc aaggcgtttt ccacgtcacg ctggttgcgc tggttttcca
     1921 tgtcgataaa gtcgatcacg accaggccac ccaagtcgcg caggcgcatc tggcgcgcca
     1981 cttcttctgc cgcttccagg ttggtattga acgcagtttg ttcaatatcg gcacctttga
     2041 tggagcggcc ggagttgacg tcgatagaga ccagtgcctc agtgtggtca attacaatcg
     2101 cgccaccaga aggcaggcgc acttcacgtg caaacgcaga ttcaatctgg tgttcgatct
     2161 ggaagcgtga gaaaagcggg atttcatcct gatacagttt gacccgagtg acattgccag
     2221 gcatcacgtg gttcatgaac tgcaaggctt gttcgtgcac gtcaaccgta tcaatcagga
     2281 tttcgccaat gtcggcgctg aagtaatcac ggatggcacg gatcaccagg ctgctttcct
     2341 gataaatcag gtaagcacca ggctggaaat tggaagcatc ttcaatggct tgccacagtt
     2401 gggataaata attcagatcc cattgcagtt cttctgcgct acgaccaata ccggccgtgc
     2461 gggcaataat gctcatgcca ttaggcactt caagctgtgc catggtgtca cgcaactcat
     2521 tgcgctcttc gccttccacc cggcgtgaaa caccgccgcc gcgaggattg tttggcatca
     2581 ggaccagata gcggccagcc agggagataa aagtggtcag cgcggcacct ttgctgccac
     2641 gttcgtcctt gtcgacctgg acaatgactt cctgaccttc cttaatcaca tcctgaatac
     2701 gggcgcggcc ttgttcaggc ttgtcctgga agtagctgcg ggcaatttcc ttaaaaggca
     2761 aaaagccgtg tctgtcagta ccatagtcga caaacgcggc ttcaagtgaa ggttctatgc
     2821 gtgtaattac acctttgtaa atattgcttt tacgctgttc tttaccagcg tgttcaatat
     2881 ccaagtcgat gagtttttgt ccatcgacaa tcgcaacgcg cagttcttct gattgcgttg
     2941 cgttaaacaa cattcttttc atgtagggct cctaagtcga agccacagaa tagccatcag
     3001 tgccaggcgg gccagggtta taaatcaacc accagaattt aacgaagtga cctgattgtc
     3061 acacagaaat gtgcaatggc ttttgcgagg ctgactaaac agcatggcag gcatctatat
     3121 gcactgagta cagcagcgct ttagctgcgg aatactcgtt ttctgttagg gtgcagtgag
     3181 tgcctggcag ggttgtgtga cctgtaatat ccatcgacat tgactgcaaa aataaaaccc
     3241 taaacagagg gtaatttcgt taaaattcgg ttttggcaaa cgccgagctc atttgtgtgt
     3301 ttattctgcg tgagcttcgg gttaaatcat ttctaattaa ttatcgctac ttgatggcga
     3361 gcggtattca ccaaaatttt ttgattctga atacacttgc gagcctgtaa atctgggccc
     3421 ggaatgtcag gggttggtat cgatggtaat ctcgagttcg gcactgcgat gttgtcgcca
     3481 tggccgggaa gaccgctgat tcagaatctc gacagtatat ctataagtcc tgataaagca
     3541 aagcacgacc tgcaactatg atgtcacaaa ttcgaccatc ggcattacag ccagcaacca
     3601 cggcctctga cgggcgtcca gccagtgacc gcgtcacgca tttactggtg gatgaggcct
     3661 cagaaaccca gcgcattgat aattacctgg ccaaagtact caaaggcgta cccaaaagcc
     3721 atatttatcg tattttgcgt agtggcgaag tacgtgtgaa caagaaacgt gtagatgccg
     3781 aataccgttt gcaactcggt gacgaggtca gagtaccgcc tatccgcata gcggaagctg
     3841 cgtcaaaacc aacagtaagt agtgtcaaag ccaaactggc tgagcaagtg atttatgaag
     3901 atgacgccat gctggccttg aacaagccag ctggtatggc tgtgcatggt ggcagtggca
     3961 tcagcctcgg ggtgattgaa cagttacggc tggagcggcc acaagccaaa ttcctggagc
     4021 tggtgcaccg gctggaccgt gaaacatcag gcgtactgat gattgccaaa aaacgctcgg
     4081 cactgacggc attgcatgat gccatgcggc atcaccagat ggataaacgg tacctgatgc
     4141 tggtgcaagg acaatggcaa gacagcaaaa agaaagtcac gctggactta cagaagtacg
     4201 tgaccgagag tggggagcgc agggtgcaag tggtcgacga acctttactg aatatcgaac
     4261 caggtggcag gaaatcactc aaagcaagcg gtcttgtagc caagcgccat gcggccaaag
     4321 gcagatttga ggtggaaaca caaatctccg aaacctggtt ttacctccag gaaaaattag
     4381 gcgcctacag ctttctgcaa gccaagctga ttacgggacg tacccatcaa ttacgtgtgc
     4441 aa
//
LOCUS       DATMSK010000203         5017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_109907,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000203 DATMSK010000000
VERSION     DATMSK010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109907"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /gene="guaA"
                     /locus_tag="VNZ84_06695"
     CDS             <1..1368
                     /gene="guaA"
                     /locus_tag="VNZ84_06695"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWU66683.1"
                     /translation="ICYGMQTMAQQLGGKVEARAKREFGYAQVRARGHSALFRDIQDA
                     TNAEGHGLIDVWMSHGDKVTELPVGFKVIASNDTTPIAAMADEDRRFYAVQFHPEVTH
                     TKQGQAMLERFVLDICAAKPDWIMGDYISEAVAKIKAQVGDEEVILGLSGGVDSSVAA
                     ALIHRAIGDQLTCVFVDHGLLRLNEGDMVMEMFAGRLHANVIRVDATEQFMGKLAGVS
                     DPEAKRKIIGAEFVEVFKAEAAKLKASGAGHKGATFLAQGTIYPDVIESGGAKSKKAV
                     TIKSHHNVGGLPEQLGLKLLEPLRDLFKDEVRELGVALGLPRDMVYRHPFPGPGLGVR
                     ILGEVKREYALLLQRADAIFIEELRNTVDATSGKTWYDLTSQAFTVFLPVKSVGVMGD
                     GRTYDYVVALRAVVTSDFMTAHWAELPYSLLGKVSNRIINEVRGINRVVYDISGKPPA
                     TIEWE"
     gene            1414..2433
                     /gene="trpS"
                     /locus_tag="VNZ84_06700"
     CDS             1414..2433
                     /gene="trpS"
                     /locus_tag="VNZ84_06700"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWU66684.1"
                     /translation="MSTKSIILTGDRPTGPLHLGHFVGSLRNRVKYQHEYKQYVMLAD
                     AQALTDNMDDIDKVHRNVVEVALDYLAVGIDPKLSTIFIQSQVPELTELAFYYLNLVT
                     VARLERNPTVKEEIRLRDFERDIPAGFLTYPVSQAADITAFKATLVPVGEDQIPMIEQ
                     TNEIVRRFNRTYKNATTKQEILVETKALVPELGRLPGIDGKAKMSKSLGNVINLGASA
                     DEVTKAVKRVYTDPLHLKIEDPGHVEGNIAFTYLDAFDTDKAAVAELKAHYTRGGLAD
                     SIVKKRLEAVLQEMLEPIRTRRAELADDKGYILQLLREGTEQAREVAAQTMSEVRAAL
                     GLTYF"
     gene            2461..2958
                     /locus_tag="VNZ84_06705"
     CDS             2461..2958
                     /locus_tag="VNZ84_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HWU66685.1"
                     /translation="MSRGFVKEDDLELAGTDLPERPLSPHANYVTPAGLRQLQQAVKT
                     LETDRSQYAARKEDPNAQQKLAEIDRDLRYFAARLETAQLVDPDQQPRHTVLFGAKVT
                     VEDETGEQQVYHIVGEDEADIAKQKVSYVSPVAKALLGRKVGDSTIWKRPAGALSLDI
                     LNIEY"
     gene            2965..3570
                     /locus_tag="VNZ84_06710"
     CDS             2965..3570
                     /locus_tag="VNZ84_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008106925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWU66686.1"
                     /translation="MNVSQAIQERRSIKAYDPAHRMTEAEIEQLLSLAMLSPTAFNIQ
                     HWRFVVVTDPVLRHQIRAVSWNQAQVTDASLLIILTADLKAWEKDPARYWVNAPQPVS
                     DYLVRAIHDYYTDHTQAERDEGMRSGGMAAMTLMLAAKEMGYDTCPMDGFDFDAVGKL
                     INLPADHVPVMFVVVGKALEEAKPRGGQLTMDEVVIYNRFE"
     gene            3629..>5017
                     /locus_tag="VNZ84_06715"
     CDS             3629..>5017
                     /locus_tag="VNZ84_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HWU66687.1"
                     /translation="MLVANNITMQFGSKPLFENVSVKFGDGNRYGLIGANGCGKSTFM
                     KILAGVLEPTSGNVSLDPNERMAFLKQDQFAYEDQRVLDVVMMGHEQMWKAMQERDAI
                     YANLEATEDDYMRAAELEGVFAEYDGYTAEARAGELLLGVGIPIEQHNGLMSAVAPGW
                     KLRVLLVQALFANPDVLLLDEPTNNLDINTIRWLEDVVNNRDCTMVIISHDRHFLNQV
                     CTHTCDMDYGKITSYPGNYDDYMEASIAARNQQAKDNAKAKQQIADLQDFVRRFSANA
                     SKAKQATSRAKQIDKIKVEEFKPSSRQYPFIRFEFDDKEKLHRNAVELKKLSHGFDKP
                     LFNNVEMIFEAGEKVAIIGENGIGKTTFLRCLAGDLQPKHGEVKWAEKAKLGYFAQDH
                     EYEFEKGEDLFEWMTAQRQQGDDDNTVRSMLGRLLFPTEATKKSVKVLSGGEKGRMLY
                     GKLMLAKTNVLLM"
BASE COUNT         1313 a         1265 c         1336 g         1103 t
ORIGIN      
        1 atttgctacg gcatgcaaac catggcgcaa caactgggcg gcaaggtaga agcccgtgcc
       61 aaacgcgagt ttggttacgc tcaagtccgt gcccgcggtc attctgccct gttccgtgat
      121 atccaggatg ccaccaatgc agaaggtcac ggtttaatcg atgtatggat gagccatggc
      181 gacaaggtca ctgagttgcc ggtgggtttt aaagtgattg ccagcaatga caccacaccg
      241 attgcggcga tggccgatga agaccgccgt ttttatgcgg tgcagttcca cccggaagtg
      301 acgcacacca aacaaggcca agccatgctg gagcgctttg tactggatat ttgcgctgct
      361 aaaccagact ggatcatggg cgactacatt agcgaagccg tggcaaaaat caaagctcag
      421 gtcggtgatg aagaagtcat tttaggcctg tcaggtggtg tagactcctc cgtagcagct
      481 gcactgatcc accgcgcgat tggcgaccag ctgacgtgtg tgtttgttga ccacgggcta
      541 ctgcgcctga atgaaggcga catggtgatg gagatgtttg ccggccgttt gcacgccaat
      601 gtgattcgcg tggatgcgac tgaacaattc atgggtaaac tggccggtgt cagcgaccca
      661 gaggccaagc gtaaaattat tggtgctgaa ttcgtggaag tgtttaaagc agaagcggcc
      721 aaactcaaag ccagtggcgc tggccataaa ggcgcgacat tcctggcaca aggcaccatt
      781 tatcctgatg tgattgaatc tggtggtgcc aaatcgaaaa aagccgtgac gatcaagagc
      841 catcataatg tcggcggctt gccagagcag ctaggcttaa aactgcttga accattgcgt
      901 gatttgttca aggacgaagt gcgagaactc ggcgtggccc tgggcctgcc acgtgacatg
      961 gtctatcgtc accctttccc aggaccaggt ttgggcgtgc gtattttggg cgaagtcaaa
     1021 cgtgaatatg ccctgttatt gcaacgtgca gacgcgatct ttattgagga attacgcaat
     1081 acggtagatg ctacctcagg caaaacctgg tatgacctga ccagccaggc gtttaccgtg
     1141 ttcctgccgg tcaaatccgt cggcgtaatg ggagatggcc gtacttatga ttatgtggtg
     1201 gccttacgcg cagtggtcac cagtgacttt atgaccgcac actgggcgga gctgccttac
     1261 agcctattgg gcaaagtgtc caaccgcatt atcaacgaag tccgtggcat aaaccgtgtc
     1321 gtctacgaca tctccggcaa accacccgca accattgagt gggaataact tttaactttt
     1381 gattagcact cacattacac agagaacaaa acgatgagca caaaatccat tatcctgact
     1441 ggcgaccgcc cgaccggccc gttacacttg gggcattttg ttggcagcct gcgtaaccgc
     1501 gtgaaatacc agcatgagta taagcagtac gtcatgctgg ccgatgcaca agccctgact
     1561 gacaacatgg atgacatcga caaagtgcac cgcaacgtgg tcgaagtggc actggattac
     1621 ctcgccgtgg gcattgatcc caagttgtcg accattttca tccagtcaca agtgccagag
     1681 ctgaccgagc tggcatttta ctacctgaat ctggtgactg tggcccgttt ggaaagaaac
     1741 ccgacagtga aagaggaaat tcgcctgcgt gactttgagc gcgatattcc ggctggcttt
     1801 ctcacctatc ctgtcagcca ggcagctgac attaccgcat ttaaagcgac gctggtgccg
     1861 gtgggcgaag accagatccc gatgattgag caaaccaatg aaatcgtgcg ccgttttaac
     1921 cgcacctaca aaaatgcgac gaccaaacag gaaatcctcg ttgagaccaa ggccttggtg
     1981 cctgaattag gtcgcctgcc cggtattgat ggcaaggcca aaatgagtaa gtcgctaggt
     2041 aacgtcatta acctgggcgc cagtgcggac gaagtgacta aggctgtgaa gcgtgtttac
     2101 acggacccac tgcacttgaa aattgaggat ccaggccatg tagaaggcaa cattgccttt
     2161 acctacctgg atgcctttga cactgacaaa gcagccgtcg ccgaattgaa agcccattac
     2221 acacgtggtg gactggcaga cagcattgtc aaaaaacgcc tggaagcggt attacaagaa
     2281 atgctggaac ctatccgcac ccgtcgtgcc gaattggctg acgacaaagg ctacattttg
     2341 caattgctac gtgaaggcac cgagcaggca cgtgaagtgg cggcacaaac gatgagtgaa
     2401 gtccgcgccg ccttgggcct gacctatttc taataccgga gcagactgct aaacgccatc
     2461 atgagccgcg gctttgtcaa agaagatgac ctggaactgg cgggcaccga tttaccggag
     2521 cgcccgctca gcccgcatgc caactacgtc acgccagcgg gcttgcgaca gctgcagcag
     2581 gcggtaaaaa cactggaaac agaccgctca caatatgcag cccgcaagga agatccgaat
     2641 gcacagcaga agctggctga aattgaccgc gacttgcgct attttgccgc ccgcctggaa
     2701 acggcgcaac tggtagaccc tgatcagcaa ccgcgacata cagtgttatt tggtgccaag
     2761 gttaccgtag aggacgagac tggcgagcaa caggtttatc acattgtcgg cgaggatgaa
     2821 gccgacattg ccaagcaaaa agtcagctat gtctccccgg tggctaaagc actgcttggc
     2881 cgaaaagtcg gtgatagtac catctggaaa cggccagccg gtgcattgtc actggatatt
     2941 ttaaacatcg aatattgaaa gattatgaac gtatcacaag ccatacagga acgccgctct
     3001 atcaaagcct atgatcctgc acaccgcatg actgaggccg aaattgagca attactgtcg
     3061 ctggccatgc tctccccgac tgcatttaat atccagcact ggcgttttgt cgtcgtgact
     3121 gacccggtcc tacgccacca gatccgtgcc gtgagctgga accaggcaca ggttactgat
     3181 gcgtctttgc tgatcatttt gacagctgac ctgaaagcct gggaaaaaga cccggcacgt
     3241 tactgggtca acgcgccaca gcctgtgagt gactacctgg tcagggcgat acatgactac
     3301 tataccgacc acacgcaagc tgagcgcgat gaaggcatgc gttcaggtgg tatggctgct
     3361 atgacattga tgttggccgc caaggaaatg ggctatgaca cctgccccat ggatggtttt
     3421 gattttgatg ccgtaggcaa actgattaat ttgccggccg accatgtgcc ggtgatgttc
     3481 gtcgtcgtag gcaaggcact ggaagaagcc aagccacgcg gcggccaatt gacaatggat
     3541 gaagtcgtta tttataaccg ttttgaataa acctgccgtt ttcacaaaaa accgcagcct
     3601 tagttaactt tttatcgaga gttcttgcat gttagttgca aacaatatca ccatgcagtt
     3661 tggctccaag ccactgtttg aaaatgtctc cgtcaaattt ggcgacggta accgctacgg
     3721 cctgattggt gccaacggtt gcggtaaatc cacctttatg aagatcctgg ctggcgtact
     3781 ggaacccacc agcggcaatg tcagtctaga ccctaacgag cgcatggcct tcctgaaaca
     3841 ggatcaattt gcctacgaag accagcgtgt actggatgtg gtgatgatgg gccacgagca
     3901 aatgtggaaa gccatgcagg agcgtgatgc gatttacgcc aacctggaag ccactgaaga
     3961 cgactatatg cgcgcggcag agctggaagg cgtatttgcc gaatatgacg gttataccgc
     4021 tgaagcacgt gcgggcgagt tattgttagg tgtaggcatt ccaattgaac aacataatgg
     4081 cctgatgagc gccgttgcgc ctggctggaa actgcgtgta ctgctggtac aagccctgtt
     4141 tgccaatccg gacgtgttgc tactggatga acccaccaac aacctggata ttaatacgat
     4201 ccgctggctg gaagatgtag tcaacaaccg tgattgcacc atggtgatta tttcgcatga
     4261 ccgtcacttc ctgaaccagg tctgtaccca tacctgtgac atggactacg gcaagatcac
     4321 ctcttaccct ggcaactacg atgattacat ggaagcaagc attgcagcgc gtaatcaaca
     4381 agccaaagat aacgccaaag ccaagcagca aattgccgat ttgcaggact ttgtgcgtcg
     4441 attctctgcc aacgcgtcca aagctaaaca agcgaccagc cgcgccaagc agattgataa
     4501 gatcaaggtt gaagaattca agccttccag ccgccagtat ccctttatcc gttttgagtt
     4561 tgacgataaa gaaaaactgc accgaaatgc agtcgagctg aaaaagctca gccatggctt
     4621 tgataaaccg ttgttcaaca acgtggaaat gatttttgag gctggtgaga aagtcgccat
     4681 cattggtgaa aacggcatcg ggaaaaccac tttcctgcgt tgcctggctg gcgatttgca
     4741 acccaagcat ggtgaagtca aatgggcaga aaaagccaag ctcggttatt ttgcgcaaga
     4801 ccatgaatac gagtttgaaa aaggcgaaga cctgtttgaa tggatgacgg cacaacgtca
     4861 gcaaggcgac gatgacaaca ccgtgcgcag catgttgggc cgcttgctgt ttccgacaga
     4921 agcgactaaa aaatcggtca aagtgctttc cggtggtgaa aaaggccgca tgctgtacgg
     4981 caaactgatg ctggcaaaga ccaatgtgct actcatg
//
LOCUS       DATMSK010000204         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_15284,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000204 DATMSK010000000
VERSION     DATMSK010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15284"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VNZ84_06720"
     CDS             complement(<1..442)
                     /locus_tag="VNZ84_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU66688.1"
                     /translation="MQTALYIQSDIAPQSPVFDVEIGYSAAKFSEETWKSLMKDDLEG
                     YRLHLAFDSANVQGFKTGYIAVKKNQVIVCLAPFFITDYNLDTTVQGKLKTLLQKVRQ
                     KIPFLMRLKILCVGSPVTDTCKMSMHPDYLYDPDIMQVLNEQLQK"
     gene            complement(446..1678)
                     /locus_tag="VNZ84_06725"
     CDS             complement(446..1678)
                     /locus_tag="VNZ84_06725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HWU66689.1"
                     /translation="MARRVVITGAGIVSPLGNDMQTFQHNVLAGVSGIGRLKADFCDQ
                     LETRIAAQCQFQGEQHFTRQELALLDRVSQFALYATEQAVQQSGLDFSSLNPTAIGCF
                     IGTGMAGSSSTEEGYSRLFREGLNRLKPFTVLMAMNSAAASQIATKYGVAGPNLTFST
                     ACSSAAVAIGEAAKQIAFGRCEVAIAGGSEALLTFGTIKAWEALRTLAKEDEQDAAAS
                     CKPFSANRTGLVLGEGAATFVLETYEHAVQRGAVILAEIVGYGSSNDFSHMTQPSVQG
                     QALSMQNALNDAGLRADQIGYINAHGTGTQLNDVTETKAIKQVFGPAAMQTPISSTKS
                     MHGHLMGAAAAVELSAVLVALHTNTLPPTMHLETPDPECDLNYVPNQAQQVAPLAYVM
                     SNSFAFGGTSGVLILRKI"
     gene            complement(1684..1929)
                     /locus_tag="VNZ84_06730"
     CDS             complement(1684..1929)
                     /locus_tag="VNZ84_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWU66690.1"
                     /translation="MDNTFEVVSELIANKLELDQKTITPQSKLTELGLDSLDIFDIIF
                     QAEDKYGIKVPNPSEEITTVEDVVNMLNNLLVAGKTA"
     gene            complement(1939..>2701)
                     /locus_tag="VNZ84_06735"
     CDS             complement(1939..>2701)
                     /locus_tag="VNZ84_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWU66691.1"
                     /translation="GTPAEMKHYGKQIARKGMSVVCPVLAGHCASVNELGKTSWQQWL
                     ASIEAVFLEMRKHYKTVFVSGLSMGALIALLLAAKYPTEVAGVILLSVTFFYDGWNIP
                     AFKQRFLLPLVLYSPLRYFMQWEETAPYGIKCERTRAMVAAVLENKDALTSEKIGYFK
                     TPATVIYQSVKLIQAARKCLPHVSVPVLIVHSTEDDMASLKNAYVVEKEVSSPRIDTF
                     YIGDTYHVVTLDKRKDDIAKRSAEFCLSVIKEANS"
BASE COUNT          632 a          718 c          686 g          665 t
ORIGIN      
        1 ctttttgcaa ctgctcattc aagacctgca tgatgtcagg gtcataaagg taatccgggt
       61 gcatgctcat tttgcaggta tcggtaacgg gtgaacccac gcataaaatt ttgaggcgca
      121 ttaaaaacgg gattttctgg cgcacttttt gcaacagcgt tttcagcttg ccttgcaccg
      181 tggtatcaag gttgtagtca gtgataaaga aaggcgccag gcagacgatg acctgatttt
      241 ttttgactgc gatatacccg gttttaaacc cttgtacatt ggcggaatca aaggccaggt
      301 gcaggcgata accttccagg tcatccttca tcaggctttt ccaggtttcc tcagaaaact
      361 tggcagcgct gtagccgatc tccacatcaa acacaggcga ttgtggagcg atatcagatt
      421 gaatatataa agccgtttgc atgatttaga tcttcctcaa aataagcaca ccactggtgc
      481 cgccaaaggc aaatgaatta ctcatgacgt acgccagcgg cgcaacctgt tgagcctgat
      541 tcggcacata attcaggtca cattctgggt ctggcgtctc aaggtgcatg gtgggtggca
      601 aggtgttggt atgcagtgca accagcacag cggagagctc aacggctgcc gcggccccca
      661 ttaaatgccc atgcatggat ttggtggagc taatgggtgt ctgcatcgcc gccgggccaa
      721 acacctgctt gatcgccttg gtttccgtga catcgtttag ctgtgtgcct gtgccgtggg
      781 cattgatata accgatctgg tcggcacgca agccggcatc attcaaggca ttctgcatgg
      841 ataaagcctg cccctgcacc gaaggctggg tcatatggct aaagtcattc gatgagccat
      901 atccaacaat ttcagccaga atcaccgcac cgcgctgcac ggcatgctcg tacgtttcga
      961 gcacgaatgt ggctgcgcct tcgcccaaca ccagcccggt gcggttggcc gagaacggct
     1021 tgcaggaggc tgctgcatcc tgctcatctt ccttggccag ggtacgcagg gcctcccagg
     1081 ccttgatggt gccaaaggtc agcaatgctt cactaccacc ggcaatcgcc acctcgcacc
     1141 ggccaaatgc aatctgtttt gctgcttcgc caatcgccac tgccgctgaa gaacatgctg
     1201 tagaaaaagt caggttaggc ccggcgacgc catatttggt ggcaatttgt gacgcagctg
     1261 cactgttcat ggccatcagt accgtaaacg gttttaagcg gtttaacccc tcacggaaca
     1321 gccgggagta accttcctcc gtggagctgg agccagccat gcccgtaccg ataaaacaac
     1381 cgatggcggt tgggttcagg ctggaaaagt ccaggccgga ttgttgtact gcctgctcag
     1441 tcgcatataa ggcaaactgg ctgacgcgat ccaataaggc caattcctgg cgggtgaaat
     1501 gctgctcgcc ttgaaactgg cattgcgcgg caatgcgtgt ttccagttgg tcgcagaaat
     1561 ctgccttcag gcggcctatg ccggacaccc ccgccaggac attgtgctgg aaggtttgca
     1621 tgtcattacc gagcggggac acaatgcccg caccggtaat gaccactctt ctggccatgc
     1681 tcattacgca gtcttgccag caacgagtag gttattgagc atattcacca catcttctac
     1741 agtggtgatt tcttccgacg ggttaggcac tttaatgccg tacttgtctt ctgcctggaa
     1801 aataatgtca aaaatatcca gcgagtccag tcccagctcg gtcaacttgc tttgcggagt
     1861 aatcgttttc tgatcaagct ccagcttgtt ggcaatcagt tcgctcacga cttcaaaggt
     1921 gttatccatt ttgtttcctt agctattggc ttctttaatc actgataaac aaaactccgc
     1981 gctgcgtttt gcaatatcat ccttgcgctt atccagcgtg accacatggt aggtatcgcc
     2041 gatataaaaa gtatcaatgc gcggactcga gacttccttt tccacaacat aggcattttt
     2101 caggctggcc atgtcatcct cggtggaatg gacgatcagc acagggacag agacgtgggg
     2161 cagacatttg cgtgccgcct ggatcagctt gacgctctgg taaatgacgg ttgccggggt
     2221 cttgaaataa ccgatttttt cagaagtcag ggcatccttg ttttccagca cggcagccac
     2281 catggcccgc gttcgctcac atttaatgcc ataaggggcc gtttcttccc actgcatgaa
     2341 atagcgtagc ggcgagtaca acaccagcgg caacaggaag cgctgcttga atgcaggaat
     2401 attccagccg tcataaaaga aggtgactga taaaaggatc acgccagcca cttcagttgg
     2461 atattttgct gcgagcagca atgcgataag cgcccccatg gagagcccgg aaacaaagac
     2521 cgttttgtag tgcttgcgca tttcgaggaa cacggcctca atcgaagcca gccattgctg
     2581 ccagctggtt ttacctaatt cattgacgga cgcacaatgt cctgccagta ccgggcaaac
     2641 caccgacatc ccttttcgtg caatttgctt gccataatgc ttcatttctg ctggcgtacc
     2701 g
//
LOCUS       DATMSK010000205        10494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_85485,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000205 DATMSK010000000
VERSION     DATMSK010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85485"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1877)
                     /gene="feoB"
                     /locus_tag="VNZ84_06740"
     CDS             complement(87..1877)
                     /gene="feoB"
                     /locus_tag="VNZ84_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HWU66692.1"
                     /translation="MKRVALLGMPNTGKSTLFNRLSGASARVGNWPGITVDLMSAKIL
                     LGGHIAELVDLPGLYDLHGFSDDEHVVRHFLSHHAVDLVLIILNSAQIDRQLSLALQI
                     RSLGLPAVLLLNMEDEAKRAGITIDTQAMSKQLGLPVRTISAKYGQGCPEALQLAAQT
                     MLQQPNLVTPQTIAGKLEMDNAIEQEMHQIIDRTVHFPLQLNHSLTDRLDKVLLHPWL
                     GLPLFLLSVFLLFQFIFTVGAPLQEGMGWLFDTLRSELLEPALTSLPTWLNGLLLDGL
                     YTGFSTVAAFVPIIVLFFLVMSMVEDSGYLSRAAFLMDTLMAKMGLDGRGFVMMLMGF
                     GCNVPALMGTRVMRSRPMRLLTMLTIPLSLCSARLQVFLFIIAILFPPSQAALVLFSL
                     YMVSFATIFITAMLFKNKFASHEPFVLELPPYRFPTPQQIWMRGWQEVKHFLSRATKF
                     IVIGVIMVWLLTHLPTTAVPASPETWAGSIGRFFSPVLDPIGIDTELAIALIFGFVAK
                     EIVVGSLAVIYGLQGDALSHQIAANIDWVQGMSFMLFTLIYTPCLSTIATLKSESKSS
                     GFMWLSLVWSLGLAWVVSFAFYQSARALGY"
     gene            complement(1874..2125)
                     /locus_tag="VNZ84_06745"
     CDS             complement(1874..2125)
                     /locus_tag="VNZ84_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HWU66693.1"
                     /translation="MHLDALKPGQTAVIDRIHAEQALSQRLGALGFRAGKQLEVIRQA
                     AFNGPLHVRIGSTDVIIRLQDAKAIHLHPLSHSTESTTA"
     gene            complement(2292..3101)
                     /locus_tag="VNZ84_06750"
     CDS             complement(2292..3101)
                     /locus_tag="VNZ84_06750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWU66694.1"
                     /translation="MKFLKVMLAIACFSALPHVYAADADAVATVNGKPIKKSWVDFIL
                     KDAEARTGQKANDGMKAAVVNELVGSELAYQEAQKAGIDKQPDFIASEELARRKLLVN
                     AFLADFIRKNPVTDAEKKEGYEQYKKELGDKEYNARHILVKTEAEAKAIIADLKKGTD
                     FAKIAKEKSQDPGSKDKGGDLGWFSPAGMVKPFAEALVGLKKGETTPEPVQSQFGWHV
                     IKLVDTRALQAPPYEKVQEGIERTLQQRKLEKYMLGLKDKAKIDVSGLEKK"
     gene            complement(3157..3420)
                     /locus_tag="VNZ84_06755"
     CDS             complement(3157..3420)
                     /locus_tag="VNZ84_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HWU66695.1"
                     /translation="MLRSEIEQRLQALAPEALEIQDDSELHRGHAGNTGGGHYTIKVK
                     SSLFSGKSQIMRHRMVYQCLQDLMPHRIHALSIQALSTDESFV"
     gene            complement(3421..3720)
                     /locus_tag="VNZ84_06760"
     CDS             complement(3421..3720)
                     /locus_tag="VNZ84_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWU66696.1"
                     /translation="MLYMIYAQDTPNSLPLRLEHRPPHLARLQQLQSEGRLILAGPLP
                     AIDSIDPGEAGFSGSLIVAEFDSLAAATAWANDDPFVHAGVYGQVVVKPFRKTLP"
     gene            complement(3737..4282)
                     /locus_tag="VNZ84_06765"
     CDS             complement(3737..4282)
                     /locus_tag="VNZ84_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HWU66697.1"
                     /translation="MQVITDLLPVILFFIAYKKAGIFTATAVAMISTLVLMLFIWIKR
                     GKIDKMLMINGAVITVLGGITLLLHDKTYIMWKPTAIYWIGALALLFSRYLFKRNLIE
                     SMLGKMMAPPLAIWDRLNLCWIAFLVGMGILNLYVAFHFSEDDWVNFKLFGATSLMFI
                     FIIAQVFALKDHWIEQNTPSV"
     gene            4412..5263
                     /locus_tag="VNZ84_06770"
     CDS             4412..5263
                     /locus_tag="VNZ84_06770"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HWU66698.1"
                     /translation="MSVPIDLHCHSTMSDGLLTPEELVAHAASKGVRVLALTDDDEVS
                     GLARARSAAEQLGISFVNGVEISVTWKKRTLHVVGLRVDPTHPALLETFARVREGRND
                     RARQIAEGLAKAGIGGAYEGAREIAGNSVMTRSHFAQFMVKHGHAKNIKSVFKKYMVK
                     GKPGYVNHEWMSLEEALTLIRNSGGVAVLAHPGRYDLGFVNMHLLLHEFRNLGGAAIE
                     VVTGSHQPPQYDQFAKLAHRFDLKASQGSDYHGPGMSYMEMGRLPALPSGCIPVWQDW
                     PEASQLQ"
     gene            5270..5881
                     /locus_tag="VNZ84_06775"
     CDS             5270..5881
                     /locus_tag="VNZ84_06775"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWU66699.1"
                     /translation="MAQYFIIHAENPQARLIQQAVQLLKQGGVLACPTDSSYALVCLM
                     DYKDAQVRIRRLRGVDDEHPFTLICRDLAEIGTYAKVNNSQFRLLKAMTPGAYTFLLE
                     ASREVPRRLQHPKRSTIGLRVPRHVVTQALLEALDSPMLSMTLQLPRDEAPMSVGWEI
                     RETLEHQIDAVIDSDIVHVGATTVLDLTQDPPELVRAGVAPWP"
     gene            5903..6544
                     /locus_tag="VNZ84_06780"
     CDS             5903..6544
                     /locus_tag="VNZ84_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWU66700.1"
                     /translation="MEELTVVQKIAAYALPILFAITVHEAAHGYAAKYFGDLTAERMG
                     RITLNPFKHIDPIGTILLPALSIMLGGVLFGWAKPVPVNFGQLRRPKQDMLWVALAGP
                     ASNFVMALFWGLLLARVGLFPESAQAYLAQMSLAGVQINLVLLVLNLFPLPPLDGGRI
                     AVSLLPMKAAIKFAQIERYGMFILIALLFTGILGILLSPVLRFFQQLLSILIM"
     gene            complement(6581..10243)
                     /locus_tag="VNZ84_06785"
     CDS             complement(6581..10243)
                     /locus_tag="VNZ84_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWU66701.1"
                     /translation="MLNRLRSNFSHLNWSRVSQSLVVRIAAGLIVFYFLFAWFAVNPL
                     AKWLVPWVAEKQLASKASVEKVTFDPLRLKTTIEKLSLTEKNGKPLASFDSLVVDLEA
                     SGLLDWAWKLKQITLTAPYVNVHISKQGRLNWADLIAKLNENPSPPEQDLPRVIIENI
                     AIHQGNVAYLDDHQATALQASLKPLDFELEGFSTLPKDRGDYLITAKLPAQGGTLKWK
                     GNFGVNPLASQGALAIEGVQIAKLMEWVNPAALPVQVQAGTFAGKLNYDFSLVNQHPK
                     LLVQDTSLNINNLAGKLAQGDQLKLDQLAFNTKRLVLDQQTQLAVTTDGIDIQLNQLT
                     LHKPQSFLSIASTRLALPKLVFNLDKEAQLLFDHLTLTLQHAQVQHAQQTLLQLPELS
                     IENASFNLAERQADIARIQLSKGQLSATQTASGKLDWQQVFESPATAPASIAKVEPEP
                     VNEQSSQPFHFSIHELAFAHWQLSYTDQGFIKPLQTMVNDLNLQLAVDNTSGLALKNM
                     QLDASQLSLQSDKKPAARLAKLAVSDMRLALEQQKADISAIQLIGLNTAVIRQADQQL
                     NWQTMLARATGKQTAHAAPASHTATHTNPWSYRLKRLALQQADLHIEDQSTPRPVLLD
                     ITEGNIELADLSQDMSRLLPIRTGFKIKQGGQFKLQGKLAASPFKMEAQSALTDVSLK
                     PFAPYIEQVALLRLENGSASLQGKLQQTPSGATKFDGGFSVDQLSIIEEDSQQPFLRW
                     EKLQGEGLALSLAPNTLQVSTLTLSKPQTKFIIYPDRSLNISKLMRTPSSAGSPAVEG
                     TPSSAPSTASKNISQTKAASNPQSTDFPVAIDSVRINNAELEFADLSLPQPFGTHIHT
                     LGGVINGISSNPSSTAQVELDGKVDDYGAARIRGSLQPFKTTEFTDIKLAFTNLEMNR
                     LTPYSGKFAGRKIESGKLSVDLEYKIKQRKLAGENKFVINKLTLGERVDSKDAPNLPL
                     DLAIAILEDSNGVIGLDLPVSGSLDDPKFSLGGIIWKAFTNVLTKIVTAPFSALGKLF
                     GGGEKLEAIVFDSGNATLLPPEMEKLKSVATALGKRQQLKLGIVPGYAVVADTRAIQE
                     ATLRQQVAEEMGVRLKTGEAPGPIDLNNPKVQSAIQALHDRLTNKGLLKRLAAKLEKT
                     PDGFYEQAQETLTTSIQVTEADLQSLSKARAMAIQKSLLESGVSTERVEISQPTTVKA
                     ERDHVPTKLTLEAMKR"
BASE COUNT         2440 a         2470 c         2782 g         2802 t
ORIGIN      
        1 attttaacag aaagcgcgac gtctcgcagg gctaatcaat aaaaacggcc acagttgagt
       61 ggccgtttgt gatgcagcta agcggactag taacccagtg cgcgtgcgct ctggtaaaag
      121 gcaaaactga ccacccaggc caatcccagc gaccaaacga gtgatagcca cataaaacca
      181 ctgcttttac tctcggattt gagcgtggca atggtagaca gacagggcgt gtaaatcagc
      241 gtgaatagca tgaaactcat gccttgcacc cagtcaatat tagccgcaat ctggtggctc
      301 aatgcatctc cctgtaagcc ataaatgaca gccagcgaac ccaccacaat ttctttggca
      361 acaaagccaa aaatcagggc aattgccagc tcggtatcga tgccgatcgg atccagcact
      421 ggtgagaaga agcgaccgat gctgcccgcc caggtctcgg ggctagccgg taccgctgtc
      481 gtgggtaggt gcgtaagcag ccagaccatg atcacgccga tgacaataaa cttggtagcg
      541 cgcgacagga aatgtttgac ttcttgccag ccacgcatcc agatttgttg tggggtaggg
      601 aaacggtatg gcggtagttc aaggacaaat ggttcgtggc tggcaaattt gtttttaaac
      661 agcatggccg tgatgaaaat agtagcaaag ctcaccatat agagtgaaaa cagtaccagc
      721 gcagcttgtg agggcgggaa caggatggca atgataaaca ggaacacctg taatctcgca
      781 gaacacagag acagcgggat ggtcagcatg gtcagcaggc gcatgggacg gctacgcatg
      841 acgcgggtgc ccatcagggc aggcacattg cagccaaatc ccatgagcat catgacgaag
      901 ccgcggccat caagccccat tttagccatc agcgtatcca tgaggaaggc tgcgcgtgac
      961 aagtagccac tgtcctccac catgctcatg accaggaaga acaacacgat aattggcaca
     1021 aacgctgcca cggtgctgaa gccagtatac aggccatcga gcagcaaacc gtttaaccag
     1081 gtcggtaagc tggtgagtgc gggttcgagc aattcgctgc gcagggtatc aaacagccaa
     1141 cccatacctt cttgtaacgg agcgcctacc gtgaaaataa actggaacag caggaatacc
     1201 gatagtaaaa agagcggtag gcccaaccag gggtgtaata gcaccttatc taggcggtca
     1261 gtgagtgagt gattaagttg cagcgggaaa tgcaccgtgc ggtctatgat ctggtgcatt
     1321 tcctgctcaa tggcattatc catttccagt ttgccggcaa tggtttgcgg ggtaacgagg
     1381 ttaggttgtt gcaacatggt ttgtgccgcc agttgcaagg cttctgggca cccttgcccg
     1441 tatttggcgc tgatggtacg cacaggcagg cctagttgct tgctcatggc ctgcgtgtct
     1501 atggtgatgc ctgcgcgctt ggcctcgtct tccatgttga gcagcaatac tgcaggcagg
     1561 ccaagtgagc ggatttgcag tgccagtgac agttgtcggt caatctgggc gctgttgaga
     1621 ataattaata ccaaatccac ggcatgatgg ctcaggaagt ggcggacgac gtgttcatca
     1681 tcagaaaagc catgcaggtc atacaggccg ggtaagtcca ccaattcggc aatatggccg
     1741 cccagcaata tcttggcact cattaagtcg accgtgatgc caggccagtt acctacacgg
     1801 gcagaagcac cgctcaatcg attgaacagg gtggatttgc ccgtattggg catcccgagt
     1861 aaggcaacgc gtttcatgct gtagtagatt ctgtagagtg tgacagtgga tgcaagtgga
     1921 tcgctttggc gtcctgcagg cggatgatga catccgtaga gccgatgcgc acatgcaatg
     1981 ggccattaaa agccgcttga cggattactt ccagttgttt gccagcacgg aagccaaggg
     2041 cacccaggcg ctggcttaag gcttgctctg catggatgcg atcaatcaca gcagtttggc
     2101 ctggtttgag tgcgtcaaga tgcatggtgg ttttataaat gagaataatt cttgatttta
     2161 tcttagattg caggacaaag aaacggttct gtgccgacct gcaataacgt aagacttcgt
     2221 tcgagtgatt ttggggcaat aaaaaagcca gtctagactg gcttttttat ttaaatacgt
     2281 caaatgcaag cttatttttt ctcgaggcca ctgacgtcaa ttttggcttt gtctttcagg
     2341 cccagcatgt atttttcaag cttgcgttgt tgtaatgtac gctcaatgcc ttcctgtact
     2401 ttttcgtatg gaggcgcttg tagggcacgg gtgtcaacca gcttgatcac atgccagccg
     2461 aactgggact gtactggctc aggcgtggtt tcgccttttt tcaggccaac cagtgcttcc
     2521 gcgaatggtt tgaccatgcc ggcaggagag aaccaaccca gatcgccacc tttgtcctta
     2581 gagcctggat cctgagattt ttctttggca atcttggcaa agtcagtgcc ttttttcagg
     2641 tcagcaataa tggctttggc ttcagcttct gtttttacca ggatgtgacg tgcattgtat
     2701 tctttgtcac ccagctcttt tttgtattgc tcgtagcctt cttttttctc ggcatcggtg
     2761 acaggatttt tcctgatgaa gtcagcgagg aaagcgttga ccagcaactt gcggcgggcc
     2821 agttcttcac tggcgataaa gtctggttgt ttatcgatac ctgctttttg cgcttcctgg
     2881 taagcgagct ctgagcccac cagttcattg accacggcag ctttcatgcc gtcattagct
     2941 ttctgtccgg tacgtgcttc agcatctttc aaaatgaaat ctacccagga ttttttaatt
     3001 ggcttgccgt tgacggtggc aaccgcatcg gcatccgccg catacacatg tggcagcgcg
     3061 ctaaagcaag caatcgccaa cattactttc aaaaatttca tgcaatatcc ttatataacg
     3121 atttgaattg gtgcgatatt aaaaaacagg attgtttcaa acaaatgact catcagtcga
     3181 taaggcctga atactgagcg cgtgtatgcg atgaggcatt aaatcttgca aacattgata
     3241 gaccatgcga tggcgcatta tctgtgattt tcccgaaaat agcgagcttt ttaccttgat
     3301 ggtgtagtgc ccgccgcctg tgtttcctgc gtgtccgcgg tgaagctcac tgtcatcttg
     3361 aatttccaga gcttcagggg ccagtgcctg taaacgttgt tcaatttctg agcgtaacat
     3421 ttatggcaag gtttttctaa atggtttgac cacgacctgc ccataaacgc cagcatgtac
     3481 gaacgggtca tcatttgccc acgccgtggc agccgctaaa gaatcaaatt cggccacaat
     3541 caggctaccg ctaaagcccg cctcgccagg gtctatgctg tctatggcgg gtaatggacc
     3601 tgccaatatc aagcgacctt cactttgcaa ttgttgtagt cttgctaagt gcggtgggcg
     3661 gtgctccagc ctcaatggca ggctgttggg tgtatcttgt gcataaatca tgtaaagcat
     3721 ggcagttttc tcttagtcag actgaaggcg tgttttgttc aatccagtga tcttttaagg
     3781 caaatacctg cgcaataata aagatgaaca tcaggctggt ggcaccaaac aacttgaaat
     3841 tgacccagtc atcttcgctg aagtggaacg cgacatacaa gtttaaaatg cccatgccga
     3901 ccaggaatgc aatccagcac aggtttagcc tgtcccagat ggctaatggt ggagccatca
     3961 ttttgcctaa catgctttca atcaggttgc gtttgaatag ataacggcta aacagcagtg
     4021 ccagtgcgcc tatccagtaa atggccgtgg gtttccacat gatgtaggtt ttgtcatgca
     4081 gcaataaggt gattccgccg agtacagtaa tcaccgcacc attgatcatc agcattttgt
     4141 ctatcttgcc gcgtttgatc cagataaata acatcagcac cagtgtgctg atcatggcca
     4201 ccgccgtggc ggtgaaaatc ccggcttttt tataggcaat aaaaaagagg atgacgggca
     4261 ataaatcggt aatgacttgc atgactggct tcaggcgact gaattcctgc tatctgtaag
     4321 ggcactattt tgctatgatt tctgtcgaaa tgtaaaggtt gcctgtttta ctgcatatcc
     4381 cttctaatca tcaatgtcat cattatccat catgtcagtc cccattgatt tacattgtca
     4441 ttcaaccatg tcggatggct tacttacccc ggaggagctg gttgcgcatg ctgctagtaa
     4501 aggcgtacgc gtgcttgcgc tcactgatga tgacgaagtg agcgggttgg cgcgtgctcg
     4561 ttctgctgcc gagcaactgg gtatatcttt tgtgaatggt gttgaaatct cggtgacctg
     4621 gaaaaaacgt accttgcatg tggttggttt gcgggtagac cctacgcatc cagccttgct
     4681 ggaaaccttt gccagggtgc gagaaggccg caatgaccgc gcaaggcaga ttgctgaagg
     4741 cttggccaag gccggcattg gtggtgctta tgaaggtgcc cgtgaaattg ccggcaacag
     4801 tgtcatgacc cgcagtcatt ttgcccagtt tatggtgaag cacgggcacg ccaagaacat
     4861 caaatcagta tttaaaaaat acatggtaaa gggcaagccc ggttacgtaa atcatgagtg
     4921 gatgagcctg gaagaggcgt tgacactgat acgtaacagt gggggcgttg ctgtgctggc
     4981 acacccgggc cgttatgacc tgggatttgt aaatatgcat ttgctattgc atgagttcag
     5041 gaatctgggc ggtgcggcaa ttgaagtggt gaccggcagc catcagcccc cacaatatga
     5101 ccaattcgcc aagctggcac accggtttga tttaaaagct tcacaagggt ctgactatca
     5161 tgggccaggc atgtcttata tggaaatggg gcgcttgcca gccttgccgt caggatgcat
     5221 ccctgtctgg caggattggc cggaagccag tcaattacaa taatcattta tggcacagta
     5281 tttcattatt catgcggaga atccccaggc aagattgatc cagcaagcgg ttcagctgtt
     5341 aaagcagggc ggggtgctcg catgccctac ggattccagt tacgcattgg tatgcctgat
     5401 ggattataaa gatgcacaag tgcgtatccg ccgtttacgt ggtgtcgatg atgagcatcc
     5461 atttaccctg atttgtcgcg acctggccga gattgggacc tatgccaaag tcaataatag
     5521 ccagttccgg ttgctcaagg cgatgacacc tggtgcgtat acgtttttac ttgaagccag
     5581 ccgggaggtg ccacgacgct tgcaacaccc caaacgtagt accataggcc tgcgggtgcc
     5641 caggcatgtg gtgacacaag ctctgttaga ggcactggac tcgcctatgc tcagtatgac
     5701 tttacagctg ccgagagacg aagcgcccat gtcagtgggg tgggaaatcc gcgaaaccct
     5761 ggagcaccag attgacgcag tgatagatag tgatattgtg catgtgggcg cgaccaccgt
     5821 gctggattta acacaagacc cacccgagct ggtacgcgct ggtgtggcgc cctggcctta
     5881 atgttttttg gaagtatttt gcatggaaga actcaccgtc gtacaaaaaa tagctgctta
     5941 tgccctgcct atcctgttcg cgatcacagt acatgaagcc gcgcacgggt atgccgccaa
     6001 atattttggt gacctgaccg cagagcgcat gggaaggatc acgctcaatc cattcaagca
     6061 tattgatcca atcgggacca tcctgttgcc tgccttgagt attatgcttg gtggtgtgtt
     6121 gtttggctgg gccaaaccgg tcccagtcaa ttttggccaa ctgcgccgtc ccaagcagga
     6181 tatgttgtgg gtggcgctcg cgggtccggc ctccaatttt gtgatggcct tattctgggg
     6241 attgttactg gcaagagtgg ggttgtttcc cgaatctgca caagcttacc tggcacaaat
     6301 gagcctggct ggcgtgcaga ttaatctggt tttgctggta ttgaatttat ttccattgcc
     6361 accgttggat ggcggacgta tcgcggtgag tttattgccg atgaaagcag caatcaagtt
     6421 tgcccagatt gagcgttacg gcatgtttat cctgattgct ttgctgttta ccggcattct
     6481 gggcatttta ttatccccag tgctccggtt tttccagcaa ttgctaagca tactcattat
     6541 gtaagcttat aggcgcagcc gcaagctgcg ccacttacga ctagcgtttc atcgcttcca
     6601 gggtgagttt ggtcggtaca tggtcacgct ctgccttgac cgttgttggt tgcgatattt
     6661 ccacacgctc ggtactcacg cctgattcaa gcaaggattt ctggatagcc attgcacgcg
     6721 ccttagacaa gctttgcaag tcagcctcag tgacctggat gctggtcgtc aatgtctcct
     6781 gtgcttgttc ataaaaacca tcgggtgttt tctccagttt ggctgccagt cgtttgagca
     6841 agcctttgtt ggttaaacgg tcatgcaggg cctgtattgc cgattgcact ttgggattgt
     6901 tcaggtcaat cggccctggc gcttcaccag ttttgaggcg tacgcccatt tcttcagcca
     6961 cttgctggcg cagcgttgct tcctggatgg cgcgtgtgtc tgctacgaca gcatacccag
     7021 gcacaatgcc cagttttagc tgctgtcgtt tgcccagtgc agtagcgaca gactttagtt
     7081 tttccatctc gggtggcaaa agggttgcat tgccggaatc aaatacgatc gcttccagtt
     7141 tttcaccgcc accaaacagt ttgcccagcg cactaaatgg cgcggtgacg attttggtca
     7201 atacattggt aaaggctttc cagataatgc cgccaagact gaacttcgga tcgtccaggc
     7261 tgccgctgac aggcaagtcc aggccaatca caccattgct gtcttcgagg atggcaatcg
     7321 ccaggtccag tggcaaattg ggggcatcct tgctgtcgac acgttcccca agcgtcagtt
     7381 tgttaatcac gaatttattt tcgcccgcca gttttcgctg ttttattttg tattccagat
     7441 cgactgacag tttgcctgac tcaattttcc ttccggcaaa tttgccagag taaggcgtta
     7501 aacggttcat ttccaggttg gtaaatgcca gcttgatatc cgtaaattcg gtggttttaa
     7561 atggctgcag tgaaccgcgg atacgcgccg caccataatc atcgacctta ccgtccagct
     7621 cgacttgtgc cgtggaagac ggattgctgg aaatgccatt gatgacgcca cctagtgtgt
     7681 ggatatgcgt cccaaaaggt tgtggtaacg ataggtccgc aaactccagt tccgcattgt
     7741 tgatgcgtac actatcaatt gcgaccggga aatcagtgct ttgaggattg ctggcagcct
     7801 ttgtctggga gatgttttta ctcgccgtgg aaggggcgga tgatggagtc ccttccacgg
     7861 ctgggctgcc cgctgacgac ggtgtacgca ttagcttgct aatattcagg cttcggtcag
     7921 gataaataat gaatttggtt tgaggcttgc tcagggtcag tgtgctgact tgcaatgtat
     7981 taggcgcgag tgaaagcgcc agtccttccc cttgcaattt ctcccagcgc agaaagggtt
     8041 gctggctatc ctcttcgata atggataact ggtcgacgct gaatccgccg tcaaatttgg
     8101 tggcgccact gggtgtttgc tgcaatttcc cttgcaggct ggctgagcca ttttccaggc
     8161 gtaacaaggc gacttgttcg atataaggcg cgaacggctt caaggataca tcagtcagtg
     8221 cactctgtgc ttccattttg aagggtgacg cggcgagttt gccttgtagc ttgaactgac
     8281 cgccttgctt gattttgaag cctgttctga ttggcaacaa ccgtgacatg tcctggctta
     8341 agtcagcgag ttcaatattg ccctcggtaa tatccagtaa gacaggtctg ggcgtagact
     8401 ggtcttcgat atgaagatcg gcctgctgta aagccagtcg cttcaagcgg tatgaccatg
     8461 ggtttgtgtg ggttgccgtg tggctggccg gggcagcatg cgcagtctgt ttgccggtgg
     8521 cgcgcgccag cattgtctgc cagtttaact gctggtcagc ctgccggata accgcagtat
     8581 taagtcctat cagttgaatg gcggaaatgt ctgctttttg ctgttcgagg gccagtcgca
     8641 tgtcactcac cgcaagcttg gcaaggcggg cggctggttt cttgtccgat tgtaaactga
     8701 gctgtgaggc atccaattgc atgtttttca gtgccagacc tgaagtgtta tccactgcca
     8761 gttgcagatt aaggtcattg accatcgttt gcagtggctt gatgaatcct tggtcggtat
     8821 aagacagctg ccagtgtgca aacgcaagtt catgtatgct gaagtgaaaa ggttggctgg
     8881 attgctcgtt aaccggttct ggctcaactt tcgcgatgga cgcaggtgca gtggctgggg
     8941 attcaaatac ctgttgccag tccagttttc ctgaggcggt ttgcgtggca gagagttgcc
     9001 ccttacttaa ctggatacgt gcgatatccg cttgtcgttc agccaaatta aaactggcat
     9061 tttcgatgga cagttccggc agttgtaata gtgtttgctg ggcatgttgc acttgtgcgt
     9121 gttgcagggt gagggtgagg tgatcaaaga gtagttgtgc ttccttatcc aggttgaata
     9181 ccagtttggg taaggcaagt cttgtggagg caatcgatag gaatgattgt ggtttatgta
     9241 atgtgagttg attcagctgt atatcaatgc catctgttgt gaccgcaagt tgcgtctgct
     9301 ggtccagtac caggcgtttg gtattgaatg ccagttggtc cagtttcagt tgatctcctt
     9361 gagccagttt accagccaaa ttattgatgt tcaggctggt atcttgcacc agcagtttgg
     9421 gatgttgatt aaccagggag aaatcatagt tgagtttgcc agcaaacgtc ccagcctgga
     9481 cctggactgg cagcgcggcc ggattgaccc attccatcag cttggcgatt tgcacgcctt
     9541 cgatggctaa tgccccttgg gaggcaagtg gattgacgcc gaaattgcct ttccatttta
     9601 atgtgcctcc ctgagctggc aattttgcag taatcaggta gtcgcccctg tctttgggta
     9661 atgtggaaaa cccttccagt tcaaaatcca gtggtttcag cgaggcttgc aatgcggttg
     9721 cctgatggtc atccagatag gcgacattcc cttgatggat cgcaatgttt tcgatgatca
     9781 cacgtggcaa atcttgctct ggcggggaag ggttttcgtt aagcttggcg atcagatctg
     9841 cccagttaag acggccttgt ttggaaatat ggacattgac gtatggggct gtcagtgtga
     9901 tctgtttcag tttccaggcc caatccaata atccgctcgc ctccaggtcg acgaccaaac
     9961 tgtcaaatga ggccagaggc ttcccatttt tctcggtgag tgacagtttc tcgatggtag
    10021 ttttgaggcg taaaggatca aacgtgactt tttctacact ggctttactg gccaattgct
    10081 tttctgcaac ccatggcact aaccatttcg ccagtgggtt gaccgcgaac caggcaaata
    10141 aaaaataaaa tacgatcagt ccggcagcaa tcctcacgac cagggattgg gatacgcgag
    10201 accaattgag atgggaaaag tttgagcgaa ggcggttcag cataggcgtt aatttatcag
    10261 aatttaaatg tttataccgt tgttgatgct acggataata caaatcattg ctgcaaatga
    10321 ggcgtctatt ggcttgaagg tgacattgcc atcatttttc atgtatgctt agatcaacac
    10381 gttacacagg atgctgtttt cagcagtaaa tatggcttca gagccggata agttgtctca
    10441 caaaccctct cttttagaac gattaagcca ttttcttctg cgcgagccag aaga
//
LOCUS       DATMSK010000206        15852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_156056,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000206 DATMSK010000000
VERSION     DATMSK010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 15852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15852
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156056"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VNZ84_06790"
     CDS             complement(<1..200)
                     /locus_tag="VNZ84_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWU66702.1"
                     /translation="MTQAYKIPVSALILIHTPDLQVLLLERADKAGFWQSVTGSTEAG
                     ETPYDAAIREVKEETGLDALAY"
     gene            349..1719
                     /locus_tag="VNZ84_06795"
     CDS             349..1719
                     /locus_tag="VNZ84_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWU66703.1"
                     /translation="MLRRQRGWACGMLMVYAQAISAGEATSARGQFVPPEPGAVQSSF
                     NQALLGQLTLRQAEQLFASGNRELLAAKRAVEGAEANTLIAGQRPNPVLSLGLSSVNV
                     NRGQGNMNQNGANGVWDKTYNSALQVSQLYERGNKRELRTSVAESAVKAYGFDLKETY
                     RQQALAMAMAYYDLKLAQETLLIQQANVASYDNTLQAAQLRLKAGDVASADVARIRVD
                     ALRASNDLRQAEATLQKTQATLAYLLGKDQDASKLYASDPWPGLTSDQLTGAESAWLP
                     QRSDVQAAEARMEQAQQARKLAESLKTRDVTWSLAYQHFPGQEPGSAPDTVGASVSIP
                     LFTNYQYEGEAARAEVDYTSAMEAKEQVKAAAVAEMRRAKADLQAAVDKNRRFDQSIL
                     SEAQKAAEAAEFAYQHGAMSVMDLLDARRILRALQLESASVKADYAKSLSAWLAATRQ
                     AFTNQD"
     gene            1725..2879
                     /locus_tag="VNZ84_06800"
     CDS             1725..2879
                     /locus_tag="VNZ84_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU66704.1"
                     /translation="MTMPIFNHPLTFLASTKSRIALVLLTLLTGQAYAADKTVGNAQN
                     QAANEIVLTAGSPQLTSLRVEPVKEIPVPVTEPLNGKIVFDENRTARIFSPVAGRALS
                     IKAEVGDAVKVGQTLLVMDSPDVGGAVADVRKSQADLQLKQQALERSRMLVDHGVLAK
                     KEVEVAQADWASSRAESERATARLKNLGVSTAANDNYAVRSPIAGMVVDRKINPGSEV
                     RPDAADPLFIVTDPNYLWASIDLPERDLSRISQGQKLSIQVDAYPDEVFSGEVKTIGV
                     MVDPTTRRIPVRCMVESKGKLKPEMYARITPLDSSQHRVIRLPNSALITEGLYSYVFV
                     EERPLHFKKRKVTLDLQRREYATVKEGLKLGERVVVGGAILLNSDLSVSK"
     gene            2916..6008
                     /locus_tag="VNZ84_06805"
     CDS             2916..6008
                     /locus_tag="VNZ84_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWU66705.1"
                     /translation="MLKGLITFSLQQRVFLLIGAVVLTIFGWAAVQNLPIEAFPDVED
                     VHVIIVTQSPGMAPEEVERVVSLPIEHGMSGVPGLSQIRSVSITGLSVVTLTFAERTE
                     DYFARQQVLERLQGVSLPSGLQPTLAPLTTAVGEVYRYLVEAPQDMPQREVRAIQDWK
                     IIPQLRMVPGVADITSFGGTVKEYQIKLDPYRLRKFGISIDQVNQAVTNNSSNVGGGL
                     MHRGDESLVVRGIGLFHNLDDIAQAVVDTRDGKPILVSDLGQVVIGDRPRSGIVSFNQ
                     QDDIIEGIVLMTKGQNAAKVIEALRQKVEVVNESLPAGVKIVPFYDRTGLVHHTVHTV
                     AENLIVGALLVVTILVVFLRNWRAALIVASVIPLSLLFSFIIMDLRGVSANLISLGAV
                     DFGIIIDSAVVLVEALMVRLAIAEDDHNPTHNGLAWRMQTLKNTAIDMGHPILFSKAI
                     IITAFLPIFTFQRVEGKIFSPMAFTLSFALLGAIILTLTLVPALLSYTMNREDMAEKH
                     SVWMHRLQQGYRNILLKGSQSRMLVVLASAAVLVVSVVSAPMLGSEFLPKLDEGNIWL
                     TVDLPPSASLEKTKEVERHIRKILNSYQEVRLVVAHVGRPDDGTDPKGPYHMEILADL
                     KPKSEWTFADKEALIKDMSRRIHEIPGVPTNFSQVIQDSVEEALSGVKGEIAVKIFGP
                     DLAILEQKATEVVDAMNTVRGATDVAAVRISGNSELDITLNRSQLSRYGLMVGDVNNT
                     VQTALAGNAANTFYDGDRHFDVTLRLDRPFRDSVDDIDELPIALPNGAGSIPLSEVAE
                     VSIKQGASRVSREAGARIVSIKANITDRDQGSFVKEAMQTVKAKVKLPPGYAMTWGGQ
                     FENQQRAMKRLMVIVPLSVLLIFVLLFWAFKSVKNALLVLLMIPFTLVGGLAGLAIAG
                     LHLSVSAAVGFIALAGISVQNGVIMVEQIKKLMREGQSVTESVIEGAVARLRPILMTA
                     LMAGLGLMPAALSHGIGSETQRPFAVVIVGGLITATLFTLLLLPLLFPLFSDVRRRKS
                     "
     gene            complement(6314..7294)
                     /gene="ispH"
                     /locus_tag="VNZ84_06810"
                     /gene_synonym="lytB"
     CDS             complement(6314..7294)
                     /gene="ispH"
                     /locus_tag="VNZ84_06810"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWU66706.1"
                     /translation="MMEFIMQTEVTPNIVLANPRGFCAGVDRAIIIVEQALEKFGAPI
                     YVRNEVVHNKFVVGQLRDKGAIFVDELEKIPAGSIVIFSAHGVSKAVRAEAESRGLTA
                     FDATCPLVTKVHIEVAKMRKDGLEIIMIGHKGHPEVEGTMGQSEAGGMYLVETPEDVT
                     ALQVADPEKLAYVTQTTLSVDDATQVINALKARFPNIKAPKSDDICYATQNRQDAVKI
                     MAKDCDLVIIVGSPNSSNSNRLREVAKNQGVEAYMVDNASHLKAEWLQGKQKIGISAG
                     ASAPEVLVKEVIAALQNMGAAQVEELQGVVENVVFQLPKNLVNAEKRTPG"
     gene            7397..8251
                     /gene="purU"
                     /locus_tag="VNZ84_06815"
     CDS             7397..8251
                     /gene="purU"
                     /locus_tag="VNZ84_06815"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWU66707.1"
                     /translation="MKNTATLLITCPDTKGIVAAIAQFLYEHNANILHADQHQDAENN
                     LFLMRVEWDLTNFNVVVADFEQAFGPVAQRFDMTWQLKLSEQKTRMAIMVSQYDHCLV
                     DLLHRHHSGELACEIPLIISNHRDTEKLAQFYGIPFHYLPMTKETKAEVEAQQFALFD
                     QHKIDLIVLARYMQILSPDFVARYPQRIINIHHSFLPAFIGARPYHRAFERGVKLIGA
                     TSHYVTEVLDEGPIIEQDLARISHRDQVEDLIQKGRDLERVVLSKAVRWHIEHRILLY
                     ANKTVIFD"
     gene            complement(8317..9561)
                     /gene="lysA"
                     /locus_tag="VNZ84_06820"
     CDS             complement(8317..9561)
                     /gene="lysA"
                     /locus_tag="VNZ84_06820"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWU66708.1"
                     /translation="MTAFSIQQGLLHAENVALRDIAQTHQTPTYVYSRAALTQAFERF
                     QAGLVGHDHLICFAVKANPSLAILNLFARLGAGFDIVSGGELARVLAAGGDPKKVVFS
                     GVGKSHAEIKAALEAGILCFNVESVNELDRIQQVAADLGKKAPVSLRVNPNVDAKTHP
                     YISTGLKNNKFGVAFEDALGLYEKAAQLPNIEVHGVDCHIGSQITELSPFLDALDKVL
                     GLVDALAAKGIHIQHIDVGGGVGITYSDETPPDFASYTAAILKKLAGRNVKVLFEPGR
                     ALVGNAGLLLTKVEYLKPGESKNFAIVDAAMNDLMRPALYDAFHNITTIAPSAAPAQI
                     YEIVGPVCESGDFLGHDRTLAIEEGDYLAIHSAGAYGMSMASNYNTRARAAEILVDGD
                     QAHVIREREQIADLFKLERTLP"
     gene            complement(9558..9695)
                     /locus_tag="VNZ84_06825"
     CDS             complement(9558..9695)
                     /locus_tag="VNZ84_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HWU66709.1"
                     /translation="MLSSWTRLLAIALLLAQLSACGLKGDLYIPERQYPQTPQQDKSS
                     S"
     gene            9862..10272
                     /locus_tag="VNZ84_06830"
     CDS             9862..10272
                     /locus_tag="VNZ84_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HWU66710.1"
                     /translation="MKHSNQFGFSLIEVMVVVAIMGILASIAIPGYQDYIKSGNAAEA
                     PANLANCRVQAEQFYQDNFTYVGFACVPSDAKFFDYSIDNQSATTYTLKATGKSGQNM
                     GNFSFTVNQDNAKTSTFDGTTGSTCWLTSSSGSC"
     gene            10275..10787
                     /locus_tag="VNZ84_06835"
     CDS             10275..10787
                     /locus_tag="VNZ84_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HWU66711.1"
                     /translation="MRQKGFTLVELVVTMVVLVAVSSIAIPAFQTAIGNAQIRTVAES
                     IRDGLQQARVEAIKRNARVKFTLQTNSAWQVGCVTQTTACPTLITQKSASEGSSSNTT
                     VAADNYTAVFSSFGTRDPATPAGLSVVNITNGQVKAEERKALRVMLAAGGNVKVCDPS
                     VTTVGDPRAC"
     gene            10811..11188
                     /locus_tag="VNZ84_06840"
     CDS             10811..11188
                     /locus_tag="VNZ84_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66712.1"
                     /translation="MTFTSMQQGSVLIEAMVALVIFSMGVLALVGLQSAMIKNSSDNR
                     YRAEAQLIAQTHIANMMAYGGDAANYITQVDKDKIKSQLPNGTLTFSALTNTMVTVTV
                     GWQVPGGNPHQVNASSYLFDVMP"
     gene            11185..12342
                     /locus_tag="VNZ84_06845"
     CDS             11185..12342
                     /locus_tag="VNZ84_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HWU66713.1"
                     /translation="MMKQSTWTLARLHRPRFQAGLSLVELLIGLLIGLVATLAISNLF
                     SGFEARKRMIAGGADAQSSGVLAMYYMQRDAQNAGYGLPLYNSSDPSPLLCPIDTSIN
                     QGGVVINLSPVIIVDGGTGNDIVRIRYGNPASGGASLRATGTMAAPTLDGQLIGCQQN
                     DVVLFHQTPSSPNCSLARLESLRADRTINSLSELNTVPTASPVTILNGADWVRFSCLG
                     AWNQYEYSINAALELTRTGGTPGANPFPNSAAVPIVGDIVALQAQYGITTTIDPTATT
                     ATAAAYLNRVDQWVDATGTFGPAMTLMDRNRIRAIRIAIVARDGILQKGAVSQACDGV
                     ATGLSKVCIWQSDATPASVDLTNLPNWQRYRYRSFEVTIPLRNIIWNRDAL"
     gene            12332..13027
                     /locus_tag="VNZ84_06850"
     CDS             12332..13027
                     /locus_tag="VNZ84_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66714.1"
                     /translation="MRYSKLRPEFLPRQHDGQTGLVLFFALLALAVMSIAAVALIRSV
                     DNNGLLSGNIVFRQSASMTSNVVLEGIAENIAKNITLADSLTHHPSQGYYANCSQFDT
                     QPDALVCDGNQLATMTWDDSNSSLAPNQTDGNDVIRNGVDRQGNEIRYVVERMCNYSN
                     AELAAGTSASDATRCMMASSPSNGETCSHNVNNLELFKRCIAASDSPLYRITLRIAGP
                     KNTLTFMQSFVSN"
     gene            13339..>15852
                     /locus_tag="VNZ84_06855"
     CDS             13339..>15852
                     /locus_tag="VNZ84_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66715.1"
                     /translation="MGNQTSFTAVKADVTETGAAKGGTVNLVSSADYFAYVPGEYCTT
                     AALTSCIKSSQSTTSNTFPAEIRWCNSTSAAAAASLTGSMACQGINDQNTYKYLRQPR
                     AAYTLQITGVSGSVSVTSIKVNNIELLSGTVSASSNAGLANAITASASNSNEGICDSG
                     ATSVLTATSSTSTARTYRKCQVGQTNITSNSGGVIKFTTIAGAPLNLSTPIQVTYSGT
                     ATIKGTWDKATTDVPGSLVYVKIATPLDSYPLPGSTAKSPDRTDCAGSTCTYQEEMTN
                     YANWYTYYRTRLQAMKTAASLAFSNMDSRYRIGFMRICDNPNTMADTFLPVAPFDSAQ
                     KSTWYKSLYAATAVCGYTPIRLALSQSGRYFAGNKKLNAVDPMQYACQSNFTLLTTDG
                     YWNDTTSPTQVNGSTLIGNQDGGNTPRPLYDSSNVSNTLADTAKYFYDTDIRDGGLSN
                     CTGALGSSVCGTANDYPKQNMVTLTLGLGIDGTLVYTEDYKTQVVGDYNDLKVGNKNW
                     PNPIPTENGTRIDDLWHAAVNAGGTYYSAKNPKLLRESLAKGLSEIKSIQGASAAAAA
                     SSLAPTNGDNFQYVASYQSVKWTGNLEARTVNTSTLQTSETAVWCVENVAAALCSNPA
                     VSYTGPDGNFYCKTTNSDQATCASANGYLGSDLSFSEASSCYVPAAKSCTGTLRGQVS
                     TGRNIYFNNGNALTLFSAASLPTTLAQSLSDGYLALSQIQALPATDPKRTDAAKVNKL
                     VDYLKGSKTYEDSSVNAVPDNRLFRERQSVLGDISQSRPAYFKTSNAGYLDSGYKAFK
                     DANSNRKAAVYVGANDGMLHAFNAADGKELWAFIPTPVFA"
BASE COUNT         3872 a         3726 c         4228 g         4026 t
ORIGIN      
        1 tcataagcca gcgcatccag cccggtctct tctttcactt cgcggatggc ggcgtcatag
       61 ggtgtttcac cagcttcagt gctgcccgtc accgattgcc agaacccagc tttgtcagca
      121 cgctccagta gtaacacctg cagatcgggc gtgtggatca aaatgagtgc agagacggga
      181 atcttgtatg cttgcgtcat gcaaatattg tctataaaga ttgccatatt gtctacgaca
      241 gcctttcatt taatcagctt ggcaacatgg tggttgtgaa agattgtggt aagtaactta
      301 cagtattatc atcaaaaaat aatgcataga gaaacaggga atgattcaat gttgaggcgt
      361 cagcgaggct gggcatgcgg gatgctcatg gtatatgcgc aagccatctc tgccggggaa
      421 gcgacctcgg ccagaggcca gttcgttcct cctgagccag gtgccgttca atcttcattc
      481 aatcaggcct tgcttggcca gttgacttta cgccaggcag agcagttgtt tgcaagcggt
      541 aaccgtgaat tgctggctgc caagcgcgcg gtagaaggtg ctgaggcgaa taccctgatt
      601 gcgggccagc gcccgaatcc tgtgttgtcg ttaggacttt ccagtgtcaa tgtcaatcgt
      661 ggccagggga acatgaacca aaatggcgcc aatggcgttt gggacaaaac ctataactcg
      721 gcccttcaag tcagccagtt gtatgagcgt gggaataaac gtgagttacg tacctcggtc
      781 gccgagagtg ccgtcaaagc ttacgggttt gatttgaaag aaacctatcg ccagcaagca
      841 ttggcgatgg ccatggcgta ttatgacctc aaactggcgc aggaaacctt gctgatccag
      901 caggccaatg tcgcctcata tgacaatacc ctgcaggctg cacaattgcg gcttaaagca
      961 ggagatgtgg cttcggcaga tgtggcgcgt attcgtgtag atgctttacg tgccagcaac
     1021 gacttgcgcc aggcagaagc gaccttgcaa aaaacacagg ccacgttagc gtatttgctg
     1081 ggtaaagatc aggatgcctc caaactgtat gcttcagacc cttggcccgg tttgacttca
     1141 gaccaactga cgggtgccga gagtgcctgg ttgccacagc gctcagatgt acaagctgcc
     1201 gaggcgcgca tggagcaggc gcaacaggca agaaagctgg cggaatcgct caagaccagg
     1261 gatgtaacct ggtcgctggc ttaccaacac ttcccgggcc aggagcctgg ttccgcgccg
     1321 gatacagtgg gggcctctgt cagcattccc ttgtttacca actaccagta tgaaggcgaa
     1381 gcggctcgcg ccgaggtcga ttacaccagt gccatggaag ccaaggagca ggtcaaggcg
     1441 gccgcagtgg cggaaatgcg tcgcgccaag gcagatttac aggccgcggt agataaaaac
     1501 cgccggtttg accaaagtat tttaagcgaa gcgcaaaagg cggcagaggc ggctgagttt
     1561 gcttatcagc atggcgcaat gagtgtcatg gacctgttgg atgcaaggcg tattttacgg
     1621 gctttgcaac tggagtctgc cagtgtcaaa gcagattatg ccaaatcact ttctgcttgg
     1681 ctggcggcaa cccgccaggc ctttactaac caagactaac gcctatgacc atgccaatat
     1741 ttaatcatcc cctgacattt ctagcctcta ccaagagcag aattgcattg gtactactga
     1801 ccttgttgac aggacaggct tatgcggcag acaaaaccgt aggtaacgca caaaatcagg
     1861 ccgccaatga aattgtgctg acggctggtt cgccacaatt gaccagttta agggtggaac
     1921 cggtaaaaga aattccggtg ccggtcactg aacccttaaa tggcaagatt gtatttgatg
     1981 aaaaccgcac cgcccgtatt ttttcaccag ttgctggccg tgcactaagt atcaaagccg
     2041 aggtgggtga cgcggtaaaa gttggacaga ctttattggt gatggattct cccgatgtcg
     2101 gtggggcggt ggcggatgta cgcaaatcac aagcagactt gcaacttaag cagcaagcat
     2161 tggagcgtag ccggatgctg gtggatcacg gtgtattggc gaaaaaggaa gtggaagtcg
     2221 cgcaggctga ttgggctagc tcacgtgcag agtctgaacg tgctaccgcc cgcttaaaga
     2281 acctgggagt ttcgacagca gccaatgata actacgcagt gcgctcaccg attgccggta
     2341 tggtggtaga tcgcaagatc aatcctggca gcgaagtgcg tccagatgca gccgatccgc
     2401 tgtttattgt gacagaccct aactacttgt gggccagtat tgatttaccc gagcgagatt
     2461 taagcaggat atcgcagggg cagaagctca gcatacaagt agatgcctat cctgatgaag
     2521 tatttagcgg tgaagtgaaa accataggcg tcatggtgga cccgacgact cgacgcattc
     2581 cggtacggtg tatggtagaa agcaagggca agctcaaacc agaaatgtac gcgcggatta
     2641 cgccgctgga tagcagccag caccgcgtga tccgcttgcc aaatagcgca ttgattaccg
     2701 aagggctgta tagctacgtg tttgttgaag agcgcccctt gcattttaaa aaacgcaaag
     2761 tcacgctgga tttgcagcgc cgcgaatatg ccaccgtgaa agaggggttg aagctgggag
     2821 agcgcgtggt agtcggcggg gccatcctgc tgaattcgga tttgtccgtc agtaaataaa
     2881 gcacatttct tactggctag cttcgaaagt cgatcatgtt aaaaggctta atcacgtttt
     2941 cattacaaca gcgggtattc ctgctgattg gcgcagttgt gctcactatt tttggttggg
     3001 cagcggtgca aaacttgccg attgaggctt tccccgacgt cgaagatgtg catgtgatta
     3061 ttgtgacgca gtcgcctggc atggcacctg aagaggtcga gcgcgtcgtc tcattgccca
     3121 tagagcacgg catgagtggt gtgccaggac tgtcacaaat ccgttcggtg tccattacag
     3181 ggttgtccgt tgtgacatta acatttgccg agcgcacgga ggattatttt gcccggcagc
     3241 aggtgctaga gcgtttgcaa ggcgtgagtc tgccttcagg cttgcagccc acgctggcac
     3301 cattgaccac tgcggtcggt gaggtctatc gctatctggt tgaagccccc caagacatgc
     3361 cgcaacgaga ggtgcgtgca atccaggact ggaagattat cccgcaattg cgcatggtgc
     3421 cgggtgtggc agatatcacc agttttggcg gcacggtcaa ggaatatcaa atcaagctgg
     3481 atccttaccg tctgcggaaa tttggtatca gtatcgatca ggtgaaccag gcggtcacga
     3541 ataatagtag taatgtcggc ggtggcctca tgcatagagg cgatgagtcc ttggttgtgc
     3601 gtgggattgg cttgtttcac aacctcgacg atattgcaca ggcagtcgtg gatacgaggg
     3661 atggcaagcc tatcctggtc agtgaccttg ggcaggtcgt gatcggtgac cggcctcgct
     3721 cgggcattgt gtcgtttaac cagcaagatg acattattga aggcattgtt ctcatgacca
     3781 aagggcaaaa tgcggccaaa gtcattgaag ccctgcgcca aaaggttgag gtggtcaatg
     3841 aatccttgcc tgccggcgtc aaaattgtcc cattctatga caggactggc ctggtacatc
     3901 acactgtgca taccgtcgca gaaaacctga ttgtgggcgc gctgttggtc gtgaccattc
     3961 tggtggtatt cctgcgtaac tggcgcgccg cactgattgt agcgtcagtg attccgctgt
     4021 ctttgctatt ttcttttatt atcatggatt tacgtggtgt ctcagccaac ctgatctcac
     4081 tcggcgcagt agattttggc attattatag atagcgcggt cgtattggtt gaagccctca
     4141 tggtcaggct ggcaatcgcg gaggacgatc acaatcccac ccataacggc cttgcctggc
     4201 gcatgcaaac cttgaaaaat accgccattg atatgggcca tcccatcttg ttttccaagg
     4261 ccattatcat caccgcgttc ctgccaattt ttaccttcca gcgggtagag gggaaaatct
     4321 tctcgcccat ggcgtttacg ctgagctttg cgctgttagg cgccatcata ttgactttga
     4381 cgctggtgcc agccctgttg tcgtacacga tgaaccggga agatatggcc gaaaaacata
     4441 gcgtctggat gcacagactt cagcaaggtt atcgcaatat tttgcttaaa ggcagtcaat
     4501 ccagaatgct tgttgtgctg gcatcggcgg ctgtgctggt ggtgtctgtg gtatcggcgc
     4561 ccatgttggg ctccgagttc ttgcccaagc tggatgaagg taatatctgg ttgaccgtgg
     4621 atctgccacc atcggccagc ctggagaaaa ccaaggaggt cgaacgccat atcagaaaaa
     4681 tcctcaatag ctatcaggag gtcaggctgg tggtagcgca tgtggggcgg ccagacgatg
     4741 gtaccgatcc caaagggcct taccatatgg aaatcttggc agacctcaag ccaaagagcg
     4801 aatggacgtt tgctgataaa gaagccctga tcaaggatat gtcgcgccgg attcacgaga
     4861 ttcctggtgt gccaaccaac ttttcgcaag taattcaaga cagtgtggag gaagcattgt
     4921 ccggagtgaa aggtgaaatt gccgtaaaga tatttggtcc ggatctggcc attctggagc
     4981 aaaaggcgac agaagtggta gatgccatga ataccgtgcg tggcgccacc gatgtggctg
     5041 cagtgcgtat ttccggtaac tctgaactcg atatcacttt aaatcgtagt cagttgtctc
     5101 gctatgggct gatggtgggt gatgtcaaca ataccgtaca gaccgctttg gcgggtaatg
     5161 ctgccaatac tttttatgat ggtgaccgcc attttgatgt aactttgcgt ctggacaggc
     5221 cgttccgcga ctcggtggat gatattgatg aattgccgat tgccttgcct aatggagcgg
     5281 gtagcattcc cttaagtgag gtggccgaag tgagcatcaa acagggggcc tcaagggtga
     5341 gccgggaggc aggtgcacgt attgtctcta ttaaagccaa tattaccgat cgggatcaag
     5401 gcagttttgt gaaagaggcg atgcaaaccg tgaaggcaaa ggtgaagctg ccgccaggct
     5461 atgccatgac ctggggtggc cagtttgaaa accagcaacg ggccatgaaa cgcttaatgg
     5521 tcattgtgcc actgtctgtg ctgctaatat ttgtgctgtt attctgggcg tttaaatcgg
     5581 tgaagaatgc tttgttagtg ttgttgatga ttccatttac actggtgggt ggactggccg
     5641 ggctggcgat tgctggtttg catttgtccg tgtctgctgc cgttggtttt attgcattgg
     5701 cgggtatctc ggtgcaaaac ggcgtgatta tggtcgaaca gatcaaaaaa ttgatgcgtg
     5761 agggccaaag cgtgacggag tcggtgattg aaggggcggt ggcaaggttg aggccaattt
     5821 taatgacggc gttgatggca gggttgggct taatgccggc cgcgttatcc catggtatcg
     5881 gttcagaaac gcaacggcct tttgcagtgg tgattgtggg tggcctgatt actgccacgc
     5941 tgttcacttt gctgttactg ccgctgttgt tcccgctatt ttctgatgtg cgccgccgta
     6001 aatcctgact gggctggttt aagaccacca atgtggtggt ttatttcagc cgatggtgag
     6061 cgatgcttag ataaggtaaa taattaattt tataaaaatc aatgggttat ggttttctgg
     6121 gagttggcac gttgtatgca tttaatggag tagtcactca acctgcagct gggcgaccgt
     6181 gcaatctcca aaatgtaagc gtgaaataat tgcatataac cacaataaca agcatttttc
     6241 cccgattatc ctttcccgag gatattgctt gccccaagct ctcgagcttg ggtttttttt
     6301 gccaaagaac cggttagcct ggtgtgcgct tttcggcgtt gaccagattt tttgggagct
     6361 ggaacaccac attttcaacc acgccttgca gctcttcaac ttgtgcagcg cccatgtttt
     6421 gcagcgccgc aatgacctct ttcactaaca cttcaggcgc agaggcgccc gccgaaatgc
     6481 caattttttg tttgccttgt aaccactcgg ctttcaagtg gctggcattg tctaccatgt
     6541 aggcttccac gccttgattt tttgccactt cacgcaggcg gttggagttt gagctgtttg
     6601 gtgagccaac aatgatgacc aggtcgcaat ccttggccat gattttgacc gcatcctggc
     6661 ggttttgtgt ggcgtagcaa atgtcatcac ttttgggcgc tttaatgttt ggaaaacgcg
     6721 ctttcaaggc attaatgacc tgggtggcgt cgtctaccga aagagtggtt tgtgtgacgt
     6781 aggccagttt ttccgggtct gcgacttgca aggccgtgac atcttccggg gtttccacca
     6841 gatacatgcc gccagcttcc gattgaccca tggtgccttc tacctcgggg tggcctttat
     6901 gcccaatcat gataatctcg aggccatcct tgcgcatctt ggcgacttca atatgtactt
     6961 tggtcaccag tgggcaagtg gcatcaaacg cagtcagtcc gcgagactct gcctctgcgc
     7021 gtacggcttt agagacgcca tgcgcactga aaatcacgat gctgcctgcc ggaatttttt
     7081 ccagctcatc gacaaaaata gcgcctttat cacgtaactg ccccaccacg aacttgttat
     7141 ggacaacttc gttacggaca tagatgggcg cgccgaattt ttccagcgcc tgttcgacaa
     7201 taataatggc acgatccacg ccagcacaaa atccgcgcgg gttggccagg actatattag
     7261 gtgtcacttc tgtttgcata atgaattcca tcataaggca gctgcatttt gcgcccgcaa
     7321 taccaaggtt ggccacaagg tctggcaggc atcaggtata ataggccttt tgtcgctagc
     7381 gataacgtct attgttatga aaaacactgc tactttgctg attacctgcc ccgataccaa
     7441 agggattgtg gcggcgattg cgcaattttt gtatgagcac aatgccaata ttttacacgc
     7501 agaccagcat caggatgcgg aaaataatct gtttttgatg cgcgtagagt gggatctgac
     7561 caatttcaac gtggttgtgg ccgattttga acaggcattt ggcccggtgg cgcagcgttt
     7621 tgatatgact tggcagctca aactctctga gcaaaaaaca cggatggcga tcatggtctc
     7681 gcaatatgac cattgtctgg tcgacttgct gcatcggcat catagtggtg aactggcctg
     7741 cgagattccg ctgattatca gtaatcaccg tgataccgaa aaactggcgc agttctatgg
     7801 catcccgttc cattatctgc ccatgaccaa ggaaaccaag gctgaggtag aggcacagca
     7861 gtttgcccta tttgaccaac acaagattga cctgattgtg ttggcgcgct acatgcagat
     7921 tttatcgcca gattttgtcg cacgttatcc acagcgaatt atcaacattc accactcatt
     7981 cctgccggcc tttatcggtg cgcgtccgta ccatcgcgcc tttgagcgcg gcgtgaagct
     8041 gattggtgcg accagtcact atgtgaccga agtgttggac gaaggcccga ttatcgagca
     8101 ggatctggcg cgtatttctc accgtgacca agtcgaggac ttgatccaga aaggtcgcga
     8161 cttggagcgc gtcgtgttat ctaaagcggt acgctggcat attgagcata gaattttgct
     8221 ctatgcgaac aagacggtga tctttgatta aagggaatgc tccatccata aaaaactgaa
     8281 gcttggcggc ttcggttttt tatttggtcc tgaatgttat ggtagcgtgc gctccaattt
     8341 aaacaggtca gcaatttgtt cacgttcgcg gatcacatgc gcctggtcac catctaccaa
     8401 tatctctgct gcacgggcgc gcgtgttgta attgctggcc atgctcatac cataagcgcc
     8461 tgcagaatga atcgccaggt aatcgccttc ttcgatcgca agtgtacggt catggcctag
     8521 aaagtcccca ctttcacaga ctgggccaac aatttcatag atttgcgctg gtgcagcgga
     8581 aggcgcaatg gtggtgatat tgtggaaggc atcatacaaa gccgggcgca tgaggtcatt
     8641 catggccgca tcgacaatcg cgaaattttt cgattcgccc ggtttgagat attcgacctt
     8701 ggtcagcaat aaacctgcat taccgaccag tgcgcggcct ggttcaaata gcactttcac
     8761 atttctgcct gccagttttt tcaaaatggc agcggtgtag ctagcaaagt caggcggggt
     8821 ttcgtcgctg taagtaatac ctacaccgcc accgacatca atatgctgga tatgaatgcc
     8881 tttggcggcc aatgcatcga ccaggccaag cactttatct agggcgtcca ggaaaggaga
     8941 cagctcggta atttgtgagc cgatatgaca atctacgccg tgtacttcga tattgggtag
     9001 ttgcgcggct ttttcataca agcccagggc atcctcaaag gcgacgccaa atttattatt
     9061 tttcaggcca gtggaaatgt aaggatgggt ttttgcatcc acattcgggt tgacgcgcaa
     9121 tgagaccggc gcttttttgc ccaggtctgc ggccacttgc tggatacggt ccagctcgtt
     9181 taccgattcc acgttaaagc aaagaatgcc cgcctcaagt gccgctttga tttctgcgtg
     9241 tgattttcct acaccggaaa acaccacttt cttcgggtcg ccacctgcgg ccaatactcg
     9301 ggctagttca ccgccagaca caatatcaaa ccctgcgccc aaacgagcaa acagattcag
     9361 gattgccaga ctgggattgg ctttgactgc gaagcaaatc aaatggtcgt gtccgaccag
     9421 gccagcttgg aagcgctcaa aagcctgggt taaggcggcg cgtgaataga cgtaagttgg
     9481 cgtttgatgc gtttgtgcaa tgtcacgcaa ggctacattt tcggcatgta gcaggccttg
     9541 ttggattgaa aaagcggtca cgatgaagac ttatcttgtt ggggcgtttg agggtattgg
     9601 cgctcaggaa tatacaggtc ccctttgaga ccacaggcac tcaattgtgc caataacagg
     9661 gcaattgcca gtaatctcgt ccaggaggaa agcatgttca ccatccaata taaatagact
     9721 gacattttac cattaaaccg ctagcggctc gaaggcttgg gcatcaaccg cttgtcggcc
     9781 aattcaaacc attagtcggc tcgggctgga catgacatgt gtctgcttac atgataagcc
     9841 tatcaaaaaa ggggtttgat aatgaagcac tcaaatcaat tcggatttag cctgatagaa
     9901 gtcatggtcg tggtggccat tatgggcatc ctagccagta ttgcgatacc gggctatcag
     9961 gattacatca agagcggcaa tgctgcagaa gcgcctgcca atctggccaa ttgccgtgtg
    10021 caggccgaac agttttatca ggataacttc acttatgtag gatttgcctg tgtcccaagt
    10081 gatgccaaat tttttgatta cagcattgat aaccagtctg cgaccactta taccttaaaa
    10141 gcgaccggta aatctggcca gaacatgggt aatttttcgt ttacggtgaa tcaggataat
    10201 gccaaaacct ccacgtttga tggtacgacg ggaagtacct gctggttaac ctcctcttct
    10261 ggtagctgtt aatcatgcgg caaaaaggat tcactctggt agagctggtg gtcaccatgg
    10321 tagtcctggt ggcggtttct agcattgcga ttcctgcttt tcaaactgcg attggtaatg
    10381 cacaaatccg taccgttgct gaatctatcc gcgatggtct gcagcaggcc agggtagagg
    10441 ccatcaaacg taatgcacgc gttaaattca cattgcagac caatagtgca tggcaagtgg
    10501 gttgcgtgac acaaacaact gcttgcccta cattgattac ccagaaaagt gcgagtgaag
    10561 ggtcttcaag caataccaca gtggctgccg acaattacac ggcagtgttt agcagctttg
    10621 gcacccgtga tccggctacc ccggcgggtc tatccgtggt caacatcact aatggccagg
    10681 taaaggcgga agagcgcaag gcgctaaggg tcatgctggc ggctgggggc aatgtcaaag
    10741 tctgtgaccc atccgttacc acggtaggag atccaagagc atgctaataa gtatttctcg
    10801 gcgtcaacag atgactttta cttcgatgca acaaggctcg gtgctgattg aggccatggt
    10861 tgcgctggtg attttcagca tgggagtgct ggcacttgtg gggttgcaaa gtgccatgat
    10921 caagaatagc agcgataatc gctatcgcgc cgaagcacag ctgattgcac aaacgcatat
    10981 tgctaacatg atggcttatg gtggagatgc ggccaactat attacccagg tggataagga
    11041 caaaatcaag tcgcagttgc caaatggcac actgacattt tcagcattga ccaataccat
    11101 ggtgacggtc accgtgggtt ggcaggtgcc tggcggcaac ccgcatcagg tcaatgcttc
    11161 cagttatttg tttgatgtga tgccatgatg aagcagtcca cctggacatt ggcacgcttg
    11221 catcgccctc gtttccaggc cggattgagc ctggtcgaat tgttgatcgg cctgttaatc
    11281 ggtttagtcg caacattggc gatcagtaac ctgttcagcg gttttgaggc gcgcaaacgc
    11341 atgattgcgg gtggcgctga tgcacaatcc agtggtgtgc tggccatgta ttacatgcag
    11401 cgtgatgcgc aaaacgcggg ctatggcttg ccgctgtata acagtagtga cccatcgcct
    11461 ttgttatgcc ctattgatac ctcaatcaat cagggtggtg tggtgattaa tttgtcgcca
    11521 gtgatcattg ttgatggcgg cacgggtaat gatattgtgc gtatccgcta tggcaatcct
    11581 gctagcggag gcgcttctct gcgagcaact ggtacgatgg cagctccgac actggatggg
    11641 caactgattg gatgccagca aaatgatgtg gtgttgttcc atcaaacccc aagcagcccc
    11701 aactgcagtc tcgctcgctt agagtcacta cgcgctgatc gtacgatcaa ttctttatct
    11761 gaattgaaca ctgtcccaac ggccagccca gtcaccattc tgaatggtgc cgactgggtg
    11821 cgcttttctt gcttgggtgc atggaatcaa tatgagtaca gtatcaatgc tgcactggag
    11881 cttacccgca cgggaggaac gccaggagct aatccgtttc ccaatagtgc ggctgtaccg
    11941 attgtaggcg atattgttgc attacaggcg caatatggaa ttaccacaac aattgatcca
    12001 acagcgacaa cagcgactgc tgcagcgtat ttgaaccgtg tagaccaatg ggtagatgcc
    12061 actgggacgt ttggtccggc catgacgctt atggaccgaa atcgtatccg ggcaattcgt
    12121 attgccattg ttgccagaga tggaatcctg caaaaaggtg cagtcagtca ggcctgtgat
    12181 ggcgttgcaa caggattaag taaagtgtgc atctggcagt cggatgccac acctgcaagc
    12241 gtggacttaa caaacttgcc aaactggcaa cgctatcgtt atcgcagctt tgaagtcact
    12301 attccacttc gaaatattat ttggaaccgc gatgcgctat agcaaattac gtccagaatt
    12361 tctgccgcgg caacatgatg gccaaaccgg tctggtgttg ttttttgcgc tattggcact
    12421 tgcggtgatg tcgattgccg ccgtggcctt gatacgcagt gtggataaca atggattgct
    12481 atcaggcaac attgtattca ggcagtctgc cagtatgact tccaatgtgg tgttggaagg
    12541 tatcgctgaa aatattgcta aaaatataac gcttgccgat agtttgacgc accacccctc
    12601 acaagggtat tacgcgaatt gcagccagtt tgatacacag cctgatgccc tggtttgcga
    12661 tggcaaccaa ctagccacga tgacctggga tgattcaaat agtagtctgg cacccaatca
    12721 aacggatggt aatgatgtca ttcgtaatgg tgttgatagg caaggcaatg aaattcggta
    12781 tgtggtagag cgtatgtgta attacagcaa tgcggagttg gcggcgggca cctcagccag
    12841 tgatgctacg cggtgcatga tggccagctc gccgagcaac ggtgagacat gttcacataa
    12901 tgttaataat cttgagttgt ttaaacgctg cattgcggct tctgactcac ctctataccg
    12961 tatcacgctg cgtattgccg gtcccaaaaa cacactgacc tttatgcagt cttttgtctc
    13021 caattaaatg caggtggcat gatgacaaac actcacaatt ggctcacgaa actagtttgt
    13081 ttggctttgc tgttacccgg ggtattgcga gccgagttct tggatttggc gaccatgccg
    13141 ttacagacct ctgggtctgg ggacgtgaag cccaatttaa tgtttatctt ggatgattca
    13201 ggtagtatgg ggcgcacgta taccccggat tggtctagca gcaacttcgg ttggttactc
    13261 gataatgctt cgtataacac gcagtattac aaccctagtg tgacatatct cccggctgtg
    13321 acatcagcta atgtttctat gggtaatcaa acctctttta cggcagttaa agcagatgtg
    13381 actgagaccg gggcggctaa aggcggcaca gtcaatttag tcagtagtgc agattacttt
    13441 gcctatgtgc ctggggagta ttgcaccaca gcggcgttga ccagttgtat caaaagtagt
    13501 cagtccacca ccagtaatac ctttccggcc gagattcggt ggtgcaattc aacttcagcg
    13561 gccgcagccg catcgttgac gggatctatg gcatgtcagg gtattaatga tcagaacact
    13621 tataagtatt taaggcagcc ccgtgcagct tatacgctac aaattacggg tgtgagtggt
    13681 tcggtttctg ttacaagcat caaggtcaac aatatagagt tattgtccgg cacggtgtcg
    13741 gctagtagta atgctggctt ggcaaacgcg attacagcgt cggcctctaa tagcaatgaa
    13801 gggatatgtg actctggtgc tacatcggtg ctaaccgcaa catcatcaac ttccacagca
    13861 agaacctatc gtaaatgcca ggtgggtcaa accaacatca ccagcaactc tggtggagtg
    13921 attaagttca ctactattgc tggagcccca cttaatctca gtacgccgat acaagtgacc
    13981 tactctggga cggctaccat caaggggaca tgggacaaag ccacgactga tgtacctgga
    14041 agtcttgtgt acgtcaaaat tgctactcct ttagatagtt atcctctgcc tgggtcgact
    14101 gcgaaaagtc cggaccgtac ggattgcgca ggcagtacat gtacctacca agaagaaatg
    14161 acgaattacg ctaattggta cacgtattac agaacgagat tgcaggcgat gaagacggcc
    14221 gccagtcttg catttagcaa tatggactct cggtatcgca ttgggtttat gagaatctgt
    14281 gacaacccca acacaatggc ggacactttt ttacctgtag cgccatttga tagcgctcaa
    14341 aaatccactt ggtacaagag tttatatgca gcaactgcgg tttgtggata tacacccatt
    14401 aggttggcgc tctctcaatc agggcgttat ttcgcaggga ataagaaact gaatgctgtg
    14461 gatccgatgc aatatgcctg tcagtctaac ttcaccttgc tgacaacaga tggttactgg
    14521 aacgatacaa cgagtccgac tcaggtgaat ggcagtaccc tcattggtaa tcaggatggc
    14581 ggcaacacgc cccgcccttt gtatgacagt agtaatgtgt caaacaccct ggccgacacc
    14641 gcaaaatatt tttatgacac agatatccgc gatggtggac tatcaaattg tactggcgct
    14701 ttaggcagct cggtatgtgg cacggcaaac gactacccaa agcagaatat ggtgacactc
    14761 accttaggcc taggtattga tggtactttg gtatacaccg aagactataa aactcaagtg
    14821 gttggggatt acaacgattt gaaagtgggt aacaaaaact ggcccaaccc gatcccgact
    14881 gagaatggga ctcgaattga cgatttgtgg cacgcagcag tgaatgcagg tggtacttac
    14941 tacagtgcaa aaaaccctaa attgctacgc gaatctttag ccaagggctt gtcagagatc
    15001 aagtctatac aaggtgcttc cgcggctgct gctgccagta gtcttgcgcc gaccaatggc
    15061 gataactttc aatatgtggc aagttatcag tctgtgaagt ggacaggtaa ccttgaggca
    15121 cgtacagtga atacgagcac cttgcagact tctgagaccg ctgtctggtg cgtggagaat
    15181 gtggccgccg cactttgtag caacccggca gtgtcatata ccggaccaga tggaaacttt
    15241 tattgtaaaa cgactaactc agaccaggcg acttgtgcca gtgccaatgg ttatttaggg
    15301 agtgatctga gttttagtga ggctagctca tgctatgtac cagcggctaa gagctgtacc
    15361 ggcactttgc gtggacaagt tagcacgggc agaaacatct actttaataa cggcaatgcc
    15421 ttaacgttat tttcggctgc cagtttgcca acaacgcttg ctcaatctct ttcagatggg
    15481 tatctggctt taagtcagat tcaagcgtta ccggcgacag atcctaaacg gacagatgct
    15541 gcgaaagtaa acaagcttgt agattatctc aaaggaagta aaacctatga ggatagcagc
    15601 gtcaatgcag tgcctgataa tcgactattt cgcgagcggc aatcagtgct aggggatatt
    15661 tctcagtccc gtccagctta ttttaaaacg tctaatgccg gttatttaga tagcggctat
    15721 aaagctttca aagacgctaa ttcaaatcgt aaagcggctg tatatgttgg cgcaaatgat
    15781 ggcatgctgc atgcatttaa tgcggctgat ggcaaagaat tgtgggcgtt tattcctacg
    15841 ccagtgtttg ct
//
LOCUS       DATMSK010000207        32808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_438252,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000207 DATMSK010000000
VERSION     DATMSK010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 32808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32808
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438252"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VNZ84_06860"
     CDS             complement(<1..446)
                     /locus_tag="VNZ84_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWU66716.1"
                     /translation="MASISPPVCDFGWKAPTFNLPDVDGKLVNLNDGMGPNGLLVMFI
                     CNHCPYVKAILPRLIADVKALQALGVNTVAIMSNDPSDYPEDSPENMKKLSDELALPF
                     PYLLDETQQVAKAYDAVCTPDFFGFNNNFELQYRGRFDESRKETAP"
     gene            560..1294
                     /locus_tag="VNZ84_06865"
     CDS             560..1294
                     /locus_tag="VNZ84_06865"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HWU66717.1"
                     /translation="MSNLRFHSPTALAVGQQAPLSENAMAHAVRVMRLKVGDTLTLFC
                     GDGFDYQCALTSIEKKSAQVEVLSHIEVSNESPLTIRLLQGISSGDRMDYTIQKAVEL
                     GVTEIYPLSTERCLTKLSGDRAEKRIEHWQGVAIAACEQSGRAIVPTVHAPLTLAQWL
                     SQHATPDCLNLLLNPVGAKRLAALSKPANATHLLIGPEGGLSPAEIALATQHHFQSIV
                     LGPRILRTETAALTAIASMQSLWGDF"
     gene            1301..2230
                     /locus_tag="VNZ84_06870"
     CDS             1301..2230
                     /locus_tag="VNZ84_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HWU66718.1"
                     /translation="MFQVMLQMALLIAAGMLWQRLAPDHIPAISHRRALTDLVFYILL
                     PALVIDVLWQAPLDADTFSIQLTAISRLSVAALLMWLCLQVLKRFFDVPRAQQGALML
                     AATFPNATYLGLPVTSEVLGDWAHEMVLKFDLFACTPILLSLGMLLGQHYGEAATKSN
                     PFKELIRVPPLWALVIACALNLLHVPQPEMIAHALHTLAGGVIPLMLVALGMSIRWDT
                     FKLRLIPLLLPVCVIGLLIAPLAARYAAIALGLHGNQLTACILLAAMPTMVFGIVICE
                     RYRLDGALYAAAVFMSTLLSIASLSLWFVILQP"
     gene            2401..3573
                     /gene="cfa"
                     /locus_tag="VNZ84_06875"
     CDS             2401..3573
                     /gene="cfa"
                     /locus_tag="VNZ84_06875"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HWU66719.1"
                     /translation="MPKTTTNQYIAPQPTPPATLVSLLAETGVTINGNAPWDIQVYEE
                     SVYRDVLTKGTLGLGQAFIEGRWDCKRLDEFFHRVMRADIDEKMGGMAKLKLLGEIIR
                     HSFFNLQTPDRAYQVAQQHYDIGNDVFEAMLDSSMSYSCAYWEHASTLEEAQFNKLDM
                     ICRKLDLQPGDRVLEIGCGWGGLAKHMAKYYGVQVVGITVSVEQQALAKERCAGLAVD
                     IQLKDYRELHGQYDKIVSVGMFEHVGPKNYEVYFATAKRLLKDNGLFLLHTIGNYKTA
                     LHTDAWIDRYIFPNGKLPSASEITNAIEQEFLIEDWHNFGPDYDRTLMAWWDKFNRAW
                     PELQFKYDESFYRMWKYYLMCCAGYFRSRQGQLWQLVLSKRQSNRIYRSVRPLPAS"
     gene            3835..5403
                     /gene="argA"
                     /locus_tag="VNZ84_06880"
     CDS             3835..5403
                     /gene="argA"
                     /locus_tag="VNZ84_06880"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HWU66720.1"
                     /translation="MLGSNIRDLRKSQNLTLQQLAARMGTDASNLSRIERGEIGISEA
                     LLRAAAQALNTTPGRLYDANLTPVSLTNTPPAILSSANSKDFVHWFRSAAPYIHAFGG
                     STFVIAFGGEVVSEQQFIALSHDLNLLASLEVRLVLVHGARPQIEQRLKRDNLTPKLH
                     NGRRITDDAAMEAVKEANGAIRVEIESLLSMGIANSPMAGSDIRVASGNFVTAKPLGV
                     REGVDLQHTGEVRKIDAIGIQKRLDDGELVLLSPMGYSPTGETFNLTLEDVAVSAAIA
                     LDAEKLIFLMDSEGVQNLRGELLREMTSAKAKNLLRNVQESDEPIHYSEDVNYYLPAA
                     VRACEHGVARTHLISRHIDGAIIQELFTRQGIGTMITELPLETMRQANIDDVGGILML
                     IEPLENEGILVRRGRERIEMEINYFYVMEHDNRVIGCAALYPFPQEKMVEFACLAIDP
                     AYRGGGRGDKLFNFCQQQASQKGFKKLFCLTTRTEHWFLERGFTELEIDQLPQEKQKL
                     YNFQRKSKVFIKSL"
     gene            5475..6356
                     /gene="argB"
                     /locus_tag="VNZ84_06885"
     CDS             5475..6356
                     /gene="argB"
                     /locus_tag="VNZ84_06885"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWU66721.1"
                     /translation="MQTSAASQKAQTLSEALPYIKRFFDKTIVIKYGGNAMTDDRLKE
                     CFAQDVVLLKLVGMNPVVVHGGGPQINEMLDKLGKKGEFIQGMRVTDEETMDVVEMVL
                     GGQVNKEIVNLINRHGGKAVGLTGQDGNFIHAHKLLMEDLKDPTKMIDVGQVGEISGI
                     DPSIITFLDSGDFIPVVAPIGVGKDGETYNINADVVAGKLAEILNAEKLILLTNTPGV
                     LDKSGQLLTGLTPKQIDDLVEDGTLSGGMLPKIGSALDAARSGVKSVHIIDGRVEHAL
                     LLEVLTDEGVGTLIKAK"
     gene            6395..7051
                     /locus_tag="VNZ84_06890"
     CDS             6395..7051
                     /locus_tag="VNZ84_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HWU66722.1"
                     /translation="MSRRVWIFDLDDTLHNASAHIFPVMNQCMTHYIMQTLDMEEAAA
                     HQLRQHYWRIYGATLKGLMRHHGVNPHHFLAETHAFLTDDMVQTTRQLRQLLNSLPGR
                     KCVFTNAPRAYAMRVLEVLGIADCFSLVFSVESSRFHAKPSMRGFRMLLRQLRCRAAD
                     CTLFEDSLPALMTAKRLGMRTVWISRRLHKPNFVQYRLPHVLALTHSALKKGQNAFPC
                     "
     gene            7115..7708
                     /gene="slmA"
                     /locus_tag="VNZ84_06895"
     CDS             7115..7708
                     /gene="slmA"
                     /locus_tag="VNZ84_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HWU66723.1"
                     /translation="MATINKEARANRKQQILETLAGMLQNPRGEKITTAALAAKLEVS
                     EAALYRHFANKAKMFEGLIEFIEGALFGVMNKITTDETDGLKQVQLMLQTMLKFAERN
                     PGMSRVLIGDALVNEDESLQARINQLLDRLEASLKQSLRIAEAQGHKVADAGIAANTL
                     ISFVLGRWHAFAKSGFKRKPSDNLEQHVTQLITSCLQ"
     gene            7730..7954
                     /locus_tag="VNZ84_06900"
     CDS             7730..7954
                     /locus_tag="VNZ84_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HWU66724.1"
                     /translation="MENTVFGMNEAQLTEFGMSWGLAGLILFIVFIVGNLAWKSKAGK
                     TGTFALFLALTLGMVGFLAKLLIQHYLDIH"
     gene            8006..8644
                     /locus_tag="VNZ84_06905"
     CDS             8006..8644
                     /locus_tag="VNZ84_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWU66725.1"
                     /translation="MQQILVYSDSLSWGIIPNTRQRLPFDQRWPGVLEIILNQPGSHF
                     RVIEDCLNGRRTVFEDPYKPGRNGLQGLAQRIEIHSPLALVILMLGTNDFQSMHLHQA
                     WHAAQGIASLIHAIRHAPIEPGMPVPPILVVAPPAVTHPQGLIAEKFAGAEEKCKGLA
                     EAYAAVSQSLDCAFFDAGSVVSSSQIDGVHLDAAQHLILGKAIASQVNRLIA"
     gene            8724..9659
                     /locus_tag="VNZ84_06910"
     CDS             8724..9659
                     /locus_tag="VNZ84_06910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWU66726.1"
                     /translation="MDNSPPSDSDTYKTLLESTKAIPWKIDWASKQFTYIGPQIEPLL
                     GWAPESWHSVEDWVARIHEEDRERVFNFCVQQSLQGLDHEADYRALAADGSYVWIRDV
                     VHVLRTPNGEPEALIGFMFDISERKKTEEQLLMMQKQLEEFSFKDGLTGVANRRMFDT
                     VLEREWLEARQHQQPLSLIMIDIDYFKQFNDFYGHLQGDEVLKQVAKTLSHAGVRAKD
                     FFARFGGEEFALILPETNALSASKIAERCRNLVFKEQIPHAKSEVSQILTISVGAGSI
                     TPGPADDLKAFVESVDALMYQAKHDGRNRIVVKSA"
     gene            complement(9691..10911)
                     /locus_tag="VNZ84_06915"
     CDS             complement(9691..10911)
                     /locus_tag="VNZ84_06915"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HWU66727.1"
                     /translation="MQIYQVGGAVRDSLLGLPVKDRDYVVVGATPEQMLAAGYRPVGK
                     DFPVFLHPQTQQEHALARTERKTAKGYKGFSIHAAPEVTLEEDLARRDFTINAIAQAE
                     DGSLIDPFHGQLDIQKKILRHVTDAFSEDPVRILRAGRFLARFTDFTVAPETLTLMRD
                     MVAAGEVDALVPERVWQELAKGLMEKKPSRMFEMLRDCGALQVILPELDRLWGVPQTA
                     TYHPEVDTGVHVMMVIDYAAKQNYSLPVRFAALTHDLGKGTTPAEVLPRHIGHELRSV
                     DLIREVVLRLRVPNDCKDLALVVAKLHGKLHAARQMKASTLLQFLEELDAFRQRTRFE
                     DFLLACECDSRGRLGLEDCALDDARHLSAALHAALTVDAGAIAQACSSPAHIKQSVAE
                     ARIQAIQSALHLHH"
     gene            complement(10930..11892)
                     /locus_tag="VNZ84_06920"
     CDS             complement(10930..11892)
                     /locus_tag="VNZ84_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HWU66728.1"
                     /translation="MMKTVTVLGGSGFVGSSVVARLDQAGYQVKVLTRRREQAKHLIL
                     LPKVQVVECDIHDHAALKAQLQGSDVVINLIGILHQTRDNGFEQMHHQFPRRVAQLCE
                     ELRIPRLLHMSALQASVNAPSEYLRSKAAGDQAVMEFSKKLNVTIFRPSVIFGTRDRF
                     INLFAKLIQWIPVLALAMPQAKFQPIWVEDVAAAMVNAVDEPATYGQTYELGGPAIMT
                     LQQIIEAVMKTIHVQRPIIGLSLKMSLLQGSFMQLLPIKLISRDNVKSMQVDNVCQQP
                     MANELRVVPTDMFAVISGYLVKNNPRGAYDHFRAAAGRVINARR"
     gene            complement(11949..13928)
                     /locus_tag="VNZ84_06925"
     CDS             complement(11949..13928)
                     /locus_tag="VNZ84_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWU66729.1"
                     /translation="MKTRLTSLLIFAACLLAMTKSVFAGDGEVYLQAKQAYQARNLPQ
                     LIEATETLKQRRSPLLPYLQYWQMILTLDQLSYDQVQAFLQENNESLLSQRVRELWLK
                     RLGRVQFWDQFVEMRAQIPMYYSFNDLGNQCYQVQAGIALDDPNAYEDGKKLLLTGKD
                     LPADCQGMMEALQQVGVLDEKLLLTLYRDALFSNKTGLAKVLAKRSSKTDAGLFKQID
                     DMAQNPALALKKGNIQQRSAYQRALYLYAIHRQAKADTSVARASYQKYSGFLDNEEKQ
                     LAQAYIALEAARKHESDALQGFSKVNAVILSSEQWEWYARSALRQQDWERLLKVINEM
                     PAALSDESAWRYWKARALIAKGQIADANQILAKLSQERHFYGWLAAEDLGPVAGEPMA
                     TYQPTDEEVRQFARQPVVKRMEVLFDVEARYEARLEWLYLLEVLDDPTRIVAAQYALL
                     KGWYDLAVLAADKTSRTHNFELRYPTPYRDYLQKASRNRAIDEAWVYGIIRQESRFMH
                     YAKSSVGAGGLMQVMPATAKWIAKKLGLNGYHDGMLHDIDTNVNLGTYYMRYTLDTFN
                     GQEVMATAAYNAGPSRARRWAGSTPLEGAIYAETIPFSETRNYVKKVLANAHMYAPRL
                     GLPMLTLKRRLGTIPARTTAEVESVTTTISISEQE"
     gene            complement(13962..14144)
                     /locus_tag="VNZ84_06930"
     CDS             complement(13962..14144)
                     /locus_tag="VNZ84_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66730.1"
                     /translation="MWWIRILFTVLVVSTLVLVLLGWQTGQARYWRWLKSVWRGGFIV
                     ILLLLLFFFLSRLARI"
     gene            complement(14165..15100)
                     /gene="lpxC"
                     /locus_tag="VNZ84_06935"
     CDS             complement(14165..15100)
                     /gene="lpxC"
                     /locus_tag="VNZ84_06935"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HWU66731.1"
                     /translation="MLKQRTLKQPISATGVGLHSGEKVTLTLKPAPADHGVVFQRIDL
                     PDTPAMRVAPTAVNDTRLSSALEFSGARVATVEHLMSALAGLGVDNVLIELTAAEVPI
                     MDGSSGPFIYLLQTAGLQELDAAKKFVRILKPIEVQEGDKWAKFTPHHGFKAEFTIDF
                     AHPVFEFSGKTVTIDYDSQSYVDEISRARTFGFMHEVEYLRANGLAKGGSLDNAIVLD
                     EYKVLNGGGLRYDDEFAKHKVLDAIGDLYILGYPIIGQFTAFKSGHALNNQLLRALLA
                     DDTAWEYCTFDNDAHAPVGFIKQRDYFSPAPLLPA"
     gene            complement(15187..16347)
                     /gene="ftsZ"
                     /locus_tag="VNZ84_06940"
     CDS             complement(15187..16347)
                     /gene="ftsZ"
                     /locus_tag="VNZ84_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWU66732.1"
                     /translation="MIEIFDKENDGAVIKVIGVGGCGGNAVEHMMTKSVTGVEFICAN
                     TDMQALKKSHAKVVLQIGSDITKGLGAGAKPEIGREAALEDRDSIAETIDGADMLFIT
                     AGMGGGTGTGAAPIIAEVAREMGILTVAVVTKPFAFEGKRAKVAQEGLEELSQYVDSL
                     IVIPNEKLMDVLGEDVTVVEAYRAANDVLHNAVSGIAEIINCPGLVNVDFADVRTVMS
                     EMGMAMMGSAEASGPNRARIAAEQAVASPLLEDVNLANARGVLVNISASGSFKMREYY
                     DVMNTIKEFTAEDATVIVGNVMDESMGDNLRVTMVATGLNGSLGKRQAKPELKVMTTL
                     RDGTTNQPIFSAVEEDEPAVFTSNSRRAQVEAMKNSGIEEYDIPAFLRKQAD"
     gene            complement(16430..17668)
                     /gene="ftsA"
                     /locus_tag="VNZ84_06945"
     CDS             complement(16430..17668)
                     /gene="ftsA"
                     /locus_tag="VNZ84_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWU66733.1"
                     /translation="MSRVREDKNLIVGLDIGTSKIVAIAAEILPEGTLKVIGVGQHAS
                     RGLKKGVVVNIDSTMQAIQRAIEEVELMADCKINTVYSGIAGSHIKSLNSHGMVKIKE
                     SEVSQMDIDRVVETARAIALPADQQILHILTQEFIIDGQEDVREPLGMSGIRLEVRVH
                     IVTGAIAAAQNIVKCIKRCGLEVSDLILQPLASSESVLTDDEKELGVCLVDIGGGTTD
                     LAVFKNGAIRHTAVIPIAGDQITNDIAVALRTPTQSAEEIKVKHGCALRQLADPREVV
                     EVPAVDGREPRQLSCQALTEVIEDRVEEIYEFVQQELRRSGMESMIASGIVITGGAAQ
                     MRGMVELGEEIFHTPVRLGVPRGVEGLSEVVGNPRYATGIGLLLMAKQQVEKQMVGNL
                     QSGSVGRLLERMKSWFTGNF"
     gene            complement(17706..18410)
                     /locus_tag="VNZ84_06950"
     CDS             complement(17706..18410)
                     /locus_tag="VNZ84_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HWU66734.1"
                     /translation="MWDRPTLLNWIANLLFSLAVVMLLYAALFVVIHLPIFPVKQVQV
                     EGNLDHVNHEQIQLIVSKYLKGNFYTLDLQRTRIAFEKLPWARKVTVRRQWPDTIVVQ
                     IEEHQALGRWGGVALVNKHGELFQAASDEQLPTFYGPGDAVKEVAQGYASFAQMLTET
                     PLEIQQLSLSSRRAWEIRTADGRQMMLGREAAKQRLQQFVLAYRQQANIAESDWRYAD
                     LRYPNGFALRMPRPGA"
     gene            complement(18427..19347)
                     /locus_tag="VNZ84_06955"
     CDS             complement(18427..19347)
                     /locus_tag="VNZ84_06955"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWU66735.1"
                     /translation="MSAYQQTFGKVAVLFGGRSGEREVSLKSGSAVLAALQRQGVDAH
                     AFDPATHELSALKAYDRAFIALHGRFGEDGTIQGALELMDIPYTGSGVMASALGMDKW
                     RTKLLWTAAGVTTPNYVLMDDSSHVENVVTALGLPLFVKPANEGSSIGVSKVKQAGDL
                     AAAYTLAKQSDPLVIAEQFVGGGEYTVGILGDTALPIVRIVPKNEYYDYEAKYLRDDT
                     EYLCPCGLSAEQEKQIQAEALQAFKVLGCKGWGRVDFLMDEAGKHYFLEVNTSPGMTD
                     HSLVPMAAKAAGMDFDALVVRILQQTLDKV"
     gene            complement(19344..20249)
                     /gene="murB"
                     /locus_tag="VNZ84_06960"
     CDS             complement(19344..20249)
                     /gene="murB"
                     /locus_tag="VNZ84_06960"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWU66736.1"
                     /translation="MNASGLKTTGLQVLHDEPLARYTSWRVGGPADRLVLADSVEQLQ
                     QFLQQLSASEPLTFIGLGSNLLVRDGGVRGTVVVMHQALQALEMQGERIYADAGVTCA
                     KLARFAASQNRVGAEFMAGIPGTVGGALAMNAGCYGGETWQWVSEVKTINRAGAVQVR
                     ARAEYQPTYRHVQHPAADEWFLGGWFTVPAGDGKASAEQIKQLLAKRLASQPLNMPSA
                     GSTFRNPEGDFAARLIEASGLKGTTIGGAQVSEKHANFIVNLGHASASDIEALIEHVK
                     TTVRTKQGVALVQEVKVIGESVLTA"
     gene            complement(20246..21640)
                     /gene="murC"
                     /locus_tag="VNZ84_06965"
     CDS             complement(20246..21640)
                     /gene="murC"
                     /locus_tag="VNZ84_06965"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWU66737.1"
                     /translation="MKHKVKKIHFIGIGGSGMSGIAEVLLNLGFEVSGSDLASNATTK
                     RLQGFGATVYQGHASEYLSDADVVVVSSAINEENPEIIAARARKVPVIPRAVMLAELM
                     RFKQGIAVAGTHGKTTTTSLIASILAEAEMDPTFVIGGKLEAASANARLGTGEWIVVE
                     ADESDASFLHLTPILAVVTNIDQDHMDTYEHSFDKLKTAFVEFLQQIPFWGMAVMCID
                     DANVREILPRVTRPVTTYGTHEEASIRAENIVADHGKMHFTLVRKNGKTLRNEITLNL
                     PGHHYVLNALAAIAIATELNVPDAAILKALAEFRGVGRRFERYGEIPVNGGHFTLVDD
                     YGHHPIEMQAVIAAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVLSTVDALLLTEV
                     YAAGEAPIVAADTRSLIRAIRVNGKVEPRFVETTDELPATILDSVQPGDVVVVMGAGN
                     IGSVAAKTRELASA"
     gene            complement(21637..22737)
                     /gene="murG"
                     /locus_tag="VNZ84_06970"
     CDS             complement(21637..22737)
                     /gene="murG"
                     /locus_tag="VNZ84_06970"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWU66738.1"
                     /translation="MSRAYTLMVMAGGTGGHVYPAIAVADALHAQGWKIVWLATEGGM
                     ENRLIADKPYDKAMMTMQGVRGKGLMGWLTLPVKLARAFAQAREAIKQQRPDVVLGMG
                     GFAAFPGGVVARLAGVPLVIHEQNSIAGLTNKVLARIASRVLTGFPNALGTKGEMVGN
                     PVREVITTLPVPEQRFAEHHGVLRVLVVGGSLGAVALNTLLPQAFAQLPVNARPQIIH
                     QAGEKQIEALKKNYAEAGVAADCRAFIHDMAEVYAWADLVICRAGALTVAELANVGAA
                     SILVPFPFAVDDHQTTNAAYLQQAGAALLIQQRDLDVTQLANTLKTLDRNTCLEMASK
                     ARALARPQATQQVAEICQQLAIAKTNGKPGSV"
     gene            complement(22737..23900)
                     /gene="ftsW"
                     /locus_tag="VNZ84_06975"
     CDS             complement(22737..23900)
                     /gene="ftsW"
                     /locus_tag="VNZ84_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWU66739.1"
                     /translation="MLGPLMMNRERYNSPNYDVSLLWVVLSLLAFGMVMVYSASIAYA
                     DSSKMFGHNSTYFLVRQALYIVVGLVAAVIVFQIPMAWWQKMSPYLFIMGIVLLILVL
                     IPGIGKVVNGSRRWISLLIMNLQPSELMKLFIAMYASDYAVRKASNMHSFKKGFLPML
                     GVMVFVGVLLLKEPDFGAFAVTASIAFSIMWLGGVNLKVFLAMLGALPLAVIGLVVME
                     PYRLERMKGFLNPFDDPFDTGYQLSHALIAFGRGEFWGVGLGGSVEKLLYLPEAHTDF
                     LLAVVAEELGYVGVLVVVLLFAWLVIRAFSIAKEAVANERYYSALLAQGIGVWMGVQG
                     FINMGVNMGLLPTKGLTLPLLSYGGSGILANCIALSVLLRIDFENRRLQKGLN"
     gene            complement(23882..25255)
                     /gene="murD"
                     /locus_tag="VNZ84_06980"
     CDS             complement(23882..25255)
                     /gene="murD"
                     /locus_tag="VNZ84_06980"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWU66740.1"
                     /translation="MKKHFEQQRIAVLGLGETGFSALRWLRQMGAEVVMFDSRLHPSG
                     LDRLHQEFPDVEYHLGAFNASLLAAMDLIVASPGVSLREPAIVELRAQGKSVVGDVEL
                     FARFRAPYAKVIAITGSNGKTTVTSLVGEICKQAGLNTIVAGNIGLPVLDTLEMTAPD
                     VYVLELSSFQLETTYSLQADAATILNLSEDHMDRYDGMEDYAHAKARIYQQARIAVVN
                     RDDAPSAALAGQLPQVSFGVNPSPTINDYGLNDAGWLCAGNRHYIEADSLQIRGKHNI
                     ANALAAIALTQSIGIDKVSILHTVQSFSGLPHRVEWVANIDDVDFYDDSKGTNVGATC
                     AAISGMLKQGKPQKVVLIAGGDGKGQDFSPLREAVLHHARAVVLIGRDGPKIQQILHN
                     VQLPVVDAVDLTAAVHIAKTLAEEGDAVLLSPACASFDMFRNYEHRAQVFVEAVKAME
                     VACLGHS"
     gene            complement(25284..26387)
                     /gene="mraY"
                     /locus_tag="VNZ84_06985"
     CDS             complement(25284..26387)
                     /gene="mraY"
                     /locus_tag="VNZ84_06985"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWU66741.1"
                     /translation="MLLELAKWLAADVHGFHVFNYITFRAVLATLTALAISFMVGPAM
                     IRKLTEYKVGQAVRDDGPQTHLVKAGTPTMGGALILVAIAIATLLWGNLHNRFIWVVL
                     ITTLGFGAVGWVDDYRKVVYKNPKGLSAKEKYFWQSLIGFGVAGFLYATATTPEETTL
                     IVPFFKHLVFPLGAVGFIVLTYLVIVGSSNAVNLTDGLDGLATLPTVLVGSALAIFSY
                     VAGHAVFSKYLGIPYVAGASELMVFCAAMAGAGLGFLWFNAYPAEVFMGDVGALALGA
                     ALGVVAVIVRQEIVLFIMGGVFVVETFSVAVQVLYFKYTKKMTGTGKRIFLMAPLHHH
                     YEQKGWKETQVVVRFWIITMMLVLVGLSTLKLR"
     gene            complement(26396..27760)
                     /gene="murF"
                     /locus_tag="VNZ84_06990"
     CDS             complement(26396..27760)
                     /gene="murF"
                     /locus_tag="VNZ84_06990"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWU66742.1"
                     /translation="MISLHTIADVLKGTLQGGDAVVTSVDTDSRRAKPGQLFVALAGE
                     KFDGHDFLDQVAAQGAVAALVSKPVETHLPTVLVKDTRLALGQLSSWWRQQLALPLIA
                     VTGSNGKTTTKEMIAAIMQAHVGSADNVLATAGNFNNDIGMPLTLLRLRPAHRCAVIE
                     MGMNHLGEIDYLTRLARPDVAVINNAGTAHIGELGSRENIAKAKGEIFAGLSHDGIAV
                     INADSEFADYWRGLNKERRVLTFGLHPAANVRGEITDAESTFELHYQGDAVTLTLVVP
                     GAHNIMNALAAAAASLAAGASLAEVAQGLQGFAGVKGRLQQKVAANGARLIDDTYNAN
                     PDSMKVALDVLKSSGANTVFVMGDMGELGTDAEAMHAEVGRYAKESGIARMYALGKLT
                     QAAVQAFGAQAQHFASLEALVAALQHETTTNDVVLVKGSRFMQMERVVNALVESQATE
                     LVGK"
     gene            complement(27757..29217)
                     /locus_tag="VNZ84_06995"
     CDS             complement(27757..29217)
                     /locus_tag="VNZ84_06995"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWU66743.1"
                     /translation="MSKYIIPAPVHGITADSRQVEKHGLFLAYPGQHSDGRDYIEDAI
                     AKGVNTVIWDSEGFDWNPEWTVHNIAIADLKSQVGHIASQFYKDPSEQLWCVGVTGTN
                     GKTTVTHWLAQAYRFLQQKSAVIGTLGNGPIEDLQPTQNTTPGPVELQRMLATFVLDD
                     VRMVAMEVSSHGLDQGRVNGVAFDVAVFTNLTRDHLDYHLTMEAYQAAKRKLFDWQTL
                     SAAVVNADDTFGLDLIDALRKQGKKVLSYGLHQEADVCATAVTMHATGFEIQVHTAQG
                     RGDIQLHALGQFNVYNALAVLACLLANEVALPAALQAVSGLVPVAGRMQMFGGGDLPL
                     VVVDYAHTPDALEKALHTLRVQARGKLLCVFGCGGDRDSGKRSEMGRIADELADTVIV
                     TNDNPRTENPYAIIAAIAEGMMREVNVEVDRAKAIALAVSQASKGDVVLVAGKGHEDY
                     QEIQGIRYPFSDAEWVQNALKKSVPNQHALKQKVQA"
     gene            complement(29214..30947)
                     /locus_tag="VNZ84_07000"
     CDS             complement(29214..30947)
                     /locus_tag="VNZ84_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWU66744.1"
                     /translation="MVMLLKESQHKLVKLPAWRRRTLLVLLLLSFALLLGRGFYLQTL
                     HKDYLIKKGEAFSRRKVVLMPHRGKIYDRNFKPLAISLPVESVWVNPTDVQISAVQLK
                     QMAKLLGMPARELQQKVQQKKKEFVYIKRRVAPDVAKQVMAMKVPGVFSQKEYKRFYP
                     AAEVAAHIVGFTGVDDTGVEGMELYRNNVLSGTPGKRDFVRDRKGHVVEDLVAVKLPH
                     DGQDLVLSIDRTVQYVVHRELSRAVEKHKAKAAAAVVLDAQTGEVLALVNIPTYNPNN
                     PVNVASKLRNSAIVNIFEPGSTMKPVTAAAAMEFGPYQPDTKIQTAPGYLRIGTATIH
                     DAHPNQVLSVSQVIQKSSNVGSAKMALDLNREQLWNTYVQLGFGTPTKIGFPGEAAGK
                     VRDYKTWRPIEQATMSYGHGISVTLLQLARAYTVFANEGELLPVTLTKLSEPPVGRQV
                     FSAKVANSVKDMLELVVQPGGTALKAQVTGYRVAGKTGTAHKLGAGGYEHDKYVGSFV
                     GMVPASNPRLIMAVMIDEPSNGEYYGGSVAAPVFSAVMNDVLRMLVIPQDGNVVPATT
                     SADAAEVKEAM"
     gene            complement(30968..31246)
                     /gene="ftsL"
                     /locus_tag="VNZ84_07005"
     CDS             complement(30968..31246)
                     /gene="ftsL"
                     /locus_tag="VNZ84_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HWU66745.1"
                     /translation="MLRLNLILFALTIAMALGVVTAQYKARKLYFELDRQEVLTKQYM
                     TEYDQLQIEQSTWAMHSRLEDFATTRLHMHSPSMQQTQVIMIDVPAAP"
     gene            complement(31268..32257)
                     /gene="rsmH"
                     /locus_tag="VNZ84_07010"
     CDS             complement(31268..32257)
                     /gene="rsmH"
                     /locus_tag="VNZ84_07010"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWU66746.1"
                     /translation="MTRGAKSAPASLTETSVSPVAASHITVLLHEAVDALNVQADGVY
                     VDGTFGRGGHSRLILSKLGSKGRLIGLDRDLAAVAHGQTIADARFTMVHAHFSAMATV
                     LVGLGVQAVDGILLDLGISSPQIDEGVRGFSFRFDGPLDMRMDQSRGKTAAEWLVTMS
                     EKQLGEVIRDYGEERFAKQVARAIVKEREDGRAITTTGQLAKVVAGAIPKNEPGQNPA
                     TRTFQALRIFVNQELEELSLVLPDCLKLLCPQGRLAVISFHSLEDRIVKRFIQSEEDR
                     DNLPAGLPVRAKDLPQPRLVSVGRAIKPGKAEVTANVRSRSAVLRVAERTHVQ"
     gene            complement(32254..32700)
                     /gene="mraZ"
                     /locus_tag="VNZ84_07015"
     CDS             complement(32254..32700)
                     /gene="mraZ"
                     /locus_tag="VNZ84_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWU66747.1"
                     /translation="MFRGAVQLSLDAKGRLSVPAKHRDALTAGGQGEVVLTAHPHRCL
                     ILYPQAAWEPIETKLTSLSSFNPQTSGIQRMIVGQADVMHLDSAGRLLVNAVLRKFAK
                     LDKDVMMVGQGSHFELWDVAAWDQQLESLMATDQLQIPQELEGFSL"
BASE COUNT         7903 a         9475 c         7882 g         7548 t
ORIGIN      
        1 tccggggcgg tttccttgcg gctttcgtca aagcggccac ggtattgcag ttcaaaattg
       61 ttattaaagc cgaagaaatc cggcgtgcat accgcatcat aagccttggc cacttgctgg
      121 gtttcgtcca gcaaataagg gaaaggcagc gccaattcgt cagacagttt tttcatgttc
      181 tccggactgt cttccgggta gtcagagggg tcattagaca tgattgccac ggtattgaca
      241 cctagcgcct gtagcgcttt tacatcggca atcaggcgtg gcaggatcgc ttttacatag
      301 ggacagtggt tgcagataaa catcaccagc aaaccgtttg gacccatgcc gtcgttcaga
      361 ttgaccagtt ttccgtccac atcgggcaaa ttgaatgtcg gggctttcca gccaaaatca
      421 catactgggg gactaatgct tgccatcaga gttgctcctg ccattaaatt caaaccatta
      481 tggtacgtga ttaagccgcc gaggtgaatc ttggctggtc gccgtgcctg tcccctggca
      541 ttttggttac aatgccagca tgagtaattt acgatttcat agcccaactg cattagcagt
      601 cggccagcaa gcgcccctga gtgaaaatgc catggcacat gcagtgcggg tcatgcggct
      661 gaaggtgggc gatacactga cgctgttttg tggtgatggc tttgactacc aatgcgcact
      721 gaccagcatc gagaaaaagt ccgcacaggt tgaagtattg agccacattg aagtcagcaa
      781 tgagtcaccg ctgaccatcc gcttactgca aggcatttct agtggcgacc gcatggatta
      841 caccatccag aaagcggtag agctaggcgt gaccgagatt tacccactca gtacggagcg
      901 ctgtttgacc aaacttagtg gcgaccgtgc cgagaagcgc attgagcatt ggcaaggagt
      961 tgccattgcc gcctgtgagc aaagcggccg cgccattgtg cctacagtac atgcaccact
     1021 cacactggcg caatggttga gccagcacgc caccccagac tgcctcaacc tgttgctcaa
     1081 cccggtcggg gcaaaacgtc ttgcagcatt gagcaaacca gccaatgcca cgcatttgct
     1141 gataggcccg gaaggtggcc tgagccctgc ggaaatagcg cttgccacgc aacaccactt
     1201 tcagtctatc gtgcttggcc caagaatttt gcgcactgaa actgccgccc tcaccgccat
     1261 cgccagcatg cagtcacttt ggggtgactt ttaggcacgc atgtttcaag tcatgttaca
     1321 gatggcgtta ctgattgccg ctggcatgct ctggcagcgg ctggcgcctg accatatccc
     1381 agccatttct cacagacgtg cactgacaga tctcgttttt tatattctgc taccggcact
     1441 ggtgattgat gtcttgtggc aagcgccgct ggacgcagat acctttagca tccaactgac
     1501 cgcaatttcc cggctgtccg tggcggcatt actgatgtgg ctgtgcctgc aggtactcaa
     1561 aagattcttt gatgttcccc gcgcgcagca gggcgcgttg atgctggcag cgacatttcc
     1621 taacgccacc tatcttggct tgccggtcac cagcgaagtg ctgggagatt gggcgcacga
     1681 gatggtgctc aaatttgacc tgtttgcctg cacgcccatt ctgctatcgc tgggaatgct
     1741 gctgggacaa cactatggcg aggctgccac caaaagcaat ccgttcaagg aactgattcg
     1801 tgtaccgcct ttgtgggcat tggtgattgc ctgcgcattg aatctgctgc atgtccctca
     1861 acctgaaatg attgcgcatg ccctacatac gctggccggt ggcgtgattc cgctcatgct
     1921 ggtcgcactg ggcatgagca tccgctggga tacctttaaa ctcagattga ttccgctact
     1981 attgcccgta tgtgtaattg gtttactgat tgcacccctg gcggcacgtt atgccgcaat
     2041 tgccctcggg ctgcatggca accagttaac tgcctgcatc ctgctcgcgg ccatgccaac
     2101 catggtcttt ggtattgtca tttgtgagcg ctatcgcctt gatggcgctc tgtatgccgc
     2161 cgccgtcttc atgagtacct tgctcagcat cgccagcctg tcactgtggt tcgtcatact
     2221 tcagccataa acgctccata ttatttctct tcaaactcat gaaatctcgc atgccgtgtg
     2281 aatttttatc aggcacgctg tcatacgtca tatgacgagt agccaacagg ccaggctgtt
     2341 tttaagatac tgctaaacag tcattctttc gtattgtgta tttgggatta tcagaagatc
     2401 atgccgaaaa ccacgaccaa ccaatatatt gcgccgcaac ccacgccgcc agccacgctg
     2461 gtcagtctgc tggctgaaac aggcgtcacc atcaatggca atgcgccttg ggatatccag
     2521 gtgtatgaag aaagcgtata ccgtgatgtg ctgaccaaag gtacgctggg actggggcag
     2581 gcatttattg aaggccgctg ggactgtaag cgtcttgatg agtttttcca ccgggtcatg
     2641 cgtgcagaca ttgatgaaaa aatgggcggc atggcaaaac tcaaactgct gggcgaaatc
     2701 atacggcact cgtttttcaa cctgcaaaca cctgaccgcg cctaccaggt ggcgcaacag
     2761 cattacgata ttggcaacga tgtatttgaa gccatgctgg acagcagcat gagctattca
     2821 tgtgcctact gggagcatgc cagcacactg gaagaagccc agttcaacaa actggatatg
     2881 atttgccgca agctggacct gcaaccaggt gaccgggtac tggaaatcgg ttgtggctgg
     2941 ggcggcctgg ccaagcacat ggcgaaatat tatggggtgc aagtggtcgg catcacggtg
     3001 tcagtcgagc aacaggcact ggctaaagag cgttgcgctg gtttggcagt ggatatccag
     3061 ctcaaggact accgtgagct gcatggccag tacgacaaaa ttgtctctgt cggcatgttt
     3121 gagcacgttg gtccgaaaaa ctacgaggtg tactttgcaa ccgcgaaacg cctgctgaag
     3181 gacaacgggc tgtttttact gcataccatt ggtaattaca aaaccgccct gcacacggac
     3241 gcctggatag accgctatat tttccctaac ggcaaattac cttctgccag tgaaataacg
     3301 aatgccattg agcaagaatt cctgattgag gactggcata actttggccc ggattatgac
     3361 cgcacgctga tggcgtggtg ggacaaattt aaccgggcct ggccggaact acagtttaaa
     3421 tatgatgaaa gtttttaccg tatgtggaaa tactacctga tgtgttgtgc aggttatttc
     3481 cgctcgcggc aagggcaact ctggcaactg gtactaagca aacggcagag caaccgtatt
     3541 taccgctcgg tacgtccgct gccagcaagt tgatgttgac gtagtctctc tgttcttcct
     3601 tatatgacgg cttttgccgt catttttttg ccccaggatt gtaataaaag catttcttca
     3661 ctaccgatac gcaccgatcg aactcaaaca atcccagcct ctgacactgc ttgcttattt
     3721 cttcatctgc aagctgatac agaccttcgc ccaatcgaga aaacacccta tattatttgc
     3781 aataaaaacc atttttattt attattttgc aaattaatct taatttaacg acaaatgctt
     3841 ggatcaaaca ttagagattt gcgtaaatcg caaaatttaa ccttgcaaca gttggccgcc
     3901 cgcatgggta ccgatgccag taatttgtca cgcatagagc gcggcgaaat cggcatctcc
     3961 gaagccttgc tgcgcgcggc cgcacaagcc ttgaacacca cacccggccg cctgtacgac
     4021 gcaaacctga caccggtgtc gctcaccaac acacctccag cgattctttc gagtgccaac
     4081 agcaaggatt ttgtacactg gttccgctca gcagcgccct acatccatgc ctttggcggt
     4141 agcacgtttg tcattgcttt tggcggggaa gtggtcagcg aacaacagtt catcgccctg
     4201 tcacacgacc ttaacctgct tgccagcctg gaagtcaggc tggtactggt acatggggca
     4261 cggccgcaaa tcgagcagcg gctcaaacgc gacaacctga cacccaaact acataatggc
     4321 cggcggatca cggacgatgc agccatggaa gcggtaaaag aggccaatgg tgccattcgt
     4381 gtggaaattg agtcactgct ctccatgggc attgccaact caccaatggc cgggagtgac
     4441 atccgcgtgg ccagcggaaa ttttgtgacc gccaaacccc tgggcgtccg cgaaggtgtg
     4501 gacctgcaac ataccggcga agtgcgcaag atcgatgcca taggcatcca gaaacggctg
     4561 gatgacggcg agttggtgtt attgtcacct atgggctact cacccaccgg cgaaaccttc
     4621 aacctgacac tggaagatgt ggccgtgagt gccgcgattg cgctagatgc ggaaaaactg
     4681 attttcctca tggactcaga aggcgtacaa aatttacgtg gtgaactgct acgcgaaatg
     4741 acctctgcca aagccaagaa cctgttacgc aatgtgcagg aaagtgacga gccgattcat
     4801 tactcggaag atgtgaatta ctacctgcct gccgctgtac gcgcctgcga gcatggcgtg
     4861 gccaggacgc acctgatttc gcgccacatt gatggcgcca ttatccagga gctgtttacg
     4921 cggcagggca taggcaccat gattaccgag ctacccttgg aaaccatgcg ccaggccaat
     4981 attgacgatg tgggtggcat cctgatgctg attgagccac tggaaaatga aggcatcctg
     5041 gtacggcgcg ggcgcgaacg catagaaatg gaaatcaact atttttatgt catggaacat
     5101 gataaccgcg tcattggctg tgcggccctg tatccattcc cacaggaaaa aatggtggaa
     5161 tttgcctgcc tggccataga tccggcttat cgtggcggag gccgtggcga caaattgttt
     5221 aacttttgcc agcaacaagc cagccaaaaa ggctttaaaa agctgttttg cctgacgacg
     5281 cgtaccgagc actggttcct tgaacgtgga tttaccgaac tggaaataga ccaattgccc
     5341 caggaaaagc aaaaactcta caattttcag agaaaatcca aagttttcat caaatcgtta
     5401 tgagttttta gctaaaattc aggctgaccc cgattgatcg caatcgcctg atgattgaat
     5461 gaaagtttga ccctatgcaa acctctgccg ccagccaaaa agcgcaaacc ctgtccgaag
     5521 cgttacccta catcaaacgc ttttttgaca aaaccatcgt gatcaaatac ggcggcaacg
     5581 ccatgaccga tgaccgcctt aaagaatgtt ttgcgcaaga cgttgtgctg ctcaagctgg
     5641 tcggcatgaa tccggtcgtg gtgcacggcg gcggcccgca aattaacgaa atgctggaca
     5701 agcttggtaa aaaaggcgag tttatccagg gcatgcgcgt gaccgacgag gaaaccatgg
     5761 acgtggtaga aatggtgttg ggcggccagg tgaacaagga aatcgtcaac ctgatcaacc
     5821 gtcatggtgg taaagcggtt ggcctgacag gtcaggatgg caacttcatc catgcgcaca
     5881 aactgctgat ggaagacctc aaggatccaa ccaaaatgat tgatgttggc caagtgggtg
     5941 aaatttcagg catagacccc agcatcatta cctttctgga cagtggcgac ttcattcctg
     6001 tcgtcgctcc cattggcgtc ggcaaggatg gtgaaaccta taatatcaac gctgacgtgg
     6061 tggcgggcaa actggcagaa attctcaatg cagaaaaact cattttgttg accaacacgc
     6121 cgggcgtgct ggataaaagc ggccaactat tgactggcct gactcccaag caaatcgacg
     6181 acctggttga agacggcacc ctgagtggcg gcatgctgcc caaaatcggt tcggccctgg
     6241 atgccgcccg cagcggcgtt aagtctgtac acatcattga tggccgcgtg gagcatgcct
     6301 tgctgctgga agtgctgact gacgaaggtg tcggcacttt gatcaaagcc aaataagcaa
     6361 ccactcacct tcaccgtaaa aagcactaca acgtatgtca cgcagggtgt ggattttcga
     6421 tctggatgac accctgcaca acgcatctgc ccatatcttt ccggtcatga accaatgcat
     6481 gacccattac atcatgcaga cgctagacat ggaggaagcc gcagcccacc aattacgcca
     6541 gcattactgg cgtatttatg gcgccactct caaggggctg atgcggcacc atggtgtgaa
     6601 tccacatcat ttcctggcag aaacacacgc cttcctgacg gacgatatgg tacaaaccac
     6661 gcgccagttg aggcaactac tcaacagctt acctgggcgc aaatgtgtct ttaccaatgc
     6721 gcctcgcgcc tatgccatgc gcgtccttga agtgctgggc attgccgatt gtttttcact
     6781 ggtctttagc gtcgaatcca gccgctttca tgccaaaccc agcatgcgcg ggttccggat
     6841 gttactcagg caattgcgtt gccgtgcggc agattgcacc ctgtttgagg acagcctgcc
     6901 cgccttgatg acggccaagc gattaggcat gcgtacggta tggattagtc gccgcttgca
     6961 caaaccaaac tttgtacaat ataggttgcc acatgtgttg gcactcactc acagcgctct
     7021 caaaaaaggg caaaatgcat tcccgtgcta ggcgaatgcg gttaaaatac ctgccaaata
     7081 acaacatgaa tatgggctga tcctggaaat cattatggca acgatcaata aagaagcacg
     7141 cgcaaaccgc aagcagcaga ttctggaaac actggcaggc atgctgcaaa atccacgcgg
     7201 cgaaaagatt accacggcag ccctggcagc caagctggag gtctctgaag cagcgttata
     7261 ccgccacttt gccaacaagg caaaaatgtt tgaaggcctg attgagttta ttgagggcgc
     7321 actctttggc gtcatgaata aaattacgac ggacgaaacc gatggcctta aacaggtaca
     7381 gctgatgctg cagaccatgc tcaagtttgc cgaacgcaat cctggcatgt cgcgcgtatt
     7441 gatcggcgac gcactcgtga atgaagatga atccttacag gcccgtatta accagttgct
     7501 ggaccggctt gaggccagcc tcaagcagtc attgcgtatc gccgaagcac aaggccataa
     7561 ggttgcggat gcgggaattg cggccaacac cctcatcagc tttgtgctgg gccgttggca
     7621 cgcctttgcc aaaagcggat tcaaacgtaa accgagtgac aaccttgaac agcatgtcac
     7681 acaactgatt accagctgtt tgcaataaaa gatgtagaaa gtgtcataga tggaaaacac
     7741 cgtttttggg atgaacgaag cccagctcac cgagtttggc atgagttggg gcctcgctgg
     7801 tctgattctg ttcatcgtgt ttattgtcgg caacctggcc tggaagtcca aagcgggtaa
     7861 aaccgggaca tttgcgttat ttcttgccct caccttgggc atggttggct ttctggctaa
     7921 actgcttatt cagcattacc tggatatcca ttaatcctgc tgcagggagt ttctgcactg
     7981 cggccatcgt tcacaaggaa cgtttatgca gcagatactc gtctattctg actcactgag
     8041 ttggggcatt attcccaaca cacgccagcg actgccattt gaccagcgct ggcccggcgt
     8101 gttggaaatc atactcaatc agcccggtag ccatttcagg gtcattgaag actgcctcaa
     8161 cggacgtcgc accgtgttcg aagacccgta taagcctggg cgcaatggcc tgcaaggttt
     8221 ggcgcaacgt attgaaatcc actcaccttt agcgctggtt atcctgatgt tagggaccaa
     8281 tgactttcag tctatgcatc tacaccaggc ctggcatgcg gcacaaggta tcgccagcct
     8341 gatccacgcc atacgtcatg cgccgattga acctggcatg ccggtgccgc ccatcctggt
     8401 ggttgcgcca ccggcagtga cacaccccca aggcctgatt gcggaaaaat ttgccggtgc
     8461 cgaagaaaaa tgcaagggcc tggccgaagc ttatgccgct gtcagccagt cattggactg
     8521 cgctttcttt gatgcaggca gtgtcgtaag cagcagccaa atagatggcg tgcacctgga
     8581 tgcagcccaa cacctcattc tggggaaagc cattgccagc caggtaaatc gtttaattgc
     8641 gtaaatatcg cggccggact attgcgatgc aggcaacagt tcgttagtct catttaatca
     8701 gtcacagtaa acacttgcac gatatggaca actccccacc tagcgacagc gacacctaca
     8761 aaacactgct cgaatctacc aaggcgatcc cgtggaagat agactgggcc agcaaacaat
     8821 tcacctatat tggcccgcaa attgaaccct tgctgggctg ggcacctgag agctggcact
     8881 ccgtagaaga ctgggtggca cgtatccatg aagaagaccg tgaacgcgtc tttaactttt
     8941 gcgtgcagca atcgctacag ggcctggatc atgaggcaga ctaccgcgcg ctggcagcag
     9001 atggcagtta tgtctggatt cgcgatgtgg tgcatgtcct gcggacaccc aatggtgagc
     9061 cggaagccct gatcggtttc atgtttgata tcagtgagcg caagaaaacc gaagaacaat
     9121 tattgatgat gcaaaaacag ctggaagagt tttcgttcaa ggatggactg accggggttg
     9181 ccaaccgccg gatgttcgac accgtccttg aacgcgaatg gctggaggcg aggcagcatc
     9241 agcagccact gtcattaatc atgattgata ttgactactt caagcaattc aatgactttt
     9301 atggtcattt gcaaggtgac gaggtgctta aacaggtggc caaaacgcta tcgcatgccg
     9361 gggtacgcgc caaggacttc ttcgcgcgat ttggcggtga agaattcgcg ctgattttac
     9421 ccgagaccaa tgcgctgtct gccagcaaaa ttgccgagcg ctgccgcaac ctggtgttca
     9481 aggagcagat tccgcacgca aaatcagagg taagtcagat cctgaccatc agcgtggggg
     9541 ctggcagcat cacacccgga ccggccgatg acctgaaggc ctttgttgaa tcggtagatg
     9601 cactcatgta ccaagccaag cacgatggcc ggaaccgcat tgtggtaaaa agtgcctgac
     9661 gcagacacgt gaacaactta atgactcacc tcaatgatgg aggtgcaatg cagactgtat
     9721 cgcttgtata cgggcttctg cgaccgattg cttgatatgt gccggggaac tgcatgcctg
     9781 cgcaatagca cccgcatcaa ccgtcagcgc tgcgtgcaaa gccgctgaca aatgtctagc
     9841 atcgtctaaa gcacagtctt ccaggcccag gcgaccgcgg ctatcgcatt cgcatgccag
     9901 taaaaaatcc tcgaaccgcg ttcgctggcg gaacgcatcc agctcttcca gaaactgcag
     9961 cagggtactc gccttcattt gccgggcagc atgcagtttg ccatggagtt tggccacaac
    10021 cagcgcaagg tccttgcaat cattaggtac ccgcaaccgc aaaaccactt cgcgtatcag
    10081 atccacactg cgtaactcgt ggccgatatg gcgcggcaac acctcagcag gtgtcgtccc
    10141 cttgcccaag tcgtgtgtca gtgcggcaaa gcgcaccggc aagctatagt tctgcttcgc
    10201 tgcataatca atcaccatca tgacatgcac cccggtatct acctcaggat ggtaagtcgc
    10261 cgtttgtggc acgccccaca aacgatccag ttccggcaag atcacctgca acgccccaca
    10321 atcgcgtagc atttcaaaca tgcggctggg cttcttttcc atcagccctt ttgcaagctc
    10381 ctgccagaca cgttcaggca ccagtgcatc gacttcaccg gcagccacca tatcgcgcat
    10441 cagggtcagg gtctcagggg caacggtaaa atcagtaaac cgcgccagga aacggcctgc
    10501 tcgcaatatg cgtacgggat cctcactgaa ggcgtccgtc acatggcgta gtattttttt
    10561 ctggatatcc agctgcccgt gaaaaggatc aatcaaacta ccatcttcag cctgggcaat
    10621 cgcattgatc gtgaagtccc gccgcgcaag atcttcctcg agagtcactt ctggagccgc
    10681 atggatgcta aagcctttat agcctttagc ggttttgcgt tcggttcggg caagcgcatg
    10741 ctcttgttgt gtttgtggat gcaaaaaaac cgggaagtcc ttgcctaccg gccggtaacc
    10801 ggccgccagc atctgttctg gtgtcgcacc cacgaccaca taatccctgt ccttgaccgg
    10861 caggccaagc aggctatcgc gaacggcacc gcctacctga taaatttgca tggtgaacca
    10921 cagccagcgt taacgcctgg cgttaatgac tcgccctgct gcggcgcgga aatgatcgta
    10981 agcgccacgg ggattgtttt tgaccagata gccagagatc accgcaaaca tgtcagtagg
    11041 caccacacgc aattcattag ccatgggttg ctggcaaaca ttatccactt gcatggactt
    11101 cacgttatcc ctggaaatca gcttgatcgg cagtaactgc ataaagctac cttgtaataa
    11161 cgacatcttc agactcaaac caatgattgg gcgttggaca tggatggtct tcatcactgc
    11221 ctcaataatc tgctgcaaag tcatgatcgc agggccaccc agttcataag tctgaccata
    11281 agtcgcaggc tcatccactg cattcaccat ggccgcggcg acatcctcta cccagatcgg
    11341 ctggaacttg gcctgaggca tagccagcgc caggacagga atccattgga tcagtttggc
    11401 aaacagattg ataaaacggt cgcgcgtccc aaaaatcacc gacggcctga aaatcgtgac
    11461 attcagtttt ttgctgaact ccatgaccgc ctggtcaccg gcagccttgc tgcgcaggta
    11521 ctcgctaggg gcattcacgg aagcctgcaa tgcactcatg tgcaacaggc gcgggatacg
    11581 tagctcttcg cagagctgcg caacgcgacg cgggaactgg tgatgcattt gctcaaagcc
    11641 gttatcgcgc gtttgatgaa gaataccgat caggttgatc acaacatcgc tgccttgcag
    11701 ttgcgctttc aaggcagcat ggtcatgtat atcacactcg accacttgca ccttgggtag
    11761 caagatcagg tgcttggctt gctcgcgtct gcgggtcaac acctttacct ggtagcctgc
    11821 ctgatccagc ctggcaacca cactactccc tacaaaaccg ctaccaccta atacagtcac
    11881 tgtcttcatc atgcttattc ctgatacgtc ttgcgattgg ccctattgta ccaaaataca
    11941 gatagaagct actcctgctc cgaaatactg atcgtcgttg tcacagattc cacctcagcc
    12001 gtcgtacgcg cggggatagt ccccagccgt cttttaagtg tcagcatggg caagcccaac
    12061 ctgggggcat acatatgagc attcgccagc acttttttga catagttgcg tgtttcactg
    12121 aagggaatgg tttcggcgta aatcgcgcct tcgaggggcg tactaccggc ccagcgtctg
    12181 gcgcggctcg gcccggcatt gtacgccgcg gttgccatca cttcctggcc gttaaaagta
    12241 tccagcgtat agcgcatata gtaagtgccc aggtttacgt tggtatcaat atcatgcagc
    12301 atgccatcat ggtaaccatt caaaccgagt tttttagcaa tccatttggc cgtggcaggc
    12361 atcacctgca tcaagccacc agcacccacc gatgacttgg cataatgcat gaaacggctt
    12421 tcctggcgga tgatgccata gacccaggcc tcatcaatgg cacggttacg cgaagccttt
    12481 tgtaaataat cccgataagg cgtgggatag cgcaattcaa aattgtgtgt acggctggtt
    12541 ttgtctgctg ccagcacggc taagtcatac cagcctttga gcaaggcata ttgcgccgcg
    12601 acaatccgtg taggatcatc gagcacttcc agcaaataca gccactccaa cctggcctca
    12661 tagcgtgctt caacatcaaa cagcacttcc atacgcttga ccaccggctg cctggcaaac
    12721 tggcgcactt cttcatcggt tggctggtaa gtggccatgg gttcacctgc taccggcccc
    12781 aagtcttccg ccgccagcca gccataaaaa tgccgttctt gtgacaattt ggccaagatc
    12841 tgattagcat ctgcgatctg ccccttggca atcagcgcac gtgctttcca gtatcgccag
    12901 gcagactcat cactgagtgc agcaggcatc tcattaatca ctttcagcag ccgttcccag
    12961 tcctgctggc gaagggcact ccgggcatac cactcccact gttcactact gagaatcact
    13021 gcgttgacct tgctgaaccc ctgcaaggca tctgactcgt gtttgcgtgc cgcttctagc
    13081 gcaatatagg cctgtgccag ttgtttttct tcgttatcca gaaaaccact atatttctgg
    13141 taactggccc tggctacgct ggtatctgct ttggcctgac ggtggatcgc atagaggtaa
    13201 agcgcccgct gataagctga acgctgctgg atattgccct ttttcagggc cagcgccgga
    13261 ttctgtgcca tgtcatcaat ttgcttgaat aggccagcat cggttttact gctgcgcttg
    13321 gcaagcactt tggccaagcc cgttttattg ctgaacaagg catcgcggta caaggtgagc
    13381 aacagctttt catcgagcac accaacctgc tgcaaagcct ccatcatgcc ctggcaatct
    13441 gctggtaaat ccttgccagt cagcagtaat tttttgccat cctcataagc attcgggtca
    13501 tccagtgcaa taccggcctg tacctggtag cattggttac ccagatcatt gaaactgtaa
    13561 tacatcggga tttgcgcccg catttccaca aactggtccc aaaactgcac ccgccccagg
    13621 cgcttcagcc acaactcgcg cacacgttgc gatagcaggg attcgttgtt ctcctgtaaa
    13681 aaggcctgca cttggtcata agacagctga tccagggtca ggatcatttg ccagtattgc
    13741 agataaggta atagcgggga acggcgctgc tttagagttt cggtggcttc aattaactgg
    13801 ggcaggttcc tcgcctggta ggcctgtttt gcctgcaaat agacttcgcc atccccagca
    13861 aacactgact tggtcatcgc cagtaagcag gcagcaaaaa tgaggaggga agtgagtcta
    13921 gtcttcatgc gtcaacaatc acgtgcatca cgtgaggaca atcaaattct ggccaatcga
    13981 ctcagaaaga aaaacagtaa cagtagcagg atgacgataa aaccaccgcg ccatacgctt
    14041 tttaaccagc gccagtaacg ggcttggccc gtttgccatc ccagcaacac caataccagc
    14101 gtactgacca ccagcactgt aaataaaata cgtatccacc acataatgtg tacggcttat
    14161 cctatcaggc cggtaacaaa ggggccggac taaaatagtc acgctgttta atgaaaccta
    14221 ccggggcatg tgcatcatta tcaaaggtgc agtattccca agcggtatca tctgccagca
    14281 gtgcgcgtaa taactgatta ttcagggcat ggcctgactt gaaggctgta aactggccaa
    14341 taatcgggta ccctaggata tacaggtcgc caatcgcatc caacaccttg tgcttggcaa
    14401 actcgtcgtc ataacgcaag ccaccaccgt tcaatacctt gtattcatcc agtacgatcg
    14461 cgttgtccag gctaccacct ttggctaacc cgttggcccg caggtattcc acctcgtgca
    14521 tgaaaccaaa cgtgcgcgca cggctgattt catcaacata gctctggcta tcgtaatcaa
    14581 tggtcacggt tttaccggaa aactcgaaca cgggatgtgc aaaatcaatg gtgaattcag
    14641 ctttaaagcc atgatgtggt gtaaattttg cccatttatc accctcttgc acttctatcg
    14701 gcttgaggat acgcacaaat ttcttggcag catccaactc ttgcagacca gctgtctgca
    14761 gcaaatagat aaacggcccc gaactgccat ccatgatggg cacttcggca gccgtcagct
    14821 ctatcagcac attgtctacc cccaaaccag cgagggcaga catcaagtgc tccacggtag
    14881 ccacgcgcgc acctgaaaac tctagggcgg agctcaggcg agtgtcgttc actgctgtcg
    14941 gggcgacccg catggcaggc gtatcgggca aatcgatgcg ctggaacacc actccgtgat
    15001 cagcgggcgc aggtttgagc gtcagcgtga ctttctcacc gctgtgtagc ccaacgccag
    15061 tggcgctgat cggttgttta agcgtgcgtt gctttaacat ggttttctct gtattaactg
    15121 attggctcca ttataagcca atcactatca ttactaatca ttcacaggcc catcaaggcc
    15181 tgtcaattaa tcagcctgtt tacgcaaaaa tgccgggata tcgtactctt cgatcccgga
    15241 gtttttcatc gcttcaactt gtgcacgacg gctgttgctg gtaaacacgg caggctcatc
    15301 ttcttctacg gcgctgaaga taggctggtt ggttgtacca tcacgcagcg tagtcatgac
    15361 cttgagctcc ggcttggcct ggcgtttacc cagtgaaccg ttcaaaccgg tggccaccat
    15421 ggtcacacgc aggttgtcac ccatgctttc atccatcaca ttacctacga tcactgttgc
    15481 atcttctgca gtgaactctt tgatggtgtt catcacatca tagtattcac gcattttgaa
    15541 gctgccgctg gcgctaatat taaccagcac accgcgtgcg tttgccaggt tcacgtcttc
    15601 cagcaatggg ctggcaacgg cctgttctgc agcgatacgt gcacggttag ggccgctggc
    15661 ttctgcggaa cccatcattg ccatgcccat ttcggacatc acagtacgta cgtcagcaaa
    15721 gtcgacgttg accagacctg ggcaattgat gatttcagca ataccggaca cggcgttatg
    15781 caacacgtca tttgcagcac gataggcctc aaccacagtc acgtcttcac ccaatacgtc
    15841 catcaacttt tcgttaggga taacgataag agagtctacg tattgtgaaa gttcttccag
    15901 accttcctgt gccactttgg cgcgcttgcc ttcgaatgcg aatggcttgg tcaccacagc
    15961 caccgtcagg atacccattt cacgcgccac ttcagcgatg atcggtgctg caccggtacc
    16021 agtaccgcca cccatacctg ctgtaataaa cagcatgtca gcgccatcaa tggtttctgc
    16081 aatgctgtcg cggtcttcca aggctgcttc gcggccgatt tccggcttgg cacccgcgcc
    16141 caggccttta gtgatgtcag aaccaatttg cagtacaacc ttggcatggc tcttcttcag
    16201 tgcctgcatg tcagtgttgg cgcaaataaa ctctacgcca gtcacgctct tggtcatcat
    16261 gtgctcaacc gcgttaccac cgcaaccacc gacaccaatt accttgatta cggcgccgtc
    16321 attctctttg tcaaaaatct caatcatact aaccctccat tgcctattat ttgagtgatt
    16381 ggtttgcccc gcacactcgc cctaaaacac attaaaaaaa cgactcaaac taaaaattac
    16441 ctgtaaacca gcttttcatc cgttccagca acctgcctac tgacccagat tgcaagttac
    16501 ccaccatttg tttttctacc tgctgcttgg ccatcagcaa caagcctatg ccggtggcat
    16561 aacgcggatt acccaccact tcggataacc cttccacgcc cctgggtaca cccaggcgga
    16621 ccggggtgtg gaaaatctct tcacccaact caaccatgcc gcgcatctgc gccgcaccgc
    16681 cggtaatcac aatgccggag gcgatcatgg actccatgcc actgcggcgc agctcttgct
    16741 gcacaaactc gtaaatttcc tcgacgcggt cttcaatcac ctcagtcagg gcctggcatg
    16801 acaactgacg tggctcgcgg ccatccacag caggcacttc caccacttca cgtgggtcgg
    16861 ccagctggcg cagcgcacaa ccatgcttaa ccttaatttc ctctgccgac tgcgttggcg
    16921 tacgcaaagc caccgcgata tcgttggtaa tctggtcacc cgcaatcggg atcacagcgg
    16981 tatggcgaat ggcaccattc ttgaacaccg ccaggtcagt cgtaccgcca ccgatgtcta
    17041 ccagacaaac acccagctct ttttcatcat cggtgagcac agactcactc gaagccaagg
    17101 gttgcaaaat gaggtcactc acttcgagac cgcaacgctt gatgcacttg acgatgttct
    17161 gtgctgcagc aatcgcacct gtgacgatgt gcacgcgcac ttccaggcgg ataccgctca
    17221 taccgagtgg ctcgcgtaca tcttcctggc catcaataat gaattcctga gtcaggatat
    17281 gcagtatctg ctggtcggcc ggcaaagcaa tcgcacgagc cgtttccacc acgcgatcga
    17341 tgtccatctg tgagacttcg ctctccttga tcttcaccat gccatgcgag ttgaggctct
    17401 taatgtgact accagcaatg cctgaataca cagtgttgat cttgcagtcc gccatcagct
    17461 cgacctcttc aatcgcgcgc tggatggcct gcatggtgga atcaatattg accaccacgc
    17521 cttttttcaa gccacgggaa gcgtgttgcc cgacaccaat caccttcaat gtgccttccg
    17581 gcaaaatttc tgccgcaatc gccacaattt tggaggtgcc aatatccagc cccacgatca
    17641 aatttttatc ttctctgact ctactcatgt cttttccttg accttctttc tggcagtctt
    17701 attctttaag cacccgggcg tggcatacgc agtgcaaagc cattcgggta gcgcaaatcg
    17761 gcataacgcc agtcactctc tgcaatattt gcctgctggc ggtatgccag cacaaactgt
    17821 tgcaatcttt gttttgccgc ttcacgtccc agcatcatct ggcggccatc tgcggttcta
    17881 atctcccagg cgcggcgtga agacaagctc agttgctgaa tttccagcgg cgtctctgtc
    17941 agcatctgcg caaaactggc atagccttgc gccacttcct tcaccgcatc cccggggcca
    18001 taaaaggttg gcaattgctc atcagaagcg gcctggaaca actcaccatg cttgttgacc
    18061 agtgccactc ctccccagcg ccccagcgcc tgatgctctt caatttgcac cacaatcgtg
    18121 tctggccact ggcggcgtac agtaactttg cgcgcccagg gcagcttctc aaacgcgatg
    18181 cgggtacgtt gcaagtccag cgtataaaaa ttgcctttca ggtacttgct gacaatcaac
    18241 tggatctgct catgattgac atgatccaga ttgccttcca cctgcacctg tttcactgga
    18301 aaaatcggca ggtgtatgac cacaaacaaa gcggcgtaca gcagcatcac cactgccagt
    18361 gaaaacagca ggttggcaat ccagttcagc aatgttggcc tatcccacat gcccgcagtt
    18421 atcctcttaa accttatcca gtgtttgttg caaaatacga acgactagtg catcaaagtc
    18481 catgccggcc gccttggctg ccatcggcac caggctatgg tctgtcatgc ctggactggt
    18541 attcacttcc aggaaatagt gtttacctgc ctcatccatc aaaaagtcca cccggcccca
    18601 gcccttgcac cccagtactt tgaatgcctg caaggcttca gcctggattt gtttttcctg
    18661 ctcggcagat aagccacaag ggcacaagta ctcggtgtca tcacgcaggt acttggcttc
    18721 atagtcgtag tattcgtttt tcggcacgat acgcacgatg ggcagtgccg tgtcacccag
    18781 aatgccgacg gtatactcgc caccaccgac aaactgctcg gcaatcacca gggggtctga
    18841 ctgcttagcc aaggtgtagg cagctgccag gtcaccggcc tgcttcactt tgctcacacc
    18901 gatgctggaa ccttcattgg cgggtttcac gaacagtggc agtcccaggg cggtgacgac
    18961 attttcgaca tgtgaactgt catccatgag cacatagtta ggcgtggtca cgccagcagc
    19021 cgtccacaat aatttggtac gccatttatc catgcccaat gcggaagcca tcacgccact
    19081 gccggtataa gggatatcca tgagttccaa tgcgccctgg atagtgccgt cttcgccaaa
    19141 acggccatgc aatgcaataa aagcccggtc atacgccttc agcgcagaca gctcatgtgt
    19201 agccggatcg aatgcatgcg catcgacgcc ctggcgttgc aacgccgcca acacggcaga
    19261 accgctctta agcgacactt cacgctcgcc agaacgacca ccaaacaaca ctgcgacttt
    19321 gccaaaagtt tgctgatatg cactcatgct gttaaaacac tttctccaat aacttttact
    19381 tcttgcacca atgccacgcc ctgttttgta cgcactgtcg ttttgacatg ctcaatcaga
    19441 gcttcaatat ctgaggcact ggcatgcccc agattcacaa taaagttggc atgcttttct
    19501 gatacctgcg ccccaccgat ggtggtgcct tttaagcctg aggcttcaat caagcgggca
    19561 gcaaagtcac cttcaggatt cctgaaggtt gagccagcgc taggcatatt cagtggctgg
    19621 ctggccaatc gcttggccag cagctgttta atctgctcgg ctgacgcttt gccatctccc
    19681 gcaggcacag taaaccagcc gccaagaaac cactcatcgg ctgccggatg ctgcacatgg
    19741 cgataagttg gttggtattc tgcccgcgca cgcacttgca ccgcgccggc acggttaatc
    19801 gttttcacct cactgaccca ctgccaggtt tcaccaccat aacaaccggc gttcatcgcg
    19861 agcgcgccgc ccacggtgcc gggaatgcct gccatgaatt cggcacctac ccggttttga
    19921 ctggctgcaa atcttgccaa cttggcacac gtcacgccag catcggcata aatacgctca
    19981 ccttgcattt ccaatgcctg caaggcctga tgcatcacaa ccaccgtgcc gcgcacacca
    20041 ccatcacgta ccagtaggtt gctacctagg cctataaacg tcagcggctc actggcggac
    20101 aattgctgca gaaactgctg caactgttcc acgctatcgg ccagcaccag acgatcggcc
    20161 ggcccgccca ctcgccagct ggtatagcgg gctaacggct cgtcatgcag cacttgtaaa
    20221 ccagtcgttt ttaagcctga agcattcatg cgctagctag ctccctggtt ttagctgcca
    20281 cgctgccaat attgccggcg cccattacaa cgacgacatc accaggctgc acgctatcca
    20341 gaatggtggc gggtaactca tccgtggttt caacaaagcg tggttcaacc ttgccattga
    20401 cgcggatggc acggatcagg ctgcgcgtat ccgccgccac aatcggcgct tcgccagcgg
    20461 catacacttc cgtcagcaac agggcatcaa ccgtagaaag cactttcacg aaatcctcaa
    20521 agcaatcacg cgtacgcgta taacgatgtg gctggaacgc cagcaccaga cggcgacctg
    20581 gaaatgcgcc gcgcgccgcg gcaatcactg cctgcatttc aatcgggtgg tggccgtaat
    20641 catccaccag cgtgaaatgc ccaccattga ccgggatttc accgtaacgc tcaaaacgac
    20701 ggcccacgcc acggaattca gccaaggctt tgaggatggc ggcatccggc acattgagct
    20761 cagtggcaat cgcaatcgcg gccagggcat tcagcacgta atgatggcct ggcaaattca
    20821 aggtgatttc gttacgcagg gtcttgccgt ttttgcgcac cagcgtgaaa tgcatcttgc
    20881 cgtgatccgc cacaatgttt tcagcacgga tactggcttc ttcatgtgtg ccataggtcg
    20941 tcactggccg ggtgacacgt ggcagaattt cacgcacatt ggcatcgtct atgcacatca
    21001 cggccatgcc ccagaacggg atctgctgca aaaattcaac gaacgccgtt ttgagcttat
    21061 caaaactgtg ctcgtaggta tccatgtgat cctggtcgat attggtcacc acagccagga
    21121 taggggtcag gtgcaggaaa gacgcatcag actcatcggc ttcgaccacg atccattcgc
    21181 cggtacccag gcgcgcattg gcactggcgg cttccagctt gccgccaatc acaaaagtgg
    21241 gatccatctc ggcctcagcc aggatgcttg ctatcaggct ggtagtcgtg gttttaccgt
    21301 gtgtgcctgc cactgcaata ccctgcttga aacgcatcag ctcagccagc atcacagcac
    21361 gtggaatgac tggcacttta cgtgcacggg cggcgatgat ttcagggttt tcttcattaa
    21421 tcgcactaga aaccaccacg acatctgcgt cactcaaata ctcggaagcg tgtccctggt
    21481 aaaccgtggc accaaaacct tgcaggcgct tggtggtcgc gttgctggcc aggtcagacc
    21541 cactcacctc aaaaccaagg ttgagcaaca cttcggcaat gccactcatg cctgagccgc
    21601 ctatgccgat aaagtgaatc tttttaactt tatgtttcat actgaacctg gtttcccgtt
    21661 tgtctttgcg atcgccagct gctggcaaat ctctgcaacc tgttgcgtag cttgtggcct
    21721 tgccaaggct cttgctttgc ttgccatctc caagcaagtg ttgcgatcca gcgttttaag
    21781 cgtatttgcc agttgtgtga catccaggtc acgctgctga atcagcaatg ccgcccctgc
    21841 ctgctgcaaa taagccgcat tcgttgtctg gtggtcatct accgcaaacg ggaatggcac
    21901 caggatgctg gcagcgccta cattggccag ttctgccacc gtcaatgcac ccgcgcggca
    21961 aatcacaaga tcagcccagg catagacttc ggccatgtca tggataaatg cacggcaatc
    22021 tgcggccaca cccgcttctg cataattctt tttaagtgct tcaatctgct tctcaccggc
    22081 ctggtgaatg atctgcggac gcgcattgac aggcaattgt gcaaacgcct gcgggagcaa
    22141 ggtattcaac gccacggcgc ccaggctgcc cccaacaact aacacccgta acacgccatg
    22201 atgttcggca aaacgctgct ccgggactgg taacgtcgta atcacttcgc gcaccggatt
    22261 accgaccatc tcgcctttgg tgcccaatgc attggggaaa ccagtcaata cacggctcgc
    22321 aatgcgggcc aacactttat tggtcagccc ggcaatggag ttttgctcgt gaatcaccaa
    22381 tggcactcct gccaatctgg ccacgacacc gcctggaaac gcagcaaaac cacccatgcc
    22441 caacaccaca tcaggccgtt gctgtttaat ggcctccctg gcttgtgcaa atgcgcgggc
    22501 cagtttcacc ggcaaagtca gccagcccat cagacctttg ccgcgcacgc cttgcatggt
    22561 catcatggct ttgtcatagg gcttgtcggc aatcaggcga ttttccatac cgccttcagt
    22621 ggcgagccag acaattttcc agccttgcgc atgcaacgcg tcggccacgg cgatagcagg
    22681 atacacatgc cctcctgtgc cgcctgccat caccatcaat gtgtatgcac ggctcatcag
    22741 ttcaagcctt tctgcaaacg acggttttca aaatcaatac gcaataacac ggacagcgca
    22801 atgcaattgg ccaaaatgcc actaccaccg taagacagta gtggcaaagt caggcctttg
    22861 gtcggcaaca ggcccatatt tacacccata ttgataaagc cctgtacgcc catccacacg
    22921 ccgatgcctt gtgcgagtaa cgctgagtaa taacgctcat tggccaccgc ttccttggca
    22981 atactgaagg cgcggatcac cagccaggca aacagcaata ccacaactag aacccccaca
    23041 tagcccaact cttcggccac cacggccagc aggaaatcgg tatgtgcttc tggcaggtac
    23101 aacagctttt ctacgctgcc acccaagccc acaccccaga actcaccgcg accaaacgcg
    23161 atcaaggcat gagacagctg gtaaccggta tcaaacgggt catcaaatgg atttaaaaag
    23221 cctttcatgc gttccaagcg ataaggctcc atgaccacta ggccaatcac tgccaatggc
    23281 agggcaccga gcatcgccaa gaacactttc aggttgacgc cacctagcca catgatggaa
    23341 aacgcaatcg atgcagtcac ggcaaaagca ccaaaatccg gttctttaag cagcaaaaca
    23401 cctacaaaca ccatcacgcc tagcatcggc aagaagcctt ttttaaagct gtgcatattg
    23461 ctggccttgc gcacggcata gtccgaggca tacatggcaa taaataactt catgagttct
    23521 gaaggttgca gattcatgat cagcagtgaa atccagcggc ggctaccatt caccaccttg
    23581 ccaatgcctg ggatcaatac caggattagc agcacaatgc ccataataaa caggtaaggc
    23641 gacatttttt gccaccaggc catcggtatc tggaatacga tcacggcagc caccaggcca
    23701 acgacgatat acaaggcctg acgtaccagg aagtaagtac tgttgtgccc gaacatcttg
    23761 ctggaatcag cataggcaat tgaggctgaa tagaccatca ccatgccgaa cgccagcaag
    23821 gacagcacca cccacaataa cgacacatcg tagttaggag agttgtagcg ctcgcgattc
    23881 atcatgagtg gcccaagcat gcgacctcca tggctttcac cgcttccaca aatacttgcg
    23941 ctctatgttc gtaattgcgg aacatatcga aactggcaca cgctggtgac aacagcactg
    24001 cgtccccttc ttcggccagg gttttggcaa tgtgaaccgc tgcggtcaaa tccaccgcat
    24061 ccacgaccgg caattgcacg ttgtgcaaaa tttgctggat tttgggtcca tcgcgaccaa
    24121 tcagcacgac ggcccttgca tggtgcaata ccgcttcgcg caaggggctg aaatcctggc
    24181 ctttaccatc cccgccagca atcagtacca ctttttgtgg tttcccctgt ttgagcatgc
    24241 cactgatggc cgcgcaagtc gcaccgacat tggtcccttt agagtcgtcg tagaagtcaa
    24301 cgtcatcaat atttgctacc cactctacac ggtgcggcaa cccactaaag gactgcacgg
    24361 tatgcaggat agagaccttg tctatcccaa tagactgggt cagggcaatc gccgccagtg
    24421 cattggcgat attgtgtttg cctcgaatct gcaaactgtc tgcttcaata tagtgtcggt
    24481 tacctgcgca taaccagccc gcatcattca gaccgtaatc gttgatggtc ggggaaggat
    24541 tcacgccaaa cgacacctgt ggcaactggc cagcaagtgc agcactaggc gcgtcatcac
    24601 gattcaccac tgcaatgcgg gcctgctggt aaatacgggc tttggcatgc gcataatcct
    24661 ccatgccatc gtaccggtcc atatgatctt cagacaggtt caggatagtg gctgcgtctg
    24721 cctgtaggct ataggtcgtc tccagctgga aactggaaag ctcgagtaca tagacatcag
    24781 gcgcagtcat ttccaaggtg tccagcacgg gcaggccaat attgccggcg acaatggtat
    24841 tcaaccctgc ctgcttgcag atctcgccga ccaatgaggt caccgtggtt ttaccattgg
    24901 accctgtgat cgcaatcact ttggcatacg gtgcacggaa acgggcaaac agctccacgt
    24961 caccaaccac gcttttacct tgcgccctca gttcgacaat ggcaggctct ctcaaagaaa
    25021 cacccgggct tgccacaatc aggtccatgg cagccaataa agaggcattg aacgcgccaa
    25081 gatggtactc cacatcggga aactcttgat gcagacgatc caggccagac ggatgcaggc
    25141 gtgaatcaaa catcaccact tccgcgccca tctggcgcag ccagcgcaat gccgagaatc
    25201 cggtttcacc cagtcccagt actgcaatac gctgttgttc gaagtgtttt ttcatgcttt
    25261 cgcctgacgt ttgacccagt gtcttatctc aatttcaagg tggacaaccc aaccagtacc
    25321 agcatcatgg taataatcca gaaacgaacc acgacttgcg tttctttcca gcccttttgc
    25381 tcatagtggt gatgcaaggg tgccatgagg aaaatgcgtt taccggtgcc agtcattttc
    25441 ttggtgtatt tgaaatacag cacttgcacc gcgaccgaga aggtttccac cacaaatacg
    25501 ccacccatga tgaataacac gatttcctgg cgcactatca ccgcgaccac acccaacgca
    25561 gcgccgagtg ccagcgcacc aacatccccc ataaatactt cggccggata agcgttaaac
    25621 caaagaaagc caagcccggc acctgccatg gctgcacaaa acaccatcaa ctcacttgca
    25681 ccagccacat aaggaatgcc caggtatttg gaaaatacgg catggccggc cacgtaagaa
    25741 aagatagcca gtgcgctccc caccagcacg gttggcagcg ttgccaggcc atcaaggccg
    25801 tccgtcaggt tgactgcatt actgctaccg acaatcacca ggtaagtcag cacaatgaaa
    25861 ccgaccgcac cgagcgggaa caccagatgt ttgaagaaag gcacgatgag cgtggtttct
    25921 tccggcgtgg ttgctgtggc atacaggaaa cccgcaacgc caaaaccgat caggctttgc
    25981 cagaaatact tctctttggc agacaatcct ttgggatttt tgtagaccac tttgcggtag
    26041 tcatctaccc agcccacggc gccaaagcct agcgtggtaa tcagaactac ccagataaaa
    26101 cggttatgca ggttgcccca cagcaaggtt gcaatcgcaa tcgccaccag gatcagcgca
    26161 ccgcccatgg tcggtgtacc cgccttaacc agatgcgttt gcggaccatc atcacgcaca
    26221 gcctggccaa ccttgtattc agtcagcttg cggatcatgg ccgggccaac cataaatgaa
    26281 atcgccagtg ccgtcagtgt cgcgagcaca gcgcggaagg tgatgtaatt aaatacatgg
    26341 aatccatgta catcagctgc cagccatttt gccaattcaa gtaacataag cttccctatt
    26401 tccccactaa ctcagtggcc tgtgattcca ccaaggcatt gacgacccgc tccatttgca
    26461 tgaaccgtga ccccttcacc aataccacat cgtttgttgt tgtttcatgc tgcaaagccg
    26521 ccaccaaagc ctcgagcgag gcaaaatgct gtgcttgtgc cccaaaggct tgcacggctg
    26581 cttgcgttaa cttgcccaag gcatacatcc tggcgatgcc actctcttta gcgtaacgac
    26641 cgacctctgc gtgcatggcc tccgcatccg tacctaactc gcccatatcg cccatcacaa
    26701 atacggtatt tgcaccactg cttttcaata catccagggc aaccttcatg gagtccggat
    26761 tggcgttata agtatcgtca atcaacctcg caccattggc tgccactttt tgctgcaaac
    26821 ggcctttcac accggcaaag ccttgtaacc cttgcgcaac ttccgccagg ctggcgccag
    26881 ctgccaaact ggccgccgcc gcggccaatg cattcatgat gttgtgtgcg ccgggtacca
    26941 ccagcgtcaa tgtcacggcg tcgccctggt aatgcagttc aaaagtggac tctgcatccg
    27001 tgatttcgcc acgcacatta gccgctggat gcaaaccaaa tgtgagcacg cgacgttctt
    27061 tatttaatcc gcgccagtag tctgcaaatt cgctatccgc attgatgact gcaatcccgt
    27121 catgactcaa tccagcaaaa atctcgcctt tggctttggc aatgttttcg cgtgagccaa
    27181 gttcaccgat atgagcggta cctgcgttat taatgaccgc cacatcaggg cgggccaagc
    27241 gcgtcaggta gtcaatctcg cccaggtgat tcatgcccat ttcaatcact gcacaacggt
    27301 gagcaggccg caaccgtaac aaggtaagcg gcatgccaat gtcattattg aagttacccg
    27361 ctgttgccaa cacattgtct gcactaccca catgcgcctg catgatggca gcgatcattt
    27421 ccttggtggt tgttttgcca ttactgcccg tgacggcgat taatggcaaa gccagttgct
    27481 gacgccacca actggataac tgacccaatg ccaggcgtgt atcttttacc aataccgtcg
    27541 gcaagtgcgt ttcaactggc ttactgacca gcgcggccac cgctccttgc gcagccacct
    27601 ggtccaggaa atcgtgacca tcaaactttt cccctgccaa agcgacaaat aattgtcctg
    27661 gcttagcacg acgactatca gtgtcgactg aagtcaccac ggcgtcgcca ccttgcaaag
    27721 tgccttttaa tacgtcagca atggtgtgca gtgaaatcat gcttgcacct tttgctttaa
    27781 tgcgtgttga ttgggtacgc tctttttcaa tgcgttttgc acccactcgg catcactgaa
    27841 tggataacga atcccctgga tttcctggta atcttcatgc cccttgccag ccaccaacac
    27901 cacatcccct ttactggcct gactgacggc aagcgcgatg gcttttgccc ggtccacctc
    27961 tacattgact tcacgcatca tgccttcagc aatggcagcg ataatggcat aaggattttc
    28021 ggtacgtgga ttatcattgg tcacaatgac tgtatccgcc agctcatcgg caattcgccc
    28081 catttcactg cgtttgccac tgtcacggtc accgccacag ccgaatacgc agagcaattt
    28141 gccacgggcc tgaacacgta aggtatgcag tgctttctcc aatgcatccg gcgtgtgtgc
    28201 gtaatccacc accaccaatg gcaaatcgcc cccgccaaac atctgcatgc gcccagcgac
    28261 cggcaccagg ccagagacag cctgcaaggc agcaggcaaa gccacctcat tggcaagcag
    28321 gcatgccaat accgccaacg cgttatagac gttaaactgc cctaaggcat gcagctggat
    28381 atcgccgcgg ccttgcgccg tatgtacctg gatttcaaac ccagtcgcat gcatggtgac
    28441 tgccgtggca caaacatcag cctcctgatg caagccataa ctcagcacct ttttgccctg
    28501 tttgcgcaat gcatcaatca aatccaggcc aaacgtgtca tctgcgttca ccacagcagc
    28561 agagagtgtt tgccagtcga acaatttacg cttggcagcc tggtaagctt ccatggtgag
    28621 gtggtagtca agatgatcgc gcgtcaaatt cgtaaacacc gcgacatcaa atgccacccc
    28681 attaacacgt ccctggtcca ggccatgtga agacacttcc atagcaacca tgcgaacgtc
    28741 atccaacaca aatgtggcca gcattctttg caactccacg gggccgggag tcgtattttg
    28801 cgttggttgc aaatcctcga ttgggccatt acccaaggtt ccaatcactg cggacttctg
    28861 ttgcaggaaa cgataagcct gtgccagcca atgcgtcacc gtggttttgc cattagtgcc
    28921 cgtgaccccc acgcaccaca attgctcgga tgggtcttta taaaactggc tggcgatgtg
    28981 cccgacttgt gacttgaggt ccgcaatagc gatgttatgc acggtccact caggattcca
    29041 gtcaaaaccc tcgctatccc agatcaccgt attcacgcct ttagcaatag catcctcaat
    29101 atagtcgcgg ccatcgctgt gctgacccgg gtatgcgagg aacaggccat gcttttcgac
    29161 ctggcggcta tcagccgtga tgccgtgtac tggcgctgga ataatgtact tactcacatc
    29221 gcctccttga cttcagcggc atctgccgag gtagtggccg ggaccacatt gccatcttgc
    29281 ggaatcacca gcatgcgcaa cacatcgttc atgactgcac taaagaccgg tgcagccacg
    29341 ctgccaccgt aatattcacc attactaggc tcatctatca ttaccgccat gatcaggcgt
    29401 ggattcgaag cgggcaccat accaacaaag gaacccacat atttgtcatg ctcgtaccca
    29461 cccgcaccca gcttgtgcgc agtgccggtt ttgcctgcca cccgataccc cgtgacctgc
    29521 gctttcaatg ccgtcccccc tggctgcacc accagctcca gcatgtcttt cactgaatta
    29581 gccaccttgg ctgaaaacac ctggcggccc acaggcggct cagacagttt ggtcaaggtc
    29641 acaggcaata actcaccttc attggcaaac acagtgtaag cgcgcgccag ctgcaacaag
    29701 gtcacgctga tgccgtgacc ataagacatg gtggcctgct cgataggacg ccaggtcttg
    29761 taatcgcgaa ccttgcccgc cgcttcacca gggaaaccaa ttttagttgg tgtcccaaag
    29821 cccaattgca cataggtatt ccacaactgc tcgcgattca gatcgagcgc cattttggcg
    29881 gagccaacat tagaagactt ctgaatcact tgcgacacgc tgagcacctg gttagggtgt
    29941 gcatcatgta tggttgcagt gcctatacgc aggtaacccg gcgcagtctg gattttggta
    30001 tccggctggt atgggccaaa ctccatcgca gcagcggcag tcaccggctt catcgtcgag
    30061 cccggttcaa aaatattgac gatggcggag ttacgcagct tgctggcgac attgaccgga
    30121 ttattggggt tataggtcgg aatattcacc agcgccagta cctcaccagt ctgggcatcc
    30181 agcaccactg ctgcagccgc tttggcttta tgtttttcca ctgcgcgaga caattcacgg
    30241 tggaccacgt attgcacagt acggtctatg ctcaatacga gatcctggcc atcgtgcggc
    30301 aacttgaccg ccaccaggtc ttcaaccaca tgccccttac gatcacgcac aaagtcacgc
    30361 ttgcctggcg tgccagacaa cacattgttg cgatataact ccatgccctc aacaccggta
    30421 tcatccacac cggtgaaacc gacgatatgc gccgccactt ctgccgccgg gtagaagcgt
    30481 ttgtattcct tctggctgaa tacaccgggc actttcatcg ccatcacctg ttttgccaca
    30541 tcgggcgcca ctctccgttt gatatacaca aattcttttt tcttttgctg cactttctgc
    30601 tgcaactctc ttgctggcat ccccagcaat ttggccattt gtttcaactg caccgccgag
    30661 atttgcacat cggtcgggtt cacccacacc gactccaccg gcaaactgat cgccaatggt
    30721 ttgaagttac ggtcataaat cttgccgcga tgtggcatca acaccacttt gcgccgtgaa
    30781 aacgcctctc cctttttaat caggtaatcc ttatgcaagg tttgcaggta aaacccgcgc
    30841 cccagcagca gggcaaagct caacagcaac aacaccaaca gcgttcttct acgccaggcg
    30901 ggcagcttca ccagcttgtg ttgactctct ttcagcaaca tgaccattaa cctgcaacca
    30961 ttcctgctta tggtgccgca ggcacatcta tcataatcac ttgggtctgc tgcatgctcg
    31021 ggctatgcat gtgcaaacgc gtggttgcaa aatcttccaa ccgcgaatgc atggcccagg
    31081 tactttgctc aatctgtaac tggtcgtact ccgtcatgta ctgcttggtc aacacttcct
    31141 gcctgtccaa ctcaaaatac aatttgcgcg ccttgtattg cgcggtcacc acgcccaacg
    31201 ccatggcaat cgtcaatgca aacaaaatca aattcaggcg caacatgacc caaccctatt
    31261 ccgcccatta ctgcacatga gtacgctcag cgacacgcag caccgcgctg cgcgaccgca
    31321 cattggccgt cacttccgcc ttaccgggct tgatcgcccg gccaacagaa accaggcgcg
    31381 gctggggcag gtccttggcg cggaccggca accccgcggg caggttatcc ctgtcttctt
    31441 cagactggat aaatcgcttg acgatacggt cctccagcga atgaaagctg atcactgcca
    31501 gccgcccttg cggacacagc aattttaagc aatcaggcaa cactaacgag agttcctcaa
    31561 gctcttgatt gacgaaaatc cgtagagcct gaaatgtgcg cgttgcaggg ttctggcccg
    31621 gctcattctt ggggatggcg cctgccacga ccttggcaag ttgccctgta gtggtgatgg
    31681 cacgcccatc ttcgcgctct ttaacaatcg cccttgcaac ctgcttagca aaccgttctt
    31741 caccataatc ccttatcacc tcacccaatt gtttttcaga catcgtcacc agccactctg
    31801 cggctgtttt accccgactt tgatccatac gcatatccag tggcccgtca aaacggaaac
    31861 tgaatccacg cacaccctca tcaatttgcg gcgacgaaat acccagatcg agcaatattc
    31921 catctaccgc ttgcacaccc agccccacca acactgttgc catcgctgaa aaatgcgcat
    31981 gcaccatcgt aaaacgcgca tcggcgattg tttgcccatg cgccacggcc gccagatcgc
    32041 gatccaagcc gatcagtcgt cctttgctac ctaatttgga aagaataaga cggctgtgcc
    32101 caccacgccc gaaggtacca tccacataca caccgtcagc ctgcacattc aaagcatcta
    32161 ccgcttcatg caataacacg gtgatatgac tggcggcgac tggtgacact gacgtttccg
    32221 tcaaggacgc gggcgctgac ttcgcgcccc gcgtcatagc gagaatcctt ctagctcttg
    32281 cggtatctgc agctggtcgg tcgccatcag gctttccagc tgctgatccc atgcagcaac
    32341 atcccacaac tcaaaatgac taccttgccc gaccatcatc acgtctttat cgagcttggc
    32401 aaacttgcgc agtacggcat tcaccagcaa acgaccggca gaatccagat gcatcacatc
    32461 cgcctggccc acgatcatgc gttgaatacc actggtttga ggattaaaac tggacaaact
    32521 ggtcagcttg gtttcaatcg gctcccaagc ggcttgcgga tacaggatca ggcagcgatg
    32581 tggatgcgct gtcagcacca cttcaccttg cccgcctgcg gtcagggcgt cacgatgctt
    32641 ggccggcaca ctgagccgtc cctttgcatc caaacttaac tgtaccgccc cgcgaaacat
    32701 gaataattac cttagccaac ccgttttgat taaaaatgga atgaatttcc cacaattcaa
    32761 cacaaaatca ctcttttgca cactatagat aaaaaaaaag tgcttggc
//
LOCUS       DATMSK010000208         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_226899,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000208 DATMSK010000000
VERSION     DATMSK010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226899"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VNZ84_07020"
     CDS             complement(<1..333)
                     /locus_tag="VNZ84_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HWU66748.1"
                     /translation="MALHEMDAVAEETSMSATRLWTTRLLVALLVLAVLAGIGYGIKK
                     LFSGGATQKKQITTVKLLPDTPPPPPPPPPKEPPKETPKEQPKEAPKEPEPKPAEAPP
                     AENLKMEGP"
     gene            complement(336..740)
                     /locus_tag="VNZ84_07025"
     CDS             complement(336..740)
                     /locus_tag="VNZ84_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWU66749.1"
                     /translation="MQVQNDAQPYDTINITPMLDLAYVLLVIFILMTTAGVQGLTMNL
                     PKPSNKPSTEKHDIKIVQVQQGGTLTINGVGVSMAELETQLNQAKAMDPKFNVMIKGQ
                     PQAPYAGVIGVIDLVNRLQIENVGLVTGKIGT"
     gene            complement(744..1154)
                     /locus_tag="VNZ84_07030"
     CDS             complement(744..1154)
                     /locus_tag="VNZ84_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWU66750.1"
                     /translation="MSEGVKEENQLYDEINITPMLDLAYVLLVIFIIMTTASVQGVKV
                     DMPHTVNSAALAKPQMRAITVTSDGSVYLDAFPVDMAQLEQRLAEYKSSNPDLPVVLK
                     GDAAAHYEKVSEVLEICKRLDITEVGLVTKKVVE"
     gene            complement(1165..>2816)
                     /locus_tag="VNZ84_07035"
     CDS             complement(1165..>2816)
                     /locus_tag="VNZ84_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWU66751.1"
                     /translation="GLLGLSLSTSVFAAWNKDWATQQKITIDTANIKEAVNQAPVLVR
                     LHSGNFDFTGANVDGADLRFIAADDKTELKFSLEKYDAVNELAVAWVVLPQVSAANKD
                     LSISLYTGNEGAKSASDVQALNDSALLARFAFADNALLKDSGPAQLTASGNAVVQKAG
                     LIAESATLNNSPLTIALPAEPVLANGMTWSVWLKPVNLPQTAEIFKSGNVKLTLEGSA
                     LVLHAGAASTQAGELKPGQWQHLAVVFQAGQAIVYVNGQVAGQVEASDAQANAEFTLG
                     QNLNAEVDELQLASTARSAAWLALVAKSQGADASMVKVIAAEGDEDGEEGEANYMGIL
                     IDSLTVDAKVVIGILAVMFAISAWVMVSKARLVTRTDKDNALFLKRFQQSNTGDLLSL
                     DKTGTYTHSSLFGLYKAGLREIKKREVDGKVALGGTSIDAIKAAVDADLVRETQRMNS
                     MMVLLTIAISGGPFLGLLGTVVGVMITFAAIAAAGDVNVNAIAPGIAAALLATVAGLG
                     VAIPALFGYNYLASRVKNITIAMQIFVDEFITRTAELYGKE"
BASE COUNT          631 a          805 c          690 g          690 t
ORIGIN      
        1 agggccctcc atcttcaagt tctctgcagg cggcgcttca gcgggttttg gctcgggctc
       61 tttgggggcc tcttttggct gctccttggg cgtttccttg ggtggctctt taggcggcgg
      121 aggcggtggg ggcggcggtg tatccggcag caatttcacc gtcgtaattt gttttttttg
      181 tgtggcacca ccagaaaaca gttttttgat gccataaccg atgcctgcca ataccgccag
      241 caccagcaac gcaaccaata ggcgtgttgt ccataaacgt gtggcagaca tagacgtttc
      301 ttctgccacg gcatccattt catgcaatgc catgcttatg tcccgatctt gccggtcacc
      361 aggccgacat tttcaatttg cagccgattg accaggtcaa tcacgccgat tacaccagca
      421 tagggcgctt gcggctggcc tttgatcatc acattgaact tcgggtccat cgctttggcc
      481 tggttaagct gtgtttccag ttcggccatg ctcacgccaa ccccattgat ggtgagtgtg
      541 ccgccctgct gcacctgtac gattttgata tcgtgttttt cagtacttgg cttattggat
      601 ggcttgggca agttcatggt caggccttgc acaccggccg tcgtcatcag gataaaaatc
      661 accagcagca cataggccag gtccagcatg ggcgtaatat tgatggtgtc gtaaggttgg
      721 gcgtcgtttt gtacctgcat ggtttactcc accactttct tcgtcactag accaacttcg
      781 gtgatatcca gccgcttaca gatttccagc acttcagaca ccttttcata atgcgccgcc
      841 gcatccccct taagcaccac aggcaagtcg gggttactgc ttttatattc agccaaccgt
      901 tgctccagct gcgccatatc taccggaaac gcgtctaggt agacgctgcc atcactggtc
      961 acggtaattg cacgcatttg cggcttggcc agtgcggcag agttcacggt gtgtggcatg
     1021 tcgaccttaa cgccttgcac ggatgcggtc gtcatgatga taaaaatcac cagcagtacg
     1081 tacgccaggt ccagcatggg cgtaatattg atctcgtcgt agagctggtt ctcttctttg
     1141 acgccttctg acatcgccta aaccttattc cttaccgtac agctcagcag tacgggtaat
     1201 gaattcatcg acaaatatct gcatggcgat ggtgatgttt ttgacacggc tggccaaata
     1261 gttgtaacca aacaaggcag gaatcgcgac gcccagacca gccactgtag ctagcaacgc
     1321 cgccgcaata cccggtgcaa tggcgttaac gttgacatca cccgccgcgg caatcgcagc
     1381 aaaggtaatc atcacaccga ccacagtccc tagcaggccc aggaaaggac caccggaaat
     1441 cgcaatggtg agcaatacca tcatgctgtt catgcgctgg gtttcacgga ccaggtcagc
     1501 atccactgcc gccttgatcg catcaatcga ggtgccacct aaagccactt tgccatccac
     1561 ctcacgcttt ttgatctcgc gtaaaccggc tttgtacaag ccaaacaggc tggagtgcgt
     1621 ataggtcccc gttttgtcca gggacaacag gtcgccagta ttagattgct ggaagcgctt
     1681 gagaaacagc gcattgtcct tatcagtacg ggtgaccaga cgcgccttgc tgaccatcac
     1741 ccaggcactg atagcaaaca tcacagccag gatgccaatc accaccttgg catccacagt
     1801 cagactgtcg atcaggatgc ccatatagtt ggcttcgccc tcttcgccgt cttcgtcacc
     1861 ctctgcagca atgactttga ccatgctggc atccgcccct tgggatttag ccaccagtgc
     1921 cagccaggcg gcactgcgtg ccgtggaagc cagttgcagc tcatcaacct ccgcattcaa
     1981 attttgcccc agagtgaatt ccgcgttcgc ctgcgcatcg ctggcttcca cttgaccagc
     2041 cacttgacca ttgacgtaaa caatggcctg acccgcctgg aagaccacag caagatgctg
     2101 ccactggcct ggcttcaatt caccagcttg agtgctggcg gcacctgcat gaagcaccaa
     2161 ggcgctgccc tctagtgtta acttgacgtt accacttttg aaaatctcag cagtttgtgg
     2221 caaattgacc ggcttcagcc atacagacca agtcatgcca ttggccaaga ctggttcagc
     2281 aggcaaggca atcgttaacg ggctattgtt taaagtggcg ctttcggcaa tcaagcccgc
     2341 tttttgcacc acagcattgc cgcttgcggt caattgagca ggaccactgt ctttgagcaa
     2401 ggcattatcg gcaaaagcaa agcgggccag taaggctgaa tcattcaggg cttgaacatc
     2461 gctagctgat ttagcgcctt cattcccagt atacaaactg atagacaagt ccttattggc
     2521 agcagacacc tgtggcagca cgacccaagc cacggccagc tcattcaccg cgtcgtactt
     2581 ttccagtgaa aatttaagtt ctgttttatc atctgcagca ataaagcgca ggtcagcacc
     2641 atcaacatta gccccggtga aatcaaagtt gccagaatgc aggcgcacca gcaccggcgc
     2701 ctggttcaca gcctccttga tattggcggt gtctatcgta attttctgtt gtgtcgccca
     2761 gtctttgttc caggcggcaa acacggatgt ggacaggctg agccctaata atccaa
//
LOCUS       DATMSK010000209         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_15747,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000209 DATMSK010000000
VERSION     DATMSK010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15747"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /gene="asd"
                     /locus_tag="VNZ84_07040"
     CDS             <1..492
                     /gene="asd"
                     /locus_tag="VNZ84_07040"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441436.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HWU66752.1"
                     /translation="NQALADEFQDGHFATLYLSPRDYHRIHMPCDGHLLRMTYVPGDL
                     FSVNPVTAQGVPGLFARNERVVCEFDSAHGKFVLVLVGATIVGSMATTWHGVVNPPRP
                     GNISVWEYAEHQIHLKQNEEMGRFLLGSTVVLLFPKQPLQFNPVWQEGRPIRLGEAMA
                     NRG"
     gene            complement(520..1041)
                     /locus_tag="VNZ84_07045"
     CDS             complement(520..1041)
                     /locus_tag="VNZ84_07045"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HWU66753.1"
                     /translation="MIDRDGYRPNVGIILCNARNEVFWGKRIREHSWQFPQGGIKHGE
                     TPEQAMYRELMEEVGLRPEHVKILGRTRDWLRYEVPTNWIKREWRGSYRGQKQIWFLL
                     RMVGRDSDVSLRASTHPEFDAWRWSEYWVPMESVIEFKREVYRLALNELAAHLSGDTR
                     KKPRNHSSSIIAS"
     gene            1284..2240
                     /locus_tag="VNZ84_07050"
     CDS             1284..2240
                     /locus_tag="VNZ84_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWU66754.1"
                     /translation="MKPLLILLAAFSSCIAWSCQAGGFAYISNQGNNTVSVIDLASNQ
                     VIASIEVGKAPVGVATSAALGRTYITNVDGQSISVIDSQHYKVLQELPLHGSPVGIAL
                     SPDSKTLFVADWFEDKILALDTADLTQQRTVNVGKAPAGIIVSPDGKQLYVANRDSND
                     VAIIDTASMQIVKRIAVGEHPFGLGLSRDGKQLYAVNVYSNSVSMIDTQTHQVNTIKV
                     GEHPYCVTVSTDGSRIYVTNTQSDNVSVIDVAQQKVISIVPVGGYPEGISFDHANQRV
                     YVANWFDNTVSVIDASNNKLVQTIATDKQSRAFGQFIQETAH"
     gene            2303..2824
                     /locus_tag="VNZ84_07055"
     CDS             2303..2824
                     /locus_tag="VNZ84_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWU66755.1"
                     /translation="MKKLLALIALAVIPLTATAHGPSPQKVEKSVTIKADPAKVWALV
                     KDFGNLQKWHPAVASTKLETKDGETYRLLTLKGGGTIYEKLRSIDDADMKLKYEIVEG
                     VLPVADYNSFITVKAGPGAGESTVTWVGRFYRVYKLNPPIPPGQDDESAINAVTGVYD
                     AGLANLKKVAEGN"
     gene            3020..3451
                     /locus_tag="VNZ84_07060"
     CDS             3020..3451
                     /locus_tag="VNZ84_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HWU66756.1"
                     /translation="MKNSQQGFTLIELMIVVAIIGILAAVAIPSYQDYTVRARVTEGL
                     NLASAAKSTVAENITNGGGTSIPATGNCRGVNTFTTLTGHTQSLACTDGTGALVATMD
                     TTAKGVAITLTPATSERGVTWTCTTTEANFKYVPAECRNAG"
BASE COUNT          867 a          964 c          873 g          771 t
ORIGIN      
        1 aatcaggcac tggcagatga atttcaggat ggccactttg ccaccctcta cctcagcccc
       61 agagactatc accgcatcca catgccctgc gatgggcatc tactgcgcat gacctacgtc
      121 ccaggcgatc tgttttcggt caacccggtg actgcgcaag gcgtacctgg cctgtttgca
      181 cgcaacgagc gcgtggtgtg cgagtttgac tcagcgcatg gcaagtttgt gctggtgttg
      241 gtaggcgcca ccatcgtcgg cagcatggcc accacctggc atggcgtggt caatccgcca
      301 cgtccgggca atatttcggt atgggaatat gccgagcacc agattcacct caagcagaat
      361 gaggaaatgg gtcggttttt attgggctcg acggtggtgc tgctttttcc aaaacagccc
      421 ttgcagttta atccggtatg gcaggaaggt cgcccgatcc ggctgggcga agccatggca
      481 aacaggggct agcacgcatc aaccccacgc aagccttgct caggaggcaa taatggaact
      541 gctgtgattg cgcggttttt tgcgcgtatc cccgctgagg tgagccgcaa gctcattcag
      601 ggcaagacga tacacctcgc gcttgaactc aatcacactc tccataggca cccaatactc
      661 gctccagcgc caggcatcaa actcaggatg cgtgctggca cgcagtgaca catccgaatc
      721 gcgacctacc atacgcagca ggaaccagat ctgcttctgg ccgcgataac taccgcgcca
      781 ctcacgcttg atccagttgg tggggacttc ataccgcaac cagtcgcggg tgcggccaag
      841 aattttgacg tgctccgggc gcaagccaac ttcttccata agctcacggt acatggcctg
      901 ctcgggcgtt tcgccgtgct tgatgccacc ctgcggaaac tgccaggagt gttcgcgaat
      961 acgcttgccc caaaaaacct cattgcgggc attgcataat atgatgccga cattcgggcg
     1021 atatccatcg cgatctatca tgacgtacta ccttaataat ttagtgtaat tttttcacac
     1081 ttcagccccg attgaaagcc gcatgccaac ttgccttact ggaagccaga ggatcacgcc
     1141 tgcaggccgt gcaaattcgg gaatatcacc tgccaagcgc gccttgacgc tgtttgcaag
     1201 ccccccgtat actgcgtagt caaccatatc ggcttttcaa gcgtttgatt cacatcacaa
     1261 ccattccgca gatccgcttt ccattgaaac cgttgcttat cctccttgcc gcattctcct
     1321 cctgtatcgc atggtcatgc caggctggcg gctttgccta catcagcaat caggggaata
     1381 acacggtatc ggtaattgat cttgcgtcga atcaggtcat tgccagcata gaagttggca
     1441 aagcgcctgt cggtgtcgcg acttctgcag ccctgggccg cacctatatc accaatgtcg
     1501 acggccaatc cattagcgtg attgactctc agcattacaa agtattgcaa gagttgccat
     1561 tgcatggctc gccggtcggc attgccctct cgccagacag caaaaccctg tttgtcgccg
     1621 actggtttga agacaagata ctggcgctgg ataccgccga cctgacgcaa cagcgcacgg
     1681 tcaatgtcgg caaggcgcca gcaggcatta tcgtcagccc ggacggcaag cagctttatg
     1741 ttgccaatcg cgatagcaat gatgtcgcta tcattgatac cgccagcatg cagatcgtca
     1801 agcgtattgc cgtgggtgaa cacccatttg gcctgggcct gagccgggat ggcaagcagc
     1861 tttacgccgt taatgtttat agtaatagcg tttccatgat tgatacgcaa acacaccagg
     1921 tcaacaccat caaggtgggg gaacatccct attgcgtcac ggtcagcaca gatggcagcc
     1981 gcatttacgt caccaatacg caaagcgaca atgtctcggt gattgatgtt gcccagcaaa
     2041 aagtcatcag catcgtgccg gtgggaggtt accccgaagg catcagtttt gatcatgcga
     2101 accagcgggt atatgttgcc aactggttcg acaacacggt ttcagtgatc gacgccagca
     2161 acaataaatt ggtccagacc attgccacgg acaagcaaag ccgggcattt ggccagttta
     2221 ttcaggagac ggcgcactga gaatgaatcc agccccggat tcggatttag caccaagact
     2281 tttatcaacg gagaaaaatc gcatgaaaaa actactcgcc ctcattgccc tggccgtcat
     2341 tccattgacc gccactgcgc acggtccatc gccacaaaaa gtggaaaaat ccgttaccat
     2401 caaggctgat cccgccaagg tatgggcgct ggtgaaagac ttcggcaacc tgcaaaaatg
     2461 gcaccctgct gtggccagca ccaagctcga aaccaaagat ggcgaaactt atcgcctgct
     2521 gaccctgaag ggcggtggca ccatctacga aaaactgcgc agcattgacg acgccgacat
     2581 gaagctgaag tacgaaatcg tggaaggcgt attgcccgta gctgattaca actccttcat
     2641 taccgtcaag gcaggccctg gcgctggcga aagcaccgtg acctgggtag gccgcttcta
     2701 ccgcgtgtac aagcttaacc cacctatccc accaggtcag gatgacgaat ccgccatcaa
     2761 tgctgtgacc ggtgtgtacg acgctggcct ggccaacctg aagaaagtgg ctgaaggcaa
     2821 ctaattgcca tcagcaaacg gtgacatttt ttgtcactga aaatcaaaaa agcggatgtc
     2881 accatccgct ttttttatgc ctgcgttatg cttgattcat ctgataaacg aagtgatcaa
     2941 ccaacacccc gtttatcgac gcaacacccc tggcagacat ctgctagaga ctctcctaaa
     3001 ttgattgaaa ggtgatgaca tgaaaaactc acagcaaggt tttactctta tcgaactgat
     3061 gatcgtggtc gcgattattg gtattttggc agcggtggcg attccttcgt atcaggacta
     3121 caccgtacgt gcacgtgtaa ctgaaggctt gaacttggca tcagcggcca agagtactgt
     3181 cgcagaaaat ataacgaatg gtgggggcac atcaatacct gctaccggga attgcagagg
     3241 cgtgaataca tttaccaccc tcacaggtca cactcaatct cttgcgtgca cagatggaac
     3301 aggggcctta gtagcaacaa tggacacgac ggcaaaaggt gtagcaatca cattgactcc
     3361 agcaacaagt gaacgcggtg ttacttggac gtgcaccact actgaagcaa acttcaagta
     3421 tgttcctgct gagtgcagaa acgctggcta atgtggtaat tgtagtaaaa gcccg
//
LOCUS       DATMSK010000210         6932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_462504,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000210 DATMSK010000000
VERSION     DATMSK010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462504"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VNZ84_07065"
     CDS             complement(<1..586)
                     /locus_tag="VNZ84_07065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWU66757.1"
                     /translation="MQSLIDLTKVRESLALDPALLSLMQHWLGTFAGQAQPIKMQVLS
                     RRQLVSVMDPKVASTDPAFEALPADVYKMLLGYLDQEHRQHQCLETAIGKLHIFPLSQ
                     SIAHKNAILLIQHAALSTEAVALVEQCLQMFHYYARLIIENERDGLTGLLNRKTFDQC
                     VSKILLNDYKPEYRFPLHHYLVIFDIDFFKRINDN"
     gene            complement(728..803)
                     /locus_tag="VNZ84_07070"
     tRNA            complement(728..803)
                     /locus_tag="VNZ84_07070"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(768..770),aa:His,seq:gtg)
     gene            complement(819..895)
                     /locus_tag="VNZ84_07075"
     tRNA            complement(819..895)
                     /locus_tag="VNZ84_07075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(859..861),aa:Arg,seq:tct)
     gene            complement(934..1010)
                     /locus_tag="VNZ84_07080"
     tRNA            complement(934..1010)
                     /locus_tag="VNZ84_07080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(974..976),aa:Pro,seq:tgg)
     gene            1094..1951
                     /gene="folD"
                     /locus_tag="VNZ84_07085"
     CDS             1094..1951
                     /gene="folD"
                     /locus_tag="VNZ84_07085"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF008058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HWU66758.1"
                     /translation="MSAQIIDGKQIANQLLDDIKVNIDQRLQNGKRAPCLAVILVGDD
                     AASAIYVRNKRLACEKVGIVSVARNLPSSTSQEELFDLIRQLNADDATDGILVQSPLP
                     HHIDETEILALINPAKDVDGFHPYNIGRLAVRQPLLRSCTPFGVIKMLQASGISLKGM
                     DAVVVGVSNHVGRPMGLELLLAGCTVTSCHRHTKDLAAHIGRADLIVAAAGKAGLIKG
                     EWIKPGAVVVDIGINRLPDGKLTGDVDFAVASQRASHITPVPGGVGPMTVATLMENTL
                     KALELRELA"
     gene            2106..2528
                     /gene="dksA"
                     /locus_tag="VNZ84_07090"
     CDS             2106..2528
                     /gene="dksA"
                     /locus_tag="VNZ84_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HWU66759.1"
                     /translation="MADTVNKSFTPYQPAAGEEYMNKKQQDHFRKILNDWKAELSHDI
                     DRTVHTMQDEVTSFADPNDRASQESDMALELRNRDRERKLIKKIDETLRNIDSGDYGY
                     CEGCGIEIGLKRLEARPTATLCIDCKTLDEMREKQVAK"
     gene            complement(2609..3733)
                     /locus_tag="VNZ84_07095"
     CDS             complement(2609..3733)
                     /locus_tag="VNZ84_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWU66760.1"
                     /translation="MTLASFAAHPEKTRGRKVIEQESPTRNVFQRDRDRIIHSTAFRR
                     LEYKTQVFVNHEGDLFRTRLTHSIEVAQLTRGIARTLGLHEDLAEAIALAHDLGHTPF
                     GHAGQDALNQCMRDYGGFEHNLQSLRVVDVLENRYASFDGLNLTFEVREGILKHCSRK
                     NAEQLGDVGERFLRKESPSLEAQITNFADEIAYNNHDVDDGLRSGLITIQQLSGIQLF
                     AENMAEVKRLYGDIEEKRLVHETIRRMINVLVVDLCEQSQQAIANLNPATIDDVRKSP
                     VYLVGFSQAIAEKNLELKQFLLKHLYRHYKVNRMSAKAGRIIEELFTAFIQSPNLMPQ
                     EHQQAAKQDQARAVADYIAGMTDRYAIREHRRLFAVEESW"
     gene            complement(3750..4829)
                     /gene="aroB"
                     /locus_tag="VNZ84_07100"
     CDS             complement(3750..4829)
                     /gene="aroB"
                     /locus_tag="VNZ84_07100"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWU66761.1"
                     /translation="MQTLTVSLADRSYPIHIGNGLLGQADLILPYLKRKQVAIVSNTT
                     VAPLYMQTIAQPLRDAGVSVIEIILPDGEAYKNNETLQTIYDHLLQNRCERNTTLIAL
                     GGGVIGDLTGYAAATYLRGVPFIQVPTTLLSQVDSSVGGKTGINHPLGKNMIGAFYQP
                     KLVLADIDTLKTLPQRELSAGVAEVIKYGLIRDADFFSWLETNMSALMALDTAVASYA
                     IYRSCQNKAEVVAADEHEQGERALLNLGHTFGHAIENAMGYGVWLHGEAVATGTVMAA
                     DLSQRMGWLNEAQIARIKTIMQAAKLPIEAPDLGVEEYLRLMQLDKKVSDGRIRLILQ
                     QDIGKAIITADYDADKLKQTLSLAA"
     gene            complement(4817..5359)
                     /locus_tag="VNZ84_07105"
     CDS             complement(4817..5359)
                     /locus_tag="VNZ84_07105"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWU66762.1"
                     /translation="MGAQIPNNIFLIGLMGAGKTTVGKLIAKNLGKTFYDTDHVIEQR
                     TGVKIPTIFELEGEAGFRKRETSTLEEFAQQDNIVLATGGGAIIAPENREILKKYGYV
                     IYLRANVNELYLRTRNDKNRPLLQNVDVKAKLEQLFHARNPLYTETANLIVDTGHQPV
                     AVIIQKIENALKALESSCKP"
     gene            complement(5440..>6932)
                     /gene="pilQ"
                     /locus_tag="VNZ84_07110"
     CDS             complement(5440..>6932)
                     /gene="pilQ"
                     /locus_tag="VNZ84_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HWU66763.1"
                     /translation="RLVVDIRPLVKDPNKLIQGSKQGYAGEKLSLNFQRVDVRDVLKV
                     IADFTGKNIVVSDSVSGSVTIGLKDVPWDQALDVIMKSKGLDMRVNGSVISIAPAEEF
                     AAKEKAQLTADAERETLETLRTEVFSLRYQKAMDFRNMLLGGGTSTAGGSTSSGMSMG
                     TSTGGSSAGRMNRILSPRGSVTFDARTNTIFVQDTPKKLEEIQVIINKVDVPVKQVMI
                     ESRMVIASNTFSKALGARFGISQTGTPGSNTNLSIGGTLGNKATAYTAPTTAGGTGTY
                     TLGTQGGTVQSATIGNYIISSNGQPDLMSNLPVTNAYGGIALSLLKLSANLLLNLELS
                     ALEADSRGKVISSPRVTTANQQKARIAQGVEIPYQSATSSGATAVSFKKAELSLEVTP
                     QITPDQKIIMDLDVRKDSRGETLPGGVAINTQNVQTQVLVGNGETVVLGGIYEQVSQK
                     TTDKVPFFGDLPVVGYAFKRNTRQEDKTELLIFITPKVMDETLALN"
BASE COUNT         1775 a         1781 c         1607 g         1769 t
ORIGIN      
        1 agttatcatt gatccgtttg aaaaaatcaa tatcaaaaat caccagataa tgatgcaggg
       61 ggaaacggta ttcaggtttg taatcattga gcaggatttt gctcacacat tggtcaaaag
      121 ttttacggtt aagcaaccct gtcaggccat cgcgctcatt ttcaataatc aggcgagcgt
      181 agtaatggaa catctgtaag cattgctcta ccaacgcaac ggcctccgta gacaatgctg
      241 catgttggat cagcaaaatt gcatttttgt gcgcaatact ttgggagagc ggaaaaatat
      301 gtaacttgcc tatggctgtt tccaggcatt gatgttggcg atgctcctga tcgagatagc
      361 ccagcaacat tttatagaca tcggctggta aggcttcaaa agctggatcg gtgctggcca
      421 ccttgggatc catgactgag accaactggc gcctggacag tacttgcatc ttaatgggct
      481 gcgcttgccc ggcaaaagtg ccaagccagt gctgcatgag tgaaagcaaa gcgggatcca
      541 aagcgagact ttccctgacc ttagtcaagt caatcaggct ctgcataaat ttttccaagt
      601 ccaaatgtgt agtcatgtgc gtgataaacg tttttaaaca ctttatcaaa agcaaaatag
      661 atgcctactg cgaaacagag cctattttag ggactaattt tggatttaaa gagaggagat
      721 aagtaaatgg ggtggctaat cgggctcgaa ccgacgacaa ccggaatcac aatccggggc
      781 tctaccaaca gagctatagc caccactggg aaagattttg gcctgcccga caggaatcga
      841 acctgtaacc cccagcttag aaggctggtg ctctatccag ttgagctacg agcagatagt
      901 agttacatgc cgatcaggca atccagagaa aactggtcgg cgtggagaga ttcgaactcc
      961 cgacatcctg ctcccaaagc aggcgcgcta ccagactacg ctacacgccg aaagcagaaa
     1021 tataaccgat tttacgaatt tgtgtcaatg tcgatgtgat aaaattatct tttttacgac
     1081 aaattttttg ttcatgagcg cccaaattat tgatggcaaa cagattgcca atcaactgtt
     1141 agatgacatc aaagtcaaca tagaccaacg tttgcaaaat gggaaacgtg cgccttgcct
     1201 ggccgttatt ttggttggcg atgacgccgc ttctgcaatt tatgtgcgca acaaacgcct
     1261 cgcttgcgaa aaagtgggga ttgtctcggt tgcacgcaat ttgcctagca gtacctcaca
     1321 agaagagttg tttgatttga tcaggcaact caacgctgac gatgccacgg atggcatcct
     1381 ggttcagtct ccattacctc atcatataga tgagaccgaa attctggcct tgattaaccc
     1441 agccaaagat gttgacggtt ttcatcctta taacattggc cgcttggccg ttcgccagcc
     1501 gttattacgc tcgtgcacac cttttggtgt aatcaaaatg ttgcaagcca gtggcatttc
     1561 acttaaaggg atggatgcag tggttgtggg tgtctccaat catgtaggga gacctatggg
     1621 cctggaacta ctactggcag gctgcaccgt cactagttgc catcgtcata cgaaagatct
     1681 ggcagctcat attggccgtg ctgacctcat tgtagcggcg gctggcaaag ccggccttat
     1741 taaaggcgag tggatcaaac caggggctgt tgtcgttgat atcggcatca accgcttacc
     1801 ggatggcaaa cttaccggcg atgtggattt tgcagtggcc agccagcgcg ccagccatat
     1861 tacgcctgtt cctggtggcg ttggccccat gaccgtcgcc acattaatgg aaaacacttt
     1921 aaaagcactg gaattgcgcg aactggcata acacaactta ccaaacgctt gcctataaag
     1981 gtgaaagaca ctttgcaacc ccggaaaaac tggtgtaaac tcgcgcgatt gttgattgac
     2041 gtaatgcaat acacaacgct ctcacgggca ataactattc agtagtttcg ttctttggaa
     2101 ccatcatggc agacaccgtt aacaaatctt tcacccctta ccagccagcc gccggtgagg
     2161 aatatatgaa taaaaaacag caggatcact tccgtaaaat cctgaatgac tggaaagctg
     2221 aactgagtca cgatattgac cgcaccgtgc ataccatgca ggatgaagtc accagttttg
     2281 ccgacccgaa tgatcgtgcc agccaggagt ctgacatggc ccttgagttg cgcaaccgcg
     2341 accgcgagcg caagctgatc aagaagatcg acgaaacact gcgcaatatt gatagcggcg
     2401 actatggata ttgtgagggc tgtggcattg aaattggcct gaaacgcctg gaagcgcgtc
     2461 cgacggcaac cttatgcatt gattgcaaaa cgctggatga aatgcgcgaa aaacaagttg
     2521 ccaaataatc cactcggcga cgcataacaa aaaaggctgc attgatgcag ccttttttat
     2581 ttggcataca gcagctatca cttagctgct accagctctc ttcaaccgca aacaaacgtc
     2641 tgtgctcacg aatcgcatag cgatctgtca tcccagcaat atagtcagcg acggcgcgag
     2701 cctggtcttg ttttgcagct tgctggtgct cttgcggcat cagattgggg gactgtataa
     2761 aggcagtaaa cagttcctca ataatccgcc ctgcttttgc actcatgcga ttcactttgt
     2821 agtgccgata caaatgctta agcaaaaact gcttcaactc gagatttttc tcggcaatcg
     2881 cctgactgaa gcccaccaaa tacacaggtg atttacgcac atcatcaatc gtcgcagggt
     2941 taaggttggc gattgcctgc tggctttgtt cacacaaatc gaccaccagc acattaatca
     3001 tgcggcgtat ggtctcgtgg accaagcgct tttcttcaat gtcaccatac agccgcttca
     3061 cttcagccat attttcagca aataactgga tgccagataa ctgctgaata gtaatcaggc
     3121 cagaacgcag accatcatcg acatcgtggt tattgtaggc aatttcatcg gcaaaattgg
     3181 taatctgcgc ctctaaactt ggcgactctt tacgcaagaa gcgttcgcca acatcaccca
     3241 actgttcggc atttttacgg gaacaatgtt tgagtatgcc ttcacgcact tcgaaggtca
     3301 gattcagtcc atcaaaactg gcgtatcgat tttccagcac atccaccaca cgcaaagact
     3361 gcaggttatg ttcaaagcca ccataatccc gcatgcactg attaagcgca tcctggccag
     3421 catggccaaa aggcgtatgt ccaagatcat gtgctaatgc gattgcttca gccaggtctt
     3481 catgcaaccc caaggttcgc gcaatgccgc gtgtcagctg cgcgacttca atactgtgtg
     3541 ttaagcgcgt ccggaacaaa tcaccttcgt gatttacaaa cacctgcgtc ttgtactcca
     3601 gccggcgaaa cgcagtagag tgaataatgc ggtcacgatc gcgttgaaac acattacgcg
     3661 taggcgactc ttgctcaatc accttgcggc cccgtgtttt ttcgggatgt gcagcaaaac
     3721 tggccaaggt catcacattc actttagtat caagcggcca atgacaaagt ttgttttagt
     3781 ttgtcagcat cgtaatctgc ggtgatgatc gccttgccaa tatcctgctg caaaatcagg
     3841 cggatgcggc catcggacac ttttttgtct aattgcatca atcgcaaata ctcttctacc
     3901 cccaagtctg gtgcctcaat cggcaatttg gctgcttgca taatggtttt aatacgtgcg
     3961 atttgcgcct cattcagcca gcccatgcgc tgcgataaat ctgcagccat caccgtgccg
     4021 gtagccacgg cctcgccatg caaccagacc ccatagccca tggcgttttc gattgcatgg
     4081 ccaaaggtgt gtcccaaatt aagcaaggcg cgctcgcctt gctcatgctc gtcggccgcc
     4141 actacttccg ccttgttctg gcaggagcgg taaatggcat agctggccac cgctgtatcc
     4201 agcgccatca gcgcggacat attggtttcc agccagctaa aaaagtcagc atcacgaatc
     4261 aagccatact taatcacctc tgccacaccg gcggataatt cgcgctgtgg cagggttttc
     4321 aaggtatcga tatccgccag caccagtttt ggctggtaaa acgcgcctat catgttcttg
     4381 cccagcgggt ggttgatacc cgttttgcca cctacgctgg agtccacttg cgacaataac
     4441 gtcgtgggta cctggataaa tggcacgcca cgcagataag tagccgcagc ataaccggtc
     4501 aagtcgccaa tcaccccacc acccagcgca atcagggtcg tattccgttc acagcgattt
     4561 tgcaacagat gatcgtaaat ggtttgcagt gtttcattgt ttttgtaagc ctcgccatct
     4621 ggcagaataa tttcaatgac actgacccct gcgtcacgca agggctgggc aatggtctgc
     4681 atatacaatg gcgctaccgt cgtattgctg acgatggcaa cttgcttgcg cttcaaatac
     4741 ggcagaatca gatctgcctg gcccagtagg ccattaccga tatgaatagg gtaactacgg
     4801 tcagccaggc tgacggtcaa ggtttgcatg atgactccaa cgctttcagg gcgttttcaa
     4861 ttttctggat aatcacggcc accggctggt gaccagtatc gacgatgaga ttggcagttt
     4921 ctgtataaag tggattgcgc gcatggaaca attgttccag cttggctttg acatccacat
     4981 tctgtaacaa gggacgattt ttatcgttac gggtacgcaa atataactcg ttcacatttg
     5041 cccgcaagta aatcacgtaa ccatactttt tgagaatctc ccggttttcg ggtgcaataa
     5101 tggcgccgcc gcctgtggcg agcacaatat tatcctgttg ggcaaactct tccagagtgg
     5161 aagtttcacg tttgcggaac ccggcctcgc cttccagctc gaaaatggtc ggaattttga
     5221 cgccggtgcg ttgttcaatg acatgatcgg tgtcgtaaaa ggtcttgccc agatttttgg
     5281 caatcagctt tccaacggtg gtcttgcctg cccccatcaa tccgatcaaa aatatgttgt
     5341 ttggtatttg tgcacccata aaaaagccca accgttccag ttgggcattt taaggtaaag
     5401 cctggccgct gtcagtaaca gcggcgttga gaataggggc taattaagtg ccagcgtttc
     5461 atccatcact tttggcgtaa taaaaatcag caattcagtt ttatcttcct gcctagtatt
     5521 acgcttgaac gcatacccaa ccactggcaa atcaccaaaa aatggcacct tgtcagtcgt
     5581 tttttgtgag acttgctcat aaatcccgcc cagaaccaca gtttcaccat taccgaccaa
     5641 tacttgtgtt tgcacattct gcgtattgat agccacgcca cctggcagcg tttcaccacg
     5701 gctgtccttg cgtacatcca gatccatgat gattttctgg tccggggtaa tttgaggcgt
     5761 cacctccaaa ctcaattccg cctttttgaa gctgacggct gtcgcaccac tgctggtcgc
     5821 actttggtaa ggaatctcta caccttgcgc aattcttgcc ttttgctggt tggccgtcgt
     5881 gacacgtggg cttgagataa ccttaccgcg ggagtctgcc tccaatgctg acaattccag
     5941 attcaacaat aagttagctg acagcttaag caaactcagg gcaatgccac cataagcatt
     6001 ggttaccggc aaattggaca ttaaatcagg ctgcccgttg gagctgataa tatagttacc
     6061 aatggtcgca ctttgtactg tgccaccttg tgtgcccaaa gtataagtcc ctgtgccacc
     6121 tgccgtcgtt ggtgcagtat acgcggttgc cttattaccc agcgttccgc ctatagagag
     6181 gttggtgttg ctaccgggag tcccggtttg tgagatgcca aaacgcgcac ccagcgcttt
     6241 tgagaaggta ttggaggcaa tcaccatccg tgactcgatc atcacttgct tcaccggcac
     6301 atcaaccttg ttaatgatca cttgaatttc ttccagtttt tttggtgtat cctgcacaaa
     6361 aatcgtattg gttcgtgcat caaaggtcac actcccacgc ggcgaaagaa tacggttcat
     6421 acgtccagcc gaagagcctc cggtagatgt ccccatggac atgccggaag aagttgagcc
     6481 gccagccgtg ctggtaccac cacccagcaa catgtttctg aaatccatgg ctttttggta
     6541 tctcaatgaa aagacttccg tacgtagtgt ttctaaggtc tcgcgctccg catctgctgt
     6601 cagctgtgct ttttccttgg cagcaaactc ttcagcaggt gcaattgaaa tcacactgcc
     6661 attcacgcgc atatccaggc ccttgctctt cataatcaca tccagcgcct ggtcccaagg
     6721 cacatcttta aggcctatgg tgacactgcc agaaacagaa tcgctcacca cgatattttt
     6781 acctgtgaag tcagcaatca ctttcaggac atccctgaca tcgacacgtt gaaaattaag
     6841 cgacagcttt tctccggcat agccttgctt ggagccctgg atcagcttat tcgggtcttt
     6901 taccaaaggg cggatatcca ccactaagcg gc
//
LOCUS       DATMSK010000211         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_15844,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000211 DATMSK010000000
VERSION     DATMSK010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15844"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..118
                     /locus_tag="VNZ84_07115"
     tRNA            43..118
                     /locus_tag="VNZ84_07115"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Lys,seq:ttt)
     gene            539..1261
                     /locus_tag="VNZ84_07120"
     CDS             539..1261
                     /locus_tag="VNZ84_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66764.1"
                     /translation="MFDNVDVIATQGNMGKEAGLYINLSHGFAYAHEHYEFKVLLRLD
                     TDALVIGPDPEKDAIECFDQHPELGMLGSYRVDCNGDPRDFSWPRRKLTQKLKVNLAL
                     LRHPGAWRSWWFIYRVSRRSIKYGYEPGEHCLGGAYFVSAECIRRLVRHQLLARKEFF
                     WSELQEDQIFGLLVRAVGMQHGDFSTGSFPMGLRWRGLPDSPATLVANKKKVTHSTRF
                     FGDENEFAIRTFFKAQRSLDQV"
     gene            1351..1887
                     /locus_tag="VNZ84_07125"
     CDS             1351..1887
                     /locus_tag="VNZ84_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HWU66765.1"
                     /translation="MALKSTVFKASLQIADMERHYYHDHALTLARHPSETDERMMVRL
                     LAFALHADEQMAFGQGMTDDEEADLWRRDLTGRIEQWIDVGLPDEKLIRKACGRADEV
                     VVYAYGGRTAEMWFAQNASGFARLSNLRVYSLPQETTQALAALAQRSMNLQCTIQDGQ
                     VWLSDGEASVEVTRATLK"
     gene            2014..2322
                     /locus_tag="VNZ84_07130"
     CDS             2014..2322
                     /locus_tag="VNZ84_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWU66766.1"
                     /translation="MKTNFKALLLVSALAMPLSGFAADGDAVSTKLSDTVITTKVKAA
                     FAKDKLVSASDIKVETDSNGLVELSGTAKTKKEAEKAVLLAKGVKGVTAVKNEIVVAE
                     "
     gene            2533..3165
                     /locus_tag="VNZ84_07135"
     CDS             2533..3165
                     /locus_tag="VNZ84_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWU66767.1"
                     /translation="MAWGSMRSLGLMLLVTVLSLLAIFFVDAPAALWIKAHLLSYAPF
                     HDYTNNLPSHLFWVVLIGSTFSFSFYFYLRRLGERSPRRDFFLLIGVVLPMAFLAKHV
                     LKLMFARIEVRFWLSNPEAHQAHWFQIGKRGYDAFPSGHMVVFMTVFMALWQFYPRWR
                     RWYLAGMSLLAVALVATNYHYVSDVIAGTYIGMLVYWATRLAVLKERKRG"
     gene            3223..4002
                     /locus_tag="VNZ84_07140"
     CDS             3223..4002
                     /locus_tag="VNZ84_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HWU66768.1"
                     /translation="MEPLNAIKGVLFDLDGVLYIGGKPVEGAMDAVDRIRNAGLPCRF
                     VTNTSTLSLASLQAKLGSLGFAVETEEIISATQAARLYLMRQGDPVCRFLLAEDVMQD
                     FADFRQSDTDAEFIVIGDIGDAWSYRLLNEVFNCLMRGAKLIAIHKNRYWQTEHGLQM
                     DIGAFITGLEYASGAQTTLMGKPSADFFAMALISMSVAACDAVLIGDDIDADIGGAQQ
                     AGLHGVLVQTGKYRQAYCDASTIHPDRVIPSIREFPDLLGI"
BASE COUNT          898 a          981 c         1223 g         1095 t
ORIGIN      
        1 gcgctttcca aagcaacgga aaacatcggg acgaaagtct ccgggctgtt agctcagctg
       61 gtagagcagc ggacttttaa tccgtttgtc gtgggttcga tccccgcaca gcccaccaac
      121 accaaaaagg ggttacgaga aatcgtaacc ctttttttat gtattttgaa tgctggcatt
      181 gatattcata aagcccgtaa tgaatagccc gcgatcaagc ccttgaagag cagcataggg
      241 attcaagttt atgctaatgt ttggctggaa gtgtggttta acgcccaaat agggcgatga
      301 tgtcgttttg gtttttattg ctgtttgccg cagataacta ccttaagagg cgggtcatcc
      361 catgcgaaat gctgccagga aaaatgttga gcttgctgtt gtgctccctg tgggccccac
      421 atgccagtta gcgtatgttg tggatacgat agaaagcatc cgatattaca ttcgctcatc
      481 ccatgtgatc attgtgctgg atgatagtgg cgagtccctt tgcgcaggtc ttccagatgt
      541 gtttgataac gttgacgtca ttgctaccca aggcaatatg gggaaagaag caggcctgta
      601 catcaatctc tcgcatgggt ttgcttatgc ccatgagcat tacgaattca aggtgctgct
      661 acggcttgat acggatgctc tggtcattgg tcctgacccg gaaaaagatg ccatcgaatg
      721 ttttgatcag catccagagc ttgggatgct gggttcgtac cgggtggatt gcaatgggga
      781 tccgagagac ttttcatggc cgcgccgcaa gctgacgcag aagcttaaag tcaacctggc
      841 tttgttacgc catccaggag catggagaag ctggtggttt atctaccgtg tgtctcgtcg
      901 cagtatcaaa tacgggtatg agcccggtga gcactgtctg ggtggcgcgt attttgtcag
      961 tgctgaatgt attcgacgcc tggtgcgaca ccagttgttg gcacgcaaag aatttttctg
     1021 gagcgaactg caagaggatc agatttttgg cctgcttgtt cgtgcggtag gaatgcagca
     1081 tggtgacttc tcaacgggga gttttccgat ggggctacgg tggcgaggtt tgcctgactc
     1141 acccgcgacc ctggtagcga ataaaaagaa agtcacacat tcgacccgct tttttggcga
     1201 tgagaatgag tttgccatac gcaccttttt taaagcgcaa cgtagccttg accaagtcta
     1261 gttcaatcct ggctggggtt gccgttaatg gattgagcgt cgccgtttat aaaaagttgg
     1321 ttttttacat tacttatcaa gattgttttt atggccctta aatccaccgt attcaaagcc
     1381 agtctgcaga ttgccgacat ggagcggcat tattatcatg atcatgcatt gaccctggcg
     1441 cggcatccgt cggagacgga tgagcgcatg atggtgcgcc tgctggcgtt tgccctgcat
     1501 gcggatgagc agatggcgtt tggtcagggt atgacggacg atgaagaggc ggacctttgg
     1561 cgccgcgatt tgactggccg cattgagcag tggattgatg tcggcctgcc ggacgagaag
     1621 ctgatacgca aggcatgtgg ccgtgccgat gaggtggtgg tgtatgccta tggcgggcgt
     1681 acggcagaga tgtggtttgc gcagaacgct tccggttttg ccaggctcag caatctgcgg
     1741 gtttacagtc tgccgcagga aacaacccag gctctggccg cattggcgca acgcagcatg
     1801 aatctgcaat gcaccatcca ggatggtcag gtgtggttaa gcgatgggga ggcgagcgtg
     1861 gaagtcaccc gcgctaccct aaagtagggc gctacgccta cactccagta agcgttctct
     1921 gacacgagaa tcagcgacca acgtattcgc catcatgccg gtttctttca caatggattc
     1981 gtcttcaaca acaacccatt ataaggaaac gatatgaaaa cgaattttaa agctctgctg
     2041 ttggtaagtg ctctggcaat gcctttgagt ggctttgctg cagacggtga tgccgtttcc
     2101 accaagttga gcgataccgt gatcacaacc aaggtgaagg ctgcgtttgc caaggataag
     2161 ctggtaagtg ccagtgatat caaggttgaa actgacagca atggcctggt cgaattgtcc
     2221 ggcactgcaa aaaccaagaa agaagctgaa aaagcagtgt tgctcgccaa gggcgtcaag
     2281 ggcgtgaccg ccgtcaagaa tgaaattgtt gtcgctgagt aatttctcgc gataacgggt
     2341 tgccggtttt gccggtcacc ggaaaaaggc tgacagcaat gtcggccttt tttcatggcc
     2401 gtctgcgtgc gagataaaag caaatctggt cccgtcggcg ctggatcatc gacgcgtggg
     2461 tatcgctagt ctaaacttca ggcatggtca ggatattttt tgagtgcttg gtggcgaatg
     2521 aggtcaggat atatggcgtg gggatctatg cgtagcctgg gtctcatgct gctggtaacc
     2581 gtattgagcc tgctggcgat tttttttgtg gatgcgccag cggccttgtg gatcaaggcg
     2641 catttgctga gctatgcccc gtttcatgac tataccaata accttcccag ccatttgttc
     2701 tgggtggtgc tgatcggctc tacctttagt ttttccttct atttttactt gcgcaggctg
     2761 ggcgagcgta gcccaaggcg tgactttttt cttttgatcg gtgtggtgct gcccatggcg
     2821 ttcctcgcca agcatgtgct caagctcatg ttcgcgcgta tcgaggttcg cttttggttg
     2881 tccaatcccg aggctcatca ggcgcactgg tttcagatag gcaagcgtgg gtacgacgct
     2941 tttccgtcag gtcacatggt ggtgttcatg acggtgttca tggcgctgtg gcagttttat
     3001 ccacgctggc gccggtggta tctggcgggc atgtcactcc tggcggtggc gctggtggct
     3061 accaactatc attatgtcag cgatgtgatc gccggtactt atatcggcat gctggtttat
     3121 tgggctaccc gcttggccgt gttgaaggaa cgtaaacgtg gatagggttg gcatcgttga
     3181 tgcttgcatg caagtgttat ccgtatgcag aaaggagtca tcatggagcc gctaaatgcc
     3241 atcaagggcg tgctgtttga tctcgacggt gtgctctata tcggcggcaa gccggtggag
     3301 ggcgcgatgg atgcggtaga ccgtatccgc aacgccggtt tgccgtgccg gtttgttacc
     3361 aataccagca cgctgtcgct ggcttcattg caggcaaaac tgggcagtct gggctttgcg
     3421 gtggaaacag aggaaatcat cagtgccacc caggccgcgc gtttatatct gatgcggcag
     3481 ggcgacccgg tctgccggtt cctgctggcg gaagatgtga tgcaggattt tgccgatttt
     3541 cgccagtcag ataccgatgc cgaattcatt gtgattggcg atattggcga tgcctggtca
     3601 taccggttgc tgaatgaggt gttcaactgc ctgatgcgtg gcgccaagct gattgccatc
     3661 cacaagaacc gctactggca aaccgagcat ggcttgcaaa tggatattgg cgcctttatc
     3721 accggtctgg aatatgcaag tggcgcgcag accacgctga tgggcaagcc ctcggcggat
     3781 ttttttgcca tggcgctgat cagtatgagt gtggcggcat gcgatgcggt gctaatcggc
     3841 gatgacattg atgccgacat tggcggtgcc cagcaggcag gtctgcatgg ggtgctggtg
     3901 cagaccggca agtatcgcca ggcatactgt gatgcctcca ctattcaccc ggatcgggtg
     3961 attccatcca tacgcgaatt tcccgatttg ctggggattt agtggcgcga ttgtcgccat
     4021 ttgccgaccc cgcatgccgg ggtcagcgaa ttttttcggc cttatgtagt catcttgaat
     4081 aagaagcccg gcaagcgtgc cgttctgcta actcactaat caatgtcagt ttcaaggctc
     4141 cccgtggggt gcgagcgaac tttaataaag tcactgcctg caggcatgtc gacatca
//
LOCUS       DATMSK010000212        11527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_86422,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000212 DATMSK010000000
VERSION     DATMSK010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86422"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VNZ84_07145"
     CDS             complement(<1..372)
                     /locus_tag="VNZ84_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66769.1"
                     /translation="MSAFARFLKWVFWIILLPLGLLSLYTWGSLTWVYSDGERVGYVQ
                     KLSHKGWLCKTWEGELVLVTVPGTQAEKFYFTVRDPAVIPKINALSGERVRLQYEEHK
                     GVPSSCFGETSHFVNAVDEIKE"
     gene            500..3268
                     /locus_tag="VNZ84_07150"
     CDS             500..3268
                     /locus_tag="VNZ84_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWU66770.1"
                     /translation="MTATDHFPALSLAHSDTVDSGTVILCASARLAQGIRLWLQQSYQ
                     QQGLSQWQAPHVFPLQDWLNQRIEYAILSGQIDAAQAPLGLLSATQEALLWEQAIQHC
                     LRQHEALDLLNTNGLASAAMEANRYLIEWNISLDMAAASEETRQFMQWRQQFQALCKQ
                     SGYLEGVRYQAWQLTQLKQNSLPLPASIQLAGFDRLNPHIQALINILQASGVEVSQFS
                     NGIAAQSTQRMAFAEAMDEMRATVHWAQQQLAAQPQAKLAIVVPELDTQRDTLANLLD
                     DTFHPETLHPAHAETPRCYEFSLGLPLHRWPLVHTALSLLRLAFQAAPLAQSELSALL
                     ADIYWSQPLHEADARAALDAEMRRQLPLQVHTHTFARFVARKQQDEFPIHCPALQEDL
                     SALLNGAKQTPRRQLPSAWSTSFSQLLTATHWPGERGLSSHEHQCQTKFQAVLSQLSA
                     LDAWLGPVDAMSAIHRLSQLCQAQIFQPQTVRQPNITVMGMLEASAQPLDGIWVMGMN
                     DHVWPPLSRTNALIPAESQRQAGTPNASSEVQMAFASQIHQRLLQSAKEIIFSYANKD
                     CDRLLRMSPLVQPIPEVSAINGAHTLAETLARNSSRDWQWLDDHQAPPVEDGEHISGG
                     TGLLRAQALCPAWAFYQYRLKARKLETPHNGLDAMQRGDLIHRVLAAFWSQQSTLDWQ
                     TVAVDDLKAQLDQIAVAVIAAFNQEFSQPFSAVFCQLEAERLSKLALTWLWEVERERR
                     QAYTVTSVEQVFKPLIEGIQVKLMIDRVDTLADGRLVVVDYKTGSMPDFKNWASDKIS
                     EPQLPIYAAFLLQDAEIAAVCFGKLRLTDGGFAGVAAEDDIVPGIKAFNHEKNKLFDP
                     DQFPDWPSVLTHWRTQITRTAQALKAGEAAVVFDAEQELGYCDVLPLLRLPERQLQFE
                     HQQAGGAS"
     gene            3265..6639
                     /locus_tag="VNZ84_07155"
     CDS             3265..6639
                     /locus_tag="VNZ84_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWU66771.1"
                     /translation="MTMTDTAFQQALVLDATNRERALAVESFIVEAPAGAGKTELLTQ
                     RFLKLLQTVQAPEEIIAITFTNKAASEMRARILDSLLLAAAGVPPEAPHKQITYTLGQ
                     QALKRSGELQWHLLESPARLRIYTIDSLCANLARQMPLLSRFGTQPAVTEDAWAYYRE
                     AAGLALQAMDEDTLGEPVRRVLRYMDNDQARLEALLMSMLAKREQWLHLSQTNDREQA
                     QDALIYLIESELQAALTALPARLQQSLMPIARYAASQLPPEHILAQLLDWQIPFTATI
                     EDLPGWRALAELLLTSTGGLRKQINKNIGMPATDEAKPYKEVLTDILADLAQHSDSER
                     SLARIRLLPDTSNENDDIIQALSQLLNIAAAQLWLCFQAQNEVDFVEIAQRALKALQE
                     GDEATELAMRLDYRIQHLLVDEFQDTSPMQIDLLKALTRGWQPGDGRTLFAVGDPMQS
                     IYRFRKANVGLFLNAARYGIGDIALTPLKLWRNNRSCPPVVDWINQTFAGMLPAQDAP
                     QQGAIAYRPFVATRADAANAGVFVHPLIAPAQSEEEEPADIRHVEAEQIIRIIQQTRA
                     EKPDASIAVLVRARKHLHALVTEMRRNHSELSFQAVEIEELANRQIVQDLLTLTYALH
                     QRADRVHWLALLRAPWCGLTLADLHALVGQDGQRSVLSLLADETIVTRLSVDGQARAR
                     HVYDVMQSALHWRGRTTVSRWVHNTWLRLDGAACLWNENDVQDVQAFFSRIAALEQHG
                     QFNPQALAEDVQKLYAAPDAKADTRLQFMTIHKSKGLEFDTVILPGLDGSNPPDDAKL
                     VIWEEVPMPDGHTELVAAPFVPTVLKRQQEIITAYDYLNDLDKTRAAYEDARVLYVAA
                     TRAERQLHLLGVAKPDKSGTPQARKNSFLHLLWPAIQHWFTSEHDIFTRYPVTQQQAD
                     IRQFVPKLIRLREAQSPAMLQATLAETPSGYPSTTDADEVNRLEADIGTLAHLYLQLI
                     AEQGMVAWETHAQQAFSSLLIPMQRWFKQQGYADREADQGANQVAALLTTTLQSDDGR
                     WVLQPHTQGASELAIEQLSERKKVIDRTFVADGVRWIVDYKSMPVTAKDDLPLLASAF
                     KSQLAAYAQLFQAEGLPIKTAILFLSAGKLAPVDIH"
     gene            6649..7326
                     /locus_tag="VNZ84_07160"
     CDS             6649..7326
                     /locus_tag="VNZ84_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWU66772.1"
                     /translation="MPEKNTLIEKQAILDAFNFRHACKEFDASKQIPADEFELILEAA
                     RLSPSSFGFEPWHFIVVQDKALRESLKQHAWGAPLKLDTASHFVLCLAMKSPFLDPKG
                     PYLPNFMREVQHHPQEVADMRLGFYTQFQQSDFDLTDERKLFDWASKQCYLPLANMMT
                     VAAMRGIDSCPIEGFKQKETDALLAEQFGVNLQEYGLAYMVAFGYRKVDPRPKTRRAM
                     DQVVSWK"
     gene            complement(7369..7989)
                     /locus_tag="VNZ84_07165"
     CDS             complement(7369..7989)
                     /locus_tag="VNZ84_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWU66773.1"
                     /translation="MLEKLLAAVASWIIAVIATMGYGGVVLLMAIESACIPLPSEIIM
                     PFAGFLVSKGDLTLWGIALAGAVGCVVGSIPAYYLGMYGGRPLVEKYGKYVLISKKDL
                     DMADRWFANHGEIIIFIARLLPAVRTFIAFPAGVARMNMTRFVAYTFVGSLIWCWVLG
                     YIGMKTGEHWEDLKVYFHESHYLIAALGVVFLAWYVRRHFKNEQES"
     gene            complement(7989..11381)
                     /gene="mfd"
                     /locus_tag="VNZ84_07170"
     CDS             complement(7989..11381)
                     /gene="mfd"
                     /locus_tag="VNZ84_07170"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWU66774.1"
                     /translation="MAVSLPVIGHPQRFSFQSQGLDSQALAELACGLKATQTPLVVLT
                     ASAFEAQRLMDEIPYFGKDIQVHLLPDWETLPYDVFSPHPDLTSERLATLYQLSQNQS
                     NVVIVPMATALLRLPPVSYLAAHTFMLKKGQKLDVDALRNQCAQAGYHHVSQVMGPGE
                     FSVRGGLVDLYPMGSAFPYRIDLFDDEIETIRTFDIDTQRSVYPVPEIRMLPAREFPM
                     DEAAITRFRSQFREYFEGDPQRAKIYKDVSKGTPSGGVEWYLPLFFEQTATLFDYLPA
                     QTVLCLHGDLDKAAQTFWADANSRYKLMAYDAEKPILRPESLLQKADEFFKQTHAFAL
                     VYQQLELQKLLPAVDVDRRTEHPLHKLQGFISQFDGRVLIAAESLGRRETIAQLFTEH
                     GVTFSLAEDWAGFAQSNFPVMLGVAPLHQGLVDGDKAVITEAELYASTVRQQRRRNQE
                     KARNAEGMLKDLSELRLDDPVVHEQHGVGRYKGLVNIDFGEGETELLLIEYFGDDKLY
                     VPVSQLFLISRYSGGPPESAPLHRLGSGQWDKAKKKALKQIRDTAAELLNLYAQRAAR
                     RGHAFTLSLHDYEAFCEGFPFEETPDQLEAIENVIKDMQSGRPMDRLVCGDVGFGKTE
                     VALRAAFVAVMGGRQVAVLVPTTLLAEQHYQNFVDRFAEWPIKIAEISRFRTAKEQAE
                     ALKGLADGSIDIVIGTHRLIQKDVQFKNLGLAILDEEHRFGVRQKEQMKALRAEVDVL
                     TLTATPIPRTLSMAMEGLREFSVISTPPQKRLAIKTFHTEYSEGIIREAVMREFKRGG
                     QVYFLHNEVDTIYVMKEKLEKFLPEARIAIAHGQLRERELEHVMRDFYQQRANLLLCT
                     TIVETGIDVPSANTMIINKADMFGLAQLHQLRGRVGRSHHQAYTYLLTDPHRNITPQA
                     QKRLDAIQLLEDLGAGFHLAMHDLEIRGAGELLGDSQSGEMQEIGFNLYSDMLNHAVK
                     QLKAGKEPDLDAPLGVTTEINLHTPALLPNNYCPDVHERLVIYKRLANCEDDDALDAM
                     QEELIDRFGLLPEPGEALIACHRLRIAAKPLGIIKIDASDAAIQLQFNPKADIDPMKL
                     INLLQRDKRCRMNGPDKLRVTVQLGNLNHRVELVKTLLKEFM"
BASE COUNT         2880 a         3199 c         2881 g         2567 t
ORIGIN      
        1 ttctttaatt tcgtccaccg cattcacaaa gtgactggtc tcaccaaaac agcttgaggg
       61 cacaccttta tgttcctcat attgcaggcg tacccgttct ccggataatg cattgatttt
      121 gggaatgaca gcggggtcgc gcaccgtgaa ataaaatttt tctgcctgcg tgcctggcac
      181 cgtcaccagt acaagctccc cttcccaggt tttacatagc cagcccttat gcgatagttt
      241 ctgcacatac cccacacgct cgccatcaga ataaacccag gtcaggctgc cccaggtata
      301 aaggctcagc aaacctaacg ggagcaagat gatccagaat acccatttta aaaatctggc
      361 aaaagccgac atgtcactac tccgcaagtt ttttatcgtt tgttcgtcgc attatcttat
      421 aaaatgttgc gataaagata ggtcgcttct ataaattcaa attgctaagc cagacaacca
      481 cttctactca cggttaccca tgactgccac tgatcatttt ccagcgttaa gccttgcaca
      541 ctctgacact gtggactcag gcaccgtgat tctgtgtgca tcggcacgtc tggcacaagg
      601 catacgctta tggctgcaac agtcctacca gcagcaaggt ctgtcacagt ggcaggcacc
      661 gcatgtattt ccgttacaag actggctcaa ccaacgcata gaatatgcga tcttatctgg
      721 gcaaattgat gccgcccagg caccactggg tttactctcc gccacgcaag aagccctgct
      781 ctgggaacag gctatccagc attgcctgcg acaacatgaa gcactggatt tactcaatac
      841 gaatggcctg gccagcgctg cgatggaggc taaccgttac ctgattgaat ggaatatcag
      901 cctggacatg gcagcggcga gtgaagaaac acgccagttt atgcaatggc gccagcagtt
      961 ccaggcatta tgcaagcaaa gcggctatct ggaaggcgtg cgctatcaag cctggcaact
     1021 cacgcaactc aaacaaaact ccctgccttt gcccgccagc atacaactgg cgggatttga
     1081 tcgcctcaac ccgcatatac aggcactgat caacatcttg caagcctctg gcgtagaagt
     1141 cagccaattc agcaatggga ttgcagcgca aagtacgcaa cgcatggcat ttgccgaagc
     1201 catggacgag atgcgcgcga ccgtccactg ggcacaacag caactggcgg cgcagccgca
     1261 agccaaactc gctattgttg tgccagagct cgatacgcag cgcgatacac tggcaaacct
     1321 gctggatgat actttccatc ctgaaacatt gcatcctgcc cacgcggaga caccgcgctg
     1381 ctacgagttt tcactgggat taccgcttca ccgctggccg ctggtacaca cggcattaag
     1441 cttgttaagg ttagcttttc aagctgcgcc gttagcacaa tccgagcttt ctgcactgct
     1501 ggcagatatc tactggtcac aacccctaca tgaagccgac gcccgggctg cgctcgacgc
     1561 cgaaatgcgc cgccagctgc cactacaagt gcatacccat acgtttgccc ggtttgtcgc
     1621 gcgcaagcag caagatgaat tcccgattca ctgtcctgcc ctgcaagaag atttaagtgc
     1681 tttgctcaat ggtgcaaaac aaacaccccg caggcaatta ccttctgcct ggtctacaag
     1741 ctttagtcaa ttactcaccg caacacattg gccaggcgaa cgtggtttat ccagccatga
     1801 gcaccagtgc cagactaaat tccaggcggt actcagccaa ctctccgcac tggatgcctg
     1861 gctggggcca gtggatgcca tgagcgcaat acacagatta tcgcaactat gtcaggcaca
     1921 gattttccag ccgcaaactg tgcgccagcc gaatatcacc gtcatgggca tgctggaagc
     1981 cagcgcacaa ccgctggacg gcatctgggt catgggcatg aatgaccatg tatggccgcc
     2041 gctgtcacgg acaaacgcgt taataccggc cgaatcacag cgccaagccg gcacacccaa
     2101 tgccagcagt gaggtgcaaa tggcctttgc cagccagatt caccaacgtc tgctgcagtc
     2161 agctaaagag attatttttt cttacgcaaa taaagattgc gaccgcctgc tgcgcatgag
     2221 cccgttggta caacccattc ctgaagtgag tgccatcaat ggcgcgcata cccttgctga
     2281 aacattggcg cggaatagct cgcgagactg gcaatggctg gatgaccacc aggcaccacc
     2341 ggtagaagac ggcgaacata tcagcggcgg caccggtttg ttacgcgcgc aagcactctg
     2401 ccctgcctgg gcgttttacc aataccgcct caaggcacgc aagctggaaa cgccgcacaa
     2461 tggactggat gccatgcagc gtggtgattt gatccaccgc gtgctcgcag ccttttggtc
     2521 acaacaaagc acgttagact ggcaaaccgt ggctgtcgac gacctaaagg cacaactcga
     2581 ccagatcgcc gtagccgtga ttgctgcctt taaccaggag ttttctcaac cgttctcagc
     2641 cgtgttttgc caacttgagg ctgagcggct gagcaagctg gcgctgacct ggttatggga
     2701 agtcgaacgt gaacgccggc aagcttatac tgtgacctcg gtcgaacaag tgttcaaacc
     2761 gctcattgaa ggcatccagg tcaaactgat gattgaccgc gtcgacaccc tggccgatgg
     2821 ccgcctggtc gtggtggatt acaaaaccgg cagcatgcct gattttaaaa actgggcgtc
     2881 ggacaaaatc agcgagccgc aattaccaat ttatgccgcc ttcctgttac aggacgccga
     2941 gattgccgca gtctgttttg gcaaactgag gctcaccgat ggcggatttg ccggggtcgc
     3001 cgccgaagac gatattgtac ccggcattaa agcgttcaat cacgagaaaa ataaactgtt
     3061 tgaccctgac cagttccccg attggccttc ggtattgacg cattggcgca cgcaaatcac
     3121 acgcactgct caagcgctca aggcaggaga agctgccgtc gtgtttgatg ctgaacaaga
     3181 actgggttac tgcgatgtgc tgcctttatt acgcctgcct gaacggcaat tacagtttga
     3241 gcaccagcag gcaggaggcg cgtcatgaca atgactgata cagcgtttca gcaagcactg
     3301 gtactggatg ccaccaatcg cgagcgcgca ctggccgtgg agtcttttat tgttgaagcc
     3361 ccggcgggtg caggtaaaac cgagctactg acacagcgat tcctaaaact gctgcaaacc
     3421 gtgcaggcgc ctgaagaaat catagccatt acgtttacca ataaagccgc ctcggaaatg
     3481 cgcgcacgta ttctagatag cctgctgtta gccgcggcag gtgtgccgcc tgaagcgcca
     3541 cataaacaaa tcacgtatac attaggtcag caggccttaa agcgctcagg cgaattgcag
     3601 tggcacttgc tggaaagccc ggcacgacta cgtatttata ccatcgactc gctctgtgcc
     3661 aacctggcta gacaaatgcc attattgagc cgttttggta ctcaaccggc cgtgactgaa
     3721 gatgcctggg cctattaccg cgaagcggcg ggactggcgc tacaagccat ggatgaagat
     3781 acgctaggcg aacccgtgcg tcgcgtactg cgctatatgg ataacgacca ggccaggctg
     3841 gaagccctgc tcatgtccat gctggccaaa cgtgaacaat ggctgcactt aagccagacc
     3901 aacgaccgtg aacaggcgca agacgcactg atttacttaa tagaaagtga gttacaggca
     3961 gcattgactg cactccctgc ccgtttgcaa caatcgctga tgccgattgc gcgctatgct
     4021 gccagccagt taccgcctga acatatcctc gcgcaattgc tggactggca aataccgttc
     4081 acagcaacca tagaagattt gcctggctgg cgcgcactgg ccgaattact gctgactagt
     4141 actggcggtt tacgtaagca gattaataaa aacataggca tgcccgccac cgacgaagcc
     4201 aagccataca aagaggtgct gaccgacatt ctggcagacc tcgcccaaca cagtgacagc
     4261 gagcgtagtc tggcacgcat ccgcctgttg ccggatacca gcaacgaaaa cgatgacatc
     4321 atccaggccc tcagtcagtt actgaatatt gctgcggcgc agctatggct gtgctttcag
     4381 gcgcagaacg aagtggattt tgtcgaaatt gcccagcgcg cactgaaggc gctgcaagaa
     4441 ggtgatgaag ccaccgagct ggccatgcga ctggactacc ggattcagca tttactggtc
     4501 gatgagtttc aggacaccag cccgatgcag attgacctgc tcaaggcctt gactcgcggc
     4561 tggcagcctg gcgatggccg taccttgttt gccgtcggcg accccatgca atcgatctac
     4621 cgtttccgca aagccaatgt gggcctgttt ctcaacgcgg cacgctatgg cataggtgat
     4681 attgcgctca cgccgctcaa actctggcgc aacaaccgct cctgtccacc cgtcgtcgac
     4741 tggattaacc agacctttgc cggcatgttg cctgcgcaag atgcaccaca gcaaggcgcg
     4801 attgcatatc gtccatttgt cgctacgcgt gcggatgccg ccaacgcggg ggtgtttgtg
     4861 catcccctga ttgcgcctgc gcagagcgaa gaggaagagc cagctgatat tcgccacgtg
     4921 gaagcggagc agattatccg cattatccag cagacacgcg ctgaaaaacc tgacgccagt
     4981 attgccgtac tggtacgtgc gcgcaaacac ttgcatgcac tcgtgaccga aatgcgccgt
     5041 aaccatagtg aactgagttt tcaggcggta gaaattgaag aactcgccaa ccggcagatt
     5101 gtgcaggacc tgttaaccct tacctatgct ctgcatcaac gtgctgatcg tgtacattgg
     5161 cttgcactgc ttagggcgcc gtggtgcggg ctgacgctgg ccgacctgca tgcgctagtc
     5221 ggccaggatg gtcagcgcag tgtactcagc ctactcgctg acgaaaccat tgttacccgt
     5281 ttaagtgtcg acggccaggc acgtgcgcgc catgtgtacg acgtcatgca aagtgcattg
     5341 cactggcgtg ggcggaccac ggtgagccgc tgggtacata acacctggct acgcctggac
     5401 ggcgcagcct gcctgtggaa tgaaaatgat gtgcaggatg tgcaggcttt ttttagccgc
     5461 atcgccgcgc tggagcagca tggacaattt aacccgcaag cactggctga agacgtgcaa
     5521 aaactctatg ccgcgcccga tgccaaagcg gacacccgcc tgcaattcat gaccatccac
     5581 aaatccaaag ggctggagtt tgataccgtg attctgcctg gtcttgatgg cagcaaccca
     5641 cccgatgacg ccaaattggt gatttgggaa gaagtaccca tgcccgacgg gcataccgag
     5701 ctggtggctg cgccttttgt gcccacggtg ttaaaacgcc agcaagaaat catcaccgct
     5761 tacgactatt taaatgacct agacaaaacc cgcgcggctt atgaggatgc tcgcgtgctc
     5821 tatgtagcgg caacccgtgc tgaacgccag ctgcatttgc tgggggtcgc caaaccggac
     5881 aaaagtggca caccacaagc acgcaaaaac agttttctac acttactctg gcctgccatc
     5941 cagcattggt ttaccagtga gcatgatatt ttcacccgct atccggtcac acaacagcag
     6001 gcggatatcc ggcagtttgt gcccaaactg atccgtttac gtgaggctca atcgccagcc
     6061 atgttacaag caacactagc ggaaacgccc tcaggttatc catcaactac cgatgcagat
     6121 gaagtcaacc gccttgaagc agacataggc acactcgccc atttgtattt gcaactgatt
     6181 gctgaacaag gcatggtagc gtgggagacg catgcgcaac aggctttttc atctctgctt
     6241 attccaatgc agcgctggtt caagcaacaa ggctatgcag accgtgaagc agaccaaggg
     6301 gccaaccagg tcgcagcatt actcacgacc acattgcagt ccgacgatgg acgctgggtg
     6361 ttacagcctc atacccaagg cgcctctgaa ttggcgattg agcaactcag tgagcgtaaa
     6421 aaggtcatag accgtacctt tgttgcggat ggtgtgcgct ggattgtgga ctataagtcg
     6481 atgccggtga cagccaagga tgatctgcct ttgctagcaa gcgcattcaa gtcacaatta
     6541 gcggcctatg cgcaattatt ccaggcggaa ggtctgccga ttaaaacagc cattttgttc
     6601 ttaagtgcag gcaagctagc gcctgtcgat attcattgag gaaacatgat gccagaaaaa
     6661 aacacattga tagaaaaaca agccatttta gacgcgttta acttccgaca tgcctgcaag
     6721 gagtttgatg ccagcaaaca gattccagcc gatgagttcg aactcatcct ggaagcggcc
     6781 cgcctctcgc ccagttcctt tggttttgaa ccctggcatt ttattgtggt gcaagacaag
     6841 gcactgcgtg aaagtcttaa acagcatgcc tggggagcgc cgctcaagct ggataccgcc
     6901 agccattttg tactgtgcct ggccatgaaa tcgccctttt tagaccccaa agggccttac
     6961 ctgccaaact ttatgcgtga agttcaacat cacccgcaag aagtggccga tatgcgcctg
     7021 ggcttttata cccagtttca acaatccgac tttgatctca ccgatgaacg caagctattc
     7081 gactgggcgt ccaagcaatg ttacctgcca ctggcaaaca tgatgaccgt ggccgccatg
     7141 cgcggtatag actcctgccc gattgaaggc ttcaagcaaa aagaaacaga tgctttgtta
     7201 gcggagcagt ttggcgtgaa tctacaagaa tatggcctgg cgtatatggt tgcctttggt
     7261 taccgtaaag tagacccaag accaaaaaca cgccgagcga tggatcaggt agtcagctgg
     7321 aagtaaacct cagcaccaaa ctaaaaagct gccagtaggc agcttttttt atgattcctg
     7381 ttcgtttttg aaatgcctgc gtacatacca cgccaagaaa actaccccca aggctgcaat
     7441 caaataatgt gactcatgga aatacacctt taggtcctcc caatgctcgc cggtcttcat
     7501 gccaatatag ccaagcaccc aacaccagat cagtgatccg acaaaggtat acgctacaaa
     7561 gcgagtcata ttcatgcgcg ccacccctgc cgggaaggcg ataaatgtac gcactgcagg
     7621 caacagacgg gcaataaaga tgatgatttc accgtgattg gcaaaccagc gatccgccat
     7681 atcaaggtct tttttgctga tcagcacata tttgccgtat ttctccacca gaggcctgcc
     7741 gccatacatg cccaggtagt aagccgggat agaccccacc acgcaaccaa ctgccccagc
     7801 cagcgcaata ccccataaag ttaaatcccc tttgctgacg agaaatccag cgaacggcat
     7861 aatgatttca gagggcaacg gaatacaggc agactcaatc gccatcagca acaccacgcc
     7921 gccatacccc atggtcgcaa tgaccgcaat aatccaactt gccacagcgg ctaataattt
     7981 ttctagcatt acataaattc cttcaaaagg gttttcacta attctacacg gtgattgagg
     8041 ttacctaact gcacagtcac acgtaattta tccggcccgt tcatgcggca gcgtttgtcg
     8101 cgttgcaaca gattgatcag cttcattggg tcaatatccg ctttgggatt gaactgtaac
     8161 tgtattgcgg catcggacgc atcaattttg atgatgccta atggttttgc cgcgatacgc
     8221 aggcgatggc aggcaatcag cgcttcacct ggctcgggta gcaggccaaa acggtctatg
     8281 agctcttctt gcatcgcgtc caaggcatca tcatcttcgc agttagccag gcgtttatag
     8341 atgaccaatc gttcgtgcac atccgggcaa tagttattcg gcaacaaggc tggcgtatgc
     8401 aggttgattt ccgtcgtcac gcctaggggc gcatccaggt ctggctcttt accggctttg
     8461 agctgtttca ccgcgtgatt aagcatatcg gaatacaggt taaagccaat ctcttgcatc
     8521 tcaccgcttt ggctgtcacc cagtagctcg cctgcgccgc ggatttcgag gtcgtgcatg
     8581 gcgagatgga agcccgcccc caggtcttcc agcaactgga tcgcgtccag gcgtttttgt
     8641 gcctgtggtg tgatattgcg atgcgggtcg gtcagtaaat aggtgtaggc ttgatgatgg
     8701 ctacggccaa cgcgaccacg tagctggtgc aactgtgcca ggccgaacat gtcggccttg
     8761 ttgataatca tggtgttggc gctggggacg tcaatgccgg tttcgacaat ggtggtacac
     8821 aataacaggt tggcgcgttg ctggtaaaag tcgcgcatga catgctccag ctcacgttca
     8881 cgcaactggc cgtgggcaat cgcgatacgc gcttcgggta aaaacttctc cagcttttcc
     8941 ttcatcacgt aaatagtatc tacctcgtta tgcaagaaat atacttgccc accgcgcttg
     9001 aactcacgca tgaccgcttc gcggataatg ccttcagagt attcagtgtg aaaagtttta
     9061 atcgccaagc gcttttgcgg cggtgtagaa atcaccgaga attcacgcaa gccttccatg
     9121 gccatactca aggtccgtgg aatcggcgtg gcggtcaatg tgagcacatc cacctcggca
     9181 cgcaaggctt tcatttgctc tttctgtctc acaccaaaac ggtgctcctc atccagaatt
     9241 gccaaaccta aattcttgaa ctgaacatcc ttctggatca gccggtgtgt tccaatcaca
     9301 atatcaatac tgccatccgc caggcctttg agggcctcgg cctgttcttt agcagtgcgg
     9361 aatcgtgaga tttcagcaat cttgattggc cattcggcaa aacgatctac aaagttttgg
     9421 taatgctgtt cggcgagcaa agtggtgggt accaataccg cgacctggcg gccacccatg
     9481 acggcgacaa atgcggcgcg caaggcgact tccgtcttgc caaagccaac atcgccgcac
     9541 accagtctat ccatgggccg gccagattgc atgtctttga tgacattttc aatcgcttcg
     9601 agctggtctg gcgtttcttc aaacgggaac ccttcgcaaa aggcttcgta atcatgcagg
     9661 ctcagggtga atgcatggcc gcgacgcgca gcacgctggg cgtacaaatt cagtaattcc
     9721 gccgcagtat cacgaatctg cttgagcgct tttttcttgg ctttatccca ctgaccactg
     9781 cctaaacgat gcaaaggtgc gctctcaggc gggccgccag agtaacgaga gatcaggaaa
     9841 agctgcgaca ccggcacata cagcttgtca tcaccaaaat actcaatcag cagtagctcg
     9901 gtttcgcctt caccaaagtc aatattgacc aggcctttat aacgccctac cccatgctgt
     9961 tcatggacga ccggatcgtc cagccgcagc tccgataaat ctttgagcat gccttcagca
    10021 ttccgtgctt tttcctggtt acggcggcgc tgctgacgca cggtactggc atacagttct
    10081 gcttcggtaa tgacagcctt atcaccatcc accaggcctt gatgcaaggg cgcgaccccc
    10141 agcatgaccg ggaaattaga ctgtgcaaat ccagcccaat cctcggccag ggagaacgta
    10201 acaccgtgtt cggtgaataa ctgtgcaatc gtttcccgac ggcctagact ctccgcggca
    10261 atcaacacac ggccatcaaa ctggctgata aatccttgca acttatgcaa aggatgctct
    10321 gtacggcggt ctacatcaac ggcaggcaac agtttttgca gctcaagctg ctgataaacc
    10381 aaggcaaacg catgtgtctg tttgaaaaat tcgtcggcct tttgtagcaa actttctggc
    10441 cgtaaaatcg gtttttcggc atcataagcc atcagtttgt agcggctatt ggcgtccgcc
    10501 caaaaggtct gcgccgcttt atcaagatcg ccatgcaggc aaagtacggt ttgtgccggc
    10561 agataatcaa acagggtagc cgtctgctca aagaacaaag gcaggtacca ctcgactcct
    10621 ccgctagggg tccccttgct cacatcctta taaatttttg cgcgctgcgg atcgccttca
    10681 aaatactcac ggaactggct gcggaagcgc gtaatggctg cttcatccat cgggaattca
    10741 cgcgcaggca gcatgcggat ttcaggcaca ggatagacgc tgcgttgggt atcaatatca
    10801 aaggtgcgta tagtctcgat ttcgtcgtca aataaatcga tacggtaggg aaacgcactc
    10861 cccatgggat ataaatcgac cagcccgcca cgcacactga actcacccgg ccccatgacc
    10921 tggctcacat ggtggtagcc tgcttgagca cactggttac gcaaagcatc tacatccagc
    10981 ttctggccct ttttgagcat aaaggtatgg gcggccaggt aagaaacagg tggcaacctc
    11041 agcaaggccg tagccatggg cacaatgacc acattgctct gattctggct cagctgatag
    11101 agcgtggcca ggcgctccga ggtcaaatca ggatggggtg aaaacacgtc ataaggcaaa
    11161 gtttcccaat ccggcagcaa atgtacctga atgtctttac caaaatacgg gatttcatcc
    11221 atcagtcttt gcgcttcaaa ggcactggct gtgagcacca ccaaaggcgt ttgtgttgct
    11281 tttaaaccac aggccaactc ggccagtgct tggctatcca ggccctgact ttgaaaagaa
    11341 aaacgctgtg gatgcccgat cacgggaagt gaaactgcca taaaaaatta gaatcttatt
    11401 gctcaggtta gacgagtatt tcttggcgaa tctgccgagt ttgctattat aactgcaagc
    11461 gtcgacagac agtgactgaa tgacacgcaa gacgccagag cgaatagaat acagcacgga
    11521 gaagaat
//
LOCUS       DATMSK010000213         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_463063,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000213 DATMSK010000000
VERSION     DATMSK010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463063"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="VNZ84_07175"
     CDS             <1..1568
                     /locus_tag="VNZ84_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate/oxaloacetate carboxyltransferase"
                     /protein_id="HWU66775.1"
                     /translation="DDVVHAFVKQAADTGVDVFRIFDAMNDIRNLKTAIEAVKSAKKH
                     AIGTLSFTTSPVHDIAYFVNMAKELEALGCDSIGVKDMAGLLTPILAGDLVKALKAAV
                     SVPVHMHSHATSGLASMVMLKSVENGVDIIDTCNSSFSEGASHPTTESLVAALKGTEY
                     DTGLDLGKLQEITAYFKEVRKKYWQFESEFTGVDTRVLVNQVPGGMISNLSNQLKEQG
                     ALNRMDEVLAEIPRVRQDLGYPPLVTPTSQIVGTQAVLNVLTGGRYKSITNEVKNYLL
                     GQYGKAPAAVNADVRKQAVGDAEVITCRPADLLQPEMARLQSEAERFANNEQDVLTYA
                     MFPDIGQTFLQERNAGSLQPEPLLSKEAVTQQTAAARYAPNEFNITLHGETYHIKLTG
                     SGHAGEERKPYYVKVDGISEEVFVETLSEMEVSGGGSNGSKKKAAAVAASGRPRPNHP
                     GCVTTAMPGTIVTVKVNVGDKVNAGDGVLVIEAMKMENEIQASKSGTVVAIHVAKGDS
                     VTPDETLIEIQPE"
     gene            1627..2226
                     /locus_tag="VNZ84_07180"
     CDS             1627..2226
                     /locus_tag="VNZ84_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HWU66776.1"
                     /translation="MPIPSLILASSSRYRREVLEKLHLPFQCVSPEIDETPLADESPE
                     QTSLRLAESKARKVAQTYQNALIIGCDQVATVDGLQIGKPGNHDNAVKQLTMLSGKEV
                     IFHSAICLYNSANQHMQSTIAPYYVKFKSLSPGQIETYLRLEQPYDCAGSAKSEGMGI
                     ALLEYMRGDDPNALIGLPVIVLVDMLMQAGVDVLSASRT"
     gene            2320..>3541
                     /locus_tag="VNZ84_07185"
     CDS             2320..>3541
                     /locus_tag="VNZ84_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HWU66777.1"
                     /translation="MRLSSLLLFSLGSVSLCSTSIAQAEITTVSPQSDQTSVAVTIYN
                     EDLALVKDSRKLNLSSGLSALSFRDVSAQIKPETALLRSLLSNSSLQVLEQNFDFDLL
                     TPQKLLEKNVGQQVSIIRTNPSTGAETQETATVLSAQDGVVLKIGNRIETGIPGRIVY
                     QDVPGQLKDRPTLTTQVQYKGAEQSSVELSYLTGGLQWKADYVAELSSKEDAIDLSGW
                     VTLTNTSGTAYPNAKVQLVAGDVNRVREAIRPKTMMMRSEAMVADAAMPTEQGLLEYH
                     LYTLPRATTIAENQTKQVALLSAQHIPARKELVLAGADYYYQGQYRDIEKKQKVQVYV
                     EFENKESSKLGLPLPKGTMRVYKKDSDGTAQFVGEDLIDHTPKNETVRLKLGNAFDVT
                     AERVQTDFKNLSKQN"
BASE COUNT          982 a          943 c          866 g          750 t
ORIGIN      
        1 ctgacgatgt ggtgcatgca ttcgtcaaac aggctgctga tactggtgtt gatgtgttcc
       61 gtatctttga tgcaatgaac gacatccgca acctcaaaac ggctattgaa gccgttaagt
      121 ctgccaaaaa gcatgcaatt ggcaccctat ctttcaccac cagcccggtc catgacattg
      181 cctactttgt caacatggcg aaagagctag aagccttggg ttgcgacagc attggtgtca
      241 aagacatggc cggcttgtta accccaatct tggcaggtga cttggtaaaa gcattgaaag
      301 ccgctgtcag cgtgcctgtg cacatgcata gccatgctac ttcaggcctg gccagcatgg
      361 tgatgctcaa gagcgttgaa aacggtgtag atattatcga cacctgcaac tcatccttct
      421 ccgaaggtgc aagccacccc accactgaga gcctggtcgc agccttgaag ggcaccgaat
      481 acgacaccgg ccttgatttg ggcaaactgc aggaaatcac cgcttacttt aaagaagtac
      541 gtaagaaata ctggcagttt gaaagcgaat tcacaggtgt agacacccgc gtgctggtca
      601 accaggtacc aggcggcatg atttccaacc tgtccaacca gttgaaagag caaggcgcac
      661 tcaaccgtat ggacgaagtg ctggccgaaa ttcctcgtgt acgccaggac cttggctatc
      721 ctccgctagt cacaccaacc tcacaaattg ttggtaccca ggccgtattg aacgtattga
      781 ccggtggccg ttacaagtcc atcaccaacg aagtgaaaaa ctacctgttg ggccaatatg
      841 gtaaagcgcc tgctgcggta aatgcagatg ttcgtaaaca ggccgtgggt gacgcagaag
      901 tcatcacctg ccgtcccgca gatttgctgc aaccagaaat ggcgagattg cagtctgaag
      961 ccgagcgttt cgccaataac gaacaagacg tactgaccta cgccatgttc ccggatattg
     1021 gccagacctt cttgcaagag cgcaacgcag gctccttgca accagagcca ttgctgagca
     1081 aagaagcagt gacccagcaa actgccgcag cacgctacgc gcctaacgaa ttcaacatca
     1141 ccttgcacgg tgaaacctac catatcaaac tgacaggcag cggtcacgcc ggtgaagagc
     1201 gtaaaccata ctatgtcaaa gtagacggta tctccgaaga agtgtttgtc gaaaccttaa
     1261 gcgagatgga agttagcggt ggcggttcaa atggcagcaa gaagaaagcg gctgcagtcg
     1321 ccgccagtgg ccgcccacgc cccaaccacc caggctgcgt gacgacagcg atgcctggca
     1381 ccatcgtcac ggtcaaagtg aatgtgggtg ataaggtgaa tgcaggcgat ggtgtattgg
     1441 tcattgaagc catgaaaatg gaaaatgaga tccaggccag caaatcaggt actgtggtcg
     1501 caatccatgt ggccaaaggt gacagtgtca cccccgacga aacactgatt gaaattcagc
     1561 cagaataata tctcgccatt gaaatgctaa aatccccgaa tcactgttcg gggatttttt
     1621 atcctgatgc ctataccaag cttaattctc gcctcctctt cacgctaccg tcgtgaagta
     1681 ctagaaaaat tgcacttgcc gtttcaatgc gtctcaccag agattgatga aacgccatta
     1741 gcagatgaaa gcccggaaca aacctctctg cgcttggccg aaagtaaagc gcgcaaagta
     1801 gcacaaactt atcagaatgc actcattatc ggttgcgacc aggtcgctac ggttgacggg
     1861 ctgcaaattg gcaaaccggg caaccatgac aatgccgtca aacagctgac catgctcagt
     1921 ggcaaggaag tgattttcca tagtgccata tgcctgtaca acagcgccaa ccagcacatg
     1981 caatccacca tagcgcctta ttacgtcaaa ttcaaatccc tctcgcccgg gcagatcgaa
     2041 acctacctgc gcctggagca accctatgat tgtgcaggta gcgccaagtc tgaaggcatg
     2101 ggtattgccc tgctcgaata tatgcgcggt gacgacccaa atgcactgat aggcctgcca
     2161 gtgatcgtgc tggtcgatat gttgatgcag gcaggtgtag atgtattatc tgcatcacgt
     2221 acatagcgca acttaaagtc gagcgttaac ttttgaacgg ctgccacgtt aaggcagtct
     2281 caagcctata agcttacaaa caaaaacgag aggatattca tgcgactcag ctccctgctg
     2341 cttttttctt taggtagtgt ttccctgtgc agtacatcta tcgcacaagc agagatcacc
     2401 accgtcagcc cacaatccga ccaaaccagt gtggccgtga ctatctacaa tgaagatctt
     2461 gcactggtca aagacagccg aaaattgaac ctgagtagtg gtttatcagc cctgtcgttc
     2521 cgtgatgtca gtgcgcagat caagcctgaa actgccctgc tgcgtagcct gctctccaac
     2581 agcagcctgc aagtgctgga acagaatttt gactttgatt tgctgacacc acaaaaactg
     2641 ctggaaaaaa atgtggggca acaagtcagc atcatccgca ccaaccctag caccggcgcc
     2701 gaaacccagg aaaccgcgac tgtattgtcc gcgcaagatg gcgtagtcct taaaatcggc
     2761 aaccggattg aaaccggtat tcctggccgc atcgtttacc aggatgtacc tgggcagctt
     2821 aaagaccgcc ccacgctgac cacgcaagta caatacaaag gggctgaaca atccagtgtg
     2881 gaactcagct acctgaccgg tggcttgcaa tggaaagccg actacgtcgc agaactcagt
     2941 agcaaagagg atgcaattga cctctcaggc tgggtcacac tcaccaacac cagcggcacc
     3001 gcttacccca atgccaaagt gcaattggtc gcgggcgatg tgaaccgtgt acgtgaagcc
     3061 atccgcccca agaccatgat gatgcgctct gaagcgatgg tggccgatgc cgcaatgcca
     3121 actgaacaag gtctgcttga atatcacctg tataccctgc cacgtgctac tacgattgcc
     3181 gaaaaccaga ccaagcaagt cgccctgctt tctgcacagc atattccagc gcgtaaagag
     3241 ctggtattgg cgggtgcaga ttattactac caaggccagt atcgcgatat cgagaaaaag
     3301 caaaaagtgc aggtatatgt tgaatttgaa aacaaggaaa gtagcaagct gggcctgccg
     3361 ctccctaaag gcaccatgcg tgtttataaa aaggattctg acggcactgc gcaatttgtt
     3421 ggcgaagacc tgatcgacca tacgcctaaa aacgaaacgg tgcgccttaa gctcggcaac
     3481 gcttttgatg tgactgctga gcgcgtacaa actgatttca agaacctgag caagcaaaac
     3541 c
//
LOCUS       DATMSK010000214         6564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_86629,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000214 DATMSK010000000
VERSION     DATMSK010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86629"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VNZ84_07190"
     CDS             complement(<1..199)
                     /locus_tag="VNZ84_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU66778.1"
                     /translation="MTLQQMVGEQGFCLGNSEHAVLLVHGLTGTPAEMKHYGKQMARK
                     GLSVVCPLLAGHCASVNELGKT"
     gene            complement(199..588)
                     /locus_tag="VNZ84_07195"
     CDS             complement(199..588)
                     /locus_tag="VNZ84_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWU66779.1"
                     /translation="MTGMYSLFIAAVLLDTLGTTLFKLGASHAAQLETSLVPEVGLGF
                     GLRALQSFFSSWYIPFGLAVYVVEYVIWITYLSSSSLSRAFPMYSLTIVLILIVSKFV
                     LKEKVGLNRWLGALLIIAGVLMLGGSE"
     gene            complement(585..1457)
                     /locus_tag="VNZ84_07200"
     CDS             complement(585..1457)
                     /locus_tag="VNZ84_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HWU66780.1"
                     /translation="MEVVAYDFDGSLAMQQPLLQAYGIPLSVQSMRQFERSVRLWASL
                     PVFKKMAQSLRRPSAEGQFSLIGSGDYHHVSLALISQFTKPLTVVLFDNHPDWMRPPH
                     QFHCGTWVYSLARLAHVQRIVIVGLESGDIAGDQFRRGDMESYQLRKIVLLPYQPVQV
                     DPVATELKSSLATSLAVGIAEVLAEIETDNIYISVDKDCLRAADARTNWEQGSMPLST
                     VTAAIRALRAHKHVVGADTVGDFSPVRFRSPLKWIASYLDRREHPREEMVIASRINAA
                     ANAQLIGALTGKTS"
     gene            complement(1467..1772)
                     /locus_tag="VNZ84_07205"
     CDS             complement(1467..1772)
                     /locus_tag="VNZ84_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66781.1"
                     /translation="MQELKSRWKCVGDVSGLGLALRIELCESDGFTASRALADRMFNE
                     GLKGDVMIDGKPHGLVLDIGGYEKNVITLAPSLMISEQEVDLGIQLFEALLKRCGAE"
     gene            complement(1821..2840)
                     /locus_tag="VNZ84_07210"
     CDS             complement(1821..2840)
                     /locus_tag="VNZ84_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWU66782.1"
                     /translation="MDTKIIEILDKNNKYCSHGDTVNYADPPKVFESCDGSFLYDEKG
                     IRYLDWQMWYSAVNFGYKNERIANVLRKQIDTLPQLASQYLHKEKVELAEIICRYMEE
                     KYGVKGRVHFNVGGAQAVEDSLKVVRNHTGKTHQFAFMGGYHGRTLGASAITSSYRYR
                     RRFGNFADRAHFVPYPYCFRCPYDMKVETCDTYCHKEFEKLFDTEYQSFWDEKSKQCE
                     FGAFYIEPMQGTGGYIIPPDGYMEKLQASLKKRNVLLVADEIQMGVYRTGKLWSWENF
                     GIVPDIFVFGKAITNGLNPLSGIWAREELISPEKFPPGSTHSTFASNPLGTSVALEVL
                     KMTQE"
     gene            complement(2857..3525)
                     /locus_tag="VNZ84_07215"
     CDS             complement(2857..3525)
                     /locus_tag="VNZ84_07215"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HWU66783.1"
                     /translation="MLLIVDFDGTISKSDTIDALLEKFADDRWQQYEQDWLAGKIDAV
                     ECMSSQLALVKADNIALESFFRSIELDNSFRHFHAFAKQFAHVVIASDGLDHAIKVSM
                     QHSNFPELPVFANHLQFDPQGLQMTFPNRQPDCAGGNGNCKCAVAKAQRSFPAEKVVL
                     IGDGKSDACLAGRADVVFAKGKLVTHCVNHHIPFIPFSTFDDILATMRTWPESHVRFS
                     HQTA"
     gene            complement(3537..4409)
                     /locus_tag="VNZ84_07220"
     CDS             complement(3537..4409)
                     /locus_tag="VNZ84_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWU66784.1"
                     /translation="MQAAVKKPSAPRWIFAYSYWDIVPVLAGVLHFVCLIAFFVNFDH
                     LSWQWDVVFGLLYAVSISWNINGIAHNFIHNPYFKSEALNRLFSLMESITLFFSQTFY
                     DAVHKRHHIGNSDLPDAEGDTIDWLSIYRYGHDQQPENVWAYTFKSFFRDEPQKTYQE
                     IYKRNPFLARFGVFEIIVVVALVIAGLWINWKFVLFLAPFYYLGNSLSSLNGYYEHWR
                     GNPHLPIAWGVSCYNKLYNWLWFNNGYHAEHHYRPKHHWTQMKALHLQIAEQQKAAGV
                     HVIDWSHGLGFMQK"
     gene            4571..5245
                     /locus_tag="VNZ84_07225"
     CDS             4571..5245
                     /locus_tag="VNZ84_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU66785.1"
                     /translation="MRILLVEDDVVLGDALVQSLVNATYAVDWFRNGKEGMLAAQMQI
                     YDLVLLDLGLPFLNGFEFIQRLRNGKQPVPVIVLTANDNPEDIVRAFDTGADDYLIKP
                     FRLPELTARIRAQIRRVHTHFSSKIAIGELELDTKERMAFIKGEKLSLSPREFSLLET
                     LSIKFGKVVTKDALIESLCNWDQDIGTNAIEVYVHRLRKKLADCNVTIANIRGLGYMM
                     EQQTHG"
     gene            5238..>6564
                     /locus_tag="VNZ84_07230"
     CDS             5238..>6564
                     /locus_tag="VNZ84_07230"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU66786.1"
                     /translation="MASLFRSRGSDTLRKQLFLWVTIPMFVLIPLDTSILYKVGIHFV
                     NQSFDDSMEDMANDVVAMINESGRNPETFSMSKETEGVLFSNQYDKTYYAIYNATGKL
                     LSGNLPLPFRVKNLSKSKCFKSSVIQHERVRLLTVKSVHLGLQQKAQPYFVVIAETLH
                     KRHNLRSRILLAIVLPQIILMIVCGAMLLWSVTKGLEPLHELNEEIAKRSSTQLDPVM
                     LKNVPEEAMVLINSINLLMGRLRNAILAQNQFIADAAHQLRTPIAGIQAQVEVALKQE
                     RTRPLVMISESVFKLTHLVNQLLRLSHNQPEAANVIELVPVNLFTLAQETCAEVIHFA
                     LQKHIDLGFESSSRQLTPDFEVFGDSQRLKVMLLNLLDNAIRYTPDGGKVTLALTALE
                     DCIELRVEDNGVGIPENEHEMIFERFHRVLDNSQEGSGLGLSIVKEIAIL"
BASE COUNT         1728 a         1756 c         1539 g         1541 t
ORIGIN      
        1 tggttttgcc taactcattg acggacgcac aatgccccgc cagtaacggg cacaccaccg
       61 acaatccttt tcgcgccatt tgcttgccat aatgtttcat ttccgctggc gtaccggtca
      121 acccatgtac cagcaatacg gcatgttcgg agttccccag gcaaaagcct tgttcaccca
      181 ccatttgctg taacgtcatc actcactccc acccaacatc aacacaccag cgataatcag
      241 gagggctccc aaccagcggt ttaaaccgac tttttctttc aacacgaatt ttgagacgat
      301 caatatcagc acgatagtca ggctgtacat gggaaaagca cggctgagcg aagaggagga
      361 taaataggta atccagatca catattccac cacatagacc gccagtccga acggaatgta
      421 ccaactactg aaaaacgact gcaatgcgcg caagccaaat cccaaaccaa cctcaggcac
      481 cagtgaggtt tccagctgtg cggcatgact ggcgcccagc ttgaacaagg tggttcccag
      541 cgtatccagc agcacagcag caataaacag gctatacata ccggtcatga tgtctttcct
      601 gtcaacgccc caatcaactg cgcattggcc gctgcgttga tccggctggc aatcaccatt
      661 tcctcgcgag gatgttcacg cctatccagg taagaagcga tccacttcaa gggcgagcgg
      721 aaacgcaccg gtgaaaaatc ccctaccgta tccgccccta ccacatgctt gtgcgctctg
      781 agcgccctga tcgccgccgt gacagtactc aacggcatac tgccctgctc ccagttggtc
      841 ctggcatccg cagcgcgcag gcagtcctta tctacactga tgtagatatt gtctgtttct
      901 atctccgcca gcacctcggc aatccccaca gccaggcttg tcgccagact gctttttaac
      961 tccgtggcca cggggtctac ctgcaccggc tgatacggca acaacacaat ttttctcagc
     1021 tggtaactct ccatatctcc ccgtctaaac tggtcacccg caatgtcacc gctctccaga
     1081 ccgacaatca caatacgctg cacatgcgcc aacctggcca ggctgtaaac ccaggtccca
     1141 caatgaaatt gatgcggcgg ccgcatccaa tccggatgat tatcaaaaag caccaccgtc
     1201 aacggcttgg taaactgtga aatcagtgcc agggaaacat ggtggtaatc ccctgaaccg
     1261 atcagactga attgcccctc ggctgaaggc ctgcgtaatg actgggccat ttttttaaac
     1321 actggcaaag aagcccacaa gcgcacagac cgctcgaatt ggcgcataga ctgaaccgac
     1381 agcggaatac cataggcctg cagcaaaggc tgctgcatgg ccaggctacc gtcaaagtcg
     1441 taggcgacaa cttccatgat ggcgacttat tcagccccgc aacgtttgag cagcgcctca
     1501 aacagttgta tgcccagatc aacttcctgt tcggaaatca ttaacgaagg cgccagcgta
     1561 atcacattct tttcatagcc gccgatatcc agcactaggc catgcggctt gccatcaatc
     1621 atcacgtcgc ctttcaggcc ctcattgaac atacggtcgg ccaaggcgcg gctggcggta
     1681 aagccatcgc tctcgcacaa ctcgatacgc aatgccaggc ccaggccgct aacatccccc
     1741 acgcatttcc agcgtgattt cagttcctgc aagcgcttga ggaaataagc accgcgctca
     1801 cgtacatggg gctcaaagtc ttattcctgc gtcattttca ggacttccag tgccacagaa
     1861 gtgcccaacg gattggaagc aaaagtagag tgggtagaac ctggcgggaa tttttccggt
     1921 gaaatcaact cttcgcgcgc ccagatccca gacagcggat tcaaaccatt ggtaatcgct
     1981 ttaccaaaca cgaagatgtc aggcacgatg ccaaagtttt cccatgacca taacttaccg
     2041 gtacggtaga cccccatctg gatttcatcc gccaccagca acacattgcg ttttttcagt
     2101 gaggcttgca gtttttccat atagccatca ggcgggataa tgtaaccacc ggtgccttgc
     2161 ataggctcaa tataaaacgc cccaaattcg cactgtttgg atttttcatc ccagaatgac
     2221 tggtactcgg tatcaaacag tttttcaaac tctttgtggc aataagtgtc gcaagtctcc
     2281 accttcatgt cgtaaggaca acggaagcaa tatggatatg gcacaaagtg cgcgcggtcc
     2341 gcaaagttgc caaaccggcg acgatagcga taactggaag taatggcaga cgcccccagc
     2401 gtccggccat ggtagccgcc cataaaagca aactggtggg ttttaccggt gtggttacgc
     2461 accaccttga gtgaatcttc aaccgcctga gcaccaccga cgttgaaatg cacacggcct
     2521 ttaacgccgt atttttcttc catatagcgg cagatgattt ctgccagctc tactttttcc
     2581 ttatgcaggt actggctggc cagctgcggc aaagtgtcaa tctgcttcct cagtacattg
     2641 gcaatacgct cattcttgta gccaaaattc acggccgaat accacatctg ccagtccagg
     2701 taacgaatgc ctttttcgtc gtacaggaaa ctgccatcgc aactttcaaa tactttggga
     2761 ggatccgcgt agttgacggt atctccgtgt gaacaatact tgttgttttt atcaaggatt
     2821 tcaataattt tcgtgtccat actaacgcga cctcagttaa gcagtttggt gagaaaaacg
     2881 cacatgactt tcaggccatg tcctcatcgt ggccagaatg tcatcaaacg tagaaaacgg
     2941 aataaacggg atatggtgat tcacgcaatg cgtcaccagc ttgcctttgg caaagaccac
     3001 gtcggcccgc ccggccaggc aagcgtcaga tttaccatcg ccaatcagca cgactttttc
     3061 agccgggaat gaacgctgcg cttttgccac ggcacatttg caattgccat taccgcctgc
     3121 acaatccggc tgtcggttgg gaaatgtcat ctgcaggccc tgcggatcaa attgcaggtg
     3181 attggcaaat acgggtaact ccggaaaatt ggaatgctgc atggatactt tgatcgcgtg
     3241 gtccaggcca tcgcttgcaa tcaccacatg ggcaaactgt ttggcaaacg catgaaaatg
     3301 acgaaagctg ttatccagtt caatcgaacg gaagaagctt tccagcgcaa tgttatccgc
     3361 cttgaccaaa gccagctgac tgctcatgca ctcaacggca tctatcttgc cggctagcca
     3421 gtcttgctca tattgctgcc agcggtcatc agcaaatttt tccagcaagg catctatggt
     3481 gtcactcttg gaaatggtgc catcaaaatc gacaatcaat aacatttacc ccccctttat
     3541 ttctgcataa agccgaggcc atgtgaccag tcgatcacat gtacaccagc ggctttttgt
     3601 tgttcggcaa tttgcagatg caaggctttc atctgcgtcc agtgatgctt gggccggtag
     3661 tgatgctcgg cgtgataacc gttgttaaac cagagccagt tgtagagttt gttatagcaa
     3721 ctcacgcccc aggcaatcgg caaatgagga tttccacgcc agtgctcgta atagccattc
     3781 agggaagaca ggctgttgcc caagtagtaa aacggtgcca ggaacagtac gaatttccag
     3841 ttgatccaga ggccagcgat gacgagtgcc acgacgacaa taatctcgaa cacaccaaac
     3901 ctggccagga aaggattgcg tttgtatatc tcctggtaag ttttttgagg ctcatcgcgg
     3961 aagaaactct tgaaggtata agcccacaca ttttcaggct gttggtcatg cccataacga
     4021 tagatggata accagtctat ggtgtcgcct tcagcatcgg gcaaatcact gttaccaatg
     4081 tgatggcgct tatgtacggc atcataaaaa gtctgggaga agaacagcgt gatggactcc
     4141 atcaggctga acaggcggtt cagtgcttca gacttgaaat aagggttatg aataaaatta
     4201 tgtgcaatgc cattgatgtt ccaggaaatg ctgacggcgt atagcaagcc gaagacgaca
     4261 tcccattgcc aggacaagtg atcgaaattc acaaagaaag cgatcaggca aacaaagtgc
     4321 aaaacacccg ccagtacagg gactatatcc cagtatgagt aggcaaaaat ccagcggggc
     4381 gcgcttggtt ttttgacagc tgcttgcatg aagttctcct gaaagtggat acgcaatgaa
     4441 tcgcgtgtgc ccatcatgca gccaaattct tactaaacac ttacagtcat ttttgtttca
     4501 tctggctgta agtaatctgt aagtcagcct gatgcaagct agcgaacatc cctctttggg
     4561 caaataatcc atgcgtatac ttttagtaga agatgatgtc gtactgggcg atgccttggt
     4621 gcaatccctg gtcaacgcga cctatgccgt tgactggttt cgcaatggca aagaaggcat
     4681 gctggctgcc caaatgcaaa tctatgacct tgtcctgctg gatctgggct tgcccttttt
     4741 aaacggattt gagtttatcc agcgcttgcg caatggcaaa caaccggtgc ctgtcatcgt
     4801 gctgactgcg aatgacaacc ctgaagatat tgtgcgtgcc tttgataccg gggcggatga
     4861 ttacctgatc aagccattca ggttgcctga actgacggcc cgcatccggg cacaaatcag
     4921 gcgggtacat acccactttt cttccaaaat agccatcggt gagttggagc tggatactaa
     4981 ggagcgcatg gcatttatca agggtgaaaa gctctccctg tcaccgcggg agttttcact
     5041 gttggaaacc ttgtctatca agtttggcaa ggtggtgacc aaagatgcgc tgattgaaag
     5101 cctgtgcaat tgggatcaag acatcggcac caatgccatt gaggtctatg tgcaccgtct
     5161 gcgtaaaaaa ctggccgact gtaatgtcac catcgccaat atcaggggct tgggctacat
     5221 gatggaacag caaacccatg gctagtttat tccgctcccg cgggagtgat accttacgca
     5281 aacaactgtt cctgtgggtc accattccca tgtttgtgct gattcccctg gatacctcta
     5341 tcctttacaa ggtgggcatc cactttgtga accagtcatt tgatgacagc atggaagata
     5401 tggcaaacga tgtggtggcc atgattaacg aatccggtag gaacccggaa acgttcagca
     5461 tgagtaaaga aaccgaaggg gtattgtttt ccaaccagta cgacaaaacc tattacgcca
     5521 tttacaacgc taccggaaag ctgcttagcg gcaacctgcc cctccccttc cgggtcaaaa
     5581 acctgtctaa aagcaaatgc tttaaatcct cggtcatcca gcatgaacgt gtacggttac
     5641 tgacggtaaa aagtgttcac ctgggactac aacaaaaagc ccagccctac tttgtcgtga
     5701 tcgcagagac cctgcacaaa cgccataatt tacgtagccg cattttactg gcgatcgtat
     5761 tgccacagat catattgatg atcgtgtgtg gcgccatgct gctctggagc gtgaccaaag
     5821 gcctggaacc cctccatgaa ctgaatgagg aaatcgccaa acgatccagt acccagctcg
     5881 acccggtcat gctcaaaaat gtccctgaag aagccatggt cctgatcaac tcaatcaatt
     5941 tgctcatggg gcgtttacgc aacgccatcc tggcgcagaa tcaatttatt gccgatgccg
     6001 cacaccagtt gagaacgccg attgcgggca tacaagcaca ggtggaagtc gcccttaaac
     6061 aagaacggac gcgtccgctg gtgatgatca gcgagagcgt gtttaaactg acccacctgg
     6121 tcaaccagtt gttgcgactg tcccacaatc agccagaagc tgccaatgtg atcgaactgg
     6181 tacccgtcaa cctgtttacg ctggcccagg aaacctgtgc cgaagtgatt cattttgcat
     6241 tacaaaaaca cattgacctt ggttttgagt ccagctcacg ccaactcacc ccggactttg
     6301 aggtgtttgg tgacagccag cgtttaaaag tcatgctgct taatttactg gataacgcaa
     6361 tcaggtatac gccagatggc ggcaaagtga cactcgcact gaccgcattg gaagactgta
     6421 ttgaattacg ggtggaggat aacggggtcg ggatcccgga aaatgaacat gaaatgattt
     6481 ttgaaagatt tcatcgtgta ctcgacaact cacaggaagg cagtggtttg ggattatcca
     6541 ttgtgaaaga aatcgccatc ttgc
//
LOCUS       DATMSK010000215         4679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_416442,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000215 DATMSK010000000
VERSION     DATMSK010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416442"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /gene="prs"
                     /locus_tag="VNZ84_07235"
     CDS             <1..496
                     /gene="prs"
                     /locus_tag="VNZ84_07235"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HWU66787.1"
                     /translation="ILLDDLAQQGHENLVVVSPDVGGVVRARAAAKQLNADLAIIDKR
                     RPKANVAKVMNIIGEVEGRTCVIMDDMVDTANTLCEAAAALKDRGATKVVAYATHPVL
                     SGGAGERITASALDELVVTNTIALNAGAQACSKIRQLSAAPLLAETIKRISQEESVSS
                     LFMD"
     gene            608..1222
                     /locus_tag="VNZ84_07240"
     CDS             608..1222
                     /locus_tag="VNZ84_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HWU66788.1"
                     /translation="MSIEIKAVKRDVKGTGASRRLRRAGSVPGVVYGGDKAAVSIELD
                     HKELFMEFRHEAFHASILSLVLDGKAESVLLRDYQLHPVRNTIQHIDFQRVSATEKIH
                     VKVPFHFINEDVAPGVKTGGGNISHIQTEADVSCLAKDLPEFIEVDLAKLEAGHSIHL
                     SEIKLPKGIEFVQLAHGDDAAVVSIAKPRGGSGDEEAAEETPAA"
     gene            1303..1875
                     /gene="pth"
                     /locus_tag="VNZ84_07245"
     CDS             1303..1875
                     /gene="pth"
                     /locus_tag="VNZ84_07245"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWU66789.1"
                     /translation="MTGIKLIVGLGNPGDKYTMTRHNAGFWFVDLLAQQSNSRLAAEA
                     KLFGIAGKWLPHSDKWLLKPSTFMNASGKSVAATANYYKISPAEILVVHDELDLPPGQ
                     AKLKFGGGHGGHNGLRDIHAALGTADYWRLRLGIGHPGNKAEVVNFVLKAAGKDEQQA
                     IDDSIIKATTVSDLLAQGQFEKAMLQLHSK"
     gene            complement(1943..>4679)
                     /locus_tag="VNZ84_07250"
     CDS             complement(1943..>4679)
                     /locus_tag="VNZ84_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU66790.1"
                     /translation="LRSSRWMAVVVLCCALQATFLLWMSERHHAEALQAVKFKQSVLS
                     ASEGIKQRLNAYRQILKGVEQLYISSEFVSADEFRLYVEDFTQSPEYNSLSALGFIKY
                     IHLHHPETFKDLEMPIQQLLKQMEFVKGDDEMAPVLYVEPRNDANREVLLKNAFLDGQ
                     LRADLLEAGDGDRLVMSRHASMNSELHPYKNYLFNAPVYRSRPDAKPGERRARLNGWV
                     FLRFDVNTMLAEALRPIEQRLIHFDVFDLGRVEGQVPLYHSGGSEGAHVHDTEPAWST
                     ASTLNLNGQVWRLQARSTPAFEAATDYRNANDVGILGVVISLLLAGMVQFFVIRNRTR
                     HALETYSRALSSSEQRWKVAMESTGDGVWDWNVTEARIVFSEHWQKILGFAPQKGDHK
                     PDAWHQRIHPDDVPEVTALQQQVLDGEREQYAIEYRLLCPDGSWKWVFDRGRVLMHDE
                     HGKPTRILGTLADISKIKQSEEVVWQYANVDTLTGLPNRRLFLDRLEQGLQAIKGNGQ
                     KLAVVFLDLDRFKEVNDTQGHDQGDMLLLQAGKRLAGCVGNKDLVARLGGDEFVLMLS
                     DATAGYVEALAQRVLDALSQPFQLDETHAYVAASLGIAIYPDDAANKDDLMKRVDQAM
                     YASKQKGGNCFTYFTPRMQQNAEQRMRLSHDLRQAINHEEFFLEYQPVVNLQTNMVVK
                     AEALLRWQHPVKGLIPPMEFICIAEDNLLIIPIGEWVFKTAIEQCRQWRQSLHPLFQM
                     AINKSPVQFATEYRKQDDWLSKVSHGYYEGNMVVVEITERLLLDGNSQISERLSQYQQ
                     AGVQVALDDFGTGYSALSYLKKFPIDYVKIDRSFVSELGTSSEDEALCRAIIVMAHSL
                     GMQVIAEGIENQVQLSMLQEMGCDFGQGYYFSPPLRPEAFVRWHQEWHQKSVLGR"
BASE COUNT         1155 a         1321 c         1115 g         1088 t
ORIGIN      
        1 gatcttgctc gacgacctgg ctcaacaagg tcacgagaac ctggtggtgg tttcaccaga
       61 cgtaggtggc gtggtgcgtg cccgtgcggc agccaaacaa ctgaatgcag acctggcgat
      121 tatcgacaaa cgtcgtccaa aagccaacgt cgcaaaagtc atgaacatta ttggtgaggt
      181 cgaaggccgc acctgcgtca tcatggatga catggtggat accgcaaata ccctatgtga
      241 agcggctgca gccttgaaag accgtggcgc gactaaagtg gttgcctacg caacccatcc
      301 ggtattatcc ggcggcgctg gggagcgaat taccgcttcc gcactggatg agttggtcgt
      361 caccaacacc atcgcattaa atgctggcgc acaggcctgc agcaaaatcc gccaactgag
      421 tgcggcacca ttgctggcag agaccatcaa gcgcatcagc caggaagaat ccgtttcttc
      481 cttatttatg gattaatcca tcaatataaa gttagaaagc atggattaag ttttacacca
      541 tctgctggtc gcggcaggtg gattttagtg cagtaccgcc atttatggcg tttttaggag
      601 tctcaacatg agtatcgaaa ttaaagccgt caaacgtgac gttaaaggta cgggtgcgag
      661 ccgccgcctg cgtcgcgctg gcagcgtgcc tggtgttgtt tacggtggcg acaaagctgc
      721 tgtctcaatc gagttggatc acaaagagct gttcatggaa ttccgccatg aagctttcca
      781 cgcttctatc ctgagcctgg tgctggatgg caaagcagaa agcgttttgc tgcgtgacta
      841 tcaattgcac ccagtacgta acactattca gcacatcgat ttccagcgcg tttcagcgac
      901 tgagaaaatc cacgttaaag tgcctttcca cttcatcaat gaagatgtgg ctcctggcgt
      961 gaaaacaggc ggtggtaaca tctcccacat ccagactgaa gctgacgtaa gctgcctggc
     1021 aaaagatttg ccagagttta tcgaagtgga tctggctaaa ctggaagctg gtcactctat
     1081 ccacttgtcc gaaatcaagc tgccaaaagg cattgagttc gtacaactgg cacacggtga
     1141 cgacgctgcc gtggtttcta tcgccaaacc acgtggtggt agcggcgacg aggaagctgc
     1201 agaagaaact ccagcagcct aagcaggccc ggcggttatc cgctacaata aacgcgcact
     1261 cttcagtgcg cgttttttct ttgtctgaca caaatacaca ccatgactgg cattaaactg
     1321 attgtaggct tgggcaaccc cggcgacaaa tacaccatga cccggcataa cgcgggtttc
     1381 tggtttgtgg atttactggc acaacaatcc aatagccgcc tggcggcaga agccaaactg
     1441 tttgggattg ccggtaagtg gttgccacat agtgacaaat ggctgctcaa gccgagcacc
     1501 tttatgaacg ccagcggcaa atccgtggca gccaccgcca attactacaa gatttccccc
     1561 gccgaaattc tggtggtaca tgatgaactg gacctgccac ccggccaggc caaactcaag
     1621 tttggtggcg gtcatggcgg tcataacggc ttgcgggata tccatgccgc actgggcacc
     1681 gcagattact ggcgcttgcg cctaggcatc ggtcatccag gcaacaaggc agaagtggtc
     1741 aattttgtct tgaaagctgc gggcaaagac gagcagcaag ccattgatga cagcatcatc
     1801 aaggctacca ctgtcagtga tttactggcc caggggcaat ttgaaaaagc catgttgcaa
     1861 ctgcatagca aataaaaaag ggcgcaacaa cgccctggtg tttttagccc ttttaaaaca
     1921 atggagctct ctggttttat gctcagcgcc ccagaacaga cttctggtgc cactcctgat
     1981 gccagcgtac aaatgcttcc ggccgcaacg gtggcgaaaa ataatacccc tgaccaaagt
     2041 cgcagcccat ctcctgcaac atactcaatt ggacctggtt ctctatcccc tctgcaatca
     2101 cctgcatgcc caagctatgc gccatgacaa taatggcacg gcataaagcc tcatcttcgc
     2161 tagacgtgcc tagctcactg acaaaagagc ggtcaatctt gacgtaatca atcgggaatt
     2221 ttttgaggta agacaaggcc gagtatccgg tgccaaagtc gtccaaggcc acttgcaccc
     2281 ctgcctgctg atattggctc aggcgttcac tgatttgact gttgccatcc agcaataagc
     2341 gctcggtaat ttccaccacc accatattgc cttcatagta cccatgagag actttcgata
     2401 accagtcatc ttgttttctg tactcggtgg caaactgcac cggcgactta ttgatcgcca
     2461 tctggaacag tggatgcaag gactgacgcc actgacgaca ttgttcaatc gccgttttga
     2521 atacccactc gccaatagga ataatcagca agttatcttc tgctatgcag ataaactcca
     2581 taggcgggat caaacccttg accggatgct gccagcgcag caaggcttcc gctttcacca
     2641 ccatattggt ctgtaaattc actaccggct gatattcaag gaaaaattct tcatgattaa
     2701 tcgcctgccg caaatcatgt gacaggcgca tacgttgctc tgcgttctgc tgcatgcggg
     2761 gtgtaaagta ggtgaaacag ttgccgcctt tttgcttgga agcatacatc gcctggtcga
     2821 cgcgcttcat caagtcatcc ttgttcgccg catcatccgg gtaaatggca atccccaggc
     2881 tggccgccac ataggcatgt gtctcatcca gttgaaaagg ctgggataac gcatcgagca
     2941 ctcgctgcgc caacgcttca acatacccgg ccgtggcatc actcagcatc aacacaaact
     3001 cgtcaccccc cagcctggcc acaaggtctt tgttgcccac acacccggcc agccgcttgc
     3061 cagcctgcag caataacatg tcaccctggt cgtgcccttg cgtatcgttt acttctttaa
     3121 agcggtccaa atccaggaaa acgacagcca gtttctggcc attgccttta atcgcttgca
     3181 gtccctgctc cagccggtca agaaacaggc gccggttggg taaaccggtc aatgtatcca
     3241 cgttggcata ttgccaaacc acctcttctg actgcttgat tttggagata tcggccagcg
     3301 tgcccaatat ccttgtcggc ttgccgtgtt catcgtgcat caataccctg ccacggtcga
     3361 acacccattt ccagctacca tccgggcaga gcaagcggta ttcaatcgca tactgttctc
     3421 tctcgccatc cagtacctgc tgctgcaacg ccgtcacttc aggcacatca tcaggatgga
     3481 tacgctgatg ccaggcatca ggtttatggt cacccttctg cggcgcaaaa cccaaaatct
     3541 tttgccaatg ttcggaaaaa acaattctgg cctcggtaac attccagtcc catacaccgt
     3601 cacccgtgct ttccatcgcc actttccagc gctgttcact gctactcagc gcccggctat
     3661 atgtttcgag tgcgtgtcgc gtgcggttcc gtatcacaaa aaactgtacc atccccgcca
     3721 acaataggct gatgaccaca cccaatatcc ctacatcatt ggcattacgg taatcggtag
     3781 ccgcctcaaa tgccggggtt gaacgcgcct gcaaccgcca tacctggcca ttcagattca
     3841 gggtagaggc ggtactccac gctggttccg tgtcatgcac gtgcgcccct tcactccccc
     3901 cgctgtgata caagggcacc tgcccctcaa cacgccccag gtcgaatacg tcaaaatgga
     3961 tcagccgctg ctctatcggg cgcagggctt ctgccaacat ggtgttgaca tcaaaacgta
     4021 aaaataccca gccattcaag cgggctctgc gctctccagg tttcgcatca ggcctgctgc
     4081 gatataccgg tgcattaaaa agataatttt tgtacggatg caactcgcta ttcattgagg
     4141 catgacgtga cattaccagg cggtcaccat cgcccgcttc cagcaaatcg gcgcgcaact
     4201 gcccatccag gaatgcgttc ttgagcaaga cctcccgatt ggcgtcattg cgtggctcaa
     4261 catagaggac aggcgccatc tcatcatcgc ctttgacaaa ctccatctgt ttgagcaact
     4321 gttggatagg catctccagg tctttaaacg tctctgggtg atgcagatga atgtacttga
     4381 taaatcccag cgcactcagg ctgttatatt caggactttg ggtaaagtcc tccacataca
     4441 acctgaactc atccgccgag acaaactccg aggaaatata aagttgttct acgcctttta
     4501 atatttggcg ataagcattc aggcgttgct tgatgccttc agacgcagac agtacggact
     4561 gcttgaattt aaccgcttgt agcgcctcgg catggtgccg ctcactcatc cacagcaaaa
     4621 aagtggcttg taatgcgcaa cacaagacca cgacggccat ccatcgcgac gaccgtagc
//
LOCUS       DATMSK010000216         5975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_416464,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000216 DATMSK010000000
VERSION     DATMSK010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416464"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VNZ84_07255"
     CDS             <1..1112
                     /locus_tag="VNZ84_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWU66791.1"
                     /translation="AGGSAKQGRDRKFQAILPLKGKILNVEKARFDKLLGSQEIATLI
                     TAMGTGIGKDEFKLEKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVENGHIYI
                     AQPPLYKVKQGRDERYLKDEHELDEYLLNSALRDAVLVTKEGGDILKDEALVSIAKQM
                     VLTEAVIRRIANLYDESVLRAIQDLGDLDLSTESAAKTAAEKLRPILGAAGSEVIVAQ
                     DTETSAYRLEVNKYVHGNLQSCVIDAEFLSSGDYRQINRTSQMVNNLLGEGAMIKRGE
                     KEQKVSTFKQALDWLLGEAKHNLNIQRYKGLGEMNPEQLWETTMDPTVRRLLKVQIED
                     AIGADEIFTTLMGDNVEPRRAFIESNALSVSSLDV"
     gene            complement(1208..2083)
                     /locus_tag="VNZ84_07260"
     CDS             complement(1208..2083)
                     /locus_tag="VNZ84_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU66792.1"
                     /translation="MTRKNIISQNWTKAVCRANGINIHYLRTGGEKPTLIALHGLTGS
                     GACWSQLARELEPHYDIIMPDARGHGMSSAPQHGYTYQDHANDVIELIRTLDIKNPYL
                     LGHSMGGMTAAMVASRLGFAISGIILADPTFISPEWQDQVYESGIVEQHRQMLKSTKE
                     ALFKEAKAKHRFRSSELLEHIVEARLQTSVKAFDVLIPPNPEFEELIRNIYVPTLLVL
                     GSEGIVSSETARELHEINPDLQYKLIPDVGHGLPYDEPVRLAAAIDAFLKPTFKTKQR
                     MKTPSKFSVYTTNAF"
     gene            complement(2086..2706)
                     /locus_tag="VNZ84_07265"
     CDS             complement(2086..2706)
                     /locus_tag="VNZ84_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HWU66793.1"
                     /translation="MSEEIKKERIRQPGGGRKAGTGLYKEETSVIRVPNSQKPVITSF
                     LEAYAKKQRLSELKDNLDSIEEFVQLSEALESISLPLYQSKVPAGLPSPADSDVDSRM
                     DPNEYLINKADKTFFVTIQGESMIEVGLMPGDKAIVEKDKIPAVGDIVLAMIDGEFTV
                     KILAKQKDGNPKLLPANSSGKYSPILIQGQMQFAIWGVVTGSFRRF"
     gene            2814..4052
                     /locus_tag="VNZ84_07270"
     CDS             2814..4052
                     /locus_tag="VNZ84_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HWU66794.1"
                     /translation="MQLKQKLEILADAAKYDASCASSGTDKRTSSDGKGIGSTTGMGI
                     CHSYAPDGRCISLLKILLTNFCVYDCIYCVNRVSSNVPRARFSVDEVVNLTLSFYRRN
                     YIEGLFLSSGIIRNPDYTMEQVVEVARVLREVHDFRGYIHLKTIPDASPELMARAGLY
                     ADRLSINVELPTISSLQTLAPEKDGQAIRRSMGQLRLKIEESKDKTLTGKKPKRFAPG
                     GQSTQMIVGADATDDRAILDTSANLYGAYRLRRVYYSAFSPIPDASKMLPLKAPPLVR
                     EHRLYQADWLLRFYGFEADEIVTGHDGMLDLDVDPKTAWALQHREQFPVDLNRASKAM
                     LLRVPGLGVTSIARILAARKVRAIRYDDLARLRVSLKKVLPFILLPDYHPGHRLDDQH
                     LKQRLQRTIPPQQFGLFESV"
     gene            4039..5520
                     /locus_tag="VNZ84_07275"
     CDS             4039..5520
                     /locus_tag="VNZ84_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HWU66795.1"
                     /translation="MSPSSMVLVRLKNETDFDGWRTAARSLLAQGIAPEAVDWRVSDT
                     SPGLFDTPEFVPAHIPGWAFSVSRDFFQLCKTAILYRDPMRFGLLYRLLWRLHEVPEL
                     LQMSFDPDVIKARQMAQAVRRDLHKMKAFVRFREIKMQDQEPHFVAWFEPSHHIVEAS
                     AAFFSGRFTNMRWSILTPEICMHWDSHQLHYSPGASKADAPAEDADEVLWQSYYRSIF
                     NPARLKVSAMLSEMPKKYWRNLPEAALIADLIANADQRKDMMIAAAATEPTRKIVKYL
                     PDKEGDGMRAEAVGAQAALYRLNEEMLAHGAFELAAYATQAVMGSGAVPSSILLLGDQ
                     PGEQDDLAGQPLTGPVGTLLDKALNARGVGRSNVYVTNVLKHYAFRLQGTRRVARAPE
                     AREIQAYLPWLRAEIAIVEPSVIVALGPVAARALTGRAMDMEAYRGKRLSLTDGRQVV
                     VTYHPAMILRIADQATKQLRYAQLLNDLTLAAKVATQCVFEKS"
     gene            complement(5542..5772)
                     /locus_tag="VNZ84_07280"
     CDS             complement(5542..5772)
                     /locus_tag="VNZ84_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66796.1"
                     /translation="MKIEFKKSELQANLDVVTINDAIVGEFITDVSNQSFLFVPAKGD
                     RILLSRVTYEEACKLLGEELTQLYAHRCAMAA"
BASE COUNT         1479 a         1477 c         1534 g         1485 t
ORIGIN      
        1 cagcgggtgg ttctgccaaa caaggccggg accgcaagtt ccaggcgatt ttgccgctca
       61 aaggtaaaat ccttaacgta gaaaaagcgc gttttgataa actgctaggc tctcaggaaa
      121 tcgccacgct gattaccgcc atgggtactg gcattggcaa agacgagttt aagctggaaa
      181 aattgcgtta tcaccgcatc atcatcatga cagatgctga cgtcgatggc gcgcacatcc
      241 gtaccttatt gctgacgttc ttctaccgcc agatgccaga gctggttgag aatggccata
      301 tctatattgc gcaaccacca ttgtacaaag ttaaacaggg ccgtgatgag cgttatctga
      361 aggatgagca tgagctggat gagtatctgc tgaactcagc actgcgagat gccgtgctgg
      421 tgaccaaaga gggtggcgat attcttaaag atgaagcgct agtcagcatt gccaaacaaa
      481 tggtattgac tgaagccgtt attcgccgta tcgccaacct gtatgacgaa agcgttctac
      541 gtgcaatcca ggatttaggt gaccttgatc tgagtacaga atcagctgcc aagacagcgg
      601 ccgaaaaact gcgtcctatt ctgggagcag cgggctcaga agtgattgtg gcgcaagata
      661 ctgagaccag tgcttaccgc ctcgaagtca ataaatatgt gcacggtaat ctgcaaagct
      721 gtgtcattga tgccgagttc ctgagcagtg gcgattaccg ccagatcaac cgcacctcac
      781 agatggtgaa taacctgtta ggtgaaggcg cgatgatcaa gcgcggtgaa aaagagcaaa
      841 aagtcagcac ttttaaacag gcgctagact ggttgctggg tgaagccaaa cataacctga
      901 atatccagcg ctataaaggg ttgggcgaaa tgaaccctga gcagctgtgg gaaaccacca
      961 tggatcctac tgtacgccgc ttgcttaaag tgcaaattga agatgccatt ggtgcagatg
     1021 agatattcac cacattgatg ggcgataatg tggagccaag gcgtgcattt attgaaagta
     1081 acgcactcag tgtcagctca ctggatgtat agttttccat aggattattt aatgcttcaa
     1141 taataaaaaa ggtcccgaag cgggaccttt tttatatcat tcatccgact atttagcctc
     1201 gaaacattta aaaagcattt gtcgtgtaaa cagaaaactt tgacggtgtt ttcatgcgct
     1261 gtttagtttt gaatgtgggt ttcaggaaag catcaatagc agcggccaaa cgcacaggtt
     1321 catcatatgg caagccatgc ccaacatccg ggatgagttt gtactgcaga tctggattaa
     1381 tctcgtgcaa ttcacgcgcc gtttcagacg aaacgatgcc ttcactccct aataccagta
     1441 gggttggaac ataaatattc cgaatcagct cctcaaattc agggttaggc ggaatcagca
     1501 catcaaacgc tttcacactg gtttgcagcc tcgcttcaac aatatgttca agcaactcag
     1561 atgaacggaa acgatgtttt gccttagctt ctttaaacaa cgcttccttg gtagatttaa
     1621 gcatctgccg atgttgttct acgatgccgc tttcgtaaac ttgatcctgc cattccgggc
     1681 tgatgaaggt tgggtcagcc aggataatac cgctaatcgc aaagcctaac cggctggcta
     1741 ccatggctgc agtcatgccg cccatcgagt gccccagcaa atacggattt ttaatatcga
     1801 gtgttctgat cagctcaatc acatcatttg catggtcctg gtaggtgtat ccatgctgtg
     1861 gcgcactcga catgccatga cctctggcgt ccggcatgat gatgtcatag tgtggctcca
     1921 gttcgcgtgc caattgcgac cagcaggcgc cactgcctgt taagccatgc aaggcaatta
     1981 aagtgggttt ttcaccgcca gttctcaaat agtgaatatt gatcccgttg gcgcgacaaa
     2041 ccgcttttgt ccagttttgc gaaataatgt ttttacgtgt catgattaaa agcgcctgaa
     2101 agaacctgtg acgacacccc agatggcgaa ttgcatctgg ccctgaatga ggatgggtga
     2161 atatttgcct gaagagttag ccggtaataa ttttgggttg ccgtcttttt gtttagccag
     2221 gattttgaca gtgaactcgc catcaatcat ggccagcaca atatcgccca ctgccggaat
     2281 cttgtctttt tccacaatgg ctttatcgcc tggcatcagg cccacctcaa tcatggattc
     2341 cccttggatc gtgacaaaaa aagttttgtc cgctttatta atcaggtatt cattgggatc
     2401 catgcgcgaa tcaacatcgc tgtcagcggg agatggcaaa ccggcaggca ccttggactg
     2461 ataaagaggc aagctgatgc tttctagcgc ttcagataac tgaacaaact cttcaatgct
     2521 atccaggttg tctttcaact cactcaggcg ctgcttcttt gcataggctt ccagaaagct
     2581 ggtgattaca ggcttttggc tgttaggcac gcggatgacg ctggtctctt ccttgtagag
     2641 accagtccct gctttacgcc cgccacctgg ttgccggata cgctcttttt taatttcttc
     2701 gctcatgtta tttgtgtaac aaaaataatg ttattatcgt aacagaaata aatgaaagat
     2761 ttctactcct ttttgaattt attttctata tttatttagc caaaaaactc cacatgcaac
     2821 tcaagcagaa acttgaaata ctggcggatg ctgcaaaata cgatgcctca tgcgcttcta
     2881 gtggtacgga caaacgtact tccagcgacg gcaaaggcat aggctcgacc acaggcatgg
     2941 gcatctgcca tagctacgca cctgacgggc gctgtatttc attgctgaaa atattgctga
     3001 ctaatttctg cgtgtatgac tgtatctatt gtgttaatcg cgtcagcagc aatgtgccgc
     3061 gcgcgcggtt cagtgtggat gaggtggtga acctgacatt gagcttttac cgccgcaatt
     3121 atattgaagg gctgttcctg agttcaggca ttattcgcaa tcctgactac accatggagc
     3181 aggtggtaga ggtcgctcgc gtattgcgcg aggtgcatga tttccgtggt tacatccacc
     3241 tcaagaccat tcccgatgcc agcccggaac tgatggcacg tgctggcctg tatgctgacc
     3301 gactgagcat caatgtggag ttgccgacca ttagcagcct gcagacactg gcgccggaga
     3361 aagatgggca ggccatccgc cgttccatgg ggcaattacg gctcaagata gaggaaagca
     3421 aggacaagac actgaccggc aaaaagccca agcgtttcgc gcctggcggc cagagcacac
     3481 agatgattgt cggcgcggat gccaccgatg accgtgccat cctggatacc agcgctaacc
     3541 tgtatggcgc ttaccgtttg cggcgtgtct attactctgc ctttagcccc atccccgatg
     3601 ccagcaagat gttgccgctc aaggcaccac ccttggtacg cgaacaccgg ctctaccagg
     3661 ctgattggct gctgcgcttt tacggttttg aggccgacga gatcgtgacc gggcacgatg
     3721 gcatgctgga cctggatgtc gatccgaaga cggcctgggc gctgcaacat cgcgaacagt
     3781 ttccagtaga ccttaaccgt gcatccaaag ccatgttatt gcgtgtccct ggattggggg
     3841 tcaccagtat tgcccgcatc ctggcggcgc gcaaagtacg tgccatacgc tacgatgacc
     3901 tggcccgctt gcgggtttcg cttaaaaagg tgctgccgtt tattctgctg ccggattatc
     3961 atccaggaca tcgtcttgac gaccaacact taaaacagcg tttgcagcgg accatcccac
     4021 cacaacagtt tggcttattt gagtccgtct agtatggtgc tggtccggct caaaaatgaa
     4081 actgattttg acggttggcg tactgcagca cggtccttgc tggcgcaagg cattgcgcct
     4141 gaggcggtag actggcgggt gagtgataca agtcctgggc tatttgatac gcctgagttt
     4201 gtccccgcgc atatacctgg ctgggcattc agtgtctccc gtgatttctt tcagctatgc
     4261 aagaccgcta tcctttaccg cgatcccatg cgctttggcc tgctttaccg cttgctttgg
     4321 cggctgcatg aagtgcccga gctgttacag atgtcgtttg atccggatgt gattaaagca
     4381 agacagatgg cacaggccgt ccggcgtgac ctgcacaaga tgaaagcatt tgtccgtttc
     4441 cgcgaaatca agatgcaaga ccaggaacca catttcgtcg cctggtttga gcccagccat
     4501 catattgtag aagcatccgc agcctttttc agcggccggt ttaccaatat gcgctggtcc
     4561 atcctcaccc cggaaatctg tatgcattgg gatagccatc agttgcatta cagcccgggg
     4621 gcgagtaagg cagatgcacc ggccgaggac gcggacgaag tgttatggca gtcttattac
     4681 cgtagtattt tcaatccggc acgcctcaag gtatcggcaa tgctgtcaga gatgccgaaa
     4741 aaatactggc gcaacctgcc cgaagcagcc ctcattgcag acctgatcgc caacgccgat
     4801 cagcgcaagg atatgatgat tgcggcagcg gcgacagaac cgacacgcaa gatcgtgaaa
     4861 tatctccccg acaaggaagg ggacgggatg agggcggaag ccgtaggcgc acaggctgcc
     4921 ttataccggc tcaatgagga gatgttagcc catggggcgt ttgaactcgc tgcctatgct
     4981 actcaggcgg tgatgggcag cggtgccgtg ccatcgtcta tcctattgct gggcgatcag
     5041 cccggggagc aagatgatct cgctggccag ccccttaccg gtccggttgg cacattgttg
     5101 gataaagcct tgaatgcgag gggagtaggg cggtcaaatg tttacgtgac caatgtactt
     5161 aaacattacg ctttcaggtt acagggtacg cgacgcgtag cacgtgcgcc agaagcgcgt
     5221 gaaatccagg cctatttgcc ctggttgcgc gccgaaatcg caattgtaga accctcagtg
     5281 atcgtcgctc tcggtccggt tgcggcacgt gcgcttactg gcagggcgat ggatatggaa
     5341 gcctatcggg gaaaacggtt atcgctgacg gatggccgtc aggtagtagt gacatatcac
     5401 cctgccatga tcttgcgcat agcggatcag gcaacaaaac agttgcgtta cgcacagctg
     5461 ctcaatgacc tcaccttggc ggcaaaggtg gcgacgcagt gtgtgtttga aaaaagttaa
     5521 aaggtgatga ggcggaaacg tttacgccgc catcgcgcag cgatgcgcat atagctgcgt
     5581 aagctcctca cccaacagct tacatgcctc ctcgtaagtt acgcgggaaa gcaatatgcg
     5641 gtcacctttg gcaggcacaa acaaaaagga ttgattactc acatcagtaa taaactcacc
     5701 gacaatggcg tcattaatgg tgacaacatc caggttggcc tgcagttctg attttttgaa
     5761 ttctattttc atcattattg ccttatgtag tcactcacat aaagagaggc ttccgcagaa
     5821 gccctctcgt ctccagcacg acccgccctg acaaggggtt gccttgcgtg ctgtatatat
     5881 gaagattaac ggcaaaaaat ggataaattt atagggcaaa tgcgcgttat tttgtaggaa
     5941 tatctttacg tctctggaga ggtgctttcc atttc
//
LOCUS       DATMSK010000217         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_369090,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000217 DATMSK010000000
VERSION     DATMSK010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369090"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VNZ84_07285"
     CDS             complement(<1..1145)
                     /locus_tag="VNZ84_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWU66797.1"
                     /translation="MHNWLLPEYIEDVLPAEAARLETYRRQLLDLFRVHGYQYIVPPM
                     MEYVESLLTGTGHDLDIATFKVVDQLTGRLMGLRADTTPQAARIDAHMLNHQGVSRLC
                     YAGTVLRTKPDGLARTREPLQLGAELYGHAGIESDIEVQRLMVKALQLLGMRTLFVDL
                     SHAAVFAGLSHMAQLGVQQEQSLQAALQAKDITLVETLVANLPEKSRNAFIALTQLNG
                     GIEVLEQASDTLPASAELEQALYELETVAKALNNADVTVTIDLSELRGYHYHSGMVFA
                     AYAKGYAGPIALGGRYDEVGAAFGRARPATGFSLDLRGALQALGPATLNKGILAPVAA
                     DAELLSLIDTLRSQGQVVVEALPDMPVNYSELACDRTLQKVDGHWQVV"
     gene            complement(1268..1447)
                     /locus_tag="VNZ84_07290"
     CDS             complement(1268..1447)
                     /locus_tag="VNZ84_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HWU66798.1"
                     /translation="MNWWWLLGGVLLIEGLMPLLFTQAWRQTFEKLLQLRDGQIRFIG
                     FASALIGLLILWFNH"
     gene            complement(1462..2331)
                     /gene="hflC"
                     /locus_tag="VNZ84_07295"
     CDS             complement(1462..2331)
                     /gene="hflC"
                     /locus_tag="VNZ84_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HWU66799.1"
                     /translation="MRNIGSLLIGVFVLLVIITSSAYTVDQREYALVFRLGEIVSVKS
                     EPGLYFKMPMVENIRYYDKRILTLNWEEPDRFITSEKKNVLVDSFVKWRIVDPSKYYV
                     SVRGDEVQAERRLSQMVNDGLRAEFGKRTIHDVVSGERSEIMEILRQRADKESRQMGI
                     QILDVRLRRVDLPKEVSESVYQRMEAERKSVANELRSQGAAAAEKIRADADRQREVII
                     AEAYRDAQKVKGEGDAKAAEVYAQAFGKNPEFYAFYRSQEAYKNSFKSKSDVMVIDQS
                     SDFFKYMRSSGKK"
     gene            complement(2354..3547)
                     /gene="hflK"
                     /locus_tag="VNZ84_07300"
     CDS             complement(2354..3547)
                     /gene="hflK"
                     /locus_tag="VNZ84_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HWU66800.1"
                     /translation="MRKFPGFWNKNNEGPPDLDEVFKDLNQKLNRLFGNKGGQGGGPR
                     SAAPAPQPLPVAPILGLVALIWLGSGFYIVDQGSRGVVLRFGKHVETTMPGPRWHLPF
                     PIETVENVNLEQVRTIEMGYRSFDSESGRSKELRESLMLTDDENIIDLQVAVQYNLKS
                     VEDYQFSNRSTEESVRGVAETAIREVVGKSKMDFVLYEGREEVAAKAKKLMQEVLDRY
                     KSGINIVNVTMQNAQPPEQVQAAFDDAVKAKQDLERQKNEGQAYANDVIPKARGTAAR
                     LAEEAAGYKLRVENEAKGNADRFEQILAQYNKAPEVTRQRLYLDAQEHILANTSKVLV
                     DQKGGNSLLYLPLDKLMTMSGAGASGSSASAAASEKPVQEPQADSSVLDRSRDAMRNR
                     DREYR"
     gene            complement(3611..4738)
                     /gene="hflX"
                     /locus_tag="VNZ84_07305"
     CDS             complement(3611..4738)
                     /gene="hflX"
                     /locus_tag="VNZ84_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWU66801.1"
                     /translation="MFDRPTGGDAVVLVSVNFGDADYEESLQELRHLAGTAGLNISAT
                     LEGKRQTPDPKYFIGSGKADELKALMAAQESKVAAFNHDLSPSQQRNLEKLLEARVVD
                     RTGLILDIFAQRAQSHEGKLQVELAQLEHLSTRLVRGWTHLERQKGGIGVRGGPGETQ
                     LELDRRMLRVRVKQLREKLGKLKQQRGMQRRARKRSNLMTVSLVGYTNAGKSTLFNRL
                     THSGVYAADQLFATLDTTSRKMHLPEGYPVVLSDTVGFIKHLPTTLIEAFGATLEEAA
                     QADLLLHIVDVASPNRDAQIEQVNIVLNEIGAAQVPQILVLNQIDRMGMAPGIDRDEY
                     GNIRTVRVSAKDGTGIEDLRQALLEHQQYLKKLSTEESAYV"
     gene            complement(4742..>4933)
                     /gene="hfq"
                     /locus_tag="VNZ84_07310"
     CDS             complement(4742..>4933)
                     /gene="hfq"
                     /locus_tag="VNZ84_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWU66802.1"
                     /translation="KEHVQVSIYLVNGIKLQGQVDSFDQYVILLKNTVTQMVYKHAIS
                     TIVPARPVSLAPHETAQES"
BASE COUNT         1224 a         1374 c         1093 g         1242 t
ORIGIN      
        1 accacttgcc agtgcccatc cactttttgc aaagtacggt cacaagccaa ttcggaataa
       61 ttcacaggca tatcaggtaa cgcttccacc acgacttgtc cttgtgaacg cagggtgtca
      121 atcagcgaca ataattcagc atctgcagcc acgggggcaa gaatgccttt gtttaatgtg
      181 gcgggaccca aagcttgcaa tgctccgcgc aaatcaaggc taaagccggt ggcaggacgt
      241 gcacggccaa atgcggcacc gacttcatca taacggccac ctaatgcaat cggcccggca
      301 tagcctttgg cgtaggcggc aaataccatt ccgctatggt agtgatagcc gcgtaactcg
      361 ctcaaatcta tggtaacggt aacatctgca ttgtttaaag ctttagccac tgtttccagt
      421 tcgtacaatg cctgctccag ctctgcagag gcaggcaatg tatcacttgc ctgctccagc
      481 acctctatgc caccgtttaa ctgagttagc gcaataaagg cattgcggga tttttcaggc
      541 aggttggcta ccagcgtttc aaccagtgtg atgtcttttg cctgtagtgc agcctgtaga
      601 gattgctcct gctgtacgcc cagttgcgcc atgtgtgata acccagcaaa aacggcagcg
      661 tgactcaggt ccacaaacaa agtgcgcatc cccagcaact gtagcgcttt taccatcagt
      721 cgctgcacct caatatcact ttcgataccg gcatgcccgt acagctcggc accgagttgt
      781 aaaggctcac gtgtgcgcgc caagccatcc ggcttggtac gtaatacggt acctgcataa
      841 cacaatctgg aaacaccctg atgattgagc atgtgcgcat caatacgcgc ggcttgcggc
      901 gtcgtatcgg cacgcaaacc catcaaacgg ccagtcagtt gatctacaac cttgaatgtc
      961 gcgatgtcca ggtcatgtcc tgtacccgtt aataacgact ccacatattc catcatgggt
     1021 ggtacgatgt attgatagcc atgaacccta aacaagtcca gcaactgtct gcggtaagtc
     1081 tcaagacgcg ctgcttccgc aggcaataca tcttcaatat attcaggaag taaccaatta
     1141 tgcatcccgc cattatacct gcaaaccccc acctaagaca cgggtttagc aatcgcgatt
     1201 gacaacgctc gtggcaacgc acaataaaaa agcacaccca aggtgtgctt tttagatgtc
     1261 catacgatta atggttaaac cacagaatca acaggcctat cagagcagaa gcaaatccga
     1321 taaaacggat ctgcccatcg cgcagttgca atagcttttc aaaggtctgg cgccaggctt
     1381 gtgtaaacag taaaggcatc aacccctcta tcagcaacac accacctagc agccaccacc
     1441 agttcatcgc aagcgtttaa attatttctt gccgctactg cgcatgtact tgaagaaatc
     1501 tgagctctgg tcaatcacca tgacgtcaga cttactcttg aagctgtttt tataagcttc
     1561 ctggctacgg taaaaagcat agaactctgg atttttacca aatgcctgcg catatacttc
     1621 agccgctttg gcatcgccct cacctttgac tttttgtgcg tcgcggtagg cttcagcaat
     1681 aatcacttcg cgttgcctgt cagcatcggc acggattttt tcagccgctg cagcaccctg
     1741 tgaacgtaac tcattagcga cagacttacg ctcggcctcc atacgctgat acacggactc
     1801 acttacctct ttaggcaaat caacacggcg caaacgcaca tccagaatct ggatacccat
     1861 ctggcggctt tccttgtctg cgcgctgacg caggatttcc atgatttctg aacgctcgcc
     1921 agagacgacg tcatggatag tgcgtttacc aaactcagca cgcaagccat cattcaccat
     1981 ttgtgacaaa cggcgctcag cctgcacttc atcaccgcgg acagacacat agtatttgga
     2041 cggatcaaca atgcgccatt tgacaaatga atcgaccagt acgtttttct tttcactggt
     2101 aatgaagcgg tcaggctcct cccagttgag ggtcaggata cgtttatcgt aatagcggat
     2161 attctccacc ataggcatct tgaagtagag gccaggctca cttttaaccg acacaatttc
     2221 accgaggcgg aacaccaaag catactcacg ctgatccacg gtataggctg aagaggtaat
     2281 gatcaccagt agaacaaata ccccgattaa cagacttcca atatttctca tcataatatt
     2341 ccttcagggc agactagcga tattcgcgat cacgatttct catggcatca cgagagcgat
     2401 ccagtacgct tgaatcagct tgtggctctt gcaccggttt ttcggaagcg gctgcagatg
     2461 cagaggatcc actcgcgccc gcgccactca ttgtcatgag tttatccagc ggcaaataca
     2521 gcaaactgtt gccacccttc tggtcaacca gcactttgct ggtattggcc aatatatgct
     2581 cttgcgcgtc cagatacaga cgctgacggg tcacttcagg cgccttgttg tattgcgcca
     2641 aaatctgctc gaaacgatca gcattacctt tagcttcgtt ttccacacgc agtttgtaac
     2701 ctgcagcctc ttcggccaaa cgggcagctg taccacgcgc tttcgggatc acatcattcg
     2761 cataagcctg gccttcattt ttctggcgct ccagatcctg cttggctttg acggcatcat
     2821 caaatgccgc ctgcacctgt tcgggcggtt gggcattctg catggtgacg ttgacgatat
     2881 tgataccgga tttatatcga tccagcactt cctgcatcag ttttttagcc ttggcagcca
     2941 cttcttcacg accctcgtac aagacaaaat ccattttgct tttgccgacc acttcacgaa
     3001 tggctgtttc tgcaacacca cgcacgctct cttcagtaga acggttactg aactgatagt
     3061 cttctactga cttcaagtta tattgcaccg ccacttgcaa gtcgatgata ttttcatcat
     3121 ccgttagcat cagcgactcg cgtaactctt tactgcggcc agactcactg tcaaaagaac
     3181 ggtaacccat ttctatggta cgcacctgct ccaggttcac gttttctacc gtttcaatcg
     3241 ggaatggtaa atgccagcgc gggccaggca tcgtggtttc tacatgtttg ccgaaacgca
     3301 ataccacccc acgcgaaccc tggtcgacaa tataaaaacc ggagcctaac caaatcaaag
     3361 ccaccaaacc aagaataggc gcgacaggca atggttgtgg cgcaggcgca gcgctccttg
     3421 gaccaccacc ctgtccacct ttgttaccaa acagacggtt cagcttttga ttcaaatcct
     3481 tgaatacctc atccaggtcg ggagggcctt cgttattctt attccaaaag cctgggaatt
     3541 ttctcatgct agtactccgt attttttgat tttcagacac atactgaata gttacctact
     3601 cagtatcgcc ttagacataa gcactttctt ctgtactcag ttttttcaag tattgttgat
     3661 gctccaacag tgcctggcgc aagtcttcaa tgccagtacc gtctttggcc gagacgcgaa
     3721 cagtacggat attaccatac tcatctcggt cgattcccgg agccatgccc atgcggtcaa
     3781 tctggttcag caccaggatt tggggcactt gcgctgcacc gatttcattc aagacaatat
     3841 tgacctgttc gatttgtgcg tctcggttag ggctggcaac atcaacgatg tgcagcaaca
     3901 agtctgcctg cgcagcttct tccagcgtcg caccaaaagc ttcaatcaag gtagttggca
     3961 aatgcttgat aaacccgact gtatcggaca gcacgaccgg atagccctcc ggcagatgca
     4021 ttttgcgcga agtcgtatcg agggtagcaa acagctggtc ggctgcatac acgcctgagt
     4081 gcgtcagtct gttgaacaag gtagatttac cagcgttggt atacccgacc agtgacactg
     4141 tcatcaaatt tgaacgttta cgtgcacgcc gctgcatacc acgttgctgc ttgagcttac
     4201 ccagcttttc acgcaactgc ttgacgcgca cgcgcagcat acgcctgtcc aactccaact
     4261 gggtttcacc agggccacca cgcacaccaa tcccgccctt ttggcgttca aggtgggtcc
     4321 agcctcgtac caggcgagta gacaaatgct ccaactgcgc cagctctacc tgcaacttgc
     4381 cttcatgcga ttgcgcacgc tgggcaaaaa tatccaggat caagccggta cggtctacca
     4441 cccgagcttc aaggagtttt tccaggttac gttgctgtga cgggcttaaa tcatgattaa
     4501 aggcagccac tttactctct tgggccgcca tcaaagcctt aagctcatcc gccttacctg
     4561 agccgataaa atacttggga tcgggagttt ggcgcttgcc ttccagcgtc gcagaaatat
     4621 tcagtccggc agtgccggcg aggtggcgga gctcttgcaa actttcttca taatcagcat
     4681 caccaaaatt gacactgacc agaacaacgg catctccgcc ggttggtcta tcaaacatgc
     4741 attaagattc ttgtgcggtt tcgtgcggtg ccaaactcac tggacgggct gggacaatcg
     4801 ttgaaattgc gtgtttataa accatttgag tcacggtgtt tttcaacaga atgacatact
     4861 ggtcaaacga gtcgacctgg ccttgcagtt taatcccgtt taccaggtag atagacactt
     4921 gtacgtgttc ttt
//
LOCUS       DATMSK010000218        13703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_111169,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000218 DATMSK010000000
VERSION     DATMSK010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 13703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13703
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111169"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VNZ84_07315"
     CDS             <1..344
                     /locus_tag="VNZ84_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU66803.1"
                     /translation="TIVFDRRHINQILWNLCKNGWRHSQQHAQSLQLKIAGDKSGQFI
                     HITIKDDGSGIDPNIQPHLFEPFMTTEKTGTGLGLFIARELAEANGAKLNYTSSSNGT
                     QFSLTVKKASV"
     gene            345..1847
                     /locus_tag="VNZ84_07320"
     CDS             345..1847
                     /locus_tag="VNZ84_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWU66804.1"
                     /translation="MANSYRCLVVDDETDIRELVVLTLERMGIQAESASSVTDAKHML
                     HSFSYDLCLTDMRLPDGLGLELVQHINAQFPGLPVAVITAYGSAENAVSALKAGAYDY
                     ITKPISLKQLRPLVESALKLSSQKESHGANTVDLIGASPVMSYVRTMIAKLARSQAPV
                     YISGESGSGKELAARLIHLNSSRKDQSFIAVNCGAIPENLMESEFFGYKKGAFTGAIQ
                     DTQGLFQAANGGTLFLDEVADLPLAMQVKLLRAIQEKKVRVVGSTSEEAVDVRIISAT
                     HKNLNAMMEKGEFRQDLYYRLNVIQLKMPSLRERPEDIPELTERLLGKLCQSQGIGIP
                     SISDEAKVYIQQLPFHGNVRELENMLERALALCDGLSITVEDLSLDEIPVNLNDKPVL
                     PWNAPVTEPSVMSSTIPVPAFDDNRPVPSPANPSAGSTMLPMDISLSDYLEDIEKRTI
                     LQALEKTNNNKTAAAKLLGISFRTLRYRLSKLGLSKEQDPDLEEAEGDEE"
     gene            complement(1900..2910)
                     /locus_tag="VNZ84_07325"
     CDS             complement(1900..2910)
                     /locus_tag="VNZ84_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HWU66805.1"
                     /translation="MVFFQLLLMLLLILVAAEVFTNALEHLGERLGISEGVTGSIFAA
                     VGTALPETLVPLLAIFSYTSSTGDSHAGHDIGVGAILGAPLMLATLSISLMALSVLKR
                     RGSKGHIRPERTGLIRDLNFFILAFVFATIAMFIPHTEALVRYGISILMILIYFVYVL
                     MTIKASKGLVEEGHATEAEDVMMLSRLGLPTNMITIILQLLIGLGLLIAGAKGFINEV
                     ETAAAILGVSALLLSLLIIPIATELPEKVNSILWIRKGKDTLAFGNITGAMVFQGTLL
                     PAIGIMLTDWSPRQEVAWGILITLLAAIWVRHRIAKGGLLVWHLLVNGAFYLAYLAIV
                     LG"
     gene            3037..3588
                     /gene="ampD"
                     /locus_tag="VNZ84_07330"
     CDS             3037..3588
                     /gene="ampD"
                     /locus_tag="VNZ84_07330"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HWU66806.1"
                     /translation="MSLTSVPTIEIDASGLANTAKWILSPNCDARPFSTEVDMIVIHN
                     ISLPPHQYGGQGIMQLFTNQLDPQEHPYYAEIAHLKVSSHFLIRRTGELIQFVSCEQR
                     AWHAGQSHWQGRERCNDFSVGIELEGSDIEVFEDIQYITLQMLITALQKRYPIKHIVG
                     HSDIAPGRKTDPGPFFDWQRLFD"
     gene            complement(3585..4502)
                     /locus_tag="VNZ84_07335"
     CDS             complement(3585..4502)
                     /locus_tag="VNZ84_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWU66807.1"
                     /translation="MKESTHPYYGAMLVLLSSIAFSSKAIMVKLAYVYHIDAATLIAL
                     RMAFAIPFFAGLGWWAMRQGMPVSMTGKDWTTLVLLAVVGGYGSMWLNFEGLRYVSAG
                     LERVILFLYPTLVVAMSAIFLKHQITRREWFAMVTSYAGVVMVVWHDVELAGLASTET
                     LYGAWLVLLSAVVYAAYLLVSGQLIPKLGASRFTALTMSLAAFASACHFGASESLSGV
                     MGLPHGVYALAFLMALIATVLPSVLMNMGIHQLGSRKASLISAIGPVSTIVLAYLFLG
                     ESLSWIQAIGTALVLAGVMVVSLDNTAKR"
     gene            complement(4728..5255)
                     /locus_tag="VNZ84_07340"
     CDS             complement(4728..5255)
                     /locus_tag="VNZ84_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU66808.1"
                     /translation="MLEQRLALVSDIPVLCALVNAAYRGETSRAGWTTEADLLTGVRT
                     SERTIQALMAREDACILIGEEAGGVMAASICLERHPAQEDTVQFGMIAVSPLLQNRGY
                     GKTMILAAEKLAMSQWGAQRAQMMVISLREELIAFYQRLGYQPTGEILPFPHQPDMWQ
                     PQVENMQLITFQKTL"
     gene            complement(5268..6497)
                     /locus_tag="VNZ84_07345"
     CDS             complement(5268..6497)
                     /locus_tag="VNZ84_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HWU66809.1"
                     /translation="MNKLGLNGQILLAVMTAVALGVFAQGKDVESALYAANLVGTLFI
                     DLLKMVMIPLVFCAITTGIANLRKHSQIDIVWKTTLLFFATTTLIAVAVGLGASHLFK
                     PGAGLHLDMFANAMQQFESKQMTLPEFFAQFLHGIFMNPFKALADGNILPIVSFALVL
                     GIALVTASERYPQLLGLFEEGMEVSMQLINWIMRLAPFGIAALLFKLVATQDLSLFAS
                     LTKFILVVTGTTLFHGLIVLPALLFLVTGKRPWWFLRRAKESLLTAFATSSSNATMPV
                     TMRCATQNMQVKPEIAGFVVPLGATVNMDGTALYEAAAALFVAQLAGIELSLGQQLVI
                     CFTTMIAAMGAPGIPSAGMVTMVMVLQSVGLPAEAIAILLPIDRLLDTVRTTVNVEGD
                     MIGSLVVQHVTDTRLGR"
     gene            complement(6584..7861)
                     /locus_tag="VNZ84_07350"
     CDS             complement(6584..7861)
                     /locus_tag="VNZ84_07350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HWU66810.1"
                     /translation="MNQTLMRQTAMLLNDMLKFESPADAKLGEFFRQHRELGTKDRAW
                     VAETAYGVLRRYRYLREVSMSESDEQEDTRKLIIAWMLKVEGKSIRDLDDMLDERQKE
                     WAVAIKAKNSEGFAPAVLADVRDWFWNKLVAQYGETEAMTICRSMFEQAGLDLRVNTI
                     KSNREEVLSRMLAENTVKDNVITPTPYSPVGIRMAARLNIGKHVLFTEGKIEVQDEGS
                     QLLSYLVAPKRGQMVADLCAGAGGKTLALGALMKNTGRLYAFDVSEKRLNNLGQRLKR
                     SGLSNLQAQLINNENDLKLKRLNGKFDRVLVDAPCSGMGTLRRNPDLKWRQAEEDVAE
                     LNVKQTNILARAAKLCKAGGRVVYATCSLLRDENEAIAEAFLKAHPDFKLVPANEVLA
                     QQNIVLDTGEYLKLLPHLHGTDGFFAAVFEKAA"
     gene            complement(7883..8974)
                     /locus_tag="VNZ84_07355"
     CDS             complement(7883..8974)
                     /locus_tag="VNZ84_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWU66811.1"
                     /translation="MQSSGLQHWMRQHVSWPLALALCLSLLLHVFVFSEVDWRDWFLS
                     EKHEPEVLQARLLPPPQPLAKSLPSPVKPKPVKTQPVQAPQPVVTSNVEPPVEAVPVD
                     DAASEVQAVEQQPQPPALSEEEQQARLAEIAAWHEMDHPDVVPDESQPAPYQHVSTDF
                     SIFVNGEERPAGNANIEYLRDDSGHYSLRWLVEGKGLLKLLYPSLEQQSQGEISPHGL
                     KPHFYRYAFGSRASKTYEATFDWEINIITLKTSKGEQRLDLRVNTQDLLSFMYQFMFV
                     PPLQEMHVTLTNGRRLGEYEYMFEGEEPLVIADQTIQTVHIAHTRGDTDEKVDLWLAS
                     DYRYVPVKIKKLEKNGMVIEQIATRLIAE"
     gene            complement(8997..9680)
                     /locus_tag="VNZ84_07360"
     CDS             complement(8997..9680)
                     /locus_tag="VNZ84_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWU66812.1"
                     /translation="MGRLIASVLLAILWLASPVLSAAPKQLTLKYDLLQQGISIGSVQ
                     DQLSVTGNRYQIQSLAEGKGIYKLMGERTLSSQGRIISNALRPDRFESHQSKRPNKAL
                     ISEFDWAKKVLKMQIKDEQETDTLTKGTQDLLSVMYCWMWQPPKGKQVNLAVANGKKL
                     SPHAFVVTEESAPLETEAGTFNVVKLTDAEGEKTLYLAKDKNYLPVKLVVEDDGKHME
                     QVITSITGQ"
     gene            complement(9704..10753)
                     /gene="purM"
                     /locus_tag="VNZ84_07365"
     CDS             complement(9704..10753)
                     /gene="purM"
                     /locus_tag="VNZ84_07365"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWU66813.1"
                     /translation="MLTTSSDKTSITYRDAGVDIEAGDALVEQIKPFAKRTLRPEVMG
                     GIGGFGSLFAMPKKFKEPVLVSGTDGVGTKLKLAFQLNKHDTVGIDLVAMSVNDILVQ
                     GAEPLFFLDYFACGKLEVGTAAAVIKGIAQGCEESGCALVGGETAEMPGMYPAGEYDL
                     AGFAVGCVDKANIIDGSTIKAGDVVLGLASSGAHSNGYSLIRKLIDKSGIDMESDFHG
                     RSFKDVVMAPTKLYVKSILKLLETLPVKGMAHITGGGITENIPRVLPEGLTAEVQKSS
                     WTLPPLFQWLQVQGNIEPLEMYRTFNCGIGMAVIVAAEHAEQAQAVLAGAGETVYRIG
                     QIRAQQAGEAPTVVV"
     gene            10927..12057
                     /locus_tag="VNZ84_07370"
     CDS             10927..12057
                     /locus_tag="VNZ84_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWU66814.1"
                     /translation="MSDQEHQKSIATQFVSDHRWWIVTAVVIYLFYLLEPILTPFLAA
                     AVIAYMLDPLVDKLSEVGYRNRQIGRTLATLLVMSGVILVMIGLLLIIIPLLQQQSTL
                     IAQRLPLLLDHFHQQVEPWLLQRFGIHLNIDRTDIQKLISEHWQTAGSMIGNVLLSAG
                     QKGLNLIGMIANILLLPVVLFYFLRDWDDMMAEIGDLIPRNWIGRIKALCKDIDSVVA
                     EFLRGQLSVMLSLCVFYSIGLWLVGLDMALSIGMIAGLLSFVPYLGFALAFILAVLLA
                     LLQFASLPEVIPVLLVFGIGQFVESFFLTPILVGDRIGLHPVLVILALMAGGQLFGFA
                     GVLLALPVSAAIAVGVRHTKQSYLRSEAYLNSPPHIIQTLDE"
     gene            12084..12683
                     /locus_tag="VNZ84_07375"
     CDS             12084..12683
                     /locus_tag="VNZ84_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HWU66815.1"
                     /translation="MKQLLLNIQPPAAPGFDNFIVGQNQEALASVRSVLADAATTRFV
                     YLWGESGSGKSHLLLAAQAMGAHIADDVHTLDEESQIILFDTYNQIKAEGGVLITAGS
                     HAPTQMGLRADLATRLAWGLVYQLLPLSDDEKAEALRAHARDRGMKLPDEVIAYCLRY
                     LRRDLPTLMAVLEALDEWSLTTKKAVTVQSLKKMLQATL"
     gene            complement(12697..>13703)
                     /locus_tag="VNZ84_07380"
     CDS             complement(12697..>13703)
                     /locus_tag="VNZ84_07380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWU66816.1"
                     /translation="GKAITDAENGQRGYLLTGHMLFLDSLEQGRAETEKAISRLERQS
                     KNFQEVKPTLEKIKRLVHQKFEVMDQSIQVQLHAGEYASHLSLSKDKSRELMKLINDQ
                     LATIDMKLEHLRVNFEQEIRQRMFESLLGAILLGILIIGVLWFSYRSTTHLLEQVIEN
                     QAVAKQASHQAGHDLLTGLPNRRSVDVHLASTYQMARHASQQFAILFMDLDGFKLVND
                     KYGHEVGDALLIEVASMFKKVLRQSDFLARQGGDEFVLVVGNYLYRLELTQLAHRLIQ
                     LFAQPVIVNGIPLEIGVSIGIAEYPHHGKSVKQLLHVADKAMYDSKKKGKNRYSFGPE
                     "
BASE COUNT         3480 a         3779 c         3205 g         3239 t
ORIGIN      
        1 gtactatcgt ttttgaccgc aggcatatca accaaatcct atggaattta tgcaaaaatg
       61 gatggcggca tagtcagcag catgcacaaa gcctgcaact taaaatcgcc ggggataaaa
      121 gtggccaatt tatccacatc accatcaagg atgatgggag cggcatagac cccaatatcc
      181 agccgcactt attcgagccg ttcatgacaa cggaaaaaac tggcacgggt ttggggttgt
      241 ttattgcacg agagcttgca gaagccaatg gtgcaaaatt aaactacacc agcagctcaa
      301 atggcactca attttcactg acagttaaaa aggcaagcgt ttaaatggca aattcctatc
      361 gttgtctggt ggtcgatgac gagaccgata ttcgagaact ggtggtattg acactggaac
      421 gtatgggcat acaggcggaa agtgccagct cggtcaccga tgccaagcac atgttgcact
      481 catttagtta cgacctgtgc ctcacggaca tgcgcctgcc agatgggctg ggactggaac
      541 tggtacagca cattaatgca caatttcctg gattaccggt cgccgtcatt accgcctacg
      601 gcagtgccga gaatgcagtg tccgctctca aagctggcgc ttatgactac atcaccaagc
      661 cgatctcact caaacaatta cgtcccttag tagagtccgc actcaagctg tcgtcacaaa
      721 aagaatcaca cggggctaat acggtagacc tgattggtgc ctccccagtc atgagctatg
      781 tacgcaccat gatagccaaa ctggcacgca gccaggcccc cgtttatatt agcggcgaat
      841 ctggcagtgg taaggagttg gctgccaggc tcattcacct caacagctcg cgcaaagacc
      901 agtcgtttat tgccgtcaac tgtggtgcca tcccggaaaa cctgatggaa agtgagttct
      961 tcggctataa aaaaggcgca tttacgggcg cgattcaaga tacccaaggc ttgttccagg
     1021 cagccaacgg tggcaccctg ttccttgatg aagtagctga tcttccactg gcaatgcagg
     1081 tgaagctgct gcgtgcaatt caggagaaaa aagtgcgcgt agtcggcagc accagtgagg
     1141 aagccgtaga tgtgcgcatc atttctgcca cccacaaaaa cctcaatgcc atgatggaaa
     1201 aaggcgagtt caggcaagac ttgtactacc gcctcaatgt gatccagctt aaaatgccat
     1261 cattgcgcga gcggccggag gacattcccg aactgaccga gcgcttgctg ggcaaactct
     1321 gccagtcaca gggcatcggc atccccagca tttccgatga agccaaggtc tacatccagc
     1381 aactaccttt ccatggcaat gtacgtgaac tggaaaatat gctggagcgt gcattagccc
     1441 tctgtgacgg ccttagcatc acggttgaag atttgtcttt agatgaaata cccgtgaacc
     1501 tgaatgacaa acccgtatta ccatggaacg cccctgtcac tgagccatcg gtcatgtcca
     1561 gcaccattcc agtacctgcc tttgatgaca atcggcctgt gccaagccca gccaatccat
     1621 ctgccggcag taccatgcta ccaatggaca tcagcctatc cgattatctg gaagacattg
     1681 agaaacgcac catcttgcag gcccttgaaa aaacaaacaa caacaagaca gccgccgcca
     1741 aactattagg gattagcttc cgtactttac gctatcgatt atccaaactg ggactctcca
     1801 aagaacagga tccagatctt gaggaagccg aaggcgacga agaataaaaa agggcgactg
     1861 tatggtcacc cttttcacta ttgagcttaa cttggcagct tagccaagca caatggccag
     1921 ataggccagg tagaatgcac cattgaccag caaatgccag accagcaggc cacctttggc
     1981 gatacggtgg cgtacccaga tcgccgccag caaggtgatc aatatgcccc aagccacttc
     2041 ctgcctgggc gaccagtccg tcagcataat accgatggct ggcagcaagg tgccctggaa
     2101 taccatggca ccggtaatat taccaaatgc cagagtatcc ttgcctttac gtatccacaa
     2161 aatgctgttt accttttcag gcaactcggt agcgatgggg ataattagca aagacagcag
     2221 caaagcagaa acacctaaaa tagctgcggc ggtttccacc tcgttaataa agcctttggc
     2281 ccctgcaatc agcaacccaa gaccaatcag caactgcaga ataatggtga tcatgttggt
     2341 tggcaatccc agtctggaca acatcatgac atcctcagcc tcggttgcat gtccttcctc
     2401 taccagccct ttggaagctt taattgtcat caacacatac acaaaataga tcaggatcat
     2461 gaggatactg ataccgtagc ggaccagtgc ctcggtatgc gggataaaca tggcgatggt
     2521 ggcaaacaca aaggccagga taaagaagtt gaggtcacgg atgaggccgg tacgttcagg
     2581 gcggatatgc ccttttgatc cacggcgctt caatacggag agcgccatta aactgataga
     2641 caaggtggcc agcatcaacg gtgcacccaa aatggcaccc acaccaatat catgcccggc
     2701 atgactatca ccagtcgagg aggtgtagga aaaaatagcc agcaacggca ccagggtttc
     2761 tggcagagcc gtacccacgg ccgcaaaaat agagccggtc accccctcgg aaatgcccag
     2821 ccgttcaccc aaatgctcaa gcgcattggt gaatacttca gccgccacca aaatcagtag
     2881 cagcattaac aataattgga aaaaaaccat gactgttcct gtctggcggc aatcaaccct
     2941 gcatgcgctc accgcctggc gcgcatgtgc atgacaaatg cgtacatgcg ggcggaatca
     3001 taacgcaatt cgcccaattt cacctacaat gtttgcatgt cactgacttc tgttccaacg
     3061 atcgaaattg atgcttctgg cttggccaat acggcaaagt ggatactgtc gcccaattgc
     3121 gatgcacggc cattttctac cgaagtggat atgatcgtga tccacaatat cagcctgccg
     3181 cctcaccaat acggcggcca aggcattatg cagttattca ccaaccagtt ggatccgcaa
     3241 gaacatcctt attacgctga gattgcacac ctgaaggtct cctcccattt tttgattcgc
     3301 cgtactggcg agctcataca atttgtcagt tgtgaacaac gtgcgtggca tgcaggccag
     3361 tcacattggc aaggtcgaga acgctgtaat gacttttcag tcggtataga acttgaaggc
     3421 agtgacattg aagtctttga agatattcaa tatattactt tgcagatgct catcacggcg
     3481 ctccagaagc gctaccccat caaacacatt gtcggccact ctgacattgc gccggggcgc
     3541 aaaacagacc ccggcccatt ttttgactgg caacgcctat ttgattagcg tttggcggtg
     3601 ttatccaggc tcaccaccat gacgcctgcc agtaccaatg ccgtcccaat cgcttgaatc
     3661 caggaaagac tctctccaag aaacaggtag gcgaggacga tggtcgacac cgggcctatc
     3721 gcgctaatca acgacgcttt acggctaccg agctggtgta tccccatatt catcagtacc
     3781 gaaggtaata cggtagcgat caatgccatc aaaaaagcca aggcatacac cccatgaggc
     3841 aagcccatga ctccgctgag cgattcactc gcaccgaaat ggcacgcgct ggcgaatgcc
     3901 gccaagctca tggtgagcgc agtaaaccgg ctggcaccta atttgggaat caattggcca
     3961 ctgaccagca ggtaagctgc gtacacaact gcgctcagca ataccaacca tgcgccatac
     4021 aaagtttcgg tggatgccag accagcaagt tcaacgtcat gccataccac catcaccaca
     4081 ccggcataac tggtgaccat ggcaaaccac tcacgacggg taatctggtg cttcaggaat
     4141 atggcactca tggcgaccac cagcgttggg taaagaaata agatgactcg ttccaacccg
     4201 gcagaaacat aacgtagccc ttcaaagttg agccacattg aaccataacc accgacgacg
     4261 gcaagcagca cgagtgtcgt ccagtcctta cctgtcatgg acacaggcat cccctgtcgc
     4321 attgcccacc agcccaatcc ggcaaaaaat ggaatggcaa atgccatacg tagcgcaatc
     4381 aaggtggctg catcaatatg gtaaacgtaa gcgagcttga ccatgatcgc ttttgacgaa
     4441 aaggcgatgg acgacagcag caccagcatg gcgccgtaat acgggtgggt agattctttc
     4501 ataggagccc tgaatcaatg gggctcgccg gggaatgaat aacaagatca accgcggaga
     4561 gcccgcggtt gcaaaaaact acgacaagca gcaaccgcgg cacgatgtgg cgaccagcca
     4621 caatgccttt cggttcatca tcacaacagt agattgattg aattgcttgt tcataagtaa
     4681 tgaaatgtac cacccgcttc aaggaggggt caagcgacaa gagacattca taaggttttt
     4741 tgaaatgtga ttaactgcat attctccacc tgcggttgcc acatatctgg ctggtgtggg
     4801 aaaggcagta tctccccggt cggctggtaa cctaagcgct gataaaatgc gattaactcc
     4861 tcgcgcaaac taatcaccat catttgcgcc ctttgcgcac cccattgcga catagccagc
     4921 ttttcagcag cgagtatcat ggttttaccg tagccacggt tttgcaatag cggtgaaacg
     4981 gcaatcatgc caaactggac ggtatcttcc tgcgcaggat gccgctcaag acaaatactg
     5041 gcagccatca cgccaccggc ctcttcacca atcaagatac aggcatcctc gcgcgccatc
     5101 agcgcctgta tcgtacgttc actggtacgc acaccggtca gcagatcagc ttcagtggtc
     5161 caaccggcgc gactggtctc gcccctataa gccgcattaa ccaatgcgca taaaacaggg
     5221 atatctgaaa ccagtgcaag gcgttgttct agcataatgg cgatacttta acggccgaga
     5281 cgggtatctg tcacgtgctg caccaccagg ctgcctatca tgtcaccttc tacattgaca
     5341 gtagtacgga ccgtatccag caaacggtct atcggcaata aaattgcaat ggcttctgca
     5401 ggcaagccta cactttgcag caccatcacc atggtcacca tgcccgcact gggaatcccc
     5461 ggcgccccca tggcggcaat catcgtcgtg aaacaaatca ccagttgctg gcctagcgat
     5521 aactcaattc cagccagttg tgcgacaaat aacgctgcgg cggcttcata caaagcggta
     5581 ccatccatat tgaccgtggc acccagcggc acaacaaacc ctgcaatttc cggtttgacc
     5641 tgcatatttt gcgtggcaca acgcatggtc actggcatgg tggcgttact tgagctggta
     5701 gcaaacgcgg tcagcaatga ctcttttgcc cgccgcaaaa accaccaggg gcgtttaccg
     5761 gtgaccagaa ataacaaggc aggcaagaca atcaggccat gaaacagggt ggtgcctgtt
     5821 accaccaata taaacttggt caatgaagca aacaaactca aatcttgcgt tgccactagc
     5881 ttgaacaaca gtgcagcaat cccgaaaggt gccaggcgca tgatccagtt gattaattgc
     5941 atggaaacct ccatgccctc ttcaaacaaa cccagcagtt gaggataacg ttcagaagcc
     6001 gtcaccagcg caatccccaa caccaaggca aaggaaacga taggcaggat attgccatcc
     6061 gccagcgctt tgaaagggtt cataaagatg ccatgcagga actgtgcgaa gaactctggc
     6121 aatgtcatct gtttggactc aaactgctgc atggcattgg caaacatatc cagatgtaaa
     6181 cctgccccgg gcttgaaaag atggctggca cccagcccga cagcgacggc aatcaatgtc
     6241 gtcgtggcaa aaaacaacag cgtggttttc cagacaatgt caatttggct gtgcttgcgg
     6301 agattggcaa tcccggtcgt gatggcacaa aacaccaacg gaatcatcac catttttagc
     6361 aagtcgataa acagggtacc gacgagattc gctgcataca aagcactctc aacgtccttg
     6421 ccttgcgcga aaacacccag ggccacggca gtcattaccg ccagaagaat ttgtccgttg
     6481 agtcctaact tattcatcat gtctatactc aggcaggcac caagataaga aaacccttca
     6541 tcctcatcaa aaatgaggca gtcaaagcgg cctcacacaa gacttaagca gctttctcga
     6601 acacggccgc aaagaaccca tccgtaccat gcaagtgtgg caataacttc aaatactcac
     6661 cagtatccag cacaatattt tgctgcgcga gtacttcatt cgccggtacc agtttaaagt
     6721 ctggatgcgc ttttagaaaa gcttcggcaa tggcttcatt ttcgtcacgc aacaaactac
     6781 aggtcgcata aaccacgcgg ccaccggctt tgcacagctt ggccgcgcgg gcgaggatat
     6841 tggtttgttt cacattcagt tcagcgacat cttcttctgc ctggcgccat ttcaaatccg
     6901 ggttacggcg caaggtgccc ataccgctac aaggagcgtc gaccagcact cggtcaaact
     6961 tgccgttgag acgcttgagt ttgaggtcgt tttcgttgtt gatcagttgc gcttgcagat
     7021 tggataaacc actacgcttc aaacgctgcc ccaggttgtt caggcgcttt tcggacacat
     7081 caaaggcgta taaacggcca gtatttttca taagggcacc cagtgccagt gttttacctc
     7141 ccgcccctgc gcacaggtcg gcgaccattt gcccgcgctt gggcgccacc agataactca
     7201 gcaattggct accctcatcc tgcacttcga ttttgccttc ggtgaacaac acatgtttgc
     7261 cgatgttcaa ccgggcggcc atgcggatac ctaccggaga atatggcgta ggtgtgatga
     7321 cgttatcttt aacggtattt tccgccagca tgcgtgaaag cacttcttcg cggtttgact
     7381 tgatggtgtt gacgcgcaaa tccagaccag cctgctcaaa catactgcgg caaatggtca
     7441 tggcttcggt ttcaccatac tgcgctacca gcttgttcca aaaccagtcg cggacatctg
     7501 ccagcaccgc tggggcaaag ccttcactgt ttttggcttt gatggcaacc gcccattctt
     7561 tttgacgctc atccagcatg tcatccaggt cgcgtatgct tttaccttcc accttgagca
     7621 tccaggcgat gatcagtttg cgcgtgtctt cctgctcatc ggattcactc atgctgactt
     7681 cccgcaggta acggtaacgg cgcaacacgc cgtaagcggt ctcagcaacc caggcacggt
     7741 ctttggtgcc taattctcgg tgctggcgga aaaattctcc aagcttggca tccgccgggc
     7801 tttcgaattt cagcatgtcg tttagcagca tcgccgtctg gcgcatcagg gtttggttca
     7861 tcaggttctt tcaatactta cattattcgg cgatcaaccg cgtggcaatt tgctcaatca
     7921 ccatgccgtt tttctccagc tttttgattt taaccggcac atagcgataa tcgctggcca
     7981 gccataagtc tactttctca tcggtgtcac cacgcgtatg cgcaatatgc accgtctgaa
     8041 tcgtttggtc ggcgatcacc agcggctctt cgccttcaaa catgtattca tattcaccca
     8101 gccggcgacc attggtcagt gtcacatgca tttcttgcaa gggtggcaca aacatgaact
     8161 ggtacataaa actcaacagg tcttgcgtat tcacacgcaa atccagcctt tgctcacctt
     8221 tgctcgtttt caaggtgatg atattgattt cccaatcaaa tgttgcttca taggttttac
     8281 tggctcggct gccaaaagca tagcggtaaa aatgtggttt taatccatgc gggctgattt
     8341 caccctggct ttgttgttcc agcgatggat agagtaattt taacagcccc ttcccctcga
     8401 ccagccaacg caaactgtaa tggccggagt cgtctcgcag atactcaatg ttggcattgc
     8461 ctgccggtcg ctcttcacca ttcacgaaaa tggaaaagtc agtactcaca tgctggtacg
     8521 gcgctggttg agactcatca ggcaccacat caggatgatc catctcgtgc catgccgcga
     8581 tttctgccag ccttgcctgc tgctcttctt cgctcagcgc tggcggctgt ggctgctgtt
     8641 caaccgcttg cacttctgat gctgcatcat caacgggtac ggcttcaact ggcggctcga
     8701 catttgacgt gactactggt tgcggtgcct gtaccggttg agttttcaca ggttttggct
     8761 tgactggtga tggcaatgat ttagccaatg gttgcggcgg aggcaataaa cgcgcctgca
     8821 acacctcagg ctcatgcttt tcacttaaaa accagtcccg ccagtcgacc tcgctaaaaa
     8881 cgaacacatg cagcaacaag gaaaggcata acgccagtgc taatggccag gaaacatgct
     8941 gtcgcatcca gtgttgcagg cctgatgatt gcatcacgcg ctccagggca acgcccttat
     9001 tggccggtga tgctggtgat cacctgctcc atgtgcttac catcatcttc aaccaccagt
     9061 ttcactggca ggtaattctt atccttggcc agataaagcg ttttctcacc ttcagcgtcc
     9121 gtcagtttca ccacattaaa cgtacccgcc tccgtttcca gcggggcact ttcctcagtc
     9181 accacaaagg catgcgggga caatttttta ccattggcca ccgcaagatt aacctgctta
     9241 cctttgggag gttgccacat ccagcagtac attacactta ataaatcctg cgtccctttg
     9301 gtcagcgtgt ccgtctcctg ctcatcttta atctgcatct tgagcacttt tttcgcccag
     9361 tcaaactcac tgatcaatgc cttattaggg cgcttggact ggtgcgactc aaaacggtcc
     9421 ggacgcaaag cattactgat gatacggccc tggcttgaaa gtgtgcgctc acccattagc
     9481 ttgtaaatgc ctttaccttc tgccaaagac tgtatctggt aacggttacc ggtcacactc
     9541 aactgatcct ggacgctacc aatactgatc ccttgttgca gcaggtcata ctttagcgtg
     9601 agctgcttgg gggcagccga caacaccggt gatgccagcc acaggatggc aagcagcaca
     9661 ctggcgatca aacgccccaa agtgtgtcgt ccaagaaaca ccgttacacc accacagtgg
     9721 gcgcttcgcc cgcctgttgc gcacggatct ggccaatacg gtaaacggtc tcacctgcac
     9781 cagccagcac agcctgtgcc tgttcagcat gttccgccgc aacaatcacc gccatgccaa
     9841 ttccgcagtt aaacgtacga tacatttcga gcggttcaat attaccctgc acctgcaacc
     9901 actggaacaa tggcggcaag gtccagctcg acttctgcac ttctgcggtg aggccttctg
     9961 gcaacacacg cggaatattc tcagtgatac caccaccagt gatatgtgcc atccctttga
    10021 ctggcaaggt ttccagtaac ttgaggatgg acttcacata cagtttggtt ggggccatga
    10081 ccacgtcttt aaaactgcgg ccatggaaat cagattccat atcaatgcct gacttgtcga
    10141 tcaacttgcg gatcagggag taaccattgg agtgtgcgcc gctagacgcc aggcccaaca
    10201 ccacatctcc ggctttgatt gtagagccat cgataatatt ggctttgtct acacagccaa
    10261 cagcaaaccc tgccaaatcg tattcgccgg ctgggtacat gccaggcatt tccgccgtct
    10321 caccacccac caacgcacaa ccagactctt cacaaccttg ggcaatgcct ttgatgacgg
    10381 ctgctgcagt gccgacttcg agtttgccac aggcaaaata gtcgaggaaa aacagtggct
    10441 cagccccttg caccagaatg tcgttcacgc tcatggcgac cagatctatg cccacggtat
    10501 cgtgcttgtt caactggaag gccagcttga gtttggtccc cacgccatcg gtaccagaga
    10561 ccagcacagg ctccttgaat tttttaggca tggcaaacag ggaaccaaaa ccaccaatac
    10621 cacccatcac ttctggacgc agtgtgcgtt tggcaaatgg cttgatctgt tctaccaggg
    10681 catcaccggc ctcgatatcg acaccggcat cgcggtaggt gatagaagtt ttatcagaag
    10741 aagtcgtcaa gatactctcg tttctttcaa tgcgattaat caggtgaacg gctgtaaaca
    10801 gcgcagatgt ttgcaacaaa gcgcgataca gcgttgaaat tcagtataat tttaaaataa
    10861 tcattatttt aacgcatctt gccacttcga cctatgccgg attgcggatg tgtgtgacag
    10921 gtgcccatgt ctgaccaaga acatcagaag tccattgcga cccaatttgt cagcgaccat
    10981 cgctggtgga tcgtaaccgc cgtcgttatc tacctgtttt atttactgga acctatcctg
    11041 actccttttc tggccgcagc cgtgatcgcc tatatgctgg acccgctggt cgacaagttg
    11101 agcgaagtcg gctaccgcaa tcggcaaatt ggccgcacgc tggcaacctt gctcgtcatg
    11161 tctggcgtga tcctggtcat gatcggtttg ctgctgatta ttatcccgct gctgcaacag
    11221 caatcgaccc tgattgcaca acgcctgcca ttgttattgg atcactttca ccagcaagtc
    11281 gaaccttggc tgctacagcg ttttggcatt cacctgaata ttgatcgcac agacatccaa
    11341 aagcttatta gcgaacactg gcagaccgcc ggtagcatga taggcaacgt gctactcagc
    11401 gccggacaaa aaggattaaa cctgattggc atgattgcca atatcttgct gctgcccgtg
    11461 gtcctgtttt atttcctgcg cgactgggat gacatgatgg ccgaaattgg tgacctgatc
    11521 ccgcgtaact ggataggccg gatcaaagca ctgtgcaagg atatcgatag tgtcgtcgcc
    11581 gagtttttac gtgggcaatt gtccgtcatg ctgtcgttgt gcgtgttcta cagtataggc
    11641 ttgtggctgg tcggcctgga tatggccctt tccatcggca tgattgccgg cctgctcagc
    11701 tttgtacctt acctcggctt cgcactggct tttatcctgg ctgtgttact ggccctactg
    11761 caatttgcct cactccctga agtcatccca gtattactgg tgtttggcat cggccaattt
    11821 gtcgaaagct ttttcctgac acctatcctg gtgggtgacc gcattggctt gcatcctgtg
    11881 ttggtgatcc tggctttaat ggctggtgga caattgtttg gctttgctgg tgtattgctg
    11941 gcattgccag tcagtgccgc gattgccgtc ggcgtgcgcc acaccaaaca aagctacctg
    12001 cgtagcgaag cgtatttgaa tagtccgcct catatcattc aaacgctgga tgaatgattg
    12061 cctaaacacc agaaattagc gctgtgaaac aactgttact gaatatccaa ccacctgctg
    12121 cgccagggtt tgataatttt attgtcggcc aaaaccaaga agcactggct agcgtgcgat
    12181 cggtacttgc tgacgctgcc accacccgtt ttgtttacct gtggggagaa tctggcagcg
    12241 gcaaaagcca tttactactg gctgcccagg ccatgggtgc gcatattgca gatgatgtac
    12301 acaccctgga tgaagagtcg caaatcatct tgtttgacac ctacaaccag atcaaggctg
    12361 aaggaggcgt attgatcacg gcaggaagcc acgcccccac acaaatgggg ttacgcgcag
    12421 atctggctac ccgtctggcc tggggtctgg tttaccagtt actgccattg agtgatgatg
    12481 agaaggcgga agcactgcgt gcccacgccc gtgaccgtgg catgaaactc cctgacgaag
    12541 tgatcgctta ctgcctgcgt tatttacgcc gtgatttacc gaccctgatg gctgtgttag
    12601 aagcgcttga tgagtggtca ctgaccacca agaaagcagt tacggtgcaa tccctgaaga
    12661 aaatgctgca agccacgtta tagcaccata tagcgattat tccggcccaa aactatacct
    12721 gtttttgcct ttctttttcg agtcatacat ggctttatct gccacatgta atagctgctt
    12781 cacacttttt ccatgatgcg gatattcggc aatgccaatg ctgacgccga tttccaacgg
    12841 aatcccatta acgatcaccg gttgtgcaaa caactgtatc aaccgatgtg ccagctgcgt
    12901 caactcgagg cgatataagt aatttcccac caccagcaca aactcatcac ctccttgccg
    12961 tgccagaaaa tcagattgcc tgagcacttt tttaaacata ctggcaactt caatcaataa
    13021 cgcatcgccg acttcatggc cgtatttgtc gttcaccagt ttgaaaccat ccagatccat
    13081 gaaaagaatc gcaaactgct gtgacgcatg tctggccatt tgataagtgc tcgccagatg
    13141 cacatccaca ctgcgccggt ttggcaagcc agtcagcaag tcatgacccg cctgatgact
    13201 ggcttgttta gccaccgcct gattttcaat cacctgttct aacaaatggg ttgtgcttcg
    13261 gtaagaaaac cacaacacgc caataatcaa tatgcccaac agaatcgccc ccagcagcga
    13321 ctcaaacatg cgttggcgga tctcttgctc aaaatttacg cgcaaatgct caagcttcat
    13381 gtcaatcgtt gccaactgat cattgatcag cttcattaat tcacgacttt tatccttgct
    13441 cagtgagagg tgagaagcgt actccccggc atgcagctgc acctgaatac tctggtccat
    13501 cacctcaaac ttttgatgga cgagccgctt gatcttttct aatgtcggtt tcacttcctg
    13561 aaagttcttc gactgccttt ccagacgcga gatggctttt tcggtctcgg cccgtccctg
    13621 ctccaggcta tccaagaaca acatgtggcc tgtcagcaaa tatcctcgtt ggccattttc
    13681 agcatcggta atggctttac cca
//
LOCUS       DATMSK010000219         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_463246,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000219 DATMSK010000000
VERSION     DATMSK010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463246"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..590
                     /locus_tag="VNZ84_07385"
     CDS             84..590
                     /locus_tag="VNZ84_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWU66817.1"
                     /translation="MKKNSYLQTGFTLVEMVIVILIMSALAVTAYARISQIDTQARLA
                     ALQSFKASVLSVATMAKGVCLSDAQCQSTQSQGTPSTTIDGNTIYFTYGYPVGWLADN
                     EDGSGSLHQLLDPGKFVIQPSLSSLNRATYFLSGARDMDHCKLEYSIAGGTPSASGIT
                     VNIDSSGC"
     gene            complement(663..1889)
                     /gene="fabB"
                     /locus_tag="VNZ84_07390"
     CDS             complement(663..1889)
                     /gene="fabB"
                     /locus_tag="VNZ84_07390"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HWU66818.1"
                     /translation="MRRVVITGFGIVSSLGNNKQEVLDSLKAGRSGIAYQPEYAERGL
                     RSHVAGSIKNLDIEALIDRKLLRFMAKGHAYAWLAMQEAIADASLPEELVSNVRTGLI
                     VGAGGTSTESMLEGTNTLAEKGIRRVGPYMVTKTMSSGIAACLATGAKIKGVNYGISS
                     ACSTSAHCIGAGVEQIQLNKQDIVFAGGGEEEHWTMSYLFDAMGALSSKYNDTPEKAS
                     RTYDADRDGFVISGGGGIVVLEEYEHAKARGAHIYAEVIGYGATSDGYDMVAPSGEGA
                     VRCMQQALQGINPEDIDYINTHGTSTPVGDTKELEAIRAVFGSHSKTAIASTKSLSGH
                     ALGAAGVNEAIYTLLMMQHGFIAASANIDNLDPAAEGLNIVRTPIASAKVQTALSNSF
                     GFGGTNATLTLSTKNL"
     gene            complement(1902..2417)
                     /gene="fabA"
                     /locus_tag="VNZ84_07395"
     CDS             complement(1902..2417)
                     /gene="fabA"
                     /locus_tag="VNZ84_07395"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HWU66819.1"
                     /translation="MTSKSSFSKEELLACGRGEMFGEGNAQLPLPPMLMFDRIVKISD
                     EGGKYGQGEIIAELDIRPDLWFFECHFTGDPVMPGCLGLDAMWQLVGFFLGWKGGPGR
                     GRALGSGEVKFTGQVLPTAKLVRYHIDLKRVIMRKLVMGIADARMEVDGREIYNASDL
                     RVGLFTTTDNF"
BASE COUNT          689 a          722 c          556 g          647 t
ORIGIN      
        1 gcatttgtaa gacttggtta acagttgcaa cattcaacaa aagttgacat gcatgcccac
       61 actgtacgat aagatcgtac acaatgaaaa aaaattccta ccttcaaaca ggctttacgc
      121 tggtcgagat ggtcattgtg atactcatta tgtcagcctt ggcggtgact gcctatgccc
      181 gtataagcca aattgacacc caggccaggc tggcggcatt gcagtcattt aaagcatcgg
      241 tattatcagt cgcgacgatg gccaagggtg tgtgtctgtc agatgcacaa tgccagagta
      301 cacaaagcca gggaacacct tctaccacca ttgatggcaa caccatttac ttcacttatg
      361 gctatccggt aggttggctg gccgataatg aagatggcag cggaagcttg caccaattac
      421 tggacccggg caagtttgtc attcaacctt ccctctccag ccttaatcgt gcaacttact
      481 ttttatctgg tgcacgcgac atggaccact gtaagctgga atacagcatc gctgggggta
      541 cgccctctgc atctggcatc accgtcaata tcgatagcag cggctgttaa gttttaaatc
      601 gaaataaaaa aggagtgcgc ctattcttaa gcacactcct tcaattgggt taacacgacg
      661 acttacagat ttttggtcga cagtgtcagc gttgcattgg taccgccgaa gccaaagctg
      721 ttagataacg cagtttgaac cttggcagaa gcaatcggcg tacgcacaat attcaagcct
      781 tcagcagccg gatccaggtt atctatattg gcagaagccg caataaaccc atgttgcatc
      841 atcagcaaag tataaatcgc ctcattcacg ccagccgcac ccagtgcatg accggacaat
      901 gacttggttg aagcaatcgc cgttttgcta tggctgccaa atacggcacg gattgcctca
      961 agctccttgg tatcacccac gggcgtactg gtaccgtgtg tattgatgta atcaatgtct
     1021 tccggattaa taccctgtaa tgcctgttgc atacagcgta cggccccttc cccgcttggc
     1081 gcaaccatgt cataaccatc cgaggtcgca ccatagccaa tcacttcggc gtagatatgc
     1141 gcaccacgcg ccttggcatg ctcatactct tcaagcacca caataccgcc accaccagaa
     1201 atcacaaagc catcacggtc tgcatcataa gtccgagaag ctttttctgg tgtgtcgtta
     1261 tacttgctag agagtgcacc catagcatcg aacaggtagc tcatggtcca gtgttcttct
     1321 tcaccaccac ccgcgaagac gatgtcctgc ttgttgagtt gaatttgctc taccccggcg
     1381 cctatgcaat gcgcactggt tgaacaagca gaactgatgc cgtaattgac gcccttgatc
     1441 tttgcgcccg tagccagaca ggccgcaatc ccgctgctca tggttttggt caccatataa
     1501 gggcctacgc ggcgaatgcc tttttctgcc aacgtattgg tgccttccag catgctttcg
     1561 gtagaggttc caccagcacc cacaataagg ccagtacgta cattagacac caactcttcc
     1621 ggcaagctgg cgtcggcaat tgcttcctgc atggccagcc aggcatacgc gtggcccttg
     1681 gccatgaaac gtaacagttt gcggtctatc agggcctcga tatccagatt cttgattgac
     1741 ccggccacat gcgaacgcaa accgcgctcg gcatattccg gttgatacgc aataccagac
     1801 ctgcctgctt tcaggctatc cagtacttcc tgtttgttat taccaagact tgatacgata
     1861 ccaaatcctg taatcactac gcgacgcatt gcgcctcctt actagaaatt gtccgtggtg
     1921 gtaaacaacc caacacgcaa atcactggca ttataaattt cgcggccatc cacttccata
     1981 cgggcgtcag caatacccat caccaacttg cgcataatca cacgctttaa atcaatgtga
     2041 taccgcacca gcttcgcagt cggcaaaacc tgtccagtaa acttgacttc acctgaaccc
     2101 aaggcacggc cacgacccgg gccgcctttc caacccagaa agaaaccaac cagctgccac
     2161 atggcatcga ggcccagaca gccaggcata acaggatcac cggtaaaatg gcactcaaag
     2221 aaccacaaat ccgggcggat atccagttct gcaatgattt cgccctgacc gtattttcca
     2281 ccttcatcag aaatctttac gatgcggtca aacatcagca tcggtggcag tggcaactgt
     2341 gcgttacctt cgccaaacat ctcaccgcgg ccgcaagcca gcaactcctc tttactgaaa
     2401 ctacttttac tagtcatgta tttttagatt tttagtataa aacaatattt tcgccggaaa
     2461 ttgcgtttag tgaaagcttt ttgtcacccc acaactatac tgacctgtat acttttattt
     2521 aaaacacagg taagattcct gaaattgtta ctgaatttta catccgccag aatggcaata
     2581 gcacagggtt taaaacagga ttgatgaatt tttc
//
LOCUS       DATMSK010000220         5684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_463264,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000220 DATMSK010000000
VERSION     DATMSK010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463264"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..2362
                     /gene="katG"
                     /locus_tag="VNZ84_07400"
     CDS             176..2362
                     /gene="katG"
                     /locus_tag="VNZ84_07400"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HWU66820.1"
                     /translation="MENKQNTAGKCPFGHGALTASGNSNTGWWPSALNLDILHQQDRK
                     TNPLGEAFDYRKEVSTLDVDALKKDLHALMTDSQAWWPADWGHYGGLMIRLTWHAAGT
                     YRVADGRGGAGTGNQRFAPLNSWPDNVNLDKARRLLWPIKKKYGNKLSWADLITFAGT
                     IAYESMGLKTFGFAFGREDIWHPEKDIYWGNEKEWLAKTGSEGSRYSGQRDLENPLAA
                     VMMGLIYVNPEGVDGNPDPLKTAQDMRVTFARMAMNDEETVALTAGGHTVGKAHGNGQ
                     AGNLGENPEAADVHEQGMGWNNHVSRGIGRDSVTSGIEGAWTTHPTKWDNGYFAMLFN
                     HDWALQKSPAGAWQWEPTNIKEEDKPVDVEDPSIRYNPIMTDADMALIKDPEYRKISE
                     RFRDDQAYFSEVFARAWFKLTHRDMGPKARYIGPDVPQEDLIWQDPVPAGNGNLNDAD
                     IAILKTHILDSGLTTAELVATAWDSARTFRGSDYRGGANGARIRLAPQKDWAGNEPAR
                     LQKVLAVLESIQKQFAKPVSIADLIVLGGSAAIEQAAKAAGVAVTVPFTPGRGDATAE
                     QTDTESFEVLEPVHDGYRNWLKGDYVPTPEEMLLDRTQLLGLTAPEMTVLIGGLRVLD
                     TNHGGSRHGVFTDRPGVLSNDFFVNLTDMHYTWVPAGKNLYEVRNRTTGLVKWTATRV
                     DLVFGSNSILRAYAELYAQDDARQKFVQDFVAAWTKVMNAERFDLK"
     gene            2467..3270
                     /locus_tag="VNZ84_07405"
     CDS             2467..3270
                     /locus_tag="VNZ84_07405"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWU66821.1"
                     /translation="MDIYLMLKAVIMGLVEGATEFIPVSSTGHLIITGEFLDFLSKDK
                     RDVFEIIIQLGAILAVCVEYRQRLWNTVQRIQTDTNAQHFVRNLIIAFLPAALIGLAL
                     HHQIKQYLFSSFTVGIALVVGGILILLIEKFAPAGRTTEVDQITAKQALQIGFAQSLA
                     LFPGMSRSGATIMGGMCFGLNRQTATEFSFFLALPIMFAATGLDLYQAHELLSADDAL
                     IFLIGFVISFLSALVVIRALIRFVAQHSFVVFAWYRIVFGILVLLFFAH"
     gene            3290..>5684
                     /locus_tag="VNZ84_07410"
     CDS             3290..>5684
                     /locus_tag="VNZ84_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWU66822.1"
                     /translation="MNRALFKKIAFGIAAILALLVLAVVYLAVTFNPNDYKPTVIQLV
                     KDKKQRTLDIKGDIKLSFWPKIGADLGEITLSEHQSDRQFAAIKSAKVALAVLPLLKS
                     EIVVDTVYLDGAQVNVIQHADGSFNFDDLLSKEEEESKQINFDVQGIKITNTQASFIN
                     EGTGAKYSVDQLNLTTGQVALKKPFDVATDFHMMASQPAVDAKAAIKASIMADPEGKH
                     FVVKDLDAQLKGMLLDGQDVTVTAQGSVDVDAAKTALDVSGLKLAMQGDFKGAQRDVS
                     LHAPALKVNPALISSDKVLLSLKQKDANGDLDLAVVLAELKGNQQAVESKGITADVSL
                     NAGARKVEGHFASPVKANLADLVFEVPALAGKFDIKDPALPNGAMQGQFKLAANANIK
                     QEQVKTTFDLSLAETKLNGDVIVAGFSTPHIGFKVHADTLNLNALLGNTANKKEAAVS
                     SSKASSRKADKPADLSALKTLFLDGSINIGKIMYSPYTLSGLNVGIKADGQKLSLQGL
                     DVKLDDTRIQGNIGISQFSKPLYMFDLNIDKLDLNRYLPASEAKATESKAAEKTANSA
                     EQPIDLSALKALNAQGNIRIGWLKYGKTEAKNLNIGLKAQDGLASLDPLNVDVYQGTV
                     RGSVRLDARATPAVTIQQNLQNIAVGPMLVDTINNDMLSGNGNLNLNVTAQGNTVTAL
                     KKSLGGSVDLRMADGAVKGFDLAGAIRDAKGKLNVLKGQSNADADKSKKTDFSELTAT
                     FNIKNGIAHNDDLAMKAPVFRLTKGDSKGDIDIGKEQINYLAKPTLVNSLKGQGGKDA
                     "
BASE COUNT         1491 a         1245 c         1512 g         1436 t
ORIGIN      
        1 tgtttacctg gctataccac gaacaatctg cattagagga aatgttggcg gcttacgctg
       61 ccagtgttta agccaattta ttttgtagtg aatgataatt gcaatattta acaagtagta
      121 tatttatcat ctggacattt cttgcgtaat ggccagctat caaaaggagc tcaacatgga
      181 gaataagcag aatacggcag gtaaatgtcc ttttggacat ggggcactga cagcctctgg
      241 aaattctaat actggctggt ggccaagtgc cctcaatctc gatatcttgc atcagcaaga
      301 ccgcaaaaca aacccacttg gcgaggcatt tgattatcgt aaagaagtgt ccaccctaga
      361 tgtagatgca cttaaaaaag acttgcatgc attgatgaca gatagtcagg catggtggcc
      421 agcagattgg ggacattatg gaggcttgat gattcgcctg acctggcatg ctgccggtac
      481 ctatcgtgtc gctgatggcc gtggtggtgc cggaacaggt aaccagcgtt ttgcgccact
      541 caatagctgg cctgataacg tgaacctcga caaggcgcga cgcctgctct ggccgatcaa
      601 gaaaaaatac ggtaataaac tgtcttgggc agacttgatt acgtttgccg ggaccattgc
      661 ctatgagtct atgggcctga aaacttttgg atttgctttt ggccgggaag atatctggca
      721 tccagaaaag gatatttact ggggcaatga aaaagagtgg ttagcgaaaa ccggcagtga
      781 aggcagccgt tactctggtc agcgcgacct tgaaaatcca ttggctgccg tgatgatggg
      841 cctgatttat gtcaatccag agggggttga tggcaatcct gacccattaa aaacagcgca
      901 ggatatgcgg gtcacttttg ctcgtatggc catgaatgac gaagaaactg ttgcattgac
      961 cgcaggaggc cataccgtcg gaaaagcaca cggcaatggt caggctggta atttaggaga
     1021 aaatccggaa gctgctgatg tacatgagca aggcatgggc tggaacaatc atgtcagccg
     1081 cggcattggt cgtgactctg tgaccagtgg gattgaaggt gcctggacga cgcatccgac
     1141 caaatgggat aacggttact ttgccatgct gtttaatcat gactgggcac tacaaaagag
     1201 cccggccggt gcctggcaat gggaacccac gaatatcaag gaagaagaca agccggtcga
     1261 cgttgaagat ccttcaatac gctataaccc gatcatgacc gatgctgata tggccttgat
     1321 taaggatcca gagtatcgaa aaatttctga gcgattccgc gatgatcaag cttatttttc
     1381 ggaggtgttt gcccgtgcct ggttcaagct gacgcaccgt gatatggggc cgaaagcccg
     1441 ttatattggg ccggacgtgc cacaagaaga ccttatctgg caagacccgg tacctgctgg
     1501 taatggcaat ttgaacgatg cggatattgc cattttaaaa acacatattc tggacagtgg
     1561 attaactact gccgagctgg tggccaccgc ctgggatagc gcacgtactt tccgcggatc
     1621 tgactatcgt ggcggggcca atggtgcgcg gattcgcctg gcaccgcaaa aggactgggc
     1681 aggcaatgaa cccgccaggt tacaaaaagt gttggctgtt ctggagagta tccagaagca
     1741 atttgccaaa cctgtcagta tcgctgactt gatcgtgttg ggcggatcgg ctgcgattga
     1801 acaggcagcc aaagccgcag gtgtggcggt gaccgtaccc tttaccccgg gccgcggtga
     1861 cgcgacagct gaacagacgg ataccgagtc attcgaggtg ctggaaccgg tacacgacgg
     1921 ctaccgtaac tggctcaaag gcgattatgt cccgacacca gaggaaatgc tgcttgaccg
     1981 gacacaactg ctgggcctga cagcacctga gatgactgtg ctgattggcg gcttacgtgt
     2041 gctggatacc aaccatggtg gtagcaggca tggcgtgttt actgatagac caggcgtgct
     2101 cagtaacgac ttttttgtca atttgacaga catgcactat acatgggtgc ctgcaggtaa
     2161 aaacctgtat gaagtgcgta accgcactac gggtttggtg aaatggactg ccacccgggt
     2221 cgatctggtg tttggctcta actcgatact gcgtgcctat gcagaattgt atgcacaaga
     2281 cgatgcccgg caaaagtttg tacaagactt tgtcgctgcc tggaccaaag tcatgaatgc
     2341 agagcggttt gatctgaagt aactagtttt gagcacaaag caggcgcctt gttcacgcat
     2401 ggcgcctgtt gttttatgta aatattgaat catctcactg tcgcattggt ctgttagaat
     2461 acaaccatgg atatttattt gatgctgaag gccgtaatca tgggcctggt cgaaggcgcg
     2521 accgagttta ttcccgttag tagtactgga catttgatca tcaccggcga gtttctggat
     2581 tttctctcca aagacaagcg tgatgtgttt gaaatcatca ttcaacttgg cgccattctg
     2641 gcagtctgcg ttgaataccg gcagcgttta tggaataccg tgcaacgtat tcaaactgat
     2701 accaatgcgc agcactttgt ccgtaacctt attattgcct ttttgcctgc tgcactgatt
     2761 ggcttggcgc tacatcatca aattaaacag tatttatttt cttcgttcac cgttggcatt
     2821 gcgctggtgg tcggtggtat cctcatttta ctgattgaaa aatttgcacc tgctggtcgt
     2881 accacggagg ttgaccagat cacggccaag caggcattac agattgggtt cgcccaatcg
     2941 ctggccttgt tccctggcat gtcccggtca ggcgctacca tcatgggggg gatgtgtttt
     3001 ggcctgaacc gtcaaaccgc caccgagttc tcattctttc tggccttgcc aattatgttt
     3061 gctgccacgg gcctggattt ataccaggcg catgaattat tgtcggcaga cgacgcactg
     3121 atctttttaa ttggttttgt aatctcattt ttatctgcct tggtggtgat acgtgccctc
     3181 atccgctttg tagcccagca ttcatttgtg gtatttgcct ggtatcgcat cgtttttggc
     3241 atcttggtat tgttgttttt tgctcattaa cttgtttaag gtgtgagtca tgaatcgtgc
     3301 actgtttaaa aaaatagcgt ttggcatcgc ggctattctg gcactgttag tgctagcggt
     3361 tgtttacttg gcggtgacgt ttaatcctaa tgattacaag ccaactgtga ttcaattggt
     3421 gaaagataaa aaacagcgca cgctagatat caaaggcgat attaagctca gtttctggcc
     3481 aaaaatcggt gcagatttag gtgaaatcac cttgtctgaa catcagtcag acaggcaatt
     3541 cgctgcgatt aagagcgcca aagtggcgtt ggcagttctg cctttactta aaagtgaaat
     3601 tgtcgtggat accgtttacc tggatggcgc gcaggtaaat gtgattcaac atgccgatgg
     3661 cagttttaac tttgacgatt tgctgagcaa ggaagaggaa gagtccaagc agattaattt
     3721 tgatgtacaa ggcatcaaaa tcaccaatac ccaggccagt ttcatcaatg aaggtacagg
     3781 cgctaaatat agcgtggacc agttgaacct gactactggt caagtggcgt tgaagaaacc
     3841 atttgatgtt gccacggact ttcacatgat ggcgagccag ccggcagtag atgccaaagc
     3901 cgccatcaag gccagtatca tggccgatcc cgaaggtaag cattttgtgg ttaaagacct
     3961 ggatgcccaa ttaaaaggga tgttgcttga tgggcaagat gtgactgtga ccgcgcaagg
     4021 ctcggtggat gttgatgcgg caaaaacggc attggatgtg tctggcctca aattggcgat
     4081 gcaaggtgac tttaaaggcg ctcaacgcga tgtcagctta catgcacctg ccctgaaagt
     4141 gaatcctgca ttgattagca gcgataaagt gttgttgtca ctcaagcaaa aagacgccaa
     4201 tggtgatctg gacctggccg tggtgctcgc tgagttaaaa ggtaaccagc aagctgtgga
     4261 aagcaaaggt attacagccg atgtcagcct taatgccggt gcccgcaagg tagaaggcca
     4321 ctttgcttcg cctgtcaaag ccaatttggc tgatctggtg tttgaagtgc cagcattggc
     4381 tggcaagttt gatatcaagg atccggcact gcccaacggt gccatgcaag gccagttcaa
     4441 gttggcggcc aatgccaata tcaaacagga gcaagtaaaa accacgttcg atttaagcct
     4501 tgccgaaacc aaattgaatg gcgatgtcat tgtggctggc ttcagcactc cgcatatagg
     4561 atttaaagta catgcggata cattgaattt aaatgcactg cttgggaata cagctaataa
     4621 gaaagaggcg gctgtttcca gtagcaaggc cagcagccgc aaggccgata aaccagcgga
     4681 cttgagtgca ttgaaaacct tgttcctgga tggcagtatc aatatcggca agattatgta
     4741 tagcccttat accttaagtg gcttgaatgt gggcatcaag gcagatggtc aaaagctttc
     4801 gttgcaaggt ctggacgtca agctcgatga tacgcgcatt caaggcaaca taggtattag
     4861 ccagttttcc aagccgctgt acatgtttga cctcaatata gacaaactgg acctgaaccg
     4921 ttacttgcct gctagtgaag ctaaggcgac tgaaagcaag gcagccgaaa aaactgcaaa
     4981 tagcgctgag cagccgattg atttgtccgc attaaaagcc ttgaatgcac aagggaatat
     5041 acggataggc tggttgaaat atggcaaaac cgaagccaaa aatctgaata ttggtttaaa
     5101 ggcacaagat ggcctggcca gcctggaccc acttaatgtg gatgtgtatc agggcacggt
     5161 gcgaggttcg gtgaggcttg atgcccgcgc cacaccggcg gtaacgattc agcaaaacct
     5221 gcaaaacatc gcggtagggc caatgttagt cgatacgatt aataatgata tgttgtcagg
     5281 taacggtaat ttgaatctga atgtgactgc gcaaggcaat acggtgactg cactaaaaaa
     5341 gtcgctgggc ggctcggttg atttgcgtat ggccgatggc gctgttaagg gctttgatct
     5401 ggctggcgcg atccgggacg ccaaaggcaa gctgaatgtg ctcaaaggcc agtctaatgc
     5461 cgatgcagat aaaagcaaaa agacagattt tagtgagctg acggcaacgt ttaatattaa
     5521 aaatggcatc gcccacaatg atgacttggc catgaaggca cctgtttttc gtttgaccaa
     5581 gggcgacagc aaaggtgata ttgatatcgg caaagaacaa attaattacc tggccaagcc
     5641 aacattagtt aactcgctca aggggcaggg tggtaaggat gcag
//
LOCUS       DATMSK010000221         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_369476,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000221 DATMSK010000000
VERSION     DATMSK010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369476"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..560)
                     /locus_tag="VNZ84_07415"
     CDS             complement(75..560)
                     /locus_tag="VNZ84_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HWU66823.1"
                     /translation="MKRYVLIALSGLLVACQQPQQQTVSNQPQSAVEVKSQVQTLNLQ
                     SLSSGRSTEDNAAVKQVFAQKQSHVWLEGHGIVKKLLPDDTKGAKHQRFLVTIAPEQT
                     LLFAHNIDLARRAEVKVGDQVQFRGEYIYNPKGGIMHWTHHDPQGRQGGWIKLNGQTY
                     E"
     gene            complement(557..1582)
                     /gene="trpD"
                     /locus_tag="VNZ84_07420"
     CDS             complement(557..1582)
                     /gene="trpD"
                     /locus_tag="VNZ84_07420"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWU66824.1"
                     /translation="MAITPKIALQRLIDHTDFTHEEMLAIMQQIMSGEFTQIQIAGFL
                     SALRVKGETVTEIAAAAQVMRELSSKVDISDTRHLIDTCGTGGAPNKVFNVSTASAFV
                     AAGAGAKIAKHGGRAASSKSGSADVLEALGVNIVLTPEQVVRCVNEAGIGFMFAPNHH
                     AAMKYAAPVRRELGVRTMFNLLGPMTNPAGAKRQVMGVFHRDLVPLLAQTLQQLGSEH
                     VMVVHSADEMDEISFSADTYVAELKDGTIREYTLNPSQFGMPLHNINSIRVESAQHSS
                     EIILGLLSGEKGPARDIVLLNAGAAIYVSGLAADLTTGIAQAAKSIDSGAALNKLQQL
                     KALSQAA"
     gene            complement(1582..2148)
                     /locus_tag="VNZ84_07425"
     CDS             complement(1582..2148)
                     /locus_tag="VNZ84_07425"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWU66825.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGQDVHVHRNDEITLEQIKAMKP
                     EKIVISPGPCTPNEAGISVPLIHEFAGKIPLLGVCLGHQSIGQAFGGKIIKAKTLMHG
                     KTSLIHHTNTGVFRNLPNPYTATRYHSLVIERETIPDCLEITAWTEDGEIMGVKHKTL
                     AVEGVQFHPESILTEYGHELLDNFLKGY"
     gene            complement(2150..>3193)
                     /locus_tag="VNZ84_07430"
     CDS             complement(2150..>3193)
                     /locus_tag="VNZ84_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HWU66826.1"
                     /translation="EEIAVVDNLSGKLYFIVYADPSKANAYETACARMHTLVEKLRCS
                     VEIPTALPGAKTAAISEFGEDNFKAAVKKGQQYILEGDIMQVVLSQRMVQDFAAPPLS
                     LYRALRSLNPSPYMFYYDMGDHHVVGASPEILVRLEDTTVTSRPIAGTRPRGKNRDHD
                     MALEAELLADPKERAEHVQLMDLGRNDVGRVALTGTVKVTDNMTIERYSHVMHIVSNV
                     EGQLKPGLDAIDVLKATFTAGTVSGAPKVRAMEIIEELEPSKRGIYAGAVGYLGFNGD
                     MDVAIAIRTAVIKDSKLYVQAGAGIVADSVPQSEWEETQNKAKAVIRAAELVQAGLDS
                     QAANIQTHAGKGA"
BASE COUNT          770 a          861 c          749 g          813 t
ORIGIN      
        1 atgccgccgc atatcattaa tatgtaaggc agaatttttt aatggcaaca acgtatcgtg
       61 actaaagttt agttttactc gtatgtctgt ccgtttaact taatccaacc accttgacgg
      121 ccctgcgggt catgatgcgt ccaatgcata atgccgccct tggggttgta aatatattca
      181 cctctaaact gcacctgatc gcccactttg acctcagcac gccgcgccaa atcaatattg
      241 tgcgcgaaaa gtaatgtttg ctcaggcgca atcgtaacca ggaatcgttg atgttttgca
      301 cccttggtat cgtctggtaa cagttttttg acgatgccat gaccttctag ccagacatga
      361 ctctgctttt gcgcaaatac ctgcttaact gccgcgttat cttccgttga gcgtccagaa
      421 gaaagcgatt gcagattcag tgtctgaacc tgtgatttca cttccacagc ggactgtggt
      481 tgattgctga cagtctgctg ctgcggttgc tggcatgcca ccagcaaacc ggacagcgca
      541 atcaatacat aacgcttcat gccgcctgac ttaaggcttt cagttgctgc aacttattca
      601 aggctgcacc tgagtcaatg gattttgccg cttgtgcaat gcctgttgtt aaatcagccg
      661 ccaagcctga gacataaata gcggcaccgg cattcagcaa cacaatgtct ctcgccgggc
      721 ctttttcgcc agacagcaaa ccaagaataa tctcgcttga atgctgtgca ctttccaccc
      781 gaatactgtt gatattatgc aatggcatgc caaattgcga cgggttcaaa gtatattcac
      841 gaatcgtacc gtctttaagt tcagccacat aggtatctgc cgaaaaggag atttcatcca
      901 tttcgtctgc actatgcacc accatcacat gctcactgcc taattgctgc aaggtctgcg
      961 ccagtaatgg caccaaatcg cgatggaaca cccccatcac ctggcgcttg gcaccagctg
     1021 gattcgtcat tggcccaagc aaattaaaca tggtacgcac ccccaattca cgacgcacgg
     1081 gggcagcata cttcatggca gcatggtggt tgggcgcaaa cataaagcca ataccggcct
     1141 cgttgacgca acgtaccacc tgttcaggcg tcaacacgat attcacgccc agcgcttcca
     1201 gcacatcagc tgagccactc tttgaagacg ctgcacggcc accatgtttg gcgatttttg
     1261 cacctgcccc tgcggcgaca aaggctgagg ccgttgaaac attaaagact ttgttaggcg
     1321 caccgccggt accacaagtg tcaatcaaat gacgcgtatc actgatatcc actttgctgg
     1381 agagctcacg catgacctgt gcagcagcgg caatttcagt gaccgtttct cccttgacgc
     1441 gcagcgcaga caaaaagcct gcaatctgga tttgggtaaa ctcaccactc atgatctgct
     1501 gcatgattgc cagcatttct tcgtgcgtga aatcagtatg gtcaatcagg cgctgcagcg
     1561 caatttttgg cgtgatggcc attaatagcc tttcagaaag ttatccagca gctcatgccc
     1621 atactcagtc aaaatagact ccgggtgaaa ctgcacgcct tctacagcca aggttttatg
     1681 cttgacgccc atgatctcac catcttcagt ccaggcggta atctccagac aatctgggat
     1741 agtctcacgc tcgatgacca gcgaatgata gcgtgtggca gtatatgggt taggcagatt
     1801 tctgaacaca ccggtgttgg tatggtggat cagagaagtt ttaccatgca tcagggtttt
     1861 cgccttgatg atcttgccgc caaacgcctg accaatgctc tggtggccca gacacacgcc
     1921 caacaggggt atcttgccag caaactcatg aatcagcggc acagaaatac ccgcttcgtt
     1981 gggtgtacaa gggccaggag aaatcacgat tttttcaggc ttcatcgcct taatctgttc
     2041 gagtgtgatt tcatcatttc tgtgcacatg tacgtcttgc cccagctcgc caaagtactg
     2101 tacgaggtta taggtaaaag agtcgtagtt atcaatcatc aacaacatat caggctcctt
     2161 ttcctgcatg ggtttgaata ttggctgcct gactatccaa gccagcctgc accagctcag
     2221 ccgcacgaat caccgctttg gctttgtttt gggtttcttc ccattcgctc tgcggcacgg
     2281 agtcggccac aatgccagcg ccagcctgca catacagttt gctgtcttta atcacggccg
     2341 tacggatggc aatcgccaca tccatatcgc cattaaagcc caggtaaccg acggcacccg
     2401 cgtaaatccc gcgcttggaa ggctccaact cttcgataat ttccatggcg cgcactttgg
     2461 gagcaccgga tacggtccct gcagtgaaag tcgccttcag tacatcaatc gcatccaggc
     2521 caggtttgag ctggccttcg acattgctga cgatatgcat cacgtgcgag tatcgctcaa
     2581 tggtcatgtt gtcagtgact ttaacagtac ctgtcagtgc gacgcgaccc acgtcattac
     2641 ggcctaaatc catgagttgt acgtgctcgg cacgctcttt ggggtcggcg agtaattcgg
     2701 cttctagtgc catgtcgtga tcgcggtttt taccacgcgg ccgtgtacct gcaatcggac
     2761 gtgaggtcac ggtggtatct tccaggcgca caaggatttc tggtgaagca cctactacat
     2821 gatgatcgcc catatcgtaa tagaacatat agggcgatgg gttcaggctg cgcaaggcac
     2881 ggtacaaact tagtggagga gcggcaaagt cttgcaccat gcgctggctg agaaccactt
     2941 gcatgatgtc gccctcaaga atatattgct ggcctttttt aaccgccgcc ttgaaattgt
     3001 cttcaccaaa ctcggaaata gccgctgttt ttgcaccagg caatgccgtg ggaatctcaa
     3061 cgctgcaacg taatttttcc accaaagtat gcatgcgcgc gcaagcagtt tcgtaggcgt
     3121 tggctttaga cggatcagcg taaacgatga aatatagttt gccggagagg ttatccacca
     3181 cggcaatttc ttc
//
LOCUS       DATMSK010000222        11843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_369773,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000222 DATMSK010000000
VERSION     DATMSK010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11843
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369773"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..2874
                     /gene="nirB"
                     /locus_tag="VNZ84_07435"
     CDS             295..2874
                     /gene="nirB"
                     /locus_tag="VNZ84_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HWU66827.1"
                     /translation="MTQHTRIVVVGNGMVGHYFMQELVKRHPDLQAANLQVTVLGEEP
                     RPAYDRVHLSSFFSGSSAEDLSLVEPGFYDRPGLELRLNARVSQIDRQQHTVTLTDNT
                     ILPYDKLVLATGSVPFVPSVAGRDRDNCFVYRTIEDLEAMTAAGKTAKSGVVVGGGLL
                     GLECAKALKDMGLETHVVEFAPRLMAVQVDEAGGKVLRSKIEELGVHVHTSRNTTAIV
                     DGATARHRMVFFDETWLETDMIVFSAGIRPRDELARESGLAIGARGGIAIDEFSRSSD
                     HNIYAIGECASWNDEMFGLVAPGYDMARTACAHLFGETASTFQGADMSTKLKLMGVDV
                     ASVGDAHGKTHGCRSYQYTDEVRNIYKKIVVSEDGKKLLGAVLIGNADEYGTLQQMTV
                     NAMALPENPEFLILPASDGKEKPGLGVDALPDTAVLCSCNNVSKGAIGEAVAAGHCSI
                     GAIKSCTKAGTACGGCVPLVTQVMKAQMVKLGMTVNNHLCEHFPYSRQELYHLVRVGQ
                     IKTFGELLEKHGKGLGCDICKPTAASILASCWNDFILKKDLASLQDSNDYFLGNIQKD
                     GSYSVVPRMPGGEVTPDGLIAIGQIAKKYNLYTKVTGGARVDMFGARVEQLPNIWEEL
                     IAAGFESGHAYGKSLRTVKSCVGSTWCRYGVDDSVGLAVQLENRYKGLRAPHKIKFGV
                     SGCTRECAEAQGKDVGLIATESGWNLYVCGNGGMKPRHAELIASDLSKEDAIRMIDRF
                     LMFYIRTADRLQRTSTWRDNLEGGLEYLKDVVIHDSLGLGAELEAQMQHVVDNYQDEW
                     KTAVSDPEVRKRFRSFVNSVAGDNNIVFVKERDQMRPATVEEKQAQAKQPAKIIPILA
                     PTE"
     gene            2890..3270
                     /gene="nirD"
                     /locus_tag="VNZ84_07440"
     CDS             2890..3270
                     /gene="nirD"
                     /locus_tag="VNZ84_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HWU66828.1"
                     /translation="MSQLQQTTSSMEAIQPESLTGIWHPVCQLEDIWPNMGVCALVKD
                     QQIAIFRMQDDQLYAIDNHDPHSDANVLSRGIVGDLSGEHVVASPIYKHHYQLRTGVC
                     VEDHSVRLRTYVVELRDGAVWVQV"
     gene            3245..7453
                     /locus_tag="VNZ84_07445"
     CDS             3245..7453
                     /locus_tag="VNZ84_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nitrate reductase/sulfite reductase
                     flavoprotein subunit alpha"
                     /protein_id="HWU66829.1"
                     /translation="MAQSGYRFKQDQVEAMATQVKSVCPYCGVGCGIVMEVENQKIIK
                     VYGDKQHPTNFGRLCTKGNTCIQALADSGRLEQAYVRQQRGQEPVRTGMDAAIKETAR
                     RLRAILDEHGPDAIALYVSGQMSIESQYLANKLVKGYLGSNNIESNSRLCMASAGSGY
                     KLSLGADGPPGSYQDFDQADVFFVIGANMADCHPILFLRMMDRVKAGAKLIVVDPRRN
                     ATADKANLFMQIKPGTDLALLNGLLHLLVKNGHTDDQFIAEFTEGWEAMPAFLEDYPP
                     GKVAEITGLNEQDIRQAAEWIGTSKNWMSCWTMGLNQSTHGTWSTNAICNLHLATGAI
                     CRPGSGPFSLTGQPNAMGGREMGYMGPGLPGQRSVLVEQDRQFIEQLWNVPEGTLHTR
                     LGGGTVAMFEQMIAGTVKACWIICTNPIASMANRNNVIAALEKAELVIAQDAFLDTET
                     NRYADILLPGALWAEAEGVMINSERNMTLMQQAAHPPGEAMADWQVIAKVACEMGFSE
                     GFSYQSAAEVFAEITQASNLKTGYDIRGVSHDKLRETPLAWPCPPESEADRHPIRYLN
                     DGFSQTLKVNEDGQAPRLAFATASGKAMFFARPHMDPQELPDEGFPFVLNTGRLQHQW
                     HTLTKTGKIATLNKLNPGPFIELHPEDAAVLGIQDKDQVEISSRRGSAVLPAIVTDRV
                     RAGNCFAPFHWNDVYGEHLAINAVTIDAIDPISQQPELKFCAVSLTRVANAPPLSHEE
                     KEIAAVTEVPDTDRNKELAMKDIDALARLLGLDTAPEIMLDAQEQLYLQGFITGLRSE
                     ESRKMGGVPMLPKGAPLAPAKHLLVNGILAGIFGRSWLPNGGQLPVGMPDFSAKPASA
                     TSTVAILWASQTGNAESFAEKCAQRIKASGLQAAVFTMDSVNMAELAKHSQVLLLAST
                     FGDGDPPDNGSQFWTRLQADNAPRLENTRFTVLAFGDSNYDQFCGFGRKLDAKLEALG
                     AKRLLARAECEPDFEETANAWLDQAVTALATMPVTVTDPEMAIASEIVNVPGIYTETR
                     SAPVYTRLSPLRTKLVINQRLNADGAEKEIRQFGFDLTGSGFQYEAGDALGVWPSNCP
                     ELVGEMLRALKLSASTMVTVKDYGELALGDALLHHYEIARITPELLKFVQQRSASPTL
                     ALLMEDEQRLKDWLWGKQIIDLLHDFPIKVNATDWLAALKRLQPRLYSISSSPKVYPD
                     QVHLTVSTVRYGEGMSRGGVCTTFMADRAKDSEVAIFLQRSAHFRPPKNMDAPLIMVG
                     PGTGIAPFIAFLQERQASGAQGKNWLFFGEQHASSDFYYREDLEALQKVNVLTRLDTA
                     FSRDQAEKIYVQHRMLEQGEELWHWLQQGAHFCVCGDASRMAKDVDAALKTIIQTHGN
                     MPADAAADYVARMAQEKRYVRDVY"
     gene            complement(7464..8339)
                     /locus_tag="VNZ84_07450"
     CDS             complement(7464..8339)
                     /locus_tag="VNZ84_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWU66830.1"
                     /translation="MHNTKVYLMATLAAVFWGANFNLASHVLAEMTPYVAGASRYALA
                     ACVMLLLVFARRWSVPLKHWRAYLTLGVVGVFGFNLFFFLGMETSSAINGALIMALNP
                     LLTAVLGYLILGVRPSNQQLLAFPVGIVGVAIVVLGAGAQFTLARGDLFILIAALNWA
                     LYNVLVKKMMPAEVSGIASTAGIMTFGAIALVLAAILHGDAFVMPGLHAGVALMIMTL
                     GGGVLAYLFWNASIAHLGPSKAAIFLNLVPVTAMVIAAFEHMPPNHAQLFGAVLVISA
                     VTFSSVSFTNARQSV"
     gene            complement(8399..8710)
                     /locus_tag="VNZ84_07455"
     CDS             complement(8399..8710)
                     /locus_tag="VNZ84_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU66831.1"
                     /translation="MRQIKHPSIDQIELTDIMYALSDPTRLEIVARLANAGRKMTCGE
                     FDLNRPKSSMSHHFKILRAAGLVETLIEGTEHMNALRVNEIEQKFPGVLQSVLQALHR
                     S"
     gene            8794..9984
                     /locus_tag="VNZ84_07460"
     CDS             8794..9984
                     /locus_tag="VNZ84_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU66832.1"
                     /translation="MKKEMSSSGIPLALYALMAGAFGIGTTEFVIMGLLPEVSRDLQV
                     SIPMAGLLISGYALGVAVGAPIMSLLMARFSRKSSLLWLMGIFTLGNLICAIAPDYTT
                     LMVARVITSFAHGTFFGIGAIVATGLVPKDKRAFALALMFTGLTVANVVGVPAGTWLG
                     QMLGWRSTFWAVTMIGPIALAALYYLLPKDHHTEKVDVKSEINTLLQPQVIAGLLLTI
                     IVDTGLFAILTYVAPILTDVAHFSPAAVSPVFLLFGAGMVIGNILGAKLADIHLDKTL
                     IGALTFIMIILASFSLFAHQQIAVVVLVGLLGIAGFAAVPSLQIKILEKASGAPTLAS
                     AMNIGAFNIGNAAGAWVGGLVITHGLGLTAIGWIAACIVFTGLVLTVIEMQLLTNRTP
                     SNPA"
     gene            10001..11107
                     /locus_tag="VNZ84_07465"
     CDS             10001..11107
                     /locus_tag="VNZ84_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HWU66833.1"
                     /translation="MTDLFSPYQLKDITLRNRIAIPPMCQYMAEEGVANDWHLAHYTS
                     LARGGSGLVIVEATGVAPEGRITPGCLGIWNDAQAEQLREIAAAIKAAGAVPGIQIAH
                     AGRKASANKPWEGDDHIPENDPRAWQPIGPSAIAFGGDLPRVPKEMSIDDIQRIQQDF
                     VAAAKRALDAGFEWLEIHFAHGYLGQSFTSAHANQRTDIYGGSVENRSRFTIETLEAI
                     RKVWPENLPLTARFGVIEYDGRDEETLSESIALVKQWKQRGLDMLSVSVGFTIPDTNI
                     AWGPAFLGPIAQRVRNEAGIPVSSAWGFGAPALADNAIRNEQLDLVMIGRAHLENPHW
                     PYFAAKALHQENAAWVLPAPYAHWLARYRSPTQD"
     gene            complement(11204..11818)
                     /locus_tag="VNZ84_07470"
     CDS             complement(11204..11818)
                     /locus_tag="VNZ84_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU66834.1"
                     /translation="MTTETKKARGRPRVFDPDDALDKALQIFWNRGYEGTSLAELTET
                     LGVNKPSLYAAFGNKEELFMKALSKYATGPVAFVKDVINEPTARAVAQRFLYEAADFL
                     TQTSHPKGCMIVQGALSGGESAEMVKQVLIKYRAGYENFLTKRFEQAKQDGDLPRDAN
                     PQALAKYLATLHQGMSVQATSGASKEDLLAIADLALSAWPKASA"
BASE COUNT         2889 a         3168 c         3109 g         2677 t
ORIGIN      
        1 ttatctcacc gccttggttt taaaacatag gcaaaccttt tttggcatat caattgctta
       61 tccagtggta aatctcactc acagacgatg agtttttaat cagggagttt ccgctaccag
      121 gaacatccct atatggacaa aggcgtccat agcttattta cgaactaccc taccagtagt
      181 cgccagataa gctatggacg cctttttgcg tttttaagct ttgtaaatcg caatgcatgc
      241 acatggaaca acgaaggcca tgactgtacg cttgcctcag atggggaaca caagatgact
      301 caacacaccc gcatagtagt agtcggtaac ggcatggtgg gacattattt tatgcaggag
      361 ctggtaaaac gccacccgga cctgcaagcc gcaaacctgc aagtcacggt attgggtgaa
      421 gaacctcgcc cggcctatga ccgcgtgcat ctttccagct ttttctctgg cagcagcgcg
      481 gaagacttgt cactggtaga gcctggcttt tatgaccgcc ctggcctgga gctacgcctg
      541 aatgcgcgcg tcagccagat tgatcgtcaa caacatactg tcaccctgac agacaacact
      601 atcctgcctt acgacaagct ggtattagcc actggctcgg tgccattcgt gccatccgtc
      661 gctggccgtg accgcgacaa ctgctttgtc tatcgcacga ttgaagacct ggaagccatg
      721 acggctgctg gtaaaactgc caagagtggt gtcgttgtgg gtggtggact gctgggcctg
      781 gaatgtgcca aagcattaaa agacatgggt ctggaaaccc atgtcgttga atttgcaccg
      841 cgcctgatgg cagtacaagt tgatgaagcc ggcggtaagg tattgcgcag caaaatcgaa
      901 gaactgggtg tacacgtgca caccagccgc aataccaccg cgattgtaga cggcgcgact
      961 gcacgccacc gcatggtgtt ttttgatgaa acctggctgg aaaccgacat gatcgtgttt
     1021 tccgccggca tccgtccacg tgacgagcta gcacgcgaat ctggcctagc catcggcgca
     1081 cgtggtggta tcgccattga tgagttctcc cgctccagcg atcacaatat ttatgccatc
     1141 ggcgaatgtg ccagctggaa cgacgaaatg ttcggcctgg tggcccctgg ttacgacatg
     1201 gcccgcacag cctgtgctca tttgtttggt gaaactgcct ccactttcca gggggcagac
     1261 atgagcacca aactcaaatt gatgggcgtg gatgtcgcca gtgtcggtga cgcgcatggc
     1321 aaaacccacg gttgccgcag ttatcagtac accgatgagg tgcgcaatat ctacaaaaaa
     1381 attgtagtca gcgaagatgg caaaaaactg ctgggcgcag tgctgattgg caatgccgat
     1441 gaatatggca ctttgcagca gatgacagtc aatgccatgg ccttgccgga aaatcctgag
     1501 ttcctgattt tgcctgcaag cgacggtaaa gaaaagccag gtttgggcgt ggatgcacta
     1561 cctgatactg ctgtgctgtg ttcatgcaat aacgtcagca aaggcgccat cggcgaagcg
     1621 gtggcagccg gtcattgcag cataggtgcg atcaaatcct gtaccaaagc cggcacggct
     1681 tgcggcggct gcgtgccatt agtgacacaa gtcatgaaag cgcagatggt caagctcggt
     1741 atgaccgtca acaatcacct gtgtgaacac ttcccctact cccgccagga gctgtaccac
     1801 ctggtgcgcg taggacaaat caagactttt ggcgaactgt tggaaaaaca tggcaaaggc
     1861 ctgggctgcg acatctgcaa accaaccgct gccagcatcc tggcttcttg ctggaatgac
     1921 tttatcctga agaaagatct ggcctcactg caagactcca acgattactt cctgggcaat
     1981 atccaaaagg atggcagtta ttccgtcgtt ccacgtatgc cgggtggtga agtcacgccg
     2041 gatggcctga tcgccatcgg ccagatcgcc aagaaataca atttgtatac caaagtcact
     2101 ggcggtgccc gcgtcgatat gtttggtgca cgcgtcgagc aattacctaa catctgggaa
     2161 gaactcatcg ctgcagggtt tgaatccggc catgcctacg gcaaatcgct gcgtaccgtg
     2221 aaaagctgtg tcggctcaac ctggtgccgc tatggcgtag acgatagcgt cggcctggct
     2281 gtgcagttag agaaccgtta caagggcttg cgtgcaccgc acaaaatcaa gtttggtgtg
     2341 tcaggttgta cgcgtgagtg tgcagaagca caaggtaagg acgtcggttt aattgccacc
     2401 gaaagcggct ggaacttgta tgtctgcggt aacggcggca tgaagccacg tcacgccgaa
     2461 ctgatcgcga gcgacctcag caaggaagac gccatccgca tgatagaccg cttcctgatg
     2521 ttctatatcc gtacggccga ccgcctgcaa cgcaccagca cctggcgcga caatctcgaa
     2581 ggcggcctgg agtatttgaa agacgtcgtc atccatgact ccttaggctt gggcgcagag
     2641 ctggaagccc agatgcaaca tgtggtggac aactaccagg acgaatggaa aaccgcggtg
     2701 agcgacccgg aagtgcgcaa acgtttccgc agcttcgtca acagcgtggc gggcgacaac
     2761 aacatcgtct tcgttaagga gcgcgaccag atgcgcccag ccacggtaga agaaaaacag
     2821 gcgcaggcaa aacagccagc gaagatcatt cctatcctcg cgcctactga gtaatccacg
     2881 gagtcaaaca tgagtcagtt acaacaaacc acttcctcga tggaggctat ccagcctgaa
     2941 tccctgaccg ggatctggca cccggtgtgt cagctggaag atatctggcc aaatatgggc
     3001 gtttgcgcgc tggtcaaaga ccagcagatc gccattttcc gcatgcagga tgaccagctg
     3061 tatgccatcg acaaccatga tccacatagt gatgccaatg tgttatcgcg tggcatcgtc
     3121 ggtgacctga gcggcgaaca cgtggtggcc tcccccatct acaaacatca ttaccagttg
     3181 cgtacgggtg tttgcgtgga agatcacagt gtccgcctgc gcacctatgt ggtggaactg
     3241 cgtgatggcg cagtctgggt acaggtttaa acaggatcag gtagaagcaa tggcgacaca
     3301 ggttaaatca gtctgcccct attgcggtgt gggttgcggc attgtcatgg aagtcgaaaa
     3361 ccagaagatc atcaaggttt acggcgacaa acagcacccc accaattttg gccgcctgtg
     3421 taccaaaggg aacacctgta tacaagccct tgccgactct ggccgcctgg aacaggctta
     3481 tgtcaggcag caacgcggac aagagcctgt gcgtactggc atggacgccg ccatcaagga
     3541 aaccgcacgc cgtttacgcg ctattctgga tgaacatggc ccggatgcca tcgcactcta
     3601 tgtctccgga cagatgtcga ttgagtcaca atacctggcc aataaactgg tcaaaggtta
     3661 tctcggcagt aacaacattg aatccaactc ccgcctgtgc atggccagtg ccggcagcgg
     3721 ctacaaactc tcgttgggtg cggacggccc acctggctct taccaggatt ttgaccaggc
     3781 cgatgtgttc ttcgtcatcg gcgccaacat ggccgattgc caccctatcc tgttcctgcg
     3841 catgatggat agggtcaagg ctggtgccaa gctgattgtg gtggatccgc gccgcaatgc
     3901 cactgccgac aaggcgaacc tatttatgca gatcaagccc ggcaccgacc tggccttgct
     3961 caacggcctg ttgcatctgc tggtgaaaaa cggtcatacc gatgaccagt ttatcgccga
     4021 atttaccgaa ggctgggaag ccatgccggc atttctggaa gactacccgc ctgggaaagt
     4081 tgcagaaatc accgggctca acgagcaaga tattcgccag gcggctgagt ggataggcac
     4141 cagcaaaaac tggatgagct gctggaccat gggcttgaac cagagcacac atggcacctg
     4201 gagtaccaat gcgatttgta acctgcatct agccacaggc gccatttgcc gaccaggcag
     4261 tggtccattt tcattgactg ggcaaccgaa tgccatgggt ggccgcgaaa tgggctatat
     4321 ggggccaggc ctgccaggcc aacgctcagt gctggtcgag caagacaggc aatttataga
     4381 acagctctgg aacgtacccg aaggtacttt acatacccgc ttgggtggtg gaacggtcgc
     4441 catgttcgag caaatgatag ccggaaccgt gaaggcttgc tggatcatct gcaccaaccc
     4501 gatcgccagt atggccaatc gtaacaatgt gattgccgcc ttggaaaaag cggaactggt
     4561 cattgcccaa gacgcctttt tagacactga aaccaaccgc tatgccgata ttctgttgcc
     4621 aggggcatta tgggccgaag ccgaaggcgt catgatcaac tcagaacgca atatgaccct
     4681 gatgcagcag gcagctcatc caccgggtga agccatggct gactggcagg ttattgccaa
     4741 agtcgcttgt gaaatgggtt ttagtgaagg ctttagttac caatcagccg ctgaagtgtt
     4801 tgcggaaatc acacaggcgt ccaacctgaa aaccggttac gacatccgcg gggtgagtca
     4861 tgacaagttg cgcgaaacgc cgttggcatg gccatgcccc ccagagagtg aagcggaccg
     4921 gcatccaatt cgttacctca atgacgggtt cagccagaca ttaaaagtca acgaagatgg
     4981 tcaagcgccc agactcgcct ttgctactgc cagtggtaaa gcgatgtttt ttgcccggcc
     5041 acatatggat ccgcaggaat tacctgacga aggttttcca tttgtactta acactggccg
     5101 cttgcaacat caatggcata cgctgaccaa aaccggcaag attgccacct tgaacaaact
     5161 gaatccggga ccgttcatcg agctgcaccc tgaagatgct gctgtactcg gcattcagga
     5221 taaagaccag gttgaaatta gctcccgccg tggcagcgct gtgctacccg ccatcgtcac
     5281 ggatagagtc cgcgcaggca actgttttgc gccctttcac tggaatgatg tctatggtga
     5341 acatctggcc atcaatgcag tgaccatcga tgccatcgac cccatttcgc agcaaccaga
     5401 attaaagttt tgtgctgtgt ccctgacgcg cgttgccaac gcgcctcccc tatcccatga
     5461 agaaaaagag attgctgcag tcactgaagt ccctgacaca gatcgcaata aggaactcgc
     5521 tatgaaagac attgatgccc tggccagatt gctggggctg gatacggcac cggagatcat
     5581 gcttgatgcg caggaacagc tgtacttgca aggctttatt accggtttgc gctcagagga
     5641 atcacgcaaa atgggtggcg tacccatgtt acccaaaggc gcgccgcttg cgccggccaa
     5701 gcacttgcta gtcaatggca tattggctgg aatattcggt cgcagctggt tacccaacgg
     5761 cggccaatta cccgtcggga tgccagactt ttctgccaaa cctgcgtccg ccacgtccac
     5821 cgtcgcgatt ctgtgggcct cacaaaccgg taatgctgaa agctttgccg agaaatgtgc
     5881 acagcgcatc aaagccagtg gcttacaggc cgctgtgttc accatggaca gtgtcaatat
     5941 ggctgagctg gcaaaacact cccaagtgtt actactcgcc agtacgtttg gtgatggtga
     6001 cccgccagat aatggcagcc agttttggac ccgcctgcaa gcagacaatg caccaagact
     6061 ggaaaatacc cgttttacgg tcctggcatt cggtgactcc aactatgacc agttctgcgg
     6121 ttttggccgc aagctggacg ccaaactgga agcgcttggt gccaagcgtt tactggcgcg
     6181 tgcggagtgc gagccagact ttgaagaaac cgcgaatgcc tggctggatc aagccgtgac
     6241 agcattggca accatgcctg tgactgtcac tgatccagag atggcaatag caagtgagat
     6301 tgtgaatgtg ccaggcattt ataccgaaac aaggtcagcg cccgtgtata cccggctatc
     6361 ccctttacgc acaaaactgg tgattaacca acgcctgaat gctgatggtg ccgaaaagga
     6421 aatccgccag tttggttttg atttaaccgg tagcggcttc caatatgagg caggcgatgc
     6481 gctgggtgtt tggccgagca actgtccgga actggttggc gaaatgctgc gtgcattgaa
     6541 gctctcggcc tctacgatgg tcaccgtcaa agattatggt gaactggcac tgggtgacgc
     6601 gctgttacat cactatgaaa tcgcacgcat caccccggag ctgttgaagt ttgtgcagca
     6661 acgcagtgcc agcccgacac tggcattact catggaagat gagcagcggc taaaagactg
     6721 gttatggggc aaacagatca tagatctgtt gcatgatttc ccgatcaagg tcaatgcgac
     6781 tgactggttg gctgcattaa aacgcctgca gccacgcctg tattccattt catccagtcc
     6841 caaggtttat cctgaccagg tacatttaac cgtatccacg gtacgctatg gcgaaggcat
     6901 gtcacgtggt ggcgtctgca ccacctttat ggctgaccgg gcgaaagact cagaggtagc
     6961 cattttcctg caacgctccg cacacttccg tccgccaaaa aatatggatg cgccgctgat
     7021 catggtcggc cccggcacag gcatcgcacc gtttatcgcc ttcttgcaag aacgccaggc
     7081 cagcggcgca caaggcaaaa actggctgtt ttttggtgaa caacatgcgt cgtcagattt
     7141 ttactatcgt gaagacttgg aagcactgca aaaagtcaat gtgctcacca ggctggatac
     7201 ggcattctca cgcgatcagg cagaaaaaat ttacgtacag caccgcatgc tggaacaagg
     7261 cgaggaacta tggcactggc tgcaacaagg cgcgcacttc tgcgtctgtg gcgatgccag
     7321 ccgcatggcc aaagatgtgg atgccgcact gaaaacgatt attcaaacac atggcaatat
     7381 gcctgcggac gccgcggctg actatgtcgc caggatggcg caagaaaagc gctatgtgcg
     7441 ggatgtctac taagtctccg aggctacact gattgacgcg cgttggtgaa ggaaaccgaa
     7501 ctgaaagtca ccgcgctgat caccagcacc gcgccaaata gttgcgcatg attgggcggc
     7561 atatgttcaa acgcggcaat caccattgct gtcacgggga ccagattaag gaaaatcgcc
     7621 gctttcgatg ggccgagatg agcgatgctg gcgttccaaa acaaatacgc cagaacgccg
     7681 ccacccaaag tcatgatcat caaggcaact cccgcgtgca aaccaggcat gacaaaggca
     7741 tcgccatgca gtatcgccgc cagcaccagc gcaatggcgc caaatgtcat gataccggcc
     7801 gtgctggcaa tgccactcac ctcggcaggc atcattttct tcaccaagac gttatacaac
     7861 gcccagttca acgcagcgat cagaataaac aggtcacccc gcgccagtgt gaactgcgca
     7921 ccggcgccca gcaccacaat ggccacgccg acaatcccaa ccgggaaggc caacaactgc
     7981 tgattactgg gccgcacccc taatatcaga taaccaagca cggccgtcag taagggattc
     8041 agcgccataa tcaatgcacc gttgatcgca gaagaggttt ccatacccag gaagaaaaac
     8101 aggttaaatc caaatacgcc taccactccc agcgtgaggt aggcacgcca gtgttttagc
     8161 gggactgacc agcggcgggc aaacaccagc aacaacatta cgcaggctgc cagcgcatag
     8221 cggctcgcac cggccacata cggcgtcatt tcagccagca catggctggc caagttgaaa
     8281 ttagcgcccc agaacacggc tgccaacgtg gccattaaat aaacttttgt attatgcata
     8341 agccttttat atagaaaaag gaaggttgtg acttgtgtgg cggatataca gccgttgttt
     8401 aagaacgatg cagcgcctgc agtaccgatt gcagcacgcc gggaaacttc tgctcaatct
     8461 cgttcaccct gagcgcattc atatgctcgg tcccttcaat caaagtttca accagcccgg
     8521 cagccctgag gattttaaag tggtgtgaca tgctggactt ggggcgattg aggtcaaact
     8581 cgccacaggt catcttgcgc ccggcatttg ccaggcgtgc cacaatttcc aggcgcgtag
     8641 ggtccgacaa ggcatacatg atgtccgtca actctatctg gtcaatactg ggatgtttga
     8701 tttgtcgcat ggttaaaatt tagcacagcg cttgatatta ttccatagtt cgattatatt
     8761 cgaactatgg aatttaatcg agtgtaatca gtcatgaaaa aagaaatgtc gtcatcaggc
     8821 attccgcttg ccttgtatgc cttgatggca ggtgcctttg gcataggtac cacagagttt
     8881 gtgattatgg gattgttacc tgaagtcagc cgcgacctgc aggtcagtat tcccatggcc
     8941 ggattattga ttagcggtta tgcgctgggg gttgccgtgg gcgcacccat catgtcactc
     9001 ctgatggcca gattttcccg taaaagctcc ctgctctggc taatgggtat ttttacctta
     9061 ggtaacctga tctgcgcaat cgcccctgat tacaccacat taatggtcgc cagggtaatc
     9121 acctcattcg cgcatggcac tttctttggg ataggcgcca ttgtagccac tggcctcgta
     9181 cctaaagaca aacgcgcgtt tgcactggca ttgatgttta ccggcctgac ggtggctaat
     9241 gtagtggggg ttcctgccgg gacctggctg ggacagatgt tgggctggcg gtctaccttc
     9301 tgggcggtga ccatgattgg gccgatagcg ctggcagcct tatattacct gctgcctaaa
     9361 gatcaccaca ccgaaaaagt agatgtgaag tctgaaatca acactttgct gcaaccgcag
     9421 gtcattgccg gattgttatt gaccattatt gtcgataccg gtttatttgc catcttgacc
     9481 tatgtcgccc ctatcctgac ggatgtagca catttttctc cagcggcagt ctcgccggtg
     9541 ttcctgctat ttggtgcagg catggtgatc ggcaatattc tcggtgcaaa actcgcggat
     9601 attcaccttg ataaaacgct gatcggcgcc ttgacgttca tcatgatcat cctggcttct
     9661 ttttccttgt ttgcccatca gcaaattgcc gtcgtcgtgt tagtcggcct actcggcatt
     9721 gccgggtttg ccgcagtccc ttcattacag atcaagattc tggaaaaagc cagtggtgca
     9781 ccaacactgg cctctgccat gaacattggc gccttcaata tcggtaatgc agcgggtgcc
     9841 tgggttggtg ggctagtgat tacgcatggc ctgggcctga ctgcgattgg ctggattgcc
     9901 gcctgtatcg tatttaccgg actggtactc accgtgattg aaatgcaact gcttaccaat
     9961 agaacaccat caaatcctgc ttaaacttta aaggactccc atgactgatt tattttcacc
    10021 ttatcaatta aaagacatca ccctcagaaa ccgtatcgcg atcccgccga tgtgccagta
    10081 tatggccgaa gaaggtgttg ctaacgactg gcacctggcc cattacacca gcctggcacg
    10141 tggtggtagc ggcctggtga ttgttgaagc aaccggcgtg gcgccagaag gacgcatcac
    10201 accagggtgc ctgggcatct ggaatgatgc acaagcagaa caactacgtg aaattgctgc
    10261 tgccatcaag gcagcaggtg cggtcccagg tatccagatc gcgcatgcag gccgcaaagc
    10321 cagcgcaaat aaaccgtggg aaggtgatga ccatattcca gaaaacgacc cgcgcgcctg
    10381 gcaaccaatc ggcccttcag cgattgcctt tggtggcgac ctgccccgcg tccccaaaga
    10441 aatgtcgata gatgatatcc agcgcatcca gcaggacttt gttgccgccg ccaaacgggc
    10501 actggatgcc gggtttgaat ggctggaaat ccattttgca catggctacc tggggcaaag
    10561 ttttacctct gcacacgcta accaacgtac cgacatctat ggtggcagtg ttgaaaaccg
    10621 ttcgcggttt accatagaaa ccctggaagc catccgtaag gtctggccgg agaatctgcc
    10681 tctcaccgcc cggtttggcg tgattgaata cgatggcaga gatgaagaaa ccttgagcga
    10741 atccattgca ttggttaaac aatggaaaca gcgtggcctg gatatgttga gcgtcagcgt
    10801 cggctttacc atcccggaca ccaatattgc ctggggacct gctttcctgg ggccaattgc
    10861 acaacgtgtg cgtaacgaag ccggtatccc ggtctcttct gcctggggct ttggtgcgcc
    10921 tgcgctggca gacaatgcca tccgcaacga acagctggat ctggtcatga taggccgcgc
    10981 ccatctggaa aacccgcact ggccttactt tgcagccaaa gcgttgcatc aggaaaatgc
    11041 cgcctgggtg ttacctgcgc cctatgcgca ctggttggcg agatatagaa gcccaacgca
    11101 agactagtga tattgctgcg ttcaacataa aacgagggac aggagctatt tctgtccctt
    11161 tctttttttg ctgatatgcg taaggcttgg cctgctttta tattcaggcc gaagccttgg
    11221 gccaggcact tagtgccaga tcagcaatcg ccaataaatc ctcttttgaa gcgccgctgg
    11281 ttgcttgcac cgacataccc tgatgcaaag tggcaagata cttggccaat gcttgtggat
    11341 tggcatctct gggcaaatcg ccatcctgtt tagcctgctc aaagcgtttg gttaaaaagt
    11401 tttcataacc cgctctatat ttaatcagca cttgtttaac catctcggca ctttcgcccc
    11461 ctgacaaagc gccttgtaca atcatgcagc ctttagggtg tgaagtctgg gttaaaaaat
    11521 cggctgcttc gtataaaaaa cgttgtgcca ctgcacgcgc cgtgggttca ttgatcacat
    11581 ctttgacaaa ggccaccgga cctgttgcat atttcgatag cgccttcatg aacagctctt
    11641 ctttgttacc aaaggcggca tacaaacttg gcttgttcac ccctagagtt tccgtcagct
    11701 cagccagtga ggtgccttca tagcctctat tccagaaaat ctgcagcgct ttatcaagtg
    11761 catcatcggg atcgaagact cttgggcgcc cacgtgcttt tttagtttct gtggtcatgt
    11821 tttcaaactt tcaattaaat aat
//
LOCUS       DATMSK010000223         4454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_300003,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000223 DATMSK010000000
VERSION     DATMSK010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300003"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..2206
                     /gene="ovoA"
                     /locus_tag="VNZ84_07475"
     CDS             101..2206
                     /gene="ovoA"
                     /locus_tag="VNZ84_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HWU66835.1"
                     /translation="MSKHPHRSIRLNSSQTPLTREQIRAYFHETCDIYESLFTVLSSD
                     EAYYVKPIALRHPLIFYYGHTHTFFINKLILAGLISERVNPKFESMFAVGVDEMSWDD
                     VNEAHYQWPTPAEVKAYRARVREVVDALINTLPLDTPIDWDSPWWPILMGIEHERIHL
                     ETSSVLIRQHALSYVQPSAAWAPSSALAVAAPENTLVQVPAHEIVLNKSEDYYGWDNE
                     YGHFHTHCPAFEAAKYLTSNAEFLQFVEAGGYQNDAYWQEEGLAWRQYTQAEHPTFWI
                     KQDNGWKLRLMTEEVDMPWSWPVEVNYHEAKAFCNWLAAQTGKPVRLPTEHEWYSLYE
                     HAGLSDDNIANSNHANLFLDHGASSCPVNQFAQGELYDVVGNVWQWTETPIFPFDGFK
                     VHPLYDDFTTPTYDGRHNLIKGGSWISTGNEAIKHSRYAFRRHFFQHAGFRYIVTDTP
                     VTQKDAYYESDKQLSEYAEFHYGDSYFGVPNFSKALSDYALQHLQAKPKRSALDLGCA
                     TGRATFELAKQFDHVTGIDFSARFIGLALQAAEQGVLRYTMTNEGDLVSYQERTLSAL
                     GLDQVVDKVAFWQGDACNLKPQFTGYDFILAANLIDRLYSPRQFLSSIHERLNMGGVL
                     MIASPYTWLEEHTPREEWIGGYKQDGENVTTLDGLKALLGPHFKLMQDPVDIPFVIRE
                     TRHKFQHTLSEVTLWERVR"
     gene            2203..3414
                     /locus_tag="VNZ84_07480"
     CDS             2203..3414
                     /locus_tag="VNZ84_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HWU66836.1"
                     /translation="MTSRFDDPVAREGSGALKFDGRQQTFGTTEVMPMWVADMDFAVP
                     EAVTQALQARIAHPVLGYSLAPDSLYQALMDWLATKHDWQVPREWIVLTPGVVPSLNM
                     VVAALTKPDDGVIVQPPVYFPFFSAVTGQQRQLIENPLRLMNDASQPHYQMDMNHLAR
                     CAQQARLLLLCSPHNPVGRVWQTAEMETILSIADEHDLIVLSDEIHADLVYADAHHQP
                     LSRLAMQAETAEQTAQSHRVITAVSPSKTFNIPGLGLSALIVPNTAHRQAIQRHLNTL
                     GISVTNPLNMAAFEAAYRGGHDWLNELIPYLQATRDAAVAYIQTELPKIKVISPEATY
                     LLWLDCRALQLDDNALKRFFIEEAQLGLSPGAMFGHGGEGFMRMNIGTSKANVLAALA
                     RLKAALTLHTF"
     gene            3448..4212
                     /locus_tag="VNZ84_07485"
     CDS             3448..4212
                     /locus_tag="VNZ84_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWU66837.1"
                     /translation="MQIETKRFTRLLSKNRLCSKNVFSILMLFALCAVTLAQANTLEE
                     AKQKLAEKDYAAAHAIYLSLANQNDAKACYNLGLMYQDGDGVTKNMDEAVKWYTKSAD
                     LGYKEAQYTLASLVFQRQTQSISYPQAVTYYEQAAKQGHVKSQLNLGMLYLRGDVIAQ
                     DMPAAVQWLSLAASNNNSEAQGYLADLYQQGAGVEQNTVKAAMWLLLATQNEDKRFVN
                     RHTKMLNYLAAQMTDEQKSSAKKLAAQCKNQQLKGC"
     gene            complement(4250..>4454)
                     /locus_tag="VNZ84_07490"
     CDS             complement(4250..>4454)
                     /locus_tag="VNZ84_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66838.1"
                     /translation="DPYVICLLKNGVSVELTKILVDKKYRAYVTFDLINDRVEISQRI
                     HGILENDDVNKVLLFELSFHLVK"
BASE COUNT         1151 a         1206 c         1092 g         1005 t
ORIGIN      
        1 actggcaccg gcatagacct gagttgcgac ccgactttcc agcgggcatt cacaggctta
       61 aaaggcttaa aactaaaaac ataaacacac gataaacact atgagcaagc atccccatcg
      121 aagtatccgg ttaaacagtt ctcaaacacc gctgacccgt gagcagatcc gggcttactt
      181 tcatgaaacc tgcgatattt atgaatccct gtttaccgtc ctgagcagtg acgaggccta
      241 ttacgtcaag ccgattgccc tcaggcatcc gctgattttt tactacggcc atacccatac
      301 gttcttcatc aacaaactga tcctggccgg gctgatctcc gaacgcgtga atcccaagtt
      361 tgagtccatg tttgccgtgg gtgtggatga aatgagctgg gacgatgtta acgaagcgca
      421 ttaccaatgg ccgacgcctg ccgaagtgaa agcctaccgt gcacgtgtgc gcgaagtggt
      481 agatgcattg atcaacaccc tgccactgga tacaccgata gactgggaca gcccgtggtg
      541 gccgatttta atgggtatag agcatgagcg catccatctg gaaaccagct cggtactgat
      601 ccgccaacac gccctcagct atgtgcagcc ctcagccgcc tgggcgccct cttctgcgct
      661 ggcggtcgcc gcccctgaaa ataccctggt acaggttccc gcgcacgaga tcgtccttaa
      721 taaatcagag gattactacg gctgggataa cgaatacggt catttccaca cccactgtcc
      781 ggcattcgag gcggccaaat acctcacctc caatgccgag tttttgcaat ttgtggaagc
      841 aggtggctat cagaacgacg cctactggca agaagaaggc ctggcctggc gccaatacac
      901 acaggccgaa caccccacct tttggatcaa acaggacaat ggctggaaac tgcgcctgat
      961 gacggaagag gtcgacatgc cctggagctg gccggtagaa gtgaattacc atgaggccaa
     1021 agcattttgc aattggctgg ctgcccaaac cggcaaaccc gtgcgtctgc ccaccgagca
     1081 tgagtggtat agtctgtatg aacatgcggg cctgtcagac gacaacatcg ccaatagcaa
     1141 ccacgccaac ctgtttttag accatggcgc ctcgtcttgc ccggtcaatc agtttgcaca
     1201 gggtgagctg tatgatgtgg tgggcaatgt ctggcaatgg acagaaacgc cgatcttccc
     1261 gtttgatggg tttaaagtgc accccttata cgatgacttt accaccccga cttacgatgg
     1321 tcgccataac ctgatcaaag gcggctcgtg gatttcaacc ggtaacgagg cgattaaaca
     1381 ctcacgctac gcgtttcgcc gccacttttt tcaacatgca ggattccgct atattgtgac
     1441 tgacacgccc gtgactcaaa aagacgccta ttacgaatca gacaaacagt tgtccgagta
     1501 tgccgaattt cattatggcg acagctactt tggcgtgcct aacttctcca aagccttgag
     1561 cgattatgcc ttgcagcatt tgcaagccaa gcccaagcgc agcgcgctgg atttaggctg
     1621 tgccaccggg cgcgctacct ttgagctggc caaacagttt gatcacgtca ccggcatcga
     1681 tttttctgcc cgctttattg gcctggcact gcaagcagcc gagcaaggcg tattgcgcta
     1741 taccatgacc aacgaaggcg acctggtcag ctaccaggaa cgcacgttga gtgccttagg
     1801 cttagaccag gtggtcgaca aggtcgcatt ctggcaaggc gatgcctgta acctcaagcc
     1861 gcaattcacc ggctacgact ttattctggc agcgaattta attgaccgcc tgtatagccc
     1921 gcgtcagttt ttgagcagca ttcacgaacg tctgaatatg ggtggcgtgc ttatgatcgc
     1981 ctcgccttat acctggctgg aagaacacac gccacgtgaa gaatggatag gcggctacaa
     2041 gcaggatggc gaaaatgtca ctacgctgga tggcctcaaa gccttgctgg gcccccactt
     2101 taaactcatg caagacccgg tagacatccc atttgtcatc cgcgaaaccc gccacaaatt
     2161 ccagcatacg ctgtcagaag tgacactctg ggagcgcgtg cgttgaccag ccgctttgac
     2221 gatcccgtcg cgcgtgaagg cagcggcgca ttaaaattcg atggccgcca gcagaccttt
     2281 ggcaccactg aagtcatgcc gatgtgggta gccgatatgg attttgccgt gcccgaagca
     2341 gtgacccaag ccttgcaagc ccgaattgcg catccggtgt tgggttattc gctggcgcca
     2401 gacagccttt atcaagcact gatggattgg ctagcgacca agcatgattg gcaagtgcca
     2461 cgtgagtgga ttgtgctcac gccaggcgtg gtgccttcgc tgaatatggt ggtcgcagct
     2521 ttgactaaac cagacgatgg cgtgattgtg caaccgcccg tgtatttccc gtttttctca
     2581 gcggtcaccg ggcaacagcg tcaactcatt gaaaacccgt tacgcctgat gaatgacgca
     2641 agccagccgc actaccagat ggatatgaac cacctggcta ggtgtgccca acaggcgcga
     2701 ctgttattac tgtgttcacc gcacaacccg gtgggccgcg tgtggcaaac ggctgaaatg
     2761 gaaaccattt taagcatcgc tgatgagcac gatttaattg tgctctccga tgaaatccat
     2821 gccgatttag tctatgccga tgcgcaccac cagcctttaa gccgcctggc catgcaggct
     2881 gaaaccgccg agcaaactgc gcagtcgcac cgcgtgatta ccgccgtctc ccccagcaaa
     2941 acctttaata tccccggcct ggggctctca gctttgattg tacctaacac cgcccatcgg
     3001 caagccattc aacgccacct caatacgcta ggcatctctg tgaccaaccc gctcaatatg
     3061 gccgcgtttg aggccgctta tcgcggcggt cacgactggt taaatgagtt gatcccatat
     3121 ttgcaagcca cacgcgatgc ggcagtggcg tatatacaaa cagagttgcc gaagattaaa
     3181 gtgatctccc cagaagccac ttatttgttg tggctagatt gccgcgcact ccagctggat
     3241 gacaacgcat taaaacgctt ttttattgaa gaagcacaac tcggcctctc ccccggcgcc
     3301 atgtttggtc atggcggtga agggtttatg cgcatgaaca taggcaccag caaagccaac
     3361 gtgctagcag cgctggcgcg attaaaagca gctttaaccc tccatacttt ttaaaggtcg
     3421 ttattcacca caaaaggaac acgcatcatg cagattgaaa caaagcgctt cactcgcctg
     3481 ctctcaaaga ataggctgtg ctctaaaaat gtgttctcta tattgatgct attcgcgctg
     3541 tgtgcggtaa ctctggcgca ggcaaatact ttagaagaag ccaaacaaaa actcgccgaa
     3601 aaagactatg cggccgccca tgcgatttat ctgtcactcg ccaatcaaaa cgatgccaaa
     3661 gcctgctaca acttaggcct gatgtaccag gatggcgatg gcgtcaccaa aaacatggat
     3721 gaagcagtca agtggtacac caagtcggcc gacctgggtt acaaagaggc acagtacacg
     3781 ctggcctcac tggtgtttca gcgccagacg caaagcatca gttacccgca agccgtgacc
     3841 tactacgagc aggcagccaa gcaagggcat gtgaaatcgc aactcaattt aggcatgctg
     3901 tatttgcgcg gtgatgtgat tgcgcaagat atgcccgccg ccgtgcaatg gctaagcctg
     3961 gccgcgagca ataacaatag tgaagcgcaa ggttatctgg ccgacttata ccagcaaggg
     4021 gccggggtgg aacaaaacac cgttaaagcg gcgatgtggt tactattggc gacgcaaaat
     4081 gaagacaagc gctttgtgaa ccgccacacc aagatgctga attatcttgc tgcgcaaatg
     4141 actgatgagc aaaagtcatc tgctaaaaaa ttggcagcgc agtgtaaaaa ccaacaattg
     4201 aaagggtgct gaatacagtc ggagtaatga tcaaatgatt ggtcactctt tatttaacta
     4261 aatgaaaact taactcaaat agtaatactt tattaacatc atcattttca agaattccat
     4321 gaatccgttg gcttatttct actcggtcat taattaaatc aaaagttaca tacgctctgt
     4381 atttcttatc aactagaatt ttcgttagct cgactgagac accattctta agtaaacaaa
     4441 ttacatacgg atcc
//
LOCUS       DATMSK010000224         5448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_228592,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000224 DATMSK010000000
VERSION     DATMSK010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228592"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VNZ84_07495"
     CDS             complement(<1..585)
                     /locus_tag="VNZ84_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HWU66839.1"
                     /translation="MLGLAALAALWSFNSIADEASLKKTIEAAYPKFKVDSVTKTPYN
                     GLYEVFMGGQIIYTDEKFSFLIAEGRLVDPKTKKDLTGERMDELTKIDFNSLPLDQAI
                     KVVKGNGSRKLVVFSDVDCPFCKRLEQKELSNINDVTIYTFLYPIEQLHPDAANKSRS
                     IWCATNRVKAWQDWILNNKLPTTTAKCEVPLEKVG"
     gene            complement(662..1870)
                     /locus_tag="VNZ84_07500"
     CDS             complement(662..1870)
                     /locus_tag="VNZ84_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWU66840.1"
                     /translation="MAVNPPLVKTDVVIVGAGLVGLTAAIALSKLGKKIVLTDAKPAQ
                     SLAENWADDVNHWDSRIYALTNQTVDWLRSLGIWSHMPASRINPIAAMHLWSPTHSHA
                     TPNLRLDADEAHLSEMGFIVESQALINACWQVLRHAEVTVITDAPAQALQYSGHVARL
                     SLPQHEVKAGLLIGADGGRSWVREQCGVHVHQVDFAQTALVTNYRAERPHDNIARQWF
                     GTHETLALLPMPQQQVSLVWALPHADAAQKQALAAEALAAEVGARCGHVLGQLTPSGD
                     ILAFPLVQNTADTMALPHVLLLGDAAHQVHPMAGQGVNLGFQDVQALCQHIEQLPAIR
                     PLGDANFLRHVMRTRQMDILKMHALTRGLDGLFAQPQAMWTHAAVLGLRAVENSAMLK
                     RFLIRTATQH"
     gene            complement(1860..3029)
                     /locus_tag="VNZ84_07505"
     CDS             complement(1860..3029)
                     /locus_tag="VNZ84_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWU66841.1"
                     /translation="MKTLSHPVVIGGGPVGLVLALSLQQSSVPVTVLEAQSAGAMYGD
                     GRALALSYGTRQVLEQLGVWALLETAVTAIETIHVSQQKGFGRTVLKANEHALPALGY
                     VVSYGALMQALDARIEQSAAGINIQYQAPVTGLDVHAGAADIQYSLADGSVQQLSTPI
                     AVIADGGRSLEAIPGLVRETKHYGHDALVGLVQTEQPHHNIAYERFTPHGPMALLPNG
                     KQFSLVWTGEQTYIDDIAALEDAAFLEALHAAFGDRVGKFLHIGKRFKFPLKLSKLGQ
                     TFAAHCVVIGNAAQTMHPVAGQGFNVGIRDAVALAKNVASTQAGEWGAVHRLTEYHQS
                     RQRDTGRGLQFTDFLVNVFSNDLAGVGLLRGAGLGALSMLPMARRFLVNKMSYGS"
     gene            complement(3071..4369)
                     /locus_tag="VNZ84_07510"
     CDS             complement(3071..4369)
                     /locus_tag="VNZ84_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HWU66842.1"
                     /translation="MQEFSRRRLQLMTSMQAGIAVIPTAPEVTRNRDSHYPYRFDSYF
                     YYLTGFKEPEAVLVLIAGAQPRSILFCRDKDIEREIWDGFRYGPEAAKSEFGFDEAYS
                     ISQLDEQLPKLMANQPELFFSLGADAGWDTKISGWLNAVKAQARTGVQAPGKLVDVRQ
                     ALDAMRLLKSPDEIDLMRRSAHIAAAAHNRAMQAVIPGKWEYEIEAEFLHEFYRQGAQ
                     APAYTSIVAGGANACTLHYNANNAELKAGDLLLIDAGCELDGYASDITRTFPVSGRFS
                     GPQKDLYELVLAAQAAAIASVSPQHHWNAPHEAALEVLVDGFIHYGLCQGSREAVLET
                     GSYRQFYMHRTGHWLGLDVHDAGEYKTPAGDWVMLQPGNVLTVEPGCYVRPAENVPEH
                     FWNIGIRIEDDVLVTSTGHEVITSAAIKTVAEIEALMRQS"
     gene            complement(4475..5248)
                     /locus_tag="VNZ84_07515"
     CDS             complement(4475..5248)
                     /locus_tag="VNZ84_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2927 domain-containing protein"
                     /protein_id="HWU66843.1"
                     /translation="MIDRCLWFCVGLLTIHAAMAEEAWQSSEYLQQAFFEVALHNEYA
                     VGEQPVRKWLQPIRVWLDHHTDQAEQHALLAKYHMQHLAKITGHPIGFAKSRQEANFI
                     VVFSHLQLWRNDIALVSGNLSIKPPADAACMFGVDLDDKKAIKKAWVVIPVDFAQEHR
                     QLLSCIVEETTQAMGLPNDSEKVYPSIFNDKTPESLLTGLDALLLKMLYAPAVKPGMR
                     AQQVRPVLDKLLKQWQSDGTIQDAERNVRQSELYEMMGF"
     gene            complement(5241..5447)
                     /locus_tag="VNZ84_07520"
     CDS             complement(5241..5447)
                     /locus_tag="VNZ84_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HWU66844.1"
                     /translation="MVTLKMPYGKYQGRLIADLPGHYLHWFAREGFPPGELGRLLHLM
                     REIDHNGLSSLLTPLRAEAGKLND"
BASE COUNT         1328 a         1555 c         1271 g         1294 t
ORIGIN      
        1 accgactttt tccagaggca cttcacactt ggctgtggtg gttggtaatt tattgttcaa
       61 aatccagtcc tgccaggctt tgacacgatt tgtcgcacac cagatactgc gtgatttatt
      121 cgctgcatcc ggatgcaact gctcaatcgg atagagaaag gtgtaaatcg tgacatcatt
      181 gatattggac agctcttttt gctccaggcg cttacagaat gggcagtcca catcagagaa
      241 caccaccagc ttacgactgc cattgccttt gaccacttta atcgcctggt ctaaaggtaa
      301 actattgaag tcaattttag tgagttcatc catgcgctca cctgtcagat ctttcttggt
      361 tttcgggtca accagacgac cttcggcaat caggaagctg aatttttcat cagtataaat
      421 aatctgaccg cccataaaca cttcgtaaag gccgttatat ggggttttgg tgacgctatc
      481 caccttgaat tttggatagg cggcttcaat cgtttttttc aggctggctt catcagcaat
      541 gctgttaaaa gaccacaatg ccgccaaggc agctaaaccc agcatgacag actgaccaaa
      601 aaattttctc atcactcact ctttctcaaa tatgcatcgc caggtactca tggagcacca
      661 attaatgttg ggtggctgtg cgaattaaaa aacgtttcag catagcactg ttttcaaccg
      721 ctcgtaagcc caatacggcg gcatgtgtcc acatggcttg tggctgtgca aacaggccat
      781 ccagaccacg tgtcagtgca tgcatcttca aaatatccat ctgtcgcgtg cgcatcacgt
      841 gccgcaaaaa attggcatct cccagcggcc tgatggccgg caattgctca atgtgctgac
      901 acagcgcctg cacgtcctga aagcctaaat tcactccttg cccggccata ggatgcacct
      961 gatgtgccgc atcgcccagt aacagcacat gcggcaaggc catggtgtct gccgtatttt
     1021 gtaccagcgg gaaagccaga atatccccgc tgggcgtcaa ttggcccagg acatgcccac
     1081 aacgtgctcc aacttctgca gccaaggcct ctgctgccaa tgcctgcttt tgtgcggcgt
     1141 ccgcatgtgg tagcgcccaa accagcgata cctgctgttg cggcatgggc agtaaagcca
     1201 gtgtttcatg ggtgccaaac cactgcctgg caatgttgtc gtgtggccgc tcggcacggt
     1261 aattggtgac cagtgctgtc tgcgcaaagt cgacctgatg tacatgcacg ccacattgct
     1321 cacgcaccca tgatcgcccg ccatcagccc ctatcaacaa cccagcctta acctcatgtt
     1381 gcggcaagga taaccgggca acatgaccgg aatattgcaa ggcttgcgcc ggtgcgtcgg
     1441 tgatcacagt cacctctgca tgccttagga cttgccagca tgcattgatc aacgcctggc
     1501 tttcgacaat aaaccccatt tcgcttaaat gggcttcgtc cgcatccagg cgaagattag
     1561 gcgttgcatg actgtgtgtg ggtgaccaca aatgcatggc ggcaatcgga ttgatccggc
     1621 tggcaggcat gtgcgaccaa atgccaaggc tacgcaacca gtccaccgtt tgattagtca
     1681 gtgcataaat cctgctatcc cagtgattca catcgtcagc ccagttctcg gccaaagatt
     1741 gcgcaggttt ggcatccgtc aacacgattt ttttgccaag tttcgacagt gcaattgctg
     1801 ccgtcaagcc caccaggcct gcacccacaa tgaccacatc agttttaacc aggggaggat
     1861 taactgccat aactcatctt gttcacaagg aaacggcgtg ccatcggcaa catgctgagc
     1921 gctcccagac ccgcaccgcg taacaaaccg acaccagcca aatcattgga aaacacattc
     1981 accagaaaat ccgtgaactg caggccgcga ccggtatcgc gctgacgtga ctggtgatat
     2041 tcagtcaacc gatgtacagc accccattca ccggcctggg tagatgcgac atttttcgct
     2101 aatgccacgg cgtcacggat ccccacatta aatccctgtc ccgccaccgg gtgcatggtt
     2161 tgtgccgcat tgcctatgac cacacaatgc gccgcaaacg tttggcccag tttgctcagc
     2221 ttaagcggaa acttgaagcg tttgccaata tgcaagaatt tgcccacgcg atcgccaaac
     2281 gctgcgtgca aagcctctag aaaagcggca tcctctaatg ccgcaatgtc atcgatatac
     2341 gtttgctcac ctgtccaaac cagcgagaat tgtttcccgt tcggcaataa tgccatcgga
     2401 ccatgcggtg taaaccgctc ataagcaata ttgtgatgtg gttgctcggt ctgcaccagg
     2461 ccaaccagtg catcgtgccc gtaatgcttg gtttcccgca cgaggcctgg aatcgcttcc
     2521 aggcttcggc caccatcggc aatgaccgca atgggcgtcg ataattgttg cactgaacca
     2581 tccgcaagcg agtattgaat atcagctgca cctgcatgta catccaagcc tgttactggt
     2641 gcctgatact gaatattgat acctgcagcc gattgctcga tacgcgcatc cagcgcctgc
     2701 attaacgcgc cgtaagagac gacatagccc aaagcgggca acgcgtgctc gttcgcttta
     2761 aggacagtgc gcccaaagcc tttctgttgc gaaacatgaa tcgtttcgat ggctgtaact
     2821 gccgtttcca gcaatgccca gacacctagc tgctccagca cctgacgcgt accataagac
     2881 agtgccagcg ccctgccatc tccatacatg gctcctgcag actgtgcttc gagcacggtc
     2941 acgggcacac tggattgttg cagcgacaac gccagcacca gccccaccgg gccgccgcct
     3001 atcactacag gatgactgag ggttttcatg gcgtttgttg ccttttaatc cgtctataca
     3061 caatcaactt ttatgactgc cgcatcaatg cttcaatctc tgccaccgtt ttgatggcgg
     3121 cagacgtgat cacttcatgg ccagtgctgg tcaccagcac atcatcttca atgcggatcc
     3181 cgatattcca gaaatgctca ggcacattct ctgctgggcg cacatagcaa ccaggctcta
     3241 ccgtcagcac attgccaggc tgcagcatga cccaatcacc cgctggcgtt ttatattcgc
     3301 cagcatcatg cacatccagc cccagccaat gcccggtacg atgcatgtaa aactggcgat
     3361 aactgcctgt ttccagcacg gcttcccggc tgccctggca taaaccataa tggataaacc
     3421 catcaaccag aacctccaac gccgcctcat gtggtgcatt ccaatgatgc tgtggactga
     3481 cactcgcaat cgcagccgct tgggcggcga gcaccagctc atataaatcc ttttgcggac
     3541 cactgaaacg tccactcacc gggaaagtac gtgtaatgtc agaagcatag ccatccagct
     3601 cacaaccggc atctatcagt aacaggtcgc cagcttttaa ctcagcatta ttcgcgttgt
     3661 aatgcaatgt acaggcattg gcaccgcctg ccacgatgct ggtgtaagcc ggcgcctgtg
     3721 caccctggcg gtaaaattca tgcaaaaact ccgcttcaat ttcgtactcc cacttgccag
     3781 gaatcaccgc ttgcatggca cggttatgcg ctgcggcggc aatatgagcc gaacgacgca
     3841 tgagatcaat ttcatcaggg gatttaagca gacgcatggc gtccagcgct tgacgcacat
     3901 caaccagctt gcctggcgcc tgcacacctg tgcgggcttg cgccttgact gcattcaacc
     3961 agccgcttat tttagtatcc cagccagcgt ccgcacccag gctaaaaaac agttccggct
     4021 gatttgccat cagcttaggt aactgttcgt ccagttgcga gatggaatac gcttcatcaa
     4081 aaccgaattc gctttttgcc gcctccgggc cataacggaa tccgtcccaa atttcccgct
     4141 caatgtcttt atcgcggcaa aacaggatac ttctaggctg cgcacctgca atcaatacca
     4201 aaacggcttc gggctcttta aacccggtca ggtagtaaaa gtagctgtca aaacgataag
     4261 gatagtggct atcacgattg cgcgtcactt cgggcgcagt aggaatcacg gcgattccgg
     4321 cctgcatgct ggtcatcagt tgcaggcgtc gacgtgaaaa ttcttgcatg gaggtactca
     4381 aagcaaatcc ttggcaatca atctggtaaa gcggttgggc attgtagcgt atttggtatg
     4441 caattgctgg aatggttccg ccaggcaatg ctcgtcagaa ccccatcatt tcatataatt
     4501 ctgactgtct gacatttctt tcagcatcct ggatcgtgcc atcgctttgc cattgcttca
     4561 gtagtttatc gaggactggc cgcacctgct gagcacgcat gcccggcttg accgctgggg
     4621 cgtagagcat tttcagtagc aacgcatcta gcccagtcag taatgactcg ggcgttttat
     4681 cattaaatat agagggatag accttctcgg aatcattcgg caagcccatg gcctgtgtgg
     4741 tttcttcaac gatgcaggac aatagctggc gatgttcctg ggcaaaatcc accggaatca
     4801 ccacccaggc ttttttaatc gcctttttat catcaagatc cacaccgaac atacaagcgg
     4861 cgtcagcagg tggcttgatt gataaatttc cgctcaccag tgcgatgtca ttacgccaaa
     4921 gctgcaaatg tgagaacacc acaataaagt tagcctcctg gcgagacttg gcaaaaccaa
     4981 ttggatggcc ggtaatttta gccaaatgct gcatgtgata ttttgccaac agggcgtgtt
     5041 gttcagcctg gtctgtgtga tggtccaacc agacccggat cggttgtagc cacttgcgta
     5101 caggctgctc tccgacagcg tactcattgt gcaaagctac ttcaaaaaat gcctgctgaa
     5161 gatattcgga agactgccag gcttcttctg ccatcgcagc atgtatggtc agcaaaccaa
     5221 cgcaaaacca caagcagcga tcaatcattc agcttacccg cttcagcacg caatggcgtt
     5281 aatagtgagg acaaaccatt gtggtcaatc tcacgcatca aatgtaacag ccgccccaac
     5341 tcaccaggcg ggaatccctc acgtgcaaac cagtgcagat aatgtcctgg caagtctgca
     5401 atcagcctgc cttggtattt cccataaggc attttcagcg tcaccagc
//
LOCUS       DATMSK010000225         7206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_464115,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000225 DATMSK010000000
VERSION     DATMSK010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464115"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..2590
                     /gene="rnr"
                     /locus_tag="VNZ84_07525"
     CDS             116..2590
                     /gene="rnr"
                     /locus_tag="VNZ84_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HWU66845.1"
                     /translation="MSTRKTKKSLRANDPQAKREAELYQTPLPSRELVLQLLSDQGVP
                     LSADQIYLLLDISHDERDMFNRRLGAMERDGQLMRNRKGAFCLPDKIHLIAGTVFGHT
                     DGYGFLVPDDKTKNPEDIFLGPKEMTLVMHGDRAMVRLAGVDRKGRPEGKLVEVLERA
                     NKTLVGRVIRAQGVTIVAAEDKRISQDVLIPYHLDMDAQVGQVVTVELTEYPSSHSNP
                     MGKIVEILGNYADSGMEIEIALRKHHLPHQFNPAAIKQAEDFPRTVQAKDYKGRKDLR
                     DLPLITIDGETARDFDDAVFAEPQGKGWRLVVAIADVSFYVQPGDALDKEAYDRGNSV
                     YFPRRVIPMLPEALSNGLCSLNPDVERLCMVCDMQIDGQGVVKQYQFYPSVMLSKARM
                     TYTKVAALLQGEDAALSEEYAWLLPHLQNLNSVFRLMLTQREKRGAVEFESTETMMMF
                     NDNGKIDKIVPVVRNDAHKLIEECMLAANVCAADFLLKNEQTCLFRIHEGPTPEKLEA
                     LRLFMGEFGFGVGGGENPSAKDYAKLMQRIKARPDAQLLQTVMLRSMQQAVYSPDNVG
                     HFGLAYEAYTHFTSPIRRYPDLLVHRAIKAVVEGKRYTAKDWHALGTHCSMTERRADD
                     ATRDVTNWLKCFYMQDKVGEVFSGTVAGVTSFGLFVALDEVYVEGLLHVTDLGNDYFI
                     HDKARHEMVGERTGVRYRLGDRLTVKVVRVDLETSRIDFTLVGADDSQADAESGVTDE
                     GLAKPARKQARVAKSTVASTEKSASAVWGKSSAAATAKPASKSPRRAADKPVAGKPAA
                     GKSRSAKQKGRHSTQKNKGKPKAKVR"
     gene            2728..3489
                     /gene="rlmB"
                     /locus_tag="VNZ84_07530"
     CDS             2728..3489
                     /gene="rlmB"
                     /locus_tag="VNZ84_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWU66846.1"
                     /translation="MSETRILFGFHAVLSRMRQHGNSIQEIIIDQERVDARMRDLLEM
                     AKAQNIRVIQAERQRLDGFIGAHGRHQGVIARVVETPMPYKDIHDILESDLDEPPFFL
                     ILDGVQDPHNLGACLRVADAMGVHAIIAPKDRSVGLNGTVRKVASGAAETVPFIAVTN
                     LARTMRELKEAGVHIVGTTMDAPGTLHNSKLEGPIALVMGAEGDGMRRLTQETCDSLI
                     TIPMFGSVQSLNVSVASGIALYEIRRQRVLAGGKA"
     gene            3499..5274
                     /locus_tag="VNZ84_07535"
     CDS             3499..5274
                     /locus_tag="VNZ84_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU66847.1"
                     /translation="MLTLRDLWQQLRQQSSRLWLGQWLAFTGAVVAVPIPLLMPLLVD
                     EVLLHQPGKLTQFMRWLLPQEWLGPISIIIAVTLLTISLRLSSVLLGVATTRIFVGLS
                     KQVTLGVRQTLLDKLSRIRMQVYESLGAGQVTARLLTDIETLDKFLGETIAKVLVAVL
                     SVVGVAVVLLWMHWQLALIILLLNPFVIALTMMMGKKVKAWKQRENTAFEMLSQSVTE
                     TLEVMQQLRASNSDKRFLDKAKQAAEAVRVAATASGWKSDALSRLSFGVFLFGFEIFR
                     AVAMLMVVFSNLSVGQMIAVFGYLWFMMGPVQELLSVQVSYYAAKAALGRINQVLAAE
                     EEPHYPATVHAFSGQAPAAISLQQVTFAYGEGDAILADVSLNIAPGEQVALVGVSGAG
                     KSTLVQLLLGLYAPNQGQVLFNGQAIEQLGYAQVREHIGVVLQHPMLFNDSVRQNLSL
                     DLPYADADLWQVLEVAQLAQFVRDLPQGLDTSLGRSGVRLSGGQRQRLAVARMMLKQP
                     QVVILDEATSMLDTHTEALLHQAMRKFLRGRTTIIIAHRLSAVKEADRVLVFDGGHII
                     EEGQHETLVKRTGSVYQCLYGQQIA"
     gene            complement(5307..7073)
                     /locus_tag="VNZ84_07540"
     CDS             complement(5307..7073)
                     /locus_tag="VNZ84_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl cyclase"
                     /protein_id="HWU66848.1"
                     /translation="MRSFLPDYSTFKSPIFKTLSTSFAALLIVGCQETEAHKTQPAIP
                     DFGQNVIIIDPGMPSADIQHRLDALFKQQEANQFGPERYAVLFKPGVYHNKIKLGFYT
                     QLLGLGQFPDDVMLQGGIQVEAQWHEEDATQNFWRSVENLSVTPDNGTMQWAVSQAAP
                     LRRIHVRGNMLLDDNGGWSSGGFIADSLIDQQINSGTQQQWLTRNSTLGSWTNANWNM
                     VFVGVKNAPDAAHWPNPPYTVVDQTPVIREKPFLTIDQTGAYQVFVPALHTNTQGVSW
                     QSAHPEGENLPIDQFYIAKAANDTSDSLNSALKRGKHLLLTPGIYKLDKALQIDRPNT
                     IVLGLGIATLQPVCGNVAMTIADVDGIKLGGILFDASEKNSETLLQVGERKSTASHAN
                     NPTTLHDVFFRVGGAAVGNATHSVIVNSNHVIGDNLWVWRGDHGNGIGWASNKTKHGI
                     IVNGDDVTMYGLFVEHFHDYQTVWNGENGAVYFYQSEAPYDVPNQQSWMNGGVNGYAS
                     YKVGDHVTRHKATGMGVYCFFNENPSVKLNSAIEAPSGPDIHFSHVTSVSLGGTGEIT
                     HVLNDQGDKSYDKNYVSRIVGQ"
BASE COUNT         1687 a         1691 c         1997 g         1831 t
ORIGIN      
        1 cccagaagag gactcgaacc tccacgtcgt taaacactag tacctgaaac tagcgcgtct
       61 accaatttcg ccatctgggc taaagaagct gtgcatttta aaggaagaat aaatattgtc
      121 aaccagaaaa acgaaaaaat ccctgcgtgc aaatgacccg caggccaagc gagaagctga
      181 gttataccaa accccgttac cgagccgtga gctggtattg cagttgctct ctgaccaggg
      241 agttcccctt agcgctgacc agatttacct gttactggac atcagtcatg atgagcgcga
      301 catgttcaat cgcaggctgg gtgccatgga gcgagatggc cagttaatgc gcaaccgcaa
      361 aggtgccttt tgcctgccgg ataagataca tctgatcgct ggtaccgtgt ttggccatac
      421 agatggttat ggctttttgg tccctgatga taaaaccaaa aatcctgaag atatcttttt
      481 aggccctaag gaaatgaccc tggtcatgca tggcgaccgt gccatggttc ggttggctgg
      541 tgtagatcgc aaaggtcgtc cagaaggtaa gctggttgag gtactggagc gcgccaataa
      601 aaccttggta ggccgtgtga ttcgcgcaca aggggtcacc atcgtcgcgg cagaagataa
      661 gcgcatcagc caggatgtcc tgattcctta ccacctggat atggatgcgc aagtggggca
      721 ggtggtgacg gttgagttga cagaatatcc ctcatcacac tctaacccta tgggcaaaat
      781 tgtcgagatt ttaggcaatt atgcggatag cggcatggag atcgagattg ccttgcgcaa
      841 gcatcatttg ccgcatcagt ttaatcctgc ggcgatcaag caggcagaag actttcctag
      901 aacggtacaa gccaaagatt acaagggccg caaagatttg cgcgatttgc cattgattac
      961 cattgatggt gaaaccgcac gtgactttga tgatgccgta tttgccgagc cgcaaggcaa
     1021 aggctggcgc ttggtggtgg caattgccga cgtgagtttt tatgtacaac cgggagatgc
     1081 gctggataaa gaggcctatg accgcggtaa ttccgtctat ttcccccgcc gtgtcattcc
     1141 gatgttgcca gaggctttgt ctaatggctt atgttcactg aatcctgatg tcgagcgctt
     1201 gtgcatggtg tgcgatatgc agatcgacgg tcagggcgtg gtcaaacaat accagtttta
     1261 cccatcagtt atgctttcaa aagcgcgtat gacgtatacc aaagtggccg ccttgttaca
     1321 gggtgaagat gctgcgttaa gtgaggaata cgcctggttg ctgccgcatt tgcaaaatct
     1381 caacagcgta ttccgcctca tgctcaccca gcgtgaaaag cgtggcgcgg tagagtttga
     1441 gtctactgaa accatgatga tgtttaacga taacggcaag atcgacaaga tcgtgcctgt
     1501 ggtacgtaat gatgcgcaca agttaattga agagtgtatg ttggcagcca atgtctgcgc
     1561 agctgacttt ttgctcaaga atgagcaaac gtgcttgttc cgtattcatg agggtccaac
     1621 accagaaaaa ctggaagcgt tgcgcttgtt tatgggcgaa ttcggttttg gcgtcggcgg
     1681 tggtgaaaat ccgagtgcca aagactatgc gaaactgatg cagcgtatca aggcgcgccc
     1741 ggatgcacaa ctgttacaga cggtcatgtt gcgctccatg caacaggcag tctacagccc
     1801 ggataatgtc ggccactttg gcctggctta tgaggcgtat acccacttta cgtcaccgat
     1861 tcgccgttat cctgacttgc tggtacaccg tgccattaaa gccgtagtag aaggtaaacg
     1921 ctacactgcc aaggattggc acgcactcgg gacgcattgc tctatgactg agcgtcgtgc
     1981 cgatgatgcc acccgtgacg tcactaactg gctgaaatgc ttttacatgc aggacaaggt
     2041 cggtgaagta tttagtggta cggtagcagg tgtcactagc tttggcttat ttgtcgcgct
     2101 ggatgaggtg tatgtcgaag gcttgttgca tgtgactgac ttgggcaatg attatttcat
     2161 tcacgataag gcgcgtcatg agatggtcgg tgaacgcaca ggtgtacgat atcgactggg
     2221 tgaccggttg accgttaaag tggtgcgcgt ggatctggag acttcacgca tagactttac
     2281 gctggttggt gcggacgata gccaggccga tgcagaaagt ggcgtgaccg atgaagggtt
     2341 agcaaagccc gccagaaaac aggcacgggt cgctaaatcg acggtggctt caacagagaa
     2401 gtctgcatca gcggtctggg gtaagtcgtc ggcagcggct actgccaaac cagcgtccaa
     2461 atctccgcgc cgagcggcag ataaacccgt tgctggcaag cctgcagcag gtaaatcacg
     2521 ctcagcaaaa cagaagggcc gccattcgac gcaaaaaaat aaaggcaaac ccaaggcaaa
     2581 agtccgataa tagtcactgg agtcaccatt caggtgatga agtaatcaat gcatgcagca
     2641 atcagaatca ttctgcttgc tgcatgattc gttttaaact tttgcatttg cttagcaaga
     2701 gtcatcaaga gaaatttagg agtcactatg tcagaaaccc gcattctgtt tggttttcat
     2761 gcggtgttga gtcgcatgcg tcagcatggc aacagcattc aggaaattat tattgatcag
     2821 gaacgtgtcg atgcgcgtat gcgtgacctt cttgaaatgg ccaaggcgca gaatattcgc
     2881 gtgatccagg ccgaacgcca gcgcctggat gggttcatcg gtgcgcatgg ccgccatcag
     2941 ggcgtgattg cccgcgtggt tgaaacgccc atgccttaca aggatattca cgatatcctg
     3001 gagtctgatc tcgatgagcc accatttttc ctgatcctag acggtgtgca ggatccgcat
     3061 aacctgggcg cttgcttgcg tgtggcggat gccatgggtg tgcatgccat cattgcacct
     3121 aaggaccgtt ctgtgggttt gaatggcacg gtgcgcaagg tggccagtgg tgcggcagag
     3181 acggtgcctt ttattgctgt cactaacctg gcgcgcacga tgcgtgagtt gaaagaggcg
     3241 ggcgtgcata ttgtcggcac caccatggat gcaccgggga ctcttcacaa tagcaaactg
     3301 gagggaccaa ttgcgcttgt catgggcgcg gaaggcgatg gtatgcgccg tttgacgcaa
     3361 gaaacctgtg attcgctgat taccattccc atgtttggat cggtacagag tttgaatgtg
     3421 agtgtggcga gcggcattgc cttgtatgag atccgccgcc agcgcgtact ggcaggtgga
     3481 aaagcttagt tttaccctat gttgaccttg cgtgacttgt ggcagcagtt gcgccagcaa
     3541 tctagccgtt tatggctggg ccagtggctg gcatttaccg gggctgttgt cgctgtgcct
     3601 attcctttgc tgatgccttt gttggtggat gaggtgctac tgcatcaacc aggcaagctg
     3661 actcagttta tgcgttggtt gctgccgcag gagtggctgg gacccatcag catcattatc
     3721 gctgtgacac tcctgaccat tagcctgaga ttgagcagcg tgctgctggg ggtggccacc
     3781 acacgtattt ttgtaggctt atccaagcag gtgacgttgg gtgtgcgcca aacgctgctc
     3841 gacaaactct cgcgcatccg tatgcaagtc tatgaaagcc tgggtgcagg gcaggtgacg
     3901 gcaaggttgc tcacggatat tgaaaccctg gacaagtttt taggcgaaac cattgccaag
     3961 gtattggtag cggtacttag cgtggttggt gtggcggttg tattgttatg gatgcactgg
     4021 caactggcgc tgattatcct gctactgaat ccgtttgtga ttgcgttgac catgatgatg
     4081 ggtaaaaagg tcaaggcctg gaaacagcgt gaaaacactg cgtttgaaat gctgtcgcag
     4141 tcggtgactg aaaccctcga agtcatgcag caattacgcg caagtaatag tgacaagcgt
     4201 tttctggata aagccaagca agcggcggaa gcagtcaggg tagcggcaac cgcttctggc
     4261 tggaagtcag atgctttgtc acgtttgtct tttggtgtat tcctgtttgg atttgaaatc
     4321 ttccgggcag tggccatgct gatggtcgtt ttttccaacc tgtcagtcgg gcagatgatc
     4381 gcggtgtttg gctacctgtg gtttatgatg gggccggtgc aggagctgct gtcggtacag
     4441 gtttcctatt acgcggctaa agccgctctc ggaagaatta accaggtgct cgccgcggaa
     4501 gaagagcctc attatcccgc tacggtacac gcgttttctg gtcaggcacc cgcagccatc
     4561 agtttgcaac aggtcacttt tgcttatggt gaaggtgacg ctatcctggc tgatgtctca
     4621 ttgaatatcg caccaggcga gcaggtggct ctggtgggag tctccggcgc aggtaaatct
     4681 acgctagtac agttgctgtt ggggttgtat gcgcccaatc aaggccaggt tttgtttaat
     4741 ggtcaggcga tagagcaatt gggctacgcg caggttcgcg aacacattgg ggtagtattg
     4801 cagcatccca tgttgttcaa cgatagtgtg cggcagaatc tgagtctgga cctgccgtat
     4861 gcagacgcag acttatggca ggtgctggaa gtcgcgcagc tcgcacagtt tgtaagggac
     4921 ttgccacaag gcctggacac atcgcttggc cgcagtggtg tacgcttgtc aggtgggcag
     4981 cgccagcggt tggcggttgc acgtatgatg cttaagcagc cacaggtcgt gattctggat
     5041 gaggcaacct ccatgctgga tacacacacg gaggctttgc tacatcaagc gatgcgcaag
     5101 tttttacgtg gacggacgac gattattatc gcgcaccgtt tgtctgctgt gaaagaagcg
     5161 gatcgcgtgc tggtgttcga tggcggccac attattgagg aaggccagca cgaaacactg
     5221 gtgaagcgta ctggcagtgt ttaccaatgt ttgtacgggc agcaaatagc ctaaacagcc
     5281 ataaacaacg cattcagacc gacgatttat tgccccacga ttcttgacac ataattcttg
     5341 tcataggatt tgtcgccttg gtcattgagt acatgtgtga tttcacctgt tcctcccagt
     5401 gaaacactgg tgacgtggct aaagtggata tcagggcccg agggcgcttc aatcgcgctg
     5461 ttaagtttga cactggggtt ttcattgaaa aagcaataaa cccccatgcc ggtggctttg
     5521 tggcgggtca catggtcgcc cactttgtaa gaagcatagc cattgacgcc tccgttcatc
     5581 cagctttgct ggttaggcac atcataaggt gcttcgctct ggtaaaaata gaccgcgccg
     5641 ttttcaccgt tccagaccgt ttggtaatca tgaaaatgct cgacaaataa accatacatg
     5701 gtcacgtcat cgccattgac gataatccca tgcttggttt tgttgctggc ccagccgatg
     5761 ccattgccat gatcgccgcg ccacacccac aggttgtcgc caatcacatg gttgctgttc
     5821 acgatcacgc tgtgtgttgc attccctacg gctgcgccgc ccaccctgaa aaatacatca
     5881 tgtagtgtgg tggggttgtt ggcatgactt gctgtgctct tacgttcacc cacttgcagc
     5941 aatgtctctg aattcttctc actggcatca aataatatgc ccccgagttt gattccgtcg
     6001 acatctgcaa tggtcatggc aacattgccg caaaccggtt gcaaggtagc gatgcccagg
     6061 ccaagcacaa tagtatttgg acgatctatt tgtagtgcct tgtccagttt gtaaatgcct
     6121 ggggttaaca gtaggtgctt gccccgcttt aaagccgaat taaggctgtc agaggtgtcg
     6181 ttggctgctt tggcaatgta gaactggtca atgggcaagt tctcaccttc tggatgagca
     6241 ctctgccagg aaacgccttg agtattggta tgcaaggcag gcacaaatac ctggtaagca
     6301 cccgtttgat ctatcgtgag gaaaggtttt tcacggatca ccggtgtctg gtcaacgacg
     6361 gtataaggtg gattaggcca atgtgctgca tccggcgcat ttttaacgcc gacaaatacc
     6421 atgttccaat tggcattggt ccaactgccc agtgttgagt tcctggtcag ccattgttgc
     6481 tgcgtgccgg agttgatttg ctggtcaatc aggctgtcag caataaaacc gccactggac
     6541 cagccaccgt tgtcatccag caacatattg cctcgcacat gaatccggcg cagtggagcc
     6601 gcttgtgaaa cggcccattg catggtgcca ttgtctgggg tcacagacag gttttcaacg
     6661 ctacgccaga aattttgcgt agcatcttct tcatgccatt gggcttctac ctgtatgcca
     6721 ccttgtaaca tcacatcatc aggaaattgc cccaggccca atagttgcgt gtaaaaacct
     6781 agtttgattt tattgtggta cacgcctggc ttgaacaaaa cggcatagcg ctcagggcca
     6841 aattggttgg cttcttgctg tttgaagagt gcatccaagc gatgttgaat atcggccgat
     6901 ggcatgcctg gatcaataat gatcacgttt tggccaaaat ctggaatggc aggctgcgtt
     6961 ttgtgtgctt cggtttcctg gcaacccacg atcagcaggg cagcgaaact ggtgctcagt
     7021 gttttgaata ttgggctttt gaaagtggag taatcaggca aaaacgaacg catagacagg
     7081 tggtttccga gagaaatcat ttatacatga ccgcgatagg caagatgaaa ttcaattttt
     7141 tttcatgttt caccccgttt tctttcaata atactgtttg cattgagtgc ccccaaggcc
     7201 acaacg
//
LOCUS       DATMSK010000226         6213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_369927,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000226 DATMSK010000000
VERSION     DATMSK010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369927"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1045)
                     /gene="tsaD"
                     /locus_tag="VNZ84_07545"
     CDS             complement(23..1045)
                     /gene="tsaD"
                     /locus_tag="VNZ84_07545"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWU66849.1"
                     /translation="MLVLGIESSCDETGVALYDTRRGLLAHALHSQIDMHAAYGGVVP
                     ELASRDHIRRALPLTQQVLAHADKTLQDIEGIAYTQGPGLAGALLVGTSIAQSLAMAL
                     NVPSIGVHHLEGHLLAPMLEENPPAFPFVALLVSGGHSQLMRVDGIGQYELLGDTLDD
                     AAGEAFDKTAKLLCLGYPGGPAVSKLADQGKPHFQLPRPMLNSKDLNFSFSGLKTAVL
                     TLVNNQPELDDDTVKDIAWEFQESVTEILVHKCLKALKQTKLKRLVVSGGVGANKRLR
                     TRLNEAASKRGISVSYPRLEFCTDNGAMIAFAGAMRLHAMQPGKQDYSFSIRPRWDLA
                     ELKAPA"
     gene            1166..1378
                     /gene="rpsU"
                     /locus_tag="VNZ84_07550"
     CDS             1166..1378
                     /gene="rpsU"
                     /locus_tag="VNZ84_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWU66850.1"
                     /translation="MTAIRVKENEPFDVAMRRFKRLIEKTGLLNDLRAREFYEKPTWE
                     RKRKAAAAVKRQYRRLRNQQLPTKLY"
     gene            1496..1939
                     /locus_tag="VNZ84_07555"
     CDS             1496..1939
                     /locus_tag="VNZ84_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWU66851.1"
                     /translation="MSLKAQISEDMKTAMRAKDMQKLGVIRLLQAAIKQREVDERIEL
                     DDDAVIAAIEKMLKQRRDSIAAFEGANRMDLADQEKFEVTVLQAYLPAQLSEEEINAI
                     IAKVVADTGAAGAKDMGKVVGAVKPLVAGKADMAKVSGLIKAALG"
     gene            2017..3756
                     /gene="dnaG"
                     /locus_tag="VNZ84_07560"
     CDS             2017..3756
                     /gene="dnaG"
                     /locus_tag="VNZ84_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWU66852.1"
                     /translation="MIPESFIQELLNRIDIVEVIDKAVPLKKAGANYSACCPFHNEKS
                     PSFTVSPTKQFYHCFGCGAHGSALSFLMEYNGLSFVEAIHDLARQVGMIVPQEQRDPN
                     QPAPASKAVLLSLQETLQQAANYYKAELKQSQRAIDYLKARGLSGQVAAKFQIGYAPA
                     GWQNLQTVFPQYDAEPLETAGLVVQNEQGRRYDRFRDRIMFPIHNQKGEVIGFGGRVI
                     NPEDSPKYYNSPETPVFQKGHELYGLFMARRAIRDAGRVLVVEGYMDVVALAQYGIEY
                     AVAALGTATTPFHIAKLMRQTDEIVFSFDGDKAGRTAAWRAVMNALPAIKDGVKLRFL
                     FLPAEHDPDSFVREFGKEAFEAEMDKAMPLSQYIIQHLSEHNPLASQEDKVQFLNDAE
                     PLLKQIQAPRYAMLLRKKVAEMTGLAGGEMQRMLKLPNPQKVQEKAVRQRARTPLSLH
                     VRLALMLLMRPQWAKTELADNVAGESDEEQMLRVVIRAALAKPESRPVVLLQMISPYI
                     SESLRHQLQRELSLLDDSLDFALEFEGACTQLSEMAVIAREKSLLGRLTEKPFSALSD
                     AEREALRQLTSKR"
     gene            3906..5855
                     /gene="rpoD"
                     /locus_tag="VNZ84_07565"
     CDS             3906..5855
                     /gene="rpoD"
                     /locus_tag="VNZ84_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWU66853.1"
                     /translation="MARPKADKSLDKELGINEVETPKDPATQALDAEARRTRLKTLIV
                     LGKERGYLTYAEINDHLPDDVQDSEQIESIIGMINDMGIQVYEEAPDAEVLLMSDAPP
                     AVTDDDAAEEAEQALATVDSEFGRTTDPVRMYMREMGTVDLLTREGEIEIAKRIEDGL
                     KHMVQAIAACPTTIAQLLEMVDKVEKDEMSVDELVDGLIDSDLGLDDALAVADAEEEE
                     AADEEDEDEDEDGDAKASALSAEALARLKDEVLSRFAVIRAANVKMNTIREDKGTSDN
                     EYKTLHQQVLDELTAFRFSAKQVEALCDQVRSMVEEVRTHERKIMEFCVDKANMPRQQ
                     FIKSFVGNEINLNWLDDELAGKPAYAERLERFRHSILDQQERLQNLQGRVGLPIKELR
                     EINKQMTTGEARARRAKREMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVD
                     KFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQETG
                     LEPDPATLAEKMDMPEEKIRKILKISKEPISMETPIGDDEDSHLGDFIEDNTTLAPMD
                     AAVYASLRDATSEVLESLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIE
                     AKALRKLRHPTRSERLRSFLENGQD"
     gene            5876..5954
                     /locus_tag="VNZ84_07570"
     tRNA            5876..5954
                     /locus_tag="VNZ84_07570"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5912..5914,aa:Ile,seq:cat)
BASE COUNT         1593 a         1438 c         1704 g         1478 t
ORIGIN      
        1 caagaaataa tccggctaaa atttaagctg gggctttgag ttcagccaaa tcccagcgcg
       61 gccggatgct aaagctgtag tcttgcttgc ctggctgcat ggcgtgtaat cgcatggcgc
      121 cggcaaaggc aatcatggcg ccgttatcgg tgcaaaactc caggcgtggg taactgacgc
      181 tgatgccgcg tttagatgcc gcttcattta atctggtgcg caggcgttta ttggcaccta
      241 cgccgccaga gactactaag cgtttaagtt ttgtctgttt gagagccttc aaacatttat
      301 gcactaatat ctcggtcact gattcctgga attcccaggc aatgtcttta accgtgtcat
      361 catccagttc aggttggttg ttcaccaatg tcagtacagc ggttttgagg ccgctgaaac
      421 tgaagttcaa atctttgctg ttaagcatgg ggcgtggcaa ttgaaagtgt ggtttcccct
      481 ggtcggccag tttagatacg gcgggtccac cggggtaacc caggcacaat agtttggcag
      541 ttttatcaaa agcttcgcct gctgcatcat ccagtgtatc gcctaataat tcatattggc
      601 cgataccatc cacgcgcatg agttggctat ggccgccaga gactagtagc gcaacaaatg
      661 gaaaggcagg cgggttttct tccagcatcg gggccagtaa atgtccttct aggtggtgca
      721 cgcctatgga cggtacgttc agtgccatgg ctaacgattg tgcaatactg gtgcccacca
      781 gcaaggcgcc agccaggccg ggaccttgtg tataagcgat accttcgatg tcttgcaagg
      841 ttttgtcagc gtgtgccagc acttgctgcg tgagtgggag cgcgcggcgg atatggtcgc
      901 gtgaggccag ctctggcacc acgccgccat aagcggcatg catgtcgatt tgcgagtgaa
      961 gggcgtgcgc cagtaatccg cggcgtgtgt catacaatgc caccccggtt tcgtcacaag
     1021 aagattctat acctagcact aacatacgat tttttgcaga aaagattgat aaatccaaat
     1081 aagcgcgata gaatacgcgg ttttcagtgc tgcatcaatc cgcaagcacc attgtctaac
     1141 caataattga aggataggga tttacatgac cgctattcgt gtgaaagaga acgaaccgtt
     1201 tgacgttgca atgcgccgtt tcaagcgttt gattgagaaa actggtttgt tgaacgactt
     1261 gcgtgctcgc gagttctacg agaagccaac ttgggagcgt aagcgtaaag cggctgctgc
     1321 ggttaagcgt caataccgcc gtctgcgcaa ccaacagttg ccaacaaaac tctactaatt
     1381 tttagtggat gtgaacggtt agccgtcttc aggttaaccg atgatgcgaa ggttgtgggt
     1441 ttccgcaacc tttagctatt tatacagccc atatttatct ggaccaagac acgctatgag
     1501 cctgaaagca caaatctccg aagacatgaa aactgccatg cgcgcaaagg acatgcaaaa
     1561 actgggcgtg atccgtttgc tgcaagcggc gatcaagcag cgcgaggtcg atgagcgtat
     1621 tgagctggat gatgacgccg tcattgctgc gatcgagaaa atgcttaaac agcgtcgcga
     1681 ttccattgct gcgtttgaag gcgcaaatcg catggatttg gccgaccagg aaaaattcga
     1741 agtgacggtc ttacaagctt atctgccagc tcagttaagc gaagaagaga ttaatgccat
     1801 tatcgccaag gtagtggctg acacgggtgc tgctggtgcc aaggatatgg gtaaagtggt
     1861 aggtgctgtg aagccattgg tggccggtaa ggcagatatg gcaaaagtgt ctggcctgat
     1921 caaggcagca ttgggatagt ctgcggttgc agattgaagc atgagggcgc attggcgccc
     1981 tttgtttttt gttttgcatc agccatactt gcacctatga tcccggaaag ttttattcaa
     2041 gaactgctta atcgcataga tattgtcgaa gtcatcgaca aggcagtgcc actgaaaaaa
     2101 gccggggcca attactcggc ctgttgtcct ttccacaacg aaaaatctcc cagttttacc
     2161 gtcagcccga ccaagcagtt ttaccattgt tttggctgcg gcgcgcatgg ctctgcgctg
     2221 agttttctca tggagtataa cggcctgagt tttgttgagg cgattcatga cctggccagg
     2281 caggttggca tgattgtgcc gcaagagcag cgcgacccta accagcctgc gcctgcctct
     2341 aaagcagtgt tgctgagttt gcaggaaacc ttgcagcagg cggccaatta ttacaaagct
     2401 gaattaaagc aatcgcagcg ggcgattgat tacctgaaag cgcgtggttt aagtgggcag
     2461 gtcgcggcca agtttcagat cggttatgcg ccagctggtt ggcaaaacct gcaaaccgtg
     2521 tttccgcaat acgatgccga accgctggaa acggcgggtt tagtggtgca aaacgagcag
     2581 gggcgtcgct atgaccgctt ccgcgaccgt atcatgtttc caatccataa ccagaagggt
     2641 gaagtgattg gttttggcgg gcgcgtgatt aaccctgaag atagccccaa gtattacaac
     2701 tcgccagaaa caccggtgtt ccagaaaggc catgagttat acggcctgtt tatggcgcgg
     2761 cgcgccatcc gcgatgcagg ccgtgtgctg gtggttgagg gctatatgga tgtggtggcg
     2821 ctggcgcagt atggtattga atatgctgtc gccgcactgg gtacggccac cacgccattt
     2881 catatagcga aactcatgcg gcaaaccgat gaaattgtat tcagttttga tggcgataaa
     2941 gcggggcgta cggctgcctg gcgcgcggtg atgaatgcct tgccggccat taaagatggc
     3001 gtcaagttgc gctttttgtt tttgcctgca gagcatgatc cggatagctt tgtgcgcgag
     3061 tttggcaaag aagcattcga ggctgagatg gataaggcca tgccgctatc gcaatatatt
     3121 attcagcatt tgagtgagca taatcctctc gcgtcgcaag aggacaaagt gcagtttttg
     3181 aatgatgccg agcctcttct caagcagatc caagcgccac gctatgcaat gctgttgcgt
     3241 aaaaaagtgg ccgagatgac agggctggcc ggcggtgaaa tgcagcgcat gctcaaatta
     3301 cccaatccgc aaaaagttca ggaaaaagcc gtgcgtcagc gtgcgcgtac gccgttgtcg
     3361 ctgcatgtcc ggttggcgct gatgctgctg atgcgtccgc aatgggctaa gaccgagttg
     3421 gctgataatg ttgctggtga aagtgatgag gagcagatgc tgcgggtggt gatccgcgct
     3481 gcattggcca aacctgagtc acggccagtg gtgttgttac aaatgatctc cccttatatt
     3541 tcagagagtt tgcgacacca attgcagcgc gagttgtctt tgctggatga ctcgctggat
     3601 tttgcgcttg aatttgaagg ggcgtgtacc caattatcag agatggcagt gattgcgcgc
     3661 gagaagtccc tgttggggcg gctgactgaa aagccgtttt ctgcgttatc ggatgccgaa
     3721 cgtgaggcat tgcggcagtt gaccagcaag cgctagtcac cgctcccgaa aaaacccgga
     3781 ggatatgctt aaaaaacatg cacaaatctg gtaaaataaa gggtttcccg taatgttata
     3841 tcgagtggtg catcagaagt gttgatcagc attcgacaac aaccatttta gaggtggaat
     3901 cggccatggc cagaccaaaa gcagataaaa gtttagataa agaactcgga atcaacgaag
     3961 tcgaaacccc taaagatccc gctacccagg cactggatgc agaggcacgc cgcactcgcc
     4021 tgaaaaccct gattgtcctc ggtaaagagc gtggctacct gacctacgcc gaaatcaatg
     4081 accacctgcc ggatgatgta caggattccg agcagatcga gagcattatc ggcatgatca
     4141 acgacatggg tattcaggtg tatgaagaag cacctgatgc tgaagttttg ttgatgtctg
     4201 atgcgccgcc ggcggtgact gatgatgatg ctgccgaaga agccgagcag gcactggcaa
     4261 ccgtggactc tgagttcggc cgtacgactg accctgtgcg tatgtatatg cgtgaaatgg
     4321 ggaccgttga cctgctgacc cgtgaaggcg aaattgagat tgccaagcgt attgaagatg
     4381 gtctcaagca catggtgcaa gcgattgccg cctgtccaac cactatcgcc caattgctgg
     4441 agatggtaga taaagtcgaa aaagacgaaa tgagcgtcga cgagttggtc gatggtttga
     4501 ttgacagcga cttgggcctt gacgatgcgt tagccgtggc agatgccgaa gaagaggaag
     4561 ctgccgacga agaggatgaa gatgaagacg aagatggtga tgcgaaagct tctgccctgt
     4621 ctgctgaagc cttggcaaga ctcaaagatg aagtattgtc acgctttgca gtaatccgtg
     4681 cggcaaacgt caaaatgaat accatccgtg aggataaagg caccagtgat aacgagtaca
     4741 agacactgca tcagcaagtg ctcgacgagc taaccgcatt tcgtttttct gccaaacaag
     4801 tggaggcgtt gtgtgaccag gtgcgtagca tggtcgaaga ggttcgtacg catgaacgca
     4861 agatcatgga gttctgtgta gacaaagcca atatgccacg tcagcagttc atcaagagct
     4921 ttgttggcaa tgaaatcaac ttgaactggc tggatgatga gctggctggc aaaccagcct
     4981 atgcagaacg tttagagcgt ttcaggcact ctatcctgga ccagcaagag cgtttgcaaa
     5041 acttgcaggg ccgtgttggt ttaccgatca aagagttgcg cgaaatcaac aaacagatga
     5101 ctactggcga agctcgggct cgtcgcgcca agcgtgaaat gatcgaggct aacttgcgtc
     5161 tggtgatctc tattgcaaaa aaatacacca accgtggctt gcagttcctt gacctcattc
     5221 aggagggtaa catcggcttg atgaaagccg tggataagtt tgaataccgc cgtggttaca
     5281 aattctctac ctatgcgaca tggtggatcc gtcaggcgat tacacgttcg atcgctgacc
     5341 aggcgcggac tatccgtatc ccggtacaca tgatcgaaac catcaacaag atgaaccgta
     5401 tcagccgtca gatcctgcaa gagacaggtc tggaacctga tccggccacc ctggctgaaa
     5461 aaatggatat gccggaagaa aaaatccgca aaatcctcaa aatcagcaaa gagcccatct
     5521 cgatggagac accgattggt gatgatgaag atagccactt gggtgacttt attgaagata
     5581 acaccacact ggccccaatg gacgcagcgg tgtacgccag cctgcgtgat gccaccagtg
     5641 aagtgttgga atcactgaca ccacgtgagg ccaaagtatt gcgtatgcgc tttggtatcg
     5701 aaatgaacac cgaccacacc ctggaagaag tcggcaagca atttgatgtg acacgtgaac
     5761 gtatccgcca gattgaagct aaggccctgc gtaaattacg tcacccgaca cgctcggagc
     5821 gtctgcgcag cttcttagaa aacggtcagg attagtttat aatcccacgc tttccggccc
     5881 cttagctcat gcttggttag agcagcggac tcataatccg ttggtgctgt gttcgactca
     5941 cagaggggcc accaaaaatt gctgtaaatc aaagggttac gttaattcgt agccctttgt
     6001 tttttgtcat ctctgtaaaa agttcaaccg agaaataatt tcgctcatga attaagcata
     6061 aaagtctctt gtaagtgttc tgggaaaaga tgcatagaac gcattgtctc tgactaaatc
     6121 tgagatcaag gtggcgttta gggtgtactg ccggttctat tttctgtctt aaaaacgggc
     6181 agacactaaa aaaccgctaa cgcggaattt tag
//
LOCUS       DATMSK010000227         6140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_17183,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000227 DATMSK010000000
VERSION     DATMSK010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17183"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VNZ84_07575"
     CDS             <1..317
                     /locus_tag="VNZ84_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66854.1"
                     /translation="PVEKYVGTLGNGTRSEVWWIPALKLPYKVAYTYPHHTVSIQMQA
                     LQPAAKSEQPVEIAPGTSLTALTNYQHVDYADLGDMEHNPSEMQWLAKAHGAPGLQAH
                     HH"
     gene            476..1825
                     /gene="rmuC"
                     /locus_tag="VNZ84_07580"
     CDS             476..1825
                     /gene="rmuC"
                     /locus_tag="VNZ84_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWU66855.1"
                     /translation="MTLQIIQLLLLLLVIAMLVWQWRTQQHAAQQALAQLLSQQLEEK
                     HRLMMSEMHQSLNQLADRLQSLNADQQDRLRAQLSQELTATREAMQALQVAQRDHLSQ
                     SSEQLSLSLRNGLSEQGMLQQNLLKTALQTTTQSLTQSIEALTKVVDGRLEEMSGKVS
                     IRLEEGFKKTNETFVSVMERLATIDEAQKKIDGLSVNMVSLQELLGDKRSRGAYGEVQ
                     LEGLVRNVLPVNSFAMQHTLSNGARVDCALFLPEPTGTVAVDSKFPLENYHRMIDSSL
                     GELVQAQASKLFKADIKKHIDDISSKYIIANETSDGAVMFIPAEAVFAEIHAYHGDVV
                     DYAMQKRVWMVSPTTLMAVLNTARAVLKDVEMRKQVHIIKDELGKLSKDFDRFDIRMK
                     KLADNIRQAHENAQDVHISSQKISRRFAQIEKVELAQTAGLETLELLDELDEAGKSH"
     gene            1828..2085
                     /gene="moaD"
                     /locus_tag="VNZ84_07585"
     CDS             1828..2085
                     /gene="moaD"
                     /locus_tag="VNZ84_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HWU66856.1"
                     /translation="MKITILYFARIKEVVNYSSEDVVLPEGVETIVALKSWLSDRGDT
                     WQKLFNGSGVVRAAINHELVDDMATFDEGDEVAFFPPVTGG"
     gene            2048..2563
                     /locus_tag="VNZ84_07590"
     CDS             2048..2563
                     /locus_tag="VNZ84_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWU66857.1"
                     /translation="MKWHFFHPLPEAELSEAVYLNVSVQPEDFDTGHELQQLRAATPE
                     AGALVNFVGLVREISQRGQLSHMLIEHYPGMTEKALQQIAGQAAQRWPLLGVRMIHRV
                     GLLPAQGQIVLVAATSLHRQAAFEACAFMMDYLKTEAPFWKKEVGDFGEHWVEARASD
                     EQAKQQWKLPR"
     gene            2560..4728
                     /locus_tag="VNZ84_07595"
     CDS             2560..4728
                     /locus_tag="VNZ84_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU66858.1"
                     /translation="MIQVLSPNEMTVSYKLSPMTFESTEALLDAADLDLTDYGWKLQP
                     EAQKTLMMGLSLQQAGCNVLVLGTPGSGRAGLTLAAMKAAAVTGIDSSLTDLVALYDF
                     SQQSCAHYIKLPAGLGAQLRTVMESFIKALIAEMQQWLDASGQVNLPQAETWLEERVD
                     GLRSQLSSAKLSPFFNIIKPEVLAYLQAWQQVGNDGESSSDSIVNDSFLSRFRVNLLV
                     DQRANLTAGVKQPVIEEKDPGFAALFGMLETATGESAQWPEFLRLRAGSVHRADGGML
                     LLHLRDLMQDEGNGSALLEKLYRFMRNHEVQIEDWANHAQQGAGGNRHGTLPVHVKIV
                     MIASREDYYDCLEAQPDFFDFFPIKVEFEDRVSATLDNYAWIAGYIARKCQQQVLPHF
                     SGAAVNVMLQFMHRVEEDQGRISTRFAHLDQLMHESVAVATDARLVTDEHVKQALAAR
                     LLRQQFFETQIRDSIIDNELLIQVHGSVVGQVNGLTHIELGDASFGSPIRITARCYPG
                     RSGVINIDREVEMSGPTHDKGIYILQNWLSASFWALAPLSLNASLVFEQEYNGVEGDS
                     ASCAELFALLSALTALPIKQGMAVTGALNQFGEVMPIGGVNEKIEGYFRVCQALGLNG
                     DQGVIIPKRNQRHLILDESVVNAVNNGMFKIIAIDHVLEGIAHLTDCEAGVQEADGTY
                     QGDSLMARAQATLASYRRTLERNQARMADIQQQVESGKSL"
     gene            4735..5172
                     /locus_tag="VNZ84_07600"
     CDS             4735..5172
                     /locus_tag="VNZ84_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HWU66859.1"
                     /translation="MARSHNKRLDKKEQPDENQEDSCRLDKWLWAARFFKTRSLAAEA
                     VDGGKVRIDGDRAKNAREMKPGMRVSIKNKDMQIEVEVLALSNVRRGAPEAALLYQET
                     EESLRLRQQTKESGADQYAERDRGMGRPTKRQMREIQRFTGRG"
     gene            complement(5182..5961)
                     /gene="epsA"
                     /locus_tag="VNZ84_07605"
     CDS             complement(5182..5961)
                     /gene="epsA"
                     /locus_tag="VNZ84_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated transcriptional
                     regulator EpsA"
                     /protein_id="HWU66860.1"
                     /translation="MLAQKKHNYWEGILKAIQNSYAIKRHIEFFNWLKQDIHPLMRHD
                     VLLVAWGDFSNGDLHFDVSSSFPELTTQRLLEDSAIVNYLMCNLYKRWVENEDKWYIV
                     NRFDASGIHAQSPNAFTQQLNKMHSLLVYGVRDTRGKADCIYVFFDRTKEFHVPDQML
                     GMIMPHVDAAIRRVGHLTPVIKDEDLLDSMAQGGLSDREQEILHWVRSGKTNLEIGMI
                     LNISANTVKNHLKRIFQKLDVSCRAQAVAKTGIVAGTSRPS"
BASE COUNT         1527 a         1437 c         1709 g         1467 t
ORIGIN      
        1 acccggtaga gaaatatgtg ggtactttgg ggaatggcac ccgtagtgaa gtatggtgga
       61 ttcctgcgtt gaaattacct tataaagtcg cttataccta cccacaccat accgtgagta
      121 tccagatgca ggcactgcaa ccagcagcca agtctgagca gccggtagaa attgcgcctg
      181 gcacttcact gacggcactc accaattacc agcatgtgga ttatgcggat ctgggtgata
      241 tggagcacaa tccatcagaa atgcagtggt tagccaaagc gcatggtgcg ccaggcttgc
      301 aggcgcatca tcattaggcg cgccaggctt acagtcgcac actcattcag tccgcctgat
      361 ttgtgcaact cgtattaaac agattcattt actcgtttag tctttacccg catcctcgca
      421 gccgaatttg ctgcgagccg atgtggcgta ctcgcgcatc ggctataatg cggccatgac
      481 gcttcaaatt attcagttac ttctcttgct gctggtgatc gccatgctgg tctggcaatg
      541 gcgtacgcag caacacgcgg cacaacaggc gctggcacaa ttactttccc agcaactcga
      601 agaaaagcac cgcttgatga tgagcgagat gcaccagtca ctgaaccagc tggctgacag
      661 attacagagc ctgaatgccg accagcagga tcgtttgcga gcacagttat cgcaagagct
      721 gacggcgacg cgtgaagcca tgcaggcgct acaagttgca cagcgtgacc atctcagcca
      781 aagcagtgag caactcagct tgagtttgcg caacggtttg tccgaacagg gcatgttgca
      841 gcaaaacctg ctgaaaaccg ctttgcaaac taccacccag agcctgacac aaagcattga
      901 agcattgacc aaagtggtcg atggccgcct cgaagaaatg agtggcaagg tgtcgatccg
      961 tctggaagag ggattcaaga aaaccaatga aacctttgtc agcgtgatgg aacgtttggc
     1021 caccattgat gaggcgcaaa agaaaattga tggcctcagc gtcaatatgg tgagcctgca
     1081 ggagctgtta ggcgacaaac gtagccgtgg cgcctatggt gaggtacagc tggaaggcct
     1141 ggtacgcaat gtgttgccgg tcaacagttt tgccatgcag cacacgctca gcaatggcgc
     1201 gcgggtcgat tgtgccttgt tcttgcctga gccgacgggt acggtggccg tggatagtaa
     1261 atttccgctg gaaaattatc accgcatgat agacagtagt ctgggcgagc tggtacaggc
     1321 gcaggcgtcg aaattgttta aagccgatat caagaaacat atcgacgata tcagcagtaa
     1381 atatattatt gccaatgaaa cttcagatgg cgcagtgatg tttattccgg ccgaagccgt
     1441 gtttgccgag attcatgcct accatggtga tgtggtggat tacgccatgc aaaaacgtgt
     1501 gtggatggtt tccccgacga ccttgatggc tgtgctcaac acggcacgtg cggtcctcaa
     1561 agacgttgaa atgcgtaagc aagtgcatat catcaaagat gagctgggca agctgagtaa
     1621 agattttgac cgttttgata tccgcatgaa aaaactggcc gataatatcc gtcaggcgca
     1681 tgaaaacgca caagatgtgc acatcagcag ccagaaaatt tccaggcgct tcgcccagat
     1741 cgaaaaggtc gaactggcgc agactgcagg cctggaaacg ctggagttgc tggatgaatt
     1801 agacgaggct ggaaagagtc actgatgatg aagattacca ttttatattt cgcccgtatc
     1861 aaagaggttg tcaattactc ctctgaggat gtcgtgttgc cagagggggt agagacgatt
     1921 gtcgcgctta agtcatggct atccgaccgc ggagatacct ggcaaaagct gtttaacggc
     1981 agtggtgttg tgcgtgcggc cattaaccat gagttggtgg atgatatggc cacatttgac
     2041 gaaggcgatg aagtggcatt ttttccaccc gttaccggag gctgagttga gtgaggcagt
     2101 ctacctgaat gtttccgtac agccggaaga ttttgatacc ggccatgagt tgcaacaact
     2161 cagggcagca acacctgaag caggtgcgct ggtgaatttt gttggtcttg tacgtgaaat
     2221 ctcccagcgt ggacaattaa gccatatgct gattgagcat taccccggta tgactgaaaa
     2281 agcattgcaa cagattgccg ggcaagcggc acaacgctgg ccactattgg gtgtgcgtat
     2341 gatacaccgg gtcggtttgc tcccggcgca aggacaaatt gtacttgtgg cggctaccag
     2401 tttgcatcgg caggccgcat ttgaagcttg cgcctttatg atggattatt taaaaaccga
     2461 agcccccttc tggaaaaagg aagttggtga cttcggtgag cactgggtag aagcccgggc
     2521 atccgatgaa caagcaaaac aacaatggaa actgcctaga tgatacaagt actcagccct
     2581 aatgaaatga ctgtttccta caagctgtcg cccatgacct ttgagtcgac cgaagccttg
     2641 ctggatgcgg ctgatcttga tttgacggat tacggctgga agctgcaacc ggaagcacaa
     2701 aaaacattga tgatgggatt gtcactgcaa caggctggtt gcaatgtgct ggtgctgggg
     2761 acgccagggt ctggccgcgc cggcttgacc ctagccgcca tgaaggccgc cgccgtcact
     2821 ggcatagata gttcgctgac tgatctggtt gcactttacg atttcagcca gcagtcctgt
     2881 gcgcactata ttaaattgcc cgccgggctc ggtgcacagt tgcgtacggt gatggagtct
     2941 tttatcaaag ccctgattgc tgaaatgcag cagtggctgg atgcgagcgg ccaggtaaat
     3001 ttgccacagg ctgagacttg gctggaagag cgtgtggatg gcttgcgctc ccagttgagc
     3061 agcgccaagc tgtcgccttt ctttaatatc atcaaacctg aagtgctggc ttatttgcaa
     3121 gcctggcagc aggtgggtaa tgatggcgaa agtagctcgg acagtattgt caacgatagc
     3181 tttctgtcac gctttcgcgt caacttgctg gtggatcagc gggctaacct gacagccgga
     3241 gtgaagcaac cagtgattga ggagaaagac cccggttttg cagctttgtt tggcatgctg
     3301 gaaaccgcta caggcgagtc tgcccaatgg ccggagttct tgcggttgcg tgccggtagc
     3361 gtgcatcggg ccgatggcgg tatgctgttg ctgcatttgc gcgatttaat gcaggacgag
     3421 ggtaatggca gcgctttgct ggaaaaactc taccgtttca tgcgtaatca cgaagtgcag
     3481 attgaggatt gggcaaacca tgcccagcaa ggtgcgggtg gcaatcgtca tggcacattg
     3541 cctgtgcacg tgaaaatcgt catgattgcc agccgtgagg attattacga ttgcctggag
     3601 gcccagccag acttttttga ttttttcccg atcaaggtcg agtttgaaga tagggtttca
     3661 gccacactgg acaattatgc ttggattgcc ggatacattg cacgcaaatg ccagcagcaa
     3721 gtattgccgc attttagtgg tgctgcagtc aatgtcatgt tgcagtttat gcacagggta
     3781 gaagaggacc aggggcgtat cagtacgcgt tttgcgcatc tggatcaact gatgcatgaa
     3841 agtgtggctg ttgcgacaga tgcacgcctg gtgaccgatg agcatgtcaa gcaggcactt
     3901 gcggccaggt tgttgcgcca acagtttttt gagacccaga tccgtgactc cattattgat
     3961 aatgaattat tgatccaggt gcatgggagc gtcgttggcc aggtcaatgg cctgacccat
     4021 attgagcttg gcgatgccag ctttggctcg cctatccgca ttaccgcccg ctgctatcct
     4081 ggccgtagcg gtgtgatcaa tattgaccgt gaagtcgaaa tgagcggtcc aacgcatgat
     4141 aaagggatct atatcctgca aaactggctg agcgccagct tctgggcatt ggcaccattg
     4201 agcctgaatg cttcactggt gtttgagcag gaatataacg gcgtggaggg cgattctgcc
     4261 tcctgcgccg agttgtttgc gttattgtcc gccttgactg cgctaccaat caagcaaggt
     4321 atggctgtga ctggtgcctt gaatcagttt ggtgaggtga tgcctattgg tggtgtcaac
     4381 gaaaagattg agggttattt ccgtgtttgc caggcactag gcctgaacgg tgatcagggg
     4441 gtgatcatcc ccaaacgtaa ccagcgccac ttgatcctgg atgagagtgt agtaaacgct
     4501 gttaataatg gcatgttcaa gattattgcc attgaccacg tattggaagg cattgcccac
     4561 ctgactgatt gcgaagcagg ggtgcaagag gcagatggta cttatcaagg ggatagcctg
     4621 atggcgcgtg cgcaagcgac cttggcaagt taccgtcgca cgcttgagcg taaccaggca
     4681 cgcatggcag atatccaaca acaagtggag agcggcaagt ctctttaatt agccatggca
     4741 agatcgcaca acaaaagact ggataaaaaa gaacagcctg acgaaaacca ggaagacagc
     4801 tgccgtctgg ataaatggtt atgggcagca cgcttcttta aaacacgcag cctggccgcg
     4861 gaggcagtcg acggcgggaa ggtgcgcatc gatggtgacc gtgccaagaa tgcacgtgaa
     4921 atgaaaccag gcatgcgtgt cagtatcaaa aacaaggaca tgcagattga ggttgaagtg
     4981 ctggccttga gtaatgtcag acgcggtgcg ccagaggctg ctttgctata tcaggaaacc
     5041 gaagaaagcc ttcgcctgcg ccagcaaacc aaagagagtg gggcggatca atatgccgaa
     5101 cgagaccgcg gcatgggtcg cccgaccaag cgccagatgc gcgagattca gcgttttact
     5161 ggccgcggct agcgctttca gctagcttgg gcggctggtg ccagccacta tccccgtttt
     5221 ggccacggct tgtgcgcggc aggacacatc cagtttctgg aaaatacgtt tgaggtggtt
     5281 tttcacggta ttggcgctga tattcaggat catgccaatc tcaagattgg ttttgccact
     5341 tcgcacccaa tgcaggattt cctgttcccg gtcagataag ccgccttggg ccatgctatc
     5401 cagcaagtct tcatctttaa tcaccggtgt caggtgtcca acacggcgga tagcggcatc
     5461 tacatgcggc atgatcatgc ccagcatctg gtcgggcacg tgaaactctt tggtacggtc
     5521 aaaaaagaca tagatgcagt cagctttgcc acgcgtatca cgcacgccat acaccagtaa
     5581 tgaatgcatt ttattcaact gctgggtaaa tgcattaggg gattgtgcgt gaatgccgct
     5641 ggcatcaaag cgattgacga tataccactt atcctcattt tctacccacc gtttgtagag
     5701 gttgcacatc aggtaattga cgatggctga gtcctccagc aggcgttgcg tagtcagctc
     5761 tggaaaagag gaggaaacat caaaatgcag gtcgccattg ctgaagtcgc cccatgcaac
     5821 cagtaataca tcgtgccgca tcagcggatg tatgtcttgc tttagccagt taaagaactc
     5881 aatgtggcgt ttgatcgcat aggagttctg gatggctttc aggatgcctt cccaatagtt
     5941 gtgttttttt tgtgccagca tgaagtcacc tcaaatgatg ctacgcaggg cgcagtttgc
     6001 ccttatatat tcatgatcta agggttttac ggcgtaaatc ggcacttctt taattggaaa
     6061 acagtaataa aacagttgat ttagcgcaag tgcattgaac aatccatcat attctctttt
     6121 caaaaagagt ttgcaattgg
//
LOCUS       DATMSK010000228         5901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_135062,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000228 DATMSK010000000
VERSION     DATMSK010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135062"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="VNZ84_07610"
     CDS             complement(<1..1723)
                     /locus_tag="VNZ84_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HWU66861.1"
                     /translation="MNNSDFLVQAAIYLASAIILVPIFKRLGLGSVLGYLIAGILIGP
                     FALKLIADPEHMLHFAEFGVVLMLFLIGLELESQKVWELRKLLFGLGGMQVTLTIALV
                     TVIAHGLTFSWPEALIIGMGIAMSSTAIGLTALIEKKQLHTQGGQAAFATLLFQDLSV
                     IPLFMVLAFIAPHKTASGFDLWAVTQSIAVIAAIVLASKTILRPIMRIVAQTGIREVF
                     VAFSLLLVIGVSLAMASVGLSMALGTFLAGVLLADSEYRYELRLDIEPVKGLLLGLFF
                     ISVGMTVDLALVANQPMLIFGFALLVVATKLLVLIGLGWFFHYPLRDKLLFGAAISQI
                     GEFAFVIFGVALTQDSISRETYNILNAIVAVSMLVTPILLLLYDRFLMLQCGERPQDA
                     IPENNSVIIAGFGRFGQIVGRVLIAKGITPTLIDNDPNQVDLTREFGWRCYYGDASRL
                     DVLEEAGIAEAKLLVIAVNDAAATLEMAELVKERWPQVKMVVRARSRTDAFDLRDLDL
                     NPVRETFYSSLEAARQALVMIGETSSAAAKIIKQFEKHDIEQLETTRKIRHDRKALSS
                     QMEQGRQD"
     gene            2015..2386
                     /locus_tag="VNZ84_07615"
     CDS             2015..2386
                     /locus_tag="VNZ84_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66862.1"
                     /translation="MLTQNEYAEQIFDVLRFSIDLACCEQNWSNVSRDALAFGFTQTD
                     VSTAFAYAQRFDLFVEPTIFISLHYRSFDPSGPFRALPAVSKFELTMSVGDEVVRAHQ
                     NGFDEPMPSKDPAVWGELSFT"
     gene            2469..3035
                     /locus_tag="VNZ84_07620"
     CDS             2469..3035
                     /locus_tag="VNZ84_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66863.1"
                     /translation="MKRLKQMQYCLIAISSFHASLALSTEPQQLFEPIRSVLQHPRCQ
                     NCHIPGDQPLQLDSGRPHAMLVMRGKDGKGMPAMECTSCHQQKNSPPVMGAHAPPGAP
                     HWQLPPPEHKMVFIDLSPRELCMVLKDPQKNGGRNGEQLIHHFSDDKLVAWGWNPGGN
                     RTVPPLTKAQTVAAVKDWVNAGMPCPNK"
     gene            3063..3236
                     /locus_tag="VNZ84_07625"
     CDS             3063..3236
                     /locus_tag="VNZ84_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66864.1"
                     /translation="MADHVDQTQTTPANPTSPAKVKDKRQPSPNADLEAEIATEVEHG
                     QLTDDELNSGNDI"
     gene            complement(3252..4016)
                     /locus_tag="VNZ84_07630"
     CDS             complement(3252..4016)
                     /locus_tag="VNZ84_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU66865.1"
                     /translation="MRTTSFQSTVYAEPIALQVFGTPAKHKQPVMLFFHGGLFNCGRI
                     DTAHALATALSEQVIVVCVDYPLAPRLHFPETIEVAYQALGWVSQHASSLGADAGRVF
                     VAGEQAGGNLAAVVAMLFRDRGLGCKQRLQLRGQILINPMLDALQTSKSMAAAVDSPC
                     RQAWADYIHVSSHAMHPYVSPVNSCRLANLAPALIMTSESHPLRDEAEAYAAKLLLAG
                     VPVQVRRLEDSKQPLSSAQHPAFTQVCLLSRQFMLA"
     gene            complement(4035..4673)
                     /locus_tag="VNZ84_07635"
     CDS             complement(4035..4673)
                     /locus_tag="VNZ84_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWU66866.1"
                     /translation="MINLYTWATPNGRKISIMLEELGLDYAVSPVDLGKQEQFSAHFS
                     AISPNHKIPAIIDTEPGQEPVVIFESGAILTYLAEKYGRFLAPHGQARYTAFEWLHWQ
                     TSGLGPMLGQLGFFAVRSQEKAPLAIARFTEEADRLLTVMDKQLQTKTYLAGEEYSIA
                     DIASYAWTLAATTYLKDVLKDSLATKPALQRWLKVVGERPAVQRGMQIPKLG"
     gene            complement(4689..5438)
                     /locus_tag="VNZ84_07640"
     CDS             complement(4689..5438)
                     /locus_tag="VNZ84_07640"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWU66867.1"
                     /translation="MSKKLEGKVAVVTGASKGIGAEIARRFARDGASVVVNYLSSKAG
                     ADKVVADITAAGGKAVAVGGNVSEAADAQAIIEAAISHFGKLDILVNNSGIYEFGSIE
                     EITPEHFHKQFNINVLGLLLTTQAAVKYLPEGGSIINIGSVVSRLTPPGAAVYTATKG
                     AVDAITGSLSKELGPRKIRVNALNPGLVATEGTQTAGVIGSEMESGYVAQTPLGRTGQ
                     PNDIASVAAFLASEDSYWLTGEQLLVGGGLR"
     gene            5578..>5901
                     /locus_tag="VNZ84_07645"
     CDS             5578..>5901
                     /locus_tag="VNZ84_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU66868.1"
                     /translation="MDRFDTMLAFTRVVELESFTKAAMSLNLPKTTVSAQVLALEKRL
                     RVKLLHRTTRRISVTTDGAAYYERAIRLLNELKETEAALNQATVAAKGRLRVDVPTPL
                     GRLVII"
BASE COUNT         1458 a         1683 c         1467 g         1293 t
ORIGIN      
        1 aatcctgccg cccttgctcc atttggctgg ataaggcttt gcggtcatgc cggatcttgc
       61 gggtggtttc cagttgctca atatcatgct tttcaaattg cttgatgatt ttggcggcag
      121 cactggaagt ttcgcctatc atcaccaatg cttgccgggc cgcttccagt gaagaataaa
      181 aggtttcgcg tacagggttt aaatccaggt cgcgcagatc aaacgcatcg gttctgcttc
      241 ttgccctgac caccattttc acctgtggcc agcgttcttt caccagttca gccatttcca
      301 gcgtggccgc ggcatcgttg acggcaatca ccaataactt ggcttcggca atcccggctt
      361 cttcgagcac atccagcctg gacgcatcac cgtaataaca acgccagcca aactcgcgtg
      421 tcaggtccac ctggttaggg tcattatcaa tcagggtggg cgtgatgcct ttggcaatca
      481 atacccggcc tacaatttgc ccaaagcggc caaagccggc aatgatgaca ctgttatttt
      541 ccgggatcgc atcctgcggc cgttcgccgc attgcaacat caggaaccgg tcatacagca
      601 atagcaggat aggtgtcacc agcatggaga cagcgacaat cgcattcagg atattgtagg
      661 tttctcgcga aatcgagtcc tgggttaagg ccacaccaaa aatcacaaag gcaaattcac
      721 caatctgtga aatggccgcg ccaaacaaca atttgtcacg caatgggtaa tgaaaaaacc
      781 agcccaggcc aatcagcacc agcagtttgg tagccactac tagcaaggca aagccaaaaa
      841 tcagcatggg ctggttggcg accaaggcca ggtcgacggt catgccgacc gaaataaaga
      901 acaaccccaa tagcaggccc tttaccggtt caatgtctaa cctgagctca taacggtatt
      961 ctgaatctgc cagcagcacc ccggccagaa aagtgcccag ggccatcgac aggccgactg
     1021 aggccatggc cagggagacg ccaatcacca gcaataatga aaaagcgaca aacacttccc
     1081 tgatcccggt ttgggcgacg atgcgcatga tcggccgtaa aatggttttg cttgccagca
     1141 caatcgcggc aatcaccgcg atggactgcg tgaccgccca cagatcaaac ccggaagccg
     1201 ttttatgcgg cgcaataaac gccaacacca tgaacagcgg aatcacggat aaatcctgga
     1261 acagcaaggt cgcaaacgcg gcctggccgc cttgggtgtg taactgcttt ttttcaatca
     1321 gtgccgtcag gccaatcgcc gtagacgaca tcgcaatccc catgccaata atcagtgctt
     1381 caggccaaga aaacgttaag ccatgggcaa tgacggtgac cagtgcaatc gtgagcgtga
     1441 cctgcatgcc tcccaggcca aacagcagct tgcgtaactc ccagactttc tgtgactcca
     1501 gttccagccc gatgaggaac agcatcagca ccacaccaaa ctcggcaaag tgcagcatat
     1561 gctcagggtc ggcgatgagc tttagggcaa aggggcctat cagtatgccg gcaatcaggt
     1621 agcccagcac cgatcccagc cccagccttt taaaaatggg caccagaatg atggcggaag
     1681 caaggtaaat ggctgcctgt actaaaaagt cggagttatt catggatgcc tttaaatgcg
     1741 gatgttgagt gttattggtg acgtttggct ggttaaggct gagttctcgt gaaggttgag
     1801 cctgggccag cacgcctgtt tgatgcagaa agtaccatat cgctgctggc aggtcgagtg
     1861 ctgcgaatgg cggcttggcg cgcttattgc tagcggcttg ccagattaat ctgacaagaa
     1921 aatcagagtt ttgcccgtgt tgccggtggc gtactccacc tacactgtat tcacctactt
     1981 actctctcta aagtttaatc acaggttgcc cttcatgttg acccagaatg aatatgcaga
     2041 acagattttc gacgtattac ggttctctat agacttggcc tgctgcgagc agaactggtc
     2101 caatgttagc cgcgatgcct tggcttttgg tttcacgcaa accgatgttt ccaccgcgtt
     2161 tgcttatgca caacggtttg acctgttcgt agagccgacc attttcatca gcctgcatta
     2221 ccgctccttc gatccttccg gcccgttccg tgccttgccg gcggtaagca agtttgagct
     2281 caccatgtct gtcggcgatg aggtcgtccg cgcacaccaa aatggttttg atgaacccat
     2341 gccaagcaaa gaccccgcag tgtggggtga gctctcgttt acctgatcca aatacgcgac
     2401 tgacctggcc gccatcgcaa tgactggcgg ccagcattca atgactatca ctgcaaggag
     2461 gagccagcat gaaacgtctt aagcaaatgc aatactgcct gatcgccatc agcagttttc
     2521 atgcgtccct ggccctcagc accgaaccac aacagctatt tgaacccatc cgctccgtct
     2581 tgcaacatcc ccgttgccaa aactgccata tccccggcga tcaacccctg caactagaca
     2641 gtggccgtcc gcatgccatg ctggtgatgc gcggcaagga tggcaaaggc atgccagcca
     2701 tggaatgcac ttcttgccac cagcaaaaaa attcgccgcc tgtcatgggc gcgcatgcgc
     2761 cacccggtgc accgcattgg caattaccgc caccagagca taaaatggtc tttatagacc
     2821 tgtctccccg ggagttatgc atggtgctca aagatccgca aaaaaatggc gggcgcaatg
     2881 gcgagcaatt gatacaccac ttttcggatg acaagctggt ggcctggggc tggaaccccg
     2941 gaggcaaccg cacagtgcca ccgttaacca aagcgcaaac ggttgcagcc gtgaaagact
     3001 gggtaaatgc aggcatgcct tgccccaata agtagggctg ttagccatta aggagatgta
     3061 gcatggccga tcatgtggat caaacacaaa caacccccgc caacccaacg tcacctgcca
     3121 aggtcaaaga taaacgccag ccttcgccca atgccgacct ggaagccgag atcgccaccg
     3181 aagtcgagca tggtcagttg acagacgacg agctcaatag cggcaatgat atctaagtcc
     3241 aatcaagcaa tttaagccag cataaactgc ctgctcaaca ggcacacctg cgtaaaggct
     3301 ggatgctgtg ccgagcttaa cggctgcttg ctatcctcca gcctgcgtac ttgcaccggc
     3361 acacccgcca gcaacaattt cgccgcataa gcttcagcct catcgcgcaa agggtgagac
     3421 tcgctggtca tgatcagggc aggcgccagg tttgccaacc ggcaagaatt cacgggtgaa
     3481 acataggggt gcatggcatg actacttaca tgaatatagt ctgcccaggc ttgcctgcaa
     3541 ggcgaatcca ctgctgccgc catcgatttg gacgtttgta gcgcatccag catcgggttg
     3601 atcaggattt gcccgcgcag ctgcaagcgc tgcttacagc ccaagccgcg gtcacgaaac
     3661 aacatcgcca ccaccgctgc caagttgcct cctgcctgtt caccggcaac aaatacacgt
     3721 cccgcatccg cgcccagcga agacgcatgc tggctgaccc aacccagggc ctggtaagcc
     3781 acttctatcg tttccggaaa atgcagcctg ggcgccaacg ggtagtccac acacaccacg
     3841 atcacctgtt cagatagcgc agttgccagc gcatgtgcag tgtctatgcg cccgcagtta
     3901 aacaacccgc catgaaaaaa cagcatgacg ggttgtttat gtttcgcggg tgtgccaaac
     3961 acctgtagcg caatcggttc tgcgtaaaca gtcgattgaa aagaggtggt ccgcatgatg
     4021 gtttccgtat gcccttaacc cagcttgggg atttgcatgc cccgttgcac ggctggccgc
     4081 tcaccaacca cttttaacca gcgttgcaag gcaggcttgg tcgccagtga atctttcaac
     4141 acgtctttca ggtaagtggt ggctgccagt gtccacgcat aactggcaat atcggctatc
     4201 gagtactcct cacccgccag gtaagttttg gtttgcaact gcttgtccat cactgtcagc
     4261 aaacggtcgg cttcttcggt aaagcgggca atcgctaaag gcgctttctc ctggctacgt
     4321 acagcaaaaa atcccagctg gccgagcata gggcccaggc cgctggtttg ccagtgcagc
     4381 cattcaaacg ccgtataacg ggcctggccg tgtggcgcta aaaagcggcc atacttttcg
     4441 gccaggtaag tcaaaatcgc accgctctca aaaatcacca ccggctcctg gccgggctcc
     4501 gtgtctataa tcgccgggat cttgtggtta ggggatatcg cgctgaaatg ggcagagaac
     4561 tgctcctgct tgcccagatc aaccgggctc accgcatagt ccagccccag ctcttcgagc
     4621 atgatggaga tttttcggcc atttggtgtt gcccatgtgt ataggttgat catcgtgtta
     4681 tcacttcatt aacgtaaacc accgcccacc agcaattgct cgccagtcag ccagtatgag
     4741 tcttctgagg ccaggaatgc cgcgaccgag gcaatatcgt tcggttgacc agtacggccc
     4801 agtggcgttt gggccacata gccactctcc atttcagatc caatcacacc agccgtttgc
     4861 gtaccttcag tcgccaccag gccagggttc aaggcattga cacggatttt gcgtgggcct
     4921 aactctttgg atagtgaacc agtgatcgca tctaccgcgc ctttagtggc ggtgtatacg
     4981 gccgcaccag gaggcgttaa acggctgacc acagaaccaa tattgataat gctgccgcct
     5041 tcgggcaggt atttcacggc tgcttgcgtc gtgagcaaca agcccagcac gttaatgttg
     5101 aattgtttgt ggaaatgttc aggcgtgatt tcttcaatgc taccgaattc ataaataccg
     5161 gagttgttga ccaaaatatc cagcttgcca aagtggctaa tcgccgcctc gataatcgct
     5221 tgtgcgtctg cggcctcgct cacattgcca cccacggcta cagccttgcc acctgcggct
     5281 gtaatatccg ccaccacttt gtcggcaccc gctttgctcg ataaataatt gaccaccaca
     5341 gatgcgccat cacgggcaaa acgtctggcg atttcagcac caatcccttt agatgcacca
     5401 gtcacgacgg ccactttacc ttcaagtttc ttgctcataa aaatctccta aataaaaagt
     5461 aaataaagtt gtttgaaact ggtgaccact ataaataaat aaaaaacaca gataaatcac
     5521 ctaaatatgc aatcactgtg ctaatttatt ggacaatcaa gatcaatcag gacgtttatg
     5581 gaccgctttg acaccatgct ggcattcaca agggtggtag agctggagag ctttaccaaa
     5641 gccgccatgt cgctcaattt gcccaaaacc accgtctccg cccaggtgtt ggcgctggaa
     5701 aaacgcctgc gtgtgaagct gctgcaccgc acgaccaggc gcatcagcgt caccaccgat
     5761 ggcgcagcgt attacgagcg ggcgattcga ttactcaacg agctcaaaga aaccgaagcc
     5821 gcgcttaacc aggctaccgt cgctgcaaaa gggcgcttgc gggtagatgt gcccacccca
     5881 ttggggcggt tggtgattat t
//
LOCUS       DATMSK010000229         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_277028,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000229 DATMSK010000000
VERSION     DATMSK010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277028"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="VNZ84_07650"
     CDS             complement(<1..1469)
                     /locus_tag="VNZ84_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWU66869.1"
                     /translation="MQAETNLLQGLNDKQLQAVTLPHVSALILAGAGSGKTRVLTTRI
                     AWLIQTGQTSPMGLMAVTFTNKAAKEMLTRLTSMLPINTRGMWAGTFHGLCNRMLRAH
                     YREAGLPNTFQILDTADQLSAIKRIMKAMNVDDEKYPPKQVMGYINSCKEEGLRAEAV
                     DAYDGHSQKLREIYAAYDQQCNREGVADFAELLLRCYELLEREPHIRAHYQQRFQYIL
                     VDEFQDTNRLQYLWLKLLGGAHNSIFAVGDDDQSIYAFRGARVGNMNDFQRDFNVQNI
                     VKLEENYRSHSNILDAANAIIANNRNRLGKNLWTSAGKGEPIRIYEAYNDTDEAQFIV
                     DEVNMLQKEGVPLGDMALLYRSNAQSRILEHTLFSANIPYRVYGGLRFFERAEIKHAL
                     AYMRLIANANDDTALLRIINFPPRGIGARGLEQLQEAARQNECSLWQAATTKVGEGKL
                     GSKGLDGFVALIRQMVDQGYGITLGEMAEMVINLSGLMA"
     gene            complement(1691..>2017)
                     /locus_tag="VNZ84_07655"
     CDS             complement(1691..>2017)
                     /locus_tag="VNZ84_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HWU66870.1"
                     /translation="AGLDYHEYAELTVYENWDECRQALHDKRMFALTTKGSTRHTDLQ
                     FKDEDVFVFGPESRGLPEDIRAQFPPEHRVRLPMLPHSRSLNLSNSAAILLYEAWRQT
                     GFSGGV"
BASE COUNT          440 a          529 c          521 g          527 t
ORIGIN      
        1 tgcgccatca agccactcag gttaatgacc atctcagcca tttcgcctaa ggtgatgcca
       61 tacccttggt ccaccatctg ccggataagc gccacaaagc catccaggcc tttgctgccc
      121 agcttacctt caccaacttt ggtagtggcg gcttgccaca gactgcattc gttttggcgg
      181 gcggcttcct gcaattgttc caggccgcgc gcaccgatac cacgtggcgg gaagttgatg
      241 atgcgcagca aagcggtatc atcattggcg ttggcaatca gccgcatata ggccagggcg
      301 tgcttaattt cagcacgctc gaaaaagcgc aggccaccat aaacgcgata cgggatatta
      361 gccgaaaaca gggtgtgctc aagaatccgt gactgcgcat tgctgcggta tagcagggcc
      421 atatcgccca atggcacgcc ttctttttgc agcatgttca cttcgtcgac aataaactgc
      481 gcttcgtcgg tatcgttata cgcttcataa atccggatcg gctcaccctt gccagcggat
      541 gtccacagat tcttgcccaa gcggttgcgg ttgttggcaa taatcgcatt ggcagcatcc
      601 agaatgttgc tatgcgaacg gtagttttct tcgagcttga cgatattctg cacattaaag
      661 tctcgctgga aatcattcat gttgccgacg cgcgcgccac ggaaagcata aatgctctgg
      721 tcgtcatcac ccacggcaaa aatactgtta tgggcgccac ccagcaattt cagccacagg
      781 tattgcagac gattggtgtc ttgaaactca tcgaccaata tgtattgaaa gcgctgctgg
      841 tagtgagcac ggatatgtgg ctcacgttcc agcaattcgt aacagcgcag caataactcg
      901 gcaaaatcag ccaccccttc acggttgcac tgctggtcat aggccgcata aatttcgcgt
      961 aatttctggc tgtgtccatc gtacgcatct acagcttcgg cacgcaggcc ttcttctttg
     1021 caactattga tatagcccat cacctgtttg ggtggatatt tttcatcatc cacattcatg
     1081 gctttcatga tgcgtttgat ggcagacagc tggtcagccg tatccagaat ctggaaggta
     1141 ttcggcaagc ccgcttctcg gtaatgtgca cgcagcatgc ggttgcataa gccgtggaaa
     1201 gtgcctgccc acatgccgcg ggtattgatt ggcagcatgc tggtcaggcg tgtcagcatt
     1261 tccttggcgg ccttgttggt aaaggtcacg gccatcaggc ccatggggga ggtttgtccg
     1321 gtctggatta gccaggcaat gcgtgtggtc agcacccggg ttttgccact cccggcaccc
     1381 gccagaatca atgcagagac atgcggcagg gtgaccgctt gcagttgttt gtcgtttaaa
     1441 ccctgtaata aattggtttc agcttgcatg tcttattatc gcatgacgtt tctactgggt
     1501 ggagtgataa atcttcactg cctgaaggtg ggagttaagt tggtggaact ttttaaacag
     1561 gccattgctc ttaccaatac gatgatgatg aatcatcttt ccgcttcttt cctccctgag
     1621 cggaagcctt tacgatgagg cctgttgccc cgatttaccc cttaatcggg gctttttttg
     1681 tctattggtt ttacacccca cccgaaaagc cggtttggcg ccaagcctca tacagcaaaa
     1741 tggccgccga gtttgacagg ttcagactgc ggctgtgtgg cagcatgggt aatctgacgc
     1801 gatgttctgg cgggaattgg gcacggatgt cttctggcag gccgcgagac tccggaccga
     1861 atacaaatac atcttcatcc ttaaattgca agtcagtgtg gcgtgtgctg cctttggtag
     1921 tgagtgcaaa catgcgttta tcgtgcagcg cctgcctgca ttcgtcccag ttttcataga
     1981 cagtgagctc ggcgtattca tgatagtcca gcccggc
//
LOCUS       DATMSK010000230         7885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_323914,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000230 DATMSK010000000
VERSION     DATMSK010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323914"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VNZ84_07660"
     CDS             <1..704
                     /locus_tag="VNZ84_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU66871.1"
                     /translation="LPEMRPLYKEFTVDEYLTIAARLHNVSSKHIKKAVEHAKERCGL
                     THMSKRLIENLSNGYQQRVGIAQAIIHNPMVVILDEPTVGLDPIQIRDIRALIKEIGQ
                     AHSVIVSTHILPEVEMVCDHVQIIDKGKLVFNGGIDVLKQQRVGNKLLVGLRNAPEAA
                     QLAAIAGVAEVESADGMWRIRFQDGTQPHEAIVQAAVQHGWGLYHIAPDQTSLEDVFV
                     QLTYHDANTEDAKAA"
     gene            726..1469
                     /locus_tag="VNZ84_07665"
     CDS             726..1469
                     /locus_tag="VNZ84_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWU66872.1"
                     /translation="MLNIARKELKSMFASPMGWIILALLTFSFGTYYLNGVNNYFEVM
                     SGSIRPAERIGVTQFVGQTVYGLASFIMLFAVPLLSMRLISEERRSQTMPFLFSAPLS
                     ISEIVVGKFLGLVVFLSILVVYIAAMLSTLNIWADIDFGYIFANSLGLLLLVSSFAAL
                     GLYFSSLTAQPIIAGILSFIALFVLMILDRFFAGDPTSTVLKFSLMRHFQSFAGGLID
                     TADLAYFGLFILTFITLTIRRLDADRLRG"
     gene            1500..2894
                     /locus_tag="VNZ84_07670"
     CDS             1500..2894
                     /locus_tag="VNZ84_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HWU66873.1"
                     /translation="MKINRKFRWQLLAQNWMFVLVFLALVVMLGYLSSQYRWAKDITQ
                     ANRNILTQGSVNVLKQMDGDINITVYATNDDANNGDTFRKGMLDFVARYQREKKNVFI
                     KFINPSEEPKMAQEAGVKVDGETIVEYNKRSEHITPPFAEQEMTNLLVRLSRTNNQPV
                     MYVDGHGERLLQGLKNHDLGEFGKQLESKGFKFANPDLTIAQAVPANGAMLVIAAPQV
                     DISAVEAKKIKAYIEAGGNVLWLLDDDNLHGLQEVADYLGIKVSPGIVVDRAAEQYGA
                     DPKMAFASQYGDHAITRNFMLRTVFPEAHQIDGQASDDLGWKVGRLIDVAPNGWLESD
                     KIPAGAQNLKVSFDQGKDKAGPINVGVALEREYGKKGQRVVVIGNANFLANTFITNGG
                     NLDLGINIINWLAGDDRLITIQPMPLKDINVTIPATDQGRLVAWSVFHAFQYLIPVGL
                     FVAGLFLWWKRRKA"
     gene            2920..3825
                     /locus_tag="VNZ84_07675"
     CDS             2920..3825
                     /locus_tag="VNZ84_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWU66874.1"
                     /translation="MKKRWILNLLLLAVVVGIAAFLHLKPQEQSQSAKFEVSSLKMAD
                     FDAVKAEFPAKAPTVFEKQDGYWMMRKPYAARADQPSVQRIISIIAATTATKLPLQDA
                     TKYGLDQPVLKLALSGTKGDQVFTFGTYNPVTEEQYVGFAGQVYLLPGQYSEAASTQP
                     IEMVDKTPLSPTERKQLAGFDLAHLEQWEENALKVTLSTEGKWSVNDPNAKPTQNDMN
                     EWTDFSWVQAQAVSVEVYTPDRKQAYPSFEVLLRNGKKIHFDKMQESPEYLLARPDEG
                     IIYHFSNDSGFTMVNPPVNIQTPKK"
     gene            3828..4679
                     /gene="mutM"
                     /locus_tag="VNZ84_07680"
     CDS             3828..4679
                     /gene="mutM"
                     /locus_tag="VNZ84_07680"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWU66875.1"
                     /translation="MPELPEVEVTRLGLLPVEGAFIEQVTIRHHGLRWPIPQDLPSRL
                     QGRRILKLKRRAKYILAEVGTAEVEGVLLLHLGMSGRLCLLERDFPAEKHDHFDIRFA
                     DGRVIRLRDPRRFGAVLWLDQDPMQHALLKHLGPEPLEPAFNADYLYQQLRTRSAAIK
                     PTIMDAHLVVGVGNIYASESLFRARIHPETPAKDLTQAQCKLLVEEIKLTLSAALEAG
                     GSSLRDFFGVDGNPGYFQQTYFVYGRTGEPCRVCQQAIVTIRLGQRSTFYCQVCQPRM
                     SIRPGKK"
     gene            complement(4693..6153)
                     /locus_tag="VNZ84_07685"
     CDS             complement(4693..6153)
                     /locus_tag="VNZ84_07685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWU66876.1"
                     /translation="MVVNKRYYTLLLSAIVLIGFVATSLIGYFVAKDSLTERLQQEML
                     PLTSDNIYSEIQRDLLQPLLISSLMANDVFVFDWVNDGEQDASRMTSYLAQIQKKYNT
                     ITAFFVSESSGNYYHPSGVLKRVSTDNPDDHWYFDTKNSHNPYVINIDRDTADKRRLS
                     IFVNYRIEDKSGKFIGVIGVGLSLQTVVELIENYQKRYGREIYFVNRQGEVMLQSSHY
                     RSELHIQNKSGLDKAFLKVLTSPSASFSFKTSSGNMLYLNSRLVPEFDWYLIVEQVND
                     PSSERIETAFFINLAVAVVISMLVLALAHFAVRGYHDKLEMMATQDKLTGVASRQVFE
                     FYFKQAVARSKRRDEAVSLVLLDIDLFKQINDSYGHQAGDLVLTRVAQLIKSHIREED
                     VVCRWGGEEFLLLLSGCNLQYARDITELIRSAVAGLQFHFNNKIVHITVSAGIAEMQT
                     NETLGQVVERADHHLYAAKRAGRNCIRPDSDDEEQA"
     gene            complement(6284..7753)
                     /locus_tag="VNZ84_07690"
     CDS             complement(6284..7753)
                     /locus_tag="VNZ84_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HWU66877.1"
                     /translation="MLIWFVVAYLLVSIGIGLIAARNVHSTKDYAVAGRHLPLPVVMA
                     TVFATWFGAEAVFGVSATFVREGLTGVAADPFGSSMCLIIAGIFFSSRLYKLNILTLG
                     DFYRMRYNRSVEVLTTIAIVISYMGWVAAQIKALGLIFNLISHGAISEQAGMILGTAI
                     VLTYTTLGGMLSVAILDFVQMIVVIGGLLYIANIVSGQTGGVAPVIEHARNAGKLHFF
                     PQSTDWSLWLTFLGGWLTMMLGSIPQQDVFQRITSAKTANIALWGSIWGASIYFCFTF
                     VPMFIAYAATLIDPATFGTLVTTDSQRVLPTLVLTHTPLLAQAIFFGAVLSAIMSCSS
                     ATLLAPSVTFAENVVKGFMPHMNDYAFLRIMRLCLVGFAVMVLLYALNSELSIFGMVE
                     SAYKVTLAGAFVPLFFGAFWKRATSQGALAAIIFGIASWVLIEVMIGEESLIPAQLVG
                     LLVSAAAMVIGSLLPQKIGGTPHSPTGYLHEHAARIHPH"
BASE COUNT         1948 a         1892 c         2113 g         1932 t
ORIGIN      
        1 acctgccgga aatgcgtcct ttatataaag aattcactgt tgatgaatac ctgaccattg
       61 ctgcgcgttt gcacaatgtg tccagcaagc atattaagaa agcagtcgag catgccaaag
      121 aacgttgtgg cctgacacac atgagcaagc ggctgattga aaacctgtcc aatggttacc
      181 agcagcgtgt gggcattgca caagccatta ttcacaaccc tatggtcgtg attctggatg
      241 agccgaccgt cggcctagac ccgatccaga ttcgtgatat tcgtgccttg attaaagaaa
      301 tcggccaggc gcacagcgtc attgtctcta cccacatttt gcctgaagtg gaaatggtgt
      361 gtgaccatgt acagattatc gataagggca aactggtatt caacggtggc attgatgtgc
      421 tcaagcagca gcgtgtagga aacaagttgc tggttggttt gcgcaacgcc ccagaggcgg
      481 cacaactggc agcgatcgcc ggggttgccg aagtggaatc ggccgacggg atgtggcgca
      541 tccgcttcca ggacggcacg caaccccatg aagcgattgt gcaggcggca gtgcaacacg
      601 gctggggcct gtatcatatc gcccctgacc agaccagcct ggaagatgtg tttgtgcaac
      661 tgacttacca tgatgcaaac actgaagatg ccaaagcggc ctaagcggat acggaaaaac
      721 taagtatgtt gaatattgca agaaaagaac tgaagagtat gttcgcctca ccaatggggt
      781 ggattattct ggcattgctg accttctcgt tcggtaccta ttacctcaac ggggtcaata
      841 attattttga agtcatgtcc ggctccatcc gccccgcaga gcgtatcggc gtcacacagt
      901 ttgttggtca aaccgtgtat ggcctggcct catttatcat gttgtttgcc gtgcctttac
      961 tctccatgcg cctgatttca gaagagcgcc gcagccagac catgccgttt ttgttcagtg
     1021 caccgttatc tatcagtgag attgtggtcg gcaagtttct gggcctggtg gtgtttttga
     1081 gtattctagt ggtgtatatc gccgccatgc tgtctacgct caatatttgg gctgatatcg
     1141 attttggcta tatttttgcc aactcactgg gactgctgct actggtgtcc agtttcgccg
     1201 ccctgggcct gtatttctcc agcctgacgg cacagcctat tattgccggt atcctgagtt
     1261 ttattgcctt gtttgtactg atgatcctgg accgcttctt tgctggtgac ccgaccagca
     1321 ccgtgctcaa gttttccttg atgcgtcact tccagtcgtt tgccggtggc ttgattgata
     1381 cggctgacct ggcttatttt ggcctgttta tcctcacttt tattaccctg acgattcgtc
     1441 gtctggatgc agatcgtttg cgcggttaaa cgcggcttgt ttttgagttt gaaagcatta
     1501 tgaaaattaa ccgtaagttt agatggcaat tgttggcaca aaactggatg tttgtgctgg
     1561 tgtttctggc gctggtggtg atgttagggt atttgtccag ccaataccgc tgggccaagg
     1621 atattaccca agctaaccgt aatatcctga cccagggcag cgtcaatgtg ctcaaacaga
     1681 tggatggcga catcaatatt accgtgtatg ccaccaacga tgatgccaac aatggtgaca
     1741 ccttccgcaa aggcatgctg gactttgtgg cgcgctatca gcgtgaaaag aaaaacgtgt
     1801 ttatcaagtt catcaacccg tctgaagagc caaaaatggc gcaagaagct ggggtgaagg
     1861 tggatggtga aaccattgtt gaatataaca agcgttccga gcacatcacg ccaccgtttg
     1921 ccgagcagga gatgactaac ttgctggtac gtttatcccg taccaataac cagccggtga
     1981 tgtatgtgga tggtcatggc gagcgtttgc tgcagggcct gaaaaaccat gacctgggtg
     2041 agtttggcaa acagcttgaa agcaaaggtt ttaaatttgc caatccggac ctgacgattg
     2101 cccaagcggt gccagccaat ggcgccatgc tggtgattgc cgcgccacaa gtggatatca
     2161 gcgcggtaga ggcgaaaaaa atcaaagcct atattgaggc cggcgggaac gtattgtggt
     2221 tgctggatga tgacaatttg catggcttgc aagaggtcgc tgattatctg gggatcaaag
     2281 tttcacctgg catagtggtt gaccgtgcgg ccgaacaata tggcgctgac ccgaaaatgg
     2341 cgtttgccag ccaatatggc gaccatgcga ttactcgcaa tttcatgttg cgtaccgtat
     2401 tccctgaagc acatcagata gacggacagg catctgatga cctgggctgg aaagtgggcc
     2461 ggctgattga tgtcgccccc aatggctggc tggaatccga caaaatcccg gctggtgcac
     2521 aaaacctgaa ggtgagtttt gaccagggca aggataaagc cgggccgatt aacgtcgggg
     2581 tggcattaga gcgtgagtac ggtaaaaagg gccaacgggt agtggtgatt ggcaacgcta
     2641 atttcctggc caatactttc attaccaatg gtggtaactt ggacttgggt atcaacatta
     2701 ttaactggtt ggccggagat gatcgcctga ttaccataca gccgatgccg ctcaaggata
     2761 tcaacgtgac gattcctgcg acagaccaag gtcgcctggt agcctggagt gtgtttcatg
     2821 ctttccagta tttgattccg gtcggcttgt ttgtcgcggg cctgttcctg tggtggaagc
     2881 gccgcaaggc ttaatggcta gaatccttag gatgttgtta tgaaaaaaag atggattctg
     2941 aatctgttgt tgctggccgt ggtggtgggc attgctgcgt tcctgcattt aaaacctcaa
     3001 gaacagtccc agtcggccaa atttgaggtg tccagcctga agatggccga ttttgatgcg
     3061 gtgaaagccg agttcccggc caaggcgcct accgtgttcg agaagcagga tggctactgg
     3121 atgatgcgca agccatatgc tgcacgtgcg gaccagccgt ctgtgcaacg catcatctca
     3181 attatcgctg cgactactgc gaccaagcta ccattacagg atgccaccaa gtacgggctg
     3241 gaccagcccg tgttaaagct cgccttgtcc ggtaccaaag gggatcaggt atttaccttc
     3301 ggcacttaca atccagtgac tgaagagcag tatgttggct ttgccgggca ggtgtatttg
     3361 ttaccaggac aatacagtga agcggcttct acacagccga ttgaaatggt ggataaaaca
     3421 cctttgtcac caacagaacg caagcaattg gcaggctttg acctggcgca tcttgaacag
     3481 tgggaagaaa acgcactcaa ggttacgttg tccactgagg gtaaatggtc tgtgaatgac
     3541 ccgaatgcga agcctaccca gaatgatatg aacgagtgga cagattttag ctgggtgcag
     3601 gcacaagccg tttcggtaga ggtttatacc cctgaccgca aacaggctta cccttcgttt
     3661 gaagtgttgt tgcgtaatgg caaaaaaata cattttgaca agatgcagga gtcgccggaa
     3721 tatctgctgg cacgtcctga cgaaggtatt atctatcact tcagtaatga cagcggattt
     3781 accatggtca atccgccggt gaatatccag acaccgaaaa aatagacatg ccagaattac
     3841 ccgaggttga ggttacgcgt ctagggttgc tgcctgttga gggcgcattc attgaacagg
     3901 tgaccatccg ccatcatggc ttgcgttggc ccattccaca agacttgcca agtcgtttgc
     3961 aggggcgccg cattctcaag ctcaaacgcc gtgccaagta catccttgcc gaagtgggca
     4021 ctgctgaggt tgagggcgtc ttgctactgc acttaggcat gtctggccga ttgtgtttgc
     4081 tggagcggga tttcccggcc gaaaagcatg atcattttga tatccgtttt gccgacggac
     4141 gcgtcattcg gttgcgcgac cccaggcgat ttggcgcggt cctgtggctg gatcaggatc
     4201 ccatgcaaca tgccctgctt aagcatttgg ggccagaacc gctggagcct gcttttaatg
     4261 cggattattt gtaccagcaa ctgcgtaccc gcagtgccgc catcaagccc accattatgg
     4321 atgcccactt ggttgtaggc gtagggaata tttatgccag tgaatcctta ttccgtgccc
     4381 gtatccaccc ggaaacgcct gctaaagatt taacccaggc gcaatgcaag ttgctggtag
     4441 aggagatcaa gttgactctc agtgctgcgt tggaggcggg ggggagtagc ttgcgggact
     4501 tctttggtgt agatggtaat cctggttact tccagcaaac ttatttcgtt tatggtcgta
     4561 ccggagagcc ctgccgtgtt tgccagcaag ccatagtcac aatacggctt gggcagcgct
     4621 ccaccttcta ttgccaggtt tgccagccgc gtatgtccat ccgccctggc aagaaatagt
     4681 tacaaccgtt ttttacgcct gttcttcgtc gtctgagtct ggcctgatgc aattacgtcc
     4741 tgcacgtttt gcggcgtata agtgatggtc tgcacgctca accacctggc ccagggtctc
     4801 attggtctgc atttcggcaa tcccagcact gaccgtgatg tgtacgatct tgttgttgaa
     4861 gtggaattgc aagccggcga cggctgagcg gatcagctca gtaatgtctc tggcatattg
     4921 gagattgcag ccagacagca gcagtaaaaa ctcttctcca ccccagcggc agaccacatc
     4981 ctcttcccga atgtgggatt taatcagttg agccacccgc gtcaacacaa ggtcgccagc
     5041 ctggtgacca taagagtcgt tgatctgttt gaacaggtca atatcaagca agaccaggga
     5101 caccgcttcg tcacgacgtt tgctgcgggc aaccgcttgc ttgaagtaaa actcgaagac
     5161 ctggcggctg gcgacgcccg tgagtttgtc ctgggtcgcc atcatttcca atttatcgtg
     5221 atagccacgt acagcaaagt gtgccagtgc caggacgagc atgctaatca ccactgcaac
     5281 ggccaggttg ataaagaagg cggtttcgat acgttccgag ctcgggtcgt tgacctgttc
     5341 tacgatcaga taccagtcaa attcagggac gagccggctg ttgaggtaca gcatattgcc
     5401 actgctggtt ttaaacgaga aagaagcact cggtgaggtt agcaccttga ggaaggcttt
     5461 atccaggcca gatttgtttt gtatatgcaa ttcagagcgg taatggctgc tttgcagcat
     5521 gacttcaccc tggcggttca caaagtagat ttcgcggcca taacgtttct ggtagttctc
     5581 gatcagctcc acaactgttt gcagggataa gcctaccccg atcacaccaa taaatttgcc
     5641 ggacttatct tcgatacggt agttgacgaa aatactgagc cgtcgcttgt cggcggtatc
     5701 ccggtcaata ttgatgacat agggattgtg cgaattcttg gtgtcaaaat accagtgatc
     5761 atcagggttg tccgtggaaa cccgttttaa tacgccgctg ggatggtagt agttacccga
     5821 gctttcagag acaaaaaacg cggtaatggt gttatatttt ttctggattt gtgccaggta
     5881 gctggtcatc ctgctcgcat cttgttcgcc atcattgacc cagtcaaata caaatacatc
     5941 attggccatc agggacgaaa tcagcagggg ttgcaacagg tcgcgctgaa tttccgagta
     6001 aatgttgtca ctggtgagcg gtagcatttc ctgttgcaaa cgctcagtga gggagtcttt
     6061 ggcgacaaag taaccgatga ggctggtcgc aacaaagcca atgagcacaa ttgcgctgag
     6121 gagtagagtg tagtaacgct tgttaacgac cataaatatt caattatcgc aagacagaag
     6181 attccagaaa agcattatca tcaatataag atgactttgt ttacttgaat agttgctatt
     6241 ttggagatgt attccactaa ttgtctgatg agtgcggtca gatttaatgc ggatgaatac
     6301 gtgccgcgtg ctcgtgtaaa taaccggtgg ggctgtgagg tgtcccacct atcttttggg
     6361 gtagcaatga gccgatgacc attgcggcag cgctgaccag caatcctacg agttgtgccg
     6421 ggatcaggct ttcttcgcct atcatgactt caatcagcac ccagctggca atcccgaaaa
     6481 taatcgctgc caatgcgccc tggctggtgg cccgtttcca gaatgcacca aaaaataagg
     6541 ggacgaatgc gcctgccaga gtgactttat atgcactttc gaccatgcca aaaatagaca
     6601 gttcggagtt gagtgcatag agcagcacca tgacggcaaa gccgaccagg cacagacgca
     6661 tgatgcgcag gaaggcatag tcattcatat ggggcatgaa acctttgacg acgttttcag
     6721 caaaagtcac cgaaggagcc aatagggtgg ctgaggagca gctcataatc gcagacagta
     6781 cagcaccaaa gaaaatggct tgtgctaaca gtggcgtatg tgtgagtacc agcgtgggca
     6841 atacgcgctg ggagtctgtg gtgaccagtg taccaaaggt ggccgggtca atcagggtcg
     6901 ccgcataggc aataaacatg gggacaaaag taaagcaaaa ataaatgctc gcgccccaaa
     6961 tagagcccca caaggcaata ttggccgttt ttgccgaggt aatccgctgg aatacatcct
     7021 gttgcggaat agagcccagc atcatggtga gccagccgcc taaaaaggtt agccacaggc
     7081 tccagtcggt tgattgggga aagaaatgca gtttgccggc attgcgcgcg tgttcaatga
     7141 ccggggctac cccgccagtc tggcctgaaa cgatattggc gatatatagc aagccaccga
     7201 tcaccacgat catttgtaca aagtcgagga tggcgacaga cagcatgcca cccaaggtgg
     7261 tatacgtcag gacaatggcc gtccccagga tcatgcccgc ctgttccgaa attgcgccat
     7321 gggagatcag gttgaaaatc agccccagcg ctttaatttg tgcggcaacc cagcccatgt
     7381 aggagatgac aatggcaatg gtggtgagta cctctaccga acggttgtag cgcatgcggt
     7441 agaagtcgcc cagcgtcagg atgttaagtt tgtagaggcg gctggaaaag aaaatgcctg
     7501 caatgatcaa acacatactg gaaccaaacg gatccgcagc aaccccggtc aagccttcac
     7561 gcacaaaggt ggcggaaacg ccaaataccg cttctgcccc aaaccaggtg gcaaaaacgg
     7621 tcgccatgac cacagggaga ggcaggtgac ggccagccac ggcataatct ttggtgctgt
     7681 gcacattcct ggccgcaatc aggccgatgc cgattgaaac cagtaaatac gctacgacaa
     7741 accaaatcag cacagcccgt ttcctttgat cgattgcaaa aggccgattt tacaacaaaa
     7801 ccatgcttaa acatggtttt ctcgctgggc tggagagtaa aaaggcctga aaatcaggcc
     7861 tttttacaat tctgcacaat tttat
//
LOCUS       DATMSK010000231         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_440695,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000231 DATMSK010000000
VERSION     DATMSK010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440695"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VNZ84_07695"
     CDS             <1..389
                     /locus_tag="VNZ84_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66878.1"
                     /translation="MQNMNKLLIALLVSVISTASSYAAESKDDAATLGTSKSPQVDRQ
                     QSRTDKGPIGGYQDSVNHDASNLGNADNPQVKDQSQRTDKGSADRPFKRHKNKVLRNK
                     DEKAGTTQGNPVQPTEYEAAPPATTN"
     gene            560..928
                     /locus_tag="VNZ84_07700"
     CDS             560..928
                     /locus_tag="VNZ84_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66879.1"
                     /translation="MSLAINRKLILTAIICLLLYQVGTWITIVAGAVWGAGAAVVVAV
                     VSYVCARLAKRSATSTIWFLIPTLLFTVIPLLAKAWSLFTSQTGIVDTLIDLVPLLVG
                     FVIPVGLLSTVYLDLRKKTF"
     gene            1019..3007
                     /locus_tag="VNZ84_07705"
     CDS             1019..3007
                     /locus_tag="VNZ84_07705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HWU66880.1"
                     /translation="MQSSYFGKPRSSSKLWAIGHWFGPYAPLIVMFISGLVFLSLTRL
                     GLVVWKWERVQATGELCQILLQGVRVDVIQLGLLAIIPVLLSPVLATRKFFNVWRVFA
                     FWWVLLSLVLLVFLEAATPGFILEYDVRPNRIFVEYLKYPHEVFGMLWQGFKPELFFG
                     LLMTLIAFALVARLLKPWREAEAKWASPAVWLVWPLILMLTVFGIRSTLTHRPANPAL
                     FAITQDSMVNSLILNSTYSVFYAAYSLQHEAKSSAIYGKMPREKIFALTSAKDGDIPT
                     LTTLVPSVKRDKPLNLVILLQESLGAGFVESLGGKPVTPNLEKLKDEGIWFTHLYATG
                     TRSVRGIEAVVTGFQPTPSDSTVKLDLSQKNFFTLAALLSKRGYLTEFIYGGESHFDN
                     MRSFFMGNGFQQITDQPDFKNPVFVASWGASDEDLLNKTHDQLMAHHASGKPFFTLAF
                     SSSNHAPFEFPDGRITLYEQPKATDNNAVKYADYAIGEFIKKAKASPYWNDTVFLIVA
                     DHDIRVRGDSLVPIEHFHIPALILGGAVQPKTVDAIASQIDLPVTVLSLMGIEAQHPM
                     TGHDLSNLGADYRGRAMMQYNYNFGWMQQKGTDAAENEVVVLREGKPPAFGHYDPVSK
                     QLQNTPAPADANDLAERALANSLLPALLYKEQRYRLPE"
     gene            3016..>3593
                     /locus_tag="VNZ84_07710"
     CDS             3016..>3593
                     /locus_tag="VNZ84_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66881.1"
                     /translation="MKKPQPSMLTRQVIIDGLKYIFSGRLWLKNSHLPAINHTVPEDF
                     TGLCVASNADPATDSYVLEQLLALGVKRVRLDISNDEARPFQERFLRQLLANGFSVTV
                     HAVQPYEDAKNMRSSQAQAGWKQFIEELCQAYAGSIAALEIGNTVNRKKWAGYDMPGF
                     MAAWQIAYDLARQYQVTVIGPNIQDFEPLYNI"
BASE COUNT          843 a          857 c          961 g          932 t
ORIGIN      
        1 ggatgcaaaa catgaataaa ctactgattg cattactcgt ttctgtgatt tcaacggcgt
       61 cttcttatgc ggcggaaagc aaagatgatg ctgcaacgtt agggacttct aaaagtcctc
      121 aagtggatag gcagcagagc cgtactgaca agggccccat tggtggatac caggatagcg
      181 tcaatcatga tgctagcaat ctgggtaatg cggataaccc gcaagttaag gatcagagcc
      241 aacgtacgga caaaggctcc gcagaccggc cctttaaaag acacaaaaac aaggttttaa
      301 gaaacaagga tgaaaaagcg ggaaccaccc aaggaaaccc cgtacagccg acagagtatg
      361 aggctgcgcc tccggcaaca accaattaag gttcatcttg ttttgaggtg ggattgctga
      421 tgcaatccca tttttatgcg ttgagatcca atgaatagtt gccccttcac tcagtaaatg
      481 atgtttaacc atttctggta accagcacct ttaagagtaa aggtggtctg acggattcct
      541 aataaaaaga gttttgtcta tgtcactcgc catcaatcga aaactgattc tgactgccat
      601 tatctgctta ttgctttacc aggtaggtac ctggatcacc attgtcgcgg gtgcggtatg
      661 gggtgcaggg gccgcagttg ttgtcgccgt ggtttcgtat gtctgcgcac gcctggccaa
      721 acgtagcgcc acaagtacga tctggttttt gatcccgacc ttgctgttca cggtcattcc
      781 tttactggcg aaagcgtgga gtttatttac cagccagacg gggattgtcg ataccctgat
      841 cgatttagtg cctctgctag tgggctttgt catcccggtc ggtttgttat ccacggttta
      901 tctggattta cgcaagaaaa cgttttagcc aggcgtaggc ctggcgacag agcgctttga
      961 atcacaatct gcaataaccg ttataatgac aggcttgact acccctgatt aagcaaacat
     1021 gcaatccagt tatttcggta agccgcgttc ctcctccaag ttatgggcca ttggtcattg
     1081 gtttggccct tatgcaccgc tgatcgtcat gttcatcagt gggctggtgt tcctctcact
     1141 gacacgcctg ggtttggtgg tgtggaaatg ggaacgggta caagccacgg gggagctgtg
     1201 tcagatttta ctgcaagggg ttcgcgttga tgtgatccag ctcggtttgc tggcaattat
     1261 cccggtgttg ttgtccccag tgctggccac gcgcaagttt ttcaacgtgt ggcgcgtttt
     1321 cgcgttctgg tgggtgttgc tgtcactggt gctgctggtg tttcttgaag ctgccacgcc
     1381 gggatttatt cttgagtatg acgtgcggcc aaaccgtatt tttgttgaat acctcaagta
     1441 tccacatgag gtgtttggca tgctgtggca aggcttcaag ccggagctgt tttttggtct
     1501 gctgatgact ctgattgcgt ttgcactggt ggctagatta ctcaagccct ggcgcgaggc
     1561 ggaggcaaaa tgggccagtc cggccgtgtg gctggtatgg ccgctgatac tgatgttaac
     1621 ggtatttggc attcgctcca cgctcacgca tcgtcctgcc aatcctgccc tgtttgccat
     1681 tacacaagac agcatggtca acagcctgat cctcaactcc acttattcgg tgttctatgc
     1741 cgcttacagt ttgcagcatg aagccaagtc tagcgcgatt tatggcaaga tgccgcgcga
     1801 aaaaatcttt gccttaacca gcgcaaaaga cggtgacatt cctacgctga cgaccctggt
     1861 accgtccgtt aaacgtgata aaccgcttaa cctggtgatc ctgctgcaag aaagtttggg
     1921 cgcaggcttt gtagagtcac tgggtggcaa accggtgaca cctaacctgg agaagttgaa
     1981 agacgagggt atctggttta cccatttgta tgccacgggt acgcgttcgg tacgtgggat
     2041 tgaagcggta gtgacagggt ttcagccgac gccatccgat agcacggtca agctggattt
     2101 aagccagaaa aactttttta cgcttgcggc tttattatct aaacgtggtt acctgaccga
     2161 gtttatttat ggtggtgaat cgcattttga caatatgcgc agttttttca tgggcaatgg
     2221 tttccagcaa atcaccgacc agcctgattt caagaacccg gtgttcgttg ccagctgggg
     2281 cgcctcggat gaagacttac tcaataaaac gcatgaccaa ctaatggcgc accatgccag
     2341 tggcaagcca ttttttaccc tggcattcag ctcgtctaac catgccccgt ttgaattccc
     2401 ggatgggcgg atcacactat atgaacagcc caaggcgact gataacaatg cagtgaagta
     2461 tgccgattat gcgatcggtg agttcattaa aaaagccaag gccagcccgt actggaacga
     2521 caccgtattt ttgattgtcg cggaccacga tatccgtgtc cgtggagatt cactggtgcc
     2581 gattgagcat ttccatattc cggccctgat tttgggtggt gcggtacagc cgaaaacggt
     2641 agatgcgatt gccagccaga ttgacttgcc ggttaccgta ttgtcattga tgggcataga
     2701 agcccagcac ccgatgaccg ggcatgattt gtccaatctg ggtgcagatt atcgtggccg
     2761 cgccatgatg cagtacaact ataacttcgg ctggatgcag cagaagggaa cggatgctgc
     2821 tgaaaacgag gtcgtggtat tgcgagaagg taaaccgcct gcatttgggc actatgatcc
     2881 ggtcagcaag caattgcaaa atacgccagc ccctgcagat gccaatgatt tggcggagcg
     2941 tgccttggcc aacagtctgt taccggcttt gttgtataaa gaacagcgtt atcgtttgcc
     3001 tgagtaggga tcgttttgaa gaagcctcaa ccttccatgc tcacgcgcca ggtcatcatt
     3061 gatggcctga aatatatttt ctccgggcgc ttgtggctaa aaaatagtca cctgcccgcg
     3121 attaatcaca cggttccaga ggatttcacc gggttgtgtg tcgccagcaa cgcggacccg
     3181 gccacagaca gttatgtgtt ggagcagttg ctggcactag gtgtcaagcg tgtacgcctg
     3241 gatatcagca atgacgaagc gcggcctttc caggaacgtt ttttacgcca gctgctggct
     3301 aacggtttct ctgtgaccgt acatgcggta cagccttacg aggacgccaa gaatatgcgt
     3361 agcagccagg cgcaggcagg ctggaagcaa tttatagaag aactttgcca ggcctacgca
     3421 ggtagcatcg ctgcgcttga aattggcaac acagtgaatc gtaaaaagtg ggctggctat
     3481 gatatgcctg ggtttatggc cgcctggcag attgcttatg acctcgcccg gcagtaccag
     3541 gtgacggtga tcggtcccaa tattcaggat tttgagccgc tgtacaacat cag
//
LOCUS       DATMSK010000232         3897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_417987,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000232 DATMSK010000000
VERSION     DATMSK010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417987"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2123)
                     /locus_tag="VNZ84_07715"
     CDS             complement(<1..2123)
                     /locus_tag="VNZ84_07715"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWU66882.1"
                     /translation="MYPDAPVPVADSAASIVRVVLDVPLDRWFDYVNPGLPIREGNRV
                     VVPFAGRQLVGIVMALVTETDVPQSKLKSILQVFDDVPFDAATLKLLRFCADYYHYPF
                     GQTVLSSLPLRLRQIKPAVSRKMWLYRLVQSIPDDFVGKRQQVLSRLVALLQQHDECS
                     EAMLSEVSATWRKAMQLLLDGGYVSKREVIATRPSMPASGLAPTLNDEQAYAVNAILG
                     NLNRFQPWLLFGVTGSGKTEVYIRLLEKVLAEPQAQALIMVPEINLTPQLESRFRSRL
                     SQFPLATLHSHLSESERLQNWQAAQSGAARIVIGTRLSIFTPMPHLKLMILDEEHDTS
                     YKQQDGMRYHARDVALVRAKQCNIPVVLGSATPSLESWYNAVGDRGHGERVARKPSYR
                     LLTLHKRAAAQAKLPRIFCIDTTHSPPEAGLTPQLKQAIELRLQRGEQSLLFINRRGY
                     APVLHCSACQWLADCRRCSAKAVLHLRQRVLRCHHCGDEQPIPVQCPSCGNPDLRPVG
                     QATQRIEETLQQLFPQARVARVDRDTIQAKDALTELLSAVHAGQIDILIGTQMLAKGH
                     DFANLTLVGVLDTDSALYSPDYRASERLFAQLMQVAGRAGRGDKPGEVLIQTAFPQHA
                     LFEALREQNFAEFADNLLQERMAMQFPPATYIAVMKAEATEYSLVEQFLTDFSQAARQ
                     YVAEQAETLSDQPLIYDPVRPGIARL"
     gene            2183..3841
                     /gene="argS"
                     /locus_tag="VNZ84_07720"
     CDS             2183..3841
                     /gene="argS"
                     /locus_tag="VNZ84_07720"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWU66883.1"
                     /translation="MKQTITELILQAVKPLLEDTSSLNIILERPKSAEHGDFATNIAM
                     QLAKPLKQNPRVIAQSIIDSLPANDVISKVEIAGAGFINFFLSADSKQGIVKDIFTAG
                     EAFGRNTAGQQQKVQVEFVSANPTGPLHVGHGRGAAVGDCLCRLLDANGWDVTREFYY
                     NDAGAQIDNLTKSVQARAQGISPEDAAFPEDGYRGDYIAEIAQAYLAKATVTADDMQV
                     TASGDMNDAESIRKFAVAYLRHEQDLDLQAFQIKFDVFSLESALYSEGKVENTVQALI
                     ASGHTYEQDDALWLRTTDFADDKDRVMRKKEGGYTYFVPDVAYHREKWNRGFKRVINE
                     QGADHHSTITRVRAGLQALNVGIPQGWPEYVLHQMVTVMRGGEEVKLSKRAGSYVTLR
                     DLIEEVGCDATRYFLAARSPDSQLVFDIDLARAQTNDNPVYYIQYAHARICSVLNQWG
                     GDSASLADAELSPLQSAAETALMQSLSQYPEVVSTACQALAPHMIANYLKELASALHS
                     YYNDTKFLVDESQIKLARLALITATRTVLRNGLALLGVSAPEKM"
BASE COUNT          990 a         1053 c          988 g          866 t
ORIGIN      
        1 agcctggcaa ttccggggcg tacaggatca taaatcaggg gctgatcgct cagtgtttca
       61 gcttgctcag caacatactg ccgggcagcc tggctaaaat ctgttaagaa ctgctcaacc
      121 aaactgtact cggtcgcttc tgctttcatg acggcaatgt aagttgccgg tggaaattgc
      181 atggccatac gctcttgtaa caaattatca gcaaactcag caaagttttg ttcgcgtaac
      241 gcctcgaata gggcatgctg cggaaaagcg gtttgtatca acacctcccc aggcttgtcg
      301 ccacgacctg cacggcccgc gacttgcatc aattgggcga acaggcgctc actggcacga
      361 taatccgggc tgtataacgc actgtcagta tccagcacac ctaccaacgt caagttggca
      421 aagtcatggc ctttggccaa catttgtgtg ccgatcagaa tatcaatttg accagcatgc
      481 accgcggata acagctccgt taaagcatct ttggcctgta tggtgtccct atccacgcgc
      541 gccacccgcg cttgcgggaa taattgctgc aaggtttcct caatacgctg cgtcgcttgg
      601 ccaaccgggc gtaaatcggg attgccgcaa ctaggacatt gcaccggaat cggctgctca
      661 tccccacagt ggtgacaccg caacacgcgt tgccgtaaat gcaagacggc tttggcagaa
      721 caacgtctgc aatcggccag ccattgacag gctgaacaat gcaacaccgg cgcataaccg
      781 cgtcggttaa taaacagcag gctttgctct cctcgttgta aacgaagctc tatagcttgt
      841 tttaattgag gggtcaaacc ggcttctggt ggactatgcg tggtatcaat acaaaaaatg
      901 cggggtaatt tagcctgagc cgcggcacgt ttgtgtagcg tcagcaaccg ataactgggt
      961 tttctggcca cacgctcacc atgccctcgg tcaccaacgg cgttatacca actttcgagc
     1021 gaaggcgtgg cactgccgag caccacagga atattgcatt gtttggcgcg caccagcgcc
     1081 acatcgcgcg catgataccg catgccatcc tgctgcttgt aagaagtatc atgctcttcg
     1141 tccaaaatca tcagcttgag gtggggcatg ggcgtaaaaa tactcaagcg cgtaccaatc
     1201 acgatccgag ccgcgccaga ttgggcggct tgccagtttt gcaagcgctc actttcactc
     1261 aaatggctat gcagcgtggc caagggaaac tgacttaaac gactacgaaa ccggctttcc
     1321 agttgtgggg tgaggttgat ctcgggcacc ataatcaacg cttgagcctg gggctcggca
     1381 agcacctttt ccaacaagcg gatatatacc tcggttttgc ccgagcccgt gacaccaaac
     1441 aacagccagg gctggaaacg attcaagttg cctaaaatcg cattgaccgc atacgcctgt
     1501 tcatcgttaa gcgtcggtgc caggcctgag gccggcattg aagggcgggt ggcaatcact
     1561 tcacgcttac tgacatagcc gccatcaagt aacagctgca tcgctttacg ccaggttgca
     1621 gacacttcgc tcagcatagc ctcactgcat tcgtcatgct gctgcagcaa tgctactaat
     1681 ctggacaaca cttgttgccg cttacccaca aaatcatccg ggatagattg caccaagcgg
     1741 tatagccaca ttttgcggga gaccgcaggc ttaatctggc gtaaacgtaa aggcaaacta
     1801 gacagcacgg tttgaccaaa aggatagtgg taatagtccg cgcaaaatcg cagcagcttt
     1861 aaagtcgctg catcaaatgg cacatcgtca aacacttgca atatggattt gagcttggat
     1921 tgtggcacgt cggtctcggt caccaacgcc atcactatgc caaccagttg ccgcccggcg
     1981 aaaggcacca ccacacgatt accctcccgg attggcaagc caggattcac ataatcaaac
     2041 cagcggtcca gtggcacgtc taataccaca cgcacaatac tggcggcgga atcagcaaca
     2101 gggacgggag catcaggata catgggtgaa aatggtttat taggttaaaa tggcggtttg
     2161 actaatcagt accattttaa ccttgaaaca aacgatcaca gaactcattc tgcaagcggt
     2221 caaaccgctg ctagaagaca cttccagcct gaatattatt cttgagcggc caaagtctgc
     2281 cgagcatggg gattttgcga ccaatatcgc catgcagttg gccaaaccgt taaaacaaaa
     2341 tcctcgtgta attgcacaaa gcattatcga cagcctgcca gcaaatgatg tcatcagcaa
     2401 ggttgaaatc gctggggctg gctttatcaa tttctttcta tcggctgaca gcaagcaggg
     2461 gattgttaaa gatattttta cggcgggtga ggcctttggc cgcaacactg ccgggcagca
     2521 gcaaaaagtg caggtcgaat ttgtttccgc taacccgacc ggtcccttac acgtcggcca
     2581 cggacgtggt gccgctgtcg gtgattgctt gtgccgcctg ctggatgcca atggctggga
     2641 cgtcacacgt gagttttact acaacgatgc cggtgcccag attgataacc tgaccaagtc
     2701 cgtgcaggcc cgtgcgcaag gcatttcccc tgaagacgcg gctttccctg aagatggtta
     2761 tcgcggtgac tatattgcag aaattgccca ggcttacctg gccaaagcca cggtcactgc
     2821 agatgacatg caagtgacag ccagtggcga tatgaatgac gccgagtcaa tccgcaagtt
     2881 tgccgtggct tatttacgtc acgagcaaga cctggattta caagccttcc agatcaaatt
     2941 tgatgtgttt tcgctggaaa gcgctctcta cagcgaaggc aaagtagaaa acaccgtgca
     3001 ggcactgatc gccagcggtc atacatatga gcaggatgat gcattgtggc tacgcaccac
     3061 ggactttgcg gatgacaaag atcgcgtcat gcgcaagaaa gagggcggtt acacttattt
     3121 tgtcccagac gtggcttacc accgcgaaaa atggaaccgc ggtttcaagc gtgtgattaa
     3181 cgagcagggc gcagaccatc acagcaccat tacgcgtgtg cgtgccggcc tgcaagcgct
     3241 caatgtaggc attccacaag gctggccaga gtatgtgttg caccagatgg tgaccgtcat
     3301 gcgcggaggt gaagaggtca aactctccaa acgcgcaggc agctacgtca ccctgcgtga
     3361 cctgattgaa gaggtcggtt gcgatgccac gcgctatttc ctggctgcgc gcagcccgga
     3421 ctctcaactg gtatttgata ttgacctggc acgcgcacaa accaacgata acccggttta
     3481 ctatatccag tatgcacatg cccgtatttg cagcgtgctc aaccaatggg gcggtgacag
     3541 cgcaagcctg gctgacgctg aattgtcccc cctgcaaagt gctgccgaaa ctgcactcat
     3601 gcaaagcttg agtcaatacc cggaagtcgt ctctacggcc tgccaggcac tggcaccgca
     3661 tatgattgcc aactacctca aggaactagc cagtgccttg catagttatt acaatgacac
     3721 caaatttttg gtagatgaat cgcaaatcaa actggcacgc ctggcactta ttacagcgac
     3781 gcgtactgtg cttcgcaacg gcctggcctt actgggcgtc agcgcaccag aaaaaatgta
     3841 agccttttta atgaccgcct gaactgattg gtaaattaat acatgagccg cgattac
//
LOCUS       DATMSK010000233         7095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_347391,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000233 DATMSK010000000
VERSION     DATMSK010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347391"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1021)
                     /gene="dapB"
                     /locus_tag="VNZ84_07725"
     CDS             complement(215..1021)
                     /gene="dapB"
                     /locus_tag="VNZ84_07725"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWU66884.1"
                     /translation="MLKVVIAGVSGRMGHALLDGVFSDNGLQLHAALDRAESAMIGRD
                     AGEQFGKVSGVKITSDIKAALAGADVLVDFTRPEASMQYLQACQQANVKLVIGTTGFS
                     EAEKASIESAAKTIGIVFAPNMSVGVTLLINLVEQAARVLNEGYDIEVVEMHHRHKVD
                     APSGTALRLGEAAAKGIDKALKDCAVYAREGVTGEREAGTIGFATLRGGDVVGDHTVV
                     LAGVGERVELTHKASSRATFAQGALRAAKFLADKPQGLFDMRDVLGFEKN"
     gene            complement(1074..3398)
                     /gene="bamE"
                     /locus_tag="VNZ84_07730"
     CDS             complement(1074..3398)
                     /gene="bamE"
                     /locus_tag="VNZ84_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HWU66885.1"
                     /translation="MRNSILFVLVVASLESGCGAKLPSVKPFKMEVQQGNVVTSKMLL
                     QLRPGMTRSQVRYIMGTPLIVDSFRDNRWDYFYELRKEGKIIEKRRVILDFDKDSLVS
                     VRGDVIPSAENPDVKTIVEAPQKKGEEAKAQSWTERLKFWESEEGAASDKATEATTAA
                     AATAAVTPESKVAEPAKAESAKVQPAAEANNTSNPSLTPPVAEEEVVPYIPEGEYQAA
                     PTPEEMAKGNLDATANQVTEAKTHPVNEKEMAAQIADTVEPPPVFQQEPMAEPVAAAE
                     PEPVLPHAAKATPAAKSVVAPVVAAASVAAATTAKPAESKPIEQKSTLQRTEVQKTVP
                     QAPGAEATIEDDEVIPYIPEGEYVAPVIPTEAEMVKGNMAEANAPSTDAEARQVTEKG
                     VAPKADAAAEPSPTFVAEQLPEPEPEPELPPPPPPSASKPAVKEVAPVAALAAAEDKP
                     VVAQPEAEQPAAKVDEPTPSASPVAAQEAPAGDAEINQAVAAWAQAWRSKDVKSYLAA
                     YAPDFVPEGLPSRKAWEAQRKQRLSAGQGTITLVLNDVQIQRDGTTATVQFEQTYAAK
                     AYKDKLAKTLEMRYEPVQKRWLIARERVAPITTLPAVAAAPSKVDVIEPVTPTTQATP
                     TVAPAADVSVESAIDVWAQAWRSKNINAYLSAYSPEFVPEGLPSKSAWEAQRKKRLSP
                     QQGKISLDITNVSVTRDGDTAVATFTQKYAAKAYRDEVVKRLQLKLDAASNRWLIVRE
                     GVASGAEVTASKQQVTAPEESVEHLDGVLEQIGF"
     gene            3508..3927
                     /gene="fur"
                     /locus_tag="VNZ84_07735"
     CDS             3508..3927
                     /gene="fur"
                     /locus_tag="VNZ84_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HWU66886.1"
                     /translation="MHDPKDLKNAGLKATLPRLKILELFENSEQRHLSAEDIYKIMIT
                     NGEDVGLATVYRVLTQFEQAGLLVRHHFESGKAVFELNEGSHHDHIVCIKCGRVEEFY
                     DEEIEKRQKTAAEKYGFSMQDHSLTIYGVCKKQPCTE"
     gene            3984..4499
                     /locus_tag="VNZ84_07740"
     CDS             3984..4499
                     /locus_tag="VNZ84_07740"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HWU66887.1"
                     /translation="MADLTPIPEHYATGFPPIVGLNPRILILGSLPGAESLKQQQYYA
                     HPQNAFWKMLEALFNIPVNASYTERCEGVKSAHLAIWDVCHSACRPGSLDSAISAETV
                     IANDIHGLLAENSTIKLIAFNGNTAATLFKKHIKALPLVDTIILPSTSPAHASLRFAE
                     KLERWQTLQTR"
     gene            complement(4492..6150)
                     /gene="recN"
                     /locus_tag="VNZ84_07745"
     CDS             complement(4492..6150)
                     /gene="recN"
                     /locus_tag="VNZ84_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWU66888.1"
                     /translation="MLQALSIRDFVIVDTLELEFSAGYTALTGETGAGKSILIDALSL
                     SLGARNEGDVTRKGCEKAEISTTFDIVDNQAARDWLLAQEMDVDDALVLRRVIYADGR
                     TRAFINGASATVGQLREIGETLIDIYSQNAHHSLLKVATQREILDAYAQVSPLAKQVT
                     KLYLDWSQLQQQQLAYEKNASQFADELAELRDSTRELKQLGFSSDEWLVLQQEHVRLS
                     NGASLLSGMEASLHMMSEGDEVNALDLLSQAQTKLAELQAIDAGLQPIAETLDSAVVQ
                     LEEASRALNRYLQKSELDPERLAEVEARIQAIHGAARKFRIKPDELPELLAQQLQRVT
                     ELEGFADDGALAKQVQLAWQAYHDQAGQLSQSRQQAAHKLAKTITEQMQALSLKGGQF
                     AVALTPGEPAAHGLEQVEFLVAGHAGVEPRPLNKVASGGELSRISLALQVTTASLGTV
                     PCMIFDEVDVGIGGGVAEVVGRLLSELGRHRQVLVITHLAQVASQAQQHLQVSKSEQQ
                     GATLSRIRPLGADERVEEIARMLGGLQITETTRNHAREMLGLIT"
     gene            complement(6170..7039)
                     /locus_tag="VNZ84_07750"
     CDS             complement(6170..7039)
                     /locus_tag="VNZ84_07750"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HWU66889.1"
                     /translation="MQSTFRSVAIVGKYMDAAALQQMQSDLSRLAQHLLLHGLHVCIE
                     ERTAQFIEDKGFELASLDDIGSKSDLVIVMGGDGTMLSVGRALRDTGVPLIGINRGRL
                     GFLTDLQTDHMLDDLDRILQGEHQLESRILLQSVVTRGEQVIKQTVALNDVVIKSAFR
                     LIELEVHVDGQFVSRQRSDGLILTTPTGTTAYGLSAGGPIMHPDLEAISIVPISPHTL
                     SYRPITVPACSVIEVIVVNAADAQISYDGQGQYPLAVGDHVRIERAPQQIKLVHPKDY
                     CYFDMLRNKLNWG"
BASE COUNT         1721 a         1992 c         1628 g         1754 t
ORIGIN      
        1 tttgatacga aacacatctt ttagagggtt cacaatggcc agaaacgaaa ttcaagctac
       61 agtgaataga tgtgcaaaac gggaaaagct tgttggactc ttcccgtttc agaattgcgg
      121 cattatagcc taaattggca acccactcaa gggcaactaa gaggcaaaaa aacgattgat
      181 gcttaaacct gatcgatgaa aaacctactt gatatcagtt tttctcaaac cccagtacat
      241 cacgcatatc aaacaaccct tgcggcttat ctgccaggaa cttggctgcg cgcaatgcac
      301 cctgtgcaaa cgtggcacgg cttgaggctt tatgtgtcaa ctcgaccctt tcacctacac
      361 ccgccagcac cacggtatgg tcaccaacca catcaccacc acgcaacgta gcaaaaccaa
      421 ttgtgcctgc ttcacgctca ccggtaacgc cttcgcgggc atatacggca caatccttga
      481 gtgctttatc aatgcctttg gctgcagcct cgcccaaacg tagtgccgtg cctgacggcg
      541 cgtccacttt gtggcgatga tgcatttcaa ccacttcaat atcgtagcct tcattgagca
      601 cgcgtgcggc ctgttcaaca aggttaatca agagcgtcac acccacactc atattgggtg
      661 cgaatacgat gccaattgtt ttagctgcgg attcaatact tgctttttca gcctcactaa
      721 accctgtcgt accgatgacc agcttcacat tcgcctgttg gcaggcttgc aaatactgca
      781 tactggcttc cggacgcgtg aaatccacca gcacatcagc accagctaac gcggctttga
      841 tatctgaagt aatttttact ccgctcacct tgccaaactg ctcgcctgca tccctaccta
      901 tcatggcgct ttcagcacgg tcaagtgccg catgcaactg caagccgtta tcagaaaaaa
      961 cgccatccaa taaggcatgc cccatacgac cagacacacc agcaatcact actttcaaca
     1021 taactcgctc aataactctt taataattta aaccacatcc atcagctatt gtattaaaaa
     1081 ccgatttgtt ccaacacacc gtccagatgc tctacactct cttcaggcgc agtcacctgc
     1141 tgtttgctgg cagtcacctc tgcgccactc gcgacacctt ccctgacaat cagccaacgg
     1201 ttacttgcgg catccagctt caattgcagg cgttttacca cttcatcacg ataggcctta
     1261 gcagcatatt tttgcgtaaa tgtggctacc gcagtgtcgc cgtcgcgggt cacgctcaca
     1321 tttgtgatat ccagactgat cttgccctgc tgtggtgaca ggcgcttttt acgctgcgct
     1381 tcccaggcac tcttgcttgg caagccttcc ggcacaaact ctggagagta agccgacagg
     1441 taggcattga tgtttttact gcgccaagcc tgcgcccaaa catcaatcgc cgattcaaca
     1501 ctgacatccg ccgcaggcgc caccgttggt gtggcttgtg tcgttggcgt gaccggctcg
     1561 atcacatcca ccttggatgg tgctgcagca accgctggca aggtggtgat aggtgcaaca
     1621 cgttcacgtg caatcaacca gcgtttctgc actggttcat accgcatttc cagtgttttg
     1681 gccaatttat ccttataggc tttcgctgca tatgtttgtt caaactgcac ggtcgccgtt
     1741 gtaccgtcac gctgaatttg cacgtcattc aagactaatg taatggttcc ctgacctgca
     1801 gacagccgct gcttgcgttg cgcttcccac gcttttctgg agggcagccc ttccggcacg
     1861 aagtcaggtg cataagcagc caggtaactt ttaacatcct tgctacgcca ggcttgtgcc
     1921 catgcggcca cggcctggtt gatttcagca tcccccgcag gtgcttcttg agccgcaaca
     1981 ggcgaggcag atggtgttgg ctcatccacc ttggccgctg gctgctcggc ttcaggttgg
     2041 gcaaccactg gcttgtcttc ggccgcagcc agtgctgcaa ctggtgcaac ctctttcacg
     2101 gcaggcttgc tggcagatgg cggaggtggt ggtggcaatt caggttccgg ctcaggttct
     2161 ggcaactgct cagccacaaa ggtcggtgat ggttctgcgg ccgcatccgc tttgggcgcc
     2221 acgccttttt cagtcacttg acgtgcttca gcgtcagttg aaggcgcgtt tgcctctgcc
     2281 atattcccct taaccatttc cgcttcagta ggaatgacag gagcgacata ttcaccttca
     2341 ggaatgtatg gaatcacctc atcatcttca atggtcgctt ccgcaccagg cgcctgaggc
     2401 actgtttttt gtacctcagt gcgttgtaat gtggattttt gctcgatagg cttggactcg
     2461 gcaggcttgg ctgttgttgc tgcagccacg gaagccgcag caacaaccgg cgcgacaacg
     2521 ctcttcgcag caggagtagc ttttgctgca tgtggcaata ctggctcagg ctctgccgcc
     2581 gcgacaggct ccgccatggg ttcctgctgg aaaacagggg gtggctcaac cgtatcggca
     2641 atttgagcgg ccatttcttt ctcattgacc ggatgggttt tcgcttcagt cacctggttt
     2701 gctgtggcat ccaggttacc tttagccatt tcttctgggg ttggagctgc ctggtattca
     2761 ccttcgggaa tataaggaac aacctcttcc tctgccacag gcggcgtcaa agacggatta
     2821 gaggtattat tcgcctcggc agcaggctgc actttcgcag attctgcttt ggcaggttca
     2881 gcaactttgc tctctggagt gaccgcagca gtggcagcag cggctgtggt cgcttctgtt
     2941 gctttatcac tggccgcacc ttcctcagac tcccagaact tcagcctttc cgtccaactc
     3001 tgtgcttttg cttcttcgcc ctttttctga ggcgcctcta caattgtttt tacatcagga
     3061 ttttctgcag atgggatcac atcaccgcgc accgatacca ggctatcctt atcaaaatca
     3121 agaatcaccc tacgcttctc gatgatcttt ccctctttgc gcaactcata aaagtaatcc
     3181 cagcggttat ctcggaaact atccacaatt aatggtgtcc ccatgatgta gcgcacttgc
     3241 gagcgcgtca tccctgggcg caactgcaac aacatcttgg aagtgacaac attaccttgc
     3301 tgaacttcca tcttgaaagg cttgactgaa ggaagcttgg caccgcaccc actctccaga
     3361 gatgcgacaa ccaatacaaa taagatagaa ttacgcattg gattcacttt ataatcggta
     3421 tattcataac agtagtaata taccacgtag tttcgccaat caaatacgcc ggacgactta
     3481 accacttaca aaacatggac aaaacctatg catgatccca aagacctcaa aaatgcaggc
     3541 ctgaaagcga cgctacctcg gctgaaaatc ctggagttgt tcgaaaacag tgagcaacgc
     3601 catctctcag ctgaagatat ttacaagatc atgattacca atggtgaaga tgttggcctg
     3661 gcaaccgttt accgcgtact gacccagttc gaacaagcag gactattggt tcgccaccat
     3721 tttgaaagtg gcaaggccgt gtttgaactg aatgagggta gtcaccacga ccacatcgta
     3781 tgcatcaaat gcggacgcgt cgaagagttt tacgatgaag aaattgaaaa aaggcaaaaa
     3841 accgcagctg aaaaatatgg attcagcatg caggatcact cactcaccat ttatggtgtg
     3901 tgtaagaaac agccctgtac tgaataattg cccgggttaa tggtgaggtg tgacattaac
     3961 ccgttagtac tccttgatac gatttggctg acctaacacc cattcctgaa cactacgcca
     4021 ccggttttcc gcccattgtg ggcctcaatc cacgtatatt gattctgggc agtctaccgg
     4081 gcgccgaatc tcttaaacag caacagtatt atgcgcatcc gcaaaatgcg ttttggaaga
     4141 tgctggaagc gttattcaat atcccggtaa acgcctctta caccgagcgt tgtgaaggtg
     4201 tgaagtcagc tcacctggcg atttgggacg tttgccattc agcctgccgc ccgggcagct
     4261 tagatagcgc catcagcgca gaaacggtga tcgccaatga tattcacggc ttgcttgcag
     4321 aaaactctac aatcaaacta attgcgttta acgggaatac tgccgccacc ctatttaaga
     4381 aacatattaa agcattgccg ctagtagaca ccattatctt accctccacc agtccggccc
     4441 atgccagcct gcgctttgca gaaaagctgg agcgttggca aacattacaa actaggtgat
     4501 gagtcccaac atttcccggg catgattgcg agtggtttca gtgatttgca agccacccag
     4561 catacgggca atttcttcta cgcgctcatc cgcgcctaag ggacgaatgc ggcttaatgt
     4621 ggcgccttgc tgctcagact tgctgacctg taaatgctgc tgcgcctgcg aggcaacttg
     4681 tgccaggtgc gtaatgacca acacctgacg gtgccggccc agttcgctaa gcaaacgccc
     4741 caccacttct gccacgccac caccaatacc gacatcgacc tcatcaaaaa tcatgcatgg
     4801 cactgtgccc aaggaggctg ttgtcacctg tagtgccaag ctaatgcgag acaactcacc
     4861 gccggaggcg actttattga gcggacgagg ttcaacgccg gcatgaccag ccaccaggaa
     4921 ctccacttgc tccaaaccat gcgccgcagg ttcaccaggc gttaaagcaa ccgcaaactg
     4981 gccacctttt aaggacagcg cctgcatttg ttctgtgatg gttttagcca gcttatgtgc
     5041 agcttgttgc ctagattgag aaagttggcc agcttgatca tgataagctt gccaagccaa
     5101 ttgtacctgc ttagccaagg caccatcatc ggcaaaaccc tccaactctg tgacacgttg
     5161 cagttgctgg gccagcagtt caggcagctc atcaggttta atgcggaact tgcgcgcagc
     5221 gccatgtatt gcctggatac gcgcttctac ctcagcgagg cgttcgggat ccaattcact
     5281 tttttgcaga tatcgattga gtgctcgact ggcttcctcc agttgcacca ccgcagaatc
     5341 cagagtttca gcaatcggct gcaatccggc atctatcgcc tgtaactcgg caagtttggt
     5401 ttgtgcttgc gagagcaagt ctagcgcatt cacctcatcg ccttcactca tcatgtgcag
     5461 gctggcttcc atgccactca acaaactggc accattgctt aagcgaacat gctcctgctg
     5521 taataccagc cattcatcgc tggaaaaacc cagttgcttc aactctcgtg tactgtctcg
     5581 cagctcagcc agctcatctg caaactggct ggcatttttc tcataggcca gttgctgctg
     5641 ctggagttgc gaccagtcta ggtataactt agtcacctgt tttgccagtg gcgacacctg
     5701 tgcataagca tccaggatct cgcgctgggt agccaccttt aacaaagagt gatgagcatt
     5761 ctggctgtaa atatcaatga gggtttcgcc tatttcgcgc aactggccta ccgtggctga
     5821 cgcaccatta ataaaagcac gggtacggcc atcggcataa atcacgcgcc gtaataccaa
     5881 agcatcatct acatccattt cctgcgctag cagccagtcc cttgcggcct gattatcgac
     5941 tatatcaaaa gtggttgaaa tttctgcctt ttcacacccc ttgcgcgtga cgtcaccttc
     6001 attacgtgca cccaggctta aagatagggc atctatcagg atggacttac ccgcaccagt
     6061 ctctccagtg agcgcggtgt atcctgctga gaactcaagt tccagcgtat caacaatgac
     6121 aaagtctcgg atagaaaggg cttgtagcat ggtgaatggc agggctgtat cagccccagt
     6181 ttaatttgtt gcgcaacata tcaaaataac aataatcttt ggggtgcacc agcttgattt
     6241 gctgtggcgc acgctcaatc cgcacatgat cacctacggc cagtggatat tggccctggc
     6301 catcataact gatctgggca tccgcagcat tcacaacaat cacctcaatc acgctacagg
     6361 caggcacggt aattggccgg taactaagcg tatgtggact aatcggcaca atcgaaatcg
     6421 cttcgaggtc aggatgcata atcgggccgc cggcagacaa accataagca gtcgtcccag
     6481 tcggcgtggt taaaatcagg ccatcagaac gctgacgaga cacaaactgc ccgtcgacat
     6541 gaacttccag ctcaatcagg cggaaagcac ttttaataac cacatcattg agcgcaactg
     6601 tttgtttaat cacttgctcg ccgcgggtca ccacagattg caacaatatg cgtgactcca
     6661 gctgatgttc tccctgcaaa attctgtcca gatcatccag catatgatcg gtttgcaaat
     6721 ctgtcagaaa tccaaggcgg ccgcggttaa tgccaatcag cggcacgcct gtatcccgca
     6781 gagcacggcc aacactcagc atggttccgt ctccgcccat cacaatcacc aggtctgact
     6841 tgctaccaat atcatctagg ctcgcgagct caaaaccttt gtcttctata aactgcgcag
     6901 tacgctcttc aatacacaca tgcaaaccgt gcaacaacag gtgctgtgcc aaacggctta
     6961 aatcactttg catttgctgc aaagcggcgg cgtccatgta ttttccaaca atggcgactg
     7021 aacggaatgt agattgcatg gccgcattaa agcatagaag cctcgaactg aaaagcacaa
     7081 ggggctgtta tcgca
//
LOCUS       DATMSK010000234        13228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_229344,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000234 DATMSK010000000
VERSION     DATMSK010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13228
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229344"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VNZ84_07755"
     CDS             complement(<1..543)
                     /locus_tag="VNZ84_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWU66890.1"
                     /translation="MAQLNNGVAASVTQKQPLWLLAEVTYRCPLHCAFCYNPTDYDKH
                     TKNELSTEQWINALRDARKMGAIQLGISGGEPLLRDDIEEIVAEAHKLGYYSNLITSG
                     VGLTEKRIQAFKEGGLDHIQLSMHDVTEDISNFITNTRTFELKKKVAAMIKSHGYPMV
                     LNVVIHRYNIDQVGKILEMAE"
     gene            complement(561..845)
                     /gene="pqqD"
                     /locus_tag="VNZ84_07760"
     CDS             complement(561..845)
                     /gene="pqqD"
                     /locus_tag="VNZ84_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HWU66891.1"
                     /translation="MENNIQHSDIYAIALHHRFQWEEAQQSYVLLFPEGMIKLHGGAG
                     EILKRVDGKVSVGDIVADLKTTFPDAEDIENDILGMFDLAVGKAWLRKVN"
     gene            complement(881..1645)
                     /gene="pqqC"
                     /locus_tag="VNZ84_07765"
     CDS             complement(881..1645)
                     /gene="pqqC"
                     /locus_tag="VNZ84_07765"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HWU66892.1"
                     /translation="MNAVTQAHLPWTREEFEAKLREKGQGYHIYHPFHVMMYEGKLTK
                     EQLQCWVANRFYYQIAIPMKDAAILSNCPDKEVRKQWIVRITDHDGVDGQIGGIEAWI
                     KLGEAVGLTREQVTSLELVSPGVRFAVDAYINFAKQRPWQESVCSSLTELFAPHIHQQ
                     RISSWPSMYPWVKEEGLMYFKKRLTEARRDVEQGLSVTLDYFSTSREMQVKALEILQF
                     KLNVLWVIADSIMLASTEIKTEGRDYLRQPVIKIEN"
     gene            complement(1655..2596)
                     /gene="pqqB"
                     /locus_tag="VNZ84_07770"
     CDS             complement(1655..2596)
                     /gene="pqqB"
                     /locus_tag="VNZ84_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HWU66893.1"
                     /translation="MHIHVLGAGAGGGFPQWNCNCQNCDGLRKGTIKAKRRTQSSICV
                     SSNGVDWVLFNASPDVLQQIQEFAPLQPGRGVRDTGIQGIVLIDAQIDHTTGLFMLRE
                     GQVKREIYCTKMVYEDLTTGNQILNILGHYCGINHHEIAIDASDVSNAHSFEIPGVPN
                     LRFTAVALKSAAPPYSPHRNDPHPGDTIGVLIEEISTGKKCWYSPGLAEIEPHLPPLM
                     NQADCIMVDGTFWTNTEMLDMGLMTKTARSIGHNPQSGPGGMIEELDKFGMNKAVRKV
                     LIHINNTNPILREDSAERAILDEHKIEVAYDGMDIIL"
     gene            complement(2881..3963)
                     /locus_tag="VNZ84_07775"
     CDS             complement(2881..3963)
                     /locus_tag="VNZ84_07775"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWU66894.1"
                     /translation="MTVYEKLATDDVRVLEIKPLVKPAELLSRLQESTVSTQNILKTR
                     TAIHHILHQGDDRLLVIIGPCSIHDTDAGMEYAKRLLDVRNRLAGELLIVMRVYFEKP
                     RTTVGWKGLINDPHLDGTYDINSGLEKARRFLLDVNEIGVPAATEFLDVVSPQYTADL
                     VSWGAIGARTTESQIHRELASGLSCPVGFKNGTDGGVKVAIDAIKTAASPHHFLSMTK
                     EGESAIFATKGNEDCHVILRGGKAPNYNAQSVAAVCDQLADAGLAPVLMVDCSHGNSQ
                     KQYKNQIAVVDDVAVQVSGGDARIIGVMLESHLNEGRQDHSPGCSLNYGQSITDACLG
                     WEDSVAVLETLATAVKTRREKRAAEE"
     gene            complement(3960..5072)
                     /gene="hisC"
                     /locus_tag="VNZ84_07780"
     CDS             complement(3960..5072)
                     /gene="hisC"
                     /locus_tag="VNZ84_07780"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWU66895.1"
                     /translation="MSSSVSLSELAPANIRAIAPYQGGKPISELAREMGLNEADIVKL
                     ASNENPLGMSPKAQMAVEEAIYEIARYPDGNSFVLRDAVSQKYGVQPAHIVFGNGSND
                     ILELAARAFLTAGDEAIYSQHAFAVYPLVTQAVGATGVVVPAVDFGHDLPAFLKAITT
                     KTKLIFVANPNNPTGTLISKPALKDFLRQVPKHILVVLDEAYDEYLSAADKSEAIGWL
                     AEFENLIISRTFSKAYGLAGLRVGFGLMHATLADLMNRVRQPFNVNSLAQVAAAVSLQ
                     DEDFVARSYAANQAGMAQVTQGLNKLGLSYIPSFGNFVSFKVTNASAVNQALLKHGVI
                     VRPVANYDMPDYLRVSIGLFSENARFLEVLEKILTT"
     gene            complement(5081..6163)
                     /gene="pheA"
                     /locus_tag="VNZ84_07785"
     CDS             complement(5081..6163)
                     /gene="pheA"
                     /locus_tag="VNZ84_07785"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWU66896.1"
                     /translation="MSDSLKQFRDKIDAIDAQVLALVNERAKLAREIGHLKDDGVIYR
                     PEREAQIIRRLQAENDGPLSPEAVSHIFRAVMSNCRALEKELAIAFLGPLGTYSEEAA
                     LKQFGEGRQAVVCGSIDEVFRTVEAGQADYGVVPVENSTEGAVGITLDLLLGSALQVV
                     GEVTLPVHHCLLSAQQDLQQITHVFSHAQSLSQCHEWLNKILPSAEREAVTSNARAAQ
                     MIHELVATQGTFAAAIASKRAAELFDLNILAENIEDDPKNTTRFLVLGNHTVAPSGQD
                     KTSLVMSAHNKPGAVLQLLEPLSRHGVSMTKLESRPSRQNLWNYVFFVDIEGHQQQPS
                     VQAALKELAERATFLKVLGSYPTAII"
     gene            complement(6187..7266)
                     /gene="serC"
                     /locus_tag="VNZ84_07790"
     CDS             complement(6187..7266)
                     /gene="serC"
                     /locus_tag="VNZ84_07790"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HWU66897.1"
                     /translation="MKQIFNFSAGPAVLPKPVLERAQAEMLDWHGSGMSVMEMSHRGK
                     EFTSILEKTEADLRQLLSIPANYKVLFLQGGAIAENAIIPMNLLNGKSADYVVTGSWS
                     KRSVEDAKAYGQINIAANAEATGFTTVPTFADWKLNKEAAYVHYCTNETINGVEFLDV
                     PETHGVPIVADMSSHILSRPIDVSKYGVIYGGAQKNIGPAGLCLVIVREDLLEQASPL
                     TPAVFHWKTQADNQSMINTPPTYSIYIAGLVFEWLLAQGGVDAIEKVNIAKADLLYHY
                     LDQTEFYDNLIQKEYRSRMNIPFRLRDESLNDAFLKAADTQGLLQLKGHRSVGGMRAS
                     IYNAMPIEGVQALVEFMQAFEQKNS"
     gene            complement(7275..9845)
                     /gene="gyrA"
                     /locus_tag="VNZ84_07795"
     CDS             complement(7275..9845)
                     /gene="gyrA"
                     /locus_tag="VNZ84_07795"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWU66898.1"
                     /translation="MSQSPDQFAKETLPISLEDEMRRSYLDYAMSVIVGRALPDVRDG
                     LKPVHRRVLFAMHELSNDYNKPYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQDFSL
                     RYMLVDGQGNFGSVDGDNAAAMRYTEIRMSKIAHELLADIEKETVDFGPNYDGSEQEP
                     LIMPTRIPNLLINGSSGIAVGMATNIPPHNLNEVLNACFALLKNPEISVDELIEFIPA
                     PDFPTAGIIYGTAGVKEGYRTGRGRVVMRGRTHFEDLEKGGRQSIIIDELPYQVNKKS
                     LIEKIAELVNEKKIEGISDLRDESDKSGMRVVIELKRGEVPEVILNNLYKQTQLQDTF
                     GMNMVALMDGQPRLLNLKQMLEAFLRHRREVVTRRTVFELRKARDRGHVLEGLAVALA
                     NVDEMIAIIKAAPTPPEAKKELMARSWHSPVVEEMLKRAAMEASRPEGLSVDFGLTPK
                     GYKLSDVQAQEILQMRLQRLTGLEQDKIVNEYKEVMDIIADLLDILAKPERVTAIIVE
                     ELNEIQKQFGDKRRSEIEQNTQDLSLEDLITPQDVVVTLSHTGYVKSQPLDEYRAQKR
                     GGRGKQAAATKEDDFIDKMFVANTHDYILCFSSRGRVYWLKVYEVPQGSRVSRGKPIV
                     NLFPLEEGEKINAILPVKEFDENHYVFMATAKGTVKKTALTEFSNPRKSGIIAINLDD
                     GDYLIGAEVTNGQNDIVLVSNGGKAVWFTEDDVRPMGRATRGVRGMKLAAKQQVLSLL
                     IAENDQQSVLVATENGYGKRTVLSEFRHSGRGTQGVKAIAISERNGLAIAAKLVTAED
                     EIMLITTGGVLIRTRVKEIRDMGRAAQGVTLINLGEGEKLSGLEKIVETDDDGDDSEE
                     "
     gene            complement(10054..11241)
                     /locus_tag="VNZ84_07800"
                     /pseudo
     CDS             complement(10054..11241)
                     /locus_tag="VNZ84_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830212.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
     gene            complement(11435..12970)
                     /gene="purF"
                     /locus_tag="VNZ84_07805"
     CDS             complement(11435..12970)
                     /gene="purF"
                     /locus_tag="VNZ84_07805"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWU66899.1"
                     /translation="MCGIIGIVGKNPVNQLLYDGLLVLQHRGQDAAGIVTCDGNTFHM
                     HKNNGLVKDVFHTRHMRNLIGNVGIAHVRYPTAGSSSAAEAQPFYVNSPFGIVLGHNG
                     NLTNSEQLKSEMFRQDLRHINTSSDSEVLLNVLAHEIEATSKSALLNSDMVFDAVAGV
                     HRRCKGAYAVVAMIANFGLLAFRDPHGIRPLVIGKQETDKGTEYIVASESVALDVLGF
                     TMLRDVEPGEAVFIDMDGNLFSRQCAENPKLTSCIFEYVYLARPDSVIDGVSVYQTRL
                     DMGTLLAEKIKREWADKQIDVVIPIPDTSRPSALQLGIALGLDYREGFIKNRYIGRTF
                     IMPGQALRKKSVRQKLNPIGIEFKGKNVLLVDDSIVRGTTSQQIVQMARDAGANKVYF
                     ASAAPPVRYPNVYGIDMPSRHELLATNRTDEEICAEIGADALIYQDLDALRESITKSN
                     PHMTEFDCSCFDGKYVTGDIDAAYLNRIESARSDMNKKQLPPNSTTQLDLNLLSTDLV
                     GVE"
     gene            complement(13002..>13228)
                     /locus_tag="VNZ84_07810"
     CDS             complement(13002..>13228)
                     /locus_tag="VNZ84_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HWU66900.1"
                     /translation="RVLGGIAGAGKGVLIVCILAMLAAMTDLPKDTRWTNAMLSSPVE
                     ALVLKLLPLMPSSIAEHVHLDHQRVDESAI"
BASE COUNT         3290 a         3572 c         2996 g         3370 t
ORIGIN      
        1 ttcagccatt tcaaggattt tgccaacctg gtcaatgttg tagcgatgga taaccacatt
       61 cagcaccatg ggatagccat gggacttgat catggcagcc acttttttct tcaactcaaa
      121 cgtgcgggta ttggtaataa agttgctgat atcttcagtc acatcatgca tggaaagctg
      181 gatgtgatcc aggccgcctt ctttaaatgc ctggatgcgc ttttcagtca ggcccacacc
      241 ggaggtgatc aggttgctgt aatagcccaa cttatgcgct tcagcgacaa tctcttcaat
      301 atcatcacgc aataaaggct caccaccaga aatgcccaat tgaatagcgc ccattttacg
      361 ggcgtcacgt aaagcgttga tccactgctc tgtgcttaat tcatttttgg tgtgcttgtc
      421 ataatcagta gggttataac aaaatgcaca atgcagcggg caacgatagg tcacctcggc
      481 caatagccat aaaggctgct tttgagtcac acttgctgca acaccattat tcaactgtgc
      541 catcatgccc tcctttcgga ttaattcact ttgcgtaacc aggctttacc aacagccagg
      601 tcaaacatgc cgaggatgtc attctcaata tcctcggcat ccggaaaggt tgttttcaag
      661 tcggccacaa tatcgcctac actcactttg ccgtcgacac gcttgagtat ctctcccgcg
      721 ccaccatgca gcttgatcat gccttccggg aacaagagga cataactttg ctgtgcttct
      781 tcccactgaa aacggtggtg caaggcaatg gcataaatgt ctgagtgctg aatattgttt
      841 tccatatctc tactttcaaa tgcccaagtc aggcgacaag ctaattttca atcttgatga
      901 ccggctgacg caggtaatcg cggccttcgg ttttaatctc ggtggaagcc agcataatag
      961 agtctgcgat cacccacagc acattcagct tgaattgcag gatttccagc gctttgacct
     1021 gcatttcgcg cgaagtactg aaataatcca aagtcacact caagccctgc tcaacatcgc
     1081 gccgtgcctc agtcaggcgt tttttgaaat acatcaagcc ctcttcctta acccaaggat
     1141 acatactggg ccaggagctg atgcgttgct ggtggatatg tggtgcaaac aactcggtca
     1201 ggctggagca cacgctttcc tgccagggac gctgtttagc aaagttgata tacgcatcca
     1261 ctgcaaaacg aaccccgggg gaaaccagtt ccaggctagt cacttgctca cgtgtcagcc
     1321 caacagcctc acccaattta atccaggctt ctatgccgcc aatctggcca tccacgccat
     1381 cgtggtctgt aatacgcacg atccattgct tgcggacttc tttgtctggg caattagaca
     1441 agatggcggc atccttcatc ggaatcgcga tttggtagta aaaacggttg gcgacccagc
     1501 attgcagctg ctctttggtt aacttgcctt catacatcat cacgtgaaat ggatgataaa
     1561 tatggtaccc ctgccctttt tcacgaagtt ttgcctcaaa ctcttcacgg gtccaaggca
     1621 agtgtgcctg agtcactgca ttcattttgc tctcctatag aatgatgtcc atgccgtcat
     1681 acgccacctc gattttgtgt tcatcgagga tcgcacgctc ggcggaatct tcccttaaaa
     1741 tcgggttggt attgttgata tgaatcagca ctttacgcac tgctttattc ataccaaact
     1801 tgtccaactc ttctatcatg ccgcccgggc cagattgcgg gttatggccg atactgcgtg
     1861 cggtcttggt catcaacccc atgtccagca tttcagtatt cgtccagaat gtcccgtcaa
     1921 ccatgatgca atccgcctgg ttcatcagtg gtggcaaatg tggctcaatc tctgccaacc
     1981 ctggtgaata ccagcatttt ttgccggtgg atatttcttc aatcaacaca ccgatggtgt
     2041 cgcccggatg aggatcattg cggtgtggag aatatggagg tgccgcactc ttcagtgcga
     2101 cagcggtaaa gcgcaagtta ggtacacccg ggatttcaaa actgtgggca ttgctgacat
     2161 cactggcatc aatagcgatt tcatggtggt taatgccaca gtaatgacca agaatattca
     2221 ggatctggtt accggtagtc aggtcttcgt ataccatctt tgtgcaatag atttcacgtt
     2281 tgacctgacc ttcacgcaac atgaacaagc ctgtggtatg gtcaatctgg gcatcaatca
     2341 gcacaatgcc ctggataccg gtatcacgta caccacgccc tggttgcaat ggggcaaact
     2401 cctggatttg ctgcaataca tcaggtgaag cgttaaacaa tacccaatcc acgccattac
     2461 tggaaacaca aattgatgat tgtgtgcgac gtttggcttt gatcgtgcct ttacgcaaac
     2521 catcacagtt ttgacaatta cagttccact gcggaaaacc accgccagcc ccagcaccta
     2581 atacgtgtat atgcataatc gctcgttatt caattcgttg attcatagtt gttgcaaaga
     2641 gtttcgagga ccatcataca acaataatgg atgactaccc tatgattgcc tgcacctcct
     2701 gcagcatgtc atctttcgat gcctgcttag taagactggc atcatacgca tgcgttgcat
     2761 gatcagcctg ctagagacaa tgattccctg ctcatgattt tcatgagcag gcatggcaag
     2821 tggattaaga gcataaaaaa accgcgctca taggcgcggt cttgtgctgc atttgtaaaa
     2881 ctattcctct gccgcacgct tctcacgacg tgtttttaca gcggtggcca gcgtttccaa
     2941 cacggcaacg gagtcttccc agccgaggca ggcatctgta atcgactggc catagttgag
     3001 gctgcaaccg ggtgagtgat cctggcggcc ttcgttcaaa tgcgactcta gcatcacgcc
     3061 aataatgcgc gcatctccac cagatacctg cactgcgaca tcgtcgacaa cagcaatctg
     3121 gtttttatat tgtttttggc tattcccatg actgcaatcc accatcaaca cgggcgccag
     3181 gccagcgtct gccagttgat cgcacactgc agccacactt tgcgcattgt aattcggggc
     3241 cttgccacca cgtaaaatca catggcaatc ttcattgcct ttggtcgcaa aaatggctga
     3301 ctcaccttct ttagtcatcg acaggaagtg gtgcggactt gctgccgttt taattgcatc
     3361 gatggcaact ttcaccccac catccgtgcc attcttgaac cccaccggac aagataagcc
     3421 tgaagcgagc tcacggtgaa tttgtgattc ggtggtgcgt gcaccaattg ccccccagct
     3481 gacgaggtca gccgtgtatt gtggtgacac cacatccaga aactccgtcg ccgccggtac
     3541 gccaatttcg ttcacatcca gcaaaaaacg gcgagctttt tcaaggcctg agttaatgtc
     3601 ataggtccca tctaagtgag ggtcgttgat caggcccttc cagcctactg tggtacgtgg
     3661 cttctcgaaa tagacgcgca tcacaatcaa caactcgccc gccaggcgat tacgcacatc
     3721 aagcaagcgt ttcgcgtatt ccatgccagc atcagtatcg tgaatggagc atggtccaat
     3781 aattaccagt aagcgatcat ccccttgatg caggatatga tgaatcgccg tgcgcgtttt
     3841 taggatattc tgcgtgctga ctgtactttc ttgtaggcgt gacaacagct ccgctggttt
     3901 taccagtggt ttgatctcca gcacccgtac atcatcggtc gctaattttt cgtatacagt
     3961 catgttgtta gaatcttttc cagcacttct agaaagcgcg cattttcgct aaataggcca
     4021 atgctcacgc gcaaatagtc tggcatatca taattggcca ccgggcgcac gatcacgcca
     4081 tgcttgagca aagcctggtt gacggcactg gcattggtca ctttaaaact gacaaagttg
     4141 ccaaaagaag gaatgtagct taaacccagt ttgttcaagc cttgtgtaac ctgggccata
     4201 ccggcctggt tcgccgcata actgcgtgcg acaaaatcct catcctgcag cgaaactgcc
     4261 gcagccactt gtgccaggct gttgacatta aatggctgac gcacacggtt catcaaatcc
     4321 gccaaggtcg catgcatgag gccaaaaccc acacgcaaac ctgcaaggcc ataagccttg
     4381 gaaaatgtgc gtgaaatgat cagattctca aactcagcta gccagccaat cgcttcagat
     4441 ttgtcggccg ctgaaagata ttcgtcatag gcttcgtcca ataccaccag gatatgtttg
     4501 ggcacttgac gcaaaaagtc cttcaaagct ggcttgctaa tcagcgtacc tgttgggtta
     4561 ttcgggttgg cgacaaatat caatttggtt ttagtggtga ttgcctttaa aaaagcaggc
     4621 aaatcatggc caaaatcaac agcgggaacc actacaccag tcgcgcctac ggcctgtgtc
     4681 accaatggat agacggcgaa cgcatgttgc gaataaatag cctcatcgcc agccgtcaag
     4741 aaggcgcgcg cagccaactc taggatatcg tttgaaccat tgccaaacac aatgtgtgcc
     4801 ggttgtacgc catatttctg gctaacggca tcccgcaaca caaaactatt gccatccggg
     4861 taacgcgcaa tctcataaat cgcctcttca actgccattt gcgccttggg actcatgccc
     4921 aatgggtttt cattcgaagc cagtttgacg atgtccgctt cattgaggcc catctcacgg
     4981 gctaattcac tgataggctt accaccttga tacggtgcaa tggcacgaat attggctggc
     5041 gctaattctg atagagagac agatgacgac ataggatgaa ttaaataata gcggttgggt
     5101 aagaacccaa cactttaagg aaagtggcgc gctcggccaa ctctttgagc gctgcctgta
     5161 ccgagggctg ctgttgatgt ccctcaatat cgacaaagaa aacataattc cagagattct
     5221 ggcgggatgg acgcgattcc agcttggtca tgctcactcc atggcgtgat aatggctcca
     5281 gcaactgtaa tactgcgcct ggcttattgt gtgcactcat caccaatgac gttttatcct
     5341 gtccagaagg ggctacagtg tggtttccaa gcaccaggaa acgtgtcgtg tttttcgggt
     5401 catcttcaat gttttcggca aggatattca ggtcgaacaa ttctgctgca cgcttgctgg
     5461 caatggctgc ggcaaatgtg ccctgtgtag cgaccagttc atgaatcatc tgggccgcac
     5521 gtgcattgct ggtcacggct tcgcgctcag cactcggcaa gatcttattc aaccattcat
     5581 gacattggga caaagactgt gcatgcgaga acacatgtgt aatctgttgc aggtcctgct
     5641 gcgctgataa caggcaatga tgaacaggta aagtcacctc tccgaccact tgcaacgcac
     5701 tacccagcaa taagtccagc gtaatgccca cagcaccctc ggttgaattt tccaccggca
     5761 ccacaccata atccgcctgg ccagcttcta ccgtacgaaa cacctcgtca atactgccgc
     5821 atacgaccgc ctggcgacct tcaccgaatt gcttcagtgc agcttcttcg ctgtatgtcc
     5881 ccaatgggcc cagaaatgct atggcaagct ctttttccag ggcacggcaa ttagacatca
     5941 cggcacggaa aatatggcta accgcctcag gggacagagg accatcattc tcagcctgca
     6001 agcggcggat aatctgtgct tcgcgctcag gacggtaaat gacaccgtca tctttcaaat
     6061 gcccgatttc acgtgcaagc ttggcgcgtt cattgaccag cgccaatact tgcgcgtcga
     6121 ttgcgtcgat tttatctcta aattgtttta atgaatcaga catttaaagt aatcaattaa
     6181 tgtatcttat gagttttttt gttcaaatgc ttgcatgaat tcaaccaatg cctgcacccc
     6241 ttcaatcggc atggcattgt agatactggc gcgcatgcca ccgacagaac ggtgaccttt
     6301 taactgcagc aagccctgcg tatccgccgc tttgagaaaa gcatcattca ggctttcatc
     6361 ccgcagacgg aatggaatat tcatgcgtga gcgatattct ttctgaatca ggttgtcata
     6421 aaactcggtt tgatccagat agtgatagag tagatcggcc ttagcaatat ttactttctc
     6481 aatggcatcc acaccgccct gggccagcag ccattcaaat accaaaccag caatgtaaat
     6541 gctataggtt ggcggcgtat taatcatgct ctggttatcg gcctgggttt tccaatgaaa
     6601 tacggcaggt gttaatggtg aagcctgctc aagcaggtct tcccgcacaa tcactaaaca
     6661 taaccctgcc gggccaatgt ttttctgcgc cccaccataa atcaccccgt attttgaaac
     6721 atcaatcggg cgtgacagga tatgcgaaga catgtccgca acaatgggca caccatgcgt
     6781 ctctggcaca tccagaaatt ccacgccatt gatggtttca ttagtgcaat agtgtacgta
     6841 ggcagcctct ttattcaact tccaatcggc aaatgtgggc accgtggtaa accctgtggc
     6901 ttcggcattg gcggcaatat taatctggcc ataggctttg gcatcctcta cgctgcgctt
     6961 actccaggag ccagtcacca catagtctgc agacttgcca ttgagcaagt tcatggggat
     7021 gatggcattt tcggcgatgg caccaccctg taaaaacaag actttgtaat tggccgggat
     7081 agatagcaac tggcgcaaat ccgcttcggt tttctccagg atactggtga actccttgcc
     7141 gcgatgcgac atttccatca cgctcatgcc agagccgtgc caatcgagca tttcagcctg
     7201 ggcacgttcg agtacgggtt ttggaagcac ggcggggccg gcagaaaaat taaaaatctg
     7261 tttcatgcgt ttatttattc ttcgctgtcg tcaccatcgt catcagtctc tacgattttc
     7321 tccagaccag acagtttctc accttcaccc aggttaatca gggtcacgcc ttgtgcagca
     7381 cgccccatgt cacggatttc cttgacgcgg gtacggatca acacgccgcc agtggtaatc
     7441 agcataattt catcttcagc tgtcaccagt ttggcagcaa ttgccaaacc gttgcgctca
     7501 ctgatcgcaa ttgctttcac gccttgtgta ccacgacctg aatgacggaa ctctgacaac
     7561 accgtgcgtt taccgtaacc attttcagtc gcaacgagta cagattgctg gtcattttca
     7621 gcaatcagca aggacaatac ctgctgtttg gccgccagtt tcataccgcg cacgccacgg
     7681 gttgcacggc ccatcgggcg gacatcatct tccgtgaacc agaccgcttt accaccattg
     7741 gaaaccagca cgatgtcgtt ctggccgttg gtgacttcag cgccaatcag atagtcaccg
     7801 tcatccaggt taatcgcgat aatgccggac ttacgtggat tagagaattc ggttaatgcg
     7861 gttttcttga ctgtaccctt ggctgtggcc ataaatacat aatggttttc gtcaaactct
     7921 ttcaccggca ggatggcgtt aattttctcg ccctcttcca gcgggaacag gttcacaatc
     7981 ggcttacctc ggctgacgcg gctaccttgt ggcacttcgt acaccttgag ccaatacaca
     8041 cggccacggc tactgaagca taggatgtaa tcatgggtat tcgccacgaa catcttgtcg
     8101 ataaaatcgt cttctttggt tgcagccgcc tgcttgccac ggccaccgcg tttttgggca
     8161 cggtattcat ccaaaggctg agatttgaca tacccagtat gtgataaagt cacgaccaca
     8221 tcttgtggcg taatcaggtc ttccagagac aagtcctgcg tattctgctc aatttcagaa
     8281 cggcgtttat cgccaaactg tttctggatt tcattgagtt cttccacaat aatcgcggtc
     8341 acacgctctg gcttcgccag gatatccagc aaatcagcaa tgatatccat cacttctttg
     8401 tattcgttga cgatcttgtc ttgctccagg ccagtcaagc gttgcaaacg catctgcagg
     8461 atttcctgcg cctgcacatc agacagtttg tagcctttag gggtgaggcc aaagtccaca
     8521 gacaagcctt ccggacgaga ggcttccatc gctgcacgct tgagcatttc ttcgaccact
     8581 ggcgaatgcc agctgcgtgc catcaattct tttttcgctt caggcggtgt tggcgcagct
     8641 ttgatgatgg caatcatctc gtccacgtta gctaatgcta cagccaagcc ttccaacacg
     8701 tgaccacggt cacgcgcctt acgcaattcg aatacggtac ggcgtgtcac cacttcacgg
     8761 cggtgacgca agaacgcctc taacatttgc ttgaggttga gcagacgcgg ctgaccatcc
     8821 atcagcgcca ccatgttcat accgaaagta tcttgcagct gggtctgctt gtagaggttg
     8881 tttaaaatca cctcaggcac ttcaccacgc ttgagttcga taaccacgcg catccctgac
     8941 ttgtcagact cgtcgcgtag gtcagaaata ccttcaatct ttttctcgtt gaccagctcg
     9001 gcgattttct caatcagtga cttcttgttt acctggtatg gcaattcatc aatgatgatg
     9061 gactggcgac cacctttttc caaatcttcg aaatgagtgc ggccacgcat caccacacgg
     9121 ccacgaccag tacggtagcc ttctttgacg ccagcggtac catagataat accggcggta
     9181 gggaagtctg gtgccggaat aaactcaatc agctcatcga cactgatttc cgggttcttc
     9241 aacaacgcaa aacaagcgtt aagtacttca ttgaggttat ggggtggaat gttggttgcc
     9301 atacccaccg cgatacctga actgccgtta atcagcaggt taggaatacg cgtcggcatg
     9361 atcaacggct cttgctcact accgtcgtag ttagggccga agtctaccgt ctctttttcg
     9421 atatccgcca acaactcatg cgcaattttg gacatgcgga tttcggtata acgcatcgcc
     9481 gctgcgttat cgccatcgac agaaccaaag ttaccctggc catctaccag catgtagcgc
     9541 aaggagaaat cctgcgccat acgcacaatt gtgtcataca cagcggtgtc gccgtgcggg
     9601 tgatacttac cgatcacatc accgacaata cgtgcagatt ttttataagg cttgttgtag
     9661 tcgttggaca gctcatgcat agcgaacaac acgcgcctat gcacaggctt gagaccatca
     9721 cgaacatcgg gaagtgcacg acccacaatc acgctcatgg cgtaatcgag atagctacgg
     9781 cgcatctcgt cttctaaact gataggcagg gtttctttag caaattgatc tggagactga
     9841 ctcattcttg acccaattta tagttaaaag ttatcacgcg attttagcat atttagcgcg
     9901 atcccgctcg ccgaatcgcg gttggtaagc aaatcacacg gcaaaaagct tactcgcatt
     9961 ccatcctgta aaaagaatag ggagccaagg ctccctattc taatgcgcag ccaaaccagc
    10021 agactcgcaa cacaaatcac aacgacatca acactatgtt cgcgtcagca attgcaaatc
    10081 tgccttcatg tcttcaatgt gctccaggcc gacggcaatt cgaaccagat tgtctttgat
    10141 accagcctca gccctggctt ccgcgctgac acggctatgt gtcgtcgttg cggggtgagt
    10201 gatggtagac ttcgcatccc ccaaattggc agtaattgaa atcaaccgcg ttgcatcaat
    10261 caacgcccaa gccgcttctt gtgcagtttg gtctgatcgc ggcttcactt caaacgtgac
    10321 aatacctcca cccaatcgtt gctgcttctg cgcaagcgca tattgcggat gcgatggcaa
    10381 acctgggtag tacacacgtt caacacctgg ctgctgctcc agccattgcg ccaatgccaa
    10441 cgcccgtgct gcatgcgcct ccatacgcac ctgcagtgtt tccagcccct taacaaacac
    10501 ccaggcattg aatgcactca tggtgacccc tgccgtacgt aaaaagccat aaacaggctc
    10561 gatcaaagcc ttactaccta acacggcccc acccagtacc ctgccttggc catcgatata
    10621 tttggtcgca gaatggatca cgatatccgc acctaattta agcggctgtt gcaaggcagg
    10681 cgtgcaaaag cagttatcta ccgccaggta agcgccaacc gcatgggcaa tctcagcaag
    10741 cttggcaata tcacccactt ctgtcagcgg attcgacggt gtctcggcaa aaaacaactt
    10801 ggtatttggt ttggcggccg ccttccatgc atcaacatcg gtcagtgaca ccaaagtcac
    10861 ttctagcccc cagcgcttga ggatattggt aaacaactgc accgtggtcc caaaaatact
    10921 gcgtgatgcc acaatatggt cgcctgcgtt acacagcccc atgacacatg ccaaaattgc
    10981 agacatgccg ctggctgtcg cgacgcattg ctacgcacct tccaatgccg ctagtttgtc
    11041 ctgaaacatg ctgaccgtcg gattggtaaa acgagaataa atatttcccg gctccgtgcc
    11101 gccaaagcgt gctgccgcct gcgctgcatt cttgaagcgg aaactggaat ttaaaaacaa
    11161 ggcttcagag ttctctccaa actctgtggt taatgtgccg gctcgcaccg ccagcgtttc
    11221 caaatggtat tgatcgttca tgtttcctca aacttctgtc cactatcgga cggcaataaa
    11281 aaacccgttt cagcattgcg ctaaaacggg tctttgggct ctcgctttag cagtatttat
    11341 aatgcgctcg caagctgagt ttggcttcgt ttaaagttcg ccactcaaat cagcgcaatt
    11401 tcattaaacg ccttttgcag gcgcctgtca agccttactc tacccccacc aaatctgtcg
    11461 ataacaggtt taaatccagc tgggtagtgg agttaggagg cagctgtttt ttgttcatgt
    11521 cactacgtgc agattcaata cggttcaagt aggccgcatc aatgtcaccc gtgacatact
    11581 tgccgtcaaa gcagctgcaa tcaaattcgg tcatatgagg attagacttg gttatacttt
    11641 cacgtagcgc gtccagatcc tggtaaatca aggcatccgc gccaatttcg gcacaaattt
    11701 cttcgtctgt gcgattggtt gccaacagct cgtgacggct aggcatatca atgccataga
    11761 catttggata gcgtacagga ggcgcagccg aagcgaaata taccttgtta gcgccagcat
    11821 ctcttgccat ctgaacaatt tgctgtgaag tcgtgccacg tacgatagag tcatccacca
    11881 gcaagacatt cttaccttta aactcgatgc cgatcgggtt tagtttttgg cgtacagatt
    11941 ttttacgcaa cgcctgaccg ggcataataa atgtgcggcc aatatagcgg tttttaataa
    12001 agccttcacg gtagtccaag cccaatgcaa ttcccaactg caacgcactt gggcggctgg
    12061 tatcaggaat agggatgacc acatcaattt gcttatctgc ccattcacgc ttgatttttt
    12121 ctgccagcaa tgtgcccata tccaaacggg tctggtacac ggatacacca tcaatcactg
    12181 agtcaggacg ggccaggtaa acatattcaa aaatgcagga ggtcagtttt gggttctcgg
    12241 cacattggcg gctgaataaa ttgccatcca tatcgataaa cacggcctct cctggctcga
    12301 catcacgcag catggtaaag cccagtacat ccagagccac gctctcggat gccacaatgt
    12361 actcggtacc tttatcggtc tcctgcttgc caatcaccaa cgggcgaata ccgtgcgggt
    12421 cacggaaggc cagtaaaccg aagttggcaa tcatggctac tacggcatat gcgcccttgc
    12481 aacgtcggtg tacgccagca accgcatcaa acaccatgtc actgtttagc aaagcacttt
    12541 ttgaagtcgc ttcaatttca tgcgccagca cgttcagcaa tacttcggag tctgaacttg
    12601 tattgatgtg gcgtagatcc tgcctgaaca tttctgactt taactgttcc gaattggtca
    12661 ggttgccatt atggccaagc acaatgccga atggggagtt cacgtaaaaa ggttgcgctt
    12721 ctgccgcgct agaagaaccc gctgttgggt aacggacatg cgcgataccc acattaccga
    12781 tcaagttacg catatggcgc gtatgaaaaa catccttcac caggccattg tttttgtgca
    12841 tgtggaaagt gttgccatca caggtcacga taccagctgc atcctgacca cggtgctgta
    12901 aaaccagcaa tccgtcgtaa agcaactgat tcaccgggtt cttgcctaca ataccgataa
    12961 ttccacacat agtgacatcc tatcaaacac ttgattacaa attaaatggc agactcgtct
    13021 acccgctggt ggtccaaatg cacatgctca gcaatactag atggcatcaa aggcagcaat
    13081 ttcaagacca gcgcctcaac cggcgaactt aacatggcat ttgtccagcg cgtatccttg
    13141 ggcagatcag tcatggcagc cagcattgcc agaatacata caatcagtac ccccttgcct
    13201 gccccagcga tgccgccaag taccctat
//
LOCUS       DATMSK010000235         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_370834,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000235 DATMSK010000000
VERSION     DATMSK010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370834"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /gene="motC"
                     /locus_tag="VNZ84_07815"
     CDS             complement(<1..340)
                     /gene="motC"
                     /locus_tag="VNZ84_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882287.1"
                     /note="homologous to MotA; this protein with a related
                     protein (a MotB homolog) forms the ion channels that
                     couple flagellar rotation to proton/sodium motive force
                     across the membrane and forms the stator elements of the
                     rotary flagellar machine; either MotAB or MotCD is
                     sufficient for swimming, but both are necessary for
                     swarming motility; these organisms have both MotA and
                     MotC; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HWU66901.1"
                     /translation="MDWGSLAGLLIALAGLVLGQVIDGGHLNSLMQPAAFAVVMLGTI
                     GAVLLQTELKLFIKGLRMLRLVFVHPLDDRRKLAIKINQWSQQARKESVLSLESYIKR
                     EQDPFVRKGLQ"
     gene            complement(340..1074)
                     /locus_tag="VNZ84_07820"
     CDS             complement(340..1074)
                     /locus_tag="VNZ84_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HWU66902.1"
                     /translation="MYTAKGTTNNTDQMLRQHSGLVKKMAYQLKAKLPSCVELDDLVQ
                     AGMIGLMDAIQRYEDTHGAQFETYASQRVRGAMLDELRGADWLPRGIRKNMRDIEVAV
                     QQLEQKLGRPPTESEIAKHMNFSLEDYYDVLSDCQGHQLIYYEDFQDDDGGEHFLDRY
                     VDDDSSDPVKSLLESDFREALIDSIDHLPDREKMLMGLYYEQELNLKEIGAIMNVSES
                     RVCQLHSQAVARLRASMRDRAWTGVA"
     gene            complement(1092..1880)
                     /locus_tag="VNZ84_07825"
     CDS             complement(1092..1880)
                     /locus_tag="VNZ84_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66903.1"
                     /translation="MFNYRDQADGLRRIMAKSSARIISVIGANGQPASPWMRNLAISM
                     LLPEQRLLLIHASRQDTIPYSLQAVTVKNSAIKRAITRHPQGYDLSCLIENDQLTSPL
                     SPDLKSQLDGIVRQLAYDYDTVMIETQLETQTFGLMLPVIAEHELVIQMDRSDTAIKA
                     AYVTIKHISQQHGNVPLSVVVTGANHDQGQQYFMRLNQVCKQFLGVALNFLGAIPAEE
                     TKPVAAAGRMQPGNVKPDQTALAFKSVAHHLEKQRITTPSMAVA"
     gene            complement(1873..3222)
                     /gene="flhF"
                     /locus_tag="VNZ84_07830"
     CDS             complement(1873..3222)
                     /gene="flhF"
                     /locus_tag="VNZ84_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HWU66904.1"
                     /translation="MNIRRFFGKNAREALAQVKQALGDDAINIANRSVNGGTEIMAML
                     EADVATTPGQASADTSMDNSRSLLDFVTAKDQPRQTTPQAMPTPATPATSQSSEEAAA
                     NESAIMDMLKQHSEQQEQAYQVATQTLEDKMMGMMQEMRQMRSHFETQMSAMTWQHHL
                     QHSPAKSKVLSTLLSASFSAALSRQIAEKMPNHIDVNKAPMWAKEVISRNLHTLDDED
                     ALLDRGGIYALVGPTGVGKTTTTAKLAARYVMKHGTQSLGLITTDSYRIGGYEQLRIY
                     GKILGVMVHAVKDEEDLKIALNELKNKHMILVDTVGVSQRDQAVTEQLSMLNRADSPI
                     QKLLCLNATSTGDTLTDVMRSYKKHDIAGCIVTKLDEAAAIGNVLDVLIRERMRLFYT
                     TSGQRVPEDIEVADKQALVERVLTPHQASLPYQYLDEELPLVISSMMQNAGQEQQHV"
BASE COUNT          740 a          875 c          859 g          918 t
ORIGIN      
        1 gttgcaaccc tttacgcaca aaaggatcct gttcgcgctt gatatagctt tccagagaca
       61 acacgctttc cttgcgggct tgctggctcc actggttaat tttaatggcg agttttcttc
      121 tgtcgtccaa tggatgcaca aagaccaggc gcaacatgcg caagcctttg ataaatagct
      181 tgagctcagt ttgaagcagg acggcgccaa tggtgcccag catcacaacg gcaaacgccg
      241 ctggctgcat caatgagttc aggtggccgc catcaatgac ttggcccagt accaaaccgg
      301 caagggcaat caggaggcca gccaggcttc cccaatccat caggcgactc cggtccaggc
      361 tctgtcacgc atggaagcgc gtaaacgggc aaccgcctgg ctatgcaact ggcacacgcg
      421 tgactcagac acgttcatga tagcgccgat ttctttcagg ttaagttcct gttcatagta
      481 caagcccatg agcatttttt cccggtctgg caaatggtcg atagagtcaa tcaatgcttc
      541 acggaaatca ctttccagta aggatttcac cgggtcgctg ctgtcgtcat cgacatagcg
      601 atccaggaaa tgctcaccgc cgtcgtcgtc ttgaaagtct tcatagtaaa tcagctgatg
      661 cccctggcag tcactgagca catcatagta atcttcaagg ctgaagttca tgtgcttagc
      721 gatttcggac tcggttggtg ggcggcccag tttttgttca agttgctgga cagcgacttc
      781 aatgtcgcgc atgtttttac ggatgccgcg tggcaaccag tctgcaccac gtaactcatc
      841 cagcatggca ccacgcacac gctgtgaagc ataagtttca aactgtgcac catgagtatc
      901 ttcgtaacgc tgaatagcat ccatcaggcc tatcatgccg gcctgcacca aatcatccag
      961 ttcaacacag gagggcagct tggcttttaa ttgataggcc atcttcttga ccaggccgct
     1021 atgctgcctc agcatctggt ccgtgttgtt cgttgtgcct ttagcggtat acatgttttt
     1081 cctcttctac attaggcaac cgccatagat ggcgtagtga tacgttgctt ttcgagatga
     1141 tgtgccacgg atttaaaagc caatgccgtc tgatccggtt tgacgttgcc gggctgcata
     1201 cggccagccg cggccacagg ctttgtttcc tcggcaggaa tcgcgccaag aaaattcagt
     1261 gccacaccca gaaactgttt gcaaacttga ttcaaacgca taaagtattg ttgaccttga
     1321 tcatgattgg cgcccgtgac taccacactc agcggcacat tcccatgttg ctggctgata
     1381 tgtttaatcg tcacataggc tgctttaatg gcggtatcgc ttctgtccat ttggatcacc
     1441 agttcgtgtt cggcaatcac cggcagcatc aagccgaatg tttgtgtctc cagctgtgtt
     1501 tcaatcatga cggtatcgta gtcatacgca agttgtctca ctataccgtc tagctgactt
     1561 tttaaatccg gagataaagg cgaggttaac tggtcatttt caatcaaaca gctcaagtca
     1621 taaccctgcg gatgcctggt gatggcacgt ttaatggcac tgtttttgac tgtaactgcc
     1681 tgcagtgaat aagggatggt gtcctggcgg ctggcatgta tcagcagcaa gcgttgctct
     1741 ggcagcagca tgctgattgc caggttacgc atccaggggc tggctggctg gccattggca
     1801 ccaatcacac taatgatacg tgccgacgac ttggccatga tgcggcgcaa accatcagcc
     1861 tggtcgcgat aattaaacat gctgctgctc ctggccagcg ttttgcatca tgctagaaat
     1921 caccaggggc agttcttcat ccaggtattg gtagggcagg ctggcttgat gcggcgtcaa
     1981 cacgcgttct accagtgctt gcttgtccgc cacttcaata tcttcaggta cacgctggcc
     2041 actggtcgtg tagaacaggc gcatacgctc gcggatcaat acatcaagta cattgccaat
     2101 ggcagcggct tcatccagct tggtgacaat acaacctgcg atatcatgtt ttttgtagct
     2161 gcgcatgacg tcggttaagg tgtcgccggt agaggttgca ttcaggcaca acagcttctg
     2221 aatcgggcta tccgcacggt tcagcataga gagttgctca gtgacggcct ggtcgcgctg
     2281 gctgacgcca acggtatcta ccaggatcat gtgtttgttt ttcaattcat tcagggcaat
     2341 cttcaggtct tcttcgtcct tgactgcatg caccatgaca cccaggatct tgccgtaaat
     2401 acgtaactgt tcgtaaccac caatccggta actgtcggtc gtgatcaggc ccaggctttg
     2461 ggtaccatgt ttcatcacat aacgggctgc cagcttggcc gtggtggtgg ttttacctac
     2521 accagttggg ccaaccaggg cataaatgcc gccacggtcc agcaacgcat cttcatcatc
     2581 tagcgtatgc aagttacggc tgatcacttc ttttgcccac attggtgctt tgttcacatc
     2641 aatatggtta ggcatttttt ctgcaatctg gcgggacaat gcagcgctaa agctggcaga
     2701 gagcagggtg ctcagcactt ttgatttagc tgggctgtgt tgcaaatgat gttgccaggt
     2761 cattgcactc atctgggttt caaaatgact acgcatttga cgcatttcct gcatcatgcc
     2821 catcattttg tcttccagtg tctgcgtggc cacctgataa gcctgctcct gttgctcgct
     2881 gtgttgtttc agcatgtcca taatcgctga ttcattggcg gcagcttctt cagaagactg
     2941 agaggttgct ggtgtggccg gtgttggcat cgcctgtggt gtcgtttgac gcggttgatc
     3001 cttggcagtg acaaagtcca gcaggctgcg gctgttgtcc atagaggtgt ctgccgaggc
     3061 ctggccaggt gttgttgcga catcggcttc cagcatggcc atgatttcgg tgccgccatt
     3121 cacactgcgg tttgcaatat tgatggcatc atcacctaat gcctgtttga cttgcgccaa
     3181 cgcttcacgt gcatttttgc caaaaaatct tctgatgttc ataattattg cgctccgatt
     3241 aaactggtga cacgaatgga tttgttgtct ggcagctcgt catgtgacaa cactctggga
     3301 tgcgggacag ttcttcttaa gaatctggat aaagcctggc gtagtggggc agccaccagt
     3361 aatactggtg tcaggcccat ttgctcttgt tg
//
LOCUS       DATMSK010000236         5021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_348020,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000236 DATMSK010000000
VERSION     DATMSK010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348020"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..2413
                     /locus_tag="VNZ84_07835"
     CDS             41..2413
                     /locus_tag="VNZ84_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWU66905.1"
                     /translation="MTASIFKLTPLQSALNVALCSLMLAASPSLVLAESAKTVTEKQH
                     YELGAGPLGHVLAEFAALTGTRLSFDPALVANLKSDGLKGSYTVSEGFDVLLKGSDFV
                     LADKGGQQYSLKKVAKVTSHASETSSGSEPVDTLAEVTVQGEKITRKLSETYSSVAVA
                     TAQDIRTHADQSLTEIMARTPGVYTQSRNETWGIRGVPVTGFDDQGPLTLNNAVSVFV
                     DGALQTSRMVTMSPLSTWDMKQVEIYRGAQSTVQGRNALAGAVIMQTNDPEFKNTLSL
                     QGNAGNYHQQGGALMANAALVPGVLAGRFALNVQNEDGYIRNDFLDKDADRRRSVNAR
                     GKLLWLAGEDTEALLTLAHTDYRTGSNGVTQINNQPQYYRLSQNTDARDHIQQNEATL
                     KLTHQLDKAWMLTSITSGTDANYQSLLDFDQRTNATQEADRHHQTRLFSQEFRANYQG
                     DDIKAHVGYYYGYSQIKFNDDLRVLGVGSALLARGKSQIRNHAIFGEINWDLDTQWQV
                     IAGLRYDREKNEAEVDYLRDVLGLSPVSGADGKKHFGALLPKAGINYIWSPTQRTGFV
                     VQRGYRGGGINTRVPSDHRPYDAEYTTTYEFSYRGSWPEYGFKTFANLYHTDWKDQQV
                     RQLDTATNSLYVSNAARSRMQGLEISAEYAINDAWQVIAGAAYNHTEYLDYINSDGLN
                     MRGKQFIMAPRTKINLGLNYHLGNQWLFNVDTVYQDSSPSVYLTTGTRRNDSVTLVNA
                     NASYRLNPHTTINAFVRNLFDREYVTNNTTGNVLDVGAPRLFGVALKLEI"
     gene            2546..4951
                     /locus_tag="VNZ84_07840"
     CDS             2546..4951
                     /locus_tag="VNZ84_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HWU66906.1"
                     /translation="MSHTCKPTVLAMLLASLATSAHAVELDIPSQPLDKALNALAHQT
                     GERIIFSTGLTDNKKAPALKGDYSVRQALDKLLNGSGLELKATPGGGYTLVKASPPEQ
                     SHAEPQLMPEVSVAGQVARERANAKMKGYVAKRNITATKTDSDILESPQSVSVVTRDQ
                     MTMQAVQSVGEALRYSAGIIAESNGPDPRADTIGVRGFSIGRDQFRDGLRNYAFSNQG
                     GTVIEPFGLERVEVLRGPSSILYGQGAPGGIVNLVSKRPTDKPLHEVGVVLGQYDRKQ
                     VNADFSDYLTEDGTWSYRIAGLVQDSDTQIDYVKDDRIYIAPSLTWRPSDFTTLTLLS
                     DYQKNDRGQGYQAWPRVGTLVNSRFGKVSSSTFLGEPGLDKYEQERYSLGYQFEHIFN
                     QTFTFRQNLRYQRMTTDAISTYLRALQADQRTITREAGGNEEDVDNLVVDSHLQAKWA
                     HGDFEHTVVAGIDSQRLNNDQRRTFALAGTLDIYAPVYGQTAINLVESANIEQRQRQT
                     GMYIQDQVKFDGKWVATLGGRKDWTRMETDNLRTPSASSSQKDQAETWRAGLVYLADN
                     GLSPYASYTESFLPVIGTTFAGAAFKPETGKQYELGLRFQPKESQSLYTVALFDLRRQ
                     NVTTTDLQHPTFNLQNGEVRSRGIELEAKTQWDAWSVIGSYTYLLPEITKSNNRNAQG
                     IAFEGKDPNHVPNHTAALWVDYRFPTALLQGMTVGGGVRYIGSRFGDDANTIRLPSYT
                     LVDLAVRYDLTQLGEGWKGWQFALNASNLFDKEYVSNCGYYGDGCRWGFRRNVMAQLN
                     YHW"
BASE COUNT         1225 a         1330 c         1384 g         1082 t
ORIGIN      
        1 caggccattt cattatttat acttaggaaa tcctcaggca atgaccgctt ctatttttaa
       61 actgactcca ttacaaagtg cattaaatgt ggccttatgc agcctaatgc tggctgccag
      121 cccatcattg gtcttggcag agagcgccaa gactgtcacg gagaaacaac attatgaact
      181 cggcgctggg cccttaggcc atgtgctggc cgagtttgcc gccttgacgg gcacccggct
      241 atcgtttgat ccggcactgg tggccaacct caaaagtgat gggctaaaag gcagttatac
      301 cgtgagtgag gggtttgatg tcttgctcaa aggcagtgac tttgtgctcg cagacaaagg
      361 cggccagcaa tactctctga aaaaagtggc caaagttacc agtcatgcga gcgaaacatc
      421 aagtggctct gaaccggttg ataccctggc agaagtgacg gtgcagggcg aaaaaataac
      481 ccgcaaactc agtgaaacgt attccagtgt tgcggtggcc accgcccagg atatccgcac
      541 gcatgccgac cagtcgctga ccgaaatcat ggcacgtacc ccgggcgtgt atacccagtc
      601 acgcaacgag acctggggta tccgtggggt accagtgacc ggttttgatg accagggtcc
      661 gctcaccctc aataacgcgg tgtcggtatt tgtggatggc gccttgcaaa ccagccgcat
      721 ggtcaccatg agcccactct ccacctggga tatgaaacag gtggaaatct accgtggtgc
      781 acagtccact gtgcaggggc gtaatgcctt ggcgggtgcc gtgattatgc aaaccaatga
      841 tccggaattt aaaaatacat tgagcctgca aggcaatgcg ggtaactatc accagcaggg
      901 cggggcattg atggcgaatg ccgccttggt gccgggtgta ctggcagggc gctttgcgtt
      961 gaatgtgcaa aatgaggatg gctatatccg caacgacttt ctggacaagg atgcggaccg
     1021 ccgccgctct gtgaatgccc gcggcaaatt attgtggctg gccggggagg acacggaagc
     1081 cttgctgacg cttgcacata ccgactaccg gactggcagc aatggtgtga cgcaaatcaa
     1141 taaccagccg caatattacc gtcttagcca gaacacggat gcgcgcgacc atatccagca
     1201 gaatgaagcg accctgaaac tgacgcatca actggataag gcctggatgc tgactagtat
     1261 tacgtctggt acggatgcca actaccagag cctgctggat tttgaccagc gcacgaatgc
     1321 gacccaggaa gcagaccggc atcaccagac gcgcctgttt agccaggagt tccgcgcgaa
     1381 ttaccagggc gatgacatta aggcacatgt cgggtattac tacggctact cgcagatcaa
     1441 gtttaatgat gacttgcgcg tgttaggcgt gggttcggcc ttgctggcac gtggaaaaag
     1501 ccagattcgc aaccatgcaa tttttggtga aattaactgg gacctggata cgcagtggca
     1561 agtgattgca ggcttgcggt atgaccgtga aaaaaatgag gccgaggttg attacctgcg
     1621 ggacgtgtta ggcctgtcac ccgtttctgg cgccgatggc aaaaaacact ttggggcgtt
     1681 gctgcccaaa gcaggcatca actacatatg gtcgcctacc caacgtaccg gttttgtagt
     1741 gcagcgcggt taccgtggcg gtggtattaa tacccgcgta ccttctgacc accgtcctta
     1801 cgatgccgaa tacacgacca cctatgagtt ttcctaccgt ggcagctggc cggaatacgg
     1861 ctttaaaacc tttgccaatc tctatcacac cgactggaaa gaccagcagg tacgccagct
     1921 ggatacggcg actaatagcc tctatgtatc caatgccgcc aggagccgga tgcagggcct
     1981 ggagatctcc gctgaatatg ccatcaacga tgcatggcag gtgattgccg gggcagccta
     2041 taaccatacc gaatacctgg actatatcaa tagtgacgga ctcaatatgc gcggcaagca
     2101 gttcatcatg gcgccgcgta ccaaaatcaa cctgggcctg aactaccacc tgggcaacca
     2161 atggttattt aatgtggaca cggtgtacca ggacagcagc ccatcggtat acctgaccac
     2221 tggtacccgc cgcaacgaca gtgtcacgct ggtgaacgcc aatgcttctt atcgattaaa
     2281 cccgcatacg acgatcaacg cgtttgtgcg taacctgttt gatcgcgaat acgtcaccaa
     2341 caatacaaca ggcaatgtgc tggatgtcgg cgcacccagg ttgtttggtg tggcgctcaa
     2401 actggagatt taaggtgctt gatcacggta ccggccggcc tgagccagtc ggcgccgcga
     2461 tgggcaaaat tttttaatgc agacatcaac atctttcact gcccaaacga cataccagta
     2521 taactacata accactggga aaaagatgtc acacacctgc aaacctaccg tactggccat
     2581 gttgctggcc agcctcgcca ctagtgcgca tgccgttgag ctggatattc catcgcagcc
     2641 gctggataaa gccctcaacg ccttggcaca ccaaaccggg gaacgtatta ttttctctac
     2701 cggcctgacc gataacaaga aagcccctgc actcaaaggc gactacagtg tacgccaggc
     2761 gctggataag ttattgaatg gcagcggcct ggagctcaaa gctacaccag gtggcggtta
     2821 tacgctggtt aaagcatccc ctcctgaaca aagtcatgcc gagccgcaat tgatgccgga
     2881 agtgagcgtg gccgggcagg tagcgcgcga gcgagccaat gccaagatga aaggctatgt
     2941 ggccaaacgt aatatcacgg ccaccaaaac cgacagcgat atcctggagt caccgcagtc
     3001 ggtctccgtg gtcacccgcg accagatgac catgcaggcc gtacaatctg tcggtgaggc
     3061 tttgcgttat agcgctggca ttatcgctga atccaacggc ccggatccgc gcgcagatac
     3121 cataggcgtg cgtggtttca gtataggccg cgaccagttc cgtgatggtt tgcgcaatta
     3181 tgcattcagt aaccagggcg gtaccgtgat tgagccattt ggcctggaac gtgtagaggt
     3241 gttacgtggg ccgtcgtcca ttttatacgg ccagggcgcg ccgggcggca ttgtgaacct
     3301 ggtcagcaaa cggccaacag acaaaccgtt acatgaagtt ggtgtggtgc tcgggcagta
     3361 cgaccgcaag caagtgaatg ctgactttag tgattacctg acagaagacg gtacctggtc
     3421 ttatcgtatc gcgggcctgg tgcaagatag cgacacccag attgactatg tcaaggatga
     3481 ccgcatttac attgcgccgt cattgacctg gcgccccagc gacttcacga cattgaccct
     3541 gctctcggat taccagaaaa atgaccgtgg ccaaggttac caggcatggc cgcgtgtcgg
     3601 cacactggtc aatagccgct ttggcaaggt gtccagcagc acgttcctcg gcgagccagg
     3661 cctggataaa tatgagcagg aacgctactc actgggctac cagtttgagc atatcttcaa
     3721 ccagacgttt accttccgcc aaaacctgcg ctaccagcgc atgaccacag atgcgatttc
     3781 cacctatttg cgggccttgc aggctgacca gcgcacgatt acgcgtgagg cgggtggcaa
     3841 tgaggaagac gtcgataacc tggtagttga tagccattta caagccaaat gggcgcatgg
     3901 tgactttgag catacggtcg ttgccggcat tgatagccag cgcctcaata atgaccagcg
     3961 ccgcacgttt gcgctggcag gcacactgga tatctatgcg cctgtctacg ggcagacagc
     4021 gattaacctg gttgaaagtg ccaatattga acaacgccag cgccagacgg gcatgtatat
     4081 tcaggaccag gtgaaatttg acggcaaatg ggtcgctacc ctgggtggcc gcaaggactg
     4141 gacgcgcatg gaaaccgata acctgcgtac gccatcagcg tcttcctcac aaaaagacca
     4201 ggccgagacc tggcgtgcag gcctggttta cctcgccgac aatggcctgt caccctatgc
     4261 gagttatacc gagtcttttc tgccagtgat aggtacgacg tttgcggggg ctgcgtttaa
     4321 accggaaacc ggcaagcagt atgagctggg cttgcgtttc cagcctaagg aaagccagag
     4381 tctatatacc gtggccttgt tcgatctgcg ccgacagaac gtgaccacaa ctgacctgca
     4441 acatcctacg tttaatctgc aaaatggtga ggtgcgttca cgcgggattg agctggaggc
     4501 caaaacccaa tgggatgcct ggtctgtgat cggtagctat acctatctcc tgcccgagat
     4561 caccaaaagc aataaccgca acgcacaagg cattgcgttt gaagggaagg atcctaatca
     4621 cgtgcctaac catacggcag cgttgtgggt agattaccgt ttcccgactg ccttgctgca
     4681 aggcatgacc gtcggtggtg gtgtgcgtta cataggcagc cggtttgggg atgatgcgaa
     4741 taccatccgc ttgccgtctt atacgctggt ggatttggcg gtacgttacg acctgacgca
     4801 actgggagaa gggtggaaag gctggcaatt cgcgctcaat gccagcaacc tgtttgataa
     4861 agaatatgtt tcaaactgtg gctactacgg tgatggctgc cgctggggct tccgccgtaa
     4921 cgtcatggca caacttaact atcattggta atagacaagg ttcatgcgac acatattggt
     4981 actttggcat cgctgggtag gcctggcgat ggccgctttc c
//
LOCUS       DATMSK010000237         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_88749,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000237 DATMSK010000000
VERSION     DATMSK010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88749"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..702)
                     /locus_tag="VNZ84_07845"
                     /pseudo
     CDS             complement(1..702)
                     /locus_tag="VNZ84_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881534.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(794..1780)
                     /locus_tag="VNZ84_07850"
     CDS             complement(794..1780)
                     /locus_tag="VNZ84_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWU66907.1"
                     /translation="MNPKHEDMIRQAAARWYVRMREVAPDSPERTTFEVWLLSDRRHQ
                     VAYQTIEATMDDFSSTARLKELSNALSQKLYFEQTARRKKISKLGSGLAVMLVCVGLG
                     LLGRTQYVQWQAAPLSSEMQTTAVAQMLKRTLEDGSVVTANANSAMEIVFYRHQRLVK
                     LSRGEAIFEVTKDPERPFVVETPQAKVTVLGTRFAVNQLSKKVRISVDHGRVQVLRKD
                     GYGQALILGNGEVAEIEHDAPHKVNRNAADSFSFTSGRIIFDRADMFEVADTLSRYRN
                     PTVKAVFFGDQTPKVNAVLKVSEVESFIQTLPQSVPVALQHRGDTLVLAPNR"
     gene            complement(1780..2301)
                     /locus_tag="VNZ84_07855"
     CDS             complement(1780..2301)
                     /locus_tag="VNZ84_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWU66908.1"
                     /translation="MDKTMVWCGINLQWAYTDLLGYIRFKVTCPHLAKDILHDALIRY
                     TVSSSANKDAEPHAYMRSIAKNLIVDSYHHAQRFISLEAELGLNREWLDTPTLSVEQL
                     SDIKQRLEMIQQILEQLPPRCRQVFWMYRVEGHTQTEIAAALGISKNMVERHVMRAII
                     DLSSARELIEVAL"
     gene            complement(2420..>2871)
                     /locus_tag="VNZ84_07860"
     CDS             complement(2420..>2871)
                     /locus_tag="VNZ84_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66909.1"
                     /translation="QTTFGDNQVNLATQYDLIDDGYSFFIVFGTFSRADQSTTLNGTF
                     DISLLVDDLNIPDPWLHPDIPVPVFANFYAEESFIDPYSGEIIQQTILSGTGKGLMSQ
                     REYLSFTINWSLFTLEPMSPVPSPPSSWMFVIGLLLIGSIRICKRAE"
BASE COUNT          703 a          712 c          711 g          745 t
ORIGIN      
        1 catatcccaa ttcacaacat cgccaaatgg ttcgttaacc cgaatgccgt caataaagac
       61 acttaaacct tgtggcgtac caatctgcgg gccagcggta aaaccacgat attgcacatc
      121 catctggaac gggttgcctt gatagtcatt cactgtgacc gattgcaact tacggttcat
      181 caagtctgta atactcaatg aatgcgcttc tttaatctct ttggcagaga tactttggac
      241 attgccgggg atttcttctt tagtaaggcc ggtgcctccc aaagggccga cttcataaaa
      301 acgcttggcg cggacttcga ctttgtcgag ctgctaatcc tttgcttctg cttttgttac
      361 acgttgaatc agtatttttt caccctcaac cttgccttgc agtccactac catccagcaa
      421 tcgtttgatg gcttctttac gtgtcatgcg gcctttaacg gcgggggatt ttttgtcgac
      481 gatgagtgcg ctatctaccg tgagtgataa attgctctga atggcaaact ggcgtagtga
      541 agcagccagt ggttgggctg gcaggtctat attcagcggt aaggcaaagt tctcttcttg
      601 ctcgccgggc aatgccgtat tcaactcagc atgaagcgct gccggtatat tcaggccaat
      661 gagtgcggcc agtgcggctg ttttaatcgt tatattttgc atgagtcatc cgtcagttac
      721 aggtcttgtt aaagaggtaa ctgacagacg ggtgaaatgc ctgatatgcg taacttaaaa
      781 gaaatgtaaa gagttagcgg ttgggtgcaa gcaccagcgt atcgcccctg tgctgcaagg
      841 cgaccgggac cgattgcggc agggtttgaa taaagctttc gacctcactc accttgagta
      901 ctgcattgac tttgggcgtc tggtcgccaa agaataccgc tttgaccgtt ggattacggt
      961 aacgcgacaa ggtatcggcg acttcaaaca tgtcggcacg gtcaaaaatg atgcggccac
     1021 tggtaaagct gaaactgtcg gccgcattgc ggttaacctt atgcggtgcg tcatgttcaa
     1081 tctcagccac ttcgccattg cccaggatca atgcctgtcc ataaccgtct ttgcgcaaca
     1141 cctgtacacg gccgtggtct acactgatac gtactttttt actgagctgg ttgaccgcaa
     1201 aacgcgtgcc caatacggtg accttggctt gcggggtttc aaccacaaat ggccgctcgg
     1261 ggtctttggt gacttcaaaa atagcttcac cgcggctaag tttgaccagg cgctgatggc
     1321 ggtagaacac tatctccatg gcgctattgg cattggctgt gaccacagag ccatcctcca
     1381 gtgtgcgttt aagcatctgt gcgaccgccg tggtttgcat ttcactcgag aggggcgcgg
     1441 cctgccactg tacatactgg gtgcggccca acaaaccaag accgacacag accagcatca
     1501 cagcgaggcc agagcccagt ttggagattt ttttgcggcg tgcggtttgc tcaaaataga
     1561 gtttttgcga gagcgcatta gaaagctctt tgagccgggc ggtggagctg aagtcatcca
     1621 tcgtggcttc aatcgtctgg taggccacct ggtgtctgcg gtctgacaat agccagactt
     1681 cgaatgtcgt ccgctcaggc gagtctggcg ccacttcacg catgcgcacg taccagcgcg
     1741 cagccgcttg cctgatcatg tcctcgtgct tagggttcat tagagtgcaa cctctatgag
     1801 ctcacgcgca ctgcttaaat caataatggc ccgcatcaca tgccgctcga ccatgttttt
     1861 gctaatgccc agtgcagcgg caatctcagt ttgggtgtga ccttccacgc gatacatcca
     1921 gaacacctgg cgacaacgtg gtggtaattg ctccagaatc tgctggatca tctccagccg
     1981 ctgcttgata tcactgagct gttccacgga caaggtcggc gtatccagcc attcgcgatt
     2041 aaggcctaat tcagcttcaa gggaaataaa ccgctgtgcg tgatgatagc tgtcaacaat
     2101 caggtttttg gcaatgctgc gcatgtaggc gtgcggttca gcatctttgt tggcactgct
     2161 ggaaacggtg tagcggatta aggcatcatg caggatatct ttggccaggt gcgggcaagt
     2221 gactttaaag cgtatgtaac cgagcaaatc ggtatacgcc cactgcaaat tgatgccaca
     2281 ccaaaccatt gttttatcca tgctccttat tggcaaaata catgccatcc aatttgcgag
     2341 ctaaaaaagt ggtaatctcg ccttcttcta aaaaacctga ggcatttcag ccaccaactt
     2401 ggctacatac aaccaatttt cactcggcgc gcttgcagat tcttatgcta cctattaata
     2461 acaagcctat gacaaacatc catgagcttg gcggggaggg gaccggactc ataggctcaa
     2521 gcgtgaaaag tgaccagttg atggtgaagg aaaggtattc acgctgtgac attagccctt
     2581 taccagtccc acttaaaata gtctgttgga taatttcgcc actataggga tcaataaatg
     2641 actcttccgc ataaaagttt gcaaacacgg ggaccggaat gtcagggtga agccaaggat
     2701 ctgggatgtt caaatcatcc acgagcaagg aaatatcaaa agttccattt aaagtggttg
     2761 actgatctgc gcgactaaat gtaccgaaaa caataaaaaa actgtagccg tcatcgatca
     2821 gatcatattg cgtagcgagg tttacttgat tatccccaaa cgttgtttgt c
//
LOCUS       DATMSK010000238        14013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_229688,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000238 DATMSK010000000
VERSION     DATMSK010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14013
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229688"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1155)
                     /gene="pyrC"
                     /locus_tag="VNZ84_07865"
     CDS             complement(121..1155)
                     /gene="pyrC"
                     /locus_tag="VNZ84_07865"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWU66910.1"
                     /translation="MTTELTLIRPDDWHLHLRDGDGLKAVLPDTARQFARAIVMPNLR
                     PPVTTAEAALAYYQRIKQAAPAGLVFEPLMTLYLTDNSPAQEIAAARASGVVQAVKLY
                     PAGATTNSDSGVTSLHKCAAALAEMEKQGMPLLIHGEVTDGDVDVFDREKVFIERHLQ
                     PLLKDFPGLKVVFEHITTKDAAEFVAAGPDTLGATITAHHLLMNRNAMFTGGIRPHHY
                     CLPVLKRETHRQALVKAAISDSGKFFLGTDSAPHPKSAKEASCGCAGMYTAHAAIELY
                     AEAFEEAGALDKLENFASVYGSDFYGLPRNTDKITLRKESWLVPESIPFADDVLVPLR
                     AGQTIAWRLM"
     gene            complement(1167..2021)
                     /gene="rsmI"
                     /locus_tag="VNZ84_07870"
     CDS             complement(1167..2021)
                     /gene="rsmI"
                     /locus_tag="VNZ84_07870"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWU66911.1"
                     /translation="MSEIGTLYVVATPIGNLGDITQRALDTLKQVDAIAAEDTRHSVG
                     LLRHFGISKPLLAVHEHNEQQSAQGLIKRLLVGESIALVTDAGTPAVSDPGAVVVQAV
                     RQAGVKVVPVPGASAVITALSASGIIQPGFYFEGFLPASGSQRRKRLQLLKTIPSTLV
                     FYEAPHRIVECVQDLQDVLGGVRQLTLARELTKTFETIHTCLLTEAVDWLKADLNQQR
                     GEFVLLVHPDIQEKAEGLDEEALRILQRLLQELPLKQAVALATDITGHKKNELYETAL
                     ALKAGNSQ"
     gene            2102..2458
                     /locus_tag="VNZ84_07875"
     CDS             2102..2458
                     /locus_tag="VNZ84_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWU66912.1"
                     /translation="MKLNQNNQGLAAENAAALFLQQQGLSLVMQNYSCRYGEIDLIMR
                     EGKTLVFIEVRLRSNQAFTSAADSIDRRKQQKLIRAAQFYLQNQDLNCPCRFDAILFD
                     NTLYQSPNWIRNAIDT"
     gene            2537..3121
                     /locus_tag="VNZ84_07880"
     CDS             2537..3121
                     /locus_tag="VNZ84_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWU66913.1"
                     /translation="MTQHMFTRKISAIALLLISTQLSGCFPAVVGGAAAGGAMAADRR
                     TSGIYVEDENIELKTLKRMSQYMDKASHINVTSYNRIVLLTGEVPNEGQQTQAETLTR
                     EISNVRNIHNALTVGPASSIGDRSNDTFLTTKVKARFVSENLFPANVVKVVTEASVVY
                     LMGIVSEKEANDAVEIARTTEGVSKVVKVFEYTN"
     gene            3151..3441
                     /locus_tag="VNZ84_07885"
     CDS             3151..3441
                     /locus_tag="VNZ84_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HWU66914.1"
                     /translation="MENQEIIIQGITLAGKPFRPSDWVDRMCSTYATFGDDKKLRYSP
                     YLKPKLVNNVRSLSVDMKLKDVNPAGFEQLMQFAKENQLSVISPAGEAVTIE"
     gene            complement(3446..4051)
                     /locus_tag="VNZ84_07890"
     CDS             complement(3446..4051)
                     /locus_tag="VNZ84_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWU66915.1"
                     /translation="MLKVEITKRDKASIATVFQHNQVFSVYGIHMGRIFNKNKGWQRT
                     FGLFAILLAVFSLGVAETSLAADSRWLMQDLAGRTHQLAQYKGRWVVVNYWAPWCPPC
                     LEEMPELVTFYDAHAKEVMVLGVAVQYKTEQSVRNYIEDMLISYPIILGDQQKHLPAT
                     PEVLPTTYIYAPDGSLYKIKRGALSKVWLESLLKATAPEAK"
     gene            4152..4478
                     /gene="trxA"
                     /locus_tag="VNZ84_07895"
     CDS             4152..4478
                     /gene="trxA"
                     /locus_tag="VNZ84_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HWU66916.1"
                     /translation="MSDNITHLSDASFEQEVLQSTIPVLVDYWAEWCGPCKMIAPILD
                     DISKEYAGRLKVAKLNIDDNQNTPPKYGIRGIPTLMLFKNGNVEATKVGALSKSQLSA
                     FIDSNI"
     gene            4559..5818
                     /gene="rho"
                     /locus_tag="VNZ84_07900"
     CDS             4559..5818
                     /gene="rho"
                     /locus_tag="VNZ84_07900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWU66917.1"
                     /translation="MHLSDLKHLPVTELVEMAIANDIENASRMRKQDLIFAILKNKAK
                     KGDSIFGDGTLEVLPDGFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDSIQGEIR
                     TPKDGERYFALVKVDSVNGEAPENTKHKILFENLTPLFPTVPLTLEREIRGEENITSR
                     VIDMIAPIGRGQRALLVASPKSGKTVMLQNIAHAITANHPDCVLIVLLIDERPEEVTE
                     MTRSVRGEVVASTFDEPATRHVQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VIPSSGKVLTGGVDANALQKPKRFFGAARNVEEGGSLTIIATALVDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMAEKRIYPAINVNKSGTRREELLLDKDVLQKIWVLRKLLYPMDD
                     LEAMEFLLDKIKGTKSNNDFFDSMRRG"
     gene            5923..6123
                     /gene="rpmE"
                     /locus_tag="VNZ84_07905"
     CDS             5923..6123
                     /gene="rpmE"
                     /locus_tag="VNZ84_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWU66918.1"
                     /translation="MKADIHPNYPEINVTCSCGNKFKTRSTNGKDLNIEVCSQCHPFY
                     TGKQKIVDTAGRVEKFRQKYGM"
     gene            6241..7920
                     /locus_tag="VNZ84_07910"
     CDS             6241..7920
                     /locus_tag="VNZ84_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HWU66919.1"
                     /translation="MQFYIDHDWQENMATPRAKVGERAKTHLLILLCAIWLCVGLIGH
                     SPWKPFESQSASIIQNLIDHPFLSSSSWIHGDWIAPTSAGNPTLETPPLFYWTATGFA
                     HLLSPLLPTHDAARLSVGLWMLLTLIMTGLSGRELWNLGVGRQTNFIFIGCLGLVVSA
                     HTMMPAIAALSGITMAFYALALIMRKPVRAMWLLCTGLLVAFLSGGVVPALTIILSVT
                     CLLTLPAIRQQKHVYRAMIAGTLFALPGIYLWCWLCQQWAPGLWQSWWQAQWQFQLPG
                     NHGYFLRTLAWYAWPALPFALWGIWRYRHSVLNTYRFQLGLIFFGIAFILIGLLSDRS
                     EVSALPLLVPLTVLAAGSIETLKRGAAGALNWFGLILFGILISIAWLGWWAMLNGTPV
                     KLYQRLSYLSGLETIPFSLLYGAIAVAVTLIWLSIVLRSKHSNRSSATNWAIGMTCAW
                     TVFMSLWLPMIEAARTYQPLFSDLRKHLPAHYNCIHAQNIGTSQADLLHYHAGVKLEY
                     HAPGATTQCDLYLIEDQPGKRHALPGERWHVIWEGEQTRQNKESFRLLQRR"
     gene            complement(8001..8219)
                     /locus_tag="VNZ84_07915"
     CDS             complement(8001..8219)
                     /locus_tag="VNZ84_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HWU66920.1"
                     /translation="MEVDKLIKMANQIGDFFEANPDVEEAKREIASHLKKFWNSIMIK
                     TLVAHVQQHQGQGLHPKVIAAIQQHVHL"
     gene            complement(8236..9147)
                     /gene="fdhD"
                     /locus_tag="VNZ84_07920"
     CDS             complement(8236..9147)
                     /gene="fdhD"
                     /locus_tag="VNZ84_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWU66921.1"
                     /translation="MLIEVVEGEDCALPASSEWQVIPAERAVSTLTVTRWREQQSTVV
                     TDALAQEVPVALVYNGISHVVMLATPADLEDFAYGFSFTEGIITESSQIYGVEAKALM
                     QVDGQLQGIELHIELATQQFVALKAQRRNLTGRTGCGLCGAESLQQVFKQPMVGGEPL
                     VSLSLCAIEAALDALRLKQPLQQLTGATHASAIVDAQGQVVSVREDVGRHNALDKLIG
                     ALLRQGQDFSRFWSGHWVLTTSRASYEMVQKVAVCGGRSLVALSAPTALAVSLAQKYD
                     FLLVGFAKTTQCVVYSGELAAHREHES"
     gene            complement(9147..12038)
                     /gene="fdhF"
                     /locus_tag="VNZ84_07925"
     CDS             complement(9147..12038)
                     /gene="fdhF"
                     /locus_tag="VNZ84_07925"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HWU66922.1"
                     /translation="MDDIKYSSSQPPVLKYDPNTDYGTPKSDSATMVTLNIDGVDVTV
                     PDGTSIMHAAQLGGVTVPKLCATDSLEPFGSCRLCLVEIEGRRGYPASCTTPVAPGLK
                     VKTQTPKLADIRRGVMELYISDHPLDCLTCGANGDCELQDMAGAVGLREVRYGYEGEN
                     HLGQSKDESNPYFTFDPSKCIVCSRCVRACEETQGTFALTIQGRGFESKVSAGNKDFL
                     DSECVSCGACVQACPTATLMEKTVIEAGTPEHKVTTTCAYCGVGCSFDAEMKGEQVVR
                     MTPSKEGGANHGHSCVKGRFAWGYATHADRITTPMIRKSIHDPWQKIGWEEAINYAAS
                     EIKRIQAQYGKEAVGGITSSRCTNEEVYVTQKLIRAVFGVNNVDTCARVCHSPTGYGL
                     KQTLGESAGTQTFDSVMKSDVIFVMGANPTDGHPVFGSLMKRRLREGAKLIVVDPREI
                     DLVDNSPHVRADYHLKLRPGTNVAMISAISHVIVTEGLVQEGFVKARCEWDSYVAWRD
                     FAAKPENSPEALEKELGVSANVVREAARLYATGGNAAIYYGLGVTEHSQGSTTVMGIA
                     NLAMATGNIGREGVGVNPLRGQNNVQGSCDMGSMPHEFPGYRHVSDDETRAQFEQAWG
                     VTLSADPGLRIPNMLDLAAEGSFKAVYCVGEDIAQSDPDTQHVTHALESMECVIVQDL
                     FLNETAKFAHVFFPGASFLEKNGTFTNAERRISPVRKVMTPKNGMEDWEITAKFSEAL
                     GYPMHYAHASEIMDEIAALTPTFTGVSFKKLDELGSIQWPCNDEAPQGTPTMHVDEFV
                     RGKGKFFITQYVPTTERVNQKYPLILTTGRILSQYNVGAQTRRTHNVAWHHEDVIEIH
                     PHDAEDRGIAEGDWVGITSRAGQTVLRAKITERVQPGVVYTTFHHPESGANVITTDNS
                     DWATNCPEYKVTAVQVSRVNQLSDWQQHYRAFSDTQIELSGMDPQTVVS"
     gene            complement(12059..13624)
                     /locus_tag="VNZ84_07930"
     CDS             complement(12059..13624)
                     /locus_tag="VNZ84_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWU66923.1"
                     /translation="MVKVYVPTDSTALSLGAERTAKKIAQEAQLRGVQVELVRNGSRG
                     LFWLEPLVEVETAQGRVAFGPVQPGDVPGLFDAEFTNALAEKAKAHALYLGLTDELAW
                     LKKQQRLTFARVGIVDPLSLEDYLAHDGYKGLKNALSMSGAEIVKAVTDSGLRGRGGA
                     AFPTGIKWNTVLNAPAEQKYVVCNADEGDSGTYSDRMVMEDDPFVLIEGMTIAGIAVG
                     ATQGYIYLRSEYPHALKTLNEAIRKANLAGYLGENILGSSHTFHLEVRRAAGAYVCGE
                     ETSLLESLEGKRGLVRFKPPLPAIEGLFGQPTIVNNVISLATVPIILDKGAQYYADYG
                     MGRSRGTLPIQLAGNLKQTGLVELAFGATLRELLYDYGGGSASGRPIRAVQVGGPLGA
                     YLPESQFDTPLDYEEFTKIWAVLGHGGIVAFDDSVDMAKMARYAFEFCAEESCGKCTP
                     CRIGSTRGVEVMDKIVSGKQHQKNVQLLRDLSDTMLNGSLCALGGMTPYPVLSALNHF
                     PEDFGLTNKEAAA"
     gene            complement(13617..>14013)
                     /locus_tag="VNZ84_07935"
     CDS             complement(13617..>14013)
                     /locus_tag="VNZ84_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HWU66924.1"
                     /translation="PLLHAIQDDIGYVPETVYPEIAKALALSVAEVHGVVSFYHHFRT
                     HPIGKHVLQVCRAESCQSMGSEKLEAALKAKLGVDYHQTTADGNVTLLPVYCLGNCGC
                     SPAIMLDDEVHGRMNADKVADLIAEVCHG"
BASE COUNT         3581 a         3835 c         3191 g         3406 t
ORIGIN      
        1 tgttcgcccc gcatttcatc tatcgtcacg gcaaatgcgt gtgcacttaa catcatactg
       61 aatcctgcaa ccaaccattt cttgttcatc gcttattctc caatgtcaga atgaaatttt
      121 ttacatcaat ctccaagcaa ttgtttgtcc ggcacgcaaa ggcaccaata catcatctgc
      181 aaacggaata ctctctggca cgagccagga ttccttgcgc agtgtgattt tgtcggtatt
      241 acgcggcagg ccgtaaaaat ctgacccata aacactggca aagttttcca gcttgtccag
      301 tgcaccagcc tcttcaaatg cctcggcata caactcaatc gctgcatgtg cagtatacat
      361 gccagcacag ccacagctag cctccttggc agacttggga tgtggcgcac tgtcggtgcc
      421 aaggaagaat tttccggaat ccgaaatagc cgctttcact agggcctgcc tatgtgtctc
      481 acgcttcaat accggtaagc aataatggtg cgggcggata ccgccagtga acatcgcgtt
      541 acggttcatg agcaggtgat gcgcagtgat ggttgcgccc aatgtatcag ggcctgcagc
      601 gacaaactca gcggcatcct tagttgtaat atgctcaaac accactttca ggcctggaaa
      661 gtcctttaat agcggctgca aatgacgctc gataaacacc ttctcacggt caaacacatc
      721 gacatcgcca tcagtcactt caccgtgaat cagcaaaggc atgccctgct tctccatctc
      781 agccaaagcg gctgcacatt tatgcaaact ggtgacacca gaatcactat tcgtcgtcgc
      841 accggcagga tacagcttta cggcctgtac gacgccgctc gcacgggcag cagcaatctc
      901 ctgggcgggc gaattatcgg ttaaatacag cgtcatcagt ggctcaaata ccagcccagc
      961 cggtgcagcc tgcttgatgc gttgataata ggcgagtgct gcttctgccg tagtcactgg
     1021 cggtcgcaag ttgggcatca caatagctcg tgcaaattgt ctggctgtat ctggtagcac
     1081 ggccttcaag ccgtcaccat cccgcagatg caaatgccag tcatcagggc gaatcagggt
     1141 taattcagtt gtcattccat taccttttac tgtgagttgc cggccttgag cgccagggcc
     1201 gtttcataca attcattttt tttgtggcca gtgatatccg ttgccagcgc caccgcctgt
     1261 ttgagtggaa gctcctgcaa caatcgctgc aagattctta atgcctcttc atctagccct
     1321 tctgctttct cctggatatc aggatgcacc agcaacacga actctccacg ctgctgattg
     1381 agatcagcct ttaaccagtc taccgcctct gtcaataggc aggtgtgtat ggtttcaaac
     1441 gttttggtca gctcacgcgc cagggtgagc tgacgcacgc cacccaacac atcttgtaaa
     1501 tcttgcacac actcaacaat ccggtgcggc gcctcgtaaa ataccagcgt agaagggatc
     1561 gttttcagca attgcaatcg cttgcgccgc tgcgaaccac tcgcaggcaa aaaaccctcg
     1621 aaataaaaac ctggctggat aatgcctgac gctgataaag cggtaatcac tgcactggcg
     1681 ccgggcacag gcacgacttt cacaccggcc tgccgcaccg cctgtaccac gactgcgcca
     1741 ggatcgctca ccgctggggt gccggcatcg gtgaccaaag cgatcgactc ccctactaac
     1801 agccgcttga tcaagccttg cgctgactgc tgctcattat gctcatgcac ggccagcagc
     1861 ggtttgctaa tgccaaaatg ccgcaacagg cccacactat gcctggtgtc ttcggcggca
     1921 atcgcatcca cctgctttaa cgtatccaat gcccgctggg taatatcccc caggttacca
     1981 atcggcgtgg cgactacata taatgtacca atttcgctca tgctggcatt ttatgccaac
     2041 acactggttt cgctaaatat tattcattag actgacggag aaatccggcc acagaacata
     2101 catgaaactt aatcaaaaca accagggcct ggccgcagaa aatgcggctg cactgttttt
     2161 gcagcagcaa gggctttctc tggtcatgca aaactatagt tgccgctatg gtgaaattga
     2221 cctcatcatg cgtgaaggaa aaacgctggt ctttatagaa gtacgcttgc gtagcaatca
     2281 ggcattcacc agtgcggcgg atagcataga ccggcgaaag caacaaaaac taatccgcgc
     2341 agcccagttt tacctgcaaa accaggactt aaattgcccc tgccgattcg acgccatctt
     2401 gtttgacaac acgctttacc agtcgccaaa ctggattcgt aatgcaatcg acacataaaa
     2461 agcgtagaat tttatttatt gcctgctgtc acacctaaca tctttcagtt ttttctcgca
     2521 gatccaagga agcttcatga cccaacacat gttcactaga aaaatttctg ccattgcctt
     2581 gttattgatc agcacacaat taagtggctg tttccccgca gtcgttggtg gcgcagcagc
     2641 cggcggcgcc atggcagctg accgccgtac ttctggcatt tatgtcgaag atgaaaacat
     2701 tgaactcaaa acccttaaac gcatgtccca atatatggac aaggcctcgc acatcaatgt
     2761 caccagctat aaccgtattg tgctcctaac aggtgaagtg cctaacgaag gccagcagac
     2821 ccaggctgaa acacttaccc gtgaaatctc caatgtccgc aacatccaca atgcactgac
     2881 cgtaggtccg gcatcatcta tcggcgaccg cagcaatgac actttcctga ccaccaaagt
     2941 aaaagcccgc tttgtcagtg aaaacctgtt ccctgccaat gtggtgaaag tcgtgactga
     3001 ggcaagcgtg gtttacctga tggggatcgt ctctgaaaaa gaagctaacg acgctgttga
     3061 aattgccaga accacagaag gcgtttccaa agtcgttaaa gtctttgaat ataccaatta
     3121 aattattaac ttcgttctca aggaacttaa atggaaaatc aggaaattat catccagggc
     3181 attacattgg ccggcaagcc tttccgcccc agcgactggg tggaccgtat gtgcagcacc
     3241 tacgccacct ttggcgatga caagaaattg cgttactcac cttacctgaa accaaagctg
     3301 gtgaataacg ttcggagctt gtccgtagac atgaaattga aggatgtaaa cccggccggt
     3361 tttgagcaac tgatgcagtt tgccaaggaa aaccagctca gcgtgatcag ccctgccggg
     3421 gaagccgtca ccatcgagta agcggtcatt tagcctccgg cgcggtggct ttgagcaagg
     3481 actccaacca aactttgctc aaggcaccgc gctttatttt atagagcgag ccatctggcg
     3541 cataaatgta agtggtaggc agcacctcgg gagttgcggg cagatgcttt tgctggtcgc
     3601 cgaggatgat ggggtaggaa atcagcatat cttcaatata attccgcacc gattgctcag
     3661 ttttatattg cacagcgacc cccagcacca tgacttcttt ggcatgggcg tcataaaagg
     3721 tcacgagttc aggcatttcc tccagacaag gcgggcacca gggcgcccaa taattcacca
     3781 caacccatcg ccctttatac tgggcgagct ggtgagtccg accagcgagg tcctgcatca
     3841 gccaccttga atctgccgcc agagaagttt cagcaacacc caggctgaat accgccagca
     3901 aaatagcaaa caggccgaat gtccgttgcc accccttgtt tttattgaaa atacgcccca
     3961 tatgaatccc gtagacggaa aaaacttgat tatgctggaa aactgttgct atactagctt
     4021 tatctcgttt ggtaatttca actttcagca aattactaat tcaaacaaaa attagccaca
     4081 cattcagaat ttttctgttt aaatccaatc tacatttgat ttaaattctt caccattctt
     4141 acgaggcaat aatgagcgac aacattaccc atttaagcga cgccagcttt gaacaagaag
     4201 tattgcaatc cacgattccg gtcctggttg attactgggc tgaatggtgc ggtccttgta
     4261 aaatgattgc acctatcctg gacgacatca gcaaagaata tgctggccgc ctgaaggtcg
     4321 ccaagctaaa tattgatgac aaccagaata caccacctaa atacggtatc cgtggcattc
     4381 ctacgctgat gttgtttaaa aatggcaatg ttgaagcgac caaagtaggc gcattgtcca
     4441 agtcccaact cagcgcgttt atcgatagca acatttaaca ctcaaccccg gtttccattc
     4501 tgcgaaaccg ggttttcttt ttaatctctc cgacgtttct cagtcttgcg agttactcat
     4561 gcacttatcc gatcttaaac atcttcccgt gaccgaactg gtcgaaatgg ccattgccaa
     4621 tgatattgaa aatgctagcc gcatgcgcaa gcaggacctt atttttgcca tcctcaaaaa
     4681 caaggcaaaa aaaggtgata gcatttttgg tgacggcaca ctggaagtct tgccggacgg
     4741 atttggcttt cttcgctccc cggatacctc ttacctggct ggcccggatg atatttacgt
     4801 ctcgccttcg caaatacgtc gcttcaacct gcatacaggc gatagcattc agggtgaaat
     4861 ccgcacaccc aaggatggcg agcgctattt tgcgctagtt aaagtcgaca gcgtcaatgg
     4921 tgaagcgcct gagaacacca aacacaaaat actgttcgag aacctaacac cactgttccc
     4981 gacggtaccg ttaaccctgg aacgtgaaat ccgcggtgaa gaaaacatca ccagccgcgt
     5041 catcgatatg attgcaccga ttggcagagg ccaacgtgcg ctactggtcg ccagccccaa
     5101 aagcggtaaa accgtcatgc tgcaaaatat tgcgcacgcc attaccgcta accatcctga
     5161 ctgtgtcctg attgtattgc tgattgatga acgtccagaa gaagtgacag aaatgacacg
     5221 ctcggtacgc ggcgaagtgg tcgcctcgac ttttgacgaa ccagcaacgc gtcatgtaca
     5281 agtcgctgaa atggtgctgg aaaaagccaa acgcctggtt gaacataaaa aagacgttgt
     5341 gattttgctt gattccatta caaggctggc gcgtgcttac aacacggtca tcccatcttc
     5401 cggcaaggta ttgaccggcg gcgtggacgc gaatgccctg caaaaaccca aacgtttttt
     5461 tggtgctgca cgtaacgtgg aagaaggcgg ctcactgacc attattgcaa ctgcattggt
     5521 cgataccggc tcacgcatgg atgacgtgat ttacgaagaa tttaaaggca ccggcaacat
     5581 ggaaatccat ctggatagac gcatggccga aaaacggatc tacccagcta ttaatgtcaa
     5641 caaatccggc acccgtcgtg aagaactgtt gctggacaaa gatgtgctgc aaaaaatctg
     5701 ggtcttgcgc aaactgcttt acccgatgga cgacctcgaa gcgatggagt tcttgcttga
     5761 taaaatcaaa ggcaccaaga gcaataacga cttctttgac agcatgcgcc gcggttaacc
     5821 ggccgatgga tacaattatt tatcgctctt ctattgataa attcatcaaa atcatagata
     5881 atcgcgcgtt tactgcaact gcttaagatt agagagaaaa gcatgaaagc tgatattcac
     5941 ccaaattacc cggaaatcaa cgtcacatgt agttgtggca acaaattcaa aacccgttct
     6001 actaacggta aagacctgaa cattgaagta tgttcacaat gccatccgtt ctacactggc
     6061 aaacagaaga tcgtggatac cgcaggtcgc gttgagaaat tccgccaaaa atacggcatg
     6121 taatttcggt caatacggtt caaaaaggca gcatggctgc cttttttgtt tttcactgtt
     6181 ggcacttcga ggttaatata ttagccatca agtgcgttct tttgcgaccc gaattcaaac
     6241 atgcagtttt atattgatca tgactggcaa gaaaatatgg ccaccccgcg agccaaagtg
     6301 ggggagcgcg ccaaaacgca tctgctgata ttactttgcg cgatttggct ctgcgtcggc
     6361 ctcatcggcc actcaccttg gaagcccttt gagtcacagt ccgccagcat catccagaac
     6421 ttgatcgacc acccttttct ttcttccagt agctggattc atggcgactg gattgcaccc
     6481 acatccgccg gcaacccgac gctggaaacg cctccattat tctactggac ggcaactggc
     6541 tttgcccact tgttgtcacc actgctccct acacacgatg cagctcggct aagtgtgggt
     6601 ctctggatgc ttctgacgct gattatgacc ggactcagcg gtcgcgagtt atggaatttg
     6661 ggcgtcggtc gtcaaaccaa ttttattttt atcggctgcc tagggctcgt ggttagtgct
     6721 cacaccatga tgccagccat cgccgcactc agcggcatca ctatggcatt ttacgcctta
     6781 gcactcatta tgcgtaaacc tgtgcgggcc atgtggttac tctgtacggg cttgttagtg
     6841 gcttttttgt ctggtggtgt cgtaccggct ttaaccatta tattatcagt cacctgcctg
     6901 ttaaccctac ccgcgattcg tcaacagaaa cacgtttacc gtgccatgat cgccggcaca
     6961 ttgtttgcat tgccgggcat ctatttatgg tgctggcttt gccagcaatg ggcgccagga
     7021 ctatggcaat cctggtggca agcacaatgg caattccagc tgccaggtaa tcatggttat
     7081 ttcttgagaa cactcgcatg gtatgcatgg ccagctttac cattcgcctt atgggggatc
     7141 tggcgttata ggcactccgt actcaatact taccgcttcc agttaggact gatattcttt
     7201 ggtatcgctt ttatcctgat cggcctctta agtgaccgta gcgaagtcag cgccttacct
     7261 ttactggttc cgctgacggt attagcggcg ggcagtatag aaacactcaa acgcggagcc
     7321 gctggcgcct tgaactggtt tggcctgatc ttgtttggta tcctgatatc tatcgcctgg
     7381 ctgggttggt gggccatgtt aaacgggact cccgtcaaac tgtaccaacg cctgagctat
     7441 ttatccggtt tggaaacgat tccattcagt ttactgtacg gtgcgattgc tgtcgccgtg
     7501 accctgatct ggttatctat tgtcttgcgt tccaagcact ccaaccgctc cagcgccacc
     7561 aactgggcca tcggcatgac ttgtgcctgg acagtattca tgagcttgtg gttacccatg
     7621 atagaggcag ctcgtaccta ccaaccgctg ttctcagatt tacgtaagca cttgcctgca
     7681 cattacaact gcattcacgc acaaaacatt ggcacatcac aggctgacct gttgcactat
     7741 catgccggcg ttaaattgga atatcacgca ccgggtgcca ccacgcagtg tgatttgtac
     7801 ctgattgaag atcaaccagg caaacgtcac gcgttaccgg gtgagcgctg gcacgttatc
     7861 tgggaaggtg aacaaacccg tcaaaataaa gagtctttca ggttactgca acgtcgttaa
     7921 ggctgcttgt atgaactttt agtgagactt tcaacggtat agaagaaata tcagtggtgg
     7981 tgacgatatt cgatgtacgt tcaaagatgt acatgctgtt gaatcgccgc aatcaccttg
     8041 ggatgcaatc cctgcccctg atgttgctgc acatgggcca cgagcgtttt aatcatgatt
     8101 gaattccaga atttcttcag atggctggca atctcccgct tggcttcctc aacatcagga
     8161 ttcgcctcaa aaaaatcgcc aatctggttg gccattttaa ttaacttatc aacttccata
     8221 tccttattct tcctatcagc tttcatgctc acgatgagca gctaactctc cgctatacac
     8281 gacacactgc gtagttttag cgaacccaac cagcaaaaaa tcatatttct gcgccaaact
     8341 gactgccaat gccgtggggg ccgataacgc aaccagcgac ctaccgccgc agacagcgac
     8401 tttctgtacc atttcataac tggcacgact ggtcgtcaac acccagtgcc cggaccaaaa
     8461 gcgtgaaaag tcttggcctt gacgcaatag tgcgccgatc agtttatcca gcgcattgtg
     8521 gcggccaaca tcctctcgca cagagaccac ttgcccttgc gcatcgacga tagcgctcgc
     8581 atgggttgcc ccggtcagtt gctgtaacgg ctgcttcaac ctgagcgcat ccaatgcagc
     8641 ctctatcgca cacaatgaaa gtgacaccaa cggctctccg ccaaccatgg gttgcttgaa
     8701 tacttgttgc agactctcag ccccacacaa gccacacccc gtacggccgg tcaaattgcg
     8761 ccgctgcgcc ttgagcgcga caaactgttg tgttgccaat tcaatgtgta attctatgcc
     8821 ctgtagttga ccatccacct gcatcaatgc cttggcttca acaccataga tttggctact
     8881 ctctgtaata atgccttcag taaatgagaa accataagca aaatcttcga gatccgctgg
     8941 ggtcgccagc atcactacat gtgaaatacc gttatacacc agcgcaacgg gcacttcttg
     9001 cgccaaggca tctgtgacga cagtggactg ttgttcacgc caccgtgtca ccgtcagagt
     9061 gctcactgcg cgctcagcag gaatcacttg ccattcgcta ctggcaggca acgcgcaatc
     9121 ctcaccttct actacttcaa tcagcatcaa gagactaccg tttgcggatc catcccagat
     9181 aactcaatct gtgtatcact aaatgcacgg taatgctgtt gccagtctga tagttggttt
     9241 accctggata cttgcacggc cgtcacttta tattcagggc aattggtcgc ccagtcggag
     9301 ttatccgtgg tgatcacatt ggcaccggat tcaggatgat ggaaagtggt atacacgacg
     9361 ccgggctgca cgcgctcggt aatcttggcc cgtaacacgg tttgaccggc ccggcttgtg
     9421 atcccaaccc aatcgccttc tgcaatcccg cggtcttcag catcgtgcgg atgaatttca
     9481 atcacatctt catgatgcca ggctacgttg tgcgtacgac gcgtttgtgc accaacgttg
     9541 tattgggaca agatacggcc agtggttaga atcaatgggt atttctggtt tactcgttca
     9601 gttgtcggca catactgtgt aataaagaac ttgcccttac ctcgcacaaa ttcatccaca
     9661 tgcatggtcg gtgtaccttg tggcgcctca tcattgcatg gccattgaat actgccaagc
     9721 tcatccagtt ttttgaagct gactccggta aaggttggcg tcaacgcagc aatttcgtcc
     9781 atgatttcgc tggcatgggc ataatgcatg ggataaccca gcgcctcaga gaatttagcg
     9841 gtgatttccc agtcttccat gccgttcttg ggcgtcatca ccttacgcac aggtgaaatg
     9901 cggcgctcgg cattggtgaa cgtaccattc ttctctagga aagaggcacc tgggaagaat
     9961 acatgggcaa atttagccgt ttcattcagg aacaaatcct gcactatcac acactccata
    10021 ctttccagcg cgtgcgtcac gtgttgcgta tctggatcag actgggcaat atcttcaccc
    10081 acacaataga cagccttaaa gctaccttcc gcagccaggt ccagcatatt gggaatacgc
    10141 aaaccagggt cagcgctcaa tgtgacgccc caagcctgct caaactgggc acgggtttca
    10201 tcatcggaca catggcggta gcctgggaac tcatgcggca tcgaccccat atcacaagag
    10261 ccttgcacgt tattctgacc acgcaatgga tttacaccta cgccttcacg gccaatattg
    10321 ccggttgcca tggccaggtt ggcaatcccc atgactgtgg tagaaccctg gctatgctcg
    10381 gtcacaccca aaccatagta aatggccgca ttaccgccag tggcatataa acgcgcagct
    10441 tcacgcacca cattagctga aacacccaac tctttttcca acgcctccgg ggagttttca
    10501 ggcttggctg caaaatcacg ccaggcaaca taggaatccc attcacaacg tgctttgaca
    10561 aagccttcct gcaccagacc ttcagtcaca atcacatgcg aaatcgcaga aatcatggcc
    10621 acattggtcc caggacgcag tttaagatga taatcggcac gaacatgcgg ggagttgtcc
    10681 accaaatcaa tttctcgcgg atcaaccacg atcagtttgg caccttcccg caagcgacgc
    10741 ttcatgagcg agccaaatac aggatgacca tcggtaggat ttgcccccat gacaaaaatc
    10801 acatctgatt tcatcacaga gtcaaacgtt tgtgtacctg cagactcgcc cagggtttgc
    10861 ttcaggccat aaccggtagg actatggcaa acgcgggcac aagtatccac gttattgacg
    10921 ccgaatacgg cacggatcaa tttctgggtg acatacacct cttcattggt gcaacggctg
    10981 gaggtgatac cccccacagc ctccttgcca tactgtgcct ggatgcgctt gatttcgctg
    11041 gctgcataat taatggcttc ctcccagcca atcttctgcc aggggtcatg aatgctttta
    11101 cgtatcatgg gggtggtaat acggtcggcg tgtgtggcat agccccaggc aaaacgccct
    11161 ttgacacaac tatggccgtg attggcaccg ccttctttgc taggcgtcat gcgtacgacc
    11221 tgttccccct tcatttccgc atcaaaactg caacccacac cacaataagc acaggtggtc
    11281 gtcactttat gctcaggggt gcctgcttca atcacggttt tttccataag cgttgcagtg
    11341 gggcacgcct gtacgcaagc cccacaggag acacattccg aatcaagaaa atccttgttg
    11401 cctgcagata ccttgctctc aaaaccacga ccctgaatcg tcagggcaaa tgtcccctgc
    11461 gtttcctcac aggctcgaac gcagcgtgaa cagacaatac acttggaagg atcaaacgtg
    11521 aaataggggt tggattcgtc tttggactga cccagatggt tctcaccctc atatccataa
    11581 cgcacttcac gcaggccaac cgcaccggcc atatcttgca gttcgcaatc gccattagcg
    11641 ccgcaggtca gacaatccag tggatggtca gaaatataga gttccatgac gccacggcgg
    11701 atgtcggcta acttaggtgt ttgtgttttg accttgagtc ccggggccac aggcgtcgta
    11761 cacgaagccg gataaccacg gcgcccttca atttcaacca ggcataaccg gcaagaaccg
    11821 aagggctcta atgaatccgt tgcacacagt tttggcacgg tcactccgcc caattgagcc
    11881 gcatgcatga tagaagtgcc atctggcacg gtcacatcca caccgtcaat attcagtgtc
    11941 accatggtcg cactatcaga tttcggcgta ccgtaatcag tgttagggtc atacttcagc
    12001 actggtggct gtgaagagga gtatttgatg tcatccataa tgtcaccctt tgctgccatc
    12061 aggcagcagc ttctttatta gtcagaccaa agtcttcagg aaaatgattc aaggcactca
    12121 gcaccggata aggcgtcatg ccgcccagtg cgcacaatga accattcagc atggtgtcag
    12181 acaaatcccg caacaactgt acattttttt gatgctgttt gcccgacaca atcttatcca
    12241 tgacttcaac accgcgtgta gagcctatgc ggcaaggcgt acacttaccg caactttctt
    12301 cagcacaaaa ctcgaaggca tagcgcgcca tcttggccat atcgacactg tcatcaaaag
    12361 cgacaatccc gccatgccct aacacagccc aaatcttggt aaattcttca tagtcgagcg
    12421 gtgtatcgaa ttggctttcc ggcaagtaag cgcccagtgg accgcctacc tgcactgccc
    12481 ggataggacg ccccgaagcg gaaccaccgc cgtagtcata cagcaattca cgcaaggtcg
    12541 cgccaaaagc caactcaacc agaccggttt gttttaaatt accagccagt tggataggca
    12601 aggtgccacg tgaacggccc atgccgtaat cggcataata ctgcgcaccc ttatccagaa
    12661 tgataggcac ggtcgccagc gaaatcacat tattgacgat agtcggctga ccaaataacc
    12721 cttcaatggc aggtagcggc ggcttgaaac gtaccagtcc gcgtttacct tcaaggcttt
    12781 cgagcaatga agtttcttca ccgcagacat aagcgcctgc tgcacgacgc acttccaagt
    12841 ggaaagtgtg actggaacca agaatatttt cacctaaata tcctgccaga ttggctttgc
    12901 ggatggcttc attcagcgtt tttaaggcat gcggatattc actacgcaaa tagatatagc
    12961 cttgtgttgc gccaaccgca atcccggcaa tcgtcatccc ttcaatgagg acaaaaggat
    13021 catcttccat gaccatacgg tcagaatagg taccggaatc tccttcgtct gcattgcaaa
    13081 caacatattt ctgctctgca ggagcattga gcacggtatt ccatttaatg ccggtcggga
    13141 acgcagcacc tccacgacca cgcaacccgg aatcagtaac cgccttaaca atttccgcac
    13201 ccgacatgga aagggcattt ttcaggcctt tgtagccatc atgcgctaaa taatcctcga
    13261 gtgatagcgg gtcaacaata cctacccttg caaatgtcag gcgttgctgc tttttcagcc
    13321 aggccagctc atcggtcaag cctaaataca aagcatgtgc tttagctttt tctgccaagg
    13381 cattggtaaa ttctgcatcg aacaaaccag gcacgtcgcc gggctgcact gggccaaaag
    13441 caacacggcc ttgtgcagtt tcaacctcca ccagtggttc tagccaaaac aaaccacgtg
    13501 acccgttacg aaccaactca acctgcactc cacgcaattg cgcttcctga gcgattttct
    13561 ttgcagtgcg ttcagcacct aaagaaagcg ctgtcgaatc agtggggaca tacaccttaa
    13621 ccatggcaca cctctgcaat caaatcggca actttgtcag cattcatgcg accatgcact
    13681 tcatcatcca gcatgatggc aggcgaacaa ccacaattac ccaggcagta gacggggagc
    13741 agcgtcacat taccgtcggc agtcgtctga tggtaatcca cgcccagttt ggctttcagg
    13801 gctgcttcca gcttttcgga gcccatagac tgacatgact cggcacggca tacctgcaat
    13861 acatgcttgc caatggggtg cgtacggaaa tgatgataaa aagagacgac gccatgcact
    13921 tcggccacag acaaggccag cgctttggca atttcgggat acacagtttc cggtacgtag
    13981 ccaatatcat cctgaatcgc atgtaacaaa ggc
//
LOCUS       DATMSK010000239         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_301450,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000239 DATMSK010000000
VERSION     DATMSK010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301450"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="VNZ84_07940"
     CDS             complement(<1..1445)
                     /locus_tag="VNZ84_07940"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWU66925.1"
                     /translation="MKLAIAQMNCMVGAMQQNAERILSLANEAYQAGARLLLTPELAL
                     CGYPPEDLLFRQDFHTANQRALQWLEQQLPAEMAVIVGHPHDVAGKHYNAASVIHSGR
                     IQATYHKHHLPNYSVFDEERYFEAGDLPVVFEWHGVRYGILICADSWEPGPALQALQA
                     NAQVLLTINASPYHIHKQQTRYQVLQARIKETSLPMVYANMVGGQDELVFDGASFVMN
                     SQGELMCELPMFEEHLAYIEIEDTQPLPAQITTPLSTEAAVYKALRLGLQDYVNKNKF
                     PGVVLGLSGGIDSALTLAIAVDALGKERVKAVMMPSEFTAGISVEDAAEMARLVEVEY
                     SVLPIKPLFEDFCDTLATEFAGKPFDTTEENLQARIRGMLLMAISNKFGSLVITTGNK
                     SETAVGYSTLYGDMAGGFALIKDVPKTLVYRLASYRNLISPVIPERIITRAPSAELRA
                     NQTDQDSLPAYDILDAIMEAYVERDLSVDAI"
     gene            complement(1468..2523)
                     /locus_tag="VNZ84_07945"
     CDS             complement(1468..2523)
                     /locus_tag="VNZ84_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWU66926.1"
                     /translation="MTHIPQTTSYRLSLLLCGGILLLGLSLHANAASKNKSKPTPDSP
                     IIDKSAMEVPPLDTSADISYLLIASSKGDIATVKALLKSGANPNVTDGEKITPIMYAA
                     RKNQAEVIRELLAHGADINAKDQGGWTALMFAAKKNNIDAVKVLLEKGADVKVRDPAG
                     WSALGLAATSGFSEVVGLVIARGFDANVRASDGKTVLMYAAKSGDVPTITAVLTHGAD
                     PNLKDKLGTTALMIAAREGHTPAVTYLVEHGAEVDEEDEHEWTALTWAVQKNQAEVAK
                     TLLDHKADVNHKDSEGTPLLHLAVSNNSPEMAQLLLKYEANVKTRDQYGLTAYVYALK
                     GQNPAMVELIKAAGGKY"
     gene            complement(2543..3424)
                     /gene="sucD"
                     /locus_tag="VNZ84_07950"
     CDS             complement(2543..3424)
                     /gene="sucD"
                     /locus_tag="VNZ84_07950"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWU66927.1"
                     /translation="MAILVDKNTRVLCQGFTGKQGTFHSEQALAYGTKMVGGVTPGKG
                     GQQHLDLPVFNTMREAVKETQANASVIYVPPPFAADSILEAADAGIELIVCITEGIPA
                     LDMLNVKAALRANGTKLIGPNCPGVITPDECKIGIMPGFIHKRGKVGVVSRSGTLTYE
                     AVHQTTALGLGQTTCVGIGGDPIKGLNFIECLELFQKDPETEAIIMVGEIGGNDEEAA
                     ADFIKHHVTKPVAAYIAGLTAPKGKRMGHAGAIISGGSGKAEDKITALEAAGCVVADS
                     PAGLGEAVLAAMQRAKK"
     gene            complement(3427..4548)
                     /gene="sucC"
                     /locus_tag="VNZ84_07955"
     CDS             complement(3427..4548)
                     /gene="sucC"
                     /locus_tag="VNZ84_07955"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWU66928.1"
                     /translation="MATPTGLFVQSEEAAAAATKELGGEAWVVKAQVHAGGRGKAGGV
                     KVVKSALEAQTVAGGLLGKHLVTYQNAPDGQPVHQVLVEQTLPIARELYLSMLVDRSL
                     ERVVMVASSAGGMDIEEIAATQPEKILQEICDPLNGLVDYQARNLAFGLGLNGDQVGA
                     FTKLAKGLYKLFKDNDLSLLEINPLIVTTEGKLVALDCKMIVDDNALYRQKPFAEIRD
                     WSQDDAKEAEAHHSGLNYIALNGNIGCMVNGAGLAMATMDLIKLHGGMPANFLDVGGG
                     ATAETVTKAFKIILSDQNVKAILVNIFGGIMRCDIIATGIITAVKEVGMTIPVVVRLE
                     GTNVELGKQMLRDSGLNIISAEGLTDAAQQAVTAVSKGV"
BASE COUNT         1004 a         1212 c         1148 g         1185 t
ORIGIN      
        1 atgatggcgt caacgctaag gtcacgctca acgtaagctt ccataatcgc gtccaggatg
       61 tcatatgccg gcaggctgtc ctggtctgtc tggttggcac gtagttcagc actgggtgcc
      121 cgtgtgataa ttctttcagg aatcaccggg ctgatgaggt tgcgatagct agctaggcgg
      181 taaaccagtg ttttaggcac atccttaatc agcgcaaaac cgccagccat gtcgccatac
      241 aaggtactgt aacccacagc cgtttctgat ttgttgccag tggtgatgac caggctgcca
      301 aatttgttgc taattgccat caataacatg ccgcggatac gtgcttgcag gttttcttca
      361 gtggtatcaa agggcttgcc agcaaattct gtcgccaggg tgtcacaaaa atcttcgaag
      421 agcggtttga ttggcaatac gctgtactca acctccacca gacgcgccat ttcggcggca
      481 tcctctacgc tgataccggc ggtaaattct gaaggcatca tgactgcttt tacgcgctct
      541 ttgcctaagg catcgactgc aatagccaat gtcagcgcgg aatctattcc accagacagg
      601 cccaatacta cgcctgggaa tttgttttta ttgacataat cctgcaagcc cagtcgcagc
      661 gctttgtata ctgcagcttc ggtggatagt ggtgtggtta tttgagcggg taaaggctgt
      721 gtatcctcta tctcaatata ggctaaatgc tcctcgaaca taggcagttc gcacatcagt
      781 tcaccctggc tgttcatgac gaatgaggca ccatcaaata ccagttcatc ttggcccccg
      841 accatattgg catataccat gggcaagctg gtttctttga tacgcgcttg cagtacctgg
      901 taacgcgtct gctgtttatg aatgtggtag ggcgaagcat tgatggtcag cagtacttgc
      961 gcgttagctt gcagtgcttg caaggcgggt cctggctccc aactgtcggc acaaatcaaa
     1021 atgccatagc gtacgccatg ccattcaaag accacaggca gatcgcccgc ttcgaaatag
     1081 cgctcttcat caaacacgct atagttcggt aggtgatgtt tgtggtaagt ggcctggatc
     1141 ctgcccgagt ggatcacgga tgccgcattg taatgtttgc ctgcgacatc atgcggatgg
     1201 ccgacaatca cagccatctc tgctggcaac tgttgctcca gccactgcaa tgcccgttgg
     1261 ttagcggtat gaaaatcttg cctgaacagc aagtcttcgg gaggataacc acacaaggcg
     1321 agttctgggg taagcagcag gcgggcgcct gcctggtaag cctcattggc tagtgacaga
     1381 atacgttccg cattctgctg catggcaccg accatacagt tcatttgagc gattgcgagt
     1441 ttcatggtgc ctatcgggac tgtggtatca atacttgcca ccagccgctt taatcaactc
     1501 caccatcgcg gggttttggc ctttaagtgc atagacataa gcggttaggc catattgatc
     1561 gcgggtcttg acgttggcct catacttaag cagtaattgc gccatttctg gtgagttatt
     1621 gctgacagcc aggtgcagca acggtgtgcc ttcactgtct ttatgattca catccgcttt
     1681 gtggtctagc aatgttttag cgacttcggc ctggttcttc tgaacggccc aggtcaaggc
     1741 tgtccattca tgctcatctt cttcgtctac ttctgcgcca tgctctacca aataagtcac
     1801 ggcaggagtg tggccctccc tggcggcgat catcaatgcg gtcgtgccta gtttatcctt
     1861 gagattgggg tctgcgccat gtgtcagtac ggcagtgatg gtcggcacat cgccactttt
     1921 tgcagcatac atgagcactg ttttaccatc tgaagcccgc acattggcgt caaagccgcg
     1981 cgcaatgact aaaccgacca cctcactaaa cccggaagtt gcagccaggc caagtgcgct
     2041 ccagccagcc ggatcgcgca ctttgacgtc tgcacccttt tcgagcaata ccttgaccgc
     2101 atcaatatta tttttcttgg cggcaaacat gagtgcggtc cagccgcctt gatccttggc
     2161 attgatgtca gcgccatgcg ccagcaactc acgtatgacc tctgcctggt ttttgcgcgc
     2221 ggcatacatg atgggtgtga ttttttcacc atcggtcaca ttagggtttg caccgctttt
     2281 gagcaaggct tttaccgttg caatgtcacc cttggaggag gcaatcagca gataggaaat
     2341 atctgcactg gtatccaaag gaggcacctc cattgcagac ttgtcaataa tggggctgtc
     2401 aggggttggt ttgctcttgt ttttgctggc tgcattagca tgcagggata accccagcaa
     2461 caggatgcca ccgcacagca gcaggctgag acggtagctt gttgtctgag ggatatgcgt
     2521 catggtttct cctaaaaggg gattattttt tggctcgttg catcgctgcc aagacagctt
     2581 cgccaagacc ggccggtgag tcggcaacaa cacagccagc cgcttccagt gcggtaatct
     2641 tgtcttcagc tttgcccgat ccgcccgaaa taatggcccc ggcatgaccc atacgcttac
     2701 cttttggtgc cgtcaggccg gcgatataag ctgccactgg tttggtgacg tggtgcttga
     2761 taaagtcagc ggcagcctct tcatcattac ccccaatttc gccgaccata atgatggctt
     2821 ctgtttcagg gtctttctga aagagttcca ggcactcgat aaagttcaag cctttgatcg
     2881 ggtcgccacc aatgccgaca caggtcgttt ggcctaaacc tagggcagtg gtttgatgca
     2941 cggcttcata agttagggtg ccggagcgag aaaccacacc gaccttgcca cgtttgtgga
     3001 taaagccggg catgatgcca atcttgcatt catcgggtgt aatcacgccc gggcaattcg
     3061 ggccgatcag cttggtgcca ttggcgcgca aggctgcttt gacattaagc atatccagtg
     3121 ccggaatacc ttctgtaatg cagacaatta gttctatgcc ggcatccgcc gcttcaagga
     3181 ttgaatctgc ggcaaatggt ggcggcacgt aaatcacgct ggcgttggcc tgggtttctt
     3241 tgaccgcttc acgcatggta ttaaatactg gcaaatccag atgttgttga ccacctttgc
     3301 ccggtgttac gccaccaacc atttttgtgc cgtaggccag cgcctgttca gagtgaaatg
     3361 taccttgttt accggtaaag ccctggcaca acactctggt atttttatca actaaaatcg
     3421 ccatgactac acacctttac tcactgctgt taccgcttgc tgggcagcat cggtcaaccc
     3481 ttcagcagag ataatattca aaccactgtc gcgcaacatt tgcttgccca gctccacgtt
     3541 ggtgccttcc aggcggacaa cgactgggat ggtcatgccg acttccttca ctgcagtaat
     3601 aatccctgtc gcaatgatgt cgcagcgcat gatgccacca aagatattga ccaggatggc
     3661 tttgacattc tggtcagaca aaataatttt gaaagccttg gtgacggttt ctgcagtcgc
     3721 accaccgccg acatccagaa agttggctgg catgccgccg tgcagtttga tcaggtccat
     3781 ggtcgccata gccaggcctg cgccattcac catacagccg atattgccgt ttaatgcaat
     3841 atagttaagc ccactgtgat gcgcttcggc ttccttggca tcgtcctgtg accagtcacg
     3901 gatctcggca aatggtttct gacggtagag cgcattgtcg tcaacgatca ttttgcaatc
     3961 cagtgcgaca agtttgcctt ctgttgtgac aatcagcggg ttaatttcta gcaaggacaa
     4021 gtcattgtct ttaaacagtt tgtagagccc tttggccagt ttggtaaatg cgcctacctg
     4081 gtcgccgttt aaacccaggc caaaagccag gttgcgtgcc tggtaatcca ccaggccgtt
     4141 gagtgggtca cagatttcct gcaggatttt ttctggctga gttgctgcaa tttcttcaat
     4201 atccatgcca cctgcgctag aggctaccat cacgacgcgt tccaatgaac ggtctaccag
     4261 catggataaa tatagttcac gcgcaatcgg caatgtttgc tccaccaata cctggtgtac
     4321 tggttggcca tcaggcgcat tctgataagt gaccaagtgc ttgcctagca agccaccggc
     4381 aaccgtttgt gcctcaaggg cggatttcac caccttgaca ccaccggctt tgccacggcc
     4441 acccgcatgg acttgcgcct tgacaaccca ggcttcaccc cctaattctt tggtggcggc
     4501 ggctgcagct tcttcagatt ggacgaacag accggtcggg gttgccaaa
//
LOCUS       DATMSK010000240         5077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_230080,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000240 DATMSK010000000
VERSION     DATMSK010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230080"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VNZ84_07960"
     CDS             complement(<1..426)
                     /locus_tag="VNZ84_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HWU66929.1"
                     /translation="MQSQRFWFQATKSILRVGITVAIAIGAGLLAYKLWVRYTDYPWT
                     RDGRVRADVVNIAPDVAGLVTAVPVHDNQYVHKGDVLFRIDTMHYQHALAEATALTQQ
                     RKSLWEMKKQQSARRAHLDDEVISRENREDTDLGAMAAKG"
     gene            complement(438..650)
                     /locus_tag="VNZ84_07965"
     CDS             complement(438..650)
                     /locus_tag="VNZ84_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HWU66930.1"
                     /translation="MMHEWIPREFAFLDAHVPTLFVAFLLAAAAIWLMDKALAAWGIY
                     DLVWYPALLRVALFFGLFSVFGLMVY"
     gene            complement(647..2827)
                     /locus_tag="VNZ84_07970"
     CDS             complement(647..2827)
                     /locus_tag="VNZ84_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HWU66931.1"
                     /translation="MQPINRGMQRLLAGLSTLGQGVRFVADCLQQAATDWYRQDFPLI
                     GYLFKYLLAALLALWVALKMEMDQPATALLTVVIVMHFRSGMVITKSYYRLIGTVIGI
                     LFSMLLVAWFAQDRFPFFIAAALWIGMCTAGSLVYRNFQSYGFVLAGYTLCIVGLPAT
                     LTPWHTFQIASTRMSEIGVGLLSATLVSELVLPQRLWENIQAVVRSKFRDFSVLLGTG
                     APAGLNQPFLKFMQDVYQLEAFRSSAQFETDDARVHSATINRLNVQFMAVSTTYVVLQ
                     RLIQRLHSRAALKEADALQALMQPLSAAVLIDGQPASHAEAITQVQQAVAAFQQDFDA
                     CLAVQLQHLMMMHPEVNQLEVETGGELLSRLAKELAAYLETYALLVAQPRARKVASRE
                     FTADQLHMHIDWLPVSIAAIRGALTLLILAAIWMTIDWPSGILAMTLGVITSTLFAAS
                     PAPMATIRQFSVGVLLGIVLVYISNVFWLTAAHDYVMLCIVITPAILLTAWLSARPAT
                     ALVGAGLGIAYFLMVGFNQALGDNPVKYFNDSIALMVALVVSGIMFSLTDYAASPWAQ
                     ARVFTQLRKLVVDACLHTGMTAPGFEMRTRDLIQRSGSVHRPQAAESVRVVEALCAAL
                     EVGHAVLALRAVARGLSAQPYQLVQRTLTLVARYYKQPGLVQQQAVLQWLDHFLAWLQ
                     GGEYADALLSSQARKLITQLHFIRLVIAAELPQDLASTSTGAAS"
     gene            complement(2814..4280)
                     /locus_tag="VNZ84_07975"
     CDS             complement(2814..4280)
                     /locus_tag="VNZ84_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWU66932.1"
                     /translation="MPLTIIAILPHQPLRVSRWGVWLLALLLNGCISSKGIIPASQPL
                     AQDKLALGKAIEDASAIAWPTEHWWQAYQDTQLDRLVEKSLRDHPDLKKATARIALTT
                     AMAEQAHAATLPNVGGKVSSSRERFTELSFIPKPWAGRFNWNNQATVDMSYNLDLWKQ
                     QKSAWLAALDETHVSEAEMQQVKLELTAAMVKSYIRLAAEYQWRDLAADELDDLRHEV
                     AIAKKALAAGLNTQLSLSRLEAQLPAAQNKLAQIELHLTLLKNQIAALSGEGPGAGES
                     IARPSLHLSASIGLPDQLPANLVGRRPDIVAARWRVEASQKHIQSAKAAFYPNINLLG
                     YIGFQALAFSGLFSSAGLVGNLGPAMSLPIFDGGKRRANLSAETALYDMAVEDYNATI
                     LKSLESISNQLSILNTVAVEAGNTQQALEKSEQAHRLAEKNYRAGLSNRVESLQTNAL
                     VLQQKSILVEQEAVRLEAYAALMLALGGGVIDAEHATH"
     gene            complement(4348..4851)
                     /locus_tag="VNZ84_07980"
     CDS             complement(4348..4851)
                     /locus_tag="VNZ84_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU66933.1"
                     /translation="MLLLKELPTSKSLEKFVTRYPGTDISAISDFVSILRAASDISSA
                     LDALLAQHGLLQGRWWVLVLLMRQDDLTSSPSELAEKAGVTKATMTGFIDGLEREGLV
                     SRFMDTNDRRKFLIRLSVAGQQKLDEVMPVYVQCVTQFMNVLGRPERNTLITDLATLA
                     SRVDLIK"
     gene            complement(4952..>5077)
                     /locus_tag="VNZ84_07985"
     CDS             complement(4952..>5077)
                     /locus_tag="VNZ84_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWU66934.1"
                     /translation="KHNIVSDVEMIDANYINDAYERMLKSDVKYRFVIDIASMRS"
BASE COUNT         1296 a         1475 c         1231 g         1075 t
ORIGIN      
        1 acctttggcg gccatggcac ccagatcggt atcttcacgg ttttcacgtg aaatgacttc
       61 gtcatctaga tgtgcacggc gggcagattg ctgtttcttc atttcccaca gggactttct
      121 ttgctgggtc aatgcggtcg cctccgccaa tgcatgctga taatgcatgg tgtctatgcg
      181 gaacaacaca tcccctttgt gcacatactg gttatcatgc acaggcacgg ccgtgaccag
      241 cccggccaca tccggtgcaa tattcaccac atcggcacgt acacggccat ctcgtgtcca
      301 tgggtagtcg gtatagcgca cccacaactt ataggctaac agtcccgcgc cgatcgcgat
      361 cgccaccgtg atgcctacac gtaaaatgct ttttgttgcc tgaaaccaga aacgctgtga
      421 ctgcatagac gagcctctca atacaccatc aaaccaaaca cggaaaacaa accaaaaaac
      481 aaggcaaccc ttaacaaggc tggataccat accaggtcgt aaattcccca ggcggccaat
      541 gctttatcca tcagccagat cgcggcagcg gcgagcaaaa acgccacaaa caaagtgggc
      601 acatgggcat ccagaaaagc aaactcccgc ggaatccatt catgcatcat gatgcagctc
      661 cggttgacgt tgaagccagg tcctgcggca gctctgccgc gataaccagc ctgataaaat
      721 gcaattgcgt gatgagctta cgcgcttgtg aagacaggag tgcatcggca tactccccac
      781 cttgcaacca ggccagaaaa tgatccaacc attgcaacac ggcttgctgc tgcaccaacc
      841 ctggctgctt gtaatatctg gcaaccaacg tcagtgtgcg ctgcactaat tgataaggct
      901 gtgccgatag ccccctggca accgcacgca acgccaggac cgcatgccct acctctagcg
      961 cagcacacaa ggcctcgacg acacgcacac tttcagccgc ttgtggacga tgcacactgc
     1021 cggagcgctg gatcaaatcg cgtgtgcgca tttcaaaacc gggcgctgtc atgcctgtat
     1081 gcagacaagc atcaaccact aacttgcgca attgggtaaa cacgcgggcc tgcgcccagg
     1141 gactcgccgc ataatccgtc aggctgaaca tgatgccgga caccaccaat gccaccataa
     1201 gcgcgataga gtcattaaaa tacttgaccg ggttatcgcc cagcgcctgg ttgaatccca
     1261 ccatcaaaaa gtaggcaatg cccaagccag cccccaccaa cgcagtggcg ggccgtgcac
     1321 tgagccaggc tgtgagcaat atggcaggcg taatcacgat acagagcatg acataatcat
     1381 gtgccgcggt gagccagaat acattgctga tgtacaccag cacgatgccc agcaacaccc
     1441 ctactgagaa ctggcgaata gtggccatcg gcgccggact tgccgcaaac agggtgctgg
     1501 taatcacccc cagcgtcatg gccaaaatgc ctgagggcca gtctatggtc atccagatgg
     1561 ccgccaggat cagcaaggtg agtgcgccgc ggattgcagc aatcgacacg ggcaaccagt
     1621 caatgtgcat atgcaactgg tcagcggtga attcgcggct cgcgactttg cgcgcacgtg
     1681 gctgagccac taacaaagca taagtctcca ggtaggcagc caactctttt gccagccgcg
     1741 acagcaactc gccgccggtt tccacttcca gctgattgac ctccggatgc atcatcatca
     1801 aatgttgtaa ctgcaccgcc aggcacgcat caaaatcttg ctgaaacgct gcgaccgctt
     1861 gctgcacctg cgtaatggct tcagcatgac tggccggctg cccatcaatc agcaccgcag
     1921 cagataaggg ctgcatcaag gcctgcaaag catctgcttc ttttaacgcc gcgcgggagt
     1981 gtaaccgctg gatcagccgc tgcaagacga cataagtcgt agaaactgcc ataaactgca
     2041 cattgagccg gttgatggtt gcactgtgta cccgcgcatc atccgtttca aactgtgcag
     2101 acgaacgaaa tgcctccaac tgatacacat cttgcataaa ctttaaaaac ggctggttga
     2161 gccctgcagg tgcgcccgtg cccagcaaga cgctaaaatc acgaaacttg ctgcgtacga
     2221 ctgcctgaat gttttcccac aggcgttgtg gcagcaccag ctcgctgacc aaggtcgcac
     2281 tgagtaaacc gacgccaatt tcagacatac gggttgaggc gatttgaaag gtatgccatg
     2341 gcgtcagcgt cgcaggcaag ccgacaatac acaacgtata tccagccaag acaaacccgt
     2401 aagactggaa gttacgatat accagcgaac cggccgtaca catgcctatc cagagcgctg
     2461 ccgcaataaa aaacgggaag cggtcttgcg caaaccaggc caccagcagc atggaaaaca
     2521 aaatcccgat caccgtaccg atcagacgat aataactttt ggtgatcacc atgccggaac
     2581 ggaaatgcat cacaatcacc accgtcagca aggcggtcgc cggttgatcc atctccattt
     2641 tcaaggccac ccacaacgcc agcaaagccg ccagcaagta tttgaacaaa tagccaatca
     2701 agggaaaatc ctgacgatac cagtcagtag cggcctgttg cagacagtcg gccacgaatc
     2761 gcacgccttg accaagcgta gacaagcctg ccagcaaccg ttgcataccg cgattaatgg
     2821 gttgcatgtt cagcatcgat cacccctccg cccagcgcca gcatcaatgc cgcataagct
     2881 tccagccgca ccgcctcctg ctccaccaga atgctctttt gttgcaatac cagagcgttg
     2941 gtttgcaggc tttccacccg gttacttaag ccggcacggt aatttttctc cgccagccga
     3001 tgcgcctgct cgcttttttc caacgcctgc tgcgtattcc cggcttcaac cgcgaccgta
     3061 ttcaggatag agagctggtt ggaaatactt tccagcgact tgagaatagt cgcgttgtaa
     3121 tcctctaccg ccatgtcata cagcgctgtt tctgccgata ggttagcgcg gcgcttacca
     3181 ccatcaaaaa taggcaatga catggcaggg cccaggttgc cgaccaaacc agcactggaa
     3241 aacaggccgg aaaaggccag tgcctgaaag ccgatatagc ctaacaaatt gatattcgga
     3301 taaaacgcag ccttggcact ttgaatatgt ttttgcgacg cttcgacccg ccatctggca
     3361 gcgacaatat ccgggcggcg gcctaccagg ttggcaggta actgatctgg caggccgatg
     3421 ctggccgaca aatgcaaact aggccgggcg atgctctcgc ctgcaccagg cccttcgcca
     3481 ctgagtgccg caatctggtt tttgagcaaa gtgaggtgta actcaatctg cgccagctta
     3541 ttttgtgccg caggcaattg cgcctccagg cggctcaggc ttaattgcgt atttagcccg
     3601 gcggccagcg cttttttagc aatggcaact tcatggcgca ggtcatccag ctcgtctgct
     3661 gcgaggtcgc gccactgata ctctgccgcc aggcggatat agcttttcac catggctgcc
     3721 gtcagctcaa gcttaacttg ctgcatttcg gcttcactga catgggtttc atccagcgcc
     3781 gccaaccagg ctgatttctg ttgcttccat aagtccaggt tgtaagacat gtctaccgtc
     3841 gcctggttgt tccagttaaa gcggcccgcc cacggcttgg ggataaaact caactcggta
     3901 aagcgttcgc gtgacgagct cactttacca ccaacattcg gcaaggtcgc cgcatgcgcc
     3961 tgttcggcca tcgccgtcgt caaggcaata cgcgccgtgg cttttttgag gtccgggtga
     4021 tcgcgcaggc ttttttcgac caggcggtct aactgcgtat cctgatacgc ctgccaccaa
     4081 tgctcagtcg gccaggcaat ggcggaggca tcctctatcg ccttgcccag ggccagttta
     4141 tcttgcgcca gcggctgcga cgcggggatg atacctttgg aactgatgca tccatttaat
     4201 agcagggcca ataaccagac gccccagcga gacactctca ggggttggtg cggcaatatg
     4261 gcaatgatag tcaatggcat aaatataaag tatggctatt gatagtatgg aatcaaacta
     4321 aataacaaaa aaatttactc gatcaattta cttgattaaa tcgacccgac tggccaaagt
     4381 ggcgagatcg gtaatcaagg tatttctttc cggcctaccc agcacattca taaactgcgt
     4441 gacacactgc acatacaccg gcatcacttc atccagtttt tgctgcccgg ccaccgataa
     4501 gcggataaga aatttacgcc tgtcattggt atccataaaa cgtgacacca gcccttcacg
     4561 ttccaggcca tcaataaagc cagtcatggt cgccttggtc acgccggctt tttctgccag
     4621 ctcggaagga gatgaagtca ggtcgtcctg gcgcatcagc aacactagca cccaccagcg
     4681 cccttgcaac agcccatgct gagccagcaa tgcatccagt gcagaagaaa tatccgaggc
     4741 tgcgcgcaaa atgctgacga aatcagaaat ggctgaaatg tctgtgccgg gataacgcgt
     4801 cacaaacttt tccaagcttt tggaggtagg taattcttta agtagcagca tagtacggac
     4861 cagtatagta tggcatcaaa ctaaatgcaa gtgctccgta atcaaaaaag ccctgtgttc
     4921 taaacagggc tttggtacac catcgtcgcg tttaagacct catggatgca atatcaatca
     4981 caaaacgata tttcacatca ctcttgagca tacgttcgta agcgtcatta atgtagttgg
     5041 catcgatcat ttctacatcg ctgacaatat tatgctt
//
LOCUS       DATMSK010000241         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_441763,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000241 DATMSK010000000
VERSION     DATMSK010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441763"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="glp"
                     /locus_tag="VNZ84_07990"
     CDS             complement(<1..1067)
                     /gene="glp"
                     /locus_tag="VNZ84_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWU66935.1"
                     /translation="MNNQDLLQQLASNPSCADDYDPNAMSVPQARQYIQQFLSPVQET
                     ETIEIKQALGRILAAPITSTINVPGHNNSAMDGFAFRHEEATQALKLAGTAFAGNAFT
                     GSLPAGGCIKIMTGAVIPTGADTVVMQEQTSTQGDMVTLSKVPALGANIRLIGEDIAF
                     GQTVLSRGHRIQPADMGLLASLGIAEVQVYRRLKVAFFSTGDELISVGQPLQSGQIYD
                     SNRYSLWGMLQALGVDVYDLGNVADDPEALEKTLLQAASHHDVVITSGGVSVGEADFM
                     KQLLAKHGQVLFWKINMKPGRPLAYGKIGQSHYFGLPGNPVSTMVTFYQFVQEALQCL
                     MGSSAINTPSFKVECVTAIKKA"
     gene            complement(1070..1639)
                     /gene="mobA"
                     /locus_tag="VNZ84_07995"
     CDS             complement(1070..1639)
                     /gene="mobA"
                     /locus_tag="VNZ84_07995"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HWU66936.1"
                     /translation="MQITAVILAGGRGMRMGGADKGLVDYQGKPMIEHVLTRITPQVS
                     QVMINANRNLDRYRHYGVPVITDNNDQFDGPLAGMQAGMQHAKTDWIVSVPCDSPLLP
                     LDLAQRLSQAIQTIQAISHPPAMLAIARSSSGIHPVFCLMPRTLGDDLNAYLESGQRR
                     VSAWQARHPHVFVDFADEQAFTNINQPST"
     gene            complement(1639..2649)
                     /gene="moaA"
                     /locus_tag="VNZ84_08000"
     CDS             complement(1639..2649)
                     /gene="moaA"
                     /locus_tag="VNZ84_08000"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWU66937.1"
                     /translation="MQTTTAIPSQLIDQFGRRVDYIRLSITDRCDFRCQYCMAEDMTF
                     LPREQVLSLEECARLVKLFVRMGVRKVRITGGEPLVRKNALWLFEEIGHLPGLDECVL
                     TTNGSQLEKYATALKSAGVKRINISIDSLQEDRFKNITRVGELPKVLSGLDAAISAGF
                     DNIKINSVLMHGMNGDEAEALVNFSIQKQIDISFIEEMPLGEVNHNRAQTYVSNTDTL
                     TLLKSKFSLTPSTHRTGGPARYWQVNGHTTKIGFISPHSHNFCESCNRVRITCQGELF
                     LCLGQDHQVNLMPLLRAYPDDDQPLVDAILHGMHIKPQGHDFDLRRAKPSVVRFMSHT
                     GG"
     gene            2758..3258
                     /locus_tag="VNZ84_08005"
     CDS             2758..3258
                     /locus_tag="VNZ84_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HWU66938.1"
                     /translation="MVGCEVGLDNTLSNEQHAQIQAHIQAHEHVPGGLMPLLHAIQDD
                     IGYVPEVVYPEIAKALALSVAEVHGVVTFYHHFRTHPVGKHVLQVCRAESCQSMGSEK
                     LEAELKAKLGVDYHQTTADGSVTLLPVYCLGNCGCSPAVMLDDEVYGRMNTEKVTELI
                     AEVCHG"
     gene            3251..>3567
                     /locus_tag="VNZ84_08010"
     CDS             3251..>3567
                     /locus_tag="VNZ84_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HWU66939.1"
                     /translation="MVKVYVPIDSTALSLGAERTAKKIIQEAQARGVDITLVRNGSRG
                     LFWLEPLVEIETAQGRVAFGPVQPSDVLSLFEAGFTDALSDSARAHPLYLGLADELAW
                     LKK"
BASE COUNT          843 a          858 c          940 g          926 t
ORIGIN      
        1 gcttttttga tagcggtgac gcattcgacc ttgaaggagg gcgtgtttat agcgcttgat
       61 cccatcaagc attgcagtgc ttcctgtaca aactggtaaa acgtgaccat ggtcgatacc
      121 ggattgcccg gcaagccaaa ataatgcgat tggccgattt tgccgtaggc taacggacgg
      181 cctggtttca tgttgatttt ccagaacagg acttggccat gtttggccaa gagctgtttc
      241 ataaagtctg cttcgccgac ggatacgccg ccactggtaa tgacgacatc gtggtgactg
      301 gctgcctgca atagcgtctt ctctaaagcc tctggatcat cggccacatt gcccaaatca
      361 tatacatcga cacccagcgc ttgtaacatg ccccacaggc tgtagcggtt gctatcatag
      421 atttgtcctg attgcagcgg ttggccgacg ctaatcagct catcacccgt gctgaagaaa
      481 gcgactttta atcgacggta aacctgcact tctgcaatgc cgagcgaagc cagcaaaccc
      541 atgtcagctg gttgtatccg gtgaccgcgc gacagaacgg tttgcccaaa agcgatatct
      601 tctccgatta accggatatt ggcacccaaa gccgggactt tgctgagtgt taccatgtca
      661 ccttgggtag aggtctgttc ctgcatgact acggtgtcag cccccgtcgg gatcactgcc
      721 ccggtcatga ttttgatgca gccgccagcc ggcagtgagc cggtgaatgc attgcctgca
      781 aacgctgtac ccgctaactt tagtgcttgc gtcgcttctt cgtgcctgaa ggcaaatcca
      841 tccatggcgg aattattgtg ccccgggacg ttgatggtgg aagtgatagg cgcagccagt
      901 atgcggccta acgcctgttt gatctctatc gtttcggtct cctgaacagg tgacagaaat
      961 tgctggatat actggcgtgc ttgtggcacc gacatggcat ttgggtcata gtcatcagca
     1021 caagaaggat tgctggcgag ttgctgtaaa agatcttggt tattcataat cacgttgaag
     1081 gctgattgat gttagtgaat gcttgctcat ctgcgaaatc gacaaataca tgaggatgcc
     1141 tggcttgcca tgcactgaca cgacgctgtc cgctttctaa gtaggcattg aggtcatcac
     1201 ccagtgtgcg gggcatgagg caaaaaacag gatgtatgcc actgctactg cgggcaatcg
     1261 ctagcatggc tggtgggtgg ctgattgcct gtatggtttg aatggcctgg ctgagtcgct
     1321 gcgccaggtc taatggcaat aagggggagt cacaggggac gctgactatc cagtccgttt
     1381 tggcatgctg catgcccgct tgcatgcccg ccagagggcc atcgaactgg tcattgttat
     1441 ccgtgatgac gggtacgcca taatgacgat agcggtccag attacggttg gcattgatca
     1501 tgacttggct cacttgtggc gtgatgcgcg taagcacatg ctcaatcatg ggtttacctt
     1561 gataatccac cagccctttg tcagccccgc ccatgcgcat gccgcggcca ccggccagaa
     1621 tcactgcagt aatctgcatt aaccacctgt gtgcgacata aagcgcacta ccgatggttt
     1681 tgcgcggcgc aaatcgaaat catggccttg cggtttaatg tgcatgccat gcaaaatcgc
     1741 atctactaag ggttgatcgt catcaggata agcgcgcagc aagggcatta agttaacctg
     1801 gtgatcttgc cccaaacaca ggaacaactc cccttggcag gtgatgcgca cccggttaca
     1861 gctttcgcag aaattatggc tgtgtgggga tataaagcca attttggtgg tatgaccgtt
     1921 cacttgccag taacgggctg gcccaccagt tctatgggtg ctaggggtga gtgagaactt
     1981 actttttagc agtgtcagag tgtctgtatt actgacgtag gtttgcgcgc ggttatgatt
     2041 gacttcacct aatggcattt cttcaataaa gctaatgtcg atctgttttt gaatcgaaaa
     2101 attcaccaaa gcttctgctt catcaccatt catgccatgc atcagtacgc tattaatttt
     2161 gatattgtca aacccagctg aaatcgctgc atccagcccg ctcagtactt tgggcagctc
     2221 accgacacga gtaatgtttt taaaacggtc ttcctgtagg ctgtcgatgg aaatgttaat
     2281 gcgtttgacc cccgccgatt tgagtgcggt agcatatttc tctagctggc taccattggt
     2341 agtgagcacg cactcatcca aacccggtaa atgcccaatt tcttcaaaca gccagagggc
     2401 gttttttctg accagtggtt caccgcccgt aatgcgaact ttacgcacgc ccatacggac
     2461 aaacaattta acaaggcgcg cacattcttc gagtgacaag acttgctcac gtggcagaaa
     2521 agtcatgtct tcggccatgc aatattggca acggaaatca caacgatccg tgatcgacag
     2581 gcggatatag tccacgcgac gaccaaactg gtctatcagt tgtgaaggaa tggcggttgt
     2641 tgtttgcatg acagtattaa cgtctaaagt aattcgtatt ttatgccctt tttatgcttt
     2701 cacaaggcat aaatagagga tatgattcga ggatgataga atgtaactga attgtaaatg
     2761 gttggatgtg aggtcggttt ggataacaca ttaagcaatg agcaacacgc gcaaattcaa
     2821 gcgcatattc aagcacacga gcatgtgcca ggcggattaa tgcctctgct acatgcgatt
     2881 caggacgata ttggttatgt ccctgaagtt gtgtatcccg agattgccaa agcgctggcc
     2941 ctgtctgtgg ccgaggtgca tggtgtcgtc actttttatc atcatttccg tacccaccct
     3001 gttggcaaac atgtcttgca ggtttgccgt gccgaatcat gccagtccat gggttccgaa
     3061 aaactcgaag cagaactcaa agcgaaattg ggcgtggatt accaccagac cactgctgac
     3121 ggtagtgtga cactgttgcc tgtgtattgt ctgggtaatt gtggctgttc accagccgtt
     3181 atgctggatg acgaagtcta tggtcgcatg aatacagaaa aagtgaccga gttgattgct
     3241 gaggtgtgtc atggttaagg tgtatgtgcc gattgactcg actgcactct cactgggtgc
     3301 cgagcggacg gccaaaaaaa tcattcagga agcacaggcc cgaggcgtag atatcacgct
     3361 tgtgcgcaat ggttcacgcg gtctgttctg gctggaaccg ttagtggaaa tcgagactgc
     3421 acaggggcgt gtggcatttg gcccggtgca accaagcgat gtattgagcc tgttcgaggc
     3481 aggctttacg gatgccttgt ccgattcagc cagggcacat cctttatatc tgggcttggc
     3541 cgatgagttg gcctggttga aaaaaca
//
LOCUS       DATMSK010000242        15135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_42825,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000242 DATMSK010000000
VERSION     DATMSK010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 15135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15135
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42825"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VNZ84_08015"
     CDS             <1..292
                     /locus_tag="VNZ84_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU66940.1"
                     /translation="SMRTLMSVMKMNKASIYAAYGSKESLFKKTVECYVNGSASFLMA
                     SLHQPTALAVVQTMLSQAAFMLADRSHPAGCLLIQGDSLCEAGHSEIKLLLK"
     gene            332..556
                     /locus_tag="VNZ84_08020"
     CDS             332..556
                     /locus_tag="VNZ84_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66941.1"
                     /translation="MRAIKEKDLLPAAEPTVLAKLVMTTHQGMVIQALSGATQTQLFN
                     VAVMATQMIAQAYTVKPGFCDDNAEMKATV"
     gene            complement(605..1159)
                     /locus_tag="VNZ84_08025"
     CDS             complement(605..1159)
                     /locus_tag="VNZ84_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU66942.1"
                     /translation="MNMRDKILNIASSLFDSRGIQASGVDTIILEAGVAKATLYKHFP
                     SKNQLITAYLRDKSDKFYAWLNAQMASKKVESQELLLLLCELMAQWISQPEFRGLPFH
                     IAAVEFPEAEHPINQYSVVLSAELQGYLCEIARHAGAKDPEALGQQLTILFEGAALVE
                     RLTPNTGAAARAKEAAMTLVRNAL"
     gene            complement(1218..1970)
                     /locus_tag="VNZ84_08030"
     CDS             complement(1218..1970)
                     /locus_tag="VNZ84_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66943.1"
                     /translation="MNHTRQRYERICKAYAAHGLWAIGLKKWAKHAPLYRLRNNLAGW
                     NRQRIQQAGAASVLNVQDISSIRQLIDQAQQQSTLPLSPALLDQWFFLAIGAVQVESQ
                     TASSKAWQLFDQSIHSQLEKPAFYRSLSFGLIVSLCVMWMAFAPVKQNQIQAELSPLE
                     SSVSQARGSADPVTLSLLNLAYQKMQNGSCQLPQAAMLPEVQRQAFIMFVTEGKVDTD
                     QVEPLRQALGYVSCLYPQELMRPQGRDRLPRE"
     gene            2173..3219
                     /locus_tag="VNZ84_08035"
     CDS             2173..3219
                     /locus_tag="VNZ84_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWU66944.1"
                     /translation="MTSVSIATQLAEWRDHALALENEVKKVVVGQDKAIRAINIAIFA
                     RGHVLLEGGVGVGKTTLLQSIARGLGGAYERIEGTVDLMPNDLIYHTFLGEDGRPKVD
                     EGPLLRAGEQLSVFFFNEINRARPQVHALMLRVMAERHVHAFKKEYRLPHMLVFADRN
                     QVEKNETFEIPSAARDRFMMEIPIEIPDDRNLRKALMFDVKFQHAERLTQQVRSGVLA
                     YDQLNGFSDVLQSHIRASDVLEDYALDLWQATQSPAAFGIQLDSVDVNRLIHTGASPR
                     GMGMLLKAARVHAWLMERDSLYPEDIHAVFHEIIAHRLVLNPMFESRRTELSRELTNQ
                     ILATVSVPSKAKAA"
     gene            3216..4076
                     /locus_tag="VNZ84_08040"
     CDS             3216..4076
                     /locus_tag="VNZ84_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWU66945.1"
                     /translation="MIPANAQPFFYQVPWRSASVHAGEHLGIQRGPGDEFSKHVSLVD
                     YPDARRLDLRQTLRDPYEQVQVRVFKQEASTPLYAVCDMSSSMQFQGQASKLDTLQAV
                     AASIACSAHENSDLFGMVSYDHAVLPHLSQAPGHHAHQSFATIAQLAEFHNARKDGQG
                     IVEATGWLGQQKGLVFWISDFHMPLALIEQGLNGMSNHQVIPVVLWDPQEYQRLPRFG
                     FGNLQDPETGQQRTIFFRQAIRAQFAEQFEQRKQALDQLFARFDIRPLYIAGHFEASL
                     LSDYFEQLIH"
     gene            4106..5017
                     /locus_tag="VNZ84_08045"
     CDS             4106..5017
                     /locus_tag="VNZ84_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66946.1"
                     /translation="MKQIFLGWLAVCLIAMPACAETQDTKVQPAEIKVLSVQNPATYA
                     GIQLGDVLQRTIRLSATAQDQLKENDLPLKGLQRNGIELRQLHVSSDMQSGKKLYTLL
                     FEYQVFASSGNPVQLQLPAERITFNSGAMVELPAWRFWLMAQLPDRLQPAKPTVIAQY
                     HPNMQPTDMVQRWLGVSLLLALTGSLILLYRNANWRWLPMMNGHFAQAYRQLKRLPAN
                     AEGQKQASLIMQKAFNARFGQQMLASHVPAFVAQQPELQTLESQIQAFFVQANAVLYG
                     GQQDGAVEYLKQCMALTRQLRDCERRV"
     gene            5017..5985
                     /locus_tag="VNZ84_08050"
     CDS             5017..5985
                     /locus_tag="VNZ84_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HWU66947.1"
                     /translation="MHWLHPWFLLLLPVAAVPFLLKSRHAQAYSWLEMVPEDTFSERI
                     HLLVKLATALLLLCMVLALASPQGDSHEEQKVGKGAQSVFVIDRSVSMDHPFAGQATG
                     GHAAEIKSAAARRLITAFIDSRPDDMMGVVGFTNSALYGMKITTNRDGIHAAIQAATG
                     PALNQTNIGAGITNAATLFENIPSTGSRAIVLLSDGAGKLSPRVKYQIRKQLVGKKLN
                     LYWIVLREPDDISIFTGNEYDDDHLPEAIALDRFFQSLNIKYKAFEADNATALESALR
                     EIDAKEKNVIQYGVIIPGHDYAKDLIVAALLLGLGLIFIKQIKVYS"
     gene            5982..6518
                     /locus_tag="VNZ84_08055"
     CDS             5982..6518
                     /locus_tag="VNZ84_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66948.1"
                     /translation="MTVYRKHANWLWGSIAVLGIVGALYFGWQLHMLRELNQQIRTGQ
                     VIKKGAEAYLQKLTAAYDQGQHGDYKHAVQTYSQALELAPPAAELPHIHFNIGNNLFL
                     SGLVRTINEDGTFQDEARYAFTQAKISYEQALRLQPEDKAAKFNLALLHSVMARGMQA
                     ASKDQSHMELSNLPIGLP"
     gene            6518..7474
                     /locus_tag="VNZ84_08060"
     CDS             6518..7474
                     /locus_tag="VNZ84_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HWU66949.1"
                     /translation="MKWMTWLKQHYEPVCYGLALVLLLLALVKPEIQLRQQVQHYLLI
                     ADISQSMNAEDETLNQKAVSRLAYTQHLMKQLVHKSPCGTYFSLGIFASDNIAMLQTP
                     LEVCKNLDVISDGIDHLEWRMAWKGNSRLSFAVHAATHVYDALNQPAQMLFFTDGDEA
                     PRVNVTIKQDLSGVQIGEFFTFIGVGGPQKVAIPRYNSANKAVGYWPVSDNNNAGAVG
                     VTYADPSQDEPDPPVAYAEYDRMMSQLEEDYLKSLADEFKGSYLKGRDQEAFYQTLAA
                     KPRTASFVTDYPLRSIYLVTAIMLILATYLPDIRFRRLKENA"
     gene            complement(7492..8649)
                     /locus_tag="VNZ84_08065"
     CDS             complement(7492..8649)
                     /locus_tag="VNZ84_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWU66950.1"
                     /translation="MPPLRFLIITTTLVGLLHYYIGSRLIDTLAWPAWVHWYGWGILV
                     ISALLQPLAFLARGFSNHALRDATSWVAMTMMGIFSSILVLTVLRDVLLLAAAGLHAA
                     HVINIDFAVLEKNSAMIAVAGALIITVIGFFNARKLARVVNVTVPLRDLPDSLHGFRI
                     VQISDIHVGPTIKQGYLSRIVQQVNSLEADLVAITGDLVDGRVHELAEHTRPLASLEA
                     RHGSFFVTGNHEYYSGALAWIHELERLGVRVLLNEHVQLTHEEATLVLAGVTDFTAGH
                     FYEDHHSDPQAAMTGAPADAPKILLAHQPRSAQAAQAAGFDLQLSGHTHGGQFWPWNY
                     FFLLQQPYVAGLHRLQDLWVYVSRGTGYWGPPIRFGAPSEITCLTLVPMHE"
     gene            complement(8760..9617)
                     /locus_tag="VNZ84_08070"
     CDS             complement(8760..9617)
                     /locus_tag="VNZ84_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HWU66951.1"
                     /translation="MSLRTLMIILLLLLALPSEAHAWGLYSHVAYTHQWLQALPLLPL
                     PWLSVMQRYPTLVMTGACLPDLAVVSRTFNHSHGWGMGQQLLQTKNERQLALGIGYNI
                     HLLTDVVAHQHFVPTFEAKWQHHSVLTHAAAEWAMDAYLHQPGMPSPGKLLRVHRKTI
                     VETLTHTLHCEAEIVHKAVSRLASADQALRLSHIPQYLLRRYRRLDNEFEHKLAYYRD
                     QVTLALQDLPQLLHGNFPSLHAEHINLGMEQLDDWRQKCLQDARLRPTRAIRAFEHYQ
                     HQWFNQPDC"
     gene            9749..10948
                     /locus_tag="VNZ84_08075"
     CDS             9749..10948
                     /locus_tag="VNZ84_08075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU66952.1"
                     /translation="MKILMISDVYFPRVNGVSTSIRTFAESLREQGHVVHLIAPQYPA
                     QEGQPLTIDESWITRIDARKIVFDPEDYLMKWSALMTYINSLHPGDYDLVHVHTPFIA
                     HYAGLRIGKRLQVPVVETYHTFFEDYMHHYLPWVPRKWAVRIAQTVSRKQCQQVDTVV
                     SPSAQMREVLQQYGVTTPITVIPTGLDASRFVAGNGAAFREKYGIPAQRPLLLFVGRV
                     AHEKNIQFLLEMLSLVIQDHPEVLLVITGEGPAEAMLKKMARDNGLQSHVMFLGYLDR
                     AVGLNAAYQAADIFVFASKSETQGLVLLEAMAQATPVVAIAELGTASILRQGQGALIA
                     QDDEVHFAEQVQTLLIDHQLRERVGKEGKRYVEQVWSSEAQANKLLECYERLMLAHMP
                     MAASAAA"
     gene            11070..11435
                     /locus_tag="VNZ84_08080"
     CDS             11070..11435
                     /locus_tag="VNZ84_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66953.1"
                     /translation="MFIPDSCCTLVPYFEVPAENLAVFKALGTQFLDKTRNEPDCMHY
                     AFSFSGNIAHCREGYANAEGILKHLENVAEPLGEALKLSKLIRLEVHAPAAEVEKLKA
                     PLAALNPQFFILEAGIRRQ"
     gene            complement(11507..12286)
                     /gene="pstB"
                     /locus_tag="VNZ84_08085"
     CDS             complement(11507..12286)
                     /gene="pstB"
                     /locus_tag="VNZ84_08085"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWU66954.1"
                     /translation="MANVTTAIKAEARDLNFFYSNGARALKGISMPLYENKITALIGP
                     SGCGKSTFLRCFNRMHDLYPGNKYQGQIVMHPDNTNVLDAGVDPIEVRMRISMVFQKP
                     NPFPKSIYENVAYGLRVRGENNKRIMDDKVEEALKGAALWNEVKDRLHDLAFNLSGGQ
                     QQRLCIARALATDPEIMLFDEPTSALDPIATANIEELMVELRSKLTILVVTHNMQQAA
                     RVSDYTAYMYLGELIEYDDTDRIFTNPSRKETEDYITGKFG"
     gene            complement(12312..13250)
                     /gene="pstA"
                     /locus_tag="VNZ84_08090"
     CDS             complement(12312..13250)
                     /gene="pstA"
                     /locus_tag="VNZ84_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWU66955.1"
                     /translation="MEHKASVLENLADVRAMIKRHKRNDTLFAAIGLLCLMLGLVTLM
                     LLFWQLIADGYPKFSLDFLTEFPSRRAGRAGLLSAWVGSLLVMSVTFLTAVPVGVAAG
                     LYLEEYAPKNWFTDLIEINVANLAGVPSIIYGLLALGLFVYMLDFGQSILTAGLTLGL
                     LILPIVIVSTREAIRAIPVSIREAAYGVGATKWQVVYDHVFKYSYGGILTGIIIGMAR
                     ALGETAPIITIGALTFIAFLPPSPITTEPPFINFEWLTSGFTVMPIQMFNWLSRPDHA
                     FHVNAAATGALIIIVTLLMNGAAIWLRYRIRKNIKW"
     gene            complement(13347..14273)
                     /gene="pstC"
                     /locus_tag="VNZ84_08095"
     CDS             complement(13347..14273)
                     /gene="pstC"
                     /locus_tag="VNZ84_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWU66956.1"
                     /translation="MQAPAQLTISPRLAKNIRRNVKERIIELILMLAALVAVATTFAI
                     VGVLLYESLSFFKTVSVVEFFTDTQWTPLFEDAHYGIMPLVSGTLTTSAIALAVAVPI
                     GTISAIFLSEFASHRTRETIKPILELLVGVPTVVFGYFALFFVTPLLQSIYPDLPSFN
                     MLGAGIVMGIMIIPYIASMSEDAMRAVPMSMREGSYAMGATRFQTAIRVVTPAAVSGI
                     VAAYILAISRAVGETMVVAIAAGQQPNLTFNPLDSAATITAYIVQVAMGDLPHGSIGY
                     QSIFAAGLVLMAMTLSFNIVGHWVRKKYRESY"
     gene            complement(14383..>15135)
                     /locus_tag="VNZ84_08100"
     CDS             complement(14383..>15135)
                     /locus_tag="VNZ84_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU66957.1"
                     /translation="DASRPISQKEIDACKEGGVQFIELPIAYDALTVVVNGKNDWIKQ
                     ITVAELKKIWEPGSKVKSWKEVNATYPDKPINLYGPGTASGTFDYFTEAVNGKAKASR
                     TDYTPSEDDNVLVQGVSGNVGGMAYFGLAYYLENTDKLKAVPVVAKEGGKAVLPSEAT
                     VMDGTYQPLSRPLFIYVNATSAAFRPEVKAFVNYYLENAPELVKEVKYVPLPKADYTA
                     VKEHFKAMKPGTGFNGSSEVGIKITDLLSRIK"
BASE COUNT         3714 a         3665 c         4009 g         3747 t
ORIGIN      
        1 ttccatgcgt acgttgatgt ctgtgatgaa aatgaataaa gccagtattt atgcggccta
       61 tggcagcaaa gagtctttat tcaagaaaac ggtagagtgt tatgtcaatg ggtcggcaag
      121 ctttctgatg gcttcccttc atcaaccgac ggctctggca gtcgtgcaga ccatgttgag
      181 ccaggcggcg tttatgctgg ctgaccggtc gcatcctgct ggatgcctat tgatccaggg
      241 cgactcgcta tgtgaggcag gacacagcga aattaagctg ttactaaaat gatatcgaga
      301 gacgtttgag caaaaaatcg cagagcgctt tgtgcgtgcg atcaaagaaa aggatttatt
      361 gccggctgct gagccaacgg tgctggccaa gctggtcatg acaacacacc agggaatggt
      421 tattcaagcg ttgagcggtg cgacacaaac gcaattgttt aatgtggcag tcatggccac
      481 gcaaatgata gcacaggcat acaccgtcaa gccagggttc tgtgatgaca atgcagaaat
      541 gaaggcgact gtttagagca aatgcagaaa ggttgccatc tttgtttaga tggcaacctg
      601 agtcttaaag tgcgttacgt accagcgtca ttgctgcctc tttggctcgt gcagcagcgc
      661 cagtattggg tgtcaggcgc tctaccaaag cggccccttc aaacaggatg gtcaactgct
      721 ggcccagggc ttccgggtct ttggctcccg cgtggcgtgc aatttcgcac aggtatccct
      781 gtaactcggc cgataggacc acagagtact gattgatcgg atgttccgct tccggaaatt
      841 caacagctgc gatatggaag ggtaggccgc ggaattccgg ctggctgatc cattgcgcca
      901 tcagttcaca tagcaagagc aacagctctt gtgattcgac ttttttcgag gccatctggg
      961 catttagcca ggcataaaac ttgtcagact tgtcacgcag gtaagccgta atcagctggt
     1021 ttttgctagg aaaatgttta tataaggtgg ctttggccac gcctgcttcc aaaataatgg
     1081 tgtctacccc tgaggcttgt atgcctcggc tgtcaaacag gctgctggct atattgagta
     1141 ttttgtctcg catgttcatc acttatcctt ttatcccgat tttcagggtt cccgatcttt
     1201 cgcttgttct cttttactca ttctctcggt aaacggtctc gaccttgagg acgcatcagt
     1261 tcttgtgggt acaagcaact gacataaccg agcgcttgcc gtaaaggttc cacctggtct
     1321 gtatctacct taccttctgt cacaaacatg atgaaggctt gacgctgtac ttctggcagc
     1381 atggctgcct gtggtagttg gcaactgcca ttctgcattt tctggtaagc caggttcagc
     1441 aggcttagtg tcactggatc agcgctccct ctggcctggc ttacgctact ttccaaaggc
     1501 gataactctg cttgtatctg gttttgcttg actggcgcaa atgccatcca catcacgcat
     1561 aaactcacta tcagaccaaa ggatagcgag cggtaaaacg caggtttctc caactggcta
     1621 tggatcgatt ggtcaaacaa ttgccaagcc ttgctgcttg ccgtttgaga ctctacctgc
     1681 accgcaccta tggccaggaa aaaccattgg tctagcaacg caggcgacaa tggcagcgtg
     1741 gactgttgtt gcgcctgatc tatgagctgg cgaatactcg aaatatcctg cacattgagt
     1801 acactcgctg cgccagcttg ctgtatgcgt tgcctgttcc agcccgccaa gttatttcgt
     1861 aagcggtaaa gcggggcatg ttttgcccat ttttttaatc caatggccca taagccatgt
     1921 gcggcatacg ctttgcatat gcgctcatac cgctgtcttg tgtgattcat gttcactaat
     1981 aaattcacgc caataacccc taaaaattca cttgcttttt tttcgatggc tgcattagac
     2041 tagaaaaaag tgaacagacc tgtctgcata ttatcgtgac atcacataaa atgcaaccag
     2101 tacgacgaac actcttttca atagttactt tggcatcata gctccggctg aatttgtaaa
     2161 caagggatta ccatgacatc tgtctccatc gcaacacaat tggcggaatg gcgcgatcat
     2221 gcgcttgcgc ttgagaatga agtaaaaaag gtcgtagttg gtcaggataa agcgatccgt
     2281 gccatcaata ttgctatttt tgcccgcggc catgtgttgc tggaaggtgg cgtgggtgtg
     2341 ggtaaaacca ctttgttgca atcgattgcc cgtggcctgg gtggcgctta tgagcgtatt
     2401 gaaggcacgg tcgacttaat gcctaacgac ctgatctacc atacattcct cggtgaagac
     2461 ggccgaccaa aagtggatga aggtccattg ttgcgtgcgg gcgagcagct ctcggtgttt
     2521 ttctttaacg agattaaccg tgcgaggcca caagtgcatg cactgatgct acgcgtgatg
     2581 gcagagcgtc atgtacatgc gtttaaaaaa gaataccgtt tgccgcacat gctggtgttt
     2641 gctgaccgca accaggtaga aaagaatgaa acctttgaaa tcccatcggc tgcacgagac
     2701 cgctttatga tggagattcc gattgagatt cctgatgaca ggaatttacg caaggcattg
     2761 atgtttgatg tgaagttcca gcatgcggaa cgactgacac agcaagtacg gagcggggtg
     2821 ctggcttatg accagctgaa tggtttctct gatgtgttgc aaagccatat tcgcgccagt
     2881 gatgtactgg aagattatgc actggattta tggcaagcca cgcaatcccc ggcggcattt
     2941 ggcatccagc tcgacagcgt ggatgttaac cgcctgattc ataccggtgc cagcccgcgc
     3001 gggatgggta tgctgctcaa agcggcgcgt gtacacgcct ggctcatgga gcgcgacagt
     3061 ttgtatcccg aggatattca tgcggtgttc cacgaaatta tcgcgcaccg cctggtcttg
     3121 aatccgatgt ttgaaagccg ccgcaccgag ttgtcgcgcg aactcaccaa ccagatactg
     3181 gcaactgtca gtgtgccatc caaggcaaaa gcagcatgat tccggccaac gcgcagccat
     3241 ttttttacca ggttccctgg cgctctgcca gtgtgcatgc aggtgaacac ttgggtatac
     3301 agcgtggtcc gggcgatgag ttcagtaaac atgtttcact ggttgattat cctgatgcca
     3361 ggcgcctgga cttgcggcag accttgcgcg atccctatga gcaggtacaa gtgcgtgtat
     3421 tcaagcaaga ggcttccaca ccgctgtatg cggtatgtga tatgtccagc tccatgcagt
     3481 ttcaggggca ggccagcaaa ctggatacct tacaagccgt ggctgcttcg attgcttgtt
     3541 cagcacatga aaacagcgat ttgtttggca tggtgagtta cgaccatgca gtgctcccgc
     3601 atctaagcca ggcaccgggc catcatgcgc atcaatcatt cgccaccatt gcccaattgg
     3661 ctgaatttca caatgcgcga aaagatgggc aggggattgt cgaggcaaca ggctggttgg
     3721 gccagcaaaa agggttggtg ttctggattt ctgattttca tatgccgttg gcgctgattg
     3781 aacagggcct gaatggcatg tccaatcatc aggtcatccc cgtggtgtta tgggatccgc
     3841 aggagtacca gcggttgcca cgctttggtt ttggcaattt gcaggatccg gaaaccggcc
     3901 aacaaagaac gattttcttc cgccaggcca tccgcgccca gttcgccgag cagtttgaac
     3961 agcgtaaaca agcactggat cagctgtttg cccgttttga tatccggcct ttgtatattg
     4021 ccggtcattt tgaggctagc ttgctgtcgg attattttga acaacttatt cactagcgtg
     4081 ttgtagtgaa accgaaagta tcgtgatgaa acagattttt ttagggtggc tggcggtgtg
     4141 cctgattgca atgccagcat gcgccgagac gcaggataca aaagtgcagc cggctgagat
     4201 caaagtattg agtgtacaaa acccggctac gtatgccggc atacagttgg gggatgtatt
     4261 gcaacgtaca atcagacttt cggccacagc gcaggaccag ctcaaagaaa atgacttgcc
     4321 gctaaaaggg ttgcagcgga atggtataga actcaggcaa ctacacgtca gtagtgacat
     4381 gcagtcgggc aaaaagcttt atacgctgtt gtttgaatac caggtatttg ccagcagcgg
     4441 caacccggtg caactgcaat tgccagccga gcgtattacg tttaacagcg gggcgatggt
     4501 tgagctcccg gcgtggcgtt tctggttgat ggcgcaacta ccggaccgct tgcagccagc
     4561 caaacccacc gtcattgcac aatatcatcc gaacatgcag ccaactgaca tggtgcaacg
     4621 ctggctgggc gtgagtctgc tgctggcgct gacgggtagc ttgatcttgc tctaccgtaa
     4681 tgcgaactgg cgctggttac ccatgatgaa tggccatttt gcgcaagcct atcgtcaact
     4741 gaaacgcttg cccgccaatg cggaggggca gaagcaggca tctttgatca tgcaaaaagc
     4801 attcaatgcg cgttttgggc aacaaatgct cgctagccat gtgccagcat ttgtggcaca
     4861 acaacctgag ctccagacgc tggaatcgca aatacaggcc ttttttgtgc aggcaaatgc
     4921 cgtactttat ggcggccaac aagacggggc agtggaatat ctaaaacaat gcatggcact
     4981 gacacgtcaa ttgcgtgatt gtgaacggag ggtgtgatgc attggttaca cccctggttt
     5041 ttgctgttac tccctgtagc ggcagtgcct ttcctgctga aaagccggca tgcccaagcc
     5101 tattcctggc tggagatggt gcctgaggat acgttttctg agcgcatcca cttattggtg
     5161 aagctggcga cagccttgtt gctgctgtgc atggttctcg cgctggcgag tccgcagggc
     5221 gatagtcatg aagaacagaa agtgggtaaa ggggcgcaaa gcgtgttcgt gattgaccgc
     5281 agcgtcagta tggaccatcc ttttgccggg caggccactg gcgggcatgc ggcggaaatc
     5341 aagtcggcag cagctagacg cctgattact gcatttattg actcgcggcc agatgacatg
     5401 atgggcgtgg ttggcttcac caactcggcc ttgtatggca tgaaaatcac caccaaccgg
     5461 gatggcattc atgccgctat tcaggcagca actggccctg cgctgaacca gaccaatatc
     5521 ggtgccggga ttaccaatgc tgcgaccttg tttgaaaata ttccaagcac cggatcgcgg
     5581 gcgattgtat tgctgtcgga tggggcgggt aaattgagcc cacgtgttaa ataccagatt
     5641 cgcaaacagc tggtgggtaa aaaactcaat ttgtactgga ttgtgctgcg tgagcctgac
     5701 gatatcagta tttttaccgg caacgagtat gacgatgacc acctgccgga agccatcgcg
     5761 ctggatcggt tttttcagtc acttaacatc aagtacaagg catttgaggc agataatgcg
     5821 acggcactgg agtctgcctt gcgcgaaatc gatgccaagg aaaagaatgt gatccagtat
     5881 ggcgtcatca ttcccgggca tgactatgcc aaagacctga tcgttgccgc tttgttgctg
     5941 gggttgggcc tgatattcat taaacagata aaggtgtact catgacagtt tatcgaaaac
     6001 atgccaattg gctttggggc agtattgcag tattaggcat tgtcggcgcc ctctattttg
     6061 gttggcaact gcatatgctg cgtgaattga atcagcagat tcgcactggt caagtcatca
     6121 agaagggcgc ggaggcctat ttgcagaagt tgactgctgc ttatgaccag ggccagcatg
     6181 gcgattacaa gcacgcggta caaacctaca gccaggcgct ggagttggcg ccaccggctg
     6241 cagagttgcc gcatatccat tttaatattg gtaataacct gtttttgtct ggactggtgc
     6301 gcacgattaa tgaagatggt actttccagg atgaagcacg ttacgccttt acccaggcca
     6361 agatttccta tgagcaagcc ttgcgtttgc agccggaaga caaggcagcc aaatttaacc
     6421 tggccttgct gcattcggtg atggctcgcg gtatgcaggc cgccagcaaa gaccagtcac
     6481 atatggaact cagtaatttg ccaataggat tgccataatg aagtggatga catggttaaa
     6541 gcaacactac gagccggtgt gctatgggct ggccttggtc ttgctgttat tggctttggt
     6601 taaacctgag attcagctta ggcagcaggt acagcattat ttactgattg cggatatttc
     6661 acaaagcatg aatgccgagg atgaaacact gaaccagaaa gcggtcagcc gtttggccta
     6721 tacccagcat ttaatgaagc agttggtgca taaatcgccg tgcggtactt acttcagcct
     6781 ggggattttt gcttcggaca atattgccat gctgcaaacg ccactggagg tgtgtaagaa
     6841 cctggatgtg atcagtgatg gtattgatca tcttgaatgg cgaatggcat ggaagggcaa
     6901 tagccggtta agttttgccg tacatgcagc gacccatgtc tatgatgcgc ttaaccagcc
     6961 ggcacagatg ctttttttta ccgatggcga tgaagcgcct cgcgtcaatg tcaccatcaa
     7021 gcaggatctg agcggggtgc agattggcga gttttttact tttattggcg ttggcggtcc
     7081 acaaaaggtg gcgattcctc gttataactc agcaaacaaa gctgtagggt attggccagt
     7141 cagcgataat aataatgccg gtgctgttgg ggtcacctat gccgacccga gccaggacga
     7201 acctgatccg cctgttgctt atgcagaata cgaccgtatg atgtcacagc tggaagagga
     7261 ctacttgaag tcgctggccg atgagtttaa aggcagctat ctcaaggggc gggatcagga
     7321 agcgttttat caaacgctgg cagctaagcc aaggacagcc agttttgtga cggactaccc
     7381 tttgcggtca atttacctgg tgacagctat catgttaatt ctggcgacct acctgccaga
     7441 tatccgcttc cgccgcttga aggaaaatgc ttagccctga gagtcacgag gctactcgtg
     7501 catgggaacc agggttaaac acgtaatttc agacggggcg ccaaagcgga taggtggccc
     7561 ccaataacct gtgccacgac tgacatatac ccacaagtct tgcaggcgat gcagcccggc
     7621 gacatagggc tgttgtagca ggaaaaagta attccatggc cagaattggc cgccatgggt
     7681 atggccggat agctgtagat caaatcctgc tgcctgagcg gcttgtgcac tgcgcggttg
     7741 atgggctaga aggattttag gtgcatccgc cggcgcgcct gtcattgctg cttgtgggtc
     7801 gctatggtga tcctcgtaaa agtgaccggc cgtaaagtca gttacgccgg ccaggactaa
     7861 ggtggcttct tcatgcgtga gttgcacatg ctcattgagc aatacgcgca cccccagccg
     7921 ttctagttca tgaatccagg ccaaagcgcc agaataatac tcatgattac ccgtgacaaa
     7981 aaagctgccg tggcgcgcct ccaggctggc taatggacgg gtatgctccg ccagttcatg
     8041 tacacggcca tcgaccaggt caccggtaat cgcaaccagg tccgcctcca gagaattgac
     8101 ctgctgtaca atgcgtgaca aatagccttg cttgatggtc gggccaacat ggatatcact
     8161 gatttgcaca atccggaagc catgcaggct gtcaggcaaa tcacgtagag gcacggttac
     8221 attgaccaca cgtgccagct tgcgcgcatt aaaaaagcca ataacggtga tgatcaacgc
     8281 gcctgccacc gcgatcatgg cgctgttctt ctccagtact gcaaagtcta tgttgatcac
     8341 atgcgcagca tgcaagcccg ctgcagcaag gagtaacaca tctcgcaaca ccgttaacac
     8401 caatatcgat gaaaagatgc ccatcattgt catggcaacc cagctcgttg catcgcgtaa
     8461 tgcatgattg ctaaatcccc gggccagaaa cgccaaaggt tgcaataagg ccgagatcac
     8521 caggattccc caaccatacc agtgtaccca ggcaggccag gcaagtgtgt ctatcaggcg
     8581 tgagccgatg taataatgca acaggccaac cagggtggtc gtgatgatga ggaaacgtag
     8641 agggggcatg tgtttttgag gggcagaaag atgtagatta gatggtgaca tccgctgaca
     8701 gattcaagtg tgccatgccg ataatatcta tatatccacg cagcgcatag gcgcgagaac
     8761 tagcaatcag gctggttaaa ccattgatgc tggtaatgct cgaacgccct gatcgcacga
     8821 gtggggcgca gcctggcatc ttgcaggcat ttctggcgcc agtcgtccag ttgttccatg
     8881 cctaaattaa tgtgttcagc atgtagcgac gggaagtttc catgcaatag ctgtggtaaa
     8941 tcttgtagcg ccaaggtcac ttgatcgcgg taatacgcca gtttatgttc aaactcgtta
     9001 tctagcctgc ggtagcgccg caacaaatat tgagggatat gcgacagccg cagcgcctga
     9061 tctgcactgg ccaatctgga taccgcttta tggacaatct cggcttcaca atgcagagta
     9121 tgcgtgagtg tttctacaat cgttttacgg tgcacacgca acagcttgcc aggtgaaggc
     9181 atccctggct gatgcagata tgcatccatc gcccactcgg cggcggcatg cgtgagtacg
     9241 ctgtgatgct gccatttggc ttcaaacgtg gggacaaaat gctggtgcgc tacaacatcc
     9301 gtcagcaagt gtatgttgta gcctataccc aaggccaatt ggcgctcatt tttggtctgt
     9361 aatagctgct ggcccatgcc ccaaccatga ctatgattaa acgtgcgtga cacaacggcc
     9421 aggtctggta agcaggcgcc agtcataacc agtgtcgggt agcgctgcat cacactgagc
     9481 cacggcaatg gcaagagcgg taacgcttgc agccattgat gggtatacgc aacatgtgaa
     9541 tataatcccc aggcatgtgc ttcactgggc agtgctagca gcaataatag tatgatcatc
     9601 agggtacgaa gtgacatgca cgcactttgc aattgttaaa tgacacttgg atgaagcctg
     9661 tatgacgttg atatgaaagc ggttttcgtc ataaaagcat catgaaagct tgttaagcta
     9721 tgtgagattt gttaactcgg aatcctgcat gaaaatattg atgatttcag atgtctactt
     9781 tcctcgtgtg aacggggttt ccaccagcat ccgtaccttt gctgaaagct tgcgtgagca
     9841 ggggcatgtt gtacatctga tcgcaccaca gtatccagcg caagaagggc agcctttaac
     9901 catcgatgaa agctggatca cccgtatcga tgcacgcaag attgtgttcg acccggaaga
     9961 ctatttaatg aaatggtcgg cattgatgac gtatatcaat agtttgcacc cgggcgatta
    10021 tgacctggta cacgtccata ccccttttat tgcccattat gccggactgc gcattggcaa
    10081 aagattgcag gtgccagtgg tcgaaaccta tcacaccttc tttgaagatt acatgcatca
    10141 ttacttgcca tgggtgccgc gcaaatgggc agtccgtatt gcacaaaccg tgtcccgtaa
    10201 acaatgccag caggtagata ccgtggtatc gccgtcagcc caaatgcgcg aggtcttgca
    10261 acaatatggc gtgaccacgc cgattacagt gatccccacc ggattggatg cctcacgatt
    10321 tgtggcagga aatggcgccg cgttcaggga gaaatacggg attccggcac agcgcccact
    10381 cttgttgttt gtgggtcgcg tggcgcatga aaagaatata cagtttctgc ttgaaatgtt
    10441 gtcactggtg atccaggatc atccagaggt gttgcttgtg attaccggcg aaggcccggc
    10501 tgaagccatg ctcaagaaaa tggcacgcga taacggcttg caatcacatg tgatgttcct
    10561 cggttacctg gatagggcgg ttgggctgaa tgcggcttac caggctgcag atatttttgt
    10621 gtttgcttcc aagagtgaga cacaagggct ggtcttgctt gaggccatgg cacaggcgac
    10681 gccggtggtg gcgattgccg aattgggcac tgcttccatc cttcgccaag ggcaaggcgc
    10741 actgatcgca caggatgatg aagtccattt tgccgagcaa gtgcaaacct tgttgattga
    10801 tcaccaatta cgcgagcgtg tgggtaaaga gggcaaacgt tatgtggaac aagtgtggtc
    10861 cagtgaagcc caggccaata aattgctcga atgttatgag cgtttaatgc tggcacatat
    10921 gcctatggcc gcctctgcag cagcctgaaa agggatgcct cattaaaatc ttgaattttc
    10981 accgggaggc ttgacgcctc cttcctgacc cgccaacatg atgccagact tgcactcatg
    11041 caagcaaacg ctattcagga gaatcaaaca tgtttattcc agacagctgc tgcacgctgg
    11101 ttccttattt tgaagtccct gctgaaaacc tggctgtttt taaggctctt ggcacccagt
    11161 ttttagataa aacccgcaat gagccggact gcatgcatta tgccttttcg ttcagcggga
    11221 acattgcgca ttgccgtgaa ggttacgcta acgcggaagg tattttaaag catctggaaa
    11281 atgtagctga gccactcggt gaggcattga agctgtccaa gttgatccgg ttggaagtgc
    11341 atgcccctgc ggcggaagtg gaaaagctca aggcaccatt ggcagcactg aacccacagt
    11401 tttttatact ggaagcgggc atacgccgtc agtaatcacg gcaagacact acaataaaaa
    11461 acgccacatg ctgtggcgtt tttcatgagt cacactcgga caggtattaa ccaaacttac
    11521 cggtaatgta atcctcggtt tccttgcggc taggattggt gaaaatacgg tcagtatcat
    11581 catattcaat caactcaccc aggtacatat aagccgtata gtcagacacg cgtgccgcct
    11641 gttgcatgtt gtgggtaacc accaggatgg tcagcttgct acgcagttct accatcagtt
    11701 cttcaatatt ggcggtggca attgggtcca gcgcggaggt cggttcgtca aacagcatga
    11761 tttctgggtc ggtggccagg gcacgtgcaa tacataaacg ttgttgctgg ccgccggaca
    11821 ggttgaatgc caggtcgtgc aggcggtctt tgacttcatt ccacaaggca gcccctttga
    11881 gggcttcctc taccttgtca tccataatgc gcttgttgtt ttcaccgcgt acgcgcaaac
    11941 cataagcgac gttctcgtag atggactttg gaaacggatt tggtttctgg aacaccatgc
    12001 tgatacgcat acgcacttca atcgggtcta cgccagcatc cagcacattg gtgttgtccg
    12061 ggtgcatcac aatctggccc tggtatttgt taccaggata taaatcgtgc atgcggttaa
    12121 agcaacgcag gaaggtggac tttccgcaac cggaagggcc gatcagtgcc gtgatcttgt
    12181 tttcgtacaa aggcatggag atccctttga gggcacgggc gccgttgctg tagaagaagt
    12241 ttaaatcgcg agcttcggct tttattgctg tagtgacgtt ggccatggag tttcctaaat
    12301 cttgtgtggc attaccattt aatattttta cgaattctgt agcgtaacca gatggctgca
    12361 ccgttcatta acaatgtgac aatgataatc agtgcaccag ttgccgccgc attgacatgg
    12421 aaggcatggt caggacggga taaccagtta aacatctgga tcggcatcac ggtgaagccg
    12481 gaagtcagcc attcaaagtt gatgaaaggt ggctcggttg tgatcgggct aggtggcaag
    12541 aaggcaataa aggtcagcgc accaatggtg ataatgggcg ctgtttcacc caaggcacgc
    12601 gccatgccga taataatacc ggtcaagatg ccaccgtaag agtatttgaa tacatggtcg
    12661 tacacgactt gccatttggt tgcaccaacc ccataggccg cttcacggat ggagacggga
    12721 atggcgcgga tggcttcacg cgtagacaca atcacaatcg ggagaatcag caggcctaag
    12781 gttaaaccgg cggtgaggat gctctggcca aagtccagca tgtagacaaa caggcctaat
    12841 gccagcaacc cataaatgat ggaagggaca ccggccaggt tagccacgtt aatttcaatc
    12901 aggtcggtaa accagttttt gggcgcatat tcttcaaggt atagacccgc agctacgccc
    12961 accggcaccg ctgtcagaaa cgtcactgac atcactagca gagaacctac ccaggcggac
    13021 agtaagccgg cacgacctgc acggcgcgaa ggaaactcgg tcaggaagtc caggctgaat
    13081 ttcgggtagc cgtctgcgat cagttgccag aacagcaaca tcagtgtaac caggcctagc
    13141 atcaggcata gcaggcctat cgcagcaaat agtgtgtcat tgcgcttatg gcgtttgatc
    13201 atggcgcgta catcggccag attttcgagg acagaagctt tatgttccat ggtgtgttgt
    13261 ttccattgac cggtagtcag gctaccggca gtgtagggtg gttatgtgtc agtaatgaga
    13321 caatcgtcag cgtttgatga atacgcttaa tagctttcac ggtatttttt acgtacccag
    13381 tgtcccacaa tattgaatga caatgtcatt gccatcaaca ccaggccggc cgcaaaaata
    13441 ctttggtaac caatgctacc atgcggtagg tcacccatgg caacttgtac gatataggca
    13501 gtgatggtcg ctgcgctatc cagtgggtta aatgtcaagt taggctgttg gcccgccgca
    13561 atcgctacca ccatagtttc acccacggca cgtgaaatcg ccaggatata ggctgccaca
    13621 atcccggaga ccgcagcagg cgtaacaact cggatcgcag tctggaagcg ggtagcgccc
    13681 atggcataag agccttcacg catactcata ggcaccgcac gcatcgcgtc ttctgacatg
    13741 gaagcgatgt aaggaataat catgatcccc atcacaatcc cggcacccag catattgaag
    13801 cttggtaaat cagggtaaat actttgcagc aatggggtca caaaaaacaa tgcaaagtaa
    13861 ccaaatacca cggttggtac acccaccagt aattcaagga taggcttgat ggtttcgcgg
    13921 gttctatgtg aagcaaattc ggacaggaaa atggcggaaa tggtgccaat cggcacagcc
    13981 actgccaacg caatcgccga agtagtcagg gtgcctgaaa ccagcggcat aatcccgtag
    14041 tgcgcgtctt caaacaatgg cgtccattgc gtatcggtga agaactctac gacagatacg
    14101 gtcttgaaga aactcaagga ctcgtacagc aagaccccga caatcgcgaa ggtggttgca
    14161 accgctacca gggcagctaa catgaggatc agctcaataa tgcgttcttt cacattccgg
    14221 cggatgttct tggccagtct ggggctgatg gtcagttgcg caggtgcttg cattgcttgg
    14281 gtcgccatag tgtttatttt gtcaggaagg aattttagcg gattatccgc agttcaggcc
    14341 gaatcactac taaaaaaggg gcattgctgc ccctttttat gattacttga ttcgggacaa
    14401 caagtcggtg atcttgatgc cgacttcaga tgaaccgtta aaaccagtgc ctggtttcat
    14461 ggctttgaag tgctctttca ctgcggtgta atcagctttt ggcagtggga cgtatttcac
    14521 ttccttcact aactctggcg cgttttccag gtagtagttc acaaacgctt tcacctcagg
    14581 cctgaaggcc gcagaagtcg cgtttacgta gataaacagt gggcgtgata atggctggta
    14641 agtaccatcc atcactgttg cttcggaagg cagaactgct ttaccacctt ctttagcaac
    14701 gactggcact gctttcagtt tgtcggtatt ttccagatag taagccaggc caaaataagc
    14761 catgccacct acgttaccgg atacaccttg taccagtacg ttgtcatctt ctgaaggagt
    14821 gtagtctgta cggctggctt tggccttgcc gttaacggct tctgtgaagt agtcaaatgt
    14881 accggaagct gtgcctgggc cgtacaagtt gattggtttg tctgggtaag tcgcattcac
    14941 ttctttccag ctcttaactt ttgagcctgg ctcccagatt tttttcagct cagctacagt
    15001 gatttgttta atccagtcgt tcttgccgtt caccaccacg gtcaacgcgt cataggcaat
    15061 tggcaattcg ataaactgca cgccgccttc tttacaagca tcgatttctt tttgtgagat
    15121 tgggcgcgaa gcatc
//
LOCUS       DATMSK010000243         4064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_442111,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000243 DATMSK010000000
VERSION     DATMSK010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442111"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1049
                     /locus_tag="VNZ84_08105"
     CDS             132..1049
                     /locus_tag="VNZ84_08105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWU66958.1"
                     /translation="MINIIIVSEKMAKPKTLGVFESLGMILALILVPVVLTLLFITPQ
                     QVIKSQGVKAMLPPHLRGAMLSTQSHLDAYAKQIGELQARILRLDAQNQRLSKLAGVK
                     STAEPINERTPVNEVPLNAPLGQGGPLVKDSPLSEEDLKATIEQMSADLDFRDEYQGK
                     IEAKLLQKSVLREMLPNSSPVEAAYSSSSYGWRIDPLSGHRAFHEGLDFPASVGMPVY
                     AAADGIVTTAVSTPDYGNLLKIEHGSGLETRYAHNSKLLVKAGERVTKGQKIALIGST
                     GRSTGPHLHYEIRLNGNPLDPREYLRNRS"
     gene            1154..3865
                     /gene="secA"
                     /locus_tag="VNZ84_08110"
     CDS             1154..3865
                     /gene="secA"
                     /locus_tag="VNZ84_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWU66959.1"
                     /translation="MLASLFKKVFGSRNDRLVKQYQQIVKQINALEPEVQALTDEQLR
                     AKTEEFKQRYTQGETLEKLLPEAFAVVREGGKRALGMRHFDVQLIGGMVLNAGKIAEM
                     RTGEGKTLVATLPTYLNALTGKGVHVVTVNDYLAKRDAEWMGKLYNFLGLSVGINLSQ
                     MPHDAKQQAYAADITYGTNNEFGFDYLRDNMVFSKEERVQRPLHYALVDEVDSILIDE
                     ARTPLIISGQAEHSVDLYGAINEVAKQLVRQQVEDGEGDFWVDEKAQSVTMSEVGHEH
                     AEQLLEQTGLLTAGSSLYEPANITLVHHLYASLRAQNLYHRDQHYVVRNGEIVIVDEF
                     SGRMMPGRRWSDGLHQAVEAKEGVTIQKENQTLASITFQNYFRMYQKLSGMTGTADTE
                     AYEFNQIYGLETVVIPTHRPVQRIDAMDKVYRTGMEKYKAVIEDIKQCQAKGQPVLVG
                     TTSIENSELISKLLTQAKLEHQVLNAKQHEREATIVAEAGRPGVITIATNMAGRGTDI
                     VLGGNSENEIHSIQQDEKLSEADKTAKVAQLKAEWQQRHDAVLAAGGLHIIGTERHES
                     RRVDNQLRGRAGRQGDPGSSRFYLSLEDPLLRIFSGERVAAIMTRLNMPEGEAIEHAM
                     VTRAIENAQRKVEGRNFDIRKQLLEYDDVSNDQRKVIYEQRNELLESVDVGQTIRAMR
                     EDVINSLFNTYVPPGSVEEQWEIPALEQALQAELSIGLPVAEWLENNPDLHEETLRER
                     ILNAANESYASKEAMAGNDNMRNFERAVMLQSLDNHWREHLAALDHLRQGIHLRSYAQ
                     KNPKQEYKREAFELFESLLNTVKSEVTKIIMQVQVRSREELEEEERRAEEALRAAEVG
                     NLQYQHTDYNADATASAELPEGVRVGRNDPCPCGSGKRFKNCHGALS"
     gene            3890..>4064
                     /locus_tag="VNZ84_08115"
     CDS             3890..>4064
                     /locus_tag="VNZ84_08115"
                     /inference="COORDINATES: protein motif:HMM:NF018630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HWU66960.1"
                     /translation="MSDQEIPSPCVGVCSIDEGNGFCQGCFRTLEEIRQWWDLDNAGK
                     AEVIKLANARESAV"
BASE COUNT         1047 a          920 c         1151 g          946 t
ORIGIN      
        1 gtgatgatgt ttgaagatct gattgacttt gaacatggcg ggttgtcaac tgaatgcttg
       61 aatattgtac accagcaaga cggcgagtgt ttgcagtcgt ttaggcgcgg tgcaaagcga
      121 gagttcgtag catgattaat atcattattg tttcagaaaa aatggccaaa cccaaaacgc
      181 tgggcgtgtt tgagagcttg ggcatgatat tggcgctgat cctggtacct gtggtgctca
      241 ctttgttgtt tatcacacca caacaagtca ttaaatccca aggcgtgaag gcgatgttgc
      301 caccacattt acgtggtgcc atgttgtcta cacaaagcca tctggatgct tatgccaagc
      361 agattggtga gttgcaggcg cggatattgc ggctggatgc ccaaaaccag cggctatcca
      421 agttggcggg cgttaaatcc actgccgaac ccatcaatga gagaacacct gtgaacgaag
      481 tgccgctgaa tgcacctttg ggtcaaggtg gcccattggt taaagattct cccttaagtg
      541 aggaggatct gaaagcaacc attgaacaaa tgtcggctga cctggatttt cgggatgaat
      601 accagggcaa aatcgaagcg aaactgttgc aaaaaagtgt tttgcgcgaa atgttgccta
      661 acagcagccc ggtcgaggcg gcttacagtt catccagcta tggttggcgt attgatccct
      721 tgagtgggca tcgggcattt catgaagggt tggattttcc tgcatcagtt ggaatgcctg
      781 tgtacgcagc ggccgacggg attgtgacca cggcagtcag tacgcctgat tatggcaatt
      841 tgctcaagat tgagcatgga tctggcttgg aaacacgcta tgcgcataac tccaaattac
      901 ttgtgaaggc cggggagcgt gtcactaagg gtcagaaaat cgcccttatt ggtagtaccg
      961 gccgttctac agggccgcat ttgcattatg aaatccgcct caatggcaac ccgctggatc
     1021 cgcgcgaata cctcaggaac cgctcctgag taaaatactc atcatgcaat aattgagaca
     1081 atccgcctct atatcttata caatctcagt ttatttcaat ttaacgccgc gcaagcggaa
     1141 atcggtgtga ctcatgttag cctcgttgtt taaaaaagtc tttggcagtc gcaatgatcg
     1201 actggtcaag caatatcagc aaatcgtcaa acaaatcaac gcgttggagc ctgaggtaca
     1261 ggcgctcact gacgagcagt tgcgggcaaa aaccgaagag ttcaaacaac gctatacgca
     1321 aggtgagacg ctggaaaaac tgttgcccga ggcctttgcc gttgtccgtg aaggcggtaa
     1381 gcgtgcactg ggcatgcgtc attttgatgt gcagctgatt ggtggcatgg tcctcaatgc
     1441 gggcaaaatt gccgaaatgc gtactggtga aggtaaaaca ctggtagcga ccttgccaac
     1501 atacctgaat gccttaactg gtaaaggtgt gcacgtcgtg actgtgaacg attacctggc
     1561 caagcgagat gcggaatgga tgggtaagct gtacaacttc ctgggcttga gcgtggggat
     1621 caacctgtcg caaatgccac atgatgccaa gcaacaggcc tacgccgctg atattaccta
     1681 cggtaccaat aacgagtttg gttttgacta cctgcgggac aatatggtgt tcagcaagga
     1741 agagcgtgtg caacgcccct tgcactatgc gctggtggat gaagtggact ccatcctgat
     1801 tgatgaagcc cgtacaccgc tgattatttc gggccaggca gaacacagtg tggacctgta
     1861 tggtgcgatc aatgaggtgg ctaagcaatt ggtacgtcag caggtcgaag atggtgaggg
     1921 cgatttctgg gtcgacgaaa aagcccagag cgtcactatg agcgaagtgg gtcacgagca
     1981 tgcagaacag ttgttggagc aaaccggact gttaacggct ggttccagtt tgtatgaacc
     2041 tgccaatatc acactggtac accacctgta tgcctcgttg cgtgcacaaa acctgtacca
     2101 tcgtgaccag cattacgtgg tgcgtaatgg cgaaatcgtg atcgtcgatg agttttctgg
     2161 ccgtatgatg cctggtcgcc gctggtcaga tggtttgcat caggcagttg aagccaagga
     2221 aggcgtgacc atccagaaag aaaaccagac cctggcatcg attacgttcc agaactattt
     2281 ccgcatgtat caaaaactga gcggcatgac cggtactgca gataccgagg cctacgagtt
     2341 taaccagatt tacggtctgg aaacggtggt gattccaacc catagacctg tgcagcgtat
     2401 tgatgccatg gacaaggtgt atcgcacggg gatggaaaaa tacaaggcgg tcattgagga
     2461 catcaagcag tgccaggcta aaggccagcc ggtcctggtg ggcacgacct ccattgaaaa
     2521 ctccgagctg atttccaagt tgttgacgca agccaagttg gagcatcagg tgctcaatgc
     2581 caagcagcat gagcgtgagg cgaccattgt ggcagaagct ggtcgtcctg gcgtaattac
     2641 cattgcaacc aacatggccg gccgtggtac cgatattgtg ctgggcggta attccgaaaa
     2701 tgaaattcac agcattcagc aagatgagaa attgtctgag gctgataaaa cggcaaaggt
     2761 cgcgcagttg aaagccgaat ggcagcagcg tcatgatgcg gtattggctg cgggtgggtt
     2821 gcatattatc ggtaccgagc gtcacgagtc tcgccgtgtc gataatcagc tccgtggccg
     2881 tgccggtcgc cagggggatc cgggctccag ccgtttctac ctgtcactgg aagatccgct
     2941 gttgcgtatt ttctctggcg agcgtgtggc cgcgattatg acacgcctca atatgccaga
     3001 aggcgaagcg attgagcatg ccatggtgac gcgtgcgatt gagaatgcac aacgcaaggt
     3061 tgaaggccgt aactttgata tccgtaagca attgctggaa tacgacgatg tttctaacga
     3121 ccaacgcaag gtcatttacg agcagcgtaa tgagttgttg gagtccgtcg atgttggcca
     3181 aacgattcgt gccatgcgtg aagatgtgat caacagcctg tttaatacgt atgtgccgcc
     3241 tggcagcgtt gaagagcaat gggagatccc tgcgcttgaa caagccctgc aggcagagct
     3301 gtctatcggt ctgcccgtgg ccgaatggct ggaaaacaac ccggacctgc atgaagaaac
     3361 cttgcgtgag cgtatcctga atgctgctaa cgagagctat gcctcaaaag aggccatggc
     3421 tggcaatgac aatatgcgta acttcgagcg tgctgtcatg ctgcaaagcc tggataacca
     3481 ttggcgcgag catctggcgg cgctggatca tttgcgccag ggtatccatt tgcgctctta
     3541 tgcccagaag aaccccaaac aggaatacaa acgtgaagcg tttgagctgt ttgagtcact
     3601 gttgaatacc gtcaagagtg aggtaaccaa gatcatcatg caggtacaag tgcgctcacg
     3661 cgaagagctt gaagaggaag agcgtcgtgc ggaagaggcg ttgcgcgccg cagaagtggg
     3721 taatttgcag taccagcaca cagattacaa tgctgacgct actgcatcgg ccgaattgcc
     3781 tgaaggggta cgtgtagggc gtaatgatcc ctgcccgtgt ggttctggta aacggtttaa
     3841 gaattgccat ggtgccttga gttaagctgg gtacgtgaaa ggaaacaaga tgtcagacca
     3901 ggaaattcca tctccatgtg taggggtttg cagtattgat gaaggcaatg gattttgcca
     3961 gggctgtttt aggaccttgg aagaaatccg tcaatggtgg gaccttgata acgcaggcaa
     4021 agccgaagtg attaaattag cgaatgcacg cgaatcggcg gtat
//
LOCUS       DATMSK010000244         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_278734,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000244 DATMSK010000000
VERSION     DATMSK010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278734"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2238
                     /gene="topA"
                     /locus_tag="VNZ84_08120"
     CDS             <1..2238
                     /gene="topA"
                     /locus_tag="VNZ84_08120"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWU66961.1"
                     /translation="FNEITQTAVKHAIDHPRDISMDLVNAQQARRALDYLVGFNLSPL
                     LWKKIRRGLSAGRVQSPALRLIVERELEIEAFTSQEYWSIHMSALKHAHGFDAKLIQL
                     NGQKVEQFTVTNHDQQADIVGKLLLASAGKTTVTRVEKKQRSRSPAAPFTTSTLQQEA
                     VRKLGFTTSRAMRVAQQLYEGMDVGSGTVGLITYMRTDSFSLATEAVMQIRDYVKKHF
                     DADYLPKAPIMYKTKAKNAQEAHEAIRPTDITRTPASVRQYLNDEQFKLYEMIWKRTL
                     ACQMTQAKYDAVSVDLAVGSEANLFRASGQTLVFPGFIAVYMEGKDDEEDEDAEAKLP
                     HLETGEVLTVEKIFGEQHFTEPPPRYSEASLVKALEEYGIGRPSTYASIISTLQDREY
                     VLLDKKRFTPTDVGRVVNKFLTEHFTQYVDYDFTAKLENELDEIAEGNLDWVPVMDKF
                     WQGFNQQIQVKADVERPGNELIDEFCPKCGRQLQKQLSRYGSFIGCTGYNATPKCDYK
                     RNLDGEVNNASEPTVIGLDAETQKEIYLMNGPYGPYLQVGQPVEGEKKKPKRVSIPKE
                     IPLSQVNIDTAHMLLSLPRDLGQHPETGKKIVANIGRFGPYVNHDGKFKSIPKTESVF
                     SIDLEGAVKLLAAASGPAPLADLGEHPSGEGRIEVFSGRFGPYVQHNGIRATLPKSVT
                     PEELTVEQALELLAEKAAKEGGKTTSKKATAKKTATKKAPAKKAASTKTATASSKRTK
                     KAAEE"
     gene            complement(2283..>3515)
                     /locus_tag="VNZ84_08125"
     CDS             complement(2283..>3515)
                     /locus_tag="VNZ84_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HWU66962.1"
                     /translation="DVRMGMFIHELKGPWMDHPFWSSQFVLSDPQDLKKLLASPIKQV
                     VIDTRWGLDVESSQDETVESTQMTDKEMAMLELDGPVDDSQPFYNAPVFNPGTLKKTT
                     LEAEREQAAKIIASSRNAIASMFKDLRMGKAIETAAVMPIVEEISASVERNVHALISL
                     VRLKTADDYTYMHSVAVCALMTALARQLQLSDAEVKQAGLAGLMHDMGKAGIPLQILN
                     KPGSLTDNEFSVVQEHPKLGYDMLVKANISDPVTLDVCLHHHEKIDGSGYPEKLSSDQ
                     ISLFAKMGAICDVYDAVTSTRAYKPAWEPGIALKRMASWNGHFDPVVFKAFVKSLGIY
                     PIGTVVMLKSGRLAVVVRQSPGSLLKPVVKAFFSTKSQMHLPVVELDLSKNVDEIIGN
                     ESVATWGIGNIDHLWSNH"
BASE COUNT          956 a          909 c          866 g          784 t
ORIGIN      
        1 ttcaacgaaa ttacgcaaac cgcggtgaaa cacgcgattg accacccacg ggatatctcc
       61 atggatctgg tcaatgctca acaagcccgc cgcgcattgg attatctggt cggtttcaat
      121 ttatcccctt tattgtggaa aaaaatccgc cgcggtttat ctgctggccg tgtacaaagc
      181 ccagccttgc gcctgattgt ggagcgagaa ctggaaattg aagcgtttac cagccaggaa
      241 tactggagca tccacatgag tgcgctgaaa catgcgcacg gctttgatgc caaactcata
      301 caactcaatg gtcaaaaagt agaacagttc acggttacca atcatgacca gcaggcggat
      361 atcgttggca agctattgct ggcttctgca ggtaaaacca ccgtcacccg tgttgagaag
      421 aagcaacgta gtcgcagccc ggctgcgccg ttcaccacgt caacactgca acaggaagcc
      481 gtgcgcaagc taggctttac caccagccgt gccatgcgtg tggcacaaca gttatacgaa
      541 ggcatggacg tcggaagtgg caccgtcggt ttgatcactt atatgcgtac agactctttc
      601 agcctggcaa ctgaagcggt gatgcagatt cgtgactacg tgaaaaagca ttttgatgca
      661 gactacctgc ccaaagcgcc catcatgtac aaaaccaagg caaaaaatgc gcaagaggcg
      721 cacgaggcga ttcggccaac agacatcacc cgcacaccag ccagcgtccg ccaatatttg
      781 aatgacgaac aattcaaatt gtatgaaatg atctggaagc gtaccctggc ttgccagatg
      841 acgcaggcaa aatatgatgc cgtcagtgtt gacctggctg tcggcagtga agctaacctg
      901 ttccgcgcca gtgggcaaac cctggtattc ccgggcttta tcgcggtgta tatggaaggc
      961 aaagacgatg aagaggacga agacgcggaa gccaagctac cgcacctgga aaccggagaa
     1021 gtactgacgg ttgagaagat ttttggtgag cagcacttta ccgagcctcc accgcgctac
     1081 tcagaagcca gcctggtgaa agcactagaa gaatatggta ttggtcgccc ttcgacttac
     1141 gccagcatta tttctaccct gcaagatcgc gaatacgtct tgctggataa aaagcgcttc
     1201 acgccgaccg atgttggccg tgtggtgaat aaattcctga ctgagcattt tacccaatac
     1261 gtggattatg actttacggc caagctcgaa aacgagctgg atgaaattgc cgaaggcaat
     1321 ctggactggg tgccggtcat ggacaagttt tggcaaggat tcaaccagca gattcaagtg
     1381 aaagccgatg tggaacgccc tggcaatgag ttgatagacg agttctgccc gaaatgcggc
     1441 cgccagttgc aaaaacaact cagccgttat ggtagtttta tcggctgtac tggctataac
     1501 gcaacaccca aatgcgatta caaacgcaat cttgatggtg aagtcaacaa tgccagcgag
     1561 cccaccgtga ttggcctgga tgcggaaacg cagaaagaaa tctacctgat gaatggtccc
     1621 tatggcccct atttgcaggt tggtcagcca gttgaaggcg aaaagaaaaa acccaagcgc
     1681 gtcagtattc ccaaagaaat tccgctgagc caggtcaata tagatactgc acatatgctg
     1741 ttaagcttgc cacgcgactt ggggcaacac ccggagaccg gtaaaaagat tgttgccaat
     1801 atcggtcgtt ttgggcctta tgtgaatcat gatggcaagt ttaaatccat cccaaaaacc
     1861 gaaagtgtat tcagcataga cctggaaggt gcggtcaagt tactcgcggc cgccagtggt
     1921 ccggcaccgc tggccgacct gggagagcat ccgtcaggtg aaggacggat tgaggtattt
     1981 tcaggccgct ttggacctta cgtgcagcat aatggtattc gtgcaacatt gcccaaaagc
     2041 gtgacaccgg aagaacttac ggtagagcag gcattagagc tgctagccga aaaagctgcc
     2101 aaagaaggtg gcaaaaccac gagcaagaaa gcaacggcaa agaaaaccgc tacaaaaaaa
     2161 gcgcctgcca aaaaagctgc atcaacgaaa acagcaaccg cctccagcaa acgtaccaag
     2221 aaagcggctg aagagtaaca cacgcgtcaa accagggcgc atgcgcgctc tgtctcgctt
     2281 ggtcaatgat ttgaccacaa gtgatcaata ttgcctatgc cccaggtggc gacagactcg
     2341 tttccgataa tttcatccac atttttcgac aaatccagct cgacgaccgg gagatgcatt
     2401 tgcgatttgg tagaaaaaaa tgcctttact accggtttga gcaagctacc cgggctttgc
     2461 ctgaccacga cggccaggcg accggacttc agcatcacca cggtaccgat tggataaata
     2521 cccaggcttt tgacaaatgc cttgaacacg acgggatcaa aatgcccgtt ccagctcgcc
     2581 atgcgtttga gcgctattcc cggctcccac gccggcttgt aagcccgcgt agaagtcact
     2641 gcatcataca cgtcacagat tgcacccatt ttggcaaaca ggctgatctg gtctgaagag
     2701 agtttctcag gatagcccga gccgtcgatc ttttcatgat gatgcaagca cacatccagg
     2761 gtaacaggat ctgaaatatt cgctttcacc agcatgtcat agccgagttt gggatgttct
     2821 tgaaccacac tgaactcgtt atccgtcaag gagcctggtt tatttagaat ctgcaacgga
     2881 atgccggctt tacccatgtc gtgcatcaac cctgccagcc cggcttgttt cacttccgca
     2941 tccgatagct gaagctgcct ggcaagcgcc gtcatcagcg cacagacagc aacagaatgc
     3001 atataggtat agtcatctgc tgttttgagg cgtaccaggc taatcaaagc gtgtacattg
     3061 cgttcaaccg aggcagaaat ctcctctaca attggcatga cagctgctgt ctcaatcgct
     3121 tttcccatgc gcaaatcttt aaacatggag gcgattgcat ttcgtgaaga ggcgatgatt
     3181 ttggctgctt gctcacgctc cgcctcgagt gtggttttct tcaacgtccc aggattgaat
     3241 acaggcgcat tatagaatgg ctgactgtca tcaacaggac catctaattc cagcatggcc
     3301 atttctttgt ctgtcatttg cgttgactca acagtttcgt cttgagaact ttcaacatct
     3361 agcccccagc gagtatcaat caccacttgt ttgatagggc tggcgagcag tttcttcaaa
     3421 tcttgcgggt cactcagcac aaactgactg ctccagaagg gatgatccat ccacgggcct
     3481 ttgagctcat gtataaacat tcccatccgc acatc
//
LOCUS       DATMSK010000245         6392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_372136,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000245 DATMSK010000000
VERSION     DATMSK010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372136"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VNZ84_08130"
     CDS             complement(<1..252)
                     /locus_tag="VNZ84_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HWU66963.1"
                     /translation="MQMKASLLAAALCGVLSGQAFADHETIKPKAYDSLGKCVKAALS
                     KHDGKIVKVEAKSERKQLVYEFDVQSNDGKAWDIECNAKT"
     gene            complement(431..1225)
                     /locus_tag="VNZ84_08135"
     CDS             complement(431..1225)
                     /locus_tag="VNZ84_08135"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HWU66964.1"
                     /translation="MQQYHDLLNHVLAHGHVKTDRTGTGTVSVFGYQMRFNLAEGFPL
                     LTTKKVHLKSIIHELMWFLQGSTNIAYLKENGVSIWDEWADEEGNLGPVYGYQWRNWP
                     KPDGSHIDQITQVVNQIKKTPDSRRLIVSAWNVADVERMKLPPCHAFFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMVAQVCDLQLGDFVHTLGDAHIYSNHMEQVKE
                     QLSRTPRALPNMRINPEVKDIFAFKFDDFTLENYDPYPAIKAPVAV"
     gene            complement(1255..2688)
                     /gene="thrC"
                     /locus_tag="VNZ84_08140"
     CDS             complement(1255..2688)
                     /gene="thrC"
                     /locus_tag="VNZ84_08140"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWU66965.1"
                     /translation="MKYISTRGQSPALTFSEILLGGLAPDGGLYLPEQYPQFSDADLN
                     AMRGMNYRDLAFAILSRLVDNSDIPHADLKAIIDKTYTAEVYGYARAGQNAEDITPTL
                     KLEDNLYLLSLSNGPTLAFKDMAMQLLGNLFEYLLAKTGQTTNILGATSGDTGSAAEY
                     AMRGKKGVRVFMLSPYQKMSRFQTAQMFSLQDHNIFNIAVKGVFDDAQDMVKAVSNDH
                     AFKAQYKIGAVNSINWGRIAAQVIYYFKGYLAVTSSNSQKVSFTVPSGNFGNICAGHI
                     ARMMGLPIDQLVVATNENDVLDEFFNTGVYAPRGSANTYHTSSPSMDISKASNFERFV
                     YDLVGQDSARVRELWTAVDHGGKFDLNAEGYFDKVSDFGFTSGHSNHANRMQTIRATK
                     KKYGVTIDTHTADGLKVALEHRKANVPMLVLETALPAKFEDAIVEALGEVPERPASLV
                     GLENLPQKSTVMDVSVDAIKAFIAANT"
     gene            complement(2703..4016)
                     /locus_tag="VNZ84_08145"
     CDS             complement(2703..4016)
                     /locus_tag="VNZ84_08145"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWU66966.1"
                     /translation="MKELKVGLLGIGTVGGGTYTVVTRNQADISRRIGGNIRIVQVAD
                     RNLELAKQVTGGQVAVTDDAFAVVNNPEVDVVVELIGGYTVAKELVLRAIANGKHVVT
                     ANKALLAVHGNEIFKAAAEKGVILAFEAAVAGGIPIIKALREGLSANRIEWVAGIING
                     TTNFILTEMRDKGLAFADVLKEAQRLGYAEADPTFDVEGIDAAHKLTIMASIAFGMPV
                     QFDQAYTEGITKLTIQDIQYAEELGYRIKLLGVSKKTATGVELRVHPTLIPEKRLIAN
                     VDGAMNAVVVKGDAVGPTLYYGAGAGAEPTASAVVADLVDIARLQGTTAEQRVAFLGY
                     QLDQQQALPVLPIAEVQSAYYLRMHASDKPGVMADVTKILADLQISIDAMLQKEPQEG
                     EVEADIVILTHTTQEKTMDAAIAKIEALPAIAGTVIKIRMEALSR"
     gene            complement(4070..5296)
                     /locus_tag="VNZ84_08150"
     CDS             complement(4070..5296)
                     /locus_tag="VNZ84_08150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWU66967.1"
                     /translation="MKPVNKSSKLSNVCYDIRGPVLQRARQMEEDGQRIIKLNIGNPM
                     PFGFNAPEEIVQDVIHNMDQASGYTDSKGLFAARKAVMHYTQQKNIAGVTIDDIIIGN
                     GVSELIVMAMQGLLNNGDQILVPMPDYPLWTAAVNLAGGTARHYVCDEQSGWLPDLKD
                     IENKITANTKGIVIINPNNPTGALYPKETLEGIIEIARHHGLVIFADEIYDKVLYDGN
                     THTSIASLADDVLFVTFNGLSKNYRACGYRSGWMIISGDKKDAKDYIEGLNMLASMRL
                     CANVPGQLAIQTALGGYQSINDLVAPSGRLCKQRDLAYDMLTSMPGVTCVKPAAAMYL
                     FPKLDPVMYPIEDDQQFILDLLLEEKVLLVQGTGFNWKAPDHFRVVFLPNIDDLTEAM
                     TRIGRYLDSYRKKHAK"
     gene            5457..5840
                     /locus_tag="VNZ84_08155"
     CDS             5457..5840
                     /locus_tag="VNZ84_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HWU66968.1"
                     /translation="MKLHLHTSEQQYQINGVDSDAVTINRQRHEQAVIVSEQALSTDW
                     FQGAWEALDAQALSAILDFEPEVVLLGTGDKQRFIHPKHTQSFLSGNIAVECMTTAAA
                     CRTFNILSAEGRKVVAALLLEKHLK"
     gene            complement(5857..>6392)
                     /locus_tag="VNZ84_08160"
     CDS             complement(5857..>6392)
                     /locus_tag="VNZ84_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HWU66969.1"
                     /translation="TSFFILLVSLLLPGCASMGERKVSMSTAQLQQKLNSKLAKPLTV
                     LKVFHVQLSNAQVSMDPASGRIHALMDTSVQSDLLANTASGKLGVSGLIKFDQALNAV
                     VLDQPAVDSFQLDDANSEWGGLVQQLAKEMGGKWLNQLVLYEVKPEELSHGGQRYQPS
                     DLKVTADGLQVTLKPQQ"
BASE COUNT         1614 a         1677 c         1460 g         1641 t
ORIGIN      
        1 tgtttttgca ttgcactcaa tatcccaggc cttaccatcg ttagattgca catcaaattc
       61 atacaccaat tgtttacgtt cagatttggc ttctacctta acgattttgc catcatgctt
      121 ggacaaagcg gcttttacgc atttgcccag gctatcgtaa gcctttggtt taatggtttc
      181 gtggtcagcg aatgcctgac cggataatac accgcacaac gcggctgcca ataatgacgc
      241 tttcatttgc ataatgatct ccttgtttaa agtcatgaac gaaagttttg gaacaggcgc
      301 taggacgcgc tattttaaac atccacaacg ctgagaagga tatcaaaaag ggaataagct
      361 tttgaaaata ttacacttgg ttaacaaaat ggctaaaaat agccattttg ttcgggcaaa
      421 gcctgctggc ctacacagct actggtgcct taatggcagg ataagggtcg taattttcta
      481 gtgtgaagtc gtcaaattta aacgcaaaaa tatcctttac ttcaggatta atgcgcatgt
      541 ttggcaaagc ccgtggggtg cgtgacaact gctctttgac ttgctccata tgattgctgt
      601 agatatgggc atcacccaat gtgtgcacaa aatcgcccaa ctgcaaatcg cacacttgcg
      661 ccaccatcat ggtcaataac gcataagacg caatattaaa tggcacaccc aaaaagatat
      721 ctgcactacg ctggtataac tggcaactta acttaccatc tgccacataa aactggaaaa
      781 atgcgtgaca cggtggcaat ttcatacgct cgacatcggc cacattccag gccgagacaa
      841 tcaggcgacg cgaatctggt gtcttcttga tctggttgac tacctgggta atctggtcga
      901 tgtggctacc atctggctta ggccagttgc gccattgata gccataaacc gggcccagat
      961 tgccctcttc atcggcccac tcgtcccaga tactgacgcc attttctttc aggtatgcaa
     1021 tattggtact gccctgcaag aaccacatca gctcatgaat aatagacttc aggtgcacct
     1081 ttttcgtggt aagtaaagga aagccttctg ccaggttaaa ccgcatctga tagccaaaca
     1141 ctgacacggt accggtgccg gtgcgatcgg ttttgacgtg accatgcgca agtacgtggt
     1201 ttaataaatc atgatactgt tgcatgagat gctcctcaat tcgcttattt ggtcttaggt
     1261 gttagcggca ataaacgcct tgatcgcatc aacgctaaca tccattacgg tggacttctg
     1321 aggcaagttc tccaggccaa ccagactggc tgggcgctca ggcacctccc ccaatgcctc
     1381 tacaatcgca tcttcaaact tggctggcaa ggctgtttcc aataccaaca tggggacatt
     1441 cgccttgcgg tgttctaatg ccactttcag gccatcggcg gtatgagtat caatcgtcac
     1501 gccatatttt ttcttggtcg cacgtatggt ttgcatgcgg ttagcatgat tgctgtgccc
     1561 ggacgtgaaa ccgaaatcag acaccttgtc gaaataccct tcggcattca ggtcgaattt
     1621 gccaccatga tccaccgccg tccataattc acgtacccgc gcactgtctt gccctaccaa
     1681 gtcatagaca aagcgctcaa aattagaagc tttgctgata tccatggatg ggctggaagt
     1741 gtgataagta ttcgctgaac cacgtggcgc atagacaccc gtattaaaaa actcatccag
     1801 cacatcgttc tcattggtcg cgacaaccag ttgatcaatg ggcaatccca tcatgcgtgc
     1861 aatatggcca gcacagatat tgccaaagtt accgctaggc accgtaaagc tgactttctg
     1921 gctgttgctg ctcgtcaccg ccagatagcc tttgaagtag taaatcacct gggcagcaat
     1981 acggccccag ttaatggagt tgacagcacc aattttgtat tgcgccttga atgcgtgatc
     2041 gttggacact gctttgacca tgtcctgcgc atcatcaaac acgcctttca cggcgatatt
     2101 aaaaatattg tggtcttgca ggctgaacat ttgtgccgtc tggaaacggc tcatcttctg
     2161 gtaaggcgac agcatgaata cacgcacacc ttttttaccg cgcatggcgt actcagccgc
     2221 agagccggta tcgccagaag tggcgcccag gatattggtc gtttgaccag ttttggccaa
     2281 caaatattca aacagattgc ccagcaactg catggccatg tctttaaacg ccaatgttgg
     2341 accgttagaa aggcttagca ggtagaggtt atcttcaagc ttgagcgtag gcgtaatgtc
     2401 ttctgcattt tggccagcac gcgcatagcc atagacttct gccgtgtagg ttttgtcgat
     2461 aattgctttt aagtcggcgt ggggaatatc actgttatcc acgaggcgag acagaatggc
     2521 gaatgccagg tcgcggtaat tcatgccacg catggcgttc aaatcggcat cactgaattg
     2581 cggatattgc tcaggcaggt ataaaccacc atctggcgcc agtccaccca gcaaaatttc
     2641 actaaatgtt aaagcaggcg attgcccacg ggtactgata tatttcattg tgttgaacct
     2701 aattagcgtg acagcgcttc catgcggatt ttgataacgg taccggcaat cgcaggcagg
     2761 gcctcaatct tggcgatggc tgcatccatg gttttttcct gtgttgtatg tgtcaggatt
     2821 acaatatcag cctcaacttc gccctcttgt ggctccttct gcagcatggc atcaattgat
     2881 atttgcaagt cagccagaat cttggtcaca tccgccatga cacctggttt atcagaagca
     2941 tgcatgcgca aatagtatgc agactgcacc tcggcaatcg gcagtacagg aagcgcttgt
     3001 tgctgatcta actggtaacc caaaaaggcc acacgctgct ctgccgttgt cccttgcagg
     3061 cgtgcaatat ccactaaatc tgccactacc gcgctggctg tgggctcagc accggcaccg
     3121 gcaccgtaat acaacgttgg accaaccgca tcgcccttaa caactaccgc attcatggca
     3181 ccatcgacat tggcgatcaa gcgtttttcc ggaatcaagg tcgggtgaac ccgcagctca
     3241 acgcctgtgg ctgtcttctt gctgacacct aacaacttga ttctgtaacc aagctcctca
     3301 gcatactgga tatcctgaat cgtcagtttg gtaatgcctt cggtataggc ttggtcaaat
     3361 tgcacaggca tcccaaacgc aatgctggcc atgatggtca gtttgtgtgc ggcatcaatg
     3421 ccttccacat caaaggtcgg atccgcttct gcatagccca ggcgttgggc ttctttgaga
     3481 acatcagcaa atgccaggcc cttgtcacgc atttcagtca ggataaaatt ggtcgtgccg
     3541 ttgataatgc cagccaccca ttcaatccta ttcgcgctca agccctcgcg taatgccttg
     3601 ataattggaa taccaccggc aactgcggcc tcaaaggcca agatcacgcc cttctcggct
     3661 gccgccttga aaatttcgtt accgtgcacg gccagcaaag ccttattggc agtcaccaca
     3721 tgtttcccat tggcaattgc tcttaacact agctctttgg cgacggtgta gccgccaatc
     3781 aactctacga caacatcaac ctcaggattg ttgaccacgg caaacgcatc atcagtcacg
     3841 gcgacctgtc cacctgtgac ctgcttagcc agttccaggt tgcggtcagc gacttgcaca
     3901 atacggatat tgccgccaat acggcgcgag atatccgcct gattacgtgt gacgaccgta
     3961 taggtaccgc cacccactgt gcctataccc aacaatccta ctttcaattc tttcatagtt
     4021 atactctacg ttgatggcag ccgatggctc accttaattt gaatgttgct tattttgcat
     4081 gcttcttgcg atagctatcc aggtaacggc caatacgagt catggcttct gtcaggtcat
     4141 cgatattagg caggaacacg accctgaaat gatcaggtgc tttccaatta aagccggtcc
     4201 cctgcacaag cagcactttc tcttccagca acaaatccag gataaactgc tgatcatcct
     4261 cgattggata catgactgga tccagcttgg gaaacagata catggccgct gctggcttaa
     4321 cacaggtcac gcccggcatg gatgtgagca tgtcgtaagc caggtcacgc tgtttgcata
     4381 agcggccact gggtgcaacc aggtcattaa tactttggta gccaccaagc gctgtctgta
     4441 tcgccaattg accgggcacg ttggcgcaca ggcgcatgga agccagcata ttcaggcctt
     4501 cgatgtaatc tttggcatct tttttatcac cggagataat catccagcca gagcgataac
     4561 cacaggcgcg gtaattcttg gataaaccgt taaaggtcac aaacagcaca tcatccgcca
     4621 gcgaggcaat cgacgtatgc gtgttgccgt catacagcac cttgtcataa atttcgtcgg
     4681 caaagatcac caacccatga tgacgtgcaa tctcgatgat cccttccaac gtttctttag
     4741 ggtataaagc accggtcggg ttattcggat taataatcac gatgcctttg gtattagccg
     4801 tgatcttgtt ttcaatgtct ttcagatcgg gcaaccagcc tgactgttcg tcacacacat
     4861 aatggcgcgc cgtgccacct gccaggttca ccgcggccgt ccacaaggga taatctggca
     4921 tgggcaccag aatctggtcg ccattattga gcaggccctg catggccatc acgatcagct
     4981 cggatacacc attcccgatg atgatgtcat caatggtgac accggcaata ttcttttgct
     5041 gcgtgtagtg catgacggct ttgcgcgcgg caaacaaacc cttggagtcg gtatagccag
     5101 aggcctgatc catattgtgg atcacgtcct gaacgatttc ttctggcgca ttaaagccga
     5161 atggcatcgg gttaccgata ttcagcttga taatgcgctg cccgtcttcc tccatctggc
     5221 gagcacgctg taataccggg ccgcggatgt cgtaacagac attactgagt ttgctggatt
     5281 tattgactgg cttcatcgct ggttaacaaa cacatctggc gtgtgttaag ctcttaaagt
     5341 aaaactttca ttctacctgc aaacccttgc tacatcaatg aaccttcaga cttagccagg
     5401 gtctgcttgg aaacatatcc tgagcaattt tgcactgtgc gtcagaccgc gaacttatga
     5461 aactacacct gcataccagt gaacagcaat accaaatcaa tggcgtcgat agcgatgccg
     5521 tcactatcaa tcgccaacgc catgaacaag cggtgattgt cagtgaacag gcgttaagta
     5581 ctgactggtt ccagggagcg tgggaagcgc tagatgcgca agcattgtca gctatcttgg
     5641 atttcgaacc agaagtcgtt ttattgggaa ctggggataa acagcgcttt attcacccca
     5701 agcatacgca atcgtttttg tcagggaata tcgctgtcga atgcatgacg acagctgccg
     5761 cttgccgtac attcaatata ttatcagcag aaggccgcaa ggttgtcgca gcactcttgc
     5821 tggaaaaaca cttgaaataa tcacactggg aaaagattac tgctgcggtt tgagagttac
     5881 ctgcaagcca tccgcagtca ctttgaggtc agaaggttgg taacgttgcc cgccatgact
     5941 taactcttca ggcttgactt cataaagcac cagttgatta agccacttgc cacccatctc
     6001 tttggccaat tgttgcacca agccacccca ctcactattt gcatcatcca gctgaaaaga
     6061 atctacggca ggttgatcca gcacaactgc gttgagtgcc tggtcaaatt taatcagccc
     6121 cgacacgccc agcttgccag aggccgtatt tgccagtaaa tcgctctgca cactggtatc
     6181 cattaaggcg tgaatcctgc ccgaagccgg atccatggac acctgggcat ttgataattg
     6241 cacatgaaac actttcaata ctgtcagcgg cttagccagt ttgctattga gcttttgctg
     6301 cagctgcgca gtgctcatgc tcaccttgcg ctcacccatg ctggcgcagc cgggcagcaa
     6361 taacgatacc agcaggataa aaaaagaagt tt
//
LOCUS       DATMSK010000246         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_348954,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000246 DATMSK010000000
VERSION     DATMSK010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348954"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /gene="ppx"
                     /locus_tag="VNZ84_08165"
     CDS             complement(<1..1431)
                     /gene="ppx"
                     /locus_tag="VNZ84_08165"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HWU66970.1"
                     /translation="MLNITHDTQLAAVDLGSNSFRLEIGRVVDGQILRVDYLKEAVRQ
                     GGDLDEDRNLKPQAFERGLKCLARFGERLKGFPAEHVRAVATQTLREAANSEDFIKQA
                     QKVLGYPIEIISGVEEARLIYQGVSRMLPQSDEKRLVIDIGGRSTEFILGQHFKANVT
                     ESLRLGSVGWSQKYFADGQFSERNFERAEIAAESIIDMIASRYIGQSEVAYGASGTVG
                     AVADVLAGAGFAADRIERSQLEWLKQTLIKAKTPDRLNMEGLKDDRRAVIGGGLAILT
                     AVFDTLQIETLMVANGALRHGVLYDMLSSDHATEDIRTVSITRLCKQFGVEMDHANNV
                     SKAALYFYDAMQASDSHKPLLHWAALCHEIGWAISHTDCNKHGAYILDNTELMGFAQS
                     ELHTISLLVLGHQGKLRKLPADFDNQDLVCQLMALRLAVIFCHSRRMPALKHLQLSRH
                     KQQFVLALSAQWANQHPQTHYLLEEEC"
     gene            complement(1450..1722)
                     /locus_tag="VNZ84_08170"
     CDS             complement(1450..1722)
                     /locus_tag="VNZ84_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66971.1"
                     /translation="MATIEVTETSPNSFSVTVHGSHITTHQVSVKPDYAGELVGTSHN
                     VAELVTASFKFLLDRESNTSILRSFDLSVIERYFPEYPKAINRYLK"
     gene            1877..2563
                     /locus_tag="VNZ84_08175"
     CDS             1877..2563
                     /locus_tag="VNZ84_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66972.1"
                     /translation="MRRLLFFILMLWIAQAAHAARPLVTDDARLTNAHSCQLETWMRS
                     YQGGQELWALPACNLGENFEITLGGGSYRNTDDHYHTEDWVGQFKTLFKPLETNGWGI
                     GFAAGRVMHPDIQPGPNQLGNTYFYIPVSFSLRDDDIIVHVNMGMLRDRQADRNKVNM
                     GLGSEFKLAGRFRGIAEVFGDHTQNPFYQVGLRYSLVPEVFQIDGTLGQQVNGNSGTE
                     WLSIGVRWTP"
BASE COUNT          731 a          802 c          838 g          778 t
ORIGIN      
        1 gcactcttcc tccagcaggt agtgcgtttg tggatgttgg ttggcccact gtgctgacag
       61 tgccagtacg aattgttgtt tatggcgcga tagttgcaag tgcttcaagg caggcatgcg
      121 acgcgagtgg cagaaaatca cggccaggcg caatgccatc aactggcaca ccaggtcctg
      181 gttgtcaaaa tctgcaggca acttgcgtaa tttgccctga tggccaagca ctaacaggct
      241 gatggtgtgt agttcgcttt gggcaaaccc catcagctcc gtgttatcca gaatataggc
      301 gccatgcttg ttacagtcgg tgtgcgaaat ggcccagccg atttcatggc acagcgctgc
      361 ccagtgcaac aacggcttgt gtgagtcact ggcctgcatc gcatcgtaaa aatacaaagc
      421 cgccttgctc acattgttgg catgatccat ttccacgcca aactgtttgc acagcctggt
      481 aatcgagacc gtgcggatat cttcagtggc atggtcacta ctgagcatgt catacaatac
      541 gccgtggcgc agggcgccgt tggccaccat caatgtctca atttgcaggg tgtcgaatac
      601 ggccgtgagg atggcaaggc cgccaccaat caccgcgcgc ctgtcgtcct tcaggccttc
      661 catattcagg cggtcaggag ttttggcttt gatcagggtt tgtttaagcc attccagttg
      721 tgagcgctca atgcggtctg cagcaaagcc tgccccagcc aacacatcgg caactgcgcc
      781 caccgtacca gaggcaccat aagcgacttc tgattgacca atgtagcggc tggcaatcat
      841 gtcgatgata gactcggcgg cgatttcggc acgctcgaaa ttgcgctcgc tgaattggcc
      901 atcggcaaaa tatttttgtg accaaccgac tgagcccaag cgcaaggatt ctgtcacatt
      961 ggccttgaag tgctggccca ggataaactc ggtagagcgg ccaccaatat caatcaccaa
     1021 ccgtttttca tccgattgtg gcaacatgcg gctcacgccc tggtaaatca ggcgtgcttc
     1081 ttcaacgcca gaaataatct caatcgggta gcccaggact ttttgcgcct gtttgataaa
     1141 gtcttcgcta ttggctgcct cacgcaaggt ttgtgtcgcc acggcacgca cgtgctctgc
     1201 cggaaaccct ttcaggcgct cgccaaaacg cgccaggcat ttcagtccac gttcaaacgc
     1261 ctgtggtttc aggttgcggt cttcgtcgag gtcgccgcct tggcgtaccg cctctttcag
     1321 gtagtcgacc cgtaaaatct ggccatcgac gacacgcccg atttccaggc gaaaactgtt
     1381 ggaacccaaa tccacggcgg caagttgggt gtcatgagta atatttagca tgcgataata
     1441 cgaggtgggt tacttaaggt agcggttaat ggccttgggg tattctggaa aataacgctc
     1501 aatcacgctc aaatcaaaac ttctcaaaat actggtgttt gactcgcggt cgagcagaaa
     1561 cttgaacgat gcggtgacca gctctgcaac attgtgtgag gtgcccacta actcgccggc
     1621 ataatcaggt ttgacgctga cctggtgtgt ggtgatgtgc gacccatgca cggtgacgga
     1681 aaacgagttt ggactggtct cagtcacctc aatagttgcc atgattgcct ccccagacac
     1741 atcgtgcaaa cagtataaat tgttatgttg gcagaggcca gtgaaactaa tatgaaaatg
     1801 aatgcgtgaa tgccggcgat ttcatcaaac cgtcatgtcc ccaaaataag atcaagaaaa
     1861 accgaaatac cacaagatgc gtcgcttact ttttttcata ttgatgctct ggatagccca
     1921 ggctgcgcat gctgcacggc cactcgtaac cgatgatgcc cgcctgacca atgcccacag
     1981 ttgccagctt gaaacctgga tgcgcagtta ccagggcggg caggagttat gggccttgcc
     2041 ggcctgtaac ctgggtgaga attttgaaat cacgctcggt ggcggcagtt accgtaatac
     2101 ggatgatcac tatcacaccg aagactgggt aggccaattc aaaaccctgt tcaagccact
     2161 ggaaaccaat ggctggggca tcggatttgc ggccgggcgc gtcatgcatc cggatattca
     2221 gcctggcccc aaccagctgg gcaacactta tttttatatc cccgtatcat tctcgctgcg
     2281 cgatgatgac atcatcgtac atgtgaatat gggcatgctg cgcgataggc aggccgaccg
     2341 taacaaagtc aatatggggc ttgggtctga gttcaaactg gcaggacgat tccggggcat
     2401 tgccgaagtg tttggtgatc atacccaaaa tcctttttat caagtgggat tgcgttattc
     2461 gctggtgccg gaagtgtttc agattgatgg gactttgggg cagcaggtga atgggaatag
     2521 cgggacggag tggttgtcga ttggggtgag gtggacacct taggttttta tttttccaaa
     2581 aggatttatt tcgcttttat agtggtaggg tgggcatgta tgcccacgtg gatgattgat
     2641 tgctgtttgg gatatttctt ttcgcctata ggcgagttac ttttctttgc ttgcccaaag
     2701 aacaagtaac caaaagaaag tgcaccccag cttccgcttt tatcctgtgc tactcggctt
     2761 gccgggcgtc catcgaaact cgccctgaca agccacatac ttcgtggctt gttgcggaac
     2821 tcagacagcc gatggccgaa cactcccgcc aaccctgcgt tgctcggcgc ggcagatggg
     2881 gaccccgaac accaggtgaa tgccaccatt ggtgctctta acaaaccaat ctgttttttg
     2941 aaccatccat acttctgcga tgtgagtttg gcgagataaa tcctattcca tcacgctgtg
     3001 agtagcggag acaaaataag agtagaggga gcgactgttc gaatcccgca gcagctcaca
     3061 ttttgtgtga gctgccaggt tgagtttcgt gaccgtctta ttttgttgag caacgcaagg
     3121 tagccgcagg ccgtgatggc tgggtgtct
//
LOCUS       DATMSK010000247         9683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_159903,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000247 DATMSK010000000
VERSION     DATMSK010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159903"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..624
                     /locus_tag="VNZ84_08180"
     CDS             145..624
                     /locus_tag="VNZ84_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWU66973.1"
                     /translation="MQVAMNTVVTMTYKLQNSDGDILEESKDPVAYLHGGYDNIFPKV
                     EEAMHGKNVGDVVEVSLDPEDAFGEYDDGLVQIEPASAFPTQDLKVGMQFEGEDETGD
                     VILYTITDIADGKVVVDGNHPWAGQRLLFTATVSSVRSANQEEIAHQHVHGAGGHHH"
     gene            complement(640..1293)
                     /locus_tag="VNZ84_08185"
     CDS             complement(640..1293)
                     /locus_tag="VNZ84_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU66974.1"
                     /translation="MLTVDIQLHQPYGVNSRLDLQARFDLRPGDCLGVMGASGSGKTT
                     LVHAIAGLLSPSAGLIRSEEQVWFDAKQGIQLPPWQRSVGLVFQDGRLFPHMTVEQNL
                     RYGMPEAEGQITLMEVVNVLEIGTLLQRKPGQLSGGERQRVAIGRALLRQPVVLMMDE
                     PLSGVDDHLKQQVLPYIKQVIASFGLPTLYVSHVREEVAEIANEVWVLAQGVLRQPV"
     gene            complement(1293..1982)
                     /gene="modB"
                     /locus_tag="VNZ84_08190"
     CDS             complement(1293..1982)
                     /gene="modB"
                     /locus_tag="VNZ84_08190"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HWU66975.1"
                     /translation="MWALSPAELAVVLLSLKVAACCVLVLLLPAIAMAWLLARKQFWG
                     YAVLDALVHLPMVLPPVVLGYMLLIMLGRRGWVGSWLYEQFHLQLTFHWWGAVIASAV
                     MAFPLMVRAIRQALLQVNPLLEQAAQTLGASPVRTFFTITLPLSYHGVLTGGILAFSR
                     SLGEFGATITFAGNLEGETRTLPLAIYSLTQSPEGDAAALRLVILSVLVSVAALLISQ
                     WLERRLLRSQR"
     gene            complement(2004..2759)
                     /gene="modA"
                     /locus_tag="VNZ84_08195"
     CDS             complement(2004..2759)
                     /gene="modA"
                     /locus_tag="VNZ84_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWU66976.1"
                     /translation="MKKWVWLGLCLFSVYAQAEDKKIRVFAAASLTDVMNVLVKQYSQ
                     QAGVQVLPVYGGSGTLAKQLEQGAPGDLFISADEKWMQYLQDKQLVQVSTIKPWLGNR
                     LVMITPQNKPLTIKAEGATDIDQQALGYWCTGDTLSVPVGIYAKQSLTSLGWWEKLKK
                     RLVSTQDVRATLSLVERDECDVGIVYASDAQASHKVKVAGEFAESSHVPIVYPMALLP
                     QATAETNAFYRYLQSAETRKLLISFGFKPLFND"
     gene            complement(2862..3209)
                     /locus_tag="VNZ84_08200"
     CDS             complement(2862..3209)
                     /locus_tag="VNZ84_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU66977.1"
                     /translation="MQASPLKIRINHEGNIALGPGKIELLQHIEQHGSISAAAKQMRM
                     SYRRAWELVDMMNRCFDQPVVVSTTGGSHGGGAQVTEFGQALMLAYQQILQKSAKAAA
                     DELHLITQHLKKD"
     gene            complement(3218..3766)
                     /gene="scpB"
                     /locus_tag="VNZ84_08205"
     CDS             complement(3218..3766)
                     /gene="scpB"
                     /locus_tag="VNZ84_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWU66978.1"
                     /translation="MREPDNIEEMKRILEAALLTSPQPLALDDLLKLFAGRMERATLR
                     MLLDELKTDWTERTLELVQVASGYRFQAKAEYSEYLSRLNPERQPKYSRAVMETLAII
                     AYRQPVTRGDIEQIRGVAVNPNVIRQLQERDWIDVVGQREVPGRPSLYATTKHFLDDL
                     NLRSLAELPPFEAFAQTEIPLD"
     gene            complement(3778..4563)
                     /locus_tag="VNZ84_08210"
     CDS             complement(3778..4563)
                     /locus_tag="VNZ84_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HWU66979.1"
                     /translation="MDNAVNLQVKIKGETLTELPQDLYIPPDALEVYLETFEGPLDLL
                     LYLIRKHNLDILDIPMANLTVQYMAYVDKMTAVKLELAAEYLLMSAMLIEIKSRMLLP
                     KPEEIAEEDDPRAELVRRLMEYEAIKLAAQRLDAMPKVGEEIDVAAAYYQHITQVKPP
                     EVGLDDLVEAWRNVLKRAKLNQHHKLGRAELSVREHMSLLLRRLSAVQVLRFDELFDL
                     ETEGIPKLVVNFLAMLELAKEGLVRITQQEAFGTIYVQANSST"
     gene            complement(4601..5035)
                     /gene="ybeY"
                     /locus_tag="VNZ84_08215"
     CDS             complement(4601..5035)
                     /gene="ybeY"
                     /locus_tag="VNZ84_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWU66980.1"
                     /translation="MPQLDFSLQLASQAADIPSIAQFKRWVHAALRVETSLTIRVVDE
                     DEGRQLNASYRGKDYATNVLTFPLTEEPLLMGDIIICAPVVAREAIEQGKSLLAHYAH
                     MTVHGVLHLHGYDHEVDAQADLMEAMEIAIMHKLGFSNPYAE"
     gene            5100..5882
                     /gene="tpiA"
                     /locus_tag="VNZ84_08220"
     CDS             5100..5882
                     /gene="tpiA"
                     /locus_tag="VNZ84_08220"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWU66981.1"
                     /translation="MTNRSKLVVANRKMHGNLPDNQQFMQTLLQQTHHHKARYAVCPP
                     HPYLFQAQQVLQNSHITWGGQNMSRYERGAYTGSVSPLMLKEFGCTYAIIGHSERRQR
                     GHDSDETCGERFEAALKAGLTPILCIGETLEEYEQGETDLVVVRQLNPVIAHVGIQAL
                     SKGVIAYEPVWAIGTGKAATPQHAQAILSFIRGHIELLDTQAAQDITLLYGGSMNPSN
                     AAQLLSMPDIDGGLIGGASLIAEDFIRICQIANELTPLKTAA"
     gene            complement(6008..6220)
                     /locus_tag="VNZ84_08225"
     CDS             complement(6008..6220)
                     /locus_tag="VNZ84_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66982.1"
                     /translation="MTRSALLAALLALALTACGEKPAEEAPAAEAPAAEAPAAEAAPA
                     EEAPAAEAAPAEEAPAAEAAPAEEAK"
     gene            complement(6425..7588)
                     /locus_tag="VNZ84_08230"
     CDS             complement(6425..7588)
                     /locus_tag="VNZ84_08230"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU66983.1"
                     /translation="MQQTLTLLNGLTPAQFMAEYWQKKPLLIRGAMPDFNGLLDPSEL
                     AGLACDAEVPSRLIQFDPARQQEGDGWQVMQGPFDDEDFAALPAQHWTLLVQGVNHVL
                     PEAQALLAQFNFIPNARLDDVMVSYAPTGGGVGAHIDSYDVFLLQGAGKRRWRISQQT
                     DLRLIPGAPLRILSHFECEQEWVLEPGDMLYLPPNVAHWGISEDDDCMTYSIGFRAPA
                     ARELAIEFLDACRQEGVPDELYRDPDLQPVADSAEIDAVMVVNVAEMMRRLPWSPAQL
                     GPFLAKYFSEPKAYVVFSPNRKLSMEKFSLRVTQSGVVMDLQTQMLHWQGQYFINGDM
                     VTAPDEAAPLLNLLANQRQLTVSDCQGEAMRNLMPWLHDVYLSGYIHFPEKTA"
     gene            complement(7596..8306)
                     /locus_tag="VNZ84_08235"
     CDS             complement(7596..8306)
                     /locus_tag="VNZ84_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU66984.1"
                     /translation="MLLLAGCSTAPSGGYVDSPNGHPVPVRQDRNTFHQLVKTDLDRL
                     ADIEYAENVSTLRLLMLKMYKRNPAEAQKSGLGSPEQILAYVFEQVAVHHWQFDALGG
                     AQDTAAIKLALNPQYSGDRVLALVVGMQSMLFRAHGNKSAFFLTDSIEPQNLYNAARN
                     IEIAVWKLANARNAKGELLLLTNSQPGEVQNLSFEREFSKLIARTDLLALTLAEKSQR
                     FISRLTQSISTAPFLPFR"
     gene            complement(8330..9628)
                     /gene="phoR"
                     /locus_tag="VNZ84_08240"
     CDS             complement(8330..9628)
                     /gene="phoR"
                     /locus_tag="VNZ84_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HWU66985.1"
                     /translation="MQDIRWKAFWSLLFWAFCCLMVGWFTRAETAWLLFTVGALVYFA
                     AHIYWIHRLYQWALKPSLNFIPNGKGVWEDIFANLYHEMRRHSRSQSQLSSTLERLRH
                     ATSALPDGVVVLNNRNEIEWFNEPAIQRLGLKRQHDENQPIYYLLRQAEFVEYLQAED
                     YSEPLKLKSWRSPDVTLELQIIPFASKQRLLICRDVSAIEKTEVMRRDFIANVSHELR
                     TPLTVIGGFLETIGDMQGEISPDIQPYFDMMQSQTTRMRRIIEDLLTLSRLENNVTLK
                     DEADIDMPALLNQLKKDAQALSQGQHTVTLHMETEVNIRGSYNELLSAMSNLTSNAVR
                     YTPEQGKIDIYWQMRGEDAVFTVQDTGIGIESQHLPRLTERFYRVDSGRSRDTGGTGL
                     GLSIVKHILHHHQASLQVESEPGEGSRFQIIFPAQRVLPV"
BASE COUNT         2547 a         2662 c         2226 g         2248 t
ORIGIN      
        1 tgggcttatg gcaagagaca aacgccatgc ctccatggca gtggcggaag ttacacgggc
       61 aacattatcg ccaccaagaa tgacaccaca tctacaatat cctttagaat attggtttat
      121 taaagaactt taggacctta atacatgcaa gtagcgatga ataccgtggt caccatgacc
      181 tataaactgc aaaatagcga tggcgacatc ctggaagaaa gcaaggatcc ggtggcttat
      241 ctgcatggcg ggtatgacaa tatcttccct aaagtggaag aagccatgca cggtaaaaat
      301 gtgggtgatg tcgtcgaagt cagcctggac ccggaagatg cgtttggcga atatgatgac
      361 ggcctggtac aaatcgagcc ggccagcgct ttcccaactc aagaccttaa agtcggcatg
      421 cagtttgaag gtgaagacga aacgggcgac gtgattttgt acaccatcac cgacattgca
      481 gatggcaaag tggtcgtcga tggtaaccat ccatgggctg gccaacgctt gctgtttaca
      541 gcgacggttt ccagcgtacg ctcagcgaat caggaagaaa ttgcgcacca gcacgtgcat
      601 ggtgcaggtg gccatcacca ctaatcagcc acaagctctt taaacgggtt ggcgcaacac
      661 gccttgcgcc aacacccaca cctcattggc gatttcagcc acttcttctc gcacatgact
      721 gacatataac gtcggcaacc cgaatgacgc aatcacctgc ttgatataag gtaatacctg
      781 ctgcttgaga tggtcatcca ctccgctcag gggctcatcc atcattaata ccaccggctg
      841 acgtaacaaa gcacgaccaa tcgctacccg ctgccgctca cctccagaca gctgccctgg
      901 cttacgctgg agcaaagtac caatttccag cacgtttaca acttccatca gtgtgatctg
      961 accttcagcc tccggcatcc catagcgcag attctgttct actgtcatgt gtggaaacag
     1021 gcggccatcc tggaatacca aaccaacgga ccgctgccaa ggtggcaatt gaatgccttg
     1081 tttcgcatca aaccatacct gttcctcact gcgtatcaag ccggcacttg gggacaataa
     1141 accggcgatc gcatgcacca acgtggtttt gccagaaccg gaggcgccca tcacgcccag
     1201 gcaatcgcca ggacgcaaat caaacctggc ttgcaggtct aaacggctat ttacgccgta
     1261 gggctgatgc agttgaatat ctaccgtcaa cattagcgct ggctcctgag caggcgacgc
     1321 tccagccatt ggctgatgag caaggctgct actgacacta gcacactgag tatgactagg
     1381 cgtaaagctg cggcatcgcc ttctggcgat tgcgtcaggc tatagatcgc taacggcaat
     1441 gtacgggttt ccccttcaag attaccggca aaagtaatag tagcgccaaa ctcgcccagg
     1501 ctacggctaa acgccagaat tccgccagtg agcacgccat gataggagag gggcaaggta
     1561 atggtaaaaa atgtgcggac tggggaagcg cccaaggtct gcgcagcctg ctcaagtaaa
     1621 ggattgacct gcaatagcgc ctgacggatg gccctcacca taagcggaaa tgccatgaca
     1681 gcagaagcaa tcaccgcccc ccaccagtga aaagtgagtt gcagatgaaa ctgctcataa
     1741 agccagctac ccacccagcc acgccggccc agcatgatca gcaacatata acccaacact
     1801 acgggtggga gcaccatggg caaatgcacc agcgcatcca atacggcata tccccaaaat
     1861 tgctttctag ccaacaacca ggccatggca atggctggca acaacaatac cagcacacag
     1921 caggcggcca ccttcagtga taacaataca actgccagct ctgccggaga aagtgcccac
     1981 ataatcaaaa caagtgctca gggttaatcg ttaaataatg gtttaaaacc gaaactgatc
     2041 agcaactttc gcgtctcggc ggattgcagg taccggtaaa aggcattggt ttccgccgtc
     2101 gcctgcggca acaatgccat cgggtaaaca atcggcacat ggctgctttc ggcaaactcc
     2161 ccggcgacct ttaccttatg agacgcttgc gcatcactgg cataaacaat gcctacgtcg
     2221 cactcatcgc gctcaaccag gcttaaggtt gccctgacat cttgcgtact caccagccgt
     2281 tttttgagct tttcccacca gcccaaacta gtcagggatt gtttggcata aatccctact
     2341 ggcaccgata acgtatcccc agtgcaccaa tagcccagtg cctgctggtc aatatctgtg
     2401 gccccttccg ccttgatcgt aagcggtttg ttctgaggtg taatcatcac caaccggtta
     2461 cccagccatg gcttgatagt agacacctgc accagctgct tatcttgtag gtattgcatc
     2521 cacttttcat ctgccgaaat aaacagatct cctggagccc cttgttccag ctgcttagcc
     2581 aaggtaccgg aacccccgta tacaggcagc acttgcacac cagcctgctg gctatattgt
     2641 ttcaccagca cattcatgac atcggtcagg ctggccgcag caaataccct gattttttta
     2701 tcttcggctt gcgcataaac actgaacaag cacaaaccca gccataccca ttttttcatt
     2761 cgctccaccc tgctcaatta aaagccacca ctgcgaaaac cattcgcagt cacgatgcta
     2821 ccatcgttat acccacatat acattacgat gcctaaaaaa attaatcttt tttcaaatgc
     2881 tgcgtgatta aatgcaattc atcagcggca gcttttgccg atttctgcaa aatttgttga
     2941 taggccagca tcaaggcctg gccaaattca gtcacctgcg caccacctcc atgcgagcca
     3001 cccgtggtac tcaccaccac cggctggtca aagcaacggt tcatcatgtc taccagttcc
     3061 caggcgcggc gataagacat gcgcatttgc ttggcagctg ctgaaatcga gccatgttgc
     3121 tcgatgtgct gtaacagctc tatcttgcct ggaccgagcg cgatattgcc ttcgtgattg
     3181 attctgattt taagtgggga ggcttgcatg gtgtttgtca atccagcggg atttctgttt
     3241 gcgcaaaggc ttcaaagggc ggtaactctg ccaacgaacg caaattcagg tcatccagaa
     3301 aatgcttggt cgtcgcatac agtgacggcc ggcctggtac ttcacgttgg ccgaccacat
     3361 caatccagtc gcgctcctgc aattggcgga tcacattagg attcactgcg acgccacgga
     3421 tttgctcaat atctcctcgc gtgactggtt ggcggtacgc aataatcgcc agcgtctcca
     3481 tcactgcccg tgaatatttt ggttggcgct caggattcaa acgtgagagg tactcgctat
     3541 actcagcctt ggcctggaag cgatatccgc tggcgacttg caccaactcc agcgttctct
     3601 ctgtccagtc ggttttcaat tcatccagta gcatgcgcaa tgtggctcgc tccatacgcc
     3661 cggcaaataa cttgagcaaa tcatccagcg ccaaaggctg aggcgaggtc agcaaggccg
     3721 cctccaggat acgcttcatt tcctcaatat tgtctggttc acgcatggcg ctactttcta
     3781 agttgagcta ttcgcctgca cataaatcgt tccaaacgct tcctgctggg tgatgcgtac
     3841 caagccttct ttcgcaagct caagcatggc aagaaaattc accaccagct tggggatacc
     3901 ttccgtttcc aggtcaaaca gttcatcaaa acgaagtacc tgtactgcac ttaacctgcg
     3961 cagcagcaaa ctcatgtgct cccgcaccga gagttcggca cggcctaatt tatggtgttg
     4021 attcagcttg gcgcgcttga gcacattccg ccacgcctca accaagtcat ccagccccac
     4081 ctcgggcggt ttgacctggg taatatgctg gtaatatgcc gccgcgacat caatctcttc
     4141 gcccactttg ggcatggcat ccagccgttg tgccgccagc ttgatcgctt catattccat
     4201 caaccgcctc actaattcgg cacgcggatc gtcctcttca gcgatctctt caggcttggg
     4261 taacaacatg cgcgacttga tctcaatcaa catggcggac attagcaaat actccgcagc
     4321 caattcaagt ttcaccgcgg tcatcttgtc cacataagcc atatactgca ccgtgagatt
     4381 ggccatggga atatccagga tatcgagatt gtgcttgcgg atcaggtaaa gcaacaaatc
     4441 cagcggacct tcaaaggttt ccagataaac ttccagtgca tccggcggaa tatacaagtc
     4501 ttgtggcagc tcggtgagcg tttcaccctt gattttgact tgtagattta cggcgttatc
     4561 catacacgga ttgtaacgat ttgccgcaga ggatcaaagg ttattctgca taaggattgc
     4621 taaagcccaa cttgtgcatg atggcaattt ccatggcttc catcaaatcg gcttgcgcat
     4681 ccacctcatg atcataacca tgcaaatgaa gtacgccatg taccgtcata tgggcataat
     4741 gcgccagcaa agatttacct tgctcaatag cctccctggc aactactggc gcacaaatga
     4801 taatatcccc catgaggagc ggctcctccg tcaacggaaa ggtcaacaca ttagtcgcgt
     4861 aatccttacc gcgatagctc gcattcagct gccttccctc atcttcatcg acaacacgga
     4921 ttgtcaggct ggtttctaca cgcaaggcgg catgcaccca acgtttaaat tgcgctatag
     4981 aaggaatatc ggccgcttgc gaggccaatt gcagggaaaa atcaagttga ggcataccgt
     5041 caagttttct gtaaaatatt cgttattata gaaagaaaac ccacaaagga aacggcgtga
     5101 tgacaaatcg ctccaagctg gtggttgcca accgcaaaat gcacggcaac ttgccagaca
     5161 atcagcagtt catgcaaacg ttattgcaac aaacccacca tcataaggca cgttatgcgg
     5221 tgtgcccgcc acatccttat ctgttccagg cgcaacaagt gctccaaaat agccatatta
     5281 cctggggtgg tcagaacatg agccgttatg aacgaggcgc ttatactggc tcggtgtcac
     5341 cactgatgct caaagagttt ggctgcacat acgcgattat cggtcactct gagcgtaggc
     5401 aacgtggcca cgacagcgat gaaacgtgtg gtgaacgttt tgaagcagcc ttgaaagctg
     5461 ggctaacccc catcttgtgt attggtgaaa cgctggaaga atatgaacag ggtgaaaccg
     5521 atttggttgt cgtgcggcaa ttgaatcccg ttattgcgca tgtcggcatc caggcattgt
     5581 caaaaggcgt gattgcttat gaacccgtct gggccatagg cacgggcaag gccgcaacgc
     5641 ctcaacacgc gcaggccatt ttatcgttta tccgcggcca tattgaattg ctggacacgc
     5701 aagccgcgca ggacattacc ttattatatg gcggtagcat gaatccaagt aacgcggctc
     5761 aattactaag catgcctgat attgatggtg gcttgatcgg cggcgcctcg ctcattgcag
     5821 aggattttat ccgcatttgc cagattgcca acgagttaac cccccttaaa actgcagcct
     5881 aaccttaaca accgaacaac cttgcatgag gttagccaat aaaaaagccg actttcgtcg
     5941 gcttttctac atctaagtaa actagatttt tactaacaac agtaaaacca gattactgta
     6001 gtctggatta cttagcttct tcagcaggag cagcttcagc agcaggtgct tcttcagcag
     6061 gagcagcttc agcagcagga gcctcttcag caggagcagc ttcagcagca ggcgcttcag
     6121 cagcaggagc ctcagcagca ggcgcttctt cagctggttt ttcaccacaa gcagtcaaag
     6181 ccaaagccaa caatgcagcc aacaatgcag aacgtgtcat ctttatttcc ttagtatgta
     6241 tgagcaaaaa attatgtacg gacaatcctc tagatcaaga accagccgag tgaaaaaatt
     6301 gtatcagtaa tttattaaaa aaagaacgca aaaccaacga aaaacaaagg atttttttat
     6361 ctatgcaccg ctggcctgtg tttttttcat caaaaaccac cgaaatcgtt tgcaaccaat
     6421 acaattaagc tgttttttca ggaaaatgta tatagcctga cagatacaca tcatgcaacc
     6481 acggcattaa attacgcatg gcttcaccct ggcaatcaga gacggtcaat tgacgttgat
     6541 tggccagcaa attcagcaat ggtgcggcct catcaggcgc cgtcaccata tcaccattga
     6601 taaaatactg cccttgccaa tgcaacatct gagtctgcaa atccatgacc actccgcttt
     6661 gtgtcactcg taggctaaac ttttccatgc tgagtttacg attgggcgaa aaaaccacat
     6721 atgccttggg ctcagaaaaa tattttgcca aaaaaggacc taactgcgca ggcgaccatg
     6781 gcaaacggcg catcatctcg gctacattga cgaccattac tgcatcgatc tcagcactat
     6841 ctgcaaccgg ttgcaaatca ggatcgcgat acaactcgtc aggaacacct tcttgccggc
     6901 aggcatctaa aaactcaatc gccagttcac gtgcggctgg agcacggaaa ccaatagaat
     6961 aggtcatgca gtcgtcatct tcggagatac cccaatgagc gacattcggc ggcaaataaa
     7021 gcatgtctcc cggttccagc acccactcct gctcacactc gaaatgcgac agaatgcgca
     7081 gtggcgcgcc tggaatcagc cttaaatcag tttgctgact gatgcgccaa cggcgcttgc
     7141 cagcaccttg cagtaaaaaa acatcgtaac tatcaatatg ggcgccaaca ccaccaccgg
     7201 taggcgcata actgaccatc acatcatcca gcctggcatt tggaataaaa ttaaattgtg
     7261 ccaacagggc ctgtgcttcc ggcaacacat gattgacccc ctgcaccagc aaggtccaat
     7321 gctgcgcggg gagtgccgca aagtcttcgt catcaaaggg cccttgcatc acttgccagc
     7381 catctccttc ctgctggcgt gcaggatcaa actgtatcaa cctggaaggg acctccgcgt
     7441 cacatgccaa tccagccaat tcactagggt caagtaagcc attgaagtcc ggcatggcgc
     7501 cgcggatcag cagaggtttc ttttgccagt attcggccat aaattgtgcc ggggtcaaac
     7561 cattaagtaa ggtcagtgtt tgttgcatgc ttaaattatc tgaaagggag aaaaggtgcg
     7621 gtactgatac tttgggtcag gcgggaaata aaacgttgcg acttttctgc cagcgttaac
     7681 gccagtaaat cggtacgtgc aatcagcttg ctaaactcgc gttcaaagct gagattttgc
     7741 acctcacctg gctggctatt ggtcaggagt aataactcac ccttggcatt tctggcatta
     7801 gccaacttcc agaccgcaat ttcaatattc ctggccgcat tatacaaatt ttgcggctct
     7861 atactatccg tcagaaaaaa agctgactta ttcccgtgtg cccggaacag catggactgc
     7921 atccccacaa ccaatgcaag cacacgatcc ccactgtatt gcgggtttaa cgccagttta
     7981 atggcagctg tatcctgggc gccacccaac gcatcaaact gccagtgatg gacagctacc
     8041 tgttcaaaca cgtaagccaa aatttgctct ggcgagccca agccagactt ttgtgcctcg
     8101 gccggattac gtttgtacat tttgagcatg agcagccgca gtgtactgac attctcggca
     8161 tattcgatat ccgccaaacg atcaaggtca gttttgacca actggtggaa ggtgttgcgg
     8221 tcctgccgca ctggaaccgg atggccattc ggcgaatcga cataaccacc actaggcgct
     8281 gtactgcaac cggccagcag taacatcacg acactgcagc cggacaacct catacaggca
     8341 atacccgctg ggcaggaaag ataatctgaa agcggctgcc ctctcctggc tcgctctcaa
     8401 cctgcaaact cgcctgatga tgatgcaaaa tgtgcttcac gatggaaagg ccgagccctg
     8461 tgccgcctgt atcccggcta cgtccgctat ctacacgata aaagcgctca gtcagacgag
     8521 gtaaatgctg cgactctata ccgatgccgg tatcctgcac agtaaacact gcatcttccc
     8581 cgcgcatctg ccaatagata tcaatcttgc cttgctcagg cgtgtaacgc acagcattgc
     8641 tggtcaggtt gctcatggcg ctcaataact cattgtagct gccgcggata ttcacttcgg
     8701 tttccatgtg caaggtcacg gtatgctgcc cctggctcaa ggcttgcgca tcttttttta
     8761 actgattcag tagtgcaggc atgtcaatat ccgcctcatc tttgagcgtg acattatttt
     8821 ccaggcgcga taacgtgagc aagtcttcga tgatacggcg catgcgtgtg gtctgtgact
     8881 gcatcatgtc aaaataaggc tggatatccg gcgaaatttc gccttgcata tcgcctatgg
     8941 tttcgaggaa gccaccaatg acggtgagtg gcgtacgcag ctcatgtgac acattcgcaa
     9001 taaagtcgcg gcgcatgact tcggtctttt caattgcgga cacatcacga caaatcagta
     9061 aacgctgctt gctcgcaaac gggatgatct gcagctctag tgtcacatcc gggctgcgcc
     9121 aggacttaag cttgagcggc tcgctataat cctcggcctg caaatactcc acaaattcag
     9181 cctgccgcag caaatagtag atcggctgat tttcatcatg ctgccgcttc aagcccagcc
     9241 gctggatagc aggctcatta aaccattcaa tctcattgcg gttattcagt accacaaccc
     9301 cgtcaggcaa ggcactggtc gcatggcgta gacgctccaa tgtgctgcta agctggcttt
     9361 ggctgcggga gtggcgacgc atttcatgat agaggttagc aaaaatatct tcccagacgc
     9421 ctttaccgtt gggaataaaa ttgaggctgg gtttgagtgc ccattggtac aagcgatgaa
     9481 tccagtagat gtgtgctgca aaataaacca gcgcaccaac cgtaaacaac aaccaggcgg
     9541 tttctgcacg ggtaaaccag ccaaccatca ggcagcaaaa cgcccaaaac agcagactcc
     9601 agaatgcttt ccaacgaata tcttgcacga gtcattctca acacatcgat ggcagcgatt
     9661 atacgacaat ccggtttgcc cgc
//
LOCUS       DATMSK010000248         3507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_302760,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000248 DATMSK010000000
VERSION     DATMSK010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302760"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..2222)
                     /locus_tag="VNZ84_08245"
     CDS             complement(108..2222)
                     /locus_tag="VNZ84_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWU66986.1"
                     /translation="MFIRKTLPYAVLSACAFAQPGMAAETTDTGHEERISILAPIVVT
                     ATRQAQDSFDLPVAIDVVEKENIQDGQARMTLSESLIRVPGITAQNRTQMAQDPQIST
                     RGFGARSSFGVRGVRVYVDGIPLTMPDGQGQPGNVDLDIVQGVEVLRGPFSALYGNSS
                     GGVIQMLTEDAAKTPEVSAGVVFGSYGTSKESVRASGTSEGIEYLVNYSNYESDGYRD
                     HSASSKKQGTAKFKINLNEDTKLTTLVNWFDQDAQDPGGLTRNASATEPSAFNDPKSV
                     SRATLRSNTRVSRSNTQVGFNLEHTINDNNSINFINYVGTRENLQYLSLNSTGGGRAS
                     AISREFWGSELRWNNQGFLLSKPYTLSAGLVYGKMNDDRLDTPTTNGEKSAAATRNEK
                     NIASNSDQYLQGKWSVLDNLDLHAGLRRTKVSLEIDDKFITNTNPDNSGSLTYEKTTP
                     VIGAVWKVNPLLNFYANYGKGFETPTFIEVAYLNSNINSTPNFGLKPSTSKNFEIGTK
                     AFLSDNTLLNATIFKTLTDDEIIALTTGTFATYTNAAKTKRNGAEVSIESKLAHNISL
                     YGAYTLLNAKFDSDYVGNLGLVNSGNTIPGTYHTQIYAEAAWKHIESGFRTAFEARHN
                     SKAYVNDTNTDSAPSYTIFNVRAGFEQQLKSWRFSEFVRVENIFDKDYIGSVRVNDGN
                     SRFFEPAAGRNWLLGLNANYRF"
     gene            2408..2791
                     /locus_tag="VNZ84_08250"
     CDS             2408..2791
                     /locus_tag="VNZ84_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU66987.1"
                     /translation="MAVTGFNHYNLRATREQMAVLLDFYTRVVGLTLGERPGLSSFGY
                     WLYAGAKDVLHLSEVKEGVEPALNVQTTFDHVAFTCTDYAAMEQHLQAHGVQFGSRVV
                     KATNVRQIFFKDPFGNGVEFNFDES"
     gene            complement(2833..>3507)
                     /locus_tag="VNZ84_08255"
     CDS             complement(2833..>3507)
                     /locus_tag="VNZ84_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HWU66988.1"
                     /translation="TGDELVSVGQALQAGQIYDSNRYTLHGMLTRMGVEIMDLGAIKD
                     DPQVLEQVLTKAAAEADVVITSGGVSVGEADYMKQLLQKLGSVLFWKIAMKPGRPLAY
                     GKIGSAHYFGLPGNPVAVMVTFYQFVREAMLVLMGQAAPPPLPMLQAICVEEIRKAKG
                     RTEFQRGILFADADGTWKVKTTGAQGSGMLSSMSRANCFIVLDEDTGSLPANQLVRVQ
                     LLEGII"
BASE COUNT          752 a          868 c          976 g          911 t
ORIGIN      
        1 caaggcatca ctatacgatg atagagatgc cttgtagctt agccgacttt ggcagatggc
       61 gtctgtgact gaagttactt aagccagccc agtcgtttta ccgtctatta aaaacgataa
      121 ttggcgttca gacccaacag ccagttacgg ccagctgccg gttcaaagaa gcggctgttg
      181 ccatcattta cccgtacaga accaatgtaa tccttgtcga aaatgttttc aacacggaca
      241 aactcgctga agcgccagct cttgagctgt tgctcaaaac ctgcgcgtac attgaagatg
      301 gtatacgacg gtgcgctatc ggtattggta tcgttgacgt acgccttgct gttgtgacga
      361 gcttcgaatg cggtgcggaa accggactcg atatgcttcc aggcggcttc ggcgtatatc
      421 tgcgtgtggt aagtgccagg aatggtattg cctgaattga ccagtccgag attgccgaca
      481 taatccgaat caaatttcgc attcaacagc gtataggcgc catacagcga aatgttgtgt
      541 gcgagcttgg actcaatgga aacttccgca ccattgcgtt ttgtttttgc cgcattggtg
      601 taagttgcaa atgtgccggt ggtcagcgca atgatttcgt cgtcggtcaa tgtcttgaag
      661 atagtggcat tcagcagtgt attgtctgac aggaaggcct tggtcccgat ttcgaagttc
      721 ttgctggtac tgggcttgag gccgaagttg ggggtgctgt taatgttgct gttcaggtag
      781 gccacttcga taaaggtagg tgtttcaaaa cctttgccat aattggcgta gaaattgagc
      841 agtgggttta ctttccagac agcgccaatc actggggtgg ttttttcgta ggtcaggcta
      901 ccgctattgt ctggattggt attggtgatg aatttgtcat caatctccag ggatactttg
      961 gttctgcgca gaccagcatg caggtcaagg ttatcgagca cagaccattt gccttgcaga
     1021 tattggtcgg agttggacgc gatgtttttt tcattacgcg tggccgctgc acttttctcg
     1081 ccattggtcg tcggcgtgtc cagacggtca tcattcattt ttccgtatac caggcctgcg
     1141 ctcaatgtgt acggcttgct aagcaagaag ccctgattgt tccagcgcaa ttcgctgccc
     1201 cagaactctc tggagatggc actggctcga ccgcctccgg tactgttcag cgaaaggtac
     1261 tgcaggtttt cccgtgtacc gacatagttg atgaagttaa tggagttatt gtcattgatc
     1321 gtatgctcaa ggttaaagcc gacctgagtg ttactgcgag agacgcgtgt gttagagcgc
     1381 agcgttgctc tgcttacact cttggggtcg ttaaatgctg aaggctcggt ggccgaggca
     1441 ttgcgcgtta agccacctgg atcctgtgca tcctgatcaa accagttaac cagtgtagtg
     1501 agcttggtgt cttcattgag gttgatcttg aatttggcgg tgccttgttt tttgctgctg
     1561 gcgctatggt cgcgatagcc atcagattcg taattggaat agttcaccag atactcaatg
     1621 ccttccgaag taccagatgc ccgcacgctt tccttgctgg tgccataact gccaaacacc
     1681 acgcctgcag aaacttcagg tgttttggcc gcgtcttctg tcagcatctg gatcacgcca
     1741 ccggaggagt tgccatacag ggcggagaat gggccgcgta acacttctac gccctggaca
     1801 atatcaagat caacattgcc tggctgaccc tgtccatcgg gcatggtcag cggaatgcca
     1861 tcaacgtaca cacgcacgcc acgcacacca aacgatgagc gggcgccaaa gccacgagtg
     1921 gagatttgcg ggtcttgcgc catctgcgtg cggttttgcg cggtgatgcc ggggacgcgg
     1981 atcaggcttt ctgacagggt catgcgggcc tgaccgtcct gtatgttttc tttttcaacg
     2041 acatcaatcg ccaccggcag gtcaaaacta tcctgcgctt gacgggtggc ggtgaccacg
     2101 atgggggcta ggatggaaat gcgttcttcg tgaccggtat cggtggtttc agcggccatg
     2161 ccgggttggg caaaggcgca tgcgctcaaa acggcgtatg gcagtgtttt tcggatgaac
     2221 atgatgctcc cttgagaagt taatttttgt aatgggcgga gcatagtaca ttcgggggtt
     2281 tgagcgcatc aggataatca tgaatcttca ttatgattcg aggcaatagt gcatacttta
     2341 tgggtaaagc aggcggtgcc cctggaggtg tagaatattc agcctgacct accaaaaagg
     2401 acatcttgtg gccgttaccg gatttaatca ttacaacttg cgggcgacgc gcgagcagat
     2461 ggcagtgttg ctcgactttt acaccagggt ggtgggcttg accctgggcg agcgtcctgg
     2521 gctcagcagc tttggctact ggttatatgc cggtgccaaa gacgtgctgc atttgtcgga
     2581 agtgaaagag ggtgtggaac ccgcgctgaa tgtgcaaacc acgtttgatc atgtggcttt
     2641 tacctgcacg gattatgccg ccatggagca acatcttcag gcccatggcg ttcagtttgg
     2701 cagccgcgtc gtcaaggcga ccaatgtcag gcagattttt ttcaaggatc cgtttggtaa
     2761 cggcgttgag tttaatttcg acgagagtta aggccgggct ggcccttgga taggccagcc
     2821 tggtagtgaa tattaaataa tgccttccag caactgcact ctgaccagtt gattcgcggg
     2881 caggctgccc gtgtcttcat ccagcacaat aaagcaattg gcgcgcgaca tggagctcag
     2941 catgcctgag ccttgcgcgc ctgtggtttt gactttccag gtaccatctg catcggcaaa
     3001 taaaataccg cgctgaaact cggtgcggcc tttggctttg cgtatctctt ccacacaaat
     3061 ggcctgcagc atgggcaatg gcggcggcgc tgcttgcccc atcagcacca gcatggcttc
     3121 gcgcacaaac tggtaaaagg tcaccatgac agcgaccgga ttgccaggca agccgaaata
     3181 atgcgcgcta ccaattttgc cgtacgccag cgggcggccg ggtttcatgg cgattttcca
     3241 gaacagcacg gagcccagct tttgcagcaa ttgtttcatg taatcggctt cccccacgga
     3301 gacgccgccg ctggtaatga ctacatctgc ctcagccgcg gctttggtca gcacctgctc
     3361 cagtacctgc gggtcatcct tgattgcgcc caagtccatg atctcgaccc ccatgcgggt
     3421 taacatgccg tgcagggtgt agcgattgct gtcgtatatt tgtcctgcct gcaaggcttg
     3481 gccgacactg accagctcat cgccggt
//
LOCUS       DATMSK010000249         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_160313,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000249 DATMSK010000000
VERSION     DATMSK010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160313"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            686..1195
                     /locus_tag="VNZ84_08260"
     CDS             686..1195
                     /locus_tag="VNZ84_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWU66989.1"
                     /translation="MYSDLIHLGGADKYVDEVLEIINSIKLLDDFSADEVRYLCAYMQ
                     CYAAPRDYSLLVEGTGGDYLLLILSGGVDVYKNDASAEGRHMVARLGIGDTIGEMSLI
                     DGYPRSASCITTHPTDFAVFSRSSIQELLVNMPKLGNKILIKLLQLMTERLRDTSNRY
                     PDGAFFSLV"
     gene            1216..2463
                     /gene="epsL"
                     /locus_tag="VNZ84_08265"
     CDS             1216..2463
                     /gene="epsL"
                     /locus_tag="VNZ84_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated polysaccharide
                     biosynthesis outer membrane protein EpsL"
                     /protein_id="HWU66990.1"
                     /translation="MYASKKLMPFNRCLMLVNFAVFMSIAGTASADDKDVVNFTLSHA
                     VTYDDNLFKLDRSESPTATFGKSQRWEMINATTYGMSINKPYSLQRFKFDVNQTSYRY
                     QNYNFLNFDGTNYDIQWNWSLTPYLKGNLVFDRQQLPNTFANITSINPLTTNTRTVKS
                     NRFDFDWSPQGNWHLLGGVTDLTVTTDNSSPTQNNIDFSFEQTSARAGIRYDLPSGSN
                     LEYASSTANGKYGQSTLNEVLFTDQGYEEKRQEFKFNWVLSGKSKLNMSATYLDRKND
                     NFSQRDVSGWIGAVNYSWDVTGKTRINFIASTDLFPTQAISYTYVRQNLIAIRPTWSV
                     TSKVLVRGDLTLRERAFEGFAPAPPAEDRHDRTRIASISATWEPRRFVSLGVMLKREV
                     RDSNLINDDYVSNSAMINAGLTF"
     gene            2515..3429
                     /locus_tag="VNZ84_08270"
     CDS             2515..3429
                     /locus_tag="VNZ84_08270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWU66991.1"
                     /translation="MTFMKKAVLAAMVVALAACGKSEEKSSSGQVAAIVDGKEISVLQ
                     LNQAMKNMPNVTADNADAVRKQLLDRLVDQQVVLNKALEEKVERSAEVISSIEAAKRE
                     IITRAYLTNMVSSRIKPSPQQVKAYFDGHPELFSARKVYNLQDIALPPGTAIPDELKA
                     LVERSKGLQEVAEWLKSQNVKFGASSYSRPAEQVPTDALAVLKDQKDGAVLIVPMDGN
                     PHIVKLIGAIVSPMPYDQAKPLIQTFLVNSEGQKLIKSELAKLKEKSKIEYVGAFAKS
                     ADTAVAKPEAKPAEKNNSHIEKGVVGLD"
     gene            3494..4531
                     /locus_tag="VNZ84_08275"
     CDS             3494..4531
                     /locus_tag="VNZ84_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HWU66992.1"
                     /translation="MNIIGTLLLVTAFNAVAAEEYALGAGDVVRIQVFQNPDLTTEAR
                     VSESGEITFPLLGTVNVGGAGIGQAERKIAQGLVQGGFVQKPQVNIVLVQVRGNQVAV
                     LGLVNRPGRFVIETFDTHLSEMLATAGGVLSAAGSGKAIITGQRDGQSFRREVDLAEL
                     FLLDKKEADLLVAKGDVIYIVPGNQVSILGQVNRPGRYALEEAKMKLSDALAMAGGVS
                     VSGSDSAILEGVRDGQKFHKVVDIAAIYMDENSDMNPDVLAGDSIYVHRAPVFYIYGE
                     VQRPGSYRVERNMTIMQALAEGGGPTPRGTQRGLKLHRKNASGVVEKTAPALDELVQS
                     GDVLYVKESLF"
     gene            4541..>5245
                     /locus_tag="VNZ84_08280"
     CDS             4541..>5245
                     /locus_tag="VNZ84_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWU66993.1"
                     /translation="MNLNQIVLILLARYRIVLITFALTVFTVLVISLLQSKVYQATAS
                     VVLNYKGTDPVTGAVVPGNMALGYVPTQIDIINSQHVALKVVDELRMAENENVKAKFE
                     EDTGGRGDLRFWLADLLLKKLTVTPGRDSSVIEIMFEGSDPDFVALVANAFANAYQET
                     NVEMKVEPAQKAAEYFGTQIQALRDNLSKAQSRLSKYQRENGYTSSDERMDVEASRLN
                     WLSEQLVSAQGQSIEAD"
BASE COUNT         1390 a         1168 c         1342 g         1345 t
ORIGIN      
        1 gcccatggag aaatcgcccc aggcggtgat catgatttca tgcggtaaaa actgctgaag
       61 tttgccttgc atccaaagga agaactcgaa gtgcgagcgg attcgcaaag actcgtgcat
      121 caagtcaata agctgtgcct tctcaatatc ggttagttct ttggtaataa acatagccgt
      181 tcaaatataa aaattcattt acgttgcaaa cacttgtctt tgcgagcaat tattgggcca
      241 gctgcaaagg cgccagtact gtccagcagt aaatcctcca ctcaaattgt gggacatgct
      301 gaaaaggata tttgctcatg tgtttgtagg tggccatttt atatttggat caagcgtgga
      361 atcctagcgt attcttgtat gtgacggctg tcactatgat aaaacttttg cctacatcag
      421 tggtagttct tttggactat tgttgtcacc ctctacagtc catacactgc ggtacataat
      481 ggcgctggat cgtagttctg ccggctggaa ggcattcatg tctggtcgtg atcacacttt
      541 ccatggtgac tatggactgt catgcgctga tacctcagcg aatcctttgg tggctatgcc
      601 gatgggcaac cataacaaca ggctgtctac gttcataact tgcttaacgt tagcgtcaca
      661 aatcattgaa agtttttgtt tatccatgta ctctgattta atacatttag gcggggcaga
      721 taagtacgta gatgaagtgc tggaaataat caacagcatc aagctgcttg atgacttcag
      781 cgcagatgaa gttcgctatc tatgtgctta tatgcagtgc tatgcagcgc caagagacta
      841 ttcgttatta gtcgagggta ctggtggcga ctacctgcta ttaatattaa gtgggggggt
      901 agacgtgtat aaaaatgatg catccgcaga gggtcgccat atggtggctc gacttggcat
      961 tggcgatacc atcggggaaa tgtccctgat agacggctat cccagatctg cctcctgtat
     1021 taccacacat cccaccgatt tcgcggtatt ttcacgcagt agcattcaag agctgctggt
     1081 gaatatgccc aagctgggca acaaaatact gataaaacta ttgcagctta tgacggagag
     1141 gttgcgcgac acctctaacc ggtaccctga cggtgctttt tttagtctgg tctaaacacg
     1201 ctgcgagagt taattttgta cgcatccaaa aagttaatgc cgtttaatcg gtgtttgatg
     1261 ttggtgaatt ttgccgtttt tatgtcgatt gcgggaaccg cgtcggcgga tgataaagat
     1321 gtcgtcaatt tcacgttaag tcatgcggta acgtatgacg acaacttatt caaactggac
     1381 cgaagtgaga gtcctaccgc aacgtttggc aaaagccagc gctgggaaat gatcaatgca
     1441 actacctatg gtatgagcat caacaagcca tatagccttc agcgatttaa gtttgatgtc
     1501 aatcagacgt catatcgcta tcagaactat aatttcttga atttcgatgg cacaaattac
     1561 gatatccagt ggaactggtc tttgacccct tacctgaaag gtaacctcgt atttgaccgc
     1621 caacaattgc caaatacgtt tgccaatata acaagtatta accctctcac aaccaatacg
     1681 cggacagtga aaagcaatcg ctttgatttt gattggagcc cacagggcaa ctggcattta
     1741 ttgggtggtg taaccgattt gacggtcaca acggataact cttcgccaac acaaaacaat
     1801 atcgatttca gttttgagca aacctcggcg cgcgctggca ttcgttacga tctgccctca
     1861 ggttcaaatc tggaatacgc atccagtact gccaatggta aatatggtca atccaccctg
     1921 aatgaggtcc tttttacaga tcaggggtac gaggaaaagc ggcaggagtt caaattcaat
     1981 tgggtgcttt cgggcaagtc gaagctgaat atgagcgcta cttatcttga tcgcaagaat
     2041 gacaatttta gtcagcgtga cgtgagtggc tggatcgggg cggtaaacta tagctgggat
     2101 gtgactggca agacccgcat caatttcatt gcgagtaccg atttattccc aacgcaagcc
     2161 atctcgtata cctatgtgcg tcagaatttg atcgctatcc ggccgacatg gtctgtcacc
     2221 tcgaaagtgt tggtcagagg agacttgacg ttgcgtgaac gtgcattcga aggttttgcc
     2281 cctgcacctc ctgccgagga tcgacatgac cgcacaagaa ttgccagtat cagtgcaacc
     2341 tgggaaccgc ggcgtttcgt gagtctggga gtaatgttga agcgcgaagt gagagattca
     2401 aaccttatca acgatgacta tgtctcaaat agcgcgatga taaatgcggg cttgaccttc
     2461 taaaaaataa gccgaccaat gtatggccgc gcatatcgtg aatatggagc aataatgact
     2521 tttatgaaaa aagcggtatt ggccgccatg gtggtcgctt tagccgcctg tggcaaatcg
     2581 gaagaaaaat cttcatctgg acaagttgct gccattgtag atggcaagga aatatcggtc
     2641 ttgcaactta accaagccat gaaaaacatg cctaacgtga cagctgacaa tgccgacgcg
     2701 gtacgcaaac agttgcttga tcgtctggtc gatcagcagg tggttttaaa taaagcgctg
     2761 gaagaaaagg ttgagcgcag tgctgaggtc atttcgtcaa tagaagcggc caagcgcgaa
     2821 attatcacca gagcctattt gactaacatg gtcagttcgc gcatcaaacc aagtccgcaa
     2881 caggtgaagg cctattttga tgggcatcct gagctttttt ccgcccgtaa ggtttataac
     2941 ctgcaagata ttgccctccc gccaggcact gcaattcctg acgagttgaa ggccttggtt
     3001 gaacgcagca agggcttgca agaggtggct gagtggctca aaagtcagaa tgtcaagttt
     3061 ggtgcttcca gctacagcag gccagccgag caagtgccga ccgatgccct tgcggtgctt
     3121 aaggatcaaa aagatggcgc tgtcctgatc gtgcccatgg atggcaatcc gcacattgtg
     3181 aaactgattg gtgcgattgt ctcccctatg ccttatgacc aggcaaagcc tttgatccaa
     3241 accttccttg tgaactcgga aggccaaaaa ttgatcaagt ctgagttggc taaactcaag
     3301 gaaaaatcca agatcgaata tgttggtgct tttgccaaga gtgccgatac cgcggtagca
     3361 aagcctgaag cgaagcctgc agagaaaaac aacagccata tcgaaaaagg ggtagtgggc
     3421 ctggactaag cagcgctcat catcacttaa ctaaaccata atcaaacagg gaaatatcat
     3481 gatgaaatgg tgcatgaata tcatagggac gcttctttta gtcacggcat ttaatgcagt
     3541 ggcagcagaa gagtatgcac ttggtgcagg tgacgtggta cgtatccagg ttttccagaa
     3601 ccctgatctg acgacagaag cgcgggtttc cgagtcaggc gaaatcacat ttcctctgct
     3661 gggaaccgtg aatgttggcg gtgcagggat tggtcaagcg gagcgcaaga ttgcccaagg
     3721 tctggtacaa ggtggttttg tgcaaaagcc gcaagtcaat atcgttctgg ttcaggtgcg
     3781 tggcaatcag gtagccgtgt taggcctggt aaatcgtcct ggtcgttttg tcattgaaac
     3841 cttcgatacc catttgagtg aaatgctggc gaccgcaggt ggcgtgctct cagctgcagg
     3901 tagtggcaag gccatcatta ctggccagcg cgatgggcag tctttccgtc gtgaggttga
     3961 tctggccgaa cttttcttgc tggacaaaaa agaggcagac ttgctcgtgg ccaaaggcga
     4021 tgtcatctac atcgtacctg gcaatcaggt gtctatcctt ggacaagtga atcgtcctgg
     4081 tcgctatgct cttgaagagg ccaagatgaa gttgtcagat gccctcgcca tggccggcgg
     4141 tgtgagtgta agcggatcgg attccgccat tctggaaggt gttcgcgacg gtcagaagtt
     4201 ccacaaggtg gttgatattg ccgcgatata tatggatgaa aactctgaca tgaatcctga
     4261 tgtactggcc ggtgattcca tctatgtgca ccgtgcgccc gtgttctaca tttatggcga
     4321 agtacagcgt ccaggctctt atcgtgttga gcgcaatatg accatcatgc aagcgctggc
     4381 agagggcggt ggtccaacac ctcgtggtac gcagcgtggt ttgaaattac atcgcaaaaa
     4441 tgcatctggc gtcgtcgaaa agacagcacc ggcgttagat gaattagtgc agtctggcga
     4501 cgtactttac gttaaagaaa gcctgtttta ggaagctcgg atgaatctga atcaaatcgt
     4561 actcatatta ctggcacgtt accgtattgt attgattaca tttgccctta cggtatttac
     4621 agtcctggtg atcagcttgc tgcaaagcaa ggtttatcag gcaacagcat ccgttgtgct
     4681 gaattacaaa ggcacagatc cggtgaccgg cgcggtggtg ccaggcaata tggcgctggg
     4741 ttatgtaccc acccagattg acatcatcaa tagccaacac gttgctctca aggtagtcga
     4801 cgaattgcgc atggccgaaa acgaaaacgt caaagccaaa tttgaagagg ataccggagg
     4861 gcgtggtgat ctgcgtttct ggttggcaga cttgctcctg aagaagctca ctgtaacgcc
     4921 aggccgggac agcagtgtga ttgaaatcat gtttgaaggc tccgacccgg actttgtggc
     4981 cttggtcgcc aatgcctttg ccaacgctta tcaggaaacc aatgtcgaga tgaaagttga
     5041 gcctgcacaa aaggcggctg agtattttgg tacacagatt caagcgctgc gggacaacct
     5101 ttccaaggca caatccagac tttccaaata tcagcgtgaa aatggttaca ccagctcgga
     5161 tgaaagaatg gatgtggaag catcacgcct caattggctt tctgagcagt tggtatctgc
     5221 acaaggacag tctattgaag cagat
//
LOCUS       DATMSK010000250         5002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_466945,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000250 DATMSK010000000
VERSION     DATMSK010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466945"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VNZ84_08285"
     CDS             complement(<1..273)
                     /locus_tag="VNZ84_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU66994.1"
                     /translation="MTIPAGMLGIRHVALFIKDLESAIDFYTRIVGMHIEWQPDPDNV
                     YLTNHGDVFALHRVDYKADPLQRLDHIGFVLTDESAVDAWHTHMVNH"
     gene            complement(277..702)
                     /locus_tag="VNZ84_08290"
     CDS             complement(277..702)
                     /locus_tag="VNZ84_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWU66995.1"
                     /translation="MQVQVKWIDGVSFVGESETGHAVVLDGAPENGGRNIGMRPMEML
                     LIGMGACTSFDVVTILKKSRQPIVDCVAEISAERADAIPKVFTKIHVHFVVTGNNLNE
                     SQVARAVSLSAEKYCSASIMLQKACEITHDFEIKPHSVE"
     gene            complement(832..1263)
                     /locus_tag="VNZ84_08295"
     CDS             complement(832..1263)
                     /locus_tag="VNZ84_08295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWU66996.1"
                     /translation="MLMLLTVTGCQAQSNSSKTKEAAQMSANVTELQKIDTVVGEGRE
                     AEIGFNVTVHYTGWLYDANKEDHKGQKFDSSLDRNSPFNFFLGGGQVIQGWDEGVQGM
                     KVGGKRTLIIPSDMGYGARGAGGVIPPNAALVFDVELLGVK"
     gene            complement(1360..2730)
                     /gene="dacB"
                     /locus_tag="VNZ84_08300"
     CDS             complement(1360..2730)
                     /gene="dacB"
                     /locus_tag="VNZ84_08300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HWU66997.1"
                     /translation="MAQAALPSDLQEALSKAQLPAESVAIVVQPVDGGPPLISHNAAK
                     AMNPASVMKLVTTYAALEALTPAYRWKTEIYRNGPVSQGILDGDLMIKGYGDPAFNIS
                     DFWRLLQLVQQQGIHQIRGNLVLDLSAYAPEVSQRPVLDDEPWRAYNANPSALLLNGR
                     NTSFRFVASKVAGKPVVQISQEFELPQIRVINTMQTRKGACNDWRGDVHYSITPQADG
                     ATVVFSGTLPEQCDERYLELSVLKDTQYVFFSFKKLWAQLGGQFNGQLLLAPAIDTTN
                     LVTTWWSSPLDNVVRDINKWSNNVMARQLLLTIALEAGFTPADEVGGTLVLKQVLRQR
                     GLQFPELVLENGSGLSRIERISAEHLAQLLVTAWQRPVMPVFMASMPILGMDGTTKKR
                     LADGASKGMAYLKTGSLEGVSSLAGYVQDSAGKRYVLVVIANHARASAVRTVQDTLLK
                     QLIEGR"
     gene            complement(2769..4085)
                     /gene="bioA"
                     /locus_tag="VNZ84_08305"
     CDS             complement(2769..4085)
                     /gene="bioA"
                     /locus_tag="VNZ84_08305"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HWU66998.1"
                     /translation="MSGNQDLLQRSLRSVWHPCTQMKHHERYPLVPITRGEGAWLFDR
                     DGKGYLDAVSSWWVNLFGHNQPVIKEAIKAQLDQLEHVMLAGFTHEPVVALSEKLSAL
                     TGLGHAFYGSDGASATEIALKMSFHYWRNIGQPLKTKFISLQNSYHGETLGALSVTDV
                     AIFKDTYAPLILQSAQMQSPDSRLAEAGETEEAYALRCAEALEVYVAEHHAILAAFII
                     EPLVQGAAGMAMYHPVYLQRAREICSRYQVHLIADEIAVGFGRTGTLFACEQAGEKVS
                     DIADFICLSKGITGGYLPLSVVLTTDKVYQAFYHDQTVKGFLHSHSYTGNPLACSAAL
                     ATLKLFESADVLANNQRTAQTLLAAVNEYDFDRQHIRQRGMITAFEVSTDNPQFARDC
                     YAAALKHGLLLRPIGNTVYWMPPYCLTEADVSHLVQTTRAAVVDSQ"
     gene            4160..>5002
                     /locus_tag="VNZ84_08310"
     CDS             4160..>5002
                     /locus_tag="VNZ84_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HWU66999.1"
                     /translation="MQKTRKHLELLAPAKNADFGIEAINHGADAVYIGGPAFGARAKA
                     PNTVQDIARLVQHAHRFHAEVFVALNTIFHDNELEGVRQLVHQLYDSGVDALIVQDMG
                     LLELDLPPIQLHASTQTDIRTVEKAQFLDQVGFSQIVLARELDIATVKKIADATSANL
                     EYFIHGALCVAFSGQCFISHAHTGRSANRGECSQECRLPYTLEDQKGRIIGKDKHYLS
                     MKDNDQSANLRALIAAGVSSFKIEGRYKDLPYVKNATAHYRQLLDEILEEMPEYAKSS
                     AGHAS"
BASE COUNT         1251 a         1430 c         1137 g         1184 t
ORIGIN      
        1 atgattcacc atgtgcgtat gccaggcatc gacagcactc tcatccgtga gaacaaaacc
       61 gatatgatcc aggcgctgca gcggatcagc cttataatct acccggtgca aggcaaacac
      121 atccccatga ttggttaaat agacattatc aggatcaggt tgccactcga tatgcatacc
      181 aacaatgcgc gtataaaaat cgatcgccga ctcgaggtct ttgataaata acgccacgtg
      241 gcgtatgccc aacatcccgg caggaatcgt catcacttac tctacgctgt gcggtttgat
      301 ttcaaagtcg tgcgtaatct cgcatgcttt ttgcagcatg atactggccg agcaatattt
      361 ttctgccgac aggctcacgg cacgtgccac ttgcgactca ttaaggttat tgcctgtcac
      421 cacaaaatgc acatggatct tggtgaacac ttttgggatg gcatctgcgc gctcggcact
      481 gatttcagcc acacaatcca caatcggctg acgcgacttt ttcaaaatgg tcaccacgtc
      541 aaacgaagta caggcaccca tccctattag cagcatttcc atggggcgca tcccaatatt
      601 gcggccacca ttttcagggg cgccatcgag taccacggca tggccggtct cggactcgcc
      661 aacaaagctg acgccatcta tccatttcac ttggacttgc attttgactt tccaatcatt
      721 gaatttgact cattgcccac atcatctagt tggggacgcg atagcacaga cacaagtgac
      781 tcacatactc cctctgtcac ttgccccaaa cccgatgcac gcagaaaacg tttacttcac
      841 acccagcagc tcaacatcaa acaccaacgc tgcattgggt ggaatcacgc caccggcgcc
      901 acgtgcacca tagcccatgt cagatggaat aatcagggta cgcttgccgc caaccttcat
      961 gccttgcaca ccttcatccc agccctgaat gacctgacca ccgcccaaaa agaaattgaa
     1021 tggcgagttg cgatccaggc tgctatcaaa cttctgcccc ttgtggtcct ctttgttcgc
     1081 gtcatacaac caaccggtgt aatgtaccgt cacgttgaag ccgatctccg cttcacggcc
     1141 ttcacccacc accgtatcaa ttttttgtaa ttcagtcaca ttggcagaca tttgagcagc
     1201 ttccttagtt tttgaagaat tggattgcgc ttggcaacct gtcacagtca gcagcatcaa
     1261 catgctaaga ccgataattt tgattccaga tttcacttga tttactccca caaaacgacc
     1321 gttattttac gcgcttttga tcacaaattc tcggccaatt cagcgccctt cgatcaattg
     1381 ctttaatagt gtatcttgca ctgtacgcac tgccgaagcg cgtgcatgat tcgcaatcac
     1441 caccaacacg tagcgcttgc cagcactatc ttgcacatag cctgcaaggc tactcactcc
     1501 ttccagcgac ccggttttaa ggtacgccat gcctttgctt gcaccatctg ccaaccgttt
     1561 tttggtagtg ccatccatgc ccagtatcgg catagacgcc atgaagactg gcatcaccgg
     1621 acgttgccag gctgtgacga gcaactgcgc caaatgttcc gcactgatgc gttctatacg
     1681 tgacaaacca gacccgtttt caagcaccag ctctggaaat tgcaagccac gttgccgcaa
     1741 cacctgcttg agtaccagcg taccacccac ttcatctgct ggagtgaagc cagcctccag
     1801 ggcaatggtc aacagcagct ggcgcgccat cacattatta ctccacttgt tgatatcacg
     1861 cacaacatta tccagcggcg acgaccacca tgtggtgacc agattggtcg tatcaattgc
     1921 tggcgccagc aacagctgac cattaaactg cccacccaat tgcgcccaca gttttttaaa
     1981 actgaaaaac acatattgcg tgtctttaag cacactcaat tccagataac gctcatcaca
     2041 ctgttcgggc aaggtcccgc taaaaaccac tgtcgcaccg tccgcctgag gtgtgatgct
     2101 gtagtgcaca tccccgcgcc agtcattaca ggcgcccttg cgagtctgca tggtattgat
     2161 cacgcgaatt tgcggcaatt caaattcctg gctgatttgt accacaggct ttcctgccac
     2221 cttactggcc acaaagcgga agctggtatt gcggccattc agtaacaagg cactcgggtt
     2281 ggcgttataa gcgcgccagg gttcgtcgtc cagcaccggc cgctgactca cttcgggggc
     2341 ataggcggac aaatccagca ccaggttacc cctgatttga tggatgcctt gctgctgcac
     2401 caactgcaac aagcgccaga aatcactaat attgaatgca ggatctccgt agcccttaat
     2461 catcaaatca ccatccagta tgccttggct gacgggacca ttacggtaga tttcagtttt
     2521 ccaacgatag gctggcgtga gtgcttcgag ggctgcatag gtcgtgacca atttcatcac
     2581 gctggcagga ttcatggcct tggcagcatt gtggctaatc aggggtggtc cgccatcaac
     2641 cggctgcacc acgattgcca cgctttcggc gggtaattgc gctttgctta aggcctcttg
     2701 cagatcactg ggtaacgctg cctgcgccat gacggcccag caaacacata ttgcaagtac
     2761 ccagcgcatt actgactgtc caccacggca gcacgtgtgg tttgcaccag gtgcgacaca
     2821 tccgcctcag tcaaacaata gggcggcatc cagtacacgg tattgcctat tggccgtaac
     2881 agcaagccat gcttgagtgc cgctgcgtag caatcccgtg cgaactgcgg attatccgtg
     2941 ctgacttcaa acgccgtaat catgccacgt tggcgaatat gctgcctatc aaagtcgtat
     3001 tcattcacag ccgccagcaa agtctgcgct gtccgttgat tattcgcgag cacatccgca
     3061 gactcaaaca atttcaatgt ggccagcgcc gcactgcacg ccagtggatt accggtatag
     3121 ctatggctat gcaaaaaacc tttaacggtc tggtcgtgat aaaaggcctg ataaacctta
     3181 tccgtggtca acaccacgga caacggtaaa taccccccgg taatgccttt ggacaagcag
     3241 ataaagtcag caatatcgct cactttttcg cccgcctgct cacaggcaaa caaggtgccg
     3301 gtgcggccga acccgacggc gatttcatcg gcgatcaaat gcacttggta acggctgcaa
     3361 atttcgcgcg cccgttgcaa atacaccggg tgatacatcg ccatgcctgc tgcaccttgc
     3421 accaaaggtt cgataataaa tgcagccagg atggcatgat gctcggccac atagacttcc
     3481 agcgcctcag cacaacgtaa agcataggcc tcttccgttt cacccgcttc cgccagtcgg
     3541 ctatcggggc tttgcatttg cgcagactgc aaaatcagtg gcgcatacgt gtctttgaaa
     3601 atggcaacat cggtgacgct gagtgcaccc agggtctcgc cgtgataact attttgcagg
     3661 ctgataaatt tcgttttaag tggttgtccg atattgcgcc aataatgaaa actcatcttc
     3721 agtgcaatct cggtcgcact ggcaccatcc gagccataaa atgcgtgacc caagccagtt
     3781 aacgccgaga gtttttctga aagcgccacc actggttcgt gcgtaaaccc ggctagcatc
     3841 acatgctcca actgatcaag ctgggctttg attgcttctt taatcacggg ctggttatgc
     3901 ccaaataaat tgacccacca gctgctgacc gcatctagat aacccttacc gtcgcgatca
     3961 aacaaccagg caccttcgcc acgcgtaatt ggcaccaaag ggtaacgttc gtgatgtttc
     4021 atttgcgtgc atggatgcca cacgctgcgc aggctacgct gcaataaatc ctgatttcca
     4081 ctcatgacgc tattttaaac caagcgtaaa tagcatgctt ggtttaaaat agcgtcttaa
     4141 atcaagttct tacctctgca tgcaaaaaac ccgcaaacat ctcgaactgc tcgctccggc
     4201 aaaaaatgcc gattttggca ttgaagcgat taaccatggt gccgatgccg tgtatatcgg
     4261 cggcccggcc tttggcgcgc gtgctaaagc accaaatacc gtgcaggata ttgccaggtt
     4321 agtgcaacat gcgcatcgtt ttcatgctga agtatttgtc gcgttgaata ccatttttca
     4381 tgacaacgag ctcgaaggtg tacgccaatt ggtacatcag ctatacgaca gtggcgtcga
     4441 tgccctgatc gtgcaggaca tgggtttact agaactggac ttgccaccga tccagttgca
     4501 tgcgagtacg caaaccgata tccgtaccgt tgaaaaagca cagtttctgg atcaggtcgg
     4561 ctttagccag atcgtactgg cacgcgaact ggatattgcc acggtgaaga aaatcgccga
     4621 tgccacctcg gccaacctgg aatactttat tcacggcgca ctttgcgtcg cgtttagcgg
     4681 ccagtgtttt atcagccacg cgcataccgg ccgcagtgcc aaccgtgggg aatgctcgca
     4741 agaatgccgc ttgccttaca cactggaaga ccagaaaggc cgcatcatcg gcaaagacaa
     4801 acattacctg tcgatgaaag acaatgatca gagcgccaat ttgcgcgccc tgatcgcagc
     4861 tggtgtcagc agcttcaaga ttgaaggccg ctataaagat ttgccgtatg taaaaaacgc
     4921 cactgcgcat taccgccaac tacttgatga gattctagaa gaaatgccgg aatacgcgaa
     4981 gtcttccgcc gggcatgcta gc
//
LOCUS       DATMSK010000251         6919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_208803,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000251 DATMSK010000000
VERSION     DATMSK010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208803"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..2345
                     /locus_tag="VNZ84_08315"
     CDS             21..2345
                     /locus_tag="VNZ84_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HWU67000.1"
                     /translation="MSQPKASIKPYHHPAAGWGALKQVAAQLALQKIPVKGVMTLLAQ
                     NQPDGFDCPGCAWPDKEHTSTFEFCENGVKAVAAEATAKRTPPEFFAKHTVTELMNWS
                     DFDLENEGRLTHPMRYNPVTDKYEVIAWDDAFALIAEHLNALADPNEAIFYTSGRAGN
                     EAAFLYQLFVREFGTNNFPDCSNLCHESTSIALPATVGIGKGSVTLHDFEHADTILIF
                     GQNPATNHPRMLGELREAAKRGARIVAINPLKERGLERFADPQSPFDMLSFKGSKISD
                     LFIQPKLGGDLALIKGMIKFVIQWDDEAILHGNERVVDLEFIEQHTNNFEAIAEDARQ
                     ESWDVIVQESGVPLDQIEEIARIYATGDRVISTWGMGITQHKHGVATIKTLVNLMLLR
                     GNIGKEGAGLCPVRGHSNVQGNRTVGIYEKMPAEFLESLERVFDIKVPRTPGYDAVAA
                     IQAMIDGKGKVFFGLGGNFSIATPDTDRTWEGMRNCDLTAHVTTKLNRSHLVHGKEAL
                     ILPCLGRTEIDTQESGPQGVTVEDSMSMVHISYGMNKPASPHLLSEPAIVARLAHATL
                     KHSKTPWLEFIKDYSKIRDAIEQTMPVAFKDYNERIKVPGGFRMPVPSSERVWNTLSG
                     KAEFTLQAIPQNSPIHQAREQHGDQLFVLMTTRSHDQYNTTIYGMDDRYRGVFGQRRV
                     IFMNPADIARMNLAAGDWINLVSVWQDGVTRRADHFMVVAYDIPQGCLSGYYPETNNL
                     VPLESVADQSRTPTSKSIPVMIEKATAPEVANAA"
     gene            2335..2649
                     /locus_tag="VNZ84_08320"
     CDS             2335..2649
                     /locus_tag="VNZ84_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67001.1"
                     /translation="MLLESDHALESFQDDVISECMPLDRSTVLMLSILELLHAEHAQG
                     RPYVSFAVICKRLEVRMSTLQRLMTALNEQALVDVITQKDRLVAALTTGGEEVSLALQ
                     AA"
     gene            2739..4190
                     /locus_tag="VNZ84_08325"
     CDS             2739..4190
                     /locus_tag="VNZ84_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWU67002.1"
                     /translation="MKLYVSFAARIRAMIDEGVLRPGDRIFSVRQASQKYELSISTIL
                     RAYLLLEQEGVISSHPQSGYYVTPRKRLQQRHGSPLAKDINLSEIDASKLVLTTLKSI
                     REFGTVPLGSPFPDPQLFPLKKIHHYEKALTDDQGEWGVLSDLPPGNEMLRQQIARRY
                     LANGMDVSPDDIVITHGATEAITLCLQAVAKAGDTIALESPGYYALAHTVERLGMKVA
                     EIRTDPASGIDLDALRAVTDNVHIAAVILTSNFQNPLGFVMPEDKKRALVDFLTEQNI
                     PLIEDDVYNELYFSDEPPLPAKVFDRAGIVLHCSSFSKSLAPGYRVGWTTAGRYRQEV
                     ERLMFLNSLSLTSAPQIAIAKFMQRDSFEQHLKQLRHTLRSNYVLMRNVIEQSFPAGT
                     QLSIPQGGYVIWVKLPAVLDALELYRAAIDNGITVAPGTIFSRKKELTHYIRLNFSHL
                     WTLAIEQAVRELGALACRMIATAKTGEDSATNT"
     gene            complement(4229..4714)
                     /locus_tag="VNZ84_08330"
     CDS             complement(4229..4714)
                     /locus_tag="VNZ84_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU67003.1"
                     /translation="MTIPAKNTVCLWYDGCAEEAASFYAATFPDSSVDAVYRAPADFP
                     SGKEGDVLTVAFTVMGIPCLGLNGGPGVNHNWAFSFQVATSNQDETDRYWAAITSNGG
                     EESECGWCKDKWGVFWQITPTALTQAITDSDATAAKRAFEAMLSMKKIEIAVIEAALR
                     G"
     gene            5047..>6919
                     /locus_tag="VNZ84_08335"
     CDS             5047..>6919
                     /locus_tag="VNZ84_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWU67004.1"
                     /translation="MQSKKQEFWALESIIIYGFISFFVLLVVLGITSIIYLQRFAENN
                     RWVSHTHAVISNLDRGLVLFNQLEVSQRGFLLTGDSGSISRRDAALVDLQKEIAKLTV
                     LTKDNKFQQEQISQLRRLIDRRVIYFNQLNEVARATPVDQLKELLRKDFIRREDMLEI
                     RGVVSAMSLEEQSLLSRRTQSEQEHAKDLLILFAVMSVAILIFFPLLIWRIQRDLNIR
                     KRAATEQKRLTDILDASPDLIANKSLQGDYFYLNSGGRKLLGVGENEDIQSQPISRMR
                     PAWAAELIRNQAIPTALSTGVWRGETAYISRAGIEIPVSQVIVAHRDAEGVATHLSTV
                     ARNISDFKQIEYDLMNAAVYDQTHSEVLRLFNANYDRAHILPSMLEILARNHAFPVSA
                     VYGYDEWSGFYRLESAHAAPIDILHEFKLGHGVIGEAIQRNHTLILGDLDELPSLTIE
                     TGLLSLKPAGIIICPVSYQEKRLAVLVLAASKPITERDQTFVERLSAQLGVALHNIQQ
                     YGDLKLLAEQLRLRSEEIGLKNEQLLEADRMKSEFLATMSHELRTPLNAIIGFSEILR
                     DGLIGPLADKQKAYINDIFESGQHLLSLINDVLCRIGGCALFGDRHMATQSRSALAAY
                     "
BASE COUNT         1647 a         1753 c         1848 g         1671 t
ORIGIN      
        1 cactcaccct agtaggacat atgtcgcaac ctaaagcttc cattaagcca tatcaccatc
       61 ccgccgccgg ttggggggca ttaaaacaag tcgccgcaca actggcattg cagaagatcc
      121 cggtcaaagg cgtaatgacc ttgctggcgc agaaccaacc agatggtttt gattgcccgg
      181 gttgtgcctg gccggataaa gaacatacct ctacgttcga gttttgtgag aatggtgtga
      241 aggccgtggc ggccgaggcg actgccaagc gcacgccccc tgagtttttt gccaaacaca
      301 ctgtgactga gttgatgaac tggagtgatt tcgacctcga aaacgaaggc cgcctgacgc
      361 atcctatgcg ttacaacccg gtgactgaca aatatgaggt gatcgcttgg gacgacgcat
      421 ttgcattaat cgctgaacat ttgaatgcct tggccgaccc caatgaggcg attttctata
      481 cctcaggccg tgccggtaat gaggcagcat tcctgtacca gttatttgtt cgtgagttcg
      541 gtaccaataa cttccctgat tgctccaact tgtgccacga gtccaccagc atcgccttgc
      601 cggccaccgt aggtataggc aagggttcag tcactttgca tgactttgag cacgccgaca
      661 ctattctgat ttttggccag aacccggcca ccaatcatcc acgcatgttg ggtgaattgc
      721 gtgaagcggc caagcgtggc gcgcggattg tggcgattaa tccattaaaa gaacgcggcc
      781 tggaacgctt cgctgatccg caaagcccgt ttgatatgtt gtctttcaaa ggcagcaaga
      841 tcagtgacct gtttattcag cccaagctgg gcggtgattt ggcgctaatc aaaggcatga
      901 tcaagtttgt gatccagtgg gatgacgaag caatcctgca tggtaatgag cgggtggtcg
      961 acctggagtt tattgagcag cacaccaaca actttgaggc gattgccgaa gatgcacgcc
     1021 aagaaagctg ggatgtgatt gtgcaagagt ccggggtgcc attagaccag attgaggaaa
     1081 ttgcgcgcat ttatgccacg ggcgatcgcg tgatttcgac ctggggcatg ggcatcaccc
     1141 agcacaagca tggcgttgct accatcaaga ccctggtcaa cctgatgttg ttgcgcggta
     1201 atatcggcaa ggaaggcgca ggcctgtgcc cggtacgcgg ccatagcaat gtgcagggta
     1261 accgcactgt gggtatttac gaaaaaatgc cagcagagtt cctcgaaagc ctggagcgcg
     1321 tgtttgatat caaggtcccc aggacgcctg gatacgatgc cgtcgccgcc attcaggcca
     1381 tgatcgatgg taaaggcaag gtgttttttg gtttgggagg taatttctcc atagccacgc
     1441 ctgatactga ccgtacttgg gaaggcatgc gcaattgtga tctgactgcg catgtcacca
     1501 ccaagctgaa tcgcagccac ctggtacatg gtaaagaagc attgatcttg ccatgcctgg
     1561 gccggactga aattgacacg caggaaagtg gcccgcaggg cgtcaccgtg gaagactcca
     1621 tgagcatggt gcatatttct tacggcatga acaaaccggc gtcgccacac ttgttgtccg
     1681 aacctgccat cgtggcacgc ctggcacatg ccacactcaa acacagcaaa acgccctggt
     1741 tggagttcat taaagactac agcaaaatcc gcgatgcgat tgagcaaacc atgccagtgg
     1801 cttttaagga ttacaacgag cgtatcaagg tgccgggtgg cttccgtatg ccggtgcctt
     1861 ccagtgagcg agtctggaat accctttccg gtaaggctga atttaccttg caagcgattc
     1921 cgcagaattc acctattcat caggcgcgtg agcaacacgg tgaccagcta tttgtactga
     1981 tgaccacgcg ctcacacgac cagtacaaca ccacgattta tggcatggat gaccgttacc
     2041 gtggcgtatt cggccagcgc cgtgtgattt tcatgaaccc ggcagacatt gcgcgtatga
     2101 acctggcggc gggcgactgg atcaacctgg tcagcgtctg gcaggatggc gtgacgcgcc
     2161 gggctgatca ttttatggtg gtggcttacg atattccgca aggttgcctg agtggctatt
     2221 acccggaaac caacaatttg gtaccgctgg aaagtgtggc cgatcaatcg cgtacgccga
     2281 cttcaaagtc aattcctgtc atgattgaaa aagcgactgc accagaggtg gccaatgctg
     2341 cttgagtcgg atcatgcact ggagtcgttc caggatgatg tcatctcgga gtgcatgccg
     2401 ctggacagga gcaccgtgct catgctgagc atcctggagt tgctgcacgc tgaacacgca
     2461 caaggacgcc cctacgtttc tttcgccgtg atctgcaaac ggctggaggt gcgcatgagt
     2521 acactgcaac gcctgatgac ggcattaaac gaacaggcgc tggtcgatgt gatcacgcaa
     2581 aaagatcggc tggtggcggc gttgacgacg ggtggcgagg aagtctccct ggcattgcag
     2641 gctgcttaac cagccctcgt cttcgcgtta aacacccgcc gtacctcgca tgcctggagt
     2701 ataatcaggt atcaaaactc cttcctgcct ggccattcat gaagctttat gtctcgttcg
     2761 ctgcccgtat ccgtgcgatg attgatgaag gcgttctcag gccgggtgac cgaatctttt
     2821 cggtacggca ggccagccag aaatatgaac tcagtatcag cacaattttg cgtgcctatc
     2881 tgctgttaga gcaggagggt gtgatcagca gccaccctca atccggttac tatgtcaccc
     2941 cacgcaaacg cctgcaacaa cggcatggct ctccgctggc caaggatatc aacctttccg
     3001 aaattgacgc cagcaaactg gtgctcacta cgctgaaatc tatccgtgag tttggcactg
     3061 tgcctctagg ttcgcccttc ccggatccgc agttatttcc gcttaaaaag attcaccatt
     3121 atgaaaaagc actgaccgat gatcagggcg aatggggcgt actcagtgat ttgccgcccg
     3181 gcaatgaaat gttgcgccaa cagattgcac gccgctacct ggccaatggc atggatgtgt
     3241 cgccggatga tattgtcatc acgcatggcg caaccgaggc gattacactt tgcctgcagg
     3301 cggtggccaa ggcgggagat acgattgcac tggagtcacc aggctattat gcgctggccc
     3361 ataccgtaga gcgtctgggc atgaaagtcg ctgaaatacg aacagatcct gcgtctggga
     3421 tagatctgga tgccttgcgt gccgtgactg acaatgtgca tatcgccgcg gtgatcctca
     3481 ccagtaattt ccagaatccg ctgggctttg tcatgccgga agacaaaaag cgggcattgg
     3541 tagattttct aactgagcag aatatcccgc tgattgaaga tgatgtctac aacgagcttt
     3601 attttagtga cgaaccgcct ttgcctgcca aagtgtttga ccgtgcaggc atcgtgttgc
     3661 attgttcgtc gttttcgaaa tcgctggccc ctggctaccg cgtgggttgg accacggctg
     3721 gccgctacag gcaggaagtt gaacgtctga tgtttctcaa tagcctgtca ctgacttctg
     3781 caccgcaaat cgccattgcc aagtttatgc agcgcgacag tttcgagcag catcttaaac
     3841 agttaaggca taccttacgc tccaactatg tcctcatgcg taatgtgatt gaacaaagct
     3901 tcccggccgg gacccagctt tctattccac aaggcggtta tgtcatctgg gtcaaactgc
     3961 cggcggtgct ggatgcactt gagctatacc gggccgccat cgacaatggc atcacagtcg
     4021 caccgggcac gattttctcg cgaaaaaagg agctgacgca ctacatccgc cttaacttca
     4081 gccacttatg gaccctggcg atcgagcaag ccgtgcgtga gcttggtgcg ctagcatgcc
     4141 ggatgattgc cacagcaaaa acgggtgagg atagcgctac caacacatag ccgccgcctt
     4201 tcagcctctg agcggttgac tgggatggtt agccgcgtag ggcggcttca atgacagcga
     4261 tttctatttt tttcatggag agcattgctt cgaaggctct tttggccgcc gtagcatcac
     4321 tatccgtgat cgcttgggtt agcgccgttg gtgtaatctg ccagaataca ccccatttat
     4381 ccttgcacca gccgcattcg ctctcttcac caccattgct ggtaatcgcc gcccaataac
     4441 gatctgtttc atcctgattg gacgtagcga cctgaaatga gaatgcccag ttgtggttga
     4501 cgcccggacc gccgttgagc cccaggcagg ggatgcccat gacggtgaat gcgaccgtta
     4561 atacatctcc ttcttttcct gagggaaagt cagcgggggc acgatatacc gcgtcaacgg
     4621 aagagtctgg aaaggtggcc gcataaaagc ttgccgcttc ttcagcgcag ccgtcatacc
     4681 aaaggcatac tgtatttttg gcagggattg tcatattcat tctccgtgat gtactaaaaa
     4741 gtacgatcaa agcgcatgtg gttaagtggc aggtcactca tggcgaatca cttgtcgcct
     4801 gatcaatctg gccatagcct ctcgcgtttc tcggccggac acagggcgcg cccgtaaacg
     4861 gcacgtcaat ttcatatacg atttgcatcg caagtcagcc ttgtaaacat attactgtaa
     4921 atactcttac gtcttttatc aatttagtat ttaataacac ctgatggtgc aatagctgaa
     4981 ctgattcatc ctgaagttaa aaaaacacgg gggacgtcgc aatgctaatc gctgggtttc
     5041 atcgctgtgc agtctaaaaa gcaagagttt tgggcgctgg agtccatcat catttatgga
     5101 ttcatcagct tttttgtgtt gcttgttgtg ctgggcatta ccagcatcat ttatctccag
     5161 cggtttgcag aaaataaccg atgggtgagt catacgcatg ccgtcatttc caaccttgat
     5221 cgcggattgg tgttattcaa tcagctggaa gtctcgcaac gggggttctt gctgactggc
     5281 gatagtggat ccatttcccg tcgcgatgct gccctggtcg acttgcaaaa agagattgca
     5341 aaactgaccg tactcaccaa agacaataag tttcagcagg agcagatttc ccagttaagg
     5401 cggttgattg accggcgtgt gatttacttt aaccagctga atgaagttgc acgcgcaacg
     5461 cctgtcgatc agttaaagga actgctacgc aaggacttta tccgcaggga ggatatgctg
     5521 gaaattcggg gcgtggtgag tgccatgtcg ctggaagagc agagcttgct ttcccgccgc
     5581 acccaatcag aacaagagca tgccaaagat ttgctgatac ttttcgcggt gatgtctgtt
     5641 gctatcctga ttttttttcc attgctgatc tggcgtatcc agcgtgacct caatatccgc
     5701 aaacgggctg cgaccgaaca aaaacgcctg acagatattc ttgacgcctc gcctgacctt
     5761 attgcgaaca agagcctgca aggcgactat ttttatttga atagcggcgg gcgcaagctg
     5821 ctgggagtcg gtgaaaatga agatatccaa tcccagccaa tttcacgtat gcgtcccgct
     5881 tgggcggctg agttaattcg caaccaagcg attccaaccg cactgagcac gggtgtctgg
     5941 cgaggcgaaa cggcttacat ctcccgtgca ggcattgaaa tcccagtgtc ccaggttatt
     6001 gttgcgcatc gggatgcaga gggcgttgct acgcatttat ctaccgtagc ccgcaatatc
     6061 agcgatttca agcagattga atatgatctg atgaatgcgg cggtctatga tcaaacacac
     6121 agtgaagtat taaggctgtt taatgccaat tatgatcgtg cacatatctt gccgagcatg
     6181 ctggaaattt tagccagaaa tcatgccttc ccggtttctg cagtgtatgg ttatgacgaa
     6241 tggagcggat tttaccgcct ggagagtgcg catgcggcgc cgatagatat tctccatgag
     6301 ttcaagctgg gacatggcgt gataggggag gcgattcaaa gaaatcatac cttgatactg
     6361 ggcgatctgg atgagctccc gtcactgacc attgaaaccg gcttgttatc gctgaaacct
     6421 gcgggaatta ttatttgtcc ggtaagttac caggaaaagc gactggcggt actggtgctg
     6481 gcagcaagca agcccattac ggagcgcgat caaacgtttg ttgaacgttt gtcagcccaa
     6541 ttgggggtgg cattgcataa tatccagcag tacggcgacc tcaagctttt ggcggaacag
     6601 ctacggctac gtagtgagga aatagggtta aagaacgagc aattgctgga agcggacaga
     6661 atgaagtccg aattccttgc caccatgtcg cacgaattac gcacgccgct caatgccatc
     6721 attggttttt cagagatatt gcgtgatggt ttaatcggac cgctggccga taagcaaaaa
     6781 gcctatatca atgatatttt tgaaagcggg caacatttac tttcgctgat taatgacgtg
     6841 ctatgtcgta ttggtggatg cgccctgttc ggggaccggc acatggcgac gcaatcccga
     6901 agcgcgctgg cggcttaca
//
LOCUS       DATMSK010000252         5246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_19638,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000252 DATMSK010000000
VERSION     DATMSK010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19638"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1073
                     /gene="cysM"
                     /locus_tag="VNZ84_08340"
     CDS             180..1073
                     /gene="cysM"
                     /locus_tag="VNZ84_08340"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HWU67005.1"
                     /translation="MKPFKTLDDFVGNTPLVALQRMNPNPSNTILLKLEGNNPAGSVK
                     DRPAYSMILRAEQRGQIKPGDTLIEATSGNTGIALAMVAAMRGYKMVLVMPENQSVER
                     RQSMKAYGAELVLTPKDGSMELARDVALKLQAEGEGIVLDQFGNSDNPLAHYEGTGPE
                     IWRDTEGKVTHFVSSMGTTGTIMGTSRFLKEQNPNIQIIGVQPEEGSQIAGIRKWPEE
                     YLPTIYDPARVDQFRYVSQKHAEDTTRKLAVKEGIFAGVSSGGALYTALQLSKEVENA
                     VIVSIVCDRGDRYLSTGVFPG"
     gene            1073..3058
                     /locus_tag="VNZ84_08345"
     CDS             1073..3058
                     /locus_tag="VNZ84_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67006.1"
                     /translation="MRIWGLMLWAWALPLSAMTEISLSAESISSDLFQLNKPHAVVNL
                     NSKHVQVQADRLQLGSASLDKPNIRLDMGARPTLLVASEQLQMQPYQVRQPRIFLDLR
                     PQSQQPVLSFYAEVKALQDEVWGTFHLNCQVPQQPASQTWRCEDGVYHDVRSHIPFNV
                     LVTPTWKDQDKQGPGARGVDIEMSVREAKFSDAAGLHAGDKLTGKINLSAHEQAGGWR
                     WQGVFQWQQGELFWQPFYFAEGNKRFEIRGFYRQPYVDIEQSTLELQGVGTLHSQSRL
                     NLVNKQFEFLRVNATDVDFNGVYQAFIQPLIPHSAFGQLQVSGKADWSFEAKGLQPLK
                     FHLKIADASVEEQTGKFGFAHFNADIPWDYDHPQPITMGYQSGHILKIPLGETKWQAE
                     VNRYSITSPSLRLPILDGALDFQDISAAWINQSMVWHVKMAMQPISMSSFSQALEWPA
                     MRGQITGTIPLVTYANRELRMDGEMQFKLFNGVVGMSDLDIDDPLGAVPKLHASFSMR
                     DIDLGEITRTFNFGSISGKLEGDIKDLRLQNWKPVSMNAFVRTADGPFEKKISQRAVE
                     NITALGGVGTAAALQRTFLRFFKEFGYEKIGLSCELRGDICKMGGVDQVPGGFVIVKG
                     KGAPSVNVNGYTQYVSWKDVLARMRRVTDSNSKIIID"
     gene            3143..3379
                     /locus_tag="VNZ84_08350"
     CDS             3143..3379
                     /locus_tag="VNZ84_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67007.1"
                     /translation="MRNSGLVLATTGLCACVTINIYFPAAAAEKAADKVIDEIWQTPA
                     GQSPAASPSSVPTAAPVTPSATPVTPSVNQEVTP"
     gene            3376..3843
                     /locus_tag="VNZ84_08355"
     CDS             3376..3843
                     /locus_tag="VNZ84_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HWU67008.1"
                     /translation="MRYVQSGLVQLFLHHGYQSLKQVLLGVCLTTLLIGTAHAAADLD
                     VNTPAVAAVKASMQNRHLQLLPHYQSGAVGLTAEGFVAVKDAGLVPLSQRSALAALVK
                     DENMDRAQLYHGIAAANGHPEWEGEIQRTFALRWIDKAQTGWFVQRDGQWVRK"
     gene            4025..4504
                     /locus_tag="VNZ84_08360"
     CDS             4025..4504
                     /locus_tag="VNZ84_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67009.1"
                     /translation="MATNLFVLPRLGESIHQQDLGIYYILNHHTGRVRSLIQLVRENV
                     HWYLMQLQPIADGLDYRWSVLSREEDCTLKSATDKQIAHYFSKPEYAEPYGAWQVMRN
                     ANFGFAKFTPMEEPHEVSYAILTFDGEDMQTPIRLHKAPPEWLDEDSETDILQLEFA"
     gene            complement(4565..>5246)
                     /locus_tag="VNZ84_08365"
     CDS             complement(4565..>5246)
                     /locus_tag="VNZ84_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67010.1"
                     /translation="LIMTSISSLGSSNSIQSFSQIGQRSGMQPPPPPPDGEDQGKDGG
                     GLLGAIDSALKSAGIEGGLESIFGQSGTSSTSASSDSSETTDSSDSTTDAASALNNFL
                     QNLISALQSQGTTDTGSSDSEESSTSSTEASAAQGMPPPPPYGGHGMEAKLQSLISSL
                     SSDSTDSTATDTSSDSSSSELESSFQSLVSALGGNSDSASLSGFLQALSSGMQHRGPA
                     GNVVSTTA"
BASE COUNT         1280 a         1227 c         1423 g         1316 t
ORIGIN      
        1 gcaattatta tttttgtgat aggggtagtt tatgtaaaat tggtacttcg caagctaaat
       61 cagtaatgac attggaattt tacatatatt aagctgcggc gtgtattact tttaatcaat
      121 ccagaatatt ttgggaacca ggcccctttc cgcctggaaa taacgaacat taatcagtca
      181 tgaaaccatt taaaactttg gatgactttg tcggtaatac gccgctggtc gctttgcaac
      241 gcatgaatcc caatcccagc aataccatat tgctcaagct ggaaggcaat aatccggccg
      301 gctcagtcaa agaccggcca gcgtacagca tgattctacg tgctgaacaa cgcggccaga
      361 tcaagccagg cgacacgctg attgaagcga ccagtggtaa taccggcatt gcactggcca
      421 tggtggcggc catgcgtggc tataaaatgg tattggtcat gccggagaac cagtcagttg
      481 agcgcaggca aagtatgaaa gcgtatgggg ctgagctggt tttgacaccc aaggacggta
      541 gtatggagct ggcgcgtgat gtcgctctca agttgcaggc cgaaggcgaa ggcattgtgc
      601 tggatcagtt cggtaattcg gataacccgc tggcccacta tgaagggacc gggccggaga
      661 tctggcgcga tactgaagga aaagtgacgc attttgtttc cagcatggga acgaccggga
      721 cgattatggg gacgtcacgt tttctcaaag aacaaaatcc gaatattcag attattggtg
      781 tacaacccga agagggatcg cagattgctg gcatccgcaa atggccggaa gaatacctgc
      841 ccacgattta tgacccggca cgcgtagacc agtttcgtta tgtcagccag aaacatgccg
      901 aggacaccac acgcaagctt gccgtcaaag aaggtatttt tgccggagtg tccagcggag
      961 gcgccctgta cacggcgctg caattatcta aagaggtcga gaatgccgtg attgtgagca
     1021 tcgtttgcga ccggggtgac cgttacctct ctaccggagt gtttccaggg taatgcgcat
     1081 ctgggggctg atgctatggg cttgggcgtt gccgttaagt gcgatgaccg agatcagcct
     1141 cagcgccgag agtatttcca gtgacctgtt ccagctcaat aagccgcacg ccgtcgtcaa
     1201 tttaaacagc aagcatgtgc aggtgcaggc ggaccgtttg caacttggta gtgccagtct
     1261 ggataaacct aatatccggc tggatatggg cgctcggccc acgttgctgg tggcctctga
     1321 gcagttgcaa atgcagcctt accaggttcg acagcccagg atatttcttg atttgcggcc
     1381 gcaaagccaa cagcctgtat tgtcttttta tgcggaggtg aaagcattgc aggatgaagt
     1441 ttgggggacg tttcacctga actgccaggt gccacaacag cccgcaagcc aaacctggcg
     1501 ttgtgaagat ggcgtatacc atgatgtacg ttcccatatc ccatttaatg tgcttgtgac
     1561 acctacctgg aaagaccagg ataaacaggg acctggggcc aggggtgttg atatcgagat
     1621 gtcagtgcgg gaggcaaaat ttagcgatgc tgctgggttg catgccgggg acaagctcac
     1681 aggcaagatt aacctttctg cgcatgaaca ggccggtggc tggcgttggc agggcgtgtt
     1741 ccagtggcag cagggggagt tgttctggca gccgttttac tttgccgagg gcaacaaacg
     1801 gtttgaaatt cgcgggtttt accgccaacc ctatgtggat atcgaacaat ctacgctgga
     1861 attgcaaggt gtaggcacgt tacatagcca gagccgcctt aacctcgtca acaagcagtt
     1921 tgagttttta agggtgaatg cgaccgatgt ggattttaat ggcgtttacc aggcgtttat
     1981 ccagcctttg atcccgcatt ctgcctttgg gcagttgcag gtatcaggca aggcagattg
     2041 gtcgtttgag gccaaaggtc tgcagccact gaaatttcat ctgaaaattg cggatgccag
     2101 cgtggaagag caaacgggca agtttggctt tgcccatttt aatgcggata ttccctggga
     2161 ttacgaccat ccgcaaccta ttaccatggg ataccagtcc ggacatattt taaaaattcc
     2221 actcggtgaa accaaatggc aagcggaagt aaaccgctac tccattacgt cgcccagttt
     2281 aaggctgccg atattggatg gcgctttgga ttttcaggat atctccgctg cgtggattaa
     2341 ccagagcatg gtctggcacg tgaaaatggc catgcaacct atcagcatgt ccagttttag
     2401 ccaggcgttg gagtggccag ccatgcgcgg gcagatcacc ggaacaattc ctttggtcac
     2461 ttatgccaac cgtgagttac gcatggatgg cgagatgcaa ttcaagttgt ttaatggcgt
     2521 ggtggggatg tctgacctgg atatcgatga tcctttgggt gcagtgccca agttgcatgc
     2581 cagtttcagt atgcgtgata ttgatcttgg cgaaattacg cgtacgttta attttggttc
     2641 tatcagcggt aagctcgaag gcgatatcaa ggatttgcgg ttgcaaaact ggaagcccgt
     2701 ttctatgaat gcttttgtac gtactgcaga tggtccattt gagaaaaaaa tctcgcagcg
     2761 cgccgtcgaa aacattacgg cgctaggtgg cgtgggcact gcagctgctt tgcaacgcac
     2821 ctttctacgc ttttttaaag aatttggata tgaaaaaatt gggctgagtt gcgagttacg
     2881 tggtgatatt tgcaaaatgg gcggtgtaga ccaggtacct ggcgggttcg tgattgtcaa
     2941 aggtaagggt gcgccgtcag tcaacgtcaa tgggtatacc caatatgtca gttggaaaga
     3001 tgtcttggcg cgcatgcggc gcgtgacaga tagcaatagt aaaatcatta ttgactagac
     3061 ttgagtggaa aaagcggaca tcgtttgccg gaaaggaatc aaattggacc cgactgtgat
     3121 caataagcat gcgttgcgct ggctgcgcaa tagtggcttg gtactggcaa ccacaggcct
     3181 gtgtgcctgt gtgacgatta atatttactt tccggctgct gcggcagaaa aggcggcaga
     3241 taaagtcata gacgaaatct ggcaaacgcc tgcgggacaa tctccagccg ctagtccctc
     3301 ttcagtgccg acagcggcgc ctgtcacgcc ttcagcaacg cctgttacgc catcagtgaa
     3361 ccaggaggtt acgccatgag atacgtccaa tcaggcttgg tgcaactatt tttgcatcat
     3421 ggctatcaga gtttaaaaca agtattactg ggagtgtgcc tcacgacact gttgataggc
     3481 accgcgcacg ctgcggctga cctggacgtg aatacaccag ctgttgccgc agtaaaagcc
     3541 agcatgcaaa accggcattt gcaattattg ccgcattatc agtctggtgc cgtgggctta
     3601 actgcagaag ggtttgtggc agtaaaagat gccggattgg tgcctttgtc ccaacgtagt
     3661 gcgttagcgg cactggttaa agacgaaaat atggacaggg cgcagttata tcacggcatt
     3721 gctgccgcaa atggtcaccc tgagtgggaa ggcgaaatac aacgtacgtt tgccctgcgc
     3781 tggatagata aagcgcaaac tggctggttt gtgcagcgcg acgggcaatg ggtgcgtaaa
     3841 taagctggca aaacaacggc ttggcattgt cgacttaaat gaacgcctga atatttcccg
     3901 cgagggtgcg ctggccccag tgcagcgcaa ttcaataaac agcaccagaa aagcctgtta
     3961 tttaagtttc aggcatattc attgcataca gtttaagtgc gtgttccaat gaaaggagtc
     4021 tgttgtggca actaatcttt tcgtactccc taggctgggt gaaagcattc atcaacagga
     4081 tttgggaatt tactatatcc tcaatcacca tactggccgt gtgcgttcct tgatacagct
     4141 ggtgcgtgaa aacgtacatt ggtatctcat gcaactgcaa ccgattgccg acggcctgga
     4201 ttatcgctgg tctgtcttgt cgcgtgaaga ggattgcacc cttaaatcag caaccgacaa
     4261 acagattgcc cattacttct caaagccgga atatgcagaa ccctacggtg cctggcaggt
     4321 gatgcgtaac gccaattttg gttttgccaa gtttacgccg atggaggagc cacatgaggt
     4381 cagttatgcg atcctcacct ttgatggtga agacatgcaa acgcctatcc gcctacataa
     4441 agcacctccg gaatggctgg atgaagattc tgagaccgat attttgcaat tggaatttgc
     4501 ttgatctctg tttgaagtag caactaccac acctggtggt agctgccacc ctatgcaacc
     4561 ttgattacgc cgtggtgctg actacattac ctgctggccc gcgatgttgc atgccggaag
     4621 ataatgcttg caagaaaccg ctgaggctag cgctatcgct gttgccaccc aaagcactca
     4681 ccagtgactg gaaactgctt tccaattcac tgctactgct gtctgaagac gtatcggttg
     4741 ccgtgctgtc ggtactatcg ctactcaagg aagaaataag actttgtaat ttggcttcca
     4801 taccatgtcc gccataaggt ggcggaggcg gcataccctg tgctgcgctt gcttctgtac
     4861 tacttgtgct ggactcttca ctgtcactac ttccagtgtc cgttgttcct tgcgattgca
     4921 aggcactgat caggttttgt aaaaagttat tcagcgcact ggccgcatct gtggtgctgt
     4981 cgctgctgtc agtcgtttcg ctactgtctg agctggcact ggtgctgctg gtacctgact
     5041 gaccaaagat tgactccagc ccgccctcga tgcctgcgct tttcaacgca gagtcaatcg
     5101 cccctaataa gccgccaccg tctttgcctt gatcttcgcc atctggcggg ggagggggag
     5161 gttgcatgcc actgcgctgg ccgatctgcg aaaatgattg aatactgtta ctactaccta
     5221 gtgaactgat agatgtcatg atgagt
//
LOCUS       DATMSK010000253         7834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_160366,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000253 DATMSK010000000
VERSION     DATMSK010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160366"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VNZ84_08370"
     CDS             <1..288
                     /locus_tag="VNZ84_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67011.1"
                     /translation="IELMIVVAIIGILASVAIPAYQDYTKDAADNACAVQTKGFTNNY
                     VLAQQTNRTLPTSTAGACRDVATITATQISAQAKAPGDKTTTCIIATGVCS"
     gene            390..713
                     /locus_tag="VNZ84_08375"
     CDS             390..713
                     /locus_tag="VNZ84_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWU67012.1"
                     /translation="MRNFQKGFTLIELMIVVAIIGILASIGIPLYQDYTKDAANNACA
                     VQTKQFVDNYNLASQTNKSLPTSIAGACADAAIITSTSITATAKYPGSKTTTCTIATG
                     ACITS"
     gene            879..1529
                     /locus_tag="VNZ84_08380"
     CDS             879..1529
                     /locus_tag="VNZ84_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWU67013.1"
                     /translation="MKEAIIIDSKKHLATVFWLHGLGADGHDFEPVVQMLNLPHIKFV
                     LPHAPYRPVTLNNGYEMRAWYDIFGLQSESQQDEVGITQMQATINSMIAAEIALSIPS
                     QRILLAGFSQGGAIALHTATRFDQPLAGVLALSTYLPLKHQLKEKQHPANQQLPIWMA
                     HGRQDSVITLSTAASSRQTLEAAEYKVEWHEYDMPHSVCEAEINDIREFLLRVLPA"
     gene            1610..1852
                     /gene="xseB"
                     /locus_tag="VNZ84_08385"
     CDS             1610..1852
                     /gene="xseB"
                     /locus_tag="VNZ84_08385"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWU67014.1"
                     /translation="MSASKKSPSAVSDQQTFAERLAALDNIVRSMESGELPLEAALTA
                     YTEGMQLLQACQESLQQAEQTVMILNQQQLTPFEPN"
     gene            1877..2767
                     /locus_tag="VNZ84_08390"
     CDS             1877..2767
                     /locus_tag="VNZ84_08390"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HWU67015.1"
                     /translation="MSTPAFVFDHWAAAHQHRVEEVLVRTLPVNAILPERLHEAMRYA
                     VLGGGKRVRALLSYAAAELAGATPTAADAGAVAVELIHAFSLVHDDMPCMDDDDLRRG
                     KPSCHKQYDDATALLVGDALQSLAFEYIADTHATQALKQVQVLAKATGSRGMCGGQSI
                     DLQSTGKLLSRSELETMHQYKTGALIQAAVLLGAYAADNTDQQLIHALEQYSQHMGLA
                     FQVVDDILDATADTQTLGKTAGKDALQEKSTYVSLLGLEEAKALVTTLHAQSLQLLAG
                     FDSKADPLRGIAKFICERSF"
     gene            2770..4611
                     /gene="dxs"
                     /locus_tag="VNZ84_08395"
     CDS             2770..4611
                     /gene="dxs"
                     /locus_tag="VNZ84_08395"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HWU67016.1"
                     /translation="MTSLLSRIQSPTDLRKLERAQLKVLAAELRAFLIHSVSQTGGHL
                     SSNLGVVELTIALHYIYNTPDDRLVWDVGHQTYPHKILTGRREAMANLRKAGGIAGFP
                     KRDESEYDTFGTGHSSTSISAALGMAVAAKAQGSNRRSVAIIGDGAMTAGMAFEALNN
                     AGDMDTNILVILNDNDMSISNNVGALNNYLARLMSGKLYDSVRRSGKKVLDIIPPVKE
                     FARRAEEHAKGMVTPGTLFEEFGFNYIGPIDGHDIDTLVDTLSNIRQLQGPQFLHVVT
                     QKGKGYSPAEDNPNKYHGVSKFDPQNGHSLSHSERPTYTQIFGDWLVDMAAADHKLVG
                     ITPAMCDGSGLNRFAEAYPQRYFDVGIAEQHALTFAAGMACDGLKPVVAIYSTFLQRA
                     YDQLIHDIALQDLDVLLAIDRAGLVGADGPTHAGSFDLSFLRCIPNVVIMAPADENEC
                     RQMLSTGYHYPGTAAVRYPRGSGIGAAVTPTLETLEIGKAELKRQGKKVAILAFGSML
                     QPSLKAAESVDATVVNMRFIKPLDVSMVLEMARTHDYLLTVEENALMGGAGAAVMECL
                     QAQGISKPLRCLGLPDQFIEHGSHETMLSECGLDKDGIISAIEKLIT"
     gene            4664..5470
                     /gene="folE2"
                     /locus_tag="VNZ84_08400"
     CDS             4664..5470
                     /gene="folE2"
                     /locus_tag="VNZ84_08400"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HWU67017.1"
                     /translation="MNQPVIPTTIEDVQNTPDVRRLDINKVGIKSIRHPVIVKDKTGG
                     EQHTVAMFDMYVHLPHNFKGTHMSRFVEILNMNEHAISIDSFETILRQMVERLEAESG
                     YVEMRFPYFINKAAPVSGVKSLLDYEVTFVGEIKDGKFEITVQVMVPVTSLCPCSKKI
                     SDYGAHNQRSHVTVTALLNEFMWLEDLITLIEDQASCQLYGLLKRPDEKFVTERAYDN
                     PKFVEDMVRDVAAQLNDEKRIVKYVVESENFESIHNHSAYALIERDKRVQ"
     gene            complement(5662..6834)
                     /locus_tag="VNZ84_08405"
     CDS             complement(5662..6834)
                     /locus_tag="VNZ84_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU67018.1"
                     /translation="MSHALHFNLSRFYFIYYFFVGAFVPYWGLYLTSESFSPADIGIL
                     MSLFQLSRIIAPNLWGWLADHTRQRVRWIRTTAFLGLLGFVAIFWAHDFTTMFVVMVA
                     LSLFTSSTLPLSESLVLAHLASTNGHYSKIRLWGSWGFIVASILLGYLTDWFGIGSLL
                     WFILLVQCTLFGLTWKLPEAHVPPHAHDDFSVWQILKQPAVMSLLIGCAMMVTAHGLM
                     YNFYSIYLAEHGYSKGMIGWLWSLGVICEIGIFMKMPWIMTHFKLKTILLASLLIGVV
                     RFAMMGLVPDQLILLLLAQTMHAFTFGSFHAASVEVVTQYFNGRHQAKGQAIYNSVAY
                     GVGGALGGLLGGFALQYLGGQLTFTLAALFPLLGAIIVALGLHLSQPYARNGMFSH"
     gene            complement(6895..>7834)
                     /gene="aroC"
                     /locus_tag="VNZ84_08410"
     CDS             complement(6895..>7834)
                     /gene="aroC"
                     /locus_tag="VNZ84_08410"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWU67019.1"
                     /translation="TSRHVTQRAEADEVEILSGVFEGKTTGTPIGLLIRNTDQRSQDY
                     SKIMETFRPGHADYTYTQKYGIRDYRGGGRSSARETAVRVAAGAIAKKWLKQQYGVQI
                     RGYLSQIGEIKVPFVSWEALSQEGNAFFSPNIEILPQLEAYMDQIRSERDSVGAQITV
                     VAEKVPVGLGEPVFDRLDADIAHAMMGINAVKGVEIGAGFDSIAQRGSVHSDELTPEG
                     FATNHAGGILGGISSGQDIVVNVAFKPTSSIPQQRHSINQAGEAVMMQTTGRHDPCVG
                     IRATPIVEAMLALVLMDHALRNRGQNADVNSSVPRL"
BASE COUNT         2141 a         2008 c         1867 g         1818 t
ORIGIN      
        1 attgaattga tgatcgtggt tgcgattatt ggtattttgg cttctgtggc gattccagca
       61 tatcaggatt acacaaaaga cgccgctgac aacgcatgtg cagtacaaac aaaaggattc
      121 actaataatt atgttctagc acaacaaact aacagaacac ttccaaccag tactgctggt
      181 gcttgtaggg atgttgcaac aattactgcc acacaaattt cagcacaagc caaagctcca
      241 ggcgacaaaa cgactacttg catcattgca actggtgttt gcagctaatc agcaatatta
      301 atcgcactcg gtaaaaaccc caatcaacta gttggggttt ttaccttggt cccgtagtct
      361 taactaaaac actttagtga tggacaccta tgcgcaattt tcaaaaaggc tttactctta
      421 tagagttaat gattgtagta gcaatcattg gtattttagc ttctatcggc attccccttt
      481 atcaggacta cactaaggat gcagccaata atgcttgtgc tgttcaaact aaacagtttg
      541 tcgataacta caacttggct agtcaaacta acaagtcact ccctaccagc attgcaggcg
      601 catgtgcaga tgcagctatt attacaagta ctagtattac agctacggca aaatacccag
      661 gcagcaaaac gaccacttgc acgattgcca ctggtgcctg tataacttct taaactgata
      721 ctctatttag cctgaaaata tagtcgaggt gtgaggtctt actgaactct gagagtttga
      781 taaaacagaa taatcgccct ctctagggtg atgaattttc tgtcgcactc ttatactaaa
      841 tacccaatta acaataataa aaagctacat gaaacaccat gaaagaagcc ataataatcg
      901 actcaaaaaa gcatttagca accgtctttt ggttacacgg cctcggcgcc gacggccatg
      961 actttgagcc tgtcgtgcaa atgctcaacc tgccgcatat taagtttgtt ctgccacatg
     1021 ccccttatcg gccagtcacc ttgaacaacg gttacgaaat gcgtgcctgg tatgacattt
     1081 ttgggctgca atcagaaagc cagcaagacg aagttggcat cactcaaatg caggcaacca
     1141 tcaactctat gattgcagca gaaattgccc ttagcattcc ttcgcaacgc attttgctgg
     1201 caggcttctc gcaaggtggc gccatagcct tgcacacagc cacgcgcttt gatcaacccc
     1261 tagctggggt attagcactc tcgacttatt tacctctaaa gcatcaactc aaagaaaaac
     1321 agcatccagc caaccagcaa ttaccgatct ggatggcaca tggacgtcaa gacagtgtca
     1381 tcactctctc aacagcggca tcatccagac aaacactgga agccgcagaa tataaagtgg
     1441 agtggcatga gtacgatatg ccacatagcg tttgtgaagc cgaaattaac gatatccgcg
     1501 aattcctgct gcgtgtttta cccgcctaag ccagcttctc acttgataat aatccctcaa
     1561 gttaggggag ttttgttaaa atagctggcg ttttatcagg ccaattttca tgagtgcttc
     1621 caaaaagtca ccgtctgcgg tgagtgatca acaaaccttt gccgaacgac tggctgcgct
     1681 ggataacatt gtcaggagca tggagtctgg agagcttccg ctagaggctg cattaaccgc
     1741 ctacacagaa ggtatgcagt tgctgcaggc ctgtcaggag tccttacagc aagctgagca
     1801 gaccgtcatg atattgaacc agcaacaact cacccctttc gaacctaatt aacgcaccct
     1861 ccttcgcctt ttactcatgt ctacccctgc atttgttttc gatcactggg cagcagcaca
     1921 tcagcatcgt gttgaagaag tgcttgtgcg tacattgcca gttaacgcca tactgccaga
     1981 acgtttgcat gaagcgatgc gctatgctgt gctgggtggt ggcaagcgtg taagggcatt
     2041 attaagttat gccgcagcag aactggctgg ggcaacacca accgccgccg atgcaggcgc
     2101 agttgcggtg gagttgatcc atgcgttttc cctggtgcac gatgatatgc catgcatgga
     2161 tgatgatgat ttacgtcgcg gcaaacccag ttgtcacaaa caatatgatg atgcgaccgc
     2221 cttactggtc ggtgatgccc tgcaaagcct ggcgtttgaa tatattgctg atacccacgc
     2281 cacgcaagcg ctcaaacagg tacaagtatt ggcaaaagct actggttcac gcggtatgtg
     2341 tggtggccag agcatagatt tgcaaagtac cggcaaactg ctttcccgca gtgagttgga
     2401 aactatgcat caatacaaaa ccggcgcgct tattcaggct gcagtattac tcggtgctta
     2461 tgccgctgac aatacagatc agcaacttat ccatgccttg gaacagtata gccagcatat
     2521 ggggctggca tttcaagtcg tggatgacat tctggatgcc accgcagaca cgcaaacgct
     2581 gggaaaaacc gctggcaaag acgccttgca ggaaaaatct acttacgtca gcttactcgg
     2641 cctagaggaa gccaaggcac tcgtgaccac tttgcatgca caaagcttgc agttgcttgc
     2701 gggttttgac agcaaggccg atccacttag aggcattgcc aaatttattt gtgagcgctc
     2761 attctgatca tgacttcgct gctgtcccgc atacaatcgc ccacagattt acgaaaactt
     2821 gagcgcgccc agctcaaagt gctggcagct gagttacgcg ctttcctgat tcacagcgtg
     2881 tcacaaactg gcggccactt atcctccaac ctgggcgtgg tggagctcac catcgccctg
     2941 cactacattt acaacacacc ggatgaccgt ttggtatggg atgtcgggca tcaaacctat
     3001 ccgcataaaa tcctgaccgg ccgccgtgaa gcgatggcta atttgcgcaa agcaggcggc
     3061 attgccggat tccccaagcg tgatgaaagc gaatacgaca cctttggtac cggccactca
     3121 agcacttcga tttcagcagc tttaggcatg gccgtagccg ccaaggcgca aggctccaac
     3181 cgccgtagtg tagccattat tggtgatggc gcgatgacgg caggcatggc atttgaagcc
     3241 ttgaacaatg ctggcgacat ggacaccaat attctggtga tcctcaacga caacgacatg
     3301 agcatctcca acaatgtggg cgcgctcaac aattacctgg cccgcttaat gtcaggcaaa
     3361 ctgtatgaca gcgtacgccg cagtggcaaa aaagtgctgg atattattcc gcctgtgaag
     3421 gaatttgcac gccgtgccga agagcatgcc aagggcatgg tcacgccagg cacgctgttt
     3481 gaagagttcg ggtttaacta tatcggcccg attgatggcc atgacatcga tacgctggta
     3541 gataccttga gtaatatccg ccagttgcaa ggcccacagt tcttgcatgt ggtgacacaa
     3601 aaaggcaaag gttacagtcc ggcagaagac aaccccaaca aataccatgg tgtcagcaag
     3661 tttgacccac aaaatggtca cagcctgagc catagtgagc gcccgaccta tacacagatt
     3721 tttggtgatt ggctggtcga catggcagca gcggatcata aactggtcgg catcacgccc
     3781 gccatgtgtg atggctctgg cctcaaccgc ttcgctgaag cgtacccgca acgctatttc
     3841 gatgttggca tcgcagaaca acacgcgctg acatttgccg ctgggatggc ctgtgacggc
     3901 ctaaaacctg tggtggcgat ttactccaca tttttgcaac gcgcctatga ccaactgatt
     3961 cacgacatcg cattacagga tttggatgta ttgctggcga ttgaccgagc aggattggtc
     4021 ggtgccgatg gcccaaccca tgcaggcagc tttgatttaa gctttttgcg ctgcatcccg
     4081 aatgtcgtga ttatggcgcc tgcagatgaa aatgaatgcc gccagatgct cagcaccggc
     4141 taccactatc caggcactgc agccgtgcgc tacccccgcg gaagcggcat cggtgccgcc
     4201 gttactccaa cgctggagac cctggagatt ggcaaagccg aactcaagcg ccaaggtaaa
     4261 aaagtggcga tcctggcatt tggcagcatg ctgcaaccat cactaaaagc agcagaatcc
     4321 gtcgacgcga cggtagtgaa tatgcgcttc atcaaaccac tggatgtgtc catggtactg
     4381 gaaatggcgc gcacgcatga ttacctgctg actgtagaag aaaatgccct tatgggtggt
     4441 gcaggggccg cagtgatgga gtgcctgcaa gcgcagggca tcagcaaacc cttgcgttgc
     4501 ttagggttac cagaccagtt tattgagcat ggcagccacg aaactatgct ctctgaatgt
     4561 ggtctagaca aagacggaat tatttcagct attgagaaat taataaccta gtagtaaact
     4621 caactattaa ttaacaggcg gaaaagcccg ctatggaacc ctgatgaatc aacccgtgat
     4681 ccccacaacc attgaagatg tccagaatac gccggatgtg cgacgcttgg acatcaacaa
     4741 agtcggtatc aagtcgattc gccaccctgt gattgtgaaa gacaaaactg gtggtgaaca
     4801 gcatactgtt gccatgtttg atatgtatgt gcatttgcca cacaacttca aaggtacgca
     4861 catgtctcgc ttcgtcgaga tactgaacat gaacgagcac gcgatttcga tagactcatt
     4921 cgaaaccatt ttgcgccaaa tggtagagcg gctcgaagct gagtctggtt atgtggaaat
     4981 gcgtttccct tactttatca acaaggcagc acccgtttcc ggtgtcaaaa gcctgcttga
     5041 ttacgaagtc acctttgttg gtgaaatcaa ggacggcaaa tttgaaatca ctgtgcaggt
     5101 catggtgcca gtgaccagct tgtgcccatg tagcaagaaa atctcagatt acggcgcgca
     5161 caaccagcgc tcgcacgtca cagtcaccgc cctgctcaat gagtttatgt ggctggaaga
     5221 cctgatcaca ctgattgaag accaggcttc ttgccagctg tatggtttgc tcaaacgccc
     5281 ggacgagaag tttgtcactg aacgcgctta tgacaatccg aagtttgttg aagacatggt
     5341 gcgtgatgtg gcggctcagc tcaatgatga aaaacgcatt gtgaaatatg tggttgaatc
     5401 tgaaaacttt gagagtattc acaaccatag tgcatatgcc ttgatcgaga gagacaaacg
     5461 cgttcagtaa tctgttttga caaagtttca gtgtaggcta acatcgattt cgatgttagc
     5521 gaagcggccg aaactaaaac ttcgagccca tccacaatca tagcgcctat gcgctgattg
     5581 aacgcgataa gcgcgtcagc taatcacgac gcatcgtata aaaacagccc gttcacgggc
     5641 tgtttttatt tctgcagcgt attaatgact aaacatgccg ttacgggcat aaggctgcga
     5701 caaatgtaac cccaaagcca caatgatggc gcctagcagc gggaataaag cggccaaagt
     5761 aaaggttagt tgtccaccca aatattgcaa ggcaaagccg cctagcaagc cacctaaagc
     5821 gccacccaca ccataagcaa cactgttata aatcgcttgt cctttggcct gatggcgacc
     5881 attaaagtat tgcgtcacaa cctctaccga agcggcatgg aagctgccaa aagtgaaggc
     5941 gtgcatggtt tgcgctagca acagtagtat taactgatct ggtaccaggc ccatcatggc
     6001 aaaacggacc acgcctatca gcaggctggc cagtaaaata gttttgagtt tgaagtgcgt
     6061 catgatccat ggcattttca taaaaatacc aatctcgcaa atcacgccta gcgaccacaa
     6121 ccaacctatc atgcctttgc tgtagccgtg ctcagccagg taaatcgagt agaagttata
     6181 catgaggcca tgcgccgtca ccatcatagc gcagcctatc agcagtgaca tcacagcagg
     6241 ttgcttgaga atctgccaaa cagaaaagtc gtcatgcgca tgtggcggca cgtgggcttc
     6301 cggcagtttc caggtcaggc caaacagggt gcattgcacc aacaggataa accagagcaa
     6361 gctaccaatg ccaaaccagt ccgtcagata gcctagtaat atcgaagcca cgataaaccc
     6421 ccatgaaccc cacaaccgga ttttgctgta atggccattg gtcgaggcca gatgcgcgag
     6481 taccaatgat tcagacaagg gtaaggtcga gctggtaaac aagctcaggg ccaccatgac
     6541 cacaaacatg gtagtgaaat catgtgccca gaaaattgca acaaacccca gcagtcccag
     6601 aaaggctgtc gtgcgaatcc agcgcacacg ttgccgggta tggtcagcca accagcccca
     6661 aaggttaggt gcaataattc ggctaagctg gaacagtgac atcaggatac caatgtctgc
     6721 cggagagaag gattctgatg tcaggtataa gccccaataa ggaacaaaag caccgacgaa
     6781 aaaatagtaa ataaaataga aacgcgagag attaaagtgt aaagcgtgac tcatgacata
     6841 ctcgcgtttg tgtgaatatc gctcaagacc atgacatgag cagaaaagag tttattacaa
     6901 cctgggaacg ctggaattca cgtctgcatt ctggccacgg ttgcgcaagg catgatccat
     6961 cagcaccaat gccagcatag cctctacaat cggtgtcgcg cggataccta cacacggatc
     7021 atgacggcca gtagtttgca tcatgacagc ctcgcctgcc tgattaatgg agtgacgctg
     7081 ttgcgggatg ctcgaagtcg gcttgaaggc cacattgacg acaatatcct gcccgcttga
     7141 aatcccaccc aaaatgccac cggcatgatt ggtcgcaaaa ccttcaggcg tcagctcgtc
     7201 actatggaca ctgccacgct gtgcaatgga atcaaatccg gcgccaatct ctaccccttt
     7261 cacggcatta atgcccatca tggcatgcgc aatatcggca tccagtcggt caaatactgg
     7321 ctctccgagg ccgacgggga ctttttctgc cacgaccgtt atctgcgctc ccacagaatc
     7381 acgctcactg cggatctggt ccatataagc ttccagttgc ggcaggattt cgatattcgg
     7441 agaaaagaaa gcattgccct cttgcgataa cgcttcccaa ctcacaaatg gaaccttgat
     7501 ctcgccaatc tggcttaaat acccacgaat ttgtacacca tactgctgct tgagccattt
     7561 tttggcaatc gcgcctgcgg ccacccgcac ggcggtctca cgcgcactgg aacggccacc
     7621 gccacgatag tcacggatgc catacttttg tgtgtaggta tagtcggcat ggccgggacg
     7681 gaaggtctcc atgatcttgc tgtaatcctg cgaacgctgg tccgtattac ggatgagcaa
     7741 gcctatcggg gtgccggtgg ttttaccttc aaagacccct gacagaattt caacttcatc
     7801 tgcctcagcg cgctgtgtga catgccggga agtc
//
LOCUS       DATMSK010000254        19790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_349701,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000254 DATMSK010000000
VERSION     DATMSK010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 19790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19790
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349701"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VNZ84_08415"
     CDS             complement(<1..492)
                     /locus_tag="VNZ84_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWU67020.1"
                     /translation="MRLDDLEESQHVEDSRGRSVLGGRSIGIGTIAIALVAMYFGVDP
                     NLVLNIAQQAQPAAESQAVPADSSDPKVKFVSRVLWSTEQVWQKAFADAGQQYTAPNL
                     EIFGGATQTACGQGQAAMGPFYCPADQKVYIDLSFYQELQERFKAPGDFAQAYVIAHE
                     VGHH"
     gene            573..1166
                     /locus_tag="VNZ84_08420"
     CDS             573..1166
                     /locus_tag="VNZ84_08420"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWU67021.1"
                     /translation="MFTGIIQTVGRIAEVVPHGEDCALRIEAPALGMEDVQLGDSIAV
                     NGVCLTVTDFNSSSFQVMVSKVTLDVTTGLGQAGPVNLEKALRLADRLGGHLVTGHVD
                     GIGTITALQEVGECWLLKIRAPHAISKYIAKKGSLCVNGISLTVNEIDQDEVSINLIP
                     HTMLHTVMQYAKAGDPVNLEIDLIARYVERMQAWEQE"
     gene            1166..2275
                     /gene="ribBA"
                     /locus_tag="VNZ84_08425"
     CDS             1166..2275
                     /gene="ribBA"
                     /locus_tag="VNZ84_08425"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWU67022.1"
                     /translation="MSAQISPIEAIVADIRAGKMVILVDDEHRENEGDFVIAAEFVTA
                     EHINFMAKYGRGLICLTLTEQRCRQLDLPPMVQKNGTRLGTNFTVSIEAAEGVTTGIS
                     AADRATTIRAAIAKQANAGSIVSPGHIFPLSAMNGGVLVRAGHTEAGCDLAQLAGCEP
                     ASVICEILKDDGEMARLPDLMQVAAEHDIKIGTIADLIHYRAAHETLIERNAERNIET
                     VFGPMRLIAYRDNIAQETHLVLIKGDPQTSDEALVRVHEPLSMIDLLDSSHQAHSWNL
                     LHAMRTISTAPCGVIVLINHDEDGLGLTDRILRADQKMVVNQELRNYGIGSQILIDLG
                     VKKMRLMAAPRKMPSMDGFGLEITGYLSAEEASPI"
     gene            2272..2877
                     /locus_tag="VNZ84_08430"
     CDS             2272..2877
                     /locus_tag="VNZ84_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HWU67023.1"
                     /translation="MSEQLTLNNDEARLNLTGQILIAMPAMEDPYFSKSVILVCNHDQ
                     DGAMGMILNHPLQLNVGDLFEQLQMNCDAPHQEERQVHFGGPVQVERGFVLHTPATEF
                     NTTLDLSEGLAMTSSKDILEAAARDEAPPEMFIALGYTGWSAGQLEEEIQSNTWLTLP
                     LADTQQLHKLIFKLPNDDKLSWAMQQLGVDFATLSEVAGHA"
     gene            2870..3373
                     /gene="ruvX"
                     /locus_tag="VNZ84_08435"
     CDS             2870..3373
                     /gene="ruvX"
                     /locus_tag="VNZ84_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWU67024.1"
                     /translation="MPDAVKHGKQPLNQSHLPITQRRYPEISGSVIAFDFGEKRIGVA
                     IGDTLLKLAHPLLTIEAEENAAKFAQIEALLNEWQPALLIVGLPMSLEGEAHAMTALA
                     QKFAQRLEGRFNLPVMMVDERLSSAEAAQSLREAGIKGRAQKPLLDQVAAQTILQSYF
                     DAYHGRV"
     gene            3386..3943
                     /gene="pyrR"
                     /locus_tag="VNZ84_08440"
     CDS             3386..3943
                     /gene="pyrR"
                     /locus_tag="VNZ84_08440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HWU67025.1"
                     /translation="MTLPDPEQLLDHLYQQIKPLIQEDTALVGIYTGGVWLMERLLKR
                     LQTELGHDILFGKLDAAMYRDDYAQRGLKTTAHPGYIPFDVNGKHIILIDDIFYTGRT
                     TRAVMNELFDYGRPASVKLATLINRGGAQLPIRPDMVGADLPLQAHQSFDLAMNTLGR
                     LSLSVSESDPSATKPSANSGTDAHV"
     gene            3936..4892
                     /locus_tag="VNZ84_08445"
     CDS             3936..4892
                     /locus_tag="VNZ84_08445"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWU67026.1"
                     /translation="MYNPQLNAQGQLQHLLSIEGLPKRILTDILDTAENFVGVAEREV
                     KKVPLLRGKTVCNIFFENSTRTRTTFEIAAKRLSADVLNLNVSTSSQSKGETILDTVN
                     NLIAMHADMFVVRHQQSGAAHFIAQHVPPHISVINAGDGRHAHPTQGLLDVFTIRKYK
                     PEMHNLRVAIVGDVLHSRVARSEIHALTTLGVPEIRVIAPRTLLPTDVEKLGVQVFHN
                     MEQGLKDVDVVMMLRLQNERMSGALLPSSQEFFQTFGLTPERLALAKPDAIVMHPGPM
                     NRGVEIDSAVADGKQSVILPQVTYGIAVRMAVMAILGGGNAA"
     gene            4889..6187
                     /locus_tag="VNZ84_08450"
     CDS             4889..6187
                     /locus_tag="VNZ84_08450"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWU67027.1"
                     /translation="MNIVIKQGRLMDASQSLDQVKDLYISAGKIVAIGEAPAGFKADQ
                     TLDASGKWVLPGLVDLCARLREPGSEYKATLLSELRAAMAGGVTSLACPPDTDPVLDE
                     PGLVEMLKHRAKSQALAHVYPLGAVTRQLEGQLLTEMYALTAAGCVGFSQADTAIVDT
                     QVLWRAFEYAASFGYTLFMRAEDPYLAKNGVAQDGEVASRLGLKGIPSAAESIALQTM
                     LRIAQATGVRLHIARVSTAESVELIRQAKQSGMQVTADVSIQHLHLTDMDIGFFDGMV
                     NLKPPVRTQRDRDALRAGLVDGTLDAVCSDHTPVDDDAKALPFAEAHPGASALELLLP
                     LTLKWAAQAKLAPVQALQKITQIPADILGIKAGNLQLNSIADVVIYDPEMEWRVDGRV
                     LVSQGKNTPFVHHALTGKVTATLTQGNLVFQQETLKLAHH"
     gene            complement(6264..7424)
                     /locus_tag="VNZ84_08455"
     CDS             complement(6264..7424)
                     /locus_tag="VNZ84_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWU67028.1"
                     /translation="MDGTQTVGDHAQTEKFVNDLLKLMITRKASDLFITAGFPPAMKI
                     DGKLTPVSSQTLNTQQTAEIARTVMNDKQAQEFKETLECNFAISMSSVGRFRVNAFIQ
                     RSAVGLVFRVINTNIPKFDDLNLPPILKDVVMAKRGLIIFVGGTGSGKSTSLAAMLGH
                     RNESSYGHIITIEDPVEFIHGHRNCIVTQREVGVDTESWHAALKNTLRQAPDVIMIGE
                     IRDRETMDYAIAFSETGHLCLATLHANSTNQALDRIINFFPEERRQQLLMDLSLNLRC
                     IASQRLIPKLDSTGRVAAVEVLLHSPLISDLIYKGDVHAIKEIMAKSRELGMQTFDQA
                     LFDLFESGQISYDDALRNADSVNDLRLKFKLNSKVMNPENQERSQISNLDIL"
     gene            complement(7429..8472)
                     /locus_tag="VNZ84_08460"
     CDS             complement(7429..8472)
                     /locus_tag="VNZ84_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWU67029.1"
                     /translation="MDISDLLAFSVKNKASDLHLSSGLPPMIRVHGDVRKINLPAMEH
                     AEVHRMLYDIMNDSQRKVYEETLECDFSFEIPDLARFRVNAFNQQRGAGAVFRTIPSK
                     VLTLDELNAPKVFKDIADTPRGIVLVTGPTGSGKSTTLAAMIDYINETQMSHVLTVED
                     PIEFVHTSKKSLINQREVGPHTQSFENALRSALREDPDVILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTIDRVVDVFPAAEKEMVRSMLSESLRAVISQTLCKTKDEQG
                     RVAAHEIMMGTPAIRNLIRENKIAQMYSAIQTGQSMGMQTLDQNLQDLVKRNVISAAE
                     ARTKAANRDTIIG"
     gene            8570..9286
                     /locus_tag="VNZ84_08465"
     CDS             8570..9286
                     /locus_tag="VNZ84_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWU67030.1"
                     /translation="MTAISDNLALIHQQIADARAHFNAPQNVTLCAVSKAQPAEAIRV
                     AYEAGQTVFGENYLQEALAKQEKLQDCAIAWHFIGPIQSNKTQPIARQFAWVHSVDRL
                     KIAQRLSDARPLELAPLNICLQVNISEEASKSGASGQELLELALNIKQLPRLQLRGLM
                     AIPAPCSDIEQQRDQFRQVRALFEQLNNHGLQLDTLSIGMSGDFAAAIAEGATLVRIG
                     TAIFGARTKPFSKDSVNQGT"
     gene            9292..10125
                     /gene="proC"
                     /locus_tag="VNZ84_08470"
     CDS             9292..10125
                     /gene="proC"
                     /locus_tag="VNZ84_08470"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWU67031.1"
                     /translation="MQSFSAARIAFIGGGNMAQALMIGLQQRAFAMQNITVIDPDTAK
                     HEHLQATLGVNTSASLTTGSLAVDVVVLAVKPQQLHSVAQALAPLLQSQLVVSVAAGI
                     RTADLSRWLNGYTTIIRTMPNTPAQIQSGITGAYALPAVTQAQREIADAMLQAAGEVV
                     WLTDEDQLDAVTAISGSGPAYVFLMIEALAAAGLSLGLSEAQSLQLSLATFKGASLLA
                     AGSTTPVATLREQVTSKGGTTEQGLLSMRQHDIHGMMQHAAEQAARRAKELGDQLGAQ
                     A"
     gene            10122..10673
                     /locus_tag="VNZ84_08475"
     CDS             10122..10673
                     /locus_tag="VNZ84_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWU67032.1"
                     /translation="MMLQNMTTFLLNAVFGILTFLLVLRFLMQWTRTSFQNPLGQMTM
                     ALTDFMVKPARKIIRPARQWDMSTLLLALLMQIVLFALLTLLAGAPASPFFWIWQAIF
                     GVLGQIVDVFFYAILLMAILSWVNPYSSIYGVLNQLSSPILDPLRRVLPQIQGFDFSA
                     LVALILLQMISHIVLPGLAAGIL"
     gene            complement(10706..12172)
                     /gene="rng"
                     /locus_tag="VNZ84_08480"
     CDS             complement(10706..12172)
                     /gene="rng"
                     /locus_tag="VNZ84_08480"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HWU67033.1"
                     /translation="MAQEVLINVTPQETRVAILEQGVVQELHIERASSLGIVGNIYRG
                     TVVRVLPGMQSAFVEIGLPRAAFLHAADIMECHAEEGDIKKERPIQEVLHEGQSIIVQ
                     VIKEAIGTKGARLTTEISIAGRFLVYLPYQQHIGVSQRIDIEEEREFLRNRLLGLLPE
                     EREGGYIIRTMSENATDEELLADIEYLRKVWSKIQAESTKVGERSLIFYDLSLPRRIL
                     RDVVCTETTSIRVDSREMFERLKEFAELYVSHAVKPLTHYKGERSLFEFYDIENEIEK
                     ALARKVELKSGGYLIFDQTEALTTVDVNTGSFVSGKNLSDTIFKTNLEAAQAIARQLR
                     LRNLGGIIIIDFIDMDEDAQREAVLEELRKAVMLDRARINVNGFSALGLVELTRKRTR
                     ESLAHLLCESCQVCQGRGELKTAQTICYEIMRELLRESKQFRNAKEFKILASPEVINM
                     LGDEESQSLANLSDFIKKPLSLHPDTQYGREEFDIVWM"
     gene            complement(12220..12786)
                     /locus_tag="VNZ84_08485"
     CDS             complement(12220..12786)
                     /locus_tag="VNZ84_08485"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWU67034.1"
                     /translation="MQQRNIILASRSPRRVELLAQLGVQCEIAPADIDESSVPGEDPA
                     HYVLRLAQEKALAVAATHGERGLAILAADTTVALGRDILGKPADAREAMAMLSRLSGS
                     IHMVHTAVAVYQAGQLRYLLNSTKVEMMQIPVTVLEAYVASGEPMDKAGAYGIQGRAG
                     AWIARIDGSYSGVMGLPLHETAQLLHAW"
     gene            12952..13809
                     /locus_tag="VNZ84_08490"
     CDS             12952..13809
                     /locus_tag="VNZ84_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU67035.1"
                     /translation="MNSLEHLLQRAEQLITRLETLLPAPPAETNWQAVAWRWVKHGAQ
                     GHLQVVAHPHAVLLEDIQFIDRQKTEVVRNTRQFLQGLPANHVLLTGARGTGKSSLVK
                     ALLNSYAEQGLRLIEVEKQDLNDLPQIAQLIRLRPEKFIIFCDDLTFEANDIGYKALK
                     VILDGSIEHASPNLLVYATSNRKNLMPEYMADNQPIIDKGEIRQNDTIDEKTSLAERF
                     GLWLSFYSFDQDEYLSIAEHWLKTFGIAMDEAAHHAALQFYHTRGARSGRVAYQFAKD
                     YAGQLQLKS"
     gene            13815..14774
                     /locus_tag="VNZ84_08495"
     CDS             13815..14774
                     /locus_tag="VNZ84_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HWU67036.1"
                     /translation="MTKLVQVAVAILMQPNGEYLLANRPSGKGWAGWWEFPGGKIEAC
                     ETPEHALIRESQEELGITPTQIQPWIKRRYDYPATHDAEAKTVLLHFFFVYAWQGELQ
                     AREGQEFAWQHPQKLNVTPVLPANAPIMQALSMPPVYAISNVSEMVVPAFLQALKQQL
                     EQGLQLLQIREPQLNDTAIAKLSEQVLQLCAHYDCRCLLNGSPEQALQLGYHGVHLNS
                     QRLMALTQKPDNLLVGASCHDVEQLQQAQALQLDFALLSPVLPTKSHPEAPGLGWEKF
                     AEMLSGLEVPVYALGGMHLEHLSQAQSCGARGIAMQRAIWSTT"
     gene            14839..15069
                     /locus_tag="VNZ84_08500"
     CDS             14839..15069
                     /locus_tag="VNZ84_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HWU67037.1"
                     /translation="MQPPQPNNPLHGVTLEYMVEQLVEHYGWDQLAQKVNINCFKNDP
                     SVKSSLKFLRRTSWARAEVERLYLETFAQNHG"
     gene            complement(15109..15552)
                     /locus_tag="VNZ84_08505"
     CDS             complement(15109..15552)
                     /locus_tag="VNZ84_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HWU67038.1"
                     /translation="MHKTAFFLRLVASLCLLGFLGIAHAEVKLTDAWVRASNPGQSVG
                     AAYVTLSSTEDVTLVYAETERAGTVEMHSMTMQNGIMKMRSMEELPVPAGKPVKLAPG
                     GLHLMLFELATPFKAGEQVKFRLCFKDKQGKITDQFVTMPVKSAP"
     gene            complement(15574..15765)
                     /locus_tag="VNZ84_08510"
     CDS             complement(15574..15765)
                     /locus_tag="VNZ84_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HWU67039.1"
                     /translation="MTQPAVKARKVACPSCGETTEYSPANPFRPFCSERCKLVDLGDW
                     ATEKFRIPDSTAPDLYEPE"
     gene            complement(15762..16535)
                     /gene="zapD"
                     /locus_tag="VNZ84_08515"
     CDS             complement(15762..16535)
                     /gene="zapD"
                     /locus_tag="VNZ84_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HWU67040.1"
                     /translation="MSSYEFPFNERIRTYLRLEDLFIKMLHHIEIGHEISHHVALISM
                     LQVLDLIDRGDLKVDLLQELDRHKVQLSFLQNNPNIDQQALASTLSGLDRCATALRSD
                     HQKLGQSLRENDWLMSVKQRTSIPGGVCEFDLPSYRHWLYLGVERRKKDFDHWLTKLM
                     PMYEAIRLILQLLRGSGQVLSLVAHHGAYQQQLSGHKPAQLLRIEIDHDECFPEVSAN
                     KYAINIRFQKLDFVQRPKGCDQDISFKMIICNFTGAGHN"
     gene            complement(16605..17207)
                     /gene="coaE"
                     /locus_tag="VNZ84_08520"
     CDS             complement(16605..17207)
                     /gene="coaE"
                     /locus_tag="VNZ84_08520"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881719.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWU67041.1"
                     /translation="MSKVVAVTGGIGSGKTEVCKYFSALGVPVVDLDQIAHEMSMPGS
                     PAMQAVRTTFGDEMFEIDGRLNRAKLRELVFAEPDALEQLNQIMHPAIRAEAVRQIAG
                     YSAQAYVVLAIPLLVESREDWQMIDHVLVVDCDEQTQLARIVQRSQLSETMAQAMIAA
                     QSSREERLSIADSVIVNEQTLDNLQEKVLEFHKNFSKTCQ"
     gene            complement(17232..18104)
                     /locus_tag="VNZ84_08525"
     CDS             complement(17232..18104)
                     /locus_tag="VNZ84_08525"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HWU67042.1"
                     /translation="MSEILEPVLSLLRTQPHFLQTVCLVLGLLVGSFLNVVIHRLPKM
                     MEREWHASCLDLQGQTAPEQPKYNLVVPRSACPHCGHQITALENIPVISYLFLRGRCK
                     GCKTPISARYPLVELLTGLLSLAVGVQYGYGMLTLFALVFVFALVALTFIDFDTQLLP
                     DDITLPLLWLGLLLNIKYGFTDLTSAVIGAMAGYLVLWSVYWLFKLVTGKEGMGYGDF
                     KLLAAIGAWFGWQLLPAVILLSSVVGSVIGIGLILLKGKTRQTAIPFGPFLALGGIAA
                     LFYGQALASYYLVP"
     gene            18303..18701
                     /locus_tag="VNZ84_08530"
     CDS             18303..18701
                     /locus_tag="VNZ84_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67043.1"
                     /translation="MSDQIKFTPPTEVELNQVEHPAWDEPVICRVEVDLPGWLTKLTG
                     QAHWEVYSEEEEENCVSFAMRSSHARHLHKATTPIRDDMQAEVTLYHNGYAVVDVDGK
                     SLFDGALTNATNDCARLSYYHMSGEPIALN"
     gene            complement(18744..19673)
                     /locus_tag="VNZ84_08535"
     CDS             complement(18744..19673)
                     /locus_tag="VNZ84_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWU67044.1"
                     /translation="MQQVDAVIRQSLSSQVPLINQISEYIIHSGGKRLRPALVLMSGD
                     LAGAVTSEHHSLAAIIEFIHTATLLHDDVVDASELRRGKSTANHVYGNPASVLVGDFL
                     YSRSFQMMVKLQNMRVMEILADATNVISEGEVLQLLNVRNIGVSEQDYLQVIHFKTAK
                     LFEAATRLGAVQCQVSAEQEQGLATYGMHLGTAFQLIDDVLDLTGESEKIGKTLGNDL
                     AEGKVTLPMLYAIQQAPAAQADLVKQALLEGQVSQLDKVLEILHAVKAFDYVRDVAQK
                     EAQMACEALSVFTQSPAKQALLDLAHFSVSRQQ"
BASE COUNT         5031 a         5369 c         4797 g         4593 t
ORIGIN      
        1 atgatggccg acttcatgcg caatcacata ggcttgggca aaatcaccag gtgctttaaa
       61 tcgctcttga agttcctggt aaaaactcag gtcgatatag actttctggt ccgccgggca
      121 ataaaacggc cccatcgcag cttgcccctg cccacaagcg gtttgcgttg cgccgccaaa
      181 aatctccaga ttcggggctg tgtattgctg ccctgcgtct gcaaaagctt tttgccagac
      241 ctgttctgta ctccaaagca cgcgtgaaac aaacttcact tttggatcac tgctatctgc
      301 aggcacggct tgagactcgg cggctggttg cgcctgctga gcaatattga ggaccaggtt
      361 aggatccacg ccaaaataca tggctaccag cgcaatcgca atggtgccta tgcctatgct
      421 gcgtcctccc agcacgctgc gaccacggct gtcttcaaca tgctggcttt cttccaaatc
      481 atccaagcgc acaagaatct cctgaccagt gatattgaaa aatgttgagg gaatgtgatt
      541 actttagcat gcagcactga taaaatcaca ccatgtttac tgggattatt caaaccgtcg
      601 gcaggattgc cgaagtcgtt cctcatggtg aagattgcgc actacgcatt gaagctccgg
      661 cattaggaat ggaagatgtt caactgggtg acagcattgc cgtcaatggc gtttgcctca
      721 ccgtcaccga cttcaactca agcagctttc aggtcatggt ttcaaaagtc acgctggacg
      781 tgaccacggg tttgggccaa gctggcccgg ttaacctcga aaaagccttg cgcctggcag
      841 accggcttgg cggccacctg gtgacaggcc atgtagatgg catcgggacg attaccgcgc
      901 tgcaagaagt gggcgaatgc tggctactca agatacgggc gccgcacgcc atcagcaaat
      961 acatcgccaa aaaaggctca ttgtgtgtca atggcatcag tctgaccgtc aatgagatcg
     1021 atcaggatga agtcagtatc aacctgattc cacacaccat gctgcatact gttatgcagt
     1081 atgccaaggc cggcgacccg gtcaacctcg aaattgattt gattgcacgc tatgtcgagc
     1141 gtatgcaagc atgggagcaa gaataatgtc ggcacaaatc agccctatcg aagcgattgt
     1201 cgccgatatc cgtgcaggca agatggtcat cctggtcgat gacgaacacc gtgaaaacga
     1261 aggtgatttt gtcattgccg ccgaattcgt caccgcagag cacattaact tcatggccaa
     1321 atatggccgc ggcctgatct gcctgaccct gaccgagcaa cgctgccgcc agttggactt
     1381 gccgcctatg gtgcagaaaa atggcacgcg cttaggcacc aatttcaccg tatcgatcga
     1441 agcggccgaa ggtgtcacta ccggtatttc agccgcggac cgtgccacaa ccatacgcgc
     1501 agcgattgcc aaacaagcca atgcgggcag cattgtcagt cccggccata tcttcccatt
     1561 atcggccatg aatggtggcg tactggtacg tgccggacat accgaagcag gttgtgacct
     1621 ggcgcaatta gcaggctgtg agccagccag tgtgatttgc gagattctaa aagatgatgg
     1681 tgaaatggca cgtctgcccg acctgatgca agtcgcggcc gagcacgata tcaaaatcgg
     1741 caccattgcc gacctgatcc actaccgtgc cgcgcacgaa accctgattg agcgcaacgc
     1801 cgaacgcaat attgaaaccg ttttcggccc tatgcgcctg attgcctacc gtgacaatat
     1861 cgctcaagaa acacatctgg tgctcattaa gggcgacccg caaaccagtg atgaagcact
     1921 ggtgcgtgtg catgaaccat tgtccatgat agacctgctg gatagcagcc atcaggcaca
     1981 tagctggaac ctgttgcatg ccatgcgtac catcagcacc gcgccttgcg gggtgattgt
     2041 gctgattaac catgacgaag atggattagg gctcacggac cgcattctgc gtgccgacca
     2101 gaaaatggtt gtcaatcagg agttgcgcaa ttacggtatc ggctcgcaaa tactgatcga
     2161 cttgggtgtc aaaaaaatgc gcctgatggc cgccccgcga aaaatgccga gtatggatgg
     2221 ctttggcctg gaaattaccg gttacctgag tgcagaagaa gccagcccaa tatgagtgaa
     2281 cagttaacgt tgaacaatga tgaggcccgc ctcaacctga ccggtcaaat cctgattgcc
     2341 atgcctgcca tggaagaccc ctattttagt aaaagtgtga tcctggtgtg caatcatgac
     2401 caggacggag ccatgggcat gatactcaat catcccttgc aactgaatgt tggcgacctt
     2461 tttgaacaat tacaaatgaa ctgtgacgcc ccacaccagg aagaacgcca ggtgcatttt
     2521 ggtggaccag tacaggttga gcgaggtttt gtactacata ccccggcaac tgaattcaat
     2581 acgacgttag acctatctga aggactggcc atgacctctt ccaaagatat tctggaagcg
     2641 gctgcccgcg atgaagcgcc acccgagatg tttattgcgc tgggttatac gggctggtca
     2701 gcaggtcaac tggaagagga aattcagtcc aatacctggt taaccctgcc gctggcggat
     2761 acgcaacagt tgcacaaact gattttcaaa ctacccaatg atgacaagct ctcctgggcc
     2821 atgcaacagt tgggtgtgga tttcgccacc ctgtccgagg tggctggtca tgcctgatgc
     2881 ggtcaagcac ggcaaacagc cccttaatca aagccattta ccgattaccc aacgccgcta
     2941 tccagagatc agtggtagcg taattgcgtt tgacttcggt gaaaaacgca taggtgttgc
     3001 tatcggcgat acgctgctca aactggcaca tccgctactc acgatagagg ccgaggaaaa
     3061 tgccgccaag tttgcacaga tagaggctct gctcaacgaa tggcaaccgg cactgttgat
     3121 cgtaggcctg cctatgtccc tggaaggtga ggcgcatgca atgacagcgc tggcacaaaa
     3181 gtttgcccaa cgtctggaag gccggtttaa cctgccagtc atgatggtcg atgagcgcct
     3241 gtcttccgct gaagccgcac aatcattgcg tgaggcaggc atcaagggtc gcgcgcaaaa
     3301 accgttgctg gaccaggtag ccgcgcaaac tatattacaa tcttattttg atgcctatca
     3361 cggacgtgtg taacacagga cgcttatgac attacctgac cccgaacaat tactggatca
     3421 tttataccag caaatcaaac cgcttataca ggaagatacc gccctggttg ggatctatac
     3481 cgggggcgtc tggctgatgg agaggttact caaacggcta caaaccgagc tgggacatga
     3541 catcctgttt ggcaagctgg atgctgccat gtacagggat gactacgcac aacgcggctt
     3601 gaaaaccacc gcgcatccgg gctatatccc attcgacgtg aatggcaagc atatcatcct
     3661 gattgacgat attttttata cgggccgcac gacacgcgcg gtcatgaatg aactgtttga
     3721 ctatggccgc ccggcttcgg taaaactcgc aaccctgatt aaccgtggcg gtgcccaatt
     3781 acctatccgg ccagacatgg taggtgccga cctgcctttg caggcacatc aatcctttga
     3841 cctggccatg aacacgctgg ggcggctaag cctcagtgtt tccgaatctg atccgtctgc
     3901 aaccaaaccg tcagcaaata gtggaaccga cgcccatgta taacccgcaa ctcaacgcgc
     3961 agggacaact gcaacacctg ctcagcatcg aaggcttgcc caaacgtatc ctgaccgata
     4021 ttttggacac agccgaaaac tttgttggtg tcgccgaacg cgaagtcaaa aaagtgccgc
     4081 tactccgtgg caagacggtg tgtaatatct tttttgaaaa tagtacccgc acccgcacga
     4141 cctttgaaat cgctgccaag cgcctgtcag cggatgtact caacctgaat gtaagcacct
     4201 cttcgcagtc caagggcgaa accattctgg atacagtgaa caacctgatt gccatgcacg
     4261 cagacatgtt tgtggtacgt caccagcaaa gtggcgctgc gcattttatt gcgcaacatg
     4321 tgccaccaca tatcagcgtg attaatgccg gagacggccg ccacgcacat cccactcaag
     4381 gcttgctgga tgtattcacc atccgcaaat acaaaccaga aatgcataat ctgcgtgtgg
     4441 cgattgtggg ggatgttttg cactcacgcg tggcacgcag tgaaatccat gccctgacca
     4501 cattaggcgt gccggaaatt cgcgtcattg caccacgcac cctgctgcct actgatgtcg
     4561 aaaagctggg tgtgcaagtg ttccacaata tggagcaagg attaaaagac gtagatgtgg
     4621 tgatgatgtt gcgcttgcaa aacgagcgca tgtccggcgc cctgctgcca agctcacaag
     4681 agttcttcca gacatttggc ctgacccctg agcgcctggc gctcgctaaa ccggatgcca
     4741 tcgtcatgca ccccggcccg atgaaccgtg gcgtagaaat tgattcagcc gtggcagatg
     4801 gcaaacaatc ggtcattctg ccgcaggtca catatggtat cgcagtccgc atggcggtga
     4861 tggcaattct ggggggaggc aacgccgcat gaatatcgta attaaacaag gccgcctgat
     4921 ggatgccagc cagtcactgg accaggtcaa ggacctttat atcagtgcag gcaaaatcgt
     4981 cgccatcggt gaagcgcccg caggctttaa agctgaccaa acgctggatg ccagtggcaa
     5041 atgggtgtta cccggcctgg tcgacttgtg cgcacgtctg cgcgaacctg gtagcgagta
     5101 caaggcgacg ctcttaagcg aactacgcgc cgccatggct ggtggcgtca ccagcctggc
     5161 ctgcccgcca gacaccgacc cggtgctgga tgagcctgga ctggtcgaaa tgctcaaaca
     5221 cagggccaaa tcacaggcac tggcgcatgt gtatccactg ggagctgtca cacgccagct
     5281 agaaggccag ttactcactg aaatgtacgc cttaactgcc gccggctgtg tcggcttttc
     5341 gcaggcagac actgccattg tcgatacgca agtcctatgg cgcgcctttg aatatgccgc
     5401 ttcttttggc tataccctgt ttatgcgtgc cgaagatcct tatctagcca aaaatggtgt
     5461 cgcccaggat ggcgaagtgg cttccagatt aggcttgaaa ggcattcctt ctgcggcaga
     5521 aagcatcgct ttgcaaacca tgctgcgtat tgcgcaagcc actggcgtca gattgcatat
     5581 cgcccgcgtc tctaccgcag aatctgtgga attgatccgc caggccaaac aatccggcat
     5641 gcaagtcacg gcagatgtca gcatccagca tttgcacctg actgatatgg acattggctt
     5701 tttcgacggc atggttaatc tgaaaccacc agtgcgcact caacgcgacc gtgatgccct
     5761 gcgcgcaggt ttggtggatg gtacgctgga tgcagtttgc tctgaccata caccggtaga
     5821 cgatgacgcc aaagctttac catttgctga agcgcatcca ggcgcctccg cactggaact
     5881 gctgttgcca ttgaccttga aatgggcggc ccaagccaaa ctggcacccg tacaagcctt
     5941 acaaaaaatc acgcaaatcc ctgctgatat ccttggaatc aaggcaggca atttgcagct
     6001 aaatagtatc gccgacgtcg tgatctacga tccggagatg gagtggcgtg tggatggtcg
     6061 cgtactcgtc agccagggca agaatacgcc ttttgtgcac catgcactca ccggcaaagt
     6121 cacagccacc ctgacgcaag gaaacttggt cttccagcaa gaaacactaa agctggcgca
     6181 tcattagcgg ttattcaaaa aacaaaacgc ctgggatgca tgaccacatc ccaggcgttt
     6241 ttttggatgg ctaaacaagc gatttacaga atatccaggt tagagatttg gctccgttcc
     6301 tggttctcag gattcatgac tttgctgtta agtttaaact tgaggcgcaa atcattgaca
     6361 gaatccgcat tacgcaaggc atcgtcataa ctgatctggc cagactcaaa caaatcaaac
     6421 aaggcctggt caaaggtctg catgcccagc tcacgggatt tagccatgat ttccttgatc
     6481 gcatgtacat ctcccttgta gatgaggtcg gaaatcagtg gtgaatgcag caacacttct
     6541 accgcggcga ccctaccagt gctatccagc ttgggaatca gacgctggga agcgatacaa
     6601 cgcaggttga gtgacaaatc catcagcaac tgctgcctgc gctcttcagg gaaaaagtta
     6661 ataatcctgt ccaaggcctg gttggtgctg ttggcgtgca aggttgccag gcataagtga
     6721 ccggtctcgg agaaggcaat cgcataatcc atggtttcgc ggtcgcggat ttcaccaatc
     6781 atgatcacgt ccggcgcctg ccgcaacgtg ttcttgagcg cagcatgcca gctctcagta
     6841 tccacaccca cctcacgctg cgtcacaata cagtttctgt ggccgtgaat aaattcgacc
     6901 ggatcctcaa tggtaataat gtgaccataa ctactttcgt tacggtgtcc tagcatggcc
     6961 gccagagagg tcgatttgcc agaccctgtg ccgccgacaa aaatgatcag gccacgcttg
     7021 gccatcacca catccttgag gataggcggc agattaagat catcaaactt gggaatattg
     7081 gtattaatga cacggaacac caagcccacg gcactgcgct ggataaaggc attgacccgg
     7141 aaacggccaa cagaagacat gctgatggca aagttgcact ccagtgtctc cttaaattcc
     7201 tgcgcctgct tgtcgttcat gactgtacgc gcaatctctg ccgtttgctg ggtattcagt
     7261 gtttgcgagg aaactggtgt cagcttgccg tcgattttca tcgcaggcgg gaaacctgcg
     7321 gtaataaaca aatctgacgc cttacgcgtg atcatcagct tcagcaagtc gttgacgaat
     7381 ttctccgtct gggcgtgatc gcccactgtt tgcgtaccat ccatctggtt agccgatgat
     7441 agtgtcacgg tttgcagcct ttgtgcgcgc ttcggcggca ctgatcacat tacgtttcac
     7501 taggtcttgc aggttttgat ccagtgtttg catacccatg ctttggccgg tctggatggc
     7561 agaatacatc tgggcgatct tgttttcgcg gatcaggtta cggatggccg gggtccccat
     7621 catgatttca tgcgcggcaa cacggccctg ctcatccttg gttttacaaa gggtttgtga
     7681 aatcacggca cggagagact cagacagcat agagcgaacc atttcttttt cagccgccgg
     7741 gaacacatcg accacccggt ctatcgtctt ggcagcagag ctagtgtgca aagtaccaaa
     7801 cactaggtga ccggtttcag ccgccgttaa cgccaggcgt atggtttcca ggtcacgcat
     7861 ctcacccacc aggatgacat ccgggtcttc acgtaacgct gaacgcaggg cgttttcaaa
     7921 cgactgcgta tgcggtccca cttcacgctg gttaatcagc gattttttag acgtatgcac
     7981 aaattcgatc gggtcttcca cggtcagcac gtgtgacatc tgtgtttcat tgatgtagtc
     8041 aatcattgct gccagcgtgg tggacttacc ggaaccggtc gggccagtga ccagcacgat
     8101 accgcgtggc gtatctgcaa tatccttgaa cactttgggt gcattgagct cgtccagcgt
     8161 cagtaccttg ctcggaatgg tacggaacac cgcaccggca ccgcgttgct ggttaaaggc
     8221 attgacccgg aaacgggcca ggtcaggaat ctcaaacgaa aaatcgcatt ccagcgtctc
     8281 ttcatacact ttgcgctgac tgtcgttcat gatgtcatac agcatgcggt gtacttcggc
     8341 atgctccata gcaggcaaat taatttttcg cacatcgcca tgcacgcgaa tcatcggtgg
     8401 caggccggag gacaaatgca agtccgacgc tttatttttg actgaaaatg ccaataaatc
     8461 tgaaatatcc acaatcgact ccagtgatta tttatataat gttgctttcg ttttttgtat
     8521 tcttagtgtt taaccacttg ctttcaacca tacgcgctac atcgtcgcca tgactgctat
     8581 ttccgacaac ctcgctttga tccaccaaca gattgctgac gcacgggcac actttaatgc
     8641 gccgcaaaac gtcacgctgt gtgcagtcag caaggcgcaa cctgctgagg ctatccgcgt
     8701 cgcttacgag gctgggcaaa ccgtttttgg cgaaaactac ctgcaagaag ccttggctaa
     8761 acaggaaaag ctacaggact gtgcaatagc atggcacttt atcggcccga ttcaaagcaa
     8821 taaaacacag ccaattgccc gtcaattcgc ctgggtacac tcggttgacc gccttaaaat
     8881 tgcacaacgc ctgtcagatg cacgcccgct tgagcttgcg ccactgaata tttgcctaca
     8941 agtcaatatc agcgaagagg caagcaaaag tggtgccagt ggtcaggagc tattggaact
     9001 cgccttaaat atcaaacagt tgccgcgctt gcaactacgt ggtttgatgg caattccagc
     9061 accttgcagc gatattgagc agcagcgtga ccaatttcgg caggtgcgcg cattatttga
     9121 gcaattgaac aatcatggcc tgcaactgga taccttatct atcggtatga gtggtgattt
     9181 tgcagcggcc attgccgaag gcgccacatt ggtacgcata ggcaccgcta tttttggtgc
     9241 gcgtacaaaa cctttttcta aagactcagt gaaccaagga acctgacccc catgcaatca
     9301 ttctcagcgg cacgcattgc ctttatcggc ggcggcaata tggcacaggc cctcatgata
     9361 ggcctgcaac aacgcgcatt cgccatgcag aacatcaccg tgattgaccc ggacacagcc
     9421 aagcacgaac atttgcaagc aacgctaggt gttaatacca gcgccagcct gactacagga
     9481 tcacttgctg ttgatgtagt cgtactggcc gttaaaccgc aacaactgca cagtgtggca
     9541 caagccctgg cgcctttatt gcaatctcaa ttagtcgttt cggtggcagc ggggatacgc
     9601 acggcagatt taagccgctg gttaaatggt tatacaacca tcatccgcac catgccaaat
     9661 acacctgccc agattcagtc cggcatcact ggcgcgtatg ccttgccagc tgtcacgcaa
     9721 gcgcagcggg agatcgcaga tgccatgcta caagctgccg gagaagtggt ttggctgaca
     9781 gatgaagacc aactggatgc ggtcacagcc atttcaggta gcggaccagc gtatgtattc
     9841 ctcatgatag aggcattggc cgccgcgggc ctcagtttag gtctgagtga agcccaatcg
     9901 ctgcagctga gcctggccac ctttaaaggt gccagcttac tggcagctgg cagcactaca
     9961 cctgtcgcca ccctgcgtga acaagtcacc tccaaaggcg gcacgaccga acaggggctg
    10021 ctcagtatgc gccagcacga tattcatggc atgatgcagc atgccgcaga acaagccgca
    10081 cgccgcgcca aagaactggg tgatcaactg ggagcacaag catgatgttg caaaacatga
    10141 caactttttt actgaatgcc gtgtttggca tcctgacgtt tttactggta ttgcgttttc
    10201 tgatgcaatg gacacgcact tcattccaga atccactggg gcagatgacc atggcactca
    10261 ccgattttat ggtgaagcct gcccgcaaga tcatccggcc agccagacaa tgggacatgt
    10321 ctacgttgtt gctggcatta ctgatgcaga ttgtgttgtt cgcactctta acattgttgg
    10381 ctggcgcacc agcttcgcca ttcttctgga tatggcaagc catatttggc gtgctggggc
    10441 aaatcgtgga tgtgtttttc tatgcgatat tactgatggc cattttaagc tgggtgaatc
    10501 cttacagctc gatttacggt gtcttgaacc agctgtcgtc ccctatcctg gatccattgc
    10561 gccgcgtgct gcctcagatt cagggctttg acttttcagc cctggtggcg ctgatattgc
    10621 tgcagatgat tagccacatt gtcctgcccg gcctggctgc cggcatcttg taaagtatct
    10681 cgagagcgtc aactcgctct cgagattaca tccacacgat atcgaattct tcccgcccat
    10741 actgggtgtc cgggtgcagc gagagtggtt ttttaataaa gtctgacaaa ttcgccaggc
    10801 tttgtgactc ttcatcaccc agcatgttaa tcacttctgg cgatgccagt atcttgaatt
    10861 ccttggcgtt tctgaactgc ttggactcgc gtaacaactc acgcatgatc tcgtaacaaa
    10921 tggtttgcgc cgttttgagc tcgccacggc cctgacagac ctggcaagac tcacacagca
    10981 gatgcgccag gctttcgcgc gtacgtttac gcgtcaactc caccaggccc aatgccgaaa
    11041 aaccattcac attgatgcgg gctctatcca gcatcacagc tttgcgcaac tcttccagca
    11101 cagcttcgcg ttgcgcatcc tcatccatat caataaagtc gatgatgata atgccaccca
    11161 ggttacgcaa tcgcagctgg cgagcaattg cctgcgccgc ctccaggttg gttttaaaaa
    11221 tcgtatcgga caggttcttg ccactgacaa agctaccggt attcacatct accgtggtca
    11281 gtgcctcggt ctggtcaaaa atcaggtagc cgccagactt cagctctact ttgcgcgcaa
    11341 gagccttttc gatctcgttt tcaatatcgt aaaactcaaa cagcgaacgc tcgcccttgt
    11401 agtgcgtcaa gggttttacc gcatgcgaca catataactc ggcaaactct ttcaggcgct
    11461 caaacatctc gcgggagtcc acgcggatac tggtagtttc agtacacacg acatcgcgca
    11521 gaatgcggcg tggcaggctc aggtcataaa aaatgagtga tcgttcgcca accttggtgc
    11581 tttctgcctg aatcttgctc cagactttgc gcaaatattc aatatcggcc agcaactctt
    11641 catccgtcgc attttctgac atggtacgga taatgtagcc tccttcacgc tcctcgggta
    11701 acaaacccag cagacgattg cgtaagaatt cccgctcctc ttcgatatca atgcgctggg
    11761 aaacaccgat gtgttgctga tagggtaaat agacgagaaa gcgtccggca atactgattt
    11821 ctgtcgtcaa ccgcgcacct ttggtaccga tggcttcctt gatcacctgc acgatgattg
    11881 attgcccttc gtgcaatacc tcctgaatcg ggcgctcctt cttgatatcg ccttcctcag
    11941 catggcattc catgatatcg gccgcatgca gaaaggcagc tcgcggcaat ccgatctcaa
    12001 caaaagcaga ttgcatcccc ggtaatacac gcaccacagt cccacggtag atattaccga
    12061 caatccccag tgaagaagcc cgctcaatat gcagctcttg tacgacacct tgttccagaa
    12121 tcgctacccg cgtctcctgc ggcgtgacat taatcaatac ttcttgcgcc aatgacatat
    12181 tgctttaccc gatcccgatc attatctttt gtgcaaagtt taccatgcgt gcagcaactg
    12241 cgccgtttca tgcaaaggca aacccatcac gccgctataa ctaccatcga tacgtgcaat
    12301 ccaggccccc gcacgcccct gtatgccata cgcgcccgct ttatccatag gttcaccaga
    12361 agccacatac gcttccaaga ctgtcaccgg gatctgcatc atttctactt tggtactatt
    12421 gagcaaatac cgtaactgcc cagcttgata cactgcgact gcggtatgca ccatatgaat
    12481 actgccactt aagcgcgaaa gcatcgccat cgcctcacgg gcatcagccg gcttgcctaa
    12541 aatatcacgc cccagagcga cagtagtatc tgctgctaga attgccaagc cacgctctcc
    12601 atgtgtcgca gcgactgcga gggctttttc ctgtgccagc cgcaacacat agtgtgcggg
    12661 gtcctcaccg gggacactgg attcgtctat atcagcaggc gcaatctcac actgcacacc
    12721 taactgagct aacaattcga cccggcgcgg gctgcgcgaa gccaatataa tattgcgttg
    12781 ttgcatattc tttatcgcga tttctatttc aattaaggtc gacaaggtaa cacaaacaca
    12841 gggagttttg cgctaaagaa agtccaagtt gcgacccaac ttggactaac aggcgcttgc
    12901 cccattgtgt acaataactg ctcgctttct tttctcatcc atactatccg catgaactca
    12961 cttgaacact tgttgcagcg tgccgaacaa ctgatcacgc gcctcgaaac cctgctgcct
    13021 gccccacctg ccgaaaccaa ctggcaagcg gttgcctggc gctgggtcaa acatggcgca
    13081 caaggccatt tacaagtggt agcgcacccg catgcagtgt tgctggaaga catacaattt
    13141 attgaccggc aaaaaaccga ggtggtacgc aacacccggc aattcctgca aggcttgcca
    13201 gccaaccatg tgttacttac gggggctcgc ggcacaggta agtcatcctt ggtcaaagcc
    13261 ttgctcaaca gctatgccga acaaggctta cgcctgatag aggtggaaaa acaggacctg
    13321 aatgacctgc cacaaattgc acaactgatt cgcctacggc ctgaaaagtt tattatcttt
    13381 tgtgacgacc tcacctttga agccaacgat attggctaca aagcgctgaa agtcatcctg
    13441 gatggttcga ttgaacacgc atcacccaat ctgctggtct atgccacctc caaccgcaaa
    13501 aacctgatgc cggagtatat ggccgacaac cagccgatta tagacaaagg tgaaatccgc
    13561 cagaacgaca ccatagacga aaaaacatcg ctggctgagc gttttggctt atggctctca
    13621 ttctacagtt ttgatcagga tgaatacctg agcattgccg aacattggct gaaaaccttt
    13681 ggtatcgcca tggatgaagc tgcccatcac gccgccttgc agttttacca tacccgtggc
    13741 gcccgcagcg gccgtgtcgc ttaccagttt gccaaagact acgccggcca actgcaactg
    13801 aagtcctgac catcatgaca aaactcgttc aagtcgcggt tgcgatactc atgcaaccca
    13861 atggtgaata cctactcgca aaccgcccta gcggcaaagg ctgggccggc tggtgggagt
    13921 ttcctggcgg caagattgaa gcctgcgaaa cgccagagca tgctttgatc cgtgagtcgc
    13981 aggaagaact gggtattacc ccaacgcaaa tacagccatg gatcaaacgc cgctacgatt
    14041 acccggccac acatgatgcc gaagccaaaa ctgtattgct gcatttcttt tttgtctacg
    14101 cgtggcaagg cgaattgcaa gcacgcgaag gccaggagtt tgcctggcaa catccacaaa
    14161 agctcaatgt caccccggtg ctgcccgcca atgcgcccat tatgcaagcc ctgagcatgc
    14221 cgccggtgta tgccatcagc aatgtgtcgg aaatggtcgt acctgcattc ttgcaagcac
    14281 tcaagcagca acttgagcaa ggcctgcaac tgctgcaaat ccgtgagcca cagcttaatg
    14341 acacagcaat cgccaaatta agcgaacagg tattacagct atgcgcccat tatgactgcc
    14401 gctgcctgct caacggtagt cccgagcaag ccctacaact tggctaccac ggcgtacatt
    14461 taaatagcca gcgtctgatg gcactcacac aaaagccgga taacttgtta gtgggtgcct
    14521 cttgtcacga tgtagagcaa ctgcaacaag cacaagccct gcaactagac ttcgccctgc
    14581 tctcacctgt gctaccaaca aaaagccacc ctgaagcgcc tggattaggc tgggaaaaat
    14641 ttgctgaaat gctgagtggc ctggaagtcc cggtgtatgc gctgggaggc atgcacctgg
    14701 aacacctatc acaggctcaa tcctgcggtg cgcgtgggat cgcaatgcag cgagctatat
    14761 ggtcaacaac gtgacaatca gagaaactcc ccaaggactc gaatacatat gatcactaag
    14821 caactgacgc actaaatcat gcaaccacct caaccaaaca accctttaca cggcgtgaca
    14881 cttgagtaca tggtcgaaca actggtcgag cattacggct gggatcaact tgcgcaaaag
    14941 gtgaatatta attgctttaa gaatgaccca tctgtgaaat ccagcttgaa gtttttgcgg
    15001 cggacgtcgt gggcacgggc tgaggtagag cgcttatatc tcgagacttt tgcccaaaat
    15061 cacggatgat gttgattgga gcttaatccc cacagcaatc gtttcaggtc agggtgctga
    15121 tttgaccggc atggtgacaa actggtcggt gatcttgcct tgtttgtctt taaaacagag
    15181 cctaaatttg acttgttcac cagctttaaa cggtgttgcc aattcaaata gcatcaagtg
    15241 taaaccacct ggtgcaagct tcactggttt gccagcgggc accggtaatt cttccatact
    15301 acgcattttc atgatgccgt tttgcatggt catgctgtgc atttctacgg tgcctgcacg
    15361 ctcggtttcg gcatagacaa gtgtgacatc ttctgtgctg gataatgtga cataggctgc
    15421 acctactgat tgcccgggat tggaggcacg cacccaggca tcagtgagct ttacctctgc
    15481 atgtgcaatc cccaaaaagc cgagcaggca aaggctggca acaagacgaa gaaagaaagc
    15541 tgttttatgc atggtcaaac tcctggcagc cagctattct ggctcgtata aatcaggtgc
    15601 cgtgctatct ggaatacgga atttctctgt agcccaatca cccaaatcaa ccagtttaca
    15661 gcgctcactg caaaatggcc ggaaagggtt ggcaggagaa tactcggtgg tttcaccgca
    15721 agacgggcag gccactttac gtgctttgac tgcaggctgc gtcaattgtg gccagctccg
    15781 gtaaagttac agataatcat cttgaacgag atatcctggt cacagccttt agggcgctgc
    15841 acaaaatcca gtttctggaa gcggatatta atggcgtatt tgttggcact gacttcaggg
    15901 aagcattcgt cgtggtcaat ttcaatgcgt aataattgcg caggtttatg ccctgacaat
    15961 tgctgctgat aggcgccatg atgcgctacc aatgacaaca cttggccact gccacgcaat
    16021 aattgcagta tcaggcggat cgcttcgtac atcggcatga gtttagtaag ccaatgatca
    16081 aagtcttttt tacggcgctc aacaccgagg taaagccaat gccgataaga aggcaagtca
    16141 aactcgcata cgccaccagg aatactcgtg cgttgcttaa cgctcattaa ccagtcattc
    16201 tctcgtaatg attgccccag tttctgatgg tctgagcgca aggcagttgc gcagcgatcc
    16261 aggccggata acgtactcgc caaggcttgc tgatcgatat taggattatt ttgcaggaat
    16321 gataactgga ctttgtgcct gtccagttct tgtaacaggt cgacctttaa atccccgcga
    16381 tcaatcaggt ccaggacttg cagcatggaa atcagggcca catgatgact gatctcatgt
    16441 ccgatctcaa tgtggtgcag catcttgatg aacagatctt ccaggcggag ataggttctg
    16501 atgcgttcgt taaatggaaa ctcgtagctc gacacggcag ccttttctgt cagctgatac
    16561 ttgaaaagta tgcaataaaa gcctgattat gaaacccttt tcattcactg gcaagtttta
    16621 gagaaatttt tatgaaactc tagcactttt tcctgcaaat tatctaatgt ttgctcgttc
    16681 acaatcactg aatccgcaat ggataatcgc tcttcacggc tgctttgcgc agcaatcatg
    16741 gcctgggcca tggtttcaga taactggcta cgctgcacaa tacgcgccag ttgcgtctgt
    16801 tcgtcacaat caacgactaa tacatgatct atcatctgcc aatcctctcg actctccacc
    16861 agcagcggaa tcgcaagaac gacataagcc tgcgcagagt accctgcaat ctgcctgaca
    16921 gcttcagccc tgattgccgg gtgcataatc tgattcagct gctctaatgc atcgggctca
    16981 gcaaacacca gttcacgcaa tttggcacgg ttcaaacggc catcaatctc aaacatttca
    17041 tcaccaaaag tggtgcgcac tgcctgcatg gcaggactgc cgggcatgct catttcatgc
    17101 gcaatctggt ccagatctac cactggcaca cctaaagccg aaaagtattt gcacacctcg
    17161 gttttaccac taccgatacc accagtgaca gcgactactt tgctcatcaa aactccacta
    17221 cgctatagct actatggcac caggtaataa gaagccaatg cctggccata aaacaaagca
    17281 gcaatgccac ctaatgccag aaatgggcca aatggaatag cggtttgcct ggttttgcct
    17341 ttaagcagaa tcaagcctat gccaatcacg ctacctacga cagagctcag caaaatcacg
    17401 gccggcaaca actgccagcc aaaccatgcg ccgatcgccg ccagcagttt gaaatcacca
    17461 tatcccattc cctctttacc ggtgaccagt ttaaacagcc agtatacaga ccataacacc
    17521 agataacccg ccatcgcgcc gatcacggcc gaagtaaggt cggtaaatcc gtacttgata
    17581 ttcagcaaca gtcccaacca caacaagggc agcgtgatgt catcaggcaa taactgggta
    17641 tcaaagtcaa taaaggtcaa ggccacgagt gcaaacacaa acaccagggc aaataaggtg
    17701 agcatgccgt agccatattg cactccaaca gccagtgaca ataatccagt cagcaactcc
    17761 accaacggat aacgcgcgga aatcggggtt ttacagcctt tgcaacggcc acgtaaaaac
    17821 aggtagctga tgacaggaat attttccagg gcggtgatct ggtgtccgca atgcggacag
    17881 gctgagcgcg gcacaaccag attgtatttg ggttgttcgg gtgctgtttg cccttgcaaa
    17941 tccagacagc tggcatgcca ctcacgctcc atcatttttg gcaggcggtg tatcacaaca
    18001 ttgaggaaac tgccgaccag caaaccgagc actaaacaaa ccgtttgcaa gaaatgcggc
    18061 tgcgtgcgca acaaactcaa aacaggctca aggatttctg acatgatacc gataacaaag
    18121 ttaaggttgc tgcatgatct catcgttaat gcaagtaagc aaggctcaaa gatggcaaaa
    18181 cctgtttaaa agctgctttt tcggtcttga aaaccgcttg cataccgcca tttatgtgta
    18241 agatgggaat agcactccag ccgagtgact tcgcatccat tatcaagtga aggaagttgc
    18301 ctatgtctga ccaaatcaaa tttacgccac cgactgaagt cgaattgaac caggtcgaac
    18361 atcctgcgtg ggatgagcct gtgatttgtc gtgtagaagt cgatttacct ggatggctaa
    18421 ccaaactgac cgggcaagca cattgggagg tttacagcga ggaagaagaa gagaattgcg
    18481 tcagctttgc catgcgctct tcccatgcac gtcacttgca taaagcaacc acgcctatta
    18541 gggacgacat gcaagctgag gttacgctct accacaacgg ttacgcagtg gtagatgtgg
    18601 atggcaaatc gctatttgac ggtgcattaa ccaacgctac caacgattgc gcgcgcttga
    18661 gctactacca catgagtggt gagcctatcg cgttgaatta accctctgaa cttcagcctg
    18721 ccaggaaacg gcaggctgct tgcttattgc tggcgactta ccgaaaaatg agccagatcc
    18781 agtaaagcct gtttcgcagg tgactgtgta aacaccgaca aagcctcaca ggccatttgc
    18841 gcttcttttt gcgccacatc gcgcacataa tcaaacgctt ttacggcatg taatatctcc
    18901 agcactttat caagttgcga gacctgccct tcaagcaaag cctgctttac caaatcagct
    18961 tgcgctgcag gcgcttgctg tatcgcatag agcatgggca aggtcacctt accttctgcc
    19021 aggtcgttgc ccagtgtttt gccgattttt tcgctttcac ccgtcaagtc cagcacatca
    19081 tcaatcagct ggaacgccgt gcccaaatgc atgccataag tcgccaaacc ttgttcctgt
    19141 tcagcactca cctggcactg tacagcaccc agcctggtcg cggcttcaaa taacttggca
    19201 gttttaaagt gaatgacttg caggtaatcc tgctcgctga cgccaatatt acgcacattc
    19261 agcaattgca gtacttcacc ttctgagata acgttcgtgg catcagccag aatttccatc
    19321 acgcgcatat tctgcagttt caccatcatc tggaacgagc gagaatacaa gaaatcccct
    19381 accagtacgc tggccggatt gccatagaca tgatttgcag tggatttccc acgccgtaac
    19441 tccgaggcat ccacgacatc atcatgcaac aaggtcgcgg tgtggataaa ttcaataatg
    19501 gcagccagcg aatggtgttc ggaagtgacg gcaccggcca ggtcaccaga catcaacacc
    19561 agtgccggac gcaagcgctt gccaccacta tgaatgatgt attccgaaat ttgattaatc
    19621 agcggaactt gggatgacaa agactggcga ataacggcat ccacctgctg catatcgcct
    19681 gcaatccgcg ccaaacaagc atttagagac actgaaacac caatgaaaaa acaaagttaa
    19741 agcataccat atctgcgata cctctcgcta tgctcaacgc actagaatag
//
LOCUS       DATMSK010000255         5185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_303117,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000255 DATMSK010000000
VERSION     DATMSK010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303117"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1242
                     /locus_tag="VNZ84_08540"
     CDS             34..1242
                     /locus_tag="VNZ84_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HWU67045.1"
                     /translation="MTNQQDSMHACPACGLLCDDISGDAITRQQFSCGKAAKFYARVT
                     SGAKPQVAGRSVPIDEAVKAAANLLKQAKAPLIAGSSTDVHGARALVNLSHRTGAAMT
                     HINAGSTLRNMKVLQHRGWQTTTLTEVRNRSDVILMIGTDVVSHNTRFFERVVWVNDA
                     MFTEPAARKIIYLGGDQLNTKPGVAPDGRAPEVISCASEFLPEVVATLRALVMDKPVT
                     AETVAGVAVSSLKAVAETLKAAKYATLVWVSKDLHYDHAELTIENITETVVALNQKSR
                     AMGLSMGGSDGDTSVNYAHTWLNGVIIDAPEWESHDAVVWVNSYSPDAMPPAGSGPVI
                     VLGAPDSTFDAAPDVFIPVATPGLDCNGQQFRVDGSVTLPLTAAKPSDLPTLSQVVAL
                     IEAQLQGDLA"
     gene            1239..2903
                     /locus_tag="VNZ84_08545"
     CDS             1239..2903
                     /locus_tag="VNZ84_08545"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HWU67046.1"
                     /translation="MITLLKNAKVIDPAHNKDGVVQDIYIRDGRIISKPADSEKIGQV
                     HDLNGKIVMAGAIDMHSHIGGGKVNIARMLLPEYQAMRQLEEPEAHICGANCSHHATP
                     NTTDTGMRYIEMGYTAAFEPAILAVNARQAHMEMGDTPMIDKGGYAMLGNDDYLLRLL
                     SHNADQKTINDYVAWTLHATQTIGIKVVNPGGISAFKFNERRQDLDQLHSFYNITPRR
                     ILQALSRAVNDLGIAKPLHVHCNNLGVAGNFQTTLDTMGASDGIPMHLTHIQFHSYGT
                     EGDKKFSSAAANIAEAINTNKHITADVGQILFGQTVTASGDNMMQHLNAKVANPKKSV
                     IMDIECDAGCGVVPFKYRDQNYVNALQWAIGLEVFLSVDDPWRVFLTTDHPNGAPFTS
                     YPHLIRLLMDKTFRNDAFDKLNLDAQAMSNLKSLDREYSLYEIATMTRSAPAKLIGLQ
                     DRGHLGIGAAADITVYTDQADKEAMFAKPDLVFKDGELVVKEGKVVKVVWGATHTAKP
                     AFDTSVEKDIKQYFDRYHTMQMQNFKISNDHIVEDGRNHVINHAQG"
     gene            2906..3832
                     /gene="fhcD"
                     /locus_tag="VNZ84_08550"
     CDS             2906..3832
                     /gene="fhcD"
                     /locus_tag="VNZ84_08550"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HWU67047.1"
                     /translation="MVINGVEIDDTFAEAFNMRGTRILITAQNLRWAYNAANAMTGFA
                     TSVIGCGVEAGIERELSEDETPDGRPGLSVLMFAMGSKVLMQQLETRMGQCILTCPTA
                     AAFAGIESDDMISLGKHLRFFGDGYQVSKQIPDANGKLKRYWRIPVMDGEFLTEETTG
                     MVRAIGGGNFLVLGASQAQVLTACEAAIDAMRKLPNVIMPFPGGVVRSGSKVGSKYPK
                     MFASTNYAFCPTLKGVVKSELDPRVESVMEIVVNGLTFEDIAISMKAGIEAACSLGKD
                     NGILRISAGNYGGKLGQHHFKLRPILSGEVTA"
     gene            3829..4500
                     /locus_tag="VNZ84_08555"
     CDS             3829..4500
                     /locus_tag="VNZ84_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HWU67048.1"
                     /translation="MSHIVLTAKPISRNVDCRTLLPVALQGKTVAEIAAMKLAANLSV
                     ADVFEVNVDTASEAAVVTFKNTTAAHQYIGFGMTAGQINVAGDAGDFLGAQLQQGVLV
                     CKGNAGARAGDRMRRGMLLIEGNAGDYCGSDMMAGTLGVLGSTGAHLGYGMKRGTLLL
                     AQTPAPQATWVDCGFHKLPFLNILYKSFKQLDSRFAQISSQRVQRWMGDMGGLGKAEI
                     LVIQS"
     gene            4609..>5185
                     /locus_tag="VNZ84_08560"
     CDS             4609..>5185
                     /locus_tag="VNZ84_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HWU67049.1"
                     /translation="MSQYLTAALYKFVTLDDYKDLQQPILEACQKHQIKGTLLLAHEG
                     INGTIAGQPEDIRAVLDYLRQDPRLADLEHKESWADSHPFYRMKVKLKKEIVTMGVPE
                     VDPNLVVGTYVKPEDWNAIISDPDVIVLDTRNDYEVHIGTFKGAVDPKTTTFREFPKY
                     VADNLDKTKHKKVAMFCTGGIRCEKASSYMKQ"
BASE COUNT         1299 a         1255 c         1428 g         1203 t
ORIGIN      
        1 tacggaagat caaaacgaga gacctaacca gccatgacaa atcaacaaga cagcatgcat
       61 gcttgcccag cctgtggctt gctatgcgac gatatttcgg gggatgcgat taccagacag
      121 cagttttcgt gtggtaaggc cgcaaaattt tatgcacgag tgacctccgg tgctaaacca
      181 caagtcgccg ggcgaagtgt gccaattgac gaggcggtca aagcggcggc aaacctgctt
      241 aaacaagcca aagcaccatt gatagctggc agcagcaccg atgtgcatgg tgcacgtgca
      301 ctggttaacc tgtcacaccg cacgggtgct gccatgacac atatcaatgc cggtagcacc
      361 ttgcgtaaca tgaaagtgtt gcagcaccgc ggctggcaga ccaccacatt gacagaagta
      421 cgcaaccgtt cagatgtcat tctgatgata ggcacggatg tcgtgagcca taacacacgt
      481 ttttttgagc gagtcgtgtg ggtgaatgac gccatgttta cagagcctgc agcacgcaaa
      541 attatttatc tgggtggcga tcaactgaac accaagcctg gtgttgcgcc ggatgggcgt
      601 gcacctgagg tcattagctg cgcttctgag tttctgccag aagtcgtcgc cactttacgc
      661 gcacttgtga tggataagcc cgtgacggct gaaaccgtag caggtgtggc cgtgagcagt
      721 ttgaaagcag tggctgagac gttgaaagca gccaaatatg cgaccttggt gtgggtttcc
      781 aaagacttgc attatgacca cgctgagctc accatcgaaa acatcaccga aaccgtggtg
      841 gcgttaaacc agaaaagccg tgccatgggt ttatccatgg gtggcagtga tggtgatacg
      901 agtgtgaatt atgcgcatac ctggctcaac ggtgtcatta ttgatgcgcc agaatgggaa
      961 agccatgatg cggtggtgtg ggtgaatagt tacagcccag atgccatgcc gcctgcgggg
     1021 tcgggtcctg tgattgtgct aggtgcacct gacagcacat ttgacgcagc tcctgacgta
     1081 tttattcccg tggcgacgcc cggtttggat tgtaatggcc agcagttccg tgttgacggt
     1141 tcagtcacct tgccgctgac cgcggccaaa ccttccgact tgccaacctt atcgcaagtg
     1201 gtggccctga tagaagccca attacaagga gatttggcat gattacgctg ctcaaaaatg
     1261 ccaaagtgat agaccctgcg cacaataaag acggggtggt gcaagacatt tatatccgcg
     1321 atggccgcat tatctccaaa ccggcggata gcgaaaagat cggccaggta catgatctga
     1381 atggcaaaat tgtgatggcc ggtgcgattg atatgcatag ccatattggc ggtggcaagg
     1441 tcaatattgc ccgaatgttg ttgcctgagt accaggctat gcgccaattg gaagaacctg
     1501 aagcgcatat ttgcggtgcc aattgcagcc accatgccac gccaaacacg accgatactg
     1561 gtatgcgcta tatcgagatg ggctataccg cggcgtttga gcccgcaatc ctggccgtga
     1621 atgcgcgtca ggcgcatatg gaaatgggcg atacgcccat gatcgataaa ggcggttatg
     1681 ccatgctggg caacgatgat tacctgttgc gcctgctgag ccataatgcc gaccagaaaa
     1741 ccattaacga ttatgtagcg tggacattgc acgcgacaca aaccataggc attaaagtgg
     1801 ttaacccagg tggcatttcc gcgtttaaat tcaacgagcg ccgccaggat ctggaccaac
     1861 tccacagttt ttacaacatc acgcctcgcc gtattttgca ggcgctcagt cgtgccgtga
     1921 atgacctggg catcgccaag ccactacatg tgcactgtaa caacctgggc gtagcgggta
     1981 atttccagac cacgttagac acgatgggcg cgagtgatgg tatccccatg cacctgacgc
     2041 atatccagtt ccacagttac ggaacagaag gagataagaa gttttcttca gcggcagcta
     2101 atattgccga agcgatcaat accaacaaac atatcacggc cgatgtcggc cagattctgt
     2161 ttggtcaaac cgtgacggct tctggtgaca atatgatgca gcacctgaat gccaaggttg
     2221 ccaacccgaa aaagtcagtc atcatggaca tcgagtgtga tgcgggctgc ggtgtggtgc
     2281 ctttcaaata ccgtgaccaa aactatgtca acgccttgca atgggcgatt ggcctagagg
     2341 tgttcctgag tgtcgatgac ccttggcggg tatttctgac gacagaccac ccgaacggcg
     2401 cgccgtttac cagctatccg catttgatcc gcttgctgat ggataaaacc ttccgcaatg
     2461 atgccttcga taagctcaat ctggacgcgc aggccatgag taacctgaag tcgctggacc
     2521 gcgaatacag cctgtatgaa atcgccacca tgacgcgttc agcaccagcc aagttgattg
     2581 gcctgcaaga ccgtggccat ttaggcatag gtgcggctgc cgatattacg gtgtataccg
     2641 accaggctga caaagaagcc atgtttgcca agcctgacct ggtgttcaag gatggcgaac
     2701 tggtcgtaaa agaaggcaag gtggtaaaag tggtgtgggg tgcaacgcat accgccaagc
     2761 ctgcctttga taccagtgtg gaaaaagaca ttaagcaata ttttgaccgc taccacacca
     2821 tgcaaatgca aaacttcaaa atttcgaatg accatattgt ggaagatggc cgcaaccatg
     2881 tcattaacca tgcgcagggg taactatggt aattaacgga gtagaaatag acgatacctt
     2941 tgccgaagca tttaatatgc gcggcacacg catcctgatt accgcgcaaa atctgcgctg
     3001 ggcctataac gctgccaatg ccatgacagg ctttgccacc agtgtgattg gttgcggggt
     3061 agaggcgggt atagagcgcg aactgagtga agatgaaacc ccggatggcc gcccaggcct
     3121 gagcgtgctg atgtttgcca tgggtagcaa agtgctgatg cagcagctgg aaacccgtat
     3181 gggccaatgt attttgacct gcccgacggc tgcagccttt gctggtattg agtctgacga
     3241 catgattagc ctgggtaagc atttacgctt ttttggtgat ggttaccagg tatccaaaca
     3301 aatcccggat gcgaatggca agctcaaacg ttactggcgt atcccagtca tggatggcga
     3361 atttttgacc gaagaaacca cggggatggt gcgcgccatt ggtggcggca atttcctggt
     3421 attaggggcc agtcaggcac aagtgctgac cgcgtgtgaa gccgcgattg atgccatgcg
     3481 caaactgcct aatgtgatta tgcctttccc tggtggcgtg gtgcgctctg gttccaaggt
     3541 cggtagcaaa tatcccaaaa tgtttgccag caccaattac gcgttctgtc cgacactgaa
     3601 gggcgtcgtc aagagcgagc tggatccgcg cgtggaaagc gtgatggaaa ttgtggtcaa
     3661 cggtttaacc tttgaggata ttgcgatttc gatgaaagcg ggcattgaag cggcttgcag
     3721 tttaggcaaa gacaatggca ttttgcggat ttctgctggt aactacggcg gcaagctggg
     3781 ccagcatcat tttaaattgc gcccaatttt aagcggggag gtgaccgcat gagccatatc
     3841 gtattaacag ccaagccaat ctcgcgcaat gtggattgcc gcactttatt gccagtcgcc
     3901 ttgcaaggca aaacggtggc tgaaattgcc gccatgaagc tcgcagcgaa cttgagtgtg
     3961 gccgatgtat ttgaagtcaa tgtagatact gcgtccgaag cggcggtggt gacatttaaa
     4021 aacacgacgg cagcccatca atatattggt tttggcatga cagccgggca aatcaatgtg
     4081 gcaggcgatg ctggtgattt tttaggcgca caattgcaac agggcgtgct ggtctgcaaa
     4141 ggcaatgctg gcgcacgtgc gggtgaccgt atgcgtagag gcatgttgct gattgaaggc
     4201 aatgccggtg attattgtgg ctcggatatg atggccggta cgcttggcgt gcttggcagc
     4261 accggtgccc atcttggtta tggcatgaaa cgggggaccc tgttacttgc gcagacgcct
     4321 gcgccacaag cgacttgggt agattgtggt tttcataagc tgcctttcct caacattctt
     4381 tataaatcct tcaagcagct ggatagccgc tttgcacaga ttagcagcca gcgtgtacag
     4441 cgctggatgg gtgatatggg tggcttgggt aaggctgaaa tcctggtcat ccagtcttag
     4501 ctgtcagctt catccattcg agatcaacgg gcctggtgcc cgtttttctt tttgcatcag
     4561 tgggttagag tggtaaaatc gcgcttttga aataaaagct gtattcacat gagtcaatac
     4621 ctcaccgctg ccctctacaa attcgtcacg ctggatgact ataaagacct gcaacaacca
     4681 attttagaag cgtgccagaa acaccagatc aaaggcacgt tgttgctggc gcatgagggt
     4741 atcaatggca ccattgccgg tcagcctgaa gatattcggg ctgtgcttga ctatttgcgc
     4801 caggatcctc gcctggctga tctcgaacac aaggagtcct gggcagactc tcatccgttt
     4861 tatcgtatga aggtcaagct caaaaaagag atcgtgacca tgggcgtacc tgaggtagac
     4921 ccaaacctgg tggtaggcac ctatgtgaag ccggaagact ggaacgccat tatttctgac
     4981 ccggatgtga ttgtgctgga tacccgcaat gattatgaag tgcatattgg cacctttaag
     5041 ggcgcggtcg atcctaaaac caccactttc cgcgagtttc ccaaatatgt ggctgacaac
     5101 ctggataaaa ccaaacacaa aaaggtcgcc atgttctgta cgggcggcat ccgctgtgaa
     5161 aaggcttcct cctatatgaa gcaac
//
LOCUS       DATMSK010000256         6173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_231745,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000256 DATMSK010000000
VERSION     DATMSK010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231745"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VNZ84_08565"
     CDS             complement(<1..643)
                     /locus_tag="VNZ84_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HWU67050.1"
                     /translation="MAYPIEKKLVISVASSALFDLTESHEVYKTQGVDEYRRHQEVNI
                     DVPFPKGVAFPFIRRFLAINKAFPAELPVEVVLLSRNSPETGLRVFRSIRHYGLDITR
                     ASFMTGRSPHEYIPAFNTSLFLSANEEDVQSAIDLDYPAGVVLPSKVYDDEADTELRI
                     AFDFDGVIADDEAEAVFKMNNDLGEFEAHEVEKQSIPHQAGPLADLFKKLSFMQ"
     gene            complement(679..2565)
                     /gene="mutL"
                     /locus_tag="VNZ84_08570"
     CDS             complement(679..2565)
                     /gene="mutL"
                     /locus_tag="VNZ84_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWU67051.1"
                     /translation="MQIKLLSDQLISQIAAGEVVERPASALKELLENSVDAGSTQVQV
                     ALQQGGMKLLKVTDNGHGIAKEELELALTRHATSKIQSLEDLEQVGSLGFRGEALASI
                     ASISRTVLTSRQQAASHAWQISAEGTHQQNISPAALDAGTIVEVHDLYFNTPARRKFL
                     KTENTEFGHCEDAFTRVALSRPNVGFLLQHNGKAITRLAANTPQQRITDVLGNEFAAQ
                     SIWLEEESGGLRLWGMTASPTYQRHSRDSQYVFVNGRFVRDKLIAHAIKQAYQDVLHG
                     DKHPAFVLFLELDPSLVDVNVHPAKTEVRFRESQAVHRFIFHSLHKALGKTSAEIQAS
                     QPVQAPFNPFRPTFNMPREQVEMPLQNRPAFGGNYPSNSAGNGAAMAQQFYGQLYGDL
                     ITTETSALGNPPERHVPEDYLNPVSITETVLVPEPAVEPQQYPLGFALGQLHGIYILA
                     QNTQGLVVVDMHAAHERIMYERLKNALNEQNIATQPLLIPMSFYAEKVEVATLQSLQG
                     SDTLHQLGFDLAPLSPTTIAIRAVPSMLQDADVVSLARAVLRDLHEYGASRVLLERQN
                     EVLGTMACHAAVRANRQLTLTEMNALLRDMEATERSGQCNHGRPTWFQVSLGDLDKMF
                     MRGQ"
     gene            2601..4124
                     /locus_tag="VNZ84_08575"
     CDS             2601..4124
                     /locus_tag="VNZ84_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWU67052.1"
                     /translation="MSLSDFQAYLSRFSLARQLHRQHHFYVGPTNSGKTYQALETLRQ
                     AKSGVYLAPLRLLAMEVRDRLMQAGVPCNLVTGEERLMVPGAHHTASTIEMMRPDQAV
                     DVAVIDEIQMLMDPDRGSAWTAALLGAPAKQVFICGANSVTESCTRVLEALNEPYTLT
                     HLQRMTPLVIEEASICGERYHAAKLRKALQPGDAIIAFSRKDVLTLAARIRQWGFQVA
                     TIYGALSPEVRRGESARFANGEAQILVATDAIGMGLNLPIRRIIFANIHKFDGIAPRP
                     LNATEMRQIAGRAGRHGLYDIGYVTVLEDDEQWHLHEMLGCDDTVALFALPIAVGSDD
                     IEALSQKIPTWRLAECVEYLHAQYQRQSGTTVLKSHVTPLQYQQALLIDETAPKLTMQ
                     DKYRLMCAPLGIQVPIARDYFVQCIQAISNYQPRPLPVMPQWLKGQQAEYLEQAELIC
                     QHLSLYSWLGYQYPKIFRDGDLVDEARKKLNAFISRALRVQAGYGKTQREAELGQYR"
     gene            complement(4814..6100)
                     /locus_tag="VNZ84_08580"
     CDS             complement(4814..6100)
                     /locus_tag="VNZ84_08580"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWU67053.1"
                     /translation="MLSINSFADAAEVILANLQQHGNQVQLELLVDQSIKYRVFTLDN
                     PFRVVIDMDDVAISPTLKALPPQLDAQHPYLSKIRIAPFAEHTTRVVFDIRSDIKPLV
                     TDMNAGSSQQRLAISIGPAIGSAEPAVATPVLMPSKQLQDVPPGNNEDKPAEQATPPV
                     TGKEPAPETPAANTSADKPAEKPAVADKPKQAKPGQKIITIAVDAGHGGEDPGARGAN
                     GSHEKNITLAIARKLKQQIDAEENMQAILIRDGDYFVPLGDRVKKARAAKADLFISIH
                     ADSFINSTAKGSSVFALSERGATSASARYLAKKENAVDLIGGVAIDTRDMDLARTLLD
                     LSQTATIHDSIRMGKAVLGRIAKINTLHSKYVEQAAFAVLKSPDIPSILVETAFISNP
                     AEEARLNDDGYQDKLVNAILSGVKSYIATNPSFAKK"
BASE COUNT         1488 a         1623 c         1516 g         1546 t
ORIGIN      
        1 gctgcataaa agatagcttc ttaaacaggt cagccaaagg cccggcttga tgcgggatag
       61 attgcttttc gacttcatgg gcttcaaact cgcccagatc attattcatt ttgaatacag
      121 cttctgcctc atcgtccgca atcacgccat caaagtcaaa cgcaatcctc aattcagtat
      181 ccgcttcatc atcatagact ttggatggta aaaccacgcc tgctggatag tctaaatcga
      241 tcgcgctttg cacatcttct tcattggcac ttaaaaataa tgacgtatta aaggccggaa
      301 tatactcatg cggagacctg cccgtcataa agctggcgcg ggtaatgtcc aacccataat
      361 gcctgatgga tcgaaacacc ctgagccctg tttctggcga gtttctggat aaaagtacca
      421 cttcgaccgg taactctgca gggaacgctt tgttaattgc cagaaacctt ctgataaaag
      481 ggaatgcaac gcccttaggg aaaggcacat caatattgac ttcctgatgc cttcgatatt
      541 cgtctacgcc ttgagtttta tagacctcat gcgactcggt taaatcaaac aaagcgcttg
      601 aggccacgct gatgactaat tttttctcaa tcggataagc cataaactat tattcttatg
      661 tattgtttat agaagcagtt actgcccacg cataaacatt ttatccaaat cacctaaact
      721 tacctggaac caggtaggac ggccatgatt gcattgcccg cttctttcag tcgcttccat
      781 atctcttaat aaagcgttca tttcggtcag tgtgagctgc ctgttggctc tgaccgctgc
      841 gtggcaagcc atggtaccca atacctcatt ttgcctttcc agcagtactc ggctggcacc
      901 atattcatgc aggtctcgca atacggcgcg cgcaagtgac acgacatccg catcttgcaa
      961 catagaaggc accgcacgga ttgcaatagt cgttggggac aagggtgcca aatcaaaccc
     1021 caattgatgc aaagtatcgc taccttgtag tgattgcagc gtggccacct cgactttttc
     1081 agcataaaaa ctcatgggga tcagtaatgg ctgagtggca atattttgct cgttcaaggc
     1141 gtttttcagg cgttcgtaca taatgcgctc atgagcggca tgcatatcga ccaccaccaa
     1201 accttgcgta ttctgggcga ggatataaat tccgtgtaac tgccctagcg caaaacctaa
     1261 tggatattgc tgtggctcta cagctggctc aggaacaagc actgtttcag tgatggagac
     1321 aggattcaaa taatcctctg gcacatgccg ctcaggcgga ttacccaagg cactcgtctc
     1381 ggtcgttatt aaatccccat agagctggcc ataaaactgt tgcgccatgg ctgccccgtt
     1441 acctgcgcta ttagagggat aattaccacc aaaagcgggc cgattttgca aaggcatttc
     1501 cacctgctca cgcggcatat taaaagtcgg ccggaacgga ttaaatggcg cctgtacggg
     1561 ctggctggcc tgaatctcag ccgaggtttt gcccaaggct ttatgcaggc tgtgaaagat
     1621 aaagcggtgc accgcttgtg attcgcgaaa gcgcacttct gttttggccg ggtgtacatt
     1681 aacatccacc agtgacggat ccagctccag aaacaataca aaagccggat gcttatcgcc
     1741 atgcaataca tcctggtagg cctgtttaat cgcgtgtgca atcagtttgt ctcgcacaaa
     1801 acgtccattc acaaacacat actggctgtc acggctgtgg cgctggtagg tgggagaagc
     1861 cgtcatgccc cacaggcgca gccctccgct ctcttcttcc agccaaatgc tttgcgcggc
     1921 aaactcattc ccgagcacat cagtaatacg ttgctgtgga gtattggccg ccaggcgcgt
     1981 aatggctttg ccgttgtgct gtaataaaaa gcccacattt ggacgtgata aggccactcg
     2041 ggtaaacgca tcctcacaat ggccaaattc ggtattctcg gttttaagaa acttgcggcg
     2101 tgccggggtg ttaaagtaca aatcatgcac ctcaacgatg gtgccagcat ctaatgccgc
     2161 aggactgata ttctgctgat gtgtgccttc agcggaaatt tgccaggcat ggctggcagc
     2221 ttgctggcga ctcgtcagca ccgtgcgtga aatggaggca atgctggcca gagcctctcc
     2281 cctgaaaccc aaactaccga cttgctccag gtcttccagg ctttggattt tgctggtggc
     2341 atgccgcgtc aatgccagct caagctcttc tttcgcaatg ccgtggccgt tatcagtgac
     2401 tttcagcaac ttcatcccgc cctgttgcaa ggcaacctgt acttgtgtac tccccgcatc
     2461 cacactgttt tcaagcagct ctttgagcgc agaagccgga cgttcaacaa cttcgccagc
     2521 ggcaatttga ctgattaatt gatcagataa gagtttaatt tgcatctcgt cattctaccc
     2581 gttttgctag aatggaggcc atgtcgcttt ccgattttca agcctatctc agccgcttta
     2641 gcctcgcccg ccagttgcat cgccagcatc acttttatgt tggcccaacc aactccggta
     2701 aaacttacca ggcactggaa acattaaggc aagccaagtc aggcgtctat ctggctccat
     2761 tacgtctgtt agcgatggag gtgcgtgacc ggctcatgca agccggcgta ccgtgcaacc
     2821 tggtcacggg tgaagagcgt ctcatggtcc ctggcgcgca tcataccgcc tcgaccatag
     2881 aaatgatgcg tccagatcaa gccgtcgatg tcgcggtgat tgatgaaatc cagatgctga
     2941 tggacccgga ccgcggtagt gcctggaccg ctgctttgct cggcgcaccc gcaaagcagg
     3001 tatttatctg tggtgccaat agcgtgacag aaagctgcac ccgtgtgctg gaggctctca
     3061 atgagcctta taccctcacc cacttgcaac gcatgacgcc attggtgatc gaagaggcca
     3121 gtatttgcgg ggaacgttac catgcggcca agttacgaaa agctttgcaa ccaggagatg
     3181 cgattattgc atttagccgc aaagatgtgc tgaccttagc cgcccgtatc cgtcaatggg
     3241 gctttcaggt cgccaccatt tatggcgccc tgtcacctga ggtcagacgc ggcgaatctg
     3301 cacgctttgc caatggtgaa gcccagatac tggtcgcgac agacgccatt ggcatgggct
     3361 tgaacctgcc tatccgccgt atcatttttg ccaatatcca caaatttgac ggcatcgccc
     3421 cgcgcccact caatgcgact gaaatgcgcc agattgccgg gcgcgcaggc cgccatggtt
     3481 tatacgatat tggttatgtc accgtgctcg aagatgacga gcaatggcac ctgcatgaaa
     3541 tgctgggctg tgatgacacc gtcgccctct ttgccctacc tattgcagtt gggagcgacg
     3601 atattgaagc actgagccag aaaataccaa cctggcggct ggcagagtgc gtggaatatc
     3661 tgcatgcaca ataccaacgc caatccggca caacggtact gaagtcgcat gtcacgccct
     3721 tgcagtatca gcaggcatta ttgattgatg aaacagctcc caaactgaca atgcaagata
     3781 aatacaggct gatgtgtgcc ccactgggga tacaggtgcc gattgcacgg gattattttg
     3841 tgcaatgcat acaagccatc tccaactatc agcctcgccc cttgccggtg atgcctcaat
     3901 ggttgaaagg tcaacaggcc gaatatctag aacaagcgga actgatttgc cagcacctga
     3961 gtttatatag ctggctggga taccaatacc ccaagatttt tcgggatggc gatttggtcg
     4021 atgaagcacg caaaaaactc aatgcgttta tcagccgggc gttacgggtg caagcggggt
     4081 atgggaagac gcagagagaa gcggaattgg ggcaatatcg ttaatatact ttctgtttct
     4141 ggtagctttt tcgcctctcg gcgaccattc tttcttatgc ttgtccataa gaaagaaggc
     4201 aaagaacaag acaccctagc ttccgcttgt ttcctccggt gctctccaaa acaagcggtc
     4261 acgaaactcg acctggcagc tcacaaaatg cgtgagctgc tgcgggactc gaacagtcgc
     4321 tcccttaccc cttgtttagg ctgcgctact cggcgcgaca gataggggcc cgaaaagcta
     4381 tgagcgccac cgtttgggtc taaaaagaaa agaatttgag tttttgaacg gttcaaattt
     4441 caacagtgtg cgtttggcga gacaaatccc atcccatcac gctgagtagc gcaggcgaag
     4501 tgggagtttt cggccatcgg ctgttcgagt tccgcaacaa gtcacgaagt gtgtggcttg
     4561 tcaggtcgag tttcgatggc cgcccacttc gttgataact aagactgcgc agcagtctgt
     4621 tacggcgtag gctaactcgc gtagcgatgt taagcgcagc ggccgtagcc aacaacgcaa
     4681 ggcagccgaa ggacgtgttg gctgggtgcc gtcttctttg gttactttct gtggggcaag
     4741 caacagaaag taacttgccg agaggcgaaa agatcttatc taattatcga gttacttttc
     4801 aaccaataca gacttacttc ttagcaaatg atggattcgt cgctatatac gatttcacac
     4861 ccgataaaat cgcattcacc aacttatcct ggtaaccatc gtcgttcaac ctcgcctctt
     4921 cagccggatt actgataaac gctgtttcga ccagaatgga tggaatgtcc ggtgatttca
     4981 acactgcaaa cgccgcttgt tccacatact tggaatgtag ggtattaatc ttggcaatgc
     5041 gccccagcac agctttaccc atacgaatac tgtcatgaat cgtcgccgtc tgtgataagt
     5101 cgagcagcgt acgtgccagg tccatgtcgc gtgtatcgat cgccacaccg ccaatcaagt
     5161 ccaccgcgtt ttcctttttc gccaaataac gcgcgctggc gctggttgca ccgcgctcgg
     5221 acaaggcgaa tactgatgaa cctttagcgg tgctgttaat aaaagagtcc gcatggatgg
     5281 agataaacag gtctgccttg gcggcacgcg cttttttcac acggtcaccc aatggcacaa
     5341 aataatcgcc atcgcggatc aggatcgctt gcatattctc ttcagcatca atttgctgct
     5401 tgagtttgcg cgcaatcgcc agggtgatgt ttttttcatg gctaccgttc gcccccctgg
     5461 cgccggggtc ttcaccacca tgccctgcat ccaccgcaat cgtaatgatc ttttggcctg
     5521 gtttagcttg cttgggttta tcggcaacgg ccggcttttc tgcaggttta tcggcgcttg
     5581 tattggcagc tggcgtctct ggcgcaggtt ctttacccgt tacaggaggc gttgcttgct
     5641 ccgcaggttt gtcttcattg ttgcctggcg gtacatcctg taattgtttg gaaggcatca
     5701 acaccggggt ggcaaccgca ggttctgcac taccaatggc tggcccgatg ctgattgcca
     5761 gccgctgttg cgagctgcca gcgttcatgt cggtgaccag gggtttaata tctgacctga
     5821 tatcaaaaac cacccgggtt gtgtgctcgg caaagggagc aatacggatt ttggacaagt
     5881 aaggatgctg tgcatccagc tgtggcggca atgccttgag tgtggggcta atggcaacat
     5941 cgtccatgtc tataactaca cgaaatggat tatccagcgt aaacacgcga tatttaatgg
     6001 actggtcaac cagcaactct aattgcacct ggttaccatg ctgttgcaaa ttagccagaa
     6061 tcacttccgc ggcatcggca aaactgttta tggatagcaa gactgtcagc agccatatcc
     6121 taaaaaaatt tcgagttact ggcataatcc ttccaacgcc agcggatgct gac
//
LOCUS       DATMSK010000257         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_115339,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000257 DATMSK010000000
VERSION     DATMSK010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115339"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..353
                     /locus_tag="VNZ84_08585"
     CDS             84..353
                     /locus_tag="VNZ84_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67054.1"
                     /translation="MKTTNKLAAAALLGLFTASVMTASPAFAGEDSKSSCNGKDNAKM
                     EKANCKTADGKEKHACKGKNSCKGQGGDGKNACKGHGSCATDGSK"
     gene            427..2082
                     /locus_tag="VNZ84_08590"
     CDS             427..2082
                     /locus_tag="VNZ84_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HWU67055.1"
                     /translation="MQIEHPSLSLGFGLGLRPKHYEYIFEHQPKVDWFEIISENFMDT
                     DGKPKRNLARIKEQYPLVMHGVSMSIGSMDPLNSEYLTKLKALMDWVNPAWISDHLCW
                     TGVAHKNTHDLLPLPYTEESLKHIVRRIQQVQDRLGRRIALENPSTYLEFKHSSMPEA
                     EFIAAMAKEADCHLLLDVNNVYVTCFNHRLDPKTYLDALPLERVIQMHLSGHSNKGHY
                     IVDTHDDHVIDEVWNLYKYVVHKAGRIPNTMIEWDDRIPEFPVLHAELDKARAAARHA
                     AEFTLPQIELVDDVLVANHDVTLLEAQNHMQQAVMQGDRFDSVPTQWIRAKNAFAPHE
                     QLSVYINAYRYRLYDVVAEDYPVLKHYLTEKKFSDLMWAFVGEVLPDHFNIGRFALKL
                     PAFIQQALPDDAFAHALCQLETAVAQMTDPMETEALNESHLQGLTAETILDLTLYPRQ
                     ALVLMQFDDQVNAYYQAVMDDDEAVDVMHEPVYLAVFRHEDVVWRMELEAQEFALLSR
                     LFGGATIGETLTDVQESEQYNITAYFSKWMRNGLLASHHYEYL"
     gene            2100..2552
                     /locus_tag="VNZ84_08595"
     CDS             2100..2552
                     /locus_tag="VNZ84_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWU67056.1"
                     /translation="MNAILNILIWWPKRIAAHFEWAGPLVARIVVGYTFMLTGWGKLM
                     NLPAIIENFQSWGIPYPEVLTPFVAGWEFLGGLGLIIGLMTRICGGALAVVMVVAIIS
                     AKLADIDSLETLLGFEEATYFAVFTWLAICGAGKASLDYFLERRAPKQ"
     gene            complement(2604..>2815)
                     /locus_tag="VNZ84_08600"
     CDS             complement(2604..>2815)
                     /locus_tag="VNZ84_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HWU67057.1"
                     /translation="NLFAQLGLPSSDEEIEQFIQTHSHLASYITLADAPYWTPAQSTF
                     LKEEILKDADWAEVIDQLNARLHAK"
BASE COUNT          693 a          656 c          767 g          699 t
ORIGIN      
        1 gagtttgtaa cccatcagac taaaggtggc agtacaagtt tactgattca ttttttcgtt
       61 gattgttact tttaggagat gttatgaaaa ccactaataa attagccgct gccgcacttt
      121 tgggcctgtt tactgcttcc gtgatgacag cttcaccagc gtttgcaggc gaagatagca
      181 agtccagctg caacggcaag gacaatgcca agatggaaaa agccaactgc aaaacagcag
      241 atggcaaaga gaagcatgcg tgtaaaggca aaaactcctg taaaggccag ggcggtgatg
      301 gcaaaaatgc atgtaaaggt cacggttcct gcgcaacgga tggtagcaag taatcggacc
      361 attctcttaa ccagactcaa aacggcgggg ggtatacccc tgctgttttg agaaagatga
      421 gctgacatgc agatagagca tccatcactt tccctcggtt tcgggctagg gctgaggcct
      481 aagcattatg agtatatttt tgagcatcaa cccaaggtgg attggtttga aattatttcc
      541 gaaaacttta tggataccga tggcaaacca aaacgcaacc tggcgcgcat caaggagcag
      601 tatccgctgg tgatgcatgg cgtatccatg tcgattggca gcatggatcc attgaactcg
      661 gagtatctga ccaagctgaa agcgctgatg gattgggtaa acccggcctg gatttctgac
      721 cacctgtgct ggaccggtgt ggcgcataaa aatacgcatg acttgctgcc gctgccttat
      781 accgaagaat cgctgaagca tattgtgcga cgcatacagc aagtgcaaga ccgcttaggg
      841 cgcaggattg ccctggaaaa cccgtcgacc tatcttgaat ttaaacattc cagcatgcca
      901 gaagcggaat ttattgcggc catggcaaag gaggctgact gccacttact gctggacgtg
      961 aataatgtgt acgtgacctg ttttaaccac cgactggacc cgaaaaccta cctggatgca
     1021 ctgcctttgg agcgtgtgat ccagatgcat ctttccgggc acagcaataa aggacattac
     1081 attgtggata cccacgatga ccatgtgatt gacgaggtgt ggaatctcta caagtatgtg
     1141 gtacacaaag ccgggcgtat cccgaatact atgattgaat gggatgaccg tatcccagag
     1201 tttcccgtgc tgcatgcaga gctggataag gccagagcag ccgccaggca tgccgccgaa
     1261 tttaccttgc ctcagattga actggttgac gatgtgcttg tggctaatca cgatgtgacg
     1321 ctgctggagg cgcaaaacca tatgcaacag gcggtgatgc agggcgacag gtttgatagc
     1381 gtgccaacgc aatggatacg tgccaaaaat gcttttgcgc cgcacgagca gctctcggtg
     1441 tatatcaatg cttaccgcta tcgcttatac gatgtggtgg cggaggatta tccggtattg
     1501 aagcattacc tgacggaaaa aaaattctcg gacctgatgt gggcatttgt gggcgaggtg
     1561 ttgccggatc actttaatat tggccgtttt gccctcaagc tgccggcgtt cattcaacaa
     1621 gccttgcccg atgatgcgtt tgcgcatgcc ttgtgccagc tggaaacggc cgtggcacaa
     1681 atgacggacc ccatggaaac cgaagcgctc aatgaatcgc atctgcaggg actgacggct
     1741 gaaaccatcc ttgatttgac gttgtatccc cggcaagcac tggtgttgat gcagtttgat
     1801 gaccaggtga atgcctatta ccaggccgtg atggatgacg acgaagctgt ggatgtgatg
     1861 catgagccgg tgtacctggc cgtcttcagg catgaggatg tcgtctggcg catggagctg
     1921 gaggcgcaag agtttgcgtt gttatccagg ttgtttggcg gtgcgaccat aggcgagacg
     1981 ctgacggatg tgcaggagtc ggagcaatac aacatcacgg cctatttttc aaaatggatg
     2041 cgcaatggtt tactggcatc ccatcattat gagtatctat aaactgaagg ggaacaaaca
     2101 tgaacgcaat cttgaatata ctgatctggt ggcctaaaag aatcgctgcc cattttgaat
     2161 gggctggccc gttggtggcg cgcattgtcg tgggctacac gtttatgctg accggctggg
     2221 gcaagctgat gaatctgccc gccatcattg aaaattttca aagttggggc atcccttacc
     2281 cggaagtcct gacgcctttt gtcgccggct gggagttttt aggcggcttg ggcctgatca
     2341 ttggcctcat gacaagaatt tgcggtggcg cattggcggt ggtcatggtg gtcgccatta
     2401 tttcggccaa actcgctgac attgattccc tggaaacctt gttgggcttc gaagaggcaa
     2461 cctattttgc cgtcttcacc tggctggcga tttgcggggc aggcaaggct tccctggatt
     2521 atttcttaga gcgccgggca cccaaacaat agatgccccc agcacgtcgc ctgtttcatg
     2581 cgctaaaaca gacgacgtgg gttttatttt gcatgcaacc ttgcattaag ctggtctatc
     2641 acttctgccc aatcggcatc tttcaaaatt tcttccttta aaaaagtcga ctgcgctggt
     2701 gtccagtaag gggcatctgc cagcgtgatg tagctcgcca aatggctgtg cgtttgaatg
     2761 aactgctcga tttcttcatc actggatggc aagccaagtt gcgcaaacag gttgt
//
LOCUS       DATMSK010000258         1863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_68413,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000258 DATMSK010000000
VERSION     DATMSK010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68413"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1785
                     /locus_tag="VNZ84_08605"
     CDS             61..1785
                     /locus_tag="VNZ84_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HWU67058.1"
                     /translation="MLKLKLMLGFWLASLTRKLKNNFYLYLALLFSVVILLDAAVFHV
                     GQNMRDKAFDLMVKNRIIKPAADASIVIVDINEASLDAMAKEYGRWPWPRQVIGELVE
                     NIQQQQPKAIVFDIVFSDADIYNPDSDSYFNDVITECQHCFFPLLRLDTSQDHQSQLK
                     YAQIPSLTHTPDADMQATFAAIPPYFKGAWKAGSLGTHNVYPDNDGVVRQYRMRHQDR
                     GWTLPALPLVVANAESDNSLNTPDEMLLNWRGKPFTYAYVSFSDVYRDITSAHPKRAS
                     NEFKDKIVIIGSTAPALFDIKPTAMDKQFPGVEILATALDNSLHQDYLKVWRGKLPYI
                     LLSFLLVWLTTLAFYLKVDRDRFNGIFTGSQIGLLVLSYLAINLFHTYLDLAAPIFWA
                     VAYFGIAKIYALATDRALQRWLAFGLSSSSHELEVLLMPIAIESRETLGDAVMKRIRR
                     EIEQACRAPTQIDILTGTQSGIWGLFGDMVMVSWQYAPETTYREAARQDAQQLAAQLQ
                     SLLNKLSLPGLPVVRYAMHQGNIANNASQLAAQWRVLFAQAMIQLEMADVSNTENTTP
                     QTKTENNA"
BASE COUNT          463 a          535 c          466 g          399 t
ORIGIN      
        1 gtcaccctgg atgacatcaa gatagtcaag ataataagcg gtctataaca agggagcgat
       61 atgttaaaac tcaaattgat gctgggcttt tggctggcca gcctgacccg caagctaaaa
      121 aataactttt acctgtatct ggccttgctc ttcagtgtgg tgatcctgct cgatgccgcg
      181 gtcttccatg tcgggcagaa catgcgtgac aaagcgtttg accttatggt caaaaaccgc
      241 atcatcaagc ctgccgctga tgcctctatt gtcattgtcg acatcaatga agccagcctg
      301 gatgccatgg ccaaggagta tggccgctgg ccgtggccgc gccaggtgat cggcgaactg
      361 gtggaaaaca tccagcagca acaacccaaa gccatcgtat ttgatatcgt gttttcggat
      421 gcagacatct ataacccgga cagcgacagc tatttcaatg atgtcattac cgagtgccag
      481 cattgtttct tcccgttgct acggctggac acaagccagg atcaccagag ccagttgaag
      541 tatgcacaaa tccctagcct gacgcacacg cctgacgcag acatgcaggc gacatttgcc
      601 gccattcccc cttatttcaa aggcgcctgg aaagctggca gcctgggcac ccataatgtg
      661 tacccggata acgatggtgt ggtgcgccaa taccgcatgc gccaccagga tagaggctgg
      721 accttacccg cgctgccgct ggtggttgct aacgccgaga gtgataacag cctgaatacg
      781 ccggacgaga tgctgctcaa ctggcgcggc aaaccgttta cctacgccta tgtgagcttt
      841 agcgacgttt accgcgacat taccagcgcc caccccaaac gcgccagcaa cgaattcaag
      901 gacaagatag tcattatcgg ctccaccgca ccagccctgt ttgacatcaa gcccaccgcc
      961 atggataagc agttcccagg tgttgaaatc ctcgcgacag cgctggataa cagcctgcac
     1021 caggattacc tcaaagtttg gcgcggcaaa ctgccttaca tcctgttgag ttttttactg
     1081 gtatggctga ccaccctggc gttctatctc aaggtggacc gcgaccggtt taacggcatt
     1141 tttaccggca gccagatcgg cttgctggtg ctgtcttacc tcgccatcaa cctgttccat
     1201 acctacctag acctggcagc ccctatcttc tgggcagtgg cttacttcgg cattgccaaa
     1261 atctatgcgc tggcaacaga ccgcgccttg caacgctggc tggcatttgg cctgagttcc
     1321 agcagtcacg agttggaagt gctgctgatg ccgattgcca tcgaaagtcg cgaaaccctg
     1381 ggtgatgccg tcatgaagcg cattcgccgt gaaatcgagc aagcctgccg cgcaccaacg
     1441 cagattgaca ttctcaccgg cacccaaagc ggcatctggg gcctgtttgg cgatatggtc
     1501 atggtcagct ggcaatacgc gccggaaacc acctaccgcg aagcagcacg gcaggacgca
     1561 cagcaactcg ccgcgcaatt acaatcttta ctcaataagc tgagtctacc cggactgcct
     1621 gtggtgcgtt atgccatgca tcagggcaat attgccaaca acgcatcaca actggctgca
     1681 cagtggcgcg tattgtttgc gcaagccatg atacaactgg aaatggcaga tgtatccaat
     1741 acagaaaata caacgccaca aactaaaacg gagaacaacg catgatcaaa aaaagcctat
     1801 tctggttact gacggcattc gcatgcagtc aggcagccgc agcagaaacc ggggtcgccc
     1861 tca
//
LOCUS       DATMSK010000259         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_137900,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000259 DATMSK010000000
VERSION     DATMSK010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137900"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..401
                     /gene="flhD"
                     /locus_tag="VNZ84_08610"
     CDS             81..401
                     /gene="flhD"
                     /locus_tag="VNZ84_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HWU67059.1"
                     /translation="MNMQNDLLAEIKDANLNYLMLAQQLIRADKATAIFRLGINKEIA
                     DLLENLTNLQLLKLCNTNMLLTRFRFDDSEILGMLTSYAKDPAQAHLHTAVLMSAQAA
                     ESTV"
     gene            454..1020
                     /gene="flhC"
                     /locus_tag="VNZ84_08615"
     CDS             454..1020
                     /gene="flhC"
                     /locus_tag="VNZ84_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HWU67060.1"
                     /translation="MQKKSVVNEAEQIQLAMQMIELGARLQLLESQTTLSRERLIKLY
                     KEMKGVSPPKGMLPFSTDWFLTWQPNIHSSIFYNIYRFMVDYAGVDGIQAIIKAYTLY
                     MQQVQNPDPAQQDEPVLSLTRAWTLVRFIESKMLTTKNCHCCGGQYIVNSYDLNQNYV
                     CNLCHVPSRAGKTKKAKELSYKLVSMSA"
     gene            1064..1915
                     /locus_tag="VNZ84_08620"
     CDS             1064..1915
                     /locus_tag="VNZ84_08620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU67061.1"
                     /translation="MHSVKLSAEALPSQAWMQRFLPKRLWAQALLIQCVSMLLITGLA
                     PGVINSVFAFELRSLGVRLDTIWFWLLPHVCLTSVMAKRAKMPAWWRWIHVIFPVAVV
                     FMQQVALPPGVYFAGFIMTLALYWSVHNTRVPFYPSFPSTWRAMHHILEQHAGDRSLQ
                     VLDIGSGIGDIAMFLAKQRIHDEVSGIEIAPLPWAISAVRAIFSGTSVRFTLGDYRQL
                     DFAALDVVFAYLSPAVMTDVWQKVQHEMRPGSLFVSSEFPVAELAADRIIYPSAQAPA
                     LYIYHIR"
     gene            2127..2987
                     /gene="motA"
                     /locus_tag="VNZ84_08625"
     CDS             2127..2987
                     /gene="motA"
                     /locus_tag="VNZ84_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HWU67062.1"
                     /translation="MFVIIGYVVVCGMIFGGYALAGGHLGALWQPLEVMMIFGGAFGA
                     FIVGNDSKAIKATMKSIPTLFKGSKYNKQLYMDLLALLFEILTKIRKDGLMSIEKDIE
                     DPESSALFSKYPSIQHDHHLIEFICDYLRLMVSGNMDAFQVENLMDNELETHHMEGHV
                     PAHCIAKLGDGLPAFGIVAAVMGVVHTMENVALPPAELGILIAHALVGTFLGILLAYG
                     FIGPLSGLLEQKLEESSKSFQTVKVVLLASLNGYAPPIAVEFGRKVLYSTERPGFTEL
                     EEHVKQTKSK"
     gene            3015..3947
                     /gene="motB"
                     /locus_tag="VNZ84_08630"
     CDS             3015..3947
                     /gene="motB"
                     /locus_tag="VNZ84_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWU67063.1"
                     /translation="MAEEHIAPIIVKKIKKNGGGHHGGAWKIAYADFVTAMMAFFLLM
                     WLLGSTSKAQKEGIAEYFQTPLHMVLMGPSLGQSDSALRNTGGKDVTKKQGQVKPVEG
                     DPGKLKTVKIEDVKEALKQAEKVKLEALKAKIEQAIEDNPTLQQFKSQLKLDITSEGL
                     RVQIIDEQNRPMFENAKAELQPYAKTILREIGKTLNGVDNKISLSGHTDAAAYANGDH
                     GYSNWELSADRANASRRELIAGGMDETKLMRVVGLGSAIMMDKENPFNPINRRISLIV
                     MNKETEEELLKDNEQISVSEQDKSQVEALQKKPE"
BASE COUNT         1080 a          925 c          979 g         1005 t
ORIGIN      
        1 gttgctgttc atgattgcgc gcaaagtacg cctaacgctc aggcgtactt aaatcaacgc
       61 aaaaaaacgg agaaaagatc atgaacatgc aaaacgattt actcgctgaa atcaaagacg
      121 ctaacctgaa ctacctgatg ttggctcaac aattgatccg cgctgacaaa gccactgcta
      181 ttttccgcct tggtattaat aaggaaatcg cagacctgtt ggaaaacctg actaacctgc
      241 aactcctcaa actctgtaac accaacatgt tgttgactcg cttccgcttt gatgacagcg
      301 aaattctggg catgttgacc agctacgcta aagatcctgc gcaagcgcac ttgcacactg
      361 cggtattgat gtccgcgcaa gcagcagagt ccacagttta agccagttga tcaaaattca
      421 atctgtcgat aaagaataga aaagagggtt atcatgcaaa agaagagtgt cgtaaacgaa
      481 gcagaacaaa ttcaactggc gatgcaaatg attgaattgg gcgcccgcct gcaattgctg
      541 gaatctcaaa caaccttgtc acgtgagcgt ttgattaagc tgtacaaaga gatgaaaggg
      601 gtttctccac ccaaaggcat gttgccattc tctactgact ggtttttgac ctggcagcca
      661 aatattcact cttctatttt ctacaacatt tatcgtttca tggttgatta cgctggcgta
      721 gacggtattc aggccattat caaagcctat accctgtata tgcagcaagt acaaaaccct
      781 gatccagccc aacaagatga gccagtattg tcactgaccc gtgcctggac cctggtgcgt
      841 ttcattgaaa gcaaaatgct gacaaccaag aactgccatt gctgcggtgg ccaatacatc
      901 gttaacagct acgacttgaa ccagaactat gtgtgcaacc tgtgtcacgt accttcacgt
      961 gcgggtaaaa ccaaaaaagc gaaagaactc agctacaaat tggtttccat gtctgcttaa
     1021 gtggtaaggc agggaagcgt ttcctgcaaa atgaaggatt agcatgcact cagtaaaact
     1081 ctcggcagag gcgttgccat cgcaagcatg gatgcagcgt ttcttgccta aaagattatg
     1141 ggctcaggcc ttactgatcc agtgtgtgag tatgctgttg atcaccggcc tggctcctgg
     1201 cgtgatcaac agcgtatttg cgtttgagtt acgtagtcta ggcgtccgcc tggatactat
     1261 ctggttctgg ctgctaccac acgtttgctt gacgagtgtg atggcaaaaa gagcgaagat
     1321 gccagcctgg tggcgctgga tacatgttat tttcccggtg gctgtcgttt tcatgcaaca
     1381 ggttgcgtta ccgcctggtg tgtactttgc cggttttatc atgacactgg ccctttactg
     1441 gtcagtccac aatacccgcg ttccttttta tccgtctttt ccatcgacct ggcgcgctat
     1501 gcaccatatc ctggaacagc atgctggcga ccgttccctg caagtattgg atattggcag
     1561 tggcatcggt gatattgcca tgttcctcgc caagcagcgc atccatgatg aagtgtctgg
     1621 cattgagatt gcacctttac cgtgggcgat cagtgcagtg cgtgccattt tttccggcac
     1681 cagcgtcagg ttcaccctgg gcgactaccg tcagctggat tttgcagcat tagatgtcgt
     1741 attcgcttac ctttccccgg ctgtcatgac cgatgtctgg caaaaagtgc aacatgaaat
     1801 gcggcctggc agtttatttg tcagctccga atttccggtt gcagagctgg cagccgaccg
     1861 catcatttac ccttcagcgc aggcccctgc gctgtacatc taccatatcc gttgaatcac
     1921 ataccttaaa atcgcaaccc gatgtaacgc gctttgctgg atataccttc aggccgcatc
     1981 caggcttcaa atactttttg actttactca cgcaatgaat acttagccaa gtaatttggc
     2041 tggctaaatc ccttttattt ggaaaaatga attgcgtagc agattgatat gctgataaca
     2101 gttaaatgct aaaagcggag tagagcatgt ttgtcatcat tggttatgta gtcgtgtgtg
     2161 gcatgatttt tgggggatat gccctggcag gcggccattt gggcgcattg tggcaaccac
     2221 tggaagttat gatgattttc ggtggcgcat ttggtgcctt tattgtcggt aatgacagta
     2281 aagcgatcaa agcaacaatg aaatccatcc caaccttgtt taaaggctcc aaatacaaca
     2341 aacagcttta tatggacttg ctggccctgt tgtttgagat cctgactaaa atccgcaaag
     2401 acggcctgat gtcgattgag aaagacatcg aagaccctga atcaagtgca ttgtttagca
     2461 aatatccttc tatccagcac gaccatcacc tcatcgaatt tatctgtgat tacctgcgtc
     2521 tgatggtgtc cggcaatatg gatgcgttcc aggtggaaaa cctgatggac aatgaactgg
     2581 aaacccacca tatggaaggt catgtgcctg cacattgtat tgccaagctg ggcgatggtt
     2641 tgcctgcgtt tggtattgtg gctgcggtga tgggcgtagt acacaccatg gaaaacgtgg
     2701 cgttgccacc tgcggaactg ggcatcctga ttgctcacgc cctggtaggg accttcctcg
     2761 gtattttact ggcttatggt tttattggcc cattgtccgg tttgctggaa cagaaactgg
     2821 aggagtccag caaaagcttc cagaccgtca aagttgtatt acttgccagc ctcaatggat
     2881 atgccccgcc aattgcagtg gaatttggcc gtaaggtatt gtactcaacc gaacgtcctg
     2941 ggtttaccga gcttgaagag cacgtcaaac agactaaatc caagtaatca catcaagacc
     3001 gccaggagag cgtcatggca gaagaacaca ttgccccgat tattgttaag aagatcaaga
     3061 agaatggcgg gggccatcac ggtggtgcct ggaagattgc ttatgccgac tttgtgaccg
     3121 caatgatggc attttttcta ttgatgtggt tattaggctc aacttccaaa gcgcaaaaag
     3181 aaggcattgc tgaatatttc cagacccctt tgcatatggt gttgatgggg ccatctttgg
     3241 gtcaaagcga ctccgcgctg agaaatactg gcggtaagga tgtcaccaag aaacaaggtc
     3301 aggtgaaacc ggtagagggt gatccgggca aactcaagac ggtgaaaatt gaagacgtta
     3361 aagaagcatt aaagcaagcc gaaaaagtaa aactggaagc actgaaggcg aaaatcgagc
     3421 aggccattga agataacccg acgctgcaac agttcaagtc acaacttaag ctggatatca
     3481 ccagtgaagg cttgcgtgtc cagattattg atgagcagaa ccggcccatg tttgaaaatg
     3541 caaaagccga attgcagcct tatgccaaaa caattctgcg cgagattggt aaaacactca
     3601 atggggtaga taacaaaatc agcctgtctg ggcatacgga tgctgcggcg tatgccaatg
     3661 gcgaccacgg ctacagtaac tgggagttgt ctgcagaccg cgctaacgcg tcccgacgtg
     3721 agctgattgc cggcgggatg gatgaaacca agttgatgcg tgtcgttggc ctgggctccg
     3781 ccatcatgat ggataaggaa aaccccttta atccgatcaa ccgccggatc agcctgatcg
     3841 tgatgaataa agaaactgaa gaggagttgc tcaaggacaa tgagcaaatt tccgtcagtg
     3901 agcaggataa atcgcaagta gaagcgttac agaaaaaacc ggaatagcct acgcagtaga
     3961 aagtagctag ccgttaacgt acaagaaat
//
LOCUS       DATMSK010000260         3947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_421071,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000260 DATMSK010000000
VERSION     DATMSK010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421071"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VNZ84_08635"
     CDS             complement(<1..1133)
                     /locus_tag="VNZ84_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU67064.1"
                     /translation="MSLVLLSLYAVAMINAGRERFAAICMAHGLAAIIALICALDVPI
                     PGVVRSVHMYFLPVAAGASLVFRKENIYLRIILPLLLLMGCLVFGASDIGITQAEWVA
                     DPSVRAVGAWINNLAALGVLGVALIVMQSDITLRSELFTDMREALAKGHFCLFYQPQV
                     DAQGNVFGAEALLRWEHPIRGMIPPGLFIAAAEETGLILPIGSWVLKKACAQLVSWSG
                     DPIKSHLSISVNISASQLRQPDFVQEVIEIVTRSGVRPGQLKLELTESVFAKEVDMTV
                     QKMRALRDFGISWSLDDFGTGYSSLNYLKHLPFEQLKIDQSFVRDLLTNESDKAIVET
                     LVALSKSMNMLLIAEGVETLEQLDYLLDKGCTKYQGYLFSKPLP"
     regulatory      complement(1532..1616)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1798..1882)
                     /locus_tag="VNZ84_08640"
     tRNA            complement(1798..1882)
                     /locus_tag="VNZ84_08640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Leu,seq:caa)
     gene            1937..2977
                     /gene="queA"
                     /locus_tag="VNZ84_08645"
     CDS             1937..2977
                     /gene="queA"
                     /locus_tag="VNZ84_08645"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWU67065.1"
                     /translation="MRTLDFDFYLPPHLIAQHPLPNRSESRLLEVNAANNTIQDNDFR
                     HITDHFESGDVLVLNDTKVIKARLFGQKATGGQIEVMIDRVLSAQEAYAQIKSSRSPK
                     AGSLLHLSGDYQAEVLGRHADMFHIRLLGNESWYDFLEQYGQLPLPPYIEHQADAADD
                     TRYQTVFAKHAGAVAAPTAGLHFDDALLQALMNKGVHVRYVTLHVGAGTFQPVKVDNI
                     SEHHMHSEVYHLPEDTIQTILDARASGKRITAVGTTSLRTLESAAHAGKLFAHHGETS
                     IFITPGFEFKLVDRLITNFHLPKSTLMMLVSAFGGYDLMRQAYSHAIEKAYRFFSYGD
                     AMLITRASSSKS"
     gene            3037..3906
                     /locus_tag="VNZ84_08650"
     CDS             3037..3906
                     /locus_tag="VNZ84_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67066.1"
                     /translation="MQFNNTLSGLALFCLAFGAHADILPEAEVGIKIPLTKKPTTRPM
                     GVAYLPSNQHYYIADGGLAPMGSEFEAPISKSEVHAFTSNGQYVNSAKPGYDNRSLYY
                     NPNSGKLEIITYNISSAVGFSPNTGIYSLDVDDKGELLATSGEVAQFNPAFGDAGTMP
                     SYDPASKHYYAKQDRGNIVFVVDAKQREKLQEIKLDFAKAQVAHDDVSDHFIAFTGVA
                     GNELALLDVDHKAVLIFDLNGQYVGKSALPATMKLRSQNHFNGLGYTNGMMFVYHEPE
                     GEFGTYYGFKVVK"
BASE COUNT         1108 a         1053 c          853 g          933 t
ORIGIN      
        1 ggtaaaggct tgctgaaaag atacccctga tatttagtac agcctttgtc gagtaaataa
       61 tctaattgct caagcgtttc taccccttcc gcaatcagta acatattcat acttttactc
      121 agcgccacca aggtttctac aatcgcctta tcgctctcgt tcgtcagcaa gtcgcgcaca
      181 aaagactggt ctattttcaa ttgctcgaat ggcaagtgtt ttaaataatt gagtgaagaa
      241 tagcctgtgc caaagtcatc cagggaccaa ctgataccaa agtcgcgcaa ggcccgcatt
      301 ttctggacag tcatgtctac ctctttcgca aacacacttt ccgtcagttc aagcttgagc
      361 tgccctggac gaacaccact gcgcgtcaca atctcaatca cttcctgaac aaagtcaggt
      421 tgccttaatt gcgaggcact aatattcact gaaatactca ggtggctttt aattgggtct
      481 cctgaccagc ttactaattg ggcacatgct ttttttaaaa cccagctgcc gatcggcaga
      541 atcaatcccg tttcttcagc ggcggcaata aacaatcccg gtgggatcat ccccctgata
      601 ggatgctccc aacgtagcaa ggcttcggcc ccaaaaacat tgccttgcgc atccacctgc
      661 ggctggtaaa acaaacaaaa atgtccttta gcaagcgcct ctcgcatgtc ggtaaacagc
      721 tcgctgcgca aggtgatgtc agactgcatc acaatcaacg caacccctaa caccccgagc
      781 gcggccagat tattgatcca ggccccaaca gcccgaacac tggggtcagc cacccactca
      841 gcctgagtaa taccgatatc tgaagcgcca aaaacgaggc agcccatcaa tagcagcaaa
      901 ggtaaaataa tgcgcaaata aatattttct ttgcggaaaa ccaatgacgc ccctgccgcc
      961 actggtagaa aatacatatg taccgaacgc accacgcccg ggatcggcac atccagcgca
     1021 caaatcaacg caattatggc ggccagccca tgcgccatac atatggcagc gaacctttcc
     1081 cgaccagcat taatcatcgc taccgcatat aaactcaaca acaccaacga catattgcat
     1141 gccatcaata tccactttgc ttgcgataga tagtagagcc cccaagcagt gccgagaatc
     1201 agagcagccc aagcaccaat tgccagcaaa agccttactc gcagtgtatg cggctcttgg
     1261 gcatgctgaa aaaaatcagc cttaaaaagc tttgaaacga accgtagttg cgtggggagt
     1321 tctttgaagt gcggcataat ccatggccaa gattaacttg aatattaccc agcttactct
     1381 tatgactcag gtgcgaaata ggctaaaaag atagcaaata gcgcttacct tgctcattat
     1441 gactgggcag cttcatcaaa acgaacccag gattgttcat cacctggggg catcatgaaa
     1501 aggcacaccg gatacatcta cccttgcggt aaacggcgac cccgctatca taggcattcg
     1561 cccttagacc ggaagttttg cgcccctacc tttcagcagg tttgcctttt tatgatactt
     1621 atcaactcaa caatctttga cggcaatgtc aattaataca cttaatcaaa gattctgcct
     1681 aagcaattta accctgttta tgcagtatgt caaataacag acccgggact actccgggaa
     1741 caaaaaagcc cgtaactcat tgatttacgg gctaaattga ttttttgtac aactacatgg
     1801 tgccgggagc cggaatcgaa ccggcacgcc ttgcggcgct ggattttgag tccagtgcgt
     1861 ctaccagttc cgccatcccg gcatgggata taatgttgga catcaaagac cgctattata
     1921 accataattt tgatttatga gaactctaga tttcgatttt tatctaccac cgcacctgat
     1981 tgcacaacac cccttgccca accgttcaga aagccgcctg ctggaagtca acgcggcaaa
     2041 caataccatt caggataatg atttccgtca catcaccgat cacttcgaaa gtggtgatgt
     2101 actcgtcctc aatgacacca aagtcatcaa agccagacta ttcggccaaa aagccacagg
     2161 cgggcaaatc gaagtgatga tagaccgcgt gctgtctgcg caagaagcct acgcacaaat
     2221 caaatcctca cgttcaccca aggcgggtag tttgttgcac ttatcgggag attaccaggc
     2281 cgaggtattg ggcagacatg cagacatgtt ccatatccga ttgctgggca atgagtcctg
     2341 gtatgacttt ttggaacaat acggccaatt acctttgcca ccatacatag aacaccaggc
     2401 agatgctgca gatgacaccc gctaccagac tgtatttgcc aaacacgctg gtgccgttgc
     2461 tgcgcccact gctggactgc actttgatga tgctctgctg caagccctca tgaacaaagg
     2521 cgtacatgtg cgttacgtga cactgcatgt gggtgcgggg acattccagc ccgtcaaagt
     2581 cgacaatatc agtgagcacc atatgcactc cgaggtctac cacttgccgg aagataccat
     2641 acaaactatt ctggatgccc gggccagcgg caaacgtatt acagctgtcg gcaccacttc
     2701 cctacgcacg ctcgaaagcg cggcgcatgc tggcaaactg tttgcgcatc acggtgagac
     2761 cagtatcttt attacacccg gctttgaatt caagctggtg gaccgcttaa tcacaaactt
     2821 tcacctgcct aaaagcacct tgatgatgct ggtatccgcc tttggggggt atgacttgat
     2881 gcgtcaggcc tatagccatg cgattgaaaa agcttaccgg ttttttagct atggcgatgc
     2941 catgttgatt acacgcgctt catcctcaaa atcttaaact cacacgataa aatagagtct
     3001 atcacttacc caataacact aaatggatct ggagatatgc aattcaacaa tacactgagt
     3061 ggactggcac tcttctgcct ggcgtttggc gcacatgccg acatcctgcc agaggctgaa
     3121 gttggcatca aaatccctct cactaaaaaa ccgactacac gtccaatggg ggtcgcttac
     3181 ttgcccagca accagcatta ctatattgct gatggcggat tagcgcccat gggcagcgag
     3241 tttgaagcac caatttccaa atcggaagtc cacgctttca cgagcaatgg tcaatacgtc
     3301 aactcagcca aacccggcta tgacaatcgc tcgctgtatt acaacccaaa ctcgggcaag
     3361 ctggaaatca ttacctataa catctccagc gcagtaggat tttcacccaa tacgggtatt
     3421 tatagtttgg atgtagatga caaaggcgag ttgctagcga cttcaggcga agttgcccag
     3481 tttaatcctg cctttggcga cgccggcaca atgcccagct acgacccagc cagcaaacac
     3541 tactacgcca aacaggaccg gggcaatatc gtctttgtcg ttgatgccaa gcagcgcgaa
     3601 aaattgcagg aaatcaaact cgattttgcc aaggcgcaag tcgcacatga tgatgtcagt
     3661 gaccatttta tcgcgttcac tggcgtggca ggtaatgaac tggccttact ggatgttgat
     3721 cacaaagcag tattgatttt tgacttgaat ggtcaatatg tgggcaagag tgcgctaccc
     3781 gcaaccatga agctacgttc gcaaaatcac ttcaatggct tgggttatac caacggcatg
     3841 atgttcgtct atcacgagcc agaaggggag tttggcacgt actatgggtt taaagtagtc
     3901 aaataatcaa gaccaaaagc aaaaaaccgc gccatggcgc ggttttt
//
LOCUS       DATMSK010000261         4655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_185059,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000261 DATMSK010000000
VERSION     DATMSK010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185059"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VNZ84_08655"
     CDS             complement(<1..557)
                     /locus_tag="VNZ84_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HWU67067.1"
                     /translation="MSTSLSVKPTSFWQSLSKFDWIYAVLLLAGGIFSYQRYAGFMDV
                     YEVSFLFGAVAVFSYLGWRWSAIARLVLGVAVLSLIGVYSYQGDLANGSQRFLLKFLL
                     ASQSAVMWMNVLFVLAMLTYWLALFQKSAFTAKVASALTWAATLFGFVGLMVRWYESY
                     LIAPDVGHIPISNLYEVFILFCLITA"
     gene            complement(579..2501)
                     /locus_tag="VNZ84_08660"
     CDS             complement(579..2501)
                     /locus_tag="VNZ84_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HWU67068.1"
                     /translation="MRFAVSLLTLLGIASIIGTVLKQNEPYTNYIVKFGQFWFEMFEM
                     LGLLDVYHAAWFLVILLFLVVSTTLCVIRNAPLMIKEWQVYKEHAAEKSLRLFSHQAS
                     LPTQQSPAALQDQLGAFLTSERYRFKIQQQANGDTLIAAKLGTHQRLGYIFTHAAIVV
                     ICFGGLLDGNLPFKVQELLGMKKIETRDISSRKVPAESRLGVNNLSFRANMTLPEGER
                     DNVAFVRVRDGYLVQDLPFTVALKDFRIEHYATGMPKSFESDIEIIDPELSKPIEATI
                     RVNHPYIHKGIAIYQSDFQDGGSQLKFKLWDLSAATSDGFDVDGVILQKGSLGDGVQK
                     MTVEFNEFRQFNILNLSPDGKGKPHNVGPNTTLKLRNASGQAREYVNYMQPLQLDGRA
                     YFVSGMRETVQDEFKYLRIPADDDMEITGFMHLRQILRNDQAIAEIASQVAKQMPGDN
                     APLKAQFEKSLQHLLVTFKQGGYSELSRIIESAVPKDQREQAAQTYLKLLNRAALQGY
                     QISRAQHQLPPAPENEATVAFIQDSLNAMNDLFFYGTPYYFQLKDYTLKQASGFQLTR
                     SPGQNWVYFGSVMLVLGIFAMFYIKERRAWLLIKPAQQEVLLAMSSNRKNMDLDQDFA
                     RMQQQLTDLLQATPSA"
     gene            complement(2714..3343)
                     /locus_tag="VNZ84_08665"
     CDS             complement(2714..3343)
                     /locus_tag="VNZ84_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWU67069.1"
                     /translation="MLKSFKAGLFVLFAMSVQPVFAEEAVSAEAAPAVNGPEKIVQNV
                     CAACHGADGNSVITTNPKLAGQHPEYLLKQLSNFKDGSRANPVMAGMAGALSQEDMEG
                     LAKYFSSQSIKLAKAKSNGKGSLGEKIYRGGIAATQVPACAACHGATGAGLPKQFPRL
                     AGQHSDYTVQQLRTFRTGERANAPMMMAIAAKMSDAEMQAVADYIQGLR"
     gene            3461..4096
                     /gene="yihA"
                     /locus_tag="VNZ84_08670"
     CDS             3461..4096
                     /gene="yihA"
                     /locus_tag="VNZ84_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HWU67070.1"
                     /translation="MPVFQNARFHLSAHHLRDLPPASGIEVAFAGRSNAGKSSALNTL
                     ANHNRLAFVSKQPGRTQLINFFTLGDDRHLVDLPGYGYAKVPEAMRAHWQNVLSRYLS
                     ERTSLAGLVLVMDSRHPLTPLDRQMLDWFCPSGKPVHVLLTKSDKLSRSESTITLNKV
                     RKELHSGWGEKITVQLFSSLKKQGVEDAEKVLGEWFFGQETDAQDEHPVID"
     gene            complement(4139..>4655)
                     /gene="trmB"
                     /locus_tag="VNZ84_08675"
     CDS             complement(4139..>4655)
                     /gene="trmB"
                     /locus_tag="VNZ84_08675"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HWU67071.1"
                     /translation="KILEIGFGMGDATAKIAQTLPDSDFLAVEVHTPGVGSLLKLMQE
                     GDIQNIRIIQHDAVEVLQNMLKDASLDGVHIFFPDPWHKKRHHKRRLIQAEFVKLLCS
                     KLKEGAYIHVATDWQEYAEWVLEVLQAEPLLRNTATDYAEKPAYRPLTKFENRGLKLG
                     HGVWDLVFKRV"
BASE COUNT         1259 a         1293 c         1024 g         1079 t
ORIGIN      
        1 gcagtaatca ggcaaaacag gatgaatact tcataaagat tactgatcgg aatatgacct
       61 acatctggcg caatcaggta ggactcatac cagcgcacca tcaggcctac aaaaccgaac
      121 aaggttgcgg cccaagtcaa tgcagaagcc actttcgcag taaatgctga cttctggaac
      181 aaggccagcc aataagtcag catcgccaat acaaacagca cattcatcca catcaccgca
      241 gactggctgg caagcaagaa cttaagcaga aaacgctgac ttccattcgc cagatcgccc
      301 tgataactat acacaccaat caggctaagg acagccacac ccaacaccag gcgcgcaatg
      361 gccgaccagc gccagccaag gtaactgaac acagcgactg cgccaaacaa aaagctgact
      421 tcatacacat ccataaaacc cgcataccgc tggtaagaga aaatcccacc ggccagcagc
      481 aatacagcgt aaatccagtc aaacttgctt aatgattgcc aaaaagatgt tggctttaca
      541 gataatgatg tgctcattgc attcccctct tcactccgtc aagcggatgg tgttgcttgc
      601 aacagatcgg tcaattgctg ctgcatccgc gcaaagtcct gatccagatc catattcttg
      661 cggttagacg acatggccag caagacttcc tgctgtgctg gtttgatcaa taaccaggcc
      721 cgacgttcct taatataaaa catggcaaaa atacccagca ccaacatgac cgacccaaaa
      781 tatacccagt tctgcccagg tgaccgtgtc aactggaagc cgctggcctg tttcaaagtg
      841 taatctttta actggaaata atatggcgta ccataaaaaa acaaatcatt catggcattc
      901 aggctgtctt gaataaaggc cacggttgcc tcattctctg gcgcaggtgg cagctgatgc
      961 tgggcacgac tgatctggta tccctgcaag gccgcacggt taagcaactt caggtaggtt
     1021 tgcgccgctt gctcacgctg gtctttgggc acagcacttt caataatacg cgacaactca
     1081 ctgtacccgc cctgtttaaa cgtcaccagc aaatgttgca ggcttttttc aaactgtgct
     1141 ttcaatggtg cattatcgcc aggcatttgc ttggccacct ggctggcaat ttcggcaatc
     1201 gcctggtcat tgcgcaagat ctgacgcaaa tgcataaagc ccgtgatttc catgtcatca
     1261 tcagccggga tacgcagata cttgaactca tcctgcaccg tctcccgcat gccagacaca
     1321 aaataggccc ggccatccag ctgcagtggt tgcatataat tcacgtactc acgtgcctgg
     1381 ccactggcat tgcgcaattt caaggtagtg ttaggaccaa cattgtgtgg tttgccttta
     1441 ccatcaggag acaggttaag aatattgaac tgacggaact cgttaaactc taccgtcatt
     1501 ttttgtacgc cgtcccccag ggagcctttt tgcaagatca ccccgtcaac atcaaaccca
     1561 tccgaagtgg cggcggacaa atcccacaat ttaaacttga gttgcgaacc gccatcctga
     1621 aagtcagact ggtaaatggc aatcccttta tgaatataag gatggttaac gcgtatcgtc
     1681 gcctcaatcg gtttactcaa ttcagggtca ataatctcga tatcgctctc aaacgacttt
     1741 ggcatccccg ttgcataatg ctcaatacgg aaatccttga gcgctacggt aaatggcaga
     1801 tcctgcacta aatagccatc acgcacacgc acaaaggcca cattgtcccg ctcaccttcg
     1861 ggcaaggtca tattggcgcg gaaggataga ttattgacgc ccaagcggct ttcggcaggc
     1921 actttgcggg aagaaatatc gcgcgtttca atcttcttca ttcctaacag ctcttgcacc
     1981 ttgaacggca aattaccatc cagcaaacca ccaaaacaga tcacgacgat ggctgcatgg
     2041 gtaaagatat agcccagacg ttgatgtgta cccagctttg cggcgatgag cgtatcgcca
     2101 ttagcctgct gctggatttt aaaacggtac cgttctgaag ttaaaaatgc ccccaactgg
     2161 tcttgtaacg cagccggaga ttgctgggta ggcagactcg cctgatgact gaacaagcgc
     2221 aatgattttt cggcggcatg ctctttatag acttgccact cctttatcat cagcggcgca
     2281 ttgcggatca cacacaaggt agtcgacacc accaggaaca agaggatcac caggaaccag
     2341 gccgcatgat acacatccag caagcccagc atctcaaaca tctcaaacca gaactggcca
     2401 aacttgacga tgtaattggt ataaggctcg ttttgcttga gcaccgtccc aataatcgag
     2461 gcaatgccca acagcgttaa caggctcacg gcaaagcgca tggagctaag taactcataa
     2521 acacgctgcg acaaggaaac gggtgttttc aaaacgttct cttttaaatt gactgattat
     2581 gattgcgccc aagggcggtc agacaagcct ctaacgcaag gcgcgcacat gtggacgaca
     2641 tctacagtaa aaggttgcat gggcacacag taaaaaaagg tggccgcagc cacctttttt
     2701 gccgtcatgc aaattaacgc aggccttgaa tatagtcagc caccgcttgc atctcggcgt
     2761 cgctcatttt cgcggcaatt gccatcatca tcggcgcatt ggcacgctca cccgtgcgga
     2821 acgttctcaa ttgctgcacc gtgtaatcgc tatgctgtcc agccaggcga gggaattgtt
     2881 ttggcaaccc agcacccgtt gcaccatgac aggccgcaca ggcaggcacc tgggtagctg
     2941 caatgccacc acggtaaatc ttctcaccta aagaaccttt accattgctt ttagccttgg
     3001 ccagcttgat actctggctg gagaaatact tggccagacc ttccatatcc tcctggctca
     3061 gcgcacccgc catgccagcc atcactgggt tagcacggga gccatctttg aagttgctca
     3121 actgcttgag caaatactca ggatgctggc ctgccagttt cgggttggtc gtgataacac
     3181 tgttaccatc tgcgccatga caagccgcac agacattttg tacaatcttt tccgggccat
     3241 tcacggctgg cgcggcttcc gctgagacag cttcctcagc aaacactggc tgtacgctca
     3301 tggcaaacag cacaaacaat cctgctttaa aactcttcaa cataattcaa tgccccaaca
     3361 tttttattgg gcgtcatttt atcaaacaaa ccctgttcgt gatagcatgc acagctgaat
     3421 agtccagaaa acattgaggc aactcaattt caatttcatc atgccagttt ttcaaaacgc
     3481 ccgattccat ctctcggcac atcatctgcg cgacctacca cctgccagcg gcatcgaggt
     3541 cgcgtttgcc ggacgctcga atgcaggcaa atccagtgca cttaacacgt tagccaacca
     3601 caaccgactg gcattcgtga gtaaacaacc aggccgcaca cagctgatca attttttcac
     3661 cttaggcgat gaccgccacc tggtcgattt accaggttat ggttacgcca aggttcccga
     3721 agccatgcgt gcgcattggc agaatgtact ttcacgctac ctcagtgagc ggaccagcct
     3781 ggccgggctt gtgctggtca tggatagcag gcacccactc actccgctgg accggcaaat
     3841 gctggactgg ttttgccctt ccggcaaacc ggttcatgta ctcctcacca aaagcgacaa
     3901 attatcgcgt agtgaatcta ccatcacact taacaaagtc cgcaaggaac tgcattctgg
     3961 ctggggtgaa aaaattacgg tgcagctgtt ttccagcctg aaaaaacaag gcgtcgaaga
     4021 tgctgaaaaa gtgctgggag aatggttctt tggtcaagag accgatgctc aagacgaaca
     4081 tcctgtcatt gattaaaaaa agcagaccga agtctgcttt ttttctaggc gccatccatc
     4141 acacgcgctt aaataccaaa tcccataccc cgtgtcccaa tttcaggccg cggttctcaa
     4201 acttagttaa tggccggtaa gcgggcttct cagcatagtc cgtcgccgta tttcttaaca
     4261 aaggttctgc ctgcaatact tcgagcaccc actcggcata ctcctgccag tcagtcgcca
     4321 catgaatgta agcaccctcc ttcaatttcg agcacagcag tttaacaaac tcagcctgaa
     4381 tcaggcgacg cttgtgatgc cgttttttat gccaaggatc ggggaagaaa atatgtacac
     4441 catccagcga ggcatccttt agcatattct gcagaacctc tacagcatca tgctggatga
     4501 tgcggatatt ctggatgtca ccctcttgca tcaacttaag caaactgcca acacccggcg
     4561 tatgcacctc tacagccaga aagtcactgt ctggcaaggt ctgggcaatt ttggctgtag
     4621 catcacccat gccaaagccg atctcaagga ttttt
//
LOCUS       DATMSK010000262         6894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_421099,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000262 DATMSK010000000
VERSION     DATMSK010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421099"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1509
                     /locus_tag="VNZ84_08680"
     CDS             7..1509
                     /locus_tag="VNZ84_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU67072.1"
                     /translation="MFTVLFGSPLIPSKATFALGVLQSLGSLLLIVVSLRVVYDTWRW
                     SRMTRLLISAILLWFFVQVYWLLSSHASYSHIFSTDNMSWDTSHRQIATDFILSLLVM
                     LGYVLCHFRFNRASQTILSIAILMPATGLLAQAYHIPDAYGQLSLLASVAGVLCAMSL
                     IAKQVSQTPMSYLLLLDDTGQHLRWIVAALFLCAIALGGLGVLFGHDPQLTPLLITLA
                     LNAIVIIMTLNYYRKGVMHDQVLPPDELAFASELEAAIAHQEFYLDYQPQIDFMTGKL
                     VGVEALIRWRHPQLGVVPPAKFIGVAELTGLIVPMGAWVLKAACQQVAAWKTGALSKV
                     KVSVNVSPLQLRTPGFNDFVLQVLRETGLSPERLVIELTESAFVHSDCTGIESLQALK
                     QLGVKLAIDDFGTGYSCLAYLRDIPGDYLKVDRSFVNDVPGKERSEAVTSAIVVLGKN
                     LHYQIVAEGVETQAQADYLKSLGCDLAQGYLYAKPMSEQALQHWVDERHS"
     gene            complement(1521..2852)
                     /locus_tag="VNZ84_08685"
     CDS             complement(1521..2852)
                     /locus_tag="VNZ84_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWU67073.1"
                     /translation="MKFDPRNNKFTAIFTREMLEATLPSILLISLALFFAYKFIDPAP
                     PRHIVIATSNDDLNYNAFASVYGVYLQKEGITLEVRKTAGDNDNLRLLKDPDSGVDIA
                     FIQDGVAHSEGAGSLLSLGSLYYQPVWIFCRCKAETTHLSALKGKRIAIGYAGNGTHT
                     VVSTLLAESGINASNTKLVAIGGEEASAALHEGKVDAALFVDVANSYIIREMVADKRL
                     HLLSLDTAEAFSRKFSFMHHLTLPEGALDLARNIPHHDVQLVAPTATLVVKESMHPAL
                     VYLMMKVISEVHGGPSMFNSKGEFPAAKDTDFPISTQALNFYKSGLPIIDKYLPFWAA
                     TFVNRTLIVILPLLALLIPLTKIIPMVYVWLVKRKLFRYYGELRFLDAQLQDIKTAED
                     VQQCLDKLNDIEAKVVNVKLPVPFSQYAYELRAHIELVRSKLYHHKQAQQP"
     gene            complement(3001..4902)
                     /locus_tag="VNZ84_08690"
     CDS             complement(3001..4902)
                     /locus_tag="VNZ84_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HWU67074.1"
                     /translation="MTTSNSIDLASFEPEAILASLPGEHPKMTAALGVGRELENTGVL
                     DTATLTQLVNDLYAEGFPATAPFAAADFAQSLASNHTPQPSVPATDSLTTKLGHAGGN
                     SLGATSPSYVQAQGQPVTQEAQAQQLTYAPGIEALGSDPLHNHPAHLNVLQPDAAYLA
                     PTAFAGLGIEIPYQQELDRWLGTGASQSPSSAAPTSSASKSSTGSSQSFYFLQAEPAV
                     IGSLPASAHPAFDVHAVRRDFPILNERVNGRQLVWFDNAATTHKPQVVIDRISYFYQH
                     ENSNIHRAAHALAARATDAYEDARETVRRFINAPSVNNVIFVRGTTEAINLVAKTWGK
                     KHLGAGDEIVVSNLEHHANIVPWQQLCQETGAVLKVIPVDDSGQILLEEYQKLLTART
                     KLVSFTQVSNALGTVTPAQEMIAMAHRAGAKVLLDGAQSVSHMRTDVQTLDADFFVFS
                     GHKIFGPTGIGALYGKVDVLQDMPAWQGGGNMIQDVTFEKTVYHAAPAKFEAGTGNIA
                     DAVGLGAALKYVERLGIENIAAYEHALLEYATAAMCQIPNLRLIGTAKHKASVLSFAL
                     KGYQSEEVGAALNEEGIAVRSGHHCAQPILRRFGVETTVRPSLAFYNTYEEIDRMIAV
                     LQRLASYHA"
     gene            complement(4889..5803)
                     /locus_tag="VNZ84_08695"
     CDS             complement(4889..5803)
                     /locus_tag="VNZ84_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HWU67075.1"
                     /translation="MSEQPTQSALGDVAARTLANATKSVPMLSTISPRWLVHLLNWVP
                     VEAGIYRVNKAKDPNAITVDCSNKDEKELPATFVDYEEWGREYVLNAVNTVVDVHTRV
                     SDLYSSPHNQIKEQLRLTIEVVKERQESELINNKEYGLLNNVAKSQTIKTRNGFPTPD
                     DLDELLTKVWKEPAFFLAHPKAIAAFGRECTRRGVPPPTVSLFGSQFLTWRGIPLIPS
                     DKLPIVNGKSKILLLRVGESKQGVIGLYQPNLPGQQTMGLSVRFMGINHKAIASYLVS
                     LYCSLAVLTEDAIAVLEDVGVDHYYDYK"
     gene            complement(5862..6764)
                     /gene="epsC"
                     /locus_tag="VNZ84_08700"
     CDS             complement(5862..6764)
                     /gene="epsC"
                     /locus_tag="VNZ84_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HWU67076.1"
                     /translation="MEEIAIEHIVQQLASIRESALQQRQRLSQPPKLPARKALGKCVE
                     WLAASLFPNRLGDSDIQAQAINFYVGTTLDKALRELQQQVRLELAFKAPGTPASQFNT
                     QAHAITHAFAEQLPHIRIQLDTDLEAAYQGDPAADSVDEILVSYPGILALIHYRIAHV
                     LHGLGLTIIARLITEIAHSQTGIDIHPGARIGDYFFIDHGTGVVIGETAIIGNHVRLY
                     QAVTLGAKNFPKDANGHLVKKFERHPILEDYVVVYAGATILGRITIGKHSIIGGNVWI
                     TRSVPVNSHVTQAHSDAKPIPAAA"
BASE COUNT         1551 a         1723 c         1870 g         1750 t
ORIGIN      
        1 gccagtattt ttaccgtgct ttttggttct cccctgatcc cgtcaaaagc cacttttgca
       61 ttgggcgtgt tacaaagtct tggttccctg ctcttgatcg tcgtcagttt gcgtgtggtt
      121 tatgacacct ggcgttggtc acgcatgaca agactgctga tcagtgccat tttgctatgg
      181 ttttttgtcc aggtgtattg gctattaagc agtcatgctt cttacagcca catattttca
      241 accgacaata tgtcctggga taccagccat agacagattg ctactgactt tatcctgtca
      301 ttgttggtga tgctcggcta tgtgctttgt cacttccgct ttaatcgagc cagccaaact
      361 atcctctcta tcgcgatact gatgccagcg acaggtctgt tggcgcaggc gtaccatatc
      421 ccggatgcgt atgggcagct atctttgtta gcctctgttg ccggggtgtt gtgtgccatg
      481 tccctgattg ccaaacaggt ctcacaaacg cccatgagtt atctattgct actggatgat
      541 accggccagc atttacgctg gattgttgca gcgctgtttt tgtgtgcgat tgcactaggt
      601 ggcctgggtg tgctgtttgg gcatgacccc cagcttacgc cgttactgat caccctcgca
      661 ttgaacgcca tcgtcattat catgaccttg aattattacc gtaaaggtgt catgcatgac
      721 caggtgctgc cacctgacga actggctttt gccagtgagc tggaagcggc gattgcacac
      781 caggagtttt atctggatta tcagccgcaa atcgatttca tgactggcaa actggtcggc
      841 gtagaagcgc tgatccgctg gcggcatccg caacttggcg tggtgcctcc ggccaagttt
      901 attggtgtcg ctgaattgac aggcctgatt gtgcccatgg gagcctgggt gctaaaagca
      961 gcttgccagc aagtcgccgc atggaaaaca ggggctttga gcaaagtcaa agtgtcggtc
     1021 aatgtgtcgc ctttgcaact gcggacacca ggctttaatg attttgtatt gcaggtattg
     1081 cgtgaaaccg gcttgtctcc ggagcgactg gtgattgaac tgaccgagag tgcgtttgta
     1141 cattcagact gtacaggaat tgaaagttta caggccctca aacagcttgg ggtcaaactg
     1201 gcaattgatg atttcgggac cggttactca tgcctggctt acttacgcga tatccctggt
     1261 gattatctga aagtagaccg ctcttttgtg aatgatgtgc cgggcaaaga gcgctcggaa
     1321 gccgtgacca gtgcgatcgt ggtactaggt aaaaacctgc attaccagat tgtggcagag
     1381 ggcgtggaga cacaggcaca agcagattat ctcaaaagcc tgggatgtga tctcgcacaa
     1441 ggatatttat acgccaagcc catgtctgaa caagcgttac aacactgggt tgatgagagg
     1501 cactcttaac cagtgcctgt tcaaggctgt tgagcttgct tgtggtgata cagtttggac
     1561 ctgaccagct cgatatgcgc gcgtagttcg taagcatatt gtgaaaatgg taccggcaat
     1621 ttgacgttca ccactttggc ttcaatgtca ttgagtttat ccaggcattg ctgtacatcc
     1681 tcagcggttt taatgtcctg caactgcgca tccaggaaac gtaactcgcc gtaataacgg
     1741 aacagcttgc gttttaccag ccacacatac accatcggga taatcttggt cagcgggatc
     1801 agcaaagcca gcaatggcag gatgacgatc agggtacggt tcacaaatgt agcagcccag
     1861 aatggcaggt acttatcaat aatcggcagg ccggacttat aaaaattgag cgcttgcgta
     1921 ctgatgggaa agtctgtgtc tttagcggcg ggaaactcgc ccttgctatt aaacatgctg
     1981 gggccgccgt gtacttcgga aatgactttc atcatcaggt acaccagcgc cgggtgcatg
     2041 ctttctttca ctaccagggt cgccgtgggg gccaccaact gcacatcatg atggggaata
     2101 ttgcgcgcca ggtctagcgc accttctggc agtgtcaggt gatgcataaa ggaaaacttg
     2161 cgtgaaaatg cttctgcggt gtccaggctg agtaagtgta agcgtttgtc tgccaccatc
     2221 tcgcggataa tgtaagaatt ggcgacatct acaaacaggg cggcatccac tttgccttca
     2281 tgcagtgcgg cggaggcttc ttcaccgccg atcgccacca gtttggtgtt gctggcatta
     2341 atgccacttt ccgccagcag ggtactcacg acggtatggg tgccgttccc cgcataacca
     2401 atcgcgatac gcttgccctt gagcgcggac aaatgtgtgg tctctgcctt gcatcggcaa
     2461 aaaatccata ccggctggta atacaggctg cccagcgaca gtaatgaacc tgccccctcg
     2521 ctgtgggcga caccatcttg gataaatgcg atatcgacgc ctgagtcagg gtctttcagc
     2581 aagcgcaggt tgtcattgtc accagccgtt ttacggacct ccagcgtgat cccttctttt
     2641 tgcaagtaca ccccatacac agatgcaaag gcattgtagt taaggtcgtc attgctggtg
     2701 gcaatcacaa tatggcgggg tggggcaggg tctatgaatt tgtaggcaaa aaacagggcc
     2761 aggctaatca aaaggataga gggtaaggtc gcttccagca tttccctggt aaatatagcg
     2821 gtgaacttgt tgttgcgcgg atcaaatttc atcggtctgc tttatcgttt gtctgtgcct
     2881 gagtcaggga ttttacaggg cttagaccga atgccacaac agctggttgt gcctgggcaa
     2941 gaaaagaccg tgtatgcatc tcaccagaaa tgcatacacg gctacaggtt tacaattggc
     3001 ttaagcgtga taagacgcca ggcgttgcaa tactgcaatc atgcggtcga tttcttcata
     3061 ggtgttgtaa aacgccagtg aagggcgcac cgtggtttcg acaccaaacc ggcgcaaaat
     3121 gggttgcgcg caatgatggc cggaacgcac tgcaatacct tcttcattga gcgccgcgcc
     3181 gacctcttca ctctggtaac ccttgagggc aaacgacaac acgctggctt tgtgtttggc
     3241 ggtgccgatc aggcgcaggt tggggatctg gcacatggct gcggtcgcat attcaagcaa
     3301 ggcgtgctcg taagccgcga tgttctcaat gcccagacgc tccacatatt tgagtgctgc
     3361 tcctaaacct actgcatctg caatattacc agtgcccgct tcaaacttgg ctggagcagc
     3421 gtgatagacc gttttttcaa aggtaacatc ctgaatcata ttgccgccgc cttgccaggc
     3481 aggcatgtct tgcaacacat cgactttgcc atacagggca ccaataccag tcgggccaaa
     3541 aatcttatgt ccggaaaaca caaaaaagtc agcatccagc gtttgcacat ccgtgcgcat
     3601 gtgtgaaacc gattgcgcgc catcgagcaa tacttttgcc ccggcgcgat gtgccatggc
     3661 aatcatttcc tgggctggtg tgacggtgcc cagcgcatta gagacttgtg taaacgacac
     3721 cagcttggtt ctggccgtga gtagtttctg gtactcctcc agcagaatct ggccgctatc
     3781 atccaccgga atcactttta gtaccgctcc tgtttcctgg cacagttgct gccacggtac
     3841 gatgttggcg tgatgttcga ggttagaaac cacgatctcg tcaccagcgc ccaaatgctt
     3901 cttgccccag gttttggcga ccaggttaat cgcctctgtt gtcccgcgca caaaaatcac
     3961 attattcact gacggtgcat tgataaagcg gcgcacggtt tcacgcgcat cttcataagc
     4021 atccgtcgcg cgtgccgcca gtgcatgcgc cgcacggtga atattggagt tttcgtgttg
     4081 gtagaaataa ctgatacggt ctatcaccac ctgcggtttg tgtgtcgttg ccgcattatc
     4141 aaaccacacc agctgacggc cattgacgcg ctcgttcaga attgggaagt cgcggcgcac
     4201 ggcatgcaca tcaaacgcag gatgcgcaga ggcgggcagg ctaccgatga cggctggttc
     4261 agcttgcaaa aaataaaagg actgcgagct gcctgtggat gatttgctgg ctgatgatgt
     4321 tggtgcggct gatgacggag actggcttgc gcccgtcccc aaccagcgat ccagttcctg
     4381 ctgataggga atctcaatgc ccaacccagc aaatgctgtc ggtgctaggt aagcggcgtc
     4441 aggctgcaac acattcaggt gtgccggatg attatgcaac ggatcgctac cgagggcttc
     4501 gatgcctggt gcataggtga gctgttgcgc ttgtgcttct tgcgtgacag gttggccttg
     4561 cgcctgcaca tagcttggcg aggtggcacc taacgagttg ccgccagcat gccctagttt
     4621 cgtggtcaac gaatctgtgg caggcacaga gggctgtggt gtatggttgc tggccagcga
     4681 ctgcgcaaaa tctgctgcgg caaacggcgc cgtcgccgga aagccttcag cgtaaagatc
     4741 atttaccagt tgcgtcaggg tagcggtatc cagcaccccc gtgttttcca gttcgcgacc
     4801 aacgcctagt gcagctgtca tctttgggtg ctcgcctggc agtgaggcca aaatggcctc
     4861 aggctcaaag ctggctaagt cgatagaatt acttgtagtc atagtaatgg tcaactccga
     4921 catcttccag tacggcaatc gcatcctcgg tcagcacggc cagtgagcaa tacagggaga
     4981 ccaggtagga ggcaatcgct ttatggttga tacccataaa acgtacggat aagcccatgg
     5041 tttgttgacc aggcaagtta ggttgataca agccaatcac accttgtttg ctttcgccta
     5101 cgcgcagcaa caggattttg gatttgccgt tgacgatagg cagtttgtcg ctaggaatca
     5161 gtggaatgcc gcgccaggtc aggaactgtg aaccaaacag tgacacggtg ggtggtggca
     5221 cgccgcgacg ggtgcattca cggccaaagg cagcaatcgc tttcgggtgt gccaggaaga
     5281 aggcaggttc tttccagact ttggtcagca attcatccag gtcgtccggg gttgggaagc
     5341 cgttacgggt tttgatggtt tgtgacttgg ccacattgtt gagcaggcca tattccttgt
     5401 tgttgatcag ttcgctttcc tggcgttctt tcacgacttc aatggtgagg cgcaactgct
     5461 ctttgatctg gttgtgcggg ctgctgtaga ggtcagagac tcgggtatgc acatcaacca
     5521 cggtgttgac cgcattcagc acatattcac ggccccattc ttcatagtcc acaaaggtgg
     5581 ctggcagctc tttttcgtcc ttgttagagc aatccacggt aatcgcattg gggtctttcg
     5641 ccttgttaac acggtaaata ccggcttcta ccggcaccca gttcagcaaa tgcaccagcc
     5701 agcgtgggct gatggtgctg agcatgggca ccgatttggt ggcattggca agcgttcgtg
     5761 cggcgacatc gcctaaggcg ctctgggttg gttgttctga catacgatga ttctccttat
     5821 gaaattagct aaacattagc ggctaaaggt tgtggtaaac atcaggcggc agccgggatc
     5881 ggtttggcat ccgaatgcgc ttgtgtgaca tggctattga caggcacgct gcgggtgatc
     5941 cagacgttgc cgccaataat ggaatgtttg cctatggtga tgcggccaag gatggttgcc
     6001 ccggcgtaga ccaccacgta gtcttctaaa atgggatggc gttcaaactt tttgaccaga
     6061 tggccattgg catctttagg aaagttttta gcacccaagg tcacggcctg gtagaggcgg
     6121 acgtggtttc cgatgatggc ggtttcacca atgaccacgc cagtaccatg gtcgataaaa
     6181 aagtaatcgc caatgcgtgc ccctgggtga atatcaatcc cggtttgcga gtgggcaatt
     6241 tcagtgatca agcgcgcaat aatggtcagg cccagaccat gtaatacgtg ggcaatgcgg
     6301 taatgaatca aggccaaaat gccaggataa ctgaccagga tttcatctac gctatctgcg
     6361 gccggatcgc cctgataagc ggcttccagg tcagtatcca gttgtatgcg gatatgcggc
     6421 agttgctcgg caaaggcatg cgtaatggca tgcgcttggg tgttaaattg actggctggt
     6481 gtgcctgggg ctttaaaagc caactccagg cgaacttgtt gctgtaattc tcgcaaggct
     6541 ttatccagcg tcgtgcctac ataaaagtta atggcctgcg cctgaatgtc agaatcgccc
     6601 aagcgattag gaaacagcga tgccgccaac cattccacgc atttccccag tgccttgcgc
     6661 gcaggcagct tgggcggttg agacaagcgt tgtcgctgct gcaatgcgct ttcgcgtatg
     6721 ctggccagtt gttgcacgat gtgctcaatc gcgatttctt ccatcggcta ttccatcctg
     6781 tctttaaagc attcactgct ggctacagac gaaaaaaaag ccagtcgccc acttagggtg
     6841 actggcttcc aggtgtctgg tcagcattaa tctttttaaa ctataaaata atcc
//
LOCUS       DATMSK010000263         8128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_115920,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000263 DATMSK010000000
VERSION     DATMSK010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115920"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VNZ84_08705"
     CDS             complement(<1..983)
                     /locus_tag="VNZ84_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU67077.1"
                     /translation="MTDNAVRWSHAGETYQADWLSSIQPNPPKWFEVIGDQTNADTAY
                     RLASEGCGMIYQGDFQNARQLLQALARRIDKKPLKGAPTLLETFHLHRARQIQRANIL
                     NKIILHIENGRSAMKRAPEMSEALGNALDGGVPASLLIPLQMLQGMVGANEWRKKGVM
                     IPVLSQNIHPYYGVYSPVRGEYLDLIAQAPLQAKTAMDIGTGTGVIAALLAKRGVPSI
                     IATDNQPRALACAQDNLQRLGFTHKVKVVEADLFPADSQAELIVCNPPWLPAKANAPI
                     EHAVYDPDSRMLKAFLNGVKAHLKPNGEAWLIMSDLAEQIGLRKPGELAGW"
     gene            complement(980..1684)
                     /locus_tag="VNZ84_08710"
     CDS             complement(980..1684)
                     /locus_tag="VNZ84_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HWU67078.1"
                     /translation="MRKQYRYYDLIMAAFAVVLVCSNIIGPGKITEIHVPLLGALTFG
                     AGIMFFPISFIFGDILTEVYGYAASRRVIWTGFVCLAFASFMAWVIVALPPAPEWHDQ
                     AAYQSVFGSTARIAIASLVSFVAGEFVNSFVLAKMKILTQGRWLWSRTIGSTIFGEAV
                     DTILFFPLAFYGSGEFPDAMMPQIVLAQFTAKVLVEVAFTPITYKIVAFLKRAENEDY
                     YDTKTDFNPFTLETNR"
     gene            complement(1681..2478)
                     /locus_tag="VNZ84_08715"
     CDS             complement(1681..2478)
                     /locus_tag="VNZ84_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU67079.1"
                     /translation="MKTFSLLILGFWLLVVMLGHVVGLEPNRTDLNAILQWPSQQHWL
                     GTDDLGRDILARVIHGVEVSFMVTLVVTAVTMTVGLSIGLVAGYKGGKLDAVLMQITN
                     IFLAFPGMLLAIAFAAVLGAGINNLILALCLTGWVTYARLSRGQSLALRQRLHVLAAE
                     SLGAGSMRVMLLHILPLLISILLVEAAYSMASVMIAEASLSFLGLGIQAPNASWGAML
                     RDAVRFMLVAPHYVLTVGACMMSLILAINLGGDVLRDKLDVRSERQV"
     gene            complement(2475..3386)
                     /gene="nikB"
                     /locus_tag="VNZ84_08720"
     CDS             complement(2475..3386)
                     /gene="nikB"
                     /locus_tag="VNZ84_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HWU67080.1"
                     /translation="MIKRFATLLPVVFGVLCLTFALLHLVPGDPVDVMLGESASPADR
                     VAIKAQLGLDQPLPVQFGQYLSHLLRGDFGQSIHTQTPISQLLWQAYPATLLLALVAL
                     AIGLVIGIPLGIWSALKAGHWQDVMITMLSIRLAAMPAFWLGPVLMLVFAVWLGWLPV
                     SGMGSGAAILLPALTLGLGLSAILTRMTRTSLLEVLNEDYIRTARAKGLSERQVLLKH
                     ALRAALLPLVTIVGLQMGSLLAGAVITETIFSWNGIGRLLVDSIEKRDYPVTQACVLV
                     VAFSYVLINQATDVIYRWLDPRTRGTQ"
     gene            complement(3379..3867)
                     /locus_tag="VNZ84_08725"
     CDS             complement(3379..3867)
                     /locus_tag="VNZ84_08725"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWU67081.1"
                     /translation="MHIEISIPAQTLTLFDDNGGQVTRYAVSTAANGAGCVKNSGCTP
                     LGAHIIRAKIGAGAAENTVFVGRRPTGETFTPDLKVQFPERDWILTRILWLSGTEPGK
                     NRLGNVDTMQRYIYIHGTPDETVLGVPGSHGCVRMRNADLIELFDLVPVGTTVNICEA
                     ND"
     gene            4004..4693
                     /locus_tag="VNZ84_08730"
     CDS             4004..4693
                     /locus_tag="VNZ84_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HWU67082.1"
                     /translation="MAHHELHFLQRSGWLRAAVLGANDGIISTASLLMGVAAAGASHQ
                     AMLMTGIAGLVAGALSMAAGEYVSVSSQTDIEQADLAREAEELKENPESELRELTGIY
                     IQRGLSPELARQVAQALSQRDSLAAHARDELGLHDNNQAQPLQAALTSALAFSSGALL
                     PIVTAWLAPHSQAQTWLTISTLPFLGLLGMVSARTGGANVLKSVSRVVFWGALALAAT
                     ALIGRWFGVNP"
     gene            complement(4717..6186)
                     /locus_tag="VNZ84_08735"
     CDS             complement(4717..6186)
                     /locus_tag="VNZ84_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU67083.1"
                     /translation="MRKHNVVALALHRWLPGLLLTVALTGCQSADEPHPLVFAIAQAP
                     VNLDPRYATDAASERINRLIYQRLVEFDASSREVPGLATWQMQDAQHYRFQLHQSARF
                     HDGQPLTALDVKATYESLLQLKDSPHTAEFRHIQNITTPDAGTIDFTLSHPDPHFAAR
                     MMVGILPARLIAAGHDFGHAPVGSGPFKMDRWQSALQLTRLTDGLKVRFEEVKDPNVR
                     VLKLKRGEADLIQGDLPPELVKYLQKQSHVTVSTGIGANYSYLGLNVQADFLKDVRVR
                     RAIAHAIDTRAIIDKVMVSHTREANAILPPEHYAGNPELQPYRYQPALARQLLQSAGV
                     QLPLTLIYKTSTDPQRVRLATILQAQMQEAGIDLQIKSLDWGTFFADVQKGNFQMCGL
                     TWVGIKTPEIYSKVFASNSLPPQGFNRGRYQDPVLDQLLEKENWPAVTTRLHSQLPVI
                     PLWYEGQFTAYQNTIASYQPMPDGNWDGLTQVVFKPAAD"
     gene            complement(6173..7546)
                     /gene="rlmD"
                     /locus_tag="VNZ84_08740"
     CDS             complement(6173..7546)
                     /gene="rlmD"
                     /locus_tag="VNZ84_08740"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HWU67084.1"
                     /translation="MRNNRQRSSNLSSAAKAAQIPRTAIIESLDQEGRGVTHVDGKVI
                     FIEGALPFEEVRFVSHHVKSTYELATTVEVVKASSQRVTPGCAYFGACGGCAMQHLEF
                     SAQVAAKQRMLEDDLWHIGRVRPEKILPPIQGTPWHYRHKARLRVRYVEKKGGVLVGF
                     NEKGSSFVTFMDSCEVLTQPVSDLIVPLKQLIGQLSIKQSLPQIELAVGEDATVLVFR
                     ILEALTEADIALMQTFEETYGAQVWTQTKGPDTVKPLFEHGDKLHYSLPEYNLEYPFS
                     PTEFTQVNPAINRVMIRRAMQLLEPKAGERIADFFCGLGNFTLPIARSGASVYGVEGS
                     QALVKRAIENAGLNGVENVEFAESDLFDMTAQSLQSLGAFDKWLVDPPRDGAIELIKA
                     LPDAGDPASGHAPSRIVYVSCNPSTLSRDAAILTQVKGYHLRAAGVMNMFPHTAHVES
                     IAIFEKA"
     gene            7614..8102
                     /locus_tag="VNZ84_08745"
     CDS             7614..8102
                     /locus_tag="VNZ84_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67085.1"
                     /translation="MIKQWGLATTLAIALSSPNFSFADELAVQQDSHGTDYVTGGIGS
                     EEVDAMEVFKKQFNLYFLFSEGKVGRVIDDINISITDSKNQTVFALEHAAPRLLLNLP
                     SGKYQATASYLGSEQRYRFTHDAKKHQRIILNWKNKIDEDTLEAAPEDPAEANAPTPE
                     TP"
BASE COUNT         2088 a         2253 c         1980 g         1807 t
ORIGIN      
        1 atccaaccgg ccagctcgcc aggtttacgc aatccaatct gctcagccag atcagacata
       61 atgagccaag cttcgccatt aggtttcagg tgtgctttca caccgtttaa aaaggcttta
      121 agcatgcggc tgtcaggatc atacacggca tgctcgatcg gtgcattggc tttggcaggt
      181 aaccaaggcg ggttacaaac gatgagctct gcttggctat ctgccgggaa taaatcagct
      241 tcaaccacct tcaccttgtg agtgaaaccc aggcgttgca agttatcttg tgcacatgcg
      301 agtgcacgcg gctggttatc cgtcgcgatg atactaggca caccccgctt cgccagcaag
      361 gcagcaatta cacccgttcc cgtgccaata tccatggctg tttttgcctg taatggggcc
      421 tgtgcaatca agtccaggta ttcacctcgc acaggagaat acacaccgta gtaaggatga
      481 atgttttgac taagcactgg gatcatcact ccctttttgc gccactcgtt tgcacccacc
      541 attccttgta gcatttgcag cgggatcagc aatgacgcag gcacaccgcc atccaaagca
      601 ttgcccagtg cttcactcat ttcaggcgca cgcttcatgg cactgcggcc gttttcaata
      661 tgcaggatga ttttattaag gatattggcg cgttggattt gccgtgcacg gtgcaggtgg
      721 aaggtttcga gcaaagttgg tgcgccctta agtggctttt tgtctatccg gcgcgccagc
      781 gcctgtagca actggcgcgc attctggaag tcgccttggt aaatcatgcc acaaccttca
      841 gacgccaagc ggtaagccgt atcggcatta gtctggtcac caatgacctc gaaccacttg
      901 ggtggattgg gttgtatgct actcagccaa tcggcctggt aggtttcgcc agcgtggctc
      961 cagcgaacgg cattatcggt catcgattgg tttccagtgt aaacgggtta aagtctgttt
     1021 ttgtatcata gtaatcttca ttttcggcgc gcttgagaaa ggcgacaatt ttgtaggtga
     1081 tgggcgtgaa agccacttct accagcactt tggcggtaaa ttgtgccagt acgatctgcg
     1141 gcatcatggc atcggggaat tcgcctgaac cataaaaagc cagcggaaaa aacaggatgg
     1201 tatcgaccgc ttcgccaaaa atcgtggagc ctatggtgcg gctccataac caacggcctt
     1261 gcgtcagtat cttcatcttg gccagcacaa acgaattgac aaactcgcct gccacaaatg
     1321 agaccaggga ggcaattgca atacgtgccg tagagccgaa cacagattga taggctgcct
     1381 ggtcatgcca ctccggcgcg ggtggtaaag cgacaatcac ccaagccata aagctggcaa
     1441 atgccagaca aacaaagcct gtccagatga cacgacgcga agcagcgtac ccgtagacct
     1501 cagtcaggat gtcaccaaaa ataaaactga tggggaaaaa catgatgcct gcgccaaatg
     1561 tcaacgcgcc cagtaatggc acatgaatct cggtgatttt gccgggcccg ataatgtttg
     1621 agcacaccaa caccacggca aacgcggcca taatcaggtc atagtaacgg tattgttttc
     1681 tcatacctgc ctttcgctac gcacatccaa cttgtcacgt aacacatctc cccccaggtt
     1741 gatggcgaga atcaaactca tcatgcatgc acccacagtg agcacataat gcggtgccac
     1801 cagcataaag cgcaccgcat cgcgcaacat ggcgccccac gaagcatttg gcgcttgtat
     1861 gcccaggcca agaaacgaaa gcgaggcttc agcaatcatc acactcgcca tgctgtacgc
     1921 agcctcgacc agcaggatgg aaatcagtaa aggcaggata tgcaacaaca tgacccgcat
     1981 actgccagcc cctagcgatt ctgcagctag cacatgcagg cgctggcgta aggccaatga
     2041 ctggccacgg gacaatctgg cataggtcac ccagccggtg aggcacaatg caagaatcag
     2101 gttattaatc ccggcgccca gcaccgccgc aaaagcaatg gccaacagca tcccagggaa
     2161 cgccaggaaa atattagtaa tttgcattag aacagcatcc agtttgccac ctttataacc
     2221 tgccaccaat ccgatactta gtccaaccgt catggtcacc gccgtcacca ccagcgtcac
     2281 cataaaagag acttctaccc catgaatgac ccgcgccagg atatcgcgac ccaaatcatc
     2341 tgtccctaac cagtgctgct ggctaggcca ttgcagaatg gcattgaggt cagttcgatt
     2401 gggttccaac cctacgacat gtccaagcat gacgactagt agccagaagc ccaagatgag
     2461 caaagaaaat gttttcattg cgtgccccgt gtccgcggat ccagccagcg atagatcaca
     2521 tcggttgcct ggttaatcag cacgtagcta aacgcgacca ccagcacaca agcctgggtg
     2581 accgggtaat cacgcttttc tatgctatct actaatagtc ggccaatgcc gttccagctg
     2641 aaaatggttt cagtgatcac agcacccgct aacaggctgc ccatttgcaa accaacgatg
     2701 gtgactaagg gcaataaagc ggcgcgtaag gcgtgtttaa gtaatacttg ccgctcactc
     2761 aggcctttgg cccgggccgt gcggatgtaa tcctcgttta atacttcaag caggctggtg
     2821 cgtgtcatgc gcgtcaaaat cgcgcttaac cccaacccca acgttagtgc aggtaacaaa
     2881 atggctgcac cactgcccat gccgctgact ggtaaccaac cgagccacac agcaaacacc
     2941 agcatcagta ccggccccaa ccagaacgcc ggcatggcgg ccaggcggat actcagcatg
     3001 gtaatcatca catcttgcca gtgcccagct tttaatgcag accagatgcc taatgggata
     3061 cctatcacca aacctattgc caatgcaacc aatgccagta acaaggtcgc tggataggcc
     3121 tgccatagca gttgtgagat gggcgtttgt gtgtggatag attgaccaaa atcacctcgc
     3181 agcaaatgcg acaagtactg cccgaattgc acgggcagag gctggtccag ccccaattga
     3241 gctttgattg cgacacgatc agcggggctg gcagattcac ccagcatgac atcaaccggg
     3301 tcgcccggta ccagatgcag caaggcaaag gtcaggcaca acaccccaaa gacgacaggt
     3361 agtaatgtag caaaacgctt aatcattcgc ctcgcaaata ttcacagtag tccctaccgg
     3421 caccaggtca aatagctcta tcaagtcagc gttacgcatg cgcacgcaac catgtgaacc
     3481 cggcacaccc agcacggttt catccggcgt gccatgaata taaatatagc gctgcatggt
     3541 atccacattg ccaagtcggt ttttacctgg ctcggtaccg ctcaaccaaa gaatgcgggt
     3601 taaaatccag tcgcgctctg ggaattgcac ctttaaatcc ggcgtgaaag tctccccggt
     3661 tggacggcgc ccgacaaaca ccgtattttc agcagcccca gcaccaattt tggcacgaat
     3721 gatgtgtgcg cccagtggcg tgcagccact gttcttaacg cagcctgccc cattagcggc
     3781 cgtcgatacg gcataccgtg tcacttgtcc gccattgtca tcaaacaagg tcagcgtttg
     3841 cgcagggatg gaaatttcaa tatgcatacc cgacattcta agcgatttgg ctgttgtcga
     3901 cggagatttg atgtaactca ctcgcaggct gcataaattc cattaaaatc gtcacatact
     3961 tcacttatca actccatctg gtagcttaaa tcagggttaa gcaatggcac atcacgaatt
     4021 acacttttta caacgcagtg gctggctgcg tgccgccgtg ttaggcgcca acgatggcat
     4081 tatttctacc gccagcttgc tgatgggcgt ggctgctgcc ggcgcaagcc accaggccat
     4141 gctaatgacc gggatcgccg gattagtcgc cggcgcgctc tccatggccg ctggcgaata
     4201 tgtctcggtc agttcgcaga cggatataga gcaagccgat ctcgcgcgtg aagcagaaga
     4261 attaaaggaa aaccctgaaa gtgaattgcg cgagctgacc ggtatttata tccagcgcgg
     4321 cttgtcacca gaactcgcca ggcaagtggc acaagcgcta tcgcaacgcg actcgctagc
     4381 cgcgcacgcg cgcgatgaat tgggtttaca tgacaacaat caggcacagc cactacaggc
     4441 agcactgaca tctgcgctcg cattcagtag tggtgcgctg ttaccaattg tcacggcctg
     4501 gctcgccccc cactcacagg cacaaacctg gctcaccatc agcaccctgc cctttttagg
     4561 tttgctcggc atggtctccg cccgcaccgg cggcgccaat gtattaaaaa gtgtcagccg
     4621 cgtggtgttc tggggggcgc tggcgctggc agccactgcg ctcattggcc gctggtttgg
     4681 cgtcaacccg tagcgccaag actttacacg gtaaaactag tcagctgcag gcttaaacac
     4741 gacctgcgtc aatccatccc agttaccatc aggcatcggc tggtaactgg caattgtatt
     4801 ctggtaagcg gtaaactggc cttcatacca cagcgggata acaggcaact gactatgcaa
     4861 acgagtggtc accgctggcc agttttcttt ttccagcagt tggtccaata cgggatcctg
     4921 gtagcgaccg cggttaaatc cttgcggcgg caggctatta gatgcaaaga ccttgctata
     4981 gatttcaggt gttttaatcc ctacccaagt caggccacac atttgaaaat tccctttttg
     5041 cacatccgca aaaaaagtgc cccagtccag gcttttgatt tgcaggtcta tccctgcttc
     5101 ctgcatttgc gcttgcaaaa tggttgccaa acgcacgcgc tgcgggtcgg tactggtttt
     5161 atagatgagg gtgagtggca attgaacacc ggcagactgc agcaactgcc tggccaatgc
     5221 tggctgataa cggtaaggct gcaactccgg gttacccgca taatgctctg gcggcaaaat
     5281 agcgttggct tcacgggtat ggctaaccat caccttatca ataatcgccc gcgtatcaat
     5341 ggcatgtgca atggcacgcc tgacacggac atcctttaaa aagtcagcct gcacattcag
     5401 acccagatag gagtaattag cacctatccc agtactcact gtcacatgag attgtttttg
     5461 caggtatttc accagctcag gcggcaaatc gccttgaatc aaatcggctt caccgcgttt
     5521 gagtttcaag acacgaacat tgggatcttt gacttcctcg aaacgcacct taaggccatc
     5581 agtgagtcgc gtgagctgta aagcagattg ccaacgatcc attttaaaag gtccgctgcc
     5641 tacgggtgca tgtccaaagt catgtccagc tgcaatcaac ctggcaggca aaataccaac
     5701 catcatcctt gctgcaaaat gcgggtctgg atgggagagc gtaaagtcta tggtgccagc
     5761 atctggtgtg gtgatgttct ggatatgcct gaattcggca gtatgcggag agtctttcag
     5821 ttgcagcaaa ctctcgtaag tcgctttgac atccaaagcg gtcaggggct gaccatcatg
     5881 aaacctggct gactgatgca attgaaaacg ataatgctgt gcatcctgca tttgccatgt
     5941 cgccagcccc ggcacctcgc gtgaactcgc atcaaactct accaggcgct ggtagatcag
     6001 ccggttaatc cgctctgaag ccgcatcagt cgcatagcgt ggatcaaggt tgacgggcgc
     6061 ttgtgctatc gcaaacacaa gcggatgcgg ctcatctgcg gactggcagc cagtcaatgc
     6121 aaccgttaat aatagccccg gcaaccaccg gtgcagcgcc agcgctacca cgttatgctt
     6181 tctcaaaaat cgcgatcgat tctacgtgag cagtgtgcgg gaacatattc atcacgccag
     6241 ctgcccgcaa gtgatagcct tttacttggg tcagaatcgc ggcatcgcga gacagcgtag
     6301 atggattaca agagacatag acgatacgcg atggcgcatg tcccgacgct gggtctccgg
     6361 catccggcaa ggctttaatc agttcaatcg cgccgtcgcg cggcgggtcc accaaccact
     6421 tgtcaaaagc acccagcgac tgcaaagatt gtgccgtcat atcaaacagg tcagactcgg
     6481 cgaactcgac gttttccaca ccatttaaac cggcgttttc tatagcacgt ttcactaacg
     6541 cctgagaacc ttcaacccca tagacactgg caccactacg cgcaattggc agcgtaaaat
     6601 tgcctagtcc acagaagaaa tcggcaatgc gttcgccggc ttttggctct aacaactgca
     6661 tcgcgcggcg gatcatgaca cggttaatgg ccggattcac ctgagtaaac tcggtcgggc
     6721 tgaagggata ctccagattg tattccggaa ggctatagtg cagcttgtcg ccatgttcaa
     6781 atagaggctt gacggtatcc ggacctttgg tttgtgtcca aacctgcgca ccatacgtct
     6841 cttcaaacgt ttgcatcaag gcaatatcgg cctcggtcag cgcctccaga atacggaaaa
     6901 ctaacacggt ggcatcttca cccacagcca attcaatctg cggcaatgac tgtttgatgg
     6961 atagctgacc gatcaactgt ttaagtggca caatgaggtc agacaccggt tgcgtcaata
     7021 cctcgcaact atccataaaa gtgacaaaac tgctgccctt ttcattaaaa ccaaccagca
     7081 cgccgccttt tttctcgaca taacgtacgc gtaagcgcgc tttatggcgg tagtgccatg
     7141 gtgtgccttg aataggtggc aggatttttt ccgggcgcac acggccgata tgccataaat
     7201 catcttctag catgcgctgc ttggcagcca cctgcgcaga aaactccaga tgctgcatgg
     7261 cacatccacc acaagcacca aaataggcac aacctggtgt cacacgctgg ctggacgctt
     7321 tgaccacctc taccgtggta gccagttcat aggtcgattt aacatggtga gagacaaacc
     7381 tgacctcttc aaaaggcaat gccccttcga taaaaatcac tttaccatcc acatgtgtga
     7441 caccacggcc ttcctgatcc agggattcaa taatggcggt tctgggaatc tgcgctgcct
     7501 tggcggcaga cgacaggttg gaggatcttt gacggttgtt tctcataacc tgttatttta
     7561 cctgaattgg gcatacaatc cctcaagaac ccaatcaaaa taaggagaac atgatgatca
     7621 aacaatgggg cctcgcgacc accctggcaa tcgcactttc ttcacccaac ttcagttttg
     7681 ccgacgaatt agctgtccag caggattccc acggaacaga ctacgtcact ggcggcatag
     7741 gctcggaaga ggttgatgcg atggaggtct ttaaaaagca attcaacctg tactttcttt
     7801 tttcggaggg taaggtagga cgcgtcattg atgacatcaa catcagcatc actgacagta
     7861 aaaaccagac tgtttttgct ttagagcatg cagcacccag attgctatta aacctgccaa
     7921 gtggcaaata tcaggcaact gccagttacc tcggcagcga gcaacgttac cgctttacgc
     7981 acgatgcgaa gaaacatcaa cgcattatcc tcaactggaa gaacaagatt gacgaagata
     8041 cgctagaggc tgccccagaa gatccggcag aagccaatgc acctacacct gaaacgcctt
     8101 aaatataccc acagaacacc agttttag
//
LOCUS       DATMSK010000264         9585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_69089,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000264 DATMSK010000000
VERSION     DATMSK010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69089"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..2484
                     /gene="clpA"
                     /locus_tag="VNZ84_08750"
     CDS             229..2484
                     /gene="clpA"
                     /locus_tag="VNZ84_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HWU67086.1"
                     /translation="MIAQELEVSLHMAFMDARQKRHELITVEHLLLAMLDNPTAAEVL
                     RACGAKFDTLRSELTQYIEEHTPTVAGTEEVDTQPTLGFQRVIQRAMLHVQSSGKKEV
                     TGANVLVAIYGEKDSHAVFFLHQQGVTRLDVVNFISHGVAKIQENEPKSASAEQETEG
                     ESVSTGALESFTLNLNAQVLAGKIDPLIGRGLELERVIQTLCRRRKNNPLLVGEAGVG
                     KTAIAEGLASRIVSNDIPEVLENATVYSLDMGALLAGTKYRGDFEQRLKAVMKQLAEK
                     PDAILFIDEIHTLIGAGSASGGTLDASNLLKPALSNGSLKCIGATTYQEYRGIFEKDH
                     ALSRRFQKIDVQEPSVAETIEILKGLKAKFEEHHSVKYSASALTVAAELSAKYINDRH
                     LPDKAIDVIDEAGAAQRILPKSKQKKIIGNKEIEEIIAKIARIPPKNISSDDRNALKT
                     LERDLKAVVFGQDKAIEVLASAVKMARSGLGNGNKPIGSFLFAGPTGVGKTEVAKQLA
                     YILGIELLRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAVTKQPYCVLLLDEIE
                     KAHPDIFNILLQVMDHGTLTDNNGRKADFRNVTIIMTTNAGAENMSKSSIGFTNSKDV
                     TDEAADLKRLFSPEFRNRLDATVSFSALNQEVILRVVDKFLMQLEDQLHEKKVDVTFS
                     DELKAYLGRKGFDPLMGARPMSRLIQDTIRKALADELLFGRLSEGGQVHIDIDDKDQI
                     QLIFKEETISA"
     gene            2697..3038
                     /locus_tag="VNZ84_08755"
     CDS             2697..3038
                     /locus_tag="VNZ84_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67087.1"
                     /translation="MQKIFYFLHALCFLFALQTTAAEPAVTTSLLETSIATASLSNAI
                     DDTDSLSADFGEVGGCVDMLEIAEVDVDKIVLPGFSYHLHKTIFLPPEHHLSAYHLEL
                     AFWLDRPPQSA"
     gene            3145..4389
                     /locus_tag="VNZ84_08760"
     CDS             3145..4389
                     /locus_tag="VNZ84_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWU67088.1"
                     /translation="MLSQSRWRKARKFLWLFCCLSPVAFAETTFDLKTLLDKAVAENP
                     SIAVSKAQTAAAAAGITVSRSYYNPDLEIMTGPSTARHADQREQQNYGIAISQPLDFM
                     STRASRREYAEQQFNYAEKGIHTTSFDLRNQIKAAYLNVLLRQQIMEMFEANLTLLQQ
                     VQSRVKRKVEIGEAAKYELIKADTEVLAAERDYASAKTRIDEAKAILRGMIGNMPDSF
                     ELTQQLPTISSLPDITELRRSAENNPYLQQLLSARDSAEAKLKLEQALRFPGITLKSD
                     FSQDPDINTVRMGVVVPLPVWNRREGQIAQAAAGIEEANANIKQQQLLLRKEIDRAYQ
                     LTVIASNQLKVFENGLLQQSQATLSRAEAAYKFGERGILEYLDAQRVNRDVKRDYLIA
                     KFDYFFSVLQIERFIGSDLVQP"
     gene            4400..5551
                     /locus_tag="VNZ84_08765"
     CDS             4400..5551
                     /locus_tag="VNZ84_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU67089.1"
                     /translation="MSRILFSTLLTAALAVMSSACSEKPAPAPATKEVVDPSLVTLTK
                     ELEANVKTGPVTEFQFVDTLRIPGRIQVDEQYESRAGASITGRISTLDVNLGDEVKAG
                     QRLATIKSTELAQYQLSYIKASQQMQLQTKAVDRAKQLLEADVISQAELQRREGELNA
                     ANAELNAARDQLLVLGMAPSSISQLGLHNSGMSTSQVNSKITGTVIARKARLGQVVAP
                     AEELFVIADLSHVWAVAEIPEQQIAHIREGQSLNIEVPALDDAELTGTLAFVGDIVNP
                     ATRTVMARANISNEKMLLKPDMLITILLEARPEKVVAVPASAVVRENNADHVFVKTMK
                     EKQYKLLPVTLGRTYQEHREVLQGLQPGDQIVLDGAFHVNNERKRKEME"
     gene            5554..8655
                     /locus_tag="VNZ84_08770"
     CDS             5554..8655
                     /locus_tag="VNZ84_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU67090.1"
                     /translation="MIESLIRSALKQRLIVLVLAIALIGAGLFAVQKLSVDAFPDVTN
                     VQVMIATQATGKSPEEVERFITVPLEIAMTGLPGLTEMRSVNKNALSLITLVFTDSTD
                     VYFARQLVMERLMEVMERMPPGVTPILGPVSTGLGEVYQFTLDKPSDDKKELTREDLT
                     ERRAIQDWVVRPLLRGIPGVAEINSQGGYVKQYQVLVNPDRMTHFSIKLKDVYEALAR
                     NNANSGGGILPHYAEQYLIRGVGLVQNLDDIRNMVLKEENGIPVYIRDVAEVTIGHEV
                     RVGVVLKNGYTESVGGIVIMMRGGNAKEVVSRIKARVEEINSKGMLPHGLQIVPYYDR
                     SELVDAALHTVTKVLIEGIILVIVILFLFLGDVRSSLIVVGTLVLTPLITFMVMNHYG
                     ISANLMSLGGLAIAIGLMVDGSVVVVENAFHHLGHRKNESKIRVVLEAASEVATPVLF
                     GVGIIILVFLPLMTLEGMEGKMFAPLAYTIAIALFVSLILSLTMTPALCSYVLKGGSG
                     EDTKLIQTIKKPYLSALNWVLNNEKKTITFSALLFIGTIALFPFLGTSFIPEMKEGSI
                     VPGINRVPNISLEESMKMENKAMKLIMEQVPGVKSAISGLGRGESPADAQAQNESTPI
                     ISLKPREEWPAGWSQDDIAEKMREVLEKYIPGVQIIMAQPISDRVDELLTGVRADVAV
                     KIFGEDLDTLKQKADEIARIAGTVKGATEIKVEKVSGQQYLNITIDRQAIARHGINTA
                     DVHDIVETAIGGKIATEIFEGQRRFSAAVRYPESFRNNEEAIRNIMLTSPNGSRVALE
                     DLADIEIKDGPAQISRELAKRRIVIGVNVKDRDLGGFVAELQQVLDQRLQLPDGYYLE
                     YGGQFQNMERAMGHLMIIIPVTIAAIFFLLFLLFQSIRFATMIILVLPFASIGGVIAL
                     FVTGEYLSVPASVGFIALWGIAVLNAVVLVSYIRTLREEGLDQLEAIRKGCAQRFRPV
                     MMTATVAMLGLVPFLFATGPGSEVQRPLAIVVIGGLITSTLLTLIVVPTLYRKFEESK
                     IEA"
     gene            8693..9025
                     /locus_tag="VNZ84_08775"
     CDS             8693..9025
                     /locus_tag="VNZ84_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWU67091.1"
                     /translation="MKEIKAVIQPARLPKIRSALRNIKGFPGMSVNKMEGCGPHLQKP
                     SVGVREELTDYSPKVYLYMVVPDELVEGILQTIVEVAHTGNIGDGIVWVTPVERMIRL
                     SEEIMVLD"
     gene            complement(9027..>9585)
                     /locus_tag="VNZ84_08780"
     CDS             complement(9027..>9585)
                     /locus_tag="VNZ84_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWU67092.1"
                     /translation="RYQIPVWLTHGTYSMCQRYLPAQALNIHIIDAHTTFALQEIEVQ
                     PYPVPHDAREPAQFVFSDGDCRLGVLTDVGSVTPHIVMMLQACDGLLLECNHDLDMLR
                     TGPYAHSLKKRVGGWLGHLDNQTSAQLLGQLDNSRLKHLVAAHLSEKNNTPALAKQAL
                     SDVLHCTTEWVQIASQQEGLDWRSL"
BASE COUNT         2538 a         2288 c         2425 g         2334 t
ORIGIN      
        1 acactagtac actagtggtt caattcctgt gtcctggtga tagcatgata agcaagtgtt
       61 tctggatcgc ttggctcatt taaacttgag taaaaagcaa gctcgaggca ttgaattttt
      121 tgcagaaaag cacgataaca ataaacagac aggttttgcc aaattgcagg aattacatct
      181 gtgaatgggc gttttgcagt ttagtgtagt gtattaagga aagtcatcat gattgctcaa
      241 gaacttgaag tcagcctgca catggcattt atggacgcgc gacaaaagcg tcacgaacta
      301 attacggttg agcatctatt actggctatg ttggataacc cgacagccgc agaagtcctg
      361 cgtgcctgcg gggccaaatt tgacacactg cgcagtgaat tgacccaata tattgaagaa
      421 catacaccta ccgtcgctgg tactgaagaa gtagatacac agccgaccct gggcttccaa
      481 cgcgttattc agcgcgccat gctgcacgtg cagtcttctg gtaaaaaaga agtgactggc
      541 gcgaatgtcc tggttgccat ctacggtgaa aaagactcgc atgccgtatt tttcctgcat
      601 cagcaaggcg tcactcgcct ggatgtcgtg aacttcatct cgcatggcgt ggcgaaaatc
      661 caggaaaatg aacccaagtc tgcaagcgcc gaacaagaga cagaaggcga aagtgtcagc
      721 acgggggcct tggaaagctt caccttaaac ctgaacgcac aagtgctagc gggcaagatt
      781 gacccattga tcggtcgcgg ccttgaactg gagcgcgtga tccagacact gtgccgtcgc
      841 cgcaagaaca accctttgct agtgggcgaa gctggtgtgg gtaaaactgc gattgcagaa
      901 ggacttgcct cgcgtatcgt cagcaatgac atcccggaag tgctggagaa tgcgactgtc
      961 tattcactag atatgggcgc cctgctggca ggcaccaaat accgtggtga tttcgagcaa
     1021 cgcctgaaag cagtcatgaa acaactggca gaaaaaccag atgccatcct gtttattgac
     1081 gagattcata cgctgattgg tgctggttct gcaagcggcg gtacattgga cgccagcaac
     1141 ctgctcaaac cagccttatc caatggctca ctgaaatgca taggtgcaac cacctatcag
     1201 gaatatcgtg gcatatttga aaaagaccat gccttatcac gccgtttcca gaaaatcgac
     1261 gtgcaggagc ctagtgtggc cgaaaccatt gagatcctca aaggcctcaa agccaaattt
     1321 gaagaacacc acagcgtaaa atatagcgcc agtgccttaa ccgttgctgc tgagttgtca
     1381 gccaaataca tcaatgaccg ccatttgcca gacaaggcaa ttgacgtcat tgatgaggca
     1441 ggtgctgcgc aacgtatctt gcctaagagc aagcagaaga aaatcattgg caacaaagag
     1501 attgaagaaa tcattgccaa gattgcacgc attccaccca aaaacatttc cagtgatgac
     1561 cgcaacgcac tgaaaacatt ggagcgtgac ctgaaagccg tggtatttgg ccaggacaaa
     1621 gccattgagg tattggcctc agctgtgaaa atggcacgta gcggactggg taacggcaat
     1681 aagccgattg gtagcttcct gtttgctggc ccaaccgggg ttggtaaaac tgaagtggcg
     1741 aaacaactgg cctatatcct tggcattgag ttgttgcgct ttgacatgtc cgaatacatg
     1801 gaacgtcatg cggtgtctcg cctcatcggc gcgcctccgg gctatgtcgg ctttgaccag
     1861 ggtggtttac tgactgaagc agtgactaag cagccctatt gcgtgttgct gctggatgag
     1921 attgaaaaag cccatccgga tatcttcaat atcctgctgc aagtgatgga ccacggcaca
     1981 ctgacggata acaatggccg caaagcggat ttccgtaatg tgacgatcat catgaccacc
     2041 aatgccggcg cagaaaatat gtcgaaatcc agcataggct tcaccaatag caaagatgtg
     2101 acggacgaag cggctgacct gaagcgcttg ttcagtcctg agttccgtaa ccgcttggat
     2161 gccactgtgt cattcagcgc actgaaccag gaagtgatct tgcgtgtcgt cgacaaattc
     2221 ctgatgcaac tggaagatca gttgcacgag aaaaaagtcg atgtgacctt tagtgacgag
     2281 ctaaaagctt atctgggtag aaaaggcttt gatccattga tgggcgcacg tcctatgtcc
     2341 agactgattc aggataccat tcgtaaagca ctggcagatg aattgctgtt tggccgcttg
     2401 agtgagggcg gacaggttca catcgatatt gatgacaagg accagatcca actaatcttc
     2461 aaagaagaga ccatttcagc ataatctgtt ttaactttaa caccatagtg gcatctaccg
     2521 gatgccacta tgtctttaag ggttatccgc actggtttag tgcaataagc agtcccacct
     2581 tcctgcatct gcgccagaca aactgcggat cagcataaaa tcaatatgtt aggtgcggcc
     2641 gcattgctgg tataattatt gctgtaaatg gattgcctga cagatctgac ttgtacatgc
     2701 aaaagatttt ttattttcta catgcgttgt gttttttgtt tgcgttgcaa acaacggctg
     2761 ccgaaccggc ggtcacaact tcgcttttag aaaccagcat cgcaaccgct agcctttcca
     2821 acgcgattga tgatactgac agcctctccg ccgattttgg cgaagtgggg ggctgtgtgg
     2881 acatgctgga aatcgctgaa gtcgatgtcg acaagatcgt attgccaggc tttagctacc
     2941 acctacacaa aaccatcttc ctccctcctg aacaccactt atctgcctac catctggaac
     3001 tggctttttg gttagaccgg cctccccaat ccgcttaacg aagattctca atctccacgc
     3061 ttgtctggct ggagtattat tttaattatt aattcaggtg attgtgacat gatgacatga
     3121 acaatcacct aaaaaaggat cgttatgtta agtcagtccc gctggcgaaa ggcacgtaag
     3181 tttttgtggc tattttgctg cctctctcct gtcgcgtttg cagaaaccac ttttgaccta
     3241 aaaacattat tagacaaagc cgttgcagaa aacccttcta ttgctgtaag caaagcgcaa
     3301 acggctgctg ccgctgccgg gattaccgtt tcacgctctt actataatcc ggacctagag
     3361 atcatgacgg gtccatccac agcccgtcat gcagatcagc gcgagcagca aaattacggc
     3421 attgcaatct cccagccatt ggactttatg agtacgcgtg cgtcgcgtcg cgagtatgct
     3481 gagcagcagt ttaattacgc tgaaaaaggt atccatacca ccagctttga tctgcgtaac
     3541 caaattaaag ccgcttactt aaatgttctg ctgagacagc aaattatgga aatgtttgag
     3601 gcaaacctga ccttactgca acaggtacaa agccgggtga aacgcaaagt cgagataggt
     3661 gaagccgcaa aatacgagtt gattaaggca gatactgaag tgctggcagc cgagcgtgac
     3721 tatgccagtg caaaaaccag aattgatgaa gccaaagcaa ttttgcgtgg catgataggt
     3781 aatatgccgg attcgtttga gctcacgcag cagctaccga ccatctctag cctgcccgat
     3841 atcactgagt taagacgttc ggccgagaat aatccttatc tgcaacaact ccttagtgcc
     3901 agggacagtg cagaagctaa actcaaactg gagcaggcgc tgcgctttcc agggataacc
     3961 ttgaaatcag acttctcaca agaccctgac atcaacaccg tcagaatggg tgtcgtggtt
     4021 cccttaccag tgtggaaccg ccgcgaaggc cagattgcac aggccgcagc agggattgaa
     4081 gaagcgaatg ccaatatcaa gcagcaacaa ttactgctac gtaaggaaat agacagagct
     4141 taccaactta cagtgattgc cagcaaccag ctaaaagtgt ttgaaaacgg gctgttgcag
     4201 caatcacaag cgaccctgag tcgtgcagaa gccgcttata aattcggaga acgcggcatc
     4261 ctggaatacc tggatgcgca acgcgtcaat cgtgatgtca aacgtgacta cctgattgcc
     4321 aaattcgatt attttttcag tgtattgcaa atcgaacgct tcatcggttc tgatttggtt
     4381 caaccttaag gggatggtga tgtctcgtat tttattttca accttgctga cggcagcact
     4441 cgccgtcatg tcctcagcgt gcagcgaaaa accagcacca gctcccgcaa ccaaagaagt
     4501 cgttgaccct agtctggtga ccctgactaa agagctggaa gccaacgtta aaaccggccc
     4561 cgtgacagag tttcagtttg tcgacaccct gcgcatccct ggccgtatcc aggtcgacga
     4621 acaatacgaa tccagggctg gcgcatcgat cactggccgc attagtacac tggatgtgaa
     4681 tctgggcgat gaagtgaaag ccggccaacg ccttgcaacc attaaaagta ctgaactggc
     4741 gcaatatcaa ttgagctaca ttaaagccag tcagcaaatg cagttacaaa ccaaagcggt
     4801 ggaccgggct aaacagttgc tggaagccga tgtcatcagc caggccgagc tccaacgccg
     4861 cgaaggtgaa ctcaacgccg cgaatgctga attgaatgcc gcacgagacc agttactcgt
     4921 actcggcatg gcaccctcaa gcatttccca gcttggcttg cacaacagtg gcatgtctac
     4981 cagccaggtt aactccaaga tcaccggcac agtcattgcg cgcaaggcca gattggggca
     5041 ggtcgtagcg ccggctgaag aattattcgt gattgcagac ttgtcccacg tgtgggcagt
     5101 cgcagaaatt ccggagcagc aaattgcaca tatcagggaa ggccaatccc tgaatattga
     5161 agtccctgca ctggatgatg ccgaactgac aggcacgctt gcatttgttg gcgatattgt
     5221 gaatccggca acccggacag tgatggcccg cgcaaacatc agcaacgaaa aaatgctgct
     5281 aaagcctgac atgctgatca ccattttact ggaagcaaga ccggaaaaag ttgtggccgt
     5341 gcctgcttca gcggttgtgc gcgaaaacaa tgccgaccac gtctttgtga aaacgatgaa
     5401 ggagaagcaa tacaaattgc tgcctgtgac cttgggacgc acctatcagg aacacaggga
     5461 agtgttacaa ggcttgcaac caggtgacca aatcgtgctg gatggcgctt ttcacgtgaa
     5521 caacgagcgt aagcgcaaag aaatggagta agcatgattg agtctttaat tcgaagtgcg
     5581 ctcaagcagc gtctgatcgt acttgtgctc gctattgcac tgattggtgc tggtttattt
     5641 gctgttcaaa aactgtccgt cgatgcattc cctgacgtga ctaacgttca agtcatgatt
     5701 gcgacacaag ccactggaaa atctccggaa gaagtggagc gttttatcac ggtcccactc
     5761 gaaattgcaa tgacgggcct cccaggcttg actgaaatgc gctcggtgaa caagaacgcc
     5821 ctgtcactga ttacgttggt attcaccgac agtaccgatg tctattttgc tcgccaattg
     5881 gtgatggaac gtcttatgga agtgatggag agaatgcctc caggcgtgac tcccatcctt
     5941 ggtccagttt ctaccggctt gggcgaagtc tatcaattca cactagacaa gcccagcgat
     6001 gacaagaaag agctgacacg agaggacctg acagaaaggc gcgccattca ggattgggtt
     6061 gtgcgtccgt tactgcgtgg catccccggt gttgctgaga ttaactctca aggcggttac
     6121 gttaagcaat atcaggtact agtcaatccg gaccggatga cgcatttctc aatcaagctc
     6181 aaagacgttt atgaggcgct agcccgcaat aatgccaata gtggcggtgg catactgcct
     6241 cattatgctg agcaatatct gatccgtggc gtgggcctgg tgcaaaacct ggacgacatc
     6301 cgcaatatgg tattgaagga ggaaaacggc atcccggtgt atatccgcga tgttgccgag
     6361 gtgacaatag gccatgaagt gcgtgtcggt gttgtcctca aaaatggcta caccgaatct
     6421 gtaggcggta ttgtgatcat gatgcgtggt gggaacgcca aagaggtggt cagtcgtatc
     6481 aaagcgcgag ttgaagaaat caacagcaaa ggcatgctgc cacacgggtt gcaaatcgta
     6541 ccctattacg accgtagcga attggtggat gcagcactgc atactgtcac caaagtgctg
     6601 attgaaggca ttatcctggt catcgtcatt ctttttctgt tcttggggga tgtccgttcc
     6661 agcctgattg tggttggtac cctcgtgctc acgcccttga taacctttat ggtgatgaat
     6721 cattatggga tttctgccaa cctgatgtca ctgggtggtt tggctattgc catcggcctg
     6781 atggtggacg gttcagtggt cgtggttgaa aatgcatttc accaccttgg tcaccgcaag
     6841 aatgaaagta aaatccgcgt cgtcctcgag gctgcgagtg aagtcgcaac acctgtatta
     6901 tttggtgtag gcattattat tcttgtgttc ctgccattga tgacgcttga aggcatggaa
     6961 ggcaaaatgt ttgccccgtt ggcatatacc attgccatcg ccttgtttgt gtcactgatc
     7021 ctatctttaa ccatgacacc tgcgttgtgc tcttatgtgt tgaaaggcgg tagtggtgaa
     7081 gataccaagc tgatacagac tattaagaaa ccttacttgt cagccttgaa ctgggtgctt
     7141 aataacgaga aaaaaacaat tactttctca gcattattat ttattggcac gatcgcgctg
     7201 ttccccttct taggtacttc ttttattccg gaaatgaaag agggctcaat cgttccaggc
     7261 attaatcgtg tgcctaacat ttcgcttgaa gagtccatga aaatggagaa caaggcgatg
     7321 aagctgatta tggaacaagt gcctggtgtc aaatccgcca tctcgggtct tggtcgtggc
     7381 gaaagcccag ctgacgcaca ggcgcaaaac gaatcgactc cgattatcag cctgaagccg
     7441 cgtgaagaat ggccggcagg ctggagccaa gatgatattg ccgaaaagat gagagaagta
     7501 ctggagaagt atatcccagg tgtgcaaatc atcatggcgc aaccgatttc tgaccgggtg
     7561 gatgaattgc tgactggcgt tcgtgctgac gtcgctgtca agatatttgg tgaagacctg
     7621 gataccttga agcagaaagc ggacgaaatt gcgaggattg ctggtacggt aaaaggtgct
     7681 accgaaatca aagtagaaaa agtctctggt cagcaatacc tgaatattac gattgaccgt
     7741 caggctattg cgcgccatgg cattaataca gcggatgtgc acgatatcgt cgaaacagcg
     7801 attggtggca aaatcgccac cgagattttc gaaggtcaac gcaggttttc tgccgcggtg
     7861 cgttacccgg agtctttccg caataatgaa gaagccatcc gtaacatcat gttgacttca
     7921 cccaatggtt ctcgagtcgc tcttgaagat ctagcagata ttgaaatcaa ggatggtcca
     7981 gcccagatca gcagagagct ggccaagcgt cgtatcgtca ttggggtaaa cgtcaaagac
     8041 cgcgacttgg gtggtttcgt agctgaactg caacaagtgc ttgatcagcg cctgcaattg
     8101 ccagatggtt attatctgga atatggtgga cagttccaga atatggagcg agcgatggga
     8161 cacttgatga ttattattcc ggtgacgatt gcagcgatct tcttcctgct attcctgttg
     8221 ttccagtcga tacgttttgc caccatgatt atcctagtac tgccatttgc atcgattggt
     8281 ggcgtgattg ctttgttcgt cacaggggaa tatttatctg tcccggcatc tgttggcttt
     8341 attgccttat ggggcattgc cgtccttaat gccgtggtcc tggtgtctta tatccggacc
     8401 ctgcgcgaag aaggtcttga tcaattggaa gcgatccgca agggctgtgc ccagcgtttc
     8461 cgcccggtga tgatgacagc cacggtggcc atgctaggcc tggtcccctt cttgtttgcg
     8521 accggcccag gctctgaagt tcagcggcca ttggcgattg tcgtgattgg cggactaatt
     8581 acctctaccc tactcacgct tattgtggtg cccaccttgt accgtaagtt tgaagaaagc
     8641 aaaatagagg cataatccaa ggttaacgct gattaaagcg aaggatcaat ttatgaaaga
     8701 aattaaagcg gtcatacaac cggcgaggtt acccaaaatt cgctcggcat tacgtaatat
     8761 caagggtttc ccaggcatgt ctgtcaacaa aatggagggt tgtggcccac atttgcaaaa
     8821 acctagtgtc ggggtccgcg aagaactgac tgattacagt cccaaagtct acctttacat
     8881 ggtggtaccg gatgagctgg ttgaaggcat cttgcaaacc attgtggaag tcgcacacac
     8941 tggcaatatt ggtgatggca tcgtttgggt cacgccggta gaacgtatga tccggttatc
     9001 agaagaaatt atggtgcttg attaaatcat aaagaacgcc agtccagccc ctcttgctgg
     9061 ctggcaatct gcacccactc tgtggtgcaa tgcaaaacat cactcagcgc ctgctttgcc
     9121 aaagcaggcg tattattttt ctcactcaag tgcgctgcca ccagatgctt gagcctgctg
     9181 ttatccagct gacctaataa ttgcgcactg gtctggttat ccaaatgtcc cagccaaccg
     9241 ccaacccgtt ttttaagtga atgcgcatac ggcccggtcc gcagcatgtc taggtcatga
     9301 ttacattcga gtagcaaacc atcgcaggct tgcagcatca tgacaatatg cggtgtcacg
     9361 ctaccgacat cggtcagcac gcctagccgg cagtcaccgt cagaaaacac aaactgggcg
     9421 ggctcacgtg catcatgcgg gacaggataa ggctgcactt caatttcttg caacgcaaat
     9481 gtcgtatgcg catctatgat atgaatgttg agggcttgag ccggcaggta gcgttggcac
     9541 atgctgtaag tgccatgtgt tagccagacc gggatttgat aacgt
//
LOCUS       DATMSK010000265         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_91413,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000265 DATMSK010000000
VERSION     DATMSK010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91413"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VNZ84_08785"
     CDS             <1..529
                     /locus_tag="VNZ84_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWU67093.1"
                     /translation="LKGIADNDPKLEPLIASLASSPLPRVQRTAIYMVSNLDTVPQAL
                     MHNLKHNQVLHQRNLIVNVSFTDVPFMNAHERVTVQRLAEGFWQVQLRYGFMQHADIP
                     QALLQLSLDGASLDPFTNSYFLSRDIIVPDVGGAMPRWRDQLFSVMSRNAVSAVEYFN
                     IPANSVIELGSRVQL"
     gene            complement(581..>2120)
                     /locus_tag="VNZ84_08790"
     CDS             complement(581..>2120)
                     /locus_tag="VNZ84_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWU67094.1"
                     /translation="RGEADNGQYKRIGKGGKEVWLQASYNPIFDASGKPFKVVKYATD
                     ITAEKLKNSDFEGQISAISKVMGVIEFDLKGNITNVNPNFAAVTGYSPQEIVGNHHSM
                     FVESGYKTSQEYKQFWEKLARGEPDNGQYQRVGKGGKVVWLQASYNPILDAEGNAFKV
                     VKYATDITAKVNEAKAMENAVHETGEVVGLVQSGDLTQRISTHDKNGEIKELCEGVNS
                     IVDTMADILASIKVAGETINTAANEISVGSTDLSQRTEEQASSLEETAASMEEISSTV
                     KQNAQNAKQANTMTFKAGEIATRGGEAFGRVVTTMSAITESSARIEEIITVIDSIAFQ
                     TNILALNAAVEAARAGEQGRGFAVVASEVRNLAQRSASAAKEIKQLIVDSAEKVANGS
                     MYVDQANGTMDEIIIAIQQVSDLISEITSASQEQTTGIDQVNLAVTQLDEVTQQNAAL
                     VEEAAAAAMSLVDQAAQLNAKVSQYKLGNAVSSVPRQRSTAQPARRVTARTGTDNNEE
                     WEAF"
BASE COUNT          441 a          569 c          528 g          582 t
ORIGIN      
        1 attgaaaggg atcgcagaca atgaccccaa gcttgaaccc ttgatcgcca gcctggccag
       61 cagcccatta ccacgcgtgc aaagaaccgc catttacatg gtttccaacc tggatacggt
      121 accgcaagcc ttgatgcata acctcaagca taaccaggtg ctgcatcaaa gaaatttgat
      181 cgtgaatgtc tcttttacag acgtgccctt tatgaatgcg catgagcgcg tgacggtgca
      241 gcgattggca gaaggattct ggcaagtgca attgcgttat ggattcatgc agcatgcgga
      301 cattccgcaa gccttgttgc agctatctct ggatggcgcg tctttggacc catttaccaa
      361 ttcctatttc ctgagtcgcg atattattgt gccggatgtc ggtggcgcta tgccgcgctg
      421 gcgggatcag ttgttctcgg tgatgtcgcg caatgcggtc agcgccgtgg agtacttcaa
      481 tatcccggcc aacagtgtga ttgagctagg gagccgggtg cagttgtagt caaaaaaaca
      541 ccccgcctag acggggtgtt ctgctcgaaa gatgatgtgt ttaaaaggct tcccactctt
      601 cgttgttatc cgtgccggtg cgcgcggtca cgcggcgggc aggctgcgcg gtagaacgtt
      661 gtctgggcac actgcttact gcattaccta gcttgtactg gctcactttg gcattcaact
      721 gggctgcctg atccaccagt gacatcgcag cggcagccgc ttcttcaacc aaggcagcgt
      781 tttgctgggt cacttcatcc agttgcgtca ctgccagatt cacctggtca ataccggttg
      841 tctgttcctg cgaggctgag gtgatttcgc tgatcaaatc agacacctgc tgaatcgcaa
      901 taatgatttc atccatggtg ccgttggcct ggtccacata catagagcca ttggccactt
      961 tttctgccga gtccacaatg agctgtttga tttccttggc agcactggcc gaacgctgcg
     1021 ccaggttgcg cacctcgctg gctaccacgg caaaaccacg tccttgttca ccggcacggg
     1081 cagcttctac cgccgcgttc aaggccagga tattggtctg gaaggcaatg ctgtcaatca
     1141 cggtaataat ttcttcaatg cgggcggagc tttcggtaat cgctgacatg gtagtgacca
     1201 cgcggccaaa cgcttcgccg ccacgggtgg cgatctcacc agccttaaag gtcatggtat
     1261 tggcttgttt ggcattctgc gcattctgtt tcacggtcga agaaatttcc tccatgctgg
     1321 cggctgtttc ttcgagtgag ctggcttgtt cttcggtgcg ttgcgacagg tctgtgctgc
     1381 ctaccgaaat ttcgttggca gccgtattaa tggtttctcc ggccacttta atcgatgcca
     1441 gtatatccgc catggtgtcc acaatactgt tcacgccttc gcataactcc ttgatctcgc
     1501 cgttcttatc atgggtgcta atacgctggg tcaaatcgcc gctttggact aaaccaacga
     1561 cctccccagt ttcatgcacg gcattttcca tggctttggc ttcattcact ttagccgtaa
     1621 tatcggtggc atatttcacc accttaaagg cattgccttc cgcatccaga atcgggttgt
     1681 agctggcttg caaccaaacc accttgccac ctttacccac acgctggtac tgaccattat
     1741 caggttcacc gcgtgccagt ttctcccaga actgtttgta ttcctggcta gttttgtagc
     1801 cggattccac aaacatgctg tgatgattgc caacgatttc ttgtgggcta tagcctgtca
     1861 ccgcagcaaa gttagggtta acgttggtga tattgccttt caggtcaaac tcaattacgc
     1921 ccatgacttt gctaatagca gaaatctggc cttcaaaatc tgagttcttg agtttttcag
     1981 cggtgatgtc agtcgcatat ttaaccactt tgaacggctt gcctgaggcg tcaaaaatag
     2041 ggttgtagct ggcttgtaac cagacttctt taccgccctt gccgatacgt ttgtattgac
     2101 cattatctgc ctcgccacgg
//
LOCUS       DATMSK010000266         9074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_280813,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000266 DATMSK010000000
VERSION     DATMSK010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280813"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2650)
                     /locus_tag="VNZ84_08795"
     CDS             complement(<1..2650)
                     /locus_tag="VNZ84_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HWU67095.1"
                     /translation="MSKFQLKKIAFALSLSTALTVHAAGLGSMISSSKLGEPLNAEIE
                     LLAVTPGELSSIQAALASEQVYQDQMLEKPASYPFIKIEVGNNSKGQPILKLSSTQPI
                     TEAFLDMLIQVDWPTGRLVKEYTLLLDPPGFNSNYVSESAGLPVTSQTAPTTTENTSA
                     AAMSNEPSQPVVTAPKKPAKSVAEKPAPKATQDESSETPVNALTTERGDTLYAIARQM
                     KPDNVSTEQMLAALYQSNQQAFDGKNMNRLKVGKIIRMPDQATLDSISRPQARSLVAE
                     HTSNWQAYKNSLAKVVKESAPSNAGGNTQQSAGKIGSASEKPVPKAKDAKDVLKLSAG
                     DEKSTTQADKAAAKATAAQEDAIAKENAIKEEQSRAAALEKQVADMKKLMEMKNSAMA
                     QAEKNAAQAAEPKDEAKTVPEPAAHEKTAEQVPVPSKQDKTVTEQPPAPPVAAPTSDE
                     AVQPPSFMHVMLERLKKINPVLPWALIALPLLLCICALIRGRRKKQIDTFEDVIVTSP
                     ATEMQNNTVFGDTQAAASGDTSFLTDFSQSGVGGMIDSHDVDPIAEAEVYMAYGRDAQ
                     AEEILKDAIQKDPERQELKLKLLEIHQATANKGAFESLASEIYAKVGPADATWSKVTA
                     MGQKLDPENSLYQSTPADTASAPAAQAAELPVTEAQAEDEGYHFNFETPALEFDGASE
                     AFAAASAAPVDANDTAAPESIKLNTVADAEGDLQPSSGDADILQEEPQIAPPQQQPES
                     ESTLVFSSDNADTLVSDSADVARFEVEPSNTAVESDEVSLGGGLELTPKSLDLPSLNL
                     DEGIDATDAGATKLSQPATGEPLLSSEDAVFESLPELSLELGGPNDGTSPDDEALDIE
                     EISLDSPVDALNEIEAPSAPAEEVEFP"
     gene            complement(2859..3971)
                     /gene="asd"
                     /locus_tag="VNZ84_08800"
     CDS             complement(2859..3971)
                     /gene="asd"
                     /locus_tag="VNZ84_08800"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWU67096.1"
                     /translation="MLKVGFVGWRGMVGSVLMQRMMQENDFADIEPQFFTTSQTGGAA
                     PKVGKESPALKDAKSVDALRQMDVIVTCQGGDYTSEIFPQLRATGWNGHWIDAASTLR
                     MEKDAVIILDPVNMHVIKDALVDGGKNWIGGNCTVSLMLMALNGLFKADLVDWATSMT
                     YQAASGAGAQNMRELISQMGVINASVADLLADPASAILQIDKTVADTIRSEDLPQSNF
                     GVPLAGSLIPWIDKDLGNGQSKEEWKGGVETNKILGREANPIVIDGLCVRIGAMRCHS
                     QALTIKLRKNVPLDEINQMLTEANDWAKVIPNEREISMRELTPAAITGTLTTPVGRLR
                     KLAMGGEYLSAFTVGDQLLWGAAEPLRRMLRILVEA"
     gene            complement(4017..4136)
                     /locus_tag="VNZ84_08805"
     CDS             complement(4017..4136)
                     /locus_tag="VNZ84_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HWU67097.1"
                     /translation="MTKWMTLVLAVLVLTGCNTINGMGKDIEQAGEAVQKSSK"
     gene            complement(4153..4647)
                     /locus_tag="VNZ84_08810"
     CDS             complement(4153..4647)
                     /locus_tag="VNZ84_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HWU67098.1"
                     /translation="MPSFDITSEVDMVNLKNSIDASVKQITNRYDFKGTSAKVEFNEK
                     DKVITLFGDNDFQLDQVKDILLPAMEKKEPESSKRLEHKDVQKVSGNKVKQDLRVQDG
                     IDSDLAKKITKLIKDSGMKVQASIQGDTVRVTGAKRDVLQEAIAFVKKSITDFPLKFG
                     NFRD"
     gene            complement(4748..5059)
                     /locus_tag="VNZ84_08815"
     CDS             complement(4748..5059)
                     /locus_tag="VNZ84_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HWU67099.1"
                     /translation="MAQFDNVSVKKKANVYFDGKCVSHTVLFPNGTRCTVGVIFPSTL
                     TFNTGSPELMEINDGVCKVRQKDEEHWTTYKGGEKFTVPGNSSFDIEVIETVDYVCHF
                     E"
     gene            complement(5145..6215)
                     /gene="leuB"
                     /locus_tag="VNZ84_08820"
     CDS             complement(5145..6215)
                     /gene="leuB"
                     /locus_tag="VNZ84_08820"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWU67100.1"
                     /translation="MALNIAVLPGDGIGPEIIAQALRVLEVLKKEGLDMTFTEAPLGG
                     QAYDQFGHPYPEFTQEICRKADAVLLGAVGGPQYDTLDRPLRPERGLLAIRKDLGLFA
                     NLRPAVLFPELANASTLKPEVVAGLDIMIVRELTGDVYFGQPRGMRTNEQGVREGFNT
                     MIYSEPEVRRIAHVAFDIAMKRGKKLCSVDKANVLETTEFWKEIVIDVAKEYPEVELS
                     HMYVDNAAMQLIRNPKQFDVMVTGNIFGDILSDEASMLTGSIGMLASASLNESKKGLY
                     EPSHGSAPDIAGKNLANPIATILSAAMMLRYSFDNEAAATRIENAVKKVLAAGYRTGD
                     IYEEGTKKVSCSEMGDQIVAAM"
     gene            complement(6309..6947)
                     /gene="leuD"
                     /locus_tag="VNZ84_08825"
     CDS             complement(6309..6947)
                     /gene="leuD"
                     /locus_tag="VNZ84_08825"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWU67101.1"
                     /translation="MKAFTTLNGLVCPLDRANVDTDAIIPKQFLKSIKRSGFGPYLFD
                     EWRYTNYGEPGMDCSTRPLNPDFVLNQPRYQGAQVLLARDNFGCGSSREHAPWAIEDQ
                     GFRVIIAPSFADIFFNNCYKNGILPIVASAEQVDNLFKECFANEGYTLNVDLEAQIVT
                     TSSGQSFSFDITPTRKHNLLNGLDEIGLTLQHADKIKAFEEKHKASQPWLFA"
     gene            complement(6960..8366)
                     /gene="leuC"
                     /locus_tag="VNZ84_08830"
     CDS             complement(6960..8366)
                     /gene="leuC"
                     /locus_tag="VNZ84_08830"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWU67102.1"
                     /translation="MAGKTLYDKLWESHVVREDTDGTCLIYIDRHLVHEVTSPQAFEG
                     LRMAGRKPWRNSSIVANPDHNTPTKDWDKGIEDPISRLQVETLDNNIKEFGALVYFPF
                     KNKDQGIIHVIGPENGTTLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     VAKKSKSMLVRVDGQLGKGVTAKDVALAIIGKIGTAGGTGYAIEFGGEVIRGLSMEGR
                     MTLCNMAIEAGARAGLVAPDEKTAAYLEGRLHAPKGDNWTKAVAYWKTLSSDVDATFD
                     TVVVLNGAEIAPQVTWGTSPEQVLPIGSTVPDPAQEADATKRTSIENALKYMGLTANT
                     PIDQIQLDKIFIGSCTNSRIEDLRAAASVAKGKKVAGNIKLAMVVPGSGQVKRQAEAE
                     GLDKIFIEAGFEWREPGCSMCLAMNSDRLSPGERCASTSNRNFEGRQGPGGRTHLVSP
                     EMAAAAAIAGHFVDVRSA"
     gene            8486..>9074
                     /locus_tag="VNZ84_08835"
     CDS             8486..>9074
                     /locus_tag="VNZ84_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU67103.1"
                     /translation="MDTRRQELAEFLQAMRQRGTPEEFGFPSGSRRRTQGLRREEVAQ
                     LAGISATWYTWIEQAREVNVSAEALDRLATALKLSKSERSYLFDMADRRDPQAHQSEV
                     DTAPETLVTMLSQIQIPAYVMGRTWDILAWNTPAGHLFTGLLDIAWPESQRPNLLRFV
                     FANPQARQFVVNWEMRARRLVAEFRADCRSRLEEPE"
BASE COUNT         2151 a         2436 c         2082 g         2405 t
ORIGIN      
        1 cgggaaactc cacttcttca gcaggcgctg agggggcttc tatttcattt aaagcgtcga
       61 ctggtgagtc cagactaatt tcctcgatgt ccagcgcttc atcatctggg cttgtcccat
      121 cattcggacc acccagttcc agagaaagct ccggcagaga ttcaaacact gcatcctcac
      181 tcgacaacaa gggctcgcct gtggcaggtt gcgatagttt agtagcgcct gcatccgttg
      241 catcaatgcc ttcgtccaga tttagggaag gcaaatccag ggacttgggc gtgagctcca
      301 gaccccctcc cagagacact tcatcactct caactgcagt gttagatggt tccacctcaa
      361 agcgcgccac atctgccgaa tccgacacca gagtatccgc attgtcagaa gaaaagacca
      421 gggttgattc actctctggc tgttgctgtg gtggagcaat ctgcggctct tcctgcaata
      481 tatcggcatc tcccgagctg ggctgtaagt caccttctgc atccgctact gtgtttagct
      541 tgatggattc tggagcagct gtgtcattgg cgtccacggg cgctgcgctg gcagctgcaa
      601 acgcttctga agccccatca aactctagag ccggggtttc aaaattgaaa tgatagcctt
      661 catcttccgc ttgcgcttcg gtcacaggta actctgcggc ttgcgccgct ggtgcgcttg
      721 cagtatctgc cggagtcgat tgatacaatg aattttctgg atccagtttt tgtcccattg
      781 cggtgacttt tgaccaagtc gcatcagctg ggccgacttt ggcataaatc tcgctggcca
      841 gtgactcaaa cgcacccttg ttcgcagtcg cttggtgaat ctccagcaat ttcaatttaa
      901 gttcctggcg ttccggatct ttctggatgg catctttaag aatttcctca gcctgggcat
      961 cacggccgta cgccatatat acctcagcct cagcgatcgg atccacatca tgtgaatcaa
     1021 tcatgccgcc gacgccactt tgcgagaagt ccgtcaggaa ggaggtgtcg ccagaagctg
     1081 ctgcctgagt atcaccaaag actgtgttat tttgcatctc agtcgccggg gaggtcacga
     1141 tcacgtcttc aaaggtatca atctgctttt tacggcgccc cctgatcaat gcgcaaatac
     1201 aaagcaatag cggcaatgcg atcaaggccc atggcaatac cgggttgatc ttcttaaggc
     1261 gctcaagcat gacgtgcata aatgaaggcg gctgcaccgc ttcatccgag gtaggcgctg
     1321 caactggtgg tgctggtggc tgctcggtaa cagttttgtc ttgcttgctt ggaactggca
     1381 cctgctcagc agtcttttca tgtgccgcag gttcaggcac cgtttttgcc tcatctttag
     1441 gctcggccgc ctgtgcagca ttcttttccg cctgcgccat cgcgctattt ttcatttcca
     1501 tcagcttttt catgtctgcg acctgttttt ccagcgctgc agcacgagat tgttcttcct
     1561 tgatggcatt ttctttagca attgcgtctt cttgcgcagc agtcgccttt gctgctgctt
     1621 tatccgcctg ggtagtcgac ttttcatccc cagcagatag tttcaataca tcttttgcat
     1681 ctttggcttt tggaactggc ttttcacttg cactaccgat cttgccagca gactgttgtg
     1741 tattgcctcc agcattgctt ggtgcgctct ctttaacgac ttttgccaat gaattcttgt
     1801 aagcttgcca gttgcttgta tgttctgcta ccaaactcct ggcttgtgga cgactgatac
     1861 tatccagggt tgcctggtca ggcattcgaa taatcttgcc gactttgagg cggttcatgt
     1921 tcttgccgtc aaatgcctgc tggttggatt ggtacaatgc agccagcatt tgctcagtac
     1981 tgacattgtc aggcttcatc tgcctggcaa tcgcgtataa ggtatctccg cgctcagtgg
     2041 tcaatgcatt gactggtgtt tcagaggatt catcctgtgt tgctttagga gccggttttt
     2101 cggctacaga ttttgccggt tttttgggtg cggtaaccac tggttggctt ggctcattgc
     2161 tcattgcagc agccgacgtg ttttcagtgg tagttggcgc ggtttggctt gtcacaggca
     2221 aaccagcgga ctcagacaca tagttagaat taaagcctgg tggatccagt aaaagcgtgt
     2281 actctttgac cagtcgacca gttggccaat cgacctggat caacatatcc aggaaggctt
     2341 ctgtaatcgg ctgtgtgctg ctcaacttga ggataggctg gcctttgcta ttattgccaa
     2401 cttcaatctt gataaatggg taagaagcag gtttctccag catctggtct tgatagacct
     2461 gctcgctggc taacgccgcc tgaatactgc tgagttcccc tggcgtcact gccaatagct
     2521 caatttccgc attgagcggc tccccgagtt tagagctgga aatcatgctt cccaaaccgg
     2581 ctgcatgcac cgtcagggct gtactcaaac tcaaggcaaa ggctattttt tttaattgaa
     2641 atttactcac cgattaaccc ttagtaattc atggcgtttg tcaaaatagc atcattcaca
     2701 cagccacgca agcgcgccca ggcggcttga ggccaataaa tacatggatt tgaggctatc
     2761 gtccctatta aacaggatta tactaaaaac aaaagcccga aaagaccatg atttcattaa
     2821 taaatcatgg ctttacgggc tcctatttaa acaattactt aagcttcgac cagaattctc
     2881 aacatcctgc gtagaggttc ggcagccccc cacaacaact gatcacccac ggtgaaggct
     2941 gacaggtatt cgccccccat cgccagtttt cgtaaacgcc cgacgggtgt cgtcaaagta
     3001 ccggtaattg cagcaggtgt cagttcacgc atgctgattt cacgttcgtt tggaatcacc
     3061 ttggcccagt cattcgcctc tgtaagcatt tggttgattt cgtccaaagg cacattttta
     3121 cgcagcttga tggtcagtgc ctgagagtgg caacgcatgg caccaatacg cacacacagg
     3181 ccgtcaatga caatcgggtt tgcttctcga cctaaaatct tgttggtttc gacgccgccc
     3241 ttccattctt ccttgctttg gccattgccc aagtctttat caatccaggg aatcaggctg
     3301 ccagccaatg gcacaccaaa atttgactga ggcaaatctt cgctacgaat cgtatctgcg
     3361 actgtcttat caatctgtaa aatggctgaa gccgggtccg ccagtaaatc agcgactgaa
     3421 gcgttaatca cacccatctg gctaatcagt tcacgcatat tctgtgcgcc tgcgcctgaa
     3481 gcggcctgat aggtcatgga agttgcccaa tccactaaat ctgctttaaa caagccattg
     3541 agtgccatta acattaaaga cacggtacag ttaccgccaa tccagttttt gccaccatcc
     3601 accaatgcat ctttaatcac atgcatgttc actggatcta aaataatcac agcgtctttt
     3661 tccatgcgca gagtagaggc cgcatcaatc cagtggccgt tccagccagt cgcgcgcaat
     3721 tgagggaata tttcactggt gtaatcgcca ccttggcagg tcacaatcac atccatctga
     3781 cgcaaggcat caacactctt ggcatccttt agcgcaggcg actccttgcc taccttaggc
     3841 gcagccccgc ctgtttgtga ggtggtaaaa aattgcggtt caatatccgc aaaatcattt
     3901 tcctgcatca tgcgctgcat cagcacagaa ccaaccatgc cacgccagcc tacaaaccct
     3961 acttttaaca tagtgactcg atatttcagc cggattaccc ggcattacaa attagattat
     4021 ttggacgatt tctgaacagc ttcacccgct tgctcaatat ctttgcccat gccattaatg
     4081 gtattgcaac ccgtcaatac caacactgct aacaccaacg tcatccactt cgtcatattt
     4141 ttctcccaat gactaatctc taaaattgcc gaacttcaaa ggaaagtcgg taatactctt
     4201 tttaacgaat gcaatcgcct cttgcaagac atcccgcttg gcaccggtca ctcgcaccgt
     4261 atcaccctga atactggcct gcactttcat accactgtct ttaatcagct tggtaatttt
     4321 cttagctagg tcactatcga tgccatcctg cacccgtaaa tcctgtttca ccttgttacc
     4381 cgaaactttt tgtacatctt tgtgttccag gcgtttggaa gattcaggct cttttttctc
     4441 catggctggc aacaaaatgt cttttacctg gtccagctgg aagtcattgt caccaaacaa
     4501 ggtgatcact ttgtcttttt cgttgaactc aaccttggcc gacgtgcctt tgaagtcata
     4561 acgattggta atctgcttca cagacgcatc aatcgagttc ttcaaattga ccatatcgac
     4621 ttcggatgta atatcaaagc ttggcatatt taaacctcac aagcgatggc agaacaaggc
     4681 gtgaatgcgc aggcagtacg ctaagtacga caagcattca caacgcagtt ctgccaagca
     4741 gtcacaatta ttcgaagtgg cagacgtagt ctactgtttc aatcacctct atatcaaaac
     4801 tggaattacc cggcactgtg aatttctcgc cacctttata ggtcgtccag tgttcttcgt
     4861 ctttttgacg gactttgcag acaccgtcat taatttccat caactctgga gagccagtgt
     4921 taaaggtcaa tgtgctaggg aaaatcacac ccacagtgca gcgtgtaccg ttgggaaaca
     4981 acacggtgtg gctcacgcat ttgccatcaa aataaacgtt ggctttcttt tttacactga
     5041 cgttatcaaa ttgcgccatt tttaaattcc taatcaatac tagttacgac tatatcttaa
     5101 tatcaagcga acggcatact tagccgaccg ctgcgtagcc agttttacat ggccgctacg
     5161 atttggtcac ccatttcaga gcaagaaact ttcttcgtgc cctcttcata aatgtcgccg
     5221 gttcgataac ctgccgccaa tacctttttc actgcatttt caatgcgtgt ggcagccgct
     5281 tcgttatcaa agctgtaacg taacatcatg gctgcagaca agatggtggc aatcgggtta
     5341 gccaggtttt taccggcgat gtcaggcgct gagccgtgtg atggctcgta aaggcctttt
     5401 ttgctttcat tcagtgaggc tgaagccagc ataccgatag aaccagtcaa catagaagcc
     5461 tcatcagaca ggatgtcacc aaaaatattg ccagtcacca tgacatcaaa ctgtttggga
     5521 ttgcggatca gctgcatggc ggcattgtcc acgtacatat ggctcagctc cacttccggg
     5581 tattcttttg ccacatcgat gacaatttct ttccagaatt cagtggtttc cagaacgttc
     5641 gctttgtcga cagaacacaa tttcttgccg cgtttcatgg caatatcaaa cgccacgtgt
     5701 gcaatgcggc gtacttctgg ctcgctgtaa atcatggtgt taaagccttc gcgtacgcct
     5761 tgctcgttgg tacgcatccc gcgtggctgg ccaaaataca cgtcaccggt cagttcgcgc
     5821 acaatcatga tatccagacc agccactact tctggcttca gtgtagaggc attcgccagc
     5881 tctgggaaca ataccgcagg acgcaaattg gcaaacaagc ccaagtcctt acggatggcc
     5941 agcagaccac gttcagggcg caacgggcgg tccagggtgt cgtactgagg gccaccgact
     6001 gcgcccagca ataccgcgtc cgctttgcga cagatttcct gtgtgaactc tgggtatgga
     6061 tgaccgaact ggtcataagc ctggccgccc agcggcgctt cggtgaaggt catgtccaga
     6121 ccttcttttt tcaagacttc caggacgcgc aacgcttgcg caataatttc cgggccgatg
     6181 ccatcaccag gtaacactgc aatatttaat gccattattt ttccttaaac ttaatattct
     6241 tttgtcattc ctgaccaggt gagccaattt aagcttgtct ggattcccgc ctgcgcggga
     6301 atgacggatc aagcaaataa ccagggttgc gacgctttat gcttctcttc aaacgctttg
     6361 attttgtctg catgttgcag cgtcaggcca atttcatcca ggccgttcag caggttgtgc
     6421 ttgcgggttg gcgtaatatc aaagctgaat gactggccag aggacgtagt cacgatttgt
     6481 gcttccaaat ccacattcag cgtgtagccc tcattggcga agcattcttt aaacaggtta
     6541 tcgacttgct cagcagaagc cacaatcggc aaaataccgt ttttgtagca gttattaaag
     6601 aaaatatccg caaagctagg tgcaatgatc acacggaaac cctgatcttc aatcgcccaa
     6661 ggcgcatgct cacgtgaaga gccacaaccg aagttgtcgc gggccagcag tacttgcgca
     6721 ccctggtaac gtggctggtt gaggacaaaa tccggattca atggacgggt actgcaatcc
     6781 atgccaggct cgccatagtt ggtataacgc cattcatcaa acaggtaagg accaaaacca
     6841 gaacgtttga ttgacttcaa aaactgtttc ggaataatcg cgtcggtatc cacattggca
     6901 cggtccagtg gacacacgag gccatttaaa gtagtaaatg ctttcataat cttctcctct
     6961 tatgcgctgc gcacatccac aaagtggcct gcaatcgccg cagcagcagc catttccggg
     7021 ctgaccaggt gcgtacggcc acccgggccc tgacggcctt caaagttacg gttagaggtc
     7081 gaggcacaac gctcgcctgg gctcaatcgg tctgagttca tggcgaggca catggagcag
     7141 cctggctcac gccattcaaa accggcttca ataaagattt tatccaggcc ttcagcctct
     7201 gcctggcgct tcacctgccc agaaccgggc accaccatgg cgagcttgat attgccagcc
     7261 actttcttgc ctttggccac actggccgcc gcgcgcaaat cctcaatacg ggagttggtg
     7321 caagagccga taaagatttt atccagttga atctggtcaa tgggcgtatt cgcggtcagc
     7381 cccatatatt tgagggcgtt ctcaatactg gtacgtttgg tcgcatcggc ttcctgcgcc
     7441 ggatccggca cagtagaacc aataggcaat acttgctcag gcgaagtgcc ccaagtcact
     7501 tgtggcgcga tctcggcgcc gttcaatacc accactgtat caaatgtggc gtcgacatca
     7561 gagcttagcg ttttccagta agccactgct ttagtccaat tgtcaccttt aggcgcatgc
     7621 aaacggccct caagataagc agccgttttt tcatctggcg ccaccaggcc agcacgggca
     7681 cctgcttcaa tcgccatgtt acacagcgtc atgcggcctt ccatggacaa gccacggatt
     7741 acttcgccgc caaattcgat ggcgtagcct gtgccgccag cggtaccgat tttaccgatg
     7801 atggccagtg ccacgtcttt tgcggtaacg cctttgccta attggccatc cacacgcacc
     7861 agcatgcttt ttgatttttt agccaccagc gtttgtgtcg ccaacacgtg ctcaacctct
     7921 gaggtaccaa taccgtgggc taatgcgcca aatgcaccat gagttgaggt atggctgtca
     7981 ccacagacca ctgtcatacc tggcaaggtg gtgccattct caggcccgat gacatggata
     8041 atgccctggt ctttgttttt gaacgggaaa tacaccaaag caccaaattc tttaatgtta
     8101 ttgtccagcg tttccacctg taggcgtgag atcgggtctt caatgccttt atcccaatct
     8161 ttggttggcg tattatggtc tgggttggcc acaatgctgg aattacgcca tggcttgcgg
     8221 ccagccatgc gcagcccttc aaacgcctgc gggctggtga cttcgtgcac caggtgacga
     8281 tcgatataaa tcaaacaggt gccgtcagta tcctcacgca ccacatgcga ttcccacaat
     8341 ttgtcgtaca gcgttttgcc tgccatgaca gttcctttct caccacgcaa atatttataa
     8401 aactcgcgca gaatacctaa gtttcaatac ccttacaaga agatagttta tactagtatt
     8461 aacactaata ggataaacaa atcaaatgga tacaagacga caggaactgg cagaatttct
     8521 acaagccatg cgccaacgtg gcacccctga agaatttgga ttcccctctg gttcacgtcg
     8581 tcgaacgcaa ggcttgcgcc gcgaagaggt cgcccaactc gctggcatca gtgcaacttg
     8641 gtacacgtgg atagaacaag cacgtgaagt gaatgtatcg gctgaagcgc tagacagatt
     8701 ggcaactgct ctaaagctca gcaaatcaga acgcagctat ttatttgaca tggcagaccg
     8761 gcgtgacccg caggcccatc aaagtgaagt agatacagca ccggaaacac tggtcacgat
     8821 gctgtcacaa atccaaatac ccgcctatgt catggggcgc acatgggata tcctggcctg
     8881 gaatacgcct gcaggtcatt tatttaccgg tttgctcgat atcgcctggc cggaatcgca
     8941 acgccctaac ctactgcgct ttgtatttgc gaacccgcaa gccaggcaat ttgtagtgaa
     9001 ttgggaaatg cgggcacgta gactggtcgc tgaattcagg gcagactgcc gttcccgact
     9061 cgaagagcct gagg
//
LOCUS       DATMSK010000267         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_138663,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000267 DATMSK010000000
VERSION     DATMSK010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138663"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..344
                     /locus_tag="VNZ84_08840"
     CDS             27..344
                     /locus_tag="VNZ84_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWU67104.1"
                     /translation="MNIRPLYDRVIVKRVEQQRTTASGIVIPDTAAEKPEQGEVIAVG
                     GGKQLQDGSLRPLEVKVGNHILFGKYAGQTVKLNGEELLVMREEDILGVIESSANDLK
                     KVA"
     gene            380..1999
                     /gene="groL"
                     /locus_tag="VNZ84_08845"
     CDS             380..1999
                     /gene="groL"
                     /locus_tag="VNZ84_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443016.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWU67105.1"
                     /translation="MAAKDVKFHDHARTKIVKGVNILADAVKVTLGPKGRNVVLERSF
                     GAPVITKDGVSVAKEIELQDKLENMGAQMVKQVASKTADVAGDGTTTATVLAQAIVQE
                     GMKSVASGMNPTDLKRGIDKAVTALVDELKSMSKAITTHKEIAQVGAISANSDHAIGQ
                     IIADAMEKVGKEGVITVEEGKSMQNELDVVEGMQFDRGYISPYFINNPDKQVAALDEP
                     MILLYDKKISNIRDLLPTLENVAKANKPLLIIAEDVEGEALATLVVNSMRGILKVIAV
                     KAPGFGDRRKAMLEDIAVLTGATVVSEETGMQLEKVTLEHLGHAKRVEVQKENTIIID
                     GAGDAAKIKARVQSIRTQIEEATSEYDKEKLQERVAKLAGGVAVIKIGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALLRARSRISALKGENDDQEAGIRIVLRAIEEP
                     LRAIVKNAGEEPSVVIAKVLEATGNIGYNAATGEYVDMVETGVVDPTKVTRTALQNAA
                     SIAGLILTTDATVAELPKEEKKASAVPEMDY"
     gene            complement(2125..2964)
                     /gene="serB"
                     /locus_tag="VNZ84_08850"
     CDS             complement(2125..2964)
                     /gene="serB"
                     /locus_tag="VNZ84_08850"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HWU67106.1"
                     /translation="MRLVVQGPAISMAHLSHIHGLVGGHTQFMQVSQHAYYLPVAEGD
                     YIDVKTFCASQQIDCALVQDAHRLQKMGLCVMDMDSTLIAIECIDEIADMMNLKPQVA
                     EITEAAMRGELDFAASLRKRVALLKGLPETALQRVIDERLSFNPGAQAWINACKQHGI
                     KTMVVSGGFTFFANYVKDTLGLDYAVSNTLEIVDGKLTGNIVGDIVDAQRKADELIRL
                     RDQLGLSSSQTIAIGDGANDLKMMAVAEVGVAYHAKSVVQEQAMYALNVVGLDGITNL
                     LSV"
BASE COUNT          864 a          779 c          815 g          670 t
ORIGIN      
        1 aacttattcg ctaaggagaa ttgaacatga atattcgtcc cctgtatgac cgcgttattg
       61 tcaagcgagt cgaacaacaa cggacgaccg cttccggcat cgtaatccct gataccgccg
      121 cagaaaaacc cgagcaggga gaagtgattg ctgtgggcgg cggcaaacag ctgcaagatg
      181 gcagtttgag accattggaa gtgaaagtgg gcaaccatat cctgtttggc aaatacgctg
      241 gccagaccgt caaactgaat ggcgaggagc tgttggtcat gcgcgaggaa gacattctcg
      301 gcgtgattga atccagcgct aatgatctca agaaagtcgc ttaatcactg cactgctaat
      361 acagaaagga gaataaacga tggcagcaaa agatgtgaaa tttcatgacc atgcccgcac
      421 taaaattgtc aaaggagtga atattctggc cgatgcggtc aaggttacct tggggccaaa
      481 agggcgtaat gtcgtgctgg agcgcagttt tggcgcacca gtgattacca aagatggcgt
      541 ctccgtagcc aaggaaattg aactccagga caaactggaa aacatgggcg cacaaatggt
      601 caaacaagtc gcctccaaaa cggctgacgt ggcgggtgac ggtacgacta ccgccaccgt
      661 gctggcccag gccattgtgc aggaaggtat gaaatcggtt gcctccggca tgaaccccac
      721 agacttgaag cgtgggattg ataaagccgt gacggcgctg gtggatgagc ttaaatccat
      781 gtccaaagcc atcaccaccc ataaagaaat tgcccaggtc ggtgcgattt ctgccaactc
      841 tgaccatgcc attggccaga tcatcgccga tgccatggaa aaagtgggca aggaaggcgt
      901 gattaccgtg gaagaaggca agtctatgca aaatgagctg gatgtggtcg aaggcatgca
      961 gtttgaccgt ggttatatca gcccctattt catcaacaat cctgacaagc aggtggcggc
     1021 acttgatgag cccatgattc tgctttacga caaaaaaatc agcaatatcc gtgacttgtt
     1081 gcccacattg gaaaatgtcg ccaaagccaa caaaccgttg ctgatcattg ccgaagatgt
     1141 ggaaggtgaa gccctggcaa ccctggtggt caacagcatg cgcggcatcc tgaaagtgat
     1201 tgctgtaaaa gcgcctggct ttggtgaccg ccgtaaagcc atgttggagg atattgccgt
     1261 cctgaccggg gccactgtgg tttccgaaga aaccggcatg caacttgaaa aagtgaccct
     1321 tgaacacctg ggccacgcta aacgcgtcga agtgcaaaaa gagaatacca tcatcattga
     1381 tggcgctggc gatgcagcca agattaaagc ccgcgtgcag tccattcgca ctcaaatcga
     1441 ggaagccacc tcagagtatg acaaggaaaa actgcaagag cgcgtggcca aactggcggg
     1501 cggcgtcgca gtgattaaaa tcggtgctgc caccgaagtc gaaatgaaag agaaaaaaga
     1561 ccgcgtggat gatgccctgc acgccacccg cgcagcggtg gaagaaggta tcgtcccagg
     1621 cggcggtgtg gctctgctgc gtgcccgcag ccgcattagc gcacttaaag gcgagaacga
     1681 tgaccaggaa gcgggtatcc gtattgtgtt gcgtgcaata gaagagccct tacgcgcgat
     1741 tgtgaaaaat gccggtgaag agccttctgt cgtcatcgcc aaagtgcttg aagcaactgg
     1801 caatatcggc tacaacgccg caaccggtga atatgtggat atggtagaaa ccggtgtggt
     1861 agacccgacc aaggtgaccc gcactgcgct gcaaaatgca gcctcgattg ccgggctgat
     1921 tttgactacc gacgccaccg tggctgagtt gcctaaagag gagaaaaagg cctcggctgt
     1981 gcctgagatg gattactaat tcggtaacaa ccagtatcta tatttcaagc cccgcaaggg
     2041 gcttttttta tagctctacc attgtcaaac aggatgctag atgccagacg acactacgtc
     2101 agaacgctca ttcatggcgc aagcctagac agaaagcaag tttgttattc catccaggcc
     2161 gacgacatta agcgcataca tggcctgttc ctgcaccaca gatttggcgt gataagccac
     2221 gcccacctca gccacggcca tcattttgag gtcattggca ccgtcaccaa tcgcgatggt
     2281 ttgtgaggat gacagaccca actgatcgcg taatctgatc agttcatctg ccttacgctg
     2341 ggcatcgaca atgtcaccca cgatattacc ggtcagtttg ccatcgacga tttccagcgt
     2401 attggagaca gcgtaatcca gccccagtgt gtctttgaca taattggcaa aaaaggtgaa
     2461 gccaccagac accaccatgg ttttgatccc gtgctgtttg caggcattga tccacgcctg
     2521 cgcaccggga ttaaagctta aacgctcgtc aatgacacgt tgaagtgcag tttcaggcaa
     2581 gcctttaagc agggcaaccc gtttgcgcaa gctggcggca aagtctaatt caccgcgcat
     2641 ggccgcttct gtaatttcag ccacttgcgg cttgaggttc atcatgtctg caatttcatc
     2701 aatgcactct atcgcaatca gggtggagtc catgtccatg acgcacaaac ccattttctg
     2761 caatcgatgt gcatcttgta ccaaggcgca atctatctgc tggctggcac aaaaagtttt
     2821 aacgtcgatg tagtcaccct ctgccaccgg caagtagtaa gcatgttggg acacttgcat
     2881 aaactgcgtg tgtccgccaa ccaagccatg gatatgagac aaatgcgcca tgctgatggc
     2941 aggaccttga accactaaac gcatgataaa aatcactaat taaacaaaag cgcgattata
     3001 ccagttgcct ccccaaacgc tgagtaaagg ctggttttaa ggagtatttc cgtttttgga
     3061 ttagcgcaag caatgatatg ctttcatgca aattaaaaac ccccgggaaa agtaatgaaa
     3121 caagacgc
//
LOCUS       DATMSK010000268         8233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_210331,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000268 DATMSK010000000
VERSION     DATMSK010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210331"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VNZ84_08855"
     CDS             <1..905
                     /locus_tag="VNZ84_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67107.1"
                     /translation="MIQSLIEQCPILLKGQWQVNSLPPLKTLWQQLGIENADVAAILC
                     ARRSWPSTRPTFCLLPVHLGLRRDTFSLQSVVTLTADEYAALTERLKNHFSDDLVVHT
                     DESQRYWWIRPLRDMDAYARWPQDCLYQQAFQWQPQGADASVIRQWTNEMQMLLHDMT
                     YAPAGGHGPDVLNSLWFASVGDLPQSPSSAAIIGGQGAIFSGLLASQLPGVQSNSMAT
                     MWADKKITNAVWVADQWQSVDWADIADAIQTGQVSELNVVLPFAERSVHIQYKKQFRW
                     QFWRKAYTLETLLQQLETTLSTIR"
     gene            938..2623
                     /gene="recJ"
                     /locus_tag="VNZ84_08860"
     CDS             938..2623
                     /gene="recJ"
                     /locus_tag="VNZ84_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HWU67108.1"
                     /translation="MKIQQRTYSNSNFETLLAQGLPPLLARLLAARAVSADDWVLQEM
                     PHLLKPDALTGATQMAQLLADAIAQQKRILIIGDYDADGATATSVAMRGLLMMGAQVD
                     FLVPNRFEYGYGLTPEIVALAATQQPDVILTVDNGIASIDGVIAAKALGMQVLITDHH
                     LPAEQTPAADCIVNPNQRGCDFPSKHLAGVGVMFYVLIALRAELKQRGMLAEDRVHLG
                     SLLDLVALGTVADLVKLDANNRILVRQGLKRIRQGQSCPGILAILKLAGRDPEQVNAQ
                     DLGFYVGPRLNAAGRLDDMTIGIRCLLAETPQEAMQYAQQLHVLNAERKQIEAEMQWD
                     AMADLSQDFKDTQYTISVFQPDWHQGVIGILASRIKERFHRPVIAFADAGDGLIKGSG
                     RSIAGLHLRDALDLVTKQQPDLIVKFGGHAMAAGLSIRATDFMRFQHTFEQVVQSLIN
                     AETLQEVLETDGGLEVHEMTMQTAELLTQQVWGQGFPQPVFYDRFQVVQQKVLGEKHL
                     KLRLQKHNQQFEAIYFRHAELLPEQIEIAYALQVNEFNGNRNLQLQVMHAVTA"
     gene            2699..3808
                     /locus_tag="VNZ84_08865"
     CDS             2699..3808
                     /locus_tag="VNZ84_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWU67109.1"
                     /translation="MARPFKSPKTVSGTRPQKEQIPLAETAEGVDAPSVPRSGQRLHK
                     LLALAGFGSRRDMETLIESGRITINGQVAQVGAVVDEHDIVRLDSRILHLPFEAELPQ
                     VLLYHKPEGELVSQDDPEGRATVFDKLPRIKQGKWIAIGRLDMNTSGLLIFTNSGELA
                     NHFMHPRYEVEREYAVRILGELTPEQMSQLTHGIELEDGPANFESIYANGGEGANKWY
                     QVVIKEGRNREVRRLFEAFHLPVSRLMRVRFGPIALPPRLKRGTMLKLEQKQVVALLE
                     WAGLPVPRKPLKQMSPHDRDRATRVFTPKVRKQRRSLLDAANTPAGTQQERPARAPSG
                     NRRIRQDSDLKQASLQNARINSGAKKRSDRNRGRG"
     gene            complement(3891..4793)
                     /gene="prmB"
                     /locus_tag="VNZ84_08870"
     CDS             complement(3891..4793)
                     /gene="prmB"
                     /locus_tag="VNZ84_08870"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWU67110.1"
                     /translation="MTTNLQTIRDWLRYAVSRFENSDIFYGHGTDNSYDEAVWLIMSA
                     LHLPHDTLNNFLDARLTTEESQHLATLIEKRVTDHIPTAYLVNEAWLRGFKFYVDERV
                     IVPRSFIAELLEDGLQPWVEFPEMVESAADICTGSGCLGVLLADAFPNAHIDVVDISP
                     DAIDVCNINIGRYGLEHQIQAVQSDMFTALKGRKYDVIISNPPYVDAPSMSELPEEYR
                     KEPQLALGSGVAGLDHTHTLLTQAAEYLNDGGILVVEIGHNRDALHEAYPDLPFTWLD
                     TSAGDQFVFMLTKEDLIEAGLQVQ"
     gene            complement(4795..5937)
                     /gene="dapE"
                     /locus_tag="VNZ84_08875"
     CDS             complement(4795..5937)
                     /gene="dapE"
                     /locus_tag="VNZ84_08875"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HWU67111.1"
                     /translation="MSKTLDLAIDLLKRRSLTPEDADCQETMIARLEPLGFKIERMRF
                     GQVDNFYARRGTAGPLIVFAGHTDVVPTGPLDQWHTPPFEPTIKDGMLYARGAADMKT
                     SLAAFITSIEEFIAEHPDHSGSIGLLITSDEEGIAIEGTVKVVEALQARGETFDYCIV
                     GEPTSNKLVGDMIKNGRRGSLSGKLTVKGIQGHIAYPHLVKNPIHLAAPAIKDMVETV
                     WDNGNEYFPPTSWQISNMNGGTGATNVVPGEVEILFNFRYCPEVDGQGSSEQSLRSRV
                     HAILDSHGFDYSLEWEHNQSYITPRGELVAAISQAIEQSYGVSPELSTTGGTSDGRFI
                     ADICKEVIEFGPLNATIHKLNECVAVADIEPLKETYKRTMELLLLK"
     gene            complement(5942..6295)
                     /locus_tag="VNZ84_08880"
     CDS             complement(5942..6295)
                     /locus_tag="VNZ84_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HWU67112.1"
                     /translation="MKLYGIPNCNTVKKARDWLEQHAVAYEFHDFKKLGLNTETAQSW
                     LQQQPWEKLVNRSGMTWRNLTDTEKNAVTDAASALRLMQEKTSVIKRPIVVKNDKIIA
                     LGFNEAEYAQLFAKE"
     gene            complement(6375..>8233)
                     /locus_tag="VNZ84_08885"
     CDS             complement(6375..>8233)
                     /locus_tag="VNZ84_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU67113.1"
                     /translation="PVDFIGSLLCAYLLSLSLLKTANQKKLPLRDLLYASLTAGVSGY
                     LLSYFFQISAGAINIHIDTWTALFSLFLACLTSALAIVTILWLKSYEGKALHRLKWMF
                     SVEIALGFLASHTAINLSIVHNNMLIGQPTDQAENYLVLLLVLVPVLLFITSFILVIF
                     YERNVDLTQSRLTFRNTQTVNKRFLAYDPLTHLPNRDALNQHLIISAKRCDRNGESLA
                     LAYIDLDHFKPVNDQFGHHIGDLLLIEVSKRISNAIRNCDYVARAGGDEFIAVLSEIE
                     NHQSVVTVIQRIIDALREVFIIEDHVIEISCSIGVSMYPQDGNLQKLKLNADAAMYKA
                     KENGKNQYRFFDAEIEQASDEMQQTRVELKKAITEQEFRLLFQPKVDSLTRLVHGAEA
                     LLRWQHPTRGLLSPNSFIEAAERFGMIEEINAWVISQACSTIKLARDRGIDLSISVNM
                     SNQQFRNKQLGSLIQSIMESHEVEARNLILEVSETNAIHHQAQFKETLRHFKNQGLKI
                     SLDDFGLHPFSLMHLQDLEISEVKLDRTLTKGVAASRTSLSIVEAIVKLAHALDLNVV
                     AEGVEDEDQRQALVSIGCDQMQGFLFSKPVEQKFLFDVFSELQLKSTTQSLF"
BASE COUNT         1995 a         1976 c         2206 g         2056 t
ORIGIN      
        1 aaatgattca gtcgctgatt gaacaatgcc caatcctttt aaaggggcaa tggcaagtaa
       61 actcattgcc gccattgaaa acattgtggc aacagctggg tattgagaat gccgatgtgg
      121 cggccatcct ctgtgcaagg cgaagctggc catccactcg ccctacattc tgcctgttac
      181 ctgtgcatct gggtcttcgg cgcgatacct tcagcctgca atcggtcgtt accctcaccg
      241 ctgacgaata tgctgcatta actgaacgtc taaaaaatca cttcagtgat gacttggtgg
      301 tacacacaga tgagagccag cgttattggt ggatccggcc tttgcgtgat atggacgcct
      361 atgccagatg gccacaggac tgcttgtatc aacaggcatt tcaatggcag ccgcaaggtg
      421 cagatgcctc tgtcatcagg cagtggacca atgaaatgca aatgctgtta catgatatga
      481 cctatgcgcc tgctggcggt catgggccgg atgtattgaa tagtttgtgg tttgctagtg
      541 ttggtgatct tccccaatcg ccatcttcag cggcaatcat cggcgggcag ggcgctattt
      601 tttccggctt gctagccagc caattgccgg gagtgcaatc taacagcatg gctaccatgt
      661 gggcagacaa gaaaataacg aatgcagtat gggtggcaga tcaatggcag agcgtcgatt
      721 gggccgatat tgctgatgcc atacaaactg ggcaagtttc ggaattgaat gtggtgctcc
      781 cttttgctga gcgcagcgtc cacatacagt ataaaaaaca atttcgctgg caattctggc
      841 gaaaagcata cacattggaa acgttgttac agcagttaga aacgacattg tccaccatca
      901 ggtaaatagt ctccgtgacg aggtccatgg acatcagatg aaaattcaac aaagaaccta
      961 ttcaaacagt aattttgaga ccctgttggc gcagggtttg ccacccttgc tggcacggct
     1021 gctggcagca agggcggtca gcgcggatga ctgggtctta caagagatgc ctcatttgct
     1081 caagccagat gcacttaccg gcgctaccca gatggcgcaa ttgctggcag atgcgattgc
     1141 acaacaaaag cgcatcctga tcattggcga ttatgatgcg gatggcgcta ccgcaaccag
     1201 cgttgccatg cgtggcttgc ttatgatggg tgcccaggtg gattttctag tccccaaccg
     1261 atttgaatat ggatacggcc tgacgccaga aatcgtcgcc ttggcagcga cacagcagcc
     1321 cgatgtgatt ttaactgtgg ataacggtat tgccagcatt gatggcgtga tagctgcaaa
     1381 agcactaggg atgcaagtgc tgattactga tcaccatttg cccgcagaac aaacacccgc
     1441 cgcggattgc atcgttaacc ccaatcagcg gggatgtgat ttccccagca aacatttagc
     1501 aggggtaggc gtcatgttct atgtactgat cgccttgcgt gccgagctta agcagcgtgg
     1561 catgctggca gaagaccggg tgcatttggg cagtttgctt gatttggtcg ctctgggtac
     1621 ggtggccgat ctggtcaagc tggatgccaa taaccgcatc ctggtcaggc aaggacttaa
     1681 acgcatcagg caaggacagt cttgtcctgg cattctagct attctcaaac tcgcagggcg
     1741 tgaccctgaa caagtcaatg cacaagacct agggttttac gtgggcccac gtctcaatgc
     1801 ggccggcagg ctggacgaca tgaccatagg catacgctgc ctgcttgctg aaacgccgca
     1861 agaggccatg caatatgcgc agcaattgca tgtgctgaat gcagagcgca aacagattga
     1921 agccgaaatg caatgggatg ccatggcaga cctctcacaa gactttaaag acacgcaata
     1981 cacgattagc gttttccagc cagactggca tcagggtgtg atcgggatcc tggcttctcg
     2041 catcaaggag cgcttccatc gtccggtcat tgcttttgcg gatgccggag atgggctcat
     2101 caaaggctct ggccgctcca ttgccggttt gcatctgcgc gatgccttgg acctcgttac
     2161 caagcaacag cctgatttga ttgtcaaatt cggagggcat gcgatggctg ctggcttgag
     2221 tatccgtgcg acggatttta tgcgctttca gcacactttt gagcaggtgg tacaaagcct
     2281 gattaatgca gaaaccttgc aggaagttct ggagaccgat ggcggactgg aggtgcatga
     2341 aatgaccatg caaacagcag aactattgac gcagcaagtc tggggccaag ggtttccgca
     2401 gccggtattt tatgaccgtt tccaggtagt gcagcaaaag gtcctgggtg aaaagcattt
     2461 gaagttgcgc ctgcaaaaac ataaccagca gtttgaagcc atttatttcc ggcatgcaga
     2521 gttgttgcca gagcagatag aaattgcata tgccttgcaa gtgaatgagt ttaatggcaa
     2581 ccgcaatttg cagttacaag tgatgcatgc ggtgaccgcc taagtggtgg cttgtaatta
     2641 gccttagaga gtctgcccta aatacggtaa aatacagcct tcattcaaga ggttaagcat
     2701 ggctcgccca tttaaatccc ccaaaactgt ctccgggaca cgtccccaaa aagagcaaat
     2761 accgctagcg gaaactgcgg aaggtgtaga cgcaccgtct gtgccacgtt cggggcaacg
     2821 tttacacaag ctgttggcgt tggcagggtt tggctcacgc cgcgatatgg aaacgctgat
     2881 tgaatcaggc cgtatcacga ttaatggcca ggttgcacag gttggcgcag tagtggatga
     2941 gcacgatatc gtacgccttg atagccgtat cctgcatttg ccgtttgaag cggagttgcc
     3001 gcaagtcttg ttatatcaca agccagaagg cgagttggtg agtcaggatg acccagaagg
     3061 gcgtgctacc gtatttgata agctgccacg catcaaacag ggcaagtgga tcgcgattgg
     3121 ccgtctggat atgaatacca gcggcttgct gatttttacc aactccggtg agctggctaa
     3181 ccactttatg cacccacgct atgaggtaga gcgcgagtat gcggtgcgta tcttgggaga
     3241 gctcacgccc gagcagatgt cacaattgac gcatggcatc gagcttgaag atggcccagc
     3301 caattttgag agcatttatg ccaatggtgg tgaaggtgcc aacaagtggt atcaggtcgt
     3361 gattaaagaa ggccgtaacc gcgaagtgcg ccgcttgttt gaggctttcc acttgccagt
     3421 cagccgtttg atgcgggtgc ggtttggccc gattgcgctg cctccgcgcc ttaagcgtgg
     3481 caccatgcta aagctggaac agaaacaagt cgtcgctttg ctggaatggg caggcttgcc
     3541 agtaccgcgc aaaccgctta agcaaatgag tccgcatgat cgtgaccgtg ccacccgcgt
     3601 gtttacgcct aaagtgcgca aacagcgccg tagcctcttg gatgcggcaa atactcctgc
     3661 tggcacgcaa caagagcgtc ctgcacgtgc gccttctggt aatcgccgta ttcgtcagga
     3721 ttcagatttg aaacaggctt cgctgcaaaa tgcacgcatc aacagcggtg ccaaaaaacg
     3781 tagtgaccgt aatcgtggtc ggggttaagc tgctggtgat aataaaaagc acttctgatg
     3841 gaagtgcttt ttttatgact ggcattgccg tgttttttcg gcttgctggt ttattgaacc
     3901 tgcaaaccag cctcaattaa atcctctttc gtcagcataa acacaaactg gtcgccagca
     3961 ctggtgtcca gccaggtgaa tggcaagtca ggataggctt cgtgtaaagc atcccggtta
     4021 tgaccgattt ccacgaccag gatgccgcca tcatttaagt attcggccgc ctgcgtcagt
     4081 aaagtatgcg tatgatccag tccggcaacg ccgctgccta acgccagttg aggttctttg
     4141 cggtattctt caggcagttc agacatgcta ggcgcatcca cgtaaggtgg gttgctgata
     4201 atcacatcgt atttacggcc cttaagcgcg gtaaacatat ctgattgtac ggcttgaatc
     4261 tggtgttcaa gtccgtagcg gccaatgttg atattgcaga cgtcgatggc gtcaggtgaa
     4321 atatctacga catcaatatg tgcattggga aaggcgtcgg ccaacaagac gcccaggcag
     4381 ccagaacccg tacaaatatc agccgctgat tccaccatct cggggaattc aacccaaggc
     4441 tgcaatccgt cttccagtaa ctctgcaata aatgagcgtg gcacaatcac gcgctcatcg
     4501 acatagaact tgaatccgcg taaccaggct tcattgacca ggtaggcggt cgggatgtgg
     4561 tcggtgacac gtttttcgat cagggtggcc agatgttgac tctcttctgt agtcaagcgt
     4621 gcatccagga agttattcag tgtgtcgtgc ggtaaatgta gcgcgctcat gatcagccaa
     4681 acggcctcgt catagctgtt atcggtaccg tgaccgtaaa aaatatcgga gttttcaaag
     4741 cggctgacgg cataacgcag ccagtcgcgg atagtctgta aattagtcgt catattattt
     4801 cagcaacaat aattccatgg tgcgtttata agtttccttc agtggctcaa tatctgccac
     4861 ggccacgcat tcgttcagtt tatggatcgt ggcattgagt ggaccgaact caatcacttc
     4921 tttgcagata tcggcaataa agcggccatc agaggtgcct ccagtggttg aaagctcggg
     4981 gcttacgcca taactttgct ctatggcctg gctaatggcg gcaaccagtt cgccacgtgg
     5041 ggtaatgtaa gactggttgt gttcccattc aaggctatag tcaaaaccat ggctatccag
     5101 gatggcatgt acgcgtgaac gcaggctttg ctcggagctg ccttggccgt ccacttccgg
     5161 acaatagcgg aagttgaaca ggatttcgac ttccccaggc acaacattgg ttgcaccggt
     5221 accgccattc atattggaaa tttgccacga agtaggcggg aaatattcat taccgttatc
     5281 ccacaccgtt tccaccatgt ctttgatggc gggcgcggcc aggtgaatcg ggtttttgac
     5341 caggtgcggg taggcgatat ggccctggat acccttgacc gtcagcttgc ccgatagtga
     5401 accacgtcgg ccattcttga tcatatcgcc taccagcttg ttactggtgg gttcgccgac
     5461 gatacagtaa tcaaaggttt ccccacgcgc ttgcagggct tcgacgactt taacggtgcc
     5521 ttcaatcgca ataccttctt catctgaagt gatcagcagg ccaatggagc cagaatggtc
     5581 tggatgctcg gcaataaact cttcgatgct ggtaataaaa gccgccagag acgttttcat
     5641 gtcggcggca ccacgcgcat agagcatgcc atctttaatc gttggctcaa agggcggggt
     5701 atgccattgg tccagtgggc cggtaggcac gacgtccgtg tgtccggcaa atacaatcaa
     5761 tgggccagcg gtgccccggc gtgcataaaa gttatccacc tggccaaagc gcatacgctc
     5821 aatcttgaat cccaagggtt ccagtcgcgc aatcatggtt tcctggcagt cggcatcttc
     5881 cggtgtcagt gagcggcgtt tgaggaggtc aatcgcaagg tccagtgttt ttgacataaa
     5941 cttattcttt ggcaaacagt tgggcgtact cggcttcatt aaaaccgagg gcaatgattt
     6001 tgtcgttttt gaccacaata gggcgtttaa taacagaggt tttttcttgc attaaacgca
     6061 acgccgaagc ggcatccgtg actgcatttt tttcggtatc tgtcaggttg cgccaggtca
     6121 taccactgcg attgaccagc ttttcccacg gttgctgttg cagccaggat tgtgccgttt
     6181 cggtgttcag gcccagcttt ttaaagtcgt ggaattcata ggcgactgca tgctgctcca
     6241 gccagtcacg ggctttcttg acggtgttgc aattagggat gccgtacagt ttcatggcgg
     6301 tctatcgggt aaaaaacctt attttaccag cccgtgccaa gggcgttgca atttattctc
     6361 gcgggtagct gactttagaa caggctttgg gttgttgatt tcagttgaag ctcgctgaat
     6421 acatcaaaca ggaatttttg ctctaccggt ttggagaaga gaaagccctg catctggtca
     6481 cagccaatgc tgaccaaggc ctgccgttgg tcttcgtcct ccacaccttc tgccaccaca
     6541 tttaaatcca gtgcatgtgc cagttttaca attgcttcta caattgaaag cgaagtacgt
     6601 gaagccgcga ctccctttgt cagggtgcgg tccagtttca cttcgctgat ttcgaggtct
     6661 tgcaagtgca tcaggctaaa aggatgtaag ccaaaatcat cgagggaaat tttcagtccc
     6721 tggttcttaa agtgacgcaa ggtctccttg aattgggcct ggtgatggat ggcattggtt
     6781 tcagagactt cgagaatcag gttgcgtgcc tcgacttcat gggattccat gatggactgg
     6841 atgagcgagc cgagttgctt gttacggaat tgctggttag acatgttgac cgagatgctc
     6901 aagtcaatac cgcggtcacg tgccagtttg atggtgctgc acgcttggga aatgacccag
     6961 gcattgattt cctcaatcat gccaaaacgt tcggcggctt caataaaact atttggcgac
     7021 agcaggccac gtgtaggatg ctgccagcgc aggagtgcct ctgcgccatg aaccagccgg
     7081 gtcagtgagt ctactttggg ctgaaatagt aatctgaact cttgttcggt aatcgctttt
     7141 ttgagctcca cacgtgtttg ctgcatttca tcggaagcct gctcgatttc cgcatcaaaa
     7201 aaacggtact gatttttgcc attttccttg gctttgtaca tcgcggcatc ggcgtttaat
     7261 ttgagctttt gcaggttgcc atcctgcggg tacatggaca cgccaataga acaggagatt
     7321 tcaatcacgt gatcttcgat gataaagact tcacggaggg catcaataat gcgctggatt
     7381 acagtgacca cgctttgatg attttcgatt tcgctgagta cggcgataaa ctcatcgccg
     7441 ccagcccgag ccacataatc acaattacgg atggcattgc tgatgcgctt ggagacttct
     7501 atcagtaaca ggtcgccaat gtggtggcca aactggtcat taacaggctt gaagtgatcc
     7561 agatcgatat aagccagggc taatgattcg ccattccggt cgcaacgttt ggcgctaata
     7621 atcaggtgct gattcaaggc atcgcggtta ggcaggtgcg tcagcggatc gtaagccagg
     7681 aatcttttgt tgactgtttg agtgttgcgg aaagtcagcc tgctttgggt aaggtctaca
     7741 ttccgttcat aaaagatgac caaaatgaat gaagtgataa atagtagtac cggtaccaat
     7801 accagcagta acacaagata gttttcggcc tggtcagttg gctggccgat cagcatgttg
     7861 ttgtgcacga tactcaggtt aatggctgtg tgggatgcca ggaaaccaag cgcgatctcg
     7921 accgaaaaca tccatttcaa gcggtgtagt gcttttcctt cgtagctttt taaccacagg
     7981 atggtgacga tagccaatgc cgatgtcaga caggcgagga aaagggagaa caatgcggtc
     8041 caggtgtcaa tgtggatatt aatggcaccg gcactgatct ggaaaaaata ggacagcagg
     8101 tatccgctga cgcccgcagt taatgaggca tagagcaggt cgcgtagagg cagttttttc
     8161 tggttagccg ttttaagtag cgatagggaa agcagatagg cacagagcag gctgccgata
     8221 aaatctaccg ggt
//
LOCUS       DATMSK010000269        26184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_92352,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000269 DATMSK010000000
VERSION     DATMSK010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 26184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26184
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92352"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="VNZ84_08890"
     CDS             <1..2010
                     /locus_tag="VNZ84_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWU67114.1"
                     /translation="GRVLCGLPPDPEYKGVHGYNQLKLKHPFDSREVPVIYGDHVTTD
                     AGTGLVHTAAAHGNDDWLVMRANFPNEKPRVLIGGDGKFFTSELVELADIRGLSRQDA
                     NKIILAKLQENGVLFASARLNHSFPHCWRHKTPLMQLATHQWFIGMNSVGKDGKALRD
                     LANMSVDATQFFPSWGRARLEAMIKNRPDWCVSRQRNWGVPMPFFVHKETGEPHPQTM
                     QLLETVRKQVEQQGIEAWFSLDGAAFLAQHAPANAEQYKKVTDTLDVWFDSGATHYAV
                     VRSARHPSLSAAANVREAEHQPVADLYLEGSDQHRGWFQSSLLTGCAIDGNAPYKALL
                     THGFVVDGAGHKMSKSKGNVVAPQKVMDTYGADILRLWVASTDYSGELTISDEILKRV
                     ADGYRRIRNTLRFLLSNLADFDASKDLLPVDQWLEIDRYALHLTQQLQTGILADYNKY
                     EFHLAVQKFVSFCSEDLGGFYLDILKDRLYTSGETSHARRAAQSALHHITHAMMRLMA
                     PILSFTADEIWQTLGLDNVATVFEEDWYGLPACGLSESVLADWENLRQFRDIANKKIE
                     EKRGEGVVGSSLQAELDIHVDGNLYDSLSRFGEDLKFALITSRANVYKQVGAGVSVDV
                     KSSANAKCDRCWHYRADVGRDAAHPTICGRCVSNLFGKGEVRTYA"
     gene            2003..2479
                     /gene="lspA"
                     /locus_tag="VNZ84_08895"
     CDS             2003..2479
                     /gene="lspA"
                     /locus_tag="VNZ84_08895"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWU67115.1"
                     /translation="MPKLRSALPWLLLSAIVIALDLYTKHLVQQAFVYGEHVPVTGFF
                     DLVRYHNTGAAFSFLANAGGWQHGFFTAVSTLASAVMVYLLVKHPEKRLFCFGLALVL
                     GGALGNLYDRLTLGYVVDFLLFYYQQWAWPAFNVADSAICVGVALLMLDSFKQPNT"
     gene            2520..2834
                     /locus_tag="VNZ84_08900"
     CDS             2520..2834
                     /locus_tag="VNZ84_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HWU67116.1"
                     /translation="MDGLSYRFNDVIKLWVGSKSTWHYVSLPQGMSDEIHTLSQYHQT
                     KRRGWGAVKVDVSVKDQQWQTSIFPAFDNQRYALFLKADVRKQLGVAVGSEVTVTLKI
                     LF"
     gene            complement(2812..5136)
                     /locus_tag="VNZ84_08905"
     CDS             complement(2812..5136)
                     /locus_tag="VNZ84_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU67117.1"
                     /translation="MTLSRQVIVVALSVSLVCLLATINATPETALWLDNIHWFSTITA
                     CLILATLGYLRANPSHRPCKRWFVIGALSYFTGGLLWIFEVLTDQTTFPAQSDLFYPL
                     MGPCLIIGFVVALRSQLSRAKTLAFTVDALSFSVAVIGYILISYLPQATAVSPLELTV
                     LTAYPASMLSAALVSLLLIPYLRPTCMKPWLMLSAGLSLLGICWMQWNLNALNRTPQT
                     SLWINYGFSAADLLIATGLYGWQVRITKSAVYHRQCRRLRSWIPVIAFTFSISTLMLI
                     WWQSGPHPYAYHIAIGSVTVILIFATIRQSILLQESERLLRAEKRLAEKELEYQQLSR
                     HDPLTHLPNRLAMLNLLEHAIIGAASESGMVALLMIDLKHFQSINDSFGHKTGDLFLI
                     ETAQRLQYIAHDCGELGRVHGDKFALIITRRKTDAELVHLAHNLCKIVHKPITIENHE
                     LILDACIGISLYPRDANNSEQLARNANTALAHAKRSSQHLFMFYSKEQTKLAQNRFKL
                     DVQLRKAIRRQEFFLQFQPIFCLDKNTQPRIAKIEALIRWKTHKGDIISPGEFIPYAE
                     QSGLILPLGEWVVAEAFRQLAALSSKSSQPVGLSINISPRQFRDHSLPLRIAQHLKQY
                     AIPPACITLEITESAVFEHEEQALEILKQLKALGVRISLDDFGVGNSSLFKLKQLPID
                     ELKIDRAFMVDVPESSADSEIVSTIIKTAGILGISVVVEGVETQAQLDFLRQVGCDCI
                     QGFLLGRPMPIEDIQELLATAQAEPMTPKKEFSM"
     gene            complement(5183..6079)
                     /locus_tag="VNZ84_08910"
     CDS             complement(5183..6079)
                     /locus_tag="VNZ84_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HWU67118.1"
                     /translation="MAASSLLALLDDITTMLDDVSVMSQLAAKKTAGVLGDDLALNAQ
                     QVTGIAAQRELPVVWAVARGSLINKLILVPAALLISAFLPWLITPLLMLGGAFLCFEG
                     FEKIWHKLRHIEKEDVHTQVLHAALQQPKTDLVALEKEKIKGAVRTDFILSAEIIVIA
                     LGVVAEAALTQRIVVLSLIAVLMTIGVYGIVALIVKLDDIGLWLMQRQGQGVLPQSTR
                     KFGSGLVQVVPSLMRLLSVLGTAAMFLVGGGILAHGIHPLHALVAQLPHWSLLINAAI
                     GVLMGAICFTVVELVKLFLRRD"
     gene            6178..6576
                     /locus_tag="VNZ84_08915"
     CDS             6178..6576
                     /locus_tag="VNZ84_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HWU67119.1"
                     /translation="MALMAIMLATLMPTLTVAFPMQSGKTFVQEVCSSQGNKLVIQVL
                     TTQGKLRATLIDYQPSQKPTSLSHHLNHCPFCQMALDQATIPSHNPSYILFQKAQAQI
                     ALSEYQAPVVSSFYPTAHLSRAPPLTPHSV"
     gene            6640..6888
                     /locus_tag="VNZ84_08920"
     CDS             6640..6888
                     /locus_tag="VNZ84_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU67120.1"
                     /translation="MIIINLQAMIFAKQVEWKRHITLKEVADATGISRMTLHRMVKNP
                     TYNACTEHLGKLCTYFDCDISALIRWQPNTAVRQIIAA"
     gene            6953..9013
                     /locus_tag="VNZ84_08925"
     CDS             6953..9013
                     /locus_tag="VNZ84_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWU67121.1"
                     /translation="MNKTKIGLAILSALPYLAHAEHEGTVHLDEVKVVAKKINSSTTQ
                     PDINTAKENLSKTAGGTTVVDMATVREGRTSNMQDTLGMATGVLAQSRFGAEETRLSI
                     RGSGLQRTFHGRGLKLMQDGIPTNLADGGFDFPAIDPMATDYIEVYRGANALQYGSSN
                     LGGSINFISRTGYTAPALEVKTEGGSYGYYRLGVSTGGVKENVDYFVTASKYHTDSFR
                     DNAQQGADRLTGNVGVKINDQVETRFYFGYVNNDSQLSSSLTKAQLKDNPKQATVIPG
                     QGINERNINVTRLANKTTILFDSSKLELGAFYAHKSLFHPIGNFGDTTGVIDQVSNDI
                     GLTARWSHQGELFGMSNEFVAGLSPTSGRIDAKNYVNVNARRGALLNDFDQKATNFES
                     YLEDRLSLDDQWTVIAGLQYAKSKRDSKDQLITASGDQSVNKTYSQTSPKLGVLYQLQ
                     PQVQVFANASRSFEPPSFGELNSLVVSSLKAQKGTTFEIGTRGNSEFVDWDIALYDAR
                     LQDELLAVAAAGSPVATQTVNAGRTIHRGLEFGLTARLPYSLQWRSSLLVNDFKLDND
                     PTYGSNRLPGIQRSLLRAELVYRGSDVLENFYIGPTIEWSPQRYNVDFAETLYADSYF
                     LWGMKAGQKVNQHWSWFVEGRNLANQKYAATTGVARNQNGADGALFLPGDGRTAYLGV
                     TWTY"
     gene            9058..9543
                     /locus_tag="VNZ84_08930"
     CDS             9058..9543
                     /locus_tag="VNZ84_08930"
                     /inference="COORDINATES: protein motif:HMM:NF033672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper uptake system-associated protein"
                     /protein_id="HWU67122.1"
                     /translation="MKLILKFSLLFIALSIAPAYGHSTAAADDEQSVAKEMHETWDKP
                     NQPLVLPVIVIHHGMAIADWIQGDKGGRALLKYHPEHQHWHTLLCSGAELTAPRHLQE
                     AGMSAADAQSLALLLQQQEQALPDDQRQQIDSFSGVVKFSKGKPVSSVGHEMHGEAHG
                     H"
     gene            9533..11014
                     /locus_tag="VNZ84_08935"
     CDS             9533..11014
                     /locus_tag="VNZ84_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWU67123.1"
                     /translation="MAIKISTVKNQWLFWHQWIGIVACIGMVLWGLSGASHPLMSRLQ
                     PVPKQFMPPLVPVDMSQAVALPGLLKQQGITQFQHISLVQLEGQGYYRIAQAGAVRYF
                     AMADGAERADAEQALAVNLANYYTGRNDPSIWAVKPVTQFEPDYHPVNRLLPVWRVAY
                     DGDEGLRAYVDTEQSRLATLVDDRRAFLTKVFQFGHNWSFLQACSTLQLAVATLVLVL
                     ILASAVTGVVLYIKQRKTAPFRLKRWSLQWWHREFGVWVSLVILLLASTGLFHLWMSD
                     MQQRRPGMPTVYASESVALSEAAWAKVATGQVLKLDIYGRGATLFWQVIPVVAGEQAL
                     PSAQVAAMHHHDHAGHAQRPAEPVLWLTRSQETTVLTPQAYASAWLAVMAGNPSPAIQ
                     SVAWVTSFANEYGFVFKRLPVLKVQTAQTEHTRYYLEPATGMLAATINDTDGLEGFVF
                     AYLHKWSFQSVSKDVRDLLAVIAALAVSLTGLLGAYLFAKKRM"
     gene            complement(11054..12337)
                     /locus_tag="VNZ84_08940"
     CDS             complement(11054..12337)
                     /locus_tag="VNZ84_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWU67124.1"
                     /translation="MPNHASDTYYIDIDQLQVGLYIHLDLGWMDHPFSVSNFKITDES
                     QIETIRSLGLKQLRYDPRRSSSTPLRAAAYQNVVEFPLRKATSPRLEIIDDAPELSPA
                     QKKQLALRQAIAESEQKFMKTSSEVKNIQKLSTERPEQAYGLASRLVEDLVSSTLTEG
                     DVAIHAMNGHRIGDQHFQHELNTLVVAMLLAKNLDITDDEAVVLGMAAILHDIGKRQI
                     SDKILLKKDPLSVRETSIYQTHVLLGLNMLRDVAVPRRILTLISQHHEFADGSGYPKG
                     LHCDQIDPLSHILIIANMYDNLCNPSNPALTKTPYEALSVMFAQQRHQFDQSILRRFI
                     KCLGIYPPGSVVRLSDQKLATVLSTNPQQPLRPFIQMLSDDREEEGEIIDLREALDIN
                     IIQCLKPEQLPNALQSMLRLKQRTSYFLDKIYAAS"
     gene            complement(12330..15272)
                     /locus_tag="VNZ84_08945"
     CDS             complement(12330..15272)
                     /locus_tag="VNZ84_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU67125.1"
                     /translation="MSLPTVFDHHSTAWLQPLVATMPDTTMLALNRRAQCVAASARAQ
                     ALLGDHAESLPKEVRQALVQAMREGQIELNIHLSVHGLKREFKCRLFYPPSDRRAVGL
                     MLEETQRTLTDQLRHMQSIVHSLIEMSPDLICIKDHENRWMMANQHMLNTFQMDGFDY
                     EFLNNLQIADTLHPVFRNSFEHHEKSDQLVWQTRQAYHNEELVHLPQGGHKTLAVNKI
                     AYHDQKDQPSHLITVARDVSEQKFIDTQLQNRSAVLDTLISCDWMLHSAERWQSVAEN
                     VLQQCCLSFRFSRAVLLQHETISTHKKTEIRSKAMFHWAINGFHNTYLHWEKISFLSP
                     ELQRWYTLLSKGQPVMCDALTLPETERALLKSHDTVNLVIVPLMIDDEWWGSMVIERC
                     FDAENTSSQELGSLMAIGRTLSVAIDRERTGKHLKLAKIAFDSTTEGIMIVNPAGELV
                     GINQGYTLITGFEESEVLGTKPQIFRQRPRGLFGALKRHGKWSGEIENMRKNGDSYTE
                     WMTITAVRNHANVITNYVGVFADISEMKRSQTQLNSLVNHDPLTGLPNRRLINHLFTH
                     AIQRAHRNGHQAAVLFIDLDRFKNINDSLGHYSGDQLLLGVTERLRASLRESDIVGRL
                     GGDEFIVLLENLHSKADATQKAHDILDSLRRAFMIDHHELFIGASIGISMYPHDGQDV
                     EGLIKAADLAMYQVKNNGKNHVAFYDAALSRDAVEHFQLETELRQAITKNQLKMFYQA
                     QIDLKTGMIIGAEALIRWQHPTQGMISPVKFIPLAEETGLILSIGEWALTQAVRQAHT
                     WHKKHPEFKKIAINVSGVQIMKSKFADTVYGILMDSECDPSIIEVELTESTLMQHTQH
                     VVDTFNQLKLLGVSIAIDDFGTGYSSLSHLKRLPLDQLKIDRSFVKDLPDDKDDAAIT
                     SAIYAMATQLGLSVVAEGVETEEQEKFLQRLGCQTAQGYLYAYPVNAEDFLQLLVLNN
                     QRKIKRHA"
     gene            15412..16596
                     /locus_tag="VNZ84_08950"
     CDS             15412..16596
                     /locus_tag="VNZ84_08950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWU67126.1"
                     /translation="MKQPISQWLLASVLCMLPLAQAHENHAEHTGHPLAVSVVADEAG
                     HVLVAQVKQGQVMVAVSEDAGQHFNQGVAVNATPMQVAADAEARPHIAVGPDGNVYVT
                     WTESLRKSYAGNIWFARSVDGGKSFEPPYIVHQHREEITHRFDVLQVTANGSITVAWI
                     DKRDALAAKAAGKFYEGAAIYYAVSNDHGKSFHPEQKLADSSCECCRIAMTGKADGTV
                     AVLWRHVFDDGERDHAMAEISPGARPVLVRASYGHWKLDGCPHHGAALAVGEGFGYHL
                     AYFDGAGNRPGLRVARMDDHAWVTSPPRRIGNAQKNAGHPALLSIGDKVWLAWLEHDA
                     GGYEAWAMTSLDGGRTWGPATVSLQSATKLDHPQWLNLQGKAMLAVNTVDKGLQLIPF
                     AQ"
     gene            16713..19295
                     /gene="clpB"
                     /locus_tag="VNZ84_08955"
     CDS             16713..19295
                     /gene="clpB"
                     /locus_tag="VNZ84_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HWU67127.1"
                     /translation="MRFDKLTTKFQQALADAQSMAVGADHPTIEAPHLLLAMLNQDDS
                     GVAALLSRAGAQVSALKTSLQQAVDDLPKVSENSGDVSISRDLNNLLNVTDKLAQKSG
                     DSYIASEMFLLALADDKGHIGKLLKQHGVNKSALEQAIKAVRGNDSVNSQEAEGQREA
                     LKKYTLDLTERARAGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPDSLKNKKVLSLDMAALLAGAKYRGEFEERLKAVLKELAQDEGQTIVF
                     IDEIHTMVGAGKSEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVLVDEPSVEATIAILRGLQEKYELHHGVEITDPAIVAAAELSNRYITDRFLPDKAID
                     LIDEAASRIKMEIDSKPEVMDKLDRRLIQLKIEREAVRREKDEASQKRFGLIEGEIEK
                     LEKEYADLEEVWKAEKAAVQGSAGVKEAIEKIKLDMEAAKRKGDWQKVSELQYGKLPQ
                     LEAQLKHASTAEASGVVKHKMLRTEVGADEIAEVVSRATGIPVSKMMTGEREKLLNME
                     SRLHERVVGQDEAVRLVSDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCKALANF
                     LFDSEEHLVRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGTLTEAVRRKPYSVVLLDEV
                     EKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIIMTSNLGSQMIQSMADQDYQVVK
                     LAVMGEVKTHFRPEFINRIDEVVVFHSLGEAHVKSIANIQLQLLAKRLAAMDMQLDIT
                     DAALAEIANAGFDPVYGARPLKRAIQSEIENPLAREILAGQFAAKDTIRVDFQAGKMQ
                     FRKV"
     gene            19384..19902
                     /locus_tag="VNZ84_08960"
     CDS             19384..19902
                     /locus_tag="VNZ84_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67128.1"
                     /translation="MHDLSYFRCMIAMTLLAGLAGCATQAPQPLQIDRISPEQLAALV
                     PPAQSTLSLDEVVAMGKQGKTDAEIIEAIKQSQSRYSLTPSEVLSWHQKGISKGVLDY
                     MQQANALAEQNAIADEINKREKARAESEAKLKRERDFARLRSMDPWFYGPGFYGGPWG
                     YRPYWGGGFRYW"
     gene            19960..20379
                     /locus_tag="VNZ84_08965"
     CDS             19960..20379
                     /locus_tag="VNZ84_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWU67129.1"
                     /translation="MRQQHETLSINAQGRRLYDITPQVLQWVQQSGIQSGLFTLYIQH
                     TSASLLINENYDRDVLVDMEAFFNRLVPDGDDLFIHTVEGPDDMPAHVRTALTQTSLS
                     IPVLQGRVALGQWQGIFLFEHRHLAARRRVLMHVMGE"
     gene            20427..20714
                     /locus_tag="VNZ84_08970"
     CDS             20427..20714
                     /locus_tag="VNZ84_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67130.1"
                     /translation="MKWITSLVAIFLALGLISCSQKEDVPVPETSPAAEPAAQQSPEP
                     VKSSGGGGYEPTADERVPGITMSQEEIDKQTAEALANTPMPDVPGESSSEK"
     gene            complement(20800..22182)
                     /locus_tag="VNZ84_08975"
     CDS             complement(20800..22182)
                     /locus_tag="VNZ84_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWU67131.1"
                     /translation="MSEFSIFALSKPSSRWAYCFTVCLCVWLGTLPLPAPAAANDKAE
                     TTKGDLEQVKEKIQQLANALKASRVAKQDANEALKASETAISASRKKLREIQDAQQEN
                     RGKLQDLQKQLSLLQRQVNQQRNTLSEQLNQQYRHGNNSPLQMLLQQQDPANTTRNLK
                     YLGYLTAAHKQQIQSLQDNQEAIDRVRAATTEQLHETERLANQYSETTQKLEGEKSRR
                     TEALNELSKQIETQEQQMVRLKKDEEALSQLFQRLLAEAKKREQEALAKAKREREAAA
                     KRAAELARKKKSAGRTFERSPQTNIEDSESNSSSSTVIAKNETLPEYSASKENFAQLR
                     GRLRLPVRGDVINRFGTARADTGVSWKGIFIRAKEGSEVKSIAGGQVVFADWMRGFGN
                     LIVIDHGNGYMSLYGNNEALYKSSGQAVKAGDTIAAVGNSGGNAENGVYYELRRNSIP
                     FDPLQWSTVK"
     gene            complement(22200..23750)
                     /gene="gpmI"
                     /locus_tag="VNZ84_08980"
     CDS             complement(22200..23750)
                     /gene="gpmI"
                     /locus_tag="VNZ84_08980"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HWU67132.1"
                     /translation="MSIKPVILLILDGFGHSETTEFNAVLQANTPNLDRLKATYPHSL
                     INASEHYVGLPDGQMGNSEVGHLNIGAGRIIFQDFERINNSISSGEFFQLPALVNAMQ
                     ALKANDKALHILGLLSDGGVHSYQPHIHAILDMAKQQGLSKVYVHAFLDGRDTPPKSA
                     QPYLQTLEDHLNSLGVGKVASVGGRFFGMDRDKRWERVSVAYELLVNGTSDYVAPDSL
                     TALQQAYARDESDEFVKCTAIRAAEEAPVRMEDGDCLVFMNFRSDRARQLTDALLNQQ
                     FTGFARSRIPHFSHYFTMTQYDKNQMLAEPIFAPYTVPNTFGEYVAKLGLKQLRIAET
                     EKYPHVTFFFNGGEETVFDGEDRILVPSPKVATYDLQPEMSAPEVTDKLVAAIESGQY
                     QAVICNYANGDMVGHTGNLQAAIKAVETLDTCIGRVVAAAQKMGAEVIITADHGNAES
                     MFDHASDQAHTQHTTNLVPFIYIGRPASITAGGALSDIAPTLLSLMGVPQPSEMTGKN
                     LITLDAMA"
     gene            23882..24292
                     /locus_tag="VNZ84_08985"
     CDS             23882..24292
                     /locus_tag="VNZ84_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWU67133.1"
                     /translation="MEFFKQNVLLIGLAIGSGVALLLPMLGRSAAGLTVLSVTEAVML
                     MSRKSALVLDVREADEFAQGHLQGARNIPLSQLESRSKELEKFRDKPVLLVCQRGSRA
                     NKAAKLLKSQQFSALNVLKGGMQAWLEAKMPTGK"
     gene            24315..24572
                     /gene="grxC"
                     /locus_tag="VNZ84_08990"
     CDS             24315..24572
                     /gene="grxC"
                     /locus_tag="VNZ84_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HWU67134.1"
                     /translation="MAKVVMYTSAVCPYCMNAERLLKNKGVTEIEKIRVDLQPELRAE
                     MMEKTGRRTVPQIFIGDQHVGGFDDLHALDVQGGLDPLLAA"
     gene            24666..25142
                     /gene="secB"
                     /locus_tag="VNZ84_08995"
     CDS             24666..25142
                     /gene="secB"
                     /locus_tag="VNZ84_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HWU67135.1"
                     /translation="MAQDSQNQTEQNAAPVFSIEKLYVHDASIEVPNAPAIFTERTTP
                     QINVELGNSAQQIEDGIFNVSIKVTVTAKIEDKTAFLVEVNQSGIFAIRNVPQENMEP
                     ILAVACPNILFPYAREAISDMVTRAGFMPVLLNPINFEALYLQQQQQAAQAAGAPN"
     gene            25201..>26184
                     /locus_tag="VNZ84_09000"
     CDS             25201..>26184
                     /locus_tag="VNZ84_09000"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWU67136.1"
                     /translation="MSKVAVLGAGAWGTALAMHIALQHDVVLWARNGGHVSGMRKARA
                     NPLYLGDFPFPEKLTVEDDLGAAIAGADLILSVVPTAGFRPILQQLKALGIDQPLIWA
                     NKGLEPQTAKLPFEVAQDELGSEYPWGVLSGPSFAAELVRGLPTAITLAANHRELAQQ
                     AAQLIHGGCLRVYDSVDVVGASVGGAVKNVMAIAAGISDGMGFGNNARAAMITRGLAE
                     ITRFGMALGAKAETFMGLAGAGDLILTCTGQYSRNREVGLQLASGKSLESILAGLGHV
                     AEGVNTAREVMRRAETIGVEMPITFEVNQLLTNQKTAQEAVTALLGRGQRQE"
BASE COUNT         6169 a         6264 c         7221 g         6530 t
ORIGIN      
        1 ggcagagttt tgtgtggcct tcctcctgac ccggagtaca aaggggttca tggatataat
       61 caattaaaat taaaacatcc ttttgatagt cgcgaagtgc cagttattta tggcgaccac
      121 gtgacgaccg atgccggtac cggcttggtc cacacggcag ccgcgcacgg taacgatgac
      181 tggctggtca tgcgtgctaa cttcccgaat gaaaaaccgc gcgtattgat tggtggcgat
      241 ggtaagttct tcactagtga gctggttgag ttggcagata tccgcggcct gagccgtcag
      301 gatgccaata aaattatcct ggctaagctg caggaaaatg gcgtgctgtt tgcctctgcg
      361 cgtttaaacc acagcttccc gcattgctgg cgccataaaa cgccactcat gcaactggcg
      421 acgcaccagt ggtttatcgg catgaactcg gtcggtaaag atggtaaagc tttgcgcgat
      481 cttgcaaata tgtctgttga tgcgacgcaa tttttcccaa gttggggccg tgcacgtctt
      541 gaagcgatga tcaagaaccg cccggactgg tgtgtgtcac gtcaacgtaa ctggggcgtg
      601 ccgatgccgt tttttgtgca taaagaaacc ggtgagccgc accctcagac catgcagttg
      661 ctggaaaccg tgcgcaaaca ggtggaacaa cagggcattg aagcttggtt cagcttagat
      721 ggtgctgctt tcctggcgca gcatgcgcca gcgaatgccg agcagtataa aaaagtcacc
      781 gatacattag atgtctggtt cgattctggc gccacgcatt acgccgtggt gcgttcagca
      841 cggcacccaa gcttgtccgc tgccgcgaat gtgcgcgaag ctgaacacca gcctgtggca
      901 gacttgtatc tggaaggttc agaccagcac cgtggctggt tccagtccag cttgctgacg
      961 ggttgcgcca ttgacggcaa cgcaccttac aaagccttgc ttacacatgg ttttgtcgtg
     1021 gatggcgccg gccataaaat gagtaaatcc aaaggcaatg tggttgcgcc acaaaaggtc
     1081 atggatactt atggtgcgga tattctgcgt ttgtgggtgg cttcgacgga ttactcaggc
     1141 gagttgacca tcagtgatga aatcctcaag cgagtggccg atggctaccg ccgtatccgc
     1201 aatacactac gtttcctgct atctaatctg gctgattttg atgccagcaa agacctgctg
     1261 cccgtcgatc agtggctgga aattgaccgt tatgcgctac atctgacaca gcaattgcag
     1321 acaggcatcc tggcggacta caataaatac gaattccact tggccgtgca gaagtttgtg
     1381 agtttctgtt cggaagacct gggtggtttc tacctcgata ttctcaaaga ccggttgtat
     1441 accagtggtg aaacttcaca tgcacgccgt gcagcacaaa gtgccttgca tcacatcacg
     1501 catgccatga tgcgcttgat ggcgcctatc ctgagcttta ctgccgatga aatctggcag
     1561 actttgggtc tggataacgt cgcgacggtg tttgaagaag attggtatgg tttgccagct
     1621 tgtggcttga gcgaatccgt gctcgctgat tgggaaaacc tgcgccagtt ccgcgatatt
     1681 gctaacaaga aaatagaaga aaagcgtggt gaaggcgtgg ttggttcttc gttgcaggct
     1741 gagctggata ttcatgtgga tggcaacctg tatgattcac tcagtcgctt tggcgaggat
     1801 ttgaaattcg cattgattac ctcacgtgcg aacgtttata agcaggtcgg tgccggtgtg
     1861 agtgtagacg tcaaatcaag cgcaaatgca aaatgcgacc gttgctggca ttaccgcgct
     1921 gatgtgggga gagatgctgc gcatccgacc atttgcggcc gttgcgtaag caacctgttt
     1981 ggcaaaggtg aagtgcgcac ctatgcctaa attgcgctca gccttaccgt ggttactatt
     2041 gagcgcgatt gtgatcgcgc tcgatctcta taccaagcac ttggtccaac aagcgtttgt
     2101 ttatggagag catgtgccag tgaccgggtt ctttgacctg gtccgctatc acaataccgg
     2161 cgcggctttc agctttttag cgaatgcggg tggctggcag catggcttct ttacggcggt
     2221 gtctaccctg gcctctgcag tgatggtgta tctgctggtt aagcatccag agaaaagatt
     2281 gttttgcttt ggcttggcgt tggtattggg tggtgctttg ggcaatcttt atgaccggtt
     2341 gactttgggt tacgtggtgg actttttgct gttttattat cagcaatggg catggccagc
     2401 ctttaatgtc gcagatagtg ccatttgtgt cggggtcgct ttgctgatgc tggatagttt
     2461 taagcaaccc aatacgtaat tgctctaaga tactccaaac ctcaggaaag ggcgctgtga
     2521 tggatgggtt gagctatcgc ttcaatgatg tcatcaagct ttgggttggc agtaaaagta
     2581 cctggcatta cgtcagtttg ccacagggga tgtccgacga aattcatacc ctgagccaat
     2641 atcatcaaac caagcgtcgt ggctggggcg cggttaaagt ggacgtcagc gtgaaggatc
     2701 agcaatggca aacctccatc tttcctgcgt ttgacaatca acgctatgcc ctgtttttaa
     2761 aggcggatgt acgcaagcag ctgggtgtgg ctgttggcag cgaagtcacc gttacattga
     2821 aaattctttt ttaggcgtca tgggctcggc ttgtgctgtt gccaacaact cttgaatatc
     2881 ttctattggc attggtctgc caagcaaaaa gccctgaatg caatcacagc cgacctggcg
     2941 taagaagtcg agctgggcct gagtttcaac gccctcaacc acaaccgaaa tgccgaggat
     3001 gccagctgtt ttgatgatgg tgctcacaat ttcactgtct gcactgcttt ctgggacatc
     3061 caccataaat gcacggtcta tcttgagttc atcaatcggt aactgcttga gtttaaatag
     3121 tgacgaattg cctacgccga aatcatccag tgaaatgcgt acgccaagtg ctttgagctg
     3181 tttaagaatt tccaaggcct gttcttcatg ctcaaacacg gcactttcag tgatttcgag
     3241 ggtgatgcat gcgggcggga tggcatattg cttcaagtgt tgagcgatcc ttagtggcaa
     3301 gctatggtca cggaattgtc ttggcgagat attaatcgac agtccaaccg gttggctgct
     3361 tttgctggag agtgccgcca attggcggaa ggcttcggca actacccact cccccaatgg
     3421 caggatcagg cctgactgct cggcgtaagg gataaattcg cctggggaaa tgatatcgcc
     3481 tttatgcgtc ttccagcgga tgagtgcctc aattttggcg atgcgcggtt gggtattttt
     3541 atcaaggcaa aaaataggtt ggaattgcag aaagaattcc tgtctgcgaa tggccttgcg
     3601 caattgcaca tccaacttaa accggttctg ggccagtttg gtttgttctt tactatagaa
     3661 catgaataaa tgttggctgg aacgcttggc atgagccagg gcggtattgg cattccgtgc
     3721 aagctgttcg ctattgttag cgtcccttgg gtaaaggctg atcccgatgc aggcatccaa
     3781 tatcagctcg tggttttcaa tggtaatagg tttatgcacg attttgcaga ggttgtgcgc
     3841 caggtgcacc agctccgcat cggttttacg ccgtgtgata atcagtgcaa acttgtcacc
     3901 gtgcacgcgc cccagttcac cacagtcatg ggcaatgtat tgcaggcgtt gcgcggtttc
     3961 aatcaaaaag aggtcaccgg ttttgtggcc aaaactatcg ttaatgcttt gaaaatgctt
     4021 caggtctatc attagcaagg ccaccatgcc actctcgctg gcagcgccaa taatggcatg
     4081 ctccagtaaa ttcagcatgg caagccggtt aggcaggtgg gtaagaggat catgccgcga
     4141 caactgctgg tactccagct ctttttcagc aagtcgtttc tcggctcgca ataaccgctc
     4201 actctcttgc agcaaaatgc tctgccggat agttgcaaaa ataaggatga cggttaccga
     4261 accgatggca atatgatacg cataagggtg tgggccgctt tgccaccaga tgagcattaa
     4321 tgtgctgatg ctgaatgtaa aagcgatcac cggaatccag ctacggagcc gtcggcactg
     4381 tcggtgataa accgcacttt tggtgatacg cacttgccag ccatagaggc cggtcgcgat
     4441 aagcaggtca gcagcagaga atccatagtt gatccacaaa ctggtttgcg gcgtgcggtt
     4501 gagtgcgttc aggttccatt gcatccagca tatgccgagc agtgagagtc ccgcactcag
     4561 cattaaccag ggcttcatgc aggtgggcct taaatagggg atcaacaaca gtgaaaccag
     4621 cgccgcgctg agcatgctgg cgggatatgc ggtgagtacc gttaattcca gcgggctgac
     4681 tgctgtggcc tggggcaagt aagaaatcaa gatataccct atcacggcga cactaaagga
     4741 gagggcatct actgtaaagg ccagcgtttt agcgcgggac agctggctgc gaagggcaac
     4801 cacaaagcct atgatgaggc aggggcccat caacgggtaa aagagatcag attgtgccgg
     4861 aaaagtggtt tgatcggtca ggacttcaaa tatccataac aggccgccgg taaagtaaga
     4921 aagcgcacca atcacaaacc agcgtttgca ggggcgatgt gacggattgg cacgcaggta
     4981 gccgagtgtg gcaaggatca ggcaggcggt aatggtggaa aaccaatgaa tgttatccag
     5041 ccataacgcg gtttcgggcg ttgcgttaat tgttgccagc aggcaaacca atgaaacaga
     5101 taaagcaaca acaatgactt gacgagaaag tgtcatgaag cccccttgtt tttaccaatt
     5161 ctaacaaggg gttagaaaaa aactagtccc tcctgagaaa taacttcacg agttcaacca
     5221 cggtaaaaca gattgcaccc atcagcacgc ctatagccgc attgatcaat aaagaccagt
     5281 gcggtaattg ggcgaccagc gcgtgcagtg ggtgaatgcc atgtgcaaga atgccgcccc
     5341 cgaccagaaa catggccgcg gtgccgagta cactgagcaa gcgcatcaaa gaagggacaa
     5401 cttgcaccaa gccactgccg aattttcgtg tggattgtgg caatacccct tggccttgtc
     5461 gctgcataag ccacaggcca atatcatcca gtttgacaat cagggcgacg atgccgtaga
     5521 cgccaatggt catgagcaca gcaattaaag acagcacaac gatgcgttgt gtcagcgccg
     5581 cttctgcaac cacgcccaat gcaatgacaa tgatttcggc tgaaaggata aagtcagtgc
     5641 gcacggcacc tttgattttt tccttttcaa gcgcaactaa atcggttttt ggctgttgca
     5701 gggccgcgtg caacacctgt gtatgcacat cttccttttc gatatgacga agcttatgcc
     5761 aaattttttc aaagccttca aaacacaaaa aggctccgcc cagcatcagt agcggcgtga
     5821 ttagccaagg cagaaatgca ctgattaata aggccgcagg taccagtatc agtttgttaa
     5881 tcaatgaacc acgtgccacg gcccaaacca caggcaactc tctttgtgcc gcaatcccgg
     5941 tcacttgctg ggcattgagc gccaggtcat ctcccagtac gcccgccgtt ttcttggcag
     6001 ccaactggct catgaccgat acgtcatcga gcatggtggt gatgtcatca agtaatgcca
     6061 ataggctgga tgcggccatg gtttgtcctg tgtcaattga aatgcttgtc tattctaaag
     6121 gattgtcgtt aaaatgccgg gatgataacg gtcaaatttc aacgttggat ggcgcgcatg
     6181 gccttgatgg caatcatgct ggccacgctt atgccaacat tgaccgtggc tttccccatg
     6241 caaagtggta aaacgtttgt gcaggaagtg tgttcttcac agggcaacaa actcgtcatt
     6301 caggtgctga ccacacaggg caaactgcgc gccaccctga ttgattatca gccttcgcaa
     6361 aaaccaactt cattaagcca ccacctcaac cattgcccgt tctgccagat ggcgctggat
     6421 caggcgacca tcccatcgca taatccttct tatattctgt ttcagaaagc acaggcgcaa
     6481 atcgctttaa gcgagtatca ggcacctgtt gtttcaagtt tctatcctac tgcgcatctc
     6541 tcacgcgcgc ctccgctgac tccacactca gtctaattcc gtcagttggt ttgacggctt
     6601 atgagtctgc ctgcaagcca ggcatgaatg gagtctcaca tgattattat caatctacag
     6661 gcaatgattt ttgccaaaca ggtggagtgg aaacgacaca tcaccctcaa agaagtcgct
     6721 gacgccaccg gtatcagccg catgaccctg caccgcatgg tcaagaatcc tacctataac
     6781 gcttgcactg agcatcttgg caagttgtgc acctattttg attgtgacat cagcgctttg
     6841 atccgctggc agccaaacac ggctgtcagg caaatcattg ccgcctgatt cactattcac
     6901 ctgtttgcct gacggtgagc aggtgtgcaa gtaaatattg aggaataaca aaatgaataa
     6961 aacaaaaata ggcttggcca ttttatccgc tctgccatac ttggcgcacg ccgaacacga
     7021 gggcactgtg caccttgatg aggtgaaagt agtggctaag aaaattaact cttcaaccac
     7081 gcaaccggat atcaatacag caaaggaaaa tttgtcaaaa actgcaggcg gaaccactgt
     7141 ggtagatatg gcaactgtgc gtgagggccg taccagtaat atgcaagaca cgctgggtat
     7201 ggcaactggc gtactggccc agtcccgctt tggtgcagaa gaaacacgcc tatccattcg
     7261 tggatctggc ttgcagcgta cctttcatgg tcgtggcttg aaactcatgc aagatggtat
     7321 tccgaccaac ctggcggacg gcggctttga ttttccggcg atcgatccga tggcaaccga
     7381 ttatattgag gtgtatcgcg gtgcaaatgc cttgcaatat ggctcaagta atttgggcgg
     7441 cagtattaat tttatttccc gtactggtta tacagccccg gcgctggaag tgaaaactga
     7501 gggtggaagc tatggctatt accgtcttgg tgtgagtacg ggcggtgtga aagaaaatgt
     7561 ggattatttt gtgactgcca gcaaatacca cacggatagt tttagggaca atgcccagca
     7621 aggcgcagac cgcttgactg gcaatgttgg tgtgaaaatc aatgaccagg tcgaaacgcg
     7681 cttttacttt ggttatgtga ataatgattc acagctttca agcagtctga ccaaggccca
     7741 gttaaaagac aatcccaaac aggccactgt gattcctggc cagggcatta acgaacgtaa
     7801 tattaacgtg acccgactgg caaacaagac gacgatttta tttgatagta gcaagttgga
     7861 gttgggtgca ttttatgcac acaagagctt gttccaccca attggcaatt ttggggatac
     7921 cacgggtgtg attgatcagg tgtcaaatga tattggctta accgcgcgtt ggagccatca
     7981 gggtgagctg ttcggcatgt cgaatgagtt cgtggccggt ctttcgccaa ccagcggtcg
     8041 aatagacgct aaaaactacg taaacgtgaa cgctagacgg ggcgcattac tcaacgactt
     8101 tgaccagaag gcgaccaact ttgagagcta tctggaagac cgtttaagcc tggatgatca
     8161 atggacagtg atcgccggat tgcagtatgc gaaatccaag cgcgactcta aagaccaatt
     8221 aatcactgcc agtggtgatc agagcgtcaa taaaacttat agccagacga gccccaagtt
     8281 gggcgtgttg taccagttgc agccacaggt gcaagtgttt gcaaatgcct cgcgtagttt
     8341 tgagccaccc agttttggcg agttgaatag cctggtggtg agcagtttga aagcgcaaaa
     8401 agggacgacc tttgaaattg ggacacgcgg gaatagtgag tttgttgact gggatatcgc
     8461 actttatgat gcgcgtctgc aagacgaatt gcttgcagtg gctgcagcag gatcgccggt
     8521 tgctacgcaa actgtgaatg ccggaagaac catccaccgt ggcctcgagt tcggcctcac
     8581 tgcacgcctg ccttatagct tgcaatggcg ttccagtttg ttggttaatg actttaaact
     8641 ggacaatgac cccacttatg gcagtaatcg cctacccggt atccagcgtt cattgttgcg
     8701 tgccgagttg gtttaccgtg gcagtgatgt acttgaaaac ttctatatcg gcccgaccat
     8761 tgagtggtcg cctcagcgct acaacgttga ttttgccgag accctgtatg cggacagtta
     8821 ttttctatgg ggcatgaaag caggtcagaa agttaaccag cactggtcct ggtttgttga
     8881 ggggcggaat ctggctaatc agaagtatgc tgcgaccacc ggtgtggcaa gaaatcagaa
     8941 tggggcggat ggtgccctgt tcctgccagg tgatggtcgt accgcttacc tgggtgtgac
     9001 ctggacatat tagtgacgaa ccggggtgcc tggtaccccg ggtgatggag tgtttaaatg
     9061 aaattaatct tgaagttcag cctgttgttc attgcgctga gcatcgcccc tgcgtatggc
     9121 cactcaacag ctgccgcaga cgacgaacaa tcggttgcaa aagaaatgca tgaaacctgg
     9181 gacaagccta accagccttt agtgctgccg gtgattgtga ttcatcacgg catggctatt
     9241 gcggactgga tccagggcga taaaggcggg cgtgctttgc tgaagtatca tcctgagcat
     9301 cagcattggc ataccctgct ttgtagtggg gcggagttga cggcacctcg gcatttgcag
     9361 gaggccggca tgtcagcggc tgatgcgcag tcgctcgcac ttttactgca acagcaagag
     9421 caggcgttgc cggacgatca gcgccaacag atagacagct tcagtggtgt ggtgaagttc
     9481 agcaaaggca aaccagtatc gtcagtgggt catgaaatgc atggtgaggc acatggccat
     9541 taaaatatct acagttaaaa accagtggtt gttctggcac cagtggatcg ggattgttgc
     9601 ctgtattggc atggtgttgt ggggattgtc gggcgcatct cacccgctca tgagccgctt
     9661 gcaaccggtt cctaaacagt tcatgccgcc cttggtgccg gtcgacatga gccaggcagt
     9721 tgcacttccg ggcttgttaa aacaacaggg gatcacccag tttcagcata ttagcctggt
     9781 gcagttagaa ggtcagggct attaccggat tgcgcaagct ggggcagttc gttatttcgc
     9841 catggctgat ggcgcagagc gcgccgatgc ggagcaagcg ctggcggtca acttggccaa
     9901 ttactatacc ggacgtaatg atccttccat atgggctgta aagcccgtca cccagtttga
     9961 gccggattac catccggtga atcgtttatt accggtttgg cgtgttgctt atgatggtga
    10021 tgagggcttg cgtgcctatg tggatactga gcaatccagg ctggcgacgc tggtggatga
    10081 ccgacgcgct ttcttgacca aggtattcca gtttggccac aactggtcat ttcttcaggc
    10141 gtgttcaacc ttgcaattag ccgtggcaac cttggtgttg gtgttgattt tggcttctgc
    10201 cgtgaccggc gtggtgctct atatcaagca acgcaaaact gcacctttcc ggttaaagcg
    10261 ctggagcttg cagtggtggc accgggaatt tggcgtatgg gtgtcgctgg tcatcttatt
    10321 attagccagc acgggtttgt tccatttgtg gatgtctgat atgcagcagc gcaggcccgg
    10381 catgcctacc gtatacgcca gtgaaagcgt tgcactcagt gaggccgcct gggcgaaagt
    10441 cgcgactggc caagtgttaa agctggatat ttatgggcgt ggcgcaacgt tattctggca
    10501 agtgatcccg gtggtcgccg gggagcaggc tttgccatca gcacaggtgg cggccatgca
    10561 tcatcatgac catgcagggc atgcacagcg tccggcggag ccagtgttat ggttgacgcg
    10621 cagtcaggaa acaacggtac tgactccaca ggcgtatgct tcggcctggc tggcagtcat
    10681 ggcaggcaac ccttcgcctg ctattcaatc ggtcgcttgg gtcacgtcgt ttgccaatga
    10741 gtatggtttt gtctttaagc ggttgcccgt gttaaaagta caaacggcgc agactgagca
    10801 cacacgttat tacctcgagc ctgcgacagg tatgctggca gcgacgatca atgacaccga
    10861 tggtttggaa ggttttgtgt ttgcttatct gcacaagtgg agcttccagt ctgtcagcaa
    10921 ggatgtgcgt gatttgctgg ctgtcatcgc agcgctcgcg gtcagcctga ccgggctgct
    10981 cggcgcttat ttatttgcga aaaagaggat gtgacgcgta gtggctggcc catcgggcca
    11041 gcgtagaagc ggactatgag gcagcgtaaa ttttgtccag aaagtagctg gtacgctgtt
    11101 tcaaccgcaa catgctctgc agggcatttg gtaactgctc tggtttcagg cattgaatga
    11161 tgttgatatc caaggcttcc ctgaggtcga tgatttcacc ttcttcctcg cggtcatcgc
    11221 tgagcatttg aatgaatggc cgcaaaggtt gctgtggatt ggttgaaagt actgtcgcca
    11281 gcttctggtc ggaaagtcgc acgacgctac cgggcgggta aatgcccagg catttgataa
    11341 accggcgcaa gattgactgg tcgaactggt gtcgttgttg cgcaaacatg acgctcaggg
    11401 cttcataagg tgttttggtc agggccgggt tggatggatt gcacaggtta tcgtacatat
    11461 tggcaataat caggatgtgc gataacgggt caatctggtc gcaatgcaac cctttgggat
    11521 agccgctacc atcagcaaac tcatggtgtt gcgaaatcag ggtgagtatc cttctgggga
    11581 ccgccacgtc gcgtaacatg ttcaggccaa gcaggacatg ggtctggtaa atggaagttt
    11641 cgcgcacact gagcggatct ttcttgagca ggatcttgtc gctgatctgc cgcttgccaa
    11701 tgtcatgcaa gatggctgcc atgcctaaaa cgacagcttc atcgtcagtg atgtccaggt
    11761 ttttcgccag caacatggcc accaccaggg tgtttaattc atgctggaag tgctggtcgc
    11821 cgatacggtg gccattcatg gcgtgaatcg ccacgtcacc ctctgtgagc gtactgctaa
    11881 ccaaatcttc taccagtctt gatgccagcc cataagcttg ttccggacgc tctgttgaca
    11941 gtttctggat gtttttaact tcggaactgg tcttcatgaa cttctgttca ctttcagcaa
    12001 tcgcttgcct gagtgccagc tgtttttttt gtgcagggct taattccggg gcatcatcaa
    12061 tgatttccag tctaggggag gtggccttcc gcagcggaaa ttccaccaca ttctggtaag
    12121 cagcggccct gagcggtgtg ctggaactgc gtcttgggtc ataccgcagt tgcttcaatc
    12181 ctaacgatct gatggtttca atttgcgact catcggtaat tttgaagttg ctgaccgaaa
    12241 acggatgatc catccatccc aaatcaagat ggatgtatag acccacctgc agctgatcta
    12301 tgtctatgta gtaagtgtcg gaggcgtgat taggcatggc gttttatttt tctttgattg
    12361 tttaagacta ataactgcag aaaatcctct gcattgactg gataagcata caagtaacct
    12421 tgtgcagtct ggcagcccag gcgctgtaaa aacttttcct gttcttcggt ttctacaccc
    12481 tcggccacaa cgctgagtcc cagctgggtc gccatcgcgt agatagcact ggtgatggcg
    12541 gcatcgtctt tgtcatctgg caagtctttg acaaaggagc ggtcaatctt tagctggtcc
    12601 aggggtaagc gtttcaggtg tgacagggat gagtagccgg tcccgaagtc atcaatagca
    12661 atgctgacgc ccagcagttt caactggtta aatgtatcca ctacatgctg ggtatgctgc
    12721 atcagggtac tttcggtcag ttccacctct atgatggaag ggtcacactc actgtccatc
    12781 aatatgccat agacggtatc ggcaaacttg ctcttcatga tttgcacgcc tgagacgttg
    12841 atggcgatct ttttgaactc agggtgtttt ttgtgccatg tatgtgcctg acgtaccgct
    12901 tgggtcaatg cccattcgcc tatgctcaaa atcagaccgg tttcttcagc cagtgggatg
    12961 aattttacgg gggagatcat gccctgggtt gggtgttgcc agcggatcag cgcctctgcg
    13021 ccaataatca tgccggtttt caagtcaatc tgtgcctggt agaacatttt gagctggttt
    13081 ttagtgattg cctgacgcaa ctcggtttcc agctgaaagt gttcaacggc atcccggctc
    13141 agtgccgcat cgtaaaaagc aacatggttc ttcccgttgt ttttcacctg gtacatggcc
    13201 aggtctgcag ccttgatcag gccttccacg tcttggccat catgcggata catggagata
    13261 ccgatactgg cgccaataaa cagctcatgg tggtcaatca tgaacgcgcg tctaagcgag
    13321 tccaggatgt catgggcttt ttgcgttgca tccgctttac tgtgcaggtt ttccagcaag
    13381 acaataaatt cgtccccacc caggcggccg acaatgtcag actcacgcag ggaggcgcgt
    13441 aatcgctcgg tgacgcccag caagagctgg tcaccagagt aatgacccag actatcatta
    13501 atgtttttaa aacggtccag gtcaataaac agtacggctg cctgatgccc attgcggtgt
    13561 gcacgctgga ttgcatgggt aaacaggtga ttgatcagcc ggcggtttgg taagccggtc
    13621 agcggatcat ggttaaccag cgaattcagt tgcgtttgtg aacgcttcat ttcgctgatg
    13681 tctgcaaaca ctccaacata attggtaatc acattggcat ggttgcgtac tgcagtaatg
    13741 gtcatccact cggtatagct gtcgccattt ttgcgcatgt tctcaatttc gcccgaccat
    13801 ttgccatgac gtttgagcgc gccaaacagg ccgcgcgggc gctgtctgaa tatttgtggt
    13861 ttggtcccca gcacttcaga ttcttcaaaa ccggtgatca gggtatatcc ctggttaatc
    13921 cccaccagtt cgcccgccgg gttcacaatc ataatgcctt ctgtggtgct gtcgaaggcg
    13981 attttagcca gtttgaggtg tttgccagtg cgttcgcggt caatcgccac gctgagtgtc
    14041 cggccaatcg ccatcaatga gcccagttcc tgtgagctgg tgttctccgc atcaaagcac
    14101 cgctcaatga ccatggatcc ccaccactcg tcatcgatca tcaatggcac aatgaccagg
    14161 ttaacggtat cgtgcgattt aagcagggcg cgctctgttt ccgggagtgt gagtgcatca
    14221 cacatcacgg gctgcccttt ggatagcagg gtgtaccagc gctgtaattc cggtgacagg
    14281 aaagaaattt tttcccagtg caggtaggtg ttatggaaac cattaattgc ccaatgaaac
    14341 attgcttttg agcggatctc ggtttttttg tgcgtagaaa tggtttcgtg ctggagcaac
    14401 accgcacggc taaaacggaa agacaggcag cattgttgta atacgttttc tgccacgctc
    14461 tgccagcgtt cagcggaatg tagcatccag tcacatgaaa tcagggtatc caggactgcg
    14521 ctgcggttct gtaactgtgt gtcaatgaat ttctgttcgc tcacatcccg cgccacggta
    14581 atcaggtggc tgggttggtc tttttggtcg tggtaggcaa ttttgttgac cgccagagtt
    14641 ttatggccac cttgcggtaa atgtaccagc tcttcattgt ggtaagcctg ccgcgtttgc
    14701 cacaccagct gatcggactt ttcatggtgt tcgaagctgt tacggaacac gggatgcaag
    14761 gtatctgcaa tctgcaaatt attcaggaat tcatagtcaa aaccatccat ctggaaagta
    14821 ttgagcatgt gctggttggc catcatccag cggttttcat gatccttgat gcaaataaga
    14881 tccggactca tttcaatcag ggaatgcacg atggactgca tatgccgtaa ctggtcggta
    14941 agcgtgcgtt gcgtttcctc taacatcagg cccactgcac ggcgatcaga tggcggataa
    15001 aacaggcggc atttgaattc acgttttaag ccatgtaccg agagatggat gttcagttct
    15061 atctgtcctt cacgcatggc ctgtaccagt gcctggcgga cttccttggg caacgattcg
    15121 gcatggtctc ccagcaacgc ttgagcacgg gcgctggccg ctacacactg cgcgcggcgg
    15181 ttgagtgcca gcatcgtcgt atcaggcatg gtggcgacca gaggctgcaa ccatgctgtg
    15241 ctatgatgat caaaaacggt tggtaatgac ataattaaaa tctgcttcgg tgcgaggtct
    15301 atgtgttggc ccggataagt gggttaacgg cgcatacgat accaaacttt agccagatta
    15361 atgcgtgata acaaaagtta atttggccat gcttttgata aggagtcctg agtgaaacag
    15421 ccgatatccc aatggttatt ggccagtgtt ttgtgcatgc tgcccctggc gcaagcacat
    15481 gaaaaccatg ctgaacatac tggccatccg ctggccgtta gtgtggttgc agatgaggct
    15541 gggcacgttt tggtcgcgca ggtgaaacag ggccaggtga tggtcgctgt cagtgaagat
    15601 gcaggccagc atttcaatca gggagtcgct gtcaatgcta cgcccatgca ggtggcggcc
    15661 gatgccgaag cacggcctca tatcgccgtg ggtcctgatg gcaacgtcta tgtcacctgg
    15721 accgagtccc tcagaaaatc ctatgccggc aatatctggt ttgcgcgttc tgtggatggt
    15781 ggaaaaagct ttgaaccacc ctatatcgtc catcagcacc gtgaagaaat cacccaccgt
    15841 tttgatgtgt tgcaagtgac tgcaaatggc agcattacgg tcgcctggat cgataaacgg
    15901 gacgcgctgg ctgcaaaagc ggctggcaag ttttatgagg gagcagccat ttattatgcg
    15961 gtttctaatg atcacggcaa aagcttccat cccgagcaaa aactggctga cagcagttgt
    16021 gaatgttgcc gtattgccat gactggcaag gcagatggta ccgtggccgt gttatggcgc
    16081 catgtgtttg atgatggtga acgtgatcat gccatggctg aaattagtcc cggtgccagg
    16141 ccagtactgg tgcgtgccag ttatggccac tggaagctgg acggctgccc gcatcacggc
    16201 gcagccctgg ctgtgggtga aggttttggc tatcacctgg cttatttcga tggagcaggc
    16261 aacaggccag gcttgagagt tgcccgcatg gatgatcatg cctgggtgac ttcaccgcca
    16321 aggcgtattg gtaacgctca gaaaaatgcc ggtcatccgg ccttgttgag tatcggtgat
    16381 aaagtctggc tggcatggct ggaacatgac gctggcggct acgaagcctg ggccatgacc
    16441 tcactggatg gcggccggac ctggggcccc gcaacggtct cgttgcaaag tgctaccaag
    16501 ctggaccatc cgcaatggtt aaatctgcaa ggcaaagcga tgctggcagt gaacactgtt
    16561 gataaagggt tacaactcat tccctttgct cagtagcaat acaaaatctt acaaatttga
    16621 tctatatcta ttgaaaacgc taaaacccgc cgcattatag ggttatccag ccgcaagcgt
    16681 gcggcatgac ttaaataatg aaagggtttg taatgcgttt tgataaactc acaaccaaat
    16741 tccagcaggc actagccgat gcgcagagca tggccgtggg tgccgatcat cccaccatag
    16801 aggcaccaca tttgttgctg gcgatgttga accaggatga cagtggtgtg gctgccttgt
    16861 tatcgcgggc cggtgcgcaa gtcagtgcac tgaaaaccag cttgcagcag gcggtagatg
    16921 acttgccaaa agtcagcgaa aacagcggcg atgtcagcat ttcacgcgac ctcaataacc
    16981 tgttaaatgt cactgacaaa ctcgcgcaaa agagtggcga tagttatatt gccagtgaaa
    17041 tgtttttgct ggcattggcc gatgataaag gccacatagg gaaattgctc aaacaacacg
    17101 gcgtgaacaa gtctgcacta gagcaggcaa taaaagccgt gcgcggtaat gattccgtca
    17161 atagccagga agccgaaggg cagcgcgaag cattgaaaaa atacacgctg gacctgaccg
    17221 aacgtgcacg tgctggcaag ctggacccgg tgattggccg cgatgatgaa atccgccgcg
    17281 cgatccaggt cttgcaacgc cgtaccaaga ataatccagt cttgatcggt gagcctggtg
    17341 tcggtaaaac ggcgattgtt gaaggtttgg cgcagcgcat cgttaatggt gaagtccccg
    17401 actcactcaa gaacaaaaag gtattgagcc tggacatggc cgctttgctg gccggggcca
    17461 aatatcgtgg tgaatttgaa gaacgcttga aagccgtgct caaggaactg gcacaggatg
    17521 aagggcagac catcgttttt attgacgaaa tccataccat ggtaggtgcc ggtaagtctg
    17581 aaggtgccat ggacgcgggc aatatgctga aaccggccct ggcacgcggt gagttgcact
    17641 gtgtaggcgc aaccacattg gatgagtacc gcaaatacat cgaaaaagat gctgcgctgg
    17701 aacgccgctt ccagaaagta ttggtcgatg aacccagtgt agaggcgacc attgccatct
    17761 tgcgtggcct gcaggaaaag tatgagttac accacggcgt ggagattaca gacccagcga
    17821 ttgtggccgc tgccgagctt tccaaccgct acatcacaga ccggttttta cccgataagg
    17881 cgatcgacct gatcgacgaa gcggcttcgc gtatcaagat ggagattgat tccaaaccag
    17941 aggtgatgga caagttagac cgccgcctga ttcaactcaa aattgagcgc gaggcggtgc
    18001 gccgtgagaa ggatgaagcc agccagaaac gatttggcct gattgaaggt gagatcgaga
    18061 agctggaaaa ggagtatgca gacctggagg aagtgtggaa ggctgagaaa gccgccgtac
    18121 aaggctctgc cggcgtcaaa gaggccattg agaaaatcaa gctggatatg gaggccgcta
    18181 aacgcaaagg tgattggcaa aaggtttctg aattgcagta cggcaaactg ccccaactgg
    18241 aggcccagtt gaagcacgct tctacggcgg aagccagcgg tgtggtcaaa cataaaatgt
    18301 tgcgtacaga ggtgggtgca gacgaaattg cagaagtggt gagccgggcc acagggattc
    18361 ctgtgagcaa aatgatgacg ggtgagcgcg aaaaactgct caatatggaa agccgtttgc
    18421 atgagcgcgt ggtcggccag gatgaagctg tgcgtttggt ctcagatgct atccgccgtt
    18481 cgcgtagtgg cttgagcgat cctaaccgtc catatggtag ctttttattc ctgggcccaa
    18541 ccggtgtcgg taaaaccgag ttgtgcaagg cactggccaa tttcttgttt gatagcgagg
    18601 agcacctggt gcgcatcgac atgagtgagt ttatggagaa gcactctgtc tctcgcctga
    18661 ttggtgcacc gccgggatat gtgggctatg aagaaggcgg caccttgact gaagccgtgc
    18721 gtcgcaaacc ttactcagtg gtgctgctgg acgaggtaga aaaggcgcat ccggatgtgt
    18781 ttaatgtgtt gctgcaggtg ctagatgatg gtcgcctgac cgatgggcaa gggcggacgg
    18841 tagactttaa aaataccgtg attatcatga cctctaacct gggtagccag atgatacaaa
    18901 gtatggccga ccaggattac caggtggtga aattggccgt gatgggtgag gtgaaaacgc
    18961 atttcaggcc ggagttcatt aaccggattg atgaagtggt ggtgttccat tcattgggtg
    19021 aagcgcatgt gaaatccatt gccaatatcc agttacaatt gctggctaaa cgcctggccg
    19081 cgatggacat gcaacttgat attacggacg cagcactggc tgagattgcc aatgccggtt
    19141 tcgatcctgt ttatggggca agaccactca agcgtgcgat tcagagtgaa attgaaaacc
    19201 cgttggcaag ggaaatcctg gcggggcagt ttgcggccaa ggataccatc cgtgtcgatt
    19261 tccaggcagg caagatgcaa ttccgcaaag tgtgaacgcg tttctcgatt aactgaacct
    19321 tgagccgcaa caaagggtcg tactcgtatg gccctttttt tgccgggtat aaatggaagc
    19381 cttatgcatg atttaagcta tttccgttgc atgattgcaa tgacattgct cgctggtttg
    19441 gcgggctgtg ccacgcaggc accgcaaccc ttgcaaatcg accgcatctc acccgagcag
    19501 ttggctgcgc tggtgcctcc ggcgcaatcc accttaagtc tggatgaagt ggtggctatg
    19561 ggtaaacaag gcaaaacgga tgctgaaatc atagaggcca tcaagcaatc acaatcacgt
    19621 tattcgctga cgccatcaga agtgttgagc tggcatcaga aagggatttc taaaggcgtg
    19681 cttgattata tgcagcaagc caatgcactg gcagagcaaa atgccattgc cgatgaaatt
    19741 aacaaacgtg agaaagccag ggcagagtcc gaagcaaaac tcaagcgtga gcgtgatttt
    19801 gccaggttac gtagtatgga tccgtggttt tatggccccg gtttctatgg tggtccctgg
    19861 ggctaccggc cttattgggg tggaggcttc cgctactggt aatgtgggtc atgggtgccc
    19921 ggtataatag aggtttggtt tttggttccg ggagtcatga tgcggcaaca gcacgaaaca
    19981 ctttctatca atgcacaagg gcgtcgttta tatgacatca cgccccaggt gctgcaatgg
    20041 gtgcaacaat ccggtattca aagtggtttg tttaccctct atatccagca tacctcagcc
    20101 agtttgctga tcaacgaaaa ctatgaccgg gatgtgctgg tcgacatgga agcttttttc
    20161 aatcgcctgg tacccgatgg cgatgatttg tttattcata cagtagaagg gccggacgat
    20221 atgccggcgc atgtacgtac agcattaacg caaaccagcc tgtccatccc ggtcttgcag
    20281 gggagggtgg cactggggca gtggcaaggc attttcctgt ttgaacaccg gcatttggcg
    20341 gctcgcagga gggtgctgat gcatgtcatg ggggagtaaa ccccatgttt tttgtaactc
    20401 agtaaaacac aaaaaaggag atgcgtatga agtggatcac aagtttggta gcgatattcc
    20461 tggcattggg gctgatttct tgtagccaaa aagaagatgt ccctgttccg gaaacctctc
    20521 ccgctgctga accagcggct caacaatccc ctgagcccgt aaaatcttct ggtggtggcg
    20581 gttatgagcc gacagcagat gagcgtgtgc cgggtattac gatgtcacag gaagaaatcg
    20641 ataagcagac tgcagaagcg ctcgccaata cgcctatgcc tgacgttcct ggtgagtcat
    20701 cctctgaaaa gtaatttcta gtctatccat caagggcgtg agtcaacgtc cttgtctaat
    20761 ctctcaaacg tcgaacgcat tggtttctga tgagtggttt tatttgacgg tgctccactg
    20821 gagtgggtca aaaggaatgc tgttgcggcg taattcgtaa taaacgccgt tttcggcatt
    20881 gccaccactg ttgcctacgg cggcaatggt atcgccggct ttaaccgcct ggccagaaga
    20941 tttatacaag gcctcattat taccatataa actcatgtaa ccattaccgt gatcaatcac
    21001 aatcaggtta ccaaagcctc gcatccagtc ggcaaagaca acttgcccgc cagcgataga
    21061 tttgacttcg ctcccttctt tggcgcggat aaaaatgcct ttccagctga cgcccgtgtc
    21121 ggcacgcgcg gtgccaaaac ggttaatcac gtcaccccgg acgggcaagc gtaagcggcc
    21181 gcgtaactgg gcaaagtttt ctttgctggc gctgtattca ggcaaggttt catttttggc
    21241 gatgaccgtg ctactggaag agttgctttc gctgtcttcg atattggttt gtggtgaacg
    21301 ctcaaaggtc cggcctgcag attttttctt gcgggccagt tctgcggctc gcttggctgc
    21361 cgcttctctt tcgcgtttgg ctttggccag cgcttcctgc tcacgctttt tggcctcggc
    21421 cagcaggcgt tggaacaatt gtgacaacgc ctcttcgtct tttttcaggc gcaccatctg
    21481 ttgttcttgc gtttcgatct gtttggagag ttcgttcagt gcttccgtgc gccgcgattt
    21541 ttcaccttcc agtttttgtg tggtctcgct atattggttt gccaggcgtt cggtttcgtg
    21601 cagctgttcg gtggtggccg cgcgcacacg gtctatggcc tcctggttat cttgcagtga
    21661 ctgaatctgt tgcttgtgtg cagccgtcag gtaacccagg tatttcagat tacgcgtcgt
    21721 attggcagga tcctgctgtt gtaacagcat ttgcaaggga ctgttgttgc cgtggcggta
    21781 ttgctgatta agctgttcac tcagggtgtt gcgctgttgg ttgacctggc gctgcaatag
    21841 cgataactgc ttttgcaggt cctgcaattt gccgcggttt tcctgttgtg catcctgaat
    21901 ctcacgcagt ttcttgcgtg aggcactgat cgctgtttca gacgctttca aggcttcatt
    21961 ggcatcttgt ttggcgaccc gggaggcttt tagcgcgttc gcgagctgct gtattttttc
    22021 cttgacctgc tccaggtccc ctttggtcgt ctctgccttg tcgtttgcgg cggctggtgc
    22081 gggtaggggc aatgtgccca gccagacaca caggcaaaca gtgaagcagt aggcccatcg
    22141 ggaggatggc ttggataagg cgaaaatgct gaattcagac atgcaccaag ctaaccagcc
    22201 taagccattg catccagggt gatcaggttc ttgccagtca tttctgaggg ttggggaacg
    22261 cccatgagtg acagcaacgt cggcgcaata tcagaaagcg caccgccagc agtgatgctg
    22321 gctgggcggc cgatataaat gaacggcacc agattggtag tgtgctgggt atgcgcctgg
    22381 tcgctggcgt ggtcaaacat gctttcggca ttgccgtggt cagccgtaat aatcacttct
    22441 gcccccattt tctgtgcggc tgctaccaca cggccaatac aggtatccag ggtttctacc
    22501 gctttgattg cggcttgcaa gttgccggta tggcccacca tatcaccgtt ggcgtagttg
    22561 cagatcacgg cctggtattg tcctgattca atggctgcca ccagtttgtc ggtaacttct
    22621 ggcgcactca tttcaggttg caagtcgtaa gtggcgactt tgggcgaagg caccaggata
    22681 cggtcctcac catcgaacac ggtttcttca ccaccattga agaagaatgt cacatgcgga
    22741 tatttttcag tttcagcaat acgtaactgt ttcaggccca gcttggcgac atactcgcca
    22801 aacgtattag ggacggtgta tggcgcaaaa atcggttcgg ccagcatttg atttttatcg
    22861 tattgagtca tcgtaaagta gtgactgaag tgcgggatgc ggctgcgggc aaagccggta
    22921 aattgctggt tgagcagggc gtcagtcaat tggcgggcgc ggtcgctcct gaagttcata
    22981 aacaccaggc aatccccgtc ttccatgcgt actggtgcct cctccgcggc gcggatagcc
    23041 gtgcatttga cgaactcatc actttcatca cgggcatagg cttgctgcaa ggcggttaag
    23101 ctatctggcg cgacataatc tgaggtgcca ttgaccagta attcataagc gactgacaca
    23161 cgttcccagc gcttatccct gtccataccg aagaaacgac cgccaacaga ggcaaccttg
    23221 cccacaccta aactgttaag gtggtcttcc agcgtttgca gatatggctg tgcgcttttg
    23281 ggcggggtgt cacggccgtc aagaaaagca tgcacataca ctttgctcaa gccttgctgt
    23341 ttggccatgt ccaggatggc atgaatgtgt ggctggtagc tatgcacgcc accgtcagac
    23401 aataagccca ggatatgcag cgctttatca ttggccttga gtgcctgcat ggcgttgacg
    23461 agtgcaggca actggaaaaa ctcgccactg ctaatgctgt tattaatccg ttcaaagtct
    23521 tgaaagatga tgcgtcccgc cccaatattc aggtgaccca cttcagagtt acccatctgc
    23581 ccgtctggca agccgacata gtgttcggat gcattgatca gactgtgtgg ataagtggct
    23641 ttgagcctgt cgaggttggg cgtgttggct tgcaatacgg cattaaattc ggtggtctcg
    23701 ctgtgaccaa agccatcgag aatcagcaaa atgacaggtt tgatagacat ggctaacaat
    23761 ctttaaagtg gtgacgcaac gtcgattgag gaattcgtct attataatcg caaccgcagg
    23821 cgacgcctac ccaatgggcg ccgggctttg caaacataac gtcaataaat gggtgaacca
    23881 agtggaattt tttaaacaga atgtattgtt aatcggtctg gcaattggct ctggtgtggc
    23941 tttgttgctg ccgatgctgg gccgctctgc ggcgggactg actgtgctct cagtcacaga
    24001 agcagtgatg ctcatgagcc gaaaatctgc attggtactg gatgtgcgtg aagcggatga
    24061 gtttgcacaa ggccatttac agggcgcacg taatatcccg ctctcgcagc tggagtcacg
    24121 tagcaaggag ctggaaaagt ttcgtgataa accggtgttg ctggtctgcc agcgcggtag
    24181 ccgtgccaac aaggcagcca aattattgaa gtctcaacag tttagtgcgc tgaatgtgct
    24241 gaaaggcggc atgcaggcct ggcttgaggc caagatgccg actggcaaat aaaaattaac
    24301 gttaaaggat cattatggct aaagtagtca tgtatacctc agcagtgtgc ccatactgca
    24361 tgaatgctga acgcttgctt aaaaacaaag gcgtgaccga gattgaaaaa atccgcgtcg
    24421 atttgcagcc ggagttgcgt gctgaaatga tggaaaaaac cggtcgccgc acggtgccgc
    24481 agatatttat tggcgaccag cacgtaggtg gctttgacga cctgcacgcg cttgatgtgc
    24541 aaggcgggct ggatccattg ctggccgcat aggcgcgacc gtttttgtca taaaaaacac
    24601 atagtcaggc aacacatgtc cggttagaat agcggctttg tgtattgatc aattaaggat
    24661 aattcatggc tcaggattca cagaaccaaa cagaacaaaa cgcagcgcca gtatttagca
    24721 ttgagaagct gtatgtacat gacgcctcta tcgaagtgcc gaatgctcct gcgattttca
    24781 ctgagcgtac gacaccgcaa atcaacgttg aactgggcaa cagcgcacag caaatcgaag
    24841 acggtatctt caatgtgtcc atcaaggtca ctgtgactgc taaaatcgaa gacaaaactg
    24901 cgttcctggt tgaagtaaac cagtctggca ttttcgcaat ccgcaacgtg ccacaagaaa
    24961 acatggagcc tattctggcg gtggcttgcc ctaacatcct gttcccatac gcacgtgaag
    25021 cgatttctga catggtgacg cgtgctggtt tcatgccagt gctgttgaac ccgattaact
    25081 tcgaagcgct gtacctgcaa cagcagcaac aagctgcgca agcggctggc gcacctaact
    25141 aattttgcct caagaatccg tcaccgctca ctgagccggt ggctttctgg ataaattaaa
    25201 atgagtaagg ttgcagttct cggagccggt gcctggggta ctgcgctcgc tatgcatatc
    25261 gcgttgcagc acgacgtcgt gctgtgggca cgtaatggcg ggcatgtctc cggcatgcgt
    25321 aaggcgcgcg ctaatccgct ttatcttggc gattttcctt ttcctgaaaa actgacggtc
    25381 gaagacgatc ttggtgccgc gattgctggc gccgacctga ttctgtctgt cgtgccaacg
    25441 gctggcttcc ggccaatctt gcagcaactg aaagccttgg gcattgatca gccgctgatt
    25501 tgggcaaata aaggcttgga gccgcaaacg gcaaaattgc cttttgaggt ggcacaagac
    25561 gagttgggta gtgaataccc ctggggcgtg ctcagcggcc ccagctttgc cgcagaattg
    25621 gtgcgtggct tacctactgc cattaccctg gctgccaatc atcgtgaact cgcgcagcag
    25681 gcggcacaac ttatccacgg tggctgtttg cgggtgtatg acagtgtcga tgtggtcggt
    25741 gcctccgttg gtggtgcagt gaaaaacgtg atggcgattg ctgccgggat ctcggatggc
    25801 atgggtttcg gcaacaatgc acgcgccgcc atgattacgc gtggcttggc cgaaatcaca
    25861 aggtttggta tggcactggg tgccaaggct gagacgttta tgggactggc aggcgcgggc
    25921 gacctgatat tgacctgtac cggtcaatat tcacgtaacc gtgaagttgg tttgcaattg
    25981 gcttctggca agtcactgga aagcattctt gcaggcttgg ggcatgtggc cgaaggggtg
    26041 aatactgcgc gggaagtgat gcgccgtgct gaaaccatcg gcgtagaaat gccgattacg
    26101 tttgaagtga accagttgct gaccaaccag aaaacggcgc aggaagccgt gacggcctta
    26161 ttgggcagag gccagcgcca agag
//
LOCUS       DATMSK010000270         6252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_92357,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000270 DATMSK010000000
VERSION     DATMSK010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92357"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3113
                     /locus_tag="VNZ84_09005"
     CDS             <1..3113
                     /locus_tag="VNZ84_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU67137.1"
                     /translation="WVIAIVIMLAGIGAIEWLPLEQYPDIAPPRVSINANYTGAAADT
                     IENSVTQVIEQYMKGIDNLTYMSASSTSAGTANISLTFDAGTNPDVAQMQVQNKLQQA
                     MPRLPQIVQSQGVTVAKTGIDFLMMISLMSDSPTVRAIDIGDYISSNLIDQISRLDGV
                     GDVQSLSSGYAMRIWLDPAKLQKYNLIPSDISAAIQAQNTEVSSGQLGALPAVKGQGL
                     NAPITARSKLQTVDQFKQIIVKSGADGYVVHLSDVARVELGAESYTPVANLNGKTSAA
                     FGVKLASGANALATAQAVKDKLAALEPYFPPELKLSIHIPYDTTPFVRISIEEVVKSL
                     IEAILLVVIIMYVFLQNLRATLIPAIAVPVVLLGTFGVLAAFGFSINTLTMFGLVLAI
                     GLLVDDAIVVVENVERVMREEGLSPKAATRKSMEEITSALVGISLVLSAVFIPMAFFG
                     GATGVIYRQFSITIVSAMLLSVLVALTLTPALCATMLTPAHGDHAPTGGLFAWFFRWF
                     NPAFQAFANGYQSVVEKILRQRLFTMVIYAGIIAVLSVLFMRLPSSFLPTEDQGVLMT
                     QIQLPVGATDSRMQRVLRQIEQYLDSQHAVIDEYLTIAGLGQGGGGSQNTGRAFIRLK
                     DWSLRGEGEDAASLADKMTKALSKIRDAKLFVNQPSPVRGLGQGSGFTLELKDVGGLG
                     HQGLMEARNKFVQLAADNPKLNRVRPSGLDDTPMFRLNIDDQKTGIYNLTTANINSTL
                     SIAMGGTYVNDFINKGRVKKVYLQGDAPYRMQPEDINRWYVRNTIDEMVPFSAFSNTR
                     WVSGSPLLERYNGISSMEIVGEAAKGTSTGEAMRIAEEIIKQLPAGIGYEWSAQSYQE
                     RLSGSQAPLLYAVSVVFVFLCLAALYESWAVPFSVILVVPLGILGAVLATSLRGMTAD
                     VYFQVGLLTTIGLSAKNAILIVEFAKHLQERGRPLLEATLEAVKLRLRPILMTSLAFV
                     FGVLPLAFSHGAGAVSRQAIGTGVLGGMLTATFLGVFFVPLFYVLIRSLFPYQPEADS
                     VSPAHD"
     gene            3106..4569
                     /locus_tag="VNZ84_09010"
     CDS             3106..4569
                     /locus_tag="VNZ84_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWU67138.1"
                     /translation="MIKVIMTLNPRHLAGLLILTLSLSGCIDLSPFYTRPAAPVPATL
                     GNAQGDGQKALTDLPWQSFISDDKLRQVVDLALKNNRDLKVAALTIEQSRALYQVQRA
                     DLFPTIAATVSETASKGLSNSGNVGAATTGTAGTVASSNNQSGKVSHIYRATVGFSAY
                     ELDLFGRIRNLNQQALQTFYATEENRKSTQISLIAEVSTAWLTLAADKKRLAIAQQTL
                     QSQQATFNINQKMFELGVANALTLKQLQTSVDTARVAVATYTMQIKQDMNALNLLAGG
                     IVPEELLPSAVLANVVSQPLPAGIRSDILQQRPDVLAAEHQLQAADANIGALRAAFFP
                     TITLTSTYGSASTELSGLFAKNSNIWSFVPQISIPIFNAGRNRANLAAGEKNRDILLA
                     QYEKTVQTAFREVADVLAQREGLQSQLEAQQSLTEAAAEAFKLADARFKNGVDSYLVV
                     LDAQRTLYTAEQSLVTLQLSDAASQLTLYKVMGGGWH"
     gene            4809..6080
                     /locus_tag="VNZ84_09015"
     CDS             4809..6080
                     /locus_tag="VNZ84_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HWU67139.1"
                     /translation="MQTEISSEHTIILAKRRHIEELERLIICTEFVELIGEMIHQIQA
                     ERGASCLYLASGGQRFAKERADIVAGNAPLDMRFKQGLQRHLDANPQADAKQLTLVSW
                     ILLGFDQLTVLRHQVTLQQISFIDCIQAFTRLIGSLVSLIFEITDTTVNSKISSSLLA
                     LYNLVQGKEFAGQERAVGAHMFGSGNVQIEHQQKVMELMALQERHFESFNQFATPALR
                     NIWVRTSMTDTQRKFLQYQSKLTSASPGQTLQPKDGDCWFELCSQRLTDFWQIQCLLI
                     SDMHQALKLLLQEAQQDLKNTRQHLQQLRDKAKVPGRDSAFFNLSIPVEHSYGFLTDK
                     ANQPYPIESVISLLQQQSQQIAEMESELSETKKALTERKQIERAKGLLMSTFGLTEVE
                     AYQSMRSTAMEQKRKIIDVAENILFQHKQAS"
BASE COUNT         1539 a         1719 c         1580 g         1414 t
ORIGIN      
        1 cctgggtgat tgcgattgtc atcatgctgg ccggcatagg tgcgattgaa tggctcccgc
       61 tagagcaata ccccgatatt gccccacccc gcgtctccat caatgccaac tacaccggcg
      121 ctgcagctga caccattgaa aactcggtga cgcaggtgat tgagcagtac atgaagggca
      181 ttgataacct gacttatatg tctgccagca gtacatctgc cggcacggcg aatatcagcc
      241 tgacttttga cgccggcacc aacccggatg tggcgcagat gcaagtccag aacaaattgc
      301 agcaagccat gcctcgcctg ccgcagattg tacaaagcca gggggtgaca gtcgccaaaa
      361 ccggcattga ctttctgatg atgatttcac tgatgtctga cagcccaacg gtacgggcga
      421 ttgatatcgg ggactatatt tccagcaacc tgatcgacca gatcagccgc ctggatggcg
      481 tgggcgatgt acagtcgctg agttcaggct acgccatgcg tatctggctg gatccggcca
      541 aactgcaaaa atacaacctg attccttccg atatttctgc ggcgatccag gcacagaata
      601 cggaggtctc ctctggccag ctgggagcgc tgcccgctgt caaaggacaa ggcttgaatg
      661 cgccaattac cgcacgcagc aaattgcaaa cggtagacca gtttaagcaa atcatcgtca
      721 aatccggtgc agatggctat gtggtgcatt tgtcggatgt cgcccgggtg gaactgggcg
      781 cagagagcta tacaccggtc gcgaacctta acggtaaaac atcggcggca ttcggtgtca
      841 aactcgcttc aggcgccaat gccttagcca ccgcacaggc agtcaaagac aaactggcag
      901 ccctggaacc atactttccc cctgagttaa aactcagtat ccatatccca tatgacacca
      961 ccccgtttgt gcgcatttcc attgaagagg tcgtcaaatc actgattgag gcgattttgc
     1021 ttgtggtcat catcatgtat gtgtttttgc aaaacctgcg tgccaccctg attccggcca
     1081 ttgcagtgcc cgtggtcttg ctgggcacat ttggcgtgct ggctgccttt ggcttttcca
     1141 ttaacacgtt gaccatgttt ggcctggtac tggccattgg cctgctggta gatgatgcca
     1201 ttgtagtggt cgaaaacgtc gagcgcgtca tgcgggagga aggcctgagc ccgaaagcag
     1261 ccacccgcaa atccatggaa gaaatcacca gtgccctggt tggcatttcc ctggtgctgt
     1321 ctgcagtatt tattcccatg gccttctttg gtggcgccac aggggtgatt taccgccagt
     1381 tctccatcac cattgtgtcc gccatgctac tctcagtgct ggttgcactc acgctaaccc
     1441 ctgcgttatg tgccaccatg ctgacaccgg cacacggcga ccatgccccg acaggcgggc
     1501 tattcgcctg gtttttccgc tggttcaacc cggctttcca ggcatttgcc aatggctacc
     1561 aatccgttgt cgaaaaaatc ctgcgccaac gcctgttcac catggtcatc tatgccggca
     1621 ttattgccgt actcagcgta ttgtttatgc gtttacccag ctctttcctg ccgacggaag
     1681 accagggtgt gctgatgaca cagattcaat taccggtagg cgcaacggac agcagaatgc
     1741 agcgcgtgct taggcagatt gaacagtacc tggacagcca gcatgccgtg attgatgaat
     1801 acctgaccat tgctggcctt ggccagggtg gtggaggtag ccaaaacacc ggccgtgcgt
     1861 ttatccgcct caaggactgg tcactccgtg gcgaaggtga ggacgcggca tcgctcgcgg
     1921 acaagatgac caaagcctta tccaagatcc gcgatgccaa actattcgtc aaccagccct
     1981 ccccggtgcg tggactgggc caaggttccg gctttacact ggaactcaag gatgttggcg
     2041 gtttggggca tcagggactg atggaggcca ggaacaaatt tgtacaactg gccgcagaca
     2101 atcccaagct gaaccgggta cggccgagcg ggctggatga tacgccgatg ttcaggctta
     2161 atattgatga ccagaaaacc ggcatttata acctgaccac agccaatatc aactccacgc
     2221 tgtctattgc catgggtggc acttacgtga atgactttat caataaaggc cgtgtcaaga
     2281 aggtgtatct gcaaggggat gcaccttacc gcatgcaacc ggaagacatc aatcgctggt
     2341 atgtgcgcaa cactatagac gaaatggtgc ccttctccgc gttttccaat acacgctggg
     2401 tgtccggctc gccactgcta gagcgttata acggaatttc ctccatggaa atcgtcggcg
     2461 aagctgccaa gggcaccagt acgggggaag ccatgcgcat tgccgaagag attatcaagc
     2521 aattaccggc tggcattggt tatgagtgga gcgcccaatc ctaccaggaa cggttgtcgg
     2581 gctcgcaagc accattgctg tatgcggtct ccgtcgtctt tgtgttcttg tgcctggccg
     2641 ccttatatga aagctgggcg gtgccatttt cagtcattct ggtcgtgcca ttaggtatcc
     2701 tcggcgcggt gctggcgacg agtttgcgcg gcatgacggc agacgtttac ttccaggtgg
     2761 gactgttaac caccatcggc ctgtctgcca agaatgcgat cctgatcgtg gaatttgcca
     2821 aacatctgca agaacgtggc aggcccttgc tggaggctac actggaggca gtgaagctgc
     2881 gcctgcggcc tatcctcatg acctcgctcg ctttcgtgtt tggcgtactg ccgctggcgt
     2941 ttagccatgg cgcaggcgcg gtcagccgtc aggccattgg caccggggtg ctcggtggca
     3001 tgttgacggc gacctttctc ggcgtatttt ttgtgccatt attttatgtg ctgatacgtt
     3061 cgctattccc ttaccaacca gaagcagatt ccgtaagccc ggcacatgat taaagtgata
     3121 atgacattga atcccagaca cttggcaggc ttgctgatac tgacactcag cctgagtgga
     3181 tgcatagacc tgtcgccctt ctacacaagg ccagcagcgc cggtgcctgc caccttgggc
     3241 aatgcacaag gagatggcca aaaagcactc accgatctgc catggcaatc atttattagt
     3301 gatgacaaac tgcgccaggt cgttgatctg gcgctcaaaa acaaccgtga cctgaaagtg
     3361 gcagcgctga cgattgagca atctcgtgcg ttataccagg tccagcgtgc cgatctcttc
     3421 cctactatcg cagccaccgt ttcggaaacc gcctccaaag gattatcaaa ttcaggtaat
     3481 gttggggcgg ccaccacagg cacagcgggc acggtcgctt ccagcaataa ccagagtggt
     3541 aaggtctcac atatttatcg cgcaacagtt ggatttagtg cttacgaact agacctgttc
     3601 ggccgcatcc gcaaccttaa ccagcaagcc ttgcaaacgt tttatgccac cgaggaaaac
     3661 cgtaaaagca cccagatcag cctgattgca gaagtctcta ccgcctggtt aaccctggct
     3721 gcagacaaga aaagactggc gattgcacaa caaaccctgc aaagtcagca ggcgactttc
     3781 aacatcaacc agaaaatgtt tgagcttggt gtggcaaatg cgttgacctt aaaacaattg
     3841 caaaccagcg tggacactgc ccgtgtcgct gtcgccacct ataccatgca aatcaagcag
     3901 gacatgaatg cattaaacct cctggcaggc ggcatcgtac ctgaagaatt attgccgtcg
     3961 gcggtactgg cgaatgtcgt ctcacagccg ctgcctgcgg gcatccgctc ggacattttg
     4021 caacaacggc cggatgtgct tgccgcagaa caccagttac aagctgcgga tgccaatatt
     4081 ggcgccttgc gggccgcttt ttttccgacc atcaccctga ccagtacata cggctcagcc
     4141 agtaccgagc tctctggact gtttgccaag aactccaaca tttggagctt tgtaccgcaa
     4201 atcagcatcc ccatattcaa tgccggccgc aaccgtgcca acctggccgc aggtgaaaag
     4261 aaccgtgaca tcctgctggc acaatacgaa aaaaccgtac agaccgcatt ccgcgaagtt
     4321 gccgatgttc tggcgcaacg cgaaggcttg caatcacagc tggaagcaca acaatccctg
     4381 acagaagccg ctgcagaggc cttcaaactg gcggatgccc gctttaaaaa tggcgtggac
     4441 agctacctgg tcgtattgga tgcccaacgc acgctttaca ctgccgagca atcactggtt
     4501 accttacagc tgagcgacgc cgccagccag ctgaccttat ataaggtcat gggtggcgga
     4561 tggcattgat gcacaggaca tgcacaatca ccattaaaaa aacgccttaa gacgtgcggc
     4621 gatgacttcg ttggtaagtc atcgcttact gtctgcgcca aggcttaagc caaagcttga
     4681 cctggcttca ccacacaact catttctcct cgcctgcggc ccataaaagt aatcaccgca
     4741 aagcaatgac cgctgtataa tcggcccccg atttataatc gcgcacattt tttgcttcct
     4801 ttttgtaaat gcagacagaa atatcttccg aacacaccat tatcctggcc aagagacggc
     4861 atattgagga gctggaacga ctcattattt gcactgaatt tgttgagctg ataggcgaaa
     4921 tgattcacca gattcaggct gaacgagggg ccagctgcct ctacctggct tcaggcggcc
     4981 aacgctttgc caaagagcgg gccgacatcg tggcaggcaa tgcaccgctg gatatgagat
     5041 tcaagcaagg attacaacgc cacctggatg ccaatcccca agccgatgcc aagcaactca
     5101 cgctggtttc atggatatta ttgggctttg accaattgac ggtgttgcgg caccaggtca
     5161 ctttgcagca aatatcgttt atcgactgta tacaggcgtt tacccgcctg attggcagcc
     5221 tggtatcgct gatttttgag atcacggaca ccacggtcaa cagcaagata tccagcagct
     5281 tgctagcgct gtataacctg gtgcagggca aagagtttgc cggccaggaa cgtgccgtgg
     5341 gtgcacatat gtttggctct ggtaatgtgc agatagagca ccagcagaaa gtcatggagt
     5401 taatggccct gcaggaacgc cattttgaat cgtttaacca gttcgcgaca ccagctttac
     5461 gcaatatctg ggtgcgaacc agtatgaccg atacgcaacg caaattcctg caataccaaa
     5521 gcaagcttac cagtgcctcg cctggccaaa ccttgcaacc caaagatggt gactgctggt
     5581 ttgagctttg cagccaacgc ttgactgact tttggcagat tcagtgcctg ttgatcagcg
     5641 atatgcacca ggccctcaaa ctactgttac aggaggcaca acaagacctg aaaaacacac
     5701 gccagcattt gcagcaatta cgggacaaag ccaaggtgcc agggcgcgac agcgcatttt
     5761 tcaacctgtc gatacccgtt gaacattcgt atggcttttt aaccgacaaa gccaatcaac
     5821 cctatcccat agaatctgtg atctccttat tgcaacagca gtcacaacag atcgcggaaa
     5881 tggaatccga gctctcggaa accaaaaaag cactcacaga aagaaagcag atagaacgcg
     5941 ccaaaggctt gctgatgagc acattcggcc tgacagaagt ggaagcttat caatccatgc
     6001 gcagtactgc catggagcaa aaacgcaaga tcattgatgt ggcagaaaat atcctgttcc
     6061 aacataaaca agcatcctga ttgccctatg gacgtggtgc atccgcaccc ctatggtgcg
     6121 ccactcttat gtatagcccc tcttcatcca tcgacaggtg aaataaaaac accttaaaaa
     6181 ttaagcagtt aaatatcaca cccaatttgg catgccactt gctaatgctc ctttgagtgg
     6241 gcatcaatgg cg
//
LOCUS       DATMSK010000271        22212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_117279,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000271 DATMSK010000000
VERSION     DATMSK010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 22212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22212
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117279"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1896)
                     /gene="recQ"
                     /locus_tag="VNZ84_09020"
     CDS             complement(70..1896)
                     /gene="recQ"
                     /locus_tag="VNZ84_09020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HWU67140.1"
                     /translation="MASPSPQQILQEVFGYNQFRHQQQAIVEHVIAGDDALVLMPTGG
                     GKSLCYQIPALARDGLAIVVSPLIALMQDQVEALQQLGVNAAFLNSSLSAEDNARITR
                     QVVSGEIKILYVAPERLMVGSFLSLLDEVQQHVGLALFAIDEAHCVSQWGHDFRPEYR
                     QLTVLHNRFPEVPRIALTATADAPTRAEIISQLNLEYARQFVSSFDRPNIRYHVGIKN
                     NARQQLQAFLEREHPDDAGIIYCLSRRKVEETAAWLVEKGWSALPYHAGLPAQLREHH
                     QRRFLREEGIIMVATIAFGMGIDKPNVRFVAHLDLPKSMEGYYQETGRAGRDGLQANA
                     WMVYGMGDVVSMRQMLDSGEASEERKRLERLKLDALLGYCESTACRHQSILRYFGESH
                     PGACGQCDNCLHPVETWQATQVAQMALSCVYRTGQRFGVGHLIDVLMGKVTPQVERFG
                     HDKVSTFGIGKTLNQTQWSGVYRQLIAAGLLETDLSGYGGLHLTEAARPVLKGEQEVW
                     LRRDAEPEKRMSKAERSARAKEAFEGANDDPLWQALKAKRLELAREQGVPPYVIFHDS
                     TLLEIHNRKPQTLDEMGRISGIGQAKLQKYGDAFLQVFEGMI"
     gene            complement(1910..2125)
                     /locus_tag="VNZ84_09025"
     CDS             complement(1910..2125)
                     /locus_tag="VNZ84_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67141.1"
                     /translation="MSNEWPKWVRDDGSVVSCTEKVKVMAENFDELKQIAQDALEDGL
                     LMEVSEEQMRKALHELVDQLINPYNKI"
     gene            complement(2146..2769)
                     /gene="rdgB"
                     /locus_tag="VNZ84_09030"
     CDS             complement(2146..2769)
                     /gene="rdgB"
                     /locus_tag="VNZ84_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HWU67142.1"
                     /translation="MALPFNKLVIASGNKGKIREIAHLLAPLNIEVVPQSELHVPECP
                     EPHCTFIENALAKARHASQHTGLPALADDSGLCVDALQGAPGVISARYAGAQPTKSLL
                     TQDERNNDKLLTAMERIADRRAYFYSVVVLVRNPQDPQPIIADGMWRGEILREFRGTE
                     GFGYDPLFLDTQTGKTVAELPLEIKSQLSHRGQAMAKLLQQLHTLSN"
     gene            complement(2772..3491)
                     /gene="rph"
                     /locus_tag="VNZ84_09035"
     CDS             complement(2772..3491)
                     /gene="rph"
                     /locus_tag="VNZ84_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWU67143.1"
                     /translation="MSRPSQRANDQLRAIQIIRHYTKHAEGSVLVKFGDTHVICTASV
                     EEKVPGFLKGKNQGWVTAEYGMLPRSTGSRMDREAARGKQTGRTQEIQRLIGRSLRAV
                     IDMEKLGERTIHFDCDVIQADGGTRTASITGAYVAMVDAVSTLLQKGLLSESPIKDSV
                     AAISVGVYQGTPVLDLDYIEDSDCDTDMNVVMTGKGGFVEIQGTAEGEPFARETMNQI
                     LDLAAKGISEISVIQKTALAA"
     gene            complement(3507..4424)
                     /locus_tag="VNZ84_09040"
     CDS             complement(3507..4424)
                     /locus_tag="VNZ84_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HWU67144.1"
                     /translation="MKFTIAQGSRQGPRPYNQDRLAYSYHRHAILMVLADGMGGHQHG
                     DVAAEVAVKTLVEAFQRESKPLIVQPAEFLRTQIVQAHEVIENVRMQKRLMESPRTTI
                     VVALIQHNKLFCAHVGDSRLYHYRNGLVEHQTEDHSVVQSLLREGKITAAEVDHHPHR
                     NKIYNCLGGERAPKVELTPPIPLREGDVIMLCSDGVWNTVPEMNMAQHMLAADINDGV
                     SRLLDDADAASITVGDNMSAIALQWGQRVVSPLTISTLDLPVDVTTTMMNESPATPSP
                     YGLDLTDDEIEKAIAEIQEALGRTQQHIK"
     gene            complement(4421..5356)
                     /locus_tag="VNZ84_09045"
     CDS             complement(4421..5356)
                     /locus_tag="VNZ84_09045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWU67145.1"
                     /translation="MKLSNKFDFALPVGTALHDYTITRVLSLGGFSFVYLAQDPQKNT
                     VAIKEYMPVTLAERDEHTHIRSNGKNEAAYKHGMKCFFEEGLALANIEHKNIVRVKNF
                     FRANNTVYMVMKYERGKSLQDYIMALSQPVPENFLIRMFIELLNGLREVHTRKLLHLD
                     IKPANIYIRLDGSPMLLDFGSARQALSEVTLAPTYTPGFAPPEQYIDRKHLGPWSDIY
                     SIGASMYACLQRSSPVSANQRLKEDTLVPAVELGKQIYSEKLLAIIDECMQLDYLSRP
                     QSVFSLQKKMIGIHVAENPRGSLVDKIIKVLNKPL"
     gene            5537..6427
                     /locus_tag="VNZ84_09050"
     CDS             5537..6427
                     /locus_tag="VNZ84_09050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HWU67146.1"
                     /translation="MTAKKTAQDYPILSMTGFAATESRFPGGLLLIELRAVNHRYLEL
                     NVKIDDSLRQYEAHIREQLQSRLGRGKVECRMSLKSDHAATHLALNPAMVAQISQTLS
                     ELQQHFPQAQPVNLVEVSKLPGVLQSEAVDAEALAQGLLNGLSHAIDELIAARQREGE
                     KLKQVLLNRLQGVREQISIVKPLLPALVSQYQEKLTAKLREAMQADDDRVRQEIVLYA
                     QRIDVDEELARLQSHIVEMDRILSAGGAVGKKLDFLMQEMNREANTLGSKSVAIETTQ
                     VSMQLKVLIEQMREQIQNIE"
     gene            6588..7040
                     /locus_tag="VNZ84_09055"
     CDS             6588..7040
                     /locus_tag="VNZ84_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67147.1"
                     /translation="MAETTMLVIPDQGWSVTFDAPPLRPVREADSNDHYMYSANANNF
                     AVTLYVDTPPCNGDDSHEAALNCYWAKAGKDPLINQASVTKNCNQQYCKISYDFENSF
                     QGASIKQKHVHFVFTHRDRWSNLHAAVSNPSENDLKLLEAFTQSFSYQ"
     gene            7230..8723
                     /locus_tag="VNZ84_09060"
     CDS             7230..8723
                     /locus_tag="VNZ84_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWU67148.1"
                     /translation="MTQSAKQFFDEMQGYGGNVRAIYQAYKHWLSDVPTQQLAAKRAE
                     AEVLFRRVGITFNVYGEEDGAERLIPFDVIPRMISASEWAHLSKGAVQRVRALNMFLH
                     DIYHDQEIIKAGIVPHSILSNAQYRPEMVGVNVPNQVYAHVAGIDLVRTSESQFYVLE
                     DNLRTPSGVSYMLEDRKMMMRLFPDLFKKYPIAPVEHYPHVLLNNLRSVAQTGVTDPT
                     VVLLSPGAYNSAYFEHAFLAQQMGIELVEGQDLFVRNNAVYMRTTQGPQRVDVIYRRI
                     DDDFLDPLVFKPDSLLGVPGLLSVYRNGGVTLANAVGTGVADDKATYIYVPEMIKFYL
                     GESPILQNVPTYQLSKEDDLAYVLDHLPELVVKEVQGSGGYGMLVGPTASKQEIEEFR
                     LRIVSKPSNYIAQPTLALSTCPTLVDQGIAPRHVDLRPFVLSGKTVSVVPGGLSRVAL
                     KEGSLVVNSSQGGGTKDTWVLERDDVCVDTAQSQMAAQQMNQEAVIC"
     gene            8717..9661
                     /locus_tag="VNZ84_09065"
     CDS             8717..9661
                     /locus_tag="VNZ84_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWU67149.1"
                     /translation="MLSRTADHLYWMARYIERTENVARVLDVTYNMSLLPSDSSNEEE
                     LWDTVLEIAGIRDIYDATYKELNAANVIKHLVMDSHNPSSIYSSLRSARENARAVRVA
                     MTTETWENMNTLWLEFGQYIKQDLTKTGLSEFCEWVKSRSHLFRGVCFGTMLRDEAFS
                     FVRLGTFMERADNTARLLDVKYEFLIPMQEANDGAVDYYEWSAVLRSVSAFQAYQKVY
                     RDAIEPMKVSELLILREDMPRSLHACYAEIMPILRQVAGSRQTEAERLAGQMHAKLHY
                     GRMLDIVRDGVHHFLDEFILANRKLGEEIQRNFLSATA"
     gene            9766..10593
                     /locus_tag="VNZ84_09070"
     CDS             9766..10593
                     /locus_tag="VNZ84_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWU67150.1"
                     /translation="MLLNIEHQTQYVFSEVVQYTIQQLRLTPRNGYGQAVKHWQIKMN
                     GQLTPFEDAFGNLSHTLVIDQPHDSLLITVSGAVETGLQTEQPEQALPPEIYLRDTPL
                     TQADAAMLEFARPFANAPLTALMQALRERMQYIKGATQVDTPAAEAFRLGKGVCQDHA
                     HAFIGCCRSLGLPARYVSGYLFTVDGSLMQTHAWADVWQQGQWLGLDVSNGTLVNETH
                     VRLAHGLDYRSASPVTGSRMGGGIEGMASVVSVNQQESRAKPQDQARLLQQAQSAQQ"
     gene            10651..11388
                     /locus_tag="VNZ84_09075"
     CDS             10651..11388
                     /locus_tag="VNZ84_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HWU67151.1"
                     /translation="MTYCVGILLEQGLVLASDTRTNAGVDQVAIAPKMHVFEVPGERI
                     IVLLTAGNLAITQSVVNLCREQLKSPEPHAHLHNVQSLFEAASVVGAAMRSVHQRDGK
                     ALKEHSIDFSASILVAGQIKGEKPRLFNVYAAGNFIEATPDTPYLQIGETKYGKPIID
                     RVVKHQTNVTDAVKCVLISFDSTIRSNISVALPIDLMIYRTDSLNADCRQRLMEDDPY
                     YASIRKGWSEGLKAVFSSLPNPAWSTS"
     gene            11385..11996
                     /gene="gmk"
                     /locus_tag="VNZ84_09080"
     CDS             11385..11996
                     /gene="gmk"
                     /locus_tag="VNZ84_09080"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWU67152.1"
                     /translation="MSHGHLFVISAASGAGKTSLVRAMLAQDPILKLSVSHTTRAPRP
                     GEEDGIHYHFVSEPEFLQILDSGGFLESAHVHSARYGTAQSEVENALAAGRDVILEID
                     WQGAQQIRTLFPQVTSIFILPPSIEALSHRLNSRGQDSEAVITQRLAAAREEMRHVHE
                     FDYVTINDNFDVALQDLMAIVRAQRLQLNRQVACHASLIQSLT"
     gene            12043..12258
                     /gene="rpoZ"
                     /locus_tag="VNZ84_09085"
     CDS             12043..12258
                     /gene="rpoZ"
                     /locus_tag="VNZ84_09085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWU67153.1"
                     /translation="MARITVDDCLDNIPNRFQLTLVAAYRARQLANGSDPLVSGYSQK
                     DKATVLALREIAAGKIGVELLEKKGQA"
     gene            12265..14481
                     /locus_tag="VNZ84_09090"
     CDS             12265..14481
                     /locus_tag="VNZ84_09090"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWU67154.1"
                     /translation="MPHTQGDSAHRKSRPPLEEVDLLPADREDSPLTQLIRKYLNEEE
                     VQLVWQAYRYADQAHAGQTRKTGEPYITHPISVACILARLHLDLPTLLAALLHDVVED
                     TEVDSAAITERFGKQVADLVDGLTKLDKVELQTATQAQAENFRKMLLAMSQDVRVILV
                     KLADRLHNMQTLDAMRAEKQKRIARETLDIYAPIANRLGLNPIYHELEDLSFKYLYPN
                     RYRVIDKAIRTARGHRKEVVSKIQDAIQNQLTQYQIEYDVQGREKHLYSIYKKMSEKA
                     MKLSQIGDIYGFRVIVKDTAACYLALGALHALYKPIPGRFKDYIAIPKANGYQSLHTT
                     LFGPFGTPIEVQIRSQEMHNIADAGVAAHWLYKTTDAHLTKLQQQTHQWLQRLVEIQT
                     ESDDSHEFLENFKVDLFPDEVYVFTPKGNIMALPRGSTAVDFAYAVHSDVGNSCVAVK
                     INNELAPLRTEMRNGDHVEIITAPLAKPNPAWLNYVVTGKARSHIRHYLKTVKVEESA
                     QLGERMLNVALRAMHIEPAALSDKQWQKVMNDYGVKTRQAILTDIGLGKRNSLMVAHQ
                     LVAHPAEAEEKSAKVLDTITIRGSEGMAVQFAQCCRPIPGDPILGFINKDKGLVIHTH
                     DCPAIRKFKLDPEKWLDVEWDADPKKLFSVNLRVTVVDQRGMLAKIAATISNADANID
                     NVSVETSDGSQYSNVNFTVQVHNRVHLATLIRNLRKIQQIVRINRVKGNQLEQRIQ"
     gene            14497..16563
                     /gene="recG"
                     /locus_tag="VNZ84_09095"
     CDS             14497..16563
                     /gene="recG"
                     /locus_tag="VNZ84_09095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWU67155.1"
                     /translation="MRPLSDIKGFTPALCSQLDKLGIRSVESLLLHLPLRYIDETRIT
                     AIRDLRLGEQTQIEGVVVHQEVSYKPRKTLNVRVQDDTGVLNLRFLHFYPSQVSAMQT
                     GQRLRVFGEVRPGFFGYEMVHPTQRKVKEDTPVNSALTPVYPTTAGLSQSAIRKAVKL
                     VLHGEHLHETLPDWVYQGMRLPDFASSLRLLHQPSPETALNTLEDRTHPAWQRLAYDE
                     LLAQQLSMRQHYARRKQSQAPAISGSQHLVARLLASLPFALTSAQQKVVHEIQHDLAQ
                     PHPMQRLLQGDVGSGKTIVACIAALQLIEHGWQVAIMAPTEILAEQHYKKLCGWLQPL
                     GITVAWLTGSQPKKEREQSLSLIANGDAMLAVGTHALFQDDVEFKQLGLAIVDEQHRF
                     GVGQRLALRKKGTGKNDTVSRWEPHQLMMSATPIPRTLSMSYYADLDVSVIDELPPGR
                     TPVITKLVADDRREEILQRVHDACAQGHQAYWVCPLIEESEALQLQTANDTFAYMQQA
                     FPELRVGLVHGRMKTAEKQTVMQAFAANDIHLLVATTVIEVGVDVPNASLMVIEHAER
                     MGLSQLHQLRGRVGRGSAKSTCILLYNKQQCSQIGRQRLKVIFESSDGFEIAQADLHI
                     RGPGELLGTRQSGVPMLKIADLERDADLLDIAKTMADQLLQRAPAAVSAHLQRWLSQA
                     EDWVKV"
     gene            complement(16578..18557)
                     /locus_tag="VNZ84_09100"
     CDS             complement(16578..18557)
                     /locus_tag="VNZ84_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWU67156.1"
                     /translation="MNKTAESSALQSEIVGMLYANNRRSLLAAIVVMIALLLVQRPLI
                     NPVILTIWSAVFIAAYGTRGLLTYQYEKDQQHARHSRQWLQWFRASTGFCGIAWGLAG
                     ILLFPESDTAHQAFLIFALVGVSGAGIVIYSVDTFSSNLFTGGILLFMIPRFLIHGSS
                     VSLALAVLFIVYVVYVTVAGRGLALSLRENISLRIASNLDNQKVHQLAYYDFLTGLPN
                     RRLLADRLSQAFARCVRNQTYGAVLSLDLNNFKGLNDSKGHQAGDELLQQVAARLQEC
                     MRKKDTIARMGGDEFIAVLDELSTDKAEAAAAAQVAAEKMMAVFEQPFQLQNTRYRTE
                     PSIGICLFLGDQCSQAEVLMRADIAMYQAKKSDNLNSIQMYDEATHPAIEMRATLENE
                     LAFALQANQFLLYYQIQVDAEQQPIGAEVLLRWQHLTLGMIPPDQFIPIAEDTGEIVA
                     IGQWVLAQACQQLQQWSKSALTASLQLSVNVSAIQFSQPEFVATVTETVQASGCDARR
                     LRLELTESLIVKNLDEVIGKISALRALGISFSLDDFGIGQSSLLVLKRLPLDELKIDR
                     SFIKDIVLNSYDAFIVQTIINMGKNLGHSVIAEGVEMQQQMTLLQEMGCHCFQGYLFS
                     RPLPLQDFEANLLAAMSRTKKSSAREKTSALPGQA"
     gene            complement(18704..19282)
                     /locus_tag="VNZ84_09105"
     CDS             complement(18704..19282)
                     /locus_tag="VNZ84_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWU67157.1"
                     /translation="MTNNAALIPETKGASVVITHHVSPALQAEYETWLQEIGPVCRSS
                     EGLLDWHIIRPIAGYTDTYSVMIRYETERHLTQWLDSPQRQALIAKAAHLLAGQEEYR
                     VSSGLDFLFMQPNVAPAKPPVRWKQFLVTWSAIFPLVSVVPWLFVPLLDEAGLPPNRI
                     VHTFFVTAVVVALMVYVVMPRYVKLVQHWLHR"
     gene            complement(19316..20206)
                     /locus_tag="VNZ84_09110"
     CDS             complement(19316..20206)
                     /locus_tag="VNZ84_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU67158.1"
                     /translation="MDFNEAAVFVRVVQAGSFSAAARQLGLPTSTISTRVARLEKRLG
                     VTLLQRTTRKLNLTEAGTLYFEHASTGLGYLLEAEAALDEARQQPQGVLKVTAPADLG
                     DVLLGSLIAQMQQTYPALSLELMLTQSYVDLVAEGVDVAIRTGELRDSSLIAKSLGTI
                     QWALFASPAYLQEAGSIATPTDLAQHQCVQFTSMGRHHWELQRGTTAIRVPLAARTLV
                     NSIGVVRAMAESGQGVALLPTFICQPTVQAGGLQRVLPEWRGQADAVHLVYPKQRFMP
                     PKLRGFIDLAVAQLKPLFHS"
     gene            20336..21196
                     /locus_tag="VNZ84_09115"
     CDS             20336..21196
                     /locus_tag="VNZ84_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWU67159.1"
                     /translation="MKKLAFIKRSNGNHWVGDGFPVQSIFSYRDIANDISPFLLMDYA
                     GPATFEPTTERRGVGQHPHRGFETVTIVYDGQVSHHDSTNAGGTIGPGEVQWMTAGKG
                     IIHEEYHGDDYARTGGPFEMIQLWVNLPAKDKMTAPGYQGITKAEIPEVALPDDAGKV
                     RVIAGEYAGQKGPASTFSPMNVWDVRLNAGTRTTFTLPEGHTTAVFVLHGAVKTGEVH
                     TIRPAELAVMQREGNELVLQAQQDTVLLLLNGEPLNEPVVGHGPFVMNTWEEIDQAIN
                     DYNQGRFATA"
     gene            21442..22068
                     /gene="ycaC"
                     /locus_tag="VNZ84_09120"
     CDS             21442..22068
                     /gene="ycaC"
                     /locus_tag="VNZ84_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HWU67160.1"
                     /translation="MKNKPYVRLDRDNAVVLLVDHQAGLLSLVRDIEPDRFKNNVLAL
                     ADAAKFFNLPTILTTSFESGPNGPLMPELKALFPDAPYIARPGQINAWDNEDFVKAIK
                     ATGKKQLIIAGVVTEVCVAFPALAAIEEGFDVFVVTDASGTFNEITRDAAWDRMSAAG
                     AQLMSWFGVACELHRDWRRDIEGLGTLCANHIPDYRNLMTAFNALQGK"
BASE COUNT         5350 a         6046 c         5691 g         5125 t
ORIGIN      
        1 cagaaggcca tccaatttaa cgttgttaaa gaatgattgc cagcttttta taaagaataa
       61 ggcacgtttt caaatcattc cttcaaatac ttgcaaaaaa gcatcaccat atttctgcaa
      121 ttttgcctgt cctatcccgc taatccgacc catttcatcc aaggtctgcg gtttgcgatt
      181 atgaatttcc agcaaggtac tatcgtgaaa aatcacatac ggtggcacgc cttgctctct
      241 tgccagttcc agccgtttgg ctttaagtgc ttgccagagt gggtcatcat tggcaccctc
      301 aaatgcttct ttagcacggg cgctacgttc ggctttgctc atgcgttttt cgggttcggc
      361 atcacggcgt aaccagactt cttgctctcc ttttaacact gggcgtgcgg cttcggtgag
      421 atgcaaacct ccatagcctg acagatcagt ttctaataat ccggcggcaa tcagttggcg
      481 atagacgccg ctccactggg tttgattgag ggttttgcct atgccgaaag tgctgacttt
      541 atcgtgaccg aagcgctcga cttgcggggt gactttgccc atgagcacat caattaaatg
      601 gccgacaccg aatctttggc cggtgcggta tacgcatgag agtgccattt gcgcgacctg
      661 ggtggcttgc caggtttcta ccgggtgcag gcagttatcg cactggccgc aggcaccggg
      721 atggctctcg ccaaaatagc gcagaatgct ttggtgacgg caggcggtgg attcgcagta
      781 acctagcaag gcatccagct tcaggcgttc cagccgtttg cgttcttcgg aggcttcgcc
      841 gctatcaagc atttgccgca tggagacgac atcgcccatg ccgtagacca tccaggcatt
      901 ggcttgcagg ccatcgcgcc cggcgcggcc ggtttcctgg taatagcctt ccatactttt
      961 tggcaggtct aaatgcgcga caaagcgcac gttgggttta tcgatgccca tgccaaatgc
     1021 gatggtcgcc accatgatga tgccttcttc acgcaggaag cggcgctggt gatgttcgcg
     1081 taactgcgca ggcaaaccgg cgtggtatgg caaggcagac cagccttttt cgaccagcca
     1141 ggcggcggtt tcttcgactt ttcgccgcga cagacaataa ataatgccgg catcgtctgg
     1201 atgttcacgc tccagaaatg cctgcaattg ctggcgggca ttgtttttaa taccgacgtg
     1261 gtagcggata ttcggacggt caaagctaga gacaaactgg cgggcgtatt cgaggttgag
     1321 ttgactgatg atttcggcac gcgtgggtgc atctgccgtc gcagtgagcg caatgcgcgg
     1381 cacctcgggg aaacggttat gcaacactgt tagctggcga tattccggcc ggaaatcatg
     1441 cccccattgc gacacgcaat gcgcctcatc aatcgcaaat aaagcaaggc caacatgctg
     1501 ttgtacttca tcgagcaagc ttaaaaagct gccgaccatc aaacgctcgg gtgccacgta
     1561 caatattttg atttcaccac tcaccacctg gcgggtgata cgggcgttat cctctgcgct
     1621 taaactggaa tttaaaaagg cagcgttgac gcctaattgc tgtaaggctt cgacctggtc
     1681 ttgcatcagg gcaatcaaag gcgaaaccac aattgccaag ccatcgcgag ccaaggcggg
     1741 aatctggtaa cacagcgatt ttccgccgcc ggtgggcatg agcaccagcg catcgtcacc
     1801 tgcaatcaca tgctcgacga tggcttgttg ctggtggcgg aactggttgt aaccgaagac
     1861 ttcttggagg atttgttgtg gtgatgggct cgccatttaa atagcaagcc tagattttgt
     1921 tatatggatt gatcagttgg tctacgagtt cgtgtaaggc tttacgcatc tgttcttcac
     1981 tgacttccat cagcaagcca tcttccagcg catcttgtgc aatctgtttc agttcatcaa
     2041 aattttcggc catgactttg accttttcgg tgcaggaaac caccgaaccg tcatcgcgta
     2101 cccattttgg ccattcatta ctcatttaat tatttactca cctggttaat tactgagtgt
     2161 gtgcagttgt tgcagtagtt ttgccatcgc ttgcccacgg tgactcaact ggcttttgat
     2221 ctcaagcggt aattctgcca cggttttgcc tgtctgcgta tccagaaaca gtggatcata
     2281 gccaaagcct tcggtgccgc ggaactcacg caggatttca ccgcgccaca tgccatcggc
     2341 aataatgggc tgcgggtctt gcggattacg caccaggacc accacactat agaaataggc
     2401 acgtcgatcg gcgatgcgtt ccatggcagt caacaattta tcgttgttgc gctcgtcttg
     2461 cgtgagcagg cttttggtcg gttgtgcgcc ggcataacgt gcagagatca cgccgggggc
     2521 accttgcaag gcatcgacac acaagccgga atcatctgcc agtgcgggca aaccggtatg
     2581 ctggctggca tggcgcgcct tggccagcgc gttttcaata aaggtgcaat gcggctcggg
     2641 gcactctggt acatgcaact cggattgcgg caccacctcg atatttaagg gggccagcaa
     2701 atgcgcaatt tcacggattt tgcctttgtt accggaggca atgaccagtt tattgaaggg
     2761 caaggccatg cttaagcggc cagtgcggtt ttttgaatca cggaaatttc gctgatgcct
     2821 ttcgcagcca ggtccagaat ctggttcatg gtttcacgtg caaatggctc accttcggca
     2881 gtgccctgaa tttcgacaaa accacctttg ccggtcatga ccacgttcat gtcggtgtcg
     2941 cagtctgagt cttcaatgta gtccaaatcc aataccggtg taccttgata aacgccgacg
     3001 gaaatggcag cgacggaatc tttgatcggg ctttctgaca acaaaccttt ttgcagcaaa
     3061 gtgctcacgg catcgaccat ggcgacataa gcgccggtaa tgctggcggt gcgtgtgccg
     3121 ccatcggcct ggatcacgtc acagtcaaaa tggatggtac gctcaccgag tttttccata
     3181 tcaatcaccg cgcgcaggct acggccaatc aagcgctgga tttcctgtgt acggccggtt
     3241 tgtttgcccc tggccgcttc acggtccata cggctaccag ttgaacgtgg cagcatgccg
     3301 tactcggcgg tgacccagcc ctggttttta cccttcaaaa agccagggac tttttcttcc
     3361 acactggcgg tacagatgac atgggtatcg ccaaacttca ccagcactga gccttcggca
     3421 tgcttggtgt aatgacggat gatttggatg gcgcgcaact ggtcgttggc acgctgactg
     3481 ggtctgctca tggtaaacac tctctgctat ttaatatgtt gctgggttct gcccagcgct
     3541 tcttgtatct cggcaatcgc cttttcaatt tcgtcatcgg tcaggtccag accatatggg
     3601 ctgggcgtgg ccggactttc attcatcatg gtggtggtga catcgactgg taaatccagg
     3661 gtcgaaatgg ttaatggact caccacacgt tgtccccatt gcaaggcaat ggcgctcata
     3721 ttgtcaccca ccgtgatact ggctgcatcg gcatcatcca gcaggcggct gacgccatca
     3781 ttaatatccg ccgccagcat atgttgcgcc atattcatct caggcaccgt attccacacg
     3841 ccatccgagc acagcatgat gacatcgcct tcgcgtaacg ggataggcgg cgtcaactca
     3901 accttgggtg cgcgctcacc acccaggcaa ttataaattt tattgcggtg cggatgatga
     3961 tctacctcag ctgcggtgat ttttccttct cgcaatagcg actgcaccac agagtggtct
     4021 tcggtctggt gctcgaccag gccgttgcgg taatgataaa ggcgcgaatc accgacatgg
     4081 gcgcaaaaca gcttgttatg ctggatcagt gcgaccacga tggtggtgcg cggcgactcc
     4141 atcaggcgtt tttgcatccg cacattttca atcacctcat gtgcctggac gatttgcgtg
     4201 cgtaaaaatt ccgctggctg gacaattaat ggtttggatt cccgctgaaa tgcttccacc
     4261 agggttttga ccgcgacctc ggctgccaca tcgccatgct ggtggccacc catgccatca
     4321 gccaatacca tgaggatggc atgcctatgg taagaatagg ccagcctgtc ctggttgtaa
     4381 ggcctaggcc cctgccgact cccctgtgca atggtgaatt tcatagtggc ttgttcaata
     4441 ccttgatgat tttatccacc agcgatccac gcggattctc tgccacatgg atgccgatca
     4501 tttttttctg taatgagaac acgctttgcg gccgagacaa ataatccagt tgcatgcatt
     4561 catcaataat ggccagcaat ttttcagagt atatctgttt accgagctcg acagcaggca
     4621 ccaatgtatc ttctttcagg cgctggttgg ctgagactgg ggatgaccgc tgcaaacagg
     4681 catacatgct ggcgcctatg ctgtaaatat cactccatgg gcccaggtgc ttgcggtcta
     4741 tatactgttc aggcggtgca aaacctggcg tgtaggtcgg tgccagcgtc acctcagaca
     4801 aggcctgacg cgccgatccg aagtccagca gcatgggcga accatcgagg cggatataga
     4861 tattggccgg tttgatatcg agatgcagca gtttgcgggt atgcacctcg cgcaagccat
     4921 tgagcaattc aataaacatg cggataagga agttttcggg cacgggctgc gacaaggcca
     4981 tgatgtagtc ttgcagtgac ttgccgcgct cgtatttcat gaccatgtag acggtattgt
     5041 tggcacggaa gaagtttttc acacgcacga tatttttgtg ctcgatattg gccagcgcca
     5101 ggccttcttc aaaaaagcac ttcatgccgt gtttgtaggc tgcctcgttt ttaccattgg
     5161 agcggatatg cgtatgctcg tcgcgctcgg ccagcgtcac tggcatatat tccttgatgg
     5221 caacggtatt tttttgcgga tcttgtgcca gatagacgaa gctgaacccg cccagactca
     5281 acacccgtgt aatggtgtag tcatgcagcg ctgtgcctac cggcaaggca aaatcaaatt
     5341 tatttgagag cttcactagc gctttaacgt cacaatcatg gcttttatta agggaaaccc
     5401 tgcattcggt catttgaatt gcgggtttct tttatcgttg tagtattttc gcatatcgat
     5461 aacgcatttg atacaatcgt cccgtttgcc agcaggcccc atcaatccac caagaactac
     5521 gaagataccc acgacaatga ccgctaaaaa aaccgctcag gactacccta ttttaagcat
     5581 gaccggcttt gccgcaacgg agtcacgctt ccctggtggc ctgctgctga ttgagttgcg
     5641 cgcggtcaat caccgttacc tcgaactgaa tgtcaaaatt gacgacagct tgcgccagta
     5701 tgaagcgcac atccgcgagc aattacaaag ccgcctcggt cgcggcaagg tggaatgccg
     5761 gatgagcctc aaaagtgatc atgccgcgac gcatctggca ttgaacccgg ccatggtggc
     5821 acaaatttca caaacgctca gcgaattaca gcagcatttc ccacaggcac aaccggtgaa
     5881 cctagtcgag gtgtccaagc taccaggcgt gctgcagagc gaagccgtgg atgccgaagc
     5941 cctggcacaa gggctgctca atggattgtc acacgcgatt gacgaattga ttgccgcccg
     6001 ccagcgcgaa ggggaaaaac tcaagcaggt cttgctgaac cgtttgcagg gagtacgcga
     6061 gcagattagc atcgtcaaac ctttgttgcc ggcgctggta agccagtatc aggaaaaact
     6121 gacggccaaa ctgcgtgaag ccatgcaggc cgatgatgac cgtgtgcgcc aggaaatcgt
     6181 actctatgcg caacgcattg atgtggatga agagctggcg cgcctgcaat cccacattgt
     6241 tgaaatggac cgcatcctga gtgcaggcgg tgctgtgggc aaaaaactgg acttcctgat
     6301 gcaggaaatg aaccgggaag cgaatacgct gggctcaaaa tcggtggcga ttgaaaccac
     6361 gcaggtttcc atgcagctca aagtgctgat tgagcagatg cgcgaacaaa tccagaatat
     6421 tgaataaatc caacactgca aatactgtca gggctgcgtc agtaatgaat tggcgtttta
     6481 ttgcaaacat agtggtatag tcaacttacg tccaccatag taggctaaag accatgcatc
     6541 ttctgcgcta tgcttttctc cccttttgcc taatgccact agaggcaatg gctgaaacca
     6601 ccatgctggt gattcctgat caaggctggt ctgtgacctt tgatgcgcca cccttgcggc
     6661 ccgtgcgcga ggcagactcg aatgatcact acatgtactc tgccaacgcc aataactttg
     6721 ccgttacgtt gtacgtagat acgccgccgt gcaatgggga tgactcgcat gaagccgcgt
     6781 taaattgcta ttgggccaaa gcgggcaagg atccactgat caaccaggcc agcgtgacca
     6841 aaaattgtaa tcagcagtac tgcaaaatca gttacgactt tgaaaatagc tttcagggcg
     6901 cgtccatcaa acagaaacat gtccattttg tgtttacaca ccgggataga tggtcgaatc
     6961 ttcatgcggc ggtttccaat cccagcgaaa atgatttgaa gctgcttgaa gcatttacac
     7021 agtcttttag ctatcaataa acgtttaggc gtttgccgtt ctaacgttgc accacaatcg
     7081 agatgaacaa atgcactctt atggtgcatt ccgtcctttc gcattcactc aaattttaat
     7141 aacctactga tttaaataag aattgttagc tggcacacgg cttgctctta tttaattgaa
     7201 ttttacaagt aaggcaaagg tccagcatca tgacgcaatc agccaaacag ttttttgatg
     7261 aaatgcaggg ctatgggggc aatgtacggg cgatttacca ggcatataag cattggttaa
     7321 gcgatgtacc tacgcaacaa ctggccgcaa agcgtgccga agctgaagtt ttgttccgcc
     7381 gtgtcggcat tacattcaat gtctatggcg aggaagatgg cgccgagcgc ctgattccgt
     7441 ttgatgtgat cccacgcatg atttcggcca gcgagtgggc ccacctgtcc aaaggggctg
     7501 tgcagcgtgt gcgcgcactg aatatgttcc tgcatgacat ttatcatgac caggaaatca
     7561 tcaaggccgg cattgtgccg cacagcattt tgagcaatgc gcaataccgg ccggaaatgg
     7621 tgggcgtgaa tgtgcccaac caggtctacg cccatgtggc gggtatcgac ctggtccgca
     7681 ccagcgaatc ccagttttat gtgctggaag acaatctgcg tacgccatct ggcgtctcct
     7741 acatgctgga agaccgcaaa atgatgatgc ggctgttccc agatttattt aagaaatatc
     7801 cgattgcgcc ggtagagcat tacccacatg tattgctcaa caatttgcgc tcggtcgccc
     7861 aaaccggtgt caccgacccg accgtggtcc tgctttcacc aggcgcttat aacagtgcct
     7921 actttgaaca tgccttcctc gcccagcaaa tgggtataga actggtggaa ggccaagatt
     7981 tgtttgtccg taataacgcc gtctacatgc gcaccacaca aggcccgcaa agggtggatg
     8041 tgatttaccg ccgcatcgac gacgacttcc ttgaccctct ggtgttcaag cccgattccc
     8101 tgttgggcgt gccaggtctg ctctccgttt accgcaatgg tggcgtcaca ttggctaacg
     8161 ctgtaggcac cggcgtggcc gatgataagg cgacgtatat ttacgtgccg gagatgatca
     8221 agttctacct gggtgaatct cccatcctgc agaacgtgcc tacgtatcaa ctcagcaagg
     8281 aagatgatct ggcctatgtg ctagatcatc tgcccgagct ggtcgtcaag gaggtacaag
     8341 gctccggcgg ctacggcatg ctggtcggcc cgactgccag caagcaggaa attgaagaat
     8401 tccggttgcg tattgtttcc aaaccatcga attacattgc gcagcccaca ttggcactgt
     8461 ctacctgccc gaccttggtc gatcagggca ttgcgccacg ccatgtcgac ctgcggccat
     8521 ttgtattgtc cggtaaaacc gtcagcgtgg taccaggcgg cctgtcacgc gtggcactta
     8581 aagaaggctc actcgtcgtt aactcatcac aaggcggtgg taccaaagat acctgggtgc
     8641 tggagcgtga tgatgtctgt gtcgatactg cgcagtcaca aatggctgcc caacaaatga
     8701 atcaagaggc cgtcatatgc taagccgcac cgcagaccat ttatactgga tggcacgtta
     8761 tattgagcgc actgaaaacg ttgcgcgcgt gctggatgtc acctataaca tgtccctatt
     8821 gccctcggac agcagcaatg aagaagagct gtgggacacc gtgctcgaaa ttgctggtat
     8881 ccgcgatatt tatgacgcga cttacaaaga gctgaatgcg gctaatgtga tcaaacacct
     8941 ggtcatggat agccacaacc catccagcat ttacagttca ctgcgtagcg cacgggaaaa
     9001 tgcacgcgcc gtacgcgttg ccatgaccac cgaaacctgg gaaaacatga acacgctctg
     9061 gctggaattt ggccagtaca tcaagcagga cctgacaaaa accggcctca gcgagttttg
     9121 tgagtgggtc aaaagccgtt cacatctgtt ccgcggggta tgctttggca ccatgctgcg
     9181 cgatgaggcg ttcagttttg tccgcctggg cacgtttatg gaacgtgcgg acaacacggc
     9241 acgcctgctg gatgtgaaat atgaattttt aatccccatg caggaagcca atgatggcgc
     9301 agtcgactac tacgaatgga gcgccgtgtt gcgctccgtc tcggccttcc aggcctatca
     9361 gaaggtttac cgcgatgcga ttgaaccgat gaaagtgtct gaactgctca ttttgcgtga
     9421 agacatgccg cgctcactgc atgcctgcta tgccgaaatc atgccgattt tgcgccaggt
     9481 ggccggtagc cgccagaccg aagctgagcg tttggccggg caaatgcatg caaaactgca
     9541 ttatggccgc atgctggata ttgtccgcga tggggtacac catttcctgg acgaatttat
     9601 cctggccaac cgcaagctgg gcgaagaaat acaacgtaat tttttaagtg cgacagcctg
     9661 atagaaacgc acgcatgata acgccataga ttgccatgaa ccccctcatg cgcagactgt
     9721 tgtaaaacag cgctgcgctt tttactttaa ccgataccct gcatcatgct gcttaatatt
     9781 gaacaccaga cccaatatgt tttcagtgaa gtcgtgcaat acaccataca acagttgcgc
     9841 ctgacgccgc gcaacgggta tgggcaagcg gtcaaacact ggcagatcaa gatgaatggc
     9901 caattaacgc catttgaaga tgcttttggc aatctctcgc ataccctggt catagaccag
     9961 ccgcatgact cattgctgat taccgtctcg ggtgcagtag aaaccgggct gcaaacagaa
    10021 caacccgaac aggctctgcc accagaaatt tacctgcggg atacaccgct gacacaggca
    10081 gatgccgcta tgctggaatt tgcccggccg tttgcgaatg ccccgctcac cgcgctgatg
    10141 caggccctgc gcgaacgtat gcagtacatc aaaggtgcaa ctcaggtaga cacgcccgcc
    10201 gctgaagctt tccggcttgg caaaggcgtc tgtcaggacc atgcgcacgc ctttatcggc
    10261 tgctgccgta gcctggggct gcctgcgcga tatgtcagcg gctatttgtt tactgtggat
    10321 ggcagcctca tgcaaacgca cgcctgggcc gacgtctggc aacaagggca atggctgggc
    10381 ctggatgtct ctaacggcac attggttaac gagacccatg tcaggctagc acacgggctg
    10441 gattaccgca gtgccagccc ggtgactggt tcccgcatgg gcggtggcat cgaaggcatg
    10501 gccagcgtgg tcagtgtgaa ccagcaagaa agccgggcca agccacaaga ccaggcgcgc
    10561 ttgctgcaac aggcgcaaag tgcgcagcag tagctggttg ctgcgtgcta tgatgcccta
    10621 tccgatttta ttttctaacc gctaacgctt atgacttact gtgtaggtat tttgctggaa
    10681 caaggcctgg tcctggcatc tgacacacgg accaatgccg gcgtggacca ggtggccatt
    10741 gcgcccaaga tgcatgtgtt tgaggtgccg ggggagcgca tcattgtgct gctcacggcc
    10801 ggcaacctgg ccattaccca gtcagtagtc aatctgtgtc gcgaacaact caagtccccc
    10861 gaaccgcatg cccatttgca taatgtgcaa tcactatttg aagcggccag tgtggtcggc
    10921 gccgccatgc gtagcgttca ccagcgcgac ggtaaagccc tgaaagaaca cagtattgat
    10981 tttagcgcca gcattcttgt agccgggcaa atcaagggcg agaagccacg cctgtttaat
    11041 gtttacgctg ccggtaactt tattgaggct acgccggaca ccccctattt gcagatcggc
    11101 gaaaccaaat acggcaaacc catcatagac cgtgtcgtca aacatcaaac caatgtcaca
    11161 gatgcggtaa aatgcgtgct gatttcattt gactccacta tccgcagcaa tatttctgtg
    11221 gctttaccga ttgacttgat gatataccgt actgacagtt taaacgccga ttgccggcag
    11281 cgcctgatgg aagatgaccc ttattacgcc agtatccgca agggctggag tgaaggtttg
    11341 aaagccgtgt tttcttcatt acccaacccg gcctggagca cctcatgagt catggtcatt
    11401 tgtttgtgat ttctgccgcc tccggcgcgg gcaaaaccag cctggtacgt gccatgctgg
    11461 cgcaagatcc catcctaaaa ctgtcagtct cacatacgac gcgtgcgccc cgtccgggtg
    11521 aggaagatgg cattcattac cacttcgttt ccgaaccaga gttcctgcaa atccttgata
    11581 gcggtggctt cctcgaaagt gcgcatgtgc atagtgcacg ttatggcacg gcgcaaagtg
    11641 aggtcgaaaa tgcgctagcg gctggccgcg atgtcatcct ggaaattgac tggcaaggcg
    11701 cacaacagat acgcacacta ttcccgcaag tgacctctat ttttatcctg ccgccttcta
    11761 tagaggccct gtcgcaccgc ctgaacagcc gtggccagga cagcgaggcc gtgattacgc
    11821 aacgtttggc agctgcccgc gaggagatgc gtcacgtgca tgagtttgat tatgttacaa
    11881 ttaatgataa ttttgacgtg gccttgcagg atctgatggc gattgttcgc gcccagcgct
    11941 tgcagttaaa ccgccaggtg gcctgccacg cgtcattgat tcaatcactt acctgaccca
    12001 gcgcctgacg gcactgaccc aggctccaac ttagaggttt ttatggcacg tattactgtt
    12061 gatgattgct tggacaacat ccctaaccgt tttcaattga cactggttgc ggcttaccgc
    12121 gccagacagc tggccaatgg ttccgatcca ttagtgagtg gttactcaca aaaagataaa
    12181 gccactgtgt tggccttgcg cgaaatcgca gccggcaaaa tcggtgttga gctactagag
    12241 aaaaagggcc aagcataagc aactatgccc cacacccaag gcgattctgc ccaccgcaag
    12301 tctaggcctc ccttggagga ggtcgatttg ctaccggctg accgtgagga ttctcccctt
    12361 acccagctga tccgcaagta tctcaatgaa gaagaagtgc aactggtctg gcaagcgtac
    12421 cgctatgccg accaggcgca tgccggccaa acccgcaaaa ccggcgaacc ctacatcacc
    12481 cacccgattt ctgtggcctg catcctggcc aggttacatt tggacctgcc cacgctatta
    12541 gctgccttac tgcacgacgt cgtcgaagat accgaagttg acagtgcggc cattaccgaa
    12601 cgctttggca aacaggtcgc agacctggtg gatgggctga ccaagctcga taaggtagag
    12661 ctgcaaacgg ccacccaggc ccaggccgaa aatttccgta aaatgctgct ggccatgagc
    12721 caggacgtgc gcgtaatact ggtcaagctg gcagaccgtt tgcacaatat gcagacactg
    12781 gatgccatgc gcgcggaaaa gcaaaaacgc attgcacgcg aaacgctgga tatttatgcg
    12841 ccgattgcca atcgcctggg cttgaacccg atttaccatg agctggaaga tctcagcttc
    12901 aaatacctgt accccaaccg ataccgcgtc attgataagg ccatccgcac tgcccgcggc
    12961 caccgcaaag aggttgtcag caagattcaa gacgccatcc agaaccagct gacgcaatac
    13021 cagattgaat atgacgtaca gggccgcgaa aagcatttgt acagcattta caaaaagatg
    13081 tcagaaaaag cgatgaagtt atcgcaaatt ggcgacattt acggttttag ggtcatcgtc
    13141 aaggatacgg cggcctgcta cctggcctta ggcgccctgc acgctttata caaaccgatt
    13201 cctggccgtt ttaaagatta tattgccatc cccaaggcca atggctacca gtcactgcat
    13261 accaccttgt tcggcccctt tggtacgccg attgaagttc agatccgcag tcaggaaatg
    13321 cacaatattg ccgatgccgg tgtggctgcg cactggttgt acaaaacgac cgatgcccat
    13381 ttgaccaagc tgcagcagca aactcaccag tggttacagc gcctggtgga aatccagacc
    13441 gaaagtgatg acagccacga gttcctggag aacttcaagg tggacctgtt tccggatgag
    13501 gtgtacgtgt tcacgcccaa aggcaacatc atggctttgc cacgcggctc aacggcggtc
    13561 gactttgctt atgccgtgca ctccgatgtc ggtaatagct gtgtggcggt taaaatcaat
    13621 aacgaactgg cgcccctgcg tactgaaatg cgcaacggtg accatgtgga aatcattact
    13681 gcgccgctgg ccaagcccaa cccggcctgg ctcaattacg tggttactgg caaggcacgt
    13741 tcgcatattc gccattacct gaaaacggtg aaggtggaag aatctgcaca attgggcgag
    13801 cgcatgctga atgtggcatt acgcgccatg catattgaac ccgcagcgct gtctgacaag
    13861 cagtggcaaa aagtcatgaa tgactatggc gtcaaaacgc gccaagctat tttgaccgat
    13921 atcggccttg gcaagcgcaa cagcctcatg gtggcccacc agttggtcgc gcatccagca
    13981 gaggctgaag aaaagtctgc caaagtgctg gataccatca ccatccgtgg ttcagaaggc
    14041 atggccgtac aatttgccca atgttgcagg ccgattcctg gtgatcctat cctgggcttt
    14101 attaacaagg ataaaggcct ggtcattcat acccatgact gtccggccat ccgcaaattc
    14161 aagctggatc cggaaaaatg gctggatgtg gaatgggacg ccgatccgaa gaaactgttc
    14221 agcgtcaact tgcgcgtcac cgtggtcgac cagcgcggca tgctggccaa gattgcggcc
    14281 accatctcca atgcggatgc caacattgat aacgtgagtg tcgaaaccag tgatggcagc
    14341 cagtattcca atgtcaactt cacggtgcag gtgcacaacc gcgtacacct ggcgaccctg
    14401 atccgcaacc tgcgcaagat ccagcaaatc gtgcggatca accgggttaa aggcaaccag
    14461 ctggaacaac gcatccagta acccgcccct gcccgcatgc gccccttgag cgacatcaaa
    14521 ggttttacgc ctgccttgtg tagccagctg gataaactgg gcatccgcag tgtcgaaagc
    14581 ctgttgttgc atttacccct gcgctacatc gatgaaaccc gtatcacggc catccgcgat
    14641 ttacggctcg gcgagcaaac ccaaattgaa ggggtggttg tccatcagga ggtgagttac
    14701 aagccgcgca aaacgctcaa tgtccgggta caagacgata ctggcgtcct caacctgcgc
    14761 ttcctgcact tttaccccag ccaggtttcc gccatgcaga caggccagcg cttgcgcgtc
    14821 tttggcgaag tccgtcccgg attttttggc tatgaaatgg tgcaccccac ccagcgcaaa
    14881 gtgaaggagg acacccccgt caacagcgca ctgacaccag tctaccccac caccgccggc
    14941 ttgagtcaat cggccattcg caaggcggtg aaactggtct tgcatggtga gcacctgcac
    15001 gagaccctgc cggactgggt gtatcaaggc atgcgcctgc cagattttgc cagcagccta
    15061 cggctgttgc accagcccag tccagagaca gcactcaaca ccctggaaga ccgcacacac
    15121 ccggcctggc agcggctggc ttacgatgaa ctactcgcgc aacagctgtc catgcgccag
    15181 cattatgcac gccgcaaaca atcacaggcg cccgccattt ccggctcaca acacctggtc
    15241 gcccgcttgc ttgccagctt gccgtttgca ctgacgtcag cgcaacaaaa agtggtgcat
    15301 gaaatccagc acgacctggc gcaaccccat cccatgcaac gcttgctgca aggagatgtg
    15361 ggtagcggaa aaaccatcgt cgcctgcatc gcggccttac agctgattga gcatggctgg
    15421 caagtggcca tcatggcccc tacagaaatc ctggcggaac agcattacaa aaaattgtgc
    15481 ggctggttac agccgttggg catcacggtc gcctggctca ccggcagcca acctaaaaag
    15541 gaacgtgagc aatcactctc actcattgca aacggtgacg ccatgttggc cgtaggtacc
    15601 catgccttgt tccaagatga tgtcgagttc aaacaactgg gtttagccat tgtcgatgag
    15661 caacaccgct ttggtgtggg gcaacgcctg gcgctgcgca aaaaagggac cggaaaaaac
    15721 gacacggtat ctcgttggga accgcaccag ctgatgatgt ctgcaacgcc tatcccacgc
    15781 actttatcca tgagttatta cgccgacctg gatgtatcgg tcattgacga attgccaccc
    15841 ggccgaacgc ctgtgatcac caaactggta gccgatgaca ggcgcgaaga aattttgcaa
    15901 cgcgtgcatg atgcctgcgc ccagggccac caggcctact gggtatgccc gctgattgaa
    15961 gaatcagaag ccctgcaact acaaactgcc aatgatacct ttgcctatat gcagcaggct
    16021 tttcccgagt tacgtgtggg cttggtgcat ggccgcatga agacggccga aaaacagacc
    16081 gtcatgcagg cttttgccgc caacgacatt cacttgctgg tcgccaccac ggtgattgaa
    16141 gtcggcgtcg atgtccccaa tgcgagttta atggtaattg aacatgccga acgcatgggc
    16201 ctgagccagc tacaccagtt acgtggccgc gtcgggcggg gatcagccaa aagtacctgt
    16261 attctgctgt ataacaagca gcaatgttcg caaattggcc gccagcgtct caaggttatt
    16321 tttgaaagca gcgacggctt cgagatcgca caggcagatt tacatatccg gggtccgggt
    16381 gaattactgg gtacgcggca gagtggtgtg cccatgctca agattgctga cctggaacgg
    16441 gatgctgacc tgttggacat cgccaaaacc atggctgacc agttattgca acgtgcacct
    16501 gcagccgtat ccgcgcattt gcaacgctgg ttaagccagg cagaggattg ggtcaaggtc
    16561 tagcagatca ctgcaaacta ggcttgcccc ggcaatgctg acgttttttc tctggctgag
    16621 gatttttttg tccgggacat ggctgccagc agattcgctt caaagtcctg caaagggagt
    16681 gggcggctaa acaggtagcc ctgaaaacaa tggcatccca tctcttgcaa caaggtcatt
    16741 tgctgttgca tctccacccc ttcggcaatc acactatggc ccaggttttt gcccatattg
    16801 ataatggttt gcacaataaa ggcatcgtaa ctgttgagca caatatcctt gataaaactg
    16861 cggtctattt ttagctcgtc gagcggcagg cgcttcaata ccagcaacga agactgcccg
    16921 atcccaaaat catccagtga gaagctgatg cccagcgccc tgagcgcgga aattttacca
    16981 atcacttcat ccaggttttt tacaatcagg ctttcggtca actccagccg caaacgcctg
    17041 gcatcgcagc cgctagcctg taccgtctca gtcacggtgg caacaaattc aggctggcta
    17101 aactggatgg cactcacatt aaccgataat tgcagtgatg ccgtcagcgc agatttcgac
    17161 cactgctgga gttgctggca ggcctgcgcc agcacccatt gcccaatcgc gacaatttcg
    17221 ccggtgtcct ccgcaatcgg gataaactga tcaggcggga tcatgcccag cgtcagatgt
    17281 tgccagcgca ataacacttc tgcccctatc ggctgttgct cggcatcgac ttgtatctgg
    17341 tagtacaaca ggaactggtt ggcctgtagc gcaaatgcca gctcattttc cagcgtcgcg
    17401 cgcatttcta tcgccggatg cgtcgcttcg tcatacatct gtatgctgtt caggttatcg
    17461 gattttttgg cctgatacat ggcaatgtcc gcacgcatta acacttccgc ctggctacat
    17521 tgatcgccga gaaacaagca aatcccgatg ctgggttcag tacgatacct ggtattctgc
    17581 aactggaatg gttgctcaaa caccgccatc attttttcag ccgcaacctg cgcggcagct
    17641 gcggcttctg ccttgtctgt cgataactca tccagtaccg caataaactc atctccaccc
    17701 atacgggcaa tggtgtcctt tttgcgcatg cattcctgta aacgggccgc cacctgctgc
    17761 aataattcat cacccgcctg atgaccttta ctgtcattga gccctttaaa attgttcagg
    17821 tccagagaca agactgcgcc gtaggtttga ttgcgtacac accgcgcaaa agcttggctc
    17881 aggcggtctg ccagcaatcg ccggttcggc aaaccggtta aaaaatcata ataagccagc
    17941 tggtggactt tttgattatc gagattggag gcaatgcgta aggagatatt ctcgcgcaaa
    18001 ctgagcgcca ggccacgccc tgccacggtg acataaacga catagacgat aaacaagacg
    18061 gccagggcca gcgatacgga actgccatgg atgaggaagc gtggaatcat gaacaacaga
    18121 atgccaccag taaacaggtt gctgctgaag gtgtctaccg aataaataac aatcccggca
    18181 ccagacaccc ccaccagcgc aaaaatcagg aaggcctgat gtgcagtatc gctctctgga
    18241 aacagtagaa tgccagccaa cccccaggca atgccgcaaa aaccggtact ggcacgaaac
    18301 cactgcagcc actggcggct atgtcgcgca tgctgctggt ctttttcata ctgatacgtc
    18361 agaaggccgc gcgtaccata ggcggcaatg aaaacagcag accatatggt caggatgaca
    18421 ggattaatca gcggccgctg cacaagcaat aaggcaatca tgaccacgat cgcggccagt
    18481 agtgatctgc gattgtttgc ataaagcatg cccacaattt cagactgcaa tgcggatgat
    18541 tccgccgttt tgttcactgc ccgcaccccc tgcaattatt gtttactgct caacgggtca
    18601 atctggttaa cgctatgcag ttatcatgcg ctcaaacatg aaaaaaaaca ctattccaca
    18661 gtgcgttttt agtccaaaaa tggcaaaaaa actctaagaa atgtcagcgg tgcaaccagt
    18721 gttgcacaag cttgacgtaa cgcggcatca ccacatacac catcagcgcc accaccacgg
    18781 ctgtcacaaa aaaagtatgc acaatgcggt tggggggcaa accagcctca tccaggaggg
    18841 ggacaaacag ccatggcacc acgctgacca gcggaaaaat ggctgaccag gtcactaaaa
    18901 actgtttcca gcgtacaggt ggtttggcgg gggccacatt cggctgcata aacaaaaagt
    18961 ccaggccact actaacccgg tactcttcct gcccggccag taagtgtgcc gctttggcga
    19021 tcagggcctg cctttgcggc gaatcgagcc actgtgtgag atgccgctcc gtttcatagc
    19081 ggatcatcac cgaataagta tcggtatatc cggcaatcgg ccggataata tgccagtcga
    19141 gcaggccttc cgaggatcgg cacaccggcc cgatttcctg taaccaggtt tcatattcgg
    19201 cctgtaatgc cgggctcaca tggtgggtaa tcaccacgct ggcccctttg gtttccggga
    19261 ttaaagcggc attgtttgtc atgcaaaggc tcctcgcctg aaattgatta tgctgttatg
    19321 aatgaaacag tggttttaac tgggcaacgg ccaagtcaat aaacccacgt aatttgggcg
    19381 gcataaaacg ctgtttcgga tataccaggt ggaccgcatc tgcctgcccg cgccactccg
    19441 gtaacaccct ttgcaagcct cccgcctgta ccgtaggctg gcagataaag gtgggcaaca
    19501 aggccacccc ctgcccgctt tcggccatgg cgcgcaccac ccctatgctg ttgaccagcg
    19561 tacgcgccgc caggggcaca cggatcgccg tggtgccacg ttgtaattcc caatggtggc
    19621 gacccatgga cgtgaactgc acacattgat gctgagccag atccgttggc gtcgcaatcg
    19681 agcctgcctc ctgcagataa gcggggctgg caaacaaggc ccattgtatg gtgcccagtg
    19741 acttggcaat caggctggag tcgcgcaact cccccgtgcg tatcgccaca tccacgcctt
    19801 ctgccacgag gtcgacataa ctctgtgtca gcatcagctc cagcgacaag gcaggatacg
    19861 tctgctgcat ctgcgcaatc aggctaccaa gcaacacatc gcccagatcg gcaggggccg
    19921 tgacttttaa tacgccctga ggttgctgac gcgcttcatc cagtgctgcc tcagcctcca
    19981 gcaaataacc gagcccggta gatgcatgtt caaaatacag tgtccccgcc tccgtcaggt
    20041 tcaacttgcg tgtcgtacgt tgtaacaggg tgacgcccag ccgtttttcc agacgtgcca
    20101 cccgcgtgct aatggtcgac gtcggcaaac ccagctgtct ggctgccgcg ctaaaactcc
    20161 cggcctgcac caccctcacg aataccgcag cctcattaaa gtccatgctc tattattcat
    20221 ttaattgaaa agtcatttca catttaacca tctagtgtat taaaagtcat ctgtttatag
    20281 tgtgaaccat gcaatcacgc acaacgaatc aacaaagctt ttgaaaggaa acaccatgaa
    20341 aaaactggct tttatcaaac gcagcaacgg caaccactgg gtcggtgacg gctttccggt
    20401 acagagcatt ttttcctacc gcgatatcgc caatgatatt tcacctttcc tgttgatgga
    20461 ttatgccggt ccggccactt ttgaaccaac cacagaacgc cgtggcgtcg gtcagcaccc
    20521 gcaccgtggg tttgaaaccg tgaccatcgt ctatgacggc caggtctccc accatgactc
    20581 caccaatgcc ggtggcacca tcggtcccgg tgaagtgcag tggatgaccg caggcaaagg
    20641 cattatccat gaggaatatc atggcgatga ttacgcccgt accggtggcc cgttcgagat
    20701 gatccagttg tgggtcaact tgccggccaa ggacaaaatg actgcacctg gttatcaagg
    20761 catcaccaaa gccgagattc cagaagtggc tttgccggat gatgcaggta aggtgcgggt
    20821 gatcgctggt gaatacgcag gccagaaagg ccctgccagc accttcagcc cgatgaatgt
    20881 gtgggatgtg cgcctgaatg ccggcacacg caccaccttc accctgccag aaggccacac
    20941 cactgccgtg tttgtactgc atggcgcggt gaaaaccggg gaagtgcata ccatccgtcc
    21001 tgccgagctg gccgtgatgc agcgcgaagg taacgagttg gtattacagg cgcagcaaga
    21061 caccgtgctg ctgttactca acggtgaacc gctgaatgaa ccggtcgtcg gccatggccc
    21121 gtttgtcatg aacacctggg aagaaattga ccaggcaatt aatgactaca accagggccg
    21181 gtttgccact gcctagattt aacaatttta ttaaaacagg aaacacggct gtcagcactt
    21241 tgcagtaacg ctgatggctg tgtcccacta acttaaatat cagggtactg ctaaaaattt
    21301 atttggctaa gccagacaag acgcgattaa aaaattttga cgccgcatat atgaaatatg
    21361 tcaggagaaa ttttttatga acaacgccgt atggcaacgc aaaatggatt tttagcagtg
    21421 cccgttaaga ggatattcat catgaaaaac aaaccttatg tacgtctgga ccgtgacaac
    21481 gctgtcgttt tgctggtaga ccaccaggca ggtctgctct cactggtacg ggatatcgag
    21541 ccagaccgct tcaaaaataa tgtactggcg ctggccgatg ctgccaaatt ttttaacctg
    21601 cccaccattt taaccaccag ttttgaatcc ggccctaatg gccccttgat gccagagctc
    21661 aaagccttat tccccgacgc gccctacatt gcccgccctg gccagattaa cgcctgggat
    21721 aacgaagatt ttgttaaagc gatcaaggcc accggcaaaa aacagctgat tatcgccggt
    21781 gtcgtgactg aagtctgtgt tgccttcccg gccctggcag ccatcgaaga aggattcgac
    21841 gtgtttgtcg tcaccgatgc ttccggcacc tttaacgaaa tcacccgtga tgctgcctgg
    21901 gaccgcatgt ccgccgccgg tgcacagctc atgagctggt ttggtgtggc ctgcgaacta
    21961 caccgcgatt ggcgccggga tattgaaggc ctgggcaccc tctgcgctaa ccatattcca
    22021 gattaccgca atttgatgac agcttttaat gcgttgcaag gcaaataacc aaacacttgc
    22081 aaaggtgaga atttttttaa aacatatttg tctaaatata tccattacaa gtttttgttt
    22141 aagtaaagcg tttcctacaa cacaatagga tttctgctta ttcagtattc aggaccatca
    22201 gcgtaatctg ca
//
LOCUS       DATMSK010000272         5672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_162686,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000272 DATMSK010000000
VERSION     DATMSK010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162686"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..2591)
                     /locus_tag="VNZ84_09125"
     CDS             complement(66..2591)
                     /locus_tag="VNZ84_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWU67161.1"
                     /translation="MKNPIARLNVGTRLFLGYALLILFTTFIFVIAWRGFNHIHQTWQ
                     HFENVQLVKRTQIENASKGLGDAIHHFKNYVLRGGREATKFEHSMSDIEQAMAAYQAT
                     GEISAQEQEILGNIALALTTYRDAIKRCEALKNDGMSVTEIDSTVKGADKPLNHSLRL
                     LMDMNIAGVTDASSQFASTIASANKQIETALLVAVVLAIAIAWKMMRSITTPLNQALT
                     LSKHIANGEFIAQEAPTGNDETAKLLREMHRMQSTIQQIVESLNEMSSQHRAGMIDYR
                     IDSQPFHGAYQQLTTGINQMVEEHILAQTKSLQVVTAFGQGDFHQPLETFPGQRSYIN
                     QSIEQVRQNFKLLIEDVHTLSQAAEQGQLNIRVDVSRHHGEFKQIVAGFNQTLDAVVD
                     PLQTTARYLDEMAAGNIPTQIEEHFVGDFNLMQTSLKHCTGAIHQLITDVHHLASAAQ
                     QGQLNTRVDVDAHAGDFRKIIHGINQTLDAVVTPLHVAADCVNRIAAGDIPAPIHNEY
                     HGDFKVLINNLNTCILAVNRLVEDTRQIADEAQQGQLYSLVDVTAHTGDFRLALQGSQ
                     EILQRMVAPIHTVKQAAETIHVAAAEIEKGNNDLSRRTETQANALQETSANLETLTNN
                     IRQTAEHAHQASLQASQANQMAVQGGEAFNALTTTMQGINETSAQIAQITSVIDSIAF
                     QTNILALNAAVEAARAGEEGRGFAVVAGEVRNLAKRSAASAKEIKELIAASVSQTNAS
                     AKQVSQAGHTMHEIVNAVQQVSHSIQVISEASSQQSSGLEQIYESMTSIDETTQQNAA
                     LVEEAAAAAESMLEQVHTLVESVNQFKMDADDTNDHGHLRLIA"
     gene            2849..3331
                     /gene="greB"
                     /locus_tag="VNZ84_09130"
     CDS             2849..3331
                     /gene="greB"
                     /locus_tag="VNZ84_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HWU67162.1"
                     /translation="MAEHKNYITPEGYAALEKEFQYLIKVDRPEVVRVVSWAAGNGDR
                     SENGDYIYGKKRLRQIDSRIRFLTKRMDAAEIVYSNTQTNTEKVYFGAWVTLFNLNDD
                     SEMTIRIVGQDELDPAQGYISWVSPMAKALLGKNLGDTIRVITPAAETEYEIIDIRYQ
                     "
     gene            3395..3769
                     /locus_tag="VNZ84_09135"
     CDS             3395..3769
                     /locus_tag="VNZ84_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67163.1"
                     /translation="MRKRLCLFLICWFPCFVMAANVMSMQMALAQHALPNMVQVQSAE
                     MPCHHMADASHETQHTTEQHAPGNHSCTVCGFCMVSTGVAHLDLFPSISIIVQSAAAP
                     LYVADPVHSQTYPPAIKPPIFG"
     gene            3844..4530
                     /locus_tag="VNZ84_09140"
     CDS             3844..4530
                     /locus_tag="VNZ84_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67164.1"
                     /translation="MFKQHHVAPFNISLVSSSLGALLLGQIALSAHAAALMGFEDSAM
                     LMSEIGRYNQEWMLNYSPTFGHAIGVEQMRMSGKGERATTISGINYTGIIKRWNLPAA
                     QANVWFNGSVGEARGQYDGFAYTPSLQFDVESTRLYFLAKARMIRAPHMNFDTAAVQA
                     GFSFYETGFNDTQPWFVIEAKTMRNNDPGLQVTPALRLINKNYFLEFGVTNPWQGEGF
                     APRLNAMFVF"
     gene            4559..4900
                     /locus_tag="VNZ84_09145"
     CDS             4559..4900
                     /locus_tag="VNZ84_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HWU67165.1"
                     /translation="MKKLWIALMLSAMTPAAFAVTTIKAEVNGMVCAFCAKGIEKKLN
                     ALPQKQAAFVDLKSRVVALQLKEGQDVSNEAFSKVIEDAGYSVSKLERVNQTVAVIQA
                     DVANTEKAGGK"
     gene            4900..5292
                     /locus_tag="VNZ84_09150"
     CDS             4900..5292
                     /locus_tag="VNZ84_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67166.1"
                     /translation="MAAEINHLRANKRLNLLSLFTSGSTLICCALPATLVAIGSVATL
                     TSLISHFPQLIWISEHKPLVFGLAGAMLLLAGWLQWRARLLPCPADPQLATLCTKTRR
                     QSLWIYWVSVMIFLIGAFFAFIAPLLIE"
     gene            complement(5365..>5672)
                     /locus_tag="VNZ84_09155"
     CDS             complement(5365..>5672)
                     /locus_tag="VNZ84_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWU67167.1"
                     /translation="FRPILDKFAAGLQNNAAARVTIVGHTDSSGSDAINDPLSVNRAA
                     SARSYLVERGIAISRITIDGRGSHEPIAANDTEANRAKNRRVEIFVAEPEQPPATPK"
BASE COUNT         1320 a         1343 c         1494 g         1515 t
ORIGIN      
        1 gtcagtcatc acggtatgaa taaaaaatgc tccgcgaagg agcatttttt attggagcga
       61 acccgtcagg caatcaggcg taaatggccg tggtcgttag tatcatcggc atccatttta
      121 aattgattca ccgactcaac cagcgtgtgc acctgctcca gcatgctttc agcggctgct
      181 gcggcttctt caaccagggc tgcattttgc tgcgtggttt catcaatgga tgtcatggat
      241 tcataaattt gttccaggcc gctactttgt tgactggagg cctcggaaat cacttgtatg
      301 ctatgggaca cctgctgtac ggcattgacg atttcatgca tggtatgacc cgcctggctc
      361 acttgtttgg cgctggcatt ggtctggctc actgaggccg cgatcagctc cttgatttcc
      421 ttggctgaag cagcagagcg ttttgccagg ttacggacct caccagccac cacggcaaag
      481 ccgcggcctt cctcgccagc gcgtgcggct tctaccgcgg cattcagcgc caggatgttg
      541 gtttggaagg caatgctgtc aatcacgctg gtgatttggg caatttgtgc cgaggtttca
      601 ttaatgcctt gcatggtggt ggttaaggca ttgaatgctt cgccgccttg tactgccatc
      661 tggttggcct ggctcgcttg caggctagcc tggtgtgcat gttcggcggt ttgccggata
      721 ttgttggtta gggtttccag gttggcggag gtttcctgca aggcattggc ctgggtctct
      781 gtgcggcgag acaggtcgtt gttgcctttt tcaatctcgg cagcggcaac gtggatggtt
      841 tcggcggctt gcttgacggt gtgtatcggc gccaccatgc gttgcagaat ctcttgtgag
      901 ccttgcaggg ccagcctgaa atcgccagtg tgggcagtga catcgaccag gctatacaac
      961 tgaccctgtt gtgcttcgtc ggcaatctgg cgcgtatctt ccaccaggcg gttcactgcc
     1021 aggatgcagg tattcaggtt gttaatcagc accttgaagt caccgtggta ctcgttatgg
     1081 ataggcgctg gaatatcacc cgcggcaatc cggttcacgc aatcagctgc cacatgtaaa
     1141 ggtgtgacga cggcatccag tgtctggttg atgccatgga taattttgcg gaagtccccg
     1201 gcatgggcat caacatccac cctcgtattc aattgccctt gttgtgcggc tgaagccagg
     1261 tgatgcacat cggtgatcag ttgatgaatt gctcccgtgc aatgtttgag gctggtttgc
     1321 atcaggttaa agtcaccgac aaagtgttcc tcaatctggg tcgggatatt gcccgcggcc
     1381 atttcgtcca ggtagcgcgc cgtcgtttgc agcgggtcta ccacagcatc cagggtctgg
     1441 ttaaagccag caacaatttg cttgaactcg ccatggtgac gtgaaacatc gacacggata
     1501 ttgagttggc cttgctcagc ggcctgtgac agggtgtgca catcttctat caacaattta
     1561 aagttttggc gtacctgctc aatactttga ttgatataac tgcgctggcc ggggaaggtt
     1621 tccagtggct ggtgaaagtc gccctggcca aaggcggtca ccacttgcag agatttggtt
     1681 tgtgcgagga tatgctcctc caccatctgg ttaatgccgg tagtcagttg ctggtaggca
     1741 ccatgaaatg gctggctgtc tatgcggtag tctatcatgc cggcacggtg ttggctggac
     1801 atctcattta atgattcaac aatctgttga atggtcgatt gcatacggtg catttcacgc
     1861 agcagttttg cggtttcatc gttgcctgtc ggtgcttctt gcgcaatgaa ctcaccattg
     1921 gcaatgtgct tggacaaggt caatgcctgg ttgagtgggg tcgtgataga gcgcatcatt
     1981 ttccaggcaa tagcaatcgc cagcaccaca gcgaccaata aagccgtttc aatttgtttg
     2041 ttggcgctag cgattgtgga ggcgaactgg ctggaagcat cggtaacccc ggcaatattc
     2101 atatccatca gcaaacgcag cgaatggttc aaaggcttgt cagccccttt gaccgttgaa
     2161 tcaatctcgg tcacgctcat gccatcattt ttcagcgctt cacaacgttt aatggcatcc
     2221 cggtaagtgg tgagggcgag cgcgatatta cctaaaattt cctgttcttg tgcactgatt
     2281 tccccggtgg cctgataagc ggccatggct tgttcaatgt cactcatgct gtgttcaaac
     2341 ttggtggctt cacggccgcc gcgcaagaca taatttttaa aatgatgaat cgcatcgccc
     2401 aagcctttgc tggcgttttc aatttgtgtt cttttgacca gttgtacgtt ctcaaaatgt
     2461 tgccaggttt ggtgaatgtg gttgaagccg cgccaggcga tgacaaaaat aaaagtggta
     2521 aacaggataa gcagcgcgta acccaaaaat aaccgggtgc ctacattcaa acgagcgatg
     2581 gggtttttca tacaattcct caggataata cttggtaagc cagcaacatt gcgttgcctt
     2641 gcgcgtcacg ttttgtggcg cacaactgat ttacgtaaaa taaattggta aatgaagcgt
     2701 tttaataaaa tcttcacaaa agattcatat gccgactcgg gacagtcagt aaaaggcagg
     2761 gatggctgtg gcaggaatac gttgtatgat aggcatcaaa taaacaatga agcggtaatg
     2821 actgctgatg caatggatat tggattgaat ggctgaacat aaaaattaca ttacgccgga
     2881 aggttacgcc gcactggaaa aagagtttca atacctaatc aaggtcgacc ggccggaggt
     2941 ggtgcgtgta gtcagctggg ccgctggtaa tggtgaccgt agtgaaaatg gtgactacat
     3001 ttacggtaaa aaacgcctgc ggcaaattga ttcgcgcatc cgttttttga ccaaacgcat
     3061 ggatgctgcc gagattgtct acagcaacac gcagaccaat accgaaaaag tctatttcgg
     3121 cgcctgggtg acgctattca atttaaatga tgatagcgaa atgactatcc gtattgtcgg
     3181 ccaggatgaa ctggatccag cacaaggcta tatttcctgg gtatcgccga tggcaaaagc
     3241 cttgttggga aaaaatctcg gcgatacgat ccgtgtcatt acgcctgccg cagaaaccga
     3301 atatgaaatt atcgatatcc gctatcaata aaaccctgta aagattaggg tgtggacttg
     3361 aactaattta gttttaccct tatcttagcc ctatatgcgt aaacgcctgt gcctgttcct
     3421 gatatgctgg tttccatgct ttgtcatggc agccaatgtc atgtccatgc aaatggcgct
     3481 ggctcagcac gctttaccaa acatggttca agtgcaatcg gcagagatgc cttgccatca
     3541 catggcagat gcatcccacg aaacacaaca taccacagag caacacgccc ccggcaatca
     3601 tagctgcaca gtctgtggtt tttgcatggt tagtaccggg gtggcacatt tagacctgtt
     3661 ccccagtatt tcaatcattg tacaaagtgc tgctgcgccg ttatatgtag cggaccctgt
     3721 acattcacaa acctacccac ctgccatcaa accacccata ttcggctaac actcaggata
     3781 actccgtcta gtgatttcag agacggcgtt gtgacagttt gttagattga atatggaggt
     3841 tttatgttta agcaacatca cgttgcacct tttaatattt cacttgttag tagttcactt
     3901 ggcgccttac ttttaggcca gattgccttg agtgcccacg cagcggcgct gatgggcttt
     3961 gaggatagtg ccatgttaat gagtgaaatt gggcgctata accaggaatg gatgttgaat
     4021 tactcgccta cttttgggca tgctattggc gtcgagcaaa tgcgtatgtc aggcaagggt
     4081 gagcgcgcaa ccactatttc cggtatcaat tacactggca tcatcaaacg ctggaacctg
     4141 cctgcggctc aagccaatgt ctggttcaac ggtagtgtgg gtgaagcacg cgggcagtat
     4201 gacggcttcg cctacacacc cagcttgcag tttgatgtcg aatccacccg gctttatttt
     4261 ctggccaaag cccgcatgat cagggcacca catatgaact ttgacactgc cgcggtgcag
     4321 gccgggtttt ctttttacga aaccggtttt aacgacacgc agccatggtt cgtcatcgag
     4381 gccaaaacca tgcgcaacaa tgaccccggc ctgcaggtga cgccggcatt gcggttgatt
     4441 aacaaaaact actttcttga atttggtgta accaacccat ggcaaggcga aggttttgcg
     4501 ccgcgcttga atgccatgtt tgtgttttaa ctgattttat tttttaaagg atactgaaat
     4561 gaaaaaatta tggattgctc tgatgttgtc agccatgacc cctgcggctt ttgccgtgac
     4621 caccattaag gccgaggtca atggcatggt atgtgccttt tgtgccaaag gcatagagaa
     4681 aaaactcaat gccttgccgc aaaaacaagc tgcttttgta gacctcaaga gccgcgtggt
     4741 agcactgcag ctaaaagagg gccaggatgt ttcaaatgag gcattcagca aagtgattga
     4801 agatgccggt tattcagtca gtaaactcga gcgtgtcaac cagacggtgg cggtgataca
     4861 ggcggatgtc gccaacactg agaaggcggg cggcaaatga tggcagcaga aatcaatcat
     4921 ctacgggcca ataagcggct gaatctgctg tcattattta ccagtgggtc gacgctgatc
     4981 tgttgcgcct taccggcgac actggtggcg attggttcgg tcgcgacctt aaccagcctg
     5041 atcagtcatt ttccacagtt gatctggatc agtgagcata aaccactggt gtttggtctg
     5101 gcgggagcca tgttgctgct ggcgggttgg ctacaatggc gtgcacgctt gttaccttgt
     5161 ccggcagatc cacaactggc cacgttgtgt actaaaacac gacggcaaag cctgtggatt
     5221 tactgggtct cggtgatgat attcctgatt ggtgctttct ttgccttcat tgccccattg
     5281 ttgattgaat agagtactta ataaaacacc ttgcatatga aaaaagccga ctgttcagtc
     5341 ggcttttttt atgaccgcta gcgcttattt tggggttgcc ggcggttgct caggctcagc
     5401 cacaaatatc tcaacacggc ggttttttgc acggtttgct tcagtatcat tagcggcgat
     5461 aggctcatgt gagccgcggc catcaatggt gatacggctg attgcaatgc cgcgttcgac
     5521 caggtaacta cgggcgctgg cggcgcgatt cacggacaat gggtcattga tggcatctga
     5581 accactgctg tcagtatggc cgacgatcgt gacacgggct gccgcattat tttgtaagcc
     5641 agccgcaaac ttatcgagga tagggcggaa gt
//
LOCUS       DATMSK010000273         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_70374,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000273 DATMSK010000000
VERSION     DATMSK010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70374"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /gene="epsF"
                     /locus_tag="VNZ84_09160"
     CDS             complement(<1..856)
                     /gene="epsF"
                     /locus_tag="VNZ84_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HWU67168.1"
                     /translation="MNLNQIVLILLARYRIVLITFVLTVFTVLVISLLQSKVYQATAS
                     VVLNYKGTDPVTGAVVPGNMALGYVPTQIDIINSQHVALKVVDELRMAENENVKAKFE
                     EDTGGRGDLRFWLADLLLKKLTVTPGRDSSVIEIMFEGSDPDFVALVANAFANAYQET
                     NVELKVEPAQKAAEYFGAQIQALRDNLSQAQSKLSKYQRENGYTSSDERMDVEASRLN
                     WLSEQLVAAQGQSIEADSRSGGSRRNALESPDVVSNPIIQNLKINLATAESKLAELSQ
                     RLDKNHPQY"
     gene            complement(866..1903)
                     /locus_tag="VNZ84_09165"
     CDS             complement(866..1903)
                     /locus_tag="VNZ84_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HWU67169.1"
                     /translation="MNIIATLLFVTAFNAVAAEEYALGAGDVLRIQVFQNPDLTTEAR
                     VSESGEITFPLLGSVNVGGSGIGQAERKIAQGLVQGGFVQKPQVNIVLLQVRGNQVAV
                     LGLVNRPGRFVIETFDTHLSEMLATAGGVLSAAGSGKAIITGQRDGQAFRREVDLAEL
                     FLRDKKEADLLVAKGDVIYIVPGNQVSILGQVNRPGRYALEEAQMKLSDALAMAGGVN
                     VSGADSAILEGVRDGQKFHKVVDIAAIYLDENSDMNPDVLAGDSIYVHRAPVFYIYGE
                     VQRPGSYRIERSMTVMQALAEGGGPTPRGTQRGLKLHRKNASGVVEKTAPALDELVQS
                     GDVLYVKESLF"
     gene            complement(1968..2882)
                     /locus_tag="VNZ84_09170"
     CDS             complement(1968..2882)
                     /locus_tag="VNZ84_09170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWU67170.1"
                     /translation="MTFIRKAVFAAMVVAVAACGKSEEKASSGQVAAIVDGKEISVLQ
                     LNQAMKNMPNITAENADAVRKQLLDRLVDQQVILNKALEEKVERSAEVISSIEAAKRE
                     IITRAYLTSMVSSRVKPSPQQVKAYFDGHPELFSARKVYNLQDISLPAGTEITDELKA
                     LVERSKGLQEVAEWLKNQNIKFGASSYSRPAEQVATDALSVLKNQKDGAVMILSMDGN
                     PHIVKLIGAIQSPMPYDQAKPLIQTFLVNSEGQKLIKSELARLKEKSKIEYVGAFAKS
                     AGTEVAKPEAKPAEKNNSHIEKGVVGLD"
     gene            complement(2934..4181)
                     /gene="epsL"
                     /locus_tag="VNZ84_09175"
     CDS             complement(2934..4181)
                     /gene="epsL"
                     /locus_tag="VNZ84_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated polysaccharide
                     biosynthesis outer membrane protein EpsL"
                     /protein_id="HWU67171.1"
                     /translation="MYASKKLIPFNRCLMVVNVAVFMSMVGTASADQQDVVNFTLNHA
                     VTYDDNLFKLDRNESPTTTFGKSQRWEMINATTYGVSINKPYSLQRFKFDVNQTSYRY
                     QNYNFLNFDGTNYDIQWNWSLTPYLKGNIVFDRQQLPNTFANITSINPLTTNTRTVKS
                     NRFDIDWSPQGNWHLLGGVTDLTVTTDNSSPTQNNIDFSFEQTSARAGVRYDLTSGSN
                     LEYGASTANGKYGQSTLNEVLFTDQGYEEKRQEFKFNWVLSGKSKLNLSATYLDRNND
                     NFSQRDVSGWIGAVNYSWDVTGKTRINFIASTDLFPTQAISYTYVRQNLIAIRPIWSV
                     TSKVIVRGDLTLRERAFEGFAPAPPAEDRHDRTRLASISASWEPRRFVSLGVMLKREV
                     RDSNLINDDYVSNSAMINAGLTF"
     gene            complement(4202..>4740)
                     /locus_tag="VNZ84_09180"
     CDS             complement(4202..>4740)
                     /locus_tag="VNZ84_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWU67172.1"
                     /translation="QIIESFCLSMYSDLIHLGGADKYVDEVLEIINSIKLLDDFSADE
                     VRYLCAYMQCYAAPRDYSLLVEGAGGDYLLLILSGGVDVYKNDASAEGRHMVARLGIG
                     DTIGEMSLIDGNPRSASCVTTSPTDFAVFSRSSMQELLVNMPKLGNKILIKLLQLMTE
                     RLRDTSNRYPDGAFFSLV"
BASE COUNT         1199 a         1219 c         1060 g         1262 t
ORIGIN      
        1 gatattgcgg gtgattttta tccagacgtt gagataattc agcgagcttg gattcggccg
       61 tagccaaatt gattttcaga ttctgaatga tgggattact caccacatca ggtgattcca
      121 gcgcatttct ccgacttcca cccgagcgtg aatccgcttc gatggattgc ccttgcgcag
      181 ctaccagttg ctctgagagc cagttaagac gcgatgcttc aacatccatt ctttcatcgg
      241 agctggtgta tccattttca cgctgatatt tggaaagttt ggattgtgct tgtgaaaggt
      301 tgtcacgaag agcctgaatc tgagccccaa agtactcagc ggccttttgt gctggctcaa
      361 ctttcaactc gacgttggtt tcctggtacg catttgcaaa agcattggcg accaaggcta
      421 caaagtcagg gtccgatcct tcaaacataa tctcgatcac gctactgtcg cgaccaggcg
      481 tcacagtgag tttctttaac agaagatccg ccaaccagaa gcgcagatca ccacggccac
      541 ccgtatcttc ttcgaacttg gctttgacgt tctcgttttc ggccatacgc aactcgtcaa
      601 ccaccttgag tgcgacgtgt tggctgttga tgatgtcaat ctgtgttggg acgtagccca
      661 gagccatgtt cccaggcacg acagcaccgg tgacggggtc tgtgccttta tagttgagca
      721 caacagaggc agttgcctga taaaccttgc tctgcaataa gctgattaca agcacagtaa
      781 ataccgtcag cacgaatgta atcaatacaa tacggtaacg cgccagtaat atgagtacga
      841 tttgattcaa attcatccga gattcctaaa acaggctttc tttaacgtaa agtacgtcgc
      901 cagactgcac taactcatct aacgcaggtg cggttttctc gacgacacca gatgcatttt
      961 tacgatgcag tttcaagccg cgttgcgtac cgcgtggtgt aggtccacca ccctcagcca
     1021 atgcttgcat gacggtcatg ctgcgctcta tacgataaga gcctggacgt tgcacttcac
     1081 catagatata gaacacaggt gcacggtgta cataaatgga atcaccagca agcacatcgg
     1141 ggttcatgtc cgagttctca tccaggtaga tggccgcaat atcgacgacc ttatggaatt
     1201 tctgaccatc acgcacccct tccaggatgg cagagtctgc gccgcttaca ttaaccccac
     1261 cagccatagc cagcgcgtct gacaatttca tctgagcttc ttcaagcgca tagcggcctg
     1321 gccgattcac ttgcccaaga atggaaacct gattgccagg tacgatatag atcacgtcac
     1381 ccttggctac gagcaggtca gcttctttct tgtcacgcaa gaaaagctca gccaggtcga
     1441 cttcccggcg gaaagcctgc ccgtcacgct gaccagtaat gatggccttg ccactgccag
     1501 cagcagaaag cacaccaccg gcggttgcga gcatctcact gaggtgagta tcgaacgtct
     1561 ctatgacaaa acgaccagga cgattaacca ggccaagtac tgccacctga ttaccacgta
     1621 cttgcagcaa tacaatattg acttgcggtt tttgcacgaa accaccctgt acaagtccct
     1681 gagcaatctt gcgctccgct tgaccaatgc cggagccacc cacatttact gagcccagca
     1741 gaggaaaagt aatttcgccc gactcagaaa cgcgcgcctc tgtcgtgaga tcagggttct
     1801 ggaaaacctg gatacgcaac acgtcgcctg cacctaaggc atactcttcg gctgccaccg
     1861 cgttgaatgc agtaacaaaa agaagagttg cgatgatatt catgcaccat ttcatcatga
     1921 tatttccctg tttgattatg gtttaattaa gtgatagtga gcggagttta gtccaggccc
     1981 acgacccctt tttcgatatg gctgttgttt ttctctgcag gtttcgcttc gggttttgct
     2041 acctcagtgc ctgcgctttt tgcaaaggct ccaacatatt caatcttgga tttttctttc
     2101 agtcttgcca gctcagactt gatcagcttc tgcccttccg agttcacaag gaaagtctga
     2161 atcaagggct tggcctggtc ataaggcata ggagactgaa ttgcgcctat gagtttcaca
     2221 atatgcggat taccgtccat ggaaagaatc atgaccgcac catctttctg attcttgaga
     2281 acactaagcg catcggtcgc cacttgctct gcagggcgac tgtaactgga agcaccaaac
     2341 ttgatgttct ggtttttgag ccactcggca acttcctgca aacccttgct acgctccacc
     2401 agggctttca actcatcagt aatctcggtt cctgctggca gggaaatatc ttgcaggtta
     2461 tataccttac gggccgaaaa cagctccgga tgtccatcaa aataagcttt cacttgctgc
     2521 gggcttggct ttacacgtga actcaccata ctggttagat aagctctcgt aatgatttcg
     2581 cgcttggccg cttcgatgga tgaaatcact tcagcacttc gctcaacctt ttcctccagc
     2641 gccttgttca agatgacctg ttgatccacc aggcgatcaa gcaactgctt gcgcacggca
     2701 tctgcattct cggctgtgat gttaggcatg tttttcatgg cctgattaag ttgcaggacc
     2761 gaaatctcct tgccatcgac aatcgcggcc acctggcccg aggacgcttt ttcttctgat
     2821 ttcccgcagg cggctacagc aaccaccatg gcggcaaaca ccgcttttct tataaaagtc
     2881 attattgctc caagttcacg atatgcgcgg ccatgcattg gtcggcttat tatttagaag
     2941 gtcaaacctg cgtttatcat cgcgctattt gaaacatagt catcgttgat aagattcgag
     3001 tctctcactt cccgcttcag cattactccc aggcttacaa aacggcgcgg ctcccaagac
     3061 gcgctgatac tggccaagcg cgtgcggtca tgccgatcct ctgcaggagg agccggtgca
     3121 aacccttcaa acgcacgttc acgcaaggtc aaatcccctc ttactatcac ttttgaagtg
     3181 accgaccata tcggtctgat cgcaatcaga ttctgacgca cataggtgta tgagatggct
     3241 tgcgtgggga ataaatcggt gctggcaatg aagttgatac gtgtcttccc ggttacatcc
     3301 caactgtagt ttaccgcccc aatccagcca cttacgtcac gttgactaaa attgtcatta
     3361 ttgcggtcaa gataggtagc acttaagttc agttttgact tccctgatag cacccagttg
     3421 aatttgaatt cttgacgctt ttcctcataa ccttggtctg tgaaaagcac ttcgttcaga
     3481 gttgactgac catatttacc attggcagta ctagcaccat attccagatt cgaacctgaa
     3541 gtcaggtcat atcgaacacc cgcgcgcgcc gatgtctgct caaaactgaa atctatattg
     3601 ttctgagtag gcgaagagtt gtcggttgta acagtaagat ccgtcacgcc accgagcaaa
     3661 tgccagttac cctgcggact ccaatcaatg tcaaagcggt tgcttttcac tgttcgagta
     3721 ttggtagtaa ggggattaat actcgtaata ttggcgaagg tatttggcag ttgttgacga
     3781 tcaaatacga tgttgccttt gagataaggg gtcagtgacc aattccactg aatgtcgtaa
     3841 ttggtgccgt caaaattcaa aaagttatag ttttggtacc ggtaggatgt ctgattgaca
     3901 tcaaacttga atcgttgaag gctgtaaggc ttgttgatgc tgacaccata ggttgtcgca
     3961 ttgatcattt cccaacgctg gcttttacca aaggtggtag tcggactctc attgcggtca
     4021 agcttgaaca agttatcgtc ataagtgacc gcatgattca gtgtgaagtt aacaacgtct
     4081 tgttgatccg ccgaagcagt tcccaccatt gacataaaaa cggcaacatt caccaccatc
     4141 aaacaccgat taaacggtat taactttttg gatgcgtaca aaattaactc tcgcagcgtg
     4201 tttagaccag actaaaaaaa gcaccgtcag gataccggtt agaggtatct cgcaacctct
     4261 ccgtcataag ctgcaataat ttaatcaata ttttgttgcc aagttttggc atattcacca
     4321 gcagttcttg catactgctg cgtgaaaaga ctgcgaaatc tgtgggggat gtggtaacac
     4381 aactggcgga tcgaggattg ccatcaatca gggacatttc ccctatggta tcgccaatac
     4441 caagacgagc caccatatgg cgaccctctg cggatgcatc atttttgtac acgtctaccc
     4501 ccccacttaa tattaatagc aggtagtcgc caccagcacc ctcgactaat aacgaatagt
     4561 ctcttggcgc tgcatagcac tgcatgtagg cacatagata gcgaacttca tcggcactga
     4621 agtcatcaag cagcttgatg ctgttgatta tttccagcac ttcatctacg tacttatctg
     4681 ccccgcctaa atgtattaaa tcagagtaca tggataaaca aaaactttca atgatttgtg
//
LOCUS       DATMSK010000274         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_186984,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000274 DATMSK010000000
VERSION     DATMSK010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186984"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..531)
                     /gene="rplS"
                     /locus_tag="VNZ84_09185"
     CDS             complement(142..531)
                     /gene="rplS"
                     /locus_tag="VNZ84_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWU67173.1"
                     /translation="MADIIKMLEQEEIARLNKTIPSFAPGDTVIVGVNVVEGNRKRVQ
                     AYEGVVIAKRNKGLNSSFIVRKISSGEGVERTFQTYSPQIASIEVKRRGDVRRAKLYY
                     LRERSGKSARIKEKLVAREKEIAADNS"
     gene            complement(565..1314)
                     /gene="trmD"
                     /locus_tag="VNZ84_09190"
     CDS             complement(565..1314)
                     /gene="trmD"
                     /locus_tag="VNZ84_09190"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWU67174.1"
                     /translation="MQIEVISLFPEMFAALTEYGITSRARQRGLYQLTLTNPRQYTQD
                     VHHTVDDRPYGGGPGMVMLAEPLTAAIGEAKARLHAQGVMPKVIHLSPRGKPLTHEKV
                     LQLKELKGLVLLASRYEGVDQRVLDTQVDEELSIGDYVLSGGELPAMVLLDAMIRQLP
                     GALGDADSAQEDSFVNGLLDYPHYTRPEEFAGLKVPEVLLSGHHEKIRQWRLQQSLLL
                     TRAKRPDLLAERPLTKEEARLLKAADTSSVE"
     gene            complement(1333..1884)
                     /gene="rimM"
                     /locus_tag="VNZ84_09195"
     CDS             complement(1333..1884)
                     /gene="rimM"
                     /locus_tag="VNZ84_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915191.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWU67175.1"
                     /translation="MAFEGMVVMGRIVAPYGVYGWVKVVPDTEYLDGLLEYETWWIGK
                     APNWREVTVEQAKIHNDVLLIKLAGIADRNVAFSLKGQHIAVPRDALPETEEGEYYWS
                     DLIGLSVSNTEGVVFGKITQVFETGANDVLVVREDQPTSVKGKDGKVREEYRERLIPF
                     TSEAVPEVDIQAGTVLVDWDADF"
     gene            complement(1907..2347)
                     /gene="rpsP"
                     /locus_tag="VNZ84_09200"
     CDS             complement(1907..2347)
                     /gene="rpsP"
                     /locus_tag="VNZ84_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWU67176.1"
                     /translation="MVVIRLSRGGSKKNPFYSVVVADSRNRRDGRFIERVGFYNPSAR
                     AGAEGLRIDEARTQYWVENGAQLSDTVARLVKFHAKGVEGMAAVKAKEAAKADAAKEK
                     AIAEAAAKLKAEADAAAAEAAAKAKAEADAAAAEAAAAEAPAAE"
     gene            complement(2429..3130)
                     /gene="queC"
                     /locus_tag="VNZ84_09205"
     CDS             complement(2429..3130)
                     /gene="queC"
                     /locus_tag="VNZ84_09205"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWU67177.1"
                     /translation="MATPQQPKAVILLSGGLDSSTVLAIAKSQGYDCYCLSLDYHQRH
                     NVELLAAQRVAQALGAIEHKTVQLDLSLFGGSALTDSNIAVPEHETEGIPVTYVPARN
                     TIMLSLSLAWAEVLQARDIFIGVNALDYSGYPDCRGEYVKAFQQMANLATKAAVEGQT
                     ITVHAPLIDMTKADIIRTGHALGLDYGLTVSCYQADAQGAACGVCDSCRFRKAGFAQA
                     GVVDPTPYKNTSSGK"
     gene            complement(3133..3774)
                     /gene="queE"
                     /locus_tag="VNZ84_09210"
     CDS             complement(3133..3774)
                     /gene="queE"
                     /locus_tag="VNZ84_09210"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HWU67178.1"
                     /translation="MTTLRIFEIFYSLQGESSRVGLPTIFIRLTGCPLRCGYCDTEYA
                     FKGGNNMTLDEIMQQIAAYGTRYVCVTGGEPLAQKPCIELLTRLGDAGYEVSLETSGA
                     MDITAVDPRVKVIMDIKTPGSGEVEKNRWENLPLLKAGDEVKWVICSREDYDWAKHML
                     QTRPFPAQCDQLFSPSFHDVSGETLASWILQDHLKVRMQLQLHKILWGEKQGV"
     gene            complement(3859..4728)
                     /locus_tag="VNZ84_09215"
     CDS             complement(3859..4728)
                     /locus_tag="VNZ84_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HWU67179.1"
                     /translation="MQASNLRLSAALIALGLAVTTASHAALFDDTEARKRILELENKQ
                     TSDHDAQQKAITDLTRTQQSLDKRVQSLEALINGKGLLDMQNQLEQIRQDVAQLKGDL
                     EVVTHQLEDMRTKQNAAYTDLDTRVRKLETVAQSVTGGQSGTPSANSMNGNGGEAKTD
                     VSQQELSAFAEAESLNQAGKYKEAFNAYDAFLKSYSGSKLVPDALYGMGYVQFALKSY
                     KSSAATQQKFLDSYASHALAPNAMLNLANNQIQLGQIPAAKKTLKELIAAYPSAEVTP
                     SAQKRLKVLESIK"
     gene            complement(4715..>5173)
                     /gene="pal"
                     /locus_tag="VNZ84_09220"
     CDS             complement(4715..>5173)
                     /gene="pal"
                     /locus_tag="VNZ84_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HWU67180.1"
                     /translation="SVTRPMSKSQDAGADSSANTDKINIKTVDVDTSKDELSAAELAK
                     RQVFFDYDSDAIREEYKALVANHAKYLVAHPQTKVVLQGNTDERGTHEYNLALGQRRS
                     VAVKNALNVLGVNDSQIETVSYGEERAKQNCADDGCFQQDRRVDIVYAGQ"
BASE COUNT         1226 a         1425 c         1153 g         1369 t
ORIGIN      
        1 gaattaaagc atcgagtgca tcatcaagac ttttcatata gttatttaac tgtaatcctg
       61 cctaagctgc aactctctaa acattgttaa tgacaataaa aaagcccttc agttgaaggg
      121 cttttttgtg caacgcttga attaagagtt gtctgctgcg atttcttttt cgcgtgcaac
      181 cagtttctct ttaatacgtg cagatttacc tgaacgctca cgcaagtagt acagtttagc
      241 gcgacgaacg tcaccacggc gtttcacttc gatagaagcg atctgtgggg agtaagtctg
      301 gaatgtacgc tcaacacctt caccagaaga gattttacgt acgatgaagg atgagttcag
      361 acctttgtta cgtttagcga tcactacgcc ttcgtaagcc tgtacacgtt tacggttacc
      421 ttcaaccacg tttacaccta cgatcacggt gtcacctggc gcaaagctag ggatggtttt
      481 gttcaagcga gcaatttctt cttgctctaa cattttgata atgtctgcca tgtcagttcc
      541 ttttaattgt aaagtgactc gttcctactc cacgctgctt gtatcagcag ccttgagtag
      601 cctagcctct tctttcgtca acggcctttc agccaataaa tccgggcgtt tagcccttgt
      661 tagcagcagc gattgctgta atcgccattg tctaatcttt tcgtggtgtc ctgatagcaa
      721 tacctcaggc accttcaatc ctgcaaactc ttctgggcgt gtgtagtgcg gataatcgag
      781 caggccattc acaaatgaat cttcctgcgc cgaatcagca tcgcccaacg ctcctggtag
      841 ctgtcggatc atcgcatcca gcaataccat ggcgggcaac tcaccaccgc ttaacacata
      901 atcgccgatg gagagttcct catccacctg ggtatccagc acacgctggt ctaccccttc
      961 atagcgactg gctaacaaca ccagcccctt taattctttt agctgcaaca ctttttcgtg
     1021 tgtcaatggt ttgcctcgtg gtgataaatg tatcaccttg ggcatgactc cttgcgcatg
     1081 taaccgcgct ttcgcctcac caatcgcagc ggtcaacggc tcagccagca tcaccatccc
     1141 aggaccaccg ccatacggcc ggtcatccac cgtatgatgc acatcctgcg tatactgccg
     1201 cggattggtc agggttaact gatacaagcc gcgctgcctg gcacggctgg taatgccata
     1261 ttccgtcagt gctgcaaaca tctcaggaaa caaactgatg acttcaatct gcatcatgag
     1321 atcagctttc ttttaaaaat cggcatccca gtccaccagc acggtgccag cctgaatatc
     1381 cacctctggc accgcttcac tggtaaaagg aatcaagcgc tcacgatact cttcgcgtac
     1441 tttgccgtct ttacctttga cactggtggg ctgatcttcg cgcacaacca gcacatcatt
     1501 ggcgccagtc tcaaacactt gcgtgatttt gccaaacacc acaccttcgg tattgctgac
     1561 acttaaccca attaggtcag accagtagta ctcgccttct tccgtctcag gcaatgcatc
     1621 acgaggaacc gcaatatgct gacctttaag gctaaatgcg acattcctgt ccgcgatacc
     1681 agccaactta atcagtaata catcattgtg gattttggct tgttcaacgg tgacttcacg
     1741 ccaattaggt gctttaccta tccaccaggt ttcgtattct agcaacccgt ccagatactc
     1801 ggtgtcaggc actactttca cccagccata aacgccatat ggggcgacga tgcgccccat
     1861 cacaaccatg ccttcaaacg ccatctgaat tcaggcgtcg aaacaattac tcagcagctg
     1921 gtgcttcagc ggcagcagct tctgcagcag cagcgtcagc ctctgctttg gccttagcgg
     1981 cagcttctgc agcggcggca tcggcttcag ctttcaactt tgcagccgct tcagcgattg
     2041 ctttttcttt tgctgcgtca gccttggcag cttcttttgc cttgacggca gccatacctt
     2101 caacgccttt agcgtggaat ttaaccagac gcgctacggt gtcggacagt tgtgcaccgt
     2161 tctctaccca gtattgtgta cgtgcttcat cgatacgcag gccttcagcg ccagcgcgtg
     2221 cagatggatt gtagaaacca acgcgctcga taaaacgacc gtcacgacgg ttgcgggaat
     2281 ctgccacaac tacgctgtaa aacgggttct ttttagagcc accacgtgat aaacgaataa
     2341 cgaccataat cttgttctct taaaattgaa tttctttgca gaaaggcgcg gattatacta
     2401 gattttccaa acgcgtggca agtctttttc acttgccgga tgacgtgttc ttgtatggtg
     2461 tcggatcaac tacgcctgct tgtgcaaaac ctgctttacg gaaacggcag gagtcgcaca
     2521 caccgcatgc ggcgccctga gcatcagcct gatagcacga aacagttaag ccataatcca
     2581 gccccaaggc atgtccggtg cggataatat cggccttggt catatcaatc aacggcgcat
     2641 gcacagtaat ggtttgccct tctacggcgg ctttggtggc cagattggcc atctgctgga
     2701 aagctttaac gtactccccg cgacaatccg gatagccaga ataatccagc gcattcacgc
     2761 ctatgaaaat atccctcgct tgcaacacct cggcccatgc caaagataaa gaaagcataa
     2821 tcgtatttct cgctggcacg taagtgacag gaatgccctc ggtttcatgc tcaggcacag
     2881 caatattgct gtcggtaagg gcagagccgc caaacaacga caaatcaagt tgcacggttt
     2941 tatgttcgat tgcacccagc gcctgagcca ctctctgcgc tgccaacaac tctacattat
     3001 gtcgctggtg gtaatccaga ctcaagcaat agcagtcata tccctgcgat ttggcaatcg
     3061 ctaacacagt gctggaatcc aagccaccag acaataaaat cactgctttt ggttgttgag
     3121 gggttgccaa acttatactc cttgtttctc gccccagagt atcttatgca attggagctg
     3181 catgcgtacc ttgagatgat cttgcaaaat ccagctcgcc agtgtttcac cactgacatc
     3241 atgaaaactt ggcgagaata gctggtcaca ctgcgccggg aaaggtcggg tttgcaacat
     3301 gtgcttggcc cagtcatagt cctcgcggct gcaaataacc cacttcacct cgtcccccgc
     3361 ttttagcaaa ggcaggtttt cccaacggtt tttttccacc tcgccagagc ccggggtttt
     3421 gatgtccata atgactttga cgcgcggatc caccgccgta atgtccatgg caccactggt
     3481 ttccagggac acttcatacc cggcatcccc caaacgcgtc aataattcaa tacatggctt
     3541 ctgtgccaat ggctcacccc ctgtgacgca cacatagcgg gtgccatagg cagcaatctg
     3601 ctgcatgatt tcatccagcg tcatattgtt gccgcccttg aatgcatatt cggtatcaca
     3661 atacccgcag cgcagggggc aacccgtcag ccggataaaa atggtgggca ggcccacgcg
     3721 tgagctttcg ccctgcaagg aatagaaaat ctcgaaaatt ctgagtgtgg tcatgggaat
     3781 atgaaaataa aacgggcaga tattgctatc tgcccgctat tatcgccgaa ataaggtaat
     3841 ttcaataacg aaaatctgct acttgatact ttccagtact ttcagtcgtt tttgtgcact
     3901 tggcgtcact tctgccgatg ggtaagccgc aatcaactct ttgagggttt ttttggcggc
     3961 cggaatctgc cccagctgaa tctggttatt ggccaagttc aacatggcgt taggcgccag
     4021 cgcatgcgat gcatagctat ccagaaattt ctgctgggta gccgcagagg atttgtagct
     4081 cttcagcgca aactgcacat aacccatacc atacagtgca tcagggacta atttgctccc
     4141 tgagtagctt ttcaaaaagg catcataggc attaaaggct tctttgtact tgccagcctg
     4201 gttgagggac tccgcttcag caaacgcgct cagctcctgc tgcgagacat cagtcttggc
     4261 ttcaccaccg ttaccattca tgctattggc tgaaggtgta ccggattgac caccggtgac
     4321 agactgcgcg actgtttcca gtttgcgtac gcgcgtatcc aaatcggtgt aggcagcatt
     4381 ttgcttggtg cgcatatctt ccaactgatg cgtcaccact tccaaatcgc ctttcaattg
     4441 ggccacatcc tgccggatct gttccagctg gttttgcata tccagcaaac ccttgccgtt
     4501 gatcagcgcc tccagactct ggacacgctt atccagcgat tgctgggtac gcgtcaaatc
     4561 agtgatcgcc ttttgttgcg catcatgatc tgaggtttgc ttgttctcaa gctcaagaat
     4621 gcgttttctg gcttcagtat catcaaacaa agccgcgtgg ctggctgtcg taacagccag
     4681 ccccaaggcg atcaacgcag cagataaacg caaattactg gcctgcataa acgatgtcta
     4741 cgcgacgatc ttgctggaag caaccgtcat ctgcgcagtt ttgtttagca cgctcttcac
     4801 cgtagcttac agtttcgatt tggctatcgt tcacacccag cacattcaaa gcatttttca
     4861 cagcgactga acgacgctga cccaaagcca ggttgtactc atgtgtacca cgctcatcgg
     4921 tgttaccttg cagcaccact ttagtttgtg ggtgagcaac cagatacttg gcatgattag
     4981 cgaccagcgc tttgtattct tcgcggattg catcgctgtc ataatcaaaa aacacctggc
     5041 gttttgccaa ctctgccgca ctcaactcat ctttgctggt atccacatct actgttttga
     5101 tgttgatctt atcggtgttg gcagagctgt cagcgccagc atcttgtgat ttgctcattg
     5161 ggcgagtcac gct
//
LOCUS       DATMSK010000275         7484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_163102,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000275 DATMSK010000000
VERSION     DATMSK010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163102"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VNZ84_09225"
     CDS             <1..1036
                     /locus_tag="VNZ84_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWU67181.1"
                     /translation="WIIGGVLNTPIETANKRDYRTDKAMLLAKAADLRVADSIWLIRS
                     RLKAALLEAYSAQESVKLLEQHITIQTKINQLLEQQATVGEIALVEVTRARITLNQLK
                     IGLDTAHKRIADSRVMLATIIGIPVEGLPANVLQAEEWSSVPDLMQVPVQQMKRVALH
                     SRPDIVAALTDYDAAQSALQLEIANQYPNLSLAPGYTWDLGANYWVLGASLLQLPIFN
                     QNQGPIAEAEAKRKEMAQRFLALQLKVISDVDRANAGLQSIADKWTEVERQQQMQKKH
                     QEEVQANYDGGEADQITLLLAKIEDIATQRSALDVLIESHQAENGLEDTLRQPMHSVL
                     SANLQARADH"
     gene            1047..2105
                     /locus_tag="VNZ84_09230"
     CDS             1047..2105
                     /locus_tag="VNZ84_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWU67182.1"
                     /translation="MINRLQPSFLILLGLCLTACHAPDEETSTPSTIVSVHVANIQSK
                     TLSQYVDAFGLVEPEPALQGKPSASSKVSSPVAGLITRVLCEEGQYVHQGDVLFELDA
                     RSADEAVSKAEVAVEFAQKGFERKEALYANNNTSLKLYKDAEQQLETAKKDLSNARTQ
                     REILRIKAPLSGTISTIHYKIGESISVNTVMADLMDLKRLDVALKVPTTEVAAIHTGQ
                     QVEVFADDSASAKPYTGKVIFLSPQVDPTTDTVLVRASFASDARIRPGQFVSARIQTV
                     RLSDQLAVPQASVLHGDSGDYVMLVSGQIAKQYPVKIGIKDHGWVGLTDATLKPGMQV
                     VTEGSYGLPVQSRISIQQ"
     gene            2114..5257
                     /locus_tag="VNZ84_09235"
     CDS             2114..5257
                     /locus_tag="VNZ84_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU67183.1"
                     /translation="MPFDFPVKHRLTIVFLCVALCLAGLYAALQMPSSIFPQTNFPRV
                     VILLDNGVMPSNEMMATITRPVEESMKDIPGVENVRSKTGRGTAEVNVFFNWHTDMTQ
                     AELYVRGKLAMVQKTLPPTANYSVSRLTFSAFPVMGLSLTSDKHDVTQLWETARYTIQ
                     PKILRLKNVAKVGIVGGRVPEYHIVVDPLKLQALHLGISQVADLVARSNVIAPAGMLE
                     EDYKLYLTVTDGRIHGTEELENLVIAAPNPASAPGSLGGNPAATGIADHPVRIKDFAR
                     VERNKEPAFYEVTADGKNAVLMNVYSQPDGSTLDIAQAVKAELISINKSLPPGMKVQF
                     FYDQSELVADSMQSVWEAILVGLVLSMAILYLFLQNWGTTLVASIVIPVTVCFAMLGL
                     KVAGLGFNLMTLGGIAAAIGLVIDDAIVVVEIIHAKMQHGQQRLAAITEGLSEVFRPL
                     LASTFTPVVVFLPLAFLDGVPGTFFKALALTMVVSLLSSLLLAVTLIPAMASWLLSAE
                     QLTPASHQAAQHADEQGLFAKIQLFYTSHLNRALAKPKSLIFFSALLLSLSAYLYTLL
                     ENEFLPFMDEGGFVLDYHAPWGTSLTETNRQLLQAEQLIKSMPELEGYSRRTGVRLAL
                     GISQAHNGDFLVKLKKNRQRSTDEVITDLRQKLNTAVPGLHWEFAGILGDLIGDLTWS
                     PKPLELKLFSTDIKWLQKTAPLVQAKLDQIPGVVDSFDGLESTGPSIHVRVRSADAAR
                     FGLTSNDIQLAVSTAMLGQKASYVLEGDRVVNIRVRMPSEVTGQMDKLMQLPLRTADG
                     RTVQLKQVADLELVAGELEMQREDMRQLVAVTARLEHRDLGSAINEIQHKVAQDPVLA
                     KANIEFGGLYQQQHESFIKLTLVLLASLFLVFTVLLIQFRTFQAPISIVSGAILAMSG
                     TVIAWYVTGTSVNIVSLLGAIIGVGVVAKNGILMLDGVQKFHQAGLDLPASLLASGVS
                     RLRPVLMTSLAAALGLLPLAYGVGAGSDMLKPMAIAVIGALVLSVLLSLIATPVFYYL
                     LQRSSLKQKTYYPETEI"
     gene            5270..5941
                     /locus_tag="VNZ84_09240"
     CDS             5270..5941
                     /locus_tag="VNZ84_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HWU67184.1"
                     /translation="MHILIVEDEKKTAAFLQKGLTEHGYTVDVCHDGEAGLILANSVA
                     FDLMIFDIMLPLMDGLTLLKKIRANNQHTPVLFLSARDTLEDRVKGLELGADDYLVKP
                     FAFVEVLARVRSLLRRGVVLQPEQLRIADLTLDRGKHKAYRAGQLLDLTPKEFALLAL
                     FMQRPGEVLSRTLIAEQVWDINFDFDSNIIDVAIRRLRQKMDDAYSPKLLHTVRGMGY
                     VFESR"
     gene            5922..7316
                     /locus_tag="VNZ84_09245"
     CDS             5922..7316
                     /locus_tag="VNZ84_09245"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HWU67185.1"
                     /translation="MSLNLANLHTGRWSITARLTLLFSVAAALIQALLSVYLYSSLQH
                     TFLKSNRQFLLREMQVLNKIVQAQPSDVAHLAVEQREGLDLPNGKYYSRVLDAQQRII
                     SETPGMPDILGAGNAIEHAQGPSEQWIQRAGHQSYLVLSRTLSGQAATVTFQMALDTT
                     SQTHRLSEYQRTLLVAFLFGVLLSALAALFIARHGLKSLATMAKEVGGIGALSLTKHL
                     TVADYPTELVHLVLSFNHMLMRLNQSFEQLQQFSADLAHELRTPVNNLIGESEVALGQ
                     VRSDEDYQQLIASNLDEYHRLSQIIEALLFLARADNTEITAKKQPLNAAEQVIAVFSY
                     FEALAEERGIKLSCTGDAVVFADMQLLRRVLSNLIHNAIKFVAEGGEVSVLIDATDAA
                     TVITIQDNGAGIAAKHLSKIFDRFYRASASRTEHGTGLGLSIVKSIMTLHGGTVGIES
                     MIGTGTSVTLTFVN"
BASE COUNT         1846 a         1770 c         1981 g         1887 t
ORIGIN      
        1 ctggattatt ggcggtgtgc tcaatacgcc aattgagact gccaacaaac gagattaccg
       61 tactgataaa gccatgctgt tggcaaaagc ggccgatctg agagtggccg atagcatctg
      121 gctgattcgc agccgcctga aggcagccct gttagaggct tactccgcac aagagtccgt
      181 caagctttta gagcagcaca ttactattca gaccaaaata aaccagctac tggagcaaca
      241 agccacggtt ggcgaaattg cattggttga agtcacacgt gccagaatca ccctcaatca
      301 actcaagatt ggcttggaca cggcacataa aagaatagcc gatagccgcg tcatgctggc
      361 gacaatcatc ggcattccgg tggaaggcct gccagcgaat gtgctgcaag ctgaggagtg
      421 gtcatcagtg cctgatttga tgcaagtgcc tgtgcagcag atgaagcgtg tggcgctaca
      481 tagccgcccg gatattgtgg ctgcactcac cgattatgat gcggcacagt cagcactgca
      541 gctggagatt gccaatcaat atcccaatct ttcgctggcg ccaggctaca cctgggattt
      601 gggcgcaaat tattgggtgt taggtgcctc gctattgcaa ttgcccatct ttaaccagaa
      661 tcagggaccg attgctgagg ctgaagccaa aaggaaggaa atggcgcaac gctttctggc
      721 actgcagtta aaagtcattt ccgatgtgga tcgtgccaac gccggtttgc agtcaattgc
      781 agacaaatgg actgaagttg agcgccaaca gcaaatgcag aaaaagcacc aagaagaggt
      841 acaagccaat tatgacggtg gcgaggcaga tcaaattacc ctgttactgg ccaagataga
      901 agacattgcg actcaacgtt ctgcgctgga tgtgctgatt gaaagccatc aggctgagaa
      961 tgggctagag gatacgctac gtcaacctat gcattccgta ttgtccgcaa atttacaagc
     1021 cagagccgac cactgaaaga ccgcacatga ttaaccgact gcaaccctct tttttaattt
     1081 tgctgggatt gtgtttgact gcttgccacg cccctgatga agaaacctcc acgccatcca
     1141 cgatagtcag tgtgcatgtg gccaatatcc agagcaaaac attgtctcaa tatgtagacg
     1201 cgttcggcct ggttgagccg gagccggcgt tgcaaggcaa accttctgcc agctccaagg
     1261 tatcatcacc cgtggccggg ttgatcacaa gagtactgtg tgaagaaggg cagtacgtcc
     1321 atcagggcga tgtgttgttt gagctggatg ccagaagtgc cgatgaagct gtgtccaaag
     1381 ctgaagtcgc ggtggaattc gcccaaaaag gcttcgagcg caaagaggca ctgtatgcca
     1441 acaataacac ttcgctcaag ttgtacaaag atgcagagca gcaacttgaa acggcgaaaa
     1501 aagacttgtc gaatgcacgt acgcaacgtg aaatcctgcg tatcaaggcg ccactcagcg
     1561 gcactatttc aaccatacat tacaaaatcg gcgaatcgat cagtgtcaat actgtcatgg
     1621 ccgacttgat ggatttaaag cgcctggatg ttgcgctcaa agtaccaacc actgaagtag
     1681 cggccatcca caccgggcag caggtggagg tatttgccga cgatagcgca agcgccaagc
     1741 cttataccgg caaggtaatc ttcttaagcc cgcaggtgga cccgacaaca gacacggtcc
     1801 ttgtgcgtgc ctcatttgcc agtgatgccc ggattcggcc tgggcagttt gtcagtgcgc
     1861 gcattcagac cgtgcgcttg tcggaccaac ttgccgtgcc tcaagccagc gtgcttcacg
     1921 gtgatagcgg tgactatgtg atgttggttt ccgggcaaat cgccaaacaa tacccggtga
     1981 aaattggcat caaggatcac ggttgggttg gcctgacaga tgcaacctta aaacctggca
     2041 tgcaagtggt taccgagggt agttacggat tgcccgtaca aagccgtatc agcattcagc
     2101 aataaggaat cctatgccat ttgattttcc ggtcaagcat cgcctgacca ttgtgtttct
     2161 gtgcgtagcc ctatgtttag caggcttata tgctgcgcta caaatgccgt catccatctt
     2221 tccgcaaacc aatttcccgc gggttgtcat cctgctggat aatggcgtga tgccaagtaa
     2281 tgaaatgatg gcgacaatca cacgcccggt ggaagagtcc atgaaggaca tcccgggtgt
     2341 ggagaatgtg cgttcaaaaa ctgggcgcgg cactgctgag gtgaatgtct tttttaactg
     2401 gcataccgat atgacacagg cggagttgta tgtgcgtggc aaactggcga tggtgcaaaa
     2461 aacattgcca ccgaccgcaa actactctgt gtcgcggtta accttttcag catttccggt
     2521 catgggcttg agtttaacca gtgacaaaca tgatgtgacc cagctttggg aaactgcacg
     2581 ctatacgata caacccaaaa tactgcgtct caaaaatgtg gctaaagtgg gcatcgtcgg
     2641 ggggagagtg ccggaatatc atatcgtggt cgatccactt aagctgcaag cattgcatct
     2701 tggtatttct caggtagccg accttgtcgc gcgcagcaac gtcatcgccc cggctggcat
     2761 gctggaagag gactataaac tttatctgac ggtcaccgat ggccgtatac atggtacaga
     2821 agaattagag aatctcgtta ttgctgcgcc taatccggca tcggcgccag gcagcctcgg
     2881 cggcaatcca gctgccacag gcatcgccga ccatcctgtc cgcattaagg attttgcccg
     2941 tgtggagcgc aataaagagc ctgctttcta cgaggtgacc gccgacggca agaatgcagt
     3001 gttgatgaat gtctatagcc agccggatgg cagcacgctg gatattgcac aagcagtcaa
     3061 agctgaactg atctcgatta ataaaagttt gccacccggc atgaaggtac agtttttcta
     3121 tgaccagtct gagctggtag cagactccat gcaaagtgta tgggaagcga tactggttgg
     3181 gctagtattg tctatggcga ttttgtattt gtttctgcaa aactggggga cgactttggt
     3241 tgcctccatc gtcattcctg tcacggtttg ttttgccatg ctgggcctga aagtcgccgg
     3301 cttgggattt aacttaatga ctttgggcgg gattgctgca gccatcggcc tggtcattga
     3361 tgacgcgatt gtggtggtag agattattca tgcaaaaatg cagcatggcc agcaacgact
     3421 cgctgccatc actgaaggac tctctgaggt cttccggccc ttgctggctt caacgtttac
     3481 cccagtcgtg gtctttttgc cgctggcctt tctggatggt gtgcctggca ctttcttcaa
     3541 agcactggct ttaaccatgg tggtctcatt gctgtcatca ttgttgctag ccgtgaccct
     3601 gattccggcc atggccagtt ggttactgag cgctgaacag ttaacgccag ccagccacca
     3661 agctgctcag cacgcagatg agcagggcct cttcgcaaag atacaactgt tttacacgtc
     3721 acatctgaat cgtgcattgg ctaagcctaa atcactgatc tttttttcag ccttgctgct
     3781 gtcgttgagc gcctatttgt atacgctcct ggaaaacgaa tttctgccgt ttatggacga
     3841 gggcggcttt gtgttggatt accatgctcc ctggggaacc agtctgaccg agacaaatcg
     3901 tcagttgctg caggctgaac aactcatcaa gtccatgcct gaattagagg gatattccag
     3961 acgcactggc gtacgcttgg cgctggggat ttcccaggcc cacaatggtg atttcctggt
     4021 caagctgaag aaaaaccgcc agcgctcaac ggatgaagtg attactgatt tgagacagaa
     4081 attaaacacg gcagtccccg gcttgcattg ggagtttgcc ggtatcttgg gcgacctgat
     4141 tggggacctg acctggtcgc cgaaaccgct agaactaaaa ctgttttcaa ccgatatcaa
     4201 gtggttacaa aaaacagcgc ccctggtgca ggccaagtta gatcagattc caggcgtggt
     4261 ggacagcttt gacgggttgg aaagtaccgg tccttcaata catgtcaggg tcagatccgc
     4321 tgatgcggcg cgctttgggc tcactagcaa tgacatacag cttgctgtga gtacggcgat
     4381 gctgggccag aaagcttcct atgtgcttga aggcgatcgc gtggttaata tccgggtgcg
     4441 gatgcccagc gaagtgaccg ggcaaatgga taagcttatg caattaccgc tcaggactgc
     4501 tgacggccgc acggtacaac tgaagcaggt ggccgacctg gagctggtcg cgggcgagtt
     4561 ggaaatgcaa cgcgaagaca tgcgccagct ggttgccgtt acggccagac ttgagcatcg
     4621 tgaccttggt agtgcgatta atgaaattca gcataaggtg gcacaggatc ccgtgctagc
     4681 aaaagccaat attgagtttg gagggcttta ccagcagcag catgagtcat ttatcaaatt
     4741 aacgttggtc ttgttggcct cattatttct ggtattcacc gttttgctca ttcaattccg
     4801 aacctttcaa gcgcccatct ccatcgtttc aggtgctatc ctggccatgt ctggcaccgt
     4861 gattgcctgg tatgtgaccg ggacgtcggt aaacatagtc tctttattgg gcgctattat
     4921 tggggtcggt gtggtggcca aaaacggcat attgatgctg gatggcgtgc aaaagtttca
     4981 tcaggccgga ttagatttac ctgcctcatt actggcctcc ggtgtcagcc ggttgcgtcc
     5041 agtgttgatg acttcgctcg ccgcagcatt agggctgtta ccattggcat atggagtggg
     5101 ggccggatct gacatgctga agcccatggc gattgccgtg attggggcat tggtgctgtc
     5161 agtattactg tcactgattg cgacgccggt attttattat ttattgcagc gctcttcact
     5221 aaagcaaaaa acatattatc ctgagactga gatctaaagc gggcctgtca tgcacatcct
     5281 cattgttgaa gacgagaaaa agacggcagc ctttttgcaa aaagggctga ctgagcatgg
     5341 ctacaccgtg gatgtatgcc atgacggtga ggctgggctg atcctcgcca atagcgtcgc
     5401 ttttgatttg atgatctttg acatcatgct gcccttaatg gatggcttga ccttgctgaa
     5461 aaaaattcga gccaataatc aacacacgcc cgtgctgttt ttgagtgcca gggacacgct
     5521 agaagaccgg gtgaaagggc tggaattggg cgcggatgat tatctggtca aaccttttgc
     5581 ttttgttgaa gtgctggcca gggtgcgttc attattaagg cgtggtgtgg ttttgcagcc
     5641 tgaacaattg cgcatcgccg atttgacact agatcgtggt aaacataaag cgtatagagc
     5701 cgggcaattg ctggatttaa cccccaaaga atttgcattg ctggccctgt tcatgcaacg
     5761 tcctggcgag gtgttgtcaa gaacactgat tgccgagcaa gtctgggaca ttaattttga
     5821 ctttgacagc aatattattg atgttgccat ccgtagatta cgtcaaaaaa tggacgatgc
     5881 ttacagccct aagctgctgc atacagtgag aggcatgggc tatgtctttg aatctcgcta
     5941 acctgcacac agggcgctgg tctatcacgg ccaggctcac cttattattt tcagtggctg
     6001 cagcgctgat tcaggcactc ttgtctgttt atttatattc atcgttacaa cacacatttc
     6061 tgaagagtaa tcgccagttt ttgttgcgtg aaatgcaagt gctcaacaaa atcgtgcagg
     6121 cccaaccatc ggatgtggcg caccttgccg tagagcagcg agaaggattg gatttgccca
     6181 acgggaaata ttactctcgc gtgctggatg cgcagcagcg catcatcagt gaaacacccg
     6241 gtatgccaga catacttggt gccggtaacg ccatagagca cgcgcaaggc ccgagtgaac
     6301 aatggataca gcgggcaggg caccaaagct atttggtact atctagaacc ctatcaggcc
     6361 aggcggcaac agtcaccttt cagatggcac tggataccac tagccagaca cataggctga
     6421 gtgagtatca gcgtacattg ctggtggcat ttctgtttgg cgtattgctt tcggccttag
     6481 ccgctctgtt tattgcaagg catggcttga aatctcttgc cacgatggca aaagaagtcg
     6541 gtggtatcgg tgcgttgagt ttaaccaagc acctgacagt ggctgattac ccaacagaac
     6601 ttgtccattt agtgctttca tttaaccaca tgctcatgcg gcttaatcag tcttttgagc
     6661 agctacagca attctccgca gatcttgcgc atgaattgag aacgccggtc aataatctga
     6721 ttggagagag tgaggtggct ttaggtcagg tgcgtagcga tgaagactat caacagttga
     6781 ttgcctccaa tttggatgaa taccaccgtt tgagtcaaat cattgaagcg ctattgtttc
     6841 tggcaagggc ggataacact gaaattaccg ccaaaaaaca gccattaaat gccgctgagc
     6901 aagtgattgc tgtctttagt tattttgagg cactggctga agagagaggc atcaaactga
     6961 gctgtacggg tgacgcggtg gtattcgcag atatgcagtt gttaaggcgt gtactcagta
     7021 atctgattca caacgcgatc aagtttgttg cagagggcgg cgaagtgtct gttctgattg
     7081 acgctactga tgcagcaacc gtcatcacca tacaagataa cggcgcgggc attgcagcca
     7141 aacatttgtc aaaaatcttt gaccgatttt atcgtgccag tgcctctcgc actgagcatg
     7201 gaacagggtt ggggctatcc atcgtgaaat ctatcatgac cttgcatggc ggcactgttg
     7261 gaatagaaag catgattgga acaggcacct cggtgacgct cacttttgta aattgattaa
     7321 ttatcgattt tggagttata tatggaccaa gacatagaat cgtcaactac tttagatagt
     7381 caattacgcc gggcagtcat gattgttgct ttggctaacc ttgcttattt tctcgtggag
     7441 ttttttgttg caagaagtat tcattcagtt tcattgtttg cgga
//
LOCUS       DATMSK010000276        12574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_46903,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000276 DATMSK010000000
VERSION     DATMSK010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46903"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VNZ84_09250"
     CDS             <1..1142
                     /locus_tag="VNZ84_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HWU67186.1"
                     /translation="LFADNAFTLLFNGQSTFSKMKELLGSAEKNIHLEYFTFEDVDLG
                     GVTLKALLLEKLAKGVEVHVIYDAYGSQQTPPEFFESLKKAGAKITVFHPLNEGSLMQ
                     RNQRDHRKIMVVDGKVAIIGGVNLSKTYQSKGSFKTYKKETLNPLTDVVWRDTDVMVQ
                     GPILSDIQRIFLSNWDEKQPLDESNFFPKLSKQGNQFARVIATPATKQKDTDSPYYLT
                     LLSAIDSAEDRIMINAAYFVPTKAQLQALINAANRGVRVQLLVPSFTDSTLSLLVQRS
                     RYSLLLENQVEIYEMQGQVLHAKTITIDGVWSSIGSSNFDYRSAGINAEVDMVILGKE
                     VAKALEDKFKEDTTSAKKIELEEWKKRGLLERIKQLSARMFEGFL"
     gene            complement(1196..1534)
                     /locus_tag="VNZ84_09255"
     CDS             complement(1196..1534)
                     /locus_tag="VNZ84_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HWU67187.1"
                     /translation="MSTLPACPQCQSEYTYEDGDNYICPECAHEWSKVAATDSEEVKV
                     VRDANGNVLQDGDTITVIKDLKVKGSSSVVKVGTKVKNIRLVDGDHDIDCKIDGFGAM
                     KLKSEFVKKA"
     gene            1674..2471
                     /locus_tag="VNZ84_09260"
     CDS             1674..2471
                     /locus_tag="VNZ84_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWU67188.1"
                     /translation="MWLRTECDISFNVSTPTPFILMLRPRNGLHQWVARQTYMLKPHV
                     PVEEYTDNYGNLCQRLMAPVGTFSISTSADVLTADNLDIAPGSPFEEVQHLPPHVLTY
                     LLPSRYCESDKFIDLSQEIVAHVRPGYDQVSAIEQWIRENIRFNPDSPHFQLSAVEVN
                     QQREGVCRELAHLGIALCRGLCIPARMVVGYLYGLQPMDFHAWFEAYVNGRWYTFDPS
                     QSQPMGGRITVAYGRDATDVAIFNQFGPALYPEQMQVRVTQLAAAPV"
     gene            2490..3350
                     /locus_tag="VNZ84_09265"
     CDS             2490..3350
                     /locus_tag="VNZ84_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWU67189.1"
                     /translation="MKRIRIQHLTEYLFSSTVTLQQHYLLLRPREGHDLRIESSILNI
                     TPAYQIKWYRDVFDNSLAAVTFLQPSNKLTIASEVVIQHYEEAPLDFCIEPYAVNYPF
                     AYAQADWEDLAAFQQPIFANDQHAVNQWLAQLGLKHVNINTFELLTRLNQAIYTQFRY
                     QIREEPGVQTPATTLTKLSGSCRDYATLFIEACRQLGLASRFVSGYLHAPATEAGNAT
                     THAWAEVYLPGTGWKGFDPTAGKVTGTQHIAVAVSRNPETIPPVAGSFIGPNTQPKLV
                     VNVQVNLLTG"
     gene            complement(3554..5539)
                     /gene="tkt"
                     /locus_tag="VNZ84_09270"
     CDS             complement(3554..5539)
                     /gene="tkt"
                     /locus_tag="VNZ84_09270"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWU67190.1"
                     /translation="MATRVDLCNAIRALSMDAVQKANSGHPGAPMGMAEIAEVLWNHN
                     LSHNPTNPQWANRDRFVLSNGHGSMLIYSLLHLTGYDVTMDDIKSFRQLHSRCAGHPE
                     YGYAPGVETTTGPLGQGIANGVGFAMAEKLLASQFNKPGHDIVDHYTYVFLGDGCMME
                     GVSHEACALAGTWGLGKLVAFWDDNGISIDGHIEGWYTDDTAGRFKAYGWHVVSVDGH
                     DQAAIQKAIDEAKSVTDKPSLICCKTIIGKGSPNKCGSHDCHGSALGEAEVAATREAI
                     GWPHAPFEIPADVYEGWNQKDKGAKREADWNAKFDAYAKAYPAEAAEFKRRMAGELPA
                     NWKALTDAIIAETNEKAEKLATRQASQKAITALAPILPEFLGGSADLTGSNLTASKDF
                     KHVSGKEPGNYISYGVREFGMAAIMNGMALHGGLLPYGGTFHMFSDYMKNGMRMSALM
                     HQRVIYVLTHDSIGQGEDGPTHQPVENTSGLRMIPRMDVWRPADSTETTVAWVAAVER
                     TEGPTSLVLSRQAVPGIKHDAKDFDLIRKGGYVFSDVAGKADVIIIANGSELDLAIQA
                     AAELNAAGTKVRVVSMPSTNVFDRQDQAYKDSVLTPGVKRVAVEAAHPDFWRKYVGIE
                     GAVVGIDTFGESAPGGALFKHFGFTVENVVNTVKSVL"
     gene            5810..6361
                     /gene="fae"
                     /locus_tag="VNZ84_09275"
     CDS             5810..6361
                     /gene="fae"
                     /locus_tag="VNZ84_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HWU67191.1"
                     /translation="MSDDRIIMRVGEALVAGGPPGTAAEPEVAIGEMNGPMGTAFANL
                     LGDQVKGHTRVLAIMNTDIMVRPATLMVSKVTVKDPRYTNILMGTVQGAIANGVLDAV
                     RSGDIPKEKANDLGIIVSVWLSPAILEQEKIDHKALFDIHREATFKAIQKALRNEPSI
                     DWLLENQEKIVHKYYQMGLDGKI"
     gene            complement(6451..7239)
                     /locus_tag="VNZ84_09280"
     CDS             complement(6451..7239)
                     /locus_tag="VNZ84_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU67192.1"
                     /translation="MTNTTNRTYQRLAGKVAIVTGASSGIGRTTAKLFSQHGAKVVVA
                     ARRESELATLVAEIVADGGEAIAFAGDVQSEAFNQSLVELAVNSFGKLDIAFNNAGTL
                     GEIGASDQVSLQGWNDTLSINLTSAFLGAKYQIPEMAKQGGGSVIFTSTFVGYSAAFP
                     EFAAYAASKAGLIGLTQALAVEYGTKGVRVNAVLPGAVDTDMFREKNNTAESQAFLAG
                     MHALKRVGKPEEIAQSVLYLASDDAAFVSGTAHLVDGGLSVNRT"
     gene            7372..8295
                     /locus_tag="VNZ84_09285"
     CDS             7372..8295
                     /locus_tag="VNZ84_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWU67193.1"
                     /translation="METLSSIESFVRSAELGSFSAAARRLSLTPGGVSKNVAKLEADL
                     GVRLLHRSTRSLTLTEAGEQFLAQVSGGLDAIQSAIANTSTVAGQPAGTLKVGMAMAF
                     GRDHILPLLGEFLERYPAIKPDWRFDNRQVDLIGEGFDAAIGGGIDIAPGVVVRELAK
                     VHLILVASPAYLKRTFTPKTPADLTQLEALIRYSQTTNRTTPWHLKTLAGDQVTIELS
                     PRATLNDPEMLCNAAMAGLGIAHVPMPHALRHLQSGALIRVLPEWYTEAGVISLYFAA
                     KKLMPAKTRVFVDFIVDKFRVERYAEKWSAI"
     gene            complement(8343..9356)
                     /locus_tag="VNZ84_09290"
     CDS             complement(8343..9356)
                     /locus_tag="VNZ84_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU67194.1"
                     /translation="MQKQLKWGILGAARVNERLLPAIVEAKNAQLVAIASRRAGAAKA
                     TLDKYAPDAGGVACYDDMDSLLADSNVEAVYCPMANEEHAEWALKAIAAGKHVLIEKP
                     MTTRLSDIDTIESAAKTKGVTVMEGFMYRFHPQHAKVKELIDSGLIGDVLSCRASFSF
                     LMAPSRMYRINRDMAHGGGAMWDIGPYAIHALRWCFGGGDKPAEPQHVVAYGKLNEHG
                     ADVVASGVLDFGPDAQGRAQFGNFDVSFERGRKAEYELIGDKGWIKCHNMWAYPGDDP
                     VISWEAGGKSETIVLPKANHFNLEIEHFSDCVLYGKPPLLTFDDARGNCKAIEAVIAA
                     LKK"
     gene            complement(9437..10636)
                     /locus_tag="VNZ84_09295"
     CDS             complement(9437..10636)
                     /locus_tag="VNZ84_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HWU67195.1"
                     /translation="MLSSNARPYIQASVPVLQAHGLAITTAFYHNMFQAHPELKHLFN
                     MGNQANGSQQQSLAAAVFAYAANIDQAHVLQPVIDRIVHKHAAVGIKPAHYPIVGRHL
                     LGAIAEVLGEAATPPLIAAWDEAYWLLAGEFIAAEARLYQSTGYAADQWIAARITEVT
                     PAADGILSIQLAAEDQALPAFRAGQYISVAVTLPDGLRQIRQYSLSDAEQNDVYRISV
                     KREAGDTQTPQGAVSNWLHQQAVVGGQLLISHPYGEFAPDLKAKQPLVLISAGVGITP
                     MLSMLKTLVRDQSTRDVWFLHAGRSLQYVPHLDELIASTEQLPALNLALYVEDKPGFA
                     SERLVREGRMRLSEWPFNAALNAQYYLCGPLPFMIEQWQALLALGIPPQHIHREVFGP
                     ESLNQAG"
     gene            complement(10728..11168)
                     /locus_tag="VNZ84_09300"
     CDS             complement(10728..11168)
                     /locus_tag="VNZ84_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWU67196.1"
                     /translation="MQLNKFTDIGLRVLMYLSQSRFGQQVTIDEIATTFDLPRNHLIK
                     VITRLNKLGWIKATRGRNGGLELNPDSLNLRIGDILRELENTTELINCEKPVCQFHGN
                     CLLKDALQTGLESFYTHMNQHRLLDIVNNNTREKIIFMHKTHAA"
     gene            11250..12464
                     /locus_tag="VNZ84_09305"
     CDS             11250..12464
                     /locus_tag="VNZ84_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HWU67197.1"
                     /translation="MQPLIQISPPGRKRLPQGFALWNLGFRPFFLLAAVFSALSIGIW
                     VWKYATFSTLALSTVSASQWHAHEMLYGFAFAVIAGFLLTAVKNWTGIQTLHGRPLMG
                     LVACWAIARSVMLVAPGQVLLAAICDLLFNAWLIAAVALPIIKVKQWRQLGVLSKIVF
                     LTAGNAAFYSQALGWSVTGAHAALWIGLLLNVSLIMVISRRILPMFIERGVKEEVVLK
                     NNAWIDRLLMVCLAALLINILTFNAEILNTVLGLTICVSAGFRLWRWHTTGIWQVPLL
                     WSFYCGLWLVNLGFLLYAASFFAHSISVILAIHCWAIGGIGVITLAMMARVSLGHTGR
                     NIHTPPRSVIWIFGLVIFAALSRVFLPIMLPEFYRFSVMLAAGAWIVAFGLFCIGYWP
                     ILSKPRIDGTPG"
     gene            complement(12468..>12574)
                     /locus_tag="VNZ84_09310"
     CDS             complement(12468..>12574)
                     /locus_tag="VNZ84_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWU67198.1"
                     /translation="STRELFKAMKQVAETGQGPKEQIASIGCSIKWKE"
BASE COUNT         3099 a         3201 c         3116 g         3158 t
ORIGIN      
        1 acttgtttgc cgataacgcg ttcaccttgc tgtttaatgg tcagtcgacc tttagcaaaa
       61 tgaaggagtt gcttggtagc gccgagaaaa acattcacct ggaatacttc acttttgaag
      121 atgtcgattt agggggtgtc acgcttaagg ctttgctgct tgaaaagctt gccaaagggg
      181 ttgaggtaca cgtgatttat gacgcctatg gctcccagca aacgccccct gagttttttg
      241 aatccctgaa aaaagcaggg gccaaaatca cggtgtttca tcctttaaat gaaggctcac
      301 tcatgcagag gaatcagcgc gatcaccgga aaatcatggt agtggatggc aaggtggcga
      361 tcataggtgg cgtgaacctg agcaagactt atcaaagcaa aggctcgttc aagacttata
      421 aaaaagaaac gctaaaccct ttaaccgatg tggtctggcg tgatacagac gtgatggtgc
      481 aagggccgat tttaagtgac attcaacgca tctttttatc caactgggat gaaaagcagc
      541 cactggacga atccaacttt tttccgaaac taagtaaaca gggcaaccag tttgcacgtg
      601 tgattgccac accggccacc aaacaaaaag atactgactc accgtattac ctgaccctgc
      661 tctcagcgat tgatagcgca gaagatcgga tcatgatcaa tgccgcctac tttgtaccaa
      721 ccaaggcgca gttacaggca ctaatcaacg cggctaaccg tggcgtgcgt gtgcagttgt
      781 tagtgccgag ttttacagac tcaaccttgt cgctgctggt acaacgctca agatacagtc
      841 tgctgcttga aaaccaggtt gaaatctatg agatgcaggg gcaagtgctg catgccaaaa
      901 ccatcacgat tgatggcgta tggtcgtcta tcggcagctc aaattttgat tatcgcagcg
      961 ccggaattaa cgccgaggtg gatatggtga ttttaggcaa agaggttgcc aaggcgctgg
     1021 aggataagtt taaagaggac acgacctccg caaaaaaaat cgaactggaa gaatggaaaa
     1081 agcgtgggct gctggagaga attaaacaac tctcagcgag aatgtttgaa ggctttttat
     1141 aattttcctg gcaataaaaa acacctctaa ttgaggcatt ttttattgct ggcggttagg
     1201 cctttttcac aaactctgat ttgagtttca tcgcgccaaa accatcaatc ttgcagtcga
     1261 tatcatggtc gccatcgacc aagcgaatgt ttttcacttt ggtgccgacc ttcacgacgg
     1321 atgaagagcc tttgaccttc aggtctttaa tcacagtaat cgtatcgcca tcttgcagca
     1381 cattgccgtt ggcatcacgc acgactttca cttcttctga atctgttgca gcaaccttgc
     1441 tccactcatg cgcgcattcc gggcagatgt agttatcacc atcttcgtag gtgtattcgg
     1501 attggcattg tgggcaggcg ggtaatgtag acatggggac tttcttgggt caaacaataa
     1561 aaccatatat tttacagggt tattattgaa taccctagtt attcaatatc ttttgacctg
     1621 gtatccgcaa agcagtctag aatgggttgg cttgcaccac ttaaggatta cgcatgtggt
     1681 tacgtacgga atgtgatatc agctttaatg tcagcacccc cacgccgttt attttgatgc
     1741 tacgtccgcg taacggcttg catcaatggg tagcacggca gacctacatg cttaagcccc
     1801 atgtaccagt ggaggaatat accgataatt atggcaatct ttgccagcgc ttgatggcgc
     1861 ctgtgggtac attttctatc agcacatcgg cggatgtttt gactgctgac aacctcgata
     1921 ttgcgccagg gagtccattt gaggaagtgc aacatctgcc accccatgtg ctgacttatt
     1981 tgctgccctc acgctattgt gagtcagaca aatttattga tctcagccag gaaattgttg
     2041 cgcatgtgcg gcccggatat gatcaggtga gcgccataga acaatggata cgcgagaata
     2101 ttcggtttaa tccggattca ccacattttc aactttctgc cgttgaggta aaccagcagc
     2161 gtgaaggcgt gtgtcgcgag ctggctcatt tgggtatcgc cttgtgtaga ggcctatgca
     2221 ttcccgcccg catggtagtc ggttatttgt acgggctgca accaatggat tttcatgcct
     2281 ggtttgaggc ttatgtgaat ggaagatggt atacctttga tcccagtcag tcccagccta
     2341 tgggtggccg cattactgtc gcgtatggcc gtgatgccac tgacgttgcc atttttaacc
     2401 aatttggacc ggcgctgtac ccggagcaaa tgcaggttcg cgtcactcaa ttggctgccg
     2461 cacctgttta acaggtcaga aaataactca tgaaacgtat aagaatccaa catctcactg
     2521 aatatctctt ctcgagcaca gtcacactgc agcagcatta cttattgcta cgaccacggg
     2581 aaggacatga tttacgcatt gaatcctcca ttctcaatat cacgcctgcc tatcagatca
     2641 aatggtatcg agatgtcttt gataactcac tggctgctgt cactttttta cagccaagta
     2701 acaagctgac gattgccagc gaagtagtga ttcaacacta tgaagaagcg ccgctggatt
     2761 tttgcattga accctatgcg gtgaattatc cgtttgccta tgcgcaagca gactgggagg
     2821 atctggcagc ttttcagcag ccaatttttg ccaatgacca acatgccgtc aatcagtggc
     2881 tggcccagct agggctgaaa cacgtcaaca tcaatacgtt tgagctactg actcgcttga
     2941 atcaagcgat ttacactcag tttcgctacc agattcgcga agagcccggc gtacaaacac
     3001 cggccaccac cctgaccaaa ctgagtggct cttgccgtga ctatgccacg ttgtttattg
     3061 aagcttgcag gcagctggga ctggccagcc gttttgtgag tggctatttg cacgccccag
     3121 ctacggaagc aggtaacgct accacacacg catgggcaga agtatatttg ccaggaacgg
     3181 gctggaaagg gtttgacccc accgcaggca aggtgaccgg cacacagcat attgccgtgg
     3241 ctgtgtcacg caacccggaa acgatcccac cagtggcagg gagctttatc gggccaaata
     3301 cgcagcctaa attggttgtg aatgtacaag tgaatttgct cacaggctaa gcaagagtaa
     3361 ccttgccttg gatgggttgt ttcacggcat ataggcgaaa aaaaaccgct tcacgaggaa
     3421 gcggtttttt atgcagcgat ctggtctaaa ttacagaaca gatttgttca aagacgtttt
     3481 ggcttcggcc gctacgctta acatcgcctt gataaacaag gcgagttagc ctacgccgaa
     3541 atgttgtgaa taattacaaa acgcttttta cggtattcac aacattttca actgtgaaac
     3601 cgaagtgttt gaacagtgcg ccgcctggtg cggattcgcc gaaggtatcg ataccaacaa
     3661 cggcaccttc aatacctaca tacttacgcc agaagtctgg gtgagcagct tcaacagcca
     3721 cgcgtttaac gcctggagtc aatacgctgt ctttgtaagc ttggtcttga cggtcgaata
     3781 cgttggtgga tggcatggaa accacacgca ctttagtacc cgcagcgttc aattcagcag
     3841 ccgcttgaat cgccagatcc aactcggaac cgttagcgat gatgatcaca tcagctttac
     3901 cagcaacgtc ggagaataca tagccaccct tgcggatcag gtcaaagtct ttagcatcgt
     3961 gcttgatgcc tggcacagcc tgacggctca ataccaagct ggttgggcct tcagtacgct
     4021 caacagcagc aacccatgca acggttgttt ctgtagagtc agctggacgc catacatcca
     4081 tacgaggaat catacgcaga ccagaagtgt tttctactgg ttggtgtgta ggaccgtctt
     4141 caccttgacc gatagagtca tgggtcagca cgtagatcac acgttgatgc atcaacgcag
     4201 acatacgcat gccgttcttc atgtagtcag agaacatgtg gaaagtaccg ccgtatggca
     4261 acaagccacc gtgcaatgcc ataccgttca tgatcgcagc cataccgaac tcacgtacac
     4321 cgtaagagat gtagttgcct ggctctttgc cgcttacgtg tttgaagtct ttagaagctg
     4381 tcaggttaga acctgtcaag tcagctgaac cgcccaggaa ttctggcaag attggcgcca
     4441 atgcagtaat ggctttttgc gaagcttgac gagttgccaa tttctcagct ttttcgttag
     4501 tttcagcaat gattgcgtca gtcagtgctt tccagttcgc tggcaattca ccggccatac
     4561 ggcgtttgaa ttctgcagct tctgctgggt aggctttagc gtaagcgtcg aacttggcgt
     4621 tccagtcagc ttcgcgtttt gcacctttgt ctttttggtt ccagccttcg tatacgtcag
     4681 caggaatttc gaatggtgcg tgtggccagc cgatggcttc acgggtagca gcaacttcag
     4741 cttcgcctaa tgcagaaccg tggcagtcgt gtgagccgca cttgttagga gaacctttac
     4801 cgatgatggt tttgcagcag atcagtgatg gtttgtcagt cactgattta gcttcgtcga
     4861 tggctttttg aatcgcagcc tggtcgtgac catctactga aacaacgtgc cagccgtaag
     4921 ctttgaagcg gcctgcggtg tcgtctgtgt accagccttc gatgtggccg tcgatagaaa
     4981 taccgttgtc atcccagaaa gcaaccagtt tgcccaggcc ccaagtacca gccagtgcac
     5041 atgcttcgtg agaaacgcct tccatcatac aaccgtcgcc caggaacacg tatgtgtagt
     5101 ggtcaacgat gtcgtggcct ggcttgttga actggctagc cagcagtttt tctgccatgg
     5161 caaaaccaac gccgtttgca ataccctggc ccaatggacc agtagtggtt tcaacgcctg
     5221 gtgcgtaacc gtactcaggg tgaccagcac agcgggagtg caattgacgg aaagacttga
     5281 tgtcgtccat ggtcacatcg taaccagtca ggtgcagcaa ggagtaaatc aacatagagc
     5341 catggccgtt ggacaatacg aaacggtcac ggttagccca ttgtgggtta gttgggttgt
     5401 ggctcagatt gtggttccac aatacttcag caatttcagc catgcccata ggtgcgcctg
     5461 ggtggccgga gtttgctttt tgtacagcgt ccatgctcag agcacggatg gcgttgcaca
     5521 agtcgacacg agttgccatt gttttctccc tagagtgaaa gtgagcggtc ttacacaaaa
     5581 agctgcaaaa ccttgattga attctttaaa aaggccggaa aacccgcctt ttacggaaag
     5641 gctagattat tcactagaac ccatttttct tcaagcgtta atcacctttt tatggtgatg
     5701 gttttgctat ggttaaccta ttaaaaaggt cgttctggcg ccagcaaggg tagctttcag
     5761 caatggtttc ccctaccatg taaaaacatt gatagcaaag gaagtaatca tgtctgacga
     5821 tagaatcatt atgcgtgtgg gtgaagcctt ggtggctggt ggcccgcccg gtacagccgc
     5881 tgaacccgaa gtcgccattg gcgaaatgaa cggcccgatg ggcacggcat ttgccaactt
     5941 gctgggcgac caggtgaaag gccacacccg cgtactggcg attatgaaca ccgacatcat
     6001 ggtacgccct gcgaccctga tggtgagcaa ggtgacggtg aaagaccctc gctacaccaa
     6061 catcttgatg ggcaccgtgc aaggcgcaat tgccaacggc gtgctggatg ccgtgcgtag
     6121 cggcgatatc ccgaaagaga aagccaacga cttgggcatt attgtttcag tctggttaag
     6181 cccggcgatt ctggaacagg agaaaatcga ccacaaagca ctgtttgata ttcaccgtga
     6241 agccacattc aaggcgatcc agaaagcctt gcgtaatgag cctagcatcg actggttgct
     6301 ggagaatcaa gaaaaaatcg tccataagta ttatcagatg ggcctggacg gcaaaatcta
     6361 atcatgtgca gtgatgcggg caaattgcta cgcggctgac taaataaaaa aggatgcttg
     6421 cgcatccttt tttgcttcgc acagcaacac ttaagtcctg ttgacagaca aaccaccgtc
     6481 taccagatgt gctgtcccag acacaaacgc cgcgtcatct gaagccagat acaacaccga
     6541 ttgtgcaatt tcttctggtt tacccacccg tttgagagca tgcatcccgg ccaggaatgc
     6601 ctgggattct gcagtattat ttttttcacg gaacatatcg gtatccacag caccgggcaa
     6661 taccgcattc acgcgtaccc ctttagtgcc atattcaacc gcgagcgcct gggttaagcc
     6721 aatcaggcct gccttgcttg cagcataggc tgcaaactct gggaaagcag cgctataccc
     6781 gacaaaagtg gatgtaaaaa tgactgatcc gcccccttgc ttagccattt ctggaatctg
     6841 gtattttgcc cctaaaaatg cgctggtcaa gttaatggac agggtatcgt tccagccttg
     6901 cagggacacc tggtcagagg caccgatttc acccagcgtg cctgcattat taaacgcaat
     6961 atccagcttg ccgaagctgt tcacagcgag ttcaaccagt gattgattaa aagcctctga
     7021 ttgcacatca ccagcaaagg caatggcttc accaccatcc gccacgattt ctgccaccag
     7081 tgttgccaat tccgattcgc ggcgggcagc gaccacgact ttggcgccat gctgggaaaa
     7141 taattttgca gtggtacggc cgatgcctga actggcacca gtcacgatag ccactttccc
     7201 tgccaggcgt tgatatgttc tgttagtcgt atttgtcatg atgttctcct taattaaatg
     7261 aaagttttgc tgcaaccacg agtgcagtat gcgcgtctga tttaattaaa aatagtcatt
     7321 aataatgaca aatactttat actaaaagta taagataaaa gggctaacag catggaaacg
     7381 ctaagcagta ttgagtcatt tgtacgcagt gccgagctag gaagtttctc ggccgcagca
     7441 cgcagattga gcctgacacc gggaggcgtg agtaaaaatg tggctaaact ggaagcagat
     7501 ttgggcgtca gattgctgca tcgcagcacg cgtagcctga ccctgactga agcaggcgaa
     7561 cagttcctgg cgcaagtttc aggcgggcta gacgccatac aatcggcgat tgccaatacc
     7621 agcacggtgg ctgggcaacc ggctggcacg ttaaaagttg gcatggcgat ggcctttggc
     7681 cgtgaccata tcctgccact tctgggagag tttctcgagc gttatccagc cattaagcct
     7741 gactggcgat ttgataacag gcaagttgat ttgattggcg aaggatttga tgcggcgatt
     7801 ggcggcggca ttgatattgc gcctggcgtg gtggtgcgcg agctggccaa agtccattta
     7861 atactggtgg catcccctgc ttatctaaag cgcaccttca cgcccaagac accggcagac
     7921 ctaacgcaac tggaggcctt aatccgctac tcgcaaacaa ccaaccgtac cacgccatgg
     7981 catctcaaaa cgctggctgg cgatcaagtc acgatagagc tatcgccacg cgccaccttg
     8041 aacgacccgg aaatgttatg taatgcagcg atggctggat tgggcattgc gcacgttccc
     8101 atgcctcatg ccttacggca tttacaaagc ggcgcgttaa tcagggtatt gccggaatgg
     8161 tatacagaag caggtgtgat ctctttgtat tttgcggcca aaaaactcat gcccgccaaa
     8221 accagggtgt ttgtagattt tattgtggat aaattccggg tagagcgtta cgcagaaaaa
     8281 tggtcagcca tttaaaaaga ctaaaacgca tggcataaaa aaggggctca tcgccccatt
     8341 ttttatttct ttaatgccgc aatcactgct tcaatcgcct tgcaattgcc ccgggcatcg
     8401 tcaaacgtca acagcggtgg tttgccataa agcacgcaat cactaaagtg ctcaatctcc
     8461 agattgaaat gattagcttt aggcaagacg atggtttcag atttgccacc cgcctcccag
     8521 ctaatgacag gatcatcgcc ggggtacgcc cacatattgt ggcatttgat ccagccctta
     8581 tcgcctatga gctcatattc ggctttgcgg ccgcgttcaa aactcacatc aaaattgcca
     8641 aactgcgcac ggccttgcgc atccggcccg aaatcaagca cgccactggc caccacatcg
     8701 gcaccgtgtt cgttaagttt gccataggca accacgtgct gcggctcggc cggtttgtcg
     8761 ccgccaccaa aacaccagcg caaagcgtgt atcgcatacg gcccgatatc ccacatggct
     8821 ccgcctccat gcgccatatc acggttgata cggtacatgc gcgacggcgc catcaggaat
     8881 gaaaaactgg cgcgacaaga aagcacatca ccaatgaggc cactgtcgat cagttcctta
     8941 accttggcat gttgcggatg aaagcggtac ataaagcctt ccatcaccgt aacgcctttg
     9001 gttttggcag ctgattcaat ggtatcgata tcagacaggc gcgtggtcat gggcttttca
     9061 atcagtacat gcttgcccgc cgcaatcgcc ttgagcgccc actcggcatg ctcttcgttg
     9121 gccatcgggc aatacaccgc ttcgacattg ctatctgcca gcaggctatc catgtcgtca
     9181 taacaagcca cgccaccagc gtcaggcgca tatttatcca gcgtcgcctt ggcagccccg
     9241 gcacggcggc tggcgatggc gaccagctgc gcattcttgg cctctacgat cgcaggcaat
     9301 aatcgttcat tgacgcgcgc agccccgaga ataccccact taagttgttt ttgcatgcca
     9361 gcttcctttt ctgcgcggtt ctaaagagtg ccattctaac gccagtttgc aaatgatgct
     9421 tcggttacgc acgtgactag ccagcctgat tcaagctttc cgggccaaat acttcacggt
     9481 gaatgtgctg tggcgggatg cctagcgcca gcaaagcctg ccattgttcg atcataaatg
     9541 gtaatgggcc gcagaggtaa tattgtgcgt ttagcgcagc gttgaatggc cattctgata
     9601 atcgcatgcg cccctcacgc accagacgct cggacgcaaa accgggttta tcttcaacat
     9661 acaaggccaa attcagtgct ggcagttgct cggtgctggc aatcagctca tccagatgtg
     9721 gtacatactg caaactgcgg ccagcatgta aaaaccagac atctcgtgtg gattgatcac
     9781 gcaccaacgt cttcaacatc gacaacatgg gcgtgatacc aacaccggcc gaaatcagca
     9841 ccaacggctg ctttgccttg aggtcgggcg cgaattcacc ataaggatgg ctgatcagta
     9901 attgtcctcc caccactgcc tgctgatgta gccagttaga caccgcaccc tgtggcgttt
     9961 gtgtatcgcc tgcttcacgt ttgacactga tgcggtagac atcgttttgt tcggcatccg
    10021 acaagctgta ttggcggatc tgacgcaaac catcaggcaa ggtgacagcg acactgatat
    10081 attgcccggc cctgaacgca ggcaatgcct gatcttcggc cgctaactga atcgacagaa
    10141 taccgtctgc ggctggggtg acttcagtga tgcgcgcagc tatccattga tctgcggcat
    10201 agcctgtgct ttgatataac ctggcttcgg cggcaataaa ctccccggcc aacaaccagt
    10261 aagcctcatc ccaggccgca atcagcggtg gtgtggctgc ctcgcccaac acctcggcta
    10321 ttgcccccaa caaatgacgc ccgacgatgg ggtaatgtgc tggcttgatt cctaccgcag
    10381 catgtttgtg cacaatacgg tcgatcactg gctgcagtac atgggcctgg tcaatattgg
    10441 ccgcataagc aaataccgcc gcggccagcg attgctgctg gctaccatta gcctggttac
    10501 ccatattgaa cagatgcttc aactcaggat gtgcctgaaa catattgtgg taaaacgcgg
    10561 tggtaatcgc caaaccatgg gcttgcaaca cgggcacact ggcctggata tacggacggg
    10621 cattgctgga taacataaac actccttaaa ctagcatttt agatgaaaca tttaatgtaa
    10681 aaaaatggtc tttttcaaaa aagaccgatc actcaaatca ctgcaaccta tgccgcatgg
    10741 gttttgtgca taaaaataat cttctcccgg gtattgttat tgacgatatc cagcaggcga
    10801 tgttgattca tgtgggtata aaaagactcc aaccccgtct gcaaggcatc tttgagcagg
    10861 caattgccat gaaactggca caccggcttc tcgcaattaa tcagttcggt ggtattttcc
    10921 agctcgcgca aaatatcgcc tatgcgtaag ttgagcgaat ccggattcaa ctccagcccg
    10981 ccattgcggc cacgtgtcgc cttgatccaa cccagtttat tcaggcgcgt aatcacttta
    11041 atcaggtggt tacgcggcaa atcaaacgtg gtggcgatct cgtctatggt cacttgctgg
    11101 ccaaagcgcg actgcgacaa atacatcagt acgcgcaggc caatatcggt aaacttgttg
    11161 agttgcatgc ctgcattcta aatatgtttc atttacaatg caactttaaa ttgatatcct
    11221 gacaaacatt gcccgcccaa ggaacataca tgcaaccgct gatccaaatc tccccacctg
    11281 gccgcaagcg cctcccgcaa gggtttgcgc tctggaacct cggatttcgg ccatttttcc
    11341 tgctggccgc ggtattttcg gcgctatcga ttggcatctg ggtatggaaa tatgcaacgt
    11401 tttccacgct tgctttaagc accgtgagcg catcgcagtg gcatgcacat gaaatgctgt
    11461 atggctttgc ctttgccgtg attgccggct ttttgctgac ggcggtcaaa aactggaccg
    11521 gcatacaaac cctgcatggt cgaccgctca tggggcttgt cgcctgctgg gcgattgccc
    11581 gtagtgtgat gttggtcgcc ccaggccagg tgctcttggc agcaatctgc gatttactct
    11641 ttaacgcatg gcttatcgcc gcagtcgcct tgccaattat caaggtcaaa caatggcggc
    11701 agctgggagt gctgtcgaaa atcgtctttc ttacggcagg caatgccgct ttttatagcc
    11761 aggcgctagg ctggtcagtg actggcgcac acgccgcatt atggataggc ctgctgctga
    11821 atgtgagcct gattatggtg atcagccgcc ggatcttgcc catgtttatc gagcgtggtg
    11881 tcaaagaaga agtagtcctg aaaaataatg cctggataga tcgattattg atggtgtgcc
    11941 tggcagcctt actcatcaac atcctgacct ttaatgccga aatacttaac acggtgttag
    12001 ggcttactat ctgtgttagc gccggattta gactatggcg ttggcatacg actggcatct
    12061 ggcaagtgcc gttgctatgg agtttttatt gcggattgtg gttagttaac ctgggatttc
    12121 tactctatgc tgccagcttt ttcgcacata gcatttccgt gattcttgcc atccattgct
    12181 gggcgattgg tggcataggc gtgattacgc tggcgatgat ggcgcgcgtg tcgctaggtc
    12241 acaccggccg caatatccac accccaccac gcagtgtgat ctggatattt ggcctggtca
    12301 ttttcgccgc cctgagccgg gtgttcttac caatcatgct gcccgagttt taccgctttt
    12361 cagtcatgct ggccgccggc gcctggatag tcgcgttcgg gctattttgc attggctact
    12421 ggccgatact cagcaaacca cgaatagacg gcacgccagg ttaagcgtta ctctttccac
    12481 ttaatggaac aaccgatact ggcaatttgt tcctttggcc cttgacctgt ttcagccact
    12541 tgtttcatgg ccttaaataa ttcgcgtgta cttt
//
LOCUS       DATMSK010000277         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_445509,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000277 DATMSK010000000
VERSION     DATMSK010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445509"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..923)
                     /gene="murI"
                     /locus_tag="VNZ84_09315"
     CDS             complement(141..923)
                     /gene="murI"
                     /locus_tag="VNZ84_09315"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWU67199.1"
                     /translation="MCSAIAVNAPVGVMDSGVGGLSVLKHLTRQLPHEHFTYFADSAH
                     APYGNKSATEIQERCFTVADKLIGQGAKALVVACNTATAAAIANMRERYHLPIIGMEP
                     AVKPAAAASKNGIIGVLATTGTLKSAQFAALLEHYGQGVQVVTQACVGLVECIEQGQL
                     DTSQTLTLLQRYCQPLMAAGADTIVLGCTHYPFVKKHIQAIVGPEVTLIDTGAAVAKR
                     LHQVLEQKQMLNTATEAGDFVFLTSGTEETKRVMKQLFSLNS"
     gene            986..1537
                     /locus_tag="VNZ84_09320"
     CDS             986..1537
                     /locus_tag="VNZ84_09320"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWU67200.1"
                     /translation="MANRLSKIYTRTGDSGMTGLGDGSRVAKSDLRVEAMGDVDELNA
                     VLGMLLVEELDDEVRDSLVAIQHDLFDLGGELCMPGHHFIQAGRVTFLEMTLDAWNEE
                     LTPLKEFILPGGSRAASVCHLARTVCRRAERVLHALHAQSPVTPEALQYLNRLSDLLF
                     VLCRTLNRHDNVPDVLWNNQHKL"
     gene            1571..2917
                     /gene="pcnB"
                     /locus_tag="VNZ84_09325"
     CDS             1571..2917
                     /gene="pcnB"
                     /locus_tag="VNZ84_09325"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HWU67201.1"
                     /translation="MIKQLLQKVFRRRASANTHTQLPAAQIIPVGKHKINQKHISQAA
                     LKTCDQLQKAGFKAYIVGGAVRDLLLNHPPKDFDVATNATPEQVHKIFRRSRIIGRRF
                     RLVHVLWGHETIEVSTFRGHHQSEGDAETNDSGRIIRDNVFGSIEEDSVRRDFTANAL
                     YYDPKRQEVLDFHHGVADVRAKTLRIIGDPVKRYQEDPVRMLRAVRLSAKLGLKLEKS
                     TEAPIRKLADLLEDVPPSRLFDEMLKLFLSGHAIESINALRAQHLHHGLLPLLDVVLE
                     QPLGERFVMLALKNTDERIQSGKSSNPSFLFATLLWHEVLQAWQENQKRGEHMIPALH
                     EAMNEVIDKQAEKMAIHNRYTATMKEIWAMQPRFEQRSGKRPYALLTNPKYRAGYDFL
                     LLRCASGELPSEVGEWWTRFADAGTDERTAMLLPESGPKKRRRKPRKKKPTGGEAV"
     gene            2917..3399
                     /gene="folK"
                     /locus_tag="VNZ84_09330"
     CDS             2917..3399
                     /gene="folK"
                     /locus_tag="VNZ84_09330"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWU67202.1"
                     /translation="MAVALIALGSNLQQPEKQVSKAIKVLANTPGLRLIKASSLYATA
                     PVGYDNQPDFINAVVQVATDIPAPQLMQLLLDIEQTFGRERPFPNAPRILDLDLLDYQ
                     GMHLDTELLKLPHPRMHERGFVIVPLAEIVPDFMLPQGKTVVEWMAESQHQDIRKIGF
                     "
     gene            3457..>4019
                     /locus_tag="VNZ84_09335"
     CDS             3457..>4019
                     /locus_tag="VNZ84_09335"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HWU67203.1"
                     /translation="MFNRFPYVVIEGPIGSGKTTLARMLADKFSVELLSEKAEANPFL
                     TRFYQDAQRYALPTQLFFLFQRSRQIEDMAQRDMFGQPTVSDFFLEKDPLFARLNLDD
                     EEYSLYHQIYHHLQLKAPKPDLVVYLQTPVDALMDRIEERNISYEQDMPREYIARLAE
                     AYSEFFHAYDASPLLIVNNEKLNIIKDP"
BASE COUNT          977 a          970 c         1084 g          988 t
ORIGIN      
        1 gttactttct gtggggcaag caacagaaag tgactcgcct agaggcgaaa aagaaatatt
       61 gtaaatgcga ggatgtgggt gctaatgttt gtgacaataa aaaactgctt ttcaagacat
      121 tgcagtttga actgaaagac ctatgaatta aggctaaaca attgtttcat tacccttttt
      181 gtttcttccg tcccactggt taaaaacaca aaatccccag cttcagtagc tgtattaagc
      241 atctgctttt gttccaacac ttgatgcagt cgcttggcaa cagccgcccc ggtatcaatc
      301 aaggtgacct cagggcccac aatcgcttgg atatgttttt ttacaaacgg gtaatgtgtg
      361 cagccgagca caatggtatc tgcacccgct gccatcagtg gctggcagta tcgttgcaaa
      421 agagtcagtg tctgcgatgt gtctagttgc ccttgctcta tgcactccac caagccgacg
      481 caggcttgtg tcacgacttg cacgccttga ccgtaatgct ctagcagggc ggcgaattgc
      541 gcgcttttga gggtgccagt ggtggcaagt acaccgatga tgccattttt actggcagca
      601 gcagcgggtt taacggcggg ttccataccg atgataggca agtgataacg ctcgcgcatg
      661 ttagcgatgg ccgcggcggt ggcggtgtta caggcgacca ccagcgcttt ggcaccttgg
      721 ccaatgagct tgtcggcgac agtaaaacag cgctcctgga tttcggtggc agatttgttg
      781 ccataaggtg catgggcact gtcagcaaag taagtgaaat gctcgtgtgg cagttggcgg
      841 gttaaatgct taagcacgct gaggccgccg accccggaat ccatcacccc gaccggggcg
      901 ttgactgcaa ttgctgagca catcgaaatg gcacggtatg atttataatg ggtggattat
      961 atcggatttt atacgggtta aaggaatggc caatcggctg agcaagattt acacgcgtac
     1021 cggcgatagc ggtatgaccg ggctgggtga cggttcgcgc gtggcaaagt ctgatttgcg
     1081 tgtagaagcc atgggcgatg tcgatgagct caatgcagtg ctcggcatgc tattggtaga
     1141 agagctggat gacgaggtac gcgacagctt ggtggcgatc cagcatgact tgtttgacct
     1201 ggggggcgag ttatgtatgc ctggtcatca ttttattcag gctgggcgcg tgacgtttct
     1261 ggagatgacg ctggatgcat ggaatgaaga gctgacgccg cttaaagagt ttatcttgcc
     1321 gggcggtagc cgagcagctt cggtgtgcca tttggcgcgt acggtctgcc gccgtgccga
     1381 gcgtgtgtta cacgccttgc atgcgcagtc gcctgtcaca ccggaagcct tgcaatatct
     1441 gaaccggtta tccgatttgc tgtttgtcct ctgccgcacg ctgaaccggc atgacaatgt
     1501 gccagacgta ttatggaata accaacataa actgtagcgc ggcgattgct ggcgctcgaa
     1561 atgcaaaaga atgattaaac aattactaca aaaagtattc cgtcgccgtg ccagcgcgaa
     1621 tacgcatacc caattgcctg cggcgcaaat tattccagtg ggcaaacaca agatcaacca
     1681 gaaacatatt agccaggcgg ccctgaaaac ctgcgaccaa ttacagaagg ctggtttcaa
     1741 ggcttatatt gtcggtggtg ccgtgcgcga tctgctactc aatcatccgc ccaaggactt
     1801 tgatgtagcc accaatgcca cgccagagca ggtgcacaag attttcaggc gctctcgtat
     1861 tatcggccgc cgattcaggt tggtgcatgt gttgtgggga catgaaacca tagaagtctc
     1921 gacgtttcgt gggcatcacc agagcgaagg cgatgcggaa accaacgaca gtggccgcat
     1981 tatccgcgac aacgtgtttg gtagcattga agaggactcg gtgcgccggg atttcactgc
     2041 caatgccttg tattacgatc ccaagcggca ggaggtccta gatttccatc atggggtggc
     2101 tgatgtccgc gccaaaacct tgcgtattat tggtgatccg gtcaagcgtt accaggaaga
     2161 ccctgtgcgc atgctgcgcg cggttcgctt atctgccaaa ttgggcctga agctggaaaa
     2221 gtctaccgaa gcaccgatcc gcaaactggc tgacctgctc gaagacgtgc cgcccagccg
     2281 actttttgac gaaatgctca aactgttttt gtccggccat gccattgaga gcatcaatgc
     2341 cttgcgtgca caacacctgc atcatgggtt gctgccactg ctcgatgtgg tgctggagca
     2401 gccactcggt gagaggtttg tcatgctggc gcttaaaaat actgatgagc gcattcagtc
     2461 tggtaaatct tccaacccca gtttcttgtt tgcgaccttg ttatggcatg aagtgctaca
     2521 agcctggcaa gaaaaccaga aacgtggcga gcatatgatt cctgccttgc atgaggccat
     2581 gaacgaagtc atcgacaagc aggccgaaaa gatggcgatc cataatcgct atactgcaac
     2641 tatgaaagaa atctgggcca tgcagccgcg ttttgaacaa cgctctggta agcgccctta
     2701 tgcgttattg accaatccca aataccgcgc gggctatgat ttcttgctgc tgcgttgtgc
     2761 ctctggtgag ttgccttcag aagtaggcga gtggtggacg cgttttgctg atgctgggac
     2821 tgatgaacgt actgccatgt tgttgccaga aagtggaccc aaaaagcgtc gccgcaaacc
     2881 acgcaagaaa aagccaactg gcggggaagc ggtttaatgg cggtagccct gattgcgcta
     2941 ggcagtaatt tgcaacagcc agaaaagcag gtcagcaagg caattaaggt gttggctaac
     3001 acgccggggc taaggttaat caaggcttcc agtctgtatg cgacagcccc ggttggctat
     3061 gataatcagc ctgactttat caatgccgtg gtgcaggtgg caactgatat ccctgccccg
     3121 caattgatgc agcttttact cgatattgaa cagacgtttg gccgtgagcg cccattcccc
     3181 aatgcaccgc gtatcctgga tctggacttg ctggattatc aaggtatgca ccttgatact
     3241 gagttgttga aattgccgca ccctcgcatg catgagcgtg gttttgtgat tgttccgctg
     3301 gctgaaattg tgcctgactt tatgctgccg caaggaaaga ctgttgtaga atggatggca
     3361 gaatctcagc accaagatat tagaaagatc gggttttgag aaaactggga cgtgcgcaca
     3421 ctggcactga tttaataagc tgaacgagca agaacaatgt ttaaccgttt cccttatgtc
     3481 gtgattgaag gcccgatagg cagtggcaaa accacgctgg cacgcatgct ggcagacaag
     3541 ttttcggtcg agttgttatc cgaaaaagct gaagcaaacc cattcctgac ccgtttttat
     3601 caggatgccc agcgttacgc cttgccgact caactatttt tcctgttcca gcgttcgcgc
     3661 cagattgagg atatggccca gcgtgatatg ttcggtcagc ccaccgtttc ggacttcttc
     3721 cttgagaagg atccgctgtt tgcgcggctg aacctggatg atgaagaata ttctctctat
     3781 caccagattt accatcacct gcagctaaaa gcacctaaac cagatttggt ggtgtattta
     3841 caaaccccgg tcgatgcgct gatggaccgg attgaagagc gcaatatcag ctacgagcaa
     3901 gacatgccac gcgaatacat tgcacgtttg gccgaagctt acagcgaatt ttttcatgcc
     3961 tatgatgctt cgccgttatt aatcgtaaat aatgaaaagc tcaatatcat taaagatcc
//
LOCUS       DATMSK010000278         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_22567,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000278 DATMSK010000000
VERSION     DATMSK010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22567"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VNZ84_09340"
     CDS             complement(<1..471)
                     /locus_tag="VNZ84_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HWU67204.1"
                     /translation="MSKALVWLRRDLRDDDHAALYHALKRHTQVIVAFVFDTDILEHL
                     TDKHDRRVEFIWESVRELKQSLQSHGSDLIVLHGSAQVEIPALAHKLQVTAVYANKDY
                     EPVAIARDEVVANTLLQANRTLHLFKDQVLFEQDEVMTQTGKPYGVFTPYKNGHL"
     gene            complement(474..941)
                     /gene="rlmH"
                     /locus_tag="VNZ84_09345"
     CDS             complement(474..941)
                     /gene="rlmH"
                     /locus_tag="VNZ84_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HWU67205.1"
                     /translation="MKLNIISVGHKMPDWVESACAEYLKRMPRELETRIIEIKPDKRA
                     AGKNSEVVQEAEAKRILEVAGKDYLVALDERGQAVTTLQLAERLKSWQEMGRDVSLVI
                     GGADGLHAQLKAQAQWLFSLSKLTMPHGMVRVMLAEQLYRAHTVLSGHPYHRE"
     gene            complement(1060..1431)
                     /gene="rsfS"
                     /locus_tag="VNZ84_09350"
     CDS             complement(1060..1431)
                     /gene="rsfS"
                     /locus_tag="VNZ84_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWU67206.1"
                     /translation="MQPNIDVLSLEDMKLAVVDALEDIKAFDITVMDVRHMTAMTSYM
                     IVASGNSTRQCKAIADNVREKLKEKGVDTRGVEGEKEGEWVLVDLGDIVVHVMVPATR
                     AYYNIEQLWSESQSRRAASAA"
     gene            complement(1446..1826)
                     /gene="panD"
                     /locus_tag="VNZ84_09355"
     CDS             complement(1446..1826)
                     /gene="panD"
                     /locus_tag="VNZ84_09355"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HWU67207.1"
                     /translation="MQRTMLKSKLHRVRVTHSELHYEGSCAIDEALLEAANIHEYEQI
                     QIYNINNGERFTTYAIRAERHSGVISVNGAAAHKADPDDLIIIASYSQYTEAELSNYH
                     PQLVYVDAQNRIVEQKNHIPAQAA"
     gene            complement(1932..2786)
                     /gene="panC"
                     /locus_tag="VNZ84_09360"
     CDS             complement(1932..2786)
                     /gene="panC"
                     /locus_tag="VNZ84_09360"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWU67208.1"
                     /translation="MNVFHTKREMRAFLDSQCGKIVGFVPTMGNLHAGHCGLVTQARQ
                     HADIVVVSIFVNPLQFGVNEDLGSYPRTLQADCEKLQSVGADVVFAPAVEEMYPDFDG
                     QDLHQQMVIQPPPLADDLCGASRPGHFAGVATVVAKLFHMVRPHVAVFGKKDYQQLAV
                     IRALVRQLNFDIEILAGETMRESSGLAMSSRNGYLTPPEKEQAAQLYQQLLGIKSALL
                     AGRREYALLCEQASFALAQQGWQVDYVEIRRQHTLTLPDAQTRDWVVLAAARLGKTRL
                     IDNCEVSS"
BASE COUNT          816 a          852 c          665 g          778 t
ORIGIN      
        1 cagatgccca tttttatacg gcgtaaacac gccgtaaggt ttgccagttt gcgtcatgac
       61 ctcgtcttgc tcaaacaaaa cctggtcttt aaataagtgc agcgtcctgt ttgcttgaag
      121 tagagtattg gcgacgacct catctcgcgc aatcgcgacc ggttcataat ctttattggc
      181 atacactgcc gtcacttgta acttgtgtgc aagggcagga atctcaacct gcgctgaacc
      241 atggagcaca atcaaatcgc tgccatgtga ttgcaaggac tgtttgagtt ctcgaacact
      301 ctcccagata aactccaccc ggcggtcgtg cttgtctgtg agatgctcca gaatatcagt
      361 atcaaaaaca aaagcgacaa ttacttgcgt atgccgcttt aaagcatggt atagcgcggc
      421 atgatcgtca tcgcgcaggt cacgacgtaa ccagaccaag gctttggaca tatttattcc
      481 cggtgataag gatgaccgct tagaaccgtg tgcgcgcgat aaagttgctc ggccagcatg
      541 acgcgcacca tgccatgcgg catggtcaat ttagataggc tgaacagcca ttgcgcctgg
      601 gctttaagct gcgcatgcaa accatccgcg ccaccgatca ccaatgacac atcacgcccc
      661 atctcttgcc agcttttgag cctctccgct agctgcaagg ttgtcacagc ctgcccgcgc
      721 tcatccagcg caaccaggta atctttaccg gccacttcca gaatacgttt ggcttctgcc
      781 tcttgcacca cttcgctatt tttacctgca gcacgcttat ccggtttgat ttcaatgatg
      841 cgtgtctcta gctcacgggg catgcgcttt aaatactctg cacacgcaga ttcgacccaa
      901 tcaggcattt tgtggccaac agaaataata ttgagtttca ttgaggaacc aatagtgaca
      961 tcggggaaag cggttcgcta cttgccgcca ctcaacgcag aaagctttgt gcattgcgca
     1021 cgcatgcagc gtgcgaacga gaccgggaca gtgcgacgct taggcagccg acgcagcgcg
     1081 gcgagattgt gactcgctcc acagctgctc gatgttgtag tatgcgcgcg tggcaggcac
     1141 catgacgtga accacaatat cacctaggtc aaccaagacc cattcgcctt ctttctcacc
     1201 ctcgacaccg cgcgtatcta cgcctttttc cttgagcttt tcgcgtacat tatcggcaat
     1261 cgccttgcat tggcgggtag agttaccgct ggcaacaatc atataactgg tcatggccgt
     1321 catatgacgc acatccataa ctgtaatatc aaaagcttta atatcttcca gcgcgtcaac
     1381 aacggccagc ttcatgtctt caagagacaa cacatcaatg ttaggttgca tagggatact
     1441 caaaatcagg ccgcttgcgc aggaatatgg tttttctgtt caacgatacg attttgcgca
     1501 tcgacataaa ccaattgcgg atggtaattg gaaagctcag cttcggtata ttgcgaataa
     1561 ctggcaatga tgatcaggtc atcaggatct gctttatgcg ctgccgcgcc attcactgaa
     1621 atcaccccag aatgacgttc agcacgaatc gcataggttg tgaaacgctc accattgttg
     1681 atgttgtaaa tctggatctg ctcatattca tgaatattgg cagcttccag caaggcttca
     1741 tcaatcgcac agctgccctc gtagtgcagc tcgctatgcg tgacacgcac ccgatgcagc
     1801 tttgatttca acatggttct ttgcattgcg tcccttcagt caaccgcccg ctcgcgcgga
     1861 aaaggtcgta attataaagt tatcaatggc ttagcgcaaa ataaatttat aaaaaaccgc
     1921 attaaaatag attagctact gacttcacaa ttgtctatga gtcgcgtttt tcccaacctg
     1981 gctgctgcca ggacaaccca gtctcgcgtt tgggcatccg gcaaggtcag tgtatgttga
     2041 cgacgaatct ctacataatc cacctgccag ccctgttggg ccaatgcgaa actggcctgc
     2101 tcacacaaaa gtgcatactc ccgccgccca gctaacaagg cgcttttaat acctaaaagt
     2161 tgctgataca actgagctgc ctgttctttt tcaggcggcg ttaaataacc atttctggag
     2221 ctcatggcca atccggaaga ttcacgcatc gtttcacccg ccagaatttc aatatcaaaa
     2281 ttaagttgcc gcaccagtgc acggatgacc gccagctgtt gataatcttt tttgccaaag
     2341 accgctacgt gcggcctgac catgtgaaaa agcttagcca ccactgtcgc aacgcctgca
     2401 aaatgccccg gccggctcgc tccacagagg tcatctgcca atggcggcgg ctggatgacc
     2461 atctgctgat gtaaatcctg accatcaaaa tcagggtaca tctcctccac agcgggtgca
     2521 aacaccacat ccgctccaac agactgcaac ttttcgcaat cagcctgcaa agtacgtgga
     2581 taactaccca aatcctcatt gacaccaaac tgtagaggat tcacgaaaat actcaccact
     2641 acgatatcgg cgtgctgcct ggcctgtgtc accaacccac aatgccctgc atgtaagttg
     2701 cccatggttg ggacaaagcc aacaattttg ccgcactgac tatccaaaaa agcacgcatt
     2761 tcgcgtttgg tatgaaaaac attcataaaa acctactatc catccaccaa ttccgcgcac
     2821 tataacacgg cacgcaaatt cggtatcagc atgcaaaatt cgatcatgtc tgcacctctc
     2881 cctctaaacg ctctagcaat cgtcgaatat cttccaagca tttacaaact ctttaaagac
     2941 agtggattcc tacatgattt tgatctttta accgataacc agcctctgtt aaaaaagaga
     3001 tcatatttcc acgttggaca ttaaacaaac agcacaacca catcagttag gagaatcaaa
     3061 tgggtaaata ttttatcgct tggctattgg gcgttcccgg catcgtgtta c
//
LOCUS       DATMSK010000279         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_93297,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000279 DATMSK010000000
VERSION     DATMSK010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93297"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..345
                     /locus_tag="VNZ84_09365"
     CDS             142..345
                     /locus_tag="VNZ84_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWU67209.1"
                     /translation="MATGTVKWFNDSKGFGFITPDDGGDDLFAHFSAIVESGYKSLKE
                     GQRVSFDETEGPKGKQASNIQKA"
     gene            complement(428..1678)
                     /gene="icd"
                     /locus_tag="VNZ84_09370"
     CDS             complement(428..1678)
                     /gene="icd"
                     /locus_tag="VNZ84_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWU67210.1"
                     /translation="MESAMSKIKVPATGSKITVNADNTLNVPDQPIIPFIEGDGIGVD
                     ITPPMMKVVDTAVEKAYGGKRKISWMEVYAGEKSTTVYDKDTWLSDETMAVLKDYVIS
                     IKGPLTTPVGGGIRSLNVTLRQELDLYVCLRPIQYFTGVPSPVKHPEKTDMVIFRENT
                     EDIYAGIEWAAGTEEVKKVINFLSDMGVRKKIRFPEASSIGIKPVSIDGSKRLIRKAI
                     QYAIDNNRKSVTITHKGNIMKFTEGGFRDWGYQLAKEEFGAELIDGGPWCKLPNGIVI
                     KDCIADAFLQEILLHPEDYDVVAALNLNGDYMSDALAAQVGGIGIAPGANLSDTVAMF
                     EATHGTAPKIAGKNLANPSSLILSAEMMLRHMGWVEAADLVLNGVAKAIASKRVTGDF
                     SSQMEGATQVGTAEFGIEIIKQMI"
     gene            1677..2219
                     /locus_tag="VNZ84_09375"
     CDS             1677..2219
                     /locus_tag="VNZ84_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HWU67211.1"
                     /translation="MLLLFNKPFNVVCQFTAHEKHATLKDYIPIPNVYAAGRLDTDSE
                     GLLILTDDGGLQHRLSHPKHKQVKTYWAQVEGAPTEAELMPLQQGVDLGDFVTQPAQV
                     KIITEPDQLWQREPPIRERKHIPTTWLEIKISEGKNRQVRRMTAHVGFPTLRLIRYAI
                     GPYALDGILLGQYQEVSLRR"
     gene            2224..2928
                     /locus_tag="VNZ84_09380"
     CDS             2224..2928
                     /locus_tag="VNZ84_09380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HWU67212.1"
                     /translation="MKQVVVFRHACCEGAGYLGTFLARHAIPWCEVRIDKGETVPASV
                     DAYSGIVLMGGPMSVNDDLPWIPPLLALIREAVDKDVPLLGHCLGGQLISKALGGVVS
                     KNPVKEIGWGEVQVVDHPDAREWFGELQGFNSFHWHGETFTVPEGATHVLSGPYCANQ
                     AYVMGKHIGLQCHIEMTPDMVKMWTDQWKNDIASAANSPAVQSADTMLAGVAEDCSVL
                     HQVADKVYSRWIQGLA"
     gene            complement(2938..>3703)
                     /locus_tag="VNZ84_09385"
     CDS             complement(2938..>3703)
                     /locus_tag="VNZ84_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWU67213.1"
                     /translation="LANIQQSGLAADDTQARKLVKTSRTEAGKAVMETFFIWGSASDK
                     LMRLIQHVHGWSHVTEAQVQNKGLIFLTPHLGCFEITSIYYGEHAPITVLYRPPKLRW
                     LSDLVVKGREKGNVQMAPANASGVKQLLHALKAGQAIGILPDQIPRQGEGEWADFFGK
                     PAYTMGLASKLASKTDAVVIMAFGERLPSGKGFNIHLTRVDDIHTPALLNQAIARQVS
                     QCPAQYLWQYNRFKQRRYALHKLEKTQAEQTKDGQV"
BASE COUNT          854 a          883 c          989 g          977 t
ORIGIN      
        1 cacttataaa tccttaaggc gtttgggttc aggttttttg tagccgtctt taagtaaagc
       61 aaagcttgtt tattgcgact ggagtacttg agacaaactt ttggggcgcc tccctgttaa
      121 gtaaaaaagt aaggaagtaa aatggcaaca ggtaccgtaa agtggttcaa tgattctaag
      181 ggttttgggt ttattactcc ggatgatggt ggtgacgatt tgttcgcgca cttttccgca
      241 attgtagaat ccggttacaa aagcctcaaa gagggccagc gcgtgtcttt cgatgagact
      301 gaaggtccaa aaggcaagca agcaagcaat atccaaaaag cataacgatt gtttaggggc
      361 accaaatgca aaacggctcg cagatgcgag ccgttttttt attgagtctg ccgagaaagg
      421 ctaaaaatta aatcatttgc ttgatgattt caatcccaaa ctcagcagtg ccgacttgcg
      481 ttgcgccttc catctggcta ctgaagtcgc cggttacgcg tttgctggca atcgcttttg
      541 caaccccatt gagtaccagg tccgctgctt caacccagcc catatgacgc aacatcattt
      601 cggctgacag aatcagtgag cttggattgg ccagattttt accggcaatt ttaggcgccg
      661 taccgtgggt agcttcaaac atggcaacgg tatcactcag gtttgcgccc ggtgcgatgc
      721 cgatgccgcc gacttgtgcg gccagggcat cactcatgta atcgccgttg aggttgagcg
      781 cagccaccac gtcgtaatct tcagggtgca gcaaaatctc ttgcaggaag gcatcggcga
      841 tacagtcttt gatgactatg ccgtttggca gtttgcacca tgggccgcca tcaatcagct
      901 ctgcgccaaa ctcttctttg gccaactggt agccccaatc acggaagccg ccttcggtga
      961 acttcatgat gttgcccttg tgcgtgatag tgaccgattt gcggttgtta tcaatcgcat
     1021 attgaatcgc tttgcggatc aagcgtttgc tgccatcgat agagacaggc ttgatgccga
     1081 tggaagatgc ttcggggaaa cggattttct tgcgtacgcc catgtcactc aaaaaattga
     1141 tcactttctt gacttcttca gtacccgccg cccattctat gccagcataa atatcctccg
     1201 tgttctcacg gaaaatcacc atgtcggttt tctcaggatg cttgaccggg ctagggacgc
     1261 cggtgaaata ctggataggg cgtaggcaga catataagtc cagttcctgg cgcagggtca
     1321 cgttcagtga gcggatgcca ccgccgactg gcgtagtcaa tggacctttg atggaaatga
     1381 catagtcttt caagacggcc atggtttcat cagacagcca ggtatctttg tcatagacgg
     1441 tggtggattt ttcgccggca tagacttcca tccaggaaat tttgcgtttg ccgccgtagg
     1501 ctttctcgac tgcagtatcc acgaccttca tcatgggtgg cgtgatgtca acgccgatac
     1561 catcgccttc gataaacggg ataataggtt ggtcgggaac atttagggta ttatcagcat
     1621 tgacggtgat tttgctgcca gtggcaggca ccttgatttt agacatagca ctttccatgt
     1681 tattactgtt taacaaaccc tttaacgtcg tatgccagtt tactgcgcat gagaaacacg
     1741 cgacgctcaa ggactatatt ccgattccca acgtgtatgc tgccgggcgg ttagataccg
     1801 attctgaagg actgcttatt ctaaccgatg acggcggatt gcaacatcgt ttaagccatc
     1861 ccaaacacaa acaggtcaaa acctactggg cgcaagtaga aggtgctcct acagaagcag
     1921 aattgatgcc actgcaacag ggggttgatc ttggtgattt tgtcacccag cctgcacagg
     1981 tgaaaattat cactgaacct gaccaattgt ggcaacggga gccccctatt cgcgaacgaa
     2041 aacatatccc gaccacctgg ctggagatta aaatcagtga aggcaagaac cgccaggtgc
     2101 ggcggatgac cgcccacgtg ggatttccga ccttgcgcct gattcgttat gcgattggcc
     2161 cgtatgcatt ggacggaatc ttgcttggac aatatcagga agtcagttta agaaggtaat
     2221 ttcatgaaac aggtggtggt attcaggcac gcttgctgcg agggtgccgg ttatttaggc
     2281 acttttttag cgcggcatgc tattccatgg tgcgaggtgc ggattgataa aggtgagact
     2341 gtaccggcct cggtggacgc ttacagtggc attgtactga tgggtggccc catgagtgtg
     2401 aatgatgacc tgccatggat tccaccattg ctggcgttga tccgcgaagc ggtagacaaa
     2461 gatgtgccct tgcttggaca ctgcctgggc gggcaactca tcagcaaggc cctgggcggc
     2521 gtggtaagca aaaaccccgt caaggagatc ggctgggggg aggtgcaagt ggtcgatcat
     2581 ccggatgcgc gagagtggtt cggtgagctg caggggttta acagctttca ctggcatgga
     2641 gaaacattta ccgtgccgga aggtgcaacc catgtgttat ctggcccgta ttgcgctaac
     2701 caggcttatg tcatgggtaa gcatattggt ttgcagtgcc atattgaaat gaccccggat
     2761 atggtcaaga tgtggactga tcaatggaaa aacgatattg ccagcgccgc caacagtccg
     2821 gcggtacaat cggcagatac catgctggct ggcgttgctg aagattgctc cgttctgcac
     2881 caggtcgcgg ataaggtgta tagccgctgg atccaagggt tggcttaact gttttcctta
     2941 aacctggcca tctttggtct gttcggcttg ggttttttcc agtttatgca atgcatagcg
     3001 ccgttgtttg aaacggttat attgccataa gtattgtgct gggcactgtg acacctgtct
     3061 tgcaatcgcc tggttgagca gggctggcgt gtggatatca tcgacgcgtg tcaaatggat
     3121 attaaagcct ttcccggatg gcaagcgctc accaaaagcc atgatgacta ctgcgtcagt
     3181 cttgctggcc agtttgctgg ctaaacccat ggtgtacgcc ggtttgccaa aaaaatcagc
     3241 ccattcacct tcaccttgtc tggggatttg atccggtaag atgccaattg cttgaccagc
     3301 tttcagcgcg tggagtaatt gctttacacc gctggcattg gccggcgcca tctgaacgtt
     3361 gcctttctcc ctgcctttta ccaccaggtc gctcagccat cgcagttttg gcggtctgta
     3421 caagacagtg attggtgcgt gttcgccgta gtaaatcgac gtgatttcaa agcaacccag
     3481 gtgcggtgtc aggaaaatca ggcctttgtt ttgtacttgg gcttcagtca catgtgacca
     3541 gccatgtacg tgctgtatca gtcgcatgag cttgtctgaa gcgctgcccc aaatgaaaaa
     3601 tgtttccatc acggctttgc cggcttctgt ccggctggtt ttcaccaatt ttctggcttg
     3661 cgtatcgtct gcggcgaggc cgctttgctg gatattggca agg
//
LOCUS       DATMSK010000280        12095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilus sp. isolate SMAG_U12984 k141_23169,
            whole genome shotgun sequence.
ACCESSION   DATMSK010000280 DATMSK010000000
VERSION     DATMSK010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943585
            Sequence Read Archive: SRR7448260
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilus sp. (soil metagenome)
  ORGANISM  Methylophilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylophilus.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:51:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,889
            CDSs (total)                      :: 1,873
            Genes (coding)                    :: 1,867
            CDSs (with protein)               :: 1,867
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Methylophilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23169"
                     /isolate="SMAG_U12984"
                     /isolation_source="soil"
                     /db_xref="taxon:29541"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /gene="argH"
                     /locus_tag="VNZ84_09390"
     CDS             complement(<1..706)
                     /gene="argH"
                     /locus_tag="VNZ84_09390"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWU67214.1"
                     /translation="MTTQQPNTQSSLHQKDQAWSGRFNEPVAELVKRYTASVDFDKRL
                     AKFDIQGSIAHATMLGAQGIISAQDVEDIKRGLGEILAEIEAGQFEWLLDLEDVHLNI
                     ERRLTEKIGDAGKRLHTGRSRNDQVATDVRLWLRSVVDDTIAGIRALQTSLLDLAENN
                     AATIMPGFTHLQVAQPVTFGHHLMAYFEMLNRDAERFADARKRINRLPLGAAALAGTS
                     YPIKRELVAELLGFDSV"
     gene            811..1830
                     /locus_tag="VNZ84_09395"
     CDS             811..1830
                     /locus_tag="VNZ84_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWU67215.1"
                     /translation="MRKTSRIPNFRNLGIHLRVLLLHLCLLALLTVYDGQSGWNVTLW
                     LNELARIGSQWAPPLLLVMGALTLAYPVISRWPYRLALASIFLLAISLTAFLAYGYHH
                     WITPLATAQLWQLMLLCVADVLVCLYYLDLLQRAYSPAIAEARLQALQARIRPHFLFN
                     SLNAALSLIRSQPQRAETALEDMAELFRVLMADNRDLVPLSQEIALCQQYLNIEKLRL
                     DERLQYDWQLSDLPSEMMIPPLILQPLLENAVYHGIEPQSQGGMIDIEIRPSGNSLLL
                     RISNPLPPRSDKSGGNKMALKNIRERLRLHYDLEAQLRQYEADQRYIVEILIPTRPLS
                     EVVHG"
     gene            1823..2569
                     /locus_tag="VNZ84_09400"
     CDS             1823..2569
                     /locus_tag="VNZ84_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWU67216.1"
                     /translation="MAKQRLLIVDDEPLARQRLRDLVSDIGGYEVLGEAANGVQAIAL
                     LQHAQVDIVLADIRMPVMDGIELAQHLRSLEKPPHLIFTTAYDHHALQAFELNAIDYL
                     LKPIRSERLATALAKARPLQPEQSQALQTLQQTHAHLSIQERGKVLLIPLSDVLYFRA
                     ELKYVTVRTREKSYLLETSLNQLEQAYAEQFVRIHRNALVARDAISGFEKQRISEGEE
                     AGETSWVVLIRGIPDTLTVSRRHQHMIKAT"
     gene            complement(2586..3254)
                     /locus_tag="VNZ84_09405"
     CDS             complement(2586..3254)
                     /locus_tag="VNZ84_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67217.1"
                     /translation="MQYQIDIFLSSLNQFWVELVHFVPKLLAAIVILFFGWLVAKAVR
                     LGAKRILELTHFDTFAKSSGLEAFMRSANYKVTLSGIISEVLYWLVILLFVITCANSL
                     GLNEVAALLKELASYLPHIIVAILVVIFGTLFARFINRLVFAWLHSIKFEQALIISTS
                     AEYCIQILAIFIALEQLGIGMQLIHSLFVIVFGAVFLALAIAFGLGGRDWAAKVIDQA
                     QKKK"
     gene            3463..4407
                     /gene="hemC"
                     /locus_tag="VNZ84_09410"
     CDS             3463..4407
                     /gene="hemC"
                     /locus_tag="VNZ84_09410"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWU67218.1"
                     /translation="MSTFNPPAKLVIASRESALAMWQAKHIQARLQALYPQCDVSILG
                     MTTTGDQILDSPLSRIGGKGLFVKELENALADGRADLAVHSMKDVPMHLPEGFTLTAT
                     GEREDPRDAFVSNTYSSLEELPEGSIVGTSSLRRQSQIQARFPHLKIESLRGNVQTRL
                     RKLDEGQYAAIILAAAGLIRLELGDRIKGFIDPADSIPAVGQGALGIEINADRKDLVD
                     VLAPLNHVDTQLCVEAERGFSRALAGSCTVPLGAYATKQGDTITMQGFVASVDGQQML
                     KETVTGHAAEAEKLGQQLALQLVSRGADKILASLDGVK"
     gene            4404..5168
                     /locus_tag="VNZ84_09415"
     CDS             4404..5168
                     /locus_tag="VNZ84_09415"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWU67219.1"
                     /translation="MTLPLAGRTIAVTRPPEQATKLTAAIEAAGGVVISFPLLDIQAL
                     PDLSGFHQAITPLDQFDWVIFISSNAVQHGMPLLKQVGVPPSLKFAAIGPTTAASLHG
                     FGITQVLTPHERFDSESLLALDAFQNMQGQRVLIVRGVGGREVLAETLRQRGAEVVFG
                     ECYRRVNPQSSAQVLVQAYDEGKLQGIVVTSSEALRFLLDLGQSAPWLQGTPLFVNHA
                     RIAEQAQAAGLTAYCSGVSGDIAMLDLLLSELSVDS"
     gene            complement(5165..5824)
                     /locus_tag="VNZ84_09420"
     CDS             complement(5165..5824)
                     /locus_tag="VNZ84_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67220.1"
                     /translation="MFRLSVLLSLLATLPLAVQANTVISLENINCSGTLSISSLAGAS
                     LSCAGNFDLSDGFIESDSQIFILAEGDLLLENLNINAPDISISTLGGSLQLANTVNIK
                     ATQTEGNVGGKVMISVLPTKQDIHWNSFNIGLNSGANIQIGAPGTISSVPPSLTGAGE
                     IYVMNRNGIVFLNTNALNFTTAGFITINNPISISSVPEANVSAMLLLGFGLLTLRRRS
                     L"
     gene            5997..6302
                     /locus_tag="VNZ84_09425"
     CDS             5997..6302
                     /locus_tag="VNZ84_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWU67221.1"
                     /translation="MRTLTHIPEPWSEISELFVALGDAHRQRILLSFNRDERLTILQI
                     VESSQLSRTAVTHHLKVLERGGALQSEKIGREVYFWVNKAFMQQALQNVLDYIKDEI"
     gene            6331..8466
                     /gene="aas"
                     /locus_tag="VNZ84_09430"
     CDS             6331..8466
                     /gene="aas"
                     /locus_tag="VNZ84_09430"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HWU67222.1"
                     /translation="MLVNVLRGLCRLLFRVQVTGLENIPQEDRLLIIANHESFLDGLL
                     LGLFLPKRATFVVHTGVLKSIFFRWWLKLTPHLSVDPASPLAMKKVIQRLNAGENVVI
                     FPEGRITLTGALMKVYDGPGFVAAKTGVKILPVRVEGAAQSYFGRLSDAHPRKLFPRV
                     TLKVLPTTEIRIEQHGHHAPLTAKQRRRIAGEAMRNIMQHMLFKTQQSKTLFEALLDT
                     MEKYGAKTHIIEDMNQVEDTYQEVLKRSLALGRIATKVSQPDEVVGVLMPNVTNTLAL
                     VLGMTAFKRIPAMLNYTAGADGMRNACIAAKIRTVITSRKFIEAAKLQETVAQMSDLN
                     IVYLEDLRSQFGMLDRAWLMGYALLYPRAAMEPATAEDTAVILFTSGSEGKPKGVVHS
                     HKSILANTHQILAMLDFSPSDKFMMALPLFHAFGFTGALLPLLNGIPLLLFPSPLQYK
                     LIPEVIYDKGCTVFFSTSTFLSNYAKYAHPYDFYKLRVVFAGAEKLNEEVRKIYHEKF
                     GIRILEGYGTTECAPVLAANTPMANRNGTVGQFFPGIQYKLTPVPGIEKGGLLHVKGD
                     NIMQGYYLFDNPGVLVAPQDGWYETGDVVEVDAEGFIHIKGRVKRFAKVAGEMVSLEV
                     VEKIASTAAPDQQHAATTVVDASRGESIVLFTTDSKLKREDLQIVAKNLGSPEIAIAR
                     KIIVVEQIPVLGTGKTDYVTLKQMAEKAV"
     gene            8463..9680
                     /locus_tag="VNZ84_09435"
     CDS             8463..9680
                     /locus_tag="VNZ84_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU67223.1"
                     /translation="MNRRQFMMLGGAFVCGGLTQSAWKYFPDSGLTHPCEGAMPPALS
                     EHETWKTIWQDIRPDQVWDAHVHLVGVGDGSQQTWFNPAMDEWLHPQLKMQKAFYINA
                     TCADPAHIDNSSIARMDMLAQAMPAGVKLMLFAFDWHRDAQGQVLKQDSIFHISNQYA
                     ATVARRLPERYEWVASIHPYRADAVDALEHAYAHGAKAIKWLPSGMGIDPASARCDAF
                     YRKMAALGLPIISHTGQESAVPGGEQAFGNPLRMRRALEHGVTVVLAHCASDGMDQDL
                     DHGGRQVRSFELFKRLMETPGYEPLLYGDISATTLINHAWVLPELIQRTHWHARLIHG
                     SDYPLPGIMPLTNINTLIANGWLNDSHKPFLMALKNHHPLLYDFALKRLIVIDGQRFS
                     PTVFETRHIFERT"
     gene            9677..10999
                     /locus_tag="VNZ84_09440"
     CDS             9677..10999
                     /locus_tag="VNZ84_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU67224.1"
                     /translation="MSNPFHLLQQQRFGPFFWTQGLGAFNDNVFKTALITQVAFNTAS
                     LTTMDGATLATLLPGLFILPFFLFSASAGQLADKYEKSQIIRLVKVLEICIMLFASIG
                     FLSRSLLMLALALFLMGVHSTLFGPVKYSYLPQHLQPNELTAGNGLVEMGSFVAILLG
                     QVLGAWLGSLEQHALFTSLSVLLIAISGYHLSRGIPRSPAPVPDIKINWNPITETIKN
                     VRTFWAQQTLWVAIVAISWFWFFGATLLAQFPNLAKHVLQGSERDFISLLSIFSVGVG
                     VGSVWCEKLSKGKQELGLVMLGALGMSVFTWDFASVAHGIQQQLLANPALTLHTLAGL
                     DYWHMLLDVALLGISGGLYIVPLYVLLQSRAEAGYQSRVIAANNIMNALFMVISAGFS
                     VMLFGMGMSIAKLFAITAGLNVLVAIYLCFRQPEYWHSVRDYLSGLRP"
     gene            11002..>12095
                     /locus_tag="VNZ84_09445"
     CDS             11002..>12095
                     /locus_tag="VNZ84_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU67225.1"
                     /translation="MRRLFGVGLGLMSSFAQGEPGADHLGMHTQAQFQTVEGHCPQCQ
                     ALPQTLWYFRHETFAVPIPGLPVSRFDASKRGADDTQALNPEALPELIWTGSSQYWPQ
                     ATLNAAATEVTTVQGQRLNLKLVPKIDSNRSYWNADTSTYLSQSAVSIRGEIEGQTLV
                     ARTVWPQAFKLDLTVGLQQLQSGKTLQSLVQFANGGAQSPYQSRLLWEKAPGAAQSSS
                     GKAALGILLNGAQGDDDEAHGGHFALATGVVGAEGEYGTWLVNNYYNLATNSEKGIIA
                     GVTPFDKYMGDLNSGQAFYRPSYMLVAVFSQPDIPKQIQALNNRVMQHFYHNDLVYDH
                     ALENCAGISIDTLRHLGWQVPERGVESLLKA"
BASE COUNT         2881 a         2925 c         3275 g         3014 t
ORIGIN      
        1 acacgctgtc aaagcctaat aattcggcca ccagttcgcg cttgatcggg tagctggttc
       61 ctgcgagtgc tgctgcaccc aagggtaggc ggttgatacg tttacgtgca tcggcaaagc
      121 gctcggcatc gcggttgagc atttcaaaat aagccatcag gtgatgaccg aatgtcaccg
      181 gttgggcgac ttgcagatgg gtaaagcccg gcataatggt tgctgcatta ttttcagcca
      241 aatccagcaa cgaggtttgc agggcgcgga taccagcaat ggtgtcgtca accacgctac
      301 gtaaccataa gcgcacatct gtggctactt ggtcattacg tgatctgcct gtgtgtaagc
      361 gtttaccggc atcgccgatt ttttctgtca ggcggcgttc gatattcagg tgtacatctt
      421 ccaggtccag caaccactca aattgcccgg cttcaatttc ggccagaatt tcacccaggc
      481 cacgtttaat gtcttctaca tcctgtgctg aaataatgcc ttgcgcgccc agcatggtgg
      541 cgtgggcgat cgaaccttga atgtcgaatt tagccaggcg tttgtcgaag tccacagaag
      601 cggtatagcg tttgaccagt tcggcgaccg gctcattgaa acggccagac catgcctggt
      661 ctttttggtg tagcgatgat tgtgtgttgg gttgctgagt tgtcaaagca aaagttcttt
      721 ctgccagaaa attggcgtct agcgcatgct tgcgccactg gcgcttaata cttatgatgc
      781 tggagccaca ttataaatga acatcacgct gtgcgtaaaa ctagccgcat tcccaatttc
      841 cgcaatctgg gcatacactt acgtgtactg ttgctgcacc tgtgtttgct ggcgttgttg
      901 acggtgtatg acggccagtc tggctggaat gtgactttat ggctcaatga gctggcacgg
      961 atcggcagcc agtgggcgcc gccgctgttg ctggtgatgg gcgcactgac attggcttat
     1021 cctgtaatta gccgctggcc ataccggctg gcgctggcga gtattttcct gctggcaatc
     1081 agcctcaccg cttttttggc ctatggatac caccactgga tcacgccgct ggccactgcg
     1141 caactatggc agctcatgtt gttgtgcgtc gcagatgtac tggtgtgttt gtattacctg
     1201 gatttattgc aacgggctta ttcgccggca attgccgagg cgcgattgca agcgctgcag
     1261 gcacgcatcc gccctcattt tctgttcaac agcctgaatg cggctttgtc cctgatccgt
     1321 agccagccgc agcgcgcgga aaccgcactg gaggatatgg cagaactgtt ccgtgtattg
     1381 atggccgaca accgtgacct ggtgccgttg tcacaggaaa tcgccttgtg ccagcaatac
     1441 ctgaacattg aaaagttacg gttggatgaa cgcctgcaat atgactggca actgtcagac
     1501 cttccgtccg aaatgatgat cccgccgctg attttgcagc ctttactgga aaatgcggtg
     1561 tatcacggca tcgagccgca atcgcagggg ggcatgattg atatcgaaat caggcccagc
     1621 ggcaacagtt tgctattgcg tatcagcaac ccgttaccac cacgcagtga caagtctggc
     1681 ggtaataaaa tggccctcaa aaatattcgc gaacgtttgc gcctgcatta tgacctggaa
     1741 gcacaattac gccagtacga ggctgaccag cggtatatcg ttgaaatcct gattccgacg
     1801 cgtcccttgt ctgaggtggt gcatggctaa acagcgttta ttgattgtgg atgacgaacc
     1861 gctggccagg cagcggttac gtgacctggt aagcgatatt ggcggatatg aagtactcgg
     1921 tgaggctgct aacggggtgc aagccatcgc cttgttgcaa cacgcgcagg tggatattgt
     1981 gctggcagat atccgtatgc cggtaatgga tggaattgag ctggcacaac atttacggag
     2041 cttggagaag cctccccacc tgatttttac cacggcttat gatcatcatg ccttgcaggc
     2101 atttgaactc aatgcgattg attatttgct aaagccgatc aggagcgagc gcctggcaac
     2161 ggctctagcc aaggcacggc ccttgcagcc tgaacaatcg caggccttgc aaactttgca
     2221 acagacgcat gctcatttga gcatacagga gcgcggcaaa gtgctgctga ttccattgtc
     2281 ggatgtactg tatttccggg ccgagctgaa atatgtgacg gtgcgtacgc gcgagaaaag
     2341 ctatttgctg gaaacctcac tgaaccagct ggaacaggcc tatgcagagc agtttgtgcg
     2401 catacaccgc aatgcgctgg tggcacgcga tgcgattagc gggtttgaaa agcagcgtat
     2461 ttctgaaggc gaggaggctg gtgaaacgtc gtgggtcgtg ttaatccggg gcatcccgga
     2521 tacgctcacg gtgagccgcc gtcaccagca tatgattaag gccacctagg tggccttttt
     2581 acctgttact ttttcttttg tgcctgatca atgaccttgg ctgcccagtc gcggccaccg
     2641 aggccaaaag caatcgccag tgccaggaac acggcaccaa acacaatcac aaacagggag
     2701 tgaatcagct gcatgccgat acccagttgt tccagcgcaa tgaaaatcgc cagaatctgg
     2761 atgcaatatt cggcagaggt gctgatgatt aatgcctgtt caaacttgat actatgtaac
     2821 caggcaaata ccagtcggtt gataaagcgt gcaaacaagg taccgaaaat caccaccagg
     2881 atggccacaa tgatatgcgg caggtaactg gccagttctt tcaagagtgc tgcgacttcg
     2941 ttcaggccca aggagttggc gcaggtgatg acgaacagca aaatcaccag ccaatagagt
     3001 acttcactga taatgccgct cagtgtcact ttgtaattgg cgctacgcat aaaggcttcg
     3061 aggccggagc ttttcgcaaa cgtatcaaag tgcgtcagtt caagaatacg cttcgcgcct
     3121 agcctgacgg ctttggccac cagccagcca aaaaacagaa taacaatggc agcgagtaat
     3181 ttgggcacaa aatggaccag ttcgacccag aattgattaa gagaagaaag gaaaatgtcg
     3241 atttggtatt gcatggataa ttcctccatt tttatcatcg aatagtagct tttctagcta
     3301 cttctggaag gctgtttttc tgccagcata actcaagatg acccttttgg ggagcaaaag
     3361 gttcatcact gcaagtgatc atcgctggtc agccaaggga cactgaagtg tggccttgcc
     3421 gtgttaaaat gctgtgctaa atccatcata ggtaacccag ttgtgagcac atttaatccg
     3481 ccagccaaac ttgttattgc ctctcgcgaa agtgcacttg ccatgtggca ggccaaacat
     3541 atccaggccc ggttacaggc cttatatcca caatgtgacg tcagcatttt aggcatgacg
     3601 accacgggcg accagatcct ggattccccg ttgtcacgca ttggtggcaa aggcctgttt
     3661 gtcaaagagc tcgaaaatgc gctggcagat ggccgagctg atttggctgt gcacagtatg
     3721 aaagatgtgc ccatgcattt gccggaaggg tttaccctga cagccaccgg tgaacgcgaa
     3781 gacccgcgtg atgcatttgt ctccaatact tacagcagcc tggaagagtt gcccgaaggc
     3841 agtatcgtcg gtacctccag cctgcgacgc caaagccaga tacaggcgcg atttccacat
     3901 ttgaaaattg aatccttgcg tggcaatgtg caaacccgtt tacgcaagct ggatgaaggt
     3961 caatatgcgg ccattatcct ggcggcggcc gggctgatcc gcctggaatt gggcgaccgt
     4021 attaaaggct ttattgaccc ggcagacagt attccagccg tgggacaagg tgcactgggg
     4081 attgaaatca atgctgatcg taaggatctg gtagatgtcc tggcgccatt gaaccatgtg
     4141 gatacccaat tgtgcgtgga agccgagcgt ggcttcagtc gcgcactcgc aggcagttgc
     4201 accgtcccgt tgggggctta tgctactaaa cagggtgaca ccatcaccat gcaaggcttt
     4261 gtagccagcg tcgatggcca gcagatgctc aaggaaaccg tgactggtca tgccgcagag
     4321 gcagaaaagc tgggccagca attggccttg caactggtct cacgcggtgc tgataaaatc
     4381 ctcgcgtctc tggatggcgt gaaatgacct tgccattggc cggacgcacg attgctgtca
     4441 cgcgtcctcc tgagcaagcg accaaattga cggcagccat tgaggctgct ggcggtgtgg
     4501 tgatttcgtt tccgctactc gatattcagg cgctgccaga tctttccggc tttcatcaag
     4561 ccatcacgcc cctcgatcag tttgactggg tgattttcat cagtagcaac gccgtgcaac
     4621 atggcatgcc tttgctcaag caggtcgggg ttccgcctag ccttaaattt gccgcgatcg
     4681 gcccgaccac ggcggccagt ttgcatggat ttggcattac acaagtgctg acacctcatg
     4741 agcgttttga tagtgaatcc ttgctggcac tagatgcgtt ccaaaacatg caaggacagc
     4801 gtgtcctgat cgtgcgaggg gtaggcgggc gtgaagtgct ggccgagacc ttgaggcaac
     4861 gaggtgcaga ggttgtgttt ggtgaatgct accgccgcgt caacccacaa agtagtgccc
     4921 aagtgctggt gcaggcttat gatgaaggca aattgcaggg catcgttgtc accagtagcg
     4981 aggcgttacg ctttttactt gatttgggcc agagtgcgcc ttggttgcaa ggcacgcctt
     5041 tatttgtcaa tcatgcgcgt attgctgaac aagcccaggc tgctggcctg acggcttatt
     5101 gtagcggtgt ttctggagat attgctatgc tggatttgtt gctaagcgag ctctcggtag
     5161 attcttaaag cgatctgcgt cttaaggtca gcaaaccaaa tcctagtaac aacatcgcgc
     5221 taacgtttgc ttctgggaca gaggaaatgc tgattgggtt gttgattgtg ataaacccgg
     5281 cagtggtgaa attcaaagcg ttagtattta gaaatacaat cccgttacga ttcatcacat
     5341 atatctcgcc agcccctgtt agagagggtg gcacactgct aatggtgcca ggtgcgccaa
     5401 tttgaatatt ggcgcccgaa tttaggccta tattgaaact attccaatgt atgtcctgtt
     5461 tagttggtag caccgagatc atcacttttc cacctacatt cccctcggtt tgagtggctt
     5521 tgatattcac ggtgttggcc aattgcaggc tgcctcctag cgttgaaata gaaatgtcag
     5581 gcgcatttat atttaggttc tccagcagta agtcaccttc tgccagaatg aagatttgcg
     5641 agtcagactc gatgaatccg tcacttaaat cgaagtttcc tgcgcagcta agtgaggctc
     5701 cggcaagcga cgagatagac aacgtaccgc tacaattgat gttctcaagg ctgatcacgg
     5761 tattggcttg tacagccaaa ggcagtgtcg ccagcaacga taaaagtaca cttaagcgga
     5821 acatgtggat ttcctcatag atattgttct ttgtttttat gcatagtgta gtgaccagcg
     5881 caggatggtg aatgacggtt tagtgaaatg cggcgtatag gctgacttca cagttttcaa
     5941 cttgagtggg catggatgct tgacaaacta aactaaataa ctaatcatat agttatatga
     6001 gaaccctgac ccacatccca gagccctgga gcgaaatttc agagttgttt gtcgcgctgg
     6061 gcgatgcgca tcggcaacgg attttgctat cgttcaacag agacgaacgg ctgaccatcc
     6121 tgcaaattgt cgagagctca caactaagcc gtaccgccgt gacgcaccat ttaaaagtat
     6181 tagaacgtgg tggcgccttg cagagcgaaa agattggccg ggaggtttat ttttgggtca
     6241 acaaggcatt catgcagcag gcactgcaaa atgtgctcga ctatattaaa gacgaaatat
     6301 aaaaaagaga tctgacaggg agtaaaagca atgttggtaa atgtattaag aggcctatgc
     6361 cgtttattgt tccgggtgca ggtcaccgga ttggaaaata ttccgcagga agatcgttta
     6421 ctcattatcg ccaaccacga atcatttctg gatggcttgt tgctgggatt gtttttgcct
     6481 aaacgtgcaa cttttgttgt gcatactggc gtattgaaaa gtattttttt ccgctggtgg
     6541 ttgaaactga ccccacattt gtcggtggac cctgccagtc cactggccat gaaaaaagtg
     6601 atacagcgcc tgaatgctgg ggaaaacgtg gtgattttcc ctgaaggacg cattacgctg
     6661 accggtgcct tgatgaaggt atatgacgga cccgggtttg tggctgccaa gaccggcgtc
     6721 aagattctgc cggtacgcgt tgaaggtgct gcccagtctt atttcggtcg attgtcagac
     6781 gcgcatccgc gcaaactgtt tccacgcgtg accttaaaag tgttaccgac caccgaaatc
     6841 cgtattgaac agcacgggca tcatgcgcca ctgacggcca agcagcgacg ccgcattgcc
     6901 ggtgaggcca tgcgcaatat catgcagcac atgctgttta aaacccaaca gagtaaaacg
     6961 ctgtttgaag ccttgctcga tacgatggag aagtacggcg ccaagacgca cattatcgaa
     7021 gatatgaacc aggtggaaga tacctaccag gaagtgctca aacgcagcct ggcattaggg
     7081 cgcatcgcca ccaaagtcag tcagcccgat gaagtggtag gtgtgctcat gcctaatgtc
     7141 accaacacac tggcgttagt actggggatg accgctttca aacggattcc ggccatgctg
     7201 aactatacgg ccggcgccga cggcatgcgt aatgcgtgta tcgccgccaa gattcgtacg
     7261 gtgatcacgt cacgcaaatt tatagaggcg gccaaactgc aagaaactgt ggcccagatg
     7321 agcgatttga acatcgttta cctggaagat ttgcgcagcc agtttggcat gctggaccgc
     7381 gcctggctca tgggttacgc tttgctgtat ccacgtgccg ccatggagcc ggcgacggca
     7441 gaagatacgg ccgtcatcct gtttacctct ggttctgaag gcaagccaaa aggtgtggta
     7501 cacagccata aaagcatctt ggccaatacg caccagatcc tggccatgct ggacttcagc
     7561 ccgtcggata aattcatgat ggccttgcca ttgttccatg cctttggttt tacaggcgcc
     7621 ttgttacctt tattaaacgg tatcccgctg ttactgttcc cttctccctt gcaatacaag
     7681 ctgatcccag aggtgattta tgacaagggg tgtacagtct tcttctctac cagcaccttt
     7741 ttgagcaatt acgccaaata tgcgcaccct tatgactttt ataaattgcg cgtggtgttt
     7801 gccggagccg aaaagctgaa cgaagaagtc cgcaaaatct atcacgaaaa atttgggatt
     7861 cgcattcttg aaggatatgg caccactgag tgtgcgccgg tgctggcggc caatacgccg
     7921 atggcaaacc gtaatggtac cgtcggccag tttttcccgg ggattcaata taaattgacg
     7981 ccggtgccgg ggattgaaaa aggtgggcta ttgcatgtca aaggggacaa catcatgcaa
     8041 ggctattatc tgttcgataa cccaggcgtg ctcgtagcgc ctcaggatgg ctggtatgaa
     8101 accggtgatg tggtcgaagt ggatgctgaa gggtttatcc acatcaaggg acgggtcaag
     8161 cgctttgcaa aagtcgcggg tgaaatggtg tcactggagg tagtggagaa aattgccagc
     8221 actgcagccc ctgaccaaca gcacgcggca actacagtgg tagatgccag ccgtggagaa
     8281 agcatcgtgt tatttacgac cgatagcaaa ctcaagcgtg aagacttgca aattgtggcc
     8341 aaaaatctcg ggtcgccaga aattgccatc gcccggaaaa ttattgtcgt cgaacagatt
     8401 ccagtattag gcaccggtaa aaccgattat gtcacgttga aacaaatggc ggaaaaagcg
     8461 gtatgaaccg caggcagttc atgatgctgg gcggggcgtt tgtgtgcggt ggcttgaccc
     8521 agtcggcctg gaaatatttt ccagacagtg gactaacgca tccctgcgag ggcgccatgc
     8581 cgccagctct cagtgagcat gaaacctgga aaaccatctg gcaggacata cgccccgatc
     8641 aggtgtggga tgcccatgtg catttggttg gtgtgggtga tggatctcag caaacctggt
     8701 ttaatccggc catggatgag tggctgcatc cgcagctaaa aatgcaaaag gctttttaca
     8761 tcaatgccac ttgcgctgat cctgcccata tcgataactc ttccatcgcc agaatggata
     8821 tgctggcgca ggccatgcct gcaggagtga aattgatgct gtttgccttt gattggcacc
     8881 gggatgcaca aggccaggtg cttaaacaag attcgatttt ccatatttca aatcaatatg
     8941 ctgcaacagt tgcgcggcga ttacctgagc ggtacgagtg ggtcgctagt atccaccctt
     9001 accgggcaga tgcggtggat gcactggagc atgcttatgc acatggcgca aaagccatta
     9061 aatggctacc atcgggcatg ggaatagacc cggcctccgc caggtgcgat gctttctatc
     9121 gcaaaatggc tgcactgggt ctgccgatta tcagccacac agggcaggaa agtgccgtac
     9181 ccggcggtga gcaggcgttt ggtaatcctt taagaatgcg gcgtgcgctt gagcatgggg
     9241 tcacggtagt gcttgcccat tgtgcaagtg atggcatgga ccaggattta gaccatggcg
     9301 ggcggcaggt gcgaagtttt gagctgttta aacggctaat ggagacgccc ggttatgaac
     9361 ctttactcta tggtgacata tcggcaacca ccctgattaa ccatgcctgg gtcttgccag
     9421 agttgattca aaggacacat tggcatgcca ggttgataca tggctcagat tatcctttac
     9481 cggggattat gcctctgacc aacatcaaca cattgatcgc caatggctgg ctcaatgact
     9541 cgcataaacc ctttttaatg gccttgaaaa atcatcatcc gttgttgtat gactttgccc
     9601 tgaagcggct aatcgtcatt gatggccagc gtttttctcc cactgttttt gaaaccagac
     9661 acatttttga gcgaacatga gcaatccttt ccacttgtta cagcagcaac ggtttggccc
     9721 ttttttctgg acccagggcc tgggcgcttt caacgacaat gtgtttaaaa ccgcattgat
     9781 tacgcaggtg gcatttaata cggccagcct caccacgatg gatggagcga cgttggcgac
     9841 gttgctgccg ggtttgttta tcctgccgtt tttcctgttc tcagcctcgg ccgggcagct
     9901 ggcggataaa tatgaaaagt cgcagattat ccggttggta aaagtactgg aaatttgcat
     9961 catgttattt gccagcatcg gttttttgag ccgcagcctg ttgatgctgg cgttagcctt
    10021 gtttttaatg ggcgtgcatt cgaccttgtt tgggccggtg aaatattctt atttgcccca
    10081 gcatttacag ccaaatgagt tgacggcagg caatgggctg gtggaaatgg gcagctttgt
    10141 cgccattttg ctgggccagg tgctgggcgc ctggttgggg agtttggaac agcacgcctt
    10201 atttacaagt ttgagcgtgt tgctgattgc catctctggc taccacttga gtcgggggat
    10261 tccacggtct cctgcgcctg tgccagacat caagattaat tggaacccga ttactgagac
    10321 catcaagaat gtgcgtactt tctgggcaca gcaaaccttg tgggtcgcca ttgttgcgat
    10381 ctcatggttc tggttttttg gggcgacatt gctggcgcaa tttcccaatc ttgccaagca
    10441 tgtgttgcaa ggcagtgaac gcgactttat cagtttgctt tccatttttt cagtaggcgt
    10501 gggcgtgggt tcagtctggt gtgaaaagct ctccaagggc aagcaagagc tgggactggt
    10561 gatgctaggc gcattgggta tgtcggtatt cacctgggat tttgccagtg ttgcccacgg
    10621 tatccagcag caattgctcg ccaatccggc attgacttta catacactgg cgggattaga
    10681 ctactggcat atgctactgg atgtggcgtt gctgggaatc agtggcggtt tgtatattgt
    10741 gcccttgtat gtgttactgc aaagtcgcgc agaggcaggt tatcaatcac gcgtgattgc
    10801 cgctaacaat attatgaatg ccttgtttat ggtgatatct gctggcttct cggtcatgtt
    10861 gtttgggatg ggaatgagta ttgcaaaatt gtttgccatc acagcaggat taaatgtgct
    10921 ggtggcgatc tacttatgct tccgccagcc ggagtattgg cacagcgtgc gtgattacct
    10981 gagtgggttg aggccttgag aatgcggcga ttatttggtg ttggcctcgg cctcatgtcg
    11041 tcgtttgcgc agggtgagcc tggcgcagat cacttgggca tgcatacgca ggcgcaattc
    11101 cagacggtag aagggcattg cccgcaatgc caggctttgc cacaaacctt atggtatttc
    11161 cgccatgaga catttgccgt gccaatcccc ggtttgccag tatcccgctt cgatgccagc
    11221 aagcgggggg ctgacgatac ccaggcgctc aatccggagg ccttgcctga attgatctgg
    11281 acaggttcca gccaatattg gccgcaggcg acactgaatg ccgcagcaac agaagtcacc
    11341 accgtacaag gccagcgttt aaatttaaaa ctggtaccca aaattgatag caaccgttca
    11401 tattggaacg ccgatactag tacttaccta agccaatcgg cggtttccat tcgcggcgag
    11461 atagagggtc aaaccctggt ggcacgaact gtatggccgc aggctttcaa gctagattta
    11521 acggtcggcc tgcaacaact tcaaagcggc aagaccttgc aatcactggt tcaatttgcc
    11581 aatggcggcg cacaaagccc ataccaaagc cggttgttgt gggagaaagc acctggggca
    11641 gcacaatcgt cttccggaaa agctgcgttg ggaattttgc tcaatggtgc ccaaggcgat
    11701 gatgatgaag cgcatggcgg gcattttgca ctcgccactg gcgtggtagg ggcggagggt
    11761 gaatacggca cttggctggt caataattat tacaatctgg ccaccaatag cgaaaaaggc
    11821 atcattgcgg gcgtcacgcc ttttgataaa tacatgggtg acctcaacag cgggcaagca
    11881 ttttaccggc cttcttatat gctggtggcc gtgttcagcc agcctgacat tcccaaacaa
    11941 atacaggcgc tgaataaccg tgtcatgcaa catttttacc ataacgactt ggtgtatgac
    12001 cacgcgctgg aaaattgtgc cgggatcagc atagacaccc tgcgtcacct tggttggcaa
    12061 gtacctgagc gaggggtgga aagtctgctc aaagc
//
